LOCUS       DAOMIV010000001        19074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_2829_length_19074_cov_4.508439, whole genome shotgun sequence.
ACCESSION   DAOMIV010000001 DAOMIV010000000
VERSION     DAOMIV010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 19074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19074
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2829_length_19074_cov_4.508439"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            699..1550
                     /locus_tag="PKV59_00005"
     CDS             699..1550
                     /locus_tag="PKV59_00005"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HQP45159.1"
                     /translation="MAVIHEANRKFDRKNIYTKWILPDDQPVANIILVHGIGEHCARY
                     DHVTAFFADHHIAVYTFDQIGHGKSEGKRGAMTYDQTWGIINEIKAELIQKEPAIPVF
                     LYGHSMGGAIVLSYAYRYPENLAGVIATAPAIGLANPTSGFMTAMARFLSKIVPNMTI
                     SNGLALDGLTHDKKIVELYQNDPLVHDKVSFKLAVDLIDKGNEILKSADKFPVPLLLL
                     QGTDDKLVDPKATAQFAAHLNQNGTYKPIEGGYHELHNEPYKEEIFNIILQWINDRLN
                     EPEKENA"
     gene            1738..4863
                     /gene="gcvT"
                     /locus_tag="PKV59_00010"
     CDS             1738..4863
                     /gene="gcvT"
                     /locus_tag="PKV59_00010"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQP45160.1"
                     /translation="MENYFFNGDLTDIDNAIARLTKLEAERQKRKLIMIPSESFAPQA
                     VRESLGSVFQNVYAEGYPPEEMRSLTENEILDYKARLGDYRRNSDPRYYKGVEYIDIL
                     ESLARRRCAELFANDRISADEIQVNVQPLSGAPANNAVYHLINPGDTVLAMDLLHGGH
                     LSHGSPVNRSGMLYNIVHYTVNPETQLLDYDEMQRLASEYKPRMVIAGYTSYPHIPDW
                     ERYRQICDSVGAILMADIAHIAGLVAAGVVASPVGYADVITFTSHKTLTGPRAAVILT
                     TDPELSKRIDKAVFPGEQGGPHTQVFAALATNFKIAATPEFKVYQQQILNNTQAIIKQ
                     LQSRGIKVAYQGSNSHMLLVDCKCIKGEDGTTLSGDMAARILDNAGIVLNRNTIPGDK
                     SALRASGIRMGTSCITQIGLKEEESAQVADIIADLLFAIHPYSMPGAKGLLQRAKIDF
                     DVFESCKTRVRRLCEKHNKFTDSDEFGYPHFYYIDDSFLSEQGTSYLLISGDKSQEFV
                     NFAFQNDAESLSLNECQDAVISVNGELINGCLMLREDGYQFGCPSEKINVVAAWLRDL
                     SDGFIRFDDDLRMKLPGPVFISETIGIKGSESVISEFEKPYAIGKKRKEGIGLPEFDF
                     SIYENQETQPSKLTPLHDIHIALGAKMMPFAGWDMPLWYTSIKEEHQAVRNAAGLFDV
                     SHMGVYQVSGKDACAFLDSVCGNDISALEVGDTCYTHFLDLDANVIDDLIVYAIEPQK
                     YLVVVNASNDDKDWAWLNAVHNDEILIDRESPLSRAFGRNSILENLRSPEAGERMRVD
                     LALQGQASKRILAALDAAPEDRKKIDKLEKGHVTHVNLAGFDLYIARTGYTGEKISYE
                     LFVHPAKLPELWNKLLEAGKADGLMPCGLGARDSLRIEAGLPLYGHELAGPMNYRVNE
                     AGFSHFVKTYKPWFIGRNAYLKHVKEQKQEIVRFKFNEKAVRMAHTNDPVLDSKGRVI
                     GRVTSCSVDKDGWIIGMAMIESAYKQEGTSIFIYQNSEKLELKDPSSLQPGDRTVIPS
                     QATIISRYPKL"
     gene            complement(4924..6633)
                     /locus_tag="PKV59_00015"
     CDS             complement(4924..6633)
                     /locus_tag="PKV59_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HQP45161.1"
                     /translation="MDQRFKIIQPTNIIQAELPDGRLIEGPRGGTVEEFLQPIVSELE
                     FPLVAAIVNKELRELSYPLISNANIEPVTTAQSDGKNIYRRSLVYLLSATFERMMPDY
                     ELMVDHSIHSGGLFCRIKGPELQDPKGFYKMLKTEMQATIDANLPILRVKLTIEEAQA
                     LFTSKHQQEKADLLKYRKKDYLTAYKLGDFVDYHYGYMVPSTGYLKVFGIEPAGRPNE
                     FFLRYPSDGKSNVIQKLDESSKMMSEFRRYGDWLQRLGIDSIASLNQAIESDRIQEVI
                     LVSEALHDQKFVQIARDIVERKNEVKVVLIAGPSSSGKTTFSKRLSIELLALGISPIP
                     IEMDDFFVDRERSPRTPDGNYDFESIDNLNLDLMDRCTDRLIAGEEVQLPRFDFFDGK
                     SKPGKILKMKKDQLLIFEGIHGLNPKLLPGISPEKKYRIFVSPLTQLNVDRYNRVSTV
                     DTRLIRRVVRDYRDRGYSAQETIARWESVREGEEKYIIPHQRLADEYCNTSLVYELSA
                     LKPMAETALRQVQWGSWEYIEAKRLLSFLEWVLPLPDGLVPANSILAEFIGGSNLKNF
                     TVW"
     gene            6705..7337
                     /locus_tag="PKV59_00020"
     CDS             6705..7337
                     /locus_tag="PKV59_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HQP45162.1"
                     /translation="MTKMTIFIILTILLLIRIIIGLWAKKRIFSLTSIPRNEYGIVFG
                     AGLEKTGLPSNVLMERIQTAAMLYRAGKIRKVLLTGDNHSLYYNEPQAMKIAAMTLGI
                     SEKDILVDPNGHRSFDSCRNAICDFNIHQAVLITQPFHLPRVLWLAVSVGIDAIGMPV
                     DLKRHRIDDEIWWRFRELFASVRAIIDGFIYKICYSVNEKRMNDRKGEIK"
     gene            7334..8185
                     /locus_tag="PKV59_00025"
     CDS             7334..8185
                     /locus_tag="PKV59_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQP45163.1"
                     /translation="MSKDIGFFTLNNNVRIPKIGFGTYKAAENGDESVIKEAIRQGYT
                     LFDTASFYENEAIVGSAVKKSGLPRDHFFLTSKLWKTQMGYQNTLQAFNDSCKQLKTD
                     YLDLYLIHWPRPDLVCDWKKIVVETWKALEELYDEKKVRAIGVSNFLPHHLDVILNSC
                     KIMPAVDQIEFHPGYTQTETVNFCRSLRIQVEAWSPIGRARLIDNPLLKEIAAKYNKS
                     VPQLCIRFSLQNNVLPLPKASSPERMAQNLDVFDFEISEEDMEKIMAMPVTGWSGEHP
                     DAEPVAI"
     gene            complement(8359..9471)
                     /gene="mtaB"
                     /locus_tag="PKV59_00030"
     CDS             complement(8359..9471)
                     /gene="mtaB"
                     /locus_tag="PKV59_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HQP45164.1"
                     /translation="MKVFLDSVGCRLNQAELEQFAAKFKQYGYEIADSADEADLAIIN
                     TCSVTKEAASDSRGKIRNAARSGHTQVIVTGCWSETEPEAASKMPGVTQVIRNHEKEQ
                     IASIVTGIPEPFINPVSIKREILPGEHFRNRAFIKVQDGCDNYCTFCLTRLARGKSRS
                     VAAETILQDIRAAENGGGKEIVLTGVNLGAWGMDFNPPQGLRDLLNRLLAETSIPRIR
                     LSSLESWNLDEAFISLWENKRLCPHLHLPLQSGSEAVLKRMARRNSLPDFLRLIQCVR
                     QINPLFSVTTDIIVGFPGETDEDFRRTLDFVRTAAFSGGHVFTYSVRPGTAAAKLDGR
                     IPKDVKRERSKILREQLDEQRKSFGMKMIGKTTNVL"
     gene            complement(9468..10295)
                     /gene="proC"
                     /locus_tag="PKV59_00035"
     CDS             complement(9468..10295)
                     /gene="proC"
                     /locus_tag="PKV59_00035"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQP45165.1"
                     /translation="MNSEKMAIIGIGVMGKAILTGILKKGLISPQNISVYDTDADKMR
                     SAYEELGVNPSDSGTDAIRDADVILIAVKPQFLSEVFGELKNKIHPDALVISIVAGVP
                     IEQFQKGLDHERVVRVMPNTPAQIGEGVSGWFASREVAADQRNLAQEILACTGYAFEL
                     KSEDQLDQITAVSGSGPAYVFLFIEAMIDTAVHLGLSRPLATEIIIRTVKGSTDYLAM
                     RKEHPAVLRNEVTSPGGTTAEAVFELEKLGFRNAIAEAMWACYERTIELGKGSEGSH"
     gene            complement(10299..11198)
                     /locus_tag="PKV59_00040"
     CDS             complement(10299..11198)
                     /locus_tag="PKV59_00040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQP45166.1"
                     /translation="MNNKYLPEVKLLESPDWKDYMLLDSGAGRKLERFGRYILIRPEA
                     EAMWSPLLPETEWNKASAEFKPSPEENGGHWIFYEKDIHPWNIGYKNLKFQLMLSNSK
                     QVGVFPEQACQWDWVSSLIRNSEKPVKVLNLFGYTGAITMAAAEAGAEITHLDASKKA
                     VDWAKENQKLSSLEQTPIRWIIDDGLKFVQREARRGSFYRGIILDPPKFGRGPKGEVW
                     EFYKNFPELIQACRNILDSEALFLLVTAYAVKASSVTVYNTVNEVMKGMGGSVSAGEV
                     CLKDKSGGRLLSMAVYSRWQKEG"
     gene            11341..12255
                     /locus_tag="PKV59_00045"
     CDS             11341..12255
                     /locus_tag="PKV59_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45167.1"
                     /translation="MTKIIEVDKLSKSYGPVKAVQDISFYVEKGDLFAFLGPNGAGKS
                     TTIDIITTVLMPDKGTVIVDGLVLGKEDDKIRSSIGVVYQDNLLDRLLTVEENLFFRA
                     GFYGFNKDRIRESVKNAILTTGIQDYAKQRYGKISGGQRRRADITRALINTPQILFLD
                     EPTTGLDPQTRKNLWEVIRKLQRDREMTIFLTTHYMEEAAEADYVVVIDEGKIAARGT
                     PFELKEKYTSDLLKLSFKDEIKGLSDLADWGITYTKAGDQFIIRLPHTTDALPILDRC
                     RENLNGFEVLNGTMDDAFIGITGKEIRE"
     gene            12252..13109
                     /locus_tag="PKV59_00050"
     CDS             12252..13109
                     /locus_tag="PKV59_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45168.1"
                     /translation="MIALASRNIKLFFRDKAMVFFSLLSVFIIIGLYVLFLGNVWTSE
                     FPNQEYAKFIMNSWIMAGLISVTGFTTAVGAFGIMIQDRELNIEKDFLSAPVKRSSLA
                     AGYLLSAYGISVIMSLIAFILGEIFILAEGGELLGIVAIFKIIGIILINSFMSVSIIQ
                     VIIGFIHTGNAFSAFSTIIGTLIGFLTGIYLPIGQLPTGVQTLIRIFPVSHSASLARQ
                     IFMDVPLKIGFNGVPEAAVNGFKELMGVTFFVGSKEITPWMSLCYMLLIGALFFAIAV
                     MNLSKRKLE"
     gene            complement(13272..14564)
                     /locus_tag="PKV59_00055"
     CDS             complement(13272..14564)
                     /locus_tag="PKV59_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQP45169.1"
                     /translation="MKTLFSNVDILTPKYSQGVIRDGYLGITNEIISYIGETKPIEEY
                     DVERNFYNCLIIPGLYNMHTHTPMAALRGLGTNLPLDRWLFEAMLPIEQKMTPADKSV
                     GVRLEMMEMLASGVVSFSDMYHYPNHYIEDVIQSGMKANLSFPIMAVMASDSDKNKEI
                     MRLSVEFFDEYNGSADGRIMTDFAIHAEYTNNVEIVREYSEICRGKNGRMHIHLSETK
                     KEHEECKKRYGKTPAAWFYDLGTFDNPTIAAHCVMVEPEDILLLKEKDVTVVHNPSSN
                     MKLGSGVMPIRKMKEKGLRITIGTDSSASNNNQNMIEEMALTALIHCGFSQDPTVLSP
                     NEVLTMATLNGAAAQGRSNCGELEVGKKADLAVIDLDKPHLMPIHDYPSLIVYSAQAA
                     DVIMTMVDGRTLYEKGEFLSIDKDRVKFDLKKSLERLF"
     gene            15046..17775
                     /locus_tag="PKV59_00060"
     CDS             15046..17775
                     /locus_tag="PKV59_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQP45170.1"
                     /translation="MEKKTLIVNGIRQVVIHNPDAMLADVLRNQLHVLGVKKGCGQGH
                     CGACSIILNGKLVLSCIHKMSRIPDESTILTVEGIGTPGHLHPIQTAMKFHSAAQCGF
                     CTPGFVISSYALLQENPNPTRQQIRDWFTEHHNVCRCNGYKPYIDSVIDAAAVLRGEK
                     SIEEITYKEPKDNKVWGTRRPRPSLDAKVTGTWDFGADVAAKMPSNTLHCALVQSQVP
                     HANIISVDTSEAEKAEGVFKVVTAKDVKGRNQITGLITFPTNKGDGWDRPILCDKKVF
                     QIGDAIAIVCADTQEQADAAAKLVKVELEILPAYMNAPAAMAEDAIEIHPGTPNIYFE
                     QKNVKGNDTKPIFEKAAYTVEGNYYLQRQPHLTVEPDNGFAYIDEDGNLTIHSKSIGI
                     HLHAAMIAEGMGVPLDKLRIVENNAGGTFGYKFSPTMEALVGVACLATQRPVVLIYNY
                     YQHMIYTGKRSPMFLKCRMAADENGKLLAMEGDWSVDHGPYSEFGDLLTLRVSQFMGA
                     GYNIPNIRSIGRTVCTNHIWGSAFRAYGSPQAFLASESCIDELAEKMGIDPFELRYRN
                     IYREGSTTPTGQKPDSLCFEEMFDTMRPKYEAAKAKAKAESTPEYKKGVGISLGVYGC
                     GLDGADSSEAIAELSPDGKVIIYSSWEEHGQGNDAGALGTAHEALRKAGFKPEQIKLV
                     FNDTKITPNSGPAGGSRSQTMTGNAIRIACENLLDQMRKADGSYRTYDEMKADGKETK
                     VTGKFVMSEATSCDENGQGRPFMVYQYGLFMAEVTVEIATGKTKVDKFTCFGDCGVIN
                     NRAIVEGQMLGGCAQGIGLALSEDIEDYNKHTTMRGAGIPYCNDIPDEWDIEMFENPR
                     EYGPFGAGGVGELPLTASHVAVINAINNACGVRIRHLPAYPEKVLEGLKALNK"
     gene            17894..>19074
                     /locus_tag="PKV59_00065"
     CDS             17894..>19074
                     /locus_tag="PKV59_00065"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQP45171.1"
                     /translation="MDQQFMRTMESICIQNEPPACVTACPLHVDSRTMLKLVASGKFE
                     DAYSLYQKNVPFPSILSKICNSPCLSKCRLSEIGEGIQIRPIEAFLNQYADPSFLPKV
                     LPEKRQKAGIFGSELDSLSAAYDLRRKGYQVTILDADAVLAKNLRAFNEEVLPSVEIE
                     NAIHLLEKIGVKIQNDFEIPRFEELSRYDSGFFRIGDSEFQCIYLPPLSGELSIDSIT
                     RMTVTEGVFSGSASAGYWVDQMSDGRRAAISMDRYLQKVSLTASRENEGAYECRLFTS
                     LKGIQPSESFISTDIKRTGQADIIKEAARCIQCQCLECAKGCEFIRHYQDSPRTYVRQ
                     IYNNISICTGLRQKNKMINSCSICGQCEVICPNHIPFQKVVREARETMVATKKMPPSA
                     F"
BASE COUNT         5320 a         4340 c         4136 g         5278 t
ORIGIN      
        1 ccttgagagg gaagccctct cacccccaat cctttccggc ttgccgaaaa tcgctctcta
       61 tctttcaacc gactcgattt tgtgaaggac cttgagaggg aagccctctc agcccacccc
      121 gctttccggc ttgccgaaaa tcattccgca tctttcaacc gactcgattt gttcgaagga
      181 ccttgagagg gaagccctct caccgttccc ccctttccgg cttgccgaaa atcattccgc
      241 atctttcaac cgattcgatt tgttcgaagg accttgagag ggaagccctc tcagcccacc
      301 ccgctttccg gcttgccaaa aatcattccg catctttcaa tcatctcgac tttatgaagg
      361 accttgagag ggaagccctc tcacctcttc ctctttccgg cttgccgaaa atcattcctc
      421 atctttcaat cgactcgatt ttgtgaagga ccttgagagg gaagccctct caccgctgcc
      481 cccctttccc ggcttgccga aaatcgctcc ctatctttca atcatctcga ctttatgaag
      541 gaccttgaga gggaagccct ctcaccccca atcctttccg gcttgccgaa aatcgctacc
      601 catctttcaa atctcctccc ttcggaaact tgttaaaaca agatttcttc gctatacttt
      661 tattatagaa aaactcagaa atatccagga ggcataaaat ggctgtcatc catgaagcaa
      721 accgcaaatt tgaccggaag aatatctata cgaaatggat tcttccggac gatcaacctg
      781 ttgcgaatat catcttggtc cacggtatcg gagaacactg tgctcgatac gaccatgtca
      841 ccgccttttt cgctgaccat catattgccg tctatacctt cgaccaaatc ggacacggga
      901 aaagcgaagg gaaacgcgga gcgatgacct acgatcaaac atggggaatt atcaatgaaa
      961 taaaagcaga attgatccaa aaggaaccgg caattcctgt ttttctctac ggacattcta
     1021 tgggaggcgc aattgtttta tcatatgcct atcgctatcc ggaaaacttg gcaggtgtga
     1081 tcgcgaccgc tcccgcaatc gggttggcaa accccacctc gggattcatg acggcaatgg
     1141 ccaggtttct ctctaaaata gtcccgaata tgaccatttc gaacggactg gcacttgacg
     1201 gattaacaca cgacaagaaa attgtcgagc tgtaccaaaa cgacccattg gtccatgaca
     1261 aagtctcttt taaattggct gtcgatttga tcgataaagg caatgaaatt ctgaaatccg
     1321 ccgacaaatt ccccgttcct ttgttattac ttcaaggtac cgatgacaag ttggtagatc
     1381 cgaaagcgac cgcgcaattt gcagcccatc tcaaccaaaa cggtacttat aaacccatcg
     1441 aaggcggcta ccatgaactg cataacgagc catataaaga agaaatcttt aacatcattt
     1501 tgcagtggat caatgacaga ttaaacgaac ccgaaaagga aaatgcttga atcgattttc
     1561 cgcttcatat gagaagaacg gttcaatctg tccttgaatt tgcggtagat aattctcgag
     1621 cgcagctttt cgggaatcat aattataaga attcgaaaga tgtgccgaga aataacggta
     1681 gaattaaatc atctttaaca attccatcac aacggatcct ttggggaggc attatccatg
     1741 gaaaattatt tttttaacgg tgacctcacc gacattgaca acgcgatagc ccgattaaca
     1801 aaacttgaag ctgaaaggca aaaaagaaaa ctaataatga ttcccagtga atcgtttgct
     1861 ccgcaagctg tcagggaatc cctcggttcc gttttccaaa atgtttacgc cgaaggttat
     1921 ccgcccgaag aaatgcgttc tctgactgaa aatgagatct tagattacaa ggcaagatta
     1981 ggtgattatc gcagaaattc tgatccgcgg tattacaaag gagtcgaata tattgatatc
     2041 ctggaatcgc ttgcccgccg caggtgcgcg gaactttttg cgaatgatcg cataagtgcc
     2101 gatgagatac aagtcaatgt tcagccgctg tccggcgctc cggcaaacaa tgcggtctac
     2161 catttaatta atcccggcga tacggttttg gcgatggatt tgcttcacgg cggacacctt
     2221 tctcatggca gcccggtcaa tcgctccggc atgctctaca acattgttca ctacacagtc
     2281 aatcccgaaa ctcaattatt ggactatgac gaaatgcaac ggttggcatc ggaatataaa
     2341 ccgcggatgg tcattgcagg atacacttct tatcctcaca ttcccgactg ggaacggtat
     2401 cggcaaatat gcgacagcgt tggagccata ttgatggccg acattgctca tatcgccgga
     2461 ttggtcgccg cgggcgtcgt cgcttcaccg gtcggatatg cagatgtcat taccttcacc
     2521 tctcataaga ctttaaccgg accgcgcgct gccgtaatct taaccactga ccccgaactt
     2581 tccaaacgga ttgataaggc ggttttcccg ggagaacagg gaggacctca tacccaggtc
     2641 tttgccgcgt tggcgacgaa tttcaaaatc gctgccacac cggaattcaa ggtttaccag
     2701 caacaaattc taaataatac ccaggctatt attaaacaat tgcagtctcg cggaatcaaa
     2761 gtggcgtacc agggctcaaa cagccatatg cttttggtgg attgcaaatg tattaaaggg
     2821 gaggacggta ccactctatc cggtgatatg gcggctcgta tcttagacaa tgcgggtatt
     2881 gttttgaatc ggaatacgat ccccggagac aaatcagctc ttcgcgccag cggcattcgc
     2941 atgggaacat cctgtattac tcaaatcggt ttgaaggaag aagaatccgc tcaggtggct
     3001 gatattattg ccgatctgct tttcgcgatt cacccttatt cgatgccggg agcaaaagga
     3061 ttgcttcaaa gggctaaaat cgatttcgac gttttcgaat cttgtaaaac tcgtgttcga
     3121 cgcttatgtg aaaagcacaa taagtttacc gattccgacg aattcggtta tccccatttc
     3181 tattacatcg atgattcgtt tctgtctgag caaggaacct cctatctgtt gatcagcggc
     3241 gacaaaagtc aggaatttgt caatttcgcc tttcaaaatg atgccgaatc gctgtcgctg
     3301 aatgaatgtc aggatgctgt tatctctgtc aacggtgagc tcattaacgg ttgtttgatg
     3361 ctgagagaag atggatatca gttcggttgt ccgtcggaaa aaattaatgt ggttgccgct
     3421 tggttgcggg atctttcgga tggttttatt cgtttcgatg atgatttgag aatgaaactt
     3481 ccgggtcctg tatttattag tgagacgatc ggaatcaagg gcagtgagag cgtgatcagt
     3541 gaattcgaaa aaccttatgc gatcgggaaa aagcgcaaag agggaatcgg tttacccgaa
     3601 tttgattttt ccatctatga aaatcaagag acccaaccgt cgaaactcac gccgcttcat
     3661 gatattcata tcgctttagg cgcgaaaatg atgcctttcg ccggttggga tatgcccctt
     3721 tggtatacct cgatcaaaga agagcatcaa gcggtacgca atgccgccgg attatttgat
     3781 gtgtcgcata tgggtgttta tcaggtttcg ggaaaagacg cctgcgcctt tttggacagc
     3841 gtatgcggaa atgacatttc agcattagaa gtcggagata cttgctatac acatttcttg
     3901 gatttggatg cgaatgtaat cgatgatctc atcgtctatg cgatcgaacc gcagaaatat
     3961 ttggttgtgg tcaacgcatc gaatgatgac aaagattggg cttggttgaa tgccgttcat
     4021 aatgatgaaa tattaattga tcgagaatcc cctctctcac gggcattcgg ccgaaacagc
     4081 atattggaaa atcttcgcag tccggaagcg ggggaaagga tgcgcgtgga cttagcttta
     4141 caaggccagg cttccaaacg gattttagcc gcgctcgatg ccgctccgga agatcggaaa
     4201 aaaatcgaca aactggaaaa aggtcatgtc acccatgtta acttagccgg atttgatctc
     4261 tacattgcgc gcaccggtta tacgggagaa aaaatttctt atgaactttt tgttcatccg
     4321 gcaaagcttc ccgaactttg gaacaagctg cttgaagccg gtaaagctga tggtttaatg
     4381 ccttgtggtt tgggtgctcg tgattccttg cgaatcgaag ccggattacc gctttacggg
     4441 catgaattgg ccggtccgat gaattaccgt gtcaatgaag cgggtttttc ccattttgta
     4501 aaaacctata aaccttggtt catcgggcgt aacgcttatc tgaaacatgt aaaagaacaa
     4561 aagcaggaaa tcgtgcgctt caaattcaat gaaaaagccg ttcgaatggc acataccaat
     4621 gatccggttt tggattcgaa gggaagggtt atcggtcgcg tcaccagttg ttcggtggat
     4681 aaggatggct ggattatcgg aatggccatg atcgaaagcg cctataaaca agaaggaact
     4741 tccatattca tttaccaaaa cagcgaaaaa ttggaattga aagatccgag ctcacttcaa
     4801 ccgggagatc gaacggtaat tccgagccag gcaacgatta tcagtcgcta ccctaagtta
     4861 taaaatccga caaaagggaa caaaaagaat cttaataatg tttttgttcc ctttttgttt
     4921 tgattaccaa acggtaaaat tcttcaaatt ggatccgccg atgaattccg ccaaaataga
     4981 attcgccggg actaacccat cgggtaaagg caaaacccat tcaaggaatg agagcaaacg
     5041 ttttgcctcg atatattccc aggatcccca ctgcacctgt cttagagcgg tctcggccat
     5101 cggtttcaga gcagacaatt catagaccaa tgaggtattg caatattcat ccgccaaacg
     5161 ttggtgcggg ataatgtatt tttcttcgcc ttcacggacg gattcccacc gtgcgatcgt
     5221 ttcttgagcg ctgtagccgc gatcccgata atctctcacc actcggcgga tgaggcgggt
     5281 atctaccgtc gagacccgat tatatcgatc cacatttaat tgtgtcaggg gcgatacaaa
     5341 aatccgatat tttttttcgg gggatattcc cggtaacagt ttcggattga gtccgtgaat
     5401 tccttcaaag atcaataatt gatctttctt catttttaat attttcccgg gtttactctt
     5461 cccgtcaaaa aagtcaaaac gaggcaattg tacttcctcg ccggcaatta aacggtcggt
     5521 acaacgatcc atcaaatcca aatttaaatt atcaatcgat tcaaagtcat aatttccgtc
     5581 gggagtccgt gggcttcgtt cccgatcgac aaaaaaatca tccatttcga tcggaatcgg
     5641 tgagattccc aaagctaaaa gttcgatcga taacctcttg gaaaatgtgg tttttccgga
     5701 ggaagacggt cccgcaatca agaccacctt gacttcattt ttacgctcga cgatatctct
     5761 cgcaatttgg acaaactttt gatcatgcag agcttcgctg accaaaatca cttcctgaat
     5821 acggtcactt tcgatagctt gattcaggga cgcgatcgag tcaatcccaa gccgttgcaa
     5881 ccaatcccca tatcgacgaa attcagacat catcttcgag gattcatcca atttttgaat
     5941 cacatttgat ttaccgtcgc tgggataacg caggaaaaat tcattgggtc tgcccgccgg
     6001 ttcaattccg aaaaccttca ggtaacctgt cgaagggacc atgtacccgt aatgataatc
     6061 aacgaaatcc cccaacttat aggctgtcag gtaatctttt ttccgatatt tcaataaatc
     6121 ggctttttcc tgttgatgtt tcgatgtaaa aagggcttga gcttcttcaa tcgtcagctt
     6181 tacccgcaaa atgggtaaat ttgcgtcgat agttgcctgc atttccgtct taagcatttt
     6241 ataaaatccc ttcggatcct gcagttctgg tcctttgatt ctgcaaaaca agccgcctga
     6301 atgaatggaa tgatctacca ttaattcata gtcaggcatc attcgttcga atgtcgccga
     6361 gagtaaatag acaagcgatc tgcgatagat atttttgcca tccgattgag cggttgtaac
     6421 cggttcgata ttcgcattac tgattaacgg gtaactgagc tctcgtaatt ctttattgac
     6481 aatcgccgca acgagcggaa attccaattc cgaaacaatg ggctgcagaa attcttccac
     6541 tgttccgccg cgcggtcctt caatcaatcg tccgtcaggc aactctgctt gtataatatt
     6601 agtaggttga atgattttaa atctctgatc catgatgcct cattttctta ttgattatat
     6661 tcggaatcgg ttgaatggat tgattttaca ggattaaaat atcgatgaca aagatgacaa
     6721 tttttattat cctgaccatt cttttgttga tccgaatcat catcggtctt tgggcaaaaa
     6781 aacggatttt ttcgttgaca tccatccccc gcaacgaata cggtatcgtt tttggcgcag
     6841 gcttagaaaa aacagggctt cccagcaacg ttctgatgga aaggattcaa actgccgcaa
     6901 tgctttatcg agccggaaag atcagaaaag ttctgttgac gggggataac cattcgcttt
     6961 attataatga accgcaagcc atgaaaatcg cggcaatgac tttgggcatc tccgaaaaag
     7021 atattttggt cgatccgaac ggacaccgtt cgttcgattc atgccgaaat gcgatatgcg
     7081 actttaatat tcatcaagct gttttaatca cgcaaccttt tcatttgccg cgtgttcttt
     7141 ggctggcggt cagcgtcgga atcgatgcga tcggtatgcc ggtagacctg aaaagacatc
     7201 ggattgatga tgaaatctgg tggcggttcc gggagctgtt tgcttcggtc cgggcaatta
     7261 ttgatggttt catttataaa atatgctatt ctgtaaacga gaaaagaatg aatgacagaa
     7321 aaggagaaat aaaatgagta aagacatcgg atttttcact ttgaataata atgtcagaat
     7381 tcctaaaata ggttttggta cctataaagc agccgaaaac ggagatgaat ccgttattaa
     7441 ggaagcgatc cgtcagggtt atacattatt tgataccgct tcattctatg aaaacgaggc
     7501 cattgtcggt tctgctgtta aaaaatccgg cttgccaaga gatcatttct tcctgacttc
     7561 gaaactgtgg aaaactcaga tgggctatca aaatacgttg caggctttta atgattcttg
     7621 taaacagttg aaaaccgatt atctggatct gtatttaatt cattggccgc ggcctgacct
     7681 cgtatgtgat tggaaaaaga ttgttgtgga aacttggaag gctctcgaag aattatatga
     7741 tgagaaaaaa gtgcgggcaa tcggagtcag taactttctg ccgcatcatt tggatgtgat
     7801 tttaaattcc tgtaaaataa tgccggcagt cgatcaaatt gaatttcatc cgggatacac
     7861 ccaaacggag acggtcaatt tctgccggtc gttacgaatc caggtagaag catggagtcc
     7921 gatcgggcga gcccggctga tagataaccc gctgctgaaa gaaattgcgg caaaatataa
     7981 taaatccgtc ccgcaacttt gcatccgttt tagtttgcaa aataatgttc tgccgcttcc
     8041 gaaagcctcc tctcccgaac gaatggctca gaatctggac gttttcgatt ttgagatttc
     8101 ggaagaagat atggaaaaaa taatggcgat gcccgttacg gggtggtcgg gagaacatcc
     8161 ggacgccgaa ccggttgcga tttaatcatt cgatcaagag atgattttac ctgccagtga
     8221 aatgccgtta aaatcagtca cctctacgtt gctgatttgg ttcaataagt tttgactgcc
     8281 caaacatttc accggcaggt aattttcact gaatcccttc aatcggcaat tttctttgtt
     8341 gatcggttcg gcagactcct aaagcacatt tgtggttttc ccgatcattt tcatgccgaa
     8401 agattttctt tgttcatcca attgttcccg caatattttc gatcgttccc gtttcacatc
     8461 tttgggaatt cttccgtcta atttcgccgc cgcggttccc ggtctgacag aataggtaaa
     8521 cacatgcccg ccgctgaaag ccgccgtccg gacaaaatca agcgtccgtc gaaagtcttc
     8581 atcggtttct ccggggaaac cgacgatgat atcggtggtc acggaaaaaa gaggattgat
     8641 ttgtcttaca cattgaatca gccgtaaaaa atcaggtaaa ctgtttcggc gtgccattcg
     8701 tttcagtacc gcttccgagc ccgattgcaa cggcaaatgg agatgaggac acaagcgttt
     8761 attttcccat agacttataa atgcttcatc cagattccat gattccaaag aggataaccg
     8821 aattcgcgga atcgaagttt ccgccagcag tcgattcaat aaatctcgta acccttgcgg
     8881 tggattaaaa tccattcccc atgctccgag attcacaccg gtcaaaacaa tttcttttcc
     8941 gccgccgttt tccgccgcac gaatatcctg taaaatcgtt tcagccgcca cactacgcga
     9001 tttgcctcga gccaatcgcg taaggcagaa tgtgcaataa ttgtcgcatc cgtcctgaac
     9061 ttttataaaa gcccggttcc gaaaatgctc accgggtaag atttcacgct taatcgaaac
     9121 aggatttata aacggttccg gtattccggt gacaattgag gcaatctgtt ctttttcgtg
     9181 atttcgaatg acctgtgtta ctcccggcat ttttgaggcg gcttccggtt ccgtctccga
     9241 ccaacaaccg gtgacgatta cttgagtatg tccggatcga gcggcatttc gaatttttcc
     9301 tctcgaatcc gaagccgctt ctttcgtcac cgaacaggta ttgataatcg ctaaatcggc
     9361 ttcgtcggca ctgtccgcaa tttcgtaacc gtattgtttg aattttgccg caaattgttc
     9421 caattcggct tgattcaagc gacagccgac agaatccaaa aaaactttca atgacttccc
     9481 tcggaacctt tgcccagctc aattgtccgt tcgtagcacg cccacattgc ttcggcaatc
     9541 gcattgcgaa aacccaattt ttccaattcg aaaacggctt cggctgtcgt cccgcccggt
     9601 gaggtgactt catttcgtaa tacagccggg tgctcttttc gcatggcaag ataatcggtc
     9661 gaccctttga cggtccgaat aatgatttca gtcgccaaag gtcgcgataa ccccagatga
     9721 accgccgtat cgatcatcgc ttcgataaac agaaatacat aagccggacc cgaacccgaa
     9781 accgctgtta tttgatccag ttggtcttcg gatttcaact caaaggcata tcccgtacaa
     9841 gcgaggattt cttgtgccag attccgttga tcggcagcca cttcacgaga cgcaaaccat
     9901 ccggaaaccc cttcgccgat ttgtgccggc gtgttgggca tcacacggac aacccgctcg
     9961 tgatccagtc ccttttggaa ctgttcaatc gggacacccg caacaatgga tatcaccagc
    10021 gcatccggat gaattttatt tttcaattcg ccaaacactt cggaaagaaa ttgcggtttt
    10081 accgcgatca aaatcacatc cgcgtcacgg atcgcgtctg tcccgctgtc tgaaggattg
    10141 actcccaatt cctcataagc agaccgcatt ttgtcggcat cggtatcata aacagaaata
    10201 ttttgcggag agatcagccc ttttttcaag atacctgtca atattgcttt tcccatcacc
    10261 ccgataccga taattgccat tttttcggaa ttcatttttt atccctcctt ctgccaccgg
    10321 ctgtaaaccg ccattgacag taacctacct ccggacttgt cctttaaaca tacttctccg
    10381 gcggaaacac tgccacccat ccctttcatt acttcattga ccgtattata aactgttacg
    10441 gaagaagcct tgacggcata agcggtaacc aacaaaaaca aagcctcgga atccaaaata
    10501 tttcgacagg cttgaatcaa ttccggaaag ttcttataaa attcccaaac ttcccctttg
    10561 ggtccccgtc cgaatttcgg cggatccaaa atgattcccc ggtaaaagga accgcgccgt
    10621 gcttctctct gaacaaactt taacccgtca tcgataatcc aacgaatggg tgtttgttcc
    10681 aaagacgaca atttttgatt ttcctttgcc caatccacag cttttttgga ggcgtccaga
    10741 tgagttattt ccgcccccgc ctcggctgcg gccatggtaa tcgcaccggt atagccaaag
    10801 agatttaata ctttaaccgg tttctccgaa tttcggatta gggaagacac ccaatcccat
    10861 tggcaggctt gttccggaaa caccccaacc tgttttgagt tggataacat cagctgaaat
    10921 ttgagatttt tataaccgat attccaagga tgaatatctt tctcataaaa aatccaatgg
    10981 ccgccgtttt cctcaggaga aggtttaaat tcagcagaag ctttattcca ttccgtttcg
    11041 ggcagtaacg ggctccacat agcttccgct tccgggcgaa tcagaatata tctgccgaat
    11101 ctttccagtt ttcgtcctgc tccgctgtcc aaaagcatat aatctttcca atccggagat
    11161 tccaaaagct tcacttcggg taaatattta ttattcatag cgtcttcaag tatacttaga
    11221 tttgtcggat ttttgaaccg aaacaggaat ttgcggaatg atagggaaaa aatccattta
    11281 aattcaaaat aattcgaaat cgggaccggc atctgataaa cacggaaaga aaaggggttc
    11341 atgacgaaaa ttatcgaagt cgacaaactg tcaaaatctt acggccccgt caaagctgta
    11401 caagatatca gtttttatgt tgaaaaaggg gatctttttg cttttctcgg gccgaacggt
    11461 gccggaaaat caacgacaat tgatatcatt acaaccgttc tcatgcccga taagggaaca
    11521 gtaatcgtgg atggtttggt actgggaaaa gaggatgata agatccggtc ttcgatcgga
    11581 gtcgtttatc aggataatct tttggatcgt ttattgacgg tggaagaaaa tctcttcttc
    11641 cgagccggtt tctacggatt caataaagat cggatcagag agtccgtaaa gaatgccatt
    11701 ttaactaccg gaattcaaga ttatgcgaaa caacgttatg ggaaaatttc cggaggtcag
    11761 cgacgcagag cggatatcac acgcgctctg attaacacac cccaaattct gtttctggat
    11821 gaaccgacca ccgggctgga tccgcaaacc cgtaagaatt tatgggaagt tatccgaaaa
    11881 ttacaacgcg accgcgaaat gacaatcttt ttaacaacgc attatatgga agaagcggct
    11941 gaagcggatt atgtcgtcgt gattgacgag ggaaagattg ccgctcgcgg aactccgttt
    12001 gaattaaaag agaagtatac gagtgatctt ctaaaactat cctttaaaga cgaaataaaa
    12061 gggttgtccg acttggcaga ttggggaata acatacacaa aagcagggga tcagtttatt
    12121 atccgactgc cgcatactac agatgcgctt cccattttag accgctgcag agaaaacctg
    12181 aacggattcg aagttctgaa cgggacaatg gacgatgcat ttatcggaat aaccggaaag
    12241 gaaattcggg aatgatcgca cttgcatcca gaaatatcaa attatttttc cgtgataagg
    12301 caatggtttt cttttcgttg ctatcggtct ttatcattat cggtctttat gtgttgttcc
    12361 tcggcaatgt ctggaccagc gaatttccca atcaagaata tgcaaaattc atcatgaaca
    12421 gttggattat ggccggattg atatccgtca cagggtttac aacagccgtc ggcgccttcg
    12481 gaattatgat ccaagatcgt gaactaaata tagaaaagga ttttctttcc gctccggtca
    12541 aacgcagcag cttggcagcg ggttatctgt taagcgccta cggaatcagt gtaatcatga
    12601 gtctgattgc ttttattctc ggggaaatct ttattcttgc cgaaggcgga gaacttttag
    12661 gcatagttgc gatattcaaa ataatcggga tcattttgat taactcattc atgagtgttt
    12721 caatcattca ggttattatc ggattcattc atacgggaaa tgccttttct gcattcagca
    12781 caatcatcgg tacattgatc ggttttttga cgggaattta tctcccgatc ggacaacttc
    12841 cgaccggtgt acagaccctt attcgcattt ttcccgtttc ccattccgct tcacttgccc
    12901 gtcaaatttt tatggatgtt cctctgaaaa tcggtttcaa cggtgtcccc gaagcagccg
    12961 tcaatggttt caaagagttg atgggagtca ctttttttgt cggaagcaaa gaaatcacac
    13021 catggatgag tttatgctat atgctgctta tcggagccct tttctttgcg attgccgtta
    13081 tgaatctctc aaaaagaaaa ctggaataag caaagaattc ctctctgatc gtaaggagct
    13141 gagataattt ctctcctgcg gattcgggcg gttcttttat tcaaaggaaa atacacctcg
    13201 cagtactgag aacatttccg gcgatggatt aattttcgga ttgagaaaat atttgatccg
    13261 aattgaaccg attaaaacaa tctctcaagc gatttcttta agtcgaattt cactcgatct
    13321 ttatcgatcg ataaaaattc acctttttca taaagagttc ttccgtctac cattgtcatg
    13381 attacatccg cagcttgtgc ggaatagaca atcaaagaag gataatcatg aatcggcatc
    13441 agatggggtt tgtcaagatc aatcactgct aaatcggctt tctttcccac ttccaattct
    13501 ccacaattcg aacgtccttg tgcagctgca ccgttcaaag tcgccatcgt aaggacttca
    13561 tttggagaca aaacggtcgg atcctgagaa aaaccgcaat gaattaatgc ggttaaagcc
    13621 atttcttcga tcatattttg gttgttgtta ctggcggaag aatccgttcc gatcgttata
    13681 cgcagtcctt tttctttcat cttccgaatc ggcataacac cgcttcccaa tttcatatta
    13741 ctggaaggat tgtgaacaac cgtcacatct ttttccttca gtaagagaat atcttccggc
    13801 tccaccatca cacaatgtgc ggcgatcgtc ggattgtcaa acgttcccag atcatagaac
    13861 caggcagccg gagtttttcc ataacgtttc ttgcattctt catgttcttt tttggtttcg
    13921 gaaagatgaa tatgcatccg accgtttttt cccctacata tttccgaata ctcgcggacg
    13981 atctcgacat tgttggtata ctcggcatga atcgcaaaat ccgtcataat tcttccgtcc
    14041 gcagaaccat tatattcatc aaagaattca actgacagtc gcataatctc tttattttta
    14101 tccgaatcgg aagccataac cgccataatc ggaaagctca gatttgcttt cataccggat
    14161 tgaatcacat cctcgatata atggttcgga taatggtaca tatcagaaaa agagaccact
    14221 ccggaagcca acatttccat catttccaac ctgaccccga cgctcttatc cgccggcgtc
    14281 attttttgtt cgatcggaag catggcttca aataaccaac ggtccaacgg taaattagtc
    14341 cctaacccac gcaaagcagc catgggggta tgagtgtgca tgttgtataa gcccggaata
    14401 atcagacaat tgtaaaaatt tctctcaaca tcatattctt cgatcggttt tgtctcgccg
    14461 atataagaga ttatctcatt tgtaatcccc aaataaccat cccgaatgac accctggcta
    14521 tattttggtg ttaagatatc aacattcgaa aataaggttt tcatgatttt tctctcccga
    14581 atttttccgt tcaacttgct ttgttgatta aaaagtttaa gcagttcatt tacacgatgg
    14641 aaatcaaaac aaaaagtagc gagttattgg atatatcatc ccatcgctaa ccgaatttct
    14701 gaaattatac ctgtgaaaac ggaaaacaaa tgccgaaagg aggtattttt aaaattattt
    14761 tatataaatt taattattga ttatcgtact atattttagt atatgtttga tctaataacc
    14821 atgaatccat tgagaaattt ttcattatgt gagaattact catatattta atattttatt
    14881 ttttattatt actttcaact taattcgata gcaatttgta ctatatttca cataattacc
    14941 caaaattaat tgacgtttcc ttattattaa cctatcattt aattattagc gttggctata
    15001 aataaataga atataggttg attgttcgta atagaaggag attttatgga aaaaaagact
    15061 ctaatagtta atggaatccg ccaagtcgtt attcataatc cggatgcgat gttagcggat
    15121 gtgcttcgaa accagctgca tgttctggga gtaaaaaaag gttgcggtca gggacactgt
    15181 ggcgcctgtt caatcatatt gaacggcaaa cttgttttat cttgtatcca taaaatgtcc
    15241 cgaattccgg atgaatcaac catattgact gttgaaggga tcggtacacc cggacatctg
    15301 cacccaattc aaacagcaat gaaattccac agcgctgcgc agtgcggttt ttgtactccg
    15361 ggttttgtga tttcttccta tgcattgcta caggaaaatc cgaatccgac tcgacaacaa
    15421 attcgggatt ggtttacaga acatcataat gtttgtcggt gcaacggcta taaaccttat
    15481 attgattcag tgatcgatgc cgcagccgta ttgcgcggag aaaaaagtat tgaagaaata
    15541 acatataaag aacccaaaga caataaggtt tggggaactc gtcgtcctcg tccgtccttg
    15601 gatgcaaaag tgaccggcac atgggatttc ggtgcggatg tggcagcaaa aatgccttcg
    15661 aatacccttc attgtgcctt ggttcagtca caggtacctc atgcgaatat tatctctgtt
    15721 gacacttccg aagctgaaaa agcggaaggc gttttcaaag tggtaacggc gaaagatgtc
    15781 aaaggtcgaa accaaattac cggcctgatc accttcccga caaataaagg agacggatgg
    15841 gatcgtccga ttctctgcga taaaaaagtt ttccaaatcg gagacgcgat cgcgattgtc
    15901 tgtgcagata cccaggaaca ggcggatgcc gcggcaaaac tggttaaagt cgaattggaa
    15961 attttgccgg cttacatgaa cgctccggcc gcaatggcag aagatgccat tgaaattcat
    16021 ccggggactc cgaatattta ttttgaacag aagaatgtaa aaggaaacga taccaaacct
    16081 atttttgaaa aagctgctta tacggtcgaa gggaattatt acctgcaacg ccagccgcat
    16141 ctgacggtgg aaccggacaa tgggtttgct tacatagacg aagacggcaa tctgactatt
    16201 cattcaaaat ccatcgggat tcatctccat gcggcaatga ttgcggaagg gatgggtgtt
    16261 ccgctggata aactgcgaat tgtagaaaat aatgccggcg gcactttcgg atataagttc
    16321 agcccgacaa tggaggctct ggtcggagtc gcttgtctgg ctacacaacg tccggtcgtt
    16381 ttgatctaca actattatca acatatgatt tataccggaa aacgctctcc catgttcctg
    16441 aaatgcagaa tggcagccga tgaaaacggc aaattattag ccatggaagg ggattggtct
    16501 gttgatcatg gtccttattc cgaattcggt gatctgctga ccttgcgcgt cagccaattt
    16561 atgggcgccg gatataatat tccgaacatc cgaagtatcg gaagaacagt ttgtaccaat
    16621 catatatggg gttccgcctt ccgcgcttac ggctcgcctc aagctttttt ggcttcggaa
    16681 tcttgtatcg atgaactcgc cgaaaaaatg gggatcgatc cgttcgaact gcgttatcga
    16741 aatatttatc gtgaaggatc aactacgccg accggtcaaa agcccgacag tttatgcttc
    16801 gaagagatgt tcgatacgat gagaccgaaa tatgaagcag ctaaagctaa agccaaagcc
    16861 gaatcgacac cggaatataa gaaaggcgtc ggtatttcat tgggggtcta cggttgtgga
    16921 ttggacgggg ctgattcttc ggaagccatt gctgaattat ccccggacgg aaaagtcatt
    16981 atctattctt cctgggaaga gcatgggcag ggaaatgatg cgggtgcctt gggtaccgct
    17041 catgaagccc ttcggaaagc cggcttcaaa cccgaacaga ttaaattggt attcaacgac
    17101 accaaaataa cccctaattc gggacctgcc ggcggttcac gctcccagac catgaccgga
    17161 aatgccattc gaatcgcctg cgaaaatcta cttgaccaaa tgaggaaggc ggatggcagt
    17221 tatcgtactt atgatgaaat gaaagcggac ggcaaggaaa ccaaagtaac cggaaaattt
    17281 gtcatgtccg aagccaccag ctgcgacgaa aacggtcaag gtcgcccctt tatggtttac
    17341 cagtacggtt tattcatggc ggaagtgacg gtagaaatag caaccggaaa aaccaaagtc
    17401 gataaattta cctgcttcgg cgattgcggt gtcatcaaca accgtgcgat tgttgaaggc
    17461 caaatgctgg gtgggtgtgc tcagggaatc ggcttggcgc tgtcggaaga tattgaagac
    17521 tataacaagc atactaccat gcgtggtgca ggaattccgt actgcaacga tatccccgat
    17581 gagtgggata tcgaaatgtt cgaaaatccg cgcgaatatg gtcctttcgg cgccggcgga
    17641 gttggtgaac ttccgctgac cgcttcccat gttgcggtta tcaacgctat aaataatgcc
    17701 tgcggtgtgc gaattcgcca ccttccggct tacccggaaa aggtgttgga agggttgaaa
    17761 gcgctgaaca agtaaaaagt ctgattttat gcaaaaggct gtccttctcc tccaacaggg
    17821 cagcctttta tttttgagaa gatatgattc atttcaattt ttcaagctac aataacaata
    17881 ccaaggagta aaaatggatc agcaattcat gcgcacaatg gaatccatat gcatccagaa
    17941 tgagccgcct gcttgtgtga ccgcttgtcc tttacatgtc gacagccgga cgatgttaaa
    18001 actggttgct tccggaaaat ttgaggacgc atattcactc tatcagaaaa atgtcccgtt
    18061 cccttcgatt ttaagcaaaa tttgtaattc cccttgcctt tcaaaatgcc gtctttctga
    18121 aatcggtgag ggaatacaaa tccgtccgat cgaagctttt ctgaatcagt atgccgatcc
    18181 gtctttttta ccgaaggtat tgccggaaaa acgccagaaa gcgggaattt tcggatcgga
    18241 attggacagc ttatccgccg catatgattt acgcaggaaa ggttaccaag tgacgattct
    18301 ggatgcggat gccgttttag ccaaaaattt gcgtgcgttt aacgaagaag ttttaccttc
    18361 cgttgaaata gaaaatgcaa ttcatttgct cgaaaaaatc ggtgtaaaaa tacaaaacga
    18421 tttcgaaata ccccgttttg aagaattatc tcggtatgat tcgggctttt tccgaatcgg
    18481 cgattcggaa tttcaatgca tttatcttcc ccctttgtcc ggagaacttt caattgattc
    18541 gataacccgt atgacggtta ccgaaggggt cttcagcgga agcgcatcgg ccggctactg
    18601 ggtcgatcaa atgtccgacg ggcggagagc ggcgatttct atggatcgtt atctgcaaaa
    18661 agtatccttg acggcttcca gagaaaacga aggagcctat gaatgccgcc tgttcacgtc
    18721 tctaaaaggc attcaacctt ccgaatcgtt catttctacg gacataaaaa gaaccggcca
    18781 ggcggatatc ataaaagaag ccgcccgttg tatccagtgt caatgtttgg aatgcgcaaa
    18841 aggatgtgaa tttattcgac attaccaaga ttcgccccga acttatgtca ggcagattta
    18901 taacaatatt tctatttgta ccggattgcg tcagaaaaat aaaatgataa actcctgctc
    18961 gatatgcggc caatgtgaag tcatatgccc gaaccatatt ccctttcaaa aagtcgtccg
    19021 agaggctcgt gaaacaatgg tggcaacgaa gaaaatgccg ccgtccgctt tcga
//
LOCUS       DAOMIV010000002        13742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_4426_length_13742_cov_5.094104, whole genome shotgun sequence.
ACCESSION   DAOMIV010000002 DAOMIV010000000
VERSION     DAOMIV010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 13742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13742
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4426_length_13742_cov_5.094104"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1177)
                     /locus_tag="PKV59_00070"
     CDS             complement(191..1177)
                     /locus_tag="PKV59_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQP45172.1"
                     /translation="MIQIAIIGLGEIGTSLGLALKYQKDEIYRVGMDVHKYATDNASE
                     KGAVDRVSRNLLDAVKEADIVFLAIPAHEVEDTLELIGHELKKDAAVLDTCPTKSAAT
                     NWAKKSMSQPDHFLGIYPSINPAYLSEPSNEFHSAHEDLFQSGTLYLAPSADTEPAVV
                     KLASDLGTLIGAHSAFIDPAELDGLLAAAYDLPLLLSNALMSTLLNQNSWEDCRKLAV
                     KEFVQETDFCAFPYTGKEFTEILSENRENTLRILNDFIVNLKTYRDAIQNRDRAALSS
                     LYEKAEKGREKWLKDYEEGAWRKEKDQKLNVPTAGEMMGQMFLGGLGRKKKE"
     gene            complement(1182..1805)
                     /locus_tag="PKV59_00075"
     CDS             complement(1182..1805)
                     /locus_tag="PKV59_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQP45173.1"
                     /translation="MAVPAVFVCRILTLIIAFTIHEFAHGFLADRFGDPTPRLQGRLT
                     LNPLKHLDIFGSLMLLLTGFGWAKPVQIDTEAIRRNNKAGVMLVSFIGPFSNFLLALF
                     GALLLKIPGITTIRWWGIPFFLSQFVWINLSLLIFNLVPLAPLDGEKVVEYFIPDSML
                     PAWKNVQQYGMQILLICFLILPYLNIDVFGKILSPLISGLYYWLVGG"
     gene            1884..2108
                     /locus_tag="PKV59_00080"
     CDS             1884..2108
                     /locus_tag="PKV59_00080"
                     /inference="COORDINATES: protein motif:HMM:NF017879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HQP45174.1"
                     /translation="MDEEYELGDLITLKKMHPCGSNQWVVVRLGAEIGLECLNCHHRV
                     LLTRRELSHKMKGKPQKNPERPSESSEPST"
     gene            2169..2663
                     /gene="tsaE"
                     /locus_tag="PKV59_00085"
     CDS             2169..2663
                     /gene="tsaE"
                     /locus_tag="PKV59_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQP45175.1"
                     /translation="MPILDSETLEIFSRSADQTRRFGMRLGRMLQNGDVLCFSGDLGS
                     GKTTFIQGIAQGWGSVDQVTSPTFVLVNEYYRMDGSTLFHMDAYRIDSTLEAEELDID
                     RMLHQGVLVVEWAERIDSVLPKERLTIKMAWVADEQRSLVMVPYGERYQTLLHSFRHR
                     SFGV"
     gene            2670..3338
                     /gene="tsaB"
                     /locus_tag="PKV59_00090"
     CDS             2670..3338
                     /gene="tsaB"
                     /locus_tag="PKV59_00090"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQP45176.1"
                     /translation="MLLAIDTSTSNIGIAVYDGNSIIGEANWLSLNRHTVILAASVKN
                     LFDTVDTDISKLRGIAVAMGPGSFTSLRVGLSFAKGMALGLDIPVIGVPTLDILAAAQ
                     PINGLPLCAVLQAGRTKLAAAFYNPSKDKWAESLPVSVYTTESLCEAITEPTTVCGEL
                     TADMRTYFRRNNKNIRMASPAMCLRRAGFLAEIAWARLEKGDYPPPNTLSPIYLHTKE
                     PISQ"
     gene            3341..3859
                     /gene="rimI"
                     /locus_tag="PKV59_00095"
     CDS             3341..3859
                     /gene="rimI"
                     /locus_tag="PKV59_00095"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQP45177.1"
                     /translation="MIGRTDISIRRMVLTDIPAVFTIDQASAALYWPQSSYYFEVERN
                     EASRPWVAVDSQQRVCGFLVLWLIMDELHVANFAVHPDYRRRGIGDALMQNGLYEGWK
                     EGARISYLEVRTGNSAAISLYEKIGYRVVNIRRHYYQDNQEDALMMNLEMQDYQTYIL
                     TLEKLWMIPTKK"
     gene            3835..4494
                     /locus_tag="PKV59_00100"
     CDS             3835..4494
                     /locus_tag="PKV59_00100"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQP45178.1"
                     /translation="MDDSYEKMTQLAQQIAVCEKCQLHLSRKKSVPGEGNIHSKILFI
                     GEGPGLNENETGRPFVGQAGNFLNELLSTAKLKRQDVFITNVVKCRPPANRDPLPEEL
                     TACSSFLDEQIRLIDPLVIVTLGRFSMGRYFPLARISSIHGKGHWVDGRMIVPMFHPA
                     AALHQPNLRDSIMHDFALLPTYIHEVEEKITAEPVENANPASEAAEKKEATDGVQLSL
                     F"
     gene            4560..5678
                     /locus_tag="PKV59_00105"
     CDS             4560..5678
                     /locus_tag="PKV59_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP45179.1"
                     /translation="MKVIFFANTDWYLYNFRLDFAKYLREKGWEVVLMAPAGEHTEKL
                     TAAGFRFIPFEFSRKGMNPFIEQRTIQRIKKIYAEEKPDLVHHFTIKCVIYGSLAAKE
                     VGIRAIVNSITGLGYIFLDNKITTKMIRKIVSSLYKKALKDTEVIFENPDDAALFEKY
                     GFTTEGNTHIILGTGIDTELFRPIPPPDSIPLIILPARMLWDKGVGEFVKAAEILKSK
                     SLQARFALVGKKDDGNPSSISYEQLSFWQKEGNVEWWGWQEDILTVISISDIVCLPSY
                     REGLPRILIEAASCGRPIVTTNVPGCREVVVDGVTGFLVPAKDPKSLAYALEKLIRSN
                     EMRIEMGTAARKRAIEFFSISRVNDEIFKIYQQLTQDS"
     gene            5675..6760
                     /locus_tag="PKV59_00110"
     CDS             5675..6760
                     /locus_tag="PKV59_00110"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQP45180.1"
                     /translation="MILPEISTGKIFETVFVSFFLCLIIVPIAIRFSHKTNLIDFPLS
                     EPHKIHKRPVPISGGLAFFATLILTAVFLNHRLDSQTGKVLLCATIIFGFALWDDYRP
                     LRWRWKMLGQILAAIVLYFCGIQVQIMQTVGEILGFSATVNDLLDFGITIFWMVFITN
                     AFNLVDSMDGLMVGLSLLALIFFVLAAMDTKQWSLALLCSAMLGACIVLNFFNSSPAI
                     LFFGDSGAQTIGFLIAAIAIIYNPPDRLQTSTWFMPILLLGVPIFDTVLVIISRFRRH
                     LHFYDSGTDHTYHRLVKMGFSDVQAVNLMLLAATALEILAFFIISRPPLTANLIFFAV
                     VLAGIFLIYFFESENMWESIRLKKNGD"
     gene            6724..9378
                     /locus_tag="PKV59_00115"
     CDS             6724..9378
                     /locus_tag="PKV59_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45181.1"
                     /translation="MGIDPSQEKRGLKPIPLFFSLLTVVSLICGYTLFKIPSDQKNAF
                     ILGLSLERVVMLAFFLLIALASLVGAIIFNINQQKNASLEASILACKEQIKRIASVTA
                     VLSGGLLLMPDYRFGRFSAYFHRLRPFLFLLFWVGFSAAMILIVFSDKNIFKRIKDDC
                     RQITGKPFFIFLGLFLLTILFIYGSGWGIVAGKEAWYGNAVPVESLQIVLIIVFLLFL
                     SPFMTKIGRVKLFRNRTFLFFLIWGIAAFTWSMAPMQAHFFAPGPYPPNYEYYPYSDA
                     LLNDVAAQTAVYGLKFYHGTLVLKPFVTFVIYLCTLLSGNQMNLTLLIQSAIFAILPA
                     ILFLFASDLGGLFSGFLAAALMIFQEWNALHTTQILTIHSRLEMSEFLAEVLFAAFAY
                     FIFKWFRQGRKQTYFAAAAGGVLGLLIYTRFNFVAMIPAIILFGGIVFHKKIKRGIRD
                     LLIFLLSFVICISPWVVRSYHITGTIAPEILDSFKSVVVEQRLKPITETSEISVENKE
                     PVQPTAEPVLQQHMEATPAIGKQIEKETETQLKSFSLIPDEIRLNVHPLIDTIGNHFF
                     HNLFSIAFILPVQIQFDDLNHLYTAEDSVWRDHWNGSLSAGQILFLIINLILISTGLN
                     LLWKKESWAGLSAAYLLIVYAAGLGLARTSGGRYIVPMNWGVLLIYSIGLAVILKKIT
                     PIRTDLVTGSTEISNQTIGIGDFGKLVLIIGIFFMAYFSMILIEKNTSPSIPSENKLI
                     VTEKMKETFPDQDWTQAETQLREGKMRLFQGVALYPRFYYFNQGEHGTDKAYAYQLYS
                     RIIFKILFDNGESDIVLPLDKFPDTFPDQSSVAVIGCLEPGSSYIKGIAVIGQTPDGK
                     EFHYLRSPMKEIACPIPEPVCPEVNKCY"
     gene            complement(9382..10515)
                     /locus_tag="PKV59_00120"
     CDS             complement(9382..10515)
                     /locus_tag="PKV59_00120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP45182.1"
                     /translation="MKILTVIHEFPPIGGGGGLIARDLACALVQDGNEVRVITAQFGD
                     LPDREVLDGVEIIRLKSHRKEAYRAKLLPMASFILAASRYCVYQMKDFSPDIIHAHFA
                     VPGGPPAKFISGKKHIPYVLTVHLGDIPDASPEKTGKWFKFIYPFTHTFWKHASAIVA
                     VSEFSKRMAQRKYPVEIQVIPNGIDYDQVRIERIEPHSVPEIVFAGRFVPQKNLEQLV
                     KVCAAIKDLNWHCTLIGDGQDREKIEELIERLNLQNKISITGWVAPERVTEIFKQSDI
                     LFLPSRSEGLPVSGIQAMTCGLALVLSNAGGNPEIIEEGKNGFSFDPEDTDSFASALR
                     ALLESPEMLRSFRQNSLESAKKYDIKRIAKQYEAVFEQVLAAK"
     gene            complement(10512..11465)
                     /locus_tag="PKV59_00125"
     CDS             complement(10512..11465)
                     /locus_tag="PKV59_00125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQP45183.1"
                     /translation="MYLSVIAPVYNEHDNIPLLYEEMVKALKDFDKEWEFILVDDGSV
                     DGSFEELTKVAEKDPAHVKVIGLRRNFGQTAAIAAGVDYSTGEIIILIDADLQNDPAD
                     IPMMLGKIDEGYDLVSGWRKSRQDNLSRKIPSVIANKIIGHVTGVPLHDYGCTLKAYR
                     REVITGFRLYGEMHRFIPAYANTVGARIIEVEVKHHPRRFGKPKYGLERTAKVILDLF
                     TVKFLSSYSDKPIYLFGGSGLFLTIIGGLLLLFLAVRRLGWGVSVLASPLFTFSIMIF
                     ILGFQLIMMGLLAEMLTRSYYESQGKRTYTVKKLINFKSDK"
     gene            complement(11493..13394)
                     /locus_tag="PKV59_00130"
     CDS             complement(11493..13394)
                     /locus_tag="PKV59_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQP45184.1"
                     /translation="MPLISAQKLSKSFGAEDIFSEITLSIPRGAKIGLVGPNGSGKST
                     LLQILMGITPSDSGSVQRAKKITIGYLPQNFEAEFEKSPYQECLSVFSDLILMKSKLN
                     QMEQDLANGNNVSELLSSYGNLQNTFESRGGYVFETRIHQVLQGLGFSKSEENRPWYQ
                     LSGGQKTRAYLAKILLMNPDLLILDEPTNHLDINAIEWLESFLKEYKGGALIVSHDRY
                     FLNQTVEVIWELDSSFEIYHGNYDAYLQQRQARYERQKAEFESQQEFIAKEEEYIRRN
                     IEGQNTRQAQGRRTRLERLLEESKITNPILHRKIQTNLSSSIRSGDIVLRTKNLEIGY
                     LDDHKKLISIPDVVLQRGECVAILGPNGVGKSTFIKTILNEIPPLAGEVEMGANLKVG
                     YFAQAHEKLNPKATLMDEISAAAPQMLPAEIRSYLARFLFTGDDVFKTTETLSGGERG
                     RLALAILALQNANLLLLDEPMNHLDLEAQETLQALLKDFNGTILLVSHDRYLVDAIAT
                     QIWEVNPDKKTLEIFRGNYSEYKEYQKKSGISELKDQIDSTERKSPEIRENPPLQKLS
                     NNEIFRRQKKKEELEKRISVLEKMLNEISQQMVSQKEDHQKIAELGIRYNSIEIELNS
                     VMEEWAEID"
BASE COUNT         3682 a         3094 c         2913 g         4053 t
ORIGIN      
        1 acgagggata cgggggaatg cattttggta taagacgcac cgtgcccgga attatgagct
       61 ttaagccgtc tttcgggatt cgtcgtccaa ccggtataaa aagatccgtt ggaacattcc
      121 accatataac aataaaacag accggtcccg gacgccgaca atgttgtttg cccgttaccc
      181 gtatcagcgg ttattctttc tttttccgcc ccaatccgcc cagaaacatc tgtcccatca
      241 tttcccccgc ggtcggtaca ttcagcttct gatctttttc tttccgccaa gcgccttcct
      301 cgtaatcttt gagccatttt tcccttcctt tttccgcttt ttcataaaga gagctcaaag
      361 ccgcacggtc gcggttttga atggcatccc gataggtttt caaatttacg ataaaatcat
      421 tcaggattcg cagcgtattt tctcgatttt ccgacagaat ttcggtgaat tcctttccgg
      481 tataagggaa agcacaaaaa tcggtctctt gtacaaattc tttcacggct aatttgcggc
      541 aatcttccca actgttttga ttgagcaacg tgctcattaa agcgttcgat aataacagcg
      601 gaagatcata agcagcagcc aataatccat ccaattcagc cggatctata aatgcgctgt
      661 gggcaccgat caacgttccc aaatccgacg ccagtttgac tacggcgggt tcggtatcgg
      721 cagacggagc caaatacaat gtaccggatt gaaacagatc ttcatgcgcg gaatgaaatt
      781 cattcgacgg ttcggagagg taagccggat tgatcgatgg atatattccc aaaaaatggt
      841 ccggttggga catcgatttt ttcgcccagt tggtcgcagc cgattttgtc gggcaggtat
      901 ccaaaaccgc ggcatctttc ttaagctcat gacctattaa ttcgagtgtg tcttccactt
      961 catgggcggg aattgccaaa aaaacgatat ccgcttcttt tactgcgtcc aataagtttc
     1021 gggaaactcg gtcgaccgct cccttctccg atgcattatc cgtcgcatat ttatgaacat
     1081 ccatcccgac ccgatatatt tcatccttct gatactttaa agcgagcccc aacgacgtgc
     1141 cgatttctcc cagcccgatg atggcgattt gaatcatagt tttatcctcc gaccaaccag
     1201 taatacagtc cgctgatcaa cggacttaag atttttccga aaacatcgat attcaggtac
     1261 ggcaatatca aaaaacagat caataaaatt tgcattccgt attgctgtac attcttccag
     1321 gcaggaagca tactgtccgg tatgaaatat tcgaccactt tttccccgtc caaaggagcg
     1381 agaggcacca gattgaaaat cagcaggctg agattaatcc agacgaattg tgaaagaaag
     1441 aaaggaattc cccaccaccg gatggttgta ataccgggaa ttttcaacag caacgcaccg
     1501 aataaagcca acagaaaatt ggaaaaagga ccgataaaag agaccaacat cacaccggct
     1561 ttattgtttc tgcgaatggc ttccgtatcg atttgaaccg gttttgccca cccgaaaccg
     1621 gttaataaca acatcaaaga gccgaaaata tccaaatgct tcaaaggatt caaagttaac
     1681 cgaccctgca gccgcggagt cgggtctccg aaccgatcag ccaaaaaccc atgcgcaaat
     1741 tcatggattg tgaatgcgat gattaaggtc aggatgcgac atacaaaaac tgcgggcacg
     1801 gccatgcagc tattataaca tttgcatccc gaaccgttat tgtgactctg ttcagcccct
     1861 ttcaattcct ttataattgc aggatggatg aggagtacga gcttggcgat ttgatcacat
     1921 taaaaaaaat gcatccatgc ggttccaatc agtgggtagt ggttcggctt ggcgccgaga
     1981 ttgggctgga atgtttaaat tgtcatcatc gcgtcttgct gaccagaaga gaattatcgc
     2041 ataaaatgaa ggggaaaccg caaaagaatc ccgaacgtcc atctgagtct tcggaacctt
     2101 caacataaaa caaattcgag caaatctgat acttttcttc ccaacctgat atcttaaagg
     2161 atccgtttgt gcctattctt gattcggaaa cattggaaat tttcagcaga agcgccgacc
     2221 aaacccggcg gtttgggatg cgtctgggaa gaatgttaca aaacggtgat gttttgtgct
     2281 tcagcggaga tttgggatcg gggaaaacca cttttatcca aggaatcgca cagggatggg
     2341 gcagtgtcga tcaggtaaca agcccgactt ttgtcctggt aaatgagtat taccgaatgg
     2401 acggaagcac actttttcat atggacgcat atcgtatcga cagtaccctt gaagcggaag
     2461 agctcgatat cgaccgcatg ctgcatcaag gcgtgttagt cgtcgaatgg gcggaaagaa
     2521 ttgattccgt tcttccgaaa gaaagattga caatcaaaat ggcttgggtg gccgatgaac
     2581 aacgcagcct ggtcatggtt ccatacgggg aacgttatca aaccttattg cattcatttc
     2641 gccaccggtc tttcggagtt taaataaata tgttattagc aattgacact tctaccagta
     2701 atatcggaat cgccgtttat gacggaaaca gtatcatcgg agaggcaaat tggctgagtc
     2761 tgaatcggca taccgtcatt cttgccgctt ccgtcaaaaa tcttttcgat acggttgata
     2821 cggatatctc aaaactccgc ggcatagcgg ttgcaatggg tcccggatcg ttcaccagcc
     2881 tgcgtgtcgg cctttcattt gcaaaaggaa tggcgctggg cttggatatt ccggtcatcg
     2941 gtgtcccgac tttggatatt ctcgcagccg ctcagccgat aaacggattg ccgctctgtg
     3001 ctgtcttaca ggcaggcaga acaaagcttg ctgcagcttt ttacaatcct tcgaaagata
     3061 aatgggcgga aagtttaccg gtttctgtct atacgacaga aagtctgtgc gaagcgatta
     3121 cggaaccgac aacggtctgc ggagaattga ccgcagatat gcgtacctat tttcgcagaa
     3181 acaataagaa tattcgaatg gcatcaccgg caatgtgttt acgaagggcg ggttttttag
     3241 ccgaaattgc ttgggctcgt cttgaaaaag gagattatcc tcctccaaac accctgtctc
     3301 cgatctatct tcatacgaaa gagccgattt cgcaataaat atgatcgggc gcactgacat
     3361 ttccattcgc agaatggttt taacggatat cccggcagta ttcacaatcg accaagcttc
     3421 ggctgctctg tattggccgc aaagttctta ttattttgaa gtcgaaagga acgaggcatc
     3481 acgcccctgg gtagcggtcg actctcagca acgtgtctgc ggatttcttg tcctttggct
     3541 tataatggac gagctgcatg tcgcgaattt tgccgttcat cccgattacc gccgacgggg
     3601 aatcggcgat gcattaatgc aaaacggatt gtatgaaggt tggaaagaag gggcaaggat
     3661 ctcttacctt gaagtccgaa ccggtaattc agccgcaata tccttatatg aaaagatcgg
     3721 ttatcgggtc gtcaatattc gaagacatta ctatcaggac aatcaagaag atgctctgat
     3781 gatgaatctg gaaatgcaag attatcaaac ttatatcctg acattggaga agttatggat
     3841 gattcctacg aaaaaatgac tcagcttgca caacaaatcg ccgtttgcga aaagtgtcaa
     3901 cttcatttat ccagaaaaaa atccgtcccc ggagaaggga atattcattc caaaatcctt
     3961 ttcatcgggg agggccccgg tttgaatgaa aatgaaaccg gcagaccttt tgtcggccaa
     4021 gccggtaatt ttctcaacga actgctttcg acggccaaac tgaaacgaca ggatgttttt
     4081 atcacaaacg ttgttaaatg cagacccccg gctaatcgtg atccgctgcc cgaagaattg
     4141 accgcctgct cttccttttt ggatgaacaa attcggctga ttgacccgtt ggtcatcgtt
     4201 actttaggga ggttttcgat ggggcgctat tttccgcttg cgagaatcag ttcgatccat
     4261 gggaagggcc attgggtgga cgggcggatg atcgttccga tgtttcatcc ggcagctgca
     4321 ttgcatcaac cgaatcttcg ggacagtatt atgcatgatt ttgccctttt accgacctat
     4381 attcatgaag tggaagagaa aattacagcg gaaccggttg aaaatgccaa tccggcctct
     4441 gaagcagccg agaaaaaaga agcgacagac ggcgttcagc tttcgctctt ttagccggct
     4501 cgtaaagatc aatccctctg ctttataatt agttcagatt aaataaaagg atcaaaccct
     4561 tgaaagttat ttttttcgcc aatacagact ggtacctcta taattttcga ctggattttg
     4621 cgaaatatct ccgtgaaaaa gggtgggaag tcgtattaat ggcaccggcg ggggagcata
     4681 ccgaaaaact gacagcagcc ggttttcgat ttattccttt tgaattctca cggaaaggga
     4741 tgaatccgtt tatcgaacag cgaaccattc agcgaataaa gaaaatttac gcggaagaaa
     4801 agccggatct ggttcatcat tttacgatta aatgtgttat atacggatct cttgccgcca
     4861 aagaggtcgg gattcgagcg attgtcaatt ccatcaccgg cttaggctat atctttttag
     4921 ataacaaaat taccaccaag atgatccgga aaatcgtgtc ctctttgtat aagaaagcgt
     4981 taaaagatac cgaggtcatt tttgaaaacc cggatgatgc cgctctcttc gaaaaatacg
     5041 gatttacgac ggagggaaac acccatatta ttttgggaac aggtatcgat accgagttgt
     5101 ttcgtccgat tcctccaccg gattccattc ctctcatcat tttacccgcc aggatgctct
     5161 gggataaggg agtaggagaa tttgtcaaag ccgcggaaat attaaagagc aaatcacttc
     5221 aagcacgatt cgctctggtc gggaaaaaag atgacggcaa tcccagcagc atttcttatg
     5281 aacagctgtc cttttggcaa aaagaaggaa acgtagaatg gtggggatgg caggaagata
     5341 ttctgaccgt tatttccata tcggacattg tatgtctgcc atcttatcga gaagggttgc
     5401 ccagaatatt aattgaggcg gcttcttgcg gacgtcccat tgtcaccact aatgtccccg
     5461 gatgccggga agttgtcgta gacggcgtca ccggatttct ggttcccgca aaagatccga
     5521 aatcattggc ttatgctttg gagaaattga ttcgaagtaa tgaaatgcga atcgaaatgg
     5581 ggaccgcggc aagaaaaaga gcaattgagt ttttctcgat ctctcgagtg aacgacgaaa
     5641 ttttcaaaat ctatcaacaa ttgacacaag actcatgatt ctgccagaga tttcaaccgg
     5701 aaaaatattt gaaacagttt ttgtcagttt ctttttatgt ctgattatcg ttccgattgc
     5761 aattcgtttc tcgcataaaa cgaatttaat cgatttcccc ttatcggaac cccataagat
     5821 tcacaaaaga cctgttccga tcagcggcgg attggctttt tttgcgacac tgattctcac
     5881 cgctgtcttc ctgaatcacc gtttggacag ccaaaccgga aaagtgctcc tttgtgccac
     5941 aatcattttt ggattcgcac tgtgggatga ttacaggcct ctgcgttggc gatggaaaat
     6001 gctcggccag attttggccg cgatcgtgct ctatttctgc gggattcaag tgcagataat
     6061 gcaaaccgtg ggtgaaatat tgggtttttc tgcaacggta aatgatcttc tggattttgg
     6121 aattacgatt ttctggatgg tttttattac caatgctttt aaccttgtcg acagtatgga
     6181 tgggttgatg gtagggctct ctttgctggc gcttatcttt tttgtgttgg ctgccatgga
     6241 tacgaaacaa tggtcgcttg ctctgttatg ttccgccatg ttgggagcct gcattgtgct
     6301 gaattttttc aattcgtcgc ctgccattct gtttttcggc gattccggag cgcagacaat
     6361 cggttttctg attgcggcaa tcgccatcat ttataatccg ccggatcgct tacagacttc
     6421 cacatggttt atgccaattc ttctgttagg cgtgccgatt tttgacacgg tattagtaat
     6481 tatttcccgc ttccgcaggc atttgcattt ttatgacagc gggacagatc atacctatca
     6541 ccggctcgta aaaatgggtt tttccgatgt tcaggcagtc aatttaatgc ttttggctgc
     6601 gacagccctt gaaatacttg ctttcttcat catttcacgc ccgccgttaa ccgcgaatct
     6661 gattttcttt gccgtcgttt tggccggtat attcttgatt tacttttttg aaagtgagaa
     6721 catgtgggaa tcgatccgtc tcaagaaaaa cggggattaa aacccatccc tttgtttttt
     6781 tccctgctga ctgtcgttag tttgatatgc ggctatactc tatttaaaat tccttcggat
     6841 caaaaaaatg cctttatcct cggactttct ctcgaaagag ttgtcatgtt ggcatttttc
     6901 cttctcattg ctctggcatc cttggtcggt gcaatcattt tcaacataaa tcaacaaaaa
     6961 aatgcatcct tggaagcgtc tattcttgct tgtaaggagc agatcaaacg aatcgcttcc
     7021 gttacggcgg ttctttcggg tggcttgctg ttaatgccgg attatcggtt cggacgattt
     7081 tctgcctact ttcaccgcct gcgtcctttt ctgtttttgc tgttttgggt cggtttttcc
     7141 gccgctatga tcttgattgt cttttcggac aagaacatat tcaaaagaat taaggacgat
     7201 tgccggcaaa ttacggggaa gccctttttt atttttctcg gcctttttct attgaccatt
     7261 ttgtttattt acggatcggg atggggaatc gttgccggga aggaagcttg gtacggaaat
     7321 gcggtccctg tcgaatccct tcagattgtc ttaataatcg tttttttatt gtttctttct
     7381 ccgttcatga caaaaatcgg acgggtgaag ttatttcgga atcgtacttt cctctttttc
     7441 ttgatatggg gtatcgccgc cttcacttgg agcatggcgc cgatgcaagc tcatttcttt
     7501 gcaccgggac cttatccgcc caattacgaa tattacccgt attcggatgc attattgaat
     7561 gatgtggcgg ctcaaaccgc cgtctatggc ttaaagtttt atcacggcac acttgtgttg
     7621 aaaccgtttg taacgtttgt aatttacctg tgtaccttac tctcggggaa tcaaatgaat
     7681 ctcactttac tgattcaaag tgcaattttt gccattcttc ccgcaattct atttttattt
     7741 gcttccgatt tgggagggct tttcagcggg tttttggcag cagctctgat gatttttcag
     7801 gaatggaatg cgctccacac cactcagatt ctgacgattc attcccgttt ggaaatgagc
     7861 gaatttttag ccgaagtatt atttgcagcg tttgcatact ttattttcaa atggttccga
     7921 cagggaagaa aacaaaccta cttcgctgcg gcagccggag gagtcttggg gctcctgatt
     7981 tatacccgct ttaattttgt ggccatgatt ccggcaatta ttctgttcgg aggcatcgtc
     8041 ttccataaaa aaataaaacg ggggatacgc gaccttctga tctttctgct gtcctttgtg
     8101 atctgtatca gcccttgggt cgttcgatcc tatcatatca ccggaacgat agcgcccgag
     8161 attttggact cttttaaatc cgtggttgtc gaacaaagac tgaaacccat caccgaaacc
     8221 tccgaaattt cggtcgagaa taaagagcct gtccaaccga ctgccgaacc tgttcttcaa
     8281 cagcatatgg aagctacccc tgccatcggc aagcaaattg aaaaagagac ggaaactcaa
     8341 ttaaaatcgt tttcgttgat tcccgatgaa atacgtctga acgttcatcc gttgatcgac
     8401 acaatcggaa atcatttctt ccataacctt ttttcgatcg ctttcatcct tcccgtccaa
     8461 attcaattcg atgatttgaa tcatttatat acagccgaag attctgtttg gagagatcat
     8521 tggaacggga gtctttcggc ggggcagata ctttttctga taatcaatct gatcctgatt
     8581 agtaccggtt tgaatttgct ttggaaaaag gagagctggg caggactttc ggcggcttat
     8641 ttgttgatcg tgtatgcggc cggcttgggg cttgcccgta cttccggcgg tcgttacatc
     8701 gttccgatga actggggcgt tctgttaatc tactcaatcg gtctcgcagt aatcctgaaa
     8761 aaaatcacgc cgatccggac ggatctcgta accggaagta ccgaaatttc aaatcaaaca
     8821 atcggaattg gggatttcgg aaaattagtt ttgattattg gcattttttt catggcttat
     8881 ttttccatga tcttaatcga aaagaatact tctccttcga ttccttccga gaacaagctg
     8941 attgtcacgg aaaaaatgaa agagactttt ccggatcaag attggactca agcggagaca
     9001 cagttacggg agggaaagat gcgccttttt cagggagttg cactttatcc gcgcttttat
     9061 tatttcaatc aaggggaaca tggaacggat aaagcgtacg catatcagct ctattcgcgg
     9121 atcatcttca aaattctttt cgataatgga gaatcggata tcgtccttcc gctggataag
     9181 ttccccgata cttttccgga tcaatcttcc gtcgcagtca ttggctgttt ggagcccggc
     9241 agttcctata tcaaagggat cgctgtgatc gggcagacgc cggacggaaa agaatttcac
     9301 tacttacgct caccgatgaa agaaatagcc tgtccgatcc ccgagccggt ttgtccggaa
     9361 gtaaataaat gttactgaac tttatttagc agccaaaacc tgttcaaaca ccgcctcata
     9421 ttgtttggca atccgtttaa tatcatattt tttggccgat tccaggctgt tttggcgaaa
     9481 gctccggagc atttccggac tttccagcag cgctcgtaac gccgaagcaa aagaatcggt
     9541 atcctcggga tcaaaactga aaccgttttt cccttcttct ataatttccg gatttccgcc
     9601 ggcgttactt aataccaaag ccaatccgca ggtcatagcc tgtattccgg aaacgggtaa
     9661 gccttccgat cgggagggaa gaaacaggat atcgctttgt ttaaatattt ccgtaacgcg
     9721 ctcgggtgct acccatcccg ttatagaaat tttgttctgt aaattcaatc gttcgatcag
     9781 ctcttcgatt ttttcgcgat cctgtccgtc gccgatcaat gtacaatgcc aattcaaatc
     9841 ttttatcgcg gcgcagactt taaccagctg ttccaaattt ttctggggaa cgaatcttcc
     9901 ggcaaacaca atctcgggaa ccgaatgagg ttcaattcgt tcgattcgaa cttgatcata
     9961 gtcaattccg ttcggaatga cctgtatttc caccggatat tttcgctgtg ccattctttt
    10021 cgagaactca ctgaccgcta cgattgcgga agcatgcttc caaaaggtat gtgtaaaagg
    10081 ataaatgaat ttaaaccatt taccggtttt ttcgggagat gcgtcgggaa tatctcccaa
    10141 atgaaccgtt aaaacgtagg gaatatgttt tttccccgat ataaactttg ccggcgggcc
    10201 gccgggtacc gcaaaatgcg catggataat atccggcgaa aaatccttca tttgataaac
    10261 acaataacgg gaagcagcca atataaaaga agccatcggc agcagttttg cccggtatgc
    10321 ttccttacga tgagatttta atcggataat ttcgacgccg tccaggactt cccgatcggg
    10381 taaatctccg aactgagctg tgataacccg aacctcattt ccgtcttgaa ccaatgcaca
    10441 agccagatcg cgggcaatca accccccgcc tccaccgatc ggtggaaatt catgaataac
    10501 ggttaagatt ttcatttatc ggatttgaaa ttaatcaact ttttaaccgt ataagtgcgt
    10561 tttccctgcg attcataata tgatcgggtg agcatttcag ccaataatcc catcataatc
    10621 aattgaaagc ctaaaataaa aatcatgata ctgaaagtga acaggggaga tgccagtacg
    10681 gaaacgcccc agcccaatcg acggacagcc aaaaacagca gcaacaaccc gccgatgatc
    10741 gttaagaata atcccgatcc cccgaacaga taaatcggct tatcggaata agaagataaa
    10801 aacttaaccg taaataagtc cagaattact tttgctgttc gttccaatcc gtatttcggt
    10861 ttcccgaacc ggcgcggatg atgtttgact tcgacttcga taattcttgc cccgaccgta
    10921 ttggcataag ccggaataaa acgatgcatt tctccgtaca atcggaatcc ggtaatcact
    10981 tcgcgtcgat aggctttcaa agtgcaacca tagtcatgaa gaggaactcc ggttacatga
    11041 ccgatgattt tattggcgat gacggaagga attttcctgg ataaattatc ctgtcggctt
    11101 tttctccagc cgctaaccag atcatatccc tcatcgattt ttccgagcat catcggaata
    11161 tcggcaggat cattttgcaa atcggcgtca atcaagataa ttatctctcc ggtcgaataa
    11221 tcaacacctg cggcgatggc agctgtctgt ccaaaatttc tgcgcaaacc gatcactttt
    11281 acatgtgcgg gatctttttc agccaccttt gttaattcct caaaactgcc gtctacggaa
    11341 ccgtcatcaa ccaggatgaa ttcccattct ttatcaaaat cctttaatgc cttcaccatt
    11401 tcttcataca ataaaggaat attgtcatgt tcgttataaa ccggagcaat cacagataag
    11461 tacatatttt ttcctttttg aatcgacttt aatcaatcga tttcagccca ctcctccatg
    11521 acggaattca attcaatttc tatggaatta taccgaattc ccagctctgc gattttttga
    11581 tgatcttctt tttgcgaaac catttgttga gaaatttcgt tgagcatttt ttccaagacc
    11641 gaaatccttt tttccagttc ttcctttttt ttctgccttc taaatatctc attatttgac
    11701 aacttttgca agggaggatt ttctcttatc tcaggagatt tcctttccgt tgaatcaatt
    11761 tgatctttta attcgctgat cccggatttc ttttgatatt ccttatattc cgaataattt
    11821 ccgcgaaata tttccaacgt ttttttatcg gggttcactt cccaaatttg agtcgcgata
    11881 gcatctacta agtatcgatc atgggaaacg agaagaatcg tcccattaaa atcctttaat
    11941 aatgcttgta acgtctcctg agcttcgaga tccaagtgat tcatcggctc atcaagcaac
    12001 agtaaattcg cattttgaag agctaaaatt gccaaagcga gtcgaccgcg ctcaccaccg
    12061 gataacgtct cggttgtttt aaaaacatca tcaccggtaa ataaaaatcg agccagataa
    12121 gatcggatct cggcaggcaa catttgcgga gccgctgcgg atatttcgtc cataagcgtc
    12181 gctttcggat ttaatttttc atgagcctga gcaaaatatc ccacttttaa attagccccc
    12241 atttccactt cacccgctaa cggaggaatc tcatttaaga tggttttgat aaaagtactt
    12301 tttcccacac cgttcggacc gagaatggcg acacactcac cgcgttgcaa caccacatcg
    12361 ggaatggata tcaatttttt atgatcatcc aaataaccga tttcaagatt ttttgttctg
    12421 agaacaatat ctccggatcg gatacttgac gaaagatttg tttgaatttt ccgatgcaag
    12481 atcggattcg ttattttcga ttcttccagc aatctttcca accgtgttcg ccgtccttgt
    12541 gcctgccttg tattttggcc ttcgatgttt cggcgaatat attcttcctc ttttgcaata
    12601 aattcttgtt gactttcaaa ttcagctttt tggcgttcat atcgagcctg cctttgttgc
    12661 agataggcat cataattccc atgatagatt tcaaaagaag aatccaattc ccaaatgact
    12721 tcaactgttt gatttaagaa gtaccggtca tgggaaacaa ttaaggctcc tcctttatat
    12781 tctttcagaa aagattcgag ccactctatg gcattaatat ccaaatggtt ggtcggctca
    12841 tctaaaatca aaagatcggg attcattaat aatatttttg ccaaatatgc ccgtgttttt
    12901 tggcctccgg aaagctgata ccaagggcga ttttcttcac tttttgaaaa accgagccct
    12961 tgtaaaacct gatggattct tgtttcgaaa acgtatccgc cacggctttc gaaagtattt
    13021 tgaagattac cataacttga taatagctcc gaaacattat tcccgtttgc taaatcctgt
    13081 tccatttggt tcagtttgct tttcatcaga attaaatccg aaaaaacgga caaacattcc
    13141 tgatatggtg atttctcgaa ttcagcctca aaattttgtg gcaaataacc gatcgttatt
    13201 ttttttgccc gttggacaga tccggaatca gaaggagtaa tccccattaa tatttgcaat
    13261 aaagttgatt taccggaacc gttcggtccg acaaggccaa ttttcgcacc tctcggaatg
    13321 gaaagggtta tttctgaaaa aatatcttca gcgccgaaag attttgacaa tttttgagcg
    13381 gatataagag gcattcattt ctcctccaaa agtataacag aaatgtttca cgtgaaacat
    13441 ttaatcgtgt tttcccggac tgttttagga ttttaggaga accacacgat taaaataaat
    13501 attcccctat tccgaaatat acatatagct cttttccgag aagcgcttaa tagtaaaccc
    13561 attgttttac cggttttgtc cggaatttgg aatttaattt cttttatatt cgctaatcct
    13621 cgaaaaaatc gtctcttccc gatgaaatga ctaattctat cttttgaata aaaaattaat
    13681 tgaagcgata tgtatcaaag cgggttatca atgaataaaa actttgaaat tgagatatta
    13741 ta
//
LOCUS       DAOMIV010000003        13024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_4762_length_13024_cov_4.584316, whole genome shotgun sequence.
ACCESSION   DAOMIV010000003 DAOMIV010000000
VERSION     DAOMIV010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 13024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13024
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4762_length_13024_cov_4.584316"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1112)
                     /locus_tag="PKV59_00135"
     CDS             complement(144..1112)
                     /locus_tag="PKV59_00135"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45185.1"
                     /translation="MKKVISLLVFVLLLSGVFAVAVSAADDLIVVGYSQVGAESDWRT
                     ANTESFKSTFTEENGYKLIFDDAMQKPENQIKAIRNFIQQEVDYIVLAPIVETGWETV
                     LGEAKAAGIPVILSDRMIKVTDDSLYVAWVGGNFIKEGHNAIEWLDNYLKENGRDNED
                     INIVLLQGTIGSSAQIGRTQGVEELLADRPKYHLLAKQTGEFTQAKGQEVMESFLKSY
                     PDIDVVIAENDDMGYGAMEAIRAAGKVPGKDIIMISYDAGRRAFEAILKGDLNATIEC
                     NPLHGPRVAEIIQKLEKGEPVEKIQYVDEGVFDIKNAAEVLPTRAY"
     gene            complement(1298..2302)
                     /locus_tag="PKV59_00140"
     CDS             complement(1298..2302)
                     /locus_tag="PKV59_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45186.1"
                     /translation="MIKKQILSYTVFLVSLCLAGCFYRPNAQRSSLVTPTESNLIVVG
                     YAQVGAESDWRVANTQSFKSTFTEENGFELIFIDAQQKPENQIKALRNFIQQEVDYIV
                     LAPIVESGWDSVLKEVKEAGIPLILSDRMVNVEDDSLYVSWVGGNFVKEGEDSVEWLH
                     NYLKEIGRDKEIINIVEMQGTLGSTAEIGRTEGIEKKIAEYPNFRLLEKQTGDFVQSM
                     GQEVMEGFLKKYDDIDVLIAQNDNMAFGAIDAIEAAGLVPGKDIIIISIDAVKAALEE
                     IVAGRINASIECNPLHGPRVAEIIRKLEAGEPVEKKIYVNEEIFDIKNAAERLPTREY
                     "
     gene            complement(2299..3684)
                     /locus_tag="PKV59_00145"
     CDS             complement(2299..3684)
                     /locus_tag="PKV59_00145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQP45187.1"
                     /translation="MTIIMLTMTLIIVVMLAVLIRSTNQYNLIVQNLVTASEFNFDFK
                     ETLDYKMYQYVIGGGDDFDTLDPLSDVSNALNVVEKLKLTTVRADNIKRLQTISAFLM
                     NLRTKIDKIHETHSYQENMTSVDYDIRVLTELIQEKVQEYIFYETNELAVIRERTNQE
                     ITRTIVILGIVSIGIVILLWIFSFRISDSIVRPIRMLSENFKMVGRGDFTVRSEKGYD
                     DEIQMLNDTFDSMVGQIEILLNDVKHEQENLRQTELKLLQAQINPHFLYNTFDTIIYL
                     AEDHQDEKVIEMITSLSVFFRTTLSKGEDIISLREEEVHACSYLEIQKVRYRDILEYE
                     IVIPEELLDYSVPKLTLQPLVENALYHGIKNKRGMGKITVKAWQSQSELFLQVKDNGI
                     GMKQENLEKLRKSVLEEHKIGFGLSNVHERIQLYYGSEYGLSIESEYDLGTEVTIKLP
                     LNLIRSEEQKI"
     gene            complement(3747..5381)
                     /locus_tag="PKV59_00150"
     CDS             complement(3747..5381)
                     /locus_tag="PKV59_00150"
                     /inference="COORDINATES: protein motif:HMM:NF037653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQP45188.1"
                     /translation="MLKVFLVEDEIIVREGIRDRINWVENGFEFSGEASDGELAYPMI
                     QQIKPDIVITDIKMPFMDGLALSRLIRNELPWTKIIILSGYDEFQYAKEAISIGVTDY
                     LLKPIGSAKVLETLLKIRDTIEEERNLREMQIRYRKEMQENENYQRRQFFSQLISDST
                     SVPEILNRANELDIDLAAKSYNIILFICSQKDESSWEKYSGKLVQMEEKIENNFSMRK
                     HMILFRRGLEGWALLIKGTDHESAESVTKRCIVELENLFQSDQSMDYFIGVGMPVERL
                     QQIPQAFQKANRAFVYRYIVEDNKIEYYSFDPKPAITSQNGFSLKLLDPSKLSQSVID
                     RFLRSGLKENVSQFVEDYIESIGLDSGNSLPFRKYIVTNILIGVSAFLDEIGIGKHVI
                     IDSFGDIEQITEKVTTVSYTKELIEKIFDVALDFRDNNSKQKYANLLSAAETYIRENY
                     NSEDISLNLVAQVTNVSPSYFSSIFSQEKGITFIEYLTNVRIEKAKELLRSTTLRSSE
                     ISQAVGYKDSHYFSYLFKKETGLTPREYRTGKEENN"
     gene            5566..5772
                     /gene="tatA"
                     /locus_tag="PKV59_00155"
     CDS             5566..5772
                     /gene="tatA"
                     /locus_tag="PKV59_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HQP45189.1"
                     /translation="MWRPGFAEILIVLVIVILLFGPGRIAKVAKEIGASITAFKEGLN
                     EDSDKKKTSEETQSDDENAKSNHP"
     gene            5798..6178
                     /locus_tag="PKV59_00160"
     CDS             5798..6178
                     /locus_tag="PKV59_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45190.1"
                     /translation="MGFFNIGIPELILVLTIMLVFLGPDKLRESSRNFARFIRKVVRS
                     ETWRNFSVMYHEMKHYPEEIMKEVNLEETMKELNEINARTQAELDRIQNSVRSMPKAV
                     DSDSSESNPSDSDKENTPETKDSL"
     gene            6175..6900
                     /gene="tatC"
                     /locus_tag="PKV59_00165"
     CDS             6175..6900
                     /gene="tatC"
                     /locus_tag="PKV59_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HQP45191.1"
                     /translation="MNQEESSTDFKEFLEGMSEHLTELRNRLIVIVLSLCFAIIIAFF
                     FSDSIMAFLARPIGGLEKLQSIEITENFTAVFRVALLAGLVLDSPIIFYEILAFILPA
                     LRSNEKRILFTFLPLLILFFIAGILFAFFILLPAAIPFLTSFTDIPTTIRTNSYFSFT
                     TNMLFWIGIVFEMPIFIYFLTKVKIVNSGLLLKNWRQAVVICAILAMLITPTVDPVNM
                     TILLIPLVLLYFLSILFAKIAER"
     gene            7032..7775
                     /locus_tag="PKV59_00170"
     CDS             7032..7775
                     /locus_tag="PKV59_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HQP45192.1"
                     /translation="MKEINQQNKTKFIRGTLENCDQAGDLAVVIDVLRAFTTAAYAFS
                     KGAKTIYCVAEVEKALELKSCIPGSLTMGEVNGLSIPEFDFNNSPTALMQHDIQEKII
                     IQRTTAGTQGLIRSSHAKTLVAASLVTAKATAEFIRLRSPEEIFFVETGITSDGRGDE
                     DTACADYIEGILTGNPIPEEQIIERVRNSVNGRRFIQNASPDLPAEDLEFVCDINRFH
                     WIIQAERVDNQIVRLSKKEKFQWNFKNPS"
     gene            7751..8806
                     /locus_tag="PKV59_00175"
     CDS             7751..8806
                     /locus_tag="PKV59_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQP45193.1"
                     /translation="MEFQKSVLITKKVRGEDFHSLEPDYLIFEPKETNAEFTREELDE
                     ILPKVTAIVAITDVNAEMIAKALSLKVIVANGAGFDNIDITAATALRIPVINVPDATA
                     YSTVELAMALILDVSRRVSELDRLLRRNADNVSDFFSIGKNPGQNLAGKTLGIIGLGH
                     IGMTLAEMCWPFRLNIQYYNRHRLPLSQEKGIRYVPFEELLETSDIISIHCPLNNSTR
                     NMINYEAFSRMKQGAIFINTARGPIADYDALTYFLENGKLKGAGLDVYPAEPEIPVKL
                     FKLENVVLTPHIGTNTVETRQGMADAICDVIRTVCEAEPGQIFKNVINREIYQREPVT
                     SQPDESSEIHPEKESES"
     gene            8803..10815
                     /locus_tag="PKV59_00180"
     CDS             8803..10815
                     /locus_tag="PKV59_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45194.1"
                     /translation="MSLKKSFFLFLFLISLISQIHPAFAESEPINSDDVTLIGINDAI
                     YSLTEAKTAEITTADQLRATLEVNDVIVEKNRVLVRIIIWGLPVTWGDKVIDQNRIYG
                     SYLPVAELGIPSGKWLTPSSGSRYSLLSNGNTLVIAGLLEFLTDEQPAVVSFNFNQIP
                     FDMEILSEGSTMILELEKGDHQERSERSPLSDQKDGVVLSLMNTAQTTKTTMIQPGFA
                     TIREDESISKIGWIKVERSDGKKYLLTRTDPYGFNLSDDSKTILVDSYTFMPLSSSDP
                     LKIGLDYVYVKRSGNHEVELSFPAPLDFTSEKAALNVEMPLDEFSGKINGYQVFNKTI
                     QGEEMTILRLFMNNDPHISSISFKDSLAEDDFTYSNCGYLPDSDQFACDVQITKLGQS
                     DLTLYFDSFEYRIDGNWEIKWTPVSIAFASNPEPVPGTRLEIDYSKLGSSSSNEIHEL
                     TDLLRSFSDSLTANPGWILQKAQTQAVAAGGEYPDLVQHGDEVLKNNRTVSESWDQID
                     SDGYVINNISLTRDTKGNILTGTWNRSDCQILLPQGYKVPYESGPSDTIYPFLYGNEF
                     FTLLNTSAEFIGRKDCEIDGQADWCYTFEHSLSSSPDSGQPTFMNRYTFLIDPETGEI
                     LREETECQLGGPGTPLQPCIQSKTVEISYRKSLDSELQTMIDSIIF"
     gene            10828..12150
                     /locus_tag="PKV59_00185"
     CDS             10828..12150
                     /locus_tag="PKV59_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HQP45195.1"
                     /translation="MTVSAKFTFPEKFLWGTATSAHQVEGENNNNNWSLWEAQPGRIR
                     NGDVSGKACDWWKGRRWQEDFLRAADTGQNTHRMSIEWSRIQPKENLWDESAIDYYRE
                     MIRGMISRGLRPLITLHHFTDPIWFYEMGGWQNEKAPEYFLRFAEKIIPAFKPLVKDW
                     ITINEPNVYILCGFISGHFPPGNINNFSAANIVLRNMIKAHAAVYHLIHEQQPEASVG
                     IAIHFRDLFPKTNLPPDRWMTRLAHHLFNNSFTDTISSGKFHYGPYFIHIPQAKNTQD
                     FLGLNFYSGNQVRFTFNPNNFLSYPEGASLSDTGFIANEPESFSRALKWAAGYGKPIL
                     ITENGTESDEDTFRRDYLAAHIHRLWRAWSNGIPVKGYYHWSLIDNFEWERGWSQRFG
                     LWRLNVETQVRTKRLSADFYERICRKNSLDSEDMETFAPRIYREIFPV"
     gene            12282..>13024
                     /gene="recF"
                     /locus_tag="PKV59_00190"
     CDS             12282..>13024
                     /gene="recF"
                     /locus_tag="PKV59_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HQP45196.1"
                     /translation="MILQSLSLTNFRSYVRLDVEFPQGITILSGGNAQGKTSLLESLF
                     YCATFAPILAGNDRQLINFEAQKQDLAVARIVVRYSRDQQNHQLEVRIIQESQNRITR
                     TRKEILWNGQKLRAQNAIGKFPAVLFIPQMTAILEGPPQERRRYLNILLSQSEPGYAQ
                     TLTEYKQALVQRNALLRQIADKKSDPAQLEYWDELLSSRGAVLIQKRIKALETLNEYA
                     EEIHLQLTDQQEKLALIYNPSFDPLNPDRS"
BASE COUNT         3743 a         2858 c         2607 g         3816 t
ORIGIN      
        1 caactccggg aaaagattta ctgatatgcc tcattgttag tagttcattg ctcatattta
       61 aattcccaaa gtggttttca aattcgtttg aaaaaaatgg caggtggtct ttcccgcctg
      121 ccattttttc gaacactcaa agactaataa gcccgtgtcg gaagaacttc agctgcattc
      181 ttgatatcga atacgccttc atctacgtat tgaatcttct caaccggttc acccttttcc
      241 agtttctgga taatttcagc gacacgcgga ccgtgaagcg ggttgcattc aatggtcgca
      301 ttcagatcac ccttcaggat ggcttcaaaa gctcgacgac cggcatcata ggaaatcatg
      361 atgatatctt ttccggggac tttaccggca gcccgaatgg cttccatcgc accgtatccc
      421 atatcatcat tctcagcgat gaccacatcg atatccggat aggatttcag gaatgattcc
      481 ataacttcct gacctttggc ctgagtaaat tcacccgtct gtttggcaag cagatgatat
      541 tttggccgat cggcaagtaa ttcttccacg ccctgtgtac gaccgatttg tgcggaagaa
      601 ccgatcgtcc cttgaagtag aacaatgttg atatcttcgt tatctcgacc gttctctttc
      661 agatagttat ccaaccattc gatcgcgtta tgaccttcct tgataaagtt cccgccgacc
      721 catgcgacat aaagggaatc atcggtaact ttgatcatcc ggtcagaaag aatgacgggg
      781 attccggcgg cttttgcttc gcccaacacc gtctcccatc cggtttcaac gatcggagct
      841 aaaacgatgt aatccacttc ctgttgaatg aagtttcgaa ttgctttaat ctggttttca
      901 ggcttctgca tcgcgtcatc aaaaatcagc ttatagccgt tttcttcggt aaatgttgat
      961 ttaaaggact cggtattggc agtccgccag tctgattccg cgcctacttg ggaataaccg
     1021 acaacaatca aatcatcggc agcgcttacg gcgacagcaa aaacgccgga caacaataaa
     1081 acaaaaacaa gtaaactaat aaccttcttc atcttccctc ctaaattgat ttcggcttga
     1141 atagccggct gaatattgtt aaattcaatt gtatggataa tccgaaaaag actctacgga
     1201 aaatcttatg gagaaagttt agttttttaa tcgaattatt tcgaaaagtg ggattttatc
     1261 tttaaaaagt taattttatt taaagtccat caaatattta atattcccga gtaggtaatc
     1321 gttcggcggc atttttaatg tcaaagattt cctcgtttac gtaaatcttt ttctcaaccg
     1381 gttcacccgc ttccaattta cggataatct cagcgacccg cggaccatga agcgggttgc
     1441 attcaatgga agcattaatt cttccggcga cgatttcttc caaagctgct ttcaccgcat
     1501 caatagaaat gataataata tctttcccgg ggactaatcc ggcagcttca atcgcatcaa
     1561 tcgctccgaa agccatatta tcattttgag caatcaaaac atcaatatcg tcatatttct
     1621 ttaaaaagcc ctccatcact tcctgcccca tcgactgaac aaaatcaccc gtttgttttt
     1681 ccaataatct gaaattagga tattcagcta tttttttttc gattccctcc gtcctgccta
     1741 tttccgcagt ggagcctaaa gttccctgca tttcgacgat attaatgatt tctttatctc
     1801 tccctatttc ctttaggtaa ttatgaagcc attcaacgga atcttcaccc tctttcacaa
     1861 aattaccgcc gacccatgaa acatacaggg aatcatcttc aacattgacc attctgtccg
     1921 aaagaattaa gggaattccg gcttccttta cttctttcag aactgaatcc caaccggatt
     1981 caacaatcgg agccaaaacg atataatcaa cttcctgctg aataaagttt cttaatgcct
     2041 taatttgatt ttccggtttt tgttgagcgt cgataaatat caattcaaac ccgttttcct
     2101 ctgtaaaagt ggatttaaaa gattgggtat tggcaactcg ccaatctgat tccgctccga
     2161 cctgtgcata tcccactaca atcaaattac tttcggtcgg tgtgacaagt gaggagcgct
     2221 gtgcattcgg acggtaaaaa catccggcta gacataaact taccaaaaaa acggtataag
     2281 acaaaatctg tttcttaatc atatcttttg ctcttccgat cgtatcagat ttaatggaag
     2341 ttttatagtg acttctgtcc ctaaatcata ttcactttcg atagataatc cgtattcgct
     2401 tccgtaataa agctgaattc tttcatggac attggataag ccgaatccaa ttttgtgttc
     2461 ttctaaaacg gattttcgta atttttccaa attttcctgt ttcataccga taccgttatc
     2521 ctttacctga aggaataatt cactttgaga ttgccaggct ttgacggtaa tttttcccat
     2581 tccccgttta tttttaattc catggtacaa agcgttttcg acaagaggct gtaaagtcag
     2641 cttagggacg ctgtaatcta aaagctcttc cggaattaca atctcatatt ccaaaatatc
     2701 ccgatagcgt actttttgaa tctcgagata actgcaggca tgaacttctt cttcccgaag
     2761 cgaaatgata tcttcgccct tacttaaggt cgttcggaaa aatacggata gggaagtaat
     2821 catttcaatc actttttcat cctgatgatc ttcggcaaga taaatgattg tatcgaaagt
     2881 gttatacaag aaatgcgggt tgatctgtgc ctgtagaagt ttcaattccg tttgccgaag
     2941 attttcctgc tcatgcttga catcattcaa aaggatttcg atttggccaa ccatactgtc
     3001 aaatgtatca ttcaacattt gaatttcatc gtcatatcct ttttcggaac gaacagtaaa
     3061 atctccccga ccgaccatct tgaaattttc agaaagcatt cgaatcggac gaacaatact
     3121 gtcggaaata cggaaagaaa agatccataa caagatgacg ataccaatgg aaacgatgcc
     3181 caaaatgacg atagtccgtg tgatttcctg attggtccgt tcacgaataa ccgctaactc
     3241 gttagtttca taaaaaatgt attcttgaac tttctcttga atcaactccg tcaaaactcg
     3301 gatatcataa tccacacttg tcatattttc ttgataagaa tgtgtttcat ggatcttgtc
     3361 aattttggtc cttaaattca tcaggaaagc actgattgtt tggagccgct tgatattgtc
     3421 cgcccggact gttgttaatt tcaatttttc gaccacattc agcgcattac ttacatcgga
     3481 aagcggatcc aatgtatcaa aatcatcacc tccgccgatt acatattggt acatcttata
     3541 atccagcgtc tctttaaaat cgaaattaaa ctcgctggcg gtgacaaggt tttgcacgat
     3601 caaattatat tggttggtag aacgaattaa tactgccaac ataacaacaa taattaaagt
     3661 cattgttagc atgatgatcg tcatcagcat attcaactta aaacttaaag aggaaaatct
     3721 aattcgattg gaaaagtatt tcctgttcaa ttattctctt ctttcccggt acggtattcc
     3781 ctcggagtta atccggtctc tttcttgaaa agataactga aataatgcga atccttatac
     3841 ccgaccgctt ggctgatttc cgaactgcgt aaagtcgtcg atcggagaag ctctttcgct
     3901 ttctcaatac gaacattggt taaatattcg ataaaagtga tccctttttc ctggctgaaa
     3961 atcgaactga aataagaagg gctgacattt gtcacttgtg caaccagatt taaggagata
     4021 tcttccgaat tgtaattttc acggatataa gtttcggcgg cagataaaag attggcatat
     4081 ttctgtttgc tattattgtc tcgaaaatcc aaagccacat caaatatctt ctcaattaat
     4141 tcctttgtat atgatacggt cgtgaccttc tccgtaattt gttcaatgtc cccaaaggaa
     4201 tcaataatga cgtgttttcc gatgccgatc tcatccagaa aagcggaaac accgatcagg
     4261 atattcgtca cgatatactt gcggaaaggc agagaattac cggaatccaa accgatactt
     4321 tcaatataat cctcaacaaa ttgagaaaca ttctccttca acccgcttcg taaaaatcgg
     4381 tcgataacgg attggcttaa tttcgatgga tccaataact tcaaactgaa tccattttgg
     4441 cttgttattg cgggtttggg atcgaacgaa taatactcga ttttattatc ttctacgata
     4501 tagcgataaa caaaagcccg gttcgctttt tgaaatgctt gcgggatctg ctgtaaacgt
     4561 tccaccggca tcccgactcc gataaaataa tccatgcttt gatcggattg aaaaagattt
     4621 tccaattcga cgatacaccg ttttgttaca gactcggctg attcatgatc tgtgcctttt
     4681 atcagcaaag cccagccttc aagcccgcgg cgaaacaaaa tcatgtgttt ccgcattgag
     4741 aaattatttt cgattttctc ttccatttgg accagtttac cggaatattt ttcccatgag
     4801 gattcatctt tttgagagca gataaacaag atgatgttat aacttttcgc tgctaaatca
     4861 atatccaatt cattcgcacg atttaatatt tccggaaccg aagttgaatc ggaaatgagc
     4921 tgtgaaaaaa attgacgtcg ctgataattt tcattttctt gcatttcttt tcgataacga
     4981 atttgcattt ctcgcagatt tcgttcttct tcgatcgtat cgcgaatttt caatagggtt
     5041 tccaaaactt tggcacttcc gatcggtttc aacagatagt ccgtcacacc gatgctgata
     5101 gcttccttcg catattgaaa ttcatcataa ccgcttagga taataatttt tgtccatggt
     5161 aattcgtttc ggattaatcg gctcaacgcc aacccatcca taaacggcat ttttatatcc
     5221 gttatgacaa tgtccggttt aatttgttgg atcatcggat aagccaactc gccgtcactg
     5281 gcttctccgg agaattcgaa tccgttttcg acccaattaa ttctgtcacg aattccttca
     5341 cgaactatga tttcatcctc aaccaagaat acttttaaca taatgcccta ttcattttga
     5401 aaatttaaaa cgattatagc taattttacc gataatttgc tgtctgactt tctattattc
     5461 ggcctgcaaa cctgaatgat tttcggcggt ataatccctg ttagaaaaat agtcgtttta
     5521 tggatcaggc tgatcgaacc caatgatcca tggagaggaa atcgtatgtg gagacccgga
     5581 ttcgctgaga ttctgattgt ccttgtcatc gtgatcttgc tatttggacc agggcgaata
     5641 gcaaaggtcg ccaaggaaat cggagcaagc attaccgctt tcaaagaagg attgaacgaa
     5701 gattccgata aaaaaaagac ttcggaagaa acgcaatccg atgatgaaaa tgcaaaatca
     5761 aatcatccct aatctcgttc gttattaatt attcagaatg ggctttttta atatcggtat
     5821 tccggaactg attctggtac tgacaattat gttggtcttc ttgggacccg acaaattacg
     5881 ggaaagttcc cgtaatttcg ctcggtttat tcgtaaggtg gttcgttccg aaacatggcg
     5941 caatttttcc gtgatgtatc atgaaatgaa acactatccg gaagaaatta tgaaagaggt
     6001 aaaccttgaa gaaacgatga aggaattaaa tgaaataaat gcccggacac aggcggaatt
     6061 ggaccggatt caaaacagcg ttcgttcaat gccgaaggca gtcgattccg attcatccga
     6121 atccaacccc tcggattcgg ataaggagaa cactccggaa acaaaggatt ccttatgaat
     6181 caagaggaat cctctaccga cttcaaagaa tttttagaag ggatgtcgga acatttaacc
     6241 gagttacgaa atcggctgat tgttattgtt ttatcattat gtttcgcaat tattattgct
     6301 ttcttctttt ctgattcgat tatggcattt cttgcccgac cgatcggcgg actggaaaaa
     6361 ctgcaatcca tcgaaatcac ggagaatttc acagcagtct tccgagtggc attgcttgcc
     6421 ggattggttc tggacagtcc cattattttt tacgaaattt tagccttcat tttacctgct
     6481 ttaaggagca atgaaaaaag gatcttgttt acatttttgc cgcttttaat cctctttttc
     6541 atcgcgggaa ttctttttgc attctttatt cttttgccgg cagcaattcc tttcttaact
     6601 tcttttaccg atatcccgac gacaatccgt acgaacagct atttttcatt tacgacaaat
     6661 atgctattct ggatcggaat tgtctttgaa atgccgattt ttatctactt tttgacaaaa
     6721 gtgaaaattg taaattccgg gttgctatta aaaaactggc ggcaagccgt cgtcatttgt
     6781 gccattcttg ccatgctcat cactccaaca gtggatcccg tcaatatgac gatcctctta
     6841 atccctttgg ttttacttta ttttctcagt attttgtttg caaaaattgc ggaacgctga
     6901 ttcgatcttc ctcagggaac actctcggtt taaaaaaatc cggttacata aattcgaaaa
     6961 cttcccgaaa ttttgcaatg ggtttaaaag aaagggatct tttattataa ttattttcgg
     7021 gtgaaatata tttgaaagaa attaatcagc aaaacaagac aaaatttatc cgcggaacct
     7081 tggaaaactg tgatcaagcc ggagatttag cagtggtgat cgatgtactg cgcgctttca
     7141 caaccgcagc atatgctttt tcaaagggag caaaaacaat ttattgtgtc gccgaagttg
     7201 aaaaagcact tgaattaaaa tcatgcattc ccggcagtct gacgatggga gaagtaaacg
     7261 gtctcagcat tccggagttc gatttcaata attcaccgac tgctttgatg cagcatgaca
     7321 ttcaagaaaa aataatcatt caaaggacga ctgccggaac acagggactg atacgttctt
     7381 ctcatgccaa aacgctggtc gccgcaagcc tcgtaacggc aaaagcaacc gcagaattta
     7441 tccgtttgcg gtcaccggaa gagatctttt ttgttgagac gggaattact tctgacggaa
     7501 gaggagacga agacactgcc tgcgcagact atatcgaagg gattcttacc ggtaacccta
     7561 ttccggaaga acagataatc gaacgggttc gaaactctgt caacggccga cgttttattc
     7621 aaaatgcctc acccgattta ccggccgagg atttggaatt tgtttgtgat ataaatcgtt
     7681 ttcattggat catacaagct gaaagggtgg acaatcaaat cgtccgatta tcaaaaaagg
     7741 agaaatttca atggaatttc aaaaatccgt cttgatcaca aaaaaagtcc ggggggaaga
     7801 ttttcattct ctggaaccgg attatctgat ctttgaaccg aaggaaacga atgccgaatt
     7861 cacgcgcgaa gaattggatg aaattcttcc gaaagtgact gcgattgtgg cgatcaccga
     7921 tgtcaacgcc gaaatgatcg ccaaagctct ttcactgaaa gtcatcgtcg ccaacggagc
     7981 cggttttgat aatatcgata taaccgcagc caccgctctt cgaatccctg tgatcaatgt
     8041 cccggatgcc accgcttatt ccaccgtcga gctggcaatg gctctaatcc tggatgtcag
     8101 tcgtcgggtt tccgaattgg accggttgct gagaagaaac gcggataatg tctcggactt
     8161 tttttcgatc ggcaagaatc cggggcaaaa tttagccggg aaaacattgg gaattatcgg
     8221 cttgggtcat atcgggatga ctttagctga aatgtgttgg ccatttcgat taaatattca
     8281 atattacaac cgccaccggt tgccgttatc tcaagaaaaa ggcattcgat atgttccttt
     8341 tgaagaattg ctggaaactt ccgacatcat ttcgatccat tgtccgttga ataactccac
     8401 ccgcaacatg attaattacg aggcattttc aaggatgaaa cagggcgcca tcttcatcaa
     8461 taccgctcgc ggaccgattg cggactatga cgcgttaacc tattttctcg aaaacgggaa
     8521 attgaaaggt gccggattgg atgtttatcc ggcggaaccc gaaattcccg tcaagctatt
     8581 caaattggaa aacgtggtcc ttactccgca tatcggtacc aataccgtcg aaacaagaca
     8641 gggaatggcg gatgccatct gcgacgttat ccgtacggtc tgcgaagcgg aaccgggaca
     8701 aattttcaaa aatgttatta atcgagaaat ctaccaaaga gaaccggtta cgtcgcaacc
     8761 cgatgaaagt tctgagattc atcccgaaaa ggaatctgaa agttgagttt aaaaaagtct
     8821 ttctttttat ttttattttt gatcagcctt atcagccaaa ttcatcccgc gttcgccgaa
     8881 tccgaaccga ttaattccga tgacgtcaca ttaatcggga tcaatgatgc aatatattcg
     8941 ctgacggaag caaaaaccgc ggaaattact accgccgatc aactccgtgc cacactcgaa
     9001 gtcaatgatg tcatcgttga aaagaaccgg gttttggtgc gaatcataat ttgggggctt
     9061 ccggtcacat ggggtgataa agtcatcgat caaaaccgaa tttacggcag ttatttaccc
     9121 gtcgccgaat tgggaattcc gtcgggaaaa tggctcactc cctcttcggg ctctcgttac
     9181 agtctgttat caaacggaaa tacgctggtt atcgccggat tattggaatt tttaaccgat
     9241 gaacaacctg ccgttgtttc cttcaatttc aatcagattc ccttcgatat ggaaattctt
     9301 tccgaaggat cgacgatgat attggaattg gaaaaaggtg atcaccaaga aagaagcgaa
     9361 cgttcgcccc tgagcgacca aaaagacggg gttgttttat ctttgatgaa taccgctcag
     9421 accaccaaaa ccactatgat tcaaccgggg ttcgcgacaa ttcgtgaaga tgaatccatt
     9481 tcaaagatcg gttggataaa ggtcgaacga tcagacggaa aaaaatattt attaaccagg
     9541 accgatccgt atggatttaa tctcagtgat gattcaaaaa caattctggt ggattcttat
     9601 acctttatgc ctctttcaag ttccgacccg ctgaagatcg gcttggatta tgtttatgtc
     9661 aaacgctcgg gaaatcatga agtcgaatta agctttccgg ctcctctcga tttcacctca
     9721 gagaaagcgg cattgaatgt tgaaatgcca ttggatgaat tctccggcaa aatcaatgga
     9781 tatcaagttt ttaacaaaac catccaaggc gaagaaatga ccatcctcag acttttcatg
     9841 aacaatgatc cgcatatatc ctctatttct tttaaagaca gtctcgccga agatgatttt
     9901 acctattcaa attgcggcta tctgcccgac agcgaccaat ttgcctgtga cgttcaaatc
     9961 acgaaattgg gacaaagtga tttgacattg tatttcgatt cgttcgaata tcggatagac
    10021 ggaaactggg agattaaatg gacaccggta tccatcgctt ttgcgtccaa ccccgagcct
    10081 gtcccgggga cgcggctgga aatcgattat tccaaattgg gctcttcttc ttcaaatgaa
    10141 attcacgaat taaccgattt gctccggtct ttctctgatt ctctcaccgc aaatcccggc
    10201 tggatcctgc aaaaggctca gacacaggca gtcgcagccg gcggagaata tccggatttg
    10261 gttcagcatg gtgatgaggt tctgaaaaat aaccgaaccg tttcggaatc ctgggatcaa
    10321 attgattcag acggatatgt gatcaataat atttcattaa ccagagatac aaaaggcaat
    10381 attctcaccg gaacttggaa tcgatcggat tgtcagattc tccttccgca agggtacaaa
    10441 gtcccttatg aatcggggcc ttccgatacg atttatcctt ttttatacgg taatgaattc
    10501 tttacgctgc tgaatacatc ggctgaattt atcggtcgga aagattgtga aatagacgga
    10561 caagccgatt ggtgttatac ctttgagcat tctttaagct cctcacctga ttccggtcaa
    10621 cctactttca tgaatcgcta tactttcctg attgatcccg aaacaggaga aatcctgcga
    10681 gaagaaacgg aatgccagct tggaggaccg ggaactcctt tgcaaccttg catccaatca
    10741 aaaacggtcg aaatcagtta ccggaaatct ttagattcag aacttcaaac aatgatcgat
    10801 tcaataattt tttaaaagga gagattcgtg acagtttcgg caaagtttac tttccccgaa
    10861 aaatttttat ggggaacagc tacctctgcg catcaagttg aaggtgaaaa caataacaat
    10921 aattggagtc tttgggaagc ccagcccggc cgtatacgaa acggagatgt gagcggcaaa
    10981 gcttgtgact ggtggaaagg gaggcgttgg caggaagact ttcttcgggc agccgatacg
    11041 ggacaaaata cccatcggat gtccatcgaa tggtctcgaa ttcagcctaa agagaatttg
    11101 tgggatgaat cggcaatcga ttattatcgt gaaatgattc gcggcatgat cagccgcgga
    11161 ttacgtcctt tgataacatt gcatcatttc accgatccga tttggtttta tgagatgggc
    11221 ggatggcaaa atgagaaagc accggaatat ttcctgcgtt ttgccgaaaa aatcattccg
    11281 gcatttaaac cgcttgtcaa ggattggata acgatcaacg aaccgaacgt ttatatatta
    11341 tgcggtttta tctccggaca ttttccgccc ggaaatatta acaatttttc ggcggcgaat
    11401 attgtccttc gcaatatgat caaagcccat gcggcggtat atcacctgat tcatgagcaa
    11461 caacccgaag cgtccgtcgg catcgcaatt catttccgtg atttgttccc caaaacaaat
    11521 ttaccgccgg atcgttggat gacaaggctc gcacatcatc tcttcaataa ctcatttacc
    11581 gatacgatat caagcggaaa atttcattac ggaccgtact ttattcatat accgcaggca
    11641 aagaacactc aggatttttt gggcctgaat ttttactccg ggaatcaagt ccgattcact
    11701 tttaacccga acaacttttt atcctacccg gagggagcat ccttgagtga cacggggttc
    11761 atcgcaaatg agccggaatc tttttcaagg gcactgaagt gggcagccgg ctatggcaag
    11821 ccgatcctca tcaccgaaaa cggaaccgaa agtgatgagg atacgttcag acgggattat
    11881 ttagctgctc atattcatcg gctttggcgc gcatggtcaa acggaatacc ggtaaaagga
    11941 tattatcatt ggtcactcat agataatttc gagtgggaac ggggctggag tcaacgattc
    12001 ggactttggc gtttgaacgt tgaaacacaa gttcgcacca aacgtctttc agctgatttt
    12061 tatgaacgga tttgccgtaa gaattctttg gattccgaag atatggaaac ttttgccccg
    12121 cgaatttatc gggagatctt tcccgtttag agtccagacc gaaccggttc tttttgacag
    12181 aaggcaagaa atcgttcgtt atctgattct cagatcaata tttctttgat aaatcatgct
    12241 tcggaagcgg agcatgataa acttacctga agagttcttc tatgattttg caaagccttt
    12301 ctttaacgaa tttccgctcc tatgttcgat tggatgtcga atttcctcaa ggcattacga
    12361 ttctttccgg cggaaatgca caggggaaaa caagtttgct ggaaagcctt ttttactgtg
    12421 ccacttttgc tccgatatta gccggaaacg atcgccaact gattaatttt gaagcgcaaa
    12481 agcaggatct ggcggtcgca cgcatcgttg tccgatactc gcgcgatcag caaaatcatc
    12541 agctcgaagt gagaatcatt caggaaagcc aaaatcggat cactcggacg cgcaaagaga
    12601 ttctctggaa cggacaaaag ctgcgtgccc aaaatgcgat cggtaaattc cccgccgtat
    12661 tatttatccc tcagatgact gccattttgg aggggcctcc gcaagaaaga cgccgatatt
    12721 taaatatctt gctttcacaa tccgaaccgg gttacgccca gactttaacc gagtacaaac
    12781 aggctttggt tcagcgcaac gctttattac gccaaatagc cgacaaaaag tccgatccgg
    12841 cgcagttgga atattgggat gaattgcttt cgagtcgcgg agcggttttg attcaaaaaa
    12901 gaataaaagc gctcgagact ttgaatgagt atgcggaaga aattcatttg cagttgaccg
    12961 atcagcagga aaaattagcc ctgatctata acccatcctt tgatccctta aaccccgacc
    13021 gttc
//
LOCUS       DAOMIV010000004        12811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_4867_length_12811_cov_4.668862, whole genome shotgun sequence.
ACCESSION   DAOMIV010000004 DAOMIV010000000
VERSION     DAOMIV010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4867_length_12811_cov_4.668862"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKV59_00195"
     CDS             <1..535
                     /locus_tag="PKV59_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQP45197.1"
                     /translation="IFICRRDMADNHKNRIFNLSTGLISLCVAAFIFLGIILLIVNIG
                     PSPAEEKEDALAQLTVIPMPTITPTSAIPSEKPDLSVKYISPEGFSVGAYVKIENTQG
                     AGLKIRPDPGTGGEVSFIASEGDIFIIIDGPDERNGYTWWKLQGFDDPSLVGWGASKF
                     FSLVAPAADQIQSSDIQ"
     gene            535..1317
                     /locus_tag="PKV59_00200"
     CDS             535..1317
                     /locus_tag="PKV59_00200"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HQP45198.1"
                     /translation="MSHNQSIHALESANAHSIGKIRRYNEDALVSFQLETMRKDQTDS
                     LGLYVVADGMGGHINGDEASASAVSAFTEQLGNDIIPLFLKDESDSSIIPELLKKAVL
                     FANQSILDKHPGSGTTFTAALIWNEKIYIAHVGDSRLFLIHQNYEMEQLTHDHSLVQM
                     MVDLGEISEEEALTHPRRSVLLRSLGFDKDVEVDLAENKLEAGESLLLCCDGLWSVIK
                     NDQMIKIIREHNDIQEAADALVDAANEAGGPDNISCILVRRK"
     gene            1393..2181
                     /locus_tag="PKV59_00205"
     CDS             1393..2181
                     /locus_tag="PKV59_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQP45199.1"
                     /translation="MAEGNKRYLILVVDDDERIVRMIRLNLEHDGYSVIEANSGMEAI
                     NQVREKLPHLVILDVMMPGMDGFETLSLIRETSQVPVIMLTAKGEEEDRIRGLELGAD
                     DYITKPFSPRELMSRIKAVLRRTEGTSGMDTGEVIEVDEHLKLDFGKREVWLDGQLVK
                     LRPTEYRLLYHLVQNAGWVMTYDQILAKVWGYEYRDETHYVRLYINYLRQKLEKDPSN
                     PKYILTERGIGYRFVDYKKGKLKEGEGETKENPNNPEESHPSDN"
     gene            2323..2817
                     /locus_tag="PKV59_00210"
     CDS             2323..2817
                     /locus_tag="PKV59_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQP45200.1"
                     /translation="MYGKLRVQPVRDLFTMQDAMDRLFNEFFGDSDNLQTEVLAPRVD
                     LKQKENEIDVKLSIPGVDPDKIQISVADNVLTVKAEMEEEKEEKDEKAVYEIREHRWT
                     SYYREIALPCEVEADKAEAEYENGVLKIKLPKAEIVKPKLISISAGKKGEKSTEKIES
                     KTGK"
     gene            2924..3490
                     /locus_tag="PKV59_00215"
     CDS             2924..3490
                     /locus_tag="PKV59_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45201.1"
                     /translation="MQLTDTSLVLIAIMPKLRDMEIARVLGWYRIPMKTAPKILLTDY
                     IAFYQTKDFGEEHKSRIEKYAEVRGVELTTRQELFRDEPDHPRAKEEYYKIQIGPLQA
                     LENPILADRWKRFLFFYTSGEKMFTASTLHDLAITGSEREILWKSLRERRENDPEFFR
                     STEKKEEIPEQLMFLLGNLTLSNLDFNE"
     gene            3541..4956
                     /gene="hydA"
                     /locus_tag="PKV59_00220"
     CDS             3541..4956
                     /gene="hydA"
                     /locus_tag="PKV59_00220"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HQP45202.1"
                     /translation="MEKIIKNGKLVTDSEIFDADILIRDEKIVAVGKEFNESNAEIID
                     ASGKLVLPGGVDAHVHLDLPMPGTVSSDDHYTGTKAAAFGGTTTVIDFANHDLPSLID
                     SFNVWQEKALPNVAIDYGIHQNFTRFNENTLKEIPLLPKMGVTSIKMFTAYNGRMRLQ
                     DGEIFQAMRVAKDEGIISLVHAENGDLIDLLVAEALAAGHTAPVWQARTRPAWGAVES
                     GFRVAALSFSAGKAPLYIVHMNVKEEADMLLYARENGLPIFGETCPQYLLFSEKNLEQ
                     PDGAKWVCSPPMRSEKDNEGLWEALADGIIDVVSTDHCPFLFDGTKPILYEGKPYQQP
                     GKELGKDDFSKIPCGLPGVGDRMPVLWTKGVNEGRISPTRFVQLTCTNPAKIFGIYPQ
                     KGALLPGSDADLAIWDPNKIVSYGVKIAKHRTDYNLYEGTELKGFPEKVFLRGSLIVD
                     GDNWLGKRGGGHFLKRSRFLG"
     gene            4964..6541
                     /locus_tag="PKV59_00225"
     CDS             4964..6541
                     /locus_tag="PKV59_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45203.1"
                     /translation="MRFLTVPIGILSFAAILIFLITRLRKKIGVAWFIGALASFSVWC
                     WIFYLKRTPESVFSIRWQNLELSFILDNISWSYMAATATMLFFMMASAPSRLNPDSSS
                     NDWGFFLLITAIVMATVASDSGWSMVFGWFLFDLLTVGIQFFSHQKIYPFESLEKILF
                     IRIFSIFLAAVAIAIGNSIELNPAQSFLEGQRETILLIACAMRAGLFPFFESFQNENE
                     TRKGLNTFVELSSVALVLPAFIRIDSSAMGNDAYGFFMFFSTLSVICGGLGMIFSNKN
                     ASERRYFILTLTGLSFISALRFQPTASVVWGISLSVLTAPLFLYDIRSRLLNVVQIIS
                     GLLLSGLPRTPAASGWRGLVLVPPSFFDFFLIISFACLLTKFFSDLTVQNPAHPALQD
                     RRMNAIYPFGFMVSVIAYGVIAALSWNFYSDNGMIPASIITLIIAFIIGFRSVWLRFF
                     TQMNTFFSWFREIFFTIWKIFLWIIQFRWLEAPIRWIITGLSAIIDFISGIFEAPGGI
                     LWKFLILIIVLFLVIGI"
     gene            6550..7152
                     /locus_tag="PKV59_00230"
     CDS             6550..7152
                     /locus_tag="PKV59_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45204.1"
                     /translation="MRGLFFTSIIGGALIILGALIIFIRLNQPDSRISLIAYILESVG
                     IVLLLVRIFSPQILISVFLTGTMGAAILGTDQVALHIPLKPDKVNPQGIFRLLLGFIL
                     AVLILSIEPEIAVWIPVPDSILFCSLFLILMGLTTFSLSSRLMYRFLGILNIFSGFQL
                     VYMLLEESVLVFGFMIAIDLLIALLGAYFMSGSQNDEYPT"
     gene            7174..8571
                     /locus_tag="PKV59_00235"
     CDS             7174..8571
                     /locus_tag="PKV59_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQP45205.1"
                     /translation="MNAPLLWFIIPFFFGAFLLLFSESKKLSIILASIVCLFLIAISA
                     TVPINSMVIIGKNSIIFTDSMEILGRTLSLERAAQPLLAFFYSYAFIWILFSAAVRVH
                     RFFVPLVLMETALVIGVTAVSPFIYGAFFVQLAVLIALPIFWNEKMKSGEGIIRFLLY
                     QTLGLIFLAIGGWLASAVELNPSDQYILKRTMVVLLVGFIFWLAIFPFHSWIAILMDE
                     SCPYLSGFVINILQFSSFLILLYFLKSYLWLRTYDPLFIGLRMIGTIMLIIGSLWAVF
                     QKSVQRLQAFLIVAENGIALFLLGLHTTQSFSVFISLLFIRMIASFFWALAVKSLPEN
                     FDFGLNSLQGLYRSDPFISLTLIVSYFSTVGLPFLPGFPYQATFLQLAFSESSSMGTV
                     LSIGYWLLIAAGFRLAVTLLHPAEKAAGKAETANQRALLIIGIAALFLITFFPEPFNR
                     FIAQIQSQFTLISGG"
     gene            8624..9832
                     /locus_tag="PKV59_00240"
     CDS             8624..9832
                     /locus_tag="PKV59_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45206.1"
                     /translation="MAKTWNGGPLNEGFVDLDQLVKRLEWLDNERRNDKNRILAIEER
                     LTDVVSENVLLKNQIKDLEEEIQRYANIQAKIEQNESLIAQLRIDAFRAIEEKDKAHS
                     EREKEIDAARRNGIDSINRTLADLRKSIEPLQDIRKTLLSRNEDSIRLGRMIEELQAK
                     IDAVAMDKDEYARFQKSVEENRRQDAKRVTDIQAEVTAYRKRIEDMRSKFDLVSEGLR
                     KVEQRMSELLAAENERKQSVTDFMEKQSLANLEYEKTWQQWKTAFDDIVAKAAGLDTQ
                     IETLENLQRSVKRSQDTLDEVTQRFDRRTNELVEMQRLMEEKLRQEWANFKADNQKRW
                     TNYSLGQEEIQAEYRRNIEALQARMQAIEDASASALDDVNLRDELYISQLQDLFTMVR
                     GWLEKMGKAS"
     gene            9871..11181
                     /locus_tag="PKV59_00245"
     CDS             9871..11181
                     /locus_tag="PKV59_00245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQP45207.1"
                     /translation="MSQITVIGTGYVGLVTGACFSDLGNNVICLDIDEKRISDLNHGI
                     MPIFEPGLEEIVERSHKAGRLQFTVNYPEALRDAEFVFIAVGTPSGSDGEADMSYVEQ
                     AVIQIADNMDHPIIIVDKSTVPVGTGDWVADIIQKYRDGHMPKFQVVSNPEFLREGSA
                     VADFMHPERIVLGSNDTEACDRVAELYAPLRAPMLVTDIRTAEMIKYASNAFLATKIS
                     FINEIANICESVGANVIDVARGMGLDNRIGSKFLNAGIGWGGSCFPKDVKALAHIAVE
                     YGTQPQLLQAVIDINQTQRRRVVEKLNRMLNGLEGKEIGILGLAFKPDTDDMRESPAA
                     DIIRYLTNEKAIIRAYDPEAMEQAKKILPKEVILCDNPYQVVENADALLLATEWNEFK
                     QLDFKKIHDLMREKNILDGRNLWDPERLSELGFTYVGVGLGKYI"
     gene            complement(11295..12584)
                     /gene="tyrS"
                     /locus_tag="PKV59_00250"
     CDS             complement(11295..12584)
                     /gene="tyrS"
                     /locus_tag="PKV59_00250"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQP45208.1"
                     /translation="MSEQNSIYGELKWREMIYDSMDGLEELLEKEKVTLYNGFDPTAD
                     SLHVGHLVPMLALARFQRYGHHVIALAGEGTGMIGDPSGRSDERNLLSAEEAHYNVEK
                     IKRQLAKFLDFEIKSNPARIMNNGDWLLDLKLIDFLRDTGKYFTVNSMMAKDSVKSRL
                     SRESGLSFTEFSYSLLQAYDFYHLFVTENCVLQTGGSDQWGNITAGCELIRRKTGKNA
                     YGLTYPLVKKKDGTKFGKTAGGAVWLDPERTSPYKFYQFWLNTDDADVIPFLKYFTFF
                     DRQKIDELEFATRENPEDRTAQKALAVEMTKMMHGETALDKAMQASEALFGGEINGLS
                     AADIKEIFADVPSGVIAADSMNGEGLPIVNLLSDCGFMKSKGEVKRAIAEGGVYINNH
                     RVTDVNTSIRKDQFIEGKYLVLRRGKKNYFLLKTEQE"
     gene            complement(12625..>12811)
                     /locus_tag="PKV59_00255"
     CDS             complement(12625..>12811)
                     /locus_tag="PKV59_00255"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQP45209.1"
                     /translation="DEQMRGRGVGEMLVRAAVDRARAEGAKTVDLTSRPSREAANRLY
                     RRCGFEPRETNIYRFTI"
BASE COUNT         3409 a         2766 c         3002 g         3634 t
ORIGIN      
        1 aatcttcata tgccggagag atatggcaga caatcataaa aacagaatat tcaatttatc
       61 aaccggtctg atatcactct gtgtcgcagc ttttatcttt ttgggaatca tcctattaat
      121 cgttaacatc ggcccgagcc ccgccgaaga gaaagaagat gcattagctc aattaacggt
      181 tattccgatg cctaccatca ctccgacttc cgcaattcct tcggaaaagc ccgatctcag
      241 cgtcaaatat atatcaccgg aaggtttttc cgtcggtgca tatgtaaaga ttgagaacac
      301 tcaaggagcc ggattaaaaa tccgacctga cccgggaacc ggcggagaag tctctttcat
      361 agcatcggaa ggggatattt tcattattat cgacggcccc gatgaacgga acggctatac
      421 ttggtggaag ctgcaaggat ttgacgatcc gtccctggtc ggttgggggg catcaaaatt
      481 tttttcattg gttgctccgg ccgcggatca aattcaatca tcggatattc aataatgagt
      541 cataatcaat caattcatgc attggaatcg gcaaatgctc actctatcgg caaaataagg
      601 cgatataacg aagatgcctt ggtttctttc cagcttgaaa ctatgcgtaa agatcaaacc
      661 gattcactcg gcctgtatgt agttgcggac ggaatgggtg gacatatcaa cggtgatgaa
      721 gcatcggcgt ctgccgtatc cgcatttacg gaacaactcg gaaacgatat catcccgttg
      781 ttcctgaaag atgaatcaga ttcctcgatt atcccggaat tgttaaaaaa ggctgttctt
      841 ttcgctaatc aatcgattct ggataagcat cccggcagcg gaacgacttt tacggcggca
      901 ttgatttgga atgaaaaaat ttatattgcc catgtcgggg attcaaggtt atttttgatt
      961 catcaaaatt atgaaatgga gcaattgacc cacgatcatt cgttggtcca aatgatggtt
     1021 gatctgggag aaatcagtga agaagaagct ctgactcatc cgcgtcgcag cgtcttactt
     1081 aggagtctgg ggttcgacaa ggacgtggaa gttgatctcg ccgaaaacaa gctggaagcc
     1141 ggagaatcgt tgctcttgtg ttgcgacggt ctctggtctg tcatcaagaa tgatcaaatg
     1201 atcaaaatca ttcgcgaaca taacgacata caggaagcgg ccgatgcgct ggtagatgcg
     1261 gcgaatgaag cgggaggacc ggataatatc agttgtattc tcgtccgccg aaaataagat
     1321 aaattcataa tacaattaag taacggtttc gggctgttcc gaccagatat tttacgaaag
     1381 atagaggcaa aaatggctga agggaataaa cgctatctga ttttggttgt cgatgatgat
     1441 gagagaattg tcagaatgat tcgtctgaac cttgaacatg acggatactc tgtaatcgaa
     1501 gcaaactccg gaatggaagc gatcaatcag gtgcgcgaga aattgccgca tctcgtgatt
     1561 ctggatgtaa tgatgcccgg gatggacggt tttgagaccc tttctttgat tcgggagacc
     1621 agtcaggttc cggttattat gttaacggca aaaggagagg aagaagatcg gatccgcggg
     1681 ttggaattgg gggcggatga ttatattacc aaacctttca gcccgagaga actcatgagc
     1741 cgcatcaaag ccgttttgcg acgtaccgaa gggacaagcg gtatggatac cggagaagtc
     1801 attgaagtgg atgaacactt gaagctcgat ttcgggaaac gggaagtatg gctggatggc
     1861 caattggtaa aactaaggcc taccgagtat cgtcttcttt atcatttggt gcaaaatgcc
     1921 ggctgggtga tgacgtatga ccagattttg gcgaaagttt ggggatatga atatcgggat
     1981 gaaacccatt atgtccgatt atatattaat tacctgcgac agaaattgga gaaagatccc
     2041 tccaacccga aatatatcct gaccgaacgg ggaatcggat accgattcgt cgattataaa
     2101 aaaggaaaat tgaaagaagg cgaaggggag acgaaagaaa atccgaacaa tccagaggag
     2161 agtcacccct ccgataattg attaatcttt tttttataga attctgataa agcgcatgca
     2221 attttcttat acatgcgctt tattctttaa ggcgaagata gaaaaaagac agcgttctga
     2281 aaagaacggg aaaaacgaaa atggaaataa aaggagggag ctatgtacgg caaattaaga
     2341 gttcaaccag taagagattt atttacgatg caagatgcga tggatcgctt gttcaatgag
     2401 tttttcggag acagcgataa cctgcaaacc gaagttcttg caccaagagt agatttgaaa
     2461 caaaaggaaa atgagattga tgtaaagctc agtatccccg gcgttgaccc ggacaagatt
     2521 cagatttcag tcgctgataa tgttttgaca gtcaaagccg agatggaaga agaaaaagaa
     2581 gagaaagacg agaaagctgt ttacgaaatc agagagcatc ggtggacttc ctattatcgg
     2641 gagattgctc ttccgtgcga agtggaagcc gataaagcgg aagccgaata tgaaaatggc
     2701 gttctgaaga ttaaactgcc gaaggctgag attgtcaaac ccaagctgat cagtatttca
     2761 gccggtaaaa aaggcgaaaa gagtactgaa aagattgaaa gcaaaacagg caaataaagg
     2821 atagacttgt agtatcgaga agccccatct gaaatcagac ggggcttctc cctcttaaat
     2881 ttccgcatgg tcaggaacgt taattatcgt tataatccat tttatgcaac tgacggatac
     2941 ttcgctggtt ctgattgcaa tcatgccaaa attacgcgat atggaaattg cccgtgtttt
     3001 gggttggtat cgaattccga tgaaaactgc gccgaaaata ttgttgaccg attacattgc
     3061 tttttatcag acaaaggatt ttggcgaaga gcataaaagc cgtatcgaaa aatatgcgga
     3121 agttcgcggc gtagaactga cgacacgcca agaactgttc cgggatgaac ccgatcatcc
     3181 gcgagcgaag gaagaatatt ataagattca aatcggcccc ttgcaggcgt tggaaaatcc
     3241 cattctggcc gatcgatgga agcgattttt atttttttac acttccggcg aaaagatgtt
     3301 tacggcaagc acacttcacg atttggcaat taccggttcg gaacgggaaa tcttatggaa
     3361 atctcttcgg gaacggcgag aaaacgatcc ggaattcttt agatcgactg aaaagaaaga
     3421 agaaattcca gaacagttga tgtttctctt ggggaattta accctttcta acttagattt
     3481 taatgagtaa tccgattata gaatttaccg attttgtttt gtggaaatat gggaggataa
     3541 atggaaaaaa ttattaagaa cgggaagttg gtgaccgatt cggagatttt cgacgccgat
     3601 attctgattc gggatgaaaa aatcgtcgct gtcggaaaag agttcaatga atcgaatgcc
     3661 gaaattattg acgcaagcgg gaaactggtt ttgccgggcg gtgttgatgc ccatgtgcat
     3721 ttggatttac ccatgccggg aacggtttct tcggatgatc attataccgg tacaaaagcc
     3781 gctgctttcg gcggaacaac aaccgttatc gacttcgcca accatgatct tccttctctg
     3841 atagacagtt ttaatgtttg gcaggaaaaa gcattgccga atgttgcgat cgattatggg
     3901 attcatcaga atttcacccg atttaatgag aacacgctca aagaaattcc cctccttccg
     3961 aagatgggcg tgacatcgat caaaatgttc acggcatata acgggcgaat gcgtttacag
     4021 gacggagaga tatttcaagc catgcgtgtt gcgaaagacg aaggcatcat ctcactggtg
     4081 catgccgaaa acggcgatct gatcgacctt ttggttgccg aagcgcttgc cgcgggacat
     4141 acagctccgg tttggcaagc ccgcacccgt ccggcatggg gagccgttga atccggcttt
     4201 cgagtggcgg cattatcgtt cagtgccgga aaggctccgt tgtatattgt ccatatgaac
     4261 gtaaaagaag aagcggatat gttgctttac gcacgggaaa acgggctccc gattttcgga
     4321 gaaacttgtc cccaatatct gttattctct gaaaaaaatc tggaacagcc tgacggcgcc
     4381 aaatgggttt gttcgccgcc gatgcgatct gaaaaagaca acgagggact ttgggaggca
     4441 ttggcagacg gaataatcga tgtggtttcc acagatcatt gtcctttctt atttgacggg
     4501 acaaaaccga tactctatga aggaaaacca tatcaacaac ccggaaaaga gctcggcaaa
     4561 gacgactttt caaaaattcc ctgcggcttg cccggcgtcg gcgatcgcat gccggtactt
     4621 tggacaaaag gtgttaacga agggaggatc agcccgactc gttttgttca attaacctgt
     4681 acgaatccgg caaaaatttt cggaatttac ccgcaaaaag gggcactctt gcccggttcg
     4741 gatgcggatc tggcgatttg ggatccgaat aaaatcgttt cttacggagt caagattgca
     4801 aaacatcgaa ccgattacaa tctttatgaa ggaacggaac tgaaagggtt cccggaaaaa
     4861 gtctttttaa gagggagttt aattgtcgat ggggacaatt ggctcggaaa acgcggcgga
     4921 ggacattttc tgaaacggtc ccgttttttg ggatgattcg aatatgcgtt ttctgaccgt
     4981 tccgatcggc attctttctt ttgcggcaat tctaattttt ctgatcacga ggctgcgaaa
     5041 aaagatcggc gtcgcatggt tcatcggtgc attggcttct ttttcggtct ggtgttggat
     5101 tttttattta aaacgaacac cggaaagcgt tttttcgatt cgttggcaaa atttagaact
     5161 cagctttatt ctggataata tttcatggtc ttacatggcg gcaacagcca caatgctttt
     5221 ttttatgatg gcttccgctc cttcccgctt aaatccggac agttcgtcaa atgattgggg
     5281 attttttctc ctgattaccg caatcgttat ggctacagtc gcttccgatt ccggttggtc
     5341 gatggttttc ggttggttcc tttttgattt gctgacggtc ggtatccaat ttttctctca
     5401 tcagaaaatc taccctttcg aatccctgga aaaaatatta tttatccgta tcttcagtat
     5461 ttttttagcc gctgtggcga tagcgatcgg gaattcgatt gaattgaatc ccgcgcaatc
     5521 ctttttggaa gggcaacgcg aaacgattct cctcatcgcc tgcgcgatgc gagccggctt
     5581 attccctttt ttcgaatcgt ttcaaaatga aaacgaaacg agaaagggac tgaacacatt
     5641 tgttgaactg tcatccgtcg ctttggtgtt acctgccttt attcggatcg attcttccgc
     5701 catgggtaat gacgcctacg gcttttttat gtttttttca accttatccg ttatttgcgg
     5761 cgggctcgga atgatttttt caaacaaaaa cgcatccgaa cgccgttatt ttattttgac
     5821 tcttaccggt ttatctttta tttccgcatt gcgattccaa ccgaccgcat ccgtcgtatg
     5881 gggaatatct ttatcggtcc ttaccgcgcc tttgttcctc tatgatatta ggagccggtt
     5941 gttgaatgtc gttcagataa tttccggctt gcttctttca ggacttccgc ggacgccggc
     6001 agcctccggt tggcggggat tggtgctcgt tccaccatca tttttcgact ttttcctgat
     6061 aatctctttc gcctgcctgc ttacgaagtt cttttcggat ttaacggttc aaaatcccgc
     6121 tcaccccgct ctgcaagatc gccggatgaa tgccatttat cctttcggct ttatggtttc
     6181 cgtgatcgcc tacggagtga tcgctgcatt gagttggaat ttttattcgg ataacggaat
     6241 gatcccggca tcgattatta cgctgatcat cgctttcatc atcggatttc gatccgtctg
     6301 gctaagattt ttcactcaga tgaatacctt cttcagctgg tttcgggaaa ttttttttac
     6361 gatttggaaa attttcttat ggatcattca atttcggtgg ttggaagctc ccatcagatg
     6421 gatcataacc ggtttatccg ccattatcga tttcatttcg ggaatttttg aagctccggg
     6481 cggcattctt tggaagttcc tgattctgat tatcgtatta tttctggtta tcggtattta
     6541 aggaacagca tgcgtggtct tttctttacc tcaattatcg gcggcgcgtt gatcatcctc
     6601 ggtgctctca tcatttttat ccgattgaat cagccggaca gccgaatcag tctgatcgct
     6661 tatattctcg agtctgtcgg aatcgttctg ctgttggttc gaattttttc tcctcagatc
     6721 ctgatttccg tcttcctcac cggcacgatg ggagccgcca tactgggaac cgatcaggtt
     6781 gcccttcata tccctctgaa accggataaa gtcaatccgc agggaatttt caggcttctc
     6841 ctcggtttca ttcttgccgt tttgatcctc tcgatcgaac ctgaaatagc tgtttggata
     6901 ccggtgccgg actcgatctt attctgttcc ttgtttctga tattaatggg gctaaccacc
     6961 ttcagcctca gctcccgctt gatgtaccgt ttcttgggaa ttctgaatat tttcagcggc
     7021 tttcaactgg tttatatgct tttggaagaa tccgttctcg tttttgggtt tatgattgcc
     7081 atcgacttgc tgatcgccct attgggggct tattttatga gcggctcaca aaatgacgaa
     7141 tacccgacct gattttgaca ggaaagctta aaaatgaatg cacctctcct ttggtttatt
     7201 atcccctttt tcttcggagc ttttctgctg ctattttcgg aaagcaaaaa attatcgatt
     7261 attcttgcct caattgtctg tctttttctg attgcgattt ccgcaacggt tccgatcaac
     7321 agcatggtga ttatcggaaa aaattcgatc atctttaccg attcaatgga aatactcgga
     7381 agaacattat cgcttgagag agcggcgcag ccccttctcg ctttctttta ttcatatgca
     7441 ttcatctgga ttcttttttc cgctgccgtt cgggttcatc gcttttttgt accgctggtt
     7501 ttgatggaaa ctgctttggt aatcggtgtg acggccgttt cccctttcat ctatggtgct
     7561 ttttttgtcc aattggcggt cttgatcgct ttaccgattt tttggaatga gaaaatgaaa
     7621 tctggcgagg gaattattcg cttcttgctc tatcaaacct tggggttaat ttttttggcg
     7681 atcggaggct ggctggcttc cgcggtggaa ctcaaccctt ccgatcaata tatcttgaaa
     7741 agaacaatgg tcgtccttct ggtcggtttt attttttggt tggcgatttt tccgtttcac
     7801 agttggatcg caatactcat ggacgaaagc tgcccttacc tctcgggttt tgtgattaat
     7861 attttacaat tttccagttt tctgatcctg ctgtattttc ttaaatccta tttgtggctg
     7921 cgaacctatg atccgctttt tattggattg agaatgatcg gcacgatcat gttaatcatc
     7981 ggcagtctct gggctgtttt tcaaaaatcg gttcaacggc tccaggcttt tttaattgtg
     8041 gctgaaaacg ggattgcctt gtttttattg ggactccata caacccaatc cttttcggtt
     8101 tttatttcgt tactgtttat tcggatgatc gcatctttct tctgggcact cgccgtcaaa
     8161 tccctgccgg aaaattttga tttcggattg aattccctgc aggggcttta tcgttctgat
     8221 ccgttcatca gtttgacact catcgtcagt tatttttcaa ccgtcgggtt accgtttctg
     8281 cccggttttc cttatcaagc gacattcctt cagcttgctt tttcagaatc gtcatccatg
     8341 ggaacggttc tttcgatcgg atattggctg ctgattgcgg cgggatttcg gttggcagtg
     8401 acgttgctcc acccggctga gaaagctgcc ggaaaagcgg aaaccgcaaa tcagcgagct
     8461 ttattgatca tcggaatcgc cgcacttttt ttgattactt ttttccctga acccttcaat
     8521 cggtttattg ctcagattca atctcaattt acactgatca gcggcgggta atgatgtcct
     8581 ttccggaaat agtataataa aaccagtcag gagcgaatga attatggcaa aaacttggaa
     8641 cggcggacca ctcaatgaag gatttgttga tctcgaccag cttgtcaaac ggcttgaatg
     8701 gcttgacaat gagcggagaa acgacaaaaa ccgaattctc gccattgaag agcgcctgac
     8761 agacgttgtc agtgaaaacg ttttgcttaa aaatcagatc aaagacctgg aagaagaaat
     8821 tcagagatat gcgaatattc aggcgaaaat cgagcagaat gaatcgttga ttgcgcaatt
     8881 gcggattgat gctttcagag cgatcgaaga aaaagataaa gctcacagcg aaagagaaaa
     8941 agaaatcgat gccgctcgcc gtaacggaat cgattccatt aaccgcactc tggccgacct
     9001 ccgtaaatcg attgaacctc ttcaagatat ccgaaaaaca ttgctttcgc gaaacgagga
     9061 ttccattcgg ttgggcagga tgatcgaaga attgcaagcc aagatcgatg cggttgccat
     9121 ggataaagat gaatatgcca gattccaaaa atccgtggag gaaaacagaa ggcaagacgc
     9181 caaacgggtg actgacattc aagccgaagt caccgcttac cgaaaacgaa tcgaggatat
     9241 gagaagcaaa ttcgatttgg taagtgaagg tttgcggaaa gtagaacagc ggatgagcga
     9301 attattggcg gcggaaaatg aacgaaagca atccgtcact gattttatgg aaaaacaatc
     9361 gttagccaac ctggaatatg aaaagacctg gcagcaatgg aaaaccgcct ttgatgatat
     9421 tgttgccaaa gccgccggat tggatactca aattgagaca ttggagaatt tacagcgttc
     9481 cgtaaaacgc tcgcaggata cgttggacga ggttactcaa cgttttgatc ggcgcaccaa
     9541 cgagctggtt gaaatgcaac gcctcatgga agaaaaactg cgtcaggagt gggcgaattt
     9601 caaggcggat aatcaaaagc gctggaccaa ttattcgtta gggcaagaag aaatacaggc
     9661 ggaatatcga cggaatatcg aagcccttca ggccagaatg caggctatcg aagatgccag
     9721 cgcctcagct ttggacgacg ttaatttaag agatgaactt tatatttcgc aattacaaga
     9781 cttgtttaca atggtacgtg gctggcttga gaaaatgggt aaggcaagct gatcacaact
     9841 ttaatatttt atgattaagg agaatccttc atgagtcaaa tcacagtgat aggaacgggc
     9901 tatgtcggct tggtaacggg agcatgcttt tccgatttgg gaaataatgt catttgtcta
     9961 gatattgatg agaagcgaat ttcggatctc aatcatggca ttatgcccat cttcgaaccg
    10021 ggattggaag aaatcgtcga acgttcacat aaagccggtc gattgcaatt tacggtaaat
    10081 tatccggaag ccctgagaga tgccgaattc gttttcattg ccgtcggaac cccttccgga
    10141 tccgacggag aagcggatat gagctatgtc gagcaggcgg tcatacaaat tgccgacaac
    10201 atggatcatc ccatcattat tgtggataaa tcaaccgttc ccgtcggcac gggagattgg
    10261 gtagcggaca ttattcaaaa ataccgtgac ggtcatatgc cgaaatttca agttgtatcc
    10321 aatccggagt tcctccgtga agggtctgct gttgccgatt ttatgcatcc ggaacggatt
    10381 gttttgggct ccaatgatac cgaagcctgt gatcgggtcg ccgaattgta tgcaccactg
    10441 cgggctccga tgttggttac ggatatccgg acagccgaaa tgattaaata tgcctcaaat
    10501 gcctttttgg caaccaaaat ctcttttatc aacgagattg cgaatatttg cgagagtgtc
    10561 ggtgccaatg tgatcgacgt agccagaggc atggggttgg ataaccgcat cgggtcgaaa
    10621 ttcttaaacg caggaatcgg ttggggagga agttgcttcc cgaaggatgt aaaggcgctt
    10681 gcccatatcg ccgtggaata cgggacacag ccccagctgt tgcaagctgt gatcgatatc
    10741 aatcaaacgc agcgccggcg ggtcgttgaa aaactgaatc ggatgctaaa cggattggaa
    10801 ggaaaggaaa tcggtatttt gggcttggcg tttaagccgg ataccgatga tatgcgcgaa
    10861 agccccgcgg cggatatcat ccgatatctg acgaatgaaa aagcaatcat ccgtgcctat
    10921 gaccccgaag caatggagca ggcaaagaag attctgccga aagaagtcat cctctgcgat
    10981 aacccttatc aagtggttga aaacgcggat gccctccttc tggcgaccga atggaatgaa
    11041 ttcaaacaat tggattttaa aaaaatacat gacctgatgc gcgaaaagaa cattttggac
    11101 ggccggaatc tttgggatcc cgaacgtctt tcggaactgg gctttactta tgtcggcgtc
    11161 ggtttgggaa aatacatttg acgccgattt accatcgatc ataaaaattc gagcataacg
    11221 aaaacagaag cgcattcttt aatgaaatgc gcttctgttt tttataattc gttattctta
    11281 ttgtttatcg ttgtttattc ttgttccgtt tttaatagga aataattctt ctttcctctc
    11341 cggaggacca gatatttccc ttcgataaac tgatctttcc gaatggaagt gttcacatcc
    11401 gtcacccgat gattattaat ataaacaccg ccctcggcga ttgcccgttt gacctctcct
    11461 ttggatttca tgaatccgca atcgctcagc agattgacaa tgggcagccc ttctccgttc
    11521 attgaatccg ccgcaatcac gccggacgga acatcagcga aaatctcttt gatatccgca
    11581 gccgagagac cgttgatttc accgccgaac agcgcttcac tggcttgcat cgctttatct
    11641 aaagcggtct cgccgtgcat catttttgtc atttcaaccg ccaatgcttt ttgggccgtc
    11701 cgatcttccg gattttctcg ggttgcaaat tccaattcgt caattttttg tcgatcaaaa
    11761 aaagtaaaat acttcaggaa agggattacg tcggcatcat cggtattcag ccaaaattga
    11821 taaaatttgt aaggggaagt cctttccgga tcaagccaaa ccgcgccacc ggcagtcttt
    11881 ccgaactttg tgccatcctt tttctttacc aacggatagg tcaatccgta agcattttta
    11941 ccggtttttc ggcggattaa ttcacaaccg gccgtaatat tgccccattg atcactgccg
    12001 ccggtttgca atacacaatt ttcggtcacg aacaagtgat aaaagtcata agcttgtaag
    12061 aggctgtaac tgaattcggt aaaagataag ccgctctccc gagacaagcg cgattttacc
    12121 gagtcttttg ccatcatgct gtttacggtg aaatatttcc cggtatctcg cagaaaatca
    12181 atgagtttca gatcaagcaa ccaatcgccg ttattcataa tgcgagccgg attgcttttt
    12241 atttcaaagt ctaaaaattt cgccagctgc cgcttgattt tctcgacatt gtaatgagct
    12301 tcttccgccg aaagcaggtt gcgttcgtcc gatctgccgc tcggatcacc gatcattccc
    12361 gttccctcgc cggcaagcgc gatcacatga tgaccgtaac gttggaagcg ggctaatgcc
    12421 agcatcggca ccaaatgccc gacatgcaaa ctgtccgcgg taggatcaaa gccgttataa
    12481 agcgtcacct tttccttttc cagtaactct tccaaaccgt ccatcgaatc ataaatcatc
    12541 tcacgccatt ttaattcgcc gtaaatcgaa ttttgctcac tcattgtcgc tataaacccc
    12601 ttcttgattt gtctgttttt atttttaaat agtaaagcga taaatattcg tttcgcgcgg
    12661 ttcaaatccg caacggcggt agaggcggtt cgcagcttcc cgtgacggtc gggaggttaa
    12721 atcgaccgtt tttgcacctt ccgctctggc acgatcgaca gcggctctaa ccaacatttc
    12781 accgacaccc cgcccgcgca tttgctcatc t
//
LOCUS       DAOMIV010000005        12094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_5270_length_12094_cov_4.892765, whole genome shotgun sequence.
ACCESSION   DAOMIV010000005 DAOMIV010000000
VERSION     DAOMIV010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 12094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12094
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5270_length_12094_cov_4.892765"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1856
                     /locus_tag="PKV59_00260"
     CDS             249..1856
                     /locus_tag="PKV59_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQP45210.1"
                     /translation="MSSTGNQPVKGKKKRRKSTIVIWVLIGIIVLMLAAGGYLYQQQK
                     QVAAENASKISSQMTRNVKVEQGLISENLEEMSGTVRSNQSISLNWKISGTVSDVYVQ
                     PGDQVKKGDILAALDPATIDTSILDAAVTKEDAQEQLERLYTSSLDLATAYNTMITAQ
                     DAVKTAEDALSALGVVREDEIEIGVYYQDYLNAKSAYESALDNYNTLKVRPLDDVDRQ
                     RASQRLESARSAMESALSMYNWYNGEVDSLEKQQAEADVILKKAQYDDAVRAYNKIKD
                     GPTESQVKSLEAQVKSAEATVNTAYIIAPIDGTVAEVASKQFDVITLDNTTDEVLAIR
                     LEDLSSYSIDVSVSEMDVNSVEVGQTAEITFDAVPLKMFTGKIVSISNVGDTSGEEVT
                     YALTIRMDDTDPSVRPGMMADIQIRTKEATGALIVPKTAVGTTQDGSQYVEKVNQDGT
                     TTRISVTTGISSDENIQILSDQIQAGDEVRETVLPDMSQNAGATGAGGLFSGMPIRIG
                     GGAAGGGAPREGGQGGPPPDGGGGQRP"
     gene            1873..3051
                     /locus_tag="PKV59_00265"
     CDS             1873..3051
                     /locus_tag="PKV59_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45211.1"
                     /translation="MTEKNGKNVIHTEHLKKYYVMGDIEVRALNDISLDIDYGEFTAI
                     MGPSGSGKSTLMNILGCLDRPTDGIYLLDGIDVSQMDDSELAEIRNRKVGFVFQSFNL
                     LPRSTALENVMLPILYSKNKANAKERAAEALELVGLGSRMKHKPKELSGGQQQRVAVA
                     RALVNDPEIIMADEPTGNLDSKSGGEIMELLIKLNQERGKTIIMVTHDPRIGRHVPRV
                     ISVFDGMLGTEEEQAVIRSWVKPDGEDIPDIKGKSSEKKNDANDKQTNGKNGSNGNGE
                     KHQSENTDKNDPIENKNTNDNKADDPSSDDDPPKNAGHITATEALIRAFGNGKSSPNG
                     GDHSDGDGYHGDHKAGETNGAADALANAFGKQIDEDAIRAAQSSSVKKQPKAGKGGAR
                     "
     gene            3048..4271
                     /locus_tag="PKV59_00270"
     CDS             3048..4271
                     /locus_tag="PKV59_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45212.1"
                     /translation="MNLGTLIHEAITNLLSNKVRSLLTMLGIFIGVGAVIAMMSVGLG
                     AKKSITDQMSALGADTLYISPGNFTQTVRNPGTLTNEDANAIAALDCNDTISPTSSAQ
                     LTVSRAGKQSSTLTVTGVFPTYLTIENYSLAEGRFITQADLDNNAMVTVIGSDTRDLM
                     FETYEEAVGNTVRIGGQPYEVVGVLKSKGDASMISSDRGVYVPLTTAQNRLITRQKHA
                     LDQIAMKINPAYSAEAAEAAVTALMRDRHDILSGEPDDFSIFNMQEIVDTMNSVMNSF
                     VIFLGSVAGISLLVGGIGIMNIMLVTVTERTKEIGLRKALGAKNRDIQLQFLVESSVL
                     SLIGGIFGIIFGYVLAYAVAAIAANAGSPMTPEINPLIIIGTCLFSILTGLFFGIYPA
                     NRAAKLEPVIALRTE"
     gene            4301..5965
                     /locus_tag="PKV59_00275"
     CDS             4301..5965
                     /locus_tag="PKV59_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQP45213.1"
                     /translation="MALLTGLKSSGGLQPKKMLRNLALVLCLFAVIGCSAISQKVGIG
                     STATQAGLEGIPVTRGDISAAVTLVGNVTYAQSAVILWKTTGVVQNVNVKIGDQVKQD
                     DILAELSEDSLNSSVLIAEKNLIDAQDNLTNVMESTTAKSEALSTLTAAEITYQATKQ
                     AQEALYYPRGSQLDVEMAYDAYQLALENYNYAKEDYRVVLANYKGWDDAARTTYYQAY
                     KTAYDNLISTYEDWKYLSGTPDNVELASAQGEVLSAQKAFQDALDTYSSYQTLPRDED
                     LHEANVTVHSAESSFKQRYTIAPFSGTVTAVYAEPGLYVTSGTSAFQLDDMSEYYISL
                     NTSEVDVKDIYAGQKVQITLDAIPNTTFSGIVKQVAGAGTSSTTNTVFKTLIKFDQQD
                     PQIKAGMTAEVNIFLGTKEDVLQVPLSAINQENGRSYVLKINGAETQKIEVTTGVTSG
                     NLIEVNSDELKEGDQVVVSETDLQNYLNSLTETMTTPLSAESSEGTSLAPIGTFQPVG
                     SGTPAAPIGTFQPQKTGMNPGRPAGTRPAFSGTPYPPNMNKTENNG"
     gene            5969..7474
                     /locus_tag="PKV59_00280"
     CDS             5969..7474
                     /locus_tag="PKV59_00280"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQP45214.1"
                     /translation="MDHKITVQTKNRKNTWIILILTLVVIALGIFLYIFYVGQKASQA
                     AVAARIAAGQPSVSSVESGPMKLVIEDISGTVRSNQSVNLAWKTSGTVAAVNVSVGDS
                     VKQGDILAELDPKTLSIQMQNAQVELDDAKDDMEKLTNNAEATTSAVTELVKAQQELD
                     DAQKAYDSLDPSRAWTDAVQLAYETYLKAQNDYESALAKFEVTRDYPIDDATRIKRLG
                     DVGGYRSVRDNALAEYRRYLGEGNEMERLIREAALQLAKATLEEKQYQYDKVTKGPTE
                     AEINAAQAKIDAAQQKLNSSKLIAPFSGVVTQLETKVNDVISETAAASATKTTAIRIE
                     DLSTFYIDTTVNELDVNSIQPGQAAEIQFIGIPDKTYHGTVTTVADTGVSSGRSVTFN
                     VTLKIDDADELVKSGMTADLSMTIANIPETLYVPLSSVLLKNGQPSVNLKKMDGSFEW
                     VPIETGLVSGTKVQVLSDNLKAGDEIEADVNVDSTDTNDNRMFPMGFGGRM"
     gene            7661..8479
                     /locus_tag="PKV59_00285"
     CDS             7661..8479
                     /locus_tag="PKV59_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HQP45215.1"
                     /translation="MKILIACDMEGITGVTRWEHCDPTHPEYQRFRKQMTMDVNAAVE
                     GAFAGGVEEVQIADGHSQGQNLLIEELDSRAQLNCGTTSPWSMMNGIDSAFGGVFFIG
                     YHAMAGTANAILAHTWSTQILNWKINGQKIGEFGMNALIAGWFDVPALMVSGDQSLAM
                     EALRFVPDIDCAVVKEANGYFSGKCFSPAKTHERILECAQLAVQKKREGRGPQPLKMN
                     APYEVEVEFTQPYMAETASRLTGSERTGGRSVTITVPDIPAAFLAARTLCNLAV"
     gene            8540..9436
                     /locus_tag="PKV59_00290"
     CDS             8540..9436
                     /locus_tag="PKV59_00290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQP45216.1"
                     /translation="MKYFSDTSNPEQNNRKTYQALIITLAGNLILALSKLYVAHVSGS
                     SALQADAYNSLSDVLYSVTLVIGMMIAIRPADISHPQGHRRFEPLVGLLISFSMAWAG
                     YQAISESIDKIRSGPTTFDLGLPILVLFFSAVIKGVMYWLIHRIAIDLSSPTLKTAAT
                     DNLMDTITSFTAIIGIYLSSIWSPLADPIAGILVSVWIFRAAFEALQENLGFLTGAGA
                     SEERRQQFLDIIRSVPGVANVHQLFAEHVGSTFVLDVHINVDGNTPLYQVHRIETEIE
                     EKLTAIPDVERAYIHVEPLGYE"
     gene            9612..10049
                     /gene="dtd"
                     /locus_tag="PKV59_00295"
     CDS             9612..10049
                     /gene="dtd"
                     /locus_tag="PKV59_00295"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQP45217.1"
                     /translation="MKAVIQRVSRASVTVDGRLISRIGTGLLVLLGVEEGDTRENALA
                     MAKKTAALRIFRDENDKMNLSVRDCAGEAIVVSQFTLCADTRKGNRPSFIGAAAPEIA
                     APLVDLFAESMREQGVPTQQGLFGADMKVELLNDGPVTILLEN"
     gene            10052..11914
                     /gene="uvrC"
                     /locus_tag="PKV59_00300"
     CDS             10052..11914
                     /gene="uvrC"
                     /locus_tag="PKV59_00300"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQP45218.1"
                     /translation="MEISPKIQGILETLPNKPGCYLMKNADDKIIYVGKAINLRHRVR
                     SYFQNSVKHTVKTRQMVSHIDHIDWIVVESELEALILEMNLIKKNRPQYNIQLKDDKR
                     YPYIKIEFQNPYPRILVTRQMVQDGSRYFGPYTSVWAVHQTLDLLRRIFPYLTCDREI
                     TGQDSRPCLYYDIKLCSGPCINAISQADYYAMMEDIASFLEGKTADIVNRLQKQMSDA
                     AENLNFERAAALRDQIQAIDRVVEKQKIVSNDDTDSDVIAIARSEKEACIQLFYIRNG
                     KLIGQEYFIMKGTEDEADQEVVSEFIKQFYSDAATIPPQMLLPVELEEHRIIQQWLRQ
                     QRGGKKFTLKVPKTGNEKDLVNMAAENAIETLRALKTQWENDTNKQSEALTELQSALQ
                     LPEPPNRIECYDISNTQGTLSVGSMVVFEQGVPKKNLYRRFNIKTVEGPNDFDSMTEV
                     LTRRMKRWQSASEKDNKTGGKIDPSFSVLPDLIIIDGGKGQLGRAVEVLKDFDLLDRV
                     HVVGLAKREEEIFMPFQSDPIILPRNSEGLYLVQRIRDEAHRFAITAHRGRRTKEGLA
                     SQLEKIPGIGPVKRKALLKKFGSIDAIAAASKEELMLTDGITSSLADAIRDYFG"
BASE COUNT         3398 a         2562 c         3175 g         2959 t
ORIGIN      
        1 cgagctctgc gattcgggaa gcggataacg gttcctatgt tgacgttgtg gtgaacggga
       61 cactgacgcc tgtttccgtc gttacgggtg tggaagaaaa cgggatggtt gaaattgtct
      121 ccggaaatct caacgaagga gatttggtaa aagttccttc agcgtaattg gggattcgaa
      181 cagaaatttt aaatgaatga agaagtgaaa gattattcta aacaaacagt gaagaacagg
      241 aatgaattat gagttcaacg ggtaatcaac cggtcaaagg gaaaaagaaa agaagaaaat
      301 caacaattgt gatttgggta ttaattggaa tcatcgtttt gatgctggct gccggcggtt
      361 atttatatca gcaacaaaaa caggtcgccg cggaaaatgc atccaaaatt tcgagtcaaa
      421 tgactcggaa tgtcaaagtg gaacaagggt tgatttcaga gaatctggaa gaaatgtcgg
      481 gtacggttcg atcgaaccaa tcgatcagtc tgaattggaa aatcagcggg acggtatcgg
      541 atgtttatgt tcaacccggt gatcaggtga aaaagggcga cattctggcg gctttagatc
      601 cggccacaat cgatacttcc atactggatg ccgctgtcac aaaagaagat gcgcaagaac
      661 agttggaacg cctctatacc tcttcactgg atctcgctac agcctataat acaatgatta
      721 ccgctcaaga tgctgtgaaa acggctgaag atgctctttc ggcattgggt gtcgttcgtg
      781 aagatgaaat agaaatcggc gtttactacc aggattatct gaacgcaaaa tccgcttatg
      841 aaagcgcgtt ggataactat aatacgctga aagttcgacc cttagatgat gtcgaccggc
      901 aacgggcttc ccaaagattg gaaagtgccc gttcggcgat ggagagtgca ctttccatgt
      961 ataactggta caacggtgag gtcgacagcc tggaaaaaca acaggcggaa gcggatgtga
     1021 tcttgaaaaa agcacaatat gatgacgcgg tccgcgctta caacaagatc aaggacggtc
     1081 cgacggaatc ccaggtcaaa tctctggaag cacaagtaaa atccgcggaa gcgacggtta
     1141 atacagctta tattattgcc ccgattgatg ggacggtggc tgaagtggct tccaagcaat
     1201 ttgacgtaat tacattggac aatacaacgg atgaagtttt agccatccgc ctggaagatt
     1261 tatcctccta ttctattgat gtgtctgttt cggaaatgga tgtgaactcg gttgaagtag
     1321 ggcagaccgc cgagatcacc tttgatgccg ttcctttgaa aatgtttacc ggaaagatcg
     1381 tcagcatttc caatgtcggg gatacgagcg gagaagaagt aacttatgct cttacgattc
     1441 gaatggacga taccgaccct tcggttcgtc cggggatgat ggcggatatt caaattcgca
     1501 cgaaagaagc taccggtgct ttgattgttc cgaagacagc tgtcggaacg acgcaggacg
     1561 gtagtcaata tgtggaaaaa gtcaatcagg atggcacgac aacccgtatt tcggttacaa
     1621 ccggaatttc ttcggacgag aacattcaga ttctttccga tcaaatccaa gcaggtgatg
     1681 aggtaaggga aaccgtttta ccggatatgt cgcaaaatgc cggagcaacc ggtgccggcg
     1741 gactttttag tgggatgccg atccggattg gcggcggtgc tgccggaggc ggtgcgcctc
     1801 gcgaaggagg tcaaggcgga ccgcctcccg acggtggcgg cggacagagg ccatgaaatt
     1861 gctgagggaa aaatgacaga aaaaaacgga aaaaatgtaa ttcatacgga acatttaaag
     1921 aaatattatg tcatgggcga tatcgaagtc cgtgctttga atgatatctc gttggatatt
     1981 gattatgggg agtttacagc cattatggga ccatccggct ccggaaaatc aactttaatg
     2041 aatattttgg gctgtttgga tcgtccgacg gacgggattt atttgctgga cgggatcgac
     2101 gtatcgcaaa tggatgattc ggaactggct gaaattcgta accgaaaagt cgggtttgtt
     2161 ttccaaagct tcaatttgct tccgcgttcc acggcacttg agaatgtgat gctgccgatt
     2221 ctctattcaa agaataaagc aaatgcgaaa gaacgggcgg cagaagccct ggaattggtg
     2281 gggctgggca gccggatgaa acataaaccg aaggagctat ccggcggaca gcaacaacgg
     2341 gtggctgttg ccagggcgtt ggtaaatgat ccggaaatca tcatggcgga tgaaccgacg
     2401 gggaacctgg acagtaaatc cggcggcgaa attatggaat tgttgattaa actgaatcag
     2461 gaacgcggaa agaccatcat catggtgacg catgatccgc gtatcgggcg gcatgtcccg
     2521 cgcgtgatca gtgtgtttga cggaatgttg ggaacggagg aggaacaggc ggttatccgc
     2581 agctgggtga aaccggatgg ggaagatatt ccggatatta agggaaaatc ctccgaaaag
     2641 aaaaatgatg caaacgataa acagacaaac gggaaaaacg gatcgaacgg caacggagaa
     2701 aaacatcaat ccgaaaatac ggataaaaac gacccgattg aaaataagaa tacgaatgac
     2761 aacaaggcgg atgacccgtc ttcggatgat gatccgccga aaaatgccgg tcatataacc
     2821 gctaccgaag cattaatcag agctttcggg aacgggaaat cttctccaaa cggcggtgac
     2881 cattccgacg gcgacggtta tcatggggat cataaagcag gagaaacaaa cggagctgca
     2941 gatgctttgg cgaatgcgtt cggaaaacaa attgacgaag atgcgatccg agcggcacaa
     3001 tcttcatccg tgaagaaaca acctaaagcg ggaaagggtg gcgcgagatg aatctgggaa
     3061 cactgatcca tgaggcaatc actaacctgc tttccaataa agttcgctcg ttactgacta
     3121 tgttgggcat ttttatcggt gtcggtgccg ttatcgcgat gatgtcagtg ggattgggcg
     3181 cgaagaaatc cattaccgac caaatgagcg ccttgggagc ggatacgctt tatatttctc
     3241 cgggaaattt tacccaaacc gttcgaaacc cgggaacgct tacgaacgaa gacgcaaacg
     3301 caatcgctgc gttagactgc aatgatacca tctctccgac ttcttccgct caattgacgg
     3361 tttcccgagc ggggaaacaa tccagtacct tgaccgttac cggcgttttc ccgacttact
     3421 tgacgatcga aaactacagt ttagccgaag gcagatttat cactcaggct gatttggata
     3481 ataatgccat ggtgactgtt atcggttccg atactcggga tctgatgttt gaaacctatg
     3541 aagaggcggt cggaaatact gtccgaatcg gcggccaacc ctatgaagtg gtcggcgttc
     3601 tgaaatcgaa aggggacgcg agtatgatca gttcggatcg cggggtttac gttcctttga
     3661 caaccgctca gaatcgcctg ataacccggc agaaacatgc cttggatcag attgctatga
     3721 aaatcaatcc ggcgtacagt gcggaagccg ccgaagctgc cgtgaccgca ctgatgcggg
     3781 atcggcatga tatcctttcc ggcgaaccgg atgatttctc gatctttaat atgcaggaaa
     3841 tcgtcgatac catgaattcc gtcatgaatt cctttgtgat tttcctcgga agtgtcgccg
     3901 gaatttcgtt gttggtaggt ggaatcggta ttatgaacat tatgttggtt accgttacgg
     3961 agcggacgaa agaaatcggc ttgcgaaaag cattgggtgc gaaaaatcgg gatattcaac
     4021 ttcaattttt ggttgaatct tccgtactga gtttgatcgg cggaatattc gggatcattt
     4081 tcggttatgt attggcatat gccgttgcgg cgattgcggc aaatgccgga tctccgatga
     4141 cacccgaaat caaccccttg attattatcg gaacctgtct gttctcgatc ctgaccggac
     4201 tgtttttcgg aatttatccg gcgaaccgag ccgcaaaact ggaaccggtg attgcgttac
     4261 gaacggaatg atgacggtga aaaacatggt tgggagaaaa atggctctgc ttaccggact
     4321 gaaatcatcc ggtggcttac aaccgaaaaa aatgctgcgg aatttggcac tggtgctctg
     4381 tctgtttgcc gttataggat gctcagctat ttcgcagaag gtcggtatcg gatcgacggc
     4441 aactcaggcg ggattggaag gaataccggt aacccgcggg gacatcagcg ccgccgttac
     4501 gctggtggga aacgtgactt atgctcaatc cgcggttatt ttgtggaaga caaccggtgt
     4561 ggtacaaaac gtgaatgtta agatcggcga tcaagtgaaa caagatgata tcttggctga
     4621 attatctgag gattcgttga attcctctgt gctgattgcc gaaaaaaatc tgatagatgc
     4681 tcaggataat ctgacaaatg taatggaatc gacaacggca aaaagcgaag ccttgtccac
     4741 gcttacggct gcggaaatca cttaccaagc cacaaagcaa gcgcaggagg cgttatatta
     4801 tccgcgagga tcgcaactgg atgtcgaaat ggcttatgat gcgtatcagt tggctttgga
     4861 aaactacaac tatgcaaagg aagattatcg ggtggtgctg gcaaactata aaggatggga
     4921 tgacgcggcc agaaccactt actatcaagc atataaaacc gcttatgaca atttgatttc
     4981 aacctatgag gattggaaat acctgagcgg gacgcccgat aatgttgagt tggcatccgc
     5041 ccaaggtgag gtgttgagcg ctcaaaaagc ttttcaggat gcgttggata cttacagttc
     5101 ttatcagaca cttccgagag acgaagatct gcacgaggcc aacgtgacgg ttcattcggc
     5161 ggaatccagt tttaagcaac gatatacgat tgcgcccttt agcggaaccg ttacagccgt
     5221 ttatgcggag ccgggtttgt atgtcacttc cggtacctcg gcttttcagc tggatgatat
     5281 gagtgagtat tacatttcgt tgaatacttc ggaagtggat gtcaaggaca tttatgcggg
     5341 acagaaggtg caaattactt tggacgcgat tccaaatacc acattctcgg gaattgttaa
     5401 acaggttgcc ggtgccggaa cttcatcgac taccaatacc gtgtttaaaa cgctgattaa
     5461 atttgatcaa caggatccgc aaatcaaagc gggtatgact gcggaagtga atattttcct
     5521 cggaacgaaa gaggatgttc tgcaggttcc tttaagtgcg atcaatcagg agaacgggcg
     5581 ttcttatgtc ctgaaaataa atggtgccga gacccaaaaa attgaggtca caaccggtgt
     5641 cacatccggt aacctgattg aagttaattc ggatgagctc aaagaagggg atcaggttgt
     5701 tgtttcggaa acggatttac agaattattt gaattcgctg acggaaacga tgacgactcc
     5761 actatctgcg gagtcgtcgg aaggcacctc tttggcaccg atcggaactt ttcagccggt
     5821 ggggtcgggt acgcctgcgg ctccgatcgg aacttttcaa ccgcagaaaa ccggtatgaa
     5881 ccccggccgc ccggcaggta ctcggccggc tttttccggg acaccgtatc caccaaatat
     5941 gaacaaaaca gaaaataacg gatgaaaaat ggatcataaa ataacggttc aaacaaaaaa
     6001 tcgaaaaaat acctggatca ttctcattct gacacttgtc gtcattgctc tgggtatatt
     6061 tctttacatt ttttatgtgg gtcaaaaagc cagccaggct gcagtagcgg ctcggatcgc
     6121 cgccggtcaa cccagtgttt cttcggttga atccggtccg atgaaattgg tcatcgagga
     6181 tatcagcgga acggtacgtt ccaatcaatc cgtaaatctt gcctggaaaa caagcggaac
     6241 cgtcgcagcg gtcaatgtat ccgtcggtga ttcggtaaaa cagggtgata tattggcgga
     6301 gcttgaccca aaaacgcttt cgattcaaat gcagaacgct caggttgagt tggatgacgc
     6361 caaggatgat atggaaaaat taaccaataa tgcggaagcg accacctcgg ccgtaaccga
     6421 attggtaaaa gcacaacagg aattggatga tgctcagaaa gcgtatgaca gcctggatcc
     6481 ttcccgcgca tggacggatg ccgttcaact ggcatacgaa acctatttga aagctcaaaa
     6541 tgattatgaa tccgcattgg caaaatttga agtcactcgc gattatccga tagatgatgc
     6601 cactcgtatt aaaagattgg gcgatgtcgg cggctatcgc agtgtccgcg ataatgcgct
     6661 ggcggaatat cgtcgctatt tgggagaagg aaatgaaatg gagcggctga ttcgggaagc
     6721 tgcgttgcag cttgccaaag cgactctcga agaaaaacaa taccaatatg ataaagtgac
     6781 caaaggcccg accgaagccg aaatcaatgc ggctcaggca aaaatcgatg cggcacagca
     6841 gaaactcaat tcttccaaac tgatcgcacc gttcagcggg gtggtaacac aattggaaac
     6901 taaggtgaat gatgtcattt cggaaacggc cgcagcttcc gcaaccaaaa caaccgcaat
     6961 ccgaattgaa gatttgtcca ctttttatat tgatacgaca gtcaatgaac tggatgtaaa
     7021 ttccatccaa ccggggcagg cggcggagat tcaatttatt ggaattcccg ataagactta
     7081 tcatgggacg gtgacgacgg ttgccgatac gggtgtatct tccggtcgat cggtcacttt
     7141 taatgtgacg ctgaaaattg atgatgcgga cgaactcgtg aagtccggaa tgaccgccga
     7201 tttatcgatg accattgcga atatccccga aacgctttat gtcccattgt cttccgtact
     7261 gttaaagaac ggtcagccgt ctgtaaatct aaaaaagatg gatggatctt ttgaatgggt
     7321 tcccatcgaa accggcttgg tttccggaac caaagtgcaa gtattatcgg ataacctgaa
     7381 agccggtgat gagattgagg cggatgtgaa tgtcgattcg accgatacga acgataatcg
     7441 gatgttcccg atgggttttg gcggtagaat gtaattcgga tcatatcggg atttcttaaa
     7501 tacgacgata gttgatgaaa tgactggggt cggagcgtca ctccggctcc ggtcattttg
     7561 tgacaacaga taagtggtaa aatgaaatga atttccggca ttactatttc tctgcgggga
     7621 ttttttttat atctgtcaac aaaaaaggga tgatgaacga atgaaaatat tgattgcttg
     7681 tgatatggaa ggaattaccg gtgtgacacg ctgggagcat tgcgatccga ctcatccgga
     7741 atatcagcgt ttccgtaaac aaatgacgat ggatgtcaat gcggctgttg aaggcgcttt
     7801 tgccggcggt gtcgaggaag tgcagattgc ggacggtcat tcccaaggac agaatctgtt
     7861 gatagaagaa cttgattctc gtgcccaatt gaattgcggg actacctccc cgtggagcat
     7921 gatgaacgga atcgattccg cattcggcgg agtgtttttt atcggatatc acgccatggc
     7981 agggacggcg aatgccatct tggcgcacac ttggtccact caaatcctta attggaaaat
     8041 caacggacag aagatcggtg aattcgggat gaacgcattg attgccggct ggttcgatgt
     8101 accggctttg atggtcagtg gagatcagag tctggctatg gaagctttgc ggttcgttcc
     8161 ggatatagac tgtgctgttg tcaaagaagc caacggttat ttttcgggga aatgcttttc
     8221 tccggcaaag actcatgagc gcattctgga atgtgcccaa ttggctgtgc aaaagaaacg
     8281 agagggacgg ggacctcaac cactgaagat gaatgctcca tacgaagtgg aagtggaatt
     8341 cacccaaccc tatatggctg agacagcctc ccgattgacg ggatcggaac gtaccggcgg
     8401 tcggtcggta actatcacag tgccggatat tccggcagcc tttttggcgg caaggacctt
     8461 atgtaatctc gcggtgtaac aatttaatac tgcttcgtga agggcgaccg aaaaagcaga
     8521 cctataaaaa ggactttata tgaaatactt ttcggatacc tcgaatcccg aacaaaacaa
     8581 caggaaaacc taccaagcat taattatcac gctggcaggc aatttaatat tggctttatc
     8641 caaactttat gttgcccatg tatccggcag ctcagcactt caagcggatg cttacaactc
     8701 tctttccgac gtcttgtact ccgtaacatt ggtgatcggg atgatgatcg ccattcgtcc
     8761 ggccgatatc tctcatccac agggtcatcg gcgtttcgaa ccgttggtgg gactgttgat
     8821 cagcttttcc atggcatggg ccggctatca ggctatttcc gaatcaatcg ataaaattcg
     8881 aagcggcccg acgacttttg atttgggatt accgatcttg gtgctgtttt tctcggcggt
     8941 aatcaaagga gtgatgtatt ggttgattca tcggattgcg atcgatcttt ccagcccgac
     9001 attgaagaca gccgcaaccg ataacctgat ggatacgatc acatcattta ccgccattat
     9061 cggaatttat ctttcaagca tttggtcgcc attggctgac ccgatagcgg gtatcctggt
     9121 gtccgtatgg atctttcgcg ccgcttttga agcccttcag gaaaatttgg gctttttgac
     9181 cggagccggt gcatccgaag aacgccggca gcagtttttg gatattatcc gttcggtacc
     9241 gggagtggcg aatgttcacc agctgtttgc cgaacatgtc ggatcaactt ttgtactgga
     9301 cgtgcatatc aatgttgatg gaaacacacc gctttatcag gttcatcgga tcgaaaccga
     9361 aatcgaagaa aagctgactg cgattccgga tgtggaacga gcttatattc atgtcgaacc
     9421 gttgggatat gaatagcccc agactgtaat accggcaacg atattttatt cccgctctga
     9481 agaagcgatc cgaatttgaa agaaggatcc gaaacgcatc atttatggac aggaagatcc
     9541 cgatcgattg acttttatca acaaaattct tagaatctta tgataaaaat aattaggcgg
     9601 aatttgaaaa gatgaaagcg gttatacagc gtgtcagtcg ggcttcggtc accgtcgatg
     9661 ggcggttaat cagccgtatc gggaccggtt tgctggttct tttaggagtt gaagaaggag
     9721 atacccgaga aaatgcgctt gcgatggcaa agaaaactgc cgcattgcga atatttcggg
     9781 atgaaaacga taaaatgaac ctttctgttc gggattgcgc cggggaagcg attgttgttt
     9841 cccaattcac cttatgtgcg gatacacgga aggggaatcg cccttctttc atcggggccg
     9901 ctgcaccgga aatcgccgct ccgctggtgg atctgttcgc ggagtcgatg cgagaacagg
     9961 gtgtcccgac acagcagggt cttttcgggg cggatatgaa agtcgaactc ttaaatgacg
    10021 gaccggtgac catcctgctg gaaaattagt tatggaaatc agtccgaaaa ttcagggaat
    10081 tttagagacg ctgccgaata aacccggttg ttatctgatg aaaaacgccg atgacaaaat
    10141 catttatgtc ggtaaagcga tcaatctgcg tcaccgggtc cgttcctatt ttcaaaatag
    10201 tgtgaagcat acggtaaaaa cccgccagat ggtcagtcat attgatcata tcgactggat
    10261 tgtggtggaa tccgagctgg aagcgctgat tcttgaaatg aatctgatta aaaagaatcg
    10321 gccgcaatac aatattcagc ttaaagacga taaacgctat ccttatatta aaatcgaatt
    10381 tcaaaatccg tacccgcgga ttctggttac ccgccaaatg gttcaggatg ggtcgcgata
    10441 tttcggcccg tatacgagtg tttgggcggt gcatcaaaca ttggatttgc tgcggcgtat
    10501 cttcccttat ttgacctgtg atcgggagat caccggtcag gacagtcgac cttgcctgta
    10561 ctatgatata aaactttgtt ccggtccttg tatcaatgca atctcgcaag cggattatta
    10621 tgcgatgatg gaagatattg cctcttttct ggaggggaaa accgccgaca ttgtaaaccg
    10681 gctccaaaag cagatgagcg atgccgcgga gaatttgaac tttgaacggg ctgccgccct
    10741 gcgagatcaa attcaggcta ttgatcgggt agtcgaaaag cagaaaatcg tgagtaatga
    10801 tgataccgat tcggatgtga tagccattgc gcgctcggaa aaagaagcct gtattcagct
    10861 gttctatatt cgaaacggga aattgatcgg ccaggaatat ttcattatga aagggacgga
    10921 ggatgaagcc gatcaggaag tggtttccga atttatcaag caattttaca gcgatgcggc
    10981 gacgattccg cctcaaatgc tgcttcccgt tgagttggaa gaacatcgca ttatccaaca
    11041 atggctgcgt caacaaagag gaggcaaaaa atttacgctc aaagtgccca aaaccggtaa
    11101 cgaaaaggac ctggtgaata tggctgccga aaatgcaatc gaaacgcttc gggcgctcaa
    11161 aacgcaatgg gagaatgata ccaacaaaca aagcgaagca ttgactgaac tgcaatcggc
    11221 actgcagctg cccgaaccgc cgaatcgaat cgaatgttat gatatttcca acacacaagg
    11281 aacgttgtcg gtcggatcca tggttgtttt tgagcaggga gttccgaaga aaaacttgta
    11341 tcgacgtttt aatatcaaaa cagtcgaagg accgaacgat tttgacagta tgacggaagt
    11401 acttacccgc cggatgaagc gttggcaatc cgcgagcgag aaggataaca aaacaggggg
    11461 caaaatcgat ccctcatttt ctgttctgcc cgatttgatc atcatcgacg gcggaaaagg
    11521 ccagctgggg cgcgcggtcg aagtgctcaa ggatttcgat ctgcttgatc gcgttcacgt
    11581 cgtcggtctt gccaaacgcg aagaagaaat atttatgcct tttcaatccg atccgataat
    11641 ccttccgcgt aattccgaag gactctatct ggttcagcgc attcgggatg aagctcatcg
    11701 ttttgccatc accgctcatc gcggaagacg aacgaaggag gggctggctt ctcaactcga
    11761 gaaaatcccg ggtatcgggc cggttaaacg gaaagcgttg ctgaaaaaat tcggcagtat
    11821 cgatgccatc gccgcagcat ccaaggaaga actgatgttg acggacggta tcacttcctc
    11881 tttggcggat gcaattcgcg attatttcgg atgaagaacg gtgactgcgc ttttttacgc
    11941 ctcgaatgaa agtataatta attgatagca atattctcaa ttaaacaaga aagggattga
    12001 tgatggaaaa aactgcggaa caaattcgct atgataaatt gggtgaaacg gttgccaagg
    12061 cgttgcgcaa gaggttcttt gatgcctggt attt
//
LOCUS       DAOMIV010000006        11810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_5426_length_11810_cov_4.045172, whole genome shotgun sequence.
ACCESSION   DAOMIV010000006 DAOMIV010000000
VERSION     DAOMIV010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11810
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5426_length_11810_cov_4.045172"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKV59_00305"
     CDS             <1..989
                     /locus_tag="PKV59_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQP45219.1"
                     /translation="FYLNPKKREENDVMMRRILFEKFGEFGFGEENPKPRPIAGSMMV
                     AGGFVIPALLGAEIRFSTAEAPQPLPQIRSKDQIMSFVEPDILSNWPMNELADGWDRQ
                     QKEYGYLLGDINTDGILNAAYHFYGPDLFADLLEDPDVPVRFMRKIAKIIIQTATYIK
                     NRTGTTSVSVNRMACRLSPSPFIHANCSVQMISPRTYEKNLLPIEQDMAKFISDYGVH
                     HCGVKTEIYAKFYNQIPVCYCEIGWESNPEKCRSFLPNAFLNLRLSPIRMLSCSSSEI
                     KNDVEELLNSVGDLTNVGVSCINMDFGTPEENILTVSETIERYRTLWKEHHG"
     gene            982..2019
                     /locus_tag="PKV59_00310"
     CDS             982..2019
                     /locus_tag="PKV59_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQP45220.1"
                     /translation="MDKLIQNFFQASCHQEVSKVPLALIADSPWIPGFAGIDTLDYYL
                     DQKKWFEINLNLRERFPEVVWVPGFWWEYGMAIEASAFGSRIIFHHDQPPSMEPISTD
                     LQFWKNKTKVRDPEKDGLMPLALRQIERSDHELQSYGLGQHIASSRGILTVTSWFMGV
                     TTLMESLLSAPDDIIPILEVVTDTIILWLEAQLKRMREPKGILLLDDLIGMISKRVYL
                     RIVDPLFRKIWDHFDGYVKIYHNDTSCEHLYPVLAETGFDVFNFSDKTDIGTARQLMG
                     KQIVLMGNVAPRDIGALGTPEQVYQAAWNCLEKTNGEGLILSFGGGISPGTPAENIDS
                     MIKALNDWYKR"
     gene            2068..3492
                     /gene="uxaC"
                     /locus_tag="PKV59_00315"
     CDS             2068..3492
                     /gene="uxaC"
                     /locus_tag="PKV59_00315"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF002794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HQP45221.1"
                     /translation="MSKKWNLPEDRFFDNEPAQKKIALEIYERIKNLPIVSPHGHVDP
                     KLFANDSPGFGTPSDLLVIPDHYIFRLFYSQGLKLEDFGIPYRNGLQDASEKDHRKIW
                     QHFADNFYLLRTTASYTWLAYEFKFIFDIDEIPDSENAMRIYDNLTEKLSSPEFQPRE
                     LYKKMNIEVLCTTDAASDSLKTHQEIKESGWDGKIIPTFRPDAVTNIRGENFRQEIEK
                     LSAAADIDIHDFESYIRALEKRRAFFKEMGAVSTDQAVITPYTHELPQSETDRIFKAA
                     LNGNVSDSDATAFEGHMLIEMARMSCNDQLVMQLHPGVLRNHNRIIYDKFGPDKGCDI
                     PIQTEFTKNLHSMLNQYGNNPNFTLCLFTVDENTYSRELAPLAGHYPSVRLGPPWWFN
                     DSWNGMTRFFDSVTETAGLYNLSGFVDDTRAFPSIPARHDVWRRVVSNWLAKMVVRSF
                     IDVDDAYDFAFLLAYSQPKNVYKL"
     gene            3517..4938
                     /locus_tag="PKV59_00320"
     CDS             3517..4938
                     /locus_tag="PKV59_00320"
                     /inference="COORDINATES: protein motif:HMM:NF027582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HQP45222.1"
                     /translation="MKYSLINTSVTTIDSQKIWMIRDAEGNRYLQSDRPIKALKSENN
                     DNIYLLSPENAKWLRSQIPWLNPQPLGLKTSVGTGDRLGISTPGHIRAFQGTQISPVL
                     AQQSVRELIRTHRTAQMVLDDAMWGVFEAGWNRPWGADADHLKTIDDMREYYEAGFTF
                     FTIDPGEYVKNLNSLDNIPLQSLLNSIDYDLLATSESELINNYTHFPDSLDFITASEQ
                     DIKCAFIKYGGAIHHVQKMYFQLKSWMGNKPFDFEVSVDETDEPTTIFEHYFIACELK
                     RLGIEWTSLAPRFVGKFEKGVDYQGDLHEFESEYRKHAAIQKYFGNYKISIHSGSDKF
                     SIYKICAVNSEYKTHLKTAGTSYLEALRIIATVDPELFRKIYDLSMNRYEIDKASYYV
                     SAHPENLPKISSLADDRLPDLLNQFDSRQVLHVCFGAVLDQYGSDILHDLNRNYDLYL
                     DDLEKHLRKHLQDFIYSEPAEES"
     gene            4946..6217
                     /locus_tag="PKV59_00325"
     CDS             4946..6217
                     /locus_tag="PKV59_00325"
                     /inference="COORDINATES: protein motif:HMM:NF021387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HQP45223.1"
                     /translation="MTFSNEVFGKGSPDPKDFLTESDIKKILTDFFSANNLSGKKVLV
                     IIPDSTRTMPLPLFFKLFYELIHDEVKTLDFMIALGTHPPMSDEAIADEIGISIEEMK
                     GKYQDISFFNHHWDDPKTFIELGEFPKNEVIELTKGTVQRAIPIQINKIILNYDALIV
                     LGPVFPHEVMGFSGGSKYFFPGISGPDLTNTTHWIGALHTCIKTIGLKITPQRQLIER
                     AANLIPVPRLYCCPVVKKEGIFGFYAGEPYQTWEKAVDLSSQVHVKYVDKPFKKVISV
                     MPHLYDDIWTAGKGMYKLEPVVEDGGELIIYAPHITEISYTHGKTLDRIGYHCLDYFL
                     KQWEKFKDEPWGILAHSTHVTGLGTYENGIEKKRIKVSLATGIPKERCERVDLGYVNP
                     QELNLQEYEHKEHEGILLVPYAGETLYRLKQ"
     gene            6272..7090
                     /locus_tag="PKV59_00330"
     CDS             6272..7090
                     /locus_tag="PKV59_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQP45224.1"
                     /translation="MLPDFSVKGKVVVITGGGGAICGMMSKMFGEAGAKVAVMDISMD
                     AAKKITDEINNKGGESIPLVCNVLDKKSIEDAAAQVMGKWGRVDILINGAGGNKPQAT
                     TNNEMSFFDLPADAIQWVFNLNCLGTIIASQVFGKIMAEQKEGCILNISSMNAFRPLT
                     RIPAYSAAKAAINNFTQWLAVDLAKNFSPNIRVNAIAPGFFVGNQNRDLLIDKNSGDL
                     TQRGKTIISLTPMNRFGEFEDLLGATLFLVSPASSFITGVVLPIDGGFSSFSGV"
     gene            7119..8015
                     /locus_tag="PKV59_00335"
     CDS             7119..8015
                     /locus_tag="PKV59_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQP45225.1"
                     /translation="MSELSNQAKELLNFKPKHEFFVGIDSDGCVFDSMEIKHKECFCP
                     NTIRVWGLQPISKYAREAVEFVNLYSKWRGINRWPALIKVFELLSERPEVIRRGAVIP
                     KGDDIRAFINSGLPLSDAGLKKYMETHSSEELDRALEWTTSVNRTVKEIVRGLKPFPY
                     AKESLQAIHPFTDAFCVSATPADALEREWKDGEIRDYVEMICGQEIGTKEYILRLGSA
                     GKYPSDKKLMIGDAPGDLEAAQAVGALFYPINPGHEEESWERFYKEGYKRFLEGTYSG
                     EYEKRLIEDFEKLLPEIPPWKR"
     gene            8069..9481
                     /gene="gndA"
                     /locus_tag="PKV59_00340"
     CDS             8069..9481
                     /gene="gndA"
                     /locus_tag="PKV59_00340"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HQP45226.1"
                     /translation="MEKAKIGLIGLGVMGANLSLNIERNGFPVAVYNRTVEETHNFIK
                     GAAAGKKIYGVETLKDLVDILERPRKIILMVKAGSPVDETIEAIKPYLEKGDILIDAG
                     NSFFQDTERRSEMLAKDGINFFGMGVSGGEEGALWGPSIMPGGQRECWEELRPVLTAI
                     AAKYKDGEPCVAYIGPRGSGHYVKMVHNGIEYGDMQLIAETYDLLHRGLGLGQQRLQE
                     IFSNWNSGVLKSYLIEITAKILSKIDSETGKPLVDVILDEAQQKGTGKWTAQNALDVG
                     APTPTLTSGVEGRILSSLKEERVVASKILKGPDLTFYGDREEIIRAAENALYCSKIAS
                     YAQGLGLLKIAGKEYDYNLNLGEIAKIWREGCIIRADFLNDITDAFNKNPSLQNLMLD
                     DFFRSAIETRQSDWRLIAKTAIDLGIPMLATNASLAYYDSYRSETLPANLTQAQRDFF
                     GAHTYRRIDKEGSFHTIWEE"
     gene            9483..10766
                     /locus_tag="PKV59_00345"
     CDS             9483..10766
                     /locus_tag="PKV59_00345"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HQP45227.1"
                     /translation="MKKNVIVSQSGGPSPVINSTLRGIVEMCRDIPDKFGEVYAGYHG
                     IEGVLKEELINLSAQNEEEIEYLSVTPAAGGIGTCRYKLRSSQKEDFDRVIDVFRAHN
                     IGYFFYIGGNDSMDTANKISLLANERGLDLVVVGGPKTIDNDVGDSEFKLIDHTPGYG
                     SVARYWALNIQNANEENEGSSPADPILVLQAMGRKIGFIPAAARLADPKREMPLQIYM
                     TESKVTLPELADNVNEELKRSKRCIVVISEGFDVGDIGARRDNFGHISFGSSQITVTQ
                     LVVNYLNEHGLKSKGLARGDMPGTDQRRNMIYASTVDMEEAYKLGQQATLIAASGETG
                     YMSTILRNPGPIYNVRYDKVPLDLVANSERFFPEAWIAPNRMDVTDDFVRYARPLIGD
                     NWPAIPLVNGIQRFARIEQIFVPQVLPEYVPEASR"
     gene            10866..11501
                     /locus_tag="PKV59_00350"
     CDS             10866..11501
                     /locus_tag="PKV59_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HQP45228.1"
                     /translation="MSDLLNEIKEAIIDGSAKIVSEKVTAALEEGFDPEQILNDAMIA
                     GMAEVGQLFEDGEYFVPEMLVAARAMTTGMQILKPRLVQADVKAAGKVAIGTVQGDLH
                     DIGKNLVGMMLEGASFEIIDLGADVPPEKFVEAVQKDHVQIVAMSALLTTTMPSMKTT
                     IEALEAAGVRDQVKVMVGGAPLSDQYAREIHADGYAPDANKAVALAKSLVS"
     gene            complement(11579..11785)
                     /locus_tag="PKV59_00355"
     CDS             complement(11579..11785)
                     /locus_tag="PKV59_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45229.1"
                     /translation="MRKSLSIVLLVVLLASIIAGCASSTATPKPTEVPSEAAPSEAAP
                     AEASPAVEATPTTQASISYSWFMM"
BASE COUNT         3586 a         2154 c         2775 g         3295 t
ORIGIN      
        1 atttttattt gaatccaaag aagagagaag aaaatgacgt gatgatgcgc cggatattat
       61 ttgaaaagtt cggagaattt ggtttcggtg aggaaaatcc caaaccgcgg cccattgccg
      121 gatcgatgat ggttgccggt gggtttgtta ttccagccct tctcggtgcc gaaatccgtt
      181 tttcgacggc ggaagccccg caacctttgc cccaaattcg atcaaaggat caaataatgt
      241 ctttcgtgga acccgatata ttatccaatt ggccgatgaa tgaattagcg gacggatggg
      301 atcggcaaca aaaagaatac ggttatttat tgggagatat taataccgac ggcattttga
      361 atgctgctta tcatttttac ggccccgatc tgtttgccga tttgcttgaa gatccggatg
      421 ttcctgttcg attcatgaga aaaatcgcaa aaataattat tcagaccgca acctatataa
      481 agaatcgtac cggtactact tccgtttccg taaatcggat ggcatgtcgt ttatctcctt
      541 cgccttttat tcatgccaac tgctccgtcc agatgatttc tccgcgaact tatgagaaga
      601 atctgttacc gattgaacag gacatggcca aattcatttc cgattacggc gttcatcatt
      661 gcggagtgaa aacagaaatc tatgcgaaat tttataacca aattcctgtt tgctactgcg
      721 aaatcggatg ggaatcaaat ccggagaaat gtcgatcatt tttgccgaat gcatttctga
      781 atctccgcct gagtccaatt cggatgcttt cttgttcttc ttctgaaatt aaaaacgatg
      841 tggaagaatt attaaattct gtaggcgatc tgaccaatgt gggagttagt tgtatcaata
      901 tggatttcgg tactcccgag gaaaatattt tgacggtatc ggagacaatc gaacgatata
      961 gaaccttatg gaaagaacat catggataaa ttaattcaaa atttttttca agcatcttgt
     1021 catcaagaag tttcaaaagt tccgttggca ttaattgctg acagtccgtg gattcccggt
     1081 tttgcgggaa ttgatacgct ggattattac cttgatcaga aaaaatggtt cgagatcaat
     1141 ttaaatcttc gagagcgttt tcccgaagtt gtctgggttc ccgggttttg gtgggaatat
     1201 ggcatggcaa tcgaggcttc tgcattcgga agtcgaatta tttttcatca tgatcaaccg
     1261 ccgtccatgg aacctatcag cacggattta cagttttgga aaaataaaac aaaagtcaga
     1321 gatcctgaaa aggatggctt gatgcctttg gccttgcgcc aaatcgagcg ttcggatcat
     1381 gaattacagt cctatggatt gggtcagcat attgccagtt cgcgcggtat cttaacggtt
     1441 acttcctggt ttatgggagt aacaacctta atggaatctc tcttgtccgc accggatgat
     1501 atcattccga ttctcgaagt tgtgacggat acgattattc tgtggttgga agctcaatta
     1561 aaacggatga gggagcccaa aggaattctt ttgctcgatg atttgatcgg aatgatttcc
     1621 aaaagagttt atttacgaat agtggatcct ctgttccgaa aaatatggga tcattttgac
     1681 ggttatgtga aaatctatca taatgacacc tcatgcgaac atttgtatcc tgtcctcgcc
     1741 gaaaccggat tcgatgtgtt caatttttcc gacaaaaccg atatcggaac agcccgtcaa
     1801 ttgatgggca aacaaatcgt cctgatggga aatgttgcgc cacgggatat cggagctctc
     1861 ggtactccgg aacaggttta ccaagccgcc tggaactgct tggaaaaaac aaacggagag
     1921 ggtctgatac tgtcttttgg cggcggaatt tctcccggaa ctcccgccga gaatattgat
     1981 tcgatgataa aagcgttgaa tgattggtat aaaagataat tttccgaaaa tcaatttttt
     2041 ggaattatta gagaaaggtt atcagatatg agtaaaaaat ggaatttacc ggaagatcgt
     2101 ttttttgata acgaacctgc tcaaaagaag atcgcactgg aaatttatga aagaatcaag
     2161 aatttgccga ttgtcagtcc tcacgggcat gtagatccta aattattcgc aaatgattca
     2221 cccggttttg gaactcccag cgacttattg gtcatcccgg accattatat tttcagactg
     2281 ttctactcac aaggactgaa attggaagat tttgggattc cttatcggaa tggtttgcaa
     2341 gatgcctccg aaaaagatca tcgaaaaatt tggcaacatt ttgccgataa tttttacttg
     2401 cttcgtacga cagcttctta tacatggctt gcctatgagt ttaagttcat cttcgatatt
     2461 gatgagattc cggattccga aaatgcgatg agaatctatg ataatctgac ggaaaagctc
     2521 agttcaccgg aatttcaacc gagagaacta tataagaaaa tgaacattga agtgctatgt
     2581 acaacagatg cggcttcaga ttcattgaaa acacatcaag aaataaaaga atccggttgg
     2641 gatggaaaga ttatcccgac ttttcgaccg gatgcggtta ccaatatcag aggagaaaac
     2701 ttccgacagg aaatcgaaaa attgtccgca gccgctgata tcgatatcca tgattttgaa
     2761 tcctatattc gggcgcttga aaaaaggcga gcctttttta aggaaatggg cgctgtatcg
     2821 acggatcagg ctgttattac cccttatact catgaacttc cgcaatccga aacggatagg
     2881 atttttaaag ctgctttaaa tggaaatgtt tccgattcgg atgctacggc atttgaaggc
     2941 catatgttga ttgaaatggc tcgcatgagc tgcaacgatc aattggttat gcaattacat
     3001 ccgggagtat taagaaacca taaccgaatc atttacgata aattcggtcc ggataaaggt
     3061 tgcgatattc cgattcaaac cgaatttaca aagaatcttc attcgatgct gaatcaatac
     3121 ggaaataatc ccaacttcac attatgttta ttcactgtcg atgagaatac ttattcgcgg
     3181 gaattagctc cgttagcggg tcattatccg tccgtccgtc tcggaccgcc atggtggttt
     3241 aatgacagtt ggaatggaat gacgcgattt tttgattccg taaccgaaac agccggatta
     3301 tacaatttga gcggttttgt agacgatacc cgagcgtttc cgagcatccc cgcaaggcac
     3361 gatgtttggc gtcgggtcgt ttctaattgg ctggcaaaaa tggtagtcag atcgttcatt
     3421 gatgtggatg atgcctacga tttcgcgttc cttttggctt attctcaacc taaaaatgtt
     3481 tataagcttt aatgattatt ttaaggatga aatagaatga aatatagtct gataaatact
     3541 tctgtaacaa caatcgattc gcaaaaaatt tggatgatca gagatgccga aggaaatcga
     3601 tatctgcaat ctgatcgacc gataaaggca ttgaaaagtg aaaataatga caatatttat
     3661 ttattgtcgc ctgaaaatgc aaagtggttg cgcagccaaa ttccttggtt aaacccccaa
     3721 cctttgggat taaaaaccag cgtcgggaca ggcgatcgct tagggatttc tacccctggt
     3781 catattcgag cttttcaagg gacccaaata tcccctgttt tggctcaaca atctgttcga
     3841 gaattgattc gaactcatcg aaccgctcaa atggtgcttg atgatgcaat gtggggtgtt
     3901 tttgaagcgg gatggaaccg tccttggggt gccgatgccg atcacttaaa gacgatcgat
     3961 gatatgagag aatactacga agcaggattt acattcttta caatcgatcc gggagaatat
     4021 gttaaaaacc ttaattcact ggataatatt cctttgcaat cattattaaa cagtattgat
     4081 tatgatcttt tagctacttc cgaatcagaa ttgattaata actatacgca ttttcccgat
     4141 tcgttagact ttataacggc ttccgaacaa gatatcaaat gcgcttttat aaaatatggc
     4201 ggagcaatcc atcatgttca aaaaatgtat tttcaattaa aaagttggat gggcaataaa
     4261 ccgtttgact tcgaggtatc ggtcgatgag acggatgaac cgacaacgat ttttgaacat
     4321 tatttcatcg cttgtgaatt aaaaaggttg ggcatcgaat ggacctcgct ggcacctcgt
     4381 tttgtgggaa aatttgaaaa aggtgtagat tatcaggggg atttgcatga attcgaatct
     4441 gaatatcgaa agcatgccgc aattcaaaaa tatttcggaa attataaaat cagtatccat
     4501 tcgggaagcg acaaattcag catctataaa atttgcgctg ttaacagtga atataaaact
     4561 cacttgaaaa ccgccggaac gagttatctt gaagccttac ggattatcgc aaccgttgat
     4621 ccggaattat tcagaaaaat ctatgatctc agtatgaatc gttatgaaat cgataaagct
     4681 tcctattatg tatcagcgca tcctgaaaat ttaccgaaaa tatcgagttt agcggatgat
     4741 cgactaccgg atttgttgaa tcaatttgat tctcggcaag tacttcatgt ctgctttggg
     4801 gcggtccttg atcaatacgg ctcggatatc cttcatgatt taaacagaaa ttatgatctg
     4861 tatcttgatg atttggaaaa acatttacga aagcatttgc aggattttat ttattccgaa
     4921 cctgcggaag aaagttgagg aataaatgac attttcaaac gaagttttcg gaaaaggaag
     4981 tcctgatccg aaagattttt taacggaatc ggatattaaa aagattttga cagacttttt
     5041 ttcggcaaat aatttaagcg gcaaaaaagt attggtcatt attccggaca gtacacgtac
     5101 gatgccgctt ccgctgtttt ttaaactgtt ttacgagctc atacatgatg aagttaaaac
     5161 gcttgatttt atgattgcgc ttggaactca tcctccgatg agcgatgagg caatcgcaga
     5221 tgaaatcgga ataagcatcg aagaaatgaa ggggaaatat caggatattt cttttttcaa
     5281 tcatcattgg gatgacccta aaacttttat tgaattaggt gaatttccta agaatgaggt
     5341 aattgaactg acaaagggaa ccgttcaacg ggcaattccg atccaaatca ataagatcat
     5401 ccttaattat gatgcactga ttgttttagg tcccgttttt cctcatgaag taatgggatt
     5461 ttcagggggg agtaaatatt tcttcccggg tatcagcggt ccggatctga ccaatacaac
     5521 tcattggatc ggagcgttgc atacttgtat aaaaacaatc gggctaaaga ttacaccgca
     5581 acgccaattg attgagcggg ctgcaaattt gattcccgtt ccgagacttt actgttgtcc
     5641 ggtcgtaaaa aaagagggca tttttggttt ttacgccggg gagccttatc aaacatggga
     5701 aaaagcggtc gatttatctt cacaggtgca tgtaaaatat gtcgataaac ctttcaaaaa
     5761 agttatttcg gttatgcccc atttatatga tgatatttgg acggcgggaa aagggatgta
     5821 caaactggaa cccgttgttg aagatggcgg tgaactgatt atttacgctc cgcatattac
     5881 tgaaatttct tatactcacg gtaaaacatt agaccggatc ggctaccact gtttggatta
     5941 ttttttgaag caatgggaga aattcaaaga tgagccatgg ggaattttag cccattcaac
     6001 ccatgttacg ggattgggaa cttatgagaa cggtatagaa aagaaacgga tcaaagtttc
     6061 tttagctacc ggcattccaa aggaaaggtg tgagcgagtg gatttaggtt atgtgaatcc
     6121 gcaagaatta aatctgcagg aatatgagca taaggaacat gaaggaatat tgcttgtccc
     6181 ttatgccggc gagactttat atcggttaaa acaatgaaca tactttcaaa gaagatacta
     6241 agaatgaaat gaaaaaaata aggagatgga tatgttaccg gatttcagtg taaaaggaaa
     6301 agttgttgtg attacaggcg gagggggagc catttgcgga atgatgagca aaatgttcgg
     6361 tgaagccggc gcgaaagttg cggtgatgga catttctatg gatgccgcaa agaaaattac
     6421 cgatgagata aataacaaag gcggagagtc gataccgtta gtgtgcaatg ttctcgataa
     6481 aaaaagtatc gaagatgccg ccgctcaagt catggggaaa tggggtagag ttgatatttt
     6541 gattaacggt gccggcggaa ataagccgca agctaccacc aataacgaaa tgtccttttt
     6601 tgacctgccg gcagatgcga ttcaatgggt attcaatcta aactgccttg ggacaattat
     6661 agccagccaa gtcttcggga aaataatggc cgagcaaaaa gaaggatgca ttttaaatat
     6721 atccagtatg aatgcttttc gtcctctgac cagaattccc gcttacagcg ctgccaaagc
     6781 agctattaac aactttactc aatggttagc agtcgatttg gcaaagaatt tttcgccgaa
     6841 tatcagagtt aacgccatag cacccggatt ttttgtgggg aatcaaaaca gagatctctt
     6901 aattgataag aattccggag atcttaccca gcgcgggaaa acaattatca gtctgacacc
     6961 gatgaatcgt tttggtgaat tcgaagatct tttaggcgcc actctcttct tagtttctcc
     7021 cgcctcttca tttataacag gcgtggtatt accgattgac ggcggttttt cttctttcag
     7081 cggtgtttaa tgggttcgaa aataaaggaa ctatgaacat gagcgaatta tcaaaccagg
     7141 cgaaagagtt gcttaatttc aaaccaaaac atgaattttt cgtcggaatt gattccgacg
     7201 gctgtgtttt tgattcaatg gaaatcaagc ataaagaatg tttttgcccg aatacaattc
     7261 gtgtttgggg tttacagccg atttcaaaat atgcccgtga agcggtggaa tttgtaaatc
     7321 tttattcaaa atggcgcggt atcaatcgat ggcctgcatt aattaaagtt tttgaattgt
     7381 tgtctgaacg tccggaagtg attcgtcggg gagccgttat accgaaagga gatgatatcc
     7441 gtgctttcat taatagcggc ttgccattaa gtgacgccgg gttgaaaaaa tatatggaga
     7501 ctcacagcag cgaagaactt gatcgagcgc tggaatggac gacatcggtt aatcgaacag
     7561 taaaagaaat agtccgcggc ttgaaacctt ttccgtatgc aaaagaaagt ctgcaagcca
     7621 ttcatccttt tacggatgct ttttgtgttt ccgcgactcc cgcagatgcc ttggaaaggg
     7681 aatggaaaga cggcgaaatt cgtgattatg tggaaatgat ttgcggtcag gaaatcggaa
     7741 caaaggaata tatcttaaga ctcggttctg ccggaaaata cccatcggat aaaaaattga
     7801 tgatcggtga cgctcccgga gatcttgaag cggcgcaagc ggtaggtgct ctcttctatc
     7861 cgattaatcc ggggcatgaa gaagaatcat gggaaagatt ctataaagaa ggatataagc
     7921 gtttcctcga aggaacttac agcggagaat atgaaaaacg cttgattgaa gattttgaga
     7981 agctgctgcc ggaaatcccg ccttggaagc gatgataaaa gattaaacaa atcccgaaat
     8041 aaataacgga atgataggta ggcaaataat ggaaaaagca aaaatcggac tgatcggatt
     8101 aggtgtgatg ggagccaatt tgtccttaaa cattgaacga aatggattcc ctgttgctgt
     8161 ttacaaccgg actgtcgaag aaacacataa ttttattaaa ggagcggctg ccggtaaaaa
     8221 gatttatggc gtagagactc tgaaagattt ggtggatatt ctcgaacgtc cgagaaaaat
     8281 tattcttatg gtgaaggccg gaagccccgt agatgaaacg attgaagcta taaaacccta
     8341 tcttgaaaaa ggagatatct tgatcgacgc ggggaactca ttcttccaag ataccgaacg
     8401 tcggagtgaa atgcttgcaa aagacgggat caattttttc gggatgggag tcagcggcgg
     8461 agaagaaggt gctttatggg gtccgagtat tatgcccggc ggtcagagag agtgttggga
     8521 agaattgcga ccggttctca cggcaatagc agccaaatat aaagacgggg aaccttgtgt
     8581 ggcttacatc ggtcccagag gcagcgggca ttatgtaaaa atggttcaca acggcattga
     8641 atatggggat atgcaattaa tagctgaaac ttatgacctg cttcaccgcg gcttaggttt
     8701 gggccaacag agattacagg aaatattcag taattggaat tccggagttt tgaaatccta
     8761 tttgattgaa atcacagcaa aaatattatc aaaaatcgat tctgagacgg gtaaaccgct
     8821 ggtagatgtt atcctggatg aagcgcaaca aaaggggacc gggaaatgga cagctcagaa
     8881 tgctttggat gtcggtgcac cgacgccgac attgacaagc ggtgtcgaag gaagaatttt
     8941 atcaagtttg aaagaggaac gtgttgttgc gagtaaaatt ctgaaaggtc cggatttaac
     9001 tttttacgga gatcgggaag aaataattcg agctgcggaa aatgcattgt attgttcgaa
     9061 aatcgcttcc tatgcacagg gattgggatt actgaaaatc gccggaaaag aatatgacta
     9121 taatctgaat ctgggtgaaa ttgctaaaat ttggcgagaa ggatgcatta ttcgcgcgga
     9181 tttcttaaat gatataaccg acgcttttaa taaaaatccg tctcttcaaa atctgatgtt
     9241 ggatgatttt ttccgatctg ccattgaaac aagacaatca gattggagat taattgcaaa
     9301 aacggctatt gatctcggaa ttccgatgct tgcgacgaat gcatcattgg catattatga
     9361 cagctatcga agtgaaacgc ttccggcgaa tttaacgcag gcacagcgag atttctttgg
     9421 tgcgcatacc tatcgcagaa ttgataaaga aggaagtttc cataccatat gggaggaata
     9481 aagtgaaaaa gaatgtgatt gtttcacagt ccggcggtcc gtctccggtc attaatagca
     9541 ctctgcgcgg tattgtggaa atgtgcagag atattccgga taaattcggt gaagtttatg
     9601 cggggtacca tgggatcgag ggtgtcctga aggaagaatt aattaattta agcgcacaaa
     9661 acgaggagga aattgaatat ttgagcgtga cgcctgccgc cggtggaatt gggacatgcc
     9721 ggtataaact cagatcctcg caaaaggaag attttgatcg ggttattgat gttttcagag
     9781 cacacaacat cggatatttt ttctatatcg gcggaaatga ttctatggat acggcaaaca
     9841 agatatctct tttggcaaat gaaagagggc tggatttggt tgtcgtcggc ggtccgaaaa
     9901 caattgataa tgatgtgggc gacagtgaat ttaaattaat cgatcatact ccgggatatg
     9961 gaagtgtcgc aagatattgg gcgttgaata ttcaaaatgc caatgaagag aacgaaggtt
    10021 ccagtccggc tgacccgatt ttggttttac aggcaatggg gaggaaaata ggatttattc
    10081 cggctgcagc cagattggcg gacccgaaga gagaaatgcc tctgcagatt tatatgacgg
    10141 aatcaaaagt aactcttccc gaattagccg ataacgttaa tgaggaactg aaaagaagca
    10201 aaagatgtat tgtggtcatt tcggaaggat ttgatgtcgg agatattggc gccagacgcg
    10261 ataatttcgg acacatttcc ttcgggtcga gccaaattac cgttacgcaa ttggtagtga
    10321 attatctgaa cgaacatggc ctgaaatcaa aaggcctggc tcgaggagat atgcccggaa
    10381 cagatcagcg tcgcaatatg atttatgcat ctaccgtgga tatggaagag gcttataaat
    10441 tgggtcaaca agcaactttg attgccgcat cgggagaaac gggatatatg agtacgattt
    10501 tacgaaatcc gggcccgatt tataacgttc gatatgataa ggtcccgttg gatttagtcg
    10561 ccaattccga acgttttttc cccgaagctt ggattgctcc aaaccgaatg gacgtgacgg
    10621 atgattttgt ccgctatgcc cgcccattga ttggagataa ttggcctgcc attccactgg
    10681 tgaacggaat tcaacgtttt gcaagaattg aacagatttt cgtcccacag gtacttccgg
    10741 aatatgttcc ggaagcatcg aggtaatcga gttactgaaa tccggatttt gaaatcgaaa
    10801 acgagatcaa acgataatca tgataatatt gcagataatc agattaattg ataaagagga
    10861 attatatgtc agatttgtta aatgaaatca aagaagcaat tattgacggg tcggcaaaga
    10921 ttgtttccga aaaagttacc gccgcactgg aagaaggttt cgatccggaa cagattttaa
    10981 atgatgcaat gatcgccggt atggcggaag tgggacaact ttttgaggat ggagaatatt
    11041 ttgtgccgga aatgttggtc gctgcacgtg caatgactac cggaatgcag atactgaagc
    11101 cccgccttgt tcaggctgat gtaaaagccg ctggaaaagt tgcgatcggt acagtccaag
    11161 gcgatttaca tgatatcgga aagaatttag tcgggatgat gcttgaggga gcctcttttg
    11221 aaattatcga tttaggcgct gacgtgcctc cggaaaaatt tgtggaagcc gttcaaaaag
    11281 atcatgttca aattgtcgcc atgtcggctt tactgacgac gacgatgcct tcgatgaaaa
    11341 cgacaatcga agcgcttgaa gcggctggtg ttcgtgatca ggttaaagta atggtcggcg
    11401 gagctcctct ttcggatcaa tacgcaagag aaatccatgc ggacggatat gcaccggatg
    11461 cgaacaaggc tgtggcgtta gcaaaaagtc tggtttctta aaatgaagag ataataaaaa
    11521 gaaaaggtgc agaaaaaatg caccttttct ttttattatc agtcagttaa ctggtcaatt
    11581 acatcatgaa ccaagagtaa ctaattgaag cctgggttgt cggagttgct tcgacagcgg
    11641 gtgaagcttc ggcaggagca gcttctgaag gagctgcttc gctaggaacc tcggtcggtt
    11701 tcggtgtcgc agtagatgat gcacagccgg caatgatact ggctaacaaa acaactaata
    11761 aaacgatact taaagatttt ctcatttaat tcctccacga tttagtatat
//
LOCUS       DAOMIV010000007        11432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_5653_length_11432_cov_4.701064, whole genome shotgun sequence.
ACCESSION   DAOMIV010000007 DAOMIV010000000
VERSION     DAOMIV010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 11432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11432
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_11432_cov_4.701064"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="PKV59_00360"
     CDS             complement(<1..1303)
                     /locus_tag="PKV59_00360"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQP45230.1"
                     /translation="MEKQYKLSGLGCPVCASNIEQKINDIPGVRNATVNFATQTLEIE
                     TESENEIPLFSELEEIVHTYEPETTLKEYSPAENSFEKEERKPKKFRKRIITFVLSAI
                     LAVAGSMQPEASAMRIFLFLLAYLIAGIRVIRKAINSIRHGNWFDENFLMSIASIGAV
                     AIGEFPEAVAVMIFYEVGELLQESAVDHSRKSIASLMNIRPDYANLVTGDGTLRVNPA
                     DVKIGDLIQVKAGERVPLDGIVEEGHSMLDTSALTGESVPREVFPGDPSLSGSINQSG
                     VLTIRVNRTFGESTVNRILDMVENASEKKAATEKFITKFSKIYTPIVIGLAFLIAVIP
                     PLIGGGSWSPWLYRGLVFLVISCPCALVISIPLGFIGGIGKASKKGILVKGSDYLEAL
                     NNVSIAAFDKTGTLTKGTYNVTKVLPAPGFSESYLLETAAYA"
     gene            complement(1316..1675)
                     /locus_tag="PKV59_00365"
     CDS             complement(1316..1675)
                     /locus_tag="PKV59_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQP45231.1"
                     /translation="MTEAENFSDCTVLHEDTVKAVQAQMPDEPQFYDLADLYKVFGDT
                     TRIKILWALDIHEMCVCDLAVLLNVTKSAISHQLRILRQENLVKPRREGKEVFYSLAD
                     DHVRSIFEKGLEHINEL"
     gene            1893..2249
                     /locus_tag="PKV59_00370"
     CDS             1893..2249
                     /locus_tag="PKV59_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQP45232.1"
                     /translation="MSTEQKINGNIYISHRAISTIAYHAALESYGLVGLAPKNKAAGL
                     ANVLIKDPTLGVSIEFDGSGVGIDLYIVVEYGTRITSVASSVADNVSYQVENQTGLTV
                     KYVNVHVRRLRVSCPD"
     gene            2284..3960
                     /locus_tag="PKV59_00375"
     CDS             2284..3960
                     /locus_tag="PKV59_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HQP45233.1"
                     /translation="MEFIPNNPQTDQKTNVLTVLNAQQVKSLIRAGLLWLKTNQEIVN
                     SLNVFPVPDGDTGSNMVLTMQAGLNEIVDSPADSVDVLINQVARGALMGARGNSGVIL
                     SQFWRGFSRAIDHQPVLTMELFAGALTEAKRTAYKGVVRPVEGTMLTVLKDTSEKVNQ
                     CLPDCKDFLQLFQITVAAADESVNRTPELLPVLKQAGVVDSGGKGLFFIFEGFLRSLE
                     NKPLDVNPEVIEIVPIDLMKEEESIEPGQDYEVVVDFVPNTQLELSTFYDRLQDIGTS
                     VQVGEGDNNYRMHIHVPAENLYEPINYILTLGTVTKVAIENLVAQMEDLRRQQQCKYD
                     LTPIRDGQIGVVAVSPGDGISRIFASLGVGKIIRGGQTMNPSTEEILNAIESLPTDKV
                     IVLPNNKNVILAANNAITQTNKKVAVIRSKNVPQGISAMLRLDPDGDFDTIVSEMNES
                     LTDIQTGEVTRATRTVEINDVPVNEGQYIVMHNGDLAIAKDGLEEAVFAFLERINVTD
                     YERITIFYGQDMKEADVNQLVAKIKERYPDIEVECHYGGQPYYFYILSIE"
     gene            4219..>5452
                     /locus_tag="PKV59_00380"
     CDS             4219..>5452
                     /locus_tag="PKV59_00380"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45234.1"
                     /translation="MEKFLPIWRREAPTEGVSEEIIEKVSKFLSEYSDFDKERREQSI
                     RNLLNDLGSPELFDRYKQRYSRAETTNRQNHYSGGENRSKEESSAPDYPEGSKYDHES
                     SNSLRSESSVKPERKPVRIHENHNEASTIDSPVKIIPGIGPQNAKALARQGVYTIRDL
                     LYYFPRRYDDYSKFKTINKLLPGEKVTIVCVVYSVENVVRGRYQITEAVVGDGTGMIR
                     VSWFNQPWLIHQIQPGSSYVFSGKIDVFLGRPVMNGPEFEPTEQENLNTNRIVPVYPV
                     NSNLKQNYLRRMIFNTVRHWANLIPEFLPETVLESADLLPLSKAVEEIHFPDSAELLE
                     AARKRLAFDELFLVQLDVLRRKRNRTSATANRFQISDERMDRWIKSLPYELTTAQRRV
                     LNEIRGDIDSGKPMNRLIQ"
     assembly_gap    5453..5552
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5553..6839
                     /locus_tag="PKV59_00385"
     CDS             <5553..6839
                     /locus_tag="PKV59_00385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQP45235.1"
                     /translation="NEIRGDIDSGKPMNRLIQGDVGSGKTIVACLAALIISDAGGQSV
                     LMAPTGILAEQHFRSFRDIIEKIRENSGDLNIDPNRIALLTGSTPESEKKEIYEKSAS
                     GEIAIVIGTHALLEDPVQFKNLDFIVVDEQHRFGVEQRATLRSKGRNPHLAVMTATPI
                     PRSLALTVYGDLDLSVIDEMPQGRLPVKTFVVNPLGRERVYRHVIKEVKNGHQAFIIY
                     PLVESGDDEQKEGKSAVEASEFLDKEVFPEQRVALLHGRMKGEEKDAILEAFKNKEYD
                     ILVSTSVIEVGVDIPNATTIVIEGANRFGLAQLHQFRGRVGRGNEQSYCFLIAENDDA
                     IENERLLAMTQTNDGFKLAELDLNQRGPGDFLGSRQSGFMDFKLASFTDLKLIESARN
                     EALKVFEADPELSSEENKLLKEQLEKLNTIHIGELS"
     gene            6839..7339
                     /gene="coaD"
                     /locus_tag="PKV59_00390"
     CDS             6839..7339
                     /gene="coaD"
                     /locus_tag="PKV59_00390"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HQP45236.1"
                     /translation="MKIRAIFPGSFDPVHYGHIDIAARASRIFDSLVVAVYETPSKNL
                     FFSTEERLEMLRQAMANLPNVEVVSYRGLTIGFAKQINAQVMVRGLRVFSDFDNEFRM
                     ALTNQELDPTIETISLITAHQHTFVSSTTVKEIAELDGDVSSMVPDFVAKRLKEKFAS
                     KKDEII"
     gene            7470..7943
                     /locus_tag="PKV59_00395"
     CDS             7470..7943
                     /locus_tag="PKV59_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45237.1"
                     /translation="MDILNLVDKLEELFSQGRSIPMTHNVIVDEDRMLDIIDQMRISI
                     PDEIKRSQQILAQRDRILAQAKEEHDRTIEIAKEKREQLTSSSEITAEAKKRADQIIS
                     QAYTEAEVIKREADKYALDTLKRLELEMDRTINQVRNGIRTLQRDQESDSNRPES"
     gene            complement(7949..10894)
                     /locus_tag="PKV59_00400"
     CDS             complement(7949..10894)
                     /locus_tag="PKV59_00400"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQP45238.1"
                     /translation="MKKDLTVLLKDWYGYHWHKTQQSLVEAMELCESQEAKTKQIQQE
                     YAVIEQDIAAVQEEIRGKRILLGELHDQLSKYHHAMDESSRESSILDERLNAARRQIA
                     AYREESELIEAEITLLIERREKVIAESAELKKAGAIAFEQKTQLEAELAAKQKELSAL
                     QNKRNESRSLLTKTETTLIQLKARRDETQNRLDALAESYSALQTALVESETILAELQK
                     KQTEIQNDLKAIESDSLKSEQEQNEIRNKLRDLEEKKAGLSDKLNQNQAQLTRYKTQY
                     EVLNQAEKNLTGLNQGAQFLLKNIGKVIDEKEFRSLGSCFIFPKEVEIAAAAVLGEYL
                     DAILLNRDKWEKAADLLSSGNNGRAVFISEKDNAKDTPPVSDSKIAAQKSLADLAICD
                     PEYKTLAERLFRKAYLADSFKQASELQPLLQAGESLVTPEGDLFRANGSMATGKEPKY
                     QLISRKREMKELRELIEDRTEVIQTVRDELNEINQNIQTVQKQAELTAERHRNQSREL
                     EALRKSFNQAVIETQKREQLIAFQKQRIQENEQQSLTLKAKKESDDAKISELEAELSG
                     LRETVKKLSDAVHQIQLDDLQQQVHHRTLEWTVNEKSIHEAETQIAELTAQIDKNQQR
                     SAQNLEKKTALEQQILTVQEQIESLKSNNTLSGMNAAEIEAKIAPEENALKNLQQRLE
                     TLQSELQIKQTKTSAAEKQLLQQQMEVTRLNDQLDTLKQRIEDDFGLVAFDYKEKVAG
                     QTPLPLGDMVSELPKVEELPENIKDQISRQKGLINRIGAINPDAIEEYESVNERYNFL
                     LTQSNDLKSADRDLREVIGELDEMMKNAFQETFEKVQAEFKNMFTRLFGGGEARLVLT
                     DPDNINQSGIEIEAKLPGRREQELSLLSGGERSLTSVALVFALLRVSPTPFCVLDEVD
                     AALDESNVGRFCELLKDLSRETQFIVITHNRNTVETADVIYGITMGKESSSQVVSLRL
                     DEINKDYLI"
     gene            complement(10891..>11432)
                     /locus_tag="PKV59_00405"
     CDS             complement(10891..>11432)
                     /locus_tag="PKV59_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45239.1"
                     /translation="NIADAIRWVLGEQSYTVLRGRKTMDMIFTGSDQKARSSMASVSI
                     LFDNQEEWLPIDYEEVEITRRAYRSGENEYQVNGQRVRLKDINELLGQSGLSERTYTI
                     IGQGLIDSALSLKPDDRRRFFEEAAGIGLFRSRREEAINRLEITQHNLERINDILGEL
                     EPRLKSLQGQSKRAAEYEQ"
BASE COUNT         3025 a         2527 c         2612 g         3168 t
ORIGIN      
        1 ccgcatacgc tgcggtttcc aacagataac tttcggagaa tccgggcgca ggcagcactt
       61 tggtgacgtt gtaggttcct tttgtcaatg tcccggtctt gtcgaaagct gcgatactga
      121 cgttattcaa cgcttccaaa taatcggatc ctttgaccag aattcctttt ttggaagctt
      181 tcccgatccc gccgatgaac cccaacggaa tggaaatgac cagtgcgcaa ggacatgata
      241 tgaccaaaaa aaccaatccg cgataaagcc atgggctcca agaaccgcca ccgatgagcg
      301 gtggaataac cgcgattaga aatgccagtc cgataacgat cggggtgtaa atctttgaaa
      361 atttggtgat aaatttttcg gtagccgctt ttttctcgct cgcattttcg accatatcca
      421 aaattcgatt gactgtcgat tcgccgaaag ttctgtttac ccgaatcgtc agtacacccg
      481 attgattaat cgagccggac aaagaaggat ctcccggaaa aacttcccgc ggtacggatt
      541 ctcccgtcag agcggacgta tccaacatcg aatgtccttc ttccactatg ccgtccagcg
      601 ggaccctttc gcccgctttc acctgaatta aatctccgat tttgacatcg gccggattta
      661 cccgaagcgt cccgtcgccc gtgactaaat tggcataatc ggggcggata ttcatcagac
      721 ttgcgattga ttttcgcgaa tgatcgaccg ccgattcctg caataactct ccgacctcat
      781 aaaaaatcat cacggcaacc gcttccggga actctccaat ggcaacggct ccaattgaag
      841 cgatactcat caaaaagttt tcgtcgaacc aatttccgtg ccggatactg ttgatcgcct
      901 ttcgaataac tctgatgccg gcgataagat aggccaaaag aaacagaaaa attcgcatcg
      961 cgctcgcttc gggttgcatg cttcccgcaa cggcgagaat ggccgataaa acgaacgtaa
     1021 ttatcctttt cctgaatttt ttcggttttc tttcttcttt ttcaaaagaa ttttcggccg
     1081 gggaatactc tttcagcgtt gtttccggtt cataagtatg gacaatttcc tctaattccg
     1141 aaaacagcgg aatttcattt tccgattcgg tttctatttc aagcgtttgt gtggcaaaat
     1201 tcacggtggc gtttcggacg ccgggaatat cgtttatttt ttgttcaata ttggatgcgc
     1261 aaacgggaca acctaaaccg gataatttgt attgtttttc catactttcc ttgttttaca
     1321 actcgttgat atgttccaat cctttttcaa aaatcgaacg gacatggtca tcggccaatg
     1381 aataaaaaac ctctttccct tcacgacgcg gcttgaccag attttcctga cgtaagatgc
     1441 gtaattgatg cgaaatagcc gatttcgtca cgttcaataa aaccgccaga tcacagacac
     1501 acatttcatg aatgtccaac gcccaaagaa ttttgatacg tgtcgtatct ccaaagactt
     1561 tgtataaatc ggccagatca taaaattgcg gttcatccgg catctgtgcc tgtacggctt
     1621 tcaccgtatc ttcatgcaat accgtgcaat cgctgaaatt ctccgcttcc gtcataattt
     1681 cctttctgaa aacaattgat caattgttca actgttattt tattccgatt ttgtcatttg
     1741 tcaagaaggg gattgacata actttcgata ttcatcccaa aacatttcga ttaacggtta
     1801 aaatgggaaa agctggaaac cttttttacg aaccggatcg gcattgagtt ttgatttgat
     1861 ccggaaatat gtgcggaaaa ggaagatgtt gaatgagtac tgaacagaaa attaatggaa
     1921 acatatatat ctctcatcgg gcgatatcga ccatcgcata tcatgcagct cttgagtctt
     1981 acggattggt tggtttagct ccgaagaata aagctgccgg acttgcgaat gttttgatta
     2041 aagatccgac tctcggagtg agtatcgagt ttgacggatc cggtgtcggg atcgatttgt
     2101 atatcgttgt cgaatacgga acaagaatca catcggtcgc atcgagtgtg gctgataatg
     2161 tgagctatca ggttgaaaat caaacgggtc taaccgttaa atatgtcaac gtccatgtac
     2221 gcagattacg cgtcagctgc ccggattaat ggggattcta aggaaagaaa tatcaggaga
     2281 attatggaat ttatacccaa taatccgcaa acggatcaaa aaacaaacgt attaaccgta
     2341 ttaaacgcgc agcaagtcaa atctttgatc agagccggtt tactttggct gaagacaaac
     2401 caagaaattg ttaactcatt gaacgttttt cctgttcccg acggagatac cggatccaat
     2461 atggtattaa cgatgcaagc cggtttgaat gaaatcgtcg attcaccagc cgacagtgtc
     2521 gatgtattga tcaatcaggt agctcgggga gcattgatgg gcgctcgcgg aaacagcggc
     2581 gtgattctgt cccaattttg gcgcggattt tcccgtgcga tcgaccatca accggtttta
     2641 acgatggaat tatttgccgg cgcgctgacg gaagccaaac ggacggctta taaaggtgtc
     2701 gttcgcccgg ttgaaggcac gatgctgacg gttttgaaag acacatccga aaaagtgaat
     2761 caatgtcttc cggattgtaa agatttcctg caactgtttc aaatcaccgt tgccgcagcg
     2821 gatgaatcgg taaatcgcac cccggaacta ttaccggttt tgaagcaagc cggtgtagtg
     2881 gactccggcg gcaaagggct attttttatc tttgaagggt ttttaagatc attggaaaat
     2941 aaaccgttgg atgtaaatcc ggaagttatt gaaattgtgc cgattgattt gatgaaagag
     3001 gaagaatcga tcgaacccgg gcaggattat gaagtggtgg ttgattttgt acccaatacc
     3061 caattggaac tttccacttt ctacgaccga ttacaagata tcggaacttc ggtgcaagtc
     3121 ggcgagggtg ataataatta tcggatgcac atccatgttc cggctgagaa tctttatgag
     3181 ccgatcaatt atattctgac attgggaacc gtaaccaaag tcgccatcga aaatctggtg
     3241 gctcaaatgg aagatttacg ccggcagcag caatgcaaat atgatttgac tcctatccgg
     3301 gacggtcaaa tcggtgtggt agcggtgtca cctggggacg gaatatcgcg tatttttgcc
     3361 agtttaggcg tcgggaagat catccgcggc ggacagacaa tgaaccccag taccgaagaa
     3421 attttgaacg cgatcgaatc cttgccgacc gataaggtga ttgtccttcc caataataag
     3481 aatgtgattt tggctgccaa taacgccatc actcagacga acaaaaaagt cgccgtcatt
     3541 cgcagcaaaa atgttccgca ggggatcagc gcgatgcttc ggttggatcc agatggggat
     3601 ttcgatacca ttgtctccga gatgaacgaa tccttgaccg atattcagac gggtgaagtg
     3661 accagggcga cacggacggt agagatcaat gatgtaccgg ttaatgaggg acagtatatc
     3721 gtcatgcata acggtgattt ggcgatcgca aaagacggtt tggaagaagc tgtttttgcc
     3781 tttttagaac ggataaacgt aacggattac gaacgcatca ccatctttta cggtcaggat
     3841 atgaaagaag ccgatgtaaa tcagctggtc gcaaaaataa aagagagata tccggacatt
     3901 gaggtggagt gccattacgg cggtcaaccc tattattttt atattctttc aatcgaatag
     3961 cactacaaaa cagagaaatc gaaaagggga ttcgaaatgc aaacgtcgat ttatcggaaa
     4021 aaatccgata aatcgatgca aaacatcgga atgatgctct ttttttattg aaaattccaa
     4081 aaaaatgacg gatcgagaaa tcggctccga aaaaaaatgg gttaaaatag ggacatgttg
     4141 tcttccacag aaaaaataaa gaaattcatc agtttggaaa ttgagcgagg ttatgataac
     4201 cgctctgttg tagggggaat ggaaaaattt ctcccgattt ggcgtcggga agccccgaca
     4261 gaaggtgtat ccgaagaaat tattgaaaaa gtcagtaaat ttttgtccga atacagtgat
     4321 ttcgataaag aacgccggga gcaatcgatt cgaaatttgt taaacgattt gggatcaccg
     4381 gaactgtttg accggtataa gcaacgttat tcaagagcag aaacaaccaa tcgacaaaat
     4441 cattattccg gcggggaaaa tcgctcaaaa gaggaatcat ccgcgcctga ttatccggaa
     4501 ggatcgaaat atgatcatga aagttcaaat tctctaagat cggaatcatc tgtcaagcct
     4561 gagagaaaac ccgttcggat tcatgagaat cataatgaag cctcaacgat tgacagcccg
     4621 gtgaaaatta tccccggaat cgggccgcag aatgcaaaag cacttgcccg gcaaggcgtc
     4681 tatacaatcc gcgatttact gtactatttc ccgcgtcgat atgatgatta ttcgaaattt
     4741 aaaaccatca acaaactcct tcccggggaa aaagtgacca tcgtctgcgt ggtttattcc
     4801 gtcgaaaatg ttgttcgcgg aagatatcag attacggaag ccgttgtcgg ggacggaacg
     4861 gggatgattc gggtcagctg gttcaaccag ccttggctaa ttcaccaaat tcaaccgggt
     4921 tcctcttatg tattctcggg aaaaatcgat gtgtttttgg gaagaccggt gatgaacggg
     4981 ccggaatttg agccgaccga gcaggaaaac ctgaatacga accgaattgt gccggtttat
     5041 ccggtgaatt ccaacctgaa gcagaattat cttcgccgga tgatatttaa taccgttcgc
     5101 cattgggcaa atctgattcc ggaattttta ccggaaacgg tattggaatc agccgatctg
     5161 ttgccgctct caaaagcagt cgaagaaatc cattttccgg attcggcaga attgctagag
     5221 gcggcacgaa aacgattggc ttttgatgaa cttttcctcg ttcagttgga tgtattacgg
     5281 cgaaagagaa accgaacttc ggctaccgca aaccgttttc agatttccga tgagcggatg
     5341 gaccgctgga taaagagttt gccttatgaa ctgacgaccg ctcaacgtag agtgctgaat
     5401 gaaattcgcg gagatatcga ttcgggcaaa ccgatgaatc gactgattca ggnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatgaaat tcgcggagat atcgattcgg
     5581 gcaaaccgat gaatcgactg attcagggag atgtcggatc cggtaaaaca attgtcgcct
     5641 gtttagccgc tttgattatt tccgatgcgg gcggacagtc tgttttgatg gcaccgaccg
     5701 gaatcttggc ggaacaacat ttccgttcct ttcgggacat catcgagaag attcgcgaaa
     5761 attccggtga tctgaatatc gatccgaacc gaattgccct attaaccggc tcgactccgg
     5821 aatcggaaaa gaaggaaatt tacgagaaat cagcttccgg agagatcgca atcgtcatcg
     5881 gaacgcatgc tttattggaa gatccggttc aattcaagaa cttggatttt atcgttgtcg
     5941 acgaacaaca tcgcttcggc gtcgaacagc gtgcgacact tcgttcgaaa ggcagaaacc
     6001 ctcatttggc ggtgatgacc gctacgccca ttccccgatc gctcgcattg acagtttacg
     6061 gggatctgga tttatccgtc atcgatgaaa tgccccaggg aagattaccg gtcaaaacgt
     6121 tcgtggtcaa tccgttggga cgggaacgtg tttatcggca tgtgattaaa gaagtcaaaa
     6181 acggacatca ggcttttatt atttacccgt tggtcgaaag cggtgacgac gaacagaagg
     6241 aaggaaaatc cgctgtggaa gccagcgaat ttctcgataa agaagtcttt ccggaacaac
     6301 gggtcgcgtt gttgcacggc cggatgaaag gcgaagaaaa agacgccatt ctcgaggcat
     6361 tcaaaaataa agaatacgat attttggttt cgacttcggt aatcgaagtc ggagtggata
     6421 ttcccaatgc gacgaccatt gtaatcgaag gggcaaatcg cttcggtttg gcacaattgc
     6481 atcaattccg cggtcgggtc ggtcggggaa atgagcaatc ctactgtttt ctgattgccg
     6541 aaaatgatga cgccatcgaa aacgaacgct tgctcgctat gacccaaacg aacgacgggt
     6601 ttaaattggc tgaattggac ttgaatcaac gcggtccggg tgactttttg ggaagtcgtc
     6661 aatcgggatt catggatttc aaattggcat cttttacgga tttgaaatta atcgaatcgg
     6721 ccaggaacga agctttgaaa gtctttgagg ccgatcctga actgtcttcg gaagaaaata
     6781 aattattaaa agaacaactc gaaaaactca acacgattca tataggagaa ctcagttaat
     6841 gaaaattcgg gcaattttcc cgggaagttt tgatccggtc cattatgggc atatcgatat
     6901 cgctgcgcgc gcttctcgga tttttgatag tctggtggtt gcggtttatg aaacccccag
     6961 taaaaatctg tttttttcta cagaggaacg cttggaaatg ctgcggcaag cgatggcgaa
     7021 tctgcccaac gttgaagtgg tcagttacag gggattgacg atcggctttg ccaaacaaat
     7081 caatgcgcaa gtgatggtgc gcggattacg cgttttttcg gattttgaca atgaatttcg
     7141 catggcgttg accaatcagg agctggatcc gaccatcgag acgatttctc ttattacggc
     7201 tcatcaacat acttttgtct cctcgacaac cgtgaaagaa atcgccgaac tggacgggga
     7261 tgtttcatca atggttccgg attttgtggc aaagcgtctg aaagaaaagt tcgcgtctaa
     7321 aaaagacgag atcatataat atcgaagatc gaaagaatcg tctgataaga ttattaaacg
     7381 aatctatgtt taaaatgttg taaagtgaat tcattcatgg cataataatt tattatgatc
     7441 aatcggtcaa aagaggaatc ggaggaagta tggacattct gaatcttgtt gacaagctgg
     7501 aagaattatt cagtcaggga cgctcgattc caatgaccca caatgtcatc gttgatgaag
     7561 atcgcatgct ggatataatc gatcaaatgc ggatttccat tccggatgaa attaaaagaa
     7621 gccaacaaat cttagcgcaa agggatcgaa ttttagccca ggcgaaagaa gagcatgatc
     7681 gaaccatcga aattgccaag gaaaaaagag aacaactgac ctccagcagc gaaattacgg
     7741 cggaagccaa gaaacgggca gatcaaatta tttcacaggc ttataccgaa gccgaagtga
     7801 tcaaacggga agccgacaaa tatgccctgg atactttgaa acgcttggag ttggaaatgg
     7861 atcggacgat taatcaggtt cggaacggga tccgtacgct ccaacgggat caggaatccg
     7921 acagcaaccg cccggaaagt taatccgttc agatcaaata atctttatta atctcatcca
     7981 accgcaagct gacgacttgg ctggaagatt cttttcccat cgtgatgccg taaataacat
     8041 cagctgtttc gacggtattt cgattatgtg tgataacaat aaattgtgtt tcacgactca
     8101 aatctttgag cagttcgcag aatctgccga cattggattc atccaacgcc gcgtcaactt
     8161 catcgaggac acagaacggg gtagggctga ctctcagcaa agcaaaaacc aacgcaaccg
     8221 acgtcaggct cctttctccg ccggaaagca acgaaagttc ctgctctcgt ctgcccggta
     8281 atttcgcttc gatttcgata ccggattggt tgatgttatc ggggtctgtc aaaaccaggc
     8341 gggcttctcc gccgccgaat aaacgcgtga acatattttt gaattccgcc tgcacttttt
     8401 cgaaagtctc ttgaaatgcg tttttcatca tctcatccaa ttccccgatg acttcccgca
     8461 aatcccgatc cgccgatttt aaatcgttgg attgagtcag taaaaaattg tatcgttcat
     8521 tgacggattc atattcctcg atcgcatcgg ggttaatcgc tccgatgcgg ttgattaatc
     8581 ccttttgtcg ggagatctga tccttgatat tctccggaag ctcttcgact ttcggcaact
     8641 ccgagaccat atcacccagc ggaagcggtg tttgtcccgc tactttttct ttataatcga
     8701 aagcaaccaa accgaagtca tcctcgatcc gttgttttaa agtatcgagt tgatcattta
     8761 aacgggtcac ttccatttgc tgctgcaata attgcttttc cgccgccgaa gttttggttt
     8821 gtttgatttg caattcggat tgaagcgtct ccaaacgttg ctgcaggttt ttaagcgcat
     8881 tttcttcggg ggctattttc gcttcgattt ccgctgcgtt cattccggaa agcgtattgt
     8941 tactttttaa agattcgatc tgttcctgca ctgtaaggat ttgctgttcc aatgccgtct
     9001 ttttttcaag attttgagcg gatcgttgtt gatttttatc gatttgagcg gtcaattcgg
     9061 caatttgcgt ctccgcttca tggatggatt tttcatttac ggtccattcc aacgtccgat
     9121 gatgaacctg ttgttgcaag tcatccagct gaatttgatg aacggcatcc gaaagctttt
     9181 tcaccgtctc gcgtaaaccg ctcaattcag cctcaagttc gctgattttc gcatcgtcgc
     9241 tttctttttt tgctttcaga gtgaggcttt gctgctcatt ttcctgaatt ctttgttttt
     9301 ggaatgcgat caattgttcc cgcttttgcg tttcgatgac ggcttgatta aaactttttc
     9361 gcagcgcttc cagttctcgg ctctgattcc gatgccgctc ggcggtaagt tcggcttgct
     9421 tttgcaccgt ttggatgttt tgatttattt cgttaagctc atctcgaaca gtctgaatga
     9481 cctccgtccg gtcttcgatc aactctcgga gttctttcat ttcccttttt ctggaaatca
     9541 gttgatattt cggctcttta ccggttgcca tgctcccgtt cgcccgaaac aagtcccctt
     9601 ccggagtcac caacgattcg ccggcttgta acaaaggttg taattccgac gcctgcttaa
     9661 aagaatccgc caaataagct ttacggaaaa gtcgttcagc cagcgtttta tactcggggt
     9721 cacagattgc caagtcggcg agcgatttct gtgcggcgat tttcgaatcg gaaacgggtg
     9781 gcgtatcttt tgcgttatct ttttcgctaa taaagacggc tctgccgtta tttccgcttg
     9841 aaagcaaatc cgcggctttt tcccacttgt ctcgattcag aagaattgcg tcaagatact
     9901 caccgaggac cgcagccgca gcgatctcga cttctttcgg gaagataaaa caactgccca
     9961 gcgatcgaaa ttctttctca tcgattactt tgccgatatt tttcagcaga aattgagcgc
    10021 cttggttaag tccggtcagg ttcttttcgg cttgatttaa aacttcatac tgagttttat
    10081 atcgcgtcaa ttgcgcctga ttttgattga gcttgtccga aagcccggct tttttttctt
    10141 cgagatctct cagcttgttt cgaatttcat tttgctcctg ttcggatttc aagctgtccg
    10201 attcgattgc cttcagatcg ttttggattt cggtttgttt tttttgcagt tccgccaaaa
    10261 tcgtctcgct ttcaaccaaa gcggtttgca gagcggaata ggattccgcc aaagcgtcca
    10321 aacgattttg tgtctcatcc cgccgtgctt tcaattgaat caacgttgtt tcggtttttg
    10381 tcaacaaaga acgactttca ttccgtttat tctgcaaagc cgataattct ttttgttttg
    10441 cggccagttc tgcttccagt tgcgttttct gttcaaatgc aatcgcaccg gcttttttca
    10501 attccgccga ttcggcaatc actttttcac gtcgttcgat cagcaatgtg atttccgctt
    10561 cgatcaattc cgattcttcc cgatatgcgg caatctgacg gcgcgctgca ttcagccgtt
    10621 catccagaat ggaagattca cggctgcttt cgtccatcgc gtgatgatac tttgagagct
    10681 gatcatgcag ctctcccaaa agaatacgct ttccgcgaat ttcttcctga accgcggcaa
    10741 tatcttgctc aatgaccgca tactcttgtt ggatttgctt ggttttggct tcctgtgatt
    10801 cgcagagttc catggcttca accagcgatt gttgggtttt atgccaatgg taaccgtacc
    10861 aatctttcag cagaacagtc aggtctttct tcattgttca tattccgcgg ctcgcttgga
    10921 ttggccttgt aaacttttca ggcgaggttc gagttctccc aaaatgtcat taatacgttc
    10981 caaattgtgt tgggtaattt caaggcgatt gatcgcttcc tcacgacgac ttctgaacaa
    11041 gccgattccg gctgcttctt caaagaagcg acgtcgatca tccggcttga gagatagggc
    11101 ggaatcgatt aacccttgcc cgataatggt gtaagttctt tccgataaac cggattgacc
    11161 taacagctcg ttgatatctt tcaatcggac acgctgtccg ttgacctgat attcgttttc
    11221 cccgctgcga taagctcgac gggtgatctc aacttcttcg taatcgatcg gcagccattc
    11281 ttcctgatta tcaaaaagga tggaaacgga agccatgctg gagcgagctt tttgatcgga
    11341 gccggtgaat atcatatcca tggtcttccg cccgcgcagg acggtataag attgctcccc
    11401 caaaacccaa cggattgcgt ccgctatgtt tg
//
LOCUS       DAOMIV010000008        10749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_6102_length_10749_cov_4.371704, whole genome shotgun sequence.
ACCESSION   DAOMIV010000008 DAOMIV010000000
VERSION     DAOMIV010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6102_length_10749_cov_4.371704"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKV59_00410"
     CDS             <1..311
                     /locus_tag="PKV59_00410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQP45240.1"
                     /translation="LDLGLNFIDLAKTNDPITGGDLDWVARGILVYPQLEDALFALQP
                     GSYSDIIETDIGFHILYAAERSTDHALTPQSRQILEHKILSDWVKEQKSSAEIIMLNK
                     "
     gene            323..1192
                     /locus_tag="PKV59_00415"
     CDS             323..1192
                     /locus_tag="PKV59_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45241.1"
                     /translation="MPTTRYDPDLLDRIDRKRKKKRRESCLSRLLLMILILSIIIAVF
                     FVWVLTQKNKKKPIETPGIETSWYEQTFDVLFSSITPTQTDTFTPTLPTPTVTQTNTM
                     TPTVSLTPTQTPTPRLRTRTPSGGNQLPTSTPLLLLNAAETEIANLKIAETSISASST
                     PAATPDMWFEIQGQPGATNAEIIYPNAECSWMGVAGLVNDARGDPIIGLYVQVGNFPD
                     GEIRETLTGLFPIYGESGYEITIARPVMEIPQPLWIQLLNESHVPVSEKVYFKPSNSC
                     ERSLILINFKRVR"
     gene            1365..2378
                     /locus_tag="PKV59_00420"
     CDS             1365..2378
                     /locus_tag="PKV59_00420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQP45242.1"
                     /translation="MKNKAKYLFQSSFLFFCLILLISGCNLKEKRDQKLATEIAVIST
                     EIQSTLQAAQTQTVASIPTDTPLPTFTPTPPATETPTPTVEFLPTATATPDVRPSADE
                     WANWPILPTLSENAKKIFWHGVNDLKTNPKVFSKIGDCQSRPNVFLGIYDMGYEGILA
                     DEDLYLQAAIDYYKGSFLMESYAVHDGMNVGSVLTTTWADPKACHKDENALECEIRIH
                     NPSIMFVNLGTNWVSGVSTDAYYDYLAEIVQVLIDHGILPVLSSKADNVEGDNSINRI
                     TAQVARDFDVPFFNFWLAAQSLENHGLSKEKPIYLSVSAWDYRNYYALKLLYNFGNEL
                     GLF"
     gene            2709..2780
                     /locus_tag="PKV59_00425"
     tRNA            2709..2780
                     /locus_tag="PKV59_00425"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2741..2743,aa:Lys,seq:ttt)
     gene            2865..3335
                     /gene="greA"
                     /locus_tag="PKV59_00430"
     CDS             2865..3335
                     /gene="greA"
                     /locus_tag="PKV59_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQP45243.1"
                     /translation="MTNTMLTREGYDKLVAELDYLRNVKRAEVSERLRNAIDGGGDDL
                     LENAEYEAAKNEQSFIEGRISDLEYLIAVAKIVDSPEKIGVADIGSKIKIQEEGSDEI
                     ETYTIVGAPEADPKENKISNESPIGKAVLGHHKGDIVQVSAPVGSYNVKIIDIE"
     gene            3371..4897
                     /gene="lysS"
                     /locus_tag="PKV59_00435"
     CDS             3371..4897
                     /gene="lysS"
                     /locus_tag="PKV59_00435"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQP45244.1"
                     /translation="MDFNELEKVRVQKIQKLKELGIPAYPTRSYISSTIKEACEAFEK
                     AESTGSDQPIEVTIAGRMRSLRIMGKLAFTHIEDGTGRIQLFLRVNELGDQLQFFKDY
                     FDIGDFVEAHGTVIRTRSGEISLQTTSIRMLAKAISPLPAAKEEVVDGVTVKHTGLSD
                     PEIRYRQRYADLVVNPEVRNIFRTRSKIVSALREFLDSHDFLEVETPVLQPIYGGAAA
                     RPFITHHNQLHQDLYLRISFELYLKRLLVGGLDRVYEIGRDFRNEGVSYKHNPEFTQL
                     EFYCAYFDYLKVMDFTEQMIRYAAEKALGTTKLKYGDVEFDLAKPWNRVNMREGILEK
                     TGIDFEKYPTTESLAAAMNEKGIQTKPGLQRGKLINQLVSDFLEPAFLQPTFLYDYPW
                     EVSPLAKRKPENPLITERFEGFIAGMELCNAFTELNDPIDQEARFLEMGRTYAEDDEE
                     RNPIDEDYLRAMRYGMPPCGGFGMGIDRLTMLLTNQSTIREVILFPHLRERDKDQEDN
                     "
     gene            4897..5574
                     /locus_tag="PKV59_00440"
     CDS             4897..5574
                     /locus_tag="PKV59_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQP45245.1"
                     /translation="MNFAVLWDLDGTISDSFQCFYMALERILSEQKPTIKITPEEYKS
                     QYFGATFPSILDRMYQGKLSDTQIWELNERYFKYSVEFARQGTVFPIPGVESFLKKLH
                     AADIRMAIASSSTVEMIFTELDVLGFISYFDNIISGNLLPSKPAPDVFQVAAASIRAQ
                     PSQCVVIEDSVDGITAAKNAGMRTIAVTTTNPADKFGQADRVTEDFRKFDLDALKSLF
                     VTDFSNL"
     gene            5945..6124
                     /locus_tag="PKV59_00445"
     CDS             5945..6124
                     /locus_tag="PKV59_00445"
                     /inference="COORDINATES: protein motif:HMM:NF039193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HQP45246.1"
                     /translation="MEIEGYCVKCKAKRTIQKPTAGFNKRGSAVVYGTCPVCGTKIYR
                     IGRTELHKGMEAPKK"
     gene            6204..6989
                     /locus_tag="PKV59_00450"
     CDS             6204..6989
                     /locus_tag="PKV59_00450"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQP45247.1"
                     /translation="MDTLELESFLSDLPMGGIKYFETIDSTNRYALEWAEKGAEDFSL
                     VVANEQTAGRGRMARKWLTTPGNSLAMSLILRPSHKEQKQIHLFSPLAGLAVAESLKN
                     TYGLNPLIKWPNDILLDHKKVCGILCEAAWDKDELRGMVIGIGLNLKNESIPTDDSLR
                     YPASSLESAGCDCLEVLPHIRNILLEFRRLRPILATSEFITKWESYLAFKGEQVILTA
                     PEKDPEIFTLIGLDAEGNLHVIDSAGDEKFFQAGEISLRPFKS"
     gene            complement(7060..8544)
                     /gene="gatB"
                     /locus_tag="PKV59_00455"
     CDS             complement(7060..8544)
                     /gene="gatB"
                     /locus_tag="PKV59_00455"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQP45248.1"
                     /translation="MAESQTGGFTIRGKRIMTEYEVTIGLETHIQLNTVTKAFCSCKA
                     DSWGEEPNTNICPVCTGQPGTLPRTNKAMVEKALMLCLAMNADINRKSFFDRKNYLYA
                     DLPAGYQVTQNDMPIGKGGYLDFFMDDGRKIRVHIDNLHMEEDAGKTKNEGDIRLIDM
                     NRCGVPLVEMVTKPDLHSADEAATYLMRLRQLLRWIGVSEANMEKAHLRCDANVSIRP
                     KGAKELGKKCEIKNINSIEAVRAAINSEVERQTRELNAGNKVEQWTIEWDNDAQELRK
                     MRSKETAADYRYFREPNLMPVVISEEFLEEVKAKMPELPIQRRERFINELGLPEYDAS
                     ILTGERDTSEFFEAACKIYGKDNKRVSNWMMNEVLRVVNEKGISVSELRITPQDLADI
                     ISLVDSKRINTSTGKTLVDKVEETGKSPAEIVEESGLGLVSDDSEIRKVCERIITDNP
                     KEVGLYKGGKETLIGWFVGQVMREMRGKADPVLTKSILEELLKQ"
     gene            complement(8511..10100)
                     /locus_tag="PKV59_00460"
     CDS             complement(8511..10100)
                     /locus_tag="PKV59_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HQP45249.1"
                     /translation="MTELTSMSALDQARLIREKKISAEELLNETLAQVKKVDGRPGSL
                     EEYIEQPEDKEKVHAFILITEESAREKARAVDRKVAAGEELGPLAGVPYSAKDVYCTK
                     GIQTTAASRILDGYHPPYTSTVIQRLEWADAVLFGKANLDEFTFGGSNETTAFKPTVR
                     NPWALDHVPGGSSGGSAAAVAANEGAISVGTDTGGSIREPAAFTGTVGLKPTYGRVSR
                     YGVIPFASSMDSMGPITRTVADAAAVLREIAGFDPNDSVTGRVPVDNYPEELKKGVKG
                     MRIGVSPDYMRITYLDENGNYSSTAIDPEIESCVRKALRIYKDLGAEIIEDVPMTNTK
                     YSVPAYFVITRIEAYSNLQRYDGLKYGHTTKRPVEDMYDLYYKSRGEGFGYQAKLRLL
                     TGLFASQEKFYEKYYLRAQRARAMLRNDFDEAFDPNGKYRLDVLLTPSTPTPAFQFGG
                     VTATDPLLMQFADQFTSPMNFAGTPGISFPCGLSSEGLPIGLQAAGYDYCESKILRAA
                     YAFEQATSDEDWRKVKPVVLR"
     gene            complement(10104..10496)
                     /locus_tag="PKV59_00465"
     CDS             complement(10104..10496)
                     /locus_tag="PKV59_00465"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45250.1"
                     /translation="MKRSYLQLFEDPFDKIPYKKGDFMDNDAITKEMLDDFVHVAEVE
                     LSTEEFESILAKMNDQLTVIHELTAIPLEGNLPPVIHGNPFPEEIQAPLREDVWIPFE
                     HPEDIISQAPRTQDGYIVSPDVPHQTLE"
BASE COUNT         2999 a         2442 c         2329 g         2979 t
ORIGIN      
        1 aactggattt ggggctgaat tttatcgatc ttgccaaaac caacgatccc attaccggcg
       61 gtgatttgga ttgggttgca cgaggcattt tggtttatcc gcaattggaa gatgcgcttt
      121 ttgccctgca accgggaagc tacagcgata tcatcgagac ggatatcgga ttccacatct
      181 tgtatgccgc ggagagatca acggatcatg cactgacacc gcagtcccgc caaattctcg
      241 aacacaagat cctatcggat tgggtcaagg aacaaaaatc ttccgcggaa atcatcatgc
      301 tgaataaata aagattctta aaatgccgac aacacgatac gatccggatc tccttgaccg
      361 gatagacaga aaaaggaaga aaaaacggag agaatcttgt ctgtctcgcc tgcttctgat
      421 gatattgatc ctttcgatca ttatcgccgt tttctttgtt tgggttctca cacaaaaaaa
      481 taaaaaaaag cccatcgaaa caccgggcat tgaaaccagt tggtatgaac agacttttga
      541 tgttcttttt tcttcgatta caccgactca aaccgacacg tttactccga cccttccgac
      601 tccgaccgtc actcagacaa atacaatgac tccgaccgtt tctcttactc cgactcaaac
      661 tccgacgccg agattaagaa cccgaacgcc gtccggcgga aatcagctcc ctaccagcac
      721 acctcttctg ttattaaatg ccgcggaaac ggaaattgcc aatttaaaaa tcgccgagac
      781 ttcgatcagc gcttcgtcta cgcccgctgc cacccctgat atgtggtttg aaattcaagg
      841 tcaacccggt gcgacgaacg cggagatcat ctatcccaat gccgaatgct cttggatggg
      901 ggttgccgga ttggtgaatg atgccagagg ggatcccatc atcggattgt atgtccaagt
      961 cgggaacttc ccagacggtg aaatccgaga aactctaacc ggtcttttcc ctatctatgg
     1021 ggaatccggt tatgaaatta ccatagcccg accggttatg gaaattccgc agcctctttg
     1081 gattcaatta ttgaatgaat cccatgtgcc ggtttcggaa aaagtttatt tcaaaccgag
     1141 caacagttgc gagcgcagtt tgatactgat caacttcaaa cgggtacgtt agaccgaaga
     1201 aaagcgaaaa atcacattgg atcatctatc ttctctttaa caagtcggaa tcgatgatca
     1261 acattaccaa agagatcctt tataaagatt tattcacaat ccgtcggaga agtatgaacc
     1321 gcccttgctt tgttttttaa ttttcattgt aaaatactta aggtatgaaa aataaagcaa
     1381 aatatttgtt tcaatcgtct tttctttttt tctgccttat tttattgatt tccggctgca
     1441 atctgaaaga aaaacgagat caaaagttgg caaccgaaat cgcggttatt tccaccgaaa
     1501 ttcagagcac attgcaagcg gcacagaccc aaaccgtcgc aagtattccg accgatactc
     1561 cgcttccgac cttcactccg accccacccg ctacggagac accgaccccg accgtcgagt
     1621 ttttgccgac ggcaactgcc actccggatg tacgaccttc cgcagatgaa tgggctaatt
     1681 ggccgattct tcccacttta tccgaaaatg cgaaaaagat tttctggcac ggtgtgaacg
     1741 atctgaaaac caaccccaaa gttttttcca aaatcgggga ttgccaatcg cgtccgaacg
     1801 tttttctggg gatatatgat atggggtatg aagggatttt agcggatgaa gatctttatc
     1861 ttcaggcagc cattgattat tataaaggat cgttcctgat ggaaagctat gccgttcatg
     1921 acgggatgaa tgtcggatcg gttttaacca ccacctgggc tgaccccaaa gcttgtcata
     1981 aagatgaaaa tgcactggaa tgcgaaattc gtattcataa tccgtccatt atgtttgtta
     2041 atctcggtac gaattgggtt agcggagtca gtacagacgc ctactacgat tacctggctg
     2101 aaattgtgca ggttttgatc gatcatggga ttctgcctgt tttgtccagc aaggcagata
     2161 acgtggaggg agacaacagt attaaccgga ttaccgcgca agtggccaga gattttgatg
     2221 tgcccttttt caatttttgg cttgcagcgc aaagtttgga aaaccatgga ctttccaagg
     2281 agaaacccat ttatttatct gtcagtgctt gggactatcg gaattactac gccttgaaat
     2341 tgctctataa cttcggaaac gaactgggac tcttttaatt catcgggtca gttgattaag
     2401 taaaaaacct ctgaaagatc agaggtttta ttatgaaaat ttttgccttc ttttcttttt
     2461 ccagaaggca ggaatgactc gagctttttt ccccctacta gctacaaagt gctgttttcc
     2521 aataattcac agcctttatt atagtttttt gaacaggctt gtcaagaggt tataatatta
     2581 aagttatatt aatcttttga atataaaagg atgcatttta aacgaatcgt tattcgaaat
     2641 gatacttatt ttcaccggct tattgacaca agataatcgg catgataaaa tgtacgagtt
     2701 acagtcaagg gcgtatagct caatggtaga gcagtggcct tttaagccat ttgttctggg
     2761 ttcgagcccc agtacgctca ctcggccgga aattgctctc ctcactcgag gagggtattt
     2821 ttgttaaatt ttaaattttt atattctttt agatcgaggt cattatgaca aacacaatgc
     2881 taactcgcga aggctatgat aaactggtcg cggaattgga ttatctgcga aacgtaaaaa
     2941 gagctgaagt ctcagaaaga ctccgaaacg ctatcgatgg cggcggagat gatcttcttg
     3001 aaaatgcgga gtacgaagcc gcaaagaatg agcaatcatt cattgaaggc agaatcagtg
     3061 atttggaata tttgatcgcg gtggccaaga tagtcgacag cccggaaaaa atcggcgtcg
     3121 cggatatcgg ttcgaagatc aaaattcaag aagagggtag cgacgaaatt gaaacttata
     3181 cgattgtcgg agcaccggaa gcggacccca aagaaaataa aatctcgaac gaatccccga
     3241 tcggaaaagc ggtgttggga caccataaag gggatatcgt ccaggtctcc gctcccgtcg
     3301 gttcttataa cgtaaagatt atcgatatcg aataaactac ggcaaatctc attctgaaat
     3361 aaagggaatc atggatttta atgaattaga aaaagtcaga gtacaaaaaa ttcaaaaact
     3421 gaaagaatta ggcatcccag cctatccgac tcgttcttat atcagttcaa caattaaaga
     3481 agcatgtgaa gcgtttgaaa aagcggaatc gacaggatcc gatcaaccga tcgaagtgac
     3541 gatcgccggt cgaatgcgtt ccttacggat tatggggaaa ttggcgttta ctcatatcga
     3601 agacggaacg ggaaggattc agctcttttt gagagttaat gaactgggtg atcagcttca
     3661 gttttttaaa gattatttcg atatcggtga tttcgtcgaa gcccacggga ccgttatcag
     3721 gacgcgatcg ggtgaaatat cgcttcagac aacttccatt cggatgctcg ccaaagctat
     3781 ttcgccgctt cccgcagcaa aagaagaggt agtcgatgga gtgaccgtca agcataccgg
     3841 gttgagcgat ccggaaatcc gatatcgcca acgatatgcc gatttggtgg tgaaccccga
     3901 agtccgaaat atcttccgaa cgcgttcaaa gatcgtttcg gcgctgcgag aattcttaga
     3961 cagtcacgat tttttggaag ttgaaacccc tgtattgcaa ccgatctatg gaggagctgc
     4021 cgccagacct ttcatcactc atcataatca gcttcatcag gatctttatt tgcgtatatc
     4081 gtttgagtta tatctaaaaa ggttgttggt cggcgggctg gatcgcgtct acgagatcgg
     4141 ccgtgatttt cgcaacgagg gcgtctctta taaacacaat ccggaattta cacagctcga
     4201 attttattgt gcttattttg attatctgaa agttatggat ttcacggaac agatgatccg
     4261 ttatgccgca gaaaaagcac tgggaacgac caaattgaaa tacggcgatg ttgaatttga
     4321 tttggcaaag ccctggaatc gggtaaatat gcgggaaggg attctggaaa agaccggaat
     4381 cgatttcgaa aaatatccga caaccgaatc tttggcagct gcaatgaatg aaaaggggat
     4441 tcagaccaag ccggggttgc aacgcggaaa attgatcaat caattggtaa gcgatttttt
     4501 ggaaccggct ttcctgcaac ctacttttct atacgattat ccgtgggaag tatccccttt
     4561 ggcaaaaaga aaaccggaaa atccgctcat caccgagaga tttgaaggat ttattgccgg
     4621 aatggaactt tgtaacgctt ttacggaatt gaatgatccg atcgatcagg aagctcgttt
     4681 tctggaaatg ggaagaactt atgcggaaga tgacgaggaa cgaaatccga ttgatgagga
     4741 ttatttacgt gcgatgcgat acggcatgcc tccctgcggc ggtttcggaa tgggaattga
     4801 tcgtttaaca atgctactga ccaaccaaag cacaattcgc gaagttattt tatttcccca
     4861 tttgcgtgaa cgagataagg atcaggaaga caactgatga attttgccgt tctctgggat
     4921 ttagacggaa caatttcgga ttcgttccaa tgtttttata tggcattgga acgaattttg
     4981 agtgaacaga aaccgacgat caaaataaca cccgaagaat ataaatctca gtatttcggc
     5041 gcaacttttc ccagcatttt ggatagaatg tatcaaggaa aactctccga tacacaaatt
     5101 tgggaattaa acgaacgcta tttcaaatat tccgttgaat tcgccagaca gggaaccgtc
     5161 tttccgatcc cgggtgtcga atctttcttg aaaaagttac acgccgctga catcaggatg
     5221 gccatcgcct catccagcac ggtcgagatg atttttaccg aattggatgt tctgggattc
     5281 atttcctatt tcgacaatat tatttcgggg aatttgttgc cgtccaagcc ggctccggat
     5341 gttttccaag tcgcagccgc atccatccga gcgcaacctt ctcaatgcgt tgtgatcgaa
     5401 gattctgtcg acggtattac agccgcgaag aatgcgggca tgcgcaccat tgccgttaca
     5461 accaccaacc cggccgataa gttcgggcag gctgatcggg tgacggaaga ttttcgaaag
     5521 ttcgatctcg acgcgttaaa atcccttttt gtcactgatt tttcaaattt atgacagtaa
     5581 taccgaattt atgacattcg taatattcag attccttttt ggaaacgaat tttctttttt
     5641 tttaatcagg atcctaaata attcgatcta aaggaagctt tttttggctc atttcctcgt
     5701 aaaaatgacc gaatcggatt ttccggaata atttcaacca agccgtcaat atcatcattc
     5761 tttgaaattt ctctaaaaaa gtcaattttt acgcaaaaaa tacgtcatca gtaagccatt
     5821 ttggcttgac aaatataccc atttttttaa actattgata tacttttgaa ttagtcaagc
     5881 ggaataggta gaagcgcatt ttattccgtt ctaattctga aagaaattta ttatggaggt
     5941 aataatggaa atcgaaggat attgtgtaaa atgcaaggct aagcgtacta ttcagaaacc
     6001 taccgctggt ttcaataaac gaggcagcgc agttgtatac ggtacctgcc cggtttgcgg
     6061 tacaaagatt tatcgaatcg gtcgtaccga attacataaa ggaatggaag ctcccaaaaa
     6121 atagagcttt ctaaaaaaga aaatgaagct gccatgcgtt tcgtatggca gtcattattt
     6181 aaaagggaaa tttcaaccga aatatggata ctttggaact cgaatccttt ctctctgatt
     6241 tgccgatggg aggaatcaaa tattttgaaa ccatcgactc caccaatcgc tatgcattgg
     6301 aatgggcaga aaaaggtgcg gaagatttca gtttggtagt cgcaaatgaa caaactgccg
     6361 gccgaggccg gatggcaaga aaatggctga caacgcctgg caacagtctt gcgatgagcc
     6421 tgattttgcg gccgagccat aaagaacaaa aacagattca tctgttttca ccattagccg
     6481 gattggcagt tgcggaatca ttgaaaaata cctatggact gaatccgttg attaaatggc
     6541 cgaatgacat tctgttggat cataaaaagg tttgcggtat tctctgcgaa gctgcatggg
     6601 ataaagacga gttaagaggc atggtcatcg ggatcgggtt aaacctgaaa aacgaatcga
     6661 tccccacaga tgacagcctt cgttaccctg cgagttcctt ggaatcggca ggttgtgatt
     6721 gcttggaagt tttaccgcat atccgtaata ttttgcttga attccgccgt ttacgtccga
     6781 ttctggcaac ctctgaattc atcacaaaat gggaaagcta cctggcattc aaaggagaac
     6841 aagttatcct aacagccccc gaaaaagacc ccgaaatctt cacattaatc ggattggatg
     6901 cagagggcaa tttgcatgta attgattccg ccggagatga aaaattcttc caagccggtg
     6961 aaatttccct cagacctttc aaatcataaa aaacccctga cgaaacaaaa aaggaaaaga
     7021 accgatcttt tccttttttg ttttgaattc atgaaggagt tattgtttca ataattcttc
     7081 gaggatcgac ttcgtcaaga cagggtccgc tttcccgcgc atctcccgca tgacctgacc
     7141 gacgaaccag ccgatcaatg tttctttccc gcctttatac aaacccactt cctttggatt
     7201 atccgttatg atacgctcgc aaactttgcg gatttcgctg tcgtcactca caagaccgag
     7261 ccccgattct tcgacaatct ctgcgggaga tttgccggtt tcctcaactt tatccaccaa
     7321 cgtcttgccg gtcgatgtat tgattctttt ggaatcaacc aacgaaatga tatccgccag
     7381 atcctgcgga gtgattctca actcggaaac cgagatacct ttttcattga caactcgcag
     7441 aacttcattc atcatccaat tcgaaacacg cttattgtct ttcccgtaaa tcttgcaagc
     7501 cgcttcgaag aattcggagg tatcccgttc tccggtcaga atagaggcat catactcggg
     7561 taaacccaat tcattaataa accgttcacg ccgttggatc ggaagctccg gcatttttgc
     7621 tttcacttct tccaggaatt cttcggaaat gacaacaggc atcaggttcg gctcacgaaa
     7681 ataacgataa tccgccgctg tttctttgga acgcattttc cgcagttcct gagcatcgtt
     7741 atcccactcg attgtccatt gctcaacctt gtttcccgca ttcaattcac gggtctgccg
     7801 ttcaacctcg gaattaatcg ctgcccgaac ggcttcgatc gaattgatat tttttatctc
     7861 gcattttttc ccaagttctt tagcgccttt cggtcgaatc gagacattgg catcacagcg
     7921 taaatgggct ttttccatat tcgcttcgga aacaccgatc caacgcaata actgtctcag
     7981 acgcatcaga taggtggctg cctcgtcggc agaatgtaag tccggtttcg tgaccatctc
     8041 aaccaggggt acgccgcacc gattcatatc gatcagacgg atatcgcctt catttttcgt
     8101 ttttccggca tcttcttcca tgtgtaaatt atcgatatga acgcgaattt tccttccgtc
     8161 atccataaaa aagtctaaat atccgccttt accgatcggc atatcatttt gggtaacttg
     8221 gtatccggcc ggaagatccg cataaaggta attctttcga tcaaagaaag atttcctgtt
     8281 gatatccgca ttcattgcca gacacagcat cagagctttt tcgaccattg ccttattggt
     8341 ccggggaagt gtgccgggct gcccggtgca aacgggacaa atattggtat tgggttcctc
     8401 tccccatgaa tcggctttgc agctgcaaaa agctttggtt acggtattta attggatatg
     8461 ggtttcaagc ccaatagtta cttcatattc ggtcatgatt ctcttccccc ttatcgtaaa
     8521 accaccggtt tgactttccg ccaatcttcg tccgacgtcg cctgttcgaa agcgtaagcc
     8581 gcacggagta ttttggattc gcagtaatca tatccggcag cttggagtcc gatcggtaga
     8641 ccttcggaac tgagtccgca gggaaaactg attccgggag taccggcaaa attcatcggc
     8701 gaagtgaact gatcggcaaa ttgcatcaga agcggatcag tggcggtgac cccaccgaat
     8761 tgaaatgccg gtgtcggcgt agacggtgtc aataacacat ccaagcgata tttgccattc
     8821 ggatcgaagg cttcgtcgaa atcattacgt aacatggcac gtgctcgttg ggcacgcaaa
     8881 tagtactttt cataaaattt ttcctgactg gcaaaaagtc ccgtcaggag acggagtttc
     8941 gcttgatacc cgaagccttc cccgcgggat ttgtagtaca aatcatacat atcctcaacc
     9001 ggtcttttcg tggtatgccc gtatttgagc ccgtcatatc gttgtaaatt tgaataagcc
     9061 tcgattcggg ttataacgaa atacgccgga actgaatatt ttgtattggt catcgggacg
     9121 tcttcgatga tttcggcgcc caaatcttta tagattcgca gagctttcct gacacaggat
     9181 tcgatttcgg gatcgatcgc cgtggaagaa taattgccgt tttcatccaa ataagtaatc
     9241 ctcatataat ccggagaaac gccgattcgc atccccttaa cgcctttttt cagctcttcc
     9301 ggataattat ccacgggtac tcttcccgtg acactgtcat tcggatcgaa cccggcgatt
     9361 tcccgtaata cagctgcggc atcggcaacc gtgcgggtga tcggtcccat cgaatccatt
     9421 gaagaggcaa acggaatcac accgtaacgt gaaacgcgac cgtaagtggg tttcaatccc
     9481 acggttccgg taaatgccgc cggttcgcga atagacccac cggtatcggt gcccacagaa
     9541 atggcgcctt catttgcagc aaccgctgcc gcggaaccgc ctgacgaccc tcccggaaca
     9601 tgatctaaag cccacggatt gcgcacggtc ggtttaaatg cggttgtttc gttggatccg
     9661 ccaaaagtaa attcatccaa attcgctttt ccgaacagca ccgcatccgc ccattccaac
     9721 cgctgaataa ctgtggaagt gtaaggagga tgatacccat ccagaatcct ggatgcggcc
     9781 gtcgtttgaa tcccttttgt gcaatagaca tctttcgccg aataaggaac accggctaag
     9841 ggtcccaatt cctctccggc ggctactttc cgatccaccg ctcgggcttt ttcgcgtgcc
     9901 gattcttccg tgatcaaaat gaacgcatgc actttttctt tatcttccgg ttgctcaata
     9961 tactcttcca atgagcccgg acgaccatcc acttttttta cttgcgccaa cgtttcgttc
    10021 agaagttctt ccgccgatat ttttttctct cggatcagtc ttgcttgatc caaagccgac
    10081 atactcgtta attcagtcat gatttactcc aatgtctgat gtggaacatc cggcgaaaca
    10141 atatatccgt cttgtgtcct cggtgcttgg ctgatgatat cttccggatg ctcaaaagga
    10201 atccaaacat cctctcgcaa cggtgcctgg atttcttccg gaaacgggtt cccatgaatt
    10261 acagggggta aattgccttc taacggaatg gcggtcagtt cgtgaattac cgtgagctgg
    10321 tcattcattt tggctaaaat tgactcaaac tcttcggtgc ttaattcaac ttccgcaaca
    10381 tgcacaaaat catcgagcat ttcttttgtt atcgcatcat tgtccataaa atcacctttt
    10441 ttatatggaa ttttgtcgaa gggatcttcg aataattgca aataggacct tttcaagcaa
    10501 tcaattccta attctaccaa ataaagatgt gattaacaga taaaagattc aggttcagtg
    10561 tcataaattc acaagcatca ttcgataaaa gcagtgccga taccgggtcg ttttttttac
    10621 tcattattcc aaaggatgat ctaaaagttc tatgctttgg gaaaaatcaa cggaacggaa
    10681 aaaattgaaa tgttataatc accccaatgg aaacccctca taataacttg aagaatatcc
    10741 gaaaagttc
//
LOCUS       DAOMIV010000009        10547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_6257_length_10547_cov_4.712162, whole genome shotgun sequence.
ACCESSION   DAOMIV010000009 DAOMIV010000000
VERSION     DAOMIV010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6257_length_10547_cov_4.712162"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKV59_00470"
     CDS             <1..664
                     /locus_tag="PKV59_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45251.1"
                     /translation="RKKSKYITAISFAILIAGAVAAFSPQYFTWSFIALIAGVILSQI
                     SISMTTRWGREPSNNNIFNVKLKGLSDNFSIYHYMDPVDHLLVGTAGAFILMPYFQGG
                     VIGYDEKKQRWTQKKASMFMKIFGQESLGRPDLDAEANLEAVKKYFISRGIDFPEDKI
                     HPILVFLGPKSSIDPNANFKYDTIMLDKLKDTIRKYAKEDRLSPEFIDEVTDKLPLDD
                     IE"
     gene            complement(793..1185)
                     /locus_tag="PKV59_00475"
     CDS             complement(793..1185)
                     /locus_tag="PKV59_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HQP45252.1"
                     /translation="MDDLYRENILDHYKNPIYKGTLEPADLSFEDENPLCGDHLRIDL
                     RVDEKGNVTDARFSGHGCAISQASADLLLESIIGKNIEDLKKLTKEDILDLLGIEELG
                     PVRLKCALLSLKVLKGSLYGIGQKDEDL"
     gene            complement(1187..2416)
                     /locus_tag="PKV59_00480"
     CDS             complement(1187..2416)
                     /locus_tag="PKV59_00480"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQP45253.1"
                     /translation="MDEKQIIQDFPIFSQLDRNGKTLCYLDSAATTQKPLSVLEAMDT
                     YYKTYNANIHRGVYHISELATEAHETTREKVRAWIHARSTTEIIFTRNTTESINLVAY
                     SWGRKNLNAGDHIILTEMEHHSNLVPWQILAEEKGVELDFIPITDAGELDLEVYRQLL
                     EKHPKLVCATGMSNVMGTIPPSKEMISAAHAAGALFLLDGAQMVPHMQVNIRDLDADF
                     FAFSAHKMLGPTGIGVLYGKESLLEVMPPFLGGGDMIKKVHLRSFSANDLPYKFEAGT
                     PAIAEAIGFGAAIDYLAQIGMDAVFEHESEITRYAIHELMKIPGLKIIGPLTSSNRGS
                     AVSFTLDYVHPHDVAQILDRENICVRAGHHCAMPLHERCNVDASTRASFYIYNSLTDV
                     DRLIDGLKIVHQLFGQD"
     gene            complement(2439..2705)
                     /locus_tag="PKV59_00485"
     CDS             complement(2439..2705)
                     /locus_tag="PKV59_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45254.1"
                     /translation="MGAIPTMREPIPEVNYEVGDLVEVLCDHDRKGVRVNGWLKGIVV
                     QVDNKMIAVQFRTNVYLTDGWMVPDHILWYPLNSPNIRPYQKKA"
     gene            complement(2750..3979)
                     /gene="sufD"
                     /locus_tag="PKV59_00490"
     CDS             complement(2750..3979)
                     /gene="sufD"
                     /locus_tag="PKV59_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HQP45255.1"
                     /translation="MISNNQQNLSVQSNYEYLFKLIQSADDSNLDRIKTQTAEMLAEN
                     EYTTAEGSPQTLTDANERVLAGRFLWEDGVRSTGLDSNTRKQGVLLDTLENIRANQPE
                     IFLKIKYWQESLQQNPLEALIGSIAKETIVLYVPKFAVVSKELLLDVVLSQPGRNALM
                     RFWVFLEDGAQAVFTINLRSRKAEGENFYTGCLQVRVGDGAHLILNEVQDFHQKVYVT
                     ATKSVTTGKVSNIQWNTCELGSIRLRNNATMNLKGEGSNGLIYGLYFATDHQDIEMQT
                     RQNHLAPNTTSFLHFKGAINDDAYANWQGMIYVDPAAKKTDGYQKNENLMLSPNARVF
                     TKPGLEIVTDDVKCSHGTTVSEIDADQIFYLMSRGMSKNESELLILQGYFDAILNNIT
                     YDPIREKLKEKIYLKMI"
     gene            complement(3987..5402)
                     /gene="sufB"
                     /locus_tag="PKV59_00495"
     CDS             complement(3987..5402)
                     /gene="sufB"
                     /locus_tag="PKV59_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HQP45256.1"
                     /translation="MDNPQKYQLQGIDDADYEYGFQDPDISVFRTEAGLNEKVVRQIS
                     AMKEEPEWMLEFRLKSLKHFLERPMPAWGPDLSELDLDHLVYYVRPTEKPQNNWDEVP
                     STIKNTFNKLGIPEAEQKFLAGVGAQYESEMVYHNLQEHLSKQGVIFLSIEDGLKQYP
                     DLFRKYFGTVVPYNDNKFAALNSAVWSGGSFVYIPKGVHVDLPLQAYFRLNSANIGQF
                     ERSLIIADEGSQVHYVEGCTAPQYSTNSFHSGVIEIIVGRNARVRYSTIQNWSTNVYN
                     LVTQRAKVFAHGTMEWVDSNLGSKVTMKYPACYLMEDGAHGEILSMAFAGKGQIQDAG
                     SRMIHLAPNTTSKVTSKSISKNGGRSSYRGYMKINKNAENAKSNVVCDALILDDHSQS
                     DTYPTMDIDQQKVSVGHEASVSKIGEEQLFYLMSRGLSEEDATTMVVSGFIEPLVKEL
                     PMEYAVEMNRLIQLQMVDSIG"
     gene            complement(5425..6195)
                     /gene="sufC"
                     /locus_tag="PKV59_00500"
     CDS             complement(5425..6195)
                     /gene="sufC"
                     /locus_tag="PKV59_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQP45257.1"
                     /translation="MIGLEVKDLSVTVHDKKIINHLSIQVPKGEIHAIMGPNGTGKST
                     LSYTIMGHPAYRPDEGEILLNDQLINDWPPDKRSHAGLFLALQYPVAIPGVTVANFLR
                     TALNIHRKSKNPDDHGIPIPEFRKLLKEKMKMLKMDPDFAGRYLNDGFSGGEKKRTEV
                     LQMAILEPEIAILDEIDSGLDIDALRIVSEGINILVDQTHMGVLIITHYQRLLDYIKP
                     QAVHVMMDGQIVRTGDANLALELEEKGYDWIRQEQTTA"
     gene            6526..7872
                     /locus_tag="PKV59_00505"
     CDS             6526..7872
                     /locus_tag="PKV59_00505"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQP45258.1"
                     /translation="MRIIFLRHGEAIERGSVDDEQRFLTERGKRDLESNLPALAAYLR
                     NRNNVEIWSSPKIRAIETAQILTSGMENKDIRFKDFIGDGDTEQLKSELEKTGAETVV
                     IVGHQPYLSEWVDELCDSEILFQKGAAAEIEYYPDSDQKGKVLWHFPLKGLKQLARMT
                     GPEVEGNSFRQDLMIKMEQNFNQIAENRNRFLSEPDDVESAHQLRVSIRSFRSLISLG
                     KKYLKPEKYQDYQDQFRQAAAEISTLREIDVIINTWNEHLLDQKLDPSQSVLIQELNR
                     KRNIEKDRVIKIVSKNKYIDRLTKSSNDLIKTIRKSSEKDVPTQKVIDARLDKWYQYI
                     LKRMINYNEFNFSYIHPIRLKCKKYRYVSENFYGILDLEHRKRHRDVKQLQTLLGNTC
                     DAIRNQEAILKIIGLRSPALEQEVIAFINYEKEIESNYRKLFDSREWLQQISEKEN"
     gene            7999..8826
                     /locus_tag="PKV59_00510"
     CDS             7999..8826
                     /locus_tag="PKV59_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQP45259.1"
                     /translation="MNEIFEKFSMKDQTAVITGGGGLLGKEFCKTLAQAGATIVAADL
                     NLNLARQTVELIQEFGGHGIACAVDVTDPESVKAMVDLAVNETGRLDVLVCSAALDPK
                     FDGTQGKHSNTFEEYPLKQWRDALDVNLTGLFLCAQASVKPMIAQNHGVIINICSTYG
                     LVGPDQRIYERPDGDPSSRQYKPVYYSVTKAGVLGLTKYLATYYAGTGIRANCLTPGG
                     VYNGHDEFFVKQYSARTVMGRMANIDELSGALLFLASDASSYMTGSNLVVDGGWTAW"
     gene            8820..10073
                     /locus_tag="PKV59_00515"
     CDS             8820..10073
                     /locus_tag="PKV59_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HQP45260.1"
                     /translation="MVAKHAEVLAIIPARGGSKGIPHKNIKKFAGYPLIAWSIAAAKQ
                     SASVTRTIVSTDDEEIARVAREWGAETPFLRPAEFAMDQSLDLPVFRHALEWLKENEG
                     YEPDIVVQLRPTSPVRPVNLVDDAVRLLTDHPEADSVRGVVPSSENPFKMWLIDSETG
                     TMSGLIKLDGIPEPYNAPRQVLPATYWQTGHIDAIRPRRTFMAGDSMSGKVILPLLLD
                     PAFKVDIDTLADWDRYEQLVYYGGLDMVYPEHKRRSLPQKTCLLVMDFDGVLTNDEVI
                     VNQDGLESVICNRGDGIGFWLLREAGITAIVISSETNPVVRKRCEKLGIQSYQGTLDK
                     ATVLKKYLEENKIPAENTVYIGNDINDLQCFPLVGCAAAPADAHDLVKSKADIILKRN
                     GGQGVVRELCELIVRRNKESYGKDS"
     gene            10057..>10547
                     /locus_tag="PKV59_00520"
     CDS             10057..>10547
                     /locus_tag="PKV59_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HQP45261.1"
                     /translation="MGKTVKLGNRLVGEGQPVYVIAEIGINHNGDIGIAKRMIRSAYA
                     AGVDAVKFQKRTPAVCVPLSEQSKMRETPWGYMSYLEYREHVEFWEDEYHEIDELCHE
                     LGIDWFASVWDVESIDFLMKFDPIAYKVPSASLTDHELLDKLLSTRKPIILSTGMSTM
                     DQIR"
BASE COUNT         2933 a         2261 c         2398 g         2955 t
ORIGIN      
        1 tcggaaaaaa tccaaatata taaccgccat atcttttgcg attttgatag ccggtgcggt
       61 tgcggcattt tctccgcaat atttcacctg gtcttttatc gctttgatcg cgggtgttat
      121 tctttcgcaa atatccattt ctatgacaac ccgctggggc agggaaccga gcaataacaa
      181 tattttcaat gttaagctca aaggactgag cgataatttt tcaatttatc actacatgga
      241 tccggttgac catttgctgg tcggaacagc gggcgctttt attctgatgc cctattttca
      301 aggcggtgtg atcggctatg atgaaaaaaa gcaacgttgg acacagaaaa aagccagtat
      361 gttcatgaaa atattcggcc aggaatcttt ggggagaccc gatttggatg ccgaggcaaa
      421 tctggaagcg gtgaaaaagt attttatcag tcgcgggatc gattttcccg aagataaaat
      481 tcatccgatt ttagtttttc tcggaccgaa atcatcgatc gatccaaatg caaatttcaa
      541 atatgataca attatgctcg ataaactcaa ggatacaatc cgaaaatacg ccaaagaaga
      601 tcgactgtca ccggaattta tcgatgaagt gacggataaa ctgccgttgg atgatatcga
      661 ataatacttc aacgtaaaat tcgaaaagat tttgttggaa atctataaaa atcgtgcgga
      721 aatagcttcc gcacgattta ttttataaat attgatttga aaacggaggg gccggataaa
      781 agactttgat ttctataaat cctcgtcttt ttgtccgatt ccatacaaac tgcctttcaa
      841 aacctttaga gataataaag cgcattttaa tcggacaggc cctaattcct caattccaag
      901 taaatccaag atatcttctt ttgtcagttt cttgagatct tcgatatttt tcccgataat
      961 ggattcgagt aataaatcag ctgaagcctg tgaaatagcg catccgtgac cgctgaatcg
     1021 ggcatcggtt acatttcctt tttcatccac ccgcaaatcg attcgcagat ggtccccgca
     1081 caaagggttt tcatcttcaa aactcagatc cgccggttcc aacgtccctt tataaatggg
     1141 attcttataa tgatccaaaa tattttcgcg atataaatcg tccatttcag tcctgtccaa
     1201 aaagctgatg tacgattttc aagccgtcta ttaaacgatc gacatcagta agcgaattgt
     1261 aaatataaaa actggcccgg gtcgaagcgt caacattgca tcgttcatgt aacggcatgg
     1321 cgcagtgatg tcccgcgcgg acacaaatgt tttctctgtc caatatttga gccacatcat
     1381 gaggatgaac ataatctaaa gtaaaactga ctgccgatcc ccgattggaa gaagttaaag
     1441 gtccgatgat tttcagcccc ggaattttca tcaactcatg aatggcataa cgggttattt
     1501 ccgattcatg ctcaaagacc gcatccatgc cgatttgagc caaataatca attgccgctc
     1561 caaacccgat cgcttcggcg atggcgggtg ttcccgcttc aaacttataa ggcaagtcat
     1621 ttgccgaaaa ggatcgcaaa tgaactttct ttatcatgtc acctcctccg aggaaaggcg
     1681 gcatcacctc caacagcgac tctttaccat ataagacgcc gattccggtc ggtcccaaca
     1741 ttttgtgcgc cgaaaaagca aaaaagtctg cgtccaaatc ccggatattc acctgcatgt
     1801 gcggaaccat ctgggcaccg tctaatagaa aaagggcacc tgcggcgtga gcggcggaga
     1861 tcatttcctt ggagggtgga atagtcccca ttacgttgga cataccggtt gcgcagacca
     1921 gtttcggatg cttttcaagg agctgccggt aaacttcgag atccaattcc cccgcatccg
     1981 ttatcggaat aaaatccagt tcgacgcctt tttcctctgc caatatctgc cagggaacca
     2041 ggttggaatg atgctccatt tcggtcagga taatatgatc gccggcattc aaattttttc
     2101 ttccccagct ataggctacc aaattaatgg attcggtggt atttcgcgtg aatataatct
     2161 ccgtcgtcga tcgggcatga atccaagccc gaactttttc acgcgttgtt tcatgagctt
     2221 ccgtcgccaa ctccgagata tggtaaaccc cccgatgaat attggcattg tacgtcttat
     2281 agtaagtatc cattgcttcg agaacggata acggcttctg tgtcgtggct gccgaatcca
     2341 gataacaaag tgtttttccg tttcgatcga gttgactaaa aatcggaaag tcctgaataa
     2401 tttgtttttc gtccattctt aatccttttc tattaatttc aggctttttt ctgatatggt
     2461 cgtatattcg gcgaattcaa cggataccaa agaatatggt ccggcaccat ccatccgtct
     2521 gtcagataaa cgtttgtccg gaattgcacg gcaatcattt tattgtccac ttggacaaca
     2581 atgcctttca accagccgtt aactcggacg ccttttcgat cgtggtcaca taagacttcg
     2641 accaaatcgc ccacttcata atttacttcg ggaatcggct cccgcattgt cggtatcgca
     2701 cccactttaa cctccggaat cgaacggaaa aggaatagtc ccagttcgat taaatcattt
     2761 tcaaatatat tttctctttc aatttctccc gtatcggatc ataggttata ttattgagaa
     2821 tagcgtcaaa atatccttgc aggattaaaa gttcggattc atttttggac ataccgcggc
     2881 tcatcaaata aaaaatctgg tccgcatcga tctcactgac cgtcgttccg tgagaacatt
     2941 taacgtcgtc ggtgacaatc tccagaccgg gtttcgtgaa gactcgggca ttgggagaga
     3001 gcatcaggtt ttcattcttt tgatagccat cggttttttt agcggcggga tcaacataaa
     3061 tcattccctg ccaattggca taagcgtcat cattgatggc tcccttgaaa tgcagaaaac
     3121 tggtagtatt cggcgccaaa tgattctggc gggtttgcat ttcgatatcc tgatgatcgg
     3181 ttgcgaaata taacccataa ataagtccgt tcgatccttc ccccttcaga ttcatggtgg
     3241 cgttgtttcg aagtctgatc gagcctaatt cacaggtatt ccattgtata ttgctcactt
     3301 taccggttgt aacggatttt gtggcggtga cataaacctt ctgatgaaaa tcctgaactt
     3361 cattcagaat taaatgggct ccgtcaccga ccctgacctg taagcagccg gtataaaaat
     3421 tttcaccctc cgctttacgc gaacgcagat tgatggtaaa taccgcttgt gctccgtctt
     3481 ccaggaaaac ccaaaaacgc atcagtgcgt ttcgaccggg ttgcgagaga acgacgtcca
     3541 acagcaactc tttggataca accgcaaatt tggggacata caagactatg gtctctttgg
     3601 cgatactgcc gatcaacgct tcaagcggat tctgctgaag cgattcctgc cagtatttga
     3661 tttttaggaa tatttccggc tggtttgctc ggatattctc taatgtgtcc agtaaaacgc
     3721 cctgctttcg ggtattactg tccaagcctg tgctgcgaac gccgtcttcc caaagaaagc
     3781 gtccggcaag aactctttca ttggcatctg tcagggtttg agggcttcct tcagccgtcg
     3841 tatattcatt ttcagccagc atttccgcag tttgtgtttt gatccgatcg aggttggagt
     3901 catctgccga ttgaatcagt ttgaacaaat actcataatt tgattggact gatagatttt
     3961 gttgattgtt tgatatcatt tttgcctcac ccaatcgaat ctaccatttg aagttgtatc
     4021 agacgattca tttcaaccgc atattccatg ggaagttcct tgacaagcgg ttcgataaaa
     4081 ccggaaacaa ccatcgtggt ggcatcttct tcacttagtc cgcgactcat gagataaaac
     4141 agctgttctt ccccgatttt tgaaaccgaa gcctcatgtc cgacggatac tttctgctga
     4201 tcgatatcca tggtcggata ggtatcggat tgagagtgat catccaagat taaagcatcg
     4261 cataccacat tcgatttggc attttcagca ttcttgttga tcttcatata accgcgatag
     4321 gatgagcgtc caccattttt gctgattgat ttcgaagtga ccttgctggt ggtattgggt
     4381 gccaaatgaa tcattctcga tcccgcatcc tggatttgtc ctttgccggc aaatgccatg
     4441 gaaaggattt caccgtgtgc accgtcttcc attagataac aggcgggata tttcatggtc
     4501 actttcgatc ccagattgga atccacccac tccattgtcc cgtgtgcgaa aacttttgcc
     4561 cgttgagtga ctaagttata aacatttgtc gaccaatttt ggatggtgga ataacggaca
     4621 cgggcatttc tgccgacaat tatctcaatc acaccgctgt gaaaggaatt tgtggaatat
     4681 tgcggagccg tacacccctc gacatagtga acttgcgaac cctcatcggc gattattaag
     4741 gaacgttcga actgaccgat attcgctgag ttcagtcgaa aataagcttg aagaggcagg
     4801 tcgacatgga cacctttcgg gatataaaca aatgatccgc ctgaccaaac ggcgctgttt
     4861 aaagcggcga atttattatc gttataaggg acaacggtcc cgaaatattt tctgaacaaa
     4921 tcgggatatt gtttcaaacc gtcttcgata ctcaggaaga taaccccttg tttggaaaga
     4981 tgttcctgaa gattatgata gaccatttcc gattcgtatt gtgcaccgac tccagccaga
     5041 aatttctgtt cggcttccgg aattcccagt ttattaaagg tatttttgat ggtggaaggg
     5101 acttcatccc aattattttg cggtttttcg gtcggacgga cataatagac gagatgatcc
     5161 aaatccaatt cggacaaatc cggaccccaa gccggcatcg gccgctccaa aaaatgtttc
     5221 aaacttttaa gccggaattc caacatccac tccggctctt ccttcattgc ggaaatctgg
     5281 cggacaactt tttcatttaa tccggcttcc gttcgaaaga cggaaatatc cggatcctga
     5341 aatccgtatt cgtaatcggc atcgtcgatt ccctgtaact gatatttttg tggattatcc
     5401 attctgttct ccggaaatcg atctttatgc ggtggtttgt tcttgacgaa tccagtcgta
     5461 gcccttttct tccaactcaa gcgccagatt ggcatctccg gtacgaacga tttgaccgtc
     5521 catcatcaca tgaaccgcct gcggttttat gtaatccagc aatcgctgat aatgggtgat
     5581 aatcaagaca cccatatggg tctgatcgac caagatatta attccttcgg agacgattcg
     5641 taatgcatca atatccaaac ccgaatcgat ttcatccaaa atggcgattt ccggttcaag
     5701 aatagccatc tgaagaactt cggtccgttt cttttcccca cccgaaaatc cgtcgttcag
     5761 atatcgaccg gcaaaatccg gatccatttt cagcatcttc atcttttcct ttaggagttt
     5821 tcgaaattcg ggaatcggaa ttccgtgatc atccggattc ttgcttttac ggtgaatatt
     5881 taatgcggtc cgcagaaaat ttgccacggt caccccggga atcgctaccg gatattggag
     5941 tgccaaaaac aatccggcgt gtgagcgctt gtcggggggc caatcattga ttaattgatc
     6001 gttcaagaga atttcacctt cgtcgggtcg ataagcagga tgtcccataa tcgtatagga
     6061 gagggtagat tttccggttc cgtttggtcc cataatcgcg tgaatctccc ctttgggaac
     6121 ctgaatggac aggtgattga taatcttttt atcatgaacg gtgacgctta agtctttaac
     6181 ttctaagcct atcattaaat tctcctcaaa tgcaccgata ttggaatcgg ttgagtcatt
     6241 ttttcatctt ttattcaacg acagcgggga aagagttgat aaacccgcat tataaaatca
     6301 tcgattttaa taacggataa attataacag attgacgtct ttgaagtgaa agcttcgcct
     6361 caagcggttg aatttctaac ggaattctaa gaaaggggca aaccgatacg gctctcattt
     6421 caaatttata ctctttttga atcattattg tgaaatccgt tagaattaag ccgtatttat
     6481 cgttgaaatt tatcaaattc aaggaaaaag attaaggagg atccgatgag aatcattttt
     6541 ctgagacatg gtgaagcgat cgaacgaggc agtgtggatg atgaacaacg gttcctcaca
     6601 gagagaggta aaagagatct ggaaagcaat ctaccggcgc tcgccgctta tttacgcaac
     6661 cgaaataatg tggaaatctg gtcctctccg aaaatacgag cgattgaaac tgctcagatc
     6721 ttaacatccg gtatggagaa taaagatatc cggtttaaag attttatcgg ggacggagat
     6781 acggaacaac tgaaatccga attggagaaa accggagctg agacggttgt tattgtgggg
     6841 catcaacctt atttgtccga atgggtggat gaactttgcg attctgagat cctttttcaa
     6901 aaaggagcgg cggcggaaat agagtattat ccggacagtg accaaaaagg caaagtacta
     6961 tggcattttc cgttgaaagg tttgaagcag ttggctcgaa tgacggggcc ggaagttgaa
     7021 ggaaattctt ttcgacagga tctgatgata aaaatggagc aaaacttcaa tcagatcgca
     7081 gaaaatagga atcgttttct atccgaaccg gatgatgtcg aatcggcaca tcaactgaga
     7141 gtatctatcc gtagttttcg ttccttgata tctctcggaa aaaaatattt aaaaccggaa
     7201 aaatatcagg attatcaaga tcaatttcga caggcagctg ctgagataag cactctccgt
     7261 gaaattgatg tgatcatcaa tacttggaac gaacacttac tcgatcagaa actcgatccg
     7321 tctcaatctg tgttgatcca ggagttgaat cgaaaacgaa atatagaaaa agatcgggtt
     7381 ataaaaattg tatccaagaa taaatatatc gatcgcctga caaaaagttc taacgatctt
     7441 ataaagacca ttcgaaaaag cagcgaaaag gatgtcccga ctcaaaaagt gattgatgcc
     7501 cggcttgaca aatggtatca atatattttg aaacggatga ttaattataa cgaattcaac
     7561 ttttcatata ttcatccgat ccgtctgaaa tgtaaaaaat atcgttatgt cagcgaaaac
     7621 ttttatggca tactggatct ggaacatcga aaacgtcatc gggatgtaaa gcaactccag
     7681 acattattgg gaaatacctg tgatgcgatc cgtaaccaag aagcgattct gaaaatcatc
     7741 ggtttgagat ctccggcgtt ggagcaggaa gtgatcgctt ttatcaatta tgaaaaagag
     7801 attgaatcga attacaggaa attatttgac agtcgcgaat ggcttcaaca aattagcgaa
     7861 aaggaaaatt aaattaaatc ccggttttat acttttatgc acattgtccc ttatgggtat
     7921 ttttgatttc gtgaaaaggg ctgataaaat aggcaaaaag gaaaaagttc tctgtataca
     7981 gaaccggata aggaaggtat gaacgaaatt tttgaaaaat tttcgatgaa agatcaaacc
     8041 gcggtgataa cgggcggcgg aggattgctc ggcaaagaat tttgcaagac acttgcgcaa
     8101 gccggtgcaa ccatagtcgc ggcagattta aatctgaatt tagcccggca aacagtcgag
     8161 ttgattcaag aattcggagg gcatggaatt gcctgcgctg tggatgttac cgatcccgaa
     8221 tcggtgaaag cgatggtcga tttggctgtc aacgagaccg gacggttgga tgtgctggtt
     8281 tgcagcgcgg ctttggatcc gaaatttgac ggaacgcaag ggaaacattc gaatacattt
     8341 gaagaatacc ctttgaaaca atggcgggat gctttggatg tcaacctgac cgggcttttt
     8401 ctttgcgctc aagcgtcggt aaaaccgatg atcgcccaaa atcacggagt catcatcaat
     8461 atttgttcga cttacggatt ggtcgggccc gaccaacgga tctatgaaag gccggacggg
     8521 gatccgtcat cgaggcagta taaacctgtt tattattcgg ttacgaaagc cggagtgctc
     8581 ggattgacca aatacttggc cacttattat gccggcacgg gaattcgggc aaactgcctg
     8641 acaccgggcg gtgtctataa cggacacgac gaattttttg ttaagcaata ttcggcccga
     8701 accgttatgg gtcgaatggc aaatatcgac gaactcagcg gagctttgtt atttttggca
     8761 tccgatgctt cttcttatat gaccggatcg aatttggtgg tggatggcgg atggacggca
     8821 tggtagctaa gcacgcagaa gttttggcga tcattccggc caggggcgga tcgaaaggaa
     8881 ttccgcataa aaacattaaa aaatttgccg gatatccttt gattgcctgg tctatcgcag
     8941 ccgcaaaaca atccgcatcg gttacacgga caatcgtctc tacagacgat gaggaaatcg
     9001 cacgggttgc gcgcgaatgg ggagcagaaa cgcctttttt acggccggcg gagtttgcaa
     9061 tggatcaatc attggatttg ccggtttttc ggcatgcgct ggaatggtta aaagaaaatg
     9121 aggggtatga acccgatatc gttgttcagc tgagacctac ttcgccggtt cggccggtga
     9181 atttagtgga tgatgccgtc cggctgctca cggatcatcc ggaggccgat tcagttcgcg
     9241 gcgtagtgcc ttcctctgag aaccctttca aaatgtggct gatcgattct gaaaccggaa
     9301 cgatgagcgg attgatcaaa ttagatggca ttccggaacc ctataatgca ccgaggcagg
     9361 tattacccgc aacatattgg caaaccggac atatcgatgc gatacgtccc cggcggactt
     9421 ttatggcggg cgacagtatg agcggaaaag tgattttgcc tcttttattg gatcccgctt
     9481 ttaaagtgga tatcgatact ttggcggact gggaccgtta tgaacaactg gtctattacg
     9541 gcggtttgga tatggtttat cccgaacaca agagaagatc actgccgcaa aaaacgtgtt
     9601 tgttggtcat ggacttcgac ggtgttctga ccaatgatga ggtcatcgtg aaccaggatg
     9661 gacttgaaag tgtgatctgt aatcgcgggg acgggatcgg tttttggctg ttgcgggaag
     9721 ccggaataac cgcgattgtg atttcaagcg agaccaatcc ggtggtgcgg aaacgctgcg
     9781 aaaaattggg aatacaatct tatcagggga cgttagataa agcgactgtg ttgaagaaat
     9841 atttggagga gaataaaatc ccggcggaaa atactgttta tatcgggaat gatatcaacg
     9901 atttacaatg ttttccgttg gtaggttgtg cggctgctcc tgcagatgct catgatcttg
     9961 taaaatcaaa agcagatata attctcaaac gaaacggcgg acaaggtgtc gttcgtgagt
    10021 tatgcgaatt gattgtccgg cgaaataagg aaagttatgg gaaagacagt taaattagga
    10081 aatcggttag tcggcgaagg ccaaccggtc tatgtcattg cggaaatcgg aatcaatcat
    10141 aacggagata tcggcattgc gaaacgcatg attcgctcgg cttatgcggc cggagtggat
    10201 gcggtcaaat ttcagaaacg gactcccgct gtttgcgttc ccctttcgga acaaagcaaa
    10261 atgcgtgaga ctccttgggg ttacatgtcc tatcttgagt atcgggaaca tgtcgaattt
    10321 tgggaagatg aatatcatga aattgatgaa ctgtgccatg agttgggaat cgattggttc
    10381 gcttcggtat gggatgtgga atcaattgat tttttgatga aatttgatcc cattgcctac
    10441 aaagtccctt ccgcaagtct gaccgatcat gaattattgg ataaactgct gtcaacccga
    10501 aagccgatta ttctgtcgac cggcatgtct acgatggatc aaatccg
//
LOCUS       DAOMIV010000010         9684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_7092_length_9684_cov_3.843390, whole genome shotgun sequence.
ACCESSION   DAOMIV010000010 DAOMIV010000000
VERSION     DAOMIV010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7092_length_9684_cov_3.843390"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..382
                     /gene="rpsJ"
                     /locus_tag="PKV59_00525"
     CDS             74..382
                     /gene="rpsJ"
                     /locus_tag="PKV59_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQP45262.1"
                     /translation="MAKQKIRIRLKAFDHRILDQSAKRIVDTAERSGAHVVGPVPLPT
                     RIERFTVRRSTFIDKDSQEHFEIRTHNRMIDVLDPDSKTIDMLMRLNLPAGVDIEIKM
                     "
     gene            complement(445..864)
                     /locus_tag="PKV59_00530"
     CDS             complement(445..864)
                     /locus_tag="PKV59_00530"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQP45263.1"
                     /translation="MPTRKSQAIWEGSLKEGKGRMMLGSGAFEGKYSFGSRFEEEPGT
                     NPEELIAAAHAGCFTMFLSSLLTDAGFTPRKITTTAKVNLAAGPEISKIELETEADVP
                     GISDKVFQEQALIAKKNCPISKALSAVPEITLSAKLV"
     gene            1043..>2106
                     /locus_tag="PKV59_00535"
     CDS             1043..>2106
                     /locus_tag="PKV59_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQP45264.1"
                     /translation="MNLTRKQWLSLIFIGLTGQLAWMIENMYLNLFMYQTASTDPHYI
                     ALMVSASAVVATLTTLLMGNLSDRFGKRKVFISAGYILWGISIILFSFVNRENIQALY
                     PFANASVVAAYGIVVLDCIMTFFGSTANDAAFNAYVTDITVPTNRGKVESVLATFPLI
                     AMLIVFGCFDPIVQQGNWNLFFRIAGGIVLAVGFLSLFFIKEVPVEKKKSNFFAELFY
                     GFTPASFHENKNLYIIMIAFCIFGAAFQVFFPYLIIYIQQFLFIENYALILGTILIIA
                     SLISILMGPVIDKYGKMEVIYPAIITMLIGLVGLFFSRRNIPVTFWGIIMMSGYMMIS
                     ATLSALIRDFTPPGKVGVFQG"
     assembly_gap    2107..2206
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2207..2481
                     /locus_tag="PKV59_00540"
     CDS             <2207..2481
                     /locus_tag="PKV59_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45265.1"
                     /translation="SALIRDFTPPGKVGVFQGVRMIFTVMLPMVIGPYIGAWLIKSNG
                     GIYEELGQTKNIPTPDIFLVSAFVLCFIVIPLYFLGKNQHENKMGA"
     gene            2459..4171
                     /locus_tag="PKV59_00545"
     CDS             2459..4171
                     /locus_tag="PKV59_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HQP45266.1"
                     /translation="MKTKWGHNLNPDAILSEYPRPQMERDHYLILNGFWNYAITESSE
                     QPAQFDGDILVPFSPESELSGVNRILTKDQYLWYRREIVLPDDFLLDHLLLHFGAVDQ
                     KATVYLNGIEVGSHLGGFTPFSLDITMAWQKNDRNILTVKVQDTTDFSWNTRGKQRWK
                     PGGIWYSPQSGIWQTVWLESVPSDYIQSIQITPDFDAACVRFFICSERNLSCLLEISG
                     SEFRFPSNELVTVPMPNFIPWTPDQPYLYSFTAQLADDKVNGYFGMRKFSVGSDSDGT
                     KRFFLNNQPIFLSGLLDQGYYSDGLLTAPSDEAMIYDIQTAKSMGFNLLRKHVKIEPL
                     RWYYHCDRLGMIVWQDMPNGGKAYKDWLISAPVVVNFRINDRHYKWLGRKEKASREQY
                     LVELEEMIRHLYNVVSIGMWVPFNEGWGQFDAVEVTKQIKKTDPSRTIDSASGWFDQF
                     TGDFLSRHVYFKKYRYKKDKLNRAVILSEFGGYSYRVEGHVYSENNFGYKQFKSEEEF
                     QKAFISLYENEVIPFKVKGLTAAVYTQLSDVEDELNGLLSYDREVIKISPEIVQEINQ
                     RLLS"
     gene            4380..5543
                     /locus_tag="PKV59_00550"
     CDS             4380..5543
                     /locus_tag="PKV59_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45267.1"
                     /translation="MKKFFSLVILLIVVISAVGFAPVTQDENVVTFGVFEPLTGENGG
                     GGYQEVLGIRYANKVYPTVEAGGKTYTIKLYEVDNKSDKTEAVTAAQNIVSKNVNAII
                     GSYGSGVSIAAGAIFEEAQIPAMGASCTNPQVTYGNDFYFRVAFLDPFQGTVMANYAF
                     QNGAKTAAVITQLGDDYSSGLGKFFVDAFKKLAGDAAIVAEEQFQTNQTDFKAILGNI
                     KAKNPDVIFAPSSIVTAPLIIKQAREMGITAKIMAGDTWENATIIENAGKDAEGVVLS
                     TFFDEADPATEEAKKFVEGFKPFLVENNQPDIIPAVSALGYDAYLALYKAIEAADSVD
                     PIAIRDALVTLEFDGVTGHIKFDENGDAIKNMAVIKVIQDGAFKFLQTVTVSE"
     gene            5750..6631
                     /locus_tag="PKV59_00555"
     CDS             5750..6631
                     /locus_tag="PKV59_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQP45268.1"
                     /translation="MSLTTILQHSLTGISLGGAYALIAVGYTMVYGILRLINFAHGEI
                     FMMAGYFMIFSMASFPWYISIPIVLAGTALLGVSIEKVAYKPLRSAPRMSVMISAIGV
                     SYLLQNLATYLFTALPRGYPEIPGLKKVFQIGDLSSSVVTFVTPVVTILLAIVLVQMI
                     NHTKIGMAMRAVSKDLDTAQLMGIQINSVISFTFLIGSLLAAIGSILYFTDRMSVNPF
                     SGSLPGLKCFVAAVLGGIGSIPGAIVGGFILGIGETFMVAAGFSTFSDAFTFIMLIFM
                     LLVKPTGLFGEKEPEKV"
     gene            6631..7725
                     /locus_tag="PKV59_00560"
     CDS             6631..7725
                     /locus_tag="PKV59_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQP45269.1"
                     /translation="MTLKKSTKTILSITVLVLIALLLFHLEQDKRANSYILSILERSI
                     IYAVVAVSMNLLNGFTGLFSLGQAGFMALGAYTVGILTIPVENRPSVYYTSGINETIA
                     NWHLPFLPALLMGGLVAALAAALVGALILKLKSDYLAIATLGFSEIVRAVIASPQLDM
                     ITNGSYGLKKIPRFTTVYIPFALAAACILLMILLINSSYGRAFKAIREDEVAAEAMGI
                     NLFKHKELSFVISSFFTGIGGGMLACYMGSIDSGTFRITLTYDMLLIVVLGGLGSVTG
                     SILGSFLVTAGREWLRFFDEPHAIFGWQIPLFRSGFRMVVFSVLLMVVVLFYRKGIMG
                     DKEFSWESFFGFFKRLIPKKKKLAGGNTHD"
     gene            7718..8566
                     /locus_tag="PKV59_00565"
     CDS             7718..8566
                     /locus_tag="PKV59_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45270.1"
                     /translation="MTESVLRIDHCTMQFGGVVAVDDLCMEINKDEIVALIGPNGAGK
                     TTAFNFVTGVYEPTNGRVYFNDKLLVANHPQGKMKKLYMGEFEKICPDVVRKTPDQIT
                     RLGIARTFQNIRLFKNLTVLENVLIAMHLHMKSGLIAAATHLNFKEENQMRKEALELL
                     EMQNLIHVKDEIASSLPYGQQRHLEIARALATKPTLLLLDEPAAGMNPQETDELAEFI
                     RKTKEDFKLTVFLIEHHMTLVMDISDRIYVLDFGKMIAHGSPDAVQNDERVIHAYLGV
                     ADAEDQ"
     gene            8550..9272
                     /locus_tag="PKV59_00570"
     CDS             8550..9272
                     /locus_tag="PKV59_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45271.1"
                     /translation="MLKINNLKVSYGGIKALNGISLEIPDGKIVTLIGANGAGKSTTF
                     KAIVGLVKPVSGEIWFKDQNITGLPTNKIIGSGISTVPEGRKVFSDMSVLENLRIGAY
                     LRKDEEGIRRDIDMVFDLFPRLKERHWQQAGTLSGGEQQMLAVGRGLMGHPQLMLMDE
                     PSLGLAPLIVQEIFSIIKKINDEGVTILLIEQNANMALRTADYAYVMETGHITMQGTG
                     RELLAEESIRSAYLGKRRKAKV"
     gene            complement(9360..>9684)
                     /locus_tag="PKV59_00575"
     CDS             complement(9360..>9684)
                     /locus_tag="PKV59_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQP45272.1"
                     /translation="QSFINAHYASHLSLDEISYEMAISKSKLCKIAREIDKNMTVGKL
                     ITQKRIEAAKKKLIESDMPIREISEIVGIPDFNYFTKVFKRATGSTPSEYRSHFSEQA
                     VHPEE"
BASE COUNT         2573 a         1828 c         2236 g         2947 t
ORIGIN      
        1 tcgcgaaggc gggttgaccg tcggcgccgg tgtgattact gaaatcgaag gataacggga
       61 agaaagcaag aaaatggcaa agcaaaagat tcgtattcgt ctgaaggcgt ttgatcaccg
      121 gattttggat caatccgcaa aacgcattgt tgataccgcc gaacgcagtg gcgctcatgt
      181 cgtaggcccc gtaccgcttc cgacccgaat tgaacggttt accgttcgcc gatcaacttt
      241 tatcgataag gattctcagg aacattttga aattcggact cacaatcgaa tgatcgatgt
      301 gttggatccg gattcaaaaa ctatcgacat gctgatgagg ttgaatctcc cggcgggtgt
      361 ggatatcgaa atcaaaatgt agtctgtcgt aaaaaatcaa aaaagccgga agcctccggc
      421 ttttttgatt attccttatc aggattaaac taattttgca cttaatgtta tttcgggaac
      481 cgccgacaac gcttttgaga tcggacaatt tttctttgcg atcagagctt gttcctgaaa
      541 cactttatcg ctgattccgg ggacatccgc ttccgtctcc aattctattt tcgaaatctc
      601 cggaccggca gcgagattga ctttggcagt tgtagtaatc ttgcgcggtg tgaatccggc
      661 atcggttaaa agggaagata aaaacatcgt aaagcagccg gcatgtgcag ccgcaatcaa
      721 ttcttcggga ttggtgccgg gttcttcttc gaaccgtgat ccgaatgaat attttccttc
      781 aaaagcaccg cttccgagca tcatcctacc ttttccctct tttagtgaac cttcccatat
      841 tgcttgtgat tttctggtgg gcatttttat tctcctttta atttagatca aaaccttaat
      901 ttttatttta tcccgattcg ttcaaatatc ggatatttcc ggatgaagaa gatccgtaaa
      961 actctgtctt taaatcaagt agaatagaat tgcaatgttt aatagtctgc ttgaaatctg
     1021 aatggacagg aataatgaaa gaatgaatct aacacgaaaa caatggcttt ctctgatttt
     1081 tatcgggttg accggacaac ttgcatggat gatcgagaat atgtatttga atttattcat
     1141 gtatcaaacc gcctcaaccg atcctcatta tatagcgttg atggtttctg ccagtgcggt
     1201 tgtcgcaact ttaacgactc tattgatggg aaatttatcc gatcgattcg gcaagcggaa
     1261 agtatttata tcggcggggt atattttatg gggaatttca attattctgt ttagttttgt
     1321 aaaccgagaa aatattcagg cactgtaccc gtttgctaac gcttccgttg tcgccgcata
     1381 tggaatcgtt gttctggatt gtataatgac ttttttcggt tccacagcca atgacgcggc
     1441 gtttaacgct tatgtcacgg atattactgt tccgaccaat cgcggcaaag ttgaatctgt
     1501 attagccact ttccccttga tcgctatgtt aatcgtattt ggctgctttg atccgatcgt
     1561 acaacagggt aactggaatc tttttttcag aatagccggc gggattgtat tggcggtggg
     1621 attcctttct ctgtttttca ttaaagaagt gccggttgaa aagaaaaaga gtaatttttt
     1681 tgcggaattg ttctatggat ttactcccgc ttcttttcat gagaataaaa atttgtatat
     1741 tatcatgatt gcattttgta tattcggagc cgctttccaa gtattttttc cgtatttgat
     1801 tatttacatt caacaattct tatttataga gaattatgca ttgattttag gaacgatttt
     1861 gattatcgct tctttgatca gcattttaat gggcccggtg atcgataaat acggcaaaat
     1921 ggaagtcatt tatccggcga ttatcacaat gctgatcggt ttggtcgggc tatttttctc
     1981 gcgccgaaac attccggtga ctttttgggg gatcattatg atgtcgggat atatgatgat
     2041 tagcgcaact ctttccgcgc ttattcgaga ttttaccccg cccgggaagg taggtgtttt
     2101 ccaaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntttc cgcgcttatt
     2221 cgagatttta ccccgcccgg gaaggtaggt gttttccaag gcgttcggat gatttttacc
     2281 gttatgctgc cgatggttat cggaccgtac atcggagcat ggcttattaa atctaacggg
     2341 ggaatctatg aagaactcgg gcagacaaag aatattccta ctccggatat ttttcttgtt
     2401 tctgctttcg tgttgtgttt tatcgtaatt ccactctatt tcctcgggaa aaaccaacat
     2461 gaaaacaaaa tgggggcata acctaaatcc ggatgccatt ctgagcgaat acccgcggcc
     2521 gcagatggaa cgggatcatt atttaatact gaacggtttt tggaactatg cgataacaga
     2581 atcatccgag caaccggctc agtttgacgg tgatattctt gttccgtttt ctcccgaatc
     2641 ggaactgagc ggcgtcaatc gcatattgac gaaagatcaa tatttatggt atcgaagaga
     2701 aatcgtcctt cccgatgatt ttttacttga tcacctgctg ctgcatttcg gagcggttga
     2761 tcaaaaagcg acggtatatc ttaacggaat tgaagtcggt tctcatttag gcggatttac
     2821 gcctttttca ctcgatataa ccatggcatg gcagaaaaat gacagaaata tactaacggt
     2881 aaaagtacaa gacactacag atttttcatg gaatactcgc gggaaacagc gttggaagcc
     2941 cggcggaatt tggtacagcc cgcagtccgg gatttggcaa acggtatggt tggaaagcgt
     3001 tccgtccgat tatattcaat ccatacaaat tacccctgat ttcgatgccg cctgtgttag
     3061 gtttttcatt tgttccgaac gaaacttatc ttgtctcctt gaaatcagcg ggagcgaatt
     3121 ccgattccca tcgaatgaac tggtgaccgt accgatgccg aattttattc cctggactcc
     3181 cgatcaacct tacttatatt cgtttacggc tcaattggcc gatgataaag tcaacggtta
     3241 tttcggaatg cgaaaatttt cagtcggttc ggattccgat gggacgaaac gatttttctt
     3301 gaataatcaa ccgatatttt tatccggttt attggatcag ggatattata gtgatggatt
     3361 gttaacagct ccgtcggatg aagcaatgat ttatgatatc caaaccgcaa aatcaatggg
     3421 attcaatcta ttgagaaagc atgtaaagat tgagcctttg cgttggtatt accattgtga
     3481 tcgtctcggt atgatcgttt ggcaggatat gcctaacgga gggaaagctt ataaagattg
     3541 gttaatttcc gctccggtag tggtgaattt tcgtatcaat gaccgtcatt ataaatggtt
     3601 gggaagaaaa gaaaaagcgt cgcgggaaca atatcttgtc gaactggagg aaatgattcg
     3661 gcatctgtac aatgtcgttt caatcggaat gtgggttcct ttcaacgaag ggtggggaca
     3721 atttgatgct gttgaagtga caaagcagat aaaaaaaacg gatccttcgc gaacaatcga
     3781 ttcggccagc ggctggtttg atcaatttac cggagatttt ttaagcagac atgtttactt
     3841 taagaaatat cgatataaaa aagataaatt gaacagagcg gttattttgt cggaattcgg
     3901 cggatacagt tatcgtgtgg aagggcacgt ttacagtgaa aataatttcg gctacaaaca
     3961 gtttaaaagc gaagaagaat ttcaaaaagc ttttatttcg ctctatgaaa acgaggttat
     4021 cccttttaaa gtgaagggat tgactgccgc ggtttataca caactttccg atgtcgaaga
     4081 tgaattgaac ggattattat cttatgatcg tgaggtgatc aagatttccc ccgaaattgt
     4141 tcaggaaatc aatcaaagat tattatccta aatttataat ttccatagag aatataaatt
     4201 taataagtcg aattagaaag aatgattgtt ttaatccatc aactttgatt tcgcaatgat
     4261 gatattatca aattttattg ctaacaattt ctttgcgtgc tataatgcaa aaatacattt
     4321 ataagtctga aagcgtttgg aaaaccggat tattccggaa tcaatctatc ggaggagcta
     4381 tgaaaaaatt tttttcgttg gtaattctgt taattgtcgt aatttcggca gtcggttttg
     4441 ctcctgtaac acaggatgaa aatgtggtta catttggagt atttgaacct ctgaccggtg
     4501 aaaatggcgg gggcggatac caggaagttt tgggaattcg ctatgccaat aaggtttatc
     4561 cgaccgttga agccggcgga aaaacttata cgatcaagct ctacgaggtg gataataaga
     4621 gtgataaaac cgaagccgta accgccgcac agaatatcgt cagcaaaaat gtgaatgcga
     4681 ttatcggttc ttatggttcg ggcgtatcta ttgccgcagg cgcgattttt gaggaagctc
     4741 agattccggc gatgggagcc tcttgtacca acccgcaggt gacttatggg aatgatttct
     4801 atttccgagt tgcttttctt gatccgttcc aagggacagt aatggccaat tacgcattcc
     4861 agaatggcgc aaaaactgcg gcagtgatta ctcaacttgg cgatgattat tcgtctggat
     4921 tggggaagtt ctttgttgac gcgttcaaaa aattagccgg tgatgctgct atcgtcgccg
     4981 aggaacagtt ccagacgaat cagacggatt tcaaagctat tctcgggaac attaaagcca
     5041 agaatccgga tgttattttt gctccttctt ccattgtaac cgccccgtta atcataaagc
     5101 aggcgcgtga aatgggcatt acggcaaaaa ttatggcggg agacacctgg gaaaatgcga
     5161 caattattga aaatgccggt aaagatgcgg aaggtgttgt tttatctacg ttctttgatg
     5221 aagccgatcc ggctaccgaa gaagcaaaga aatttgtgga agggtttaaa cctttcttgg
     5281 tagagaataa tcaaccggac attattccgg cggtttcagc tttaggctat gacgcttatc
     5341 tggctttata taaagccatt gaagctgccg attccgttga tccgatagcg attcgggatg
     5401 cgcttgtcac tcttgaattt gacggtgtaa ccggtcacat taaattcgac gaaaatgggg
     5461 atgccattaa aaatatggcg gttattaaag tgatccaaga tggagctttt aaattcttac
     5521 agactgtgac tgtttctgaa taaatggaca gcatgaaaag aatgttgaag ttatttcttc
     5581 acgttatttt ccttgaacga aggtagttct gttttgttgc ttcggatgaa aggtgcctta
     5641 ggcacctttc atcttttcgg tctgaattag atttgtgatt ttttgatcaa atatttcagc
     5701 ccgttttctg ctgtgagatt ttacggaaat aaaggagggg caaccttcta tgagtttgac
     5761 gacaatattg caacatagcc tgacaggaat ttcattaggc ggtgcatatg cactgattgc
     5821 ggtgggctat accatggttt acggaattct gcgcctgatt aattttgctc acggtgaaat
     5881 ttttatgatg gcaggctatt tcatgatttt ctctatggct tcatttcctt ggtatatttc
     5941 catcccgatt gttttagccg ggacggcttt attgggggtt tccattgaaa aagtagctta
     6001 taaaccgctt cggtccgcac cgcggatgtc cgttatgatt tctgcgattg gcgtttccta
     6061 tcttttacaa aacctcgcaa cctatttatt tacggctttg ccgcgaggat atccggaaat
     6121 acccggactg aagaaagttt tccaaatcgg agatctttct tcttcggtcg taaccttcgt
     6181 aaccccggtt gtcacgatcc tgctggctat tgttctggtt cagatgatca atcacacaaa
     6241 aatcggtatg gcaatgagag ccgtatcgaa agatctggat actgcgcagt tgatgggaat
     6301 tcaaattaat tccgtaatca gcttcacatt tttgatcgga tcgctgttgg cggcaatcgg
     6361 ttccatcctt tattttaccg acaggatgtc ggtaaatcct ttcagcggtt cccttccggg
     6421 actaaagtgt ttcgtcgcgg cagttctggg cggcatcggc agtattccgg gtgcgatagt
     6481 gggcggcttt atactgggga ttggtgaaac ctttatggtt gccgcgggct tttccacttt
     6541 cagtgatgcc tttacattta tcatgctcat tttcatgctt ttggtaaaac cgacggggtt
     6601 gttcggtgaa aaagaaccgg agaaagtctg atgacactca aaaaatcaac caaaactatt
     6661 ttatcgatca cggttctggt cctgattgcc ctgcttttgt ttcatctgga acaggataaa
     6721 agagccaaca gttatattct ttccatattg gaacgcagta tcatctatgc ggtagtggct
     6781 gtatcgatga acctgctgaa cggtttcacc ggattatttt ctctcggaca agccggattt
     6841 atggcgctcg gagcttatac cgtcggtata ttgaccattc cggtcgaaaa ccggccgtcg
     6901 gtatactata ccagcggaat aaatgaaacg attgccaatt ggcatctgcc ttttttgccg
     6961 gctttattga tgggagggtt ggtggcagct ttggcggctg cgctggtcgg tgccttaatc
     7021 ttgaaactaa agagcgatta tttggctatt gccactctcg gtttttcgga aatcgttcgg
     7081 gctgtaattg ccagtccgca actggatatg attaccaacg ggtcgtatgg attaaaaaag
     7141 atcccccgtt ttacaacggt atatataccg tttgctctag cggctgcctg tattttactg
     7201 atgattttgc tcattaattc ttcctacgga agggcattta aagccattcg agaagatgaa
     7261 gtcgccgccg aagcgatggg gattaattta ttcaagcata aagagctttc ttttgtgatc
     7321 agctcattct tcaccggaat cggcggagga atgttagcct gttatatggg atcgatcgat
     7381 tcaggcactt tcagaatcac gttgacttat gatatgttgc tgattgttgt tttaggcggg
     7441 ttgggcagcg ttaccggatc catattggga tcattcttgg ttacagccgg tcgggaatgg
     7501 ttgcgtttct tcgacgagcc gcatgctatt tttggatggc agattccttt attccgttcc
     7561 ggattccgca tggttgtctt ttctgttctg ctgatggtgg ttgtgttgtt ttatcgaaaa
     7621 ggaatcatgg gtgacaaaga attcagttgg gagagtttct ttggattttt caaacgatta
     7681 atcccgaaaa agaagaaact agcgggagga aatacgcatg actgaatccg tcttgagaat
     7741 tgatcattgc acgatgcaat ttggcggtgt agtggctgtc gatgatttat gcatggaaat
     7801 taataaagat gaaattgtgg ctttgatcgg cccgaacgga gccgggaaaa caaccgcttt
     7861 taattttgtt acgggtgttt acgagccgac gaacggcaga gtttatttta atgacaaatt
     7921 attggttgcc aatcatccgc aaggaaaaat gaagaaactt tatatgggcg agttcgaaaa
     7981 aatttgcccg gatgtggttc gaaagacacc tgatcaaatt acccgacttg ggattgcccg
     8041 gacatttcag aatatccgat tgtttaagaa tttgaccgtg cttgaaaacg tgttgattgc
     8101 catgcatttg catatgaaat cgggattaat cgctgccgca acccatttga attttaaaga
     8161 agaaaaccag atgcgcaaag aagcgttgga attgctggaa atgcagaatc tgattcatgt
     8221 aaaagatgaa atagcaagct ctctccccta cggacagcag cgtcatctgg agatcgccag
     8281 agcgttggcc actaaaccga ctctactttt attggatgaa ccggctgccg gaatgaatcc
     8341 tcaggagacg gatgaacttg ccgaatttat tcgaaaaact aaagaggact tcaaattaac
     8401 ggttttcttg attgagcatc atatgaccct tgttatggac atttccgatc ggatttatgt
     8461 gctggatttc gggaaaatga ttgcccacgg ttctcccgat gctgttcaga atgatgaaag
     8521 agtcattcat gcttatttgg gggtggcaga tgctgaagat caataatttg aaagtttcct
     8581 acggcggcat taaagcttta aacggtattt ctctcgaaat tccagacgga aagattgtta
     8641 cgttgattgg tgcaaatggt gctggcaaaa gcactacttt taaagcgatt gtcggattgg
     8701 tcaagccggt ttcaggggag atctggttta aagatcaaaa tataaccggt ctgccgacaa
     8761 ataaaattat tgggagcggt atttcgacag taccggaagg gcgaaaagta ttttccgata
     8821 tgagtgtcct tgagaatttg cggatcggtg cgtatttgag aaaagatgaa gaaggaattc
     8881 gcagagatat cgacatggta ttcgatctct tccctcgttt gaaagaacgc cattggcagc
     8941 aagcggggac cctttcgggt ggtgaacaac aaatgttggc ggtcggacgc ggactaatgg
     9001 gtcatcctca gctgatgttg atggatgaac cttctctcgg cctggcgcct ttaattgttc
     9061 aggagatttt ctcaatcatt aaaaaaatca atgatgaggg tgttacgatt ttattgattg
     9121 agcagaacgc caatatggct ttgagaacgg ccgattacgc ttatgtaatg gagaccggcc
     9181 acatcactat gcagggtaca ggaagggaat tgcttgccga agaaagtatt cgatcggctt
     9241 atttgggaaa acgaagaaaa gcaaaagttt aatttgattt tttatcgtga ggatcgggat
     9301 cggtttacaa ggaacgatcc cgatcttttt atttcagtaa aaatccgaga gacgatcaat
     9361 cattcctccg gatgaaccgc ttgttcagaa aaatgactgc gatattcact cggggtggaa
     9421 ccggtcgctc gtttgaaaac ttttgtgaaa taattaaaat ccggaattcc gactatttca
     9481 gaaatttctc ggatcggcat atcggattcg attagtttct tttttgccgc ttcaatccgt
     9541 ttttgagtga tcaactttcc gacagtcata tttttatcga tctctctcgc tattttacaa
     9601 agtttgctct ttgatattgc catttcataa gatatttcat ccagcgacaa atgagatgcg
     9661 taatgagcat taataaaaga ttga
//
LOCUS       DAOMIV010000011         9408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_7380_length_9408_cov_4.542286, whole genome shotgun sequence.
ACCESSION   DAOMIV010000011 DAOMIV010000000
VERSION     DAOMIV010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9408
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7380_length_9408_cov_4.542286"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PKV59_00580"
     CDS             complement(<1..1292)
                     /locus_tag="PKV59_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45273.1"
                     /translation="MKHSLRFLIPSGLISAAATLGLYQMLPLMHFNTKLTALLIGFFL
                     AGTFFLFFFIRFLAKKISINHKMIGIFIVASVFLSVLITFLFHLSFPQKEIVLPNRKI
                     HIDVFPDQALADKTKIQFLSLYNGYRGISLSDFSTYGDWKRENDQLVLENFQNGDALE
                     FKGKAGRNIHLYFMVGPRSGKIRIDWGDGSSESYDLSSPMNEEDSLRISHDYGPSAGR
                     FELFNFLINLLSVVSFIFALVMLYLVLVYAFVRKRTKAFKTAFIIISLSTVLIRVVSV
                     YTFPLGWDEGTYSRAAMRYADKALSFQWKEIPSITYNHEHPALVKLTFAIPVILDGRP
                     YYQRFGLNTRNNAMLAKEDYTIFTGRIVSAVFSLWTVQVLAVMIHPFAAFFFMIHSLA
                     EEFGAQARLEAMPMLFSFLSIWFFSQFLKGMELRQKKG"
     gene            1423..2898
                     /locus_tag="PKV59_00585"
     CDS             1423..2898
                     /locus_tag="PKV59_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45274.1"
                     /translation="MPNPNQPQPDPKQPNHNQGKPNPSGYPYPPAGNPGNWPWQPNDR
                     VSGYPPNRQYSNPQKQNSTNPSLNNNPKKKPGPKKDRMDEFGRIFTMILSAFILIYMG
                     WLFAPRIRNSMTFWPTKTPTPVTPSPTLISTPTITPLASNTPTLMPTETPGPLSEYWI
                     ADGESIDPAVPDSPDGVIVLHADKNAEVNPPLDDVTWTSSEKIASDLGKSTYTENWFA
                     TYSSGWIRWFMDQPLREGLYEIYTMDTMYSSGGSLDFAVSLGDQLLTPLTGSQHVDFM
                     TSQYDPVQSADTWRSIGIYYVGPSTEALNVTTSWEDRDEYTIVAVDRLLIVPRRVSDL
                     GMLNSLSVSGTKYVWDDLEAEITGKDYIISQKEQLSWDDSYQLVLDPKTDVEITFPNR
                     EPYPIGNYKLYAWMPASKGGITATVKFYLDNTVLPSDTGEESVSYTAPKADAGSWIEI
                     GSWTTDKYYERPRKIKLTIVIPEGSKGEFPVDCFALIHNGF"
     gene            2938..5037
                     /locus_tag="PKV59_00590"
     CDS             2938..5037
                     /locus_tag="PKV59_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HQP45275.1"
                     /translation="MNFVENGIYLNFDIAADGTLKLYHFGSRPFDPNTILDESQKTGF
                     RVVEVQMSGIDRPEERHGTKFIVTAPGYRLKYQRHSDTRNSFGRKLEFVLIDHETGTV
                     VTCHYQFFDGISVVRCWTEVENRGTENQTLEYVSSFALTGIGKEGLKSRDEKLTLWIP
                     HNSWQREIQWTKHKLPELGLCLNQPEKISRSSKTIGVMNSGNWSTKEYIPMGFLENTE
                     TGDSLIWQIEHNGSWRWEISDQNGHLYLQLSGPNETWSHWSKVLKPGDKFTSVPVAVG
                     TVTGDFEKAIAEMTSYRRVIRRPNEDNEKLAIIFNDYMHCLWSNPTTEKEIPLIDTAA
                     EMGCEYYCIDAGWYSPGFWWDSVGEWQVSLERFPGGLTEVMDYIRSKGMIPGIWLEIE
                     VMGIRCRLAEQVPDDWFFVRHGKRVYDRSRFQLDFRNPEVRAFADEVIDRFVNEYHVG
                     YIKMDYNIEPGIGTEVDADSFGDGLLGHERAYLQWIDDIFERYPDLIIENCSSGGLRM
                     DYAMLSRHSIQSTSDMDNDRMVATISANAPSVLTPEQAAVWSFPLAGDDREEVIFNMV
                     NAIMLRVHQSGQITQISPDRKALVKEALDTYKRIRPDIRRAFPFWPLGLSKYSDPWVC
                     MGLRTDRRAYIALWRRNSEQETISLPVSFLAGRSVKANILYPSCDPCPYCWNDGTATL
                     SVTLEKPYSARIFCLEF"
     gene            5464..6111
                     /locus_tag="PKV59_00595"
     CDS             5464..6111
                     /locus_tag="PKV59_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HQP45276.1"
                     /translation="MDGRVITKLSIQKRNPGRVNVYLDGKFAFGIPKDAAAGLKTGQE
                     LTESEIEKLQERDQEDRALSSALRSLNRRLQSESELQKKLIQKGFSPDQIESVMERLK
                     ENHLIDDNSFSAMWVENQTAFRPRSKRMLTLELKRKGVSEENIRESLKDLDEEKAALD
                     CGLRYVKRLQGLDRPNFQKKLSAYLARRGFGYELIDQTVHTLWADIQNSEKDSIL"
     gene            6128..7744
                     /gene="rny"
                     /locus_tag="PKV59_00600"
     CDS             6128..7744
                     /gene="rny"
                     /locus_tag="PKV59_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQP45277.1"
                     /translation="MYGNFYPGLGDNGFGFLSVSAQTRPPETGSNLLIIILAVVIGLL
                     LVVTATFFITRYLNTKFMKSQQLDAENIILVSRQKAQEIEEEARENANQIAKDAETEI
                     KRRRGELTREEDRLQKRRAEIDARIEKLEQREANLNKRQSNLDKRSNEIDKMAAKQLE
                     ELQKIAQMTTEEARQELLNMVEKDSRNDMARIIRQIEQEARMDGEKKAREIIATAIQR
                     IASDQVSEVTTSIVNLPGEEMKGRIVGRNGRNIRAFEQAAGVDVIVDDTPEAVMISCF
                     DPIRREVARRALDRLILDGRIHPANIEKTLNEEEKEVQKLIIEAGEQAAFEAGVVGLH
                     PEIIKMLGRLKYRTSYGQNQLSHSVEVAKLSSVIASELGANVELARLGALLHDLGKAM
                     DHNTEGTHAKIGAEFAKRYGVNPKAINIIESHHHEVDQESVEAVIVEAADAISGARPG
                     ARREDLELYIKRLRALEEIANSHKGVQNSYAIQAGREIRIIVTPEEVDDLEATYLARD
                     IAHQVEETMQYPGQIKVTVIRETRAVDYAK"
     gene            complement(7832..9217)
                     /gene="argH"
                     /locus_tag="PKV59_00605"
     CDS             complement(7832..9217)
                     /gene="argH"
                     /locus_tag="PKV59_00605"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQP45278.1"
                     /translation="MKEQTLWSGGRLDSEMDREMIQLNNSLWIDQRMAKEDVQGSLAW
                     ANAIFEAGVLSRSEYDSIISGLKQISEEFSNDSFQFKSSDEDIHTAVERRLTEIIGPA
                     GGKIHTGRSRNDQVVTDFRLWIKNHISEMKSGIETLQNAFLDRAEQDMGVILPGYTHF
                     QQAQPILLSHWWLSHFWALERDKQRLTEAQSHADISPLGCGALAGSTLPIDRFKLAKD
                     LGFSEPAPNSLDAVSDRDFVCDFLYEMAMIAVHMSRCAEALILYSTTEFGFITLSDRY
                     STGSSLMPQKKNPDSLELIRGKCGSQIGLLQAMLSTLKGLPSAYDKDLQEDKVLFFQS
                     WDNTLLLMKVLTGVVDTLHVNADRCRQAVDTQAFATDVADYLVKKGVPFREAHHIVGK
                     VVKRAEAKHLKLTELSLADWKEISSLFDNDLFRIFSLESAVNARAVWGGTASEQVLKQ
                     LAYAKSKMNTI"
BASE COUNT         2773 a         1961 c         2260 g         2414 t
ORIGIN      
        1 tttcctttct tctgacggag ttccattcct tttaggaatt gactgaaaaa ccaaatactc
       61 aggaaagaaa aaagcatcgg catagcttct aatctggctt gtgctccgaa ttcttccgcc
      121 agagaatgga tcataaagaa aaaagcggcg aaagggtgga tcatgacagc caatacttga
      181 actgtccaga gactgaaaac ggcactgact attcgccccg taaaaattgt gtaatcctct
      241 tttgccaaca tagcattgtt tcgggtattt aaaccgaatc gctgataata aggacgtccg
      301 tccagaataa cgggaatggc aaatgtcaat ttgaccaaag ccggatgttc atgattatag
      361 gtaattgagg ggatttcttt ccattgaaaa gagagagctt tgtcggcata gcgcatggcg
      421 gcccgagaat atgttccctc atcccatccc aacgggaaag tgtatacact aaccacacgt
      481 atcagtacag tagagagaga aatgatgata aacgcggttt taaacgcttt tgtacgtttc
      541 cggacaaatg cgtaaaccaa taccaaataa agcatcacca aagcgaatat gaaagagact
      601 acggaaagta aattgatcag aaaattgaat aattcaaacc ttccggcgga tggcccgtaa
      661 tcatgagaaa ttcggagaga atcttcttcg ttcatcgggc ttgacagatc ataggattcc
      721 gatgatccgt ctccccaatc gatccggatc tttccggacc ggggaccgac cataaaataa
      781 agatgaatgt ttctacccgc tttccctttg aattcaagtg cgtcgccgtt ttgaaaattc
      841 tcgagtacca actgatcatt ttcccttttc caatctccgt atgtcgaaaa gtccgataaa
      901 gaaattcccc ggtacccgtt atagagagag aggaattgaa ttttggtttt atccgccaaa
      961 gcctgatccg ggaagacatc gatatgaatt tttcgattcg gtaaaacaat ttctttttgc
     1021 ggaaaactca aatgaaaaag gaaagtaatc agaaccgata aaaatacgga agcaacaata
     1081 aaaataccga tcattttatg attaatcgat attttttttg caagaaatcg aatgaaaaag
     1141 aaaagaaaaa aagtaccggc taagaaaaaa ccgatcaaca acgccgtaag ttttgtattg
     1201 aaatgcatca acggcagcat ttgataaagt ccgagcgtag cggcggctga tatcaatccg
     1261 gaaggaatca agaatcgcaa cgaatgcttc aacgaatgct tcatagtgga gaattataac
     1321 cagttcattg atataattac attttgatcc ttatcgggaa tctgaatgaa aaacatattc
     1381 ttgatatgat ttataagaaa aaaccgagaa aaaattcaaa agatgccgaa tccgaatcaa
     1441 ccccaacccg atcccaaaca accgaatcat aatcaaggga aaccgaaccc gtcaggatat
     1501 ccatatcctc cagccggcaa tccgggaaat tggccatggc agcccaacga ccgggtttcg
     1561 ggatatccgc cgaatcggca atattcgaat ccgcaaaaac aaaattcgac gaacccttcg
     1621 ctcaataaca atcccaagaa aaaaccggga ccgaaaaagg atcgaatgga tgaattcggc
     1681 cggatattta ctatgattct ttccgctttt attttgattt atatgggttg gttatttgct
     1741 ccgcgaattc gaaacagtat gacattttgg ccgaccaaaa caccgactcc ggttacccct
     1801 tcgcctacgc tgatttccac accgacgatt actcctttag cttccaacac gccgactcta
     1861 atgccgaccg aaacgcccgg gcctctttcc gaatattgga ttgcggatgg agaatcgatc
     1921 gatccggctg ttcccgattc tcccgacggc gtcatcgttt tgcatgcgga taaaaacgcg
     1981 gaggtcaatc cgcctttgga tgatgtcact tggacgagtt ccgaaaagat cgcttcagat
     2041 ttgggaaaat ctacctatac cgaaaattgg tttgcgactt attccagcgg ttggatacgt
     2101 tggtttatgg atcagcctct tcgggaaggg ttgtatgaaa tatacacgat ggataccatg
     2161 tacagttcgg gtggttcgtt ggattttgcc gtgagtttgg gagatcagtt attgactcct
     2221 ttaacgggat cgcaacatgt tgattttatg acaagtcaat atgatccggt tcaatccgcc
     2281 gatacctggc gcagcatcgg tatttattat gtcggacctt cgacggaagc attgaatgtg
     2341 actacttctt gggaggatcg ggacgaatat acgattgttg cggttgatcg attactcatt
     2401 gttccgcgtc gtgtatcgga tctcggcatg ctaaacagcc tctctgtcag cggaacgaaa
     2461 tatgtctggg atgatctgga agccgagata accggtaaag actatattat ttcacaaaaa
     2521 gagcaactct cttgggatga ttcttaccag ctggttcttg atccgaaaac ggatgtcgaa
     2581 attacatttc ccaataggga accctatccg atcggaaact ataagttata tgcatggatg
     2641 ccggcaagta agggcgggat aaccgccact gtaaaatttt acctggataa taccgttttg
     2701 ccttccgaca ccggtgagga atcggtctca tatacggcac cgaaagcgga tgcaggctca
     2761 tggatcgaaa tcggttcttg gacgaccgat aagtattatg aacgaccgcg gaaaattaaa
     2821 ctgaccatcg taattccgga aggttctaaa ggggagtttc cggtagattg ttttgcactg
     2881 atccataacg gattttgatc gttccgttca agaaattgat gaaagagaga aaatcccatg
     2941 aattttgttg aaaatggaat ttatttgaat tttgatatag cagctgacgg caccttgaag
     3001 ctgtatcatt tcggttcccg gccattcgat ccgaacacga ttttggatga gagtcaaaaa
     3061 acaggattcc gggttgtcga agtgcaaatg tccggaatcg accgtcccga agaacgtcac
     3121 ggcactaaat ttattgtgac cgcaccgggg tatcgattga aatatcaacg tcattccgat
     3181 acgcgtaatt ctttcggcag aaagcttgaa ttcgttttaa tcgatcatga aaccggtact
     3241 gtggtcacct gtcattatca atttttcgac ggtatatccg ttgtacgttg ttggacggaa
     3301 gtggaaaacc ggggaactga aaatcaaacg cttgaatatg tgagctcttt cgcattgaca
     3361 gggatcggga aagaaggact aaaaagtcgt gatgaaaaac tgacgctctg gattccgcat
     3421 aattcttggc agagagaaat acagtggaca aagcacaaac tgcctgagtt agggctttgt
     3481 ttaaatcagc ccgaaaaaat cagccgctcc tcaaaaacaa tcggtgtgat gaacagcgga
     3541 aattggtcga ccaaagaata tatcccgatg ggatttttag aaaataccga gacgggtgac
     3601 agtctgattt ggcagatcga gcataacggt tcttggcgtt gggaaatcag cgaccaaaac
     3661 ggtcatttat atttacaact gagcggtccc aatgaaacct ggtctcattg gagtaaagtc
     3721 ttaaaaccgg gcgataaatt tacctctgtt ccggtagcgg tcggaaccgt taccggggat
     3781 tttgaaaagg caattgccga aatgacctca taccgtcggg taattcgtcg accgaatgag
     3841 gataacgaaa agcttgcaat catttttaac gattatatgc attgcttgtg gtccaacccg
     3901 acaaccgaaa aggaaattcc tttaatcgat acggcagcgg aaatggggtg cgaatattac
     3961 tgtatcgatg ccggttggta ttctccgggc ttttggtggg acagtgtcgg ggaatggcag
     4021 gtcagcctgg aacgatttcc gggaggactt actgaggtaa tggattatat ccgttccaaa
     4081 gggatgattc cgggaatatg gctggagatc gaagtaatgg ggattcgttg tcgtttggct
     4141 gaacaggtac cggatgattg gttctttgta cggcatggaa aacgggtata tgatcgaagc
     4201 cgattccaat tggatttccg taatccggag gtgcgtgctt ttgccgatga agtaatcgat
     4261 cgatttgtaa acgaatacca tgtgggttat atcaaaatgg attacaacat cgaaccgggc
     4321 atcggcacgg aagtcgatgc ggacagtttc ggggacggat tactgggaca tgagagagca
     4381 tacctgcagt ggattgacga tatttttgag cgctatcccg atttgattat cgagaactgc
     4441 tcaagcggcg gtttacggat ggattacgct atgttgagtc gtcacagtat ccaatccacc
     4501 agtgatatgg ataacgatcg aatggttgcg actatctctg ccaatgcgcc ttctgtatta
     4561 actccggaac aagccgctgt ttggtctttc ccgctggcgg gagatgatcg tgaggaagta
     4621 atctttaata tggtgaatgc aataatgttg cgcgttcatc aaagcggaca aattacgcaa
     4681 atttctcccg atcggaaagc gttggtgaaa gaagcgttgg atacttataa acggattcga
     4741 ccggatattc gccgggcatt tcctttttgg ccgctaggac tttcgaaata ttccgatcct
     4801 tgggtctgta tgggattgag aacagaccgt cgtgcgtata ttgctctttg gcgcaggaac
     4861 agcgagcagg aaacgatctc tttaccggtt tcatttttgg caggaaggtc ggtcaaagcg
     4921 aacattttat atccgtcttg cgatccatgt ccttattgct ggaatgatgg aacagccacg
     4981 ctcagcgtaa cattggaaaa gccttattcc gcgcggatat tctgtttgga attttgatct
     5041 acaaaaaatt cggattggag agaaggctta ttcagtgtat cccagatgaa taaaaaaaac
     5101 cttacgactt cctttcggat gatccaaaac caattgattt tccctgagaa ttcctccgat
     5161 ttggataaaa gttaacagat tacttatttt aaggtataat atcgaattga aaatggatat
     5221 taccgtgcaa tattcatggt acggcttatt tagggaatga aatcagccgg aaacggctga
     5281 tataaaaaca gaaagcaatg aatttaaagc ctgatcatca aagtgaaatt gatgggaatg
     5341 aaatgtaaag ggaaaaattc ataaaatttt ctacgtttta ccgatattcg gagaatttga
     5401 tcctatcggt tcagttaaaa aaataagccg tccgagagaa tattgtcgtg acggcttttt
     5461 tatatggacg gacgtgtgat tacgaagttg tcaattcaaa agagaaatcc cgggagagtc
     5521 aatgtctatc tggacgggaa gtttgcgttc ggaattccca aggatgctgc cgccggtttg
     5581 aaaacggggc aggaattgac agaaagtgaa attgaaaaac ttcaagaacg cgatcaggaa
     5641 gaccgggctt tatcaagcgc tttgagatca ctcaatcgcc gccttcaatc ggaaagtgaa
     5701 ctgcagaaaa aactgattca gaagggattc agcccggacc aaattgaatc ggttatggaa
     5761 agattgaaag agaatcacct gattgatgac aattcttttt ctgcgatgtg ggttgaaaat
     5821 cagactgcgt tccgtccgag aagcaagcgg atgcttactc tcgaattaaa aagaaaaggc
     5881 gtctccgaag aaaatatccg tgaatctttg aaggatctgg atgaggaaaa ggcagcgttg
     5941 gattgtggcc ttcgctacgt gaaacgatta caggggttag accgaccgaa tttccaaaaa
     6001 aagctgagtg catatttggc gagaagagga ttcggatatg aactaatcga tcagacggtg
     6061 catactcttt gggcagacat ccagaattcc gaaaaagatt ccatactttg aaataaagga
     6121 aaatgctatg tatggaaatt tttatccagg attaggagat aacggctttg gattcttatc
     6181 tgtatccgcg caaacccgac cgccggagac cggatccaac ctcctgatca taatcttagc
     6241 cgttgtaatc ggattgctgt tggtggtgac ggcaactttc tttatcaccc gatatttgaa
     6301 taccaaattt atgaagtctc agcagcttga tgcggagaat attatccttg tcagccgcca
     6361 aaaggcacag gaaattgaag aggaagctcg ggaaaatgcc aatcaaatcg ccaaagatgc
     6421 cgagacggaa ataaagcggc ggcgaggaga attaacacgc gaagaagacc ggcttcaaaa
     6481 acgccgggct gaaatcgatg cccggatcga aaaattggag cagcgggaag cgaacctgaa
     6541 taagcgccaa agcaaccttg ataagcgttc aaacgagatc gataaaatgg ctgcaaagca
     6601 gttggaagag cttcagaaaa tcgctcagat gacgacggaa gaggcgcggc aagagttatt
     6661 aaatatggtt gaaaaagatt cccgtaatga tatggctcga attattcgcc aaatcgaaca
     6721 ggaagcccgt atggacggtg aaaagaaagc ccgcgaaatc attgccaccg cgattcaaag
     6781 aatcgcttcc gatcaggtca gcgaagtgac gacctccatt gtcaatctcc cgggcgaaga
     6841 aatgaagggg cggattgtcg gcaggaatgg acgaaacatt cgtgctttcg aacaggctgc
     6901 cggagtggat gtcattgtgg atgacacacc ggaagcggtg atgatttcat gttttgatcc
     6961 aattcgcagg gaagtggcgc gccgagcgtt ggatcgattg attttggacg gaaggattca
     7021 tccggcaaac atcgaaaaaa cattgaatga agaagaaaag gaagttcaga agctgatcat
     7081 cgaagcgggt gagcaggccg cattcgaagc cggtgtggtc ggcttgcatc cggaaattat
     7141 taaaatgctg ggtcgattaa aatatcggac ttcgtacggg caaaatcagt tgtctcattc
     7201 ggttgaggtt gctaaacttt catcggtaat cgcatccgaa ttgggtgcca atgtggaatt
     7261 agccaggttg ggcgcattgt tacatgatct cggaaaagcg atggatcata ataccgaagg
     7321 gactcatgca aaaatcgggg cggaattcgc gaaacgatac ggtgtaaatc ccaaagcgat
     7381 caatattatc gaatctcatc accatgaggt tgatcaggaa agtgttgagg cagtaatagt
     7441 ggaagcggcg gatgcgattt cgggcgcgag accgggagcc agaagagaag atcttgagct
     7501 ttatatcaaa cggctgcgcg ctttggaaga aatcgcaaat tctcataaag gcgtacaaaa
     7561 ttcttatgca attcaagcgg gtcgcgaaat tcgcattatt gtgacaccgg aggaagtaga
     7621 cgatttggaa gccacttatt tggctcgcga tatcgcacat caagtcgaag aaaccatgca
     7681 atatccgggg cagataaagg tgacggtcat ccgtgaaacg cgggctgtgg actacgcaaa
     7741 gtagaaaact gaaatccgct gaccattaaa ataaaaagga tctttcgatg tagctaaccg
     7801 agagatcctt ttctttcata cgaactcgaa atcaaatcgt gttcatcttg cttttcgcat
     7861 aagccaactg cttcagcacc tgttcgctgg ctgttcctcc ccagacggca cgggcattga
     7921 cggctgattc caacgaaaaa atccggaaca aatcgttgtc gaataaggat gaaatttctt
     7981 tccaatcagc caaggagagt tcagtgagtt tcagatgctt cgcctcggca cgttttacca
     8041 ctttcccgac aatatgatga gcttcccgga aggggacgcc tttttttacc aggtaatccg
     8101 cgacatccgt tgcgaaagcc tgagtatcga cagcctgacg gcagcgatcg gcattgacat
     8161 gaagtgtatc gaccacgccg gttaatactt tcatcaacag caacgtgtta tcccaggatt
     8221 gaaagaacag gactttatct tcctgaagat ctttgtcata tgcggagggc agtcctttta
     8281 acgttgaaag catcgcttgc agtaagccga tctggctgcc gcattttccg cgaatgagtt
     8341 cgagtgaatc gggatttttc ttttgcggca tcagactgga tccggtcgaa tagcgatccg
     8401 ataacgtgat aaaaccgaat tccgttgtgg aatagagaat caacgcttcc gcacatcggc
     8461 tcatatgcac agcgatcatt gccatttcgt ataaaaaatc gcagacaaag tcccgatcgg
     8521 atacggcatc cagactgttg ggagcgggtt cggaaaaccc caagtccttt gccagtttga
     8581 atcgatcgat tggcagcgtg gatcctgcca aagccccaca tcccagcggt gagatatccg
     8641 cgtgactctg cgcttccgtt aatcgctgtt tatcgcgttc aagcgcccaa aaatgggata
     8701 accaccagtg gctcagtaag ataggttgcg cttgctgaaa atgggtataa ccgggcagga
     8761 tcactcccat gtcttgttcg gcacggtcga gaaacgcatt ttgtaaagtt tcgattcccg
     8821 acttcatttc ggaaatatga tttttgatcc ataaacggaa atcggtaacc acctgatcat
     8881 tgcgactgcg tccggtatgg atttttccgc cggcgggtcc gatgatttcg gtcagccttc
     8941 gttcgacagc cgtatggata tcctcgtcgg aagatttgaa ttgaaaagag tcgttcgaaa
     9001 attcttcgga aatctgtttc aacccggaaa tgattgaatc atattctgac cggctcagaa
     9061 cgccggcttc aaaaattgcg tttgcccaag ccagacttcc ctgaacatct tctttcgcca
     9121 ttcgttgatc aatccataaa gaattgttca gttgaatcat ctctcgatcc atttcactgt
     9181 ccaatcttcc gccggaccaa agtgtttgct ctttcatgag aattccttcc cgaaaaaatt
     9241 cggatcatta tttttccgaa caacctcaaa tgcgccggaa ttcggtttac ttgttttcaa
     9301 tctgttgttc catcattgcg cgaaccctgt tgggaagtgt aaagcaacga atgaatccgc
     9361 tggcatcttt atgatcgtaa agggatacat caccgaaaga agccattc
//
LOCUS       DAOMIV010000012         9279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_7505_length_9279_cov_5.063638, whole genome shotgun sequence.
ACCESSION   DAOMIV010000012 DAOMIV010000000
VERSION     DAOMIV010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7505_length_9279_cov_5.063638"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /gene="rpsC"
                     /locus_tag="PKV59_00610"
     CDS             <1..283
                     /gene="rpsC"
                     /locus_tag="PKV59_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="forms a complex with S10 and S14; binds the lower
                     part of the 30S subunit head and the mRNA in the complete
                     ribosome to position it for translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQP45279.1"
                     /translation="MKRAIQQGLRQGAEGVKIQVGGRLSGADMARTVTLREGRVPLQT
                     LRADIDFARAEANTTYGKIGIKVWIYRGIVLSMNEEAAAESTEGVYVSE"
     gene            351..785
                     /gene="rplP"
                     /locus_tag="PKV59_00615"
     CDS             351..785
                     /gene="rplP"
                     /locus_tag="PKV59_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQP45280.1"
                     /translation="MLMPKRVKYRKQQRGRMRGKALRGAEITFGEYGLMALEPGWVTA
                     RQIEAARRTLAREMKRHGKVWIRIFPDKPYTHRPPETRMGKGKGNVEYWVAVVKPGRI
                     MFEIGGLGEEAALEALHQAAYKFSIRTKVVSRHELGTGEEQL"
     gene            782..994
                     /gene="rpmC"
                     /locus_tag="PKV59_00620"
     CDS             782..994
                     /gene="rpmC"
                     /locus_tag="PKV59_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQP45281.1"
                     /translation="MKAAEIRLLTKDEITAKLLDLRQEKMNLRFQAVSGQLSDLSRVK
                     EVRRDIARMETILREMEMQKQQEGKA"
     gene            991..1305
                     /gene="rpsQ"
                     /locus_tag="PKV59_00625"
     CDS             991..1305
                     /gene="rpsQ"
                     /locus_tag="PKV59_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQP45282.1"
                     /translation="MNARRRIMGTVTSDKMDKTVVVEIRRSYRHPLVGKVVHDVKRVK
                     AHDENNECKVGDKVMIVESQPLSKEKRWVVQSVIRRDEKKEIIEEVSSELAEAMNEGA
                     KE"
     gene            1302..1673
                     /gene="rplN"
                     /locus_tag="PKV59_00630"
     CDS             1302..1673
                     /gene="rplN"
                     /locus_tag="PKV59_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQP45283.1"
                     /translation="MIQKESRLKVTDNTGAKEILVIHIMGGSVHHYGHVGDIVVGTVK
                     SASPHGSVKKSDIVKAVIVRCSKEWRREDGSTIRFDDNAAVILDVNGTDPKGTRIFGP
                     VARELREKGFTKIVSLAPETI"
     gene            1697..2035
                     /gene="rplX"
                     /locus_tag="PKV59_00635"
     CDS             1697..2035
                     /gene="rplX"
                     /locus_tag="PKV59_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQP45284.1"
                     /translation="MKLKIRKGDTVEIISGRSEDKKKRGEVIRVIPKESRIVVQGVNI
                     RSRHQGQTRAGRRQIDAGIVKFEAPIHISNAMLVCPNCNKPTRVGLSRGEDGKVTRIC
                     KKCNSEIDAK"
     gene            2052..2594
                     /gene="rplE"
                     /locus_tag="PKV59_00640"
     CDS             2052..2594
                     /gene="rplE"
                     /locus_tag="PKV59_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQP45285.1"
                     /translation="MNHMKEKYLQEVLPAMKSTLKLDNPMQYPKIEKVVVNIGLGEAL
                     DNPKALDAAVNDLAQITGQKPVVTKARVSIANFKLREGRQIGAKVTLRGEKMWGFLDR
                     LLNIVLPRVRDFRGVPTDAFDGRGNYTLGLKEQTLFPEIDYEKVDKVRGMEVTIVTTA
                     QNDDQARVMLDLLGMPFRKD"
     gene            2596..2778
                     /locus_tag="PKV59_00645"
     CDS             2596..2778
                     /locus_tag="PKV59_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HQP45286.1"
                     /translation="MAKKCMMYRELRREYPNQVRNRCKRCGRPRGYMRRFGLCRICFR
                     ELALNGQITGVTKSSW"
     gene            2801..3211
                     /gene="rpsH"
                     /locus_tag="PKV59_00650"
     CDS             2801..3211
                     /gene="rpsH"
                     /locus_tag="PKV59_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQP45287.1"
                     /translation="MVNDPIADMLTRIRNAMMAGHATVVMPCSNIKIEIARILKEEGF
                     LESYEVLEGDKPSQRALKLKIKYAGERRSRRPVMSGIVRVSRPGRRVYTGRNEIPWVL
                     SGLGVAIMSTPKGVMTGQRARQLGVGGEILCKVW"
     gene            3229..3777
                     /gene="rplF"
                     /locus_tag="PKV59_00655"
     CDS             3229..3777
                     /gene="rplF"
                     /locus_tag="PKV59_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQP45288.1"
                     /translation="MSRIGRLPITVPAGVKVDINGNEVTVKGPKGEMKRLFSPVVKID
                     QDNGTLTVTRSSDEAAIRALHGTSRAILNNMVVGVSQGFTVILEVDGVGYRAEMNGKN
                     LMLYVGYSAPKEVVPPEGISFDVDTKTRQIKVMGYDREQVGQVAAEIRFIRPPEPYKG
                     KGIHYLGEKIRRKAGKSGKAKK"
     gene            3790..4155
                     /gene="rplR"
                     /locus_tag="PKV59_00660"
     CDS             3790..4155
                     /gene="rplR"
                     /locus_tag="PKV59_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQP45289.1"
                     /translation="MAKFSRSEARIRRHARVRKDLAGSAQRPRLSVYRSLNGIYAQII
                     NDETGTTLVSASNIDRGLKADMDGKNKTEQAKLVGKAVAERAKEKGITTVVFDRGGFR
                     YMGRIKALADAAREAGLEF"
     gene            4179..4709
                     /gene="rpsE"
                     /locus_tag="PKV59_00665"
     CDS             4179..4709
                     /gene="rpsE"
                     /locus_tag="PKV59_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002063426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQP45290.1"
                     /translation="MENLDYQPLDEQLDERVIEIARVAKVVKGGRRFQFRVTVVVGDN
                     NGTIGLGIGKANAVPDAMRKASERARKNMRKINLYKTTIPHEVIGHVGGAQVLLRPAS
                     EGTGVISAGGVRAVLEVAGIHDILTKSLGSSNLLNVVKATFEALNELKNAEEEAILRG
                     KQPDELLPFWERRKNG"
     gene            4702..4908
                     /gene="rpmD"
                     /locus_tag="PKV59_00670"
     CDS             4702..4908
                     /gene="rpmD"
                     /locus_tag="PKV59_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQP45291.1"
                     /translation="MDKDTSSPKKLRITYVKSAIGYSWKHKDTIKALGFKHLNDSIVR
                     EDTPSLRGMLRKVNHLVKVEELAE"
     gene            4911..5363
                     /gene="rplO"
                     /locus_tag="PKV59_00675"
     CDS             4911..5363
                     /gene="rplO"
                     /locus_tag="PKV59_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQP45292.1"
                     /translation="MKLDELRPNEGAKHQKKRVGRGLAAGQGKTAGKGTKGQHVRQGY
                     GGHIYRQGGNLPFYRHLPFMRGEGFTPPNRVEFNEVNLEMLERFDGDIEITPEVLAEA
                     NLLSKKNNPVVILGNGEVSHAYKVKVHRISKGAREKIEAAGGSVEIIG"
     gene            5412..6749
                     /gene="secY"
                     /locus_tag="PKV59_00680"
     CDS             5412..6749
                     /gene="secY"
                     /locus_tag="PKV59_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQP45293.1"
                     /translation="MKRSPWRFLWKSTDIRKKLMLTLGILVLYRLIANIPVPGFNAEV
                     IKALKSNTTAAGDLISILNVLSGGTLSSFSIMAMGVYPYITASIILQLLGPIIPAIER
                     KMKDDPREGQKWMEKWTMILTIPMAILSAIGQISLFNQILPGTTVLRDYGWSGSNFLP
                     TLANLSTMVAGTMVAIWLGQIISEYGIQQQGLSLIIFSGIVSQIPNNLVRILADKTTG
                     WIVLVISLVVLLISVFAIVYIQQGTRYVKLMIPGQRIGYRGIKSGGSTTLPLRVNSVG
                     MIPLIFAQSIITFPALIASFFVNSGTAWVKNSSVWIQSHLSGSSSLYVVLYFVLVVAF
                     TYFYTSVTFMNNDYGSSLKKQGAVVPGRAAGTETQKYLAKIQNRITLPGAVMLGVIAV
                     LPNILAVILHTSAQATMLMSSSGMLIVVGVVRDIIDYLQSELSQHGYEEKLIR"
     gene            6773..7435
                     /locus_tag="PKV59_00685"
     CDS             6773..7435
                     /locus_tag="PKV59_00685"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQP45294.1"
                     /translation="MATYIVFLGAPGVGKGTQAERLAEELKIPHISTGDLFRENLKNQ
                     TELGKKAQYYMDAGQLVPDDVTIEMVKSRISRPDCVNGAILDGFPRTSAQAEAFDQML
                     KEAYQTEVDFVPCIQVDRELLIDRLSGRRMCPNGHVFHMVYHPPKEDDICDICGQKLY
                     QREDDKAETIEKRLEVYENQTAPLIDYYQKRNVLRFIDGAKDIDAVTMDILAAFKDVT
                     KK"
     gene            7452..8270
                     /gene="map"
                     /locus_tag="PKV59_00690"
     CDS             7452..8270
                     /gene="map"
                     /locus_tag="PKV59_00690"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQP45295.1"
                     /translation="MSWERQVSIKSKDEIAIMREAGIINAEALKAASEVCVPGATTWD
                     VNAAADRVLDHYKVTSPFKGVPGPIPFPASTCVSVNDVLVHGIPSKKIVLHEGDIVSV
                     DCGTTYKGFVADSAFTIGVGKVSEEAQKLMEVTENSLYIGIEKMIPGNYTGDVSAAIQ
                     EYVESHGFYVTKQYTGHGVGRRMWEAPQVPNYGTKGQGIRLRPGITIAIEPMVLVGTD
                     ATQVHQDGWAVSSADLSLTAHFEHSIAVTEEGPMILTLLENGEPPVSVTGRKKL"
     gene            8370..8483
                     /gene="rpmJ"
                     /locus_tag="PKV59_00695"
     CDS             8370..8483
                     /gene="rpmJ"
                     /locus_tag="PKV59_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQP45296.1"
                     /translation="MKVTSSIRKRCAKCKIVKRSGRLYVICENPKHKQRQG"
     gene            8490..8879
                     /gene="rpsM"
                     /locus_tag="PKV59_00700"
     CDS             8490..8879
                     /gene="rpsM"
                     /locus_tag="PKV59_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQP45297.1"
                     /translation="MARIEGVDLPRNKRIEIGLTYIYGIGLTRAQQALRDTGVNPDIR
                     VKDLSENDENLLREYIAKNYVVEGDLRREVQMNIKRLVEIGCYRGIRHRRNLPCRGQR
                     TRTNARTAKGPKKTVAGRGRRRGATKK"
     gene            9001..>9279
                     /gene="rpsK"
                     /locus_tag="PKV59_00705"
     CDS             9001..>9279
                     /gene="rpsK"
                     /locus_tag="PKV59_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQP45298.1"
                     /translation="MAQNVRSSRRASTARKVKRTLGSAQVHIFASFNNTIVTVTDAMG
                     NPVCWSSSGASGYKGSRKSTPFAARLAAEQAIKTAQTMGVQEVDLIIKG"
BASE COUNT         2651 a         1872 c         2512 g         2244 t
ORIGIN      
        1 gatgaagcgc gccattcagc aaggcttacg ccaaggagct gaaggtgtta agattcaggt
       61 cggcggtcgt ctgagcggtg cggatatggc tcggacagtg accttacggg aaggccgtgt
      121 gcctttgcag actttgcggg cggacatcga ttttgcccga gctgaagcaa acaccaccta
      181 tggaaaaatc ggcatcaaag tttggattta tcgcggtatc gtgctttcca tgaacgaaga
      241 agctgctgct gaatccactg aaggtgttta cgtcagtgaa taagacgaat actgatcacg
      301 tttacgggat caccgtgaac gttggggtta attgaaagag gtattgaaat atgttaatgc
      361 caaaacgtgt gaagtaccgc aagcagcagc gtggtcgaat gcgcggaaaa gctctgcgcg
      421 gagcggaaat taccttcggg gaatatgggc tgatggctct ggaacccggc tgggtaaccg
      481 ccaggcagat cgaagctgca cgccgtacat tggcgcggga aatgaagcgc cacggaaagg
      541 tttggattcg aatctttccg gataaaccgt atacacatcg acctcccgaa acccgtatgg
      601 ggaaaggaaa agggaatgtc gaatactggg ttgcagtcgt taaaccggga cgaattatgt
      661 tcgaaatcgg tggtttggga gaagaagcgg cattggaagc attgcaccag gctgcctata
      721 aattttccat tcgcaccaaa gtagtttcac gacacgaact gggcacagga gaagaacaat
      781 tatgaaagca gctgaaattc ggttattaac gaaagatgaa attacagcca agctattgga
      841 tttgaggcaa gaaaagatga atcttcgttt tcaggctgtt tccggccaat taagtgatct
      901 tagtcgtgta aaagaagttc gccgtgatat tgcccggatg gaaaccattc tgcgagaaat
      961 ggaaatgcag aagcaacagg aaggtaaagc atgaatgcac gccgtcgaat aatgggtact
     1021 gttacaagtg acaagatgga taaaaccgtt gtcgttgaaa ttcggcgatc ttaccgtcat
     1081 ccgctggttg gcaaagttgt ccatgatgtg aaacgtgtca aagcacatga tgagaacaat
     1141 gaatgtaaag tcggagataa agtgatgatt gttgaaagcc aaccgctttc gaaagaaaag
     1201 cgatgggtgg ttcaatcggt cataagacgg gatgaaaaga aagaaatcat cgaggaagtc
     1261 tcgtccgaat tggcggaagc gatgaacgaa ggagccaaag aatgattcaa aaggaaagca
     1321 gattaaaagt gactgataat acaggcgcca aagaaatcct tgtgattcat attatgggtg
     1381 gttccgtcca tcattatggt catgtgggcg atattgtcgt cggtacggta aagtccgcat
     1441 ccccgcacgg atctgtcaaa aagagtgata ttgtgaaagc cgtaatcgtt cgctgctcca
     1501 aggaatggcg acgggaagat ggctcaacga tccggtttga tgataatgca gcagtgattc
     1561 tggatgtcaa tgggacggat ccgaaaggga cacgtatttt tgggccggta gcgcgtgaat
     1621 tgcgtgaaaa gggctttaca aagatcgttt ccctggcacc ggaaactatc tgagcattgg
     1681 aaagatggag cgaagaatga aactaaaaat tcgcaagggc gatacggttg aaattatcag
     1741 cggccgttcg gaagacaaaa agaaacgtgg cgaagtaatt cgcgtcattc caaaggaatc
     1801 gcgaatcgtt gttcagggag tgaatatccg ttcacgtcat cagggacaga cacgtgccgg
     1861 acgtcgtcaa attgacgcgg gaattgtcaa atttgaggca ccgatccaca tttcaaatgc
     1921 catgttggtg tgtccgaact gcaataaacc gacccgagtt gggttaagcc gtggtgaaga
     1981 cggtaaagtt accagaattt gcaaaaaatg caattctgaa attgatgcga aatagcggga
     2041 gcaaacagat catgaatcac atgaaagaaa aatatttgca agaagtactt cctgctatga
     2101 aatctacatt aaagttggat aatccgatgc agtatcccaa aatcgaaaaa gtagtcgtaa
     2161 atatcggttt gggtgaagct ttggataatc ccaaggcatt ggatgctgcg gtcaacgatc
     2221 tggcgcagat tacgggtcag aagccggttg tcaccaaagc ccgagtcagc atcgcaaatt
     2281 ttaagctgcg tgaaggtcgt caaatcggcg cgaaagttac tttacgcggt gaaaaaatgt
     2341 ggggcttttt agaccggctg ctcaatattg ttttgcctcg tgtgcgcgat tttcgcggtg
     2401 ttcctacgga tgcttttgac ggtcgcggga actacacatt ggggctgaaa gaacagactc
     2461 tgttccccga aatcgactat gaaaaagtcg ataaagtccg cgggatggag gtcacaatcg
     2521 ttacaacagc tcaaaatgac gatcaggcgc gcgtaatgct tgacttgctc ggcatgcctt
     2581 tcaggaagga ttaatatggc taagaaatgc atgatgtatc gtgaactcag gcgtgaatac
     2641 cccaatcagg ttcgcaatcg ctgtaagaga tgcggcagac cgcggggtta tatgcgccga
     2701 tttggattgt gcaggatttg cttccgcgaa ttagcactga acggccaaat caccggcgtt
     2761 acgaaatcat cctggtaatc cataacggag gaatagaaag atggttaatg atcctattgc
     2821 cgatatgttg acacggattc gcaacgcaat gatggctggt catgctacgg ttgtaatgcc
     2881 ctgttcaaat atcaaaatcg aaatcgcacg gattctgaaa gaagaagggt ttttggaatc
     2941 ctatgaagtg ctcgaaggcg acaaaccttc tcaacgagcg ctgaaattga aaatcaaata
     3001 tgcgggtgaa cgacgctcac gccggccggt tatgtccggt atcgtccgtg tcagtcgccc
     3061 cggaagaaga gtctacaccg gccgaaacga gatcccctgg gttttgtcgg gcttgggtgt
     3121 agcgattatg tccactccga agggagtgat gaccgggcag cgcgcccgcc agttgggtgt
     3181 gggcggcgaa atcctctgca aggtctggta ggaaaggaat taaaggaaat gtctcgaatt
     3241 ggacgtttac ccattaccgt tccggcaggc gttaaggtgg acatcaacgg gaatgaagtg
     3301 acagtaaaag ggccgaaagg ggagatgaaa cgcctctttt ctccagttgt caaaattgat
     3361 caggataacg gaactcttac tgtgacacga tcctcggatg aagctgctat tcgcgctctt
     3421 cacggaacga gtcgggcaat tttaaataat atggtcgtag gtgtttctca gggatttacg
     3481 gtcatcctcg aagtggatgg tgtcggatat cgagccgaaa tgaacgggaa aaacctgatg
     3541 ctttatgtcg gttattcagc cccgaaagag gtcgtgccac ctgaaggaat atcttttgac
     3601 gtcgatacaa agacgcgtca gataaaagtc atgggctatg atcgtgagca ggttgggcaa
     3661 gttgctgcgg aaatccggtt tatcagaccg ccggaacctt ataaaggtaa aggaattcat
     3721 tacctgggtg aaaaaatccg ccgtaaggcc ggtaaatccg ggaaagctaa gaaatagagg
     3781 taataaaata tggcgaaatt ttctcgaagt gaggcaagaa ttcggcgcca tgccagagtc
     3841 cgtaaggatt tggccggaag cgcgcaacgc cctcgtttaa gtgtttaccg aagcctaaac
     3901 ggtatctatg cccaaattat taatgatgag accggcacaa cgctggtatc cgcatccaat
     3961 atcgatcgcg gactgaaagc cgatatggat gggaagaata aaaccgaaca ggctaagctg
     4021 gttggtaaag ccgttgcgga acgcgcaaag gaaaaaggaa ttaccacagt cgtctttgat
     4081 cgcggcggat ttcgctacat gggtcgtatt aaagccttgg cagatgctgc gcgtgaagca
     4141 ggccttgagt tttaggcaaa aggaaaggta ctaaaaccat ggagaatttg gattatcaac
     4201 cgttagatga acaattagat gagcgtgtga tcgaaatcgc gcgtgtcgcc aaagtagtaa
     4261 agggcggacg ccgctttcaa tttcgtgtaa ccgtagtcgt cggcgataac aacgggacga
     4321 tcggtctcgg aatcggaaaa gccaatgcgg ttccggatgc gatgcggaag gcttccgaac
     4381 gtgcccgaaa gaatatgcgg aaaataaatt tatataaaac aaccattccg catgaggtta
     4441 tcggtcacgt aggcggtgcg caggtattat tacgccccgc atccgaagga accggcgtta
     4501 tttccgcagg cggcgttcgc gctgttttgg aagttgccgg gatacatgat attttgacga
     4561 aatcattggg cagttccaat ctgctcaatg ttgtcaaagc aacttttgaa gcgctcaatg
     4621 aattaaagaa tgccgaggaa gaagcgattt tgagggggaa acaaccggat gaattgctgc
     4681 ctttctggga acggaggaag aatggataaa gatacttcga gtcccaaaaa attgcgcatt
     4741 acctatgtaa aaagtgcaat cggctattca tggaaacata aggatacaat caaggcactg
     4801 gggtttaaac acttgaatga ttcgatcgta agagaggata ctccttctct tcgcgggatg
     4861 cttcgaaagg ttaatcacct ggtcaaggtt gaggaattgg cagagtaatt atgaaactgg
     4921 atgaattaag accgaatgaa ggcgcaaaac atcaaaagaa aagggtcgga cgcggtttag
     4981 cggccggaca gggtaaaaca gccggcaaag gtacaaaagg acagcacgtc cgtcaagggt
     5041 acggcggtca tatttaccgc caaggcggaa atttaccttt ttatcgtcat ttgcctttta
     5101 tgcgtgggga aggttttacg ccgcccaatc gggttgaatt taatgaagtg aatctcgaaa
     5161 tgctcgagcg atttgacggc gatatcgaga ttactcccga agtattggct gaagcgaatc
     5221 ttctcagtaa gaagaataac ccggttgtca ttttaggcaa cggagaagtc tcgcatgcat
     5281 ataaagtcaa ggtccaccgg atatcaaaag gtgcccgaga aaaaattgaa gctgccggcg
     5341 gcagcgtcga aattatcggc tgaagttttt ggctgaagcc gttcgctgga gttattaagg
     5401 agaactaaag aatgaagcga tccccatggc gttttctgtg gaagtcaaca gatattcgca
     5461 aaaaactgat gttgacgttg ggaatactcg tactctatcg tttgatagcg aatattccgg
     5521 tgccagggtt caatgccgaa gttattaaag cattgaagtc caacacaact gccgcaggtg
     5581 atttgatcag tatcctgaat gttttatccg gcggtacctt atcgtctttc tccattatgg
     5641 caatgggcgt ttacccgtac attaccgcgt cgattatcct tcaattgctc ggcccgatta
     5701 ttccggcaat cgagcgaaaa atgaaggatg atccgcgcga aggccaaaaa tggatggaaa
     5761 aatggacgat gattctgacc atcccgatgg cgattttatc ggctatcggc caaatcagcc
     5821 tctttaacca aatccttccc ggcacgacgg tgcttcggga ttacggctgg agcggaagca
     5881 actttctgcc gactcttgcc aacctcagca ctatggttgc gggaacgatg gttgcgattt
     5941 ggttgggtca gattatttcg gaatatggca tccaacagca aggtctgtcc ttaatcatct
     6001 tctccggtat cgtttcacag atacccaata acctggttag aattttggcg gataaaacga
     6061 cgggctggat cgttttggtt atctctctgg tagttttgct gatctcggta tttgcgatcg
     6121 tttatatcca gcaggggaca agatatgtga aactgatgat ccccggtcaa cggatcggct
     6181 atcgcggtat taaatccggc gggtcgacaa cgttaccgtt acgggtcaat agtgtgggga
     6241 tgattccgct catttttgct caatcgatta ttactttccc ggcattgatt gccagtttct
     6301 ttgtgaattc cggtacggca tgggtgaaaa attcttccgt atggattcag agtcacctca
     6361 gcggttcaag cagcctctat gttgttttat attttgtgct ggttgtcgcg ttcacttatt
     6421 tttatacaag cgtgactttc atgaacaatg attacggctc cagtctgaag aaacaagggg
     6481 cggtagtccc cggcagagca gccggaacgg aaactcaaaa atatctggcg aagatccaaa
     6541 accgaatcac tttgcccggt gccgtgatgc tgggtgttat cgcagttttg ccgaatattt
     6601 tggctgtcat actgcatacc agtgcacaag cgacgatgtt gatgtcctct tccggtatgc
     6661 tgatcgtggt cggtgtcgta agagatatta tcgattacct gcaaagtgaa ctgagccaac
     6721 atggctacga agaaaaattg atccgctgaa acagcgagat aagagaaaaa gaatggcgac
     6781 atatattgtc tttttgggag ctccgggagt gggaaaagga actcaggctg aaaggcttgc
     6841 tgaagagttg aagatccctc atatcagtac cggtgatctt ttcagagaga acctgaagaa
     6901 tcaaacggag ctcgggaaaa aagcacagta ctacatggat gcgggacaac ttgttccgga
     6961 tgatgtgacg attgagatgg tcaaatcgcg aatatcccga ccggattgtg ttaatggcgc
     7021 aatattagac ggatttccgc gaacttccgc acaagccgaa gcttttgatc aaatgctcaa
     7081 agaagcttat cagaccgaag tcgatttcgt cccatgtatt caagtcgatc gggaactgct
     7141 gatcgacagg ttgagcggtc gccgaatgtg tccgaatggc cacgtctttc atatggtata
     7201 tcatcctccc aaagaggatg atatctgcga tatttgcggt caaaaacttt accagcgtga
     7261 ggatgataaa gcggaaacca tcgaaaaaag acttgaggtt tatgaaaatc agacggcacc
     7321 gttgattgat tattatcaaa agcggaatgt attgcggttt atagacgggg caaaagatat
     7381 tgatgccgtt actatggata ttcttgcggc attcaaagac gtaaccaaga aatgaccggg
     7441 aagctgatag aatgagctgg gagaggcaag tcagtatcaa atcaaaagac gaaatcgcga
     7501 ttatgcgaga agcgggcatt attaatgccg aagctttaaa agccgcgagc gaggtttgtg
     7561 ttccgggtgc gacaacctgg gatgtgaatg ccgcagccga cagagttctg gatcattaca
     7621 aagtaacttc tccttttaaa ggagttcccg gtccgattcc gtttccggca agcacttgtg
     7681 tcagtgtgaa cgatgttctg gtccacggaa tcccgagcaa gaaaattgtt ttgcatgagg
     7741 gagatattgt ttccgtggac tgcgggacaa cctacaaagg ttttgtggct gattcggctt
     7801 ttacgatcgg tgttggaaaa gtctcggaag aagcgcagaa gctgatggag gtgacggaaa
     7861 attccttata tatcgggatc gagaaaatga tacccggcaa ttacacggga gatgtttccg
     7921 ccgcgattca ggaatatgtt gaaagccacg gattttatgt gaccaaacag tacaccggac
     7981 acggcgtcgg gcgacgaatg tgggaggctc cgcaagttcc gaattacggg accaaaggtc
     8041 aaggaattcg attgcgaccg ggaataacca tcgcaattga gccgatggtt ttggtcggta
     8101 ccgatgcgac acaagttcat caggacggat gggctgtttc ttcggcggac ttgagcctga
     8161 ccgctcattt tgagcatagc atagcggtca cagaagaagg tccgatgatt ttgacgctgt
     8221 tggaaaacgg tgaaccgccg gtgtcggtaa cgggacgaaa aaaactgtaa tgagtatatg
     8281 attatctttg acagacaaag tcatgacgag tataattgtc tggctattaa tgttaagtcc
     8341 gggtggaaaa acatccgagg gagtttaaaa tgaaggttac ttcatcgatc agaaaaagat
     8401 gtgcaaagtg taaaatcgtc aaacgatcag gtcgtttgta cgttatttgc gaaaacccaa
     8461 agcacaaaca gcgacaggga taagaggata tggcgagaat tgaaggtgtc gatcttccgc
     8521 gtaataaacg gatagaaata ggtttgactt atatttatgg gatcggactt acccgagccc
     8581 aacaagcctt gcgggatacc ggtgtcaatc cggacattcg ggtaaaggac ttgtcggaaa
     8641 atgatgagaa tttgcttcgt gagtatattg ccaaaaacta tgttgtcgaa ggtgatttgc
     8701 gccgcgaagt tcagatgaat attaaacgcc tggtggaaat tggttgttac cggggaattc
     8761 ggcatcgtcg aaatttaccg tgccgaggac aaagaacgcg gacaaatgcc cgaaccgcaa
     8821 aaggtcccaa gaagaccgtt gccggacgtg gccgccgccg tggagcaacc aagaaataag
     8881 tcgaatttcg ctttggcaaa taattacagg ttgggatcct ttctccctgc ggctgcctgt
     8941 aattgtttgc tgtcgataat ttgattcttt tcagacataa ggttaaggta gaggaaattt
     9001 atggctcaaa atgtacgctc atcgcgccgt gcgtctaccg cacgaaaagt aaagcgcacg
     9061 ctgggatcag ctcaggtgca cattttcgct tcttttaaca acaccatcgt caccgtgacg
     9121 gatgccatgg ggaatccggt atgctggagc agttcgggtg cttccggtta taaggggtct
     9181 cgaaagagta caccttttgc tgctcgatta gctgccgaac aagcgattaa gaccgctcag
     9241 accatgggtg ttcaggaagt ggacttgata attaaaggc
//
LOCUS       DAOMIV010000013         9109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_7682_length_9109_cov_3.722774, whole genome shotgun sequence.
ACCESSION   DAOMIV010000013 DAOMIV010000000
VERSION     DAOMIV010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 9109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9109
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7682_length_9109_cov_3.722774"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKV59_00710"
     CDS             complement(<1..295)
                     /locus_tag="PKV59_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45299.1"
                     /translation="MFWLAVWQALSMIPGWNIFLASPIVVFKTAIQLVREPVFWTTVL
                     FSFTRIVAGFIFGLCAGIVFAILSVNFQIIRLLLEPIVLLIKSTPVASFIILVL"
     gene            complement(327..1313)
                     /locus_tag="PKV59_00715"
     CDS             complement(327..1313)
                     /locus_tag="PKV59_00715"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45300.1"
                     /translation="MKSNKLLIIIVSFLFAFFITVNTINFHVQSQDNVSQTIRIGALK
                     GPTGMGLAKLMEDSQAGDEKQFTLTGTADEIVTGLVKGELDFGAVPCNLASVLYHKTN
                     GGIKVAAINALGVLYVLENGNTIHTVQDLKGKTIYTTGKGTTPEYVFRYILTKNSIDP
                     DKDVEIIFKSESTEIASMFASEKNLIALLPQPYVTTVQMQQPDVRIALDLTQEWEKVS
                     PDHPLATGCVVVRTELTENDPETVKSVLSEMADSVSYVNQNVDDAATLIAKFGIIPNA
                     EIAKKAIPYCNITFIHGEEMQKALSEYLTILFEANPVSVGEKLPDEAFYFKY"
     gene            1556..1990
                     /locus_tag="PKV59_00720"
     CDS             1556..1990
                     /locus_tag="PKV59_00720"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQP45301.1"
                     /translation="MKVEIKILEDIIEPYAIIYTSALTDEIQKAATLISIQENAVPVM
                     LEEKIFMLKPDEIFMARVENNSLAIYSESRRYLCKKRLYEISEILGAGFMQISKSTVV
                     NLSEVDYIEPFFNGMVNLKLKNGLSDYISRKYMKDFKRYFGL"
     gene            2003..2440
                     /locus_tag="PKV59_00725"
     CDS             2003..2440
                     /locus_tag="PKV59_00725"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HQP45302.1"
                     /translation="MIEIIKRIIEGIVIGCTIFTIIGIIFCLVNGTDTAFGDPDYGFV
                     KMAICSMVVGLGFSLPTFIYDDQKITKPLQTLIHLSIGFIIFGLVAFYAKWVPVESGI
                     KEIVVFIILSILSTLAIWYCFYLYYKNEARKINDDIRKKQNKN"
     gene            2632..2928
                     /gene="rplI"
                     /locus_tag="PKV59_00730"
     CDS             2632..2928
                     /gene="rplI"
                     /locus_tag="PKV59_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQP45303.1"
                     /translation="MKVLLLKDVYKLGHAGDVKKVADGYARNYLIPQGLVTMATPGAI
                     KQADQIKQKADAIRSQLNTEMSSLFEKIWDGYNFCQQGRRNRQIIRINHSANDR"
     gene            2852..3127
                     /locus_tag="PKV59_00735"
     CDS             2852..3127
                     /locus_tag="PKV59_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal L9 C-terminal domain-containing
                     protein"
                     /protein_id="HQP45304.1"
                     /translation="MVITFASKAGETGKLYGSITPQMIVDAINEKSGASIEKRQLEVE
                     PIRTLGEHHVTVRLTVDIVPTVKVIVHREGEKVNLESLVTEEPEKAE"
     gene            3198..4538
                     /locus_tag="PKV59_00740"
     CDS             3198..4538
                     /locus_tag="PKV59_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQP45305.1"
                     /translation="MSVSVGKALRSARESKNIPLSKVSDDLYIRITYLQAIENGRPEI
                     LPSPVQGRGFVRMYSDYLKLDTEKILYAWDHPDIPFSLDSEMSNQTESPAPQEPIIEK
                     TESSTQPSKSDTANSQNADPVSAIYKQIGSELRQRREILGLSYEDAEKHTLVRSIYLK
                     LMEDGNFDSLPSSVQARGMLNNYAAFLNLDVENIMLQYANALQLKSASRIAETAKEKK
                     NKKEPKPTKKAGKFRQFMTPDLFVGVSVLIAMFAIIIYSAVTISEYKAKAVQKTVDLN
                     LNFINQLATHDITVTATSDFNETPTVPISEMNPSEPTQMNTQSENLTVEETNRPIQVF
                     VEANQRAFMKVVSDGDVVFTGRTLPGNTYPFDASQMIEVTTGNAAALKITYNQQSLGL
                     MGKLGEVITLQFTNNAIMTPTPAITMTPTLTLEPTYTPQVFEATPTFTITPYIP"
     gene            4552..5856
                     /gene="rimO"
                     /locus_tag="PKV59_00745"
     CDS             4552..5856
                     /gene="rimO"
                     /locus_tag="PKV59_00745"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQP45306.1"
                     /translation="MQKNTFHLISLGCAKNTVDSNTVANILQNAGYSFNIDPKQSEYL
                     IVNTCGFIQSARDEAVETLQGLAADKNPGQFLLAIGCMAELYSRQILEACPEVDGVLG
                     TRKITDILQLITGVKETDIQLNPVPEEILTSQFTIQGPAAYLKIADGCGRYCSFCSIP
                     LIKGSWISRPQADILADAVRLQEMGIKELILIAQDTTAYGIDRGEKDGLPDLIEKILS
                     AAPDIPRIRVLYAFPGFVSDRLIDLLSNNERVLPYLDIPLQHAHPEVLKRMHRPADMD
                     WVRKTIAKMRSANPDFAIRSTFIVGFPNETEEEFETLYDFLNEMKFDRVGVFSYSFEE
                     GTASAVYGDTVPEEVKRERLDRLMKLQQGISREINRKFIGRKMDVLVERQQDGLLIGR
                     SYRDAPDIDGLVFVEGKAKIGDIIKVRIHTALEYDLIGKRIG"
     gene            complement(5990..7312)
                     /locus_tag="PKV59_00750"
     CDS             complement(5990..7312)
                     /locus_tag="PKV59_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45307.1"
                     /translation="MNQNKSNSDKNPKTPKNSENTPNPSSQNYSGSYGSHIPQGEPTP
                     YDPSRSYYPQQQGYYPPPQYPQGQPSMPGNKNPQLQPKKNQKKAAKPKKQGNGKWVWL
                     GILIMLVLIAAGGVIGYQTGIQARQNSYKEQSIKAAAQQYNLAIADIQNGKYENAKTR
                     LEYVLSVDPNYPGATEKYTEVVVALYPKETPTPFYTPTPAPTSTPDTRGEEELLNTIR
                     ANMAAQQWETAIANIEALRNKNLSYKSIEVDGMYYIALRNYGIQLINNGYLESGIYKI
                     TLAEAFGPIDALASNQRDAARSYLAGAGFWEIDWAKALEYYSNAYLTAPGMYDRASGY
                     TAQERYIQASFEYANRLAQTGDYCASIEYYNQAFSMSANEQYAPTATAAYLECYPPTA
                     TPEVQIQQPTEMVFTPTVPGMSGGESGQVEIPPVDNSTEPIPEPETVP"
     gene            complement(7377..8981)
                     /gene="murJ"
                     /locus_tag="PKV59_00755"
     CDS             complement(7377..8981)
                     /gene="murJ"
                     /locus_tag="PKV59_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQP45308.1"
                     /translation="MKLSSLSRTSLMIVIFFGIDKVIAIIRQLIIARQFGLSAELDVF
                     NIANNLPDMLFILISGGAMAMVIIPIMTEIIQKESREASWRVFSSIANLAFVVSLILA
                     IFIAIFARPIVTSEIGIAPGFTTEQQEHVVNMMRLNLIATLIFSISGLVMGGLQANRK
                     FFLSALAPILYNIGQIFGAVVLSPQEPYRIGQIELPAFGMGIYGLAGGVILGAILHLL
                     IQIPGLIRLKFKWYPMIQWKDPYVQKTIRIMIPRILTVFFVQLIFIIRDNLASRLETG
                     SVTALSYGYMFQQLPETLIGTALGTAILPSLSLFISENKKAEFQKTFEIACRLSVALT
                     IGIAVIMGVGLGPIVQRVLGFNVAENALLMWTLRGFLVGLTGHCLLEVVNRAFYAQQN
                     ARIPLIGTILNLVIYIILGRILYRPLNAPGISLTDSVSFSLQALFLLLLLRMEPAERN
                     NRFYGKKGKQSAPSVPQMNAAPQLWNPIIRSAIGAGIAGIITYEAMHYNPVFSDNLVK
                     SIIGMGAAFLVYLIFILPEIKALRHF"
BASE COUNT         2742 a         1940 c         1890 g         2537 t
ORIGIN      
        1 ataaaaccaa aataatgaag ctggcaaccg gtgtcgattt aataagcaat acaatcggtt
       61 ccaataacaa tcggattatt tgaaaattta cagaaagaat cgcgaataca attcccgcgc
      121 ataagccgaa aatgaatccc gcaacaatcc gtgtaaagct gaataaaaca gtggtccaga
      181 aaacaggttc ccggaccaac tgaatagctg ttttaaacac gacaatcgga gaagccagaa
      241 aaatattcca acccggaatc atactaagtg cctgccaaac ggcaagccaa aacaatataa
      301 tccaaaatcg ccggaatgtt tttctctcag tatttgaaat agaatgcttc atcgggtagt
      361 ttctcgccca ctgaaaccgg atttgcctca aataagattg ttaaatattc actcaaagct
      421 ttctgcatct cctcgccatg aatgaaagta atattgcagt aaggaatagc ctttttggca
      481 atttcggcat tgggaatgat accgaatttt gcaatcaatg tagccgcatc atccacgttt
      541 tgattgacat aggacacgga atcagccatt tcagataata cactcttcac cgtttcggga
      601 tcattttccg tcaattcggt tcggactacc acgcatcccg tagccagcgg atgatcggga
      661 cttactttct cccattcttg ggtcagatca agagcaattc ttacatcggg ttgttgcatc
      721 tgaacagttg tgacataggg ttggggtaat aatgcgatca aatttttttc agatgcaaac
      781 atagaagcga tttcggtcga ttcactttta aaaatgatct ccacatcctt atcaggatcg
      841 atgctgtttt tcgtcaaaat atatcgaaac acgtattcgg gtgtcgtccc ttttccggtg
      901 gtataaatcg tttttccttt taaatcctga acggtatgaa tggtattccc attttctaat
      961 acatataaaa cgcctaaagc attgattgcg gcaactttaa tcccgccgtt cgttttatga
     1021 taaagaacgc ttgccaggtt acagggaaca gctccaaagt ccagctcccc cttaaccaaa
     1081 ccggtaacaa tttcatccgc tgttccggtc aaggtaaatt gtttctcgtc ccctgcctgt
     1141 gaatcttcca ttaactttgc cagacccatt cccgtcggtc cctttaaagc accgatccgg
     1201 atcgtttgag aaacattatc ttgggattga acatgaaaat taattgtatt aacggttata
     1261 aaaaaagcaa aaagaaaact aacaataatt atcaataatt tatttgactt catatcaact
     1321 cctgaaaata ttgcattttg cttattttac ctcattcgtg aaaatattat catttactat
     1381 gatttaaaaa aaggagaaca aatattatca aaatcaaatt ttgataatat tcatttataa
     1441 aattgctttc tcagatgatt tcttgcattt caacgcatat tttctgccac ttaccgattt
     1501 tttgctgacg aatgaaacgc tatatgttaa tatttgctca aaaggagaaa attcattgaa
     1561 agtcgaaatc aagatattag aagatattat cgagccatat gcaattatat atacaagtgc
     1621 cctgaccgat gaaatccaaa aagcggcgac tctgatttca atacaggaaa atgccgttcc
     1681 tgttatgctc gaagaaaaaa tatttatgct caaaccggat gaaatcttta tggcacgggt
     1741 ggaaaataat tcgctggcaa tatacagtga atcaagaagg tatctctgta aaaaaagatt
     1801 atatgagatt tctgaaattc tcggcgccgg ttttatgcag atttcaaaat cgacggtcgt
     1861 aaatctttca gaggtggatt atatcgaacc ttttttcaac gggatggtga atctcaagtt
     1921 gaaaaacggt ctatccgact atatctcaag aaaatatatg aaagatttca agagatattt
     1981 tggtttataa aaggagaaga taatgataga aattataaag cgaatcatcg aggggatagt
     2041 aataggctgc accatcttta caataatcgg gatcattttt tgcctggtca acggaacgga
     2101 taccgcattc ggtgatcctg attatggatt tgtaaaaatg gcaatatgct caatggttgt
     2161 aggattgggt ttttctctcc caaccttcat ctatgatgat caaaaaatca caaaaccgtt
     2221 gcaaacgctt atccatttat caattggttt tatcattttc ggtttggtgg ctttctatgc
     2281 aaaatgggta ccggtcgaga gcggaattaa ggaaatcgtc gtttttatca tcctatcaat
     2341 tttgtcgacc ttggcgattt ggtactgctt ctatttgtat tacaaaaatg aagcgagaaa
     2401 aattaatgac gatatccgaa aaaaacaaaa caaaaattga acaaattttt gaacctcctt
     2461 ttcaatggat gacagaatcg ccggcacctt atcggaatga tagtcttgct gaataacaac
     2521 acaacccgtc atagccacaa ttgacccgtt tccaattatt tggtagaata ctgttcgccg
     2581 atgttgtacc cttcatcgga aaatatatct aaaataagga agggcttgaa aatgaaagta
     2641 ttattgttaa aagatgtcta taaactcgga catgcgggtg atgttaagaa agttgcggac
     2701 ggatacgcgc gcaactattt gattccacaa ggtcttgtta cgatggcgac tcccggtgcc
     2761 attaaacagg cggatcaaat caaacaaaaa gcggatgcga ttcgttctca actgaatacc
     2821 gaaatgagtt ctttattcga aaagatctgg gatggttata acttttgcca gcaaggccgg
     2881 agaaaccggc aaattatacg gatcaatcac tccgcaaatg atcgttgatg cgatcaatga
     2941 gaaatccggc gcctcaatcg aaaagcggca gttggaagtt gaaccgattc gaaccttggg
     3001 ggaacaccat gttaccgttc gattgactgt tgatattgtt ccgaccgtta aagtgattgt
     3061 tcatcgtgag ggtgaaaagg tcaatctgga atctcttgtg accgaagaac ctgaaaaagc
     3121 agaataaatt actattttat atacaatcgg aagaaggtca ggcatgtact ggcctttttc
     3181 ttttataatt acccaatatg tctgtgtctg tcggcaaagc gctacgatcg gctcgcgaga
     3241 gcaagaatat cccactcagc aaagtgtcgg atgatcttta tatccggatc acttatctcc
     3301 aagccataga aaacggacgt ccggaaattc ttccttcacc cgtacagggt cggggatttg
     3361 ttcggatgta ttccgattat ttaaagttgg atacggaaaa aatcctctat gcttgggatc
     3421 atcccgatat accgttttca cttgattctg agatgtcgaa tcaaaccgaa tcgcccgctc
     3481 ctcaggaacc gatcatagaa aaaacggaaa gttcaacgca accatccaaa tcggataccg
     3541 ccaacagtca aaacgcggac ccggtttctg ctatttataa acaaatcggc agcgaactgc
     3601 gacaacgcag agaaatcctc ggtttatcct atgaagatgc agagaaacat acacttgtcc
     3661 gcagtattta tcttaaatta atggaggacg gtaatttcga ttctttgcct tcttcggttc
     3721 aggcacgagg gatgctgaat aattatgcag ccttccttaa tctggatgtc gaaaacatca
     3781 tgcttcaata tgccaatgcg ctgcaattaa agtctgcttc aagaattgcc gaaaccgcaa
     3841 aagaaaaaaa gaataaaaag gagcctaaac cgacgaagaa agccggaaaa ttcaggcaat
     3901 tcatgacacc ggatctgttt gtcggtgtat cggttctgat cgcgatgttt gcaattatca
     3961 tttattccgc tgtgacaatt tcggaatata aagccaaagc cgttcagaaa actgtcgatt
     4021 taaatctgaa ttttatcaat caattggcaa ctcacgacat cacagtaacc gcgacgagcg
     4081 attttaatga aactcccacc gttccgataa gcgaaatgaa tccgtcggaa ccgacacaga
     4141 tgaataccca atcagaaaat ttgactgtcg aggagacaaa ccggcctatt caagtttttg
     4201 tggaagcgaa tcagagagcg ttcatgaaag tcgtttccga tggtgatgtg gtgtttaccg
     4261 gcagaactct gccggggaat acctatccgt ttgatgcaag tcaaatgatt gaagttacga
     4321 ccgggaatgc cgctgcgcta aaaataactt acaaccaaca atctttaggt ttgatgggga
     4381 aattaggtga ggtgataaca ctccagttca caaataatgc aattatgacc cccactcccg
     4441 cgatcaccat gacgccgacc cttacgttag aaccgacata cacacctcag gtattcgaag
     4501 caacaccgac ttttaccatt acaccttaca tcccttaaaa ttcaaggact tatgcaaaaa
     4561 aacacttttc atctaatttc cctcggctgt gcaaaaaaca cggtcgattc caataccgta
     4621 gccaatatat tacagaatgc gggctattca tttaatatcg atcccaaaca gtccgaatat
     4681 ctgattgtta atacctgcgg gtttattcaa tccgcccgtg acgaagccgt cgaaacgtta
     4741 caaggattgg cagccgacaa aaatccgggg caattcctat tggcgatcgg ttgtatggct
     4801 gaactttaca gccggcaaat tttggaagct tgtcccgaag tagatggtgt tctgggaacc
     4861 agaaaaatca ctgacatcct tcagctgata accggtgtca aagagacgga tattcaactt
     4921 aatccggttc ccgaagaaat tttgacgagc caatttacga ttcagggacc cgccgcatat
     4981 ctgaaaatcg ccgacggatg cggcaggtat tgctctttct gcagtattcc gttgatcaaa
     5041 ggcagttgga tcagccgtcc gcaagcagac atcctggctg atgcggttcg acttcaggaa
     5101 atgggaataa aagagttgat tttgatcgct caagacacta ccgcttatgg gattgatcgc
     5161 ggtgaaaaag atgggctgcc ggatttaatc gaaaagatcc tttcggcagc tccggatatt
     5221 ccgcggatcc gtgtccttta tgcatttccg ggattcgttt ccgatcggtt aatcgatctt
     5281 ctttcaaaca atgagagggt cttaccttat ttggatatac cgctgcaaca tgcacatccg
     5341 gaagtcctca aaagaatgca ccgccctgca gatatggatt gggtcaggaa gaccatcgca
     5401 aaaatgcgtt ccgctaatcc ggattttgcc atccgctcaa cctttattgt gggctttccg
     5461 aacgaaaccg aagaagagtt cgagacactt tatgactttc tgaatgagat gaaattcgat
     5521 cgtgtcggag ttttttcgta ttctttcgaa gaagggaccg cgagcgcggt ttacggagac
     5581 acggttcccg aagaagtcaa acgggaacgg ctcgatcgct tgatgaaact gcaacagggg
     5641 atttcaagag aaattaatcg gaaatttatc gggagaaaaa tggatgttct cgttgagagg
     5701 caacaagacg gattgctgat cgggcgaagt tatcgtgatg ctccggatat tgacgggttg
     5761 gtttttgttg aagggaaagc aaaaatcggt gatattatca aagtcagaat tcatacggct
     5821 ttggaatatg atttgatcgg gaaacgaatt ggttaaagtt aatagaacat gcgttttccc
     5881 ttcggaaaat cttctatgaa aatcagcata taaaacgaaa aagaatcaaa aatgcctcaa
     5941 gattttagag gcatttttga ttctttttcc cgaattctat taactcgctt tacggtaccg
     6001 tttcgggttc aggaatcggt tcggttgagt tatcaaccgg aggaatctcg acctgacccg
     6061 attcgccgcc ggacatcccc ggaacagtcg gagtgaacac catttcggtc ggttgttgga
     6121 tttgtacttc cggcgtcgca gtcggaggat aacattccag atatgcagca gtagctgtgg
     6181 gcgcatattg ttcatttgca ctcatagaaa atgcttgatt gtagtattca atcgaagcgc
     6241 aataatcgcc tgtttgagcc aatcggttgg catattcaaa agaagcctga atataacgtt
     6301 cctgtgcggt atatcccgaa gcgcgatcat acattccggg tgccgtcaaa taagcattgc
     6361 tgtaatactc aagcgctttt gcccaatcga tttcccagaa gccagcccct gccaaataac
     6421 tgcgcgccgc atcccgttga ttgcttgcca atgcatcaat cggtccgaaa gcttccgcta
     6481 aagtgatctt ataaatcccg gactccaagt aaccgttatt aatcaactgg atcccgtaat
     6541 tcctgagagc aatataatac atcccatcga cttcgataga tttgtaactc aaatttttat
     6601 ttcgaagagc ttcaatattc gctatggcag tctcccattg ttgagcagcc atatttgccc
     6661 gaatcgtgtt caacaattcc tcttcccctc tggtatccgg agtagaagtc ggggcgggag
     6721 tcggagtata aaaaggtgtg ggagtctctt tgggataaag cgcaaccacc acttcggtgt
     6781 atttttctgt cgcaccggga taattcggat cgacacttag tacatattcc aaccgggttt
     6841 tcgcattttc gtattttccg ttttgaatat ccgcgatggc taaattatat tgctgtgccg
     6901 ccgctttaat tgattgctct ttataagaat tttgccgtgc ttgaattccc gtttgataac
     6961 cgatcacccc tcctgcggct attaatacca acatgatcag aatgccaagc caaacccatt
     7021 ttccgtttcc ctgctttttc ggcttcgcag ctttcttttg atttttcttg ggctgtaatt
     7081 gaggattctt attccccggc atcgaaggtt gcccttgagg atattgcgga ggcggataat
     7141 atccttgctg ttgaggataa taactgcgcg atgggtcata tggagtaggc tcaccttgcg
     7201 gtatatgact tccgtagcta ccgctgtaat tttgagagga cggattcggc gtattttccg
     7261 aattcttcgg tgtttttgga ttcttatcac tgtttgattt gttttgattc attttttact
     7321 ccggcgatca gatatcgcta tctatctgca caaacttctt tcttcgtaaa acttttctaa
     7381 aaatgacgca aggctttaat ttcaggcaaa atgaaaatca aatagacgag aaaagctgcg
     7441 cccattccaa taatagattt taccagatta tcagaaaaca ccggattata atgcatcgct
     7501 tcataggtaa tgattccggc tattcccgca ccgatggcag atcgtatgat cggattccaa
     7561 agctgcggag ctgcattcat ttgaggaaca cttggcgcgc tttgttttcc ttttttccca
     7621 taaaaccgat tatttctctc agcgggttcc atccgtaaca gaagcagaag aaaaagagct
     7681 tgcaatgaaa acgagacgga atccgtcaaa ctgatcccgg gagcattcaa agggcgatac
     7741 aggatcctgc ctaagatgat atatattaca aggttcaaaa tcgttccgat caaagggatt
     7801 cgtgcatttt gttgggcgta gaaagcccga ttgaccactt caagcaaaca atggccggtc
     7861 agtcccacca aaaatccccg caaagtccac atcagcagag cattttccgc aacgttaaat
     7921 cctaaaaccc gttgaacgat cggacctaat ccgacgccca taatgactgc tataccgatt
     7981 gtgagcgcaa cggataagcg gcaggcaatt tcaaaagtct tctgaaattc cgctttttta
     8041 ttttccgaaa taaataagga aagcgacggt aagattgcgg ttcccaaagc cgtcccgatc
     8101 agtgtctccg gtaattgttg aaacatgtag ccgtagctta aagccgttac cgatccggtt
     8161 tccaaccgag atgccaagtt atcccgaata ataaaaatca attggacgaa aaaaacggtt
     8221 aagatcctcg ggatcatgat ccgaatggtt ttttgaacat acggatcttt ccattgaatc
     8281 atgggatacc atttaaattt taatcgaatc agaccgggga tttgaatcag taagtgaagg
     8341 atagccccga ggataacccc gccggctaag ccgtagattc ccattccaaa agcgggaagc
     8401 tcgatttgtc cgatcctgta tggttcttga ggagaaagaa ccaccgcgcc aaatatttga
     8461 ccgatattat agagtatggg tgctaacgca gaaaggaaaa attttcgatt cgcttgcagc
     8521 ccgcccataa ccaatccgct gatcgagaaa atcagtgtcg caattaaatt caggcgcatc
     8581 atatttacca catgttcttg ttgttcggtt gtaaaacccg gagcgattcc tatctccgaa
     8641 gtgacgatag gacgtgcaaa tatcgcgata aatatcgcta aaatcaatga aaccacgaag
     8701 gcaagattgg cgatggatga aaagactctc caagacgctt cccgactttc cttctgaata
     8761 atttctgtca taatcggaat aatcaccatt gccatggcgc ctccgctgat cagaataaat
     8821 aacatatcgg ggagattatt tgcaatgtta aaaacgtcca attccgcaga aagtccaaat
     8881 tggcgggcga taattaattg ccggataatg gctatgacct tatcgatccc gaaaaagata
     8941 acaatcatca aggaagttct tgaaagggaa gataacttca tagttccatt ctatcattca
     9001 agatttatga cagcatgaca aaatatgttc cggatcataa tttctcccaa aaaaatcctg
     9061 atagaattca gctatgagaa taaataacgc cctttatgtt gtggcaacg
//
LOCUS       DAOMIV010000014         9096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_7701_length_9096_cov_5.118682, whole genome shotgun sequence.
ACCESSION   DAOMIV010000014 DAOMIV010000000
VERSION     DAOMIV010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7701_length_9096_cov_5.118682"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1731
                     /gene="murJ"
                     /locus_tag="PKV59_00760"
     CDS             163..1731
                     /gene="murJ"
                     /locus_tag="PKV59_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQP45309.1"
                     /translation="MEQTSEPKQTFMKSATILAGAMILSQIIGLISKGLMGAAFGASA
                     EVDAFLASNRLTETLFNLVAGGALASAFVPWFSSLLDQKDEQSAWKLASDIANLLTII
                     LLLISVACFIFAPQIVRYFLVPGFSLNDPHLQDLTVSLLRIQLPSVIIFGLSGLVMGI
                     LNTHHHFLVPGLAPAMYQLGIIFGVVFLKKPLGIKGLALGAVIGSFLHLLVQIPQLLS
                     LEGKHYSFSLGLRDYRVRNVIKLMIPRQIGASAVQLNFLVNNFIASFLPAGSISAVTW
                     GLSLMLMPQAAIAQSVATVSLPMFSVQAARGELDEMRNALASMLRFVILLALPAACGL
                     ILLGKPLVSLIFERRAFNSEMTAMVYWALAWYSSGLIFHCVVEVISRAFYAVHDTKTP
                     VLVLLIAMLLNMGLSILLSRLFRSLGWMPHGGLALANTIATGLEMTTLLTLMRRKLHG
                     LEGKRILQALMQSAVSATLMVIVLEVWIRFSVGSSDLIIVIVGVILGVSVYAGMCFIF
                     KVEELSSIRKLIHI"
     assembly_gap    1902..2001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2002..3068)
                     /locus_tag="PKV59_00765"
     CDS             complement(<2002..3068)
                     /locus_tag="PKV59_00765"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HQP45310.1"
                     /translation="MTENQSKKKVIIIGAGPAGLTAAFELLRQEPESYNVTVLEESAA
                     IGGISRTVQYNGNRMDIGGHRFFSKDQRVMDWWKERMPIQGSPSKDDILTHTVKPLDP
                     KGPDPEKEDIVMLFRNRVSRIYYLKKFFDYPISLKAQTFINMGLKNTLISGSSYLVSV
                     FHKLPETSLENFYRNRFGKKLYSMFFENYTEKVWGRHPSEISADWGAQRVKGLSVTKI
                     IIDMFSKILPAPLKKNRPVETSLIEQFWYPKFGPGQLWELTADQITQMGGIIRKNCQV
                     TGIKLVNNQVQAVTVIENGEQKEIEGDIFLSSMPLKDLVMGMGSAVPDEIARIAKGLP
                     YRDFITVGLILSDLKLKNQTK"
     gene            complement(3188..5569)
                     /locus_tag="PKV59_00770"
     CDS             complement(3188..5569)
                     /locus_tag="PKV59_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQP45311.1"
                     /translation="MDLIGQKYRIIEECGEESENSFYRVVDETDGKHYYLDLIRTDNP
                     SLSAKGLIELQVRAQEHSRFRHSNAIPFYPPEVTDNGLILIQNDIPGKSLNYVMKTRG
                     RPLPQNQAYRITYAVVSALAAMHDLQMTHGELDSGHILINDSGDSFISFLPLPDDFNT
                     QNALYRFPETESTVKSKFSDDIYAMGVILTEMFTSLIPFGFAENKDGKKNTRAFEYYK
                     IGLAQISDESNEAIIPVILRCLNPDPKNRYENAVELFIDIRKIIEAWVNNTPGQENIL
                     VLPVIKKPIDQKEEDFENKIKKPSSSSKERVRSQRKSNKKKGSLLISFISAAIVITGG
                     IVLFFYLSEVADTKTSVTNYQGTLENLNNTQTALAYNPAIITPQVTKKSSSSIKKTAI
                     PTPLPTFTLTATATFTPVPIQRSIGAAIHWTADQSEMVFVPSGNFRMGMDATFGFAIE
                     NLFPVHEVDLDNYWIDRTEVTFEQYSQCVKAGACPASEAASPITADQKKPVTGISWEN
                     AATYCKWAGKRLPTEAEWEKAARGTDLRLYPWGNDSPQYTDQGNWFSTEIQNADEMNR
                     DISPYGAIHMSDNVSEWVNDYFSPTFTTATEYQNPQGPVSGAMRTVKGGAATNQDPEI
                     ASFVFDRWSADPIRPQDYGFRCAVSDSDQIQEKANGSEKPVPVSPLGTIPETGCENRI
                     GFVADITIPDGTPVHANEIITKTWRLKNIGTCTITKNYKIVWADTTFVNPQKLFDFNT
                     EILPGEEKEVSITFPVIGNGKTRINFKFAGLDGVPFQLGERGRGAFWIEYVIQ"
     gene            5863..6339
                     /locus_tag="PKV59_00775"
     CDS             5863..6339
                     /locus_tag="PKV59_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45312.1"
                     /translation="MADIEKIIKEVQEAIHSGSEKVTDSVTESDMFDKYKDSDDKIGD
                     ILEDAGNVVTGWFDAVSDKVEPVVKKTKAKIYEGIQEEYRKAGAPYGDTHEGLLRWVD
                     EHETNIREKVNTGLEKARKTILDTAKKTKDFVEEKITENQSASKTTVEGSTKKSEK"
     gene            complement(6476..6832)
                     /locus_tag="PKV59_00780"
     CDS             complement(6476..6832)
                     /locus_tag="PKV59_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45313.1"
                     /translation="MTAKSLYMNNFTFSLQNLTCILDAKSVAPDIERSNFGTVLDEKK
                     TVDEVFLFMPIEAAKELGISILQMVAAQEKRTHLKIRLEPEKQALWDCYVNAAEEFRQ
                     QNNEAKKADEKTTPSV"
     gene            complement(6829..7311)
                     /locus_tag="PKV59_00785"
     CDS             complement(6829..7311)
                     /locus_tag="PKV59_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQP45314.1"
                     /translation="MMKSNNLFRLNVGFLINQAVGSYRQFEIDAPFIQIDEDTRFNNV
                     KTDIRVSRVQQGILVSVEAGAVIDLECVRCLDPYVQTIDCNFDELYAFHIRQNIGAES
                     YLPENGFIDLLPLLEEYLTLEIPISPLCDENCKGLCDVCGANLNYETCIHHQKLEIKS
                     "
     gene            7411..>9096
                     /locus_tag="PKV59_00790"
     CDS             7411..>9096
                     /locus_tag="PKV59_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45315.1"
                     /translation="MIRPPHDLSIPRKKTTVFILILSFLEGVAVFVHLISIPSESENA
                     FLLGFSKSRLLLIVSCIFLSIFYAFLLIRFLRKGFLLPDRRKNIWLYRILISIFWVIF
                     WGIFFFLLLSVLPVQKSDSTVSSIAFRIWDVLLWGALLSVQMIFLIQFQTGHWHEIFN
                     NYIKAFFFALFLIVFYLFISYYDRIDWIRKFKYSEITILTPAGIALIWAILSGKIKDR
                     THAEKLDTIFLCLLIGSLTYSIYRTINCFMGQLGTPLYSYWDLLADAFNHGHLDLTAP
                     PTTHDLTLYNGKWYVPNPPLPAILLMPVVALLGVEGVNMTIVSALLGSLNAVIVYLVL
                     RYAAESGMTACKRSANLWLTAVFALSTNHIWLATTGQMWFISQLVTILFIGLGTIAVI
                     RGKPAWVSGLMIGMAVMTRPNIFPIAIFLMGIYLWKAKPYPEVEWGKAVRWAIGFGIP
                     VLICVFMELLYNYLRFEDWFDFGYVTINGAESIVEAVQKYGMFHPHFLRENFEVMFLR
                     MPRIDTTGERFFFQPGYAGYSIFAMTPAFIYLIRGIKKNWWVIGAWVSVILTVG"
BASE COUNT         2290 a         1813 c         2022 g         2871 t
ORIGIN      
        1 acaaattact aaaggatttt tcataaactt taccgtttct tagctctttt tttgatgggc
       61 taattataaa gtcatctcgt tcattaagat gtgagttatg agagagttca agccggaata
      121 aacttttcga gagttcttcc cttcatgata gaatcaattg cgatggaaca aacttcagaa
      181 cccaaacaaa catttatgaa atcggcgaca atactggcgg gggcgatgat cctctctcag
      241 ataatcggct tgatttccaa aggtttgatg ggagccgcat tcggggcttc cgccgaagtg
      301 gatgcttttt tggcttccaa tcgtcttacg gaaacgcttt tcaatctcgt tgccggggga
      361 gctttggcaa gtgcttttgt cccttggttc agctctttgc tcgatcagaa agacgaacaa
      421 agtgcttgga aattagcttc cgatatcgca aatctgctga caattatcct gttgttgatc
      481 agtgttgcct gttttatttt tgctcctcag attgtgcgtt attttttggt tccgggtttt
      541 tcattaaatg atccgcatct gcaagattta accgtctcgc tgcttcgaat tcaattaccc
      601 tcagtcatca ttttcggctt gagcggattg gtgatgggaa tattgaatac ccatcatcat
      661 tttctcgtgc cgggcttagc tcccgcaatg taccagctcg gaattatttt cggagtcgtc
      721 tttctgaaaa aaccgttggg aattaaggga ttggcgttgg gggcggttat cggctctttc
      781 cttcatcttc tcgttcaaat tccccaatta ttgtcgctgg aagggaaaca ctattctttt
      841 tcattgggtt tgagagacta tcgggttcgc aatgtcatca aactgatgat ccctcgccag
      901 atcggtgcct ccgcagtcca actgaatttt ttggttaaca atttcattgc ttcgtttttg
      961 ccggccggat cgatttccgc ggtcacgtgg ggactttctc tgatgcttat gcctcaggcg
     1021 gcaatcgctc aatccgttgc gacggtatcg ctacccatgt tttcggtgca agcggcgaga
     1081 ggagaattgg atgagatgcg caatgcgctg gcttcgatgt tacgatttgt cattttgttg
     1141 gcgttgccag ccgcttgcgg attgatttta ttgggcaaac cgctggtcag tctgatcttc
     1201 gaacgccggg cttttaattc cgagatgacc gcgatggtat attgggcgtt ggcttggtat
     1261 agttccggct tgatctttca ttgtgttgtc gaagtaattt cccgggcttt ttatgccgtt
     1321 catgatacaa aaacgcccgt tttggtcctt ttaattgcca tgttgctgaa tatgggactc
     1381 agcatcctgc tgtctcgttt gtttcgaagt ttaggctgga tgcctcatgg tgggttggct
     1441 ttggctaata cgattgcgac gggattggag atgacgactc tgttgactct gatgcggcga
     1501 aaattgcatg gtttggaagg aaaacgaatc ttgcaggcgt taatgcagag cgccgtctct
     1561 gccactttaa tggtcatcgt tttggaagtt tggatacgat tcagcgtcgg gtcttccgac
     1621 ttgattattg taatcgtcgg agtaattctt ggtgtatcgg tttatgcggg aatgtgtttc
     1681 attttcaaag tggaagaatt atcttccatt cgaaaactga ttcatattta acctttcatt
     1741 tctgctcagg ttgtttcgtg ataaaccttt tcggtattta cattccagat attttctctg
     1801 gatcggatat ttccgcgaat atttttaacc gcttcgaacg ccgttgccat cgaatgatcg
     1861 gaattattat agcgatgctg accgtttcgt ccgatgcaat annnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nattttcgtt tggtttttca gtttcagatc cgacagaatc
     2041 aaaccgacgg taataaagtc ccggtaaggc aatccctttg cgattctcgc tatttcatcg
     2101 ggaactgccg atcccattcc catgaccaaa tcttttagcg gcatactgga gagaaaaata
     2161 tcgccttcga tttccttttg ttctccgttt tcaataaccg tcaccgcttg tacttgattg
     2221 tttacgagtt tgataccggt tacttggcaa ttttttcgga tgatacctcc catttgagtg
     2281 atctgatccg ctgtaagttc ccaaagttgg ccgggtccaa atttcggata ccagaattgt
     2341 tcgatcagtg aagtctcaac cggccggttc tttttcaagg gagccggcaa aatttttgaa
     2401 aacatatcga tgataatttt tgtgacggac aatcctttaa cccgttgagc cccccaatcg
     2461 gcggagattt ccgaaggatg cctgccccag actttttccg tgtaattttc aaagaacatc
     2521 gaatacaatt tttttccgaa ccggttacgg taaaagtttt cgagtgaagt ttccggtaat
     2581 ttatggaaaa cggaaaccaa ataactggaa ccggatatca gcgtattttt taaccccata
     2641 ttgataaaag tttgggcttt taatgaaatc ggatagtcaa aaaatttctt cagataataa
     2701 attctggaaa cgcgattgcg aaaaagcatg acgatatctt ctttttccgg atccggtcct
     2761 ttgggatcca acggctttac ggtgtgagtc agaatatcat ccttggaagg gcttccctgt
     2821 attggcattc gttctttcca ccaatccatc acccgttgat ctttcgagaa aaaacgatgt
     2881 cccccgatat ccatacgatt accattatat tgaacggttc tggaaatgcc gccgatggca
     2941 gcggattctt ccaaaacggt aacgttataa gattccggtt cctgtctgag aagttcaaat
     3001 gcggctgtca atcctgccgg gcctgcgccg ataataatta cttttttctt tgattggttt
     3061 tctgtcatat cgcttccgga tttatttttt gatctcgtca ttgttcgatc agtcattcga
     3121 caatgcgatt ataactgatc tgagtcgtta aaaaggaatc ggatctcttg ggagaatcga
     3181 ttctgtttta ttggatcaca tattcgatcc agaatgcgcc gcgacctctc tcacccagtt
     3241 ggaaaggaac accgtccaac ccggcaaatt taaaattaat tcgggttttt ccgttaccaa
     3301 tgacggggaa agttatgctt acttcctttt cttccccggg aagaatttcg gtattgaaat
     3361 caaataattt ttggggattc acaaaagttg tatccgccca gacaattttg taattttttg
     3421 taatcgtaca ggtgccgata ttctttaacc gccacgtttt tgtgatgatt tcatttgcat
     3481 gaacaggcgt tccgtccgga attgttatat cggctacaaa accgattcga ttttcacaac
     3541 cggtttccgg aattgttccg agaggtgaaa ccgggaccgg cttttccgaa ccgttggcct
     3601 tctcttgaat ttgatcggaa tcgctgacag cacaccggaa tccataatcc tgtggacgga
     3661 tcggatcggc actccaacgg tcgaaaacaa acgaagcgat ttcaggatct tgattggtcg
     3721 ccgcccctcc tttgaccgtt cgcatagctc cgctgaccgg tccttgcggg ttttgatatt
     3781 cggttgccgt tgtgaatgtc ggactgaaat aatcatttac ccattcgctg acattatcac
     3841 tcatatggat tgctccataa gggctgatgt ctcgattcat ttcatctgca ttttggattt
     3901 cagtggagaa ccaatttccc tgatcggtat attgagggct gtcgtttccc caaggataaa
     3961 gccttaaatc tgtaccgcgc gccgcttttt cccattccgc ctcggtcgga agacgcttgc
     4021 cggcccattt acaataagtc gccgcatttt cccaagaaat accggttaca ggtttttttt
     4081 gatcggctgt tatcggactg gcagcttccg aagccgggca cgctcctgct ttaacgcatt
     4141 ggctgtattg ttcaaatgtc acctccgttc gatcgatcca ataattgtcc agatcaactt
     4201 catgcaccgg aaaaagattt tcgatcgcga acccgaaagt agcatccata cccatccgga
     4261 aattccccga aggcacaaat accatctcgg attgatcggc agtccaatga atggcggctc
     4321 cgatggacct ttgaatcgga accggagtga aagtcgcggt cgccgttaat gtaaatgtcg
     4381 gcagaggggt agggatagct gtctttttta tgctcgaaga agattttttc gtcacttgag
     4441 gagtaatgat tgcggggtta taagccagag ctgtctgtgt attatttaag ttttcaagag
     4501 ttccctgata gttggtcacg gaggttttgg tatctgccac ttccgaaaga tagaagaaca
     4561 gaacaattcc gccggtaatt acaatcgcag ctgaaataaa agaaatcaac aacgagccct
     4621 tcttcttatt gcttttgcgc tgggatcgga ctctttcttt ggaagaagat gacggttttt
     4681 ttattttgtt ttcaaaatcc tcttcttttt gatcgatcgg tttctttatg acaggcagta
     4741 ccaagatgtt ctcttgtccg ggtgtattat tcacccacgc ttcgatgatt tttcgaatat
     4801 caatgaacaa ctccactgca ttttcatatc gatttttcgg atcgggattc agacagcgta
     4861 aaatcaccgg gataattgct tcgttcgatt catcgctgat ttgcgccagc cctattttgt
     4921 agtattcaaa tgctcgggta ttctttttac cgtctttatt ttcagcaaaa ccaaagggga
     4981 ttaacgaagt aaacatttct gtaagaatca cacccatagc atagatatca tcactgaact
     5041 tggacttgac ggtggactcc gtctcgggaa aacgatataa agcattttgg gtgttaaaat
     5101 catctggaag aggaagaaat gatataaaag aatcaccgga atcatttatc aaaatatgac
     5161 cggaatcaag ttccccatga gtcatctgaa gatcatgcat agctgccaat gccgaaacaa
     5221 ccgcataagt aatacgatat gcctgatttt gcggcaaagg acggccacgg gttttcataa
     5281 cataatttaa tgatttcccc ggaatatcat tttggataag aattaatccg ttgtccgtaa
     5341 cttcaggagg ataaaatgga atcgcattgg aatgtctaaa gcgagaatgt tcctgtgcgc
     5401 gaacctgtag ttcaattaat ccttttgcac tgagagatgg gttgtccgtt cgaattaaat
     5461 ccagatagta gtgttttcca tctgtctcgt ctacaactcg ataaaaggaa ttttcagatt
     5521 cttcgccaca ttcttcaatg attcggtatt tttgtccgat gagatccata tcaaatccga
     5581 ttgtcgataa ttttcattat cactttgaat caaattatag tttattgaac gcgattaata
     5641 gcattcgttt tgttgttttg taagatagaa aattattgaa taatcaaatc gttaaaaagc
     5701 atgtgagagg tttattttga ttgaaccgaa tggttcaaaa gttctgcttt tttggaccaa
     5761 tcatatctcg agacattttc tcatagatgc gctatcatat actttgaaac tattcggata
     5821 ttatttcgta tacgttttat cggaatttat aaagaggaaa tcatggcgga cattgagaaa
     5881 attattaaag aagttcagga ggccattcat tcgggttctg aaaaagtcac cgattcggta
     5941 acggagagcg atatgtttga taagtataaa gattcagatg ataagatcgg ggatatctta
     6001 gaagatgcgg gaaatgtcgt caccggttgg ttcgatgccg tatcggataa agtggaaccg
     6061 gttgtgaaga agaccaaggc aaaaatttat gaaggtattc aggaagaata ccgaaaagcc
     6121 ggtgcgccat atggtgatac acatgaaggt ttgctgcgtt gggtggatga acatgaaacc
     6181 aacatccgtg aaaaagtaaa taccgggctt gaaaaggcac gtaaaactat tcttgatacc
     6241 gcaaagaaaa caaaagattt tgtggaagaa aagatcaccg aaaatcagtc cgcatctaag
     6301 accacggtgg aaggaagcac aaaaaaatcg gaaaaatgat tctgatttat tttaaactta
     6361 gcggaaatgg ataggtttta aacaagattc tcaataattc aatgggcgat ctcattccgg
     6421 aaaattcact ccggtgagat cgctttttta tttcgttttc tgaagcttca ttgatttaaa
     6481 cagagggagt ggttttttca tccgcttttt tggcctcatt gttctgttgc cggaactctt
     6541 cggctgcgtt tacatagcaa tcccagagag cttgtttttc cggttcaagc ctgattttta
     6601 aatgtgtccg tttttcttgt gctgcgacca tttgcaaaat cgaaatcccc agttcttttg
     6661 ctgcttcgat cggcatgaat aagaatactt catccaccgt tttcttttca tccagtaccg
     6721 taccgaaatt ggatcgttcg atgtcgggtg cgactgattt tgcgtccaaa atacaagtca
     6781 gattttgaag actgaacgta aagttattca tatacagact ttttgcagtc atgatttaat
     6841 ctccaatttt tgatgatgga tacaagtctc gtagttgaga ttcgcaccgc aaacatcaca
     6901 aagcccttta caattttcat cgcaaagagg actgatgggg atttcgaggg tgagatattc
     6961 ctctaaaagg ggtaaaagat cgataaatcc attctctggt aaataggatt ccgcaccgat
     7021 attttgccga atatgaaatg cataaagttc atcaaaatta caatcgatgg tttgtacgta
     7081 tggatccaga catcggacgc attccaaatc tattacggct ccggcttcca cggaaaccag
     7141 aattccctgc tgtacacgac tgacacggat atctgttttt acattgttaa atcgggtgtc
     7201 ctcatcgatt tgaatgaaag gtgcatcaat ttcaaactgc cggtaagagc ctaccgcttg
     7261 gttaattaaa aaaccgacat ttaagcggaa aagattattt gatttcatca taatattttt
     7321 ccaattttca tgctgattat atcgcatatc gaaaattgtt ctccgcctcg ttacgagaat
     7381 ttcaatagga agcgtgatat aaatatccgt atgattcgcc cacctcacga tttatcaatc
     7441 ccccgaaaaa aaaccaccgt ttttatcctg attctttcct ttttggaagg ggttgcggta
     7501 tttgttcatt tgatttcaat tccatccgaa tccgaaaatg ctttcctttt gggattttca
     7561 aaaagtcgtt tgctcctgat tgtgagttgt atttttttaa gcatctttta tgcatttctg
     7621 ttaatccgat tcctgcgaaa gggatttttg cttcccgacc gtcgcaagaa tatttggttg
     7681 tatcgaattt tgatttcaat tttttgggta atcttctggg gtattttttt ctttttactg
     7741 ttgtctgttt tgcctgttca aaaatcggat tccactgttt catcgattgc tttcagaatt
     7801 tgggatgtac ttttatgggg ggcactgctt tccgtacaga tgattttttt gattcaattt
     7861 caaacgggtc attggcatga aatatttaat aattatatta aagcgttttt ctttgcgctt
     7921 tttttgattg ttttttattt atttatttca tattatgatc gaattgattg gattcgaaaa
     7981 ttcaagtatt cggaaataac aattcttaca ccggcaggta tcgctttgat ttgggcaata
     8041 ctgtccggaa agatcaaaga tcgtacacat gccgaaaagt tagacaccat ttttctttgc
     8101 cttctcatag gtagtcttac ttattcgatt tatcgaacaa taaattgttt tatgggtcag
     8161 cttggcactc ctttatattc ctattgggat ttgttggcag acgcctttaa tcacgggcat
     8221 ctggatctga cggctccgcc gacgacacac gatctgacat tgtataacgg gaaatggtat
     8281 gtaccgaacc cgccgctgcc ggcgatcctg ttgatgccgg ttgttgccct gttaggggta
     8341 gaaggcgtga acatgacgat tgtctcggcg ctgttggggt cactgaatgc ggtaatcgtc
     8401 tatttggtat tgcggtatgc ggcggaaagc gggatgacag catgcaagcg atcggcgaat
     8461 ctatggttga cggcggtatt tgcgctgagc acgaatcaca tctggttagc gacgaccggg
     8521 cagatgtggt ttatcagtca attggtgacg atattgttta tcggattagg gacgattgcg
     8581 gtgatcaggg ggaaaccggc atgggtgagc gggctgatga tcgggatggc agtgatgacg
     8641 cgaccgaaca tatttccgat tgcgattttt ctgatgggga tctacctatg gaaagcgaag
     8701 ccgtatccgg aggttgagtg ggggaaagcg gtgcgatggg cgattgggtt tgggataccg
     8761 gtactgatct gtgtttttat ggaattgctc tacaactatc tgagatttga ggactggttt
     8821 gacttcggtt atgtcacgat caatggggcg gaatcgatag tggaagccgt tcaaaaatac
     8881 ggaatgtttc acccgcattt tttgcgagag aattttgaag taatgttttt acggatgccg
     8941 cggatagaca caacggggga acgatttttc tttcagccgg gatatgcggg gtacagcatc
     9001 tttgcgatga cgccggcatt catctacctg attcgaggga taaagaagaa ttggtgggtg
     9061 atcggggcct gggtatcggt catcctgacg gtaggg
//
LOCUS       DAOMIV010000015         8972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_7839_length_8972_cov_4.991365, whole genome shotgun sequence.
ACCESSION   DAOMIV010000015 DAOMIV010000000
VERSION     DAOMIV010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7839_length_8972_cov_4.991365"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKV59_00795"
     CDS             <1..714
                     /locus_tag="PKV59_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45316.1"
                     /translation="KLPDFKGIITDLSGPTANMYGFECPKKLAQGACKDKRCLYPQPC
                     LMLKIDHQPSLSLLNKARKIPGIKKVFVASGIRYDMILHDPGCGKSYTRELCNYHVSG
                     QLKVAPEHDSPKVLQLMGKPGKELLLQFKRDFDQINRDTGKKQYLSYYFIAAHPGCTI
                     KDMQGLKTFANEELHIAPEQTQIFTPTPSTWSTLMYATETNPFTGEHIFVEKDPNQKQ
                     RQKDILTQKEQKKTWTKHS"
     gene            696..1622
                     /locus_tag="PKV59_00800"
     CDS             696..1622
                     /locus_tag="PKV59_00800"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HQP45317.1"
                     /translation="MDKTLVIYNRYACKGKFKYSIQDVEKGLKAEEIIADMVLTEYPE
                     HAIDLVEKSVKEGYRQVIIVGGDGTINEAVNGLIQAQKKGYGKATLGVIQNGRGNDFA
                     FGMKIPNELKEAISLIKKAQPIPMDVGYVKTNEKSRYFCNGAGIGFDSAINYRATMSH
                     LTGFPSYFFGLIGSLIKDFKQYNAKISWADGETEMMILLLTTMNGTREGGGFLLAPDY
                     DFQDGKLNAVLIGNGKPLHQLLPLIPRFFTGNLDHPDILRIKTPWLKAEFSTTGLYGQ
                     ADGEIICSAGTEFYAEICPEKIDIISGLPFRK"
     gene            1667..2650
                     /locus_tag="PKV59_00805"
     CDS             1667..2650
                     /locus_tag="PKV59_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP45318.1"
                     /translation="MEKLKFGILGSAGIAWKQFIPAIALAKNAEIIAVASRSEEKSKD
                     FAEKYHIPLSFGSYEDLLHSKEIQAVYIPLPNRLHKEWAMKAFNAGKHVLCEKPLAIT
                     PEDCLEMGREAEKNHVLLMEAFMYRFHPLNEQIIRNVQSGILGGLETIQACHSFLLKD
                     PNNIRLNAELAGGSLMDVGCYCVNVTRTLFNEEPEAVAAVAHYHPNGVDDQMNILAKF
                     KSGRSAQLSCGLTSDYPSGYTISGRKGYIHAHYGFHLGVSNGTVTQKIDGIESILKDP
                     SNEYTRMIEHFSDCVLNQKPLRYTAAEAAGNMAFIQAALKSAENNGEWTEV"
     gene            2677..4692
                     /locus_tag="PKV59_00810"
     CDS             2677..4692
                     /locus_tag="PKV59_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45319.1"
                     /translation="MINRIRKRISDHPVRSFIVASGMIAFLAYGLLIPQLGMYGDDPN
                     FLWAYHRGGPNEYKEFMGWIREYGYLIYSFLSPVFGESIISWRIATLVLRWGSSILFF
                     LVFNNTFPNFQKIVVPLSISFLLYPGFMEQAIPVEFLLHFFSLDLILVSLLFTYYGIK
                     HGKGWRIFLFLSVLSEISGLFICEYFAGLELFRPIIIWQTIHQIPQISAQSVKNKIKK
                     TGLFAFPYLLIFGFFLYWRAFLSSSTYMSQIVTVRLQDNPAMFIGNLMRQILSDFKVA
                     VGDAWLQIFQIPSPGKIRWVYLAIFSLVGIVTWLLIVSTGKKEEKPSDQKLWEFLLIG
                     IFLFLTGGIPVWAAGLKISLTLYGDRLSLSFMAGACIIVMALLALILRQKYFPVLLAI
                     LIAFMAGYQFQLQNQFRKEWDLAKNYFWQLAWRAPDISPNTLVLGDQFPFRSLTDNSL
                     NALLNWNYESPEYPDRELYKFFQISAREGMIDPLPANSPLSHNDFRGNISDTILLYAT
                     PTTCLKVLTTADRNLPFLNIAMKKNLWRSKPERIIPNPTRTVTFQSFMGTEPFHGWCY
                     YFEKGELAAQQNQWKEVVEIGKEAESAGLIPSTMIEMKSFFFAALSENDLETAEHWKN
                     LILTEKGNANYFLYQIKEFQNQSHLSKEAELILDQIQEAFQRELESL"
     gene            4906..5904
                     /locus_tag="PKV59_00815"
     CDS             4906..5904
                     /locus_tag="PKV59_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP45320.1"
                     /translation="MEKIKFGILGTAGIAKLRFIPAIRNAKNAEVSAVASRNSEKSRR
                     YAEENGIETAYGSYEELLAQKDIDAVYIPQPNGLHKEWSLKAIAAGKHVLCEKPLAPT
                     PEDCLEMKQAAEAKNVVLMEAFMYRFHPLTEFVISQVHSGALGEIESIQASFTFILAD
                     MEDVRWDQKLAGGAVMDVGCYGINASRTFFAEEPTAVSAIAHYRKPGIDDRMGIFARF
                     PSGKQAQIICGFTSDAPIGYTINGTKGWITAYNGFQTGVLNGTITQEISGVRSTFRDS
                     TNEYTRMIEHFSDCVINHKPVRYDATDAARNMAFINAAQKSAENDGEWVKVKEIKS"
     gene            complement(5908..7125)
                     /locus_tag="PKV59_00820"
     CDS             complement(5908..7125)
                     /locus_tag="PKV59_00820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP45321.1"
                     /translation="MKILILADGRSPITLNWLRSLKSLNHQIVLVSSYPCEQPQEADE
                     FFILPVAFSGLSGSSIPKNKAKDGSKSEGSDRTFIRRFRPLLINFRYILGPFSLPLTA
                     RKFNRIVSQTNPDLIHALRIPYEGMLLSFYRGSIPCAVSIWGNDITLHAQGSAMMRQL
                     TQKTLKKVRGLAADTNRDIRLGENWGFPADEPILVVPGNGGIDFTKVRITAEKEQVGL
                     NWLPENWNIVINPRGFRPGSVRNDTFFQSIPLILRQFPNTLFLCCSMAGQPEADAWLD
                     ELQIHRNTMLLPTIPQELLWSLFSRAQVTVSISEHDGTPNSLLEAMTCGCFPVAGDIE
                     SIREWITPGVNGLLVDPNNPEQLASAVISALLNEKLRSDAAEINRQIILERADSEIAR
                     TEIEVFYHQCVSD"
     gene            complement(7173..>7679)
                     /locus_tag="PKV59_00825"
     CDS             complement(7173..>7679)
                     /locus_tag="PKV59_00825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP45322.1"
                     /translation="QERKRVDLLIEACAALKDSVSPILWIVGDGPARQQITELAAKIF
                     PQTLFFGALYGQELTEKFDQADLFVLPGTGGLALQQAMASGLPVIAAEADGTQTDLVR
                     EENGILVKPGDLKMLTDAIRTLLSDPSRLRKMGDRSFQIVQNEINLENMAESFEQAIS
                     YAVQNRKE"
     assembly_gap    7680..7779
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7780..8069)
                     /locus_tag="PKV59_00830"
     CDS             complement(<7780..8069)
                     /locus_tag="PKV59_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45323.1"
                     /translation="MKIGIVQRVLAEYRVPLFDLLAESYNGNVSVFAGDPRKDEMIDT
                     SRKLEKAHFYHANNKHLFHGKFYLCFQTNIFQWLRSQNPDVLIVEANNRYPLT"
     gene            complement(8066..>8972)
                     /locus_tag="PKV59_00835"
     CDS             complement(8066..>8972)
                     /locus_tag="PKV59_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HQP45324.1"
                     /translation="AHEQCREKESELALNENRWQEVQQAKAALQPIQREIIDLTNQIA
                     ADEKELKQIEGSVEQQKAALDEFSANLPDLQKAKKEFDTVQAETNRIRLEKGAAEQLV
                     SVLESLKKQKQEVIREREEANLQISQLKYLEKAFSKNGIPAILIDQALPEIEEHANLI
                     LGKLTDERMSVRFSTQKEYKDKNREDKIETLAIFISDETGSREYELFSGGEAFRINFA
                     IRLALSHILTKRAGARLQMLVIDEGFGSQDEEGRQRLIEAIIAVQDDYKKILVITHLD
                     ELKDAFPSRIEVEKTPDGSRVNVIE"
BASE COUNT         2534 a         1915 c         1890 g         2533 t
ORIGIN      
        1 aaacttcctg atttcaaagg aatcatcacc gatttaagcg gaccgacagc taatatgtat
       61 ggttttgaat gtccgaagaa actggctcaa ggtgcctgta aagacaaacg ctgcctttat
      121 ccccaacctt gcctgatgct gaaaattgat catcagcctt ctctttctct tctgaacaaa
      181 gccagaaaaa ttccgggaat caaaaaggtt tttgtcgcat ccggaattcg atacgatatg
      241 attctgcatg atccgggatg cggaaagagt tatacccgag aactttgcaa ttaccatgta
      301 tcggggcagc tgaaagtagc gcccgaacac gacagcccga aagtattaca actgatggga
      361 aagccgggaa aagagttgtt gctgcaattt aaacgggatt ttgatcagat taaccgagac
      421 accggcaaaa aacagtatct cagttattac tttattgccg cacatccggg ttgtacgatt
      481 aaagatatgc aagggttgaa aacttttgcg aatgaagaat tgcatatcgc tcccgaacaa
      541 actcaaattt tcacaccgac cccatccacc tggtcaacat taatgtatgc taccgagacg
      601 aatccattta cgggtgaaca tatttttgtt gaaaaagatc ctaatcaaaa gcagagacaa
      661 aaagatatcc ttacgcaaaa ggaacaaaaa aagacatgga caaaacactc gtaatttata
      721 accgatatgc ttgcaaagga aaatttaaat actctattca agatgttgaa aaaggattaa
      781 aagcagagga aattattgcg gatatggttt taaccgaata tccggaacat gctattgact
      841 tagttgaaaa aagtgtcaaa gaaggttatc gtcaagtgat catcgtcggt ggagacggga
      901 caatcaacga agctgtaaac ggattaattc aagcgcaaaa gaaaggctat gggaaagcga
      961 ctctgggcgt cattcaaaac ggcagaggaa atgattttgc attcggaatg aaaattccaa
     1021 acgaattgaa ggaagcaata tccctgataa aaaaagcgca accgattccg atggatgtcg
     1081 gttatgtaaa aactaatgaa aaaagtcgat atttttgtaa cggtgcagga atcggttttg
     1141 attccgcgat aaattaccgg gctaccatgt ctcatctcac aggatttcct tcctatttct
     1201 tcggattaat cggttctctg ataaaagatt tcaaacaata taacgctaaa atcagttggg
     1261 cggacggcga aacggagatg atgatcttgt tactgacgac catgaacggt acccgtgaag
     1321 gaggcggctt cctgttagct ccggattatg attttcaaga cggaaaattg aatgccgtct
     1381 taatcggaaa cgggaaacct ctacatcagt tgctgccact tattcccaga ttttttaccg
     1441 ggaatttgga tcatcccgat attctcagaa ttaagacacc ctggttaaaa gcggaattct
     1501 cgacgacagg tttatacggg caggcggacg gtgaaattat ctgttctgcg gggacggagt
     1561 tttatgcgga aatctgtccc gaaaaaattg atatcatcag cggtcttcca tttcggaaat
     1621 aggcagtctg ttataaaatt tcatcaatca tcaaaggagt gaaaaaatgg aaaaattaaa
     1681 attcggaata ttggggagcg cagggattgc ttggaaacaa tttattcccg ccattgcttt
     1741 agcgaaaaat gccgaaataa tagcggttgc cagtcgcagc gaagaaaaat caaaagattt
     1801 tgcggaaaaa tatcatattc cgctttcatt tggatcttat gaagatttac tacactcaaa
     1861 agagattcaa gcggtatata ttcctcttcc caaccgtctg cataaagaat gggcaatgaa
     1921 ggcgttcaat gccggaaagc atgtactttg tgaaaaaccc ttggcgatca ctcccgaaga
     1981 ttgtctggaa atgggcagag aagctgaaaa aaaccatgta ttgctcatgg aagcatttat
     2041 gtatcgcttt catccgttga atgaacagat tattcggaat gttcaatccg gaattttagg
     2101 cggtttggaa acgatccaag cctgccattc tttccttttg aaagacccga ataatattcg
     2161 cttgaacgct gaattagccg gtggttctct gatggatgtc ggctgctact gtgtcaatgt
     2221 tacccgaact ctgtttaatg aagaaccgga agcggttgcg gcagtagccc attatcaccc
     2281 caacggagtg gacgatcaga tgaatatttt agccaaattc aaaagcggac gctcagctca
     2341 actgagctgc ggtttgactt cggattatcc ctccggttac acaatcagcg gacggaaagg
     2401 ctatattcat gcccattatg gattccacct tggtgtttcg aacggaacgg tgacccaaaa
     2461 aatcgacgga attgaatcaa tcttaaaaga tccgtcgaat gaatatacac gaatgatcga
     2521 gcatttctcc gactgtgttt taaatcaaaa gccattacga tatacggcgg cggaagcagc
     2581 cggtaatatg gcttttatac aagccgcatt gaaatctgcc gaaaataacg gagaatggac
     2641 cgaagtctga gagatatttt aaaaatcagg tgtctgatga ttaatcgaat aaggaaaaga
     2701 atatcggatc atcctgtccg atcatttatc gttgcatccg gaatgatagc cttcctcgca
     2761 tatggattgc tgattcctca actggggatg tatggggatg atccgaattt cctgtgggcg
     2821 tatcaccggg gtggtccaaa cgaatataaa gaatttatgg gatggattcg tgaatatggg
     2881 tatctcatct attcctttct gtcacctgtt tttggagaat ccatcatttc ttggcgaatc
     2941 gccactctgg ttcttcgttg gggttcttca attctttttt tcctggtttt taacaatact
     3001 ttcccgaact ttcagaaaat agtcgttccg ctcagtatct ctttcctgct ttatccggga
     3061 tttatggaac aggcgatacc tgttgagttc ctgttacatt ttttctcatt ggatttgatt
     3121 cttgtttcac ttttatttac ttattatgga ataaagcacg gaaaaggatg gagaatcttt
     3181 ttattcttat ctgttttatc ggaaatttcc ggtcttttta tttgcgaata ttttgccgga
     3241 ttggaattat ttcggccgat aattatttgg cagacgattc atcaaattcc tcaaatatcg
     3301 gcacaatccg taaaaaataa aatcaaaaaa accggactat tcgctttccc ttatctcctc
     3361 atctttggat tttttctgta ctggcgcgca tttctttctt ccagtaccta catgagccaa
     3421 attgtaacag ttcgtctgca ggataatccg gcaatgttca tagggaatct catgcgacaa
     3481 attttatcag attttaaagt cgctgttgga gatgcctggc ttcagatttt ccaaattccc
     3541 tctccgggaa agattcgatg ggtctatctc gcgatttttt ctctcgtcgg catagtaacc
     3601 tggttgctga tcgtttctac cggtaagaag gaagaaaaac cttccgatca aaaattgtgg
     3661 gagtttttgc tgatcggaat atttcttttt ctgacaggag ggattcccgt ttgggctgcg
     3721 ggattaaaaa ttagtttgac tttatacgga gatcgcttat ctctatcatt tatggcaggc
     3781 gcatgtatca ttgtgatggc gctgttggct ttgattctcc gtcaaaaata cttccctgtt
     3841 ctgttggcga tactgatcgc attcatggcg ggttatcaat tccagcttca aaatcaattc
     3901 agaaaagaat gggatctcgc taaaaattat ttttggcagc ttgcgtggcg ggctccggat
     3961 atcagtccga atacgcttgt tttaggcgat caattcccgt tccggtctct tacggataac
     4021 tcattgaatg ctttactcaa ttggaactat gaatcgccgg aatatccgga tagggagcta
     4081 tataaatttt tccaaatctc tgcccgggaa ggaatgatcg atccgcttcc ggcaaattca
     4141 cctctttcac ataatgactt tcgcggaaat atttccgata ctatcctact ttatgcgact
     4201 cccaccactt gtttgaaagt attgacaacc gcggatcgaa atttgccgtt tctgaacatt
     4261 gcaatgaaga aaaatctttg gcgatccaag cctgaaagga ttattcccaa tccgacccga
     4321 acagtcactt ttcaatcttt tatggggact gaaccctttc acggatggtg ttactatttt
     4381 gaaaaaggtg aattggccgc tcaacagaat caatggaagg aagttgttga aatcggaaaa
     4441 gaagctgaaa gcgccggatt gattccatcc acaatgattg aaatgaaatc atttttcttt
     4501 gcggctttat cggaaaatga tttggaaaca gcggaacatt ggaaaaatct gattctgact
     4561 gagaaaggaa atgcaaatta ttttctatat cagatcaaag aatttcaaaa tcaaagccac
     4621 ctttcgaaag aggctgaatt gattttggat caaattcaag aagcttttca aagggaattg
     4681 gaatctctgt aatacaaatc aacatttttc tcagcggcgt gagaggcata agcagtttgg
     4741 atcaatctcc ttaaatttat cggggatttt tcttgagatt gattaacaaa catgccggct
     4801 tatccttaag cccgtcaatg ctgagacctt attcttgttg ttgagtcatg tccaggcatt
     4861 ctttagtata cttttaacaa tcgatcgaat aaaaaagagg aacttatgga aaagataaaa
     4921 ttcggaattt tagggacagc cggaattgcc aaattacgat ttatcccggc tattcgaaat
     4981 gcaaaaaacg ctgaagtgtc agctgtcgcc agtcgaaatt cagaaaagtc gagacgatat
     5041 gccgaagaaa acggaatcga aaccgcctat gggagttatg aagaactttt ggctcagaaa
     5101 gatattgatg ccgtatatat tccccaaccg aacgggttgc ataaggaatg gtcattaaaa
     5161 gcaattgcgg cgggaaagca cgttctttgt gaaaaaccct tagctcctac tccggaagat
     5221 tgtttggaaa tgaagcaggc agctgaagca aaaaacgtcg ttctcatgga agcttttatg
     5281 tatcggtttc atcccttaac ggaatttgtg atttcacagg ttcattcagg cgctttggga
     5341 gaaatcgaat ccattcaagc aagtttcaca tttattcttg ccgatatgga agacgtaaga
     5401 tgggatcaaa aacttgccgg cggggcggtt atggacgtag gctgctacgg aatcaatgcc
     5461 agccggacat tcttcgccga agaacccact gccgtttctg cgattgccca ttatcgaaag
     5521 cccggtatcg atgaccggat gggaattttt gcccgctttc cttcgggaaa gcaagctcag
     5581 atcatttgcg ggttcacttc ggatgctccg atcggataca ctattaatgg aaccaaaggc
     5641 tggattaccg cttataacgg tttccagaca ggggtattaa atggtacgat aacgcaggaa
     5701 atctcgggtg tccgttccac gtttcgggac agtaccaacg aatatacccg aatgatcgaa
     5761 catttttcgg attgtgtcat caaccataaa ccggtccgtt atgatgctac ggatgcagcc
     5821 cggaatatgg catttatcaa tgccgctcag aaatcagccg aaaacgacgg agaatgggta
     5881 aaagttaaag agattaaaag ttaatagcta atcacttaca cattgatgat agaaaacttc
     5941 gatttcggtt cgcgcaatct ccgaatcagc ccgttccaaa atgatttgtc gatttatttc
     6001 agcggcatcg gatcgaagtt tttcatttaa caaagcactg atcacggcgg aagccaactg
     6061 ttccggatta ttcggatcta ccaacaatcc gtttactccc ggtgtaatcc attcacgaat
     6121 ggattcgata tccccggcca caggaaaaca tccgcaagtc atcgcttcaa gcaaagaatt
     6181 gggcgtcccg tcatgctcgc tgatgctaac cgtcacttgc gcccgagaaa aaagtgacca
     6241 caaaagctct tgcggaatcg taggcagaag catcgtatta cgatgaatct ggagttcatc
     6301 caaccaagca tccgcttcag gctgcccggc cattgaacag cacagaaaaa gcgtattggg
     6361 aaattggcgt aatatcaagg ggatactttg aaaaaaagtg tcatttcgga cgcttcccgg
     6421 gcgaaaacca cgcgggttaa tcacaatatt ccagttttcc ggcaaccaat tcagtcccac
     6481 ttgttccttt tcagccgtta ttcgcacttt ggtaaaatca attccgccgt taccagggac
     6541 gactaaaatc ggctcatcgg ctggaaagcc ccaattttcc cccagtcgga tatcacggtt
     6601 tgtatccgcc gctagtcctc tgactttttt tagcgttttt tgggtcaatt gacgcatcat
     6661 tgcggaaccc tgagcatgta gtgtgatatc gtttccccaa atcgaaaccg cacacggaat
     6721 acttccccga taaaaagaaa gcagcatgcc ttcataaggg attcgtaacg catgaatcaa
     6781 atcgggattt gtttggctta cgatcctgtt gaatttccgg gcggtaagcg gtaacgaaaa
     6841 aggtcccaag atatacctga aattgatcag caacggacgg aatcttctga taaaagtccg
     6901 gtcggaccct tcggatttac tcccatcttt tgctttgttt ttgggaatcg atgaaccgga
     6961 taagcccgag aaagcgacgg gaagaataaa aaattcatcc gcctcttgcg gctgttcgca
     7021 aggataactt gaaaccagaa caatctgatg atttaagctt ttcagacttc gcaaccaatt
     7081 cagcgtaatc gggcttcttc cgtccgcaag gatcaaaatc ttcataattt ccttttttct
     7141 gtgactgaaa taaaatctac ttcgaaaata ttttattctt tccgattttg aacagcgtac
     7201 gagattgcct gttcaaatga ttccgccata ttttctaaat taatttcatt ctgcacgatc
     7261 tgaaaagatc ggtcacccat ttttcgcaac cgcgacggat cgctcagcaa agtccggatg
     7321 gcatctgtca acatttttaa atcaccgggc ttgaccaaga tgccgttttc ttcccgtacc
     7381 aaatccgtct gtgtaccgtc cgcttcggcg gcgattaccg gcaagcctga cgccattgcc
     7441 tgttgaagtg ccaatccccc tgtccctggt aagacaaaca aatcagcctg atcaaacttt
     7501 tcggtcaatt cctgcccata aagagctccg aaaaacaatg tttgcggaaa aatcttcgca
     7561 gctaattccg taatctgttg tctggccggt ccgtcgccga caatccaaag gatcgggctc
     7621 acggaatctt tcaaagcggc acaagcctcg atgagcaaat caacccgctt cctttcctgn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ttaaaggata gcgattattc
     7801 gcctctacga tcaagacatc cggattttgg cttctcaacc attgaaagat attcgtttga
     7861 aagcaaaggt aaaatttgcc atgaaatagg tgcttgttat ttgcatggta aaaatgggct
     7921 ttttccaatt tccgcgaagt atcgatcatt tcatctttcc gcggatcacc ggcaaatacc
     7981 gacacattcc cgttgtatga ttccgccaaa agatcaaaca atggaacgcg atactctgcc
     8041 agaactcgtt ggacaatgcc gattttcatt cgatcacatt tacccgcgat ccgtcaggtg
     8101 ttttttcaac ttcgattcga gacggaaagg catctttgag ttcatccaaa tgggtaatta
     8161 ccagaatttt tttataatca tcctgaacgg cgattatcgc ttcgatcaat ctctggcgcc
     8221 cttcctcatc ctgactgccg aagccttcat caattaccag catctgtaac cgggcaccgg
     8281 ctcttttggt aagaatatgg gaaagcgcca aacgaatcgc aaaattgatc cgaaacgcct
     8341 cccctccgga aaataattcg tattctcgcg atcctgtctc atcgctgata aaaatagcca
     8401 aggtctctat tttatcttcc cggttcttat ctttgtactc tttttgagtg gaaaagcgga
     8461 cagacattct ttcatccgtc aattttccca gaatcagatt ggcatgctct tcgatttcag
     8521 gaagtgcctg atcaattaag attgccggga ttccgttttt tgaaaaagct ttttcaaggt
     8581 atttcagctg agaaatttga aggttggctt cctcccgttc gcgaatgacc tcctgtttct
     8641 gctttttcaa tgactctaag acggaaacga gctgttccgc agctcctttt tccaatcgga
     8701 tgcggtttgt ttcagcctga acggtatcaa attccttctt cgccttttgt aaatccggta
     8761 agtttgccga aaattcgtcc agcgccgctt tttgctgttc cacggagcct tcaatctgct
     8821 ttaattcttt ttcatcggca gcgatctgat tggttaaatc gataatttca cgttgaatcg
     8881 gctgtaaagc ggcttttgcc tgctgtactt cctgccagcg gttttcgttc agagccaatt
     8941 cggattcttt ttcccgacat tgctcatggg cc
//
LOCUS       DAOMIV010000016         8810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8018_length_8810_cov_4.659166, whole genome shotgun sequence.
ACCESSION   DAOMIV010000016 DAOMIV010000000
VERSION     DAOMIV010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8018_length_8810_cov_4.659166"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKV59_00840"
     CDS             <1..781
                     /locus_tag="PKV59_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45325.1"
                     /translation="EYLHIDFYVQINNSKKQGAGALESKGSLCDEFSLLPDGEDYPVY
                     GGYFIDPSGRRVCSMNYFFTVENTDQFYSTVVELPRWVELDEQRFNVGCKYLYPGCKV
                     SGFRHYAWAILDEDDTYIGPKYYFCDQDRAVGNNCYEENSLWWLRVPPNTKIAVRGMV
                     NNLCNVPAANPLQNSELAVGFDVSDAGSNKWWLDGIVEGTESKDLANNSKDINFIDEP
                     SRLEDRDKDEPVDRYLLEDPSLVDLEEEAAQPIIIEETSEP"
     gene            970..1737
                     /locus_tag="PKV59_00845"
     CDS             970..1737
                     /locus_tag="PKV59_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQP45326.1"
                     /translation="MAIKVLAQDAEQTYRVKKGDTLSEIAARFNLSQEQLIKANPQID
                     PDLLMVGEELQIPPADSISYNRYLQNRYNEYLAIQPVDCYPNPDQTAECLTQIKNIGD
                     RTAYNIRLIAQTTDSEGGIRESVSSPIVNQVLPGESIPVLFRFDNRLSDTDTVQCTID
                     SLELSKDTSLSLRLDESQWSKEIVFSADKKSAKVILHFKQAAMEKTKSILIAAYNEDG
                     KVVGIRGKTNSVEQDLSLFVYSLSGMIQDISVWVEAY"
     gene            1747..2745
                     /locus_tag="PKV59_00850"
     CDS             1747..2745
                     /locus_tag="PKV59_00850"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQP45327.1"
                     /translation="MTTILVTGATGFIGSALCRELCERGYQVIGFHRFSSSLKMIEGL
                     PIQRVIGDLTDESSIENAFSYHPEIVFHLGAQQFSGRNLTRLMDVNVGGTRRIFQASF
                     NAHVKRIILMSSAVTMGTAELSPDRHRSPMPVGETHGWDRTLESWPFAWSKLLAEYET
                     QWAIAYGMDIVICNPMFVIGPGDYYRRNTGLIARFHDKPPKYFIEGGINLIHVNEVVQ
                     GLIQAMNYGEKGRRYLLSGTDRSFQQFFGDLAKLSGTPAPRMQLPTSFSNRLYAYRNR
                     HPDLFPVEKTDINLLRMAGKYFYYDGKNSRLALHLPPPQPIEPALQETLNWFRENK"
     gene            2742..3782
                     /locus_tag="PKV59_00855"
     CDS             2742..3782
                     /locus_tag="PKV59_00855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP45328.1"
                     /translation="MNRETVCLLPEIKSVGGPAAFQQRFIKAAAKQGITAHFDSSRKD
                     IGAYLVIGAPRKFIPTLYFARQRGIPVIQRLNGMNWIHRVRPSGCLYGKKADLANNRL
                     SFFRKHIATGIVYQSPFCETHWNEVYGVLSDKPSRIIYNGVDLNEFKPIVPEFSSEDI
                     EILIVEGNLEHGSEQNLEQALSLSMALSHRTSQKVHLRIAGNVPEAIREKVFERMSDS
                     TAVVSVDFLGIIEKSRLIELENSSHMLFSVELNPACPNSVIEALACGLPVIGFDTGAL
                     KDVTGVGGIIVPYGTDVWKLEKPILFPLIDAAEKVYRENQKFRKAAREQAESHFSIDK
                     ITEEYLSFLFGS"
     gene            complement(3795..4310)
                     /locus_tag="PKV59_00860"
     CDS             complement(3795..4310)
                     /locus_tag="PKV59_00860"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HQP45329.1"
                     /translation="MGPFSPSSFYTEESILAYLKVHGISYTVFHHPAVYTSAEADVYT
                     AKLPGFRAKNLFLSNNDETRHYLYICHENQRANLKQLAKIMNDSRLRFGNERNLVDFL
                     GITAGAVSLLALVNDPINRIRVIIEDEVLQAESVQLHPLVNTATVIISREGLKEFLSV
                     TGHSIFVVSSK"
     gene            complement(4317..4826)
                     /locus_tag="PKV59_00865"
     CDS             complement(4317..4826)
                     /locus_tag="PKV59_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45330.1"
                     /translation="MRKELISWLEVDKLIDHLIPQFHDEFDVILMIQKGGIIPGGILS
                     EALGIQDIYLTDIDSPKEFELEKQRSDPRFISWPTIRLFPDNSLIEQKKVLIVGCAWG
                     TGRCISAVRNRVSGAGGTPFTCVLHYNHRRNLFPEEKPDFYAAITDAWIIYPWESPKG
                     KNLILSTSV"
     gene            5105..5638
                     /locus_tag="PKV59_00870"
     CDS             5105..5638
                     /locus_tag="PKV59_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45331.1"
                     /translation="MKATRFIWFIIMIAAGIAIGLYYTWIVHPVKLVDATFYDLRQDY
                     KADYVLMTAEIYNDNPNLFQTMIRLDRLLEESPEIAVANAIRNADQLGYSETDIDMLY
                     RLNEAIGGNAIETPDAEDIFIEFDPQTFVNTETAVPTRELSAPAEMPTEDNPFIDQSL
                     SQPTLVPSDEPNPFGEP"
     gene            5638..6141
                     /locus_tag="PKV59_00875"
     CDS             5638..6141
                     /locus_tag="PKV59_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45332.1"
                     /translation="MVETGRKGPYYLLTGLIIGLILGIFYGWVINPTRYVDISPEALH
                     PDQKKQYLLLVAESYQANEDIGRSYYRIKQMTNPVNIDELRAMLLEMESDPDYAEQFE
                     VVRNFINDLDSYLKQQNPDNKISTQQVQGLLNPSDAREKGNPAMAIDEQFNKETANPE
                     SYYVNSN"
     gene            6199..6756
                     /gene="frr"
                     /locus_tag="PKV59_00880"
     CDS             6199..6756
                     /gene="frr"
                     /locus_tag="PKV59_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQP45333.1"
                     /translation="MINDIITDSENRMKSAINVLQEDLEGIRTGRATPALVEKLTVDY
                     YGMETPFNQVANISIPDAKTILIRPFETNLIRPMEKAIQASELGLTPSDDGKSIRLML
                     PPLTEDRRRDLMKLVNNRLEETRVAIRNIRRDTIKELKDLEKAKEISEDELKKAEEKI
                     QKLTDQWITQVDEIGKRKEAEIMEV"
     gene            6762..7487
                     /locus_tag="PKV59_00885"
     CDS             6762..7487
                     /locus_tag="PKV59_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HQP45334.1"
                     /translation="MAQTKLNIEPSRIPRHVAIIMDGNGRWAKKRGLPRLAGHRQGVE
                     NLRQIIKACVEFGIQYLTVYAFSTENWGRPEDELRGLKQIFNDAFSNELEELIKNGVR
                     ILHIGQRDEISPDLLQKIDRAVEISKTNDKLILTVALNYGSRNEIMHAVRKIVASGIP
                     PEEVTEQVISDSLFTAGTPDPDLVIRTSGEQRLSNFLLWQSAYSEWVFPEAYWPDYNR
                     EELIKSLEEYAKRDRRFGKLSSK"
     gene            7491..8318
                     /locus_tag="PKV59_00890"
     CDS             7491..8318
                     /locus_tag="PKV59_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HQP45335.1"
                     /translation="MDNLKTRVISAAVLAPLFIFLIYLGGIPFLFASLLIIGIGSWEF
                     WRMYNKEQSNRSSAVLLIGLSILTGASRYFLDFTIASSVLALCIMIAMLWGNISYERK
                     NQKAADGFAVMTTGIVYFGWIGSYLITLRQSENGFWWVIICLLLVWLSDTGAFFVGSK
                     FGKHQMAPLVSPHKTWEGYFGGVLSTLVIGILLFLIIPPVSKILKFPQMILLALLIGL
                     ITPFGDLGESMIKRTFGFKDSSNLIPGHGGIFDRMDTVFWTMVIGYSMYTLINLGLI"
     gene            complement(8299..>8810)
                     /locus_tag="PKV59_00895"
     CDS             complement(8299..>8810)
                     /locus_tag="PKV59_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45336.1"
                     /translation="MTGTIRLAEGESFNDKIDQSDSGSIYMMNGTAASQTGSSEAIWD
                     LQLNRQIPVVLMVNMANGYQMVDLRDLDVAGVNLISQVGNIDVMLPYAASVPVNISTQ
                     SGDIRIFVPASVSAYVTVQNATDVFYPETYMQSGSQIFPANGLMNGSQVVVNANAPAG
                     VVKVILNPN"
BASE COUNT         2591 a         1831 c         1982 g         2406 t
ORIGIN      
        1 ggaatatttg cacatcgatt tttatgttca gattaacaac agtaaaaaac aaggtgcggg
       61 ggctttggaa tccaaaggaa gcctttgtga cgaatttagt cttctgcctg atggggaaga
      121 ttatcctgta tacggcggct attttatcga tccgtcggga agacgggttt gctccatgaa
      181 ttatttcttt actgttgaaa atactgatca attttactct acggttgttg aacttccgcg
      241 ctgggtagaa ctggatgaac aaagattcaa tgtcggatgt aaatatcttt atccggggtg
      301 taaagtttca ggcttccgcc attatgcatg ggcgatactg gatgaggatg atacatatat
      361 cggcccgaaa tattattttt gcgatcaaga ccgggcggta gggaataatt gttatgaaga
      421 aaattcctta tggtggctgc gggttcctcc caatacaaag atagccgtcc gggggatggt
      481 caataattta tgcaatgtcc ctgctgcaaa cccgctgcaa aactcagaac ttgcagtcgg
      541 tttcgatgtc agtgatgccg gttccaataa atggtggctg gatggcattg ttgaaggaac
      601 cgagtcaaaa gacctggcaa acaactcgaa agatatcaat tttatagatg aaccttcaag
      661 attagaagat cgggataaag atgagcccgt tgaccgctat ttattggaag atccatccct
      721 ggttgatttg gaagaggaag cggcacagcc gataattatc gaagagacat cggaaccgta
      781 aaattatagc gtcgataact tttaaaaagg aggccggatc atttttcggt agcctccttt
      841 ttatttaatg cattcgaggg aaactctttc ctaattttgt tgatttcggt taacagtctg
      901 tggtacgatc aaatcatgaa atattttcgt ttttcggctt tcttttttat tcttttggta
      961 aatttcttta tggctattaa agttctcgct caagatgcgg agcaaactta tcgggtaaaa
     1021 aaaggagaca ctctttcgga aattgccgcc cgatttaatc tgagtcaaga gcagttgatc
     1081 aaggcgaatc cccaaatcga tcccgattta ttaatggtgg gagaagaatt acaaattcct
     1141 cccgcagatt caatttctta taatcggtat cttcaaaatc gatataacga ataccttgcg
     1201 attcagcctg tggattgtta tccgaatcct gaccaaaccg ctgaatgttt gacacaaatc
     1261 aaaaatatcg gcgaccgtac cgcttacaat atccgattga tcgcgcagac cacagattcg
     1321 gaaggaggaa ttcgggaaag tgtcagtagt ccgattgtca accaagtact tccgggtgaa
     1381 tcgatacccg ttttattccg tttcgataat cgattatcgg atacggacac tgttcaatgt
     1441 acaattgatt cgttggaatt atcgaaagat acgtctctat ctttgcgctt ggatgagtct
     1501 caatggtcaa aggaaatagt tttttcagcg gacaaaaaaa gcgcaaaagt aattcttcat
     1561 tttaaacaag ccgctatgga aaaaacgaaa tcaatcttaa tcgcagctta taacgaagac
     1621 ggaaaagttg tcggcatccg cggaaaaaca aattcggttg aacaggatct gtctcttttc
     1681 gtttattccc tgagcggaat gattcaggat atatccgttt gggttgaagc ctattaaaga
     1741 ttaaaaatga caacgatctt agttaccggt gcaaccggct ttatcggatc agctctttgt
     1801 cgggaactat gcgagagagg atatcaggtg atcgggttcc atcgttttag cagttcattg
     1861 aaaatgatcg aaggactgcc catccaacgg gtcatcggag atttaaccga tgaaagttcc
     1921 atagaaaatg ctttttccta tcatcccgag attgtctttc atttgggcgc gcagcaattt
     1981 tccggcagga atctgacccg tctgatggat gtcaacgtcg gaggaacacg aaggattttt
     2041 caagcttctt tcaatgctca cgtcaaacga attatcctga tgagctctgc cgttacgatg
     2101 ggaacggctg agctttcacc ggacaggcac cgttcaccga tgccggtcgg tgaaacacac
     2161 ggatgggatc ggactctcga aagttggccg ttcgcttggt caaagttatt ggcggaatat
     2221 gaaacgcagt gggcaatcgc ttatgggatg gatatcgtca tctgcaaccc catgttcgtg
     2281 atcggaccgg gggattacta tcgcagaaat accggtttga ttgcccgatt tcatgataaa
     2341 ccgcccaaat attttatcga aggcggcatc aatttgatcc atgttaatga agttgtacaa
     2401 ggtttgattc aggcgatgaa ttacggtgaa aaaggccggc gttacctttt atccggaaca
     2461 gaccggtcgt ttcaacaatt ctttggcgat ctggcaaaat tatcggggac tcctgcacct
     2521 cggatgcaac tcccgaccag tttttcaaat cgtttgtatg catatcggaa ccgacatccg
     2581 gacttattcc ccgtcgaaaa aacggatatc aatctgcttc gaatggcggg aaagtacttt
     2641 tattatgacg gcaagaattc cagattggcg cttcatcttc ccccgccgca gccgatagaa
     2701 ccggcattac aggagacttt gaattggttt cgggaaaaca aatgaatcgg gaaaccgtgt
     2761 gtcttttacc ggaaataaaa tccgtagggg gaccggctgc ttttcagcaa cggtttatta
     2821 aagccgccgc caaacagggt atcactgccc atttcgattc ttcgcggaag gatattggtg
     2881 cttatttggt aatcggagct ccgcgaaaat ttattccgac cctttacttt gcacgacaac
     2941 gggggattcc ggttattcag agactcaacg ggatgaactg gattcatcgt gtccgaccgt
     3001 ccggctgctt gtacgggaaa aaagcggatc tggcaaacaa cagactttct tttttcagga
     3061 agcatatcgc caccggaata gtctatcaaa gccctttttg tgaaacacat tggaatgaag
     3121 tttacggagt tttatcggat aaaccttccc gaattatcta taacggtgtt gatttaaacg
     3181 aattcaaacc gattgtcccc gaattttcgt ccgaagatat cgaaatctta attgtcgaag
     3241 ggaaccttga acatggttct gagcaaaatc tggaacaagc attatcgtta tcgatggcat
     3301 tgtcccatcg aacttcacaa aaagtgcatt taagaatcgc cggaaacgtt cccgaagcta
     3361 ttcgagaaaa agtttttgag agaatgtcgg attcaaccgc cgtagtatcc gtcgactttt
     3421 tgggaatcat cgaaaaatca agattaatcg aactggaaaa ttcatcccat atgttgtttt
     3481 cggtagaact aaaccccgcc tgtccgaatt cagttatcga agctctggct tgcggattgc
     3541 ccgtcatcgg atttgatacg ggggcgttga aagatgttac cggtgtcgga gggattattg
     3601 tcccctatgg aacggatgtt tggaaattgg aaaagccgat ccttttccca ttgatcgacg
     3661 cagcggaaaa agtctaccgg gaaaaccaaa aatttcgtaa agcggctcgc gaacaagccg
     3721 aatcacattt ttcaatcgat aaaattaccg aagaatattt atcgttttta ttcggatcat
     3781 aagaaaaatc aatctcattt cgacgaaacg acaaaaatag aatgaccggt aacagataaa
     3841 aactctttta acccttcacg gctaattatg acggttgctg tgttcaccaa aggatgtaat
     3901 tggactgact cggcttgtaa aacttcatct tcaatgatta cacggatgcg attgattgga
     3961 tcattgacta atgccaaaag tgaaactgct ccggcggtaa taccgagaaa gtcaaccaaa
     4021 tttctttcat ttccgaaacg aagccgcgaa tcattcatga tttttgcgag ttgtttcaga
     4081 ttcgctcgct gattttcatg gcagatataa agataatgcc gtgtctcatc gttattggaa
     4141 agaaaaagat ttttcgctcg aaaacccggc aattttgcgg tataaacatc cgcttccgca
     4201 ctggtataaa cagcgggatg atgaaaaaca gtataagaaa taccgtgaac cttcaaataa
     4261 gccagaatag attcttcggt ataaaatgat gaaggcgaaa acggacccat ccgaaactat
     4321 acggaagttg atagaatcag atttttcccc ttcgggcttt cccagggata aatgatccaa
     4381 gcatcggtaa tcgcggcata aaaatccggt ttttcttcgg gaaacaaatt ccttcgatgg
     4441 ttataatgca atacacacgt gaaaggcgtc cccccggcac cgctgacgcg attgcgcacc
     4501 gcagaaatac aacgaccggt tccccaagca cagccgacga ttaacacttt tttctgttcg
     4561 atcaacgaat tatcggggaa aaggcggata gtcggccacg aaataaatcg cgggtcactg
     4621 cgctgttttt ccaactcaaa ttccttggga gaatcaatat ccgtcagata aatatcttgg
     4681 atccctaatg cttccgacaa aatcccgcca gggatgattc cccctttttg gatcatgaga
     4741 attacatcga attcatcatg aaattggggg attaaatgat cgatcaactt atcgacttca
     4801 agccaggata ttaattcttt tcgcatattt tattcaatcc agtgtgaatt atagttttga
     4861 aatgtgaatt aagctatgaa gatttttatt ctataaacaa ggcatccatt cattgaaatt
     4921 taatctttcc gttctgtcaa tattaaaagg cattcagccg tcatatcgat tcgccgaaaa
     4981 ttcgaagaaa atgatcagtc catggtttcg gtattttgtt gtcttcaaat gagttgtatt
     5041 cggcataagt ctaaactata ataaggaagg taaaaatatt aatgctggaa agattcattc
     5101 gaccatgaaa gccacaaggt tcatttggtt tatcatcatg attgcagccg gaatcgcaat
     5161 cgggctgtat tatacttgga tcgtccatcc cgtcaaactg gtagatgcaa cattttatga
     5221 tctgaggcag gattacaaag ctgattatgt gctgatgact gccgagatat ataacgataa
     5281 ccccaatctc tttcagacta tgattcgact tgatcgattg ttggaagaat ctccggaaat
     5341 tgccgtcgcg aatgccattc gaaatgccga tcaactgggg tactctgaaa cagatatcga
     5401 catgctttat cgcctgaatg aggcaatcgg cggaaatgcg attgaaaccc ccgatgccga
     5461 agatattttt atcgaatttg atccgcagac atttgtaaat acggagaccg ctgtgccgac
     5521 ccgggaatta tcagcgccgg cagaaatgcc gacggaagac aaccccttta tagatcaatc
     5581 cctgtcacag ccgacgctcg tcccatccga tgagccgaat ccgttcggag aaccgtaatg
     5641 gtagagacag gacggaaagg accctattac ttattaaccg gtctgatcat cggattaatt
     5701 ctcgggatat tttacggttg ggtcatcaat ccgactcgtt atgtggatat ttctccggaa
     5761 gcgctccacc cggatcaaaa gaaacaatat cttttattgg tagccgaatc ctatcaggca
     5821 aatgaagata tcggccgatc ttattacaga atcaaacaga tgacgaaccc ggtgaatatc
     5881 gatgagctgc gggcaatgct cctcgaaatg gaatcggacc cggattatgc cgaacaattc
     5941 gaagtggtcc gtaattttat aaatgatctg gacagctatc tgaaacagca aaatccggat
     6001 aacaaaatct ccacacaaca ggttcaaggg ttgctaaatc cgagcgatgc ccgggaaaaa
     6061 gggaacccgg caatggcgat agatgaacaa tttaataaag aaaccgcaaa tccggaaagc
     6121 tattatgtaa attcaaatta acccaaaggt ataattaacc gaccgaacaa atctgagaca
     6181 tacttcacgg aggattcgat gattaatgac attattacag attccgaaaa cagaatgaaa
     6241 agtgcgatca atgttttaca ggaagattta gaagggatca ggacgggcag agcgacaccg
     6301 gcactggttg aaaagctgac ggttgactat tacgggatgg agacaccctt taatcaagtc
     6361 gctaatatca gtattccgga cgcaaaaacg atcttaattc ggccgttcga aacaaacttg
     6421 atccgtccaa tggaaaaagc gattcaagca tccgaactcg gtctgactcc gagtgatgac
     6481 ggtaaatcaa ttcgtttgat gttacctcct ctgacggaag atcgcagaag agatttgatg
     6541 aaattggtga ataaccgtct tgaagagaca cgggtagcga ttcggaatat ccgccgcgat
     6601 accataaaag aactcaaaga tttggaaaaa gcaaaagaaa tttcggaaga cgaactgaaa
     6661 aaagccgaag aaaaaatcca aaaattgacg gatcaatgga ttacccaagt agatgaaatc
     6721 gggaaacgga aagaagcgga aatcatggag gtctgaccga tatggcacag acaaaattga
     6781 acatcgagcc ctcaagaatt ccgcgacatg ttgccatcat catggacgga aatgggcgct
     6841 gggcaaagaa aagaggattg ccaagacttg ccggacatcg ccaaggtgtc gaaaatctca
     6901 ggcaaatcat caaagcctgt gttgaattcg gaattcaata tctgaccgtc tatgcgtttt
     6961 caacagaaaa ttggggacgg cctgaggatg aattaagagg attaaaacag attttcaatg
     7021 atgctttctc taacgaactc gaagaattga tcaagaacgg tgtgcgaatt ttacacatcg
     7081 ggcagcgaga tgaaatcagc ccggaccttc tgcaaaaaat tgatcgagcg gtagaaatct
     7141 caaaaacaaa cgacaaatta attctgactg tcgcgttgaa ttacggcagc agaaatgaaa
     7201 taatgcacgc cgtacgaaaa attgtcgctt ccggaatacc tccggaggaa gtaacggaac
     7261 aggtcatcag cgattcactg tttaccgcgg ggacgcctga tcccgacttg gttatccgca
     7321 catccggtga acagcggctc agcaattttt tgttgtggca gtcagcttat tcggaatggg
     7381 tttttccgga agcttattgg ccggattata atcgagagga attaataaaa tcactggaag
     7441 aatatgcaaa gagagatcgc aggtttggaa aactctcttc aaaataaaac atggataatt
     7501 taaaaacccg cgtcatctca gcagctgttc ttgccccctt gttcattttt ttgatttatc
     7561 tgggaggaat tccttttctc tttgcatccc ttctaattat cggcatcggc tcctgggagt
     7621 tttggcggat gtacaataag gaacagtcaa accgttccag tgcggtttta ctgatcggct
     7681 tatccattct gacaggggca tcccgttatt ttttggattt tacaattgca tcgtctgtgc
     7741 ttgcgctttg tattatgatc gccatgcttt ggggaaacat ttcctatgaa cggaaaaatc
     7801 agaaagccgc ggatgggttt gcggtgatga ccacgggaat tgtttatttt ggctggatcg
     7861 gcagttactt gattacactt cgccaatcgg aaaatggttt ttggtgggtt attatttgcc
     7921 tgctgttagt ctggttatct gataccgggg cttttttcgt gggcagcaag ttcggaaagc
     7981 atcaaatggc acctcttgtc agtcctcaca agacttggga agggtatttc ggcggcgttt
     8041 tatcgacttt agttattgga attcttctgt ttctgatcat tccgcctgtt tcaaagatcc
     8101 tcaaattccc gcaaatgatt ctgttggcac tcctgatcgg tttgatcact cctttcgggg
     8161 acttgggaga gagcatgata aaacgtacct ttgggtttaa agattcttca aatttaatcc
     8221 cgggacacgg cgggattttt gaccgaatgg acaccgtatt ttggacgatg gtaatcggat
     8281 attcgatgta taccttaatt aatttgggtt taatataact tttacgacac ccgccggcgc
     8341 atttgcattc acaacaacct ggctgccatt catcaagccg tttgccggaa atatctgaga
     8401 tcccgattgc atatatgtct ccggatagaa tacgtcggtt gcattttgga ctgtaacata
     8461 agccgacacg gaagcgggaa caaaaatccg aatatctccg ctttgtgtac tgatattcac
     8521 cggaacactt gcggcataag gaagcatcac atcgatattt ccgacctggg atattaaatt
     8581 gacgccggct acgtctaaat cgcgcaaatc aaccatttga tagccattcg ccatatttac
     8641 cattaataca accggtattt gtcgattaag ctgtaaatcc caaatcgctt ccgaagaacc
     8701 ggtttgagaa gcggcagttc cgttcatcat atagatggaa cccgaatcac tttgatcgat
     8761 cttgtcattg aaggattcgc cttcggcaag tcgaatcgtc ccggtcataa
//
LOCUS       DAOMIV010000017         8790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8046_length_8790_cov_4.727075, whole genome shotgun sequence.
ACCESSION   DAOMIV010000017 DAOMIV010000000
VERSION     DAOMIV010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8046_length_8790_cov_4.727075"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="PKV59_00900"
     CDS             complement(<1..682)
                     /locus_tag="PKV59_00900"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQP45337.1"
                     /translation="MLKKNLGIVILAVLISFIFGTVVFAEDEWVPFEAGQENVQPMEC
                     TIVKPIGPNENITAALPPITDEDWSIGPKDAPLTILEYSDFQCPYCSNAGLALLEFQK
                     NHPDEVRYVYRHFPLSFHEKAPMAAYAADAAGAQGLFFEAEHLLYSTQSDWSGLESLD
                     AFETWLKDNFKTQLSGLNYDQWLTDFADPDLRAKVDKAFDEVAATGVVPGTPTIFLNF
                     NSYQGSYDE"
     gene            complement(820..1437)
                     /gene="tmk"
                     /locus_tag="PKV59_00905"
     CDS             complement(820..1437)
                     /gene="tmk"
                     /locus_tag="PKV59_00905"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQP45338.1"
                     /translation="MFITFEGPDGSGKTTQVKRIGRKLIEKGFDIVYTREPGGTEISD
                     QVREILMNMKNKQMCPRTEILLFCAARAQLVEEVIRPALQSGKIVISDRYADSTLAYQ
                     GYGHGFEQETLKQLLNFTTGNLWPDLTFLLDISAEKGLRRRISNQEEWNRMDDYQLAF
                     HERVRNGYHQLAAADPQRWEIIDADQSEEAVENEIMQRLARRLHF"
     gene            complement(1441..3144)
                     /locus_tag="PKV59_00910"
     CDS             complement(1441..3144)
                     /locus_tag="PKV59_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HQP45339.1"
                     /translation="MKQYENNLDDIHALVAIFPEEIGEKIESIGNLSELVEVVFDVGR
                     PPLARYTDDEIQLSEKEVAFEDLETIISQIGDFDLDNRAGMERTLHRISAVRNRKGKI
                     IGLTCRVGRAVFGTLEIIQDFVDSGKSILILGKPGIGKTTMLREAARVLAERKRVVIV
                     DTSNEIGGDGDVPHPAVGKARRLQVAKPALQHEVMIEAVENHNPEVIVIDEIGRELEA
                     QAARTIAERGVQLIGTAHGRTLSNLLMNPTLSDLVGGIESVTLSDEEARRRGTQKTVL
                     ERRSPPTFDVLVEIVERNKVVVYPDVAAAVDALVRGIELAPELRIKTDQGKIIREKAP
                     APVLEGNGFTPAAESDLQIRQKRNILQISGRSKTESGGRRNSRRGVSAESYGMTQSTS
                     PVLTGERDLDSDSQNDPGMKPIRIYPHGVARNRLEQASARLGVLISIVNNVEEADVLM
                     TLRPYYRSRQKMIVDAEERNVPIYVLRSNTINQIEYTLANLFNVTVQQPSGNWENVEK
                     ETTEAIMAVKNGEAVWVDLAPANRMIRQMQHELVEQYGLLSQSYGKEPKRRIRIFQNP
                     E"
     gene            complement(3141..4133)
                     /locus_tag="PKV59_00915"
     CDS             complement(3141..4133)
                     /locus_tag="PKV59_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45340.1"
                     /translation="MWWFSESLNGGIERNFRIVVAQARTFRLYDMKFFLETENKYYCL
                     SNRTRFTNNQFSLTTCFLDSLNLLSSESIAVSRGPYLTYYWGGQISFDMKKNTAWFSW
                     LIAGKHSNNSNLLELLEFLVTKAGKAGMQSCAVVLDEGQWVTGSFRQIGFSVYSKQTV
                     WCYKGELNSDGENWQSVSVTPFDEYDLFYESQITPLIRQLGSNWNQENIYALKKDGMI
                     QATAKATAQNGNRVYLEPIFNPGVENPAKLMLSLAQQINHTTDQEVYVMIPGFQAWAN
                     DPFNEENFNLVCRQTVFVKNLAAKVAEEKPAIQLENGYLQPVGPANYQMNKKEK"
     gene            complement(4096..4890)
                     /locus_tag="PKV59_00920"
     CDS             complement(4096..4890)
                     /locus_tag="PKV59_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQP45341.1"
                     /translation="MNRIREKLFLFPWMLCIPLGILGLLLIFEQPAFAYQALASPTPD
                     FDGNIFYTVSDLDTCLSISLKMGVDVETLRSLNNLDVNCTLIAGTKILLGRVLTPTPT
                     AGPSPTPTEILPTPTPYDGSAQLCIYLFEDKNGNATAEETETGIAGGAVSVTKRGGTE
                     SFTGLTTGLDLLCFPEVPEGDYNISVAPPKEYNPTTNMNYPITIKAGDTTQVNFGAQK
                     QAAFENSAAATQQQTKKSPLLAIIGIVLILIGVGIVVVFGILKRRD"
     gene            complement(4904..6661)
                     /gene="recN"
                     /locus_tag="PKV59_00925"
     CDS             complement(4904..6661)
                     /gene="recN"
                     /locus_tag="PKV59_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQP45342.1"
                     /translation="MPFADAEMLTELYIRNFAIIDELELSLKEGLNIFTGETGAGKSI
                     ILDAVSTVVGGKTDPTFIREGTDRAIVEAVFRPDENREAIREILKREDLPEDSDGDQI
                     LLSRDIRAGGRSSARVNGSSVNVNLLREIGRLLVDIHGQSDHLSLLNPSNHIVLLDRF
                     AGNQELLKKYQAEFKKLQKIRTRLTEIRMNDEELLRKKDMLSFQMNEIRSAKINESEE
                     ETLKKDRDRLANAENLSQLTQKAMEQLEGRSEEYPGIFELLGYLSKSMDNLSRIDPEL
                     APLGEEVAAINDALNELLASLQRYQETLEFNPARLDAIEERLALLNNLKRKYGGSLHA
                     VLEFATRAEEQLAGLENISDQTEDLIRQETSVINLLSELAQELSDRRHEASRKVSESV
                     EKELHELRMESAQFEIRFTQTAAENGLRDRHGRSLAYDENGFDQVEFLIAPNPGEGLK
                     PLAKIASGGETSRLMLALKNTLAEADSIPSMIFDEIDQGIGGRVGSIVGEKLWLLSRN
                     HQILCITHLPQLAAYYDAHFHVSKQLSDGRTRTLVKQLQPEESLREMAQLMGGDTPEN
                     RRASGAMILNAQKWKESVK"
     gene            complement(6642..7205)
                     /locus_tag="PKV59_00930"
     CDS             complement(6642..7205)
                     /locus_tag="PKV59_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45343.1"
                     /translation="MSDEISEIKTCYKHPNRETLICCNQCGRPICMDCAVSTPTGYRC
                     KECVKEQQKKFNTAVSRDYVLSAIIAFLIGAVGSFVLYFFNFYSMLTAIILGLLLGNL
                     IAKAVRAVTQKRRAADLNKLTAIAAGIGGAVPILRYILGLFRMIFIGNYAGLMGYTLS
                     VGWGILYIALLCTTILTNMRGFAIRRR"
     gene            complement(7202..8188)
                     /locus_tag="PKV59_00935"
     CDS             complement(7202..8188)
                     /locus_tag="PKV59_00935"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQP45344.1"
                     /translation="MIRSKKFIVLIAIHSAAGVADTDRFVMKNCEPKMNQTTKKPERI
                     GVTWHPHLAAAEEEAKKIIEEINTRFHGQAVAEGWSLYDEAFRESLERRPFDLVITFG
                     GDGTMLRTNKLCAPFEVPVCGINMGSFGFMMEIAKNQWREKLPALMNGDWRIEKRMML
                     HVELVRKDNERTRWEVLNDAVIGHGSKLRPIRINVRINENYLTDYVADGLIVSTATGS
                     TAYAMAAGGPIMEPELRNILVIPVAPHLCLERGIVLDENDVVEIRSSSEHETIFSPDG
                     QDGMMIDESDTISVSASEFSARYVRLEGKGFFYSNFIQYMQRNPSANRKTTI"
     gene            complement(8097..8615)
                     /locus_tag="PKV59_00940"
     CDS             complement(8097..8615)
                     /locus_tag="PKV59_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HQP45345.1"
                     /translation="MNQQLLLGILLLALALAGLILTERKKASDVLFQGKPVYEDIAPN
                     GKLKAPLFDPETRLAGKPDLLIQKGEQLIPIEIKSQPAPETPPASHVFQLMAYCLLTE
                     KAFAVKPDYGILRYRNKEFKISYTIKRRDELLDRLERIREDDSLEEVHRSHCDSFRCG
                     GCGYRSICDEKL"
BASE COUNT         2188 a         2417 c         1859 g         2326 t
ORIGIN      
        1 cttcatcata cgaaccttga tagctgttga aattcaagaa aatcgtcggt gtacctggaa
       61 ccactcccgt tgcggctact tcgtcaaaag ccttatcaac cttcgctctt aaatctggat
      121 cggcaaaatc agtcaaccat tgatcatagt ttaatcccga aagctgagtc ttgaaattat
      181 cctttaacca ggtttcgaaa gcgtctaaag attccaaccc gctccaatcg gattgagtag
      241 aataaagcaa atgttccgct tcaaaaaata atccctgagc tccagcggca tccgccgcat
      301 aagcagccat cggcgctttt tcgtgaaagc tcagcgggaa atgacggtag acataccgaa
      361 cctcatccgg atggttcttt tgaaactcta acagagccag cccagcatta gaacagtagg
      421 ggcattggaa atctgagtat tctaaaatcg tcagaggagc gtctttcggc ccaatgctcc
      481 aatcctcatc cgttatcggg ggtaacgcag ccgtaatatt ttcattcgga ccaatcggtt
      541 taacaattgt acattccatc ggctgaacgt tttcctgacc ggcttcaaag ggaacccact
      601 catcttcggc aaaaacaacg gtgccgaaga taaaactaat taacaccgca agaataacaa
      661 tacctaaatt ctttttaagc atagatcatt ttcctttcag aataggcctt acacaagatg
      721 attttatacc aaatcttttc gggatggtat ctttaacaaa gttttaagca aataattacg
      781 gctacagacc cgttttaatt actttccgaa tatttttctt caaaaatgaa ggcgacgggc
      841 aagccgttgc ataatttcat tttcgacagc ttcttcggat tgatcggcat cgattatctc
      901 ccaccgttgc ggatcggcag cagccaattg atgatagcca ttgcgaaccc gctcatggaa
      961 cgcaagctga taatcatcca tccgattcca ttcttcctga ttgcttattc tgcgtctcaa
     1021 tcccttttcg gcggatatat ccagcagaaa cgtcaaatca ggccaaagat tgcccgtggt
     1081 aaaattgaga agttgtttca atgtttcctg ttcaaatccg tgaccgtatc cctgataagc
     1141 caaggttgaa tccgcatagc ggtcactgat aacaatcttt ccgctttgaa gggcaggacg
     1201 aatcacttct tccaccagtt gagcgcgagc cgcacaaaaa agcaggatct ccgtccgcgg
     1261 gcacatctgt ttgtttttca tattcatcag aatttcccgc acctgatcac tgatttcggt
     1321 cccccccggt tcgcgggtgt agacgatatc aaatcctttt tcgataagct tccgcccaat
     1381 tcgtttcact tgcgtggttt ttccggatcc gtccggtcct tcaaaggtaa taaacatgtt
     1441 ttactccgga ttctgaaaaa tgcggattct gcgtttgggc tctttcccgt aagattgcga
     1501 taataacccg tactgttcga ccaattcatg ctgcatttgc cgaatcatgc gatttgcggg
     1561 cgccaaatca acccagactg cttccccgtt tttcacggcc ataatggctt ccgtagtttc
     1621 tttttcaacg ttttcccaat taccggacgg ctgttgaacg gtcacattaa acaagtttgc
     1681 caatgtgtat tcgatctggt tgatcgtgtt tgaacgcaga acatagatcg gcacattccg
     1741 ttcttccgcg tccacaatca ttttctgccg gctgcggtaa tagggacgta aagtcattaa
     1801 gacatccgct tcctcgacgt tattcacaat ggaaatcaag actcccagcc ttgcggaagc
     1861 ctgttccaaa cggttacggg ctacgccgtg gggataaatc cggatgggtt tcatgcccgg
     1921 atcattttga gaatccgaat ccagatcccg ttcaccggtc aaaaccggtg atgtcgattg
     1981 ggtcatccca taggattccg cggatacgcc ccgccgtgaa ttccgacggc cgccgctttc
     2041 cgttttactg cgaccggaaa tctgaagaat attccgtttt tggcggattt gcagatcgga
     2101 ctcggctgcg ggtgtaaacc cgttcccttc caacacggga gccggcgcct tttcgcgtat
     2161 tatttttccc tgatccgtct ttatccgcaa ctccggtgcc agttcgatcc cgcggaccaa
     2221 cgcatccacc gccgccgcca catcgggata aacaaccacc ttatttcgtt cgacaatttc
     2281 caccagcaca tcgaaagtcg gcggagaacg ccgttccaga accgttttct gcgttccgcg
     2341 gcgtcgcgcc tcttcatccg acaacgtcac cgattcgatt ccgccgacca ggtcggagag
     2401 ggtcgggttc atcagcagat tgctcaaggt tcggccgtga gccgtcccga tcaactgaac
     2461 cccgcgctcc gcgatcgtac gcgcagcctg cgcttccaat tcacggccga tttcatcgat
     2521 cacgatcact tccggattat ggttttcgac cgcttcgatc atcacctcat gctgcaaagc
     2581 cggtttcgcc acttggaggc ggcgcgcctt tcccaccgcc ggatgaggca catcgccgtc
     2641 cccgccgatt tcattggaag tgtcgacaat gacgacccgt ttcctttccg ccaacactct
     2701 ggccgcttcc cgtaacatgg tcgtcttccc gatcccgggt ttccccaaaa tcagaatact
     2761 tttgcccgaa tccacaaaat cctgaatgat ttcgagcgtc ccaaataccg cacggcccac
     2821 tcggcaggtc aatccgatga ttttcccttt ccggttgcga accgccgaaa tccgatgcag
     2881 cgtccgttcc attcccgccc ggttgtccaa atcgaaatcg ccgatttggg aaataatcgt
     2941 ctctaaatct tcaaaggcga cttccttttc ggaaagctga atctcatcat ccgtatagcg
     3001 agccaagggc ggacgtccca catcaaaaac cacttctacc aattcagata gatttccgat
     3061 actttcgatc ttttctccga tctcctcggg gaaaatcgcc actaacgcgt ggatatcatc
     3121 taaattattt tcgtactgct tcatttttcc tttttattca tctgataatt tgccggtccg
     3181 accggttgaa gataaccgtt ttccaattgg atcgccggtt tttcctcagc cactttcgcg
     3241 gcaagatttt ttacaaaaac tgtctggcga cataccagat taaaattttc ctcattaaac
     3301 ggatcgtttg cccaagcctg aaaacccggg atcatcacat aaacctcctg atccgtcgta
     3361 tggttgattt gttgtgcgag gcttagcatt aactttgccg gattctcgac ccccgggttg
     3421 aaaataggct ctaaatagac ccgattcccg ttttgagcgg tcgcctttgc cgtcgcttgg
     3481 atcataccat cttttttaag cgcatagata ttttcctgat tccaattcga acccaactgg
     3541 cgaatcagcg gcgtaatctg cgattcgtag aacagatcgt attcatcaaa aggcgtaacg
     3601 ctcacagatt gccaattttc tccgtccgaa tttaattctc ctttataaca ccaaaccgtc
     3661 tgtttggaat aaacgctgaa tccgatctga cggaatgaac cggttaccca ttgcccttca
     3721 tccaaaacca ccgcacaaga ctgcattccg gcttttcccg ccttggtcac cagaaattcc
     3781 aacaactcca acaggttcga gttattggaa tgtttcccgg caatcaacca cgaaaaccaa
     3841 gccgtatttt tcttcatatc gaaactgatc tgcccgcccc aataataggt cagataaggc
     3901 ccgcgcgaaa cggcgatcga ttcgcttgaa agcaaattga gagaatccaa aaaacaggtt
     3961 gtcagcgaaa actggttatt ggtaaaacgg gttctgtttg aaagacaata atatttgttc
     4021 tccgtttcaa ggaaaaactt catatcataa agtcgaaatg ttcttgcctg agccactaca
     4081 atccgaaaat tcctctcaat cccgccgttt aaggattccg aaaaccacca caatccctac
     4141 tccgatgaga atcaaaacaa tgccgatgat cgccaacaac ggcgatttct tggtctgctg
     4201 ttgggtcgcc gcagccgaat tttcaaatgc ggcttgtttt tgcgcaccga aattgacctg
     4261 agtcgtatca cccgctttga tcgtaatggg ataattcata ttggtcgtcg ggttatactc
     4321 cttcggcgga gcgacgctga tgttatagtc cccttccggc acctccggaa aacacaacag
     4381 atccaatccg gtcgtcaaac cggtgaagga ctccgtccct cctcgtttcg taacgctaac
     4441 ggctccgccg gcgattccgg tttcggtctc ctccgcagtg gcgttcccgt tcttatcttc
     4501 gaacagatag atacagagtt gtgccgaccc gtcataaggc gtcggcgtcg gtaaaatttc
     4561 ggtcggcgtc ggcgaaggac ccgctgtcgg tgtgggggtc aagactctgc ccaaaaggat
     4621 cttcgttccg gcaatcagcg tgcaattcac atccaaatta ttcagcgagc gaagcgtttc
     4681 gacatccaca cccatcttca gtgaaatact gagacaggta tccaagtcgg acaccgtata
     4741 aaaaatattg ccgtcaaaat cgggcgtcgg cgacgccaaa gcctgatagg caaaagctgg
     4801 ctgttcgaag attaacaaca accccaaaat tcccaacgga atgcacagca tccaaggaaa
     4861 caaaaataat ttttccctta ttctattcac gattcaaatt atatcatttg acgctttctt
     4921 tccatttttg ggcattcaga atcatggcgc cggatgcccg ccggttttcc ggggtatctc
     4981 cacccattaa ttgcgccatt tcgcgcagac tttcttccgg ctgcaattgt ttaaccagtg
     5041 tacgagttct gccatcggaa agttgcttgg aaacatgaaa atgagcgtca taataagccg
     5101 caagctgggg cagatgagta atgcataata tctgatgatt gcgggaaagc agccatagtt
     5161 tttccccgac aatcgaaccg acccgcccgc cgattccctg gtcgatttca tcgaaaatca
     5221 tcgacgggat cgaatccgct tcggccaatg tgtttttcaa cgccaacatc agtcgcgaag
     5281 tctccccgcc ggatgcgatc ttggccaacg gtttcaatcc ttcgcccggg ttcggcgcaa
     5341 tcagaaattc aacctgatca aagccgttct catcataagc caatgatctg ccgtgccggt
     5401 ctcggagtcc gttttcagcc gccgtttgcg taaatcggat ctcaaactgt gccgattcca
     5461 ttcgcagctc atgcagctcc ttttccacgc tctcgctcac tttccgagaa gcttcatgcc
     5521 ggcggtcgga aagctcctgc gccaattcgg ataacaggtt gatcaccgaa gtttcctgcc
     5581 ggatcaaatc ttccgtttga tccgaaatat tttcaagccc agccagctgt tcttccgccc
     5641 gcgtggcaaa ttccaaaacc gcatgcaggc tgccgccgta ttttcttttt aaattgttca
     5701 atagcgccag acgttcttcg atggcatcca gccgggcggg gttgaattcc aacgtttcct
     5761 gataacgctg taaagaggcg agcaattcgt tcagcgcatc atttatcgcg gcgacttctt
     5821 cgccgagcgg cgccagctcc ggatcgattc ggcttaaatt atccattgat ttggataaat
     5881 aacccagtaa ttcgaaaatt cccggatatt cttccgatct tccttccagt tgctccatgg
     5941 ctttttgagt cagttggctg agattctcgg cattcgccag ccgatcccga tctttcttca
     6001 gcgtttcttc ttccgattca ttgattttgg ccgagcgaat ctcattcatt tgaaaggaca
     6061 acatatcttt ttttcgcagc aattcttcgt cattcatgcg aatctcggtc aatcgggtac
     6121 gaattttctg taattttttg aattctgctt gatatttctt cagcaattcc tgatttccgg
     6181 caaagcgatc cagcaagacg atatgattgg aaggattcag cagtgacaga tgatcggatt
     6241 gaccgtgaat atctaccagc agccggccga tttcccgcag cagattaacg ttcacactgc
     6301 tgccgttgac ccgtgccgag ctgcggccgc cggcccgaat atcgcggctc aagaggatct
     6361 gatcgccgtc cgaatcttcg ggcaaatctt cccttttcag tatttcccga atcgcctccc
     6421 gattctcgtc cggccggaaa accgcttcga caatcgcccg atccgtccct tcacggataa
     6481 aagtcggatc ggtcttcccg ccgacaaccg ttgaaacggc atccaaaata atcgatttac
     6541 cggcgccggt ctcacccgtg aagatattca aaccttcctt cagagaaagt tccagctcat
     6601 cgataatcgc aaaatttcgg atatataatt cggtcaacat ttcagcgtct gcgaatggca
     6661 aaaccgcgca tattcgttaa tatggtcgta cataacaaag caatgtacaa aattccccaa
     6721 ccgacggaca gggtatatcc catcaaaccg gcgtaatttc cgatgaatat catccggaaa
     6781 agccccaaga tatatcgcag aatcggaacc gctccgccga ttccggccgc gattgccgtc
     6841 aatttattca gatccgccgc tcggcgtttt tgagtaaccg cccggacggc tttggcgatc
     6901 agattcccca acaacaatcc cagaatgatc gccgtcagca tggaataaaa attaaaaaaa
     6961 tacaaaacaa aagatcccac ggctccgatc agaaaagcaa tgatcgccga taaaacataa
     7021 tcccgggata cggcggtgtt gaattttttc tgctgctctt tgacacattc cttacagcga
     7081 taaccggtcg gtgtggagac cgcgcaatcc atgcaaatag gccgcccgca ttgattacaa
     7141 caaatcaatg tctcccgatt cggatgcttg taacaggttt taatttcgct tatttcatca
     7201 ctcatattgt tgtctttcga ttggcgctcg gatttcgttg catatattga ataaaattcg
     7261 agtagaaaaa tccctttcct tccaagcgca cataacgcgc cgaaaattct gaagcggaga
     7321 ccgaaatcgt atccgattca tcaatcatca tcccatcttg cccgtccggt gaaaaaatcg
     7381 tttcatgttc ggaagacgat cgaatttcca ccacatcgtt ttcgtcgaga acaatcccgc
     7441 gctccaggca caaatggggc gccacgggga tcaccagaat atttcgcaat tccggttcca
     7501 taatcggtcc cccggctgcc atggcgtatg cggtggaccc cgttgcagtg gaaacaatca
     7561 atccgtccgc gacataatcc gtcagataat tttcattaat gcgaacattg atacgaatcg
     7621 ggcgtaattt ggaaccatga ccgatcaccg catcattcag cacttcccag cgagttcgtt
     7681 cgttatcctt tcgaaccagc tcgacatgca acatcatccg tttttcgatc cgccaatccc
     7741 cgttcattaa agccggaagt ttttcccgcc attgattttt ggcgatttcc atcataaaac
     7801 cgaagctgcc catattgatc ccgcagaccg gaacctcaaa tggtgcgcat agtttattgg
     7861 tgcgtaacat cgtcccgtca ccgccgaaag taatcaccaa atcaaagggg cggcgttcaa
     7921 ggctttcacg aaaagcttca tcgtaaaggg accaaccttc cgcgacagcc tgtccgtgaa
     7981 aacgggtatt gatttcttcg ataatcttct tggcttcttc ttcggcggca gccagatgag
     8041 ggtgccaggt cacaccgatt cgttcgggtt tttttgtcgt ttgattcatc ttcggctcac
     8101 aatttttcat cacaaatcga tcggtatccg caacccccgc agcggaatga atcgcaatga
     8161 gaacgatgaa cttcttcgag cgaatcatct tccctgatcc tttccaaccg gtccagaagt
     8221 tcatcccttc tttttattgt atacgaaatc ttaaactctt tgtttcgata acgcaaaatt
     8281 ccgtaatccg gctttacggc aaaggccttt tccgtcaaca ggcaataagc cattaattgg
     8341 aagacgtgag aagccggcgg tgtttccgga gccggctgag atttgatttc gatcggaatc
     8401 agttgttccc ctttttgaat caacagatcc ggtttccccg ccaagcgggt ttccggatca
     8461 aacagagggg ctttcaactt cccgttcgga gcgatatcct cataaaccgg tttgccttga
     8521 aagagaacat cggaagcttt ctttcgctcc gtcaaaatca acccggccag cgcaagcgcc
     8581 aaaagcaaaa ttccgaggag cagctgctga ttcatctcgc ccccacgttc gccgcaatcc
     8641 accaggaaat caagcccagc gccaaaatca gcatcaggat caggataatc cgggcaatcc
     8701 gcatcgccga taatcgcctg cccattttcc gatgaaaacg ggtgccttca ttcatggcag
     8761 gcaggttttc gttgggagcg tttcgattcc
//
LOCUS       DAOMIV010000018         8718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8127_length_8718_cov_5.257301, whole genome shotgun sequence.
ACCESSION   DAOMIV010000018 DAOMIV010000000
VERSION     DAOMIV010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8718
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8127_length_8718_cov_5.257301"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKV59_00945"
     CDS             <1..221
                     /locus_tag="PKV59_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45346.1"
                     /translation="GLSSDSPLGFLVLSRGNETHLQNWHWITVTGTDITEDAILATAS
                     DEGKKITFDLSLWYHTTSMNGGLVYFTA"
     gene            397..1410
                     /locus_tag="PKV59_00950"
     CDS             397..1410
                     /locus_tag="PKV59_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP45347.1"
                     /translation="MTVTIRDIAKKAGVSPSTVSRVINGKSVISNETRNKIFAVMEEL
                     DFHPNSLARNLANGSSRALALVINAEDKKSFSNQYFTHSVFGIEQIAQTNGYNLIITH
                     NQSKGMTPIEQLIYEKKADGIILPPSLITISLLKKLLNQKFPFVVLGEPPFMRNEINW
                     VDINNAQGSESAVNHLLEKGYRSIVFVGENQQQIFTKNRIAGFRDALEKQGIEPRQDF
                     IIESDGTQEAAYRIAAAFLQKTDPPDAFLCSDNTIAFGILKAVKSLGRAIPQEIGIVT
                     FDNYPLAQYTEPSLTSIDVDTYLLGEHAAFVLLKTIENETNIQHILVGTKLFQRESTD
                     RKL"
     gene            1407..2774
                     /locus_tag="PKV59_00955"
     CDS             1407..2774
                     /locus_tag="PKV59_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HQP45348.1"
                     /translation="MNQAAIFYRATREFILPVSRQDLFIRLTAARSDIRECRVIFWKR
                     NQTDAASVRFSDMKVAYRDAVHDDFRTVISFPEPVHYIKYYFELIDFAGDIRFLSENG
                     LSDEKPADGFFEFLYVNTADVFSVPEWAKGIIYYQIFPDRFYRSQNAPESSRLVHWNS
                     FPEQDNYFGGDLRGIIEKFDYLKELGAECIYLTPIFKAEFNHKYATTDYFTVDPAFGT
                     LTDLQDLVKLAHSNGIKIILDGVFNHVGRSFPQFSDLLDRGKNSSYKEWFYPSDFPLT
                     SNPLNYECVGDYPYMPKLRTAHPDVRNFILSVMIYWIEQTGIDGWRLDVADEMEMSTL
                     QTIRMVLKEKYPDILLLGETWGDAFRLVGNGDQMDSAMNYLFRNAVLDWIAKGKIRAT
                     DFDRRINQLLSKYNDLTNSVMYNLIGSHDTARFLSEAGENKNKLKLRSFPNDILRFTR
                     RLLWG"
     gene            2734..3207
                     /locus_tag="PKV59_00960"
     CDS             2734..3207
                     /locus_tag="PKV59_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HQP45349.1"
                     /translation="MTFCGSPAVYYGDEIGMSGENDPGCRAGMIWDQESQDRDLFQWY
                     RCFIELRKAHPALRKGTFRSNFCDDEKNIYGFIRQTSDEALFVLMNNSENCSEIILPV
                     PDRDAEYRELFSSMIRRPEPLQDGITFYNQEITEYQGQLSIPIDAYSVQIYKKEK"
     gene            3209..4471
                     /locus_tag="PKV59_00965"
     CDS             3209..4471
                     /locus_tag="PKV59_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQP45350.1"
                     /translation="MNEKKKILAIMLAVVLLSLSAIAAGAMSPENQEEITIDFWHHYS
                     AQSAENETLVNVLIPKFESENPGIKVNAVSHEWAELHNKILISAGTGSLPDIARCDIA
                     WLPEFQKMGVLVALDQEMSDFTGVSGKLLESAMSTAKIQDHYYGLGLNTNTKILFYNE
                     KMLKDAGVAVPATMDELISAIKALSGTNADGQQVWGWNEPGLYGWNLCPFIWSFGGSL
                     TNEDQTIATGYINSPATVKAIDTFVSLYKEGALTGFNSGDIPMTDGFGTNRYAMMLEG
                     PWKIAELQGAYPDLAYGTAPMPAGEGGSVSVLGGEDIAMFNTAKKEAAWKFMQFMTGE
                     FAQIEMAKCGQIPVNQSALEDATVKNAAFAPFLEAIKTAKARPTVAAWSEMDNELNVA
                     VTAIINGEKTTQQALDDLAGVWDQLLTQ"
     gene            4569..5471
                     /locus_tag="PKV59_00970"
     CDS             4569..5471
                     /locus_tag="PKV59_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP45351.1"
                     /translation="MKTKSNKNKIQIFLLLLPGLAVFCFFTVYPIIKLFLMSFSKWDF
                     TSMRQQEFIGLQNYREVLADKFFWTSFTNTIVYTLVTVPLQMILGLFVAILVNGIKKF
                     QVTFRVLNYLPVITSWVIASLIFRYVFNTEGLLNYFLSQVLHVTASNIRWLDSRWSGL
                     TVAMILGIWKGIGWNMVIFLAALQQVPIDLYEAAAIDGCNGIQKFFKITLPGIKGTIL
                     FVWVMLTIGGFNVFTSIKMITGGNPAHQTDTILTWMYYKAFSTGKFGYSAAVSFIMAF
                     TLGIMAIFQFKAMRNRDRELEEIG"
     gene            5468..6307
                     /locus_tag="PKV59_00975"
     CDS             5468..6307
                     /locus_tag="PKV59_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP45352.1"
                     /translation="MKEKKSVIFFRYALLTIGVILSCLPMIYMISTSLRPNGALYEYP
                     PRFFPKLQDVTVENYQYIISQNKFYINFFNSVIVSVSTVALAAIISSGLAFVIARFQF
                     PGRKVLFGFIIVTMIIPGTTLIVPQFELAVKLKVVNKLWGLIPFYTAWVIPFSTFMIK
                     GFMENIPDEIIEAATIDGADIFTVFFRIAIPLSTPAIASVSIFNFLTAWEEYPWANTV
                     INDNAIRTLPIAIAGFFGQHQFTQWGYVFAMSALSLIPVILVFILFQKYFVTGLTAGS
                     VKG"
     gene            complement(6515..7231)
                     /locus_tag="PKV59_00980"
     CDS             complement(6515..7231)
                     /locus_tag="PKV59_00980"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQP45353.1"
                     /translation="MKLICYLSNGYPSIEESIKMAEIYTHAGADMIEIDFPSRNPYLE
                     SEFIADRMAEALKACDDYDAYMDGMKRVKRLLPSTQFILMVYENTVEEIGPDKFIDFC
                     RSNHYEDLILVGLKDESIKNLCIRNGLKVSCYVQRRMDRMEIEYAKQSNGFVYLQAKA
                     DPDQVNPLYPTLKDCIDHLRCIGIDRPIYCGVGIHTPEDAKAAKEAGADGVFVGSTIL
                     KLHNDIPALIKEINEFKKNC"
     gene            complement(7235..8389)
                     /locus_tag="PKV59_00985"
     CDS             complement(7235..8389)
                     /locus_tag="PKV59_00985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQP45354.1"
                     /translation="MMRPMKLAGSELMFGRGCLEYIHKIPEKRVFIVIGGSSMEKNGI
                     LAKVENLFHDNGAETAVFRGVEPDPSFKTVLKGAAEMLKFEPDLIVGLGGGSVMDAGK
                     AMWVCYEHPEIQTLDQLLTANPFPKLRQKARYLCIPTTAGTASEVSRSVVITDDEKGL
                     KHGLGNMEMMPDIAIDDPEVTIFLPPHITAETGMDALTHALEALVSNRANYLSDILAV
                     PAALDIIRTLPIAYANGKDMDAREMMLNASMTAGLAFTNVSLGIVHSLAQTIGGYFHI
                     SHGLADAIILPYIIRYNAAVPYADKKYRDLAAKFGKTDLSEIVDDLNRRLHIPSTLRE
                     PLQEEARFESLIPEMAVTAKNDGCTKTNPIIPDVNEFAELLKRCYYGEEK"
     gene            complement(8405..>8718)
                     /locus_tag="PKV59_00990"
     CDS             complement(8405..>8718)
                     /locus_tag="PKV59_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HQP45355.1"
                     /translation="THMLMKDISIEQSAKDAAACGADGLIVTGTATGGETPLEMIRRV
                     KEVVDIPVLAGSGVNSRNIKEQMSVADGAIVGTALKEDGILKNPISFELTSELMKSLR
                     K"
BASE COUNT         2401 a         1973 c         1903 g         2441 t
ORIGIN      
        1 aagggttatc ttcagacagt cctctgggat ttttggttct ttcccgcgga aacgagacgc
       61 atctgcaaaa ttggcattgg ataaccgtca ccggtacgga tattacggag gatgccatcc
      121 tggctaccgc ttccgatgaa ggaaagaaaa taacctttga tttatctctt tggtaccata
      181 caacctcaat gaacggcggt ttggtttatt tcactgccta acatgatttc cgatctgttc
      241 gtaatatgac atttctcact attgacaaat ggcaggtgat tgtctattat ttttaatgca
      301 accggttgca ttatttatta aagattttag aaaagcaaca aatctttatg agtaaattct
      361 gcaaaataat catacaaccg tttgcattga ggatcaatga ctgttaccat tcgcgatata
      421 gccaaaaagg ccggcgtttc cccttccacc gtttctcgag ttattaatgg aaagtccgta
      481 atttcaaatg agacgcgtaa taagattttt gcggtgatgg aagaactgga ttttcacccc
      541 aacagccttg cgaggaatct ggctaatggg agttccagag cgctcgcgtt ggttattaac
      601 gccgaggata aaaaatcttt ctcgaaccaa tatttcactc acagcgtttt cggaatcgaa
      661 cagattgccc agaccaacgg atataacctg atcatcacgc acaaccaatc caaagggatg
      721 acaccgatcg aacaactgat ctacgaaaaa aaagccgacg gaatcatttt gccgccttct
      781 ttgatcacca tatcattact gaaaaaactc ttaaatcaaa aattcccttt tgtggttctc
      841 ggagaaccgc cttttatgcg gaacgagatt aattgggtcg atattaataa tgcgcaaggc
      901 agcgaatccg ctgttaatca tttgttggaa aaaggatacc ggtccatcgt ttttgtcgga
      961 gaaaaccaac agcagatttt taccaaaaac agaatagccg gctttcgtga tgctttggaa
     1021 aagcaaggga ttgaacctcg acaagacttt atcatcgaat cggacggaac tcaggaagca
     1081 gcctatcgga tcgccgcagc ttttttacaa aaaacggacc cgcccgatgc gtttttgtgc
     1141 agtgacaata cgatcgcttt cggaattttg aaagcagtta aatcattggg gcgggccatt
     1201 ccgcaggaaa tcgggattgt cacttttgat aattatccgt tagcacaata taccgaacca
     1261 tctctgactt ccattgacgt tgacacctat cttttgggcg aacatgcggc ttttgtatta
     1321 ttaaaaacaa tcgaaaacga aacaaatatt cagcatatat tggtcggaac caaactattt
     1381 caacgcgaat cgacggacag aaaattatga atcaagcggc tattttttat cgggcaaccc
     1441 gagaatttat tcttcccgtt tccaggcaag atctttttat tcgcctgaca gccgccagaa
     1501 gtgacattcg agaatgccgg gtcatttttt ggaagcgaaa tcaaacagac gccgcttccg
     1561 tccgcttcag tgacatgaaa gttgcttatc gcgacgccgt ccatgacgat ttccggaccg
     1621 tcatctcttt cccggaaccg gtgcattaca taaaatacta tttcgagttg atcgattttg
     1681 cgggggatat ccgcttttta tccgaaaacg gattgagtga cgaaaagcct gcggatggtt
     1741 ttttcgaatt tttgtatgtg aataccgcgg acgtgttctc tgtccccgaa tgggcaaaag
     1801 gcatcatcta ttaccaaata tttcccgatc gattttatcg cagtcagaac gcccccgaat
     1861 cctcccgttt ggttcattgg aacagttttc ctgagcaaga taattatttc ggcggggatt
     1921 tgcgtggaat tatcgaaaaa ttcgattacc tgaaagagtt gggagcggaa tgtatctatc
     1981 tgacaccgat tttcaaagcc gaattcaatc ataaatatgc gacaacggat tatttcaccg
     2041 tcgatccggc tttcggaacg ttaaccgacc tgcaagattt ggtcaaatta gcccattcga
     2101 acggaataaa aataatattg gacggggtgt ttaatcatgt gggcagaagc ttcccgcaat
     2161 tctccgatct gctggatcgg ggaaaaaatt catcctacaa ggaatggttt tatccctccg
     2221 attttcctct tacttccaac ccattaaact atgaatgcgt cggtgattat ccgtatatgc
     2281 ccaagttgcg gaccgctcat ccggatgtgc gaaattttat tctttcggtg atgatttatt
     2341 ggatcgaaca aacgggaatc gacggttggc gattggatgt agcggatgaa atggaaatga
     2401 gcacccttca gactatccga atggtgttaa aagaaaaata tcctgatatt ctattactcg
     2461 gtgaaacctg gggagatgca tttcggttgg tcggtaacgg tgatcaaatg gacagcgcca
     2521 tgaattacct ttttcgaaat gccgttttgg attggatcgc caaggggaag atccgtgcga
     2581 ctgattttga ccggcggatc aatcaactgc tgtcgaaata caacgacctc accaattcgg
     2641 taatgtacaa cctgatcggc agccatgata ccgcccgctt tctttccgaa gccggagaaa
     2701 ataaaaacaa attgaaactc cgcagctttc caaatgacat tctgcggttc acccgccgtt
     2761 tactatgggg atgaaatcgg aatgtcgggc gaaaatgatc cggggtgccg ggcgggtatg
     2821 atttgggatc aggaatctca ggatagagat ttgttccaat ggtatcgttg tttcatcgaa
     2881 ctacggaaag ctcaccccgc tctacgcaaa ggaaccttcc gctcaaactt ttgtgatgac
     2941 gaaaaaaata tttatggatt tatacggcaa acatcggacg aagcactgtt tgttcttatg
     3001 aataacagtg agaattgttc ggaaattatc cttccggttc cggatcggga tgctgaatat
     3061 cgggaattat tttcttcgat gatccgccgg cccgaaccgc tccaagacgg aatcactttt
     3121 tacaatcagg aaataacgga atatcaggga cagctatcga ttccgattga tgcctattcc
     3181 gtacaaatat ataaaaagga gaaatgatat gaacgagaaa aagaaaattc tggcgatcat
     3241 gctcgcagtt gtcttattaa gcctgtcagc tatcgcagcc ggtgcaatgt cgccagaaaa
     3301 ccaagaagaa atcacgattg atttctggca ccattatagc gcacagtccg ccgagaacga
     3361 aacattggtc aatgttttga ttccgaagtt tgaaagtgaa aaccccggaa tcaaggtcaa
     3421 tgcggtttca cacgagtggg ctgaactgca caataaaatc ctgatcagtg ctggtaccgg
     3481 aagccttccg gatatcgcgc gctgcgatat cgcctggctg cccgaattcc aaaagatggg
     3541 cgtattggtc gctctggatc aggaaatgtc tgatttcacc ggtgtttccg gaaaactgct
     3601 tgaaagcgcc atgagcacgg caaaaataca ggatcattat tacggcttgg gattgaatac
     3661 caataccaaa atcctcttct ataacgaaaa aatgctgaaa gatgccggag tagccgtacc
     3721 ggccactatg gatgaattaa tatccgcaat caaagcgctc tcaggcacca atgcggacgg
     3781 gcagcaggtc tggggatgga atgaacccgg gctttacggt tggaaccttt gcccgtttat
     3841 ttggtcattc ggaggttcgc tgacaaatga ggaccaaacc atcgcaaccg gttatatcaa
     3901 cagtccggcg acggtaaaag cgattgatac cttcgtttct ttatacaaag aaggagcttt
     3961 gaccggattc aacagcggag atattccgat gaccgacgga ttcggcacca atcgatatgc
     4021 catgatgctg gaaggacctt ggaaaatcgc cgagcttcag ggcgcttatc cggacttggc
     4081 atacggtacc gctccgatgc cggcagggga aggcggatcg gtctctgtct tgggcggtga
     4141 ggatattgcc atgttcaaca cagccaaaaa agaagccgca tggaaattca tgcaattcat
     4201 gaccggtgaa tttgcccaaa tcgaaatggc aaaatgcggc cagattccgg tcaatcagtc
     4261 agcattggaa gatgcaaccg tcaaaaatgc cgcgtttgct ccttttttgg aagcgatcaa
     4321 gaccgcaaaa gccagaccga cggtggcagc ttggtccgaa atggataatg aactaaatgt
     4381 tgcggtaacg gcaattatta atggcgaaaa aacaacgcag caagcgttgg atgatctggc
     4441 cggtgtttgg gatcaattac tgacccaata aaaaagtttt taactcaaat caaacgagtg
     4501 aacgtgtcag agctgatcga ttctgacacg ttcactcaag gcggcgtcga atcggagttg
     4561 cggaaaaaat gaaaaccaaa tcaaacaaaa ataaaatcca aattttcctg ctgctgttac
     4621 ccggtttggc ggttttttgt ttttttaccg tttatccgat tatcaagctt tttctgatga
     4681 gcttcagtaa atgggatttc acttccatgc ggcaacaaga atttatcggc ctgcagaatt
     4741 atcgtgaagt tttggctgat aaatttttct ggacatcttt cacaaatacg atcgtctata
     4801 cactggtgac cgtaccttta caaatgatac tggggctctt cgtggcgata ttggtaaacg
     4861 gcattaaaaa gtttcaggta accttccggg ttttgaatta tttaccggtt atcacctctt
     4921 gggttatcgc ctccctgatt tttcgttatg tatttaatac ggaaggactc ttaaattatt
     4981 ttctttctca ggttttgcat gtcaccgcct ctaatatccg ttggctcgat tcccgttggt
     5041 ccggattaac ggttgcgatg attctcggaa tatggaaagg aatcgggtgg aacatggtaa
     5101 tttttctggc cgctttacaa caggttccga ttgatttata tgaagcggca gcaatcgatg
     5161 gttgcaacgg gattcaaaaa ttttttaaaa tcaccctgcc cggaattaaa ggaacgatcc
     5221 tttttgtttg ggtcatgctt acaatcggcg gtttcaatgt atttacttcc atcaaaatga
     5281 taaccggcgg gaatccggct catcaaacgg ataccatttt gacatggatg tattacaaag
     5341 ctttcagcac cggtaaattc ggatattcag cagccgtatc gttcattatg gcctttacac
     5401 tcggaattat ggcaattttt caattcaaag ctatgcgcaa tcgcgacagg gaattggagg
     5461 aaatcggatg aaagaaaaaa agagcgtcat cttttttcgg tatgccttat tgacaatcgg
     5521 agtgattctt tcttgcttac cgatgattta tatgatcagc acctctctgc gtcccaacgg
     5581 tgcactttat gaatatccgc cccgattttt ccccaagtta caggatgtaa ctgttgaaaa
     5641 ttatcaatat atcatcagcc agaataagtt ctatatcaat tttttcaaca gtgtcatcgt
     5701 gtcggtatcg accgtcgcgc ttgccgccat catttcttcc ggtttggctt tcgtgatcgc
     5761 tcgatttcaa tttccgggtc gtaaagtttt gttcggtttt attatcgtca caatgattat
     5821 tcccggaacc acattaattg tcccccagtt cgaacttgcg gtaaaactga aggtcgtcaa
     5881 taaactttgg ggattaattc ctttttacac ggcatgggtc atcccttttt ccacttttat
     5941 gatcaaaggg ttcatggaaa acattcccga cgaaatcatc gaagctgcga ctatcgacgg
     6001 agcggatatt tttaccgtct ttttcagaat cgccatcccg ctttcgacac cggctatcgc
     6061 gtctgtttcc atttttaatt tcctgaccgc ttgggaagaa tatccctggg caaatacagt
     6121 gatcaacgat aacgccattc ggactttacc gattgcgatc gccggcttct tcggccagca
     6181 tcaatttacg caatggggat acgtatttgc catgtccgca ttaagtttga tcccggtaat
     6241 cttggtcttt atcctgttcc aaaaatattt tgtgacggga ttaaccgccg gaagcgtcaa
     6301 gggataatag aatgaaagtt gcgttgcctt ccagcgataa aacaaccggt tgaaactttg
     6361 cgcagtgatc ggaccgattt cttaagattc ctcgattaaa gtaccgacaa caaaggacgg
     6421 aatcaatcaa aaaggccgcc gaagcgacct ttttgacccg acaaaaaaat gccttcttca
     6481 aatgaaatga taaaaataaa taaaaattga aggatcagca atttttttta aattcattga
     6541 tttcctttat caaagccgga atatcattat gaagtttcag aattgtgctg ccgacaaaga
     6601 caccgtcagc gccggcttct tttgccgcct tggcatcttc cggtgtatga attccgacac
     6661 cgcaataaat cggacgatcg ataccaatgc aacgtaaatg atcgatgcaa tcttttaaag
     6721 tcgggtacag cggattaacc tgatccggat cagcttttgc ctgcaaatat acaaaaccgt
     6781 tggattgttt ggcatattcg atttccattc ggtccattct gcgctgaaca taacaactga
     6841 cttttaatcc gtttcggata cagagattct taatactttc atctttcaga cccaccaaaa
     6901 tgagatcctc gtaatggttg gatcgacaga aatcgatgaa tttatcggga ccgatttctt
     6961 cgacggtgtt ttcatagacc attaaaataa attgtgtcga cgggagcaac cgttttaccc
     7021 gcttcatacc gtccatgtaa gcatcataat cgtcgcaggc tttgagcgct tcggccattc
     7081 gatcggcaat aaattcactt tccaaatagg gattacggct cggaaaatca atttcaatca
     7141 tatccgcgcc ggcatgggta taaatctcag ccattttgat gctctcttca atggagggat
     7201 acccgttgga tagatagcaa attaatttca tgatttattt ctcttcaccg taatagcagc
     7261 gtttcagcaa ttcggcaaat tcatttacgt cgggtatgat cggattggtc tttgtgcatc
     7321 cgtcattttt cgcagtgaca gccatttccg gaatcagaga ttcaaagcgc gcctcttctt
     7381 gcaagggttc ccgcagcgta gacggaatat gcagccggcg gttcaaatcg tcaacaattt
     7441 cggaaagatc cgttttcccg aatttcgcgg caaggtcacg atatttctta tccgcataag
     7501 ggacggcagc gttatagcga atgatatagg ggaggatgat cgcatcggca agcccgtgag
     7561 aaatatgaaa atagcccccg attgtttgag ccaatgagtg aacaattccc aaagacacat
     7621 tggtgaaagc caaaccggct gtcattgagg cattcagcat catttctcgc gcatccatat
     7681 cctttccgtt cgcgtatgcg atcggaagcg ttcgaatgat atccaaagcc gccggaacag
     7741 ccagaatatc actcagataa tttgcccgat tggagacaag tgcttccagc gcatgagtca
     7801 aagcgtccat tcccgtttca gccgtaatgt gaggtggcag aaagatggtc acttccggat
     7861 catcgattgc gatatccggc atcatttcca tatttccgag cccgtgtttc agcccttttt
     7921 cgtcatcggt aatcacgacg gaacgagaaa cttcacttgc ggtgccggct gttgtcggaa
     7981 tacacaaata acgtgctttc tgacgcaact tcggaaaagg attggccgtc aacagctgat
     8041 ccaacgtttg aatttcagga tgttcgtaac aaacccacat ggcttttccg gcatccatca
     8101 cggatccgcc tcccaagccg acaatcaaat ccggctcaaa tttcagcatt tccgccgcac
     8161 ctttaagcac tgttttaaat gacggatcgg gttccactcc tcgaaaaacg gcggtctcgg
     8221 ctccgttatc atgaaaaagg ttttccactt ttgcaagaat gccgtttttc tccatactgg
     8281 acccgcctat tacaataaaa acccgctttt ccggtatctt gtggatatac tcaaggcaac
     8341 ctcttccgaa cattaattcg ctgcccgcta atttcattgg tcgcatcatg atctctattt
     8401 ccctttattt tcgtaaactt ttcattaatt ccgatgtcaa ttcgaatgaa atcgggtttt
     8461 tcaatattcc gtcttcttta agagcggttc cgacaatcgc cccatccgca acactcatct
     8521 gttctttgat atttcggctg ttcacgccgc ttcccgccaa aaccggaatg tcgacgactt
     8581 ccttcacgcg gcggatcatc tcaagcggtg tttctccgcc tgtagcggta ccggtaacta
     8641 ttaagccgtc cgccccacac gctgcggcat cttttgcgga ttgttcaata gaaatatcct
     8701 tcatcagcat atgagtat
//
LOCUS       DAOMIV010000019         8586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8275_length_8586_cov_4.655609, whole genome shotgun sequence.
ACCESSION   DAOMIV010000019 DAOMIV010000000
VERSION     DAOMIV010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8275_length_8586_cov_4.655609"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKV59_00995"
     CDS             <1..493
                     /locus_tag="PKV59_00995"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQP45356.1"
                     /translation="GLWLFCRKYGFKISKFTDTISPGLALGQAIGRLGNYFNQELYGA
                     PSDLPWAITIDPQYRIPGFEQQATYHPLFLYELIYNLLNMGFLLWVDHRFGKRLKDGD
                     IFLIYLIVYPVGRFFLEFLRLDTAMVGGLDMNQLIMGVTAVCATAGLIIRHTFQPKRD
                     LTK"
     gene            630..896
                     /gene="rpsT"
                     /locus_tag="PKV59_01000"
     CDS             630..896
                     /gene="rpsT"
                     /locus_tag="PKV59_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQP45357.1"
                     /translation="MANIKSQIKRNRQNEKRRIQNRVVRGSTRTALYKARLAVETNQE
                     NSEEMVKKAIIALDKAAQKGVIHKNNAARKKSRLIKKLTATKKA"
     gene            1051..2811
                     /gene="argS"
                     /locus_tag="PKV59_01005"
     CDS             1051..2811
                     /gene="argS"
                     /locus_tag="PKV59_01005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQP45358.1"
                     /translation="MFEKEQQLIEDQIRKICEAHNYPVKSLTWSWIPFSGNWGISTSF
                     FQVAADDARSRGQKINVGLHAQEIAQMTADSLELPEGFERAEAVRGYLNLYFTTRIYS
                     QRVIDTVLEEKDNFGRGQRNGERVMVEFSQPNTHKAFHVGHLRNVILGNAVCNILDFA
                     GNDVIRANYLGDIGLHVIKWLWCYLKYHNGEKPEGDITRWMGDIYAEASRRHEEEPEA
                     ESEIRELFARWDRKDPEIVELWRVTRQWSLDGFEQIYKTLNVRFDKIYFESDVEDSGK
                     EIVKELIAKGIAVDERPENAVVVKLDELCGTKDKYRSLVVLRSDGTSLYATKDLSLAI
                     RKFEEFHLTRSIYVIDVRQTLYLQQIFKTLELMGYSWAKNCYHLAYEIVNLPGNVTMS
                     SRDGTVVFLQDLIDEAKSRALAIVQEKNPSLTDAEKAEISEKVALGALKYSMLSRDNA
                     KIVTFDWEAALDFNGQAAPYIQYAAVRANSILRKSENGLPDQTALSYELTPVEVELID
                     LISRVPREIQRSAAECKPLFISNLAFDLAQAFNNFYHQCPVLQAEPPVRDYRLRLAAA
                     ARQTMINCLALLGIDSPEIM"
     gene            complement(2862..4139)
                     /gene="serS"
                     /locus_tag="PKV59_01010"
     CDS             complement(2862..4139)
                     /gene="serS"
                     /locus_tag="PKV59_01010"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQP45359.1"
                     /translation="MLDMNLIRENPELVIKSMKDRQMDPAPVNRVIELDTARRKLLSE
                     SEKLKAERNATSKEIGRIKDTEERNAKIEAMRLVGDKIAELDKKIKETEEELNSLIMI
                     IPNIPDPRVPYGVSDAENVVVRYEGKIAEREFDPIPHWDLGPALGIIDFDRGVKLTGS
                     RFYILNGAGARLQRALIAFMLDLHTRQGYEERYTPFMVKSEILYGSGQLPKFRDNLYH
                     DAEEDFWMVPTAEVPLTGLYMNEIIEEDSLPMRFTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYCYSHPDKSADELDRMLNAAEETCRQLDLPYRIVAQCTGDLGFNARICYD
                     IEVWAPGCKEWLEVSSVSDDGDFQSRRGAIRFRDKESGKTRFLHTLNGSGLGLPRTLI
                     AVLETYQQAGGSVVVPEVLKPWMGGINVIEKQQ"
     gene            4201..4326
                     /locus_tag="PKV59_01015"
     CDS             4201..4326
                     /locus_tag="PKV59_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45360.1"
                     /translation="MVPPAFAQPRASLSGDNDPESAFLSHHCFSKAVRIFYARGG"
     gene            complement(4466..5203)
                     /locus_tag="PKV59_01020"
     CDS             complement(4466..5203)
                     /locus_tag="PKV59_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQP45361.1"
                     /translation="MDQLQDKIILIEGKQTDHPSFFLSLKKRGYNVEIASTGNSAIEK
                     IQESDPMIVLIDAASMRTASNRIVYRIKKRNPDLPIILIIDEMLKQKKISDKADLVLC
                     LPFTVQKLLNRIMLFAPAKKSTLKCAGDLALDTSRNCIYIDNRVVGVTPRVSKLLNLF
                     MDRPGEIIKREELFRELWDTDYLEDMRSLDVHIQWLRKAIEENPKNPQYLITVKGQGY
                     MLEPNGNGKNKKIDQCALSPVCCEKES"
     gene            5506..6135
                     /locus_tag="PKV59_01025"
     CDS             5506..6135
                     /locus_tag="PKV59_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HQP45362.1"
                     /translation="MSELFEVTVDSIRVSLTTQQRVLILHEPKNELYLPIWIGSFEAE
                     SIVIALQGIEVSRPQPHDLLKNVIAALDGKLRNVVLSKIQDDTFYSFLVLESQGNEIL
                     VDSRPSDAISLAIRCKVPIFVDKAVLLSAGIQPEKDIRELNSAKKNFAAEPDQEEFVD
                     DAKLNIFDDFLKNIDLPDSGETGKSDRSGKQDNEKKDTDSNSDDLLFPI"
     gene            6139..7536
                     /gene="dnaB"
                     /locus_tag="PKV59_01030"
     CDS             6139..7536
                     /gene="dnaB"
                     /locus_tag="PKV59_01030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQP45363.1"
                     /translation="MEINGPYPTENFTPEVSESDLRLQPHSREAEEAVLGAILINSEK
                     FYDVAQIISANDFYIHRCRWIWEAFAALLEKGQDIDVQTVANELEKNGKLTEAGGKLY
                     LYSLASNTPSSMHAESYAKIIAEKSVRRQILTAASQMQQYAYDESKSLDTILDNSEKA
                     IFNVSEKRTRNEVQPIRSVVGTIYDEVRLLARQDQDIVGVPTGFKDLDRLLGGMQKSD
                     LIIVAGRPGMGKTGFLLSVLKNAALNYHMHVAMFSLEMSNEQLAIRLIAQQTGINSQK
                     IRSGKLAEDELPMFIDALDVLGTTTVFLDDTPAITPLQLRSKCRRLDLEHHLDLVIID
                     YLQLMSTDSRNENRVQEVSYISRNLKILARELNVPVLTAAQLSRSVEQRSEKIPILSD
                     LRESGSLEQDADVVMFLHNPNTDDSKTVGLDGAMKLVVAKHRNGPVGDIDLVFVKKLA
                     RFENAARISDSGGIR"
     gene            7533..8147
                     /locus_tag="PKV59_01035"
     CDS             7533..8147
                     /locus_tag="PKV59_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HQP45364.1"
                     /translation="MSFSGFNTGIEVIPTQFFEEILPEIDSMTELKVSLFAIHLLNQF
                     EGDQRYILYSDFTDAAEFMDGLAGIEEDREDALKDGLEKAVLRGTLLKVEYGGESLYF
                     LNSPKGRTAVENLEKGKWKPDAFLHLSGTLNLFRPNIFQLYEENIGPLTPLMADRLKD
                     AEQMYSEEWITEAIDIAVVNNARSWRYIETILKSWKENGRNGIY"
     gene            8134..>8586
                     /locus_tag="PKV59_01040"
     CDS             8134..>8586
                     /locus_tag="PKV59_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45365.1"
                     /translation="MESIDDIIERVKKDFNVTPKNPKSLDDMNREAYLGDPNCPICHG
                     IGFVTGDYPIDDPRFGKMDVCSCRKAKLEEETRNQLIRESNLSGYADMSFETFNVEGR
                     GQLRPDERLCLKLAFDHAQKFAKERNGWLLLTGNYGTGKTHLAAAISNA"
BASE COUNT         2396 a         1803 c         2059 g         2328 t
ORIGIN      
        1 cgggttatgg ttgttttgta ggaaatatgg attcaaaatc agcaaattca ccgacacaat
       61 ctctcccggg ttggcattgg gacaggcaat cggccgactg ggaaattatt ttaatcagga
      121 actgtacggt gcgccgagcg atctgccttg ggcgatcacc atcgatccgc aatatcgtat
      181 tcccggattt gaacagcagg caacctatca ccctcttttc ctgtatgaat taatctataa
      241 cttattgaac atgggttttc tgctttgggt cgatcaccga tttggtaaac ggctgaaaga
      301 cggagatatt tttttgattt atttgatcgt ttatccggtc gggcggtttt ttctcgaatt
      361 tttgcggttg gatacggcga tggtgggagg attggacatg aatcaactga tcatgggagt
      421 gaccgctgtt tgtgccacag cggggttgat catccgtcat acttttcagc cgaaaaggga
      481 tttgacaaaa taggcctgat ataggataat aatgcccgtt ctaaagttcc ggacataccc
      541 tcgaagggcg cacggaagag aaaaaatagg cataaaaagt ctacttgacc ggaaccctac
      601 tatgtgttga accttgggag aataaggaaa tggctaatat aaagtcacaa atcaagcgta
      661 acagacagaa cgaaaagaga cgtatccaaa atcgtgttgt tcgcggatct acccgaaccg
      721 ctctatataa ggcacgtctg gctgtcgaaa cgaatcagga aaattccgaa gagatggtga
      781 agaaggcaat aattgctttg gataaagccg ctcaaaaggg tgtaattcat aagaataatg
      841 cagcccgcaa gaagagtcgc ctgataaaga aactgacagc gacaaaaaaa gcgtaacgct
      901 atcgtgaggg agaaatccct ttttgaatgt tattgccgga tcggaaaggg ttgaaagaaa
      961 aagttcaacc cttttttggt catatataga tccgatccgt ccgattctta ttgaaaatgc
     1021 cacatgtgaa gaatgaccag cggaggagcc gtgtttgaaa aagaacaaca actgatagag
     1081 gatcaaattc ggaaaatttg tgaagctcac aattatccgg tgaaatcgct tacctggtcc
     1141 tggattccat tcagcggaaa ttgggggata tccacttctt tttttcaggt cgcggcggat
     1201 gacgcacgtt ccagaggaca aaaaataaat gttggcttgc atgcccaaga aatcgctcaa
     1261 atgacggcgg attcccttga attacccgaa ggattcgaac gtgcggaagc ggttcgagga
     1321 tatttgaatc tctatttcac cactcgaatt tattctcagc gggtcattga tacggtgttg
     1381 gaagaaaaag ataattttgg cagagggcaa cgcaacggtg agcgcgtaat ggttgaattt
     1441 tcccagccga acactcataa agcatttcat gtcggtcatt tacgaaatgt aattctggga
     1501 aatgcggtct gtaatattct cgattttgcc ggtaatgatg tgattcgggc gaattatttg
     1561 ggggatatcg gactgcatgt tatcaaatgg ttgtggtgct atctgaaata tcataacggc
     1621 gagaaacccg agggggatat tacccgctgg atgggtgata tctatgcgga agcaagccgg
     1681 cgccatgaag aagaacccga agcagaaagt gaaatccggg aactctttgc ccgctgggat
     1741 cgaaaagatc cggagattgt cgaattatgg cgtgttaccc gtcaatggag tttggatggt
     1801 ttcgagcaga tttataaaac attgaatgtc cggttcgata agatctattt tgaaagtgat
     1861 gttgaggatt cgggcaaaga aattgtcaaa gaattgattg ccaaaggaat cgccgttgac
     1921 gaacgccccg aaaatgcggt ggtcgtcaaa ttggatgaat tgtgcggtac gaaggataaa
     1981 tatcgttcgt tagtcgtctt gcgctccgac ggcacctctt tgtatgcaac gaaagacctg
     2041 tcgttggcga ttcggaagtt cgaagaattt catctgacgc gttcgatcta tgtcatcgat
     2101 gtcaggcaga cgttatatct gcagcagata ttcaagactt tggaattgat gggttattcg
     2161 tgggcgaaga attgttatca tttggcatat gaaatcgtga atttaccggg aaatgtcacc
     2221 atgtcatcgc gtgacggaac ggttgttttc ctgcaggatt taattgatga ggctaaatcg
     2281 cgagcgttgg cgattgttca agaaaaaaat ccgtcattaa cggatgccga aaaagcggaa
     2341 atatcggaaa aagtagcgct cggtgcgctg aagtattcca tgctctcgcg ggataatgca
     2401 aaaatagtca cttttgactg ggaggcggca cttgatttta acggtcaagc tgctccatat
     2461 atccaatatg ctgcggtacg ggcaaacagt atcctaagaa aatccgaaaa cggattgccg
     2521 gatcaaacag ctttgtctta cgaactgacc ccggtcgaag tggaactgat cgatctgatt
     2581 tcacgggttc cgagggaaat ccaacggtcg gctgcagaat gtaagccgct gtttatttcc
     2641 aacctcgcat ttgatctggc tcaggcattt aataatttct atcatcaatg ccccgtattg
     2701 caggcggagc cgcctgtccg agattaccgg ttacggctgg ctgcggctgc acgccaaaca
     2761 atgatcaatt gcctggctct gttgggaatt gattctccgg aaattatgta attaacaatc
     2821 ataaacaata ataaatcgga agaaataata aaactttcga tttattgttg tttctctatt
     2881 acgttaatac cacccatcca aggtttgagc acttccggga caaccacgga cccgccggct
     2941 tgttggtagg tttccagtac cgcaatcaat gtgcggggca aacccaatcc cgaaccgttc
     3001 aaagtatgta gaaaacgggt ttttccgctt tctttatccc gaaagcggat cgctcctctg
     3061 cgggattgaa agtcaccgtc atcggaaacc gaggaaactt ccagccattc cttacagccg
     3121 ggtgcccaaa cttcgatatc ataacaaatc cgagcattaa atcccaagtc gccggtgcat
     3181 tgggcgacga ttcgataagg caaatccaac tgtctgcagg tttcttcagc ggcatttaac
     3241 atccgatcca gttcatcggc tgatttatcc ggatgactgt aacaatacat ttcaacctta
     3301 tcaaattgat gaccgcgctt aataccgcgc acatcccgtc cggcactcat tttttcgcgt
     3361 cgaaagcagg gagtatatgc ggtaaaacgc atcggaaggc tgtcttcttc gataatttcg
     3421 ttcatataga gaccggtcag aggcacttcg gcggtcggca ccatccaaaa atcttcttcg
     3481 gcatcatgat aaagattgtc tctgaatttc ggcaattggc cggacccgta gagtatttca
     3541 ctttttacca taaacggcgt gtaccgttct tcataaccct gccgggtatg caaatcgagc
     3601 ataaaagcaa tgagtgcgcg ttgcagacgg gcaccggctc cgttcagaat gtaaaaacgg
     3661 ctgccggtca atttcacacc gcgatcaaaa tcgatgatcc ccagcgccgg tcccaaatcc
     3721 cagtgcggaa tcggatcaaa ctctcgttcc gcgattttcc cttcgtagcg gacgaccaca
     3781 ttttccgcat cgctgacacc gtagggtacg cgcggatccg ggatattggg aataatcatg
     3841 atcagcgaat ttaattcctc ttccgtttcc tttattttct tgtccaattc ggcgatttta
     3901 tcgccgacaa ggcgcatcgc ttctattttg gcgtttcttt cctcggtatc cttgatccga
     3961 ccgatctctt tggaagtcgc attccgttcc gctttcagtt tttccgattc ggaaagaagc
     4021 tttcggcggg ccgtatccag ttcgatcaca cgattcaccg gagcgggatc catttgacga
     4081 tctttcatcg atttgatgac caattcggga ttttctcgga tgagattcat atccagcata
     4141 tatagctcct cttatctatc aattaaaaaa tcccccgtcc ggcaggacga aggggtttcc
     4201 gtggtaccac ctgcatttgc tcaaccaaga gcctctttgt cgggagataa cgatcccgaa
     4261 tcggctttct tatcgcatca ttgcttttcg aaagcggtcc gcatttttta tgcgcggggt
     4321 ggataaattc ggacttgttg ccgcctctca tcaaccggcg actctctgtc atccgaaagc
     4381 cgaattaaag tcccgcttga cctatcttat taacgtttat tataaacatt tggagcaaag
     4441 gattcatgaa ccttatgaca ggagatcagg attccttttc acagcagacc ggtgataaag
     4501 cgcactgatc gattttttta tttttcccgt ttccattcgg ttcgagcata tacccctgcc
     4561 ctttgacggt aatcaaatat tgaggattct tcggattttc ctcaatcgcc tttcgcagcc
     4621 attgtatgtg gacatctaac gatcgcatgt cttccaaata gtccgtatcc catagttctc
     4681 ggaataattc ttcccgtttt ataatttcgc cgggacgatc cataaataaa ttgagaagtt
     4741 tggacactcg cggagtgaca ccgactaccc gattatcgat ataaatacaa ttacgggagg
     4801 tatccagtgc caggtcaccc gcgcatttga gtgtgctttt tttcgcggga gcaaaaagca
     4861 taatccgatt caaaagtttt tgaacggtaa acggtaaaca aagaacaaga tcggctttgt
     4921 cggaaatctt cttctgtttg agcatttcat cgatgatgag aatgatcggc aggtccggat
     4981 tgcgcttctt gatacggtag acgatccgat tgcttgctgt tcgcattgaa gccgcatcga
     5041 tcaagacgat catcggatcg gattcttgaa ttttctcaat ggcactatta ccggtggaag
     5101 caatttccac attatacccg cgtttcttta aacttaaaaa gaaagagggg tgatcggtct
     5161 gcttaccttc tatgagaata atcttatcct gaagctgatc caacggcgac tccactgaaa
     5221 taatttactg cccaaaacga gcgatgggaa tgccagtgat tttattgtac atagtattct
     5281 taaaaaagtg tgaaatattt gcgtaaacaa ctttcataaa tatcatatga ctttaatact
     5341 atcaagcatt ctgacaaaga ttatatccaa ggattccgga aatctttcaa aagattaata
     5401 tttctgtgca gtgaagctgc tttataataa tcatatagat tatttcggat tcgaaagctt
     5461 ataatagaaa aactttcaaa atgaagtctg aggatggatg attgaatgtc tgaattgttt
     5521 gaagtaaccg tcgatagtat tcgagtcagt ttaaccacac aacaacgggt attgattttg
     5581 catgaaccga aaaatgaact gtaccttccg atctggatcg gttcgtttga ggcggaatcg
     5641 attgtgatcg cgctgcaggg gattgaagtc agccgccctc aaccgcatga tctactgaaa
     5701 aatgttattg cagcgttgga cgggaaactc aggaacgttg ttctttcgaa gattcaggac
     5761 gatacttttt attcattttt ggttttggaa tcccaaggaa acgaaatact tgtggacagt
     5821 cgcccttccg atgcgatctc tctggcgatt cgttgcaaag tgccgatttt tgtcgataaa
     5881 gccgttttac tttcagccgg aatccaaccc gagaaagata ttcgggaatt aaattcggca
     5941 aagaagaatt tcgccgcgga gccggatcaa gaagagtttg tggatgatgc gaaattgaat
     6001 attttcgatg actttttgaa aaatattgat ctccccgatt ccggtgaaac cggaaagagt
     6061 gaccgttcgg gaaaacagga taacgaaaag aaggacacgg attcaaattc cgatgatctt
     6121 cttttcccga tataagatat ggaaataaac ggaccttatc ctacggagaa tttcactccg
     6181 gaagtttccg aatccgatct acgcttacaa ccgcatagcc gtgaggcgga agaagcggta
     6241 ttgggagcga ttctgattaa ttcggaaaag ttttatgatg tcgctcagat tatctccgcc
     6301 aatgactttt atattcaccg ctgtcgctgg atttgggaag cgtttgccgc tcttctggaa
     6361 aaaggacaag atattgacgt ccaaaccgtc gccaacgaat tggaaaagaa cggaaaatta
     6421 acggaagcgg gcgggaagct ttatctttat tcactggcaa gcaatacacc ctcttcgatg
     6481 cacgccgaat cttatgccaa aatcatcgcg gaaaaaagtg tgcggcgtca gattttgaca
     6541 gcggcaagcc agatgcaaca atatgcctat gacgaatcca aaagtttgga caccattttg
     6601 gataattccg aaaaagcgat tttcaatgtc agcgaaaaaa gaacccgaaa cgaggttcaa
     6661 ccgattcgca gcgtggtggg aacaatttac gatgaagtac ggctcctcgc gcgccaagat
     6721 caggatatcg tcggagttcc gaccggattc aaagatttgg accgcctgtt gggggggatg
     6781 caaaaatccg atttgatcat cgttgccgga cgtcccggaa tgggtaagac cggttttctg
     6841 ctgtccgtat tgaaaaatgc cgcattaaat tatcatatgc acgtcgctat gttttcattg
     6901 gaaatgtcca atgaacaatt agccattcgt ttgatcgcgc aacaaacggg gattaacagt
     6961 caaaagattc gcagcgggaa attggctgaa gatgaacttc cgatgttcat cgatgcattg
     7021 gatgtgttgg ggactacgac cgtttttttg gatgatacgc cggcaattac gccgctgcag
     7081 ctccgatcca aatgcagaag attggatttg gagcatcatt tggatttggt catcattgat
     7141 tatctgcaat taatgagtac cgattcccga aatgagaatc gggttcagga agtttcctat
     7201 atctctcgaa atcttaaaat tttggcgagg gaattaaatg tgccggtttt gacagccgcc
     7261 cagctttcga gaagtgtcga acaacgttcg gaaaaaatcc cgattttgtc ggatctgcgg
     7321 gaatccggtt cgttggaaca agatgccgat gtggtgatgt ttcttcataa ccccaatacc
     7381 gatgacagta aaacagtcgg tttagacgga gcgatgaaat tggttgtcgc caaacatcga
     7441 aacggaccgg tgggagatat cgacctggtc tttgttaaaa agttggctcg ctttgaaaat
     7501 gcagcccgca tttcggacag cggaggaatt cgatgagttt ttccggtttt aatacgggaa
     7561 tcgaggttat cccgactcaa ttctttgagg aaatactgcc tgaaatcgat tcgatgaccg
     7621 aattgaaggt cagtttgttt gcgattcatc ttcttaatca attcgaaggc gaccagcgct
     7681 atatactcta ttcggatttt actgatgccg ccgaatttat ggatggatta gccggaattg
     7741 aagaagatcg cgaagacgct ttgaaggacg gtctcgaaaa ggcagtgtta cgcggcactt
     7801 tgttgaaagt ggaatacggc ggagaatctc tctattttct caacagcccg aagggaagga
     7861 cagcggtgga aaatttagaa aaaggaaaat ggaaaccgga tgcttttctg catctttcgg
     7921 gaacgttgaa tctgtttcgc ccgaacattt tccagttata tgaggaaaac atcggtccgc
     7981 tcactccctt aatggcggat cgtttaaaag acgcggaaca aatgtattcg gaagaatgga
     8041 ttacggaagc catcgatatc gcagtggtga acaatgcccg gagctggcga tatatcgaaa
     8101 cgattttgaa atcatggaag gaaaacggac ggaatggaat ctattgatga cattattgaa
     8161 cgggtaaaaa aagactttaa tgtcaccccg aagaatccga aaagcttgga tgatatgaat
     8221 cgtgaagctt atttgggcga tccgaattgt ccgatttgcc atgggatcgg ttttgtcacc
     8281 ggcgattatc cgattgatga tccccggttc gggaagatgg atgtttgttc ttgccgaaaa
     8341 gccaaactgg aagaagagac ccggaatcaa ttaatccgcg agagtaatct ttccggttat
     8401 gccgacatga gttttgaaac tttcaatgtc gaaggacgcg ggcagctgcg gccggatgaa
     8461 cggttgtgtt taaaattagc attcgatcat gctcaaaaat tcgcgaagga aagaaacggg
     8521 tggctgctgc tgaccggtaa ttacggaacg gggaaaactc atttggcggc agcgatttcc
     8581 aatgcg
//
LOCUS       DAOMIV010000020         8549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8326_length_8549_cov_4.487638, whole genome shotgun sequence.
ACCESSION   DAOMIV010000020 DAOMIV010000000
VERSION     DAOMIV010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8326_length_8549_cov_4.487638"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1855)
                     /locus_tag="PKV59_01045"
     CDS             complement(116..1855)
                     /locus_tag="PKV59_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45366.1"
                     /translation="MKDAAGQLSQKSIQKVMIISAIEGLVALFFTFLHPSENESAVFA
                     GYSAQRFILGGITLVGVALFLLIGKLFSENHPRSEQRVQSLNDFLRQNDRIFFSLVIL
                     SGLTLFAIWCFVFSWLFIPANLRPQIIWVGLIALQSLLCIWRSYGETIRQNHYAEKYR
                     LFPRWKDLSANQKKVFRFLVILSVAYILILIPSNLNGSQNMEAFARYGGDEFVIYPIL
                     MDIMKPGETFSATLYHLFIYEDYHYGYPFYAWSTLVLFPIKLISGENFAEQIQINLPL
                     LRIFVSVIPVILACLVLTFLFTRWKSFWISAPVFLFLLSAPGTLQNNQGFWHPDGLNL
                     LFVCLTLYFLQRDRFRYGRNFYLAAVFTGLSVATRLYGFFFFLAILVYLILGLLRRIN
                     SLGGTIRKGMFFIFVMASVILFADPFLFRADAREKMVTILRQKSTEMAEGYVGDYDPR
                     NDYRTGWDAWYPAFEDHYTEMYCFFFLVFSLVFASFFGDQKLTHLMTLCWCVVIISYL
                     VFFVAVKSTQYVLPALLPLMASIFSIPQAVETGQVPAILRHKSIRIGSWIVAGGILVS
                     QFIINLIKIAPRF"
     gene            complement(1869..3113)
                     /locus_tag="PKV59_01050"
     CDS             complement(1869..3113)
                     /locus_tag="PKV59_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQP45367.1"
                     /translation="MNSYNNNTNFVSSSPDSNRKQPRRSFPWFTAFIMILIGCLIGVL
                     ISSNFVPEPKPETSASAEQIENLQATVTALQNTLKQSQPAANTLNVSYTNIETEITNV
                     VEKVGPAVVTVTANIPTTASYYWFQTSYVATSMGSGVIISDQGYILTNNHVVEGATEL
                     SVELSDGTTLPAKLVNTDEFTDLAVLKVDGTMPGVAKLGESNLLKSGETVIAIGSPLG
                     NFKNTVTVGVVSATGRFLENDKGYKMENLIQTDAAINQGNSGGPLVNLAGEVIGINVM
                     IVRGSSTSSTYAEGLGFAIPSDTVRLIAQQIIEKGKLTRPYLGIQWVDITPRLAENYG
                     LPVKYGVIVLAIEPGSPADQAGLKRNDIITKIGDYEITENSAYYNCLFKYSPGDKVTL
                     EVIRNGKKINIDVKLGGDTTAL"
     gene            complement(3128..3736)
                     /locus_tag="PKV59_01055"
     CDS             complement(3128..3736)
                     /locus_tag="PKV59_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45368.1"
                     /translation="MASSSFLSSVRTTIEPCKKGFKRKFPIWMWQSGLGNVLNAFPSL
                     TGEQVVVILPDNTKLALNCTEKDDVIYALLPAMENPEIYRTLVANPAVQIWSKNGWYN
                     AGARLLTFEETAKTLSSFQPESFFGKIGASLRNPDQDARIRMIEIRRISACTGEKGPG
                     QYAWVWAASSLFFFFSWAHQRKINKRTARKLKVCSSQSNKKK"
     gene            complement(3756..4595)
                     /locus_tag="PKV59_01060"
     CDS             complement(3756..4595)
                     /locus_tag="PKV59_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQP45369.1"
                     /translation="MAKKQEIENNSFTIRDITDVTPAQRIAVLIDGDNAQASLIDKIL
                     AEITKYGLITIRRIYGDWTASNMSGWKDVLQTHAIQPIQQFRYTTGKNATDSAMIIDA
                     MDIFYSGTVDAFCLVSSDSDYTRLATRIREQGLFVMGIGKKTTPRAFVNACDLFVYTE
                     NLVPQNEPLKSTGTTANSAHKKKTAKSAESSPAPDVENGNSPDPLPLLRSAFDLATRD
                     NGWAFLGTLGHHLQQLDPGFDSRTFGYRQLSLLIQAYPDVIETKEVKGADGTSTIYAR
                     LKS"
     gene            4812..6503
                     /locus_tag="PKV59_01065"
     CDS             4812..6503
                     /locus_tag="PKV59_01065"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HQP45370.1"
                     /translation="MKTAGVGEIIPKIDSFYQDCLNLLNEISIESLLNRTAEIAISVT
                     NATYCVCGTTDEEDTFTKIVLRGFTQVELDLIHSDEEAAGQPIRNIHSDAIINIARGK
                     DLPENYNIFPAKRPFISSLLYVPIFYGKEKMGSIILLNKIGAPQFDSIDQKFIEILAR
                     YAGFGINNTIIYERIANREQMLAKRNEDLALLNELAKISASSTENQDAIVHETMHQVM
                     GYLDLVVGEVFLRDDDAPNQFKIVFKEGHNLPTSLFGFSTVQTGQGIVGKVAQSKRNY
                     LLSEEELEIINQKRATTVDLNYVAVLPLITSEGVVGVSCLGTKLVDEKDTLNLQFLSS
                     ISSWMATLIQDYRLTLQRKRVAILEERERISMDLHDGVIQSIYGVGLTLEHARILAKA
                     DPEKVEEQIQASIDALNATIRDIRSYIMDLKPARLTGENLIQSLRRLGNDFYSNTLVT
                     TVFDPKVETLDQISEDYANAFYMICKEALANITKHAKATQVMITFTEEPDRYVLIVQD
                     NGSGFDPGKGRKPESHGINNMFARAENLGGKLEMTSKPGKGTKLEVWLPKTAKEG"
     gene            6508..7272
                     /locus_tag="PKV59_01070"
     CDS             6508..7272
                     /locus_tag="PKV59_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQP45371.1"
                     /translation="MGNDDHTQKISAQAAERGEPSYVWRDGQKRRLKMIQTAAEGRDS
                     GVVLENGCGVGAYLKRLAPVATFSVGLEVEFDRAREALNLVGGERSSVINAVGEELPF
                     PEDTFDLILSHEVLEHVDDDVACLREIVRCLKPGGRLILFCPNRWYPFETHGIYWKGK
                     YHFGNKFGVNYLPRKLRDKLAPHVNVYTTKDLQRKLNGLPVEVISRTVIFGAYDNIIA
                     RSKGLGLMLRKILLFLERTPLKALGLSHFWVIEKKG"
     gene            7436..>8549
                     /locus_tag="PKV59_01075"
     CDS             7436..>8549
                     /locus_tag="PKV59_01075"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HQP45372.1"
                     /translation="MNVADSQRLASALERLGYSESPIIEEADVIVLNTCMVRQSAEDK
                     AIGRLSSLKPLKKKNPDLVLGVMGCMVGFKDQTEIRKKLPYVDVFAPPSNPKPIIDFL
                     IRQEYIGEASASRNVETDTVWEPGKLTLPASRRKKTVAGFIPIVYGCSHACAYCIIPY
                     KRGAERSRNPQEILDHARSLAEQGVKEVTLLGQIVDRYGLDTAGYPTLAELLREIHEI
                     PGIERIRFLTSHPNWITDELLDTVRELPKVMKHIEVPAQAGNNTVLENMRRGYTDEEY
                     RNLISHIREKIPGVSIGTDIIVGFPGETEAQFEDTFRQLADLKLNVVHLARYSPRKGT
                     LSERTMPDDVSDEEKWRRFRAVETLQKQIATELHAAL"
BASE COUNT         2472 a         1789 c         2033 g         2255 t
ORIGIN      
        1 gaaaaatcgg tggaggaaat gcgcaaaagg ccggatctta taaattaacg tggataattc
       61 tcgaaaaatt gtgaacaaaa ctgaaaaaat ctgtggaaat cattgtggat aaactctaaa
      121 aacggggagc tattttgatc agattgatga taaattgcga aaccagaatc ccgcccgcta
      181 ctatccagct gccgattcgg atcgatttgt gccgtaaaat cgcgggcact tgtccggttt
      241 caacagcttg aggaatgctg aaaatagaag ccatcaaagg taacaatgcc ggcaaaacat
      301 attgagtgga tttaaccgcc acaaaaaaga caagataaga aataattaca acgcaccaac
      361 agagcgtcat caaatgagtc aatttttggt ctccgaaaaa ggaagcaaat acaagcgaaa
      421 aaaccaagaa aaagaaacag tacatttccg tataatgatc ttcaaaagcc ggataccagg
      481 catcccatcc cgtccgataa tcgtttcgtg gatcataatc cccgacatat ccctccgcca
      541 tttcggtaga tttttgccgg agaatcgtca ccattttttc ccgtgcatca gcccgaaaca
      601 ggaacggatc cgcaaataaa attacggatg ccatgacgaa aataaaaaac atccctttac
      661 gaattgtccc gccgaggctg tttatccttc gcagtaatcc gaggataaga taaaccagaa
      721 tcgccaggaa aaagaaaaaa ccatagagtc gggttgccac ggaaaggccg gtgaaaaccg
      781 ccgccaaata gaaattgcga ccgtatcgaa atcgatctcg ctgcagaaaa taaagtgtca
      841 gacaaacaaa cagcaaattc aatccatcgg gatgccaaaa tccctgattg ttttgcagcg
      901 tccccggtgc cgataaaaga aagagaaaaa cgggagcgct gatccaaaaa cttttccagc
      961 gggtaaataa aaaggtgagg accaggcagg ctaaaatgac cgggatcacc gaaacaaaaa
     1021 ttcgaagtaa tggcaaatta atctgaatct gttccgcaaa attttctccc gaaatcaact
     1081 ttatcggaaa cagaaccaac gttgaccaag cataaaaagg gtagccgtaa tgataatctt
     1141 cataaataaa caaatggtaa agtgtggcgg aaaatgtctc cccgggtttc atgatatcca
     1201 tcaagatcgg gtaaataaca aactcatcac cgccataccg ggcaaacgct tccatgttct
     1261 gcgatccgtt aagatttgac ggtatcaaaa tcaggatata agctacggat agaatgacaa
     1321 gaaagcgaaa aactttcttt tgatttgcgg acagatcttt ccaacgcggg aataatcgat
     1381 atttttcggc ataatgattt tgacgaatgg tctccccgta acttcgccat atacaaagca
     1441 agctttgcaa agcaattaag cccacccaaa taatttgcgg acgcaaatta gcggggataa
     1501 aaagccatga aaatacaaaa caccaaatag caaaaagggt caaacccgaa agaataacga
     1561 gagaaaagaa aatccgatca ttttgcctga gaaaatcgtt cagtgattgt accctctgtt
     1621 ctgatcgggg gtggttctct gagaacaatt ttccgatcaa caagaaaagc gccacgccga
     1681 ccagcgttat tccgcccaag atgaatcttt gcgcggaata accggcaaaa acagccgatt
     1741 cattttcaga cggatgcaga aaagtgaaga aaagcgcgac caatccctcg atcgcgctta
     1801 taatcattac cttttgaatc gatttttggc taagctgacc agctgcatct ttcatattcg
     1861 gatcattttt acaatgcggt tgtatctcct ccgagtttca catcgatatt gatcttcttc
     1921 ccgtttcgaa tcacttcgag ggtaacttta tctcccggag agtatttaaa gagacagtta
     1981 taatatgcgc tgttttccgt gatttcataa tctccgattt ttgtaatgat atcattccgc
     2041 tttagtccgg cttgatccgc gggactgccc ggttcgatgg caagaacgat gacgccgtac
     2101 ttaacaggca atccgtaatt ttccgccaaa cgcggagtga tatccaccca ctgaattccc
     2161 agatagggtc tggtaagttt tcccttttca ataatctgtt gcgcaattaa cctgactgta
     2221 tcggacggga tggcaaaacc taatccttcc gcataagtgg acgaggtact ggaaccgcga
     2281 acgatcatga cgttgattcc gataacttca ccggctaaat tgactaacgg tccgccggaa
     2341 tttccttgat tgatcgctgc atcggtctga atcagatttt ccatcttata ccctttatcg
     2401 ttttcaagaa aacgaccggt ggcactgacc acgccgacgg taacggtatt cttaaaattc
     2461 cccagcggtg agccgatcgc gataaccgtt tctccggatt tcagcagatt ggattcgccc
     2521 aattttgcca cacccggcat ggttccatcc actttcaaaa ctgccaagtc cgtaaattca
     2581 tcggtattga cgagttttgc cggaagtgta gttccgtctg acagttcgac gctcagttcc
     2641 gttgcccctt ccacaacatg attgttggta aggatatacc cttgatcact gataatcaca
     2701 ccgctgccca tactggtcgc aacatagctt gtctgaaacc agtaatagct ggctgtagtc
     2761 gggatgttag cggtaaccgt tacgacggcg ggtcctactt tttcaacaac gttggtaatt
     2821 tctgtttcga tattggtgta actgacattg agtgtatttg cggccggttg agattgcttc
     2881 aaagtattct gcaaagcagt caccgtcgct tgaagattct cgatctgttc ggcggaagcg
     2941 gatgtttccg gcttcggttc cggaacaaaa tttgaagaga tgagaacccc aatgaggcat
     3001 ccgatgagga tcatgatgaa cgcagtaaac caggggaatg accgtctggg ctgtttccga
     3061 ttggaatcgg gagacgatga aacgaagttt gtgttattgt tataagaatt cacagttcac
     3121 cttcttatca ctttttctta ttcgattgac tcgaacagac ctttaattta cgggctgtcc
     3181 gtttgtttat ttttctttga tgcgcccaag aaaagaagaa gaacagggat gaagcagccc
     3241 atacccaggc atattgtccc ggtccttttt cacccgtaca ggccgatatt ctccggattt
     3301 cgatcatacg gattcgagca tcttgatccg gattgcgtaa agatgcgccg attttcccga
     3361 agaatgattc cggttgaaag gaagataaag ttttcgcggt ttcttcgaaa gtcaacagac
     3421 gggcaccggc gttataccag ccgttttttg accagatttg gacggcggga ttggcaacca
     3481 aagtccggta gatctccgga ttctccatag ccggcagcaa ggcataaatg acatcgtctt
     3541 tttcggtaca atttaaagcc aatttggtgt tgtccggaag gataacaacc acttgttccc
     3601 cggttaagga aggaaatgcg ttcagaacat tccccaaacc ggattgccac atccagatcg
     3661 gaaattttct cttaaaacct tttttgcacg gttcgattgt cgtccgaacg gaagataaaa
     3721 aagaagagga tgccatttcg atattctctt tccggttatg acttcaaacg agcataaatc
     3781 gtcgaagttc cgtcggctcc cttgacttcc ttggtttcga tcacatccgg atacgcctga
     3841 atcaacaaag ataactgtcg ataaccgaaa gtacgggaat caaaacccgg gtccaattgt
     3901 tgtaagtgat gacccagagt gccgagaaaa gcccatccgt tatcacgggt cgcaaggtca
     3961 aaagcactcc gtaacaacgg cagcgggtcg ggtgaattgc cgttttcgac atccggagcc
     4021 ggactggatt cggcggattt ggcggttttc ttcttatgcg ctgaattggc ggttgttccg
     4081 gtgcttttta atggttcatt ctgcgggacc aagttctcgg tatagacaaa cagatcacag
     4141 gcgttgacaa aggcacgggg tgtggtcttc ttaccgatcc ccataacaaa aagtccctgt
     4201 tcgcgaatgc gggttgccaa tcgtgtataa tcactgtcgc tcgaaaccag gcaaaacgca
     4261 tcgaccgttc ccgaataaaa gatatccatc gcatcgatga tcatcgcgct atccgtcgca
     4321 tttttaccgg ttgtgtagcg aaattgctga ataggttgaa tggcatgagt ctgcaaaaca
     4381 tccttccacc cgctcatatt ggatgctgtc cagtccccat agattcgtcg aatggtgatt
     4441 aatccgtatt tggttatttc cgcaagaatt ttgtcaatca aagaggcttg tgcattatca
     4501 ccgtcaatca ataccgcaat tctctgagcg ggtgtaacgt cagtgatatc ccggattgtg
     4561 aaactattat tttctatttc ctgctttttc gccacttatt tcttccttta ttctcgatcc
     4621 gttattaaga tcggaaatgc ttctttttca taattatagt agagaatccg ttactttaat
     4681 attaaagttg aataataaaa agagaaaatc aatgaaatga acaaatcgga ttgataaacc
     4741 gtacagtata aggaggattt acgacaattt gagtcgggtt ttagataggt tcaaggtaaa
     4801 ataaattgaa tatgaagaca gccggtgtag gggaaataat cccgaaaatt gattcgtttt
     4861 atcaggattg cctgaatttg ctcaacgaaa tttcgattga atcgttgttg aatcgaacgg
     4921 cggagattgc aatttcagtc acaaatgcga cctattgtgt ctgtggtaca acggatgagg
     4981 aagatacctt tacaaaaatc gtcttacgcg gttttactca ggttgaattg gatttgattc
     5041 acagcgatga ggaagctgcc ggtcagccga ttcgcaatat ccattcggat gcaatcatta
     5101 atatcgccag aggaaaagac cttcccgaaa attacaatat tttccccgcg aaaagacctt
     5161 ttatcagctc cttactatat gtccctattt tttatggcaa ggagaaaatg gggtcgatta
     5221 ttttgctaaa taagattggt gcccctcaat ttgattcgat cgatcaaaaa tttatcgaga
     5281 ttttggcgcg atatgccggg tttgggatca ataatacgat aatttatgag cgaattgcca
     5341 atcgggagca gatgcttgcg aagcgaaatg aggatctggc tttactgaat gaactggcaa
     5401 agatttccgc ttcgtccaca gaaaaccagg atgcgatcgt tcacgagacg atgcatcagg
     5461 tgatgggata tctggatttg gttgtcggag aagttttttt gcgggatgat gacgctccca
     5521 atcagttcaa aattgtattc aaagaagggc acaatctgcc taccagtttg tttgggtttt
     5581 cgactgttca gacgggacag ggaattgtcg gaaaagtggc acaatctaag cggaattatt
     5641 tgctttcgga ggaagaactg gaaattatca accagaaaag agcgacaaca gttgatttaa
     5701 actatgttgc ggtccttcct cttattacaa gcgaaggggt tgtgggagtc tcttgtctcg
     5761 gtacaaaatt ggtggatgaa aaagatacgt taaatcttca gtttttgtct tcgatcagca
     5821 gttggatggc aactttgatt caagactatc gtttaacgtt gcaaagaaaa cgggttgcca
     5881 ttctggaaga acgggaacgc atcagcatgg atttacatga cggtgtcatt caatctattt
     5941 acggtgtggg gttgacgctg gagcatgccc gcattttggc aaaagcggat ccggaaaaag
     6001 tcgaggaaca aattcaggca tccatcgatg cattaaacgc gacgatccgg gatatccggt
     6061 cctatattat ggatcttaaa ccggcacgct tgaccggtga aaatttgatt caaagtttgc
     6121 gccggttggg gaatgatttt tattcgaata ccttggtgac gactgttttt gatcccaagg
     6181 ttgagacatt ggatcaaatt tccgaggatt atgcgaatgc tttctatatg atctgcaaag
     6241 aagcgttggc gaatattacc aagcatgcca aagcgaccca ggtgatgata acttttacgg
     6301 aagaaccgga tcgatatgtt ttgattgttc aggacaacgg gagtggattt gatccgggga
     6361 aagggcgaaa accggagagc catggtataa acaatatgtt tgcccgggcg gagaatctcg
     6421 gcggaaaact ggaaatgaca tccaaacccg gcaagggtac gaaactggaa gtttggcttc
     6481 cgaaaacggc gaaagaagga taaattcttg gggaacgacg atcatacaca gaaaatttcc
     6541 gcacaggcag ccgaacgggg agagccatct tacgtttgga gggacggcca aaaacgcaga
     6601 ttgaaaatga tccaaacagc tgccgaaggt cgagacagcg gggttgttct ggaaaacggt
     6661 tgcggtgtcg gggcttatct aaaacgactg gctcccgtcg caactttcag cgtcggcttg
     6721 gaagtggaat tcgatcgggc acgggaggct ttaaacctgg tcggtggcga acgatcatct
     6781 gtcataaatg ccgtaggaga agagttgccg tttccggaag atacttttga tctgatattg
     6841 agtcatgaag tattggaaca tgtggatgat gatgttgcct gcctgcgaga aatagttcgt
     6901 tgcctgaagc cgggaggaag gttgattttg ttttgcccca atcgttggta tccttttgaa
     6961 actcatggaa tttattggaa agggaaatac catttcggaa ataagtttgg ggtcaattac
     7021 ttaccgcgaa aattaagaga taaactggca ccacatgtga acgtatatac gaccaaggat
     7081 ttgcagcgca aattgaatgg tcttccggtc gaagtcattt ccaggacggt cattttcggc
     7141 gcttatgata atattattgc ccgttcaaag ggattgggac tgatgctgcg aaaaatcttg
     7201 ctttttttag aaagaactcc cttgaaagcg ctgggccttt cccatttttg ggtgattgag
     7261 aaaaaaggtt gacagcttgt ttatagttta cgaagataaa aatcggaacg aatcgaaaga
     7321 ttaaaccata ttcggatgag aattcttggg aatggctgtt ccttttccgg atcgtttgaa
     7381 tgtatgaaag tatgattgaa tgatgaaata ttatatttgg actgaaggtt gccagatgaa
     7441 tgtggctgac tctcagcgat tggcatccgc attggaacgt ttggggtata gcgaatcacc
     7501 gatcatcgaa gaagcggatg tgattgtcct gaatacctgc atggttcggc aaagtgccga
     7561 agataaagcg atcggcaggc tcagctcgct gaaaccactc aaaaagaaaa atccggatct
     7621 ggtgttgggc gtaatgggtt gtatggtcgg atttaaagat cagacggaga ttcgcaaaaa
     7681 attgccttat gtcgatgttt ttgcgccgcc ctccaatccg aagccgatta tcgatttttt
     7741 gattcggcag gagtatatcg gtgaagcatc agcttcccga aatgttgaaa cggatacggt
     7801 ttgggaaccg ggcaaattga ctttgccggc tagccgccga aaaaaaacgg tcgccggctt
     7861 tattccgatt gtttacggtt gttctcatgc ctgtgcttac tgtatcattc catataagcg
     7921 tggagcggaa agaagccgca atccacaaga aatacttgac cacgcgcgtt cgttggctga
     7981 gcaaggtgtg aaagaagtca ccttattggg gcaaatcgta gaccgttacg gattggatac
     8041 agccggttat ccgaccttgg ctgaactgtt gcgggaaata catgaaatcc ccggcatcga
     8101 acgaattcgg tttttgacca gccacccgaa ttggataacg gatgaactct tggatacggt
     8161 gcgcgagtta ccaaaggtca tgaaacatat tgaagttccg gcacaagcgg gcaataatac
     8221 ggtgcttgaa aatatgcgtc gcggttatac ggatgaagag tatcgtaatt tgatttctca
     8281 tatccgcgaa aagataccgg gggtctcgat cgggacggat attattgtcg ggtttcccgg
     8341 agaaacagaa gcgcaatttg aagatacatt ccgacaactt gcggatctga aactgaatgt
     8401 ggtgcatctt gcccgttatt ctccgcgaaa gggcacttta tcggagcgaa cgatgccgga
     8461 tgatgtctcc gatgaagaaa aatggcggcg ttttcgggcc gttgagacgt tgcagaaaca
     8521 gattgcgacc gagcttcacg cggccttgg
//
LOCUS       DAOMIV010000021         8482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8410_length_8482_cov_4.544796, whole genome shotgun sequence.
ACCESSION   DAOMIV010000021 DAOMIV010000000
VERSION     DAOMIV010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8410_length_8482_cov_4.544796"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKV59_01080"
     CDS             <1..352
                     /locus_tag="PKV59_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45373.1"
                     /translation="KQIWLVILVLVVVSMLIDLNTRISTLTYLREQKNTLAVEVTALQ
                     STLDLVSEKIDYAGSDAAVESWARQQGLMRQEGDYVIIPLPVDEPTPIPTPVPTVEPV
                     KYENWEIWKALIFD"
     gene            399..1277
                     /locus_tag="PKV59_01085"
     CDS             399..1277
                     /locus_tag="PKV59_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HQP45374.1"
                     /translation="MTAVIIDGKKIAEGIRNEVGIAVKERIAAGLSVPGLATVLVGND
                     PASQIYVRNKMKACDRLGIYSVSYTLPEDTDFETLKELINQLNVAPEIHGILVQLPLP
                     PQIDEYSVLSMINPQKDVDGFHPINAGMLARKESEPWFIPCTSAGILRLIHSVQPDIT
                     GMEAVVIGRSSIVGMPTAQLLCRNNATVTICHTKTVNLAAVCRRADLLVVAAGHSKLV
                     KGDWVKPGAIVIDVGINRVNTPDSPKGYILTGDTEFDEIVNIAKAITPVPGGVGPMTI
                     AMLMQNTLLAASRYSA"
     gene            1429..2817
                     /locus_tag="PKV59_01090"
     CDS             1429..2817
                     /locus_tag="PKV59_01090"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HQP45375.1"
                     /translation="MSTLLIKNALVLATMDDNRQEISGGGLLIRDGFIESVGKTSDLP
                     AEADEILDLSGCVVLPGLINTHHHFYQTLTRAIPGAQNSNLFNWLKTLYPIWGRMTPE
                     DIYISTQTAMAELALSGCTTASDHLYLFPNGSKLDDEIEGARQVGLRFHASRGSMSLG
                     ESKGGLPPDSVVDSEEHILEDCVRVVEKYHDPKPGSMLQIVFAPCSPFSVSTELMRES
                     AKLARHYGVHLHTHLAETEDEEAFTLEKFGLRPVDYMASVDWLGNDVWFAHSVHVSPE
                     DIQVYKKYGCGVAHCPSSNMRLGSGFAPLMEYLREGIHVGLGVDGSASNDGSHMLAEV
                     RQALLMARLRAGLMGASLSGENAPKLITAREVLEIATRGGAAVLGRKDIGSLEPGKCG
                     DFIAVNMNRLRYTGSLTDPLSALVFCNPGWVDFNYVQGKAIVKNGELMTVNVPDLVQR
                     HNKAAAGLVRNY"
     gene            2831..3571
                     /locus_tag="PKV59_01095"
     CDS             2831..3571
                     /locus_tag="PKV59_01095"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQP45376.1"
                     /translation="MKNQENNYENLSLFQKLNNDLAKIIEETPPGTRLKSEPELAASL
                     GVSRATLREAMRTFEGQGLIRRRQGVGTFVLDQKQNIESGLEVLESIETQAKRLGLDV
                     KMGYLEINHIKTDEELAKHFGIQQGDPVIRISRVIWVADRPVAYLIDNVLPDVLEEED
                     LDKGFTGSVLDLFLRRGKPELSHSKTLVAAFPATTAIAKKMQLQRGDCLLRLEAYLYD
                     KKDRMIDYSLSYFIPGFFKFQVIRSIGK"
     gene            3576..5612
                     /locus_tag="PKV59_01100"
     CDS             3576..5612
                     /locus_tag="PKV59_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45377.1"
                     /translation="MTNKSSSGSNRPSLPVLFTLAILIAYGVPVFLGWFSFYGDDWIY
                     IYEYHLNGTAGFPAFVAWDRPYSAWIYILATSLFGEHVFPYHLLLLLQRWLAVFLFFK
                     ILQMVWSDIPRVVSGAVLLFAVYPGFHQQPIAVQYILHFASLDLCLFSVWCMLKAFDS
                     GFLEKRSWKGILLTIAGFFSSLTALFSCEYFIGLELVRPFLLWFRQVNRFKDEPVKKP
                     VKIGNIFFHWFPYLLSMMIFIVWRVFIFSFQTYQPKLLNKFNENPAAALSEILRKILT
                     DLYTVTIAAWKKSLNQPAGKENQVIFWLLILVCFFLFAYSLRRFHNREGNKQPAAIEN
                     EKIQFLITGLVALLAAGIPYWVTMINIELDFPWDRPTISFSIGVAMLISVGISFIFQN
                     KFQTLVTASLIAFAIGSHYTNALVYRNEAEKMNDYFWQLAWRAPQLQPGTILVSEQIP
                     LDRYSDNDLTPIVNWQYAPALKGNTYQYKYFDLDLRSETYFRDLRPGQPIKHDYRSHH
                     FESDSSHVLALFYKKGSCLWTITESEAEYPGLPENIVHVAGISDINLIRSDSTVQAVP
                     PSPIGKEPDHGYCYYFQEINRAFQNHDRETAYQAADTALKMGLSSQDPLDFIPAVKAL
                     ILGEDWSRAAELSKKVIMNPENLPFLCEQISGIPETVNQPEYTEMLKTIGCVTQ"
     gene            5648..6904
                     /gene="thrC"
                     /locus_tag="PKV59_01105"
     CDS             5648..6904
                     /gene="thrC"
                     /locus_tag="PKV59_01105"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQP45378.1"
                     /translation="MNKFTGYRCSLCGKEFDASYSGYVCDADQGNLNVILNYDQIRNS
                     IDPKQIFQSAEPSLWRYAPLLPVEDPGFFGTPLHSAGWTPVYDEPRLAAKIGVRNLWI
                     KDDGRNPTASFKDRASAVLIARAKEINAKTIVTASTGNAGAALAGMAAASGWHAIIFA
                     PKSAPPAKIAQLLVFGAEVILVDGNYDSAFDLSVSAAERYGWYCRNTGYNAFTAEGKK
                     TAAFEIWEQIISKQNTSSAPLNVFVSVGDGNIISGIHKGFSDLKELGWLPQMPRIFGV
                     QSEKSSAVYQAWAKGSEEIIPVHATTFADSISVDLPRDGVRAVRAATKTGGAFIAVSD
                     ESIYRGISELGKIGVFAEPAGAAAYVGMEKAAEEGLIGPDNDVVVINTGSGLKDIRSA
                     MKATGEAVIIEPSLAALEKYLKAKGN"
     gene            6929..7903
                     /locus_tag="PKV59_01110"
     CDS             6929..7903
                     /locus_tag="PKV59_01110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQP45379.1"
                     /translation="MKPTYSVIVPVYNEKESLPVLYERMVSVMDQTGEPWELILVDDG
                     SFDGSSQIMRELSQKDPRVRVIFFARNFGHQIAVTAGMDHALGDAAIVIDADLQDPPE
                     VILELIEKWKEGYEVVYAVRTGREGESWFKKETASVFYRLIYKITDVKIPLDAGDFRL
                     LDRKVLDVMNSMREKHRFLRGMSAWVGYKQIGVPYQRAPRYAGKTKYPLKKMILLAWN
                     AITGFSYVPLQIAMILGFIVAFISILLIIVVIILRINGNELVGQATTLISTLFLGGVQ
                     LICLGIIGEYLGRIYDEVKNRPLYIAAEITPSIYDEDSEKPSEQERKE"
     gene            8028..>8482
                     /locus_tag="PKV59_01115"
     CDS             8028..>8482
                     /locus_tag="PKV59_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HQP45380.1"
                     /translation="MARIDLTIKKDRLEHAVERARQRNIIIPTFEEQIHPELVPEKIK
                     KELKSIGLWDIHPRNLYRITWKNEPVEKGGTFGDVNYIELPSSLTGVDARIVALVGKW
                     FPTGAHKVGAAFACLVPRLVTGQFDPTEEKAVWPSTGNYCRGGAYDSALL"
BASE COUNT         2288 a         1862 c         2062 g         2270 t
ORIGIN      
        1 aaagcaaatc tggttggtta ttttggtttt ggtcgtcgtc agcatgttga tcgatctgaa
       61 tacccgtatc agcacattga cttatttgcg agaacaaaaa aataccttgg cagtggaagt
      121 aaccgcttta caatccacgt tggatctcgt atcggaaaaa atcgattatg ccggttccga
      181 cgctgcggtt gaatcttggg caagacaaca ggggttgatg agacaggaag gggattacgt
      241 cattattccg cttccggtgg atgaacccac accgattccc acaccggttc cgaccgtgga
      301 accggtcaaa tatgaaaatt gggaaatctg gaaagctttg atttttgatt aaataaaaaa
      361 ggaacagatg agcccgatcc atacggctcg gctaaactat gaccgctgtc atcattgatg
      421 gaaagaaaat agcagaagga atccgaaacg aggtcggcat tgccgtaaaa gagagaatcg
      481 ctgccggttt gtcggtcccg gggttagcga ccgtgttggt tggcaatgat ccggcttccc
      541 aaatctatgt ccgaaataaa atgaaggctt gtgaccggtt gggaatttat tcggtttctt
      601 atacgcttcc ggaagatacc gattttgaaa cgctcaaaga gttgatcaat caattaaatg
      661 tcgcgcccga aattcatggc atccttgtcc aactcccatt accgccgcaa atcgatgagt
      721 attctgtttt atcgatgatc aatccccaaa aagatgtgga cggatttcat ccgattaacg
      781 ccggtatgct ggcccgaaaa gaaagtgagc cttggtttat cccatgcact tcggcgggta
      841 ttctgcgttt aattcacagt gttcaaccgg acatcaccgg tatggaagcc gtagtaatcg
      901 gccgatcttc tatcgtcggt atgccgaccg cgcagttgct gtgccgaaat aacgccacgg
      961 tcaccatctg tcacacaaag accgtcaatc tcgctgccgt ttgccgccgt gcggatctgt
     1021 tggtagtcgc tgccggacac agcaaactgg taaaagggga ttgggtaaaa ccgggcgcga
     1081 ttgtgattga tgtgggaatc aatcgagtga atacgcccga ctcacccaaa ggatatatcc
     1141 tgacaggtga taccgaattt gatgagattg tcaacattgc caaagcgatc acgccggttc
     1201 caggcggagt cggtccgatg acgatagcaa tgcttatgca aaataccctg ctcgccgctt
     1261 ccagatattc cgcttaacat tttctttctt caaatttcat ttcgatttgt cgtatattat
     1321 gatgaatatc atattgtcag acaatccaaa atggggtata aacaagggag tagaactgct
     1381 ctaaatgatt agacaattct ttgcttatca aaaaggagga aagaatcatt gtccacactt
     1441 ttaattaaaa atgcattggt actggctacc atggatgaca atagacagga aatctccggc
     1501 gggggattat tgattcggga cggatttatc gaaagcgtcg ggaaaacatc ggatttgccg
     1561 gcggaagcgg atgaaattct ggatttatcc ggctgtgtcg ttcttccggg gctgattaat
     1621 acacatcatc acttttatca gacgttgacc agggcaattc ccggcgctca gaattcgaat
     1681 ctatttaatt ggttaaaaac cctttatccg atttggggca gaatgactcc ggaagatatc
     1741 tatatttcga cacagacagc catggcagaa ttggcgcttt caggctgtac aaccgcttcc
     1801 gatcatttat accttttccc gaacgggtct aaattggatg atgagatcga aggtgctcgc
     1861 caagtgggat tgcgtttcca tgcatcgcgg ggatcgatga gcctcggcga atccaaaggc
     1921 ggacttccgc cggacagcgt cgttgacagc gaagaacata ttttggaaga ttgtgttcgg
     1981 gttgtcgaaa aatatcatga tccgaaaccg ggatcgatgc tgcaaattgt ttttgcacct
     2041 tgttctcctt tcagtgtctc aaccgaatta atgcgggaaa gcgcaaaatt agcccgtcac
     2101 tacggagtcc atttgcacac ccatttggcg gagacagagg atgaggaagc ttttacactc
     2161 gaaaaattcg gcctcagacc ggtagattat atggcttcgg tcgattggct cggaaatgac
     2221 gtctggtttg cacattccgt ccatgtcagt ccggaagata tacaagttta taagaaatac
     2281 ggttgcggag tcgctcactg tccgtcatcg aatatgcgtc tcggctccgg tttcgctccg
     2341 ttgatggaat atttacggga aggaattcac gtcgggttgg gagtggacgg gtccgccagt
     2401 aatgacggct cccatatgtt ggcggaagtt cgacaggctc tcctgatggc acgccttcgt
     2461 gccggtttga tgggcgcttc gctcagcggt gaaaatgcgc ccaaattaat caccgctcgg
     2521 gaagtgctgg aaattgcgac gcgcggcgga gcggcggttc tgggcagaaa agatatcggc
     2581 tctctggaac cgggaaaatg cggcgatttt atcgccgtca atatgaatcg attacgctat
     2641 accggcagct tgaccgatcc gctgtccgct ttggtattct gcaacccggg ctgggttgat
     2701 ttcaattatg tccaaggaaa agcaatcgtt aaaaacgggg aattaatgac cgtgaatgtg
     2761 ccggatctgg tacaacgaca taataaagcc gctgccggat tggtgagaaa ttattgaatc
     2821 aggtgattag atgaaaaacc aagaaaacaa ttatgagaat ctcagccttt ttcagaaact
     2881 gaataatgac ttagcaaaaa ttattgaaga gacacctccc ggtaccagac tgaaatccga
     2941 gccggaattg gcggcaagtt taggcgtgtc tcgcgctacg ttgcgcgaag cgatgcgaac
     3001 gtttgaaggt caggggctaa tccgtcgccg acagggagtc ggaacttttg tcctcgatca
     3061 aaagcaaaac atcgaaagcg ggctggaagt gcttgaaagc atcgaaacac aggcaaaacg
     3121 gctggggttg gatgtgaaga tgggatatct ggaaatcaac cacatcaaaa ccgatgaaga
     3181 actcgccaaa cacttcggca tccaacaggg tgatcccgtt atccgaattt caagggtaat
     3241 ctgggtcgct gaccgtccgg tggcatattt aatcgataat gtattgccgg atgttttgga
     3301 agaggaagat cttgataaag ggttcaccgg ctcggtgttg gatttattcc tgagacgagg
     3361 gaagccggaa ctctcacact ccaaaacatt ggttgccgct ttccccgcca caaccgctat
     3421 cgcgaaaaaa atgcagctcc aaagaggaga ttgtctcctc aggttggaag cttatcttta
     3481 tgacaaaaaa gatcgaatga tcgattattc tctcagctat ttcattccgg gatttttcaa
     3541 attccaagtg attcgatcaa tcgggaaata aatttatgac caataagtca tcctccggaa
     3601 gcaaccggcc ttctctaccg gtcctgttta ccttggcgat tctgatcgct tatggggttc
     3661 cggtattttt aggctggttc agtttctatg gggacgactg gatttatatc tatgaatatc
     3721 atctgaacgg taccgccgga tttcccgctt ttgtcgcttg ggaccggcca tattccgcat
     3781 ggatctatat tttggcaact tctcttttcg gagaacatgt tttcccgtat caccttttat
     3841 tgcttcttca gcgctggttg gctgtctttt tgtttttcaa aatcttgcaa atggtttggt
     3901 cggatattcc gcgtgttgtc agcggagccg tattactctt tgccgtatat cccggctttc
     3961 accaacaacc gatcgcagtc caatatattt tgcatttcgc atcattggat ttatgcttgt
     4021 tttccgtttg gtgcatgctg aaagcttttg attccggttt tttggagaaa cgatcatgga
     4081 aaggtatcct gctcacaatc gccggattct tttcttcgtt aacggcactt ttttcctgtg
     4141 aatattttat cgggctggaa ttggttcggc cgttcctgtt atggttccga caagtcaatc
     4201 gatttaaaga cgaaccggtt aaaaagccgg tgaaaatcgg aaatatcttt tttcactggt
     4261 tcccatatct gttatccatg atgattttta tcgtttggcg ggtttttatt ttctcattcc
     4321 agacctatca gccgaagctt cttaacaaat tcaacgaaaa ccccgctgcc gcattatcgg
     4381 agattctccg aaaaattctg accgatcttt atacggttac gatagcggca tggaaaaaat
     4441 ccttaaacca acctgccgga aaagagaatc aggtcatctt ctggttgttg atattagtct
     4501 gttttttcct ctttgcttat tctctcagac ggtttcacaa ccgagagggg aataaacaac
     4561 cggcggcgat cgaaaatgag aagattcaat ttttgatcac cggactggtc gctttgttgg
     4621 cagccggtat tccgtattgg gtgacaatga tcaatatcga actcgatttc ccttgggacc
     4681 ggccgaccat ttctttcagt atcggtgtcg ctatgttgat cagcgtcgga atttcgttta
     4741 ttttccaaaa caaatttcaa accttggtca ccgcgtcgct gatcgcattc gctatcggct
     4801 cccattacac caacgccctg gtctatcgta acgaagccga aaagatgaac gattattttt
     4861 ggcaacttgc ttggcgagcg ccgcaattac aaccggggac aattttggtt tccgaacaga
     4921 ttccgctcga tcgctacagt gataatgatc tgacaccgat tgtcaattgg caatatgcac
     4981 ctgctttaaa agggaatact tatcaatata aatattttga tttggatttg cgcagcgaga
     5041 cttattttcg ggatctccga cccggtcagc cgattaagca tgattatcgc agtcatcact
     5101 ttgaatcgga cagcagtcac gttctggcat tattttacaa aaaaggttcc tgcctttgga
     5161 cgattaccga atcggaagcc gaatatcccg ggttaccgga aaatattgtt catgtggccg
     5221 gaatttccga tatcaatttg attcggtccg attcgaccgt tcaagccgtg ccgccgtctc
     5281 cgatcggaaa agaaccggat cacggatatt gctattattt tcaggaaatc aaccgcgctt
     5341 ttcagaatca tgacagagaa acggcttatc aagccgcgga taccgcgtta aaaatgggat
     5401 tatcctcgca ggatcctttg gattttatcc cggcggtgaa agcactaatc ttaggcgaag
     5461 attggtcccg agccgcggag ttaagtaaaa aagtcatcat gaacccggag aatctgcctt
     5521 ttttgtgcga gcaaatatcc gggattcccg aaacagtcaa tcaaccggaa tatactgaaa
     5581 tgctgaaaac aataggttgt gtaactcaat aaaaaatcaa accgacaagg aaacaggacg
     5641 aagaatcatg aataaattta caggatatcg atgcagtctc tgcggaaagg aatttgatgc
     5701 ctcttattcg ggttatgtct gtgatgcgga tcagggaaat ttgaacgtga tccttaatta
     5761 cgatcaaatc cgaaactcga tcgatccgaa acaaatcttt caatccgccg aaccttctct
     5821 gtggcgttac gcaccgttgt tgcccgtcga ggatcccgga ttttttggga ccccgcttca
     5881 ttctgccgga tggactccgg tttatgatga gccccggttg gcagcaaaaa tcggcgtccg
     5941 taacctttgg atcaaagatg atggacgaaa tccgaccgct tcttttaaag atcgagccag
     6001 cgccgttctg attgcccgag cgaaagaaat aaatgccaaa accattgtca ccgcttccac
     6061 gggaaatgcg ggagcggctt tggcagggat ggctgctgcc agcggatggc atgccattat
     6121 ttttgccccc aaatcggcac cgccggcaaa aatcgcacag cttctggttt tcggcgccga
     6181 ggtgattttg gtagatggaa attatgacag cgcttttgac ctttccgtat ccgccgcaga
     6241 acgatacggt tggtattgcc gtaacacagg ttacaatgct tttaccgcag aaggcaaaaa
     6301 aaccgccgct tttgaaattt gggaacaaat tatttctaaa caaaacacat catccgcccc
     6361 attaaatgtc tttgtttccg tcggagacgg aaatattatt tccgggattc ataaaggatt
     6421 cagcgatctt aaagagctgg ggtggcttcc tcagatgcca cgcattttcg gggtccagtc
     6481 agagaaatca tccgccgttt atcaggcatg ggcaaaaggc agcgaagaga ttatcccggt
     6541 tcatgcgacg acatttgccg attccatctc cgtggatctg cctcgggacg gcgtgcgcgc
     6601 ggttcgagcg gcaacgaaaa ccggcggagc tttcattgcg gtttcggatg aaagtattta
     6661 ccgtgggatc tccgaactcg gtaaaatcgg ggttttcgcc gaaccggccg gggctgcagc
     6721 ttatgtcgga atggaaaaag cagcggaaga aggattgatc ggaccggata acgatgtggt
     6781 ggtaataaat accggaagcg gattgaaaga cattcgctcc gccatgaaag caaccggaga
     6841 agcggtgatt atcgagccca gtcttgccgc tttggaaaaa tatctgaaag cgaaagggaa
     6901 ttagcccttc caaaaaaagg attgcaatat gaaacctact tattctgtga tcgtcccggt
     6961 ttacaacgaa aaggaaagcc tcccggtttt atatgaacgg atggtttccg taatggatca
     7021 aaccggcgaa ccttgggaat tgatactggt ggatgacgga tcctttgacg gatcatccca
     7081 aatcatgcgg gaactttctc aaaaagaccc tcgtgtccgg gttatctttt ttgcccgcaa
     7141 tttcggccat cagattgcgg tgactgccgg tatggatcac gctttgggcg acgcggctat
     7201 agtaatcgat gccgatcttc aggatccgcc tgaggtaatc ttggaattaa tcgaaaaatg
     7261 gaaggaaggg tacgaagttg tttatgccgt aagaacagga agagaggggg aatcgtggtt
     7321 taagaaagag acagcttccg ttttctatcg cttaatttat aaaataaccg atgtgaaaat
     7381 tccgttggac gccggcgatt tccgtctgtt agatcgcaaa gtcttggacg tgatgaattc
     7441 catgcgagag aaacatcgat tcctgcgagg aatgtcggca tgggtaggtt ataagcaaat
     7501 cggtgttccg tatcaacgag ctccacgcta tgcaggaaag accaaatatc cgttgaaaaa
     7561 aatgatcctg ctggcttgga acgcaattac gggtttttct tacgtcccgc tgcaaattgc
     7621 gatgattctc ggcttcatcg ttgcattcat cagtatctta ctgatcatcg ttgttattat
     7681 tctgcgcatc aacggcaatg aattagtcgg acaggcaacg acattaatct caactttatt
     7741 ccttggcggc gttcagttga tttgtttggg aatcattggc gaatatttgg gacggatcta
     7801 tgatgaagta aaaaatcgac ctctctatat tgctgcagaa attactccct ccatctatga
     7861 cgaagactcg gaaaagccat ccgaacagga aagaaaagaa taaatttacg ggaggaatca
     7921 tttattcgat acagaatatc agaatgtcaa aaagattata taagttttag ttatctaaaa
     7981 cgagacctgc aaacaaaaca aactattaag aaataagagg gaaaaaaatg gcacgaattg
     8041 atttaacaat caaaaaagat cgcttggagc acgctgttga acgcgctcgg caaagaaata
     8101 ttatcatccc aacctttgaa gaacagattc atccggaatt agttccggag aaaatcaaaa
     8161 aggagttaaa gtctatcggt ttatgggata ttcatccgcg aaacctttat cgaatcacct
     8221 ggaaaaatga accggttgaa aaaggcggaa ccttcggtga tgtcaattat atagaattgc
     8281 cttccagttt gaccggcgtc gatgccagaa ttgtagcttt ggtggggaaa tggttcccga
     8341 ccggtgccca taaagtcggt gcggcgtttg cctgtctggt tcccagactg gtaactggac
     8401 aatttgaccc gacggaggag aaagcggttt ggccttccac aggaaactat tgtcgcggcg
     8461 gagcttatga ttctgcatta ct
//
LOCUS       DAOMIV010000022         8440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8459_length_8440_cov_4.242338, whole genome shotgun sequence.
ACCESSION   DAOMIV010000022 DAOMIV010000000
VERSION     DAOMIV010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8459_length_8440_cov_4.242338"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKV59_01120"
     CDS             <1..887
                     /locus_tag="PKV59_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQP45381.1"
                     /translation="QMKKNLDILMVGHFAKDKIVIDGNEFESPGGAVYYGSVVLRRLG
                     LEVGVATRGNPDDFHYLKELTDLGIEVDAKPAPQSSGCINIYQSADMERRICKLNGFA
                     GEIPYEDIPDVNAKIIMVTPIIAGEITLPTLIKLSEKAPIALDVQGFVRVPEGNDLNF
                     RPWKDMEEGLKHVTFLKVDKAEAEHITGTDDLEKAAVILAGYGPKEIVLTQSKEVLVY
                     ADGKFYRAPFTPKSLAGRTGRGDTCITSYVGSRLKYDPERSCKIAGIITTMKQEKHGP
                     WTGEIDPQRFDPSIVFGD"
     gene            937..1521
                     /locus_tag="PKV59_01125"
     CDS             937..1521
                     /locus_tag="PKV59_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45382.1"
                     /translation="MAEKKVSRKKVNMNAASSNEEENLESGTKEQSLNTAETNNSLPQ
                     AEENEIKGASESQSNIIESPANIHQDFFEENKKVEEKKMEENEAAAGSVNPVPEFEKL
                     PDPFIQEPEISDKKIPAENEGIEKDASEEGKTVEIPETSPSGELPKDVFEEGTGEEKK
                     NNNNIWLWIVIAIIVIIFLCFCGFIGSIVFLFWG"
     gene            1616..1975
                     /locus_tag="PKV59_01130"
     CDS             1616..1975
                     /locus_tag="PKV59_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45383.1"
                     /translation="MKENNPIVYSTEKGRICPECGFPEKECHCRTKKISKIISDDGTI
                     RIRKEKKGRNGKEVTIIQGISLSEQAVQSIVSDIKKKLGTGGTVKDDIIEIQGDRVKF
                     IAEYFIKLGYKVQINGG"
     gene            1965..3662
                     /gene="ettA"
                     /locus_tag="PKV59_01135"
     CDS             1965..3662
                     /gene="ettA"
                     /locus_tag="PKV59_01135"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQP45384.1"
                     /translation="MGGKMAVEQNKIIYSMIGVSKYYDKKVILKDIYLSYFYGAKIGV
                     LGLNGAGKSSLLKILAGVDKDFIGETVVAPGYTIGYLEQEPLVDSKKTVREVVEEGAQ
                     EAINLLKEFEEISNKFSEPMSPDEMDRLIEKQGKIQEKIDALDAWDIDSRLEMAMDAL
                     RCPPGDSPVDILSGGEKRRVALCRLLLQKPDILLLDEPTNHLDAEAVAWLERHLQQYK
                     GTVIAVTHDRYFLDHVAGWILELDRGQGIPYQGNYSSWLEQKKIRLEQEEKGESQRQR
                     TLARELEWIHMSPKGQHAKSKARISSYEKLLTQDFTKANEELQLYIPPGPRLGNLVVE
                     MKDVQKVFGNRIIMDHVTFTLPPGGVIGIIGPNGAGKTTLFRMIVGEEKPDSGTIRIG
                     ETVKLGYMNQSRDSLDPEKTIWEVISEGQDEIKFGDRKMNSRAYVARFNFSGADQQKK
                     VGVLSGGERNRVQLALTLKSEANVLLLDEPTNDLDVNTIRALEEGLENFGGCALVISH
                     DRWFLDRIATHILAFEDDSSVIFFDGNYTQYEADRRNRLGIEADTPHRIKYRSLTRI"
     gene            3920..4939
                     /gene="iolG"
                     /locus_tag="PKV59_01140"
     CDS             3920..4939
                     /gene="iolG"
                     /locus_tag="PKV59_01140"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HQP45385.1"
                     /translation="MNRIRVGIIGAGRIGRVHAQSIAFGIPEAEIVAISDPFIENAER
                     AARDFAIPAIYADYHAILNDPTIQAVLICSPTNTHADIAMEAARAGKHIFCEKPVDQT
                     VSKILETKKLVEENAVKMQIGFNRRFDHNFRKIKKLRDDGTLGEIQIIRITSRDPEPP
                     SPEYAKTSGGMFLDMTIHDFDMACYQANSEVDEVYAMGAVTVDPEIGKAGDIDTAIVT
                     LKFKNGAMAVIDNCRKAVYGYDQRVEVFGTNGAAASENDRETMVKISSKDAVCEDKPL
                     FFFLERYMQSFRDEIKSFINAILNDRPVEVNIDDGLRPILIAIAANKSMKENRPVKIS
                     EVMEG"
     gene            4944..5756
                     /locus_tag="PKV59_01145"
     CDS             4944..5756
                     /locus_tag="PKV59_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HQP45386.1"
                     /translation="MSKKIIKGKPFDFGYNPITEIGGDPDMLMDFGILRLKSGQIYED
                     NSALERAYLLVYGEITLEYNKSSIKINRPNCFDYAPWVLHLPANVPVRITGVAEDSEI
                     TVNKTVNSRIFDPKLYEPKDSQDEFRGAGTMQETSTRIVRTTFDYNNAPYANLVIGEV
                     IGFPGKWSSYPPHFHPQPEIYYYKTYPTGGHAFAEVGDDVFKISGNDTVLIQNGMTHP
                     HVTYPGYALWYLWAIRHLDGNPYITPTFVPEHEWVAKPDAKFWSYSNKKEMK"
     gene            5753..7621
                     /gene="iolD"
                     /locus_tag="PKV59_01150"
     CDS             5753..7621
                     /gene="iolD"
                     /locus_tag="PKV59_01150"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HQP45387.1"
                     /translation="MIKRIRLTMAQALIKFLDNQYVEFDGEEDRFVDGMIGVFGHGCV
                     TGVGQALEQGGHSLRFYQGHNEQGMAHVAIGYAKQKNRRKILPCLSSIGPGALNMVTA
                     CGTATANRIPLLVLPGDTFACRQPDPVLQQVEFPDSYSTTVNDSFRAVCRYWDRISRP
                     EQLMTAMIHAMRVLTDPADTGAVCVALPQDVEAEAYDYPEYFFRKRVWHMDRRPITDR
                     QLEQAIDLISNSKKPLLICGGGVRYSEAWKDLQKFAEEFNIPIGETQAGKGLIAWDHP
                     LNLGGIGVTGTKAANLIARDADLIIGVGTRFTDFTTNSKWGFRNPECKILAINVCSFD
                     AVKMDAEGILADAREALSAIRKELKNLNYQSKYTDEIIEAKKEWDDIVNEYYNLPEEK
                     NGIPQTRALGEINRFMRSKDIAVGSAGSLPGDMQRLFRPGKPGTYHMEYGFSCMGYEI
                     NAAVGVKMAAPDSEVYAFLGDGSFIMLHSELFMAIQEGLKINIMVFDNSGWGCIESLQ
                     NNQGTKTFGTVFRARNPETDLLDGEILPINFAKIAEGYGAKGYTIHNADELKKALKNS
                     KMQEKPCVFDIKVLPGSMTPGFESWWRVGVAEVAEDQSVNEAYAEMQKHIAEARDY"
     gene            7633..>8440
                     /gene="iolE"
                     /locus_tag="PKV59_01155"
     CDS             7633..>8440
                     /gene="iolE"
                     /locus_tag="PKV59_01155"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HQP45388.1"
                     /translation="MLNPEKVKLGIAPIGWTNDDMPDLGAENTFEQCISEMALAGFKG
                     CEIGNKFPSDPAVLKHKLEVRGLQICNAWFSSYLIGRPYEETIASFIRHRDKLNYLGA
                     KIIGASEQGNSIQGKIDVPILENKPIYTEEEWKIVTKGFNEMGELAREKGMFFTVHHH
                     MGTGIQTEEEIDRIMEMTDPNLVYLLFDSGHLSFAGIDPVHILKKYIHRVKHVHLKDL
                     RYNVYAEVKAKRMSFLDAVRAGVFTVPGDGDVDFKPIFNILEEAEYEGWIV"
BASE COUNT         2647 a         1646 c         1952 g         2195 t
ORIGIN      
        1 atcaaatgaa aaaaaattta gacatcctaa tggtcggtca tttcgcaaaa gataaaatcg
       61 tcatcgacgg aaatgaattc gaaagccccg gcggagccgt ctactacggc agcgtcgtct
      121 tacgcagact cggtttagaa gtcggtgtcg caacacgagg aaacccggat gatttccact
      181 atctgaaaga actgaccgat ctcggaatcg aagttgacgc gaaaccagca ccacaatcct
      241 cgggatgcat caacatctat caatccgccg acatggaacg aaggatttgc aaactgaacg
      301 gattcgcagg tgaaattcct tacgaagata ttccggatgt aaatgcaaaa atcattatgg
      361 ttacaccgat tatcgccgga gaaattacgc tccctacctt aatcaaactt tccgaaaaag
      421 cacccatcgc attagatgtt cagggctttg tcagagttcc cgaagggaat gatctcaact
      481 tcagaccttg gaaagatatg gaagaaggac tgaaacatgt cacatttttg aaagtcgata
      541 aagcggaagc cgagcatatt accggaaccg atgatcttga aaaagccgct gttatcttag
      601 ccggatatgg tccgaaagaa attgttctga cccaatcaaa agaagtcctg gtatatgcgg
      661 acggaaaatt ttatcgtgca ccttttactc cgaaatctct cgccggcaga acaggcaggg
      721 gcgatacctg tattacttct tatgtcggtt caaggctaaa gtatgatccc gaacgatcct
      781 gtaaaattgc cggaattatc acaaccatga aacaggaaaa acacggccct tggaccggcg
      841 agattgatcc tcaacgattc gatccttcaa tcgttttcgg tgattaatag ttttttgttt
      901 ttagattatt attaatgtat aaacaaggag gggaaaatgg cagaaaagaa agtttctaga
      961 aaaaaagtaa atatgaatgc ggcttcttca aacgaagaag agaatcttga aagcggtact
     1021 aaagaacaat cgctaaatac cgccgaaaca aacaattccc ttcctcaagc cgaagaaaat
     1081 gaaataaaag gcgcttcgga aagtcaaagc aatattatcg aatcaccggc caatattcat
     1141 caagattttt tcgaagaaaa caaaaaagtt gaagaaaaga agatggaaga aaacgaagca
     1201 gccgcaggtt cagtcaatcc tgttcccgaa tttgaaaaac tccctgatcc tttcattcaa
     1261 gaaccggaga tttcagacaa gaagattccc gccgaaaacg aaggaatcga aaaggatgca
     1321 tccgaagaag gaaaaacggt tgaaattcct gaaacttctc cttccggtga actgccgaaa
     1381 gatgtgtttg aggaaggaac cggtgaggag aagaagaata ataataatat ctggttatgg
     1441 attgtcattg ctatcattgt tattattttc ttgtgttttt gcggttttat cggctccatc
     1501 gtctttctgt tttggggcta accggcgttt atcgaatact attgttccat atcgattgag
     1561 acaggatctt ttatagaaca aaaagatcct gtttgtttgc tataattatg ctcctatgaa
     1621 agaaaacaat ccgatcgtct attctaccga aaaaggacgc atctgtcctg aatgcggttt
     1681 tccggaaaaa gaatgccact gccggacaaa aaagatctcg aaaattattt ccgatgatgg
     1741 cacgattaga attcggaaag aaaagaaggg aagaaacgga aaagaagtca ccataattca
     1801 agggatctct ctttccgaac aagccgttca atcgattgtt tccgatatca aaaagaaatt
     1861 gggaaccgga ggaacggtta aagacgacat cattgaaatc caaggggatc gggtgaagtt
     1921 tatagcggaa tatttcatta agctcggata taaagtccaa ataaatgggg ggtaagatgg
     1981 cggttgaaca aaacaaaatc atctattcga tgatcggggt ttcaaaatat tatgataaaa
     2041 aggttattct caaagatatt tacctctcct atttttacgg tgcaaagata ggtgtcctcg
     2101 gattaaacgg tgccggaaaa agttcgttat taaaaattct tgccggagtt gataaagatt
     2161 tcatcggtga aaccgttgta gcacccggtt ataccatcgg ttatcttgaa caggaaccgt
     2221 tggttgacag taaaaaaaca gttcgtgaag tggttgaaga aggtgctcaa gaagctatca
     2281 acctattaaa agaattcgaa gagatcagta acaaattttc cgagcccatg agtcccgatg
     2341 aaatggatcg attaatagaa aaacaaggca aaattcaaga gaaaattgat gcactggatg
     2401 cttgggatat cgattcccgc ttggaaatgg caatggatgc cttgcgttgt ccgcccgggg
     2461 attctccggt cgacatttta tccggagggg aaaaaagaag ggttgccttg tgtcgtttat
     2521 tgctccaaaa acccgatatc cttcttctag atgaacccac caaccacctt gatgccgaag
     2581 ctgtagcttg gttggaaagg cacttacaac aatacaaagg aacggttatc gccgtcacgc
     2641 acgatcgtta ttttttagat catgtcgccg gctggattct tgagttagac cgcgggcagg
     2701 gaattcctta tcagggaaat tattcatctt ggctggaaca gaaaaaaatt cgtttggaac
     2761 aggaagaaaa aggagaaagt caaaggcaaa gaacgcttgc tcgagaactg gaatggatcc
     2821 atatgtctcc caaaggacag cacgccaaat ccaaagcgag aatttcttcc tatgaaaagc
     2881 ttttaaccca ggattttaca aaagccaatg aggaattaca gctgtatatt cctccgggtc
     2941 cgcgtttggg gaatttggtg gttgaaatga aggatgtcca aaaagtcttc ggaaaccgaa
     3001 ttattatgga tcacgtgacc tttacgctcc cacctggcgg agtcatcggt attatcggac
     3061 cgaacggtgc cggtaaaacc actttatttc gcatgatcgt cggagaagaa aaaccggatt
     3121 ccggtaccat tcgaatcggt gagactgtta aattgggata tatgaatcag agtcgggact
     3181 ctttggatcc ggaaaagaca atatgggaag ttatttcgga agggcaggat gaaataaagt
     3241 tcggtgatcg aaaaatgaat tccagagctt atgtcgcccg ttttaatttt tccggagccg
     3301 atcagcaaaa aaaagtcgga gtcctgtccg gaggggaaag aaatcgcgtt caacttgcat
     3361 taactttaaa aagcgaggca aatgttcttc tcttggacga gccgacaaat gacctggatg
     3421 tcaatacgat tcgtgcactt gaagaagggc ttgaaaattt cggaggttgt gctcttgtca
     3481 tctctcatga tcgttggttt ctcgatcgca tcgcaactca tattctcgct ttcgaagatg
     3541 attcttctgt catctttttt gacggtaact atacacaata tgaagcggat cgacgaaatc
     3601 gtctcggcat tgaagccgat acacctcacc ggatcaaata tcgatctttg acccgaatat
     3661 aagaaaaaat cagcaaccgt tattgatatc ttgctgatcg atcagaagga aaataactct
     3721 ttccgctgtt gttgatgtcg attcaattca tttactgaca atatgaatta tttcatttaa
     3781 atcaattaca tttctcgtaa ttcgattcat tatttccatg ttatgataaa agaaggtttt
     3841 gtaaacgttt acaatgccaa ctgacagata acagatagat aaacttttta ttttcactat
     3901 ccctaaggag tttgttccta tgaatagaat tcgagtcgga attattggag caggccggat
     3961 cggacgagtc catgctcaat caattgcatt tggaatcccc gaggcagaaa tcgtcgcgat
     4021 ttcagatccc tttattgaaa atgcggaaag agccgcaaga gatttcgcta ttcccgccat
     4081 atatgcggat tatcatgcca ttttgaatga tcctactatt caagcggttt taatttgttc
     4141 tccgaccaat acccatgcgg acattgcaat ggaagcagcc cgagccggta aacacatttt
     4201 ttgtgaaaaa ccggtagatc aaactgtttc aaaaatactt gaaacaaaaa aattagtcga
     4261 agagaacgcc gtaaaaatgc aaatcggctt taatcgccgc ttcgatcata atttcagaaa
     4321 gattaaaaaa ctcagagatg acggaacatt gggagaaatt caaattatcc gaataacttc
     4381 cagagatccc gaaccgcctt caccggaata tgccaaaact tccggcggaa tgttcttgga
     4441 tatgaccatt catgattttg atatggcatg ttaccaagca aattctgaag ttgatgaggt
     4501 ttatgcaatg ggtgctgtta cggtcgatcc tgaaatcgga aaagcgggcg atatcgatac
     4561 ggcaatcgtg acactcaagt ttaaaaacgg cgccatggct gttattgaca attgcagaaa
     4621 agctgtttat ggttatgatc aacgggtcga agttttcgga acaaacggag ccgcggcatc
     4681 cgaaaacgat cgggaaacaa tggttaaaat tagttcaaaa gatgcggttt gcgaagataa
     4741 gcccttattc ttctttttgg aaagatatat gcaatctttc agagatgaaa taaaatcttt
     4801 tatcaatgcg attttgaacg atcgacccgt tgaagttaat attgatgacg gattacgtcc
     4861 gatcctaatc gcaattgccg caaataaatc gatgaaagaa aatcgacctg taaaaatatc
     4921 ggaggttatg gaaggataat attatgagta aaaagattat caaaggaaaa ccattcgatt
     4981 tcggctataa tccgattaca gaaatcggtg gcgatccgga tatgttaatg gattttggca
     5041 ttttaaggct aaaatccgga cagatttatg aagacaactc tgctttggaa agagcttatt
     5101 tgttggttta cggagaaata acgctcgaat ataataaatc atccataaaa attaatcgac
     5161 cgaattgttt tgattatgct ccttgggtat tgcacttgcc ggctaatgtt ccggtaagga
     5221 taacgggagt ggcggaagat tcagaaatca ctgtcaataa aactgttaat tcgcgaattt
     5281 ttgatccaaa gctatacgaa cctaaggatt ctcaggatga attcagagga gccggaacga
     5341 tgcaggaaac atccacgcgc attgttcgaa ctacatttga ttacaacaac gccccctatg
     5401 cgaatttggt aatcggagaa gtgatcggtt tccccggcaa atggagttcc tatccgcctc
     5461 attttcatcc gcagccggaa atttattatt acaaaaccta tcccaccggc ggccatgcat
     5521 tcgccgaagt cggtgatgat gttttcaaaa taagcggaaa tgatacagtc ctgattcaaa
     5581 acggaatgac tcatccgcat gttacttacc ccggatatgc cctttggtat ttatgggcga
     5641 tacgtcattt ggacggaaat ccgtatatta caccgacctt tgtaccggaa catgaatggg
     5701 ttgccaaacc ggatgcaaaa ttttggtctt acagcaataa aaaggagatg aaatgatcaa
     5761 gagaatccga ctgacaatgg ctcaggcgct tattaaattt ctggataatc aatatgtcga
     5821 atttgacggt gaggaagacc ggttcgttga cggaatgatc ggagtctttg gtcacggttg
     5881 cgtcaccggc gtcgggcagg cattggaaca aggcggacac agtctgcgat tttatcaggg
     5941 gcataacgaa cagggtatgg cacatgttgc aatcggatat gccaaacaga aaaacagacg
     6001 gaaaatcctt ccctgcctgt cgtccatcgg ccccggcgct ttgaatatgg ttaccgcatg
     6061 cggtaccgcg acagcaaatc gaatcccttt actcgtttta ccgggagata ctttcgcttg
     6121 ccggcagccg gatccggttc tgcaacaagt cgaatttccg gatagttata gtacgacagt
     6181 gaacgattct ttccgagcgg tttgtcgcta ttgggatcga atcagtcgac ccgaacaatt
     6241 aatgacagct atgatccatg cgatgagagt actgacagac cccgcggata ccggtgctgt
     6301 ttgcgtggct ttaccgcaag atgtcgaagc cgaagcttac gattatccgg agtatttttt
     6361 ccgtaaacga gtttggcata tggatcgccg tcccataaca gatcgacaat tggaacaggc
     6421 aatcgacttg atttcaaaca gcaaaaagcc tctgctgatt tgcggtggag gtgtgcgcta
     6481 tagtgaagcg tggaaggatt tacaaaaatt tgccgaagaa tttaatatcc ctatcggtga
     6541 aactcaggca ggaaaaggtt tgattgcttg ggaccaccct ctgaatctgg gcgggatcgg
     6601 agttacaggc accaaagccg ctaatttaat tgcgagagac gccgacttga ttatcggtgt
     6661 cggaacacgt ttcaccgact ttacgaccaa ttcaaaatgg ggattcagaa atcccgaatg
     6721 caagattctg gcgattaatg tgtgctcttt tgatgccgtc aagatggacg cggaagggat
     6781 attggcggat gcccgtgaag ctctctcggc gattcgaaaa gagttgaaga atctcaatta
     6841 ccaaagtaaa tatacggacg aaattatcga agccaagaaa gaatgggatg atatcgtcaa
     6901 tgaatattac aaccttccgg aagaaaaaaa cggtattccg caaaccagag cattgggaga
     6961 aatcaatcgc ttcatgcgct caaaagatat cgctgtagga tctgccggaa gtttgcccgg
     7021 agatatgcag aggctgttcc gcccgggaaa acccggtact tatcacatgg aatatggttt
     7081 ttcctgcatg ggatatgaaa taaacgcagc cgtcggggta aaaatggcgg ctccggatag
     7141 cgaagtgtat gctttcttgg gtgacggcag ttttattatg cttcacagcg aactttttat
     7201 ggcaatccaa gaaggattga aaatcaacat aatggttttt gataactcag gttggggttg
     7261 catcgagagt ctccaaaata atcagggaac aaaaacattt gggactgtct tccgggcacg
     7321 taatcccgag acagatttgt tggacggaga aattcttccg attaacttcg caaagatcgc
     7381 tgaaggatac ggggccaaag gatatacgat tcataatgcg gatgagctga aaaaagcatt
     7441 gaagaattct aaaatgcaag aaaaaccctg cgttttcgat attaaagttc ttcccggcag
     7501 catgactccg ggatttgaaa gttggtggag agtgggtgtg gcggaagtag ccgaggatca
     7561 atctgtaaac gaagcgtacg cggaaatgca gaaacatatt gccgaagctc gagattatta
     7621 ggaaggggag aaatgcttaa tccggagaaa gttaaacttg gaattgctcc gatcggatgg
     7681 acaaacgatg atatgcccga tttgggagcg gaaaatactt ttgagcagtg tatcagtgaa
     7741 atggcgttgg ctgggttcaa aggctgtgaa atagggaata aatttccctc tgatcccgct
     7801 gtcttgaaac acaaattgga agtccgtggt ttacaaatct gtaacgcatg gttttcaagc
     7861 tatcttatcg gcagacccta cgaagaaacg atcgcttctt ttatccgcca tcgtgacaaa
     7921 ctgaattatt tgggagcgaa gatcatcggt gcatcggaac agggaaattc gatccaaggg
     7981 aaaattgatg tccctatact tgagaataaa ccgatttata ccgaagaaga atggaaaatc
     8041 gtcacaaaag ggtttaacga aatgggagaa cttgcccgcg aaaaggggat gtttttcacc
     8101 gtacatcacc atatgggaac cggcattcaa acagaagaag agatcgatcg aatcatggaa
     8161 atgacggatc cgaatttggt atatctttta tttgactccg gacacctgag ttttgcgggt
     8221 atcgaccctg ttcatatatt gaaaaaatat attcatcgtg tgaaacatgt ccatctgaaa
     8281 gatctgcgat acaacgtcta tgccgaagtc aaagcaaaaa gaatgagctt cttggatgcc
     8341 gttagagccg gtgtttttac cgttccagga gatggtgacg ttgattttaa accgattttt
     8401 aatatccttg aagaagcaga gtatgaaggt tggatcgtag
//
LOCUS       DAOMIV010000023         8422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8480_length_8422_cov_5.243695, whole genome shotgun sequence.
ACCESSION   DAOMIV010000023 DAOMIV010000000
VERSION     DAOMIV010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8480_length_8422_cov_5.243695"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(513..1796)
                     /locus_tag="PKV59_01160"
     CDS             complement(513..1796)
                     /locus_tag="PKV59_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQP45389.1"
                     /translation="MKIAIIGSGLTGMTAAWNLRKQGHEVTIFEKEKEPGGLARGFRE
                     PQWQDSVECFYHHIFTGDSDILSLFKDLGVSNSVEFRSPKTVMYYRGKFYPFDSIPSA
                     ILYPGLGLGSKIRFGFVGLYLRLFSPWKEMEKVTAEEWMLKYAGEHVYRSMWEPMMLG
                     KFGEKYAKEVNMAWLWARIHSRSTSLGTFRGGFQTFINIFSEKLIANGVEIRFESNVT
                     QIKRQKESSITVTADGKNDDFDRVIATISPAAFSAISPDLPADYRQSLLDLKNMGAVV
                     MVLSLKHPLSPEGYYWYNLPKSEGFPFLSLVEHTNFVPRERFGNETIIYAGDYLETDH
                     EYFSLPKDELLQRVLPGLKKINPNFEEDWVINSWKFSAKYAQPIPFINQSEHIPSVET
                     PINGLYLASMSQIYPWDRGMNYAVRMGNTVARMIG"
     gene            1934..2923
                     /locus_tag="PKV59_01165"
     CDS             1934..2923
                     /locus_tag="PKV59_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQP45390.1"
                     /translation="MPEQSFPFIFIHLNQSCDFISLFNSLVKLGGIPVAFLSAEPVRT
                     ENEFIRSVPVVPFSGNNFQEALGNLLFHSEKNTGLESFFVICSDDAVISGSELNEQLA
                     SARKNPQAFYLLEILTTQESVEDNGIVRMIRWIRRKLEGPKASLWIIPPQAITSANPY
                     SEDKKFSLFPWLLKCERQGIPIFLTQVNRKNDGAVAVNSLRRINLLFYTLNTFLRYSG
                     SSVLSFLVDNTVFFLGIQIGFGNLLALVTGRVASLLVNYFLLKSVVFKPKNRKNRSFA
                     KYVGLVIFSGSVVWSLITIGEKIIPIHPIFLKLAAELGMFFFNYYVSKTFIFN"
     gene            3021..3917
                     /locus_tag="PKV59_01170"
     CDS             3021..3917
                     /locus_tag="PKV59_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HQP45391.1"
                     /translation="MKRKKYADELTEEELRFRLMQKKTIGRDARIASYRAGGRVMDAA
                     NAPINSELKWNSDRSEKEVLKTAPPQKKFDKILLAIELLAVAGVIVMLVIGSQILQNI
                     NRESAKSMILPTLTPTPLIQAVVLPGGHTPPDESGVGTFNESEIPEHLRGIVSVMAPQ
                     PTIPAGMEQVIRLRIPAINVDAPVVLGDDWEALKAGIGMNLYSAAPGKNGNIILSGHN
                     DIYGQVFRDLDQLMPGDQIIVLTEKNAYTYSVQNIEVVLPNQVEVMAQTTDPTVTLIS
                     CYPYLVDDKRIVVTGKMLYIEE"
     gene            3936..4145
                     /locus_tag="PKV59_01175"
     CDS             3936..4145
                     /locus_tag="PKV59_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45392.1"
                     /translation="MAKKNTTKGKAPIKTTATAVSSSGNLPLAARAPKEEFNPDYTYV
                     IRNLKKIGTLAAVFIAILIILSFII"
     gene            4239..5345
                     /locus_tag="PKV59_01180"
     CDS             4239..5345
                     /locus_tag="PKV59_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45393.1"
                     /translation="MPINLNPVPARIDPKVLAALAKPQSGNPFSLSEKIEDRFREMFF
                     PDAASINFFNCSREGLYEVALNSFASGPVLIGMNGPFSQQWQTIAKSLNIDSEIIDTA
                     FGSGFDEDLFEKALDEESYAAVFLVEIDPFTGTQIDIAALSGMIRKKLPDALVIVDCS
                     ASFGSVRTLDLKSEADALLIGSEISLGLPPGLGTVILGERANFKSIGFSGTGWSLNYA
                     RQFRDIHSKSFRSLIPFQLLNALDRQLDLIFLEGIENRIKRIVSLSEKMKGWADANRF
                     EIFTRPQFRSPTVTVLKKPLPFTVQNLTDFLSSYGIIIGNCPDDMRDDYFVIAHMNQT
                     TEEEMTLFLDVLDRFLADYDTKKSIPVVSKLSPQ"
     gene            complement(5359..6855)
                     /gene="crtI"
                     /locus_tag="PKV59_01185"
     CDS             complement(5359..6855)
                     /gene="crtI"
                     /locus_tag="PKV59_01185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HQP45394.1"
                     /translation="MKPLNVIIIGAGLGGLACGIRLAVEGCKVKIFEKNHFAGGSLGS
                     IRLKGFTFNTGAEYISAPFLFDQLYEYAGKRRIDSFKFRDCDPIFQAVFPDFKRFALY
                     SNPAETIKLNPMLSGADLQGFNSFTEESAAIFDDIFFDYCGRPVDENTFRFRTNLWLR
                     RMNPQKTCEEYANEKFQTPELQQLFGFWPLIGGGNPKSATHLFRAFSQMFQRWGTAVP
                     DGGMDNMVQAIVRLFRESGGEIFYGTEVSEIQIFNHTVSGVRLKDGRIQQADLVISDA
                     DAAATYRYLIDSDRTRYDSVNQAKTKISGISAFVYHLGTRCILQERAPLAPLNILFPE
                     DYDRFLNELFDQKTLSDHPLLILRMASKIIPDNAPFGCEALSVIVPVPNLESINPWDH
                     ISYSYRNHVLDLLQNYFLTDIRTNLIVEEYLDPIIIKNRLGSHAGSILSFQPELQKNG
                     EIRMANRCRDIRHLYLAGNGAHPGATIPGVLLGAANTVQVIKEDFDKE"
     gene            complement(6866..7951)
                     /locus_tag="PKV59_01190"
     CDS             complement(6866..7951)
                     /locus_tag="PKV59_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQP45395.1"
                     /translation="MKNYKIPMVPGPVRVTPVAYRSLTSDFGAGYCEDEFFELYQTVG
                     KKLGILLGTQSEVIIQSGEAMSILWGAIKSCVLPGEKVLAISTGFFGEGFADMARAIH
                     ADAQLVEFGYDETVHDLDRIESAIKTYQPKVITIVHCETPSGTLNPISEVGQLKKKYN
                     VPIMIVDAVASVACTEIEGDKWNADIVMGGSQKGLSLPPTIGFCSVSEQAWDIIRKVD
                     YAGYEAFWSYHNIPQKHDHPNTPDWPGIAALNDVLDDLMINEGLSNVFARHQVVAEFV
                     RKSLTEIGYRLFPKPEAIQSPSVTAVYVPADRTFEAFDQQVRTYGMGIGGSYGKIAGK
                     VFRLGHMGTQATMTNARNAIDVLQQVI"
     gene            complement(7948..>8422)
                     /locus_tag="PKV59_01195"
     CDS             complement(7948..>8422)
                     /locus_tag="PKV59_01195"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQP45396.1"
                     /translation="AYTLWVDGEPIDSADINNPLVYLHGHDNIIPGLENELNGLFPGE
                     KKTVIVSPEDGYGELMPDSIVELDRSLFPEDYIPALGSVLHLQDESDQVYTAYIQDFN
                     ENSVIVDLNHPLAGKELKFDIEIVEVRDASEGELKAGHIHHDCSSCSGCEDGSCE"
BASE COUNT         2393 a         1807 c         1766 g         2456 t
ORIGIN      
        1 cgatggctgc ttcccacgct ttttcggggg ttattttata agtagttatc ttttttgttt
       61 cgtcagtttg agccattttt ttaccatata gagacaataa aaccacaatg ttacttgtct
      121 tgtggccgaa attgtataaa aaatatttat gattaagttc caaagaactt attattgagc
      181 ttggttcgaa atgtttgctt gtttatcgaa ctgaatttat tctaacatta aggtcttctt
      241 aatattaccg aaaaacaact taaaaaaatc gaattcttta aggttgatca accttaaaga
      301 aacttcatcg cattccgaaa ttatgcgtgt ttcgatcgat tcgcttcttt actttggata
      361 tcatcagcaa aattttctca tattcagaca agaaatcagc tgaggattaa ttcgcataaa
      421 tattagaacg tctattcaaa tcaaaaaaat tttaagaatt acaaagtgaa ttgaaacaaa
      481 taattgccag aaattcgaat tagcaaaaat cattatccga tcatccgggc tacagtattt
      541 cccatcctta ccgcataatt cattccccga tcccaagggt aaatttggct catggatgct
      601 aaatataacc cgttaatcgg agtctctacg gacggaatgt gttcggattg attgataaaa
      661 ggaatcggtt gagcatattt tgccgagaat ttccaactgt ttataaccca atcctcttca
      721 aaattcgggt taattttttt aagtcctggt aaaacccgtt gcaacaactc atcttttgga
      781 agagagaaat attcatgatc ggtctccaga taatctcccg cataaatgat ggtttcgttt
      841 ccgaaacgtt cgcgcggaac aaagttagtg tgctccacta aagaaagaaa aggaaaccct
      901 tcactcttgg ggaggttata ccaataatac ccttcggggg agagaggatg tttcaacgat
      961 aaaaccatta caaccgcgcc catattcttc agatccagta aagactgacg ataatcggcg
     1021 ggtaaatccg gtgatatagc cgaaaaagcc gccggcgata tcgtggcaat aacccgatcg
     1081 aaatcgtcat ttttcccgtc tgcagttact gttatggaag attctttttg tctctttatc
     1141 tgtgtgacat tcgattcaaa acgaatctcc acaccgttcg caatcaactt ttcggaaaaa
     1201 atattgatga aagtctgaaa gccgccgcga aaggttccca gagatgtgga tctggaatgg
     1261 atacgggccc aaagccatgc catgttgact tcttttgcat atttctcacc gaatttccca
     1321 agcatcatcg gttcccacat ggaccggtaa acatgttctc cggcatattt gagcatccat
     1381 tcttccgcgg taactttttc catttctttc catggactga aaagtcgcaa atacaaaccg
     1441 acaaaaccaa aacggatttt gcttcctaag ccgaggccgg ggtaaaggat agcagacggt
     1501 atggaatcaa agggataaaa ctttccccga taatacatga ctgttttagg agatctgaat
     1561 tctaccgaat ttgatacccc gagatcttta aataaagaaa gaatatccga atcaccggtg
     1621 aaaatatgat gataaaagca ttcaacggaa tcctgccatt gaggttcacg aaatccgcgg
     1681 gctaatccgc cgggttcctt ttctttttca aaaatggtca cttcgtgtcc ctgttttctg
     1741 agattccagg ctgcggtcat gccggtcaga cccgatccga taattgctat tttcataaat
     1801 atctccctac cgttttttta atcccatact ttattaaggt ttattttact ctgcgtttat
     1861 cagttccatt ggcagatacg agaatcatcc gttaaaatgc atgtgtcgaa aataatgaag
     1921 cgtaaggaaa atcgtgcccg aacagtcatt cccctttatt tttattcatt tgaatcaaag
     1981 ctgtgatttt atttctcttt ttaattcatt ggtcaaactc ggtggaatac cggttgcttt
     2041 tttgtcggca gaaccggtgc gaaccgaaaa tgaatttatc cgatctgttc ccgtcgttcc
     2101 tttctcagga aataactttc aggaagctct tggaaattta ttatttcact cggaaaaaaa
     2161 taccgggctc gaaagctttt ttgtcatttg ttcggacgat gcggttattt caggttcgga
     2221 attgaatgaa caattggcat cggcccgaaa aaatccgcaa gccttttatc tattggaaat
     2281 cttaacgacc caggaatccg ttgaagataa tgggatcgtc cgaatgattc ggtggattcg
     2341 aaggaaattg gaaggtccga aggcttcctt atggattatc ccgccacaag cgatcacctc
     2401 ggcaaatcca tattccgaag ataaaaagtt ttccttgttc ccgtggctgt tgaaatgtga
     2461 gcgacaaggc atcccgattt ttctgacaca agtaaatcgg aaaaatgacg gggctgttgc
     2521 ggtaaacagc ctccgcagaa tcaacctgct attttataca ttgaatacgt ttttgcgtta
     2581 ttcgggaagt tctgtccttt cattcctcgt agacaatact gttttttttc tgggtataca
     2641 aatcggattc ggcaatcttt tggctttagt caccggccgg gtagcttcgc ttctggtaaa
     2701 ttattttttg ctgaaatccg ttgtttttaa gccgaaaaat agaaagaacc gctcttttgc
     2761 aaaatatgtc ggcttggtga ttttttcagg ctccgttgtt tggagcttga tcactatcgg
     2821 agaaaaaata atccctattc atcccatatt tttgaaattg gcggcggagt tggggatgtt
     2881 tttctttaat tactatgttt caaagacttt tatttttaat taattttttt gcaagaatct
     2941 ttgttgattt tatattcttt cttaataccg acaactttca atcaaatcat cgacgaatag
     3001 acgcaatata attaaaaggg atgaaacgaa aaaaatatgc ggatgaatta accgaagaag
     3061 aattgcgatt ccgtttgatg caaaaaaaaa ccatcggacg agatgctcgt atcgcttcct
     3121 atcgcgccgg cggtcgcgtg atggacgcag cgaatgcgcc gatcaattcc gaattgaaat
     3181 ggaattccga cagatccgaa aaggaagttc tcaaaaccgc accgcctcag aaaaaattcg
     3241 acaaaatatt attggcaatt gagctgctgg cggtggccgg tgtcattgtt atgctggtta
     3301 tcggttctca aattttgcaa aacatcaatc gggaatcggc aaaatctatg attctcccca
     3361 ctctgacacc cactccgctt attcaggctg ttgtcttgcc cggcggtcat actcctccgg
     3421 atgaaagcgg tgtcggtacc tttaatgaaa gcgaaattcc cgagcatttg cgcggtattg
     3481 tttcggtaat ggctcctcaa ccgaccattc ccgccggtat ggaacaggtc attcgcctca
     3541 gaattccggc gattaatgtc gatgcaccgg tcgttttggg ggatgattgg gaagcgttga
     3601 aagcggggat cggaatgaat ctgtacagtg cggctccggg gaaaaacgga aatattattc
     3661 tttccggtca taatgatatt tatggccaag tttttcgcga tttggatcaa ttaatgcccg
     3721 gggatcaaat catcgtatta accgaaaaaa acgcttatac ttatagcgtc caaaacatag
     3781 aagtcgttct gccgaatcag gtggaggtta tggcacagac aacggatccg actgtaactt
     3841 tgatttcttg ctatccttat ttggtagatg ataaaaggat agttgtcacg gggaaaatgc
     3901 tatatataga agaataaatg ctttgggagg gattaatggc aaagaaaaat acgacaaaag
     3961 ggaaggcacc gattaaaaca actgctaccg ccgtttcaag cagcgggaat ctgccgttag
     4021 cggctcgtgc gccgaaagaa gaatttaatc cggattatac ttacgtaatt cgaaatctaa
     4081 agaaaatcgg aacattggca gctgttttta tcgctattct gatcatattg tcattcatta
     4141 tttgatcgaa tcccgcgaat ttttgtttac aaataatcat atgagagacc cgttcggctt
     4201 ttcaaccacg ggtgttttta taaacagaca ataatccaat gccgattaat ttaaatccgg
     4261 ttcccgcacg gattgatccg aaggttcttg cggctcttgc gaagccgcaa agcgggaacc
     4321 ctttctcgct ttcagaaaag attgaagacc gttttcggga gatgttcttt ccggacgccg
     4381 cttctattaa tttttttaat tgttcacggg aagggttgta tgaagttgct ttaaacagtt
     4441 ttgcaagcgg accggtactg atcggaatga acggtccctt ttctcagcaa tggcagacaa
     4501 tcgcaaaatc tttgaatatc gattcggaga tcatcgatac cgctttcggt tccggctttg
     4561 acgaagacct gtttgaaaaa gcacttgatg aggaatccta tgccgctgtt tttttggtcg
     4621 aaattgatcc gtttacgggg acacaaattg atattgccgc attatcggga atgattcgga
     4681 agaagctgcc cgatgctttg gttattgtag attgttccgc ttctttcgga agcgttcgca
     4741 cattagatct gaagtccgaa gcggatgcgt tgttaatcgg ttccgaaata tccttaggtt
     4801 taccgccggg attggggacg gtcattttag gcgaacgagc aaatttcaaa tccatcggtt
     4861 tttcgggaac cggatggagc ctgaactatg caagacaatt ccgtgatatc cattctaaaa
     4921 gttttcgctc tctgatacct tttcaattat taaatgcttt ggacaggcaa cttgatttga
     4981 tttttttgga agggattgaa aaccgaatta aacgaatcgt aagtctatcc gaaaaaatga
     5041 aggggtgggc agatgccaac cggttcgaaa ttttcacacg tccccaattt cgctcaccga
     5101 ccgttacggt cttaaaaaaa ccgcttcctt ttacggttca aaatctgacg gattttttaa
     5161 gctcttacgg cattattatc gggaactgtc cggatgatat gcgggatgat tattttgtaa
     5221 tcgctcatat gaaccagact acggaagaag aaatgacttt atttttggac gttttagacc
     5281 gctttctggc tgattatgat acgaagaaaa gcattccggt tgtcagtaaa ctttcccctc
     5341 aatgataaaa atccgagatt attctttatc gaaatcttct ttaatcacct gaacggtatt
     5401 ggcggctccc aataatacac cgggaattgt cgctcccgga tgagctccgt taccggccaa
     5461 atacagatgg cggatatccc ggcaacgatt tgccattcgg atttccccgt tcttttgtaa
     5521 ttcgggttga aacgacagaa tggatccggc atggctgccc aacctatttt taataataat
     5581 cggatcgaga tattcttcga cgatcagatt tgtccgaata tcggtcagaa aataattttg
     5641 aagcaaatcc aagacatgat ttcgataact gtacgaaata tgatcccagg gattgatact
     5701 ctccaaattg ggaacgggaa cgattacgga gagcgcttca caaccgaacg gcgcattatc
     5761 cgggatgatt ttggaagcca tacgcaggat cagcaatgga tgatccgaaa gggttttttg
     5821 gtcaaaaagt tcatttaaaa atcggtcgta atcttctggg aataagatat ttaaaggagc
     5881 caaaggagct ctttcctgca ggatacaacg ggttccgaga tgataaacaa aggcgctgat
     5941 gccggaaatt tttgtctttg cctgattgac cgaatcatag cgggttcgat cggaatcgat
     6001 taaatatcgg taagtcgcag cggcatccgc atcggaaatt accaaatcgg cttgttgaat
     6061 cctgccgtct tttagtcgta caccggaaac ggtatgattg aatatttgta tttcggatac
     6121 ctcggtccca tagaagatct cgcctcccga ttctcggaac aaccgtacaa tcgcttgaac
     6181 catgttatcc ataccgccgt ccgggactgc tgttccccac cgttgaaaca tttgtgaaaa
     6241 agcccgaaaa agatgggtcg cgcttttggg atttccgccg ccgattaatg gccaaaaacc
     6301 gaaaagttgc tgcagttcgg gagtctgaaa tttttcattt gcatattctt cacaagtctt
     6361 ctgcgggttc attctacgca gccataaatt cgtacgaaat cgaaaagtgt tttcatcaac
     6421 cggtcgaccg caataatcga aaaaaatatc atcgaaaatc gcagcgcttt cctccgtaaa
     6481 agaattaaaa ccttgcaagt ctgccccgga taacataggg ttcagtttta tggtttcggc
     6541 agggttcgaa taaagagcaa agcgcttgaa atcaggaaaa accgcttgga atatcgggtc
     6601 gcaatcccga aatttaaatg aatcgattct gcgtttcccc gcatattcat agagctgatc
     6661 aaataaaaaa ggggcggaga tatattccgc ccccgtatta aacgtaaatc ctttcaaacg
     6721 gattgatccg aggcttccgc ccgcaaaatg gtttttctca aaaattttta ctttgcaacc
     6781 ttcgacagcc agcctgattc cgcaagccag acctcccaag ccggcaccga tgataatgac
     6841 attcagcggt ttcatggaga atcgattaaa tcacctgttg caggacgtca atagcatttc
     6901 gtgcgtttgt catggttgcc tgagttccca tatgtcccaa acggaagacc tttccggcga
     6961 tttttccgta acttccgccg atacccattc cataggttcg aacctgttga tcaaaagctt
     7021 caaaagttcg atcggcggga acatagacgg ccgtaacaga cggtgattga atcgcttccg
     7081 gtttcggaaa cagacggtat ccgatttctg tcaaagattt cctgacaaat tcagcaacaa
     7141 cctgatggcg ggcaaaaaca ttcgacaaac cttcgttgat catcagatca tccaatacat
     7201 catttaatgc cgcaattccc ggccaatccg gtgtattggg gtggtcatgt ttctgcggaa
     7261 tattgtgata agaccaaaat gcttcatacc ccgcataatc aactttccgg ataatatccc
     7321 acgcttgttc gcttaccgaa caaaaaccga tagtgggagg taaagaaagc cctttttgag
     7381 atccgcccat gacaatatcg gcattccatt tgtccccttc aatttctgta caagcaactg
     7441 atgctaccgc atccacgatc attatcggga cattgtattt cttctttaat tgtcccactt
     7501 cagaaatcgg gttaagcgta ccggaaggtg tttcacaatg aacgatcgta atgacctttg
     7561 gttgataggt tttaatggcg gattcgattc gatccaaatc atggacggtt tcatcataac
     7621 caaattcaac cagttgagca tcagcgtgaa tcgcccgtgc catgtctgca aaaccttctc
     7681 cgaaaaatcc ggtactgatt gccaatactt tttcaccggg aagaacacaa cttttaatgg
     7741 caccccagag aatggacatt gcctctccgg attgaataat tacttccgat tgtgtcccga
     7801 gcaaaatgcc cagttttttc ccgacggtct gataaagttc aaagaattca tcttcacaat
     7861 atccggcacc gaaatcagat gtcaaggacc gataagcaac gggagttact cgaaccggac
     7921 ccgggaccat cggaattttg taatttttca ttcgcaggaa ccgtcttcgc agccgctgca
     7981 ggatgaacaa tcatgatgga tgtgtcctgc tttaagttcc ccttcggagg catcccgaac
     8041 ttccactatt tcgatatcaa atttcaattc tttcccggcc agcggatgat tgagatccac
     8101 gataaccgaa ttttcattga aatcctgaat ataagctgtg taaacttggt cggattcatc
     8161 ctgtaaatgt aaaacgcttc cgagagccgg gatgtaatct tctgggaaca aggaacgatc
     8221 caactcaacg attgaatccg gcattaattc gccgtatccg tcttccgggg atacgataac
     8281 ggttttcttt tcgcccggga ataatccgtt taattcattt tcaagaccgg ggataatatt
     8341 gtcatgaccg tgtaaataaa ccaaaggatt atttatatca gccgaatcga tcggttctcc
     8401 gtcaacccat aatgtataag ca
//
LOCUS       DAOMIV010000024         8372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8551_length_8372_cov_4.067693, whole genome shotgun sequence.
ACCESSION   DAOMIV010000024 DAOMIV010000000
VERSION     DAOMIV010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8551_length_8372_cov_4.067693"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /gene="queG"
                     /locus_tag="PKV59_01200"
     CDS             <1..726
                     /gene="queG"
                     /locus_tag="PKV59_01200"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQP45397.1"
                     /translation="VQSIVVLGTPIQPSPLTGTFRIAAFAHYKDYHRQIYSLIEELIR
                     RISEIAGEKPEYKICVDSSPVLERSLAVQAGLGWIGKSSMFISPEAGSLVLLSECLLS
                     WKLPYDNPYPNDRCGKCTRCVDACPTKCIDPQLRTIQADRCISYLTVEHRGDIPAELA
                     KKCGNQIFGCDICTTVCPWNQKTDTGYSPLERFHFSDNLSDDLNLTEEEFKKKYKDTA
                     IYRVKNEGWKRNIKTALQNLYED"
     gene            complement(796..1500)
                     /locus_tag="PKV59_01205"
     CDS             complement(796..1500)
                     /locus_tag="PKV59_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQP45398.1"
                     /translation="MARKNSPQYVIHSYQKKRRVLPYVIWAIAAIILGLGILLLVLWI
                     KGDGNFSLGGLFASATPTSTETFTPSPTVPTSTPTMTPTETVTPSPTISPTPEGPQMY
                     TVEEGDNCWDVAVNKFKVGIDVFLAINGFTMDNCNLTPGQEVIIPGPNTKLPTETPIA
                     LDQYTAGQQIEYKVQLNDSLRDIASKFNSTIESIIKLNKIEDENAPLTAGSTIIIAVN
                     IVTPTPTSVPTYTPVP"
     gene            complement(1600..4497)
                     /gene="uvrA"
                     /locus_tag="PKV59_01210"
     CDS             complement(1600..4497)
                     /gene="uvrA"
                     /locus_tag="PKV59_01210"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQP45399.1"
                     /translation="MSNKNVIRVLGARENNLKNISVDIPRDKLVVITGLSGSGKSSLA
                     FDTIFAEGQRRYVESLSAYARQFLGQMDKPDVDAIEGLSPAVSIDQKGTSKNPRSTVG
                     TITEVYDYLRLLYARIGIPHCPICGREVSKQSSDEIVEAIQKLPEGSRLMILAPIVRA
                     RKGTYQAVLEEIRKSGFVRVRIDGTVYSLDEEVQLDRYKIHTIEAVVDRVIIRHFEDP
                     EEEKMNRSRLADSVETALKVGEGYLTVTNLSSEPAQDMYFSEFLSCPEHGISIPEIEP
                     RSFSFNTPHGACPACQGLGMRLEIDPNLIIPDNSKSINEGAIAVLEWNNSRFDNGYYW
                     QQLLQVASAYDIDLDMPVSKIPNDKMKIILYGSNGASLNMVLTGRNDRKSTYRAPFEG
                     VIPNLERRYNETNSDYIRDRIAEFMKETPCQVCHGKRLKPESLAVTILGKNIIDVTEM
                     SINTTLQWAKDLYEKPGLLTDRQKIIAEKVLKEIISRLDFLVNVGLDYITLQRSAASL
                     SGGEAQRIRLATQIGSQLMGVLYVLDEPSIGLHAKDNAKLLQTLKKLRDLGNTVLVVE
                     HDSETIRSADWIVDMGPGAGDQGGQVVVSGPLNQVLQCRDSLTADYLTGRKFVPIPEQ
                     RRKPDERKLKIIGASENNLKNIDVEIPLGMFVCITGVSGSGKSTLLNDILYKALAHDL
                     NRAMTTPGKYTKIEGTQYLDKIINIDQSPIGRTPRSNPGTYTGVFDEIRSLFAELPES
                     KMRGYKAGRFSFNVHGGRCEACQGQGQLKIEMQFLPDVYVECDVCHGKRFNNETLQVK
                     FKDKSIADILDMTVDDGIEFFSAFPIIQSKLSLLQDVGLGYIRIGQPGNTLSGGEAQR
                     VKLSKELSKRMTGKTLYVLDEPSVGLHAADVHRLIDVLERLVEQGNSVIIIEHNMDII
                     KVADYIIDLGPEGGDRGGKVVTTGTPEEVAENKKSYTGQFLKKVLEQENPG"
     gene            complement(4515..5090)
                     /gene="ruvA"
                     /locus_tag="PKV59_01215"
     CDS             complement(4515..5090)
                     /gene="ruvA"
                     /locus_tag="PKV59_01215"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQP45400.1"
                     /translation="MISYLEGTVIEKESDSITVMVNGIGLKVYVPQSLLGIAEKGKEI
                     LLHTQLIVREDLLALYGFETKEEVEYFSLLMGVSGIGPRLALGILSTVGVDTVRRAVI
                     QDQPDFLSRVPGVGKKTAQKVVLYLQGKISDSFENATLSNLHSADLEAIEALTALGYS
                     LVQAQTAVQSVPKDTPDTVEDKIRAALQSLG"
     gene            complement(5087..5908)
                     /locus_tag="PKV59_01220"
     CDS             complement(5087..5908)
                     /locus_tag="PKV59_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQP45401.1"
                     /translation="MMITSTSNSKIKQIRQIQDKKQQKKSNLFYMEGLRIIGEAYKTE
                     NLLETLIFCPELLKSEYGNQLIEQGKQTGLEILEVSESVFKSFSIKENPQGIAAIGIK
                     KEYHLDDLNTKKGIIVALTEIADPGNLGTIIRTSDAVDANGIVLLGDSVNPYESGSVR
                     GSMGALFSQKIIQTDFETFLAWKNEKKIKMVGTSDHTETDYLEISYPNPIILLMGSER
                     QGLTREQIDSCDFMARIPMLRSSDSLNLAVATGIMLYQIYNCHRGYVLTSEKGNA"
     gene            complement(5908..6405)
                     /gene="ruvC"
                     /locus_tag="PKV59_01225"
     CDS             complement(5908..6405)
                     /gene="ruvC"
                     /locus_tag="PKV59_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQP45402.1"
                     /translation="MVIIGVDPGTALTGYGFIDLSDRGELSVIDYGVICTPAKDKMEK
                     RLLQLYSEINEKILLYGPQRGAVEKLFFHSNVTTAISVGQARGVILLSLARAGIDVDE
                     FTPMEVKQAVVGYGGAEKRQVQMMVKAILKLNEMPKPDDAADALAIAICCANTNRFPN
                     ILESF"
     gene            complement(6413..7168)
                     /locus_tag="PKV59_01230"
     CDS             complement(6413..7168)
                     /locus_tag="PKV59_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP45403.1"
                     /translation="MSGHSHWATIKRKKGAGDAKRGQVFTKLAKEITMAAREGGDPNM
                     NFKLRLAVDKARESNMPKDSIERAIKRGTGESKDGAVFEDVMYEGYGPKGVALMVECV
                     TENRNRTVADVRHALSRAGGNLGEAGSVAWQFSRKAYFLLTGDNLDFDKIFEIALENG
                     ADDVEQSEDGIEIYAEADAYKMMSDALFAAGLHIQEAQIRMIPNQPIDLPLDDTLQVL
                     RVIDALEELDDVQQVYHNMNISDEAIAALENEE"
     gene            complement(7355..8125)
                     /locus_tag="PKV59_01235"
     CDS             complement(7355..8125)
                     /locus_tag="PKV59_01235"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQP45404.1"
                     /translation="MKFIRYIQDNKPEVKIGWIYEDMVGGIEGDMFSDYRRLEATLPI
                     DRVKLLAPLIPGKIVAVGWNYQDHAKEFDRPVPEYPTIFLKPPSAVIGTGDGIVLPPT
                     SERVEHECELALVIGKKAKMVLPEEAFSYIFGYTVANDVTARDLQKKDDTWTRSKGYD
                     TFCPLGPWIETDFEPADAMLTCRVNSELRQMASTRDMVFSIPAIIAYISSIMTLNPGD
                     VILTGTPAGTSPLKEGDILETQIEGIGRLVNPVVKQTK"
     gene            complement(8139..>8372)
                     /locus_tag="PKV59_01240"
     CDS             complement(8139..>8372)
                     /locus_tag="PKV59_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HQP45405.1"
                     /translation="MDAFASVISNNLNVVMKLLASVTIILSIPNVISGFFGMNINFPD
                     VIETHESTMIIIFGIIVLFTFFAIYIFQKKNWF"
BASE COUNT         2322 a         1974 c         1526 g         2550 t
ORIGIN      
        1 gtgcaatcaa ttgttgtttt gggcacaccg attcagcctt cacctttaac cggaactttc
       61 agaatcgcgg cattcgcaca ttataaagat taccatcgtc aaatttattc attaatcgaa
      121 gagctgattc gacggataag cgaaattgcg ggagaaaaac cggaatacaa aatatgcgta
      181 gattcatccc ctgttttgga acgctcattg gctgttcaag ccggtttggg ttggatcgga
      241 aaaagttcaa tgtttatttc gccggaggcg ggatctttgg ttctgttatc agaatgcctt
      301 ttatcctgga aactgcctta tgataatccg tatccgaatg accggtgcgg taaatgcaca
      361 cgatgcgtag atgcctgccc cacaaaatgt atcgaccctc aattacgaac tatacaagcg
      421 gatcgttgta tatcctatct aaccgttgaa cacagggggg atattcctgc ggagcttgca
      481 aaaaaatgcg gtaatcaaat tttcggttgc gatatatgca ccactgtttg cccttggaat
      541 caaaaaacag ataccggcta ttctccatta gaacgatttc atttctcaga taatctttcg
      601 gatgatttga atctcaccga agaggaattt aaaaagaaat ataaagatac tgccatctat
      661 cgtgtgaaga atgaaggatg gaaacgaaat atcaaaacag cacttcaaaa tctctatgag
      721 gattaaaagg aaggaattcc tgacaaaata gctccgccat gtttgacgga gctattttgt
      781 cattgtctct aaaatttatg gtacaggtgt atatgtcgga acggaagtgg gtgtcggtgt
      841 tacgatattg actgcaatga taattgtgga gcctgcggtt agcggagcat tttcatcttc
      901 gatcttattt aacttaataa tcgattcaat tgtggagtta aattttgatg caatgtctct
      961 cagagaatca ttcagttgaa ctttatattc aatttgttgg cctgctgtat attgatcaag
     1021 cgcaatcggt gtttccgtag gtaatttcgt attgggaccg ggaataataa cttcctgtcc
     1081 gggtgttaaa ttacaattat ccatcgtaaa cccgttaatt gccagaaaaa catcaattcc
     1141 gactttaaat ttatttacgg ccacatccca acaattatca ccctcttcga ctgtatacat
     1201 ttggggtcct tcaggtgtcg gagaaattgt cggtgacgga gtaactgttt cggtaggtgt
     1261 catagtcggc gtggaagtcg gaacggtcgg tgaaggtgta aaggtttctg tggaagtggg
     1321 cgtcgccgaa gcaaataagc ctccgagtga gaaattcccg tctcctttaa tccataacac
     1381 taataataaa atccccaagc ctaagataat cgcagcaatt gcccatatta catatggcag
     1441 cacccttcgc tttttttgat atgaatgaat cacatattga gggctatttt ttcgtgccat
     1501 tgtgcatttc ctttcaccgt catcgatcaa cggatttcct tctcttgagt tattttttat
     1561 tttacacggt attcacattc tttgcaaaat atatagaaat tatcccgggt tctcctgttc
     1621 caaaactttt ttcaaaaatt gtcccgtata tgatttttta ttttctgcga cttcctccgg
     1681 tgtaccggtc gtaacaacct ttccgcctct gtcgccgcct tcgggtccca gatcaatgat
     1741 ataatccgcg actttaatga tatccatatt atgctcgata ataatgacgg aatttccttg
     1801 ttcaaccagc cgttccaaaa catcaatcaa acgatggaca tcggcagcat gaagccccac
     1861 agagggttca tccaaaacat ataatgtttt tccggtcatt cgcttggata gttctttgga
     1921 aagtttgacc ctttgtgctt caccgcctga aagtgtattt cccggttgcc cgattcggat
     1981 ataccccaga ccgacatcct gtaacaggga taatttgctt tgaataatcg ggaaagccga
     2041 aaaaaattcg atgccgtcat caaccgtcat atccaaaata tccgcgatgg atttgtcttt
     2101 aaatttcacc tgtaacgttt cattattaaa ccgtttcccg tgacaaacat cacattctac
     2161 ataaacatcc ggtaaaaatt gcatctcaat tttcagttgc ccttggcctt gacacgcctc
     2221 acaacgcccc ccgtgcacat tgaaagagaa tcggccggct ttatatcccc tcattttgct
     2281 ttccggaagc tcagcgaaca gacttcgtat ttcatcaaaa acgcccgtat aagtccccgg
     2341 atttgatcgg ggagtcctgc cgatcggtga ctgatcgata ttgatgattt tatcgagata
     2401 ttgagtgcct tcgattttcg tatattttcc gggagttgtc atcgcccgat tcagatcatg
     2461 tgccaaggct ttatacaata tatcattcaa caatgttgat tttccgcttc cggaaactcc
     2521 cgtaatacat acaaacattc ctaagggaat ttcaacatcg atgtttttaa gattattctc
     2581 actcgccccg atgatcttca gttttctttc atccggtttc cgcctttgtt ccggtatcgg
     2641 aacaaatttc cttccggtca gataatctgc ggttagtgaa tcccgacatt gcaagacctg
     2701 atttaatggc cccgatacaa caacttgtcc tccttgatct ccggctccgg gtcccatatc
     2761 aacaatccaa tctgcggatc gaatggtttc cgaatcatgc tccaccacga ggactgtatt
     2821 ccctaaatct cgtaatttct tcaatgtctg cagtaatttt gcattatctt ttgcatgtaa
     2881 accgatagaa ggctcatcca atacatacaa tacacccatt aattgagatc cgatttgagt
     2941 cgccaaccga atgcgttgcg cctctccgcc cgaaagactt gccgcggaac gttgaagtgt
     3001 aatgtaatcc agaccgacat ttacaaggaa atccagtcgt gaaataatct cttttaatac
     3061 cttttccgca atgattttct gacggtccgt caacaatccg ggtttttcat ataaatcctt
     3121 ggcccattgc aaagtcgtat tgatcgacat ctcggtaaca tcgatgatat ttttaccgag
     3181 aatcgtcact gccaaagatt ccggtttcag tcgtttcccg tgacaaacct gacagggagt
     3241 ttctttcata aattcagcga ttcgatctcg aatatagtct gaattggttt cattgtatcg
     3301 gcgttccaaa ttgggaatca caccttcgaa tggggctcga taagtcgatt ttcgatcatt
     3361 tcgtccggtt aaaaccatat tcaagctggc tccattggaa ccgtataaaa tgattttcat
     3421 tttatcattc ggaatctttg aaacgggcat atccaaatca atatcgtatg ccgaagcgac
     3481 ttgtagaagc tgttgccaat aataaccgtt atcaaatctt gaattattcc attccaaaac
     3541 agcgattgcg ccctcattaa tcgatttcga attatcggga ataatcagat tcggatcaat
     3601 ctccagccgc attcccagcc cttgacaagc cggacatgca ccatggggcg tgttaaacga
     3661 gaaagatctc ggttctattt ccggaatgct aatcccatgt tccgggcaag atagaaactc
     3721 cgaaaaatac atgtcttgtg ccggctccga agagaggttg gtgacggtta aataaccttc
     3781 gccgaccttt aatgctgtct ctaccgaatc ggccaacctt gaccggttca ttttttcctc
     3841 ttccggatct tcaaaatgtc ggataatcac ccgatcaact accgcttcga tcgtgtgaat
     3901 cttatatcga tccagttgaa cttcctcatc caatgaataa accgtcccgt cgatccgcac
     3961 ccgaacaaaa ccgctttttc tgatctcttc caaaacagcc tgataagttc cctttcttgc
     4021 acgaacaatc ggagcaagaa tcatcaaacg gctcccttcc ggcagctttt gaatagcttc
     4081 aacaatttca tcggaggatt gtttgcttac ttctcttcca cagatcggac agtgcggaat
     4141 accgatcctt gcataaagca aacgaaggta atcataaact tccgtaatcg ttcccacggt
     4201 tgaacgcgga tttttcgaag ttcctttttg atcgatcgaa actgccggtg ataagccttc
     4261 aatcgcatca acatccggtt tatccatctg gcctaaaaat tgtctggcat aagcggataa
     4321 agattccaca tatcgccttt gcccttcggc aaaaatagta tcaaaagcta acgaagattt
     4381 tcccgatccg gaaagaccgg taatcacaac caatttgtct ctgggtatat caactgaaat
     4441 attttttaaa ttattttccc gtgcgccaag cacacggatc acatttttat ttgacacaaa
     4501 caaactccca tgaatcagcc caacgattgc agggctgctc tgattttatc ttctacggta
     4561 tccggagtat ccttagggac agattggaca gcggtttgcg cttgtaccaa actgtaacct
     4621 aatgctgtca gcgcttcaat cgcttctaaa tcggcggaat gaagattgct caaggttgca
     4681 ttttcgaaag aatcggatat tttcccctgt aaatataaaa caactttttg tgcggttttt
     4741 ttccccactc ccgggactct tgaaagaaaa tccggttgat cctgaatcac agcacgtctg
     4801 acggtatcaa ctcctactgt cgagagaatt cccaaagcta atcgtggccc gattccgctg
     4861 acgcccatca ataaggagaa atattcaact tcttcttttg tttcaaatcc gtataatgcc
     4921 aataaatctt cgcgaacgat caattgtgta tgaagaagaa tttctttccc tttttcggcg
     4981 ataccgagca agctctgcgg aacataaact ttcaatccga tcccattcac catgacagta
     5041 attgaatcgg attctttttc gattacggtg ccttccaaat acgaaatcat gcatttccct
     5101 tctctgaggt taacacatac cctcgatggc aattatatat ttgatatagc atgatacccg
     5161 tcgcgacagc taaattcaat gaatcgctgg atcttaacat cggaatacga gccataaaat
     5221 cacaagaatc gatttgttct cgagtcagac cttgtctttc gcttcccatt aataaaatta
     5281 tcggattcgg ataagaaatt tccaaataat ccgtttcggt atgatccgag gttccaacca
     5341 tttttatttt tttttcattt ttccaagcca aaaaagtttc gaaatccgtt tgaatgatct
     5401 tctgagaaaa caaagctccc atcgaccccc ggaccgatcc cgattcgtac ggattgaccg
     5461 aatcacccag cagaacaatc ccattcgcgt caaccgcatc cgatgtccga ataattgttc
     5521 cgagattccc gggatcggct atttcggtta atgccacaat gattcctttt tttgtattca
     5581 aatcatccaa atgatattct ttctttatgc cgattgctgc gatcccctga ggattctctt
     5641 taattgaaaa cgatttaaaa acactctcac tgacttctaa gatttccaac cctgtttgtt
     5701 ttccctgttc aattaattga ttcccgtatt cactttttaa taattccgga caaaaaatga
     5761 gcgtttccaa caagttttcc gttttatatg cttctccgat aattcgaagc ccttccatat
     5821 aaaacaggtt ggattttttt tgttgctttt tatcctgaat ttggcggatt tgttttattt
     5881 ttgaattgga agtacttgtt atcatcatca gaaagattcg agtatattcg gaaatcgatt
     5941 cgtattcgca caacaaattg cgattgccag agcatcagcc gcatcatccg gtttcggcat
     6001 ttcattaagt ttcagaattg ctttaaccat catttggact tgacgtttct ctgcgccacc
     6061 gtaaccgaca accgcttgct tcacttccat cggagtaaat tcatcaacat ctatgccggc
     6121 ccttgccaaa cttaaaagga taacgcctct ggcttgtccg acactgattg cggtagtaac
     6181 attgctgtga aaaaacaact tctctacggc acccctttga ggtccataga gaagtatttt
     6241 ttcattgatt tctgaataaa gttgcaggag tctcttttcc atcttatctt tcgccggtgt
     6301 gcaaataacc ccgtaatcga ttacggataa ttcacctctg tcagataagt caataaagcc
     6361 atatcctgtc aatgccgtgc caggatcgac acctatgata accatgcaac tattattctt
     6421 cgttttctaa agctgcaatt gcctcatcac tgatattcat attatgatag acttgttgaa
     6481 catcatccaa ttcttccaat gcatcaatga cacgaagtac ctgtaatgta tcatccagag
     6541 gaagatcgat cggttgattc ggtatcatgc gaatttgcgc ttcttgaata tgcaagcccg
     6601 ccgcaaataa agcatcgctc atcattttat aggcatcagc ttccgcataa atttcgatac
     6661 cgtcttcact ttgttcgaca tcatccgcac cgttttcaag cgcgatttca aaaatcttat
     6721 cgaaatcgag attatctccg gtcagaagaa aatatgcttt tcgcgaaaat tgccaggcaa
     6781 cggatcccgc ctcacctaaa ttaccacccg ctcgagacaa agcatgtcga acatcggcaa
     6841 cggttcggtt cctattctct gtcacacatt caaccattaa agcaacgcct ttcggtccgt
     6901 aaccttcgta cattacatct tcaaaaactg cgccgtcttt tgattctccc gtaccgcgtt
     6961 ttattgctcg ctcgatactg tctttcggca tattactttc ccgcgcctta tcgacagcga
     7021 gccgcagttt gaagttcata ttcggatctc caccctcgcg cgctgccatg gttatttctt
     7081 tcgcaagctt cgtaaaaacc tggccgcgct tcgcatctcc cgcacctttc tttcgtttta
     7141 tcgttgccca atgagaatgg cctgacatat tttcattccc cttttctgaa tggaatcatt
     7201 ctttatgaca gaatgttttt taatttcgat cgttcatttt gctttttatc taatatacaa
     7261 atgaccaaat aattatacac ttattatgag ttgcacagat atccttgctt caccaaacat
     7321 gaactttaat ctattaaatc cggactgata ccaattattt cgtttgtttt accactggat
     7381 tgactaatct gccgattcct tcgatttgag tttcgagtat atccccttcc ttcagaggag
     7441 aagtcccagc cggcgtcccg gttaaaataa catcgcccgg atttaaagtc ataatggatg
     7501 aaatataagc gataattgca ggaatcgaaa aaaccatatc acgggttgaa gccatttgtc
     7561 tcagttcgga attaacacga caggttaaca tagcatccgc cggttcaaaa tccgtttcaa
     7621 tccatggccc taacggacaa aaagtatcgt atcctttact acgagtccaa gtatcatctt
     7681 tcttttgtaa atctcgagct gtaacatcgt ttgcaacagt gtatccaaag atataggaaa
     7741 aagcttcttc cggcaatacc attttcgctt ttttcccgat cactaaagcc aactcgcatt
     7801 catgttccac tcgctcggaa gtcggcggaa ggacaatccc gtcaccggta ccgattaccg
     7861 cactgggagg ttttagaaaa atagtcggat actccgggac aggccgatca aattcctttg
     7921 catgatcttg ataattccaa cccaccgcaa caatttttcc gggaatcaaa ggtgcaagta
     7981 gttttacacg atcaatcgga agtgttgctt caagacgacg ataatcggaa aacatatcgc
     8041 cttcaatacc gcctaccata tcttcataga tccaaccgat ttttacttca ggcttgttat
     8101 cctgaatata acggataaat ttcattctgc acccctttct aaaaccaatt ctttttctgg
     8161 aaaatataaa tcgcaaaaaa agtaaataaa acaatgatcc caaatataat aatcatggta
     8221 gattcatgcg tttcgatgac atccggaaaa ttgatattca tgccgaaaaa tccgctgata
     8281 acattcggaa tgcttaatat aatcgttaca gatgccaaca atttcatgac cacattgaga
     8341 ttgttggaaa tcaccgaagc aaacgcatcc at
//
LOCUS       DAOMIV010000025         8290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8670_length_8290_cov_4.364299, whole genome shotgun sequence.
ACCESSION   DAOMIV010000025 DAOMIV010000000
VERSION     DAOMIV010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8670_length_8290_cov_4.364299"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PKV59_01245"
     CDS             <1..1601
                     /locus_tag="PKV59_01245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP45406.1"
                     /translation="FQYGVFIFFAVYLAIGLFVFDDYGCGPDEGMERQTSLVNYKYVI
                     EKLNLPVSEPVKTWLGYLPPLKEYRDRYYGTAVQIPMVLIESSSAFTMEPAEFYGIRH
                     LYTFLNFFVAMICFYLLLKERFKHRGIALLGTAMLILTPRFFAESFYNNKDVLFLSWY
                     IISIYFIFRWFRKKTTGAAIVAGILLAFTINTRLNGIVLIPLVILFAVIEQFEKKYPF
                     REAMLPLCILIVTCLVTFFIITPNYWESPVSTFLEAFAFNMKHPNHGSDGNLFFGNLV
                     DSTKIWYFIPTWIVLTIPTCIVLLSVLGFGKIIIDFIRNRFSFFEHNELFMDLFMTAA
                     GLIPLLVIIFMRVIIYNGWRHCYFFYGTLIYLTVVGLQTVWEIKIKHPTFQFLKNALL
                     ILGIGSLLFGNGLWIIRNHPFEYVYFAPLFRKYSDEFSGDYWGIASRNLLEYIVQTDD
                     RPQIIVDHSLTNAGSINRGLLPEKDRDRLDLVYDTENADYIIFSRDDKTADQASFTGF
                     KKVYSIRVDSDEIGIVFKRETEIN"
     gene            1607..2299
                     /locus_tag="PKV59_01250"
     CDS             1607..2299
                     /locus_tag="PKV59_01250"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HQP45407.1"
                     /translation="MPICYIVGAAEMDQTFHPEETDFTIAADAGLRHFRRLRIQPDLI
                     IGDFDSLGSVPPQPQVVCFPPEKDDTDMMLAIKAGLKKGYVDFRLYGGLGGRLDHTLA
                     NIQALLYLAHHQAQGFLIGEGMVLTAITNGSLRIKEGKKGILSVFCFGEPATGINLTG
                     LKYPLHDATLTSDMALGISNEFTDNPAEISVKNGTLVILWFDERFDDQSVSIIRFPRN
                     KDHSSASSFRTL"
     gene            complement(2358..3617)
                     /gene="ahcY"
                     /locus_tag="PKV59_01255"
     CDS             complement(2358..3617)
                     /gene="ahcY"
                     /locus_tag="PKV59_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQP45408.1"
                     /translation="MTDFDIKNDSLAEGGRRRIEWAEREMPVLRLIKERFQKEKPLKG
                     IRMSACLHVTAETANLMKTLQAGGADVVLVASNPLSTQDDVAAALVIHDEIPVHAIKG
                     EDNATYYKHIEAALAHKPQITMDDGADLVSTIHKSHRDLIPNIYGGTEETTTGVIRLR
                     AMAADKALMFPVIAVNDAMTKHFFDNRYGTGQSTLDGIIRATNILIAGKVFVVCGYGW
                     CGRGLAQKACGMGADVIVTEVDSLKALEAVMDGFRVMPIKEAAKAGDIFCTVTGDLNV
                     IDKEHFESMKDGAIVANSGHFNCEINIPALESLSVGKKLVRPFVEQYELKDGRKIHIL
                     AEGRLINLSAAEGHPASVMDMSFANQALSAEYMVKNHMTLEKKVYSVPEEIDREIAAL
                     KLKAMGVRCDELTEEQKNYLNSWESGT"
     gene            complement(3780..4784)
                     /locus_tag="PKV59_01260"
     CDS             complement(3780..4784)
                     /locus_tag="PKV59_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQP45409.1"
                     /translation="MKRISILICSVLLLTAMVLSGCSTNTEKATATVKATVESVATVS
                     KVETAKDAASEAATTEKTAATGSAVTTEETVKETAVTFSKENPITLKIGASPVPHAEI
                     LAQIKEPLAEKGINLEVVEFTDYIQPNIALEDGSLDANFFQHFPYLDDFNKNQKKENA
                     DWTTLVPLVYVHFEPMGIYKGKTASLDALKEGAKIAVPNDPTNEARALLLLEANGLLK
                     IAEGKGLEATAKDIVDNPKKFEIVELEAAQVALSIQDVDIAVVNGNYALSSGLTQADI
                     LAQESSDSEAAKKYANLVAVREGEEDLPQFVLLKEAITSDAVKKFIEEKYKGSVAPVF
                     "
     gene            complement(4870..5559)
                     /locus_tag="PKV59_01265"
     CDS             complement(4870..5559)
                     /locus_tag="PKV59_01265"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HQP45410.1"
                     /translation="MSFFTYLVNLLSEFHQLLIDATLQTLYITFISTVLAYLLGTPLG
                     ILVVITAPKSIAPHKGVNEVLSWIINIGRSIPFIILMVALMPLTRWIVGTTIGPVATI
                     VPLTIAATPFVARMIEQSLVEVDSGLIEMAQAMGATSSQIIFKVLLPEGLPSVVRGIP
                     ITIINLIGSYAMAGAIGGGGLGDLAIRYGYHRRQSDIMIVTIVLLIIIVEIIQLLGNF
                     VANRIDKRDVR"
     gene            complement(5564..6346)
                     /locus_tag="PKV59_01270"
     CDS             complement(5564..6346)
                     /locus_tag="PKV59_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP45411.1"
                     /translation="MSKQFEMKDGPFQALEDVSLHVEQEDIYGIIGMSGAGKSTLIRC
                     INLLEEPTWGEILIEGKCIFRRLKDVSDPKAEDQKFVRLNDTDLRKLRREIGMIFQSP
                     NLLMQRTVEENIAFPLEIAGYPRSKIQDRVKELMDLVNILDKAHNYPSQLSGGQRQRV
                     SIARAMANTPKILLCDEPTSALDSLTTTQILDLLWDINQQYKVTIVIITHEIELVKKL
                     CSKVAVIDNTRIVDQGEVGQVLLNPSMEITRQLMRGKIGSEE"
     gene            6830..7177
                     /locus_tag="PKV59_01275"
     CDS             6830..7177
                     /locus_tag="PKV59_01275"
                     /inference="COORDINATES: protein motif:HMM:NF023127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQP45412.1"
                     /translation="MDKINENEKEYRFGDWGPKYIIKGPRMNMGIIRLKPGQKLTPHF
                     HKVMEENFYVEKGNVQLNVDGTVYNLIPGDVVRCEPNEVHALANVSSEEARVIFMLAP
                     YMESDKFEVEETK"
     gene            7529..>8290
                     /locus_tag="PKV59_01280"
     CDS             7529..>8290
                     /locus_tag="PKV59_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HQP45413.1"
                     /translation="MIHTTITAHSGCDDTPMNSLESVLSGINDRADIIEVDVRADKNG
                     RLILSHNLDQERIYAGHAELIQVLEIFQTNKNICLNCDIKETEIIPSVLESAEQYQLS
                     KDRIYLSGSVTPELLRQRTEIIEKCDIQWNIEEAMIPLVEKKLIAERKEYLRETLHTN
                     PWEVLRDEVPDPDRYLPDLIKMAKDFSLTTFNMPLEYATDDRIRFFHNNGLKVSVWTV
                     NNSEEIYRLLRLRVRNITTKKVKMAIKVKMEFLGFT"
BASE COUNT         2314 a         1868 c         1657 g         2451 t
ORIGIN      
        1 tcttccagta tggggttttc attttttttg cggtctatct cgcaatcgga ttgtttgttt
       61 ttgatgatta tggctgcggt ccggacgaag gaatggaacg ccaaacatct ttggtcaatt
      121 acaaatatgt cattgaaaaa ttaaatcttc cggtcagtga accggtaaaa acctggttgg
      181 gatacctccc gcctttgaaa gaatatcgcg atcgttatta tggcaccgct gttcaaattc
      241 cgatggttct catcgagagt tcttccgcct ttaccatgga acctgccgaa ttttatggta
      301 tccgccacct gtacactttt ctcaatttct ttgtcgccat gatttgcttt tatttgttat
      361 taaaagaacg cttcaagcac cgaggaatcg cacttttagg gacggcaatg ctgatcttga
      421 ctccccgttt ttttgccgag agcttttaca acaataaaga tgtattgttc ctttcttggt
      481 atatcatttc gatctacttc atttttcgtt ggtttcgcaa gaaaacaacc ggcgcggcta
      541 tcgttgccgg tattttactt gctttcacga tcaacacccg cttaaacgga atcgtgctca
      601 tcccgttggt tattcttttc gccgtcattg aacaattcga aaaaaaatat ccgttccgag
      661 aagcaatgct ccctttgtgc atcctgatcg tcacctgttt agtcactttt ttcatcatta
      721 cgccaaacta ctgggaaagt cccgtttcca cttttctcga agcctttgca ttcaatatga
      781 aacatcccaa tcacggctca gacgggaatt tattcttcgg caacttggtt gattcaacca
      841 aaatatggta ttttattccg acatggattg tcctaaccat tccaacctgt atcgttcttt
      901 tatccgtctt gggattcgga aaaataatca ttgattttat ccgaaatcgc ttttcgtttt
      961 tcgaacataa tgaactcttc atggatttat ttatgacagc tgccggactg atccccctct
     1021 tggtcatcat tttcatgcga gtgatcattt acaacggttg gaggcattgt tatttctttt
     1081 acggtacact catctatctg acagttgtcg gtcttcagac ggtttgggaa ataaaaatca
     1141 aacatccgac ttttcaattc ctaaaaaatg ctttacttat cttgggtatc ggatcattgc
     1201 tattcggcaa cggactatgg atcataagaa atcatccttt tgaatatgta tatttcgctc
     1261 cgcttttccg caaatactcc gacgaatttt cgggagatta ttggggaatt gccagtcgca
     1321 atctgttgga gtatatcgtt caaacggatg accgtcctca gatcatcgtc gatcacagcc
     1381 tgacaaatgc cggcagtatc aatcgcggat tgttgcctga aaaggatcga gaccgtttgg
     1441 atttggttta tgatactgaa aatgccgatt atattatctt cagcagagat gataaaaccg
     1501 cggaccaagc atcgtttacg ggatttaaaa aagtctattc gattcgagtc gattccgacg
     1561 aaatcggaat cgtattcaaa cgggaaacgg aaatcaacta aatcttatgc ctatatgtta
     1621 cattgtcggt gcggctgaaa tggatcaaac attccatccg gaagaaacag atttcaccat
     1681 tgcagccgat gcgggcttga ggcatttccg gcgattgagg attcaacccg atctgatcat
     1741 cggtgatttt gattcactgg gttcggttcc gccgcaacct caagttgtct gttttccccc
     1801 ggaaaaagat gatacggata tgatgctggc tatcaaagcc ggtctcaaaa aggggtatgt
     1861 tgatttccgc ctttacggcg gtttgggcgg cagattggat catactctcg caaatatcca
     1921 agctcttcta tatcttgctc atcaccaggc acagggattt ttaatcggcg agggtatggt
     1981 tctgacggca ataaccaacg gttcacttcg tattaaagag ggcaagaaag ggatcctatc
     2041 cgttttttgt ttcggagagc ccgccacagg tatcaatctg acaggcttaa aatatccact
     2101 gcatgatgca acgttgacaa gtgatatggc tctcggaatc agcaatgaat ttacggacaa
     2161 tcccgcggaa atttcggtta aaaacggcac tttggttatt ctttggttcg atgagcgttt
     2221 cgatgatcag tcggtttcga tcattcgctt tccgagaaat aaagatcatt catccgcttc
     2281 atccttccga accctttaaa taaataaacc tgaggaaatc ctcaggttta tctatgcgaa
     2341 atgaataata ctgtgtttta tgttccggat tcccaagaat tcagatagtt cttttgttct
     2401 tcggttaatt catcacaacg cacgcccatt gctttcagtt tcaacgccgc aatttcgcga
     2461 tcgatttctt ccggaacaga ataaactttc ttttccagag tcatatgatt tttaaccata
     2521 tattccgccg acagagcttg gttagcaaaa gacatgtcca ttaccgacgc cggatgacct
     2581 tccgccgcag ataaattgat taatcttcct tctgccaaaa tatgaatttt tcttccgtcc
     2641 ttcaattcat actgttcaac aaatgggcgg accagcttct ttccgaccga taaactttca
     2701 agtgcgggga tattgatttc gcaattaaaa tggcccgaat tcgcgacaat cgccccgtct
     2761 ttcatcgatt cgaaatgttc cttatcaata acgttaagat ctcctgttac ggtgcagaaa
     2821 atatcgccgg cttttgctgc ttctttaatc ggcatgaccc ggaaaccatc cattaccgct
     2881 tccaaagctt tcaaagaatc cacttcagtc actatgacat ccgcacccat accgcatgcc
     2941 ttctgtgcaa gtcccctgcc gcaccaacca taaccgcaca ccacaaaaac tttcccggct
     3001 atcaaaatat tcgtcgcgcg aataatgccg tccagagtcg attgcccggt tccgtaccgg
     3061 ttatcaaaga aatgttttgt catggcatca ttgacagcga taaccggaaa catcaacgct
     3121 ttgtcggcag ccatagctcg cagccggata acgccggttg ttgtttcctc cgtaccgccg
     3181 taaatattcg gtattaaatc acgatgactt ttatgaatcg tgctgaccaa atcagccccg
     3241 tcatccatcg tgatttgcgg tttatgagcc aaagcagctt cgatatgttt atagtaggtg
     3301 gcattatcct cacctttgat cgcatgaacc ggaatttcgt catggatgac caaagcggcg
     3361 gccacatcat cttgtgtgga taacggattt gatgcgacca gcaccacatc cgctcctccg
     3421 gcttgcaaag tcttcatcaa atttgcggtt tccgccgtaa catgcagaca agctgacatc
     3481 cgaattccct tgagcggttt ctccttttgg aatcgttcct taatcaaacg aaggaccggc
     3541 atttctctct ctgcccattc aatccggcgg cgtccgcctt ccgccaacga atcatttttg
     3601 atatcaaaat cagtcatgaa agctccttaa ttgttttatt gacaatagat tttactatga
     3661 tttacgttca attttcggta aaaggctcaa tcggattatt gaaatcggtt ttttgcttta
     3721 aacaaaacgg ccggtctttc caaaccggtc gttttattgg ttcaaactct tgcgataaat
     3781 cagaatacgg gagcgacgga acctttatat ttctcctcaa tgaatttctt cacggcatct
     3841 gaagtgatcg cttcttttaa caatacaaat tgcgggagat cctcttcgcc ttcgcgaacc
     3901 gcaaccaaat tggcatattt tttagccgct tcggaatcgg aagattcttg agcaaggata
     3961 tccgcttggg ttaagccgga acttaatgca tagttgccgt ttacaaccgc aatatcgaca
     4021 tcttgaatgg acaaggccac ctgcgcggct tccaattcga cgatttcaaa cttcttggga
     4081 ttgtcgacaa tatctttcgc cgtggcttcc aaacctttcc cttccgcaat tttgagcaga
     4141 ccgtttgctt ccagtaggag caaagcccgc gcttcattgg tgggatcatt cggaacggcg
     4201 attttcgcgc cttcttttaa tgcatccaaa ctggcggtct tccctttata aatgcccatc
     4261 ggttcaaaat gtacgtaaac taacggaaca agagtggtcc aatccgcatt ctcttttttc
     4321 tgatttttat tgaaatcatc cagataaggg aaatgctgga agaaattagc atccagactg
     4381 ccgtcctcaa gagcaatatt cggttgaata tagtccgtaa attcaaccac ttccagattg
     4441 atcccttttt cggctaacgg ctctttgatt tgagctaata tttccgcatg cggaaccggt
     4501 gatgcaccga ttttaagggt gatcggattt tctttggaaa aagtaaccgc tgtttcctta
     4561 accgtttctt cggttgtcac agccgaaccg gttgctgcgg ttttttctgt cgttgctgct
     4621 tcggaagcag catcttttgc ggtttcaact ttagaaacag tcgcgacact ttcaaccgtg
     4681 gctttcacgg ttgccgtcgc tttttccgta ttcgttgagc aacccgacag aaccattgct
     4741 gttaacagca atacactaca aataagaatg ctaatacgtt tcatgatatt gctccttcta
     4801 aaacaaattc acttatttga ataagaaaga tcgaacggaa atccgctctc tcttttttcg
     4861 tacaacagat caacggacat cccgtttatc aatccgattc gcgacaaagt tacccagtaa
     4921 ttgaatgatt tccacgatga taatcaacaa gactattgtg acaatcatga tatcagactg
     4981 ccggcggtga tatccgtatc ggatcgccaa atctcccagc ccgcccccgc cgatggcacc
     5041 ggccatggca taggatccga tcaaattgat aatagtgatg ggaatgccgc gaaccaccga
     5101 aggcaatcct tccggcaata gcactttaaa gataatctgt gaggaagtag cgcccatggc
     5161 ttgggccatt tcaatcaagc cggaatccac ttcgaccaag gattgttcga tcatgcgcgc
     5221 gacaaaaggc gtcgccgcta ttgtgagcgg aacaattgtc gcaaccggtc cgattgtcgt
     5281 tccgacgatc caccgggtaa gcggcatcag agcgaccatt aggataatga atggaatgga
     5341 tctgccgata ttaatgatcc agctgagtac ttcattaact cccttatggg gagcgatcga
     5401 tttaggcgcc gtaatcacaa ctaagattcc cagcggggtg cccaacaaat aagccagaac
     5461 cgtagaaatg aatgttatat ataaagtctg caacgttgcg tcgattaata attgatgaaa
     5521 ttcactcaac aagtttacaa ggtaagtaaa aaatgacatt gcgttattcc tcactcccaa
     5581 tcttgccgcg catcaactgc cgggtaattt ccatgctcgg gttcaacaaa acttgtccga
     5641 cttccccttg atccacaatt cgagtattat caataaccgc tactttgctg cataatttct
     5701 taactaactc aatttcatgt gtgataatga cgatcgtcac cttatactgt tgattaatgt
     5761 cccataatag gtccaagatc tgtgtggtgg tcagcgaatc caatgcggaa gtcggctcat
     5821 cgcacaaaag tattttcggg gtattcgcca tcgctcgcgc tattgaaacc cgctgccgct
     5881 gtcctccgga tagctgagac ggataattgt gagctttatc cagaatattc accaaatcca
     5941 tcagttcctt tacgcggtct tgaatcttcg atcgcggata gccggctatt tccagcggaa
     6001 aagcaatatt ttcttccaca gtccgttgca tcagaagatt cggagattga aaaatcatcc
     6061 cgatttcacg ccgtagtttg cgtaaatcag tatcattgag tcgaacaaat ttttgatcct
     6121 ccgctttcgg gtcgctgacg tcttttaacc ttctgaaaat acatttccct tcaatcagaa
     6181 tctctcccca tgtcggttct tccagcaaat tgatgcagcg gataagcgtg ctcttgcccg
     6241 ctccgctcat gccgataata ccgtaaatat cttcctgttc tacatgcaga gagacatctt
     6301 ccagtgcctg aaaaggacca tccttcattt caaattgttt actcaagtgg cggatctcaa
     6361 tcatgcttat gaaaccttat ttacttattt ttcaaaaaaa aggctctttt acgatgaaaa
     6421 gagcctcacg aatcagacat aattatgaag ataaagaaaa gactatcttt gatacgttaa
     6481 gctcaaccgc cccgtatggc ggccggccat caacattccg tacccgaaat agatcatttt
     6541 tatcataagg ctagtttagt agaaatcgaa ttaccggtca agggattcct acatttttat
     6601 gacatttaat catcgtctac ggtcaaagaa tgatctcttt aataatgtgt ttaattatca
     6661 gatgatcaaa tatcctatca cccgaaatcc tctcaagatt caaaaattca gagaacagtc
     6721 tgctttaact ttgataaaat tatattgaca ttttttacac ggaaacaaaa atttttttat
     6781 aggatatgaa ttccatctca aacgaattta aaaaggggta tcaaggcata tggacaagat
     6841 caatgaaaac gaaaaagaat accgattcgg tgattgggga ccgaaatata tcatcaaagg
     6901 gccgcggatg aacatgggaa ttattcgttt aaaaccggga caaaagttga ctccgcattt
     6961 tcataaagtg atggaggaaa atttttatgt cgaaaaagga aatgttcaat taaatgttga
     7021 cgggaccgtt tataacttaa taccggggga tgtcgttcgt tgtgagccga atgaagttca
     7081 cgcactcgcc aatgtaagtt ccgaagaagc ccgtgtaatc tttatgttag cgccttacat
     7141 ggaatcggat aaatttgagg ttgaagagac aaaataagct gccgtaagat tgtccgaatc
     7201 ggcatttttg cagaaatgaa atgaaacaat gataaaggga gaaactatag gggataacct
     7261 ttctaaaaag gtaattttca ggcaaaacat ctattgtctt ttacccgctt tttacgacaa
     7321 ttttacagat taaatccttt ctttcggttg tcccatgtta tgattgtttt tgtttcaaga
     7381 atcatatttc cgctccgatc agcgggaaca aatcgttttt agagaatttt caaacagctt
     7441 attgaagatg tattttttgc catagaaagg tgagttttca ttttttatcg gaaaaaattc
     7501 gcacgagaat atgctaaacg gaaataattt gattcacaca acgatcactg cccacagcgg
     7561 ttgtgatgat acaccgatga attcgctgga atccgtttta tcggggataa atgaccgagc
     7621 ggatattatc gaagtagatg tccgagccga taaaaacggc agattaattc tctctcacaa
     7681 tcttgatcaa gagagaatat atgccggaca tgcggaatta atccaagttt tggagatttt
     7741 ccaaacgaat aaaaatattt gcttgaattg cgacatcaaa gagacagaga ttattccttc
     7801 ggtacttgaa tcagccgaac aatatcagct ttccaaagac agaatttatc tttccggaag
     7861 tgttacgccg gaattattgc gccaacggac ggaaataatc gaaaaatgcg acatccaatg
     7921 gaatatcgaa gaagcgatga ttccgttggt tgaaaagaaa ctgattgcag aaaggaaaga
     7981 gtatttaaga gagacgctgc ataccaatcc atgggaagtc ttacgtgacg aagtgcccga
     8041 tcccgataga taccttcccg atttgatcaa aatggcgaaa gatttttctc ttactacttt
     8101 caatatgcca ctcgagtatg ccacggatga ccgcattcga ttcttccata ataacgggtt
     8161 aaaagtctcg gtttggacag tgaataacag tgaagaaatc tacaggctgc tacgattaag
     8221 agtacgaaat atcaccacaa aaaaggtaaa aatggcaata aaggtcaaaa tggaatttct
     8281 cggttttacc
//
LOCUS       DAOMIV010000026         8217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8768_length_8217_cov_3.816222, whole genome shotgun sequence.
ACCESSION   DAOMIV010000026 DAOMIV010000000
VERSION     DAOMIV010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8768_length_8217_cov_3.816222"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="PKV59_01285"
     CDS             complement(<1..1522)
                     /locus_tag="PKV59_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HQP45414.1"
                     /translation="MNDKINYEPVSNDKAHLKNVAEWEASKVITPEIMETARKILLRV
                     IDGADVFKTIRANPVPKHGNISKDALVKAYREMVANGEIQEDPHLLAKIRMKPNRTLS
                     GVSTVTVLTRPFPCPGNCIFCPNDARMPKSYLPDEPGAARAFQNHFDPFAQVRSRLDT
                     YQALGHPTDKVELLILGGSWTSYPEDYQAWFVQRCLDAMNESDSESLLIAQQQNETAA
                     HRNVGLVVETRPDEITESNLKYLRKLGVTKIQIGIQSLDDEILNLNRRGHTVQQALEA
                     SKLCRAAGFKLVMHWMPNLLGSTPQSDREDFMRLWVEADQGLGFCPDELKIYPTQLVE
                     NSDLYEVWRAGKYLPYDTETLIDLIAGIKPFIPPYCRVNRIIRDIPSHHIVAGNTRSS
                     LRMDIHQRMAENGTRCNCIRCREIRDQAVDPMTLKLVDYSYYAAEAKEHFLQFVTPED
                     KIAGYLRLSLPTRSSPNLDIADLNEAAIIREIHVYGQELPVGMELKGAAQHAGLGKK"
     gene            complement(1512..2978)
                     /locus_tag="PKV59_01290"
     CDS             complement(1512..2978)
                     /locus_tag="PKV59_01290"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQP45415.1"
                     /translation="MKKATYFSYLLFLPFFLLTIVQNQAFANSVIEEKPAKIVYALAA
                     PFTTITDNISLPDFSDFWSGTSASQPETIPGKLLIVTSDPERFRTLFGEGDKNQIEFV
                     TDIHAAFSQSINNGNWLIVPFEQLDARFKVISIDEQNPLHKDFNDNLWPLTIRINGNE
                     NMTEQDWAAVQPLNRDPSKLTTMILTGVTALVRGTAAYMDVWGPEYPASNIGDTLREA
                     DILHVNNEVPFAKVCQQTESELARLVFCSKTSALELLKSIGTDVVELDGDHFQDYGDD
                     AVYYTLKLYKDANIPYYGGGANLKEAQKPLIVEDHGNKFAFIGCNGKEIGYAVASDTR
                     PGAAHCDIPLIVSQIKKLKASGILPIVTFQHIELYKIYPNEDMAADFKAVADAGAVIV
                     SGSQSHIPMAFDISKDSFIHYGLGNLFFDQAFFLPETSEATMDRHVFYNGKHISTEIL
                     TIKFTNNALNRFMTESERTAVLNRIFAESHIELDQDER"
     gene            3606..6215
                     /locus_tag="PKV59_01295"
     CDS             3606..6215
                     /locus_tag="PKV59_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45416.1"
                     /translation="MIPILLKVDEEPHPFYNMNVSKKWFWFWLVNAFEGIASVVMIFQ
                     TASEAESAVLFGLSPLRIGLVLGLLFLIGLCLRFAKNALTEQDASDSVKDNRRYQVVG
                     LVGLLSGYIVVLAWIFLRPPFGITAFSKSLFTRLIPALVWGGLFSVESIFFVWFFSSK
                     EDIKSRLNRNQKTLIWAVTCGILLIFLIVFALKTGLGLHIISGTFYRQGVSILEGHLI
                     FPLLILFPIIGLITFLQSRFLKKKAISEKGQKNFIRLTGLIIWLIASIVWIRTPFEGR
                     SYFLPALRPPNYNFYPSSDAEYYDMFAQSILIGDGFRNGMGVVRPFYAAFLALLHAIA
                     GNNYLSVTNLQIIVLALFPVAVYRIGTLLHFPLSGILAAWWVILREVYSIELTPVVQV
                     SNSRLMMSDLPTALIVSWLMVALIHWYQADEKRQSLFAIISGGLCGIAVQIRTQSAVF
                     IPVVLLFLLIRYSIEKRLNLHAIKSFGIFAVSVLIIILPWNIWNQIHPNSTAGESNSE
                     VNYLYSLYAHAANADQSGEDLDTGISHNVIELILNHPIPILQSFTAHFINNELSSLLI
                     LPVRTEPVTDSQQWFHDRSVFWYRESSENVLKQNKLSLFVYIIILSIGFGSAIRRTSW
                     AGAIPFLLHLTYSLGTAFAMTSGFRFILPVDWVTLIYFAIGCVAVMRFPFIYIWEMTD
                     QPAFTQITITDGFPKRSLSICLSFIIILLGLALPLCDSVIPRRFHSSSSSEEILNRWI
                     EMTPENKNLAIQNRLSQKIQNGQLEILEGRAVYPRIYTAHTGDSGGNSSIKVNADYDR
                     LVWMLLNGKVSVISLPVVKEDFDQPIPDPQDLIVIGEKKEDYLSAQWVTAVISGKVQT
                     WEAASIDKKDSDE"
     gene            6215..7909
                     /locus_tag="PKV59_01300"
     CDS             6215..7909
                     /locus_tag="PKV59_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45417.1"
                     /translation="MPDLKKSLSLHEPYILNAIAASWNLSASAESAEPIEEILARKII
                     QSIDSSSLRKDLPESVRPAIEKLVINNGKIQSGPFFRLFGEIEVMGEARFIREEPWKS
                     PQNPAQWLWYRGLIQKSFFDTPEGMREYVYLPDELYDKFRQELKNLPIEESNSPDLII
                     RPAAPREVVQIYAGNDSVLDLSCLILASLRTGHEFSLFYPVYGEKKTQFTVNLLQIAG
                     ILDQSFMPIAETGQSFLMIDRLSALLQLIRAWQQTEEMDELLWIKELAVDELFAHNPS
                     KLRTNVMQFLNRIHGNGWWSFSGFLSAVKQDSPDFLRNQNDSTAWLIRNREKNQYKEW
                     ENVEGIYLRYLLSGPLHWLGIVDIASNAPKSENPEKPNGFLPISAFRVSRIGERCLAE
                     SLGKEVPKDNYRKANLEKVPPKVTIDGRISVNPNVPRILRYHIARFCEWEKIQPDRWI
                     FRVTPESLLHAKENGLSVPSFIAMLKRSGEKALPEALLNAVEQWDRMDTQATMFQATV
                     LTATNPDWIASLLNSKDTSQWIQQQLNANTVFIKPKGEEAVRRALIEMGVLTDTRK"
     gene            7946..>8217
                     /locus_tag="PKV59_01305"
     CDS             7946..>8217
                     /locus_tag="PKV59_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HQP45418.1"
                     /translation="MSEETIMQGKASWQLEKTDPKEAELLKESLRNVHDPELGYSVID
                     LGLIRDVVKEENRAVITMILTTPFCPYGGHMIEQVRMAAMEALKIDV"
BASE COUNT         2240 a         1668 c         1879 g         2430 t
ORIGIN      
        1 gttttttccc taatccggca tgttgtgccg cacctttcaa ctccattcca accggtaatt
       61 cctgtccgta aacatggatt tcacggatga ttgcggcttc gttcagatcg gcaatatcca
      121 aatttggtga agaccgggta ggaagtgaaa gacgcagata tccggcaatc ttatcctcag
      181 gtgtcacaaa ttgcaaaaaa tgttctttcg cttctgcggc ataataagaa taatcgacta
      241 atttaagagt catcggatct actgcctggt ctctaatttc tcggcaacgg atgcagttgc
      301 atcgggttcc gttttcagcc attctctggt gaatatccat tcgcagactt gaacgggtat
      361 tccctgcgac aatatgatga gaagggatat cacggataat tcgatttacc cggcaatagg
      421 gaggaataaa gggcttaatt ccggcgatca aatcgatcaa cgtttctgta tcatacggaa
      481 gatattttcc cgcccgccat acctcatata aatccgaatt ctccaccagt tgtgtgggat
      541 aaattttcag ctcgtcggga caaaatccga gaccttggtc tgcttcaacc cataatctca
      601 taaaatcttc tcgatcggat tgaggggtac ttcccaaaag gttcggcatc caatgcatta
      661 ccagtttaaa gccggcagca cggcaaagtt tactcgcctc caatgcctgt tgaacggtat
      721 gacctcggcg gttcaaattg agaatttcat catcaagact ttgaatcccg atttgaattt
      781 ttgtcactcc caattttcgc agatatttta aattcgattc ggtaatttca tcgggtctgg
      841 tttcaacgac caatccgaca tttcggtgag ctgccgtttc attttgctgc tgagcgatca
      901 gaagactttc cgagtcgctt tcgttcatgg cgtctaaaca ccgttgaaca aaccaagctt
      961 ggtaatcttc gggataactg gtccaagaac cgcctaagat taataattcg actttgtcgg
     1021 tcggatggcc aagcgcctga taggtgtcca accgggatcg gacctgtgcg aaaggatcga
     1081 aatggttctg aaatgccctg gcagctcccg gttcatccgg aagataactc ttgggcattc
     1141 ttgcgtcatt gggacaaaaa atgcagtttc ccggacacgg gaagggtcgg gttaaaacgg
     1201 tcacagtgga gacgccgctc agagtgcggt tcggtttcat tcggatttta gcgagtaagt
     1261 gcggatcttc ctgaatttct ccgtttgcca ccatttctcg ataggctttt accaaagcat
     1321 ctttggaaat atttccgtgt ttcggcacag gatttgctcg aatcgtcttg aaaacatccg
     1381 cgccgtcaat gacccgaagc aatattttcc gagcggtttc cataatttcc ggggttataa
     1441 cttttgatgc ttcccattcc gcgacatttt tcaaatgagc tttgtcattc gaaacgggtt
     1501 cgtaattaat cttatcgttc atcctgatct aattctatat gactttccgc aaaaatgcga
     1561 ttcaggactg cggttctttc ggattcggtc ataaaccgat ttaatgcgtt atttgtaaat
     1621 ttaatcgtta atatttccgt actaatatgt tttccgttat aaaaaacatg tctgtccatt
     1681 gtagcttcgc tggtttccgg aaggaaaaat gcctgatcaa agaataaatt tccgagtccg
     1741 taatggatga acgagtcttt tgaaatgtcg aaagccatcg gaatgtgaga ttgactgccg
     1801 ctgacgataa ccgcaccggc atcggcgacg gctttaaaat ccgcggccat gtcctcattg
     1861 gggtagattt tgtacagttc aatatgttga aaagtgacga tcggaagaat accggaggct
     1921 tttagctttt tgatttgaga aactattaag ggaatatcac aatgggcagc tcccggacga
     1981 gtatccgatg ctaccgcata gcctatttcc ttgccgttac agccgataaa agcaaactta
     2041 ttcccgtggt cctcaacaat taggggcttt tgtgcttctt ttagattggc tcctccgcca
     2101 tagtagggaa tgtttgcatc tttgtataac tttaatgtat agtagactgc atcatcacca
     2161 taatcttgga aatgatctcc atccaattca acgacatctg ttccaattga tttcagcaat
     2221 tccaaggctg atgttttgct gcagaaaacc agccttgcca attcggattc ggtttgctga
     2281 caaacttttg cgaacggaac ttcattgttg acatgaagga tatctgcttc gcgcagcgta
     2341 tcgccgatat tcgaagccgg atattcggga ccccagacat ccatataagc agcggtaccg
     2401 cgaaccaatg cggtgacacc ggttaaaatc attgtcgtga gtttcgatgg atcgcgattg
     2461 agcggttgaa cagctgccca atcttgttcg gtcatattct cattcccgtt gattcgaatc
     2521 gtcagcggcc ataaattgtc gttaaaatct ttatgaaggg gattctgctc atcaattgat
     2581 atgactttga atctcgcatc cagctgttcg aaagggacga taagccaatt cccgttattt
     2641 atcgattgag agaaagcggc atggatatcc gtgacgaatt cgatctgatt tttatcccct
     2701 tcgccgaaaa gtgttctaaa tctttcgggg tcggatgtga cgatcagaag ttttccaggt
     2761 attgtttccg gttgggaagc cgaagtccca gaccagaaat ccgagaaatc cggcaatgag
     2821 atattatcgg taattgtggt aaacggagct gccagagcat aaacgatttt tgccggtttt
     2881 tcctcgatta ccgagtttgc gaaggcttga ttttggacga ttgttaatag aaaaaaagga
     2941 aggaacaata agtaagaaaa ataggttgct tttttcatcc cgctaattat aagaccgttc
     3001 aagtttcact catcaatttt tgaagaggaa gttgacttat ggcaagccgg acggggaggc
     3061 aggcgagagg gcttccctct cgaggtcctt ttaaaaaaac aatttggatt gcggtcgagg
     3121 agagtctgtg aaaagggcga tattgaatca ctcatcaatt ttcgaaaaag aagttgactt
     3181 tttgcaagcc ggacggggag gtaggcgaga gggcttccct ctcgaggtcc tttgaaaaaa
     3241 acaatttgga ttgcggtcga gaatggtctt tgaaaaaggc gatattgaat cactcatcaa
     3301 ttttcgaaga ggaagttgac ttatggcaag ccggacgggg aggtaggcga gagggcttcc
     3361 ctctcgaggt ccttttaaaa aaacaattcg gattgcggtc aaggatgatc tgtaaataga
     3421 actatattga atcgctcatc aattttcgaa gaggaatttg acttatgaca agccggagac
     3481 ggggaggcag gcgagagaaa agatttcaca tgttttttga gaatcatttc aatcggataa
     3541 aaatccgttt cagccgttta agacttctta aatatctctc aaatttttct ttataaaccg
     3601 gagagatgat tcccattttg ctcaaggttg atgaagaacc tcacccattt tataatatga
     3661 atgtgtcaaa aaaatggttc tggttctggc tggtaaacgc atttgaggga atagcatccg
     3721 tcgtgatgat atttcaaacc gcatccgaag cggaaagtgc agttctgttc ggtctgtcgc
     3781 cgctcagaat cggactggtt ttgggattgc tttttcttat cggtctttgc ctgcgattcg
     3841 ccaagaatgc tttgaccgag caagatgcct cagatagcgt aaaagataac cgcagatatc
     3901 aggttgtcgg tttggttggt ttactctcag gatatatcgt agttttagca tggatatttc
     3961 tgcgacctcc tttcggaatc acggcttttt cgaaatcttt atttacaagg ttaattccgg
     4021 ctttagtttg gggcggtttg ttttctgtcg aatcgatttt ctttgtatgg tttttttctt
     4081 cgaaagaaga tattaagagc aggctgaatc ggaatcaaaa aacgttgata tgggcagtaa
     4141 cttgcggaat tctgctcatt tttctgattg tgtttgcttt aaaaaccgga ttgggactcc
     4201 atatcatttc aggaacattt tatcgacagg gagtctcaat cctggaaggg catttgattt
     4261 tccctttgtt gattcttttc ccgataatcg gattgatcac ttttttgcaa agtcgattct
     4321 tgaaaaaaaa ggcgatatcc gaaaaaggcc aaaaaaactt tatccgattg acaggattga
     4381 tcatctggct gatcgcttcc attgtctgga tcaggacacc ttttgaagga agaagttatt
     4441 ttttaccggc gttaagaccc ccgaattata atttttatcc ctcttccgat gcggaatatt
     4501 atgatatgtt cgcacagagt attctgatcg gtgatggatt tcggaatgga atgggtgtgg
     4561 tgcggccgtt ttatgccgcg ttcttagcac tcttgcacgc cattgcgggt aataactacc
     4621 tttctgtgac gaatttacag ataatcgtat tagctttatt ccctgtagcg gtttatcgga
     4681 tcggtacttt gcttcatttt ccgttaagcg gcatcctggc ggcttggtgg gtcatcctgc
     4741 gggaagttta cagcattgaa ctgacccctg ttgtacaagt ttcgaacagc cgtctgatga
     4801 tgtctgatct tccgacagca ttaattgtga gttggctgat ggttgcgttg attcattggt
     4861 accaggcaga tgaaaaacga caatctctgt ttgcaatcat ttcgggcggc ttatgcggaa
     4921 ttgccgtcca aatcagaaca caatccgcgg tttttattcc ggttgtattg ctctttctcc
     4981 tgattcgtta ttcgattgaa aagcgcttga atttgcacgc cattaaatca ttcggaatat
     5041 ttgcggtttc ggtattaatc attattttgc cgtggaacat ttggaatcaa attcacccga
     5101 attcgacagc cggcgaaagc aactccgaag taaattatct ttattcactt tacgctcatg
     5161 ctgcgaacgc agatcagtca ggcgaagatt tggatactgg aatttctcat aatgtcatcg
     5221 aattaatcct aaaccatcca attcctattc ttcagtcttt tactgctcat tttataaata
     5281 acgaactttc ttcattattg attcttccgg tcagaacgga acccgtaacc gattctcaac
     5341 aatggtttca tgatcgatct gttttctggt atcgggaaag ttcggaaaat gttctcaagc
     5401 agaataagtt atcgcttttt gtttatatta ttatcctcag catcggattt ggaagtgcga
     5461 tcagacgtac ttcttgggcc ggtgccattc cgtttctgct tcatctcact tattctttag
     5521 gaaccgcttt cgcaatgacc tcaggttttc gttttatttt accggtagac tgggtgacac
     5581 tcatttattt cgcgatcggg tgcgttgcgg tgatgcgatt tcctttcatt tatatttggg
     5641 aaatgacgga tcagcctgct tttacgcaga ttacgataac ggatgggttt ccgaaacggt
     5701 cgctgtcaat atgcctttct tttataatta ttctgttggg gttggctttg ccgctctgtg
     5761 attcggttat tccaaggcgg tttcattcgt catcgtcatc cgaggaaata ttgaatcgat
     5821 ggatcgaaat gactcccgaa aataaaaacc ttgccatcca aaaccgatta tcgcaaaaga
     5881 tccaaaacgg acagcttgag attcttgagg gacgagctgt ttatccgcgt atttataccg
     5941 cccataccgg tgactcgggg gggaattcga gtataaaagt gaatgccgat tatgatcgtc
     6001 tggtctggat gttacttaat gggaaagtat cggtaatttc tcttccggtt gtaaaagaag
     6061 attttgatca gccgatcccg gatccgcaag atttaattgt aatcggggag aaaaaagagg
     6121 attatttatc cgcacaatgg gtcacggcgg taatttccgg caaggtccaa acgtgggaag
     6181 cggcgtcgat tgacaaaaag gatagcgatg aataatgcct gatctgaaaa aaagtctatc
     6241 tcttcacgaa ccttatattc taaatgcgat tgctgccagt tggaatctat ccgcgtctgc
     6301 cgagtcagcg gaaccaatcg aggaaatcct tgcgcgaaaa attattcaat caatcgattc
     6361 aagctcttta aggaaagatc tcccggagtc cgtcagacct gcgattgaaa aattagtaat
     6421 aaacaacggc aagattcaat cgggaccttt ttttcggtta ttcggcgaga tagaagtaat
     6481 gggtgaagca cgatttattc gtgaagaacc atggaaatct ccgcaaaatc cggcgcagtg
     6541 gctttggtat cgaggattga tacaaaaaag cttttttgat accccggaag gaatgagaga
     6601 atatgtctat ttaccggatg agctttatga caaattccgc caagaactga aaaacctgcc
     6661 tattgaagaa tcaaattcac ctgatttaat tattcgtccg gctgctccgc gggaagttgt
     6721 tcaaatttat gcgggaaatg attccgtctt ggatctcagt tgtttgatct tagcttcatt
     6781 gcgtaccggg catgagtttt cgttgtttta tccggtttac ggggagaaaa aaactcaatt
     6841 tacggttaat ctactgcaaa tcgccggtat attggaccaa tcttttatgc cgatagcgga
     6901 aacaggtcaa tcttttttaa tgatagatcg acttagtgct ttgcttcaac tcatccgagc
     6961 ctggcagcag accgaggaaa tggatgaatt gctatggatt aaggaattgg ctgtcgatga
     7021 attatttgct cacaatccga gtaaactacg aacaaatgta atgcaatttc ttaatcggat
     7081 tcatggcaac ggttggtgga gcttttcggg ttttctttcc gcagtaaaac aggattcgcc
     7141 tgattttttg cgaaatcaaa atgattccac ggcgtggttg attcgtaatc gcgagaaaaa
     7201 tcaatataaa gaatgggaaa atgtcgaagg aatctacctc cgatatttgc tttcgggacc
     7261 gcttcattgg ttgggaattg tggatatagc ttcaaatgcc cccaaaagtg aaaatccgga
     7321 aaagccgaat ggttttctac caatcagtgc ttttagggtc agcaggatcg gtgaaagatg
     7381 tttggctgaa tccttgggga aggaagtccc aaaagacaat taccgaaaag cgaatcttga
     7441 aaaagtaccg cccaaagtca ccatagacgg gagaatttcg gtaaatccga atgtaccgcg
     7501 gatattgcgt taccatatcg caagattctg cgaatgggaa aaaattcaac cggatcgttg
     7561 gatcttccga gttacccccg aatcattgct tcatgctaaa gaaaacggat tatccgtacc
     7621 ttcatttatc gctatgttga aaaggtccgg tgaaaaagcc ttgccggaag ccttgttgaa
     7681 tgccgttgag caatgggatc gaatggatac acaagcgacc atgttccaag cgactgtatt
     7741 aaccgcaacc aatcctgatt ggatagccag cttattaaat tcaaaagaca cttcacaatg
     7801 gattcaacaa cagctgaatg ccaataccgt attcataaaa ccgaaagggg aagaagctgt
     7861 tcgccgggca ttaattgaga tgggggtgct gacagatacc cgaaaatgac cgatgaggaa
     7921 aaaagcacaa gaaagggaaa tcaatatgag tgaagaaaca atcatgcaag gaaaagcttc
     7981 ctggcagttg gaaaaaacgg atccaaagga agcggaatta ttgaaagaat cattaaggaa
     8041 tgttcacgac cccgaattgg gatacagtgt gattgatttg ggattaatcc gtgatgtcgt
     8101 taaagaagaa aaccgtgctg tcatcacaat gattttaacg actcctttct gcccttacgg
     8161 aggacatatg atcgaacaag tgcgaatggc agcaatggag gctttgaaaa tagatgt
//
LOCUS       DAOMIV010000027         8200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8791_length_8200_cov_4.533333, whole genome shotgun sequence.
ACCESSION   DAOMIV010000027 DAOMIV010000000
VERSION     DAOMIV010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8791_length_8200_cov_4.533333"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1629
                     /locus_tag="PKV59_01310"
     CDS             316..1629
                     /locus_tag="PKV59_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45419.1"
                     /translation="MSSDKEKIKQIFNIFWPLAISWLFMAVDIPFVSAIVSRMPQPEI
                     NLAAHSVVYPIALIIEAPIIMMLSVSLALSKDKKNYRRIYKFMMILCAGLTIIHASIS
                     CTPLFYLVVGNLMGAPKELLPYARVEMIMMIPWTWAIGYRRFYQGILIRNGHSKQVTI
                     GTIIRMSFLIGVLLIGYRLQDFVSGTMTAGAALSSGVIAEGLFCGLKGRKTADRDFEE
                     GELSELISWGELIRFVIPLILTQIINLGWMSLGSAAMSRMINPIASLAVWPVLSGLLN
                     IIKSFGSACNETTLSVINREGFYKADRRFTFYIAIFSTLFYAALIVTPLGRFWFEGLS
                     SLTPELSDLAKKALPMIFLVPFVNTYLNFYQAILLAGKKTSGFLESLLIYLIFMISIL
                     YIGILTQKWEGIYVIMIGMNIAVLAQTLWTRFRAKKIIPLFDHPK"
     gene            complement(2160..3137)
                     /locus_tag="PKV59_01315"
     CDS             complement(2160..3137)
                     /locus_tag="PKV59_01315"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HQP45420.1"
                     /translation="MSSLNSSNFFIGNIPVRGKTILAPMDGYSDSPFRQITRKLGSAA
                     SITEFVNTMEVVSRNTKYEERCSFQPSERPIGIQLFDNNPDRIIRGAEIIVNKWNPDW
                     IDINMGCSISHVANRGAGVGLLKDPHLVAEIISGLTGRLSVPITAKIRLGWDNESINF
                     LEIGKILQDNGAAMVTLHARTGKQLFSGNPNWDAIALLKQTLQIPVIGNGDIKSVSEI
                     KSLLDYTSCDAVMIGRAALQNPWIFAGYDADEVPLFLFQEICLEHLSLMKSFYGDTRG
                     CILFRKHAKKYLSHTGLEKHLIRDLLTTSDPDLFTEKFTDLIISTAQLI"
     gene            complement(3125..3811)
                     /locus_tag="PKV59_01320"
     CDS             complement(3125..3811)
                     /locus_tag="PKV59_01320"
                     /inference="COORDINATES: protein motif:HMM:NF022970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HQP45421.1"
                     /translation="MEPITIGNIVRVNHSFIDEDSAINLKGHEGVVRMIFSPEENDPD
                     ETLYLIEWTEKTLKQLPDDYYIKLFTDEKEWSYKIVPEHILLKVEGEYNADAVEWEKN
                     AIGARLFWQYFGRSGKLIQSLFPNDKEKTDRSPYDTWLKYLNQNLRFPFEATIFYEYE
                     PDDGILQPSDSVNVQGLAGWDYPLGIFCEVQFKGKYFILPLQDIGMTNELSKNARILN
                     AYEAWLLCRA"
     gene            complement(3910..4833)
                     /locus_tag="PKV59_01325"
     CDS             complement(3910..4833)
                     /locus_tag="PKV59_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQP45422.1"
                     /translation="MKKVLIATEKPFSKAAVEKIKSILESGDITVKLLEKYTDVNQLY
                     EAVADVNGLIVRSDEITKDVIDHAPNLEIIVRAGAGYDNIDLQAAKERGIVVENTPGQ
                     NSNAVAELVAGMMIMNARNFYDGSTGTELKGKKLGIQGFGAVGKCLARIANGLGMYVH
                     FYDIFVPFEAGYEYNVFPVEDIKYLYKTCQYVSLHIPALPSTIKSINYDLMSLMPQGA
                     VLINSARAEIIDEDDLLKMFETRPDFHYLADVAPSNKAVIEEKFPGRFFFTPKKCGAQ
                     TSEANNNAGTAAANQIVSFFLTGDKKYQVNK"
     gene            complement(4856..5926)
                     /gene="serC"
                     /locus_tag="PKV59_01330"
     CDS             complement(4856..5926)
                     /gene="serC"
                     /locus_tag="PKV59_01330"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQP45423.1"
                     /translation="MKKHNFNAGPAILPDYTYEKCIEAIKDFDNTGLSILSVSHRSKE
                     FDAVMDNAVAATKRLLDIPSGYSVIFLGGGASTQFYTVPYNFLGKKAAYLDTGTWAMG
                     GIKEAKLFGEVDIVASSKDKNYSYIPKNYTVPEDADYFEICSNNTIEGTEIREDFDCK
                     VPLVADMSSDIFSRPVDVSKYSLIFGGAQKNFAPAGVTIVIIKDSFMDKVVRPVPTMA
                     NYKVHVENNSMKNTPPVFPIFAGLKTMEWMEQSGGVKEMEKRAIARSSKIYDALDSSK
                     LFKATVEDPQDRSRMNICFVLKPEYSELETAFLDLAKQRGLVGIKGHRSVGGFRASCY
                     NALPMESVDALIDTIHEFEKAH"
     gene            complement(6088..7395)
                     /gene="lysA"
                     /locus_tag="PKV59_01335"
     CDS             complement(6088..7395)
                     /gene="lysA"
                     /locus_tag="PKV59_01335"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQP45424.1"
                     /translation="MTDSDLKRLYPISATINSKNHFTIAGSDLSDLAESYRTPFYIYD
                     RDTIHENIHRIQSAMADFYSGPGTIAYASKAYLSRKFASKIATEEVDLDVVSLTELRF
                     ALAAGFSPDRIHFHGNNKSIEEIEIAVDQNIHAIVVDNMDELLLTERIANSKKKVAQI
                     WLRITPDIRVATHPHIETSAATSKFGFHIINGDAANAIEYAVNSSALCLTGLHCHLGS
                     QLFDPLPYQIAIEKMFDLAINCHYIPLELSPGGGWGVPYVEDDPDSNPEQWVKAISET
                     ITKKCNRYQIRLPKLILESGRFIVARSGVAIYQIGTQKKSVNGERIIAVDGGMSDNPR
                     NALYQAKYTATPVDQSVHSRKNPCKVVGKYCESGDVLIDNVMLPDLNYGDLLMIPVAG
                     AYQLSMASNYNLAPRPVVLWLEPEGKVEVLQERENLEQNSWWD"
     gene            7568..>8200
                     /gene="rffA"
                     /locus_tag="PKV59_01340"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             7568..>8200
                     /gene="rffA"
                     /locus_tag="PKV59_01340"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HQP45425.1"
                     /translation="MISFNVPPYIGEEEKYIEKAIQNHKISGDGEFTHLCSSWLEEKT
                     GAKKVLLTTSCSDALEMAALLCDIREGDEIIMPSFTFVSTANAFVLRGAKIVFVDIRP
                     DTLNIDESLIENAITNRTKAIVPVHYAGIGCEMNTIVKIAEKYHLMIIEDAAQGVMSS
                     YEGKALGSIGDFGCYSFHETKNYSMGEGGAILIRDESFIEKAEIIREKGTN"
BASE COUNT         2442 a         1631 c         1582 g         2545 t
ORIGIN      
        1 catatctgcc aaagttgaat catttcgatt tcctgaaatt gaggaaaaac tgaacgaatt
       61 atccaacggt aagatcatcc ttgaaaaaat ttccgaaaga aaaacggaaa ttcgaccgat
      121 ttgatttttc ggtaaagaca ttttcctcag agcaatttat atcggattat tcaaataaaa
      181 tttgaactga tttaatagat tcatcatcaa atctattaat attttaacga atataaaaca
      241 taagaataaa aagatcaaaa aattggattc atggactcga agaaaaaacg gcatccgagg
      301 ataaaataga atggcatgtc aagcgataaa gaaaagataa aacaaatctt caatattttt
      361 tggccgttgg caatcagttg gctttttatg gctgtcgata ttccctttgt atcggcgatt
      421 gtcagtcgaa tgccgcagcc ggaaataaac ttagccgcac acagcgtagt ctatccgatc
      481 gcattaatta ttgaagcacc tattattatg atgctttcgg tttccttagc tctcagtaaa
      541 gacaaaaaga attatcgtcg tatatataaa tttatgatga ttctttgcgc gggattgacc
      601 attattcatg cttcaatcag ttgcacacct ttgttttatt tggtggttgg aaatttaatg
      661 ggagctccga aagaattatt accttatgcc cgtgtcgaaa tgatcatgat gattccgtgg
      721 acctgggcga tcggatacag acgcttttat cagggaatat tgattcggaa cggtcattcg
      781 aaacaagtaa cgattggaac catcattcga atgtcttttc taatcggggt cctgctgatc
      841 ggttaccggt tacaagattt tgtcagcgga acgatgacag caggtgcggc tttatcttcc
      901 ggcgtaatcg ctgaaggact tttttgcgga ttaaaaggaa gaaaaaccgc cgacagagat
      961 tttgaagaag gagaattatc cgagctgata tcttggggag aactgattcg atttgtaata
     1021 ccgttgattt tgacgcaaat aattaatctg ggttggatgt cgttaggtag tgcggcgatg
     1081 agtcgaatga ttaatccgat tgcttcatta gcggtttggc ctgttttatc cgggctttta
     1141 aatattatta aatcttttgg cagtgcatgt aatgagacga ccttatctgt tatcaatcgt
     1201 gaaggattct ataaagctga tcgtagattt acattttata tagcaatttt cagtacattg
     1261 ttttatgccg ctttaattgt tacaccttta ggtcgttttt ggtttgaagg tctttcttcc
     1321 ctcacaccgg aattatccga tcttgcgaaa aaggcccttc ctatgatctt tctggttccg
     1381 tttgtcaata cctatttgaa tttttatcag gcgattttac tcgccggaaa aaagaccagc
     1441 ggatttttgg agtccctgtt aatttattta atttttatga tatcgattct atatatcgga
     1501 attttgaccc aaaaatggga aggaatctat gtaatcatga tcggaatgaa tattgcggta
     1561 ctcgcgcaaa ctctttggac aagatttcgt gccaaaaaaa ttattccgct atttgatcac
     1621 ccaaaataag attcgattta aatttcgctg tccttaatca aatgatgaat taattgctct
     1681 tcaaaatcta tggaataatc agctcgaatt gcgttttgtt tgattttttg gatctcattt
     1741 tgactgagcc catcattaaa gggaattttt atgacggatt caattttgcg gattaattcg
     1801 gaatgcttat ccggattttg atataaagga tgccaacaag gaacgcttct tttttcgtat
     1861 agacatcgat atgcacaatg tcgaggcagc caattatgta tttcttgaat attatctgga
     1921 gatattttta tgcaagtcga cattttttga aaccgatcag gataaacctc acataatccg
     1981 gattcgagat ttaaataagg acatactact tcggtataaa aaatttccga agtgtcctca
     2041 tcttcaattt tataaaagca gcaaactccg caatgatcgc ataaaagttc ccattccgat
     2101 tcggtaaaaa gattttgaag acaattttcc atttttatat tttctttgaa ttgaaaaaac
     2161 taaataagtt gagcggtaga aataatcaaa tcggtaaact tttcagtaaa taagtcagga
     2221 tccgaagtag tcagtaaatc ccgaatcagg tgtttttcca aaccggtatg ggataaatat
     2281 tttttggcat gcttccgaaa cagaatgcaa ccccgagtat caccgtaaaa acttttcatc
     2341 aaagacaaat gttccaaaca aatttcttga aataagaaca aaggaacctc atccgcatca
     2401 taaccggcaa aaatccatgg attttgcaga gcggcacgtc cgatcataac agcatcgcaa
     2461 cttgtataat caagcagcga ttttatctcg gaaacggatt tgatatctcc gttaccgatg
     2521 accggaattt gaagtgtttg ttttaataaa gcaattgcat cccaattcgg atttcctgaa
     2581 aacaattgtt taccggtacg tgcgtgcaat gtaaccattg ccgcgccgtt atcttgtaag
     2641 attttaccga tctccaagaa gttaatgctt tcattatccc atcccaaacg aatttttgcc
     2701 gttatcggga ccgaaagtcg accggttaat cccgaaatta tttctgcaac caaatgggga
     2761 tcttttaata aaccgacacc ggcaccccgg ttagcaacat ggctgattga acagcccata
     2821 ttgatatcaa tccaatcggg attccactta ttaactatga tctccgcccc tcgaataatc
     2881 cggtcgggat tattgtcaaa aagctgaatt cctatcgggc gttcggacgg ttgaaaagaa
     2941 catctttctt catatttcgt attacgagag acaacctcca tcgtgttaac gaattccgta
     3001 atactggcgg cggatccaag tttcctggta atttgacgga agggactgtc gctatatcca
     3061 tccatgggag caagtattgt ttttccccga accggtatat tcccgataaa aaaatttgag
     3121 ctgttcaagc tcgacataaa agccatgctt catatgcgtt cagtattctg gcattttttg
     3181 acagttcatt ggtcattccg atatcctgca gcgggaggat aaaatatttc cccttaaact
     3241 gaacttcgca aaatatgccg agaggataat cccagcctgc caatccctga acatttaccg
     3301 aatcggaagg ttgtaaaatt ccgtcatccg gttcgtattc ataaaaaatc gtagcttcga
     3361 aagggaagcg caaattttga ttcagatact ttagccatgt atcataagga gaacggtctg
     3421 ttttttcctt gtcattcgga aacaacgatt gaattaattt accggatctc ccgaaatatt
     3481 gccaaaataa gcgcgctccg atggcatttt tctcccactc tacagcatcg gcattatatt
     3541 ccccttcaac ttttaaaaga atgtgttcag ggacgatctt atacgaccat tctttttcat
     3601 ctgtaaacag tttaatataa tagtcatccg ggagttgttt taatgttttt tccgtccatt
     3661 caattaaata tagggtttcg tcagggtcgt tttcttcagg tgaaaatatc attcgaacaa
     3721 ccccttcatg ccctttcaga ttaatggcgc tatcttcgtc gataaaactg tgattgaccc
     3781 tgactatgtt tccgatggta ataggttcca ttgaaaaatt cctttcatat cggaaacgat
     3841 tatactattc cgagaaaaaa acagcctgcg ttcacaaaaa aagcaggctg ttttttaatt
     3901 ttaaactacc tatttattta cttgatattt tttatctccg gttaagaaga agctgacgat
     3961 ctgatttgcg gcggctgtac cggcgttatt attcgcttca gatgtctggg caccacattt
     4021 tttcggagtg aagaagaatc taccagggaa tttttcttcg ataacagctt tatttgacgg
     4081 tgcgacatcg gcaagataat ggaaatcagg tctggtttca aacattttaa gtaaatcgtc
     4141 ttcatctata atttctgccc gtgcgctgtt gatcaaaaca gctccctgcg gcataagtga
     4201 cattaaatca taattgattg atttaatagt agagggaagg gcagggatat ggagagaaac
     4261 atattgacac gttttataaa ggtattttat atcttctacc gggaatacat tatattcata
     4321 gcctgcttcg aatgggacga aaatatcata aaaatggacg tacattccca atccattggc
     4381 tattcttgcc aaacattttc ctacggcacc gaagccttga attccgagtt ttttgccttt
     4441 caattctgtt ccggttgagc cgtcataaaa atttctggca ttcataatca tcatgcccgc
     4501 aacaagttcg gcaaccgcat tggagttttg tcccggagtg ttttcgacaa cgatacctct
     4561 ttccttcgct gcttgaagat caatattgtc gtaaccggca ccggcacgaa caatgatctc
     4621 caaattgggg gcatgatcaa taacatcttt ggtgatctca tcgcttcgga caatcagtcc
     4681 gtttacgtcc gcaaccgctt catataattg gtttacatct gtatatttct caagcaattt
     4741 caccgtaatg tcaccgcttt ccaaaatgct tttaattttt tcaacagctg ctttggaaaa
     4801 aggcttttct gtcgcaatca atactttctt catgagatgg ccctttcttg ttgaattaat
     4861 gtgctttttc aaattcgtgg attgtatcaa tcagagcatc gacactttcc atcggtaaag
     4921 cgttgtaaca ggatgcacgg aagcctccca cagaacgatg ccctttgata ccgactaaac
     4981 cgcgttgttt tgcaaggtca agaaatgcgg tttcaagttc actatattcc ggtttcagaa
     5041 caaagcaaat attcatccga gagcgatctt gtgggtcctc aacggtagct ttaaacaatt
     5101 tactggaatc caaagcatcg taaatcttag aagagcgagc aatcgcccgt ttttccattt
     5161 ccttgacacc gccgctttgt tccatccatt ccatcgtttt taatccggca aaaatcggga
     5221 agaccggagg agtatttttc atggaattgt tctcgacatg tactttataa tttgccatcg
     5281 tcggaaccgg tcgaacaact ttatccataa atgaatcttt gataattaca atcgtaacgc
     5341 cggcgggagc aaaattcttc tgtgcaccgc cgaaaatcag actgtatttt gaaacgtcta
     5401 caggtcgact aaagatatcg gatgacatat cggcgaccaa cgggactttg caatcaaaat
     5461 cctcacgaat ttcagtccct tcgatcgtat tattgctgca aatctcaaaa taatccgcat
     5521 cttcgggaac cgtataattt ttgggaatat aagaataatt tttatccttg gaagaagcta
     5581 cgatatccac ttcgccgaat aatttagctt ctttgattcc acccatcgcc caagtacccg
     5641 tatcaagata agcggctttt ttgcccaaga aattgtaagg aacagtataa aattgagtcg
     5701 aagccccgcc gcccaagaag attacggagt aacctgacgg aatatcaaga aggcgtttcg
     5761 tcgctgcaac tgcattgtcc attactgcgt caaattcttt tgaacggtga gaaacggaaa
     5821 gaatagaaag ccctgtatta tcaaaatcct ttattgcttc tatgcatttt tcataagtgt
     5881 aatcaggaag gattgcagga ccggcattaa aattatgttt tttcattatt tctccttata
     5941 tcaaatcaga aaattatgat tttatttatc gattcccgct ttacatctat ctgaaccgca
     6001 agaaagatgt cgggattttg tatactttta ttctaatcca gtgataaata attttggtaa
     6061 tgtaattgtc atacaattaa aattacatca atcccaccaa ctgttttgct caagattttc
     6121 tctttcttgg agaacttcga ctttcccttc tggttctaac cataaaacaa cgggacgggg
     6181 agccaaatta taatttgaag ccatacttaa ttgatatgca cccgctaccg gaatcattaa
     6241 taagtcgccg tagttaagat cagggagcat aacgttatca attaaaacat ccccgctttc
     6301 acaatatttt ccgacaactt tgcatggatt cttacgacta tgaaccgatt gatcaaccgg
     6361 ggtcgccgta tattttgcct ggtacagcgc atttctggga ttatcggaca tcccgccgtc
     6421 aaccgctatg atcctctcac cgtttacgga ttttttctga gttcctattt gatagatagc
     6481 tactcccgag cgggcgacaa tgaaacgccc cgattctaaa atcaatttcg gcaaacggat
     6541 ttgataacgg ttacattttt tcgtaattgt ttccgagatg gcttttaccc attgttccgg
     6601 attgctatcc ggatcgtctt caacatacgg aacaccccaa cctcctccgg ggctcaattc
     6661 aagcggaatg tagtgacaat ttatcgctaa atcgaacatt ttctctattg cgatttgata
     6721 gggaagcgga tcaaagagtt gagaccctaa atggcaatgc aaccccgtaa gacaaagagc
     6781 cgaagaattt acagcatatt cgatcgcgtt tgccgcatca ccatttatga tatgaaaccc
     6841 aaatttgctg gtcgcagcgg aagtttcgat atgaggatga gtggcaacgc ggatgtccgg
     6901 agtaatcctc agccaaattt gagcaacctt ttttttcgaa tttgcgatcc tttctgtcaa
     6961 cagcaattca tccatattat cgacaacaat cgcatggata ttttggtcaa cggcaatttc
     7021 tatctcttcg atagatttat tgttcccgtg aaaatgaatt cggtccggac tgaaacccgc
     7081 agccagtgca aatcgaagtt ccgtcagact tacaacatct aaatcgactt cctcggtcgc
     7141 tatttttgag gcaaattttc gtgataaata agctttggaa gcatacgcga tagttcccgg
     7201 accggaataa aaatcagcca ttgccgattg gattcgatgt atgttttcgt gaatggtatc
     7261 ccggtcataa atatagaaag gtgtacgata tgattcagcc agatcggaaa gatccgaacc
     7321 ggcgattgtg aaatgatttt ttgaattgat cgttgctgat attggatata aacgttttaa
     7381 atcggaatct gtcatgatct gcgatcgtcc ttggttcagt aattcgtttt taataaaaaa
     7441 acgttatttc tataaattat attcgagccc gggattattc ataaataaag ttttattcaa
     7501 caatttgata taaaattagg gagacgatta cctaatttat tacgaagaaa gaagaaaacg
     7561 gatatcgatg atttcattca atgttcctcc ttatatcggt gaagaagaga aatatattga
     7621 aaaagctatt caaaaccata aaatatcagg cgacggagaa tttacccatt tatgcagttc
     7681 ttggctggaa gaaaaaacag gagcaaaaaa agttctgtta actacttcct gttccgatgc
     7741 cctcgagatg gctgccttat tatgtgatat ccgggaagga gatgaaatca tcatgccttc
     7801 tttcactttt gtatcaaccg caaatgcctt tgtattacgg ggtgcaaaaa ttgtttttgt
     7861 ggatattcgt ccggacacat taaatatcga tgaatctctg attgaaaatg cgatcacaaa
     7921 tcgaacaaag gcgatcgttc cggttcatta tgcaggtatc ggttgtgaaa tgaacacgat
     7981 tgtaaaaatt gccgaaaagt atcatctgat gatcatcgag gatgccgcgc aaggcgtcat
     8041 gtcttcctac gaaggaaaag cattaggatc gatcggggac tttggatgct acagttttca
     8101 tgaaacaaag aattacagta tgggtgaagg cggagcgatc ttaatcaggg atgaatcttt
     8161 tatcgaaaaa gcggagatca ttcgagaaaa aggaacaaat
//
LOCUS       DAOMIV010000028         8169 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8837_length_8169_cov_4.093172, whole genome shotgun sequence.
ACCESSION   DAOMIV010000028 DAOMIV010000000
VERSION     DAOMIV010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8837_length_8169_cov_4.093172"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="PKV59_01345"
     CDS             <1..1375
                     /locus_tag="PKV59_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQP45426.1"
                     /translation="PVFQLDAIPTLRLESVEYEIMEGDTISKIATTFQVSKKSLIEAN
                     NLINPDIISVGQKITIPPQTQDIRDKGFLILPDAELVYGPPSVSFQPEDFLSDHPKSY
                     LSTYLEPTPTPLPKTQKDSSKESPVRFTPRTGMEILFQVSAENSINPKVLLALMEFQS
                     ASISNSKPNDHQKKSVIAYLGKSYESLSRQLNWAADTLNYGYYQWKNKRINQWILDDD
                     TVVAVNNNINAGTAAIQYLFSQIYGKDDWVYAVSEFGFAMTYRNLFGIDSQNYSWENT
                     PESFPDLQLPFSAGEIWSFTSGPHYGWSSGTPWSALDFAPPDAIACSQSNLWVTASAP
                     GKIVYSKGGLVIEDLDEDSNMQTGWSILYLHIEERDRIEAGRTVESGDKIGHASCEGG
                     IADGSHVHIARRYNGEWVPIDAKHPLILSGWEAFSTGSIYDGYLVKDDKIVEAWYYKT
                     EESEISY"
     gene            1386..3191
                     /locus_tag="PKV59_01350"
     CDS             1386..3191
                     /locus_tag="PKV59_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQP45427.1"
                     /translation="MIIMIILVGFPSFACTRSVSKPAELTEAAIRFEITKIGMETSVP
                     KNLPLAEVQKTYQPIPYRSSTPVPTMPSYPIPTETAIKADNNAKQVATEKSHITPFRS
                     ISTLMPTPRNTIPIEPINADITFTPVPSVKAYVPVEIQPLIPTLEITIIPTEVVLPDL
                     ESINTEKLLYVTQNGDTLQNIANRFGVTSDQISSTQPISANGFLNPNINLFINAPKDR
                     VYESAVKILPDEYIVFSKTATDYDLQNEINQAGGYLSYYSEETSDGLMSGSEIIYKVC
                     RDYSINPIVIIALLEYKSHWVYGFPRTPAEKDYPMGWIESTNKGLYKQVSWAATALSE
                     GYYGWRYGKISQIPFYKYPKPPYPIYFEPTLNAGSVAIQYLFSQLYVWDTVDAAIYSE
                     EGFFSTFTSMFGNVWENYSPDPNGLNANLRQIELTLPFSKTEKWAMTGGPHAAWTTGS
                     PYGALDFAPPSQYHGCISSDRWVLAAAPGIVVRTGKGIVIVDMDGDGLEETGWSLMYL
                     HIATNEKVPVGTVLKRGSRIGHPSCEGGSATGTHVHLARKYNGEWINATGPLPFVLSN
                     WTAYEGSEEYLGGMTRGNEIVQASTVGSSESIIRY"
     gene            3242..4810
                     /locus_tag="PKV59_01355"
     CDS             3242..4810
                     /locus_tag="PKV59_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQP45428.1"
                     /translation="MKVLFFRNKVISFSLLFVAAVVFLTACGTNKSATEQSQTTLTFP
                     LQLSPTPTELSSVSETLSGASKYYEKRPNYMPGEPVDYIVQPGDTVSALASRFNTTQS
                     EILKANPIIPAVTTTLPPGMPMKIPIYYRSQWGSPFRILPDSLFANGPAQIGFSARSF
                     VDRQPGWFKYFTAYTGEQNRRGGEIVDYIATTYSISPRLLLALLEYQTGALTQKHRPE
                     GINTNSVFGFTDVNSKQLTAQLNKLSNYLNNYYYEYREGRELEYQLLDGSLYRIDPWQ
                     NATTAALQHYFAMIDPPNEFFYAIGPNGFQATYIALFGEIEDHPDYIPGSLTQPKFTL
                     PFKEGDSWAFTGGPHPGWGDGAPNSAIDFAPPSSASGCVPSDEWVRVPAGGTIVRTDT
                     GVAVLDLDDDKDERTGWNIFFLHLLTSSIPPVETRLNTGDAIGHPSCDGGTSTGTHFH
                     IARKFNGEWISAGGIIPFEFDDWKVENGTSAYSGKLKRYSSTVIACECADYTSIIHNG
                     PPLVPTPTSTVKPK"
     gene            complement(4824..5870)
                     /gene="tsaD"
                     /locus_tag="PKV59_01360"
     CDS             complement(4824..5870)
                     /gene="tsaD"
                     /locus_tag="PKV59_01360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQP45429.1"
                     /translation="MTNNEQIRILGIETSCDETSAAVVEDGCRIISNIVASQIDIHKK
                     YGGVFPEVASRQHVLSISPVIEEAMQKAHMSYDDLDAIAVTRGPGLAGSLVVGMNAAK
                     GLSLTTGLPLIGVNHLEGHIYSAWLYGSEDKPPLTQPDFPAVALLVSGGHSEIILIQS
                     HLHYERLGGTLDDAAGEAFDKVARLIGLEYPGGPSIQKAAVFGDPNRFRFPKSKLEGT
                     WDFSFSGVKTAVLRKVRELQTEGMEELPVSDLAAGFQSAVTGILYEKTMDAARLYNAR
                     CIIIAGGVSANKALRELFENQSDFPLFIPKLSLCTDNAAMVGAAGYQRFIANKFDDLA
                     MDVFPTWPLSRVSL"
     gene            complement(5860..7140)
                     /locus_tag="PKV59_01365"
     CDS             complement(5860..7140)
                     /locus_tag="PKV59_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQP45430.1"
                     /translation="MKKDIQNLMKQKDVDAIIVSGGSEHNPPMTYLAKGEFFTKAIIV
                     LLKDREPVLFYRPMERDNAAKTGMKKICFDEFPTETYLREWDGDQAKADALELRDILN
                     RVGLRNGKIAFCGKTEFGTQYAMVQAFRELVPEFEILGSEGIDVINQARFTKDLEEIE
                     AIRKMGLTVTEVVRRVENFIKNDCRIKGNLLVGKDGEEITIGDIKRKINLWLSELGGE
                     NPEGTIFSVGKDSAVPHNSGDDGQTIETGKTIVFDFFPCEMGGGYFYDFTRTWCIGKA
                     SDNLLNVYKQVKVVHDAIINSIEVGKPLKELQKQTCEMFEKMGYKTVLNSPHTLEGYV
                     HSISHGLGLNIHERPFSGFSAPDSDRAEIGTVFTVEPGLYYPEAKEPFGIRIEDTVFI
                     DPNGKANIIAPYPYEFVLDVAPYSDKNAEEGHDK"
     gene            complement(7166..7570)
                     /locus_tag="PKV59_01370"
     CDS             complement(7166..7570)
                     /locus_tag="PKV59_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45431.1"
                     /translation="MDKLRTYIFLGLFFSLPSGYGLYWLVMNTLPYLKERWLFFFLMI
                     IFITGIMMPIYAALNKYFFTIRKIIPKTVVRESLVTGLLCDILLWFQIGRVLNSTIIF
                     LCVGGFILIEILLRTRDTVQYRSGFDDMKKDE"
     gene            7722..>8169
                     /locus_tag="PKV59_01375"
     CDS             7722..>8169
                     /locus_tag="PKV59_01375"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQP45432.1"
                     /translation="MLFTVDIGNTNITLGMFRGEDLIMRWRMATNRNSMPDEFGMQFW
                     FLFEHNGINPENIEGICISSVVPQITSKVEDACDYYFKRPILEVNYKLKLGMKIDIDH
                     PAELGNDRIADSVAVKALYGTPSCLIDFGTATTFNILNREGNYVGGA"
BASE COUNT         2356 a         1825 c         1691 g         2297 t
ORIGIN      
        1 tccggtattt caattggatg ccattccgac cttacgtctt gaatcggttg aatacgaaat
       61 tatggaagga gatacgattt ccaaaatcgc cacgactttc caagtctcta aaaaatcgct
      121 tatcgaagcc aacaatctga tcaatccgga tattatttcc gtcggacaaa aaatcacaat
      181 tcctccgcaa acgcaagata ttcgagataa aggatttctt attttaccgg atgccgaatt
      241 agtatacgga cctccttcgg tttcttttca accagaagat ttcctttctg atcatccgaa
      301 aagttatctg agtacctacc ttgaacctac acccactcct ttgccgaaaa cacaaaaaga
      361 ttcttcaaaa gaatctccgg tccgatttac gcccaggacg ggcatggaaa ttttatttca
      421 ggtatccgcg gaaaattcga tcaatccgaa agtgctgctt gcattaatgg aatttcaatc
      481 ggcttcgatt tccaattcca aacccaacga tcatcagaaa aaaagcgtca ttgcatatct
      541 cgggaaaagc tacgagagcc tttcaagaca attaaattgg gcagctgata cattaaatta
      601 cgggtactat caatggaaaa ataaacggat caatcaatgg atcttggatg acgacaccgt
      661 tgttgccgta aacaacaata taaatgccgg aaccgcagcc attcagtatc tgttcagtca
      721 aatatacgga aaggatgatt gggtctatgc agtttcggag ttcggttttg cgatgactta
      781 tcgaaatcta ttcggcatcg attcgcaaaa ttactcatgg gagaatactc cggaatcatt
      841 tccggatctg caattacctt tttcagccgg agaaatatgg tctttcacca gcggtcccca
      901 ttatggctgg tcttccggta ctccttggtc agcacttgat ttcgctccgc cggatgcgat
      961 cgcatgcagt caaagtaatc tttgggtaac cgcttcggca cccggaaaaa ttgtttattc
     1021 aaaaggcggt ttggtcattg aagatctaga cgaagattca aatatgcaaa ccggttggtc
     1081 gatcctgtat ctgcatattg aggaacgaga tcgaatcgaa gccggccgaa cagtagaatc
     1141 gggggacaaa atcggtcatg cttcctgcga aggaggcata gccgacggat ctcatgttca
     1201 tattgcgaga aggtataatg gggaatgggt cccgattgat gcaaaacatc ctttgatcct
     1261 aagcggttgg gaagcttttt caaccggttc aatatatgac ggttatctgg taaaagatga
     1321 caaaattgtt gaagcctggt attacaaaac cgaagagagc gaaatatcct actagtcttt
     1381 taataatgat cataatgatc attttagttg ggttcccttc gttcgcctgt acacgttcgg
     1441 tcagtaaacc cgctgaattg acagaagcgg caattcgttt tgaaatcaca aaaataggta
     1501 tggagacttc tgttccgaaa aatctaccct tggcggaagt tcaaaaaacc taccaaccga
     1561 ttccttatcg gagttcaacc cccgttccga caatgccgtc ttatccgata ccgaccgaga
     1621 cagccattaa agccgacaac aatgcgaaac aggtagcgac agaaaaaagt catataacac
     1681 ccttccgctc gatatctact ttgatgccca ctccgaggaa tacgataccg atcgaaccga
     1741 taaatgccga tatcactttt actcccgtcc catccgtaaa agcctatgtc cccgtcgaaa
     1801 ttcaaccgtt aattccgact ttagaaatca caataattcc taccgaagtt gtattgccgg
     1861 atctcgagtc gatcaatacg gagaaactgt tatatgtcac tcaaaatgga gatacgctgc
     1921 aaaatatcgc caatcgtttt ggggtaacgt cggaccagat ttcgagtact caaccgattt
     1981 cggcaaatgg ttttttaaat ccgaacatca acctctttat taacgctccg aaagaccgtg
     2041 tctatgaatc cgcagtgaaa attctgccgg atgaatatat cgtcttttcg aagacagcta
     2101 ccgattatga tctccaaaat gagataaacc aagcgggagg ttatttgtct tattattccg
     2161 aagagacttc agatggatta atgagcggaa gtgaaattat atataaagtt tgcagagatt
     2221 attcaatcaa tccgattgtc ataattgctt tgcttgaata taagagtcat tgggtatacg
     2281 gatttccgcg cacgcccgcc gaaaaagatt atccgatggg atggattgaa tcgacaaaca
     2341 aagggcttta taaacaggtc tcgtgggcgg caaccgcgct ttcggaggga tattacggtt
     2401 ggcgatacgg aaaaatcagc caaattcctt tttataaata tcccaaacct ccctatccga
     2461 tttattttga acccacatta aatgccggtt ccgttgcgat tcaatactta ttttctcagc
     2521 tttatgtttg ggatacggtc gatgcagcca tctatagcga ggagggcttt ttctcgactt
     2581 ttacttcgat gtttggaaac gtatgggaga attattcgcc tgatccaaat ggattgaatg
     2641 ccaatttacg tcaaatcgaa ttaacattac cattttctaa aacagaaaag tgggcaatga
     2701 ccggaggtcc gcatgccgct tggactaccg gttcacctta tggagcattg gattttgcac
     2761 ctccttctca ataccatggt tgtatctctt ccgatcgatg ggtacttgcc gctgctcccg
     2821 gaatcgtcgt tcgcaccggc aaaggaattg taattgtcga tatggatggg gacggattag
     2881 aggaaaccgg ttggtctctg atgtacttgc atatagcgac aaatgaaaaa gtaccggttg
     2941 gtacggtgct taaacgagga tcacgaattg gtcatccttc ctgtgaaggc ggatcggcca
     3001 ccggaaccca tgttcattta gcaaggaaat acaacggaga atggattaat gctacgggac
     3061 ctttgccttt tgttctgtcg aactggacag cttacgaagg atccgaagaa tatctcggcg
     3121 gaatgactcg cggaaacgaa attgttcaag cgagtacggt aggatcaagc gaatcgatta
     3181 ttcgatattg atattttctg aaaaaacgaa tctctttccc ttatatgtta aaatgcggta
     3241 catgaaagtt ttatttttta gaaacaaagt gatttctttt tcactattgt ttgttgcagc
     3301 tgtcgttttc ttaaccgctt gcggcacaaa taagagtgct accgaacaaa gtcagacaac
     3361 tttgacattt cctttgcaac tcagtccgac accgaccgaa ttatcatcgg tatcggaaac
     3421 attgtcaggg gcttccaaat attatgaaaa acgaccaaat tatatgccag gagagccggt
     3481 tgattatatc gttcaaccgg gggatacggt ttcggcattg gcctctcgtt ttaatacaac
     3541 tcaatcggaa attttgaaag caaatccgat tataccggct gtcacgacca ctttaccacc
     3601 cggtatgccg atgaaaattc ctatctatta ccggagtcaa tggggaagtc cgttccgaat
     3661 tcttccggat tccctttttg caaatggtcc ggctcaaatc ggtttttctg ccagaagttt
     3721 cgttgaccgg cagcccggat ggtttaaata ttttacggct tataccggtg aacaaaaccg
     3781 acgcggcgga gagattgttg attatatagc gacgacttac agtatcagtc cgcgtttatt
     3841 attagcgctg ttggaatacc agaccggcgc tctcactcaa aagcatcgac cggaaggcat
     3901 caatactaac agcgtttttg gcttcaccga tgtaaattca aaacagttaa ccgctcaact
     3961 gaacaaactt tcaaattatt taaataatta ttactatgaa tatcgagaag gcagagagct
     4021 tgagtaccaa ttattggacg gatctttata tcgcattgat ccgtggcaaa atgcaacaac
     4081 ggcagctctc cagcattatt ttgccatgat tgatcctccg aatgaatttt tctatgcaat
     4141 cggtccgaat ggatttcaag ccacatatat cgctcttttc ggagaaatag aggatcatcc
     4201 ggattatatt cccggtagtt tgactcaacc gaagtttacg ctgccattca aagagggtga
     4261 cagctgggct tttaccggtg gtcctcatcc cggatggggt gacggagctc ccaattccgc
     4321 tatcgatttt gcccctcctt cttccgcaag cggatgcgtt ccgagcgatg aatgggtccg
     4381 cgttccggcg ggtggcacta tcgtacgaac cgatacgggt gtagctgtgc ttgatttgga
     4441 tgacgataaa gatgaacgga ccggctggaa tattttcttc cttcacttgc tcacttcttc
     4501 catcccaccg gtggaaacga ggctcaatac gggagatgcg atcggtcatc cgtcttgtga
     4561 cggcggaact tcaaccggaa cccattttca tattgcacgg aaatttaatg gtgagtggat
     4621 ttcagcggga ggaattattc cgtttgagtt tgatgattgg aaagtagaaa acgggaccag
     4681 tgcttattcg gggaaattaa aacgatattc cagcacggtt attgcctgcg aatgtgcgga
     4741 ttataccagc atcattcata acggtccgcc attggttccg actccgacct ctactgtcaa
     4801 gccaaaataa gacaaaaatt gatttacaga ctcactcgtg acaatggcca cgtcggaaaa
     4861 acatccatcg ctaaatcatc gaatttattt gcaataaacc gctgatagcc tgcggctccg
     4921 accatggcgg cgttatcggt acaaagagat aatttgggaa taaataatgg aaaatcggat
     4981 tgattttcaa acaactcccg cagcgcttta tttgccgaca cacccccggc tataatgata
     5041 cagcgagcgt tatataacct ggcggcgtcc attgtttttt catataagat tccggtaacc
     5101 gcggattgga aaccggcagc cagatccgaa accggcagct cttccatacc ctcagtttgc
     5161 agttctctga cttttctgag taccgctgtc ttaactccgc tgaaagagaa atcccatgtt
     5221 ccttcgagtt ttgatttcgg aaaacgaaat cgattcggat caccgaaaac cgccgctttt
     5281 tgtatggaag gtcctcccgg atattccaat ccgatcaggc gggctacttt atcgaaagcc
     5341 tctcccgcag catcatccaa ggtaccgccc agccgttcat aatgcaaatg agattgaatt
     5401 aatataattt ccgaatgtcc gccggagacg agaagagcaa cagccggaaa atcgggctgt
     5461 gtcagcgggg gtttatcttc cgaaccatat aaccaggccg aatagatatg gccttccaaa
     5521 tgattcacac cgatcagggg taatccggtc gttaaagaaa gtccctttgc ggcattcata
     5581 ccgacaacca acgatccggc caaaccgggt ccgcgggtca cagcaatcgc atcgagatca
     5641 tcataagaca tgtgcgcctt ttgcatcgcc tcttcaatga caggggatat cgaaagaaca
     5701 tgttggcgag aagccacttc aggaaaaact ccaccatatt ttttatggat atcgatctga
     5761 gacgcaacaa tatttgaaat aattcggcag ccgtcttcaa ccaccgccgc agaggtttca
     5821 tcgcaagaag tttcgattcc caaaattctg atttgttcgt tatttgtcat ggccttcctc
     5881 tgcattttta tcactatatg gtgcaacatc caagacaaat tcataaggat acggagcaat
     5941 aatgtttgct tttccgttgg gatcgataaa tacagtgtcc tcaatccgaa tcccaaacgg
     6001 ttccttcgct tcgggataat agagtcccgg ttctaccgta aaaacggtac caatctccgc
     6061 acggtcggaa tcgggagcgg aaaatccgct gaacggtctt tcatgaatat ttaatcccaa
     6121 gccgtgactg atggaatgca cgtagccttc caaagtatga gggctattca atacggtttt
     6181 ataacccatt ttttcaaaca tttcacaggt ttgtttttgt aattctttga gtggtttgcc
     6241 gacttctatg gaattaataa tcgcatcgtg aacaactttt acctgtttat aaacattgag
     6301 caaattatcg gaggctttgc cgatacacca tgttctggta aagtcataaa aatatcctcc
     6361 acccatttcg cacggaaaga agtcaaaaac aatggtcttc cctgtttcaa ttgtttggcc
     6421 gtcatctccc gaattatgcg gaacagccga atcttttccc accgaaaaaa ttgttccttc
     6481 cggattttct ccgccgagtt cgctcaacca cagattaatt tttcgtttaa tatccccgat
     6541 tgtaatttcc tcgccgtctt tgccgacaag cagatttcct ttgatccggc aatcattctt
     6601 aataaaattt tccacacgtc tgacaacttc ggttaccgtc aaacccatct tacggattgc
     6661 ttcaatttcc tcaagatctt ttgtgaatcg cgcctgatta ataacatcga ttccttcgga
     6721 accgagaatt tcaaattccg gaaccagctc tcggaatgct tgtaccatag catattgtgt
     6781 cccgaattcc gtttttccgc agaaggcaat tttcccgttg cgaagtccaa cccgattaag
     6841 aatatcgcgc aattccaatg catccgcttt tgcctgatcg ccatcccact ctcgtaaata
     6901 tgtttcggta ggaaattcat caaaacaaat cttcttcatg ccggttttcg cggcattatc
     6961 tctttccatc gggcgataaa ataagaccgg ttcccggtct tttaataaca caataatagc
     7021 tttggtaaaa aattcgcctt ttgctaaata agtcatcggt gggttatgtt cactgccgcc
     7081 tgaaacgata attgcatcaa catctttttg tttcattaaa ttttgaatat cttttttcat
     7141 tttttattcc cgaacgaaga tttctttact catctttttt catatcatca aagcctgacc
     7201 gatattgaac cgtatcccga gtccgcagaa gaatttcaat taagataaat cctccgacac
     7261 acaaaaaaat aattgtcgaa ttcagcactc gtccgatttg gaaccacaat aaaatatcac
     7321 aaagcaatcc ggtgactaag gattcacgaa ccacggtctt agggatgatt ttccggattg
     7381 tgaaaaaata tttattcagg gctgcataga tgggcatcat aattccggta ataaaaataa
     7441 tcatcagaaa aaagaacaac cacctctcct tcaggtaagg aagagtattc attaccaacc
     7501 aataaagacc atatccactt ggtaaagaaa aaaacagccc gagaaaaata tatgttcgaa
     7561 gtttgtccaa acggactcct tatttgccta tccgatcgaa tactattata atcgcattga
     7621 gtttcgtcaa agaccagccg tatatttcga aaacacatat aaaataaaag aatcgaacat
     7681 atttataata tttcttaaaa aacgataaat gatggagaaa aatgcttttt acagttgaca
     7741 tcgggaatac gaatatcaca ttgggtatgt ttcgtgggga agatttaatc atgcgttggc
     7801 gtatggcgac aaatcggaat tccatgccgg atgaatttgg gatgcaattt tggtttcttt
     7861 ttgaacataa cggcattaat ccggagaaca tcgaaggaat ttgtatttcc tcagttgttc
     7921 cgcagataac ttcgaaggta gaagatgctt gtgattatta ttttaaacgt ccgattctcg
     7981 aggtgaatta caaattaaaa ttgggtatga aaatcgatat tgatcatccg gctgagctgg
     8041 ggaatgatcg aatcgcggat tcagtggccg taaaagccct atacggaact ccgtcttgcc
     8101 taattgattt cggaacggct accacattta atatacttaa tcgggaaggg aattatgtag
     8161 gcggtgcta
//
LOCUS       DAOMIV010000029         8158 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8855_length_8158_cov_4.949648, whole genome shotgun sequence.
ACCESSION   DAOMIV010000029 DAOMIV010000000
VERSION     DAOMIV010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8855_length_8158_cov_4.949648"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..894)
                     /locus_tag="PKV59_01380"
     CDS             complement(55..894)
                     /locus_tag="PKV59_01380"
                     /inference="COORDINATES: protein motif:HMM:NF037296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45433.1"
                     /translation="MAIEFRPMRSIIYVDCCREEYRHRLQHWLYGHHIEDSISVFGPY
                     VTKYAFYNALPVPPEGERFGTRRMQMTEHYWLVNEMVPEMRNKAVSEYMPLNVLRWQG
                     CVPDTDETPDMNIDGDAGRSAGGDNGCQPFVFAHVPISWEEDFKGKGRMVIDGPNYRW
                     QFVIQYPSDLGFEEGENWFHNEFVPFFQSCPQVNRFLSSRIMQDVVGCPFHRLVEMWF
                     DGPEEWYQAAVTGTKDWKKPFWAQQDQFPFFKSQFNIVGMFLTDIPASDNLTQYRGYI
                     TMR"
     gene            complement(914..1849)
                     /locus_tag="PKV59_01385"
     CDS             complement(914..1849)
                     /locus_tag="PKV59_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQP45434.1"
                     /translation="MKILGISGGTPNGNNDAMCKQALMGAQEVGAEIEFINLNKLDIK
                     HCTGCKSCVMALFSGKGSLCSLKDDFNWLLDKIYDADGIVWSIPIFEKGSTGLFHTIT
                     DRFGPRMDRGNVIIGNKISSENGGVPIDPRFMEDKVVSYMGVGGSDWNTLIQCDFGMQ
                     ALTPMWKIIDNEIFPWCLDIIMNDAALERATQIGRNIAEAAKDYSKAEYKGIDGICPH
                     CHSRNFFLDEDGKAICCLCGIEGKIEVVDGKYKFTFPEEQLKHAHDTLSGKFLHGDDI
                     QRNEAKARAAMQTEEAKARQKKYREFITPLVPERK"
     gene            complement(2043..2945)
                     /locus_tag="PKV59_01390"
     CDS             complement(2043..2945)
                     /locus_tag="PKV59_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQP45435.1"
                     /translation="MTFFQLECFMVLAQRLNFTQAAAELFIAQPALSRIISSLERELK
                     VQLFERTPRSVTLTSAGKAFLSRCPAVLESYRNCVSAAHLAQKGLNGSLTLGILRDNF
                     EPCLPQLFKTFHNHFPQIALSLRGYSHSGLLAAFEFGEVDAILNYLQIPAAAENEELI
                     VLHRNRQCVITALDHPLAQRSSLRMAELKDEPFVVMTRTDSTPGHDFIWRIAAEAGFT
                     PQIVEEATHIPILITLVACGVGISTLTEDLKYLAQGKVAFIPLLGVPLSNLVFTWHSN
                     HTNPALPHLIQTVRSQAEGESCGS"
     gene            3111..4907
                     /locus_tag="PKV59_01395"
     CDS             3111..4907
                     /locus_tag="PKV59_01395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQP45436.1"
                     /translation="MGKDQFAAVELLWDILNGKTKEVSKVTYFPQKPDMPQDAKINQP
                     FPRATPESQGIPSERLSAMIEELSRYPWLDVHHVLVIRNGKMICECSFSPYRPNIWHS
                     SYSLCKSITGLAIGMLYDKGKIHLDTPITEFFKDKDRSRMSFRQKKITVENLLTMTSG
                     VDFHEAGAYTGDDWVKGYLGASVREMPGEIFDYNSMNSYMLSAIVTAVTGQSLMDFLT
                     PRLWKPLGITNVFWETCPMGITKGGWGLFICPEDAAKIGQLILQGGIWKRRRIISEEW
                     LKTAAEKHVEVPPESGLGYGYHMWMADRPGSSAFNGMLGQNVLIYPDINMVIVTNAGS
                     NELFQRSTLIATIEKYLGAGFEPVDPLPENESEYKKLLRIQSALSGEENDAFLQQLKG
                     WSGKILRRSNKPTPSRYVKKLDGMSYAFQKKNIGIFPLLLQVVHNNFTEGIQTLSFHY
                     QDEKFWISFLEGTEKIDLPVGFEEPARSTVKIHGEGYLVATGGEFAFNEDDILVLKLD
                     IAFLEEAVRRKIKIYFLEDSIEMKWDEVPGARLILKGVQYILTDVSDNFLIHTVSHRI
                     NSDLVIEMLRNAIQPVILGLPAEQEEPQQEQP"
     gene            5045..6676
                     /locus_tag="PKV59_01400"
     CDS             5045..6676
                     /locus_tag="PKV59_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PfaD family polyunsaturated fatty
                     acid/polyketide biosynthesis protein"
                     /protein_id="HQP45437.1"
                     /translation="MTNQSSFIDVSGMADHSLLSQKNMGEYIPLTPEKMKSYLSDLAK
                     PVFVIKNGKDAFLSDSVFLSSSANSWKALAYCFPFTPEMFGDADFKRSYGLKYALYGG
                     AMANAIASEEMVISLGNSGFMGSFGAGGMSLSAIEKAIYRIREALKDKPYAFNLINNP
                     FDSEMEQKTAELYIQNGIHVVEASAYLTINSSLVRYRAAGLKQLPDGSVEIGNKLIAK
                     VSRKEVASQFLQPAPEEILSRLLSEGKLTEEQVRLAKSVPMADDITVEADSGGHTDNR
                     PLVSILPAIIRLRDSFQAKYHFPHPVRIGAAGGISVPDATLAAFMMGAAYVTTGSVNQ
                     ACIESGASEYTKNLLAQAEITDVAMAPASDMFEMGVKVQVLKRGTMFAKRAQKLYDLY
                     QRYDSVEEIPAAEREKLEATVFKRTLDSVWEECQKFFSERDPKQLEKAAKDPKHKMAL
                     IFRWYLGLSSRWSSIGEKGREMDYQIWCGPSLGAFNDWVRGTYLEKPNNRRVADVSLH
                     LLTGAAYLYRIRLLESQGIRFSSDLRDYLPLQPLL"
     gene            complement(6680..7381)
                     /locus_tag="PKV59_01405"
     CDS             complement(6680..7381)
                     /locus_tag="PKV59_01405"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HQP45438.1"
                     /translation="MTIYLTFADFPPLESDFSAEYLSDFLGEQERIEWDRLKVPKRKR
                     EWLSARIIGKKLIRTSILELEQVPFSKIQILKEPGGAPFVSVENGKRTRIEISFSHSH
                     EHVFAACSPDNIRFGTDLEWIEPRSKEFVEDFFTENEIRRVVSSDPDQADLISTVIWS
                     AKEAVLKALRTGLRLDTRKIEIGFTTEPSDREGWLQLEIKSPSRLLWRREGDFVETFC
                     ILENPEQDLRWVTLR"
     gene            complement(7432..>8158)
                     /locus_tag="PKV59_01410"
     CDS             complement(7432..>8158)
                     /locus_tag="PKV59_01410"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HQP45439.1"
                     /translation="EKLEKIRFQAPLKFYGNKTRKIAWHAAAYRKPEGIVIKASLESD
                     IKRRDGTISHLNHFDSLVYLTQNPAEAESVTAAPKWGKRRSVSSEEIYKLYFHGPSFQ
                     VLDAAQLSESAVLGKFNKQLMNVPADESVPYMKSLLIELCFQSAGLWEVGSTGVLSLP
                     QSVGNLSFYKQPINKGPIFAEVQPREHEGQISFDAKVVDAKGNVILELEDYRTSPLPY
                     SADKKLVEPIKSMVTEQSKDSQH"
BASE COUNT         2189 a         1822 c         1952 g         2195 t
ORIGIN      
        1 ttggatgaaa aattcgcata ttaaaaacga ttgatcagag aacaggaatc tttttcaacg
       61 catggtgata taccctcgat actgtgtcag attgtcactg gcggggatat cggtcaggaa
      121 cataccgaca atattgaatt gtgacttaaa gaatggaaat tgatcttgtt gagcccaaaa
      181 cggctttttc caatctttgg tgccggtgac ggcagcctga taccattctt ccggtccgtc
      241 aaaccacatt tccaccaacc ggtggaaggg acaacccacg acatcctgca taatacggct
      301 ggagaggaaa cgattgactt gcgggcagct ttggaaaaat gggacgaatt cattatggaa
      361 ccaattttcg ccttcttcaa aaccgagatc gctcggatac tgaataacga attgccagcg
      421 ataattcggt ccgtcaatga ccatgcggcc ttttccttta aagtcttctt cccatgaaat
      481 gggaacatgc gcaaagacaa aaggctgaca accgttgtct cctccggctg atcgaccggc
      541 gtctccgtcg atattcatat cgggagtttc atccgtgtcc ggaacacatc cctgccaccg
      601 taatacattc aacggcatgt attcggaaac ggctttgttg cgcatttccg gcaccatttc
      661 attaaccagc caatagtgct cggtcatttg catccggcgt gttccgaatc gctctccctc
      721 gggagggacg ggtaaggcgt tataaaaagc gtattttgtt acataaggac caaagactga
      781 gatgctgtct tcaatatgat gaccatacag ccagtgctgc aatcgatgac gatattcttc
      841 tcggcagcaa tccacataaa tgatgctgcg catcggtcga aattctatag ccatggtaaa
      901 aaaaccttct ttcttatttt cgctcgggaa ccagcggggt gatgaattcc cgatatttct
      961 tttgtcgggc tttcgcttcc tcggtttgca tcgcggcacg ggctttcgct tcattccgct
     1021 gaatgtcgtc cccgtgaagg aatttgccgg ataaagtatc atgggcatgc ttcaactgtt
     1081 cttcgggaaa agtaaatttg tattttccgt caaccacttc gatcttacct tcaatcccac
     1141 aaaggcagca gatcgctttg ccgtcttcat ccaggaaaaa gtttcgactg tggcaatgag
     1201 gacaaattcc gtcgattcct ttatattctg ctttgctgta atctttcgca gcttcggcga
     1261 tatttcttcc gatttgtgta gcccgctcca gagcggcatc attcataatg atatccaagc
     1321 accacgggaa aatctcatta tcaatgatct tccacatcgg agtcagagct tgcatcccga
     1381 aatcacattg gatcaaagtg ttccaatcgc tgccgcccac tcccatgtac gagaccactt
     1441 tatcttccat aaaacgcgga tcgatcggaa ctccgccatt ttcggaggaa atcttatttc
     1501 cgatgatcac atttccgcga tccatccgcg gtccgaaacg atcggtaatc gtatgaaaca
     1561 gtccggttga gcctttttca aaaataggga tcgaccagac aattccgtcg gcgtcataaa
     1621 tcttatcaag cagccaattg aaatcatctt ttaagctgca aaggcttcct ttaccggaaa
     1681 ataaagccat gacgcaagac ttacaaccgg tacagtgctt aatgtccaac ttgttcagat
     1741 tgatgaactc gatttcggct ccgacttctt gcgctcccat taaagcttgt ttgcacatcg
     1801 catcattgtt gccgttcgga gttcctccag aaatacccaa tattttcata gtactccctc
     1861 cgggataaag atattatttg gtttgcaacg gattattgat tttgcttatc caaatatatc
     1921 ttattctgtt acgatgcttc tgtaaaatac cggaagtgaa tcacggctat acacttttgt
     1981 tatgatgaaa aaaggaatcg acttgataaa aggatcgaca agccgctggg ataggaaatc
     2041 atttaagatc cgcaggattc gccttcagcc tgtgatcgaa cggtctgtat cagatggggc
     2101 aatgccgggt tggtatgatt gctgtgccac gtaaagacca agttggaaag gggtactccc
     2161 aaaagcggaa tgaaagcgac cttcccctgt gccagatatt tcaggtcttc tgttaaggtg
     2221 gagataccga caccgcaggc aaccagcgta atcaaaatcg gtatatgcgt cgcttcttcg
     2281 acaatttgtg gagtgaaacc ggcttccgcc gcaattcgcc agataaaatc atggccggga
     2341 gtggaatcgg tacgcgtcat aacgacaaac ggttcgtctt tcaattccgc catccgtaaa
     2401 ctgctgcgtt gtgccaacgg atgatcgagg gcggtgatga cgcactggcg attccggtga
     2461 agaacaatca actcttcgtt ttctgctgcg gcagggattt gaagataatt caggatggcg
     2521 tccacttccc cgaattcaaa agcggccagc aaaccggaat gactgtagcc gcgcagcgac
     2581 agggcaatct gcggaaaatg attgtggaag gttttaaaaa gctgcggcaa acaaggttcg
     2641 aaattatcgc gcaagatccc gagtgtaagc gagccgttta accctttttg cgccaagtgc
     2701 gccgcggaaa cacagtttcg atagctttcc aaaaccgcgg gacaccggct gagaaatgct
     2761 ttgcctgccg acgtcagggt aacgctgcga ggtgtccgct caaaaagctg cactttcaat
     2821 tctctttcaa gcgaagaaat aatacgactg agggccggtt gggcgatgaa taattcggcg
     2881 gcggcttggg taaaattcag acgctgcgcg agcaccatga agcattccaa ttgaaaaaac
     2941 gtcatgagga tcctcttaaa atcgttatta aagaattata gcaaatccct ttaatcgatg
     3001 tcgtttttcg gcggatcgga ttgataccga aatgaaaagg gtcaattcga ttttaaataa
     3061 ttgaaggaat tttcgggctg cggtagaatg tgattgaaaa gaggttattc atggggaaag
     3121 atcagtttgc cgccgttgaa ttgctatggg atattctgaa cgggaaaaca aaagaagtca
     3181 gtaaagtgac atattttccg caaaagccgg atatgccgca ggatgcaaag atcaatcagc
     3241 cgtttccgag agcgacaccc gaatcccagg gaattccgtc ggaacgtctc tccgcaatga
     3301 tcgaagaatt atcgcgttat ccatggctgg atgtccatca tgtattggtg attcgaaacg
     3361 ggaaaatgat ttgcgagtgc agtttcagcc cgtaccgacc aaacatctgg cacagcagct
     3421 attcgttatg caaaagtatt accggtttgg cgatcgggat gttgtacgat aaggggaaga
     3481 ttcatttgga tactccgatt acggaattct ttaaagataa ggaccggagt cggatgtctt
     3541 ttcgtcagaa gaaaatcacc gttgaaaatc tgttgaccat gaccagcgga gtggacttcc
     3601 atgaagcggg agcttatacc ggagacgatt gggtgaaagg ctacttgggt gcgtcggttc
     3661 gggaaatgcc cggggagatt tttgattata acagcatgaa ttcctacatg ctgtccgcaa
     3721 tcgtaaccgc cgttaccggt caatctttga tggatttttt gacccctcgt ttatggaaac
     3781 cgctgggaat tacgaatgtt ttttgggaaa cctgtccgat gggaatcacg aaaggcggat
     3841 ggggcctttt tatttgtccg gaagatgcgg cgaagatcgg ccaactcatt ttacaagggg
     3901 ggatctggaa acggcgacgg atcatttcgg aagagtggct caaaacggct gccgagaaac
     3961 atgttgaagt accgccggaa agcggtttag gatacggtta tcatatgtgg atggcagatc
     4021 gtcccggaag ttcggctttc aacggaatgc tcggacagaa cgtgttgatt tatccggata
     4081 tcaatatggt tattgttaca aacgccggca gtaacgaatt gtttcagcgc agcacactca
     4141 tcgctacgat cgagaaatat ttgggagcgg gctttgagcc ggtcgatcca ctacccgaaa
     4201 acgaatccga atataagaag ctgttgcgaa ttcagagtgc gctttccgga gaggagaatg
     4261 atgctttcct tcagcaattg aaaggctgga gtggaaaaat cttacggcgg agcaataaac
     4321 cgaccccttc ccgctatgtt aaaaagttgg acgggatgag ctatgcgttt caaaagaaaa
     4381 atatcggcat ttttcctctg cttttacagg tggtacataa taattttacg gaaggaatcc
     4441 aaacgctttc atttcattat caggatgaaa agttttggat ttcctttttg gaagggacgg
     4501 agaagattga cctccccgtg gggtttgaag agccggcgag atccactgtc aaaattcacg
     4561 gagaaggata tttggtggct accggcggtg aatttgcttt taatgaagat gatattctgg
     4621 tccttaaatt ggacattgcc ttcttggaag aggcagtacg ccgaaaaata aagatttatt
     4681 ttttggaaga ttccattgaa atgaaatggg atgaagtacc cggagcgcga ttgattctca
     4741 agggagttca atatattctg acggatgtct ccgataattt tctgattcat acagtcagcc
     4801 atcggattaa ttcggatttg gtgatcgaaa tgctcagaaa tgccattcag ccggttattt
     4861 tgggattacc ggcggaacaa gaagagccgc aacaggaaca accgtaaacc cggcttttcc
     4921 aaagcggtct tttcgcagca ttcttttcgt catccttttg atacaattct cacgattcac
     4981 aaaaaaataa aaaaaccgcc tgaaccttaa gaaagtggct taaaatacaa aagttaggtg
     5041 aaaaatgaca aatcaatctt cctttattga cgtttcggga atggcggatc attctctttt
     5101 gagtcaaaag aatatggggg aatatatccc gttaactccc gaaaaaatga aaagttatct
     5161 ttcggatctg gcgaaacccg tttttgtgat caaaaacggg aaagatgctt ttctttccga
     5221 cagcgtgttc ctttcctcat cggcgaattc atggaaagcc ttagcttatt gttttccgtt
     5281 tacccctgaa atgtttgggg acgcggattt taaacgatcc tacggcttga agtatgctct
     5341 ttatggcgga gcgatggcta atgctattgc ttcggaagaa atggtgattt ccttgggaaa
     5401 ttcgggcttt atgggatctt tcggagcagg cggaatgagc ctctccgcaa tcgaaaaagc
     5461 catttatcgg attcgggaag ctctgaaaga taaaccctat gcctttaatt taattaacaa
     5521 tcctttcgat tccgagatgg aacaaaagac agcggaactc tacatccaaa acggtattca
     5581 tgttgtcgag gcttccgctt atctgactat taattccagc ctggtccgat atcgggctgc
     5641 gggattgaaa caattgccgg acggttccgt cgagatcggg aataaattga tcgcgaaagt
     5701 ctcacgaaag gaagttgcct ctcagttctt gcaaccggct ccggaagaaa ttctttcccg
     5761 cctgctttcc gaagggaaat tgaccgagga acaagttcgt cttgcaaaat cggttccgat
     5821 ggccgatgat ataacggttg aggcagattc gggggggcat acggataacc ggccgttggt
     5881 gagtattctg ccggcaatca tccgtctgcg tgattctttt caagcaaaat atcactttcc
     5941 gcatccggta cgcatcggtg ccgccggagg aatttcggtt cccgacgcga cgctggctgc
     6001 gtttatgatg ggtgccgcct atgttaccac tggttcggtg aatcaggcat gtatcgaatc
     6061 gggcgcttcc gaatatacga agaatttact cgcacaagcc gaaattacgg atgtcgccat
     6121 ggctccggca agtgatatgt tcgaaatggg cgtgaaggtt caggtgctga aacgggggac
     6181 gatgtttgcg aaaagagcgc aaaaacttta tgatctttac cagcgatatg attccgttga
     6241 agaaatcccc gcagccgaaa gagagaaact ggaagcaacc gttttcaaac gaactctgga
     6301 ctcggtttgg gaggaatgcc aaaaattctt ctccgaaaga gacccgaaac agcttgaaaa
     6361 agccgctaag gaccccaaac ataaaatggc attaatcttt cgctggtatt tgggtctttc
     6421 ttcccgttgg tcgagcatcg gcgaaaaggg acgagaaatg gattatcaga tttggtgcgg
     6481 accttcgttg ggcgctttca atgattgggt gcggggaacc tatctggaaa aaccaaataa
     6541 tcgcagagtt gcggacgtct cgctgcatct tttaaccggc gcggcatatc tttatcggat
     6601 ccgcttgctc gaatcacagg gcatccgctt ttcttccgat cttcgcgatt acttgccgct
     6661 tcaaccgctt ctctgaccgt tagcggaggg taacccagcg taaatcctgc tccgggtttt
     6721 ccagaataca gaacgtttcg acaaaatctc cttctctgcg ccataagagg cgggaaggag
     6781 attttatttc caactgcagc cagccttccc tgtcggacgg ttcggtcgtg aatccgattt
     6841 cgatttttct tgtatccagt ctcaatccgg ttctgagcgc tttcagcacc gcttccttgg
     6901 cgctccaaat gacggtggaa atgagatcgg cttgatccgg atcgcttgaa acaacccggc
     6961 ggatttcatt ttcggtaaaa aaatcttcga caaattcttt tgatctgggt tcaatccatt
     7021 ccaagtccgt gccgaaacga atattgtccg gagaacaagc cgcaaaaaca tgttcatgcg
     7081 aatgactgaa cgaaatttcg attcgagttc gtttcccgtt ctccactgaa acgaacggtg
     7141 cgccgccggg ttctttcaaa atttgaattt ttgaaaaagg aacttgttcc aattcgagaa
     7201 tggaagtacg aatcaatttt tttccgatta tccgagcgga tagccactct ctctttcgct
     7261 tgggaacttt cagacgatcc cattcgattc tttcctgttc tccgagaaaa tccgaaaggt
     7321 attctgcgga aaaatcagat tcaagaggcg gaaaatcggc aaaggtcaga taaatcgtca
     7381 tgaatagaaa caaatccccc gacctttatt atagccgggg gatttcgaga attaatgctg
     7441 agagtctttt gactgttctg ttaccattga tttgatcggt tcaacgagct ttttatccgc
     7501 ggaatagggt aagggagaag ttcgataatc ctccaattcg aggataacgt tcccttttgc
     7561 atcgacaacc tttgcatcaa aagaaatttg tccttcatgt tcccgcggtt ggacttccgc
     7621 aaaaatcgga cccttgttga tcggttgctt atagaaactt aagtttccga cggattgcgg
     7681 cagagaaaga acaccggttg agcctacttc ccataacccc gcagactgaa aacataattc
     7741 gatcaacaaa gatttcatat aagggaccga ctcatcggca gggacgttca tcaattgctt
     7801 attaaattta cccaatacgg cactttcgga aagctgagcc gcatccagaa cttggaagga
     7861 aggaccgtgg aagtacagtt tatagatttc ttccgatgaa acggatcttc gttttcccca
     7921 tttgggagct gcggtaacgg attcggcttc cgccggattt tgcgtcagat ataccagact
     7981 gtcaaaatgg ttcaaatggc tgattgtgcc gtcgcgacgt tttatatccg actctaaact
     8041 tgccttgatg acgatacctt ccggttttcg ataagcagcc gcgtgccagg cgatttttct
     8101 tgttttattt ccgtaaaatt taaggggtgc ttgaaagcgg atcttctcca gcttttcg
//
LOCUS       DAOMIV010000030         8119 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8910_length_8119_cov_4.528150, whole genome shotgun sequence.
ACCESSION   DAOMIV010000030 DAOMIV010000000
VERSION     DAOMIV010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8910_length_8119_cov_4.528150"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..132)
                     /locus_tag="PKV59_01415"
     tRNA            complement(61..132)
                     /locus_tag="PKV59_01415"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Arg,seq:ccg)
     gene            complement(164..823)
                     /locus_tag="PKV59_01420"
     CDS             complement(164..823)
                     /locus_tag="PKV59_01420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HQP45440.1"
                     /translation="MIRCRISYQKTGPLRYISHLDLQKVWIRVLLRAKIPLIYTQGFH
                     PAPKIATAWPLPLGWEGKNELIDLWFDWEDDLDTEDQKKRFIETLNAQSPLGLSINSA
                     EEVPAYSPALTKLIESADYRIKTAGRFLAEDLNNKIHHLLSQTSVERIRREKRYNLRD
                     LIESASVSEETDEGPVIHVRMIAKDSAMGRPDEFADALGLDSEYLSFCRDRFYLSIPE
                     H"
     gene            complement(820..1287)
                     /locus_tag="PKV59_01425"
     CDS             complement(820..1287)
                     /locus_tag="PKV59_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45441.1"
                     /translation="MLKKGLSTPGIKITWSDPDTTMFEAWCSRGDRRMGAVIHRAWQL
                     GAKFDAWQDHFNKEAWLQAFSENGLDPDFYVHRERSKNEVFPWDHIDIGVTKYTLRKE
                     FENGLEGVLRPDCRTGCYGCGINQAFRLVQPKAGEQKWFCPILNFSAERELTE"
     gene            complement(1333..2718)
                     /locus_tag="PKV59_01430"
     CDS             complement(1333..2718)
                     /locus_tag="PKV59_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HQP45442.1"
                     /translation="MKPQEIEKEIEKFLLKVQKPGRYVGGEFNSIYKDPSAIQTRVAL
                     AFPDIYDLGVPNLGIAIFYDLLNKLPNVWAERVYLPWEDMEKEMRERNIPLYTLESKT
                     PVNQMDILGITIPYESLYTNVLNLLDLSGLSVFAAERSEDDPLVIAGGHSTYNPEPMS
                     AFIDAFVIGEGEEVFPEIVTVYQLWKKQALPKSELLYRLSKVEGVYVPLFYQTTYNGD
                     GTVSAINPVNDSFPKRIKKRILPVLPPPPDHFVVPSIDVVQNRIAIEIMRGCSRGCRF
                     CQAGFVTRPVRERPAEQILESIDSALSSTGYEEIALLSLSSSDYTQIQTLVDALQNRY
                     GKRKLNISLPSLRIDTVSVNILEKLKGSRFSGFTLAPEAASEKMEKTINKPITEEQLL
                     KTAEMIYSHGWQTIKLYFMIGLPGETDEDVMAICDLCLKVIRIGRKYLGKRSQLNIGI
                     ATLVPKPHTPF"
     gene            2924..3919
                     /locus_tag="PKV59_01435"
     CDS             2924..3919
                     /locus_tag="PKV59_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP45443.1"
                     /translation="MGKTPTIYDVSELAGVSTATISRVLNNPEKVKPCTKEKVMMAIQ
                     ELGYRPLLEARLRTAHDFERICVCAPRFTENSFVERLRGITVALAGQDYELQVYSVAS
                     KLQMDRFIDSLELRGLNGLITLSIPFTDEQIKRIKECGIEMVMIEYSSPLTNCVVIDD
                     RMGGKLAAQHLIEKGRKKFGFAAELNKSDYSVYPISGRLEGYQSTLKSHGFELNPRYI
                     YDVKCDTDQTYLNFLKIFRSGEFPDAIFAAADVQALGILRAAKEVGLNIPQDVAVIGF
                     DDIDFADYVGLTTIRQNLDHSGKVAVDLLINRLKDPERLMQLVQLPVELVVRKTT"
     gene            3970..5286
                     /locus_tag="PKV59_01440"
     CDS             3970..5286
                     /locus_tag="PKV59_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45444.1"
                     /translation="MKKVFSLILAVTMLFGAAAMVSAQDKTYLERAYDGEFKGKIVTL
                     DGPFIDNDQVLFEASIKDFEEKTGIDIQYIGNKDFESTIQMRVEGGNAPDIADFPQPG
                     GMANLAKKGYIVDVRDFISEDWLKENYSQAWLDLATVPNEKGEMIMGGIWARNNAKSQ
                     VYYRPKVWEEAGYETPETWQEMVDLSKQMVADGETPWCLGIEGGAGTGWPATDWVENI
                     MLRTTSLENYKKWTTGELKFDSPEVRRALEIMSEIFFTDGFVYGGREAIATTNFGEAV
                     KPLFYDTNGCMMYSLGNFITAYFPEDAVPGVDYDVFYLPPIDEQYGRPFLGAGDVYSV
                     FKGHDRDEVKATLEFFTHGESLKVWLANGGAMFPGKDADPSWYANATDAKISQMINDA
                     TSFSFDGSDQMPAAVGSGTFWKGMVDLVTGTDIDTVVKNIDKGWEQ"
     gene            5422..6516
                     /locus_tag="PKV59_01445"
     CDS             5422..6516
                     /locus_tag="PKV59_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP45445.1"
                     /translation="MNKTQTFGKSPSKIVLFFKMILILAGIILIFTAGVRIITFLDTN
                     HLKIPSALFAIVWGVSTVVLFYTGANWIVEHFSPKWTARIQPYIFVGPAVIILIWALT
                     LPVFRTLISSLKDADAKNWVGLQNYKYAFTDDQMLIVFRNNLLWLILGTFFSVFLGLL
                     IAVLADRSKYEKVYKSIIFTPMAISFIGAGVIWKFIYNYKGQGVETGLLNAIVMSFGG
                     QPHAWLQIPFWNNILLIVIMIWLETGYCMVILSSAIKGIPEELLEAARIDGASETTIF
                     FKIIIPVIKGSLIAVTTTVAIFSLKTFDVVQSMTGGNYGTNVIANEFYNQRFLYYNIG
                     RASAIAIVLLILVVPVMIMNLRQFNERKVF"
     gene            6516..7643
                     /locus_tag="PKV59_01450"
     CDS             6516..7643
                     /locus_tag="PKV59_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP45446.1"
                     /translation="MAQKEKSYKSQKKTSSFIVNLVLILISAVFVVPIFGILITSFRQ
                     SERIFNSGWWTVFPHKEWVEIGEYKLDADVDLNGEIKFVSEDGQEISGDYSFWKNGVI
                     TKSGEKIQYYGAKRSRTLKIYERRWTGFETDLTLENYRNVLSGKKITYKGEDGKLLTR
                     NGSDLSNSFINSLTVTIPATIIPITIAIFAAYGFAWLNFPGRKILFTMVVALLVVPLQ
                     IAIVPVLQDFSRIHLNGTFLGIWLAHTGFGLPLAIYLLFNYISEIPRDILESAFIDGA
                     TNFSIFTKLILPLSVPAIASFAIFQFLWVWNDYLVALVVLGGGTNEVVTQTLANMVGT
                     KGQDWHLLTAGAFISMIIPMTVFFSLQKYFVRGMMAGSVKG"
     gene            7752..>8119
                     /locus_tag="PKV59_01455"
     CDS             7752..>8119
                     /locus_tag="PKV59_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQP45447.1"
                     /translation="MNINPLWYKNAIFYQIYPRSFYDSNGDGYGDFQGIIQKLDYVRN
                     LGTDCIWLMPTYKSPLKDDGYDISDFYGVFEPFGTLDDLKQLFNEIHQRGMRVITDLV
                     LNHCSDQHPWFQSARKDPNS"
BASE COUNT         2283 a         1631 c         1804 g         2401 t
ORIGIN      
        1 tcggcacatg atcctaagtc atgcctgtct gccaattcca gcactccccc aaaaaaagag
       61 cgcgtctact gagactcgaa ctcagacttc tacctccgga gggtaacgtg atatccattt
      121 cactatagac gccgagcgtg aattatttta ccaccgattt ggattaatgt tccggaatcg
      181 atagatagaa tcgatctctg cagaaagaaa gatactcgga atcaagtccc aatgcatccg
      241 caaattcatc cggccttccc attgctgaat cttttgcaat cattcttaca tgaatgaccg
      301 gcccctcatc agtttcttcc gagacagaag cggattctat taaatcccgt aagttgtacc
      361 gcttctcacg gcgaatccgt tcgacggaag tttgggataa aagatgatga atcttattgt
      421 tcaaatcttc ggcgagaaat cttcccgccg ttttaatccg ataatccgcg ctttcaatta
      481 attttgttaa tgcgggactg taagccggaa cttcttctgc cgaattaatg gataatccta
      541 agggtgattg ggcattaagt gtctcaataa atcgcttctt ttgatcttcc gtatcaagat
      601 catcttccca atcaaaccaa agatcaatta actcattttt cccttcccag cctaaaggga
      661 gcggccaagc tgtagctatt ttcggagccg gatgaaatcc ttgagtataa atcaatggaa
      721 ttttggctct taaaagaact cgaatccata ctttttgcaa atccaaatgg cttatataac
      781 gaagcgggcc ggttttttga tacgagattc gacaacggat cattcggtta attccctttc
      841 ggcggaaaaa ttgagtatcg gacagaacca tttttgttct ccggctttcg gttgaaccag
      901 acgaaacgct tgattaatcc cgcaaccgta acaaccggtc cggcaatcgg gtcgcaggac
      961 accttctaaa ccgttttcga attctttacg aagagtatat tttgtcacac cgatatcgat
     1021 atggtcccag gggaaaacct cattttttga tctttcccgg tgaacataaa aatccggatc
     1081 caacccgttt tcggaaaaag cttgcagcca agcttccttg ttaaaatggt cctgccacgc
     1141 atcaaatttt gcgcccaatt gccaggctcg atgaataacc gcgcccatcc gtcgatcgcc
     1201 ccgggaacac caagcttcaa acatcgttgt atccggatcc gaccaggtaa ttttaatgcc
     1261 gggagtggaa agaccctttt tcagcaaatc ctgctttctt gcaatatttt ccggctcatc
     1321 ggctttccac cattagaaag gtgtgtgagg tttcggaacc aatgtcgcaa ttcctatgtt
     1381 caattgtgag cgtttaccga gatactttcg tccgattcta atcactttta aacataaatc
     1441 acagattgcc atgacatctt catctgtttc tcccggtaag ccaatcatga aatacaactt
     1501 aattgtctgc catccgtggc tataaatcat ttcagcggtt ttaagcaatt gttcttccgt
     1561 aatcggtttg ttaatcgttt tttccatttt ttccgaagcc gcttccggcg cgagggtaaa
     1621 tccgctgaaa cgagatcctt ttaatttttc caaaatattt accgaaaccg tatcaattct
     1681 taaggaaggc agcgaaatat ttaactttct tttgccgtac ctattttgca gcgcatcaac
     1741 gagcgtttga atttgagtgt aatcggagga agaaagggaa agaagtgcga tctcctcata
     1801 gccggtggaa gacaatgcgg agtcaatgga ttccagaatt tgctcggccg gtctttcccg
     1861 taccgggcgc gtcacaaaac ccgcttggca aaatcggcat ccgcgactgc agcctcgcat
     1921 gatttcaatc gcaatgcggt tttggaccac gtcgatggat gggacgacaa aatgatccgg
     1981 cgggggcggt aaaacgggga gaatacgttt ttttattcgt ttcggaaagc tatcgttaac
     2041 cggattaatt gccgaaaccg ttccgtctcc gttataggtc gtttgataaa aaagcgggac
     2101 gtaaacgcct tccactttcg aaagccgata aagcaattcc gatttcggca atgcctgttt
     2161 tttccacagc tgatatactg tgacaatttc ggggaatact tcttctcctt ctccgatgac
     2221 gaatgcatcg ataaatgccg acatcggttc cgggttatag gttgaatgtc cgccggcgat
     2281 aaccaaagga tcgtcttcag aacgttcggc ggcgaaaacg gataaaccgg acaggtccaa
     2341 aagattcagg acgtttgtat aaagcgattc ataaggaatt gtgataccta aaatatccat
     2401 ctgatttaca ggcgttttac tttccagcgt atacaggggg atatttcttt cgcgcatctc
     2461 tttttccata tcttcccaag gaagataaac acgctcagcc cagacattcg gaagtttatt
     2521 taagagatca taaaaaattg cgataccgag attgggaact cccaagtcgt aaatatcggg
     2581 aaaggcaagc gcaacgcgcg tctgtattgc tgatggatct ttgtaaatcg aattaaattc
     2641 tccgccgaca tagcgaccgg gtttttggac ttttaataaa aatttttcga tttccttttc
     2701 aatttcttgc ggtttcaagt tttatattcc tttggggtaa gctctttttc tatcatgtgt
     2761 tgattttatc caatgataaa acaaaaatca ctaaattggc atgattttct tattaattta
     2821 ctaaattact ctaagaaaaa atttgactta atccaataaa tggattaaat taaaatgtaa
     2881 taagttacaa tgtaagcagt tacattgtaa cggaaaggga aatgtgggca agacaccgac
     2941 gatttatgat gtatcagaac ttgcaggggt cagtacggcc acgatttccc gtgtcttaaa
     3001 taatccggaa aaagttaaac cctgtaccaa agaaaaagtg atgatggcta ttcaagagct
     3061 gggataccgc ccgctgcttg aagcacgatt gcgtacggcg catgattttg agcgaatttg
     3121 cgtttgtgcc ccgcgcttta ccgagaattc atttgttgaa cgactacgcg gaataaccgt
     3181 cgcgcttgcc gggcaggatt atgaattgca ggtttattcg gttgcttcca aacttcagat
     3241 ggatcgattc attgattcct tggaattacg cggtctgaac ggcttgatta cgctttcgat
     3301 cccttttacc gatgaacaaa tcaaaagaat taaagagtgc ggcatagaga tggtgatgat
     3361 cgaatattca tcgcctttaa cgaattgtgt tgtcatcgat gatcgaatgg gcggaaaatt
     3421 ggcagctcaa catttgattg aaaaaggcag aaaaaaattc ggatttgccg ccgaactgaa
     3481 taaaagcgat tacagcgttt atcctatttc cgggcgtctg gaaggttatc aatcgacgtt
     3541 aaaaagtcat gggtttgaat taaacccccg atatatttat gatgtaaagt gtgatacgga
     3601 tcagacttat ctgaattttc tgaaaatatt ccgttcggga gaatttcccg atgcaatctt
     3661 tgccgctgca gacgttcagg ctttgggaat tttacgagct gccaaagaag tcggattaaa
     3721 tatccctcag gatgttgccg ttatcggttt tgacgacatc gactttgcgg attatgttgg
     3781 tctgacgacc attcgtcaaa atttggatca tagcggtaaa gtcgccgtag atttattgat
     3841 caaccgtttg aaggatccgg aaaggttgat gcagttggtt caacttccgg tcgaacttgt
     3901 cgttcgaaag acgacataat ttcataacgc ctcaaaaagg tgttaaccat tttattttca
     3961 ggaggagaaa tgaaaaaagt attttcttta attttggcag ttacaatgct gttcggagct
     4021 gccgcaatgg tctccgcaca ggataaaacc tatcttgagc gggcttatga tggcgaattt
     4081 aagggaaaaa ttgtgactct ggatggacca ttcattgata atgaccaagt attgtttgaa
     4141 gcatccataa aagatttcga agaaaaaacg gggattgata ttcaatatat cggaaataaa
     4201 gattttgaat caacaatcca aatgcgtgtc gaaggcggaa acgcccccga tatcgcggat
     4261 ttccctcaac cgggcggaat ggctaatctg gctaaaaaag gatatatcgt tgacgttcgc
     4321 gattttatat ccgaagactg gttgaaagag aattattcac aagcctggct ggatttagca
     4381 accgttccta atgaaaaagg cgaaatgatt atgggtggaa tttgggctcg taacaacgcc
     4441 aaatcacagg tgtattatcg cccgaaagtt tgggaagaag ccggttatga aactccggaa
     4501 acctggcagg aaatggtaga tctttccaaa caaatggttg ccgatggtga aactccgtgg
     4561 tgccttggaa tcgaaggcgg tgcggggacc ggttggccgg cgacagattg ggtcgaaaat
     4621 atcatgctgc ggaccacttc gctggaaaat tataagaagt ggacaaccgg cgaactgaag
     4681 tttgacagcc cggaggttcg ccgcgcactt gaaattatga gcgaaatctt ctttaccgat
     4741 ggatttgttt atggcggaag agaagcgatt gcgacaacaa atttcggtga agccgtcaaa
     4801 ccgctttttt atgataccaa cggttgtatg atgtattctc tgggaaactt tatcacagct
     4861 tatttccctg aagatgcggt accgggagtc gattatgatg tgttttatct tcctcccatt
     4921 gacgagcaat acggaagacc gttcttaggc gccggcgatg tatacagtgt tttcaaagga
     4981 catgatcgtg atgaagtcaa agctaccctt gaattcttta ctcacggcga atcattgaaa
     5041 gtttggcttg ccaacggcgg agcgatgttc cccggaaaag atgccgatcc ttcttggtat
     5101 gcaaacgcta ccgacgcgaa gatatcgcaa atgattaatg atgcgacctc tttcagtttt
     5161 gacggttcgg atcaaatgcc ggctgcggtc ggatccggaa cattttggaa aggtatggtt
     5221 gacttggtta ccgggacaga tattgacaca gttgtgaaaa atatcgataa gggctgggag
     5281 caataatacc gaacctgttg taagaatgag tgcctccgtg aaagcggagg cacccctgtt
     5341 tctgttcttt cttacttggt accgaacctg aaattcggat cattcttttt acccccatgg
     5401 tgtatagaaa aggagggtta aatgaataaa acacaaactt tcgggaaaag cccgagcaaa
     5461 attgtccttt tcttcaaaat gatcctgatt ttggcaggaa ttattctgat ttttactgcc
     5521 ggagtcagaa tcataacatt tttagacacg aaccatctta aaataccctc tgctttattt
     5581 gcaatcgttt ggggagtcag tacagttgtc ttattttata ccggagccaa ctggattgta
     5641 gaacattttt cgccgaaatg gacagcgagg attcaaccgt atatatttgt cggtccggca
     5701 gttattatat tgatttgggc tttaacattg ccggtatttc gaactttaat cagttcatta
     5761 aaagatgcgg atgcaaaaaa ttgggtagga ttgcagaatt ataaatatgc atttaccgat
     5821 gatcaaatgc tgatcgtatt tcggaataat ttgctttggc tgattttggg aacttttttc
     5881 agtgtatttt tagggttgct gatagcggtt ttggcggatc ggtccaagta tgaaaaagtt
     5941 tataaatcca tcattttcac tcccatggct atttcattca tcggagccgg cgtgatttgg
     6001 aaatttattt ataactacaa gggacaagga gtggaaacag ggttgctaaa cgctatcgtt
     6061 atgtcctttg gcggacaacc acatgcctgg ctgcaaattc cgttttggaa taatattcta
     6121 ctgattgtga tcatgatttg gttggaaacc ggatattgta tggtgatcct ttcatccgct
     6181 attaagggaa ttccggaaga gttgttggaa gcggcaagaa tagacggcgc atcggagacc
     6241 actatttttt tcaaaatcat cattccggtg attaaaggat cattgatcgc ggttaccaca
     6301 acggttgcta ttttcagtct gaagacattt gatgtcgtcc aatctatgac aggaggcaat
     6361 tacgggacca atgtaattgc caacgaattc tataatcaac gtttcttgta ctataacatc
     6421 ggacgtgcat cagccattgc gattgttctg ctaattctgg tcgttccggt tatgatcatg
     6481 aatctccggc agtttaatga aaggaaggtg ttctgatggc tcaaaaagaa aaaagttata
     6541 aaagtcagaa aaagacatct tcttttatcg ttaatttggt cctcatctta atttcggctg
     6601 tcttcgtagt tccgattttt gggatcttga tcacatcttt ccggcaatct gaaagaattt
     6661 tcaacagcgg ttggtggact gtttttccgc ataaagaatg ggtcgaaatc ggtgaatata
     6721 aacttgatgc cgatgtcgat ctgaacggtg aaatcaagtt tgtatcggaa gacggacagg
     6781 aaatttccgg tgattattct ttttggaaaa acggtgttat cacaaaaagc ggagagaaaa
     6841 tccaatacta cggcgccaaa cgaagcagga cactaaaaat ttatgaaagg cgttggaccg
     6901 gatttgaaac ggatttaact ttggaaaact atcgcaatgt attaagcgga aaaaagatca
     6961 cctataaggg cgaagatgga aaattactca cccgtaacgg atcggatctc agtaattctt
     7021 ttatcaattc attaaccgta acgataccgg cgacaattat tccgattacc attgcaatat
     7081 ttgccgccta cggatttgca tggctgaatt ttcccgggcg taagattctt tttacgatgg
     7141 ttgtagcgct gttggtggta ccgcttcaaa tcgctattgt tccggtgttg caagattttt
     7201 cccggatcca tttaaatggg actttcttgg gaatctggct ggcacataca gggtttgggt
     7261 tgccattggc aatttatctg ttatttaatt acatcagtga aatcccgagg gatattcttg
     7321 aatcggcatt tattgacgga gcaacgaatt tttcgatctt taccaaatta attctgccat
     7381 tatccgtccc ggcaatcgca tcttttgcta tctttcaatt cctttgggtt tggaatgact
     7441 acctggtcgc tttggtggtc ttaggcggcg gaacaaatga ggtcgttaca caaaccttgg
     7501 caaatatggt cggaaccaaa ggtcaagatt ggcatttact gacagccggt gcatttatct
     7561 cgatgattat tccgatgacc gtattcttct ccttacaaaa gtattttgta cgcgggatga
     7621 tggcgggatc ggtaaaggga taatcaaaga ttattggcat ccccgcatgg aagaaatatc
     7681 ctacggggat cgttttttga aatcaattgt acgtattttg tcagactctt atttcttact
     7741 aaggagtttt tgtgaatatc aatcctctat ggtataaaaa tgcgatattt tatcaaattt
     7801 atccccgatc tttttatgat tcaaacggag acggatacgg agattttcaa ggaattattc
     7861 aaaaactgga ttatgtgcgg aatttgggga cggattgtat ttggttaatg ccgacctata
     7921 aatccccgct gaaagatgac ggatatgaca tctccgattt ttatggagta tttgaaccgt
     7981 ttggaacatt ggacgactta aaacaactct ttaatgagat tcatcaaaga ggaatgagag
     8041 ttattaccga tttggtactc aatcattgct ccgatcagca tccttggttt caatccgcca
     8101 gaaaagatcc aaattcgaa
//
LOCUS       DAOMIV010000031         8096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_8940_length_8096_cov_4.299465, whole genome shotgun sequence.
ACCESSION   DAOMIV010000031 DAOMIV010000000
VERSION     DAOMIV010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8940_length_8096_cov_4.299465"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKV59_01460"
     CDS             <1..920
                     /locus_tag="PKV59_01460"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HQP45448.1"
                     /translation="PTLSLGSGSGSAACTNKFEFLSDVTFPDGTMTLPLTTFTKSWYV
                     QNTGTCEWTKYYSIVYLSGDNVGTAKEFPLFTSDKLLKNGESAIISVIITAPEKQGEY
                     STYWALKSPDGQIFKGGDSAEGYPLSSKFKVGSQYNFYENLAGAICSDDSGLFFCGSS
                     DRSSGRGVAYYSASPTVESNYAGQPSIVIGPPLKENGKTRVTFGPIRVPRGTWLRAAV
                     SCPPNAPGCDNFVRLFVQVEGSTEQRVTETQENNDGYVTEWNIQLSDYGYHDQSLTYT
                     FEVQANGGSDADDLIIIQMPRLTDVAPVK"
     gene            1068..2171
                     /locus_tag="PKV59_01465"
     CDS             1068..2171
                     /locus_tag="PKV59_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQP45449.1"
                     /translation="MTASDLISLINGMRAANGLGALSVDSALMACAQNTALVMAQNNM
                     SWHIGDVSGRVSQFGYNNGNKAFATENFMVGPTTLSAIQAAWSDYDHMIPATNPSYCH
                     IGAGVAEVNGKVYYVVQAAYPADKKGCGYSAASSSSGGSSTGTGNSTTVIDMSQIIAS
                     IKIATPGPDGKTYHVVENGQSLWSIATAYRVTIEDIATWNNIVDIESISLGQKLLIPE
                     RGSAGQAPTSTVLPTVLPTADSEGKYRHVITEGETLSSIAKLWGTTIQELMRWNGIDE
                     STTLGVGWKLFIPVTPTVTVPPTLTPLPSETPSPTQTLTMTPLPSMTPTPTLIAANHL
                     PKASTKTYFIGIASIILLTTVFFFSGRYFYRRK"
     gene            complement(2248..3573)
                     /locus_tag="PKV59_01470"
     CDS             complement(2248..3573)
                     /locus_tag="PKV59_01470"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HQP45450.1"
                     /translation="MILEDRIQADVSTWRERVNRLTKEHGDFVISNVTVSQIFSGIRG
                     VPIQVSDISYVDPEKGIRYRGYTINEILSLLPKLPDTNYPLVGGVYHLLMGNELPTME
                     KALAIENEWKNRSEVPDYVFRIIDSFPENASTMAMYSAAILSMSSDSKFSKGYHDSLR
                     KEDYWKAMLDDSLDLTARSPQIAAYIYNKKYRNNEQIGPDKSLDYAANFAHMIGKGDD
                     PLYADLMRLFILLHADHENANVSAHTAHLVGSALSDIYLSCSAGLNGLAGPLHGLANQ
                     ECFNWLLALYNTYQGVPTKQQVTEYIQKTLDEGKVIPGYGHAVLRCIDPRFEAERQFA
                     EKYFPDDEYFRTAMVVFETAPDMLKATGKVKNPWPNVDALNGTLQYHFGVTQSDYYTV
                     LFGLSRMLGFTCHIVWARALGKPIERPKALTTRLLESMIAEEKKQEAAK"
     gene            complement(3618..4403)
                     /locus_tag="PKV59_01475"
     CDS             complement(3618..4403)
                     /locus_tag="PKV59_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HQP45451.1"
                     /translation="MAEKESYLHRVFALLDCFTAERPELGVREASRMLGISRSATGRL
                     MSELRNEGVLTQDLDTSLYSLGGRVIRWAGVYEAASDLSVKALPYIRQLLNKTNETVS
                     LYVVEGNERVCIERLESRQNVRIVEPIGQRLKLYAGSGGRAILAFMPPEEINRILKLA
                     EQEITEGDVQEQIREIREHLEEVRRTGVAISHGQWVDGASGIAAPIFDESGLPIGSVS
                     VSGPTNRFRDQKTLDAYRELLLSTMEKLSNEMGYRSDRIIANP"
     gene            complement(4597..7590)
                     /gene="ygfK"
                     /locus_tag="PKV59_01480"
     CDS             complement(4597..7590)
                     /gene="ygfK"
                     /locus_tag="PKV59_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HQP45452.1"
                     /translation="MSDRMTCIPFSQLLNWVFDEYQSQGTVFGVHQPYKAKEGKTLEL
                     FGRKLETPIGPAAGPHTQLAQNIISAYFAGSRFFEVKTVQIIDGEDLPVSKPCIDATD
                     EGYNIEWSTELTVPDAMGEYIKAWVILHLMAKEMGLGAQDGFQFNMSVGYDLAGIRSE
                     KINTFIDSMIEAKDTEVFKNAIRTAKANLSKFKHVTEADIDAIPSAICNSATISTLHG
                     CPPKEIESIATYLITEKHLNTFVKCNPTLLGYDFARKTLDEMGYDYVSFGRFHFEDDL
                     QYQDAVPMFERLIDLAASHHLQFGVKLTNTFPVDITRHELAGKEMYMSGKPLFALTSA
                     LAAKLSRDFDGKLRISYSGGADAFNIEKIVGAGIWPVTMATTILKPGGYQRFRQLAEK
                     LEKQGYHSFEGVNVAAISKIAEDAKKDKHYLKPAKMPPSRKNNRKVPLLDCFIAGCEE
                     GCPIHQDIPTYIRLTGEGKYKEALQVILDKNPLPFITGTLCAHPCMTNCVRNHYESPV
                     NIRGVKLEAAKQGYESVMRELKPTGHSEKNVAIVGGGPAGIAAAFYLLRAGAKVTIFE
                     KRPRLGGIVRYVIPRFRIDPTTVINKDLDFIRQLGVEIHLNTEVSSVEDLKTGLFDAA
                     IIAIGAHVPAQITIEGCTPLHALSFLEEFNNTKGKMNIGKNVVVIGGGNTAMDVARSA
                     KRTEGVENVYLVYRRTKRYMPADEEELQLALNDGVIFKELLAPEKWENGELICRVMEL
                     GEYDESGRRSVVETDQYVRVSADTVISATGENVSTDFYQANGIAVDAKGKPVVNPKTL
                     ESSKENIYIVGDGLYGPSTIVQAIANSMKAASAIVGFDVTSDVFQPTPKENIFPKRGV
                     LIKEEDATHESERCLSCSTVCENCVEVCPNRANVSIHVPGRSEAQIIHVDYMCNECGN
                     CETFCPYNSGPYKDKFTLFANEKDMENSKNAGFVVLDSKAKKFKVRLFGEESVITPES
                     RDFPEGIMDLMNSVCDNYPYLIFEK"
BASE COUNT         2155 a         1941 c         1624 g         2376 t
ORIGIN      
        1 ctccgactct ttccctcggt tccggaagcg gttccgcggc ctgtaccaat aaattcgaat
       61 ttttatcgga tgttacattc ccggatggaa caatgacatt gcctttgacc acttttacca
      121 aatcatggta cgtccaaaac accggtactt gtgaatggac caaatattac agtattgttt
      181 atttgtccgg agataatgtc ggaaccgcaa aggaatttcc gctgtttacc tcagataaac
      241 ttcttaaaaa cggggaatct gcgatcatat ccgtgataat taccgctccc gagaaacagg
      301 gagaatatag cacctattgg gcattgaagt cgcccgacgg acagatattt aaaggtggag
      361 attccgcgga agggtatccg ttatcgtcaa agttcaaggt gggatcgcaa tataactttt
      421 atgaaaatct ggctggtgcc atttgttcgg atgacagcgg tttgttcttc tgcggatcat
      481 ccgaccgttc atcgggcaga ggagtcgcgt attattccgc ttcaccgaca gtagagagca
      541 attatgcggg acaaccatcc atagtgatcg gtcccccctt aaaagaaaat ggcaaaacca
      601 gagtcacttt cggtccaatt cgagttcctc gcggtacatg gttacgggca gccgtatcct
      661 gtcctcccaa tgcaccgggt tgtgataact ttgttcgttt atttgtgcag gtggaaggaa
      721 gcactgaaca acgcgttaca gagactcaag aaaataacga tggctatgtg acggaatgga
      781 atatccaatt aagcgattat ggatatcatg atcaaagttt aacctacacc tttgaagtac
      841 aggcaaacgg cggcagcgat gcggatgatt taattattat tcaaatgcca cgcttaacag
      901 atgttgcgcc ggttaaatag gtgaagagta tttttcaaca attctgaatg cggaaaagtt
      961 ttttcttttt tttggttttt ctcctttttt tcagcctgaa ctgcaaagat cgggctgatt
     1021 ttttgcgacg gaattcgatt cagcccgttt atgccgccga aaattctgtg acggcatctg
     1081 atttaatctc tttgattaac ggaatgagag ctgcaaacgg tttgggagcc ctttccgtcg
     1141 attccgcatt gatggcatgc gcccaaaaca ccgccttggt catggcacag aataatatgt
     1201 cttggcatat cggagatgtc agcggcagag tctctcaatt tggttacaat aacgggaaca
     1261 aagcctttgc cactgaaaat ttcatggtcg gtccaacgac tttatcggca attcaggctg
     1321 cttggtcaga ttatgaccac atgattccgg ctaccaatcc gtcttactgt catatcggag
     1381 ccggcgttgc cgaagtcaac ggaaaggttt attatgttgt ccaagccgct tatccagccg
     1441 acaaaaaagg gtgcggttat tcggcagcat cttcttcatc cggcggctca tcgacaggaa
     1501 ccggaaactc gacaaccgtt attgacatgt ctcagattat cgccagtata aaaatcgcta
     1561 ctccgggtcc cgacgggaaa acctaccatg tagttgaaaa cggtcaaagc ctatggtcga
     1621 tcgctaccgc gtaccgtgtc acaatcgaag atattgccac ttggaacaac attgtcgaca
     1681 tcgaatccat cagcctcgga caaaaactgt taatacccga aagaggatca gccgggcaag
     1741 cgcctacttc caccgtttta ccgacggttt tgccgaccgc tgacagtgag ggaaaatatc
     1801 gacatgttat cacggagggg gaaacgcttt cttcaatcgc caaattatgg gggactacga
     1861 ttcaggaatt aatgcgctgg aacggtatcg atgaaagcac aacgcttgga gtcggatgga
     1921 agttgtttat tccggttact ccgactgtaa cagttccgcc gactttaaca cctcttccgt
     1981 cagaaacccc ttctccgacg caaactttga cgatgacacc tctgcccagt atgactccga
     2041 ctcccactct gatcgctgcg aatcatttgc cgaaagcaag cacgaaaaca tattttatcg
     2101 gaattgcttc tattatttta ttgacgacgg tcttcttctt ttccggaaga tatttttatc
     2161 gtcgcaaata gcgcatttct cgatcaatct atacacaaaa aaacagatat atttttcaat
     2221 atatctgttt tttcgttctg cttttgctta tttagcggct tcctgcttct tctcttcagc
     2281 aatcattgat tccaacaagc gtgtggtcaa tgctttcggc cgctcgatcg gctttcctaa
     2341 agctctcgcc cagacaatgt ggcaggtaaa tccgagcata cggctcaaac cgaacagcac
     2401 cgtatagtaa tcggactgag tcacaccaaa atgatactgc aatgtaccat tcaacgcatc
     2461 cacattcggc cacggatttt tcaccttgcc ggtggctttc agcatatcgg gcgctgtttc
     2521 aaatacgacc atcgcggtcc ggaaatactc atcatcgggg aaatattttt ccgcaaattg
     2581 tctttcggct tcaaaacgag gatcgataca acgcaatacg gcatgcccgt aacccggaat
     2641 aactttcccc tcatccaatg ttttctgaat atactcggtg acttgttgtt tggtaggaac
     2701 gccctgatag gtattgtaaa gggcaagcag ccaattaaag cattcctgat ttgcaagacc
     2761 gtgtaacggg cccgccaaac cgtttaaacc cgccgaacag gataaataaa tatcggataa
     2821 agccgaaccg accagatgag cggtatgagc actgacgttg gcgttttcgt gatcggcatg
     2881 cagcaaaatg aaaagcctca tcaaatcggc ataaagcggg tcatcgcctt ttccaatcat
     2941 atgcgcaaag tttgcggcat aatccaacga cttatcggga ccgatctgtt cgttgttacg
     3001 atattttttg ttgtaaatgt atgcagcgat ttgaggagat cttgcggtca aatccaaact
     3061 gtcatccaac atcgctttcc aataatcttc cttccgcagg gagtcatgat agccttttga
     3121 gaattttgaa tcggaggaca tggacagaat cgcagcggaa tacatcgcca tcgttgaagc
     3181 attttcagga aaagaatcaa tgattcggaa aacataatca ggcacttctg aacggttctt
     3241 ccattcattc tcaatcgcca gcgctttttc catcgtcggc aactcattcc ccatcaacaa
     3301 atgatagaca cccccgacta aaggataatt tgtgtccggc agcttcggga gaagagagag
     3361 aatttcgtta atcgtatatc cccgatatcg aattcccttt tcgggatcta cataagatat
     3421 atcgctcact tgaatcggta ctccccgaat tcccgagaaa atttgcgaaa cggttacatt
     3481 ggatatcaca aaatcgccat gttcttttgt cagccgatta accctttctc gccatgtact
     3541 gacatctgct tgaattctgt cctctaatat catttcattc ctcctgtatt tggatttgaa
     3601 ttcctctttt ttttgtttta tggattcgca atgatccgat cacttcgata tcccatttca
     3661 ttggataact tttccatcgt tgataagagc agttctcgat aagcatccaa tgttttttga
     3721 tccctaaatc ggtttgtcgg ccccgaaaca gataccgaac cgatcggtaa tccgctttca
     3781 tcaaatatcg gggctgcgat ccccgatgcg ccgtccaccc actgtccgtg actgattgcg
     3841 acaccggttc ttcttacttc ttcaagatgt tcccggattt cccgaatttg ttcctgaaca
     3901 tctccttccg taatttcttg ctcagccaat ttcaaaatgc ggttaatttc ttcgggaggc
     3961 ataaaagcca aaatcgcacg ccctcccgaa ccggcatata gttttaaccg ttgtccgatc
     4021 ggttccacaa ttcgaacgtt ttggcgactt tccaagcgtt cgatacagac ccgttcattg
     4081 ccttccacca catacaggct taccgtttca ttggttttat ttaataattg acggatatag
     4141 ggaagtgctt ttacactcaa atccgatgcc gcttcataga cccccgccca gcggattact
     4201 cttccgccca gactgtataa agaagtgtcc aaatcctgtg ttaatacacc ttcattccgt
     4261 aattcactca tcagcctgcc ggtggcactc cgtgaaattc ctaacatacg ggaagcttcc
     4321 cgcactccca attcgggacg ttctgccgta aaacaatcca ataaagcaaa tactcgatgt
     4381 aaataacttt ccttttcagc cattgcattt tgtcctgttt tacgggacag cgtcccgttt
     4441 gatgatgaat aaacaaatta taagaacatc ttcttgattt tgcaaggggt aattttttgc
     4501 attattacag atgagttttt tcggaatctt tcctttggaa aaataaaacg ggaaagaaaa
     4561 ttccttcccg ttttatctca ctcatcagtg atatttctat ttttcaaaaa tcaaataggg
     4621 ataattatca cagaccgaat tcatcaaatc cataatccct tccggaaaat cgcggctttc
     4681 gggggtaatg acagattctt cgccaaacaa acgaacttta aatttcttcg cttttgaatc
     4741 cagaacgaca aaaccggcat tcttactgtt ttccatatct ttttcattgg cgaaaagagt
     4801 aaatttatct ttgtacggtc cgctgttata cggacaaaat gtctcacagt ttccgcattc
     4861 gttacacata taatcaacat gaataatttg tgcttccgat cttccgggaa catgaatcga
     4921 tacattcgcg cgattcggac agacttctac acaattttca caaacggtag agcatgacag
     4981 acatcgctcg ctttcatggg tggcatcttc ttcttttatc aaaacgccgc gcttcgggaa
     5041 aatattttct ttcggtgtgg gttggaagac atcggaagtg acatcaaaac cgacaatcgc
     5101 agaagcggct ttcatactgt tggcaatcgc ctgaacaatc gtagaaggac cgtataaccc
     5161 gtccccgaca atatagatat tttctttgct ggattccaaa gtcttcggat ttacaaccgg
     5221 ttttcctttt gcatccactg cgattccgtt tgcttgatag aaatccgtag aaacattttc
     5281 acccgttgca ctgatgacgg tatcggcaga cacccttaca tattgatcgg tttctacaac
     5341 cgagcgccgc cccgattcat cgtattcgcc cagttccatt acccggcaaa tcaattctcc
     5401 gttttcccat ttttccggcg caagcaattc cttaaaaatt actccgtcgt tcaatgccaa
     5461 ttgcagttcc tcttcatcgg caggcatata tcgttttgtt ctgcgataaa ccaaatatac
     5521 attttccaca ccctcggttc gcttggccga tcgggctaca tccatggcag tattcccgcc
     5581 tccgatcacc acgacatttt tcccgatatt catcttgcct ttggtattgt tgaactcttc
     5641 caagaatgaa agcgcatgca gaggcgtaca tccttcgata gtgatttgag ccggaacatg
     5701 agctccgatg gcgataatcg cggcatcaaa aagtccagtt ttcaaatctt ccaccgaaga
     5761 tacttctgtg ttcaaatgaa tttcgactcc taattgccga atgaaatcca agtccttgtt
     5821 gatgactgtt gtgggatcga tccggaatcg cggaataaca tatcggacaa ttccgcccaa
     5881 ccgaggtcgc ttttcaaaaa tcgtaacctt cgcgcctgcg cgtaaaagat aaaaagctgc
     5941 cgcgataccg gcgggaccgc cgcctacaat tgcgacattt ttctccgaat gtccggtcgg
     6001 ttttaattcg cgcataacgc tttcatatcc ctgcttggca gcctcaagct taacgccgcg
     6061 aatattgacc ggtgattcat aatgattcct aacgcaattt gtcatacaag gatgtgcgca
     6121 taaagtcccc gtaataaagg gaagaggatt cttatccaaa atgacctgca aagcctcttt
     6181 gtattttcct tctcccgtca agcgaatgta ggtcggaata tcctgatgaa tcggacatcc
     6241 ttcctcacaa ccggcgataa aacaatccaa aagcggcact ttacggttat tctttcggga
     6301 aggcggcatt ttcgccggct tcagatagtg tttatctttc tttgcatcct cggcgatttt
     6361 agagatggca gcaacattta ccccttcaaa cgaatggtac ccttgctttt ccaatttttc
     6421 cgctaattgt cgaaatcgtt gatatccgcc cggtttcaga attgtcgttg ccatcgtcac
     6481 cggccatatt ccggcgccta caattttttc gatattgaaa gcatccgcac cgccggaata
     6541 agagatccgt aattttccgt caaaatctct ggaaagcttc gctgccagcg cgcttgtcaa
     6601 tgcaaaaagc ggtttaccgg acatatacat ttctttaccg gctaattcat gacgcgtaat
     6661 atcaaccgga aaagtattgg tcagtttgac accgaactga aggtggtgac tggcggctaa
     6721 atcaatcaat cgttcaaaca tgggaacggc atcttgatat tgaagatcat cttcaaaatg
     6781 gaatcgaccg aaggatacgt aatcatatcc catttcatct aaggtcttcc gggcaaagtc
     6841 ataccccaaa agtgtggggt tacattttac aaaagtattc aaatgctttt cggtgattaa
     6901 ataggttgcg atactttcga tttctttggg cggacacccg tggagagtgg atatcgtcgc
     6961 cgaattacaa atagcggacg gaatcgcatc gatatcagct tcggtcacat gcttaaattt
     7021 ggataaattt gctttagcgg ttcgaatggc attcttgaaa acttcggtat cttttgcttc
     7081 aatcattgaa tcgataaaag tattgatttt ttccgatcgg atgccggcga gatcataccc
     7141 gacgctcata ttaaattgga atccatcctg cgcgcccagc cccatctcct tcgccatcaa
     7201 atgcaatatt acccaagcct taatgtattc tcccatcgca tcggggaccg taagctctgt
     7261 cgaccattcg atgttgtaac cctcatcggt ggcatcgatg caaggctttg atacgggtaa
     7321 atcttcaccg tcgataattt gaacggtctt tacttcgaaa aagcggcttc cggcaaaata
     7381 tgcggaaatg atattctgcg ccaactgggt atggggaccc gctgccggtc cgatcggagt
     7441 ctcaagcttt cgaccgaaca attccagcgt cttcccttct ttggctttat acggttgatg
     7501 gaccccaaat acggtgccct gtgattgata ttcatcaaag acccaattca acaattgaga
     7561 aaagggtatg caggtcattc tatcgctcat cgtttttctc cttttcaatg actcgttatt
     7621 gataaaataa atatttgata attgctaacg ggaattcgta atcttaataa gataaaagtt
     7681 cattgatctt cccgctaaat tccatcattt tttccggttt tcatttcctt tataaattaa
     7741 atgatgatcc atgctgttac ggaatttatt ggatgaaaat cttttcatca atttcagttt
     7801 atcatttccg gtcggaattt gcaacttttt tggctattgt ctaacaacat attcttaatt
     7861 attttgtcat cttttttttc taacattttc aatcgaataa cttttcgcct tcatttttcc
     7921 tcaaaaattc gcgggcgatt catgaaaata tacttattaa tatcataatt gttaagacat
     7981 ttaccgagat tgtcttccgg gaaaaacatc aatacctatt cgaatcgaat ctttgaagga
     8041 acttatgcgg ataacggatt cagtttcaga tcccgttctt tttcttcgcg atactg
//
LOCUS       DAOMIV010000032         7833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_9359_length_7833_cov_4.973001, whole genome shotgun sequence.
ACCESSION   DAOMIV010000032 DAOMIV010000000
VERSION     DAOMIV010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9359_length_7833_cov_4.973001"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..4361
                     /locus_tag="PKV59_01485"
     CDS             195..4361
                     /locus_tag="PKV59_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45453.1"
                     /translation="MFTKLKTALGLDPIQKKLEEYTAIVSEINRLEPDYEKLSDEALR
                     AKTDEFKDRLANGETLDDILTEAFATVREASKRVLGQRPYDVQLIGGIALHKRNIAEM
                     KTGEGKTLVATLPVYLNALAGKGVHLVTVNDYLARRDARWMGRIYQALGLTVGVLQMA
                     ARTDNGRKAFLVDYSIESPHEDQDHLRLVPRKEAYDADVTYGTNSEFGFDYLRDNMVM
                     KLEDRVQRGHSYAVIDEVDNVLIDEARTPLIISGPASDDVDAYYQMAKVVKQLSPDEY
                     EVSERDHNVYLNDNGMDHVEELLGMVLRDPSRPEEVTIEQERMMGYLNQALAAEFLYH
                     RNKEYLVQSGKVIIIDSSTGRLMPGRRWSNGLHQAIEAKEGVKVEPENMTYATITLQN
                     YYRMYDKIAGMTGTALTEKEEFYKIYLLDVIPIPTNLEYTARQKNSNLIEREAKDEEG
                     YKYTYYADPAAPDIPLYYKRKDYPDVIYQSEEAKFRAITIEILKNHVIGRPQLVGTAS
                     VEHSEYLASRLKQEPLRRLVQILMLRRAWMKQNNIEVLESPLKEFIPFNKPIQEINAG
                     DLRPMAKQLGVSLNVDDPDNRSLLMEEFGLSESNVDRFIEVVESGMNPQVLNARKHDE
                     EGMIIAKAGALGAITIATNMAGRGVDIKLGGELDEERIRDTNRVLTKMGIDPYNMTLD
                     ERYQAILKVPPEEYGVYEESVKAYIDYIDQMEKVRDLGGLHVIGSERHESRRIDNQLR
                     GRAARQGDPGSSRFFLSLQDEIVRLFGGEQLEGVLKRVNLLDVNVPLENNLFSRMIEQ
                     SQERVEGANFDARKHTLEYDDVLNSQRKRIYEQRDQAFVKEDLSEDVHAMLETDLDNR
                     LDKAMDEEKWKLALYLDSIQPTIEVEENYLPSFSQSLLIQSLKEKVGSAPEKENLLNA
                     LDELSREAFRRENEVGLEQMETLIRNSQSGYESQLEERTANFELFVDSLKERLKEQQE
                     AKEEGRVVEPIRPQDLLTEAGNIARVGFKLSPDKLRKLAEGDADIIGELRSQIEIALF
                     AGYIQRLNQLIENRMIGDYEPPTTKFEIGDWEGFENAVMDAVQKAFRTRAERLFGNQG
                     QVKSDLESALRTYQPAELTDKQWVQLFRTISQGQRIGFDAKTHRKTSRIFTRINLVYT
                     AGDLLKDLDREELKELIWNHYMNAEDGLRQIFGKLDWGRLRSYNIPLSQLKPETQEKI
                     SRAIGSEIFEEVKEIPPAEMDGEIAAEIQSYLGKRIQNEVNRQVILRSITGQWVDYLT
                     QIEGLRVSISMESYAQRNPLVVYKTKAAELFSQLLNDIRRSVVEQMFVTLPQLSMLTA
                     AERISLADLRQQEPSPQESNAEPVESDSDRSDDGQISPDETAPKEEPREREVVQASSP
                     SKKKKKKMKK"
     gene            4358..5356
                     /locus_tag="PKV59_01490"
     CDS             4358..5356
                     /locus_tag="PKV59_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQP45454.1"
                     /translation="MSWDIFGHDQVVNILRNHILQDEVRHAYLFVGIPGTGRRTIALQ
                     FAQALNCSEPPAPGEFCGKCRNCRQIAAQQHPDLIVTETETVGTILKIDEIRDLQHTL
                     SLAPYESRWRIGLLLRFDEANQNAQNALLKILEEPPENVKLLLTADSENSLLPTITSR
                     CEVMHLFPANPAQLTSYLTDRYQVESKAAESAAHLSAGRVGFAISLLKEPEKAEQMNR
                     TAAECLEMFSQNTRERFRYASNFKDYKKRTELREVLQVWQSVMRDLLLLSTGGKLTAQ
                     SLTFIDLCSESASVAAKYSTEQFRELIRHLNRSLEYLDANVSPQLLLEELLLNFPS"
     gene            complement(5391..6137)
                     /locus_tag="PKV59_01495"
     CDS             complement(5391..6137)
                     /locus_tag="PKV59_01495"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45455.1"
                     /translation="MIASTENLGMTFPSENGGLQALSDINLTLYDREFVCIIGPSGSG
                     KSTLLRILADVLVPTQGKVTFADGQNPNRAIVFQSSNLLPWMNVIQNIALPLKIRGAG
                     EKEALSQAGNWIRRVGLNGFEYEWPQNLSGGMKQRVAIARALVQQPELLLLDEPFGSL
                     DALTRDQMGLELLKTWETFHPTVLMVTHSITEAVLLADRVAVFSKRPGQIIHELEIPF
                     QRPRSPELRDEIKFLQVVKKLRGMIVTESI"
     gene            complement(6161..7147)
                     /locus_tag="PKV59_01500"
     CDS             complement(6161..7147)
                     /locus_tag="PKV59_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45456.1"
                     /translation="MIYKTIKKLLSVLLTVLFLMTGSNRVFAEEFSLPVGFVPNVQFA
                     PLYVALENGYFAEENLKITLDHSLETDTVALVGANKIPFGICSGEQVLLARNQGLPLT
                     YIAEWYEQYPVGIVSLKEAGITSMQDLRGKKVGVPVLSGASYIGLEALLQQSNMTDAD
                     IQLETVGYTQVELLISGKIDAAVIYIANEPEQLKALGYEINLIRVADTVPMVSNGLIT
                     NEKTIRENPELVRRMTRAFLKGIQWTRQNPDQAYEICKKYVDNLANAENQELQKQILI
                     QSIALYQSPSSETPLGTTDAAAWEKMVEVMKQMKLLKPDFDMSGSFTNEFLP"
     gene            complement(7168..>7833)
                     /locus_tag="PKV59_01505"
     CDS             complement(7168..>7833)
                     /locus_tag="PKV59_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45457.1"
                     /translation="PFILPAPSRIFSRLIESIASGVLLYHTGITLVEVLTGSITGTIL
                     ALLTGYLLAHHPIAENVLFPYIVASQSIPMAAIAPLLIIWFGSGIMPKILICALVVFF
                     PIQINVIIGLRDIQRNFLDLMKSMHANRKAQLKYLEIPAALPVVFGGLRISATLSVIG
                     AVVGELTGADAGLGYLINTARGQYDTAMVFVAVFMLMIISILLYVLILAIEKRTLRWQ
                     TVQ"
BASE COUNT         2191 a         1680 c         1965 g         1997 t
ORIGIN      
        1 ccgatgaaat catttttggg acttattgac cgtttcgatt gggtatggca atcggcagaa
       61 aaaattgact ttgtccgaag gatttttgat gctctttgag acaaagcctt tgttgtttga
      121 tttgcttcaa gtggtaagat taacgcagaa ttttgaggaa agcgggtgat ttgttcaccc
      181 gatgtgggag atttatgttt acgaagttaa aaacagcgct cggattggat ccgatccaga
      241 aaaaattgga agaatatacc gcgattgtct cggaaattaa tcgtctcgaa ccggattatg
      301 aaaaactgag cgatgaggca ttgcgtgcca aaaccgatga atttaaagat cgattggcaa
      361 acggagaaac actggatgat attctgacgg aagcatttgc cactgtcagg gaagccagca
      421 aacgagtttt ggggcagcgc ccttatgatg tgcagttgat cggcggtatt gctttgcata
      481 aacgaaatat cgccgagatg aagaccggtg aaggaaaaac gttggtagcc actcttccgg
      541 tttatctgaa cgcattggcg gggaaaggtg ttcatttggt tacggtgaat gattaccttg
      601 ccagacgcga tgcccgttgg atggggcgta tttatcaggc gctcgggttg acggtgggcg
      661 tgctgcagat ggcggcgcgg acggataacg gcaggaaagc cttcctggtc gactacagta
      721 tcgagtcgcc tcatgaggat caggatcatt tgcgattggt tccgcgaaaa gaagcttatg
      781 atgccgatgt tacctatggc acaaacagcg agttcggttt cgattacctg cgggataaca
      841 tggtaatgaa gctggaagat cgggtacaac gcggtcactc ttatgcggtg atcgatgaag
      901 tggataatgt gttgattgac gaagcgcgaa cgccgttaat tatctccggt ccggcttcgg
      961 atgatgtgga tgcttattac caaatggcaa aagtggtcaa gcaattgtca ccggatgagt
     1021 atgaggtcag cgaacgggat cataacgttt atctgaacga taacgggatg gatcatgtcg
     1081 aagagttgtt ggggatggta ttaagggatc cgagccggcc ggaagaagtg acaattgaac
     1141 aggaacggat gatggggtat ctgaatcagg ctttggcggc cgaatttctc tatcaccgga
     1201 ataaagaata ccttgtccag tcggggaaag tgatcatcat cgacagctcc accggccgtt
     1261 taatgccggg gcgacgttgg tccaacggtt tgcatcaggc gattgaagcg aaagaagggg
     1321 taaaggtcga gccggagaat atgacctatg caaccatcac gctgcaaaat tattaccgga
     1381 tgtatgacaa gatcgccggc atgactggta cggctctgac cgaaaaagaa gaattctata
     1441 aaatttatct gttggatgtc attccgatcc cgaccaacct tgaatatacc gctcgccaga
     1501 aaaacagtaa cctgatcgag cgcgaggcga aagacgaaga aggttacaaa tatacctatt
     1561 atgccgaccc tgccgcaccg gatattccgc tttactataa gcggaaggat tatccggatg
     1621 tcatttatca gagtgaggag gcgaagttcc gtgcgatcac aatcgaaatt ctgaaaaatc
     1681 atgtaatcgg ccggccgcaa ttggtcggta cggcttccgt cgaacattcc gaatatttgg
     1741 caagtcgact gaaacaagaa cctttgcgtc ggctggtaca gattttaatg ctgcgccggg
     1801 catggatgaa acaaaataat atcgaggttt tggaatcgcc gcttaaggaa tttattccgt
     1861 ttaataaacc catccaagag atcaatgccg gcgatttacg cccgatggca aaacagttag
     1921 gtgtatcgct gaatgtcgat gatccggata accgatcgct tctgatggaa gaattcgggt
     1981 tgagcgaatc gaatgtcgat cggtttattg aggttgtcga aagtggtatg aatccgcagg
     2041 tattgaacgc ccgcaaacac gatgaagagg gaatgatcat tgccaaagcc ggtgccttgg
     2101 gtgccatcac gattgccacc aatatggccg gacgcggtgt cgatattaaa ctgggcggag
     2161 aattggatga agaacgcatt cgggatacca accgtgtttt gacgaaaatg ggcatcgatc
     2221 cttataacat gaccttggat gaacgttatc aagcgattct gaaagttccg cccgaagaat
     2281 acggtgtata cgaggaatcg gttaaagcct atatcgatta catcgaccag atggagaaag
     2341 tccgtgattt gggcgggtta cacgtgatcg gttccgaaag acacgaatct cgtcgaatcg
     2401 ataaccaatt aagaggacgg gcagcccgcc agggcgaccc cggttcttcc cgcttcttcc
     2461 tgtcgctgca agatgagatt gtccgtctct ttggcggaga gcagcttgaa ggagtattaa
     2521 aacgggtaaa tttactggat gtgaatgttc cgcttgagaa taaccttttt tccaggatga
     2581 tcgaacaatc ccaggaacgg gttgaaggcg ctaactttga tgcccggaaa catacattgg
     2641 aatatgatga tgtacttaac tcgcagcgca agcggattta tgaacagcgg gatcaggctt
     2701 tcgtcaaaga agatttaagc gaagatgtgc atgccatgct tgaaacagat ttggataatc
     2761 gtctggataa agcaatggat gaagaaaagt ggaaactcgc gctttatttg gacagcattc
     2821 aaccgacgat tgaggttgaa gagaattatt tgccctcttt ctcacaatct ctgctgattc
     2881 aatctttaaa agaaaaagtc gggtccgccc cggaaaaaga gaatctttta aatgctctgg
     2941 atgaattaag ccgggaggct tttcggcgag aaaatgaagt gggtttggag cagatggaaa
     3001 cgctcattcg taattctcag agcggttatg aaagtcaact ggaggagcga acagctaatt
     3061 ttgagctgtt cgttgattcg ctgaaagaaa ggctcaaaga acaacaggaa gccaaagaag
     3121 aagggcgagt ggtcgaaccg attcgaccgc aggatctgtt gacggaagcc ggaaatattg
     3181 caagagtcgg attcaagctt tcaccggaca aactgcgcaa attagccgaa ggagatgccg
     3241 atatcatcgg agaattgcgc agtcaaattg aaatcgcttt atttgcgggc tatattcaac
     3301 gtctgaatca attgattgaa aatcggatga tcggtgatta tgaacctccg acaaccaaat
     3361 tcgagatcgg tgattgggag ggtttcgaaa atgcagttat ggatgcggtt cagaaggctt
     3421 ttcgtacccg ggcggaacgt ctgttcggaa atcagggaca ggtgaagtcg gatttggaaa
     3481 gcgctttacg cacctaccaa ccggctgaac tgacggacaa acaatgggtt caactctttc
     3541 ggacgatcag tcagggacaa aggatcggtt tcgacgccaa aactcatcga aaaaccagtc
     3601 ggatatttac gcgcattaat ttggtttata cggccggtga tttattaaag gatttggatc
     3661 gggaagaact gaaggaattg atttggaatc attacatgaa tgccgaagac ggattgaggc
     3721 agattttcgg aaaattggat tggggaagat tgcgatccta taacattcct ttaagccagc
     3781 taaaaccgga aacccaggaa aaaatcagca gggcaatcgg atcggaaatt ttcgaagaag
     3841 tgaaagaaat tccgccggct gagatggatg gcgaaatcgc ggctgaaatc caatcctatt
     3901 tgggaaaacg aattcaaaat gaagtaaacc gtcaggtaat tttacgaagt attaccggcc
     3961 aatgggtcga ttatttgact caaatcgaag gattacgtgt ttcgatcagc atggaaagtt
     4021 atgcccaaag gaacccgttg gtggtttata agaccaaagc ggcggagctg ttctcgcaac
     4081 tgttgaatga tattcgtcgc agcgttgtgg aacaaatgtt tgtcacctta ccgcaattat
     4141 ctatgctgac ggcagcggag cggatcagct tagcggattt acgacaacaa gagccgtctc
     4201 ctcaggaatc aaatgccgaa ccggttgaaa gcgattcgga ccgatcggat gacgggcaaa
     4261 tatctccgga cgaaaccgcc ccaaaagagg aaccgcggga aagggaagtt gttcaggctt
     4321 cttcgccgtc caagaagaag aaaaagaaaa tgaaaaaatg agctgggata ttttcgggca
     4381 tgatcaagtc gtaaatatcc tgcgcaatca tattttgcag gatgaagtcc ggcatgctta
     4441 tctgtttgtc ggaattcccg ggacgggtcg ccggacaatt gcccttcagt ttgcacaggc
     4501 tttaaattgt tcggaaccgc ccgccccggg tgagttttgc ggaaaatgtc gcaactgtcg
     4561 acagattgcc gcacagcagc atcccgattt gattgtgacc gagacggaaa ccgttggaac
     4621 catactgaaa attgacgaaa tacgggatct gcaacatact ttatcgttgg cgccttatga
     4681 gtcccgctgg cggatcggtc ttttgctgcg ctttgacgaa gccaatcaaa atgctcaaaa
     4741 tgctctgttg aaaattttgg aagagccccc tgaaaatgtg aaattattgt tgacagctga
     4801 ctccgaaaat tctctcttgc ctaccattac atcccgttgc gaagtgatgc atttgtttcc
     4861 ggccaatccg gcgcagttga caagctattt aacggatcga tatcaggtcg aatcgaaagc
     4921 ggcggaaagc gccgctcatc tgtctgccgg ccgggtcggg tttgcgattt cgctcttaaa
     4981 agaacctgaa aaagcggagc aaatgaatag aacggccgcg gaatgccttg aaatgttctc
     5041 acaaaatacc cgcgaacgat ttcgatatgc ttccaatttt aaggattata aaaaaagaac
     5101 ggaattgcgg gaggtattgc aggtttggca atctgtcatg cgggatttac tgttgctttc
     5161 taccggcggt aaactgaccg ctcaatcgct cacttttatt gatctctgtt cggaatcggc
     5221 ttcggtagca gccaaatatt caaccgaaca atttcgcgaa ttgatccggc atttgaatcg
     5281 gtcgttggaa tatttggatg caaatgtcag tccgcaactg ttgttggaag agttgctgct
     5341 aaattttccg tcatgatcca aaaaaaattt tgcggtcgca aagctttgtt tcagatactt
     5401 tcggtaacga tcattcctct taatttttta acaacctgca aaaattttat ctcatccctt
     5461 aattcgggcg accgcgggcg ttgaaagggg atttccaact catgaataat ctgtccgggg
     5521 cgtttgctga aaacggctac ccgatccgct aacaaaaccg cttccgtaat cgaatgggtt
     5581 accattagaa cggtcggatg aaacgtttcc caggtcttca aaagttccag ccccatctga
     5641 tcacgtgtca gggcatccaa ggaaccaaac ggctcatcca aaagaagcag ttccggttgt
     5701 tggactaaag cgcgggcgat cgcaactcgt tgtttcatcc ctcccgataa attttgcggc
     5761 cactcatatt cgaatccgtt cagtccgacc cgacgaatcc agtttccggc ttgagagagc
     5821 gcttcttttt cgccggctcc ccgaatttta agcgggagcg ctatattttg gatgacattc
     5881 atccatggga gtaaattgga actctgaaaa acgatggcac gattgggatt ttgcccgtcg
     5941 gcaaaagtta ctttcccctg tgtgggaacc aacacatccg ccaatatccg cagtaaagta
     6001 cttttccctg agccggaagg accgatgata cagacaaatt cccgatcgta aagcgtcaga
     6061 ttaatatcac ttaaagcttg cagtccgccg ttttccgatg ggaaagtcat tccgagattt
     6121 tcggtggaag cgatcacttt tcgcaaagag tacctgccgg ctacggtaaa aactcgttgg
     6181 taaacgaacc gctcatatca aaatcgggct tcagcagctt catctgtttc ataacttcaa
     6241 ccattttttc ccatgcggcc gcatccgttg taccgagcgg tgtttcggaa gacggacttt
     6301 gatataacgc gatggattga attaatattt gtttctgtaa ttcctggttt tcggcatttg
     6361 ccaaattatc gacatatttt ttacagattt cgtaagcctg atcgggattt tgtcgagtcc
     6421 attgaattcc tttcagaaaa gcacgcgtca ttctccggac aagttccggg ttctcacgaa
     6481 tggttttttc attggtgatc aaaccgttac tgaccatcgg gacagtatcg gcaacccgaa
     6541 taaggttaat ctcatatccg agtgctttca attgttcggg ttcgttcgcg atataaatta
     6601 ccgcagcatc gatttttccg ctgatcagca attccacttg ggtataacct accgtttcca
     6661 actgaatatc agcatccgtc atattgctct gctgtaataa tgcttccaat ccgatataac
     6721 tggctccgga gagaacgggt acacctactt ttttcccgcg caaatcctgc attgaggtaa
     6781 ttcccgcttc tttcagcgag acaattccca ccggatattg ttcataccat tccgcaatgt
     6841 aagtcagcgg aagtccctga tttctggcga gcagcacttg ttctccggag caaatgccga
     6901 aaggtatttt gtttgcgccc accaatgcga cggtatccgt ttcaagactg tgatccagtg
     6961 tgattttcag attttcctcg gcgaaatacc cgttttcaag tgcgacatat aacggagcaa
     7021 actgaacgtt ggggacaaat cctaccggta atgagaattc ttccgcaaag actcggttag
     7081 aaccggtcat gagaaacaag acggttaata gaaccgataa cagcttttta atggttttat
     7141 aaatcatttt tcctccgatt ttccgattta ttgaacagtc tgccaacgta aggttcgttt
     7201 ttcgattgcc agaatcaata catagagcaa aattgaaatg atcatcaaca taaataccgc
     7261 gacaaaaacc attgcggtat cgtactgtcc tcttgcggta ttgatcaaat atcccagccc
     7321 ggcatccgca ccggtcagtt ccccgacaac ggctccgatg acggaaagcg tcgcactgat
     7381 tcgtaatccg ccgaaaacca ccggcaacgc agccggaatt tccaggtatt tcagttgtgc
     7441 tttccgattt gcatgcattg atttcatcag atccagaaaa ttcctttgaa tgtcacgtaa
     7501 gccgataatg acgttgattt gaatggggaa gaagaccacc agggcacaaa tcagaatttt
     7561 cggcattatt cctgatccga accaaatgat caatagggga gctatcgccg ccatcggaat
     7621 ggattgagac gcgactatgt aggggaacag aacattttcc gcaatcggat gatgagctaa
     7681 cagatatccg gtcagcaatg ccagaatcgt accggtgatc gaaccggtca aaacttcgac
     7741 caatgtaatt ccggtatgat ataaaaggac gccgcttgcg atcgattcga tcaaccggct
     7801 gaaaattcga gaaggtgccg gcaatataaa cgg
//
LOCUS       DAOMIV010000033         7794 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_9416_length_7794_cov_4.807210, whole genome shotgun sequence.
ACCESSION   DAOMIV010000033 DAOMIV010000000
VERSION     DAOMIV010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9416_length_7794_cov_4.807210"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKV59_01510"
     CDS             <1..319
                     /locus_tag="PKV59_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQP45458.1"
                     /translation="SQILSGELKEGDKLPSLRELARDLRISVLTTTRVYTELEKEGFI
                     TNIQGRGCYVLGEGSELLKEHMLREMERNLSDALKAAKIAGLDISEVHQALDLMNEEK
                     QNE"
     gene            312..1169
                     /locus_tag="PKV59_01515"
     CDS             312..1169
                     /locus_tag="PKV59_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45459.1"
                     /translation="MSDAINVKNISKIYPGFTLKDITFDLPKGYIMGFIGPNGAGKTT
                     TIKLILNMIQRDGGTVDVFGLENIAHDAEIKEQTGVVFDNHYFVDEWTLNDINRVMAL
                     FFSNWQSSIYQKKLEQYQLNPVKKVKELSKGMRLKLMLAVAFSHDAKLLILDEPTSGL
                     DPVARDELLDTLAEFIQDGEHSVLFSTHITSDLEKVADYITFINDGRLIYTGIKDEFI
                     DSFRLVKGGKEDLTPDVKAHLIGYREYGSGFEGIMYTEYANYFPNLLSEKSSIDNIIV
                     FTYKGTNGL"
     gene            1166..1339
                     /locus_tag="PKV59_01520"
     CDS             1166..1339
                     /locus_tag="PKV59_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45460.1"
                     /translation="MKDILNALRFNFILVKPYLRYYLMGFLVVFFFKIMGSEIDAVSF
                     MLFMLLSTHLYPF"
     gene            1340..1789
                     /locus_tag="PKV59_01525"
     CDS             1340..1789
                     /locus_tag="PKV59_01525"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HQP45461.1"
                     /translation="MEEKNDLYRLYGIAPIKRSVLIFGRYLFIVVISLIDWLLAMMME
                     YAFSKSTIIISDYLISSLFIFSIFLILTAFEIPGLYKLGVIKGRWIVYVPLVLMFIIV
                     FLADAFQILESAMHWITANFWLVPTVTIIVLSLSISLSVRIFNQKEF"
     gene            1897..2811
                     /locus_tag="PKV59_01530"
     CDS             1897..2811
                     /locus_tag="PKV59_01530"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQP45462.1"
                     /translation="MLDFLRKEGIRSDLISQIELFREKYPVSPEKKYRIPQPKYYYYG
                     NDIWEEAITALLCGENLLLVGPKATGKNVFAENLAAAFGRPSWDVSFHINTDSSSLIG
                     IDTLEEGRVLLRHGPIYECARWGGFGILDEINMAKNEAMAVLHASLDFRRIIDVPGYD
                     KFILADSTRFIATMNYGYAGTRELNEALTSRFMVIEMPVISQKNLKRLLLREFPTINE
                     KYIEQFSGLFQDLQKKSENSEISTKPVDLRGLLAAIHLMDRGLEADKALQMGITNKTF
                     DDFERKLVEDVIRLRIPAGLERDAIFKD"
     gene            2811..4544
                     /locus_tag="PKV59_01535"
     CDS             2811..4544
                     /locus_tag="PKV59_01535"
                     /inference="COORDINATES: protein motif:HMM:NF023203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45463.1"
                     /translation="MIKTELQENRRASNIIWNAAFDYSFRPEVKYFDQNGKAELYGNY
                     IIGGAHYYYDFPLLEEFFEKLKQDADADFLRGLTWLGLENSTFLKGRKDRPVLEELRR
                     NFANTILENYSVTADSDILDDLKVAHFQRVLGKQPVMSETVANVLDQLEFDESLTTEQ
                     IIEKLNAVILNYFQFQKAEYEKNRLAFLQKNKTVYQWNETNRKRFLRIPFLRNPKNEP
                     LEFPLATNSDRVPVRKNILTSYWRKYRNANEINQEKYIREYYGKPVLSDSEVKILEKK
                     LCTGAHKNCRLHFTRGEIDVSGNLSEQQSVILMQREKNRAYFRKNLAVNNTGIIKLTE
                     KIRNSMLLMPSSFSGRANAGRLTPERAWRNLYIHDKNVFQKKIQNEIGDLSVDILLDA
                     SASQLGRQEAIATQGYIIAESLTRCQIPVRVYSFCTKRKFTIMTLFRDYDEIYDNDKI
                     FNYFSSGCNRDGLAIRTAIHMMKNSPYQHKLLIILSDAKPLDTNIVPTTGKTQVEADY
                     TDLVGLNDTAAEVRKSRDFGISIMCVFTGMDEDLPSAKRIYGHNFVRIKGLERFADSV
                     GILIQNELKWL"
     gene            4720..5586
                     /locus_tag="PKV59_01540"
     CDS             4720..5586
                     /locus_tag="PKV59_01540"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQP45464.1"
                     /translation="MEKRRLGRTEMMISPVVYGGIIHMGETQETANRYVSYAVDRGVN
                     YFDVAPSYDDAEQLLGVALKPYRKNVFLACKTMERTASGSLRELENSLKTLQTDHFDV
                     YQLHSITTAEDVDTIFGPDGAMETFLSAKKKGIILNIGFSAHSEEQALRAMDLFDFDT
                     VLFPMNWALGLVRGWGDRVAQKVRESDKGLLCIKTLVDRKWREGENRDEFPKSWCHPT
                     TDNEKTGMAGMKYAFSKGATALVPPGDFKSFNFMLDHIDQVLQNPLNETDWIYLQEEA
                     QKIKDELIFDNQ"
     gene            5602..6351
                     /locus_tag="PKV59_01545"
     CDS             5602..6351
                     /locus_tag="PKV59_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQP45465.1"
                     /translation="MNQTITHQLNHRSIREFTDEPVKPEDLKLVLEAANHTATSTGMQ
                     SAAMIRVTDMAKKQALSEICQQPYVARFPELIIFIVDVYRNARIAEDNGYQFESERDM
                     DRFFQGWTDACLMAQNATNAIESLGMGAVFLGSILNDSAATIQVLKLPKLTFPVLGVG
                     FGWPNQNPQLKPRLPLDLRVFENEYKILADYKEAVREYDEEMQTYYDLREANRRVDSF
                     TLQVIKKLQNPSEKRAKILQVVRDQGFDLKV"
     gene            complement(6568..7464)
                     /locus_tag="PKV59_01550"
     CDS             complement(6568..7464)
                     /locus_tag="PKV59_01550"
                     /inference="COORDINATES: protein motif:HMM:NF033581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HQP45466.1"
                     /translation="MEKMGVIDRAILSEGQTWATAERDRNNAEEYLLQVWFSLSDEGI
                     EDSIYDSFAMRQFMHINFFTEQVPDATTLLKFRHLLEKEKIGEKIFEDVKDQLDNAGL
                     IMHGGTVVDATLISAPSSTKNEDGKRDPEMHQTKKGNQWYFGMKVHCGVDAGTGYVHT
                     ITGTSANVHDSAETAKLIREDDTVVYGDSGYLGVAQHEEIKNDPHLSAIDFRINRRPS
                     SIKLQDGTLGINWDKYIESRKSSVRAKVEHPFLIVKKQFGYFKVAYRGIAKNMNRFHV
                     LFASANLVMCIRGGRTEEFCNA"
BASE COUNT         2287 a         1450 c         1736 g         2321 t
ORIGIN      
        1 aagtcaaata ctttccggtg aattaaaaga aggagataag ttgccttcgt tgcgggaatt
       61 ggcacgggat ttacggatca gcgtgttgac tacaacgcgg gtctataccg aactggaaaa
      121 agaaggattt attaccaata ttcaggggcg cggctgctat gtcctcggcg aaggatcgga
      181 attactgaaa gaacatatgt tgcgtgaaat ggaacgaaat ttatctgacg ctttgaaagc
      241 ggcaaagata gccggtctgg acatatcgga ggttcatcag gcgttggatt taatgaatga
      301 ggaaaaacaa aatgagtgat gcaatcaatg taaaaaatat ctcgaaaatt tatcccggtt
      361 tcacattaaa agatattact tttgatttgc cgaaaggcta cattatggga tttatcggtc
      421 ccaacggagc gggaaaaaca actacgatca aattgatttt gaatatgatt caacgggacg
      481 gcggaacagt tgatgttttc ggcttggaga atatcgctca tgatgccgaa attaaagaac
      541 aaaccggtgt ggtatttgat aatcattatt tcgtcgatga gtggacgctt aacgatatta
      601 atcgggtgat ggctttgttt ttcagcaatt ggcaaagttc aatctatcag aaaaagttgg
      661 aacaatatca attaaatccg gttaaaaaag taaaagaatt gtcaaaagga atgcgattaa
      721 agttgatgct cgcggtcgct ttttcgcatg atgccaaact gcttattttg gacgaaccga
      781 ccagcggttt ggatcctgtc gcccgcgatg aactgttgga tactttagcc gagtttattc
      841 aagatggaga acatagcgtt cttttttcga cacatatcac cagcgatttg gaaaaggttg
      901 cggattatat cacttttatc aacgacggtc ggctgattta caccggcata aaagatgagt
      961 ttatcgattc tttccgcttg gtaaaaggag gaaaagaaga tttaactccc gatgtgaaag
     1021 cgcatttgat cggttatcgg gaatatggca gtggattcga aggaattatg tataccgaat
     1081 atgccaatta ttttccgaat ttattgtcgg aaaaatcttc gattgataac atcattgttt
     1141 ttacctataa agggacaaac gggttatgaa agatatttta aatgccttac gatttaattt
     1201 cattttagtg aaaccctatt tgcggtatta tctgatgggg tttttggtgg ttttcttttt
     1261 caaaataatg gggagtgaga ttgatgcggt ttcttttatg ttgtttatgt tgttgtcgac
     1321 tcatctttat cccttttaat tggaagagaa gaacgattta taccggctgt atggcattgc
     1381 tcccatcaag agatccgttc tgatttttgg tcgttatctc tttattgtag tgatctcgct
     1441 gattgactgg cttttggcga tgatgatgga gtatgccttt tcaaaatcca cgataattat
     1501 ttcagactat ctgataagca gtctgttcat tttttcgatt tttctgatcc tgaccgcttt
     1561 tgagatcccg ggcttatata aattgggggt gattaaggga agatggattg tttatgtccc
     1621 tctggtttta atgtttatta tcgtattttt ggcggatgca tttcaaattt tggaatcggc
     1681 gatgcattgg attactgcaa atttctggct ggtcccgacg gtgacgatta ttgttttatc
     1741 gctttccata tctctttccg ttcgaatttt taaccaaaaa gaattttgat cgattatttt
     1801 tcctcaacct tctcctttcc tatagcagaa ggttgagttt gtatttataa tgaaaaggat
     1861 gattaataca tatcccgatt atgggaggta tactgtatgc tggatttttt gagaaaggaa
     1921 ggaatccgtt ccgatctgat ctctcaaatt gagctttttc gagaaaaata tccggtaagt
     1981 cccgaaaaaa aatatcgaat tccccaacca aaatattatt attatggaaa tgatatctgg
     2041 gaggaggcga ttacagccct tctctgcggt gaaaatcttt tattggtcgg gcccaaagcc
     2101 accggcaaaa acgtttttgc ggagaatctt gcggcagctt tcggcagacc cagctgggat
     2161 gtttcgttcc atattaatac agattcctct tctctgatcg gaattgatac gttggaagag
     2221 ggcagagtgc tgctgcgaca cgggccgatt tatgaatgtg cgcgttgggg cggattcgga
     2281 attttggatg aaattaatat ggcgaagaac gaagccatgg cagtgctcca tgcaagtttg
     2341 gattttcgcc ggatcatcga tgtgccgggg tatgataaat ttattttggc ggactcgacc
     2401 cgctttatcg cgaccatgaa ctacggttat gccgggacac gggaactgaa cgaagcgctg
     2461 acatctcgat tcatggtgat tgaaatgccg gttatctctc aaaagaatct gaaacggtta
     2521 ttgctgcgtg aatttccgac cattaacgag aaatatattg aacagttctc cggattattt
     2581 caggatctgc aaaaaaagag tgagaactcc gagatttcga cgaagcctgt ggatctgcgc
     2641 ggtttattgg ctgcaattca tttaatggat aggggattgg aagccgataa agctttacaa
     2701 atgggaatca ccaataaaac ctttgatgat ttcgaacgaa aacttgttga ggatgtgatc
     2761 cggctcagaa tccctgccgg tttagaaagg gatgcgattt ttaaggattg atgataaaaa
     2821 cggaattaca ggaaaatcga agagcatcca atattatttg gaatgcggcg tttgattact
     2881 cttttcgccc cgaagtcaaa tatttcgatc aaaacgggaa agccgaatta tatgggaatt
     2941 atattatcgg aggggcacat tattattatg attttccgct gcttgaggag ttcttcgaaa
     3001 agctgaaaca ggatgcggat gccgattttc ttcggggatt gacctggttg ggattggaaa
     3061 acagtacttt tctgaaaggc aggaaagacc ggccggtact tgaagaatta cgccgaaact
     3121 ttgcaaatac gattttagaa aattattccg taaccgctga ttccgatatt ttggatgatc
     3181 tcaaagtagc ccattttcag cgggttttgg gaaaacaacc ggtaatgtcc gaaacggtag
     3241 caaacgtctt ggatcaattg gaatttgatg aatctttaac gacggaacaa ataatcgaaa
     3301 agttaaacgc tgttattttg aattattttc aatttcaaaa agccgaatat gagaaaaacc
     3361 gattggcttt cctgcaaaag aataaaactg tctatcaatg gaatgaaaca aatcgaaaaa
     3421 gatttctaag gattcccttt ttacggaatc cgaagaacga gccgttggaa ttccccttag
     3481 ccacgaattc cgatcgcgtt ccggtgagaa aaaatatact gacttcctac tggcgcaaat
     3541 accgaaatgc caacgagata aatcaggaaa agtatatccg tgaatattac ggcaaacctg
     3601 tactctcgga ctctgaggtg aaaattctcg aaaaaaagct ttgtaccgga gcgcataaaa
     3661 actgtcgcct gcattttacc cgcggtgaaa ttgatgtttc cgggaatctg tcggaacaac
     3721 aatccgttat tctgatgcag cgggagaaaa acagggctta ttttcggaag aatttggctg
     3781 tcaataatac cggtattatt aaattgacgg aaaaaattcg aaattccatg ctgttgatgc
     3841 cttcctcctt ttcgggtaga gcgaatgccg gaaggttgac gcccgaacgg gcatggagaa
     3901 atttgtacat tcacgataaa aatgtttttc agaaaaaaat tcaaaacgaa atcggtgatt
     3961 tgtcggtgga tattttattg gatgcttccg cttcacagct cggtcggcag gaggcaatag
     4021 cgacccaggg atatatcatt gccgaaagtt tgacacgctg ccaaattcct gttcgggttt
     4081 attcgttttg tacgaaaaga aaatttacga tcatgactct tttcagagat tatgatgaaa
     4141 tttatgataa cgataagatt tttaattact tttcttccgg ttgtaatcgg gatgggctgg
     4201 ctatccggac agcgattcat atgatgaaaa attcacccta tcaacacaaa ttgttgatca
     4261 ttttgtcgga tgcaaaacca ctcgacacga acattgtccc gacaacggga aaaactcagg
     4321 tcgaagcgga ttatacggat ttagtcgggc tgaatgatac ggcggcggaa gtgcggaaga
     4381 gcagagattt cggaatttcc ataatgtgcg tatttaccgg aatggatgag gacctgcctt
     4441 ccgcaaaaag aatctacgga cataatttcg tacgaatcaa agggcttgaa cgatttgccg
     4501 acagtgtcgg aattttgatt caaaatgaat tgaagtggtt gtaaaaatat ttatgcggct
     4561 tttttcttat tcattttaag gaactgaatt ctctccgttt tgaaatccgt tatcgataaa
     4621 cagttgtcta ttcaaaaaat cgtatttgtc tttacaatat aaatatatca acagcgatcg
     4681 tttgatagtg caatgagctg tcaatcaaag gagattgaaa tggaaaagag gagactcgga
     4741 agaacggaga tgatgatcag cccggttgta tatgggggaa tcattcatat gggagagact
     4801 caggaaaccg ccaaccgtta tgtaagttat gctgtcgacc gcggagtgaa ttattttgat
     4861 gtggcacctt cttacgatga tgcggaacaa ttgttgggag tggcgctgaa accttatcga
     4921 aaaaatgttt ttctcgcatg taagacgatg gaacgtaccg catccggatc gctcagagag
     4981 ttggagaatt ctttaaaaac ccttcaaacc gatcattttg atgtctacca acttcattcc
     5041 ataaccactg cggaagacgt cgataccatt ttcggcccgg atggggcgat ggaaacattc
     5101 ttgtcggcaa aaaagaaagg cattatccta aacattggtt tttcggctca cagcgaagaa
     5161 caagcattaa gggcgatgga tttatttgat tttgataccg tgttgtttcc tatgaactgg
     5221 gctttgggat tggttcgcgg atggggagac cgtgtggctc aaaaagttcg cgaaagtgat
     5281 aaagggttgc tctgcattaa gacgctggtc gaccggaaat ggcgagaagg agagaaccgt
     5341 gacgaattcc caaaatcatg gtgtcatccg acaaccgata acgaaaagac cggaatggcg
     5401 ggaatgaaat atgcattctc taagggagcg actgctcttg tccctcccgg tgattttaaa
     5461 agttttaatt tcatgctcga tcatatcgat caggtattgc aaaatccttt gaacgaaacg
     5521 gattggatat atttgcaaga agaagcgcaa aaaattaaag atgaacttat ttttgacaat
     5581 caataaataa aggaagaaat gatgaaccaa acaattaccc atcaattgaa ccatcgctcc
     5641 attcgcgaat ttactgacga accggtgaag ccggaggact taaagctcgt tttggaagct
     5701 gccaaccata cagctaccag taccggtatg caatcagccg ctatgataag agtgacggat
     5761 atggcgaaga aacaggcatt atccgaaata tgtcaacaac cgtatgtcgc gagattcccc
     5821 gaattgatta ttttcatcgt cgatgtctat cgaaatgcac ggatcgcaga agacaacggt
     5881 tatcaatttg aatccgaacg agatatggat cgcttctttc aaggttggac agatgcttgt
     5941 ttaatggcac aaaatgcgac gaatgcgatt gagtctctcg gaatgggcgc tgtttttctg
     6001 ggcagtatct tgaatgattc ggccgcaacg attcaggtgc taaagttgcc gaagttaact
     6061 ttcccggtat tgggcgttgg attcggatgg ccgaaccaga atccgcaatt aaaaccacgc
     6121 ctgccgcttg atttaagagt tttcgaaaat gaatacaaaa ttctggcaga ttataaagaa
     6181 gccgttcggg aatatgacga agagatgcaa acttattatg atttacggga agccaatcga
     6241 cgggtggaca gcttcacttt gcaagtcatt aagaaattgc agaatccgtc ggagaaacga
     6301 gcaaaaatcc ttcaggttgt ccgggatcaa ggttttgatt tgaaagtgtg atatccggaa
     6361 agaatgattt cataagagaa gaaaagaacc gatttaatac gccggatgaa atcatttttg
     6421 gggaaatgct gattaaaaag agcatttcca aaaacaatca atatcctggt gaaaaccgat
     6481 aacaacaagg ggatttgact cttgaatggt ggttttcacc aggttcttat cctctttatt
     6541 caattacgag atacatttgt cccacaatca cgcattgcag aattcttcgg ttctacctcc
     6601 tcgaatacac atcaccaaat tggcactggc aaacaacaca tgaaaccgat tcatattctt
     6661 tgctattccg cgataggcca ctttaaaata gccaaattgc ttcttcacga taagaaacgg
     6721 gtgctcaacc tttgctcgta ctgatgactt ccggctttca atatatttgt cccagttgat
     6781 tcccaatgtt ccatcttgaa gtttgatact tgaaggacgc cgattgattc gaaaatcaat
     6841 agcagaaaga tgcggatcgt tttttatttc ctcatgttgg gcaactccga gataacctga
     6901 atcgccatac acaacggtgt catcctcacg aataagcttt gctgtttctg ccgaatcatg
     6961 cacatttgcg gaagtgccgg ttattgtatg aacataacct gttcctgcgt caaccccaca
     7021 atggactttc atgccaaaat accattggtt cccttttttg gtttgatgca tttcagggtc
     7081 tcgtttcccg tcttcattct tggttgagct tggagctgat atcaaagtcg catcaacaac
     7141 ggttcctcca tgcatgataa gtccggcatt atcaagctga tctttcacat cttcgaagat
     7201 tttttcgcct atcttttctt tttcaagcaa atgtctgaat ttgagaagtg ttgttgcgtc
     7261 aggcacttgt tctgtgaaaa agttgatatg cataaactgc ctcattgcaa aactatcgta
     7321 aatactgtct tctatccctt catctgaaag agagaaccat acctgcaaca ggtactcctc
     7381 agcattgttt ctatccctct cggcggtcgc ccacgtttgc ccttcggata atatggctcg
     7441 atcaatgaca cccatttttt ccatggaatg atctcatcca ttgatttcag aaactcttcc
     7501 cgtttggtaa ttttctttcg ctttgagtac tctacaacac tgaatgattc ttgtttatcc
     7561 atgtgtgatc cgcctctctt tttatgtcat aattatatat tttaattagt gttattagcg
     7621 gtctcggatg cattaaatta gcgtttccct aaaattatca aaatttaact tcattgtaag
     7681 gcgataaaca cccttattca tatgctatct gccgctgatt ttctaattta atcaataggt
     7741 ttttccatta tataatatat gtgaagagga attgccatta agtttcagta gaca
//
LOCUS       DAOMIV010000034         7311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_10221_length_7311_cov_5.809123, whole genome shotgun sequence.
ACCESSION   DAOMIV010000034 DAOMIV010000000
VERSION     DAOMIV010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10221_length_7311_cov_5.809123"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /locus_tag="PKV59_01555"
     CDS             <1..1487
                     /locus_tag="PKV59_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45467.1"
                     /translation="NPVSAPLSPYTAALIRRLRGDLLGIQWEYKQNLDCGDALNQSKD
                     FLYTCNFNFDRLPSANFESTVQLEVRPTGIEKLLEIQLLSAASGQPQNSFPFLPIEKI
                     PAELTVGPIYSPESPDIPLTSVPVGTNAQIDIGFNENSQPFSIENLIVTLEGTPLETC
                     SMNGNNSVRCTLPTSCSDLSENQTCERDLTITACYQGDEIHESSQPVSISYRIVRYPI
                     NLEPSKNTVFQSDLLQHIEQYNEENGNWLPSSITIGNTTFDSYLVRETLSLSGQSEPA
                     AYPFFVNYSLNGFQSAQPLPENFWLDLTVGKSSGGGITEEIISIPPTSVNPNESEILF
                     SLDFGSNLQNTNGYQPGRQFDEVFEIRKIEVRYAGDTLIAPVSVSYQPEGFEFPIKLI
                     SMFEIRLSFQAESLDFGGTTPVPGIDQYTIFCSQPAAPLTCAADPTVPETGCWGNVTL
                     NLSMNPQVAAAPVFAPRCYLGGIRIDNGFDQIWLGDSLNQTFYP"
     gene            complement(1478..2503)
                     /locus_tag="PKV59_01560"
     CDS             complement(1478..2503)
                     /locus_tag="PKV59_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP45468.1"
                     /translation="MEKLKIGIAGLGRLGKIHAKNLADRISGCELTAACSLLESELEY
                     ARTELGVRDCYTNFREMLKNADMDAVAIVTTSGEHCNQLTAALNAGKHVFCEKPLGIS
                     VEECYLAEKAVENHPDKVFMLGFMRRFDPSYIYAKQKIESGTIGEPYLVKATGIDPLK
                     FIEGSLRFAGTSGGLFIDMAIHDIDLMRWYLGAEPKSVYAIGGSYGFPQFAALGDAEA
                     GCALFRFENGAMGTIHSGRTAAHGYHIETEIVGTKGAIRISPIPQKNLAMLYCEQGVV
                     TECVEAFPERFAEAYLSEMQEFVNCIREGRKPAVTVYDGTKATQIAFATTESFKNGRL
                     VEIPPHG"
     gene            2857..3804
                     /locus_tag="PKV59_01565"
     CDS             2857..3804
                     /locus_tag="PKV59_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQP45469.1"
                     /translation="MKKFVLMFLIVIAATGVLVSAVGAEETYKIGYACNNFNDTFQTV
                     IEGYAKAYAKENGVEYVYSDAGEDILKQQDQIKAFIAEGVDALIVVPVDTSAAEPIID
                     MATEAKIPLVFVNRNPFAGVKPEDIPEGVYYVGSESIDAGIFQAEYLVKLLGTEKPVG
                     YAILVGMLSNEAAVARTEGNKQVLSKYPNYKLLAEKNGDWQRDQGLTITENWLTAFGD
                     ELNAILSNNDEMALGALEALEANGRDDVIVMGVDLIDDAKTAILEGRLDASVLQDGKG
                     QGEGAMKIALAALKGEKVDPITMIPYVLVTPENIKDYVE"
     gene            3910..5454
                     /locus_tag="PKV59_01570"
     CDS             3910..5454
                     /locus_tag="PKV59_01570"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQP45470.1"
                     /translation="MDNDNYLLRMVNIDKSFPGVQALKSVNLNVGYGEVHALVGENGA
                     GKSTLMKCLIGIYSPNGGEIYFDGKKIENYTTAQALQMGISMIHQELSPILYRPIMEN
                     LWLGREPVNKFGFVDHNQMYKQSLDVLKEIELDEDPRTLMANLTVAKMQMVEIAKAIS
                     YNAKLIIMDEPSSALTEHEEEQLFKIIRKLKESGTSVIYISHRLSEIISIADKVTVMR
                     DGRDVGTENIENISLDQIITMMIGRTMDEMYPKLKVNIGEPILQVKNLSHRKFFKNVS
                     FEVRRGEIFGIAGLIGAGRTEVIETIFGIRPKSEGEILINGKPVEINSPRDAIANRMG
                     FLTEDRRATGIFSVLGVDVNMAIPNYDRFVTKFGFVNEKAVSESCMKFVNEIQIKTPS
                     LTQKIQNLSGGNQQKVLMARWLMTEPDILFLDEPTRGIDVGAKAEIYKLITMLAAEGK
                     CIVMVSSELPEILGMSDRIMVMHEGRVTAILDNGPDITQEVIMRYATGTENDFKEEYR
                     GIYAKE"
     gene            5441..6427
                     /locus_tag="PKV59_01575"
     CDS             5441..6427
                     /locus_tag="PKV59_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45471.1"
                     /translation="MQKNKFSFQNIFEKYGIVFVLILMIVLMSIAKPSFRTMNNFLNI
                     LTQSSIYGILALGMTLVIISKGIDLSVGSILGLSAVIAASLGQSMTASNKYFPDMGEL
                     PIIIPVIAALLVGSLCGAINGGLIAFTGIPAFIATLGMTTIARGFAYIYTDGKPISTL
                     TPAFKFIGQGKILGIPMPAVIYLIMIIITWVLLNNTRFGKRVYAVGGNINAAEVSGVN
                     VKKMLFLIYTYMGLLAGISAIVSTGRMVSAQPGLAAGYELTAIASTTIGGTSHSGGIG
                     TVWGAVVGALVLAVMRNGMTILGIHSYWQQIVEGLVIIIAVILDMRKNARKK"
     gene            complement(6492..7163)
                     /locus_tag="PKV59_01580"
     CDS             complement(6492..7163)
                     /locus_tag="PKV59_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45472.1"
                     /translation="MKKIIQSYFFRLLRLFLGLFLYALGTVITIQANVGLAPWEAFHM
                     GLSQKTGISFGKIVILVGLLIVMVSVILKEKIGFGTVLNMIFIGTFCDLIIDWNFIPR
                     IDNYGLGILFMLFGLFVICLGSYFYIGSALGAGPRDSLMIGLKKKFSGIPVGMIRGMI
                     EGTVLLLGWLMGAKVGLGTVIAVFGISFILQFTFKCLRFDVDDLHSESVTETVRNLGV
                     IRKES"
BASE COUNT         2084 a         1630 c         1598 g         1999 t
ORIGIN      
        1 taaatcctgt ttctgcccct ctttcccctt ataccgccgc attgatcaga cggttgcggg
       61 gagatctttt ggggattcaa tgggaataca aacagaattt ggattgcggc gacgctttga
      121 atcaaagcaa ggattttcta tatacctgta atttcaattt tgatcggctg ccatcggcaa
      181 actttgaatc aacggttcaa ctggaggtga gaccgacggg aatcgaaaaa ttgctggaaa
      241 tccaattgct gtccgcagcc agcggtcaac cgcagaattc tttcccattt ttaccgatcg
      301 aaaaaatccc cgccgaactg acagtcggtc ccatttattc gcccgaatct cccgatattc
      361 ctttaacatc ggtccccgtc gggacaaatg cacagatcga tatcggtttc aacgaaaatt
      421 cacagccatt ttctatcgaa aatctgatcg tgacgctcga aggcactccc ttggagactt
      481 gttcgatgaa cgggaacaat tccgttcgtt gtacattgcc gacgagctgt tctgatctct
      541 cggaaaatca aacctgtgaa cgagatttaa cgatcactgc ctgttatcaa ggggatgaaa
      601 tccatgaaag cagccaaccg gtcagtattt cttatcgaat cgtccgttat ccgatcaatc
      661 ttgaaccttc gaagaatacc gtttttcaat ccgacctctt gcagcatatt gaacaatata
      721 atgaagagaa tgggaattgg cttccgtcat cgattacgat tggaaatacc acatttgatt
      781 cgtatttggt tcgggagacc ctttcacttt ccgggcagtc cgagccggcc gcttatccgt
      841 tttttgtcaa ttactcatta aacgggtttc aatccgctca acctcttccg gaaaattttt
      901 ggttggatct gaccgtcgga aaatcttccg gcggaggaat cacggaagaa atcatttcta
      961 ttccgccgac atcggtcaat ccgaatgaat ccgaaatttt attctcactc gatttcggct
     1021 cgaaccttca gaacacgaac ggttatcaac cgggtcgaca attcgatgag gtatttgaaa
     1081 ttcgtaaaat cgaagttcga tatgccggag atactttgat cgctccggtc tccgtttcct
     1141 atcaaccgga aggattcgag ttcccgatca aattgatttc gatgtttgaa atccgactca
     1201 gtttccaagc cgaatccctt gatttcggcg gaacaacacc ggtccccgga atcgatcaat
     1261 atactatctt ctgttctcag ccggcggctc cattaacctg tgccgccgat ccgaccgttc
     1321 cggaaacggg ctgttgggga aatgttacgc tcaatctttc gatgaatccg caagtggcag
     1381 ccgcaccggt ttttgcaccg cgctgttatt tgggaggaat tcgtatcgat aacggattcg
     1441 accaaatctg gctgggagat tcactgaatc aaacctttta tccgtgagga gggatttcaa
     1501 ccaaccgccc gtttttaaag gattctgtcg ttgcgaaagc aatctgagtg gctttggttc
     1561 cgtcatagac agtaaccgcc ggtttacgtc cttctcggat acaatttacg aattcctgca
     1621 tttccgaaag atatgcttcc gcaaatcgtt ccggaaaggc ttcaacacat tcggtgacta
     1681 ctccttgctc gcagtatagc atcgccagat ttttttgcgg aatcgggctg atgcgaatcg
     1741 cgcctttggt cccgacaatt tcggtctcga tatgatagcc atgcgctgcc gttcggccgc
     1801 tgtgaatggt tcccatcgcc ccgttttcga accggaacaa ggcgcaaccc gcttccgcat
     1861 cccccaacgc agcaaattgc ggaaaaccat agctgcctcc gatggcatat acgcttttgg
     1921 gttctgcacc caaataccag cgcattaaat cgatatcatg gatagccata tcgatgaata
     1981 agccgccgct cgtcccggca aaacggaggg aaccttcgat aaatttcaga ggatcaatac
     2041 cggtggcttt gaccaagtag ggttcgccga ttgtaccgga ttcgattttt tgttttgcat
     2101 aaatataaga aggatcaaag cgtctcataa atcccaacat aaagacctta tcgggatgat
     2161 tttcaaccgc tttttccgcc aaatagcatt cctcaacgga aattcccaac ggtttttcac
     2221 agaaaacgtg cttaccggca ttcaacgccg ccgttaattg gttgcaatgt tcgcctgagg
     2281 tggttacgat ggcaacggca tccatatcgg catttttcaa catctcccga aaatttgtat
     2341 aacagtccct gacgccgagt tcggtacggg catattccaa ctcgctttcc aacaggctgc
     2401 aagcggctgt caattcgcaa ccggaaatcc gatcagccaa gttcttggcg tgaattttgc
     2461 ctaatcgccc caagcccgca ataccgatct ttaacttttc cataaggttc tccttcgttg
     2521 tctcaaactt ttttttatcg aatgtgattc caaagccaaa cggttatttg ttgaaaaata
     2581 ttgaaagtgt tcagtccttt attgcgatta taactttcag cccaaagtca gaaagtgaaa
     2641 ggaactcttt ttcatcataa aaccaaagaa ttaagaaatt tgtcacgtcg tatatcatat
     2701 taaatattat ttttataacc tattagtttt tctgtttatc ttgatataat cataaaatgt
     2761 aaacgtttac gttaacccat atttgtgttt tcagcttaga attgaattta cttttagccg
     2821 ttctcaacaa tttagtttga accataggag ggaaaaatga agaaatttgt attgatgttc
     2881 ctgattgtta ttgcagcaac aggtgtgctc gtcagcgcag tcggtgcaga agaaacctat
     2941 aaaatcggtt atgcctgcaa caattttaat gacacgtttc agacagtcat tgaaggttat
     3001 gcaaaagcct atgcaaagga aaacggtgtt gaatacgttt attccgatgc cggagaagat
     3061 attttaaaac agcaagacca aattaaagct ttcattgccg aaggtgtcga tgctttaatt
     3121 gtggtgccgg tcgataccag cgctgccgaa ccgatcattg atatggccac cgaagcaaaa
     3181 attccgctgg ttttcgtcaa tcgcaatcct tttgccggcg tgaaacccga agatattccg
     3241 gaaggagtct attatgtggg ttcggaatcc atcgatgccg gaattttcca ggctgaatat
     3301 ttggtaaaac tgctgggaac cgaaaagccg gtcggttatg ccatcctcgt cggaatgctc
     3361 tccaacgaag ccgcagtagc ccgaaccgaa gggaataaac aggttttaag caaatatccg
     3421 aattacaaat tattggctga aaagaacggt gactggcaac gtgatcaagg gttgaccatc
     3481 accgaaaact ggctcactgc ctttggcgat gaattaaatg caatcctttc aaataatgac
     3541 gagatggcat taggtgcgct ggaagcgttg gaagcgaacg gccgggatga tgttatcgtg
     3601 atgggtgtcg atttaatcga cgatgcaaag accgccatct tggaaggacg tctcgatgct
     3661 tctgtgcttc aagacggtaa aggtcaaggt gaaggcgcga tgaaaatcgc tttggcagct
     3721 ctgaagggtg agaaagttga cccgattacc atgatcccgt atgttctggt gacaccggaa
     3781 aacattaagg attatgttga ataaatcgat tcattagctg taaacaggga aaagagccgt
     3841 acttttccct gtttatttct caatctgtaa atcagtttgt tttaaattcc aaaaaatgag
     3901 gggaaaacaa tggataatga caattacctg ttacgtatgg taaacattga taagtcattc
     3961 cccggggtac aagcgctgaa aagcgtcaat ttgaacgtcg gttatggcga agttcatgcg
     4021 ctggtgggtg aaaacggcgc aggaaaatca actttgatga aatgcctgat cgggatttat
     4081 tcgccaaacg gaggcgaaat ctatttcgac ggtaaaaaaa ttgaaaatta cactacagct
     4141 caagcgttac agatgggaat ttccatgatt catcaggaat tatctccgat cctttatcgc
     4201 cctatcatgg aaaacctatg gctcggccga gaaccggtca ataaattcgg ctttgtcgac
     4261 cataatcaaa tgtacaaaca aagcttggat gtgctgaaag aaattgagct ggatgaagat
     4321 ccgcggacgt tgatggcaaa tctgaccgtt gcgaaaatgc aaatggtgga aatcgcgaaa
     4381 gccatctctt acaatgccaa attgatcata atggatgaac cctcttcagc tttgacggag
     4441 cacgaagaag aacaactatt taagattatt cgcaagctga aggaatccgg cacatccgtc
     4501 atttacatat cacatcgact gtccgaaatc atctcaattg cggataaggt caccgtgatg
     4561 cgtgacggac gggatgtcgg aaccgaaaac atcgaaaaca tttctcttga ccaaatcata
     4621 acgatgatga tcggtcggac gatggatgaa atgtatccga aattaaaagt aaatatcggc
     4681 gagccgattc tgcaagtcaa aaacttatct catagaaagt tctttaaaaa tgtctctttt
     4741 gaagtccggc gcggcgaaat ttttggaatt gcggggttga tcggcgccgg tcgaacggag
     4801 gttatcgaaa ctattttcgg cattcgtccg aagtccgaag gtgaaattct cattaatggg
     4861 aaacccgttg aaatcaattc accgcgggat gcaatagcca accgcatggg gttcctgacg
     4921 gaagatcggc gtgctaccgg tatcttttcc gtattgggcg tggatgtcaa tatggcaatt
     4981 cctaattatg accgttttgt cacaaaattc ggatttgtaa acgaaaaagc cgtctctgaa
     5041 agttgtatga aatttgtcaa cgaaatccag ataaaaacac cgagcctcac tcaaaaaatt
     5101 caaaatctca gcggaggaaa tcagcagaaa gttttgatgg ctcgttggct gatgaccgaa
     5161 ccggatattc tgtttcttga tgaacccacc cgcggaattg acgtcggtgc aaaagcggaa
     5221 atatacaagt tgattaccat gcttgcggcg gaagggaaat gcatcgtcat ggtctcatcg
     5281 gaacttcctg aaattttagg catgagtgat cgaattatgg tcatgcatga agggagggtt
     5341 acagccattt tggacaatgg acccgatatt acgcaagaag taatcatgcg ttatgcaacc
     5401 ggtacggaaa acgattttaa agaagaatat agaggaatct atgcaaaaga ataaattttc
     5461 ttttcaaaac atattcgaga aatacggaat tgtttttgtt ttgattctga tgattgtatt
     5521 aatgagcatc gcaaaaccca gtttccgcac catgaacaat ttcctaaaca ttctgaccca
     5581 aagctcaatc tatgggatcc tcgctctggg aatgacattg gttatcatct cgaaagggat
     5641 tgatctttcg gtcggatcga ttttaggatt atcggctgta atcgcggctt ctttaggaca
     5701 aagcatgaca gcttctaaca agtatttccc cgatatggga gaactcccga ttattatccc
     5761 ggtaatcgca gcgttgttgg tcggcagttt gtgcggcgca atcaacggcg gtttaattgc
     5821 tttcaccgga attcccgctt tcatcgctac attaggcatg acgactattg cgcgaggatt
     5881 tgcctatatt tacacggatg gaaaaccgat cagtacatta acccccgctt tcaaatttat
     5941 cggccagggg aaaattttgg gcattcctat gccagcggtt atatatctga tcatgattat
     6001 catcacctgg gtgctcttaa ataacacacg attcgggaag cgggtatatg ctgtcggcgg
     6061 aaatatcaac gcagccgaag tctcgggtgt caacgtcaaa aagatgctct tcctgattta
     6121 cacttatatg ggattgttgg ccggcatttc ggccattgtc tcaaccggaa gaatggtttc
     6181 cgcacaaccc ggactggctg ccggctatga attgactgca atcgcttcca ctacgatcgg
     6241 aggaaccagt cattccggcg gaatcggcac ggtttggggc gctgtggtcg gcgcactggt
     6301 gctcgcggtc atgagaaacg gaatgactat tttaggcatc cattcttatt ggcagcaaat
     6361 tgtcgaaggt ttggtgatta ttattgcggt tatccttgat atgcgtaaaa atgcccggaa
     6421 aaaatagaat ctcctttttt tatttccgaa cggccgcagg ttttgattct gcggccgttc
     6481 aattttacct atcatgattc ctttcgaatt accccgaggt tacgaaccgt ctcggtaaca
     6541 ctttcgctat gcaaatcatc tacatcaaaa cggagacact taaaggtaaa ttgaaggata
     6601 aagctgattc cgaagacagc gataacggtt ccgaggccca ctttggctcc catcagccaa
     6661 cccaataaca gaaccgttcc ttcgatcata ccgcggatca tccctaccgg aataccgctg
     6721 aacttctttt ttaatccgat catcagagag tctcgcggac cggcgcccaa agccgaaccg
     6781 atataaaaat aggacccgag acagattaca aataagccga acaacatgaa aaggatcccc
     6841 aagccatagt tatcgattcg cggaataaaa ttccagtcga taatcagatc gcaaaaagtt
     6901 ccgataaaaa tcatattcaa tacggttccg aagccgattt tctctttcaa aataacggat
     6961 accataacaa tcaataaacc gaccagaata acgattttcc cgaatgaaat gccggttttt
     7021 tgcgataaac ccatatgaaa agcttcccat ggagctaaac cgacgttggc ttgaatcgta
     7081 attacagttc ccaatgcata aagaaataat cccaggaata gtctcaataa acgaaaaaag
     7141 taagattgaa tgattttttt caatttttat acagtcacat ttcacccgat tttatgactt
     7201 gatgatactg tcacaacgca aaaaaacatg agaacatttt caaatggatg caatattttg
     7261 gatggtatcc aaaacgcttt cacttttcac ttttttcacc acctgcactt t
//
LOCUS       DAOMIV010000035         7156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_10547_length_7156_cov_3.637234, whole genome shotgun sequence.
ACCESSION   DAOMIV010000035 DAOMIV010000000
VERSION     DAOMIV010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10547_length_7156_cov_3.637234"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PKV59_01585"
     CDS             <1..736
                     /locus_tag="PKV59_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQP45473.1"
                     /translation="EDGWAAREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLI
                     RATKKFDYRRGHKFSTYATWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRVQHQLT
                     QKLGRDPSVEELADALDVPPKKVENMIQVARRPLSLETPTDDEEDSVLGDFIEDEDAP
                     APDDTATYNLLKEHLDEVLNGLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERV
                     RQIEAQALSRLRHPTVRKILRDYLGD"
     gene            829..2241
                     /locus_tag="PKV59_01590"
     CDS             829..2241
                     /locus_tag="PKV59_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45474.1"
                     /translation="MAKIELNDYLGRIDTLVSESKYEQAAYHCFYLLRQFPKLLSLYK
                     LLGRIFLEMNELRPALDTYSRIISAEPDNFLNHLALGFIFEELKLPDQAIFFSQAALF
                     LQPENKEVVSLYQRLLSRMKNYPNPESFSHLLAGIHFYNERNYEKAINELQINSLESY
                     QTFGSLFIGLSLFENHQEDEGIKLLQIILEKSPYLQSALQKTAIYWQKKNPQLFMKYL
                     NRLIELNPDYSEYTIQDNQLTCGNNSIQVIYQEWTGFQNSKLRTVWQQPGSHIIENDL
                     STLPSWLKLLPVCPDFLYQTNNRNEEENVFYSSERKKILLEEDSFFQRDYFKSQIPES
                     DKNSQKQRESTEKDQKIGKSSRSTKSLDDAFHWLEKVVTEGVEIKQEGTAEKQFESDR
                     QVDIDEFENDLQNKEETEMVRNAWTCFSTGNQVEGIRLYKQLIEKQIKPEMVQEDLQK
                     LLILFPENEELQKITNERGK"
     gene            2244..2702
                     /gene="ndk"
                     /locus_tag="PKV59_01595"
     CDS             2244..2702
                     /gene="ndk"
                     /locus_tag="PKV59_01595"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQP45475.1"
                     /translation="MEKTFVCIKPDGVQRGLIGEIIQRFENRGLILCAAKFMIVSKEF
                     AERHYAEHIGKPFYNGLVNYITSGPVFAMVWEGEEAVKAVRQTVGATKPIESAPGTIR
                     HDFSAKTDRNLIHASDSVTTAEREINLWFEPDEIVTWDKISSPWVFGNNS"
     gene            complement(2703..3131)
                     /locus_tag="PKV59_01600"
     CDS             complement(2703..3131)
                     /locus_tag="PKV59_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45476.1"
                     /translation="MIPVLNAAKINCDSILPSRKHSDDAGLDLYASETKIIEGNGINV
                     IGTGIAVDIPKGYVGLIFLKSGSNFLLGGGVVDAGYQGEIKIKIFNIKTETIQIEKGQ
                     AIAQLLIVPIETPEVREVQISDLFKEKSDRGAEGGIHLPI"
     gene            3423..4850
                     /locus_tag="PKV59_01605"
     CDS             3423..4850
                     /locus_tag="PKV59_01605"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQP45477.1"
                     /translation="MENTSIPNHILVMKYGGSSVGSPVAMKSAVKIICETKKEYPNLV
                     VVTSAISGATDALIKSATDASSGNLTTAEKTAHELTARHHELIDKLVEDPKIWLQLKM
                     DVNRLISEFVNLCNAIKILGELSPRALDAVSSLGERLSVRILSAAVESAGIKSKPVES
                     TQLIITDDVYQSASPDMELTSVRCKKVLYPILNEGITPIVTGFIGATKEGSITTLGRG
                     GSDYSASIIAVGLEAEEVWIWTDVTGVMSADPRIVPDAKTIPALTYNEVSEMAYYGAK
                     VLHPKSVKPCVENGIKMRICNTFAPQSQGTILIHNNDAVDVGKIKAVTTASGFKLITV
                     SGAGMDSGMNASAKIFNGFVEAGVNVPMVIESSSEQSLCFPIDIKKVNKVEKVLRTIL
                     EKEISRKDIDEFEISEPVDILTVICSGLKSHPEVITQVLSKLIENQYKIKALSYGASD
                     VSLNIIVSSSDSISALKAIHQLISF"
     gene            4873..5934
                     /gene="asd"
                     /locus_tag="PKV59_01610"
     CDS             4873..5934
                     /gene="asd"
                     /locus_tag="PKV59_01610"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQP45478.1"
                     /translation="MSNKIPVSVLAATGSVGQRFISLLDHHPWFEVVEITASDRSVGK
                     TYEESCHWLLSDPMPEYVKKMELLPTDADAIKKSQIAFSALPAEIAMIVEPDFARKGI
                     YVSSNASAYRKDPLVPILVPEVNPDHLQILDVQQKTYQWPGAIMTNPNCTTVGFVVPL
                     NAIDKHFGVTKAIVFSMQALSGAGYPGVPGMDILDNVIPFISGEEEKLEYEPAKIMGN
                     FDGEKIIQHPIVISAHTNRVAVTDAHTICLSFAVREKVSEEQIISTLENFEFPEICRG
                     LPSCPKKGIIYHKEENRPQPRWDRSLEKGMATNVGRVRKDNILDYRMVTVSHNTIRGA
                     AGGSILNGELLAKIKFRMN"
     gene            5949..6965
                     /locus_tag="PKV59_01615"
     CDS             5949..6965
                     /locus_tag="PKV59_01615"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HQP45479.1"
                     /translation="MKNVKIGVVGYGNVGKGFVQALNQKTDFYRKIYQLNFFITAIVT
                     KNNGCVYNPNGISLDELLTVNSFAELSDVERPDWNTISMIQQAETDVIVEISSTNLIN
                     GEPAVLHIREAILSGKNVVTSNKGPIALHYKMLNSLANQNNVKLGIEGTVMSGTPSLR
                     FAKDLLFPSGITSVRGILNGTCNFILTEMEKGLSFNEALAHAQEFGYAETDPSGDIEG
                     LDTAGKVAILSQLIFDEKQTPAEMGYEGITGITVEDIQMAKKENCRWKLIGSVKKVQN
                     HIITSVKPEMVPLSNPLANVNGSMNAIQYETDLLGMVTLIGPGAGRMETAAALLEDLI
                     TIYK"
BASE COUNT         2317 a         1352 c         1493 g         1994 t
ORIGIN      
        1 tgaagatggt tgggctgcca gagagcattt aattaccgca aattcccgct tggttatcag
       61 cgtggccaaa aaatatatgg gaagaggtgt tccttttctc gatctgatac aagaagggaa
      121 tatcggtcta atcagagcta caaagaaatt cgattataga cgagggcata aattcagcac
      181 ttacgcaaca tggtggatcc gtcaggcagt aacacgtgct attgcagatc aaggaagaac
      241 aattcgagtc cccgttcata tgggcgacca aattaacaaa ttactgcgtg tacaacacca
      301 actgacgcaa aaattgggtc gcgatccttc tgtggaagaa ctggcggatg ccttggatgt
      361 tcccccgaag aaagtcgaaa acatgatcca agtagcgaga agaccgctat ctttggagac
      421 acctaccgat gatgaggaag attccgtctt aggcgatttc atcgaagatg aagacgctcc
      481 ggcacctgac gataccgcta cttataattt acttaaagag catttagatg aagtgctgaa
      541 tggtttgcca ccgagagaag ttcgaattct gcaacttcgg tatggtttgt tagatgggca
      601 agcgtacact ttggaagaag tcggacgaaa gatgggcgta acgcgagaac gtgtccgcca
      661 aattgaagct caagcattga gtcgtctgcg acatccaacc gttcgaaaga tcttaagaga
      721 ctatttaggt gattaattaa tccaaacaaa aagccattca gcattgatct tgctgaatgg
      781 cttttttgtt tggatatttc gtttaaaata agaacgaaag gaatttaatt ggctaagata
      841 gaattgaatg attatctcgg gagaattgat acgcttgtgt ctgaaagcaa atatgaacag
      901 gctgcatatc attgttttta tcttctgaga caattcccca aattattatc cctttataaa
      961 ttattgggaa gaatattcct ggaaatgaat gaactcagac ctgctctgga tacttattct
     1021 cggatcattt ctgcagagcc ggataatttt ttaaaccatt tagcactcgg ttttattttc
     1081 gaagaattaa aactgccgga tcaagccata tttttttcgc aagccgcttt atttttgcaa
     1141 ccggaaaata aagaggttgt cagtttatat cagcgattgc tcagccggat gaaaaattat
     1201 cccaatccgg aaagtttttc tcatctttta gcgggaattc acttttataa cgaaagaaac
     1261 tatgaaaaag caatcaatga gctgcaaatt aattcacttg aaagttatca gaccttcgga
     1321 tctttattta tcggactaag tttgtttgaa aatcatcaag aggatgaggg aataaaattg
     1381 ctccaaatca ttttggaaaa atcaccttat ctgcaatcag cattacagaa aaccgccatc
     1441 tattggcaaa aaaagaaccc tcagcttttt atgaaatacc tcaatagatt aattgaatta
     1501 aatcccgatt attccgaata taccatacaa gataatcaat taacctgcgg gaataattcg
     1561 atccaagtga tctatcagga atggacaggt tttcaaaact cgaaattgag aacggtttgg
     1621 caacagcccg gatcacatat tatcgaaaat gatctttcca cattaccttc ttggttaaaa
     1681 ttattgcctg tttgtccgga ttttctttat cagacgaata accggaatga agaagaaaat
     1741 gtattttatt cttcagaaag aaagaaaatt ttattggagg aagattcttt cttccaaaga
     1801 gattatttta aatcccaaat tcccgaatca gataagaatt ctcaaaagca gcgtgagtca
     1861 actgagaagg atcaaaagat aggaaaatca tcccgctcga caaaaagttt agatgatgct
     1921 tttcactggc tggaaaaagt ggtcaccgaa ggggttgaaa taaaacagga aggtactgcc
     1981 gaaaaacaat ttgaatctga ccgccaagtg gatatagatg agtttgaaaa tgatctccaa
     2041 aataaggaag aaacagaaat ggttcgaaat gcatggacat gtttcagcac cggaaatcaa
     2101 gtagaaggta ttcgacttta taaacaatta attgaaaaac aaataaaacc ggaaatggtt
     2161 caagaagatt tgcagaaact tttaattctt tttccggaaa atgaagaact tcaaaaaatc
     2221 acaaatgaaa gagggaaata aaaatggaaa aaacattcgt atgtatcaag cctgatggtg
     2281 ttcaacgtgg attaatcggt gaaattattc aacgatttga aaatcgcggg ctcattcttt
     2341 gtgccgcaaa atttatgata gtttcgaaag aattcgccga acgtcattat gccgaacata
     2401 tcgggaaacc tttttacaac ggtttagtca attacattac atccggtccc gtttttgcaa
     2461 tggtttggga aggtgaagaa gcggtgaaag cggttcgcca gaccgtcgga gctacaaagc
     2521 ctattgaatc ggctcccgga acaattcgcc acgatttcag cgccaaaacg gaccggaatc
     2581 ttatccatgc atccgattcc gttacaacgg ctgagcgtga gattaacctt tggtttgaac
     2641 cggatgaaat agttacctgg gataaaatca gctctccgtg ggttttcgga aataattctt
     2701 aatcagatcg gtaaatggat acctccttcc gctccacgat cggatttttc tttaaataaa
     2761 tcagaaattt gaacttcccg aacctcggga gtctcaatcg ggacaatcaa caattgagcg
     2821 attgcctgtc ctttctcaat ttgaattgtt tcggttttaa tattaaatat cttaattttg
     2881 atttctccct gataaccggc atcaactacc cctcctccca gcagaaaatt gctgccgctt
     2941 ttgagaaaaa taagtccgac ataacctttc ggaatatcaa cggctatgcc ggtaccgatc
     3001 acattaattc cgtttccttc aattattttt gtctcggaag catataaatc aagtccggca
     3061 tcatcggaat gcttccggct gggaagaatc gaatcacaat ttatttttgc tgcattaaga
     3121 acaggtatca tttttcccct ttccgatctt taaatcattt aagcagtctc attatattaa
     3181 acttttttag aaatgttgtc atatcttttt aaaattcatt tgcagaaaac aattgttttc
     3241 gattaaagtt gatgcatata tgaaacacac agatttccga aattttgttg aatatatcgt
     3301 aacgactacg gggactactc tgctctatct accttgaaga ggcggattct ttacgaattc
     3361 aaataaaact aagagctgcc tcaaaagggc agcttttttt atatcgaaat gaggtctttt
     3421 ttatggaaaa cacatcgata ccaaatcata tattggtgat gaaatatggc ggaagctccg
     3481 tcggttctcc tgtagcaatg aaatcggcag tcaaaattat ctgtgaaacg aaaaaagagt
     3541 atcccaatct tgttgtggtt acttctgcca tttcaggagc caccgatgct ctgataaaaa
     3601 gcgcaaccga tgcttcgtca gggaatttaa ccaccgcaga aaaaaccgct catgagctta
     3661 ctgccagaca tcatgaattg attgacaaat tggtcgaaga tccgaaaatc tggcttcaat
     3721 taaaaatgga tgtaaatcgt ctaatatccg aatttgtaaa tttatgcaat gcaatcaaga
     3781 ttctcggaga attaagtccg cgcgctttag atgcggtttc aagtttggga gaacgcttgt
     3841 cggttcggat tttatcggca gcggtcgagt cagccggaat taagtcaaaa ccggtcgaat
     3901 caactcaatt gatcataacc gatgatgttt atcaaagcgc ctctccggat atggaattaa
     3961 cctccgttcg atgcaaaaaa gtcctatatc cgatactcaa tgaaggaata accccgattg
     4021 ttaccggatt tatcggcgca acgaaagaag gatccattac cactttaggg agaggaggaa
     4081 gcgattattc cgcttctatt attgcagtag gattggaagc ggaagaagtt tggatttgga
     4141 cggacgtgac cggtgtcatg agtgccgatc cgcgaattgt tcccgatgca aagacaattc
     4201 ctgcattaac ctataatgaa gtttcggaaa tggcatatta cggagcgaaa gtactccatc
     4261 ccaagagcgt caaaccttgt gtggaaaatg gcataaagat gcgaatttgc aatacgtttg
     4321 caccacagag tcaagggact atcttgattc acaataatga cgccgtggat gtcggtaaga
     4381 ttaaagcggt tacaacggca tcgggattta aattgataac tgtttcgggt gcaggaatgg
     4441 attccggaat gaatgcatca gccaaaatat ttaatggatt tgtcgaagcc ggagtaaatg
     4501 tcccgatggt gattgaaagt tcttcagagc aatcattgtg ttttccgatc gatataaaaa
     4561 aggtgaataa agttgaaaaa gtactcagaa ccattctgga gaaagaaatt tcgcgaaaag
     4621 atattgatga atttgaaatc agcgaaccgg ttgatatctt aaccgtgatt tgttccggac
     4681 ttaaatccca tccggaagta atcacgcagg tattaagtaa attaatcgag aatcaataca
     4741 aaatcaaagc tctttcttac ggagcctcag acgtatcatt gaatattatt gtcagttcct
     4801 ccgattcgat ttcagctctc aaggcgattc accaattgat ttcattctaa tcacatgaaa
     4861 ggatataaga ttatgagtaa taaaattccc gtctcagttt tagctgcaac cggaagtgtc
     4921 ggtcaacgtt ttatcagttt attggatcat catccgtggt ttgaagtggt cgagataaca
     4981 gcttcggatc gatcggtcgg aaaaacttat gaagaatcct gtcactggct tctttcggat
     5041 ccgatgcccg aatacgtaaa aaaaatggag ctgctaccta ccgatgcgga cgcgatcaag
     5101 aaatctcaaa tagctttctc ggcactcccg gccgaaattg ccatgatagt cgaacccgat
     5161 tttgccagaa aaggcatata tgtcagttca aatgcttctg cctatcggaa agatccgctg
     5221 gtaccgattc tggttccgga ggtaaatccc gatcatctac aaatattaga tgttcaacag
     5281 aaaacatatc aatggccggg agcgattatg acgaatccga actgtacgac agtcggcttt
     5341 gttgtcccat taaatgcaat cgacaaacat tttggcgtta caaaagcaat tgttttttcg
     5401 atgcaagctc tttccggagc aggctacccc ggcgtacccg gtatggatat cttggataac
     5461 gtcatccctt ttatcagcgg agaagaagaa aaattggagt atgaaccggc caaaattatg
     5521 ggaaattttg acggtgaaaa aatcatccaa catccgattg taatttcggc tcatacgaat
     5581 cgggtagcag ttacagatgc gcacacgatt tgcctgtcat tcgcggttcg agaaaaggtc
     5641 tctgaagaac agattatatc aactttggaa aattttgaat ttccggaaat atgccgaggt
     5701 cttccatcct gtcctaaaaa aggtatcatt tatcataaag aagaaaatcg tcctcaaccg
     5761 cgttgggatc gttcactgga aaaaggaatg gctaccaatg tcggaagagt tcgaaaagat
     5821 aatatcctgg attatcgaat ggttacggtt tctcataata caatcagagg agcggcaggc
     5881 ggatcaattc taaatggaga attactggca aaaataaaat tcagaatgaa ctaaaaagga
     5941 gcccactcat gaaaaatgtc aaaatcggag tcgttggcta tggcaatgtc ggaaaaggtt
     6001 ttgtccaggc attaaatcag aaaacggatt tctaccgtaa aatttatcaa ctaaattttt
     6061 tcattacagc aattgtcacg aaaaataacg gttgtgtcta taaccctaac ggcatttcat
     6121 tggacgaatt gcttacggta aattcattcg ccgaattaag tgatgttgaa cgaccggatt
     6181 ggaacacaat ttcaatgatt caacaagctg aaaccgatgt aattgtggaa atcagttcga
     6241 cgaatttaat caatggagaa ccggcggttt tgcatattcg agaagcgatt ctctccggta
     6301 agaatgtggt taccagcaac aaagggccga ttgctttgca ttataaaatg ctgaacagtt
     6361 tagccaatca aaacaatgtg aaactgggga ttgaaggaac agtaatgagc ggaactcctt
     6421 ctctgaggtt cgcaaaagac ttgctttttc cttccggcat cacttccgtg cgaggcattc
     6481 ttaacggaac ctgcaatttt attttaacgg agatggaaaa aggattaagt tttaatgaag
     6541 ctcttgccca tgcacaagaa ttcggttacg cggaaaccga tccgagcgga gatatcgaag
     6601 ggttagacac agctggaaaa gttgcgatcc tttcacaact gatatttgac gaaaaacaga
     6661 caccggctga aatgggttat gaagggatta ccggaataac cgtcgaagat atccaaatgg
     6721 caaaaaaaga gaattgcaga tggaaattga tcggttcagt taagaaagtt cagaaccata
     6781 taataacttc tgtaaaaccg gaaatggttc cattatccaa tccgttggca aatgttaatg
     6841 gctcgatgaa tgcgattcaa tatgagacgg atttgttagg tatggttaca ttaatcggac
     6901 ccggggcagg tcgtatggaa actgccgctg ccttattgga agatttgata accatttaca
     6961 aatagattta tttttccgtt tgatttcttg agtataccaa taaaaattca tagcctcttt
     7021 tataatcaat tggaggctat gaaatattat tcaaaaaata taaacccatt cggatcggtc
     7081 aaataaaagc ggaaatgatt ggtaaatcca atcttgggag ccaataagaa tacaaccgat
     7141 tagcaacccg aatata
//
LOCUS       DAOMIV010000036         7129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_10609_length_7129_cov_4.053152, whole genome shotgun sequence.
ACCESSION   DAOMIV010000036 DAOMIV010000000
VERSION     DAOMIV010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10609_length_7129_cov_4.053152"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PKV59_01620"
     CDS             complement(<1..514)
                     /locus_tag="PKV59_01620"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQP45480.1"
                     /translation="MIRKMVEIDEEKCTGCGLCAEACRENAIQMVNGKAKLIRDDYCD
                     GLGNCLPVCPANAITIIEREAIPFDKEAVRHQATVNNMTNVPSGCPGKQAKTLRRDTS
                     NPIIESSRESQTSELMQWPVQLKLVPVNAPFFDNCNLLIAADCTAFAYNNFHRDFIKK
                     HVTLIGCPKLD"
     gene            605..1303
                     /locus_tag="PKV59_01625"
     CDS             605..1303
                     /locus_tag="PKV59_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQP45481.1"
                     /translation="MKSYDPLLLQMPLFYNFSPKDLDSLRNCLSFRVRKYLKGEAIFL
                     EGDEISRLGIILNGSVIIVKEDISGNRNIISTFSTGNLFGEAFAFSKQKRIMVSVFSE
                     SDSEILFLDAEHLLTLCKELCPFHSQLIRNLMNEFAEKTILLNEKIDLLSQKTIQDKV
                     LAFLFSEAKRQSSRSVQIPFNRQQLADYLFVDRSALSAELSRMQKKGILEYHKNKFVL
                     KTIAHFDDNSSFNL"
     gene            1359..1949
                     /locus_tag="PKV59_01630"
     CDS             1359..1949
                     /locus_tag="PKV59_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45482.1"
                     /translation="MEQKLSFCTSEILIRNMRSPLPYGLTVIQGPHAASGLLLQICAK
                     LALRGPVRILDCGNRSDMYQVARELRPLTRDPATAMQRIHLSRAFTCYQAEALLGQNR
                     GQSQTPILIFDLLATYLDESVSKQEAERLFDDTLDHLQKMSRKNPVLVGIRPLPAIAL
                     DRSNLPLRLQKAADESFQLMEISQKAETNDDQLSLF"
     gene            1971..2318
                     /locus_tag="PKV59_01635"
     CDS             1971..2318
                     /locus_tag="PKV59_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45483.1"
                     /translation="MGRTIRSATQTWIEEEKALGRFARALRKEDQRAMMELINLSRLH
                     IAESSYASNLYPMDVYLISMLLEMFKRVQRDEATLAEVCKHAGIELPSVSTEIPEFPD
                     LVDLIESLDQADA"
     gene            2330..4546
                     /locus_tag="PKV59_01640"
     CDS             2330..4546
                     /locus_tag="PKV59_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HQP45484.1"
                     /translation="MQMHARGWLLDLYEDANDGVRLWFILENDQRICLKQQLPITFYI
                     SGDSKRLHQCCLFLKHLPGMIAMQRVSKKDVFKTEPVCALQVDMADPVHQRQAFLKLK
                     KDFPDLTYYNADLAVHIHHAALYQTFPMAFCDLLYDSESGLIQMLKVLNSRWDITPLH
                     PVLRILEIAPDCDPIRKKPEMLKICDRRGHKQTICLREPTLLLEHLNALIAAIDPDIV
                     ETQWGDDWLFPLLLQWEQETGIPLNFNRDPERKLRWKKEITYFSYGQIVYRGREAHLF
                     GRCHIDVRNAIMWKDYGMAGTLESARVTTLPIEQASRVSPGSGISAMQMITALEEDVL
                     VPEQKQQVEFTKTGLDLIRSDRGGLVYQPRTGLYCDVAQIDFTSMYPAVIINGNISPE
                     VPLPQGLKPSSPNLGVVPLTLKPLYEKRVKIKQRLLEMPDKEGTVAKSYTARASALKW
                     LLVVCFGFLGYKNARFGRIEAHEAVTRGGREALLLAKEACEDFGFEVLHLFVDALWIR
                     QPGYRAVADFQPVLDEISRRTGMIISLDGIFRWIAFLPSRTNDNQPVPNRYFGVFKDG
                     SLKIRGIEARRRDLPRWVIDTQTDMLNLLAKATDARQLPDYLPDAFKIFTHALDELNS
                     DRVPLESLVLTNRISRDLELYKSPTAAVRAALQLFEQTGKRTDPGQKIRYLYTKNDVY
                     AWDRPGSLSPDQIDKSKYREMMARAAGTVFYPFGIDSKQLSAYAHKSLQFELSLIK"
     gene            complement(4608..5720)
                     /gene="ugpC"
                     /locus_tag="PKV59_01645"
     CDS             complement(4608..5720)
                     /gene="ugpC"
                     /locus_tag="PKV59_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HQP45485.1"
                     /translation="MASVTYDHVWKKFGDVEVLKDIQLAINDKEFLVLVGPSGCGKST
                     ALRCLAGLEEVTSGEILIGDRVVNDISPKDRDIAMVFQSYALYPHMTVFDNMAFGLKL
                     RKTPKEEINRRVNEAAATLGLERLLQRKPRELSGGQRQRVALGRAIVRNPQVFLLDEP
                     LSNLDAKLRVQTRSEISKLHQRLQTTFIYVTHDQVEAMTMADRIAVMYKGIIQQLDTP
                     QNLYNRPDNLFVAGFIGSPAMNFFNAKIKVEEGNVYVVTDSFTVQIPDDRKDTYAPYD
                     GKEIIFGIRPENVHNPEFCPPGIKTAEIEAVVDVIELMGNEIFLYCMTGDTQFVARVD
                     PRSHYSIHDKVKLVFNMEAFHIFDSSEDKENPKAVR"
     gene            complement(5841..6019)
                     /gene="ssrS"
                     /locus_tag="PKV59_01650"
     ncRNA           complement(5841..6019)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKV59_01650"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(6066..>7129)
                     /locus_tag="PKV59_01655"
     CDS             complement(6066..>7129)
                     /locus_tag="PKV59_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45486.1"
                     /translation="IAKYEQEQSRSKRKSAKAPHGVKIKKGVKSFGYCCSVWQRPSVA
                     IYRNLTDSSEMLVSPPTMATNFPSSRLEGIDFGYFNLHGIKGQPNWYGQKDSKDTSSI
                     PMIPVALQIQNLQPVTKAPKVVFAENCYGAEILNRNESNAISLHMIGKGTRVFIGSTV
                     IAYGAMSLPLTAADLLAHLFWKHLMTGISCGEAFRRAKKNLATETESNSGALDGEIQK
                     TLISFIFLGDPLYAVDDNADITDRMQRAKTPRSYELIHERLDSKVAIDTDLAKKIYEE
                     VKEMYKMEGMNDEFSSFTIQKQILSAKSQDDRQRYIESPNYVVVFTKDAKLGNMTDRL
                     ITRVTVSKDGKIKKVSFSR"
BASE COUNT         1945 a         1541 c         1555 g         2088 t
ORIGIN      
        1 gatctaattt cggacagccg atcagagtca catgtttctt aatgaaatcc cgatgaaagt
       61 tattgtaggc aaaagcggta caatcagccg ctatcaataa attacagtta tcaaaaaaag
      121 gtgcatttac gggaaccaat ttcaactgaa ccggccattg cattaattca ctggtttgtg
      181 attcccgact tgattcaatg atcggattgg atgtatctcg tcgcaatgtc tttgcttgct
      241 ttcccggaca accgcttggc acattcgtca tattgttcac tgtcgcttga tgcctcaccg
      301 cttctttatc aaaaggaatc gcttctcttt caatgattgt aatcgcattt gccggacaaa
      361 ccggcaggca atttccgagt ccgtcacaat aatcatcacg aattaatttt gcttttccgt
      421 tgaccatttg aatggcattt tcccggcaag cttcggcgca taacccgcat cctgtgcatt
      481 tttcttcatc gatttccacc atttttcgta tcatatattt gcctcatatg gctgttttat
      541 tttctgatca tattgtaata tactgaatca aaaaaatcgt tgtaaaaaca acaaaaagga
      601 acttatgaaa tcttacgatc cgttactgct gcaaatgccg ctcttttata acttttcccc
      661 caaagatttg gattcgttac gaaactgctt atcgtttcgg gtccggaaat atctgaaagg
      721 ggaagcgatt tttttggaag gtgatgaaat ctcgcgattg ggaattattc tgaacgggag
      781 cgttattatt gtcaaggagg atatttcggg aaatcgcaat attatttcaa ccttttcaac
      841 cggaaatctg ttcggagaag cttttgcatt ttcaaaacaa aaacggatta tggtttctgt
      901 tttttccgaa tcggactccg aaattctatt tttggatgcc gaacatttac tgacactttg
      961 caaagaacta tgcccgttcc attctcaact gatccgtaat ttgatgaatg aattcgcgga
     1021 aaaaacaatt ctgttaaacg aaaaaatcga tttattatct caaaaaacga ttcaggacaa
     1081 agtgttggcg tttcttttca gcgaagcaaa acggcagagc agccgttccg tgcaaattcc
     1141 ttttaatcgt cagcaacttg ccgattatct gtttgttgat cgcagtgctc tttccgctga
     1201 attaagtcgt atgcaaaaaa aaggaatttt ggaatatcat aaaaataaat tcgtcctaaa
     1261 aacgatagct catttcgatg acaattcttc tttcaatttg taaaaacaag attgattgat
     1321 tccttgacat atagaatatt tgttctataa tcagaaatat ggaacaaaaa ctttcttttt
     1381 gcacatctga aattctgatt cggaatatgc gttccccgct tccttatggg ttgacggtta
     1441 ttcaagggcc tcatgccgcc agcgggcttt tactgcaaat ttgcgcaaag ctcgcattga
     1501 gaggtcctgt gcggatttta gactgcggga atcgcagcga tatgtatcag gttgccagag
     1561 aattacgtcc gttgacacgt gatccggcaa ccgctatgca gcgaatccac ttgtctcgag
     1621 cttttacctg ctatcaagcg gaagctctgc tgggtcagaa ccgcggacaa tctcaaacgc
     1681 cgattctgat ctttgattta ttggcaacat acttagacga aagcgtttcc aaacaggaag
     1741 ccgaacggtt atttgatgac acgttggatc atctgcaaaa aatgagccga aaaaacccgg
     1801 tattggttgg gattcgaccg ttgccggcga ttgctcttga ccgcagcaat ttaccgttgc
     1861 gactgcaaaa agccgcggat gaatcttttc agcttatgga gatatcacag aaagcggaaa
     1921 caaatgatga tcaactgtcg ttgttttgaa acagcggaag gagcgatttt atgggaagga
     1981 caattcgatc tgctacgcaa acttggatag aggaagagaa agcgttagga cgatttgcac
     2041 gtgctttgcg gaaagaagat caacgtgcaa tgatggagct cattaatctt tcgcggcttc
     2101 atatcgcgga atcttcctat gccagcaacc tctatccgat ggatgtttat ttaatctcca
     2161 tgcttttgga gatgtttaaa agagtacagc gtgatgaagc aacgttggct gaggtctgta
     2221 aacatgccgg catcgaattg ccgtctgttt cgactgaaat tcccgaattc ccggatttgg
     2281 tcgacctgat tgaatcgctg gatcaggctg atgcgtaact gttttgtcta tgcaaatgca
     2341 tgccaggggt tggctgcttg acctttatga agatgcgaac gacggcgttc gactttggtt
     2401 cattttggaa aatgatcaac ggatttgttt aaaacaacaa ttgccgatta cattttatat
     2461 ttccggagat tcaaaacgtt tacatcagtg ctgcctcttt ctgaaacatc tgccgggcat
     2521 gattgcaatg caacgtgtca gcaaaaaaga tgtctttaaa accgagccgg tatgtgcatt
     2581 acaagtcgat atggccgacc ctgttcatca gcgtcaagca tttttaaagc ttaaaaaaga
     2641 ttttcccgat ttgacttact ataatgccga tcttgccgta catattcatc atgccgctct
     2701 ttaccaaact ttcccgatgg cgttttgtga tttgttatac gattcggaaa gcggtttaat
     2761 tcaaatgtta aaagtgctta acagccgttg ggatattacg cctctgcacc ccgtgttacg
     2821 aatccttgaa attgcgcccg actgtgatcc gatccgaaag aaaccggaaa tgctaaaaat
     2881 atgcgaccgc cgcggccata aacaaacaat ttgtttacgg gaaccgacac ttcttttgga
     2941 gcatctgaac gcattgatag cagcaatcga tccggatatt gtggaaaccc aatggggcga
     3001 tgactggttg ttcccgttgt tattgcagtg ggaacaggaa acgggaattc cgctgaattt
     3061 taatcgagac ccggaacgaa agcttcgttg gaaaaaagaa ataacttatt tttcatacgg
     3121 ccaaattgta tatcggggtc gggaagcgca tttattcggt cgctgccata tcgatgttcg
     3181 caatgcgatt atgtggaaag attacgggat ggccgggaca ttggaatcgg cacgggtaac
     3241 gacattgccg attgagcagg cttcacgtgt atcgcccgga tcgggaattt ccgccatgca
     3301 aatgatcacg gcactggaag aagacgtact ggttcccgag caaaagcagc aggtggaatt
     3361 cacaaaaaca ggactggatc tgatccgctc cgaccggggc ggtctggttt atcaaccgcg
     3421 caccggttta tattgcgacg tggcacaaat tgatttcacg tcgatgtatc ccgctgtgat
     3481 tatcaacggc aatatctctc cggaagtgcc acttcctcag gggctgaaac cttccagtcc
     3541 gaatctgggt gttgttccgt tgactttaaa accgctttat gaaaagcggg taaaaataaa
     3601 acaacggctg ttggaaatgc cggataagga aggaactgtc gcaaagagtt acaccgccag
     3661 agcctctgct ttgaagtggc tgctggtagt ttgcttcgga tttttaggtt ataagaatgc
     3721 gcgattcgga cgaatcgaag cacatgaggc ggtcactcgc ggcggccgcg aggcgctgtt
     3781 gttagcaaaa gaggcttgcg aagatttcgg tttcgaagtg ctgcatttgt ttgtcgatgc
     3841 attatggatt cgtcagccgg gttatcgggc ggttgcggat tttcagccgg ttctggatga
     3901 aatttctcgc agaaccggta tgatcatatc gttggacggc atttttcgtt ggatcgcatt
     3961 tttaccgtcc cgcacgaatg ataatcaacc ggtgccgaat cgctatttcg gtgtatttaa
     4021 agatggaagc ctgaaaattc gggggattga agcccggcgg cgggatttac cgcgctgggt
     4081 aattgacacc caaacggata tgttaaattt actggcaaaa gcgactgatg ctcgccaact
     4141 cccggattat ttgccggacg catttaaaat cttcacccat gctttggatg agctcaactc
     4201 ggatcgggta ccgttggaat cattggtcct gaccaatcgt atcagccggg atttggaatt
     4261 atataagtca ccgacggcag cggtacgagc tgcgcttcaa ttgttcgaac agaccggaaa
     4321 gcgcacggat ccggggcaaa agattcgtta tctttataca aaaaatgatg tttatgcttg
     4381 ggatcgcccc ggaagtcttt ccccggatca gattgataaa tcaaaatatc gagaaatgat
     4441 ggcgcgggcc gccggtacgg ttttttatcc gttcgggatt gattcgaagc aactttcggc
     4501 atatgcccat aaatcgcttc agtttgaatt aagcttaata aaataaaaaa gaccaaacgt
     4561 ctttttttga taggagtttg gtctttctga accgaaaaag atcgagatta acggaccgcc
     4621 ttcggatttt ccttatcttc ggacgaatca aaaatatgaa acgcttccat attgaaaacc
     4681 agtttgactt tgtcatgaat ggaatagtga ctgcggggat ctacccgggc aacaaactgt
     4741 gtatcgcccg tcatacaata caagaaaatt tcattcccca ttaactcgat tacatcgact
     4801 accgcttcaa tctcagcggt tttgattccg ggcggacaaa attcgggatt atggacattt
     4861 tcaggacgaa taccgaagat aatttctttt ccgtcatacg gtgcataagt atcttttcga
     4921 tcatccggaa tttgaacagt gaacgaatcc gtcacaacgt atacattccc ttcttcaact
     4981 ttgatttttg cattgaaaaa gttcatggcg ggagaaccga taaacccggc aacaaagaga
     5041 ttatccggtc tgttgtacaa attctgcggt gtatccaatt gctgaataat tcccttatac
     5101 attacggcaa ttcgatccgc cattgtcatt gcttccactt gatcatgggt tacgtaaatg
     5161 aaagtggttt gaagccgttg gtgcaatttg ctgatttcag aacgagtctg aactctcaat
     5221 ttcgcatcca agtttgacaa cggttcatcc aggaggaata cttgcgggtt acggacgatg
     5281 gcacgtccaa gcgcaactct ttggcgttga ccgccggata attcacgcgg tttccgttgt
     5341 aaaagccttt ccaagcccaa agtcgccgca gcttcattaa cacgccggtt tatttcttct
     5401 ttcggtgttt tgcgaagttt taaaccaaaa gccatattat caaaaaccgt catgtgggga
     5461 tacaatgcgt aggattgaaa taccattgcg atatcccgat ctttcggaga aatgtcgttt
     5521 accacacgat ccccgattaa tatttcgccg gatgtaactt cttcaagtcc cgcgagacat
     5581 cgtaatgcgg ttgatttgcc gcaacccgac ggacccacca aaaccaaaaa ctctttatcg
     5641 tttattgcga gctgaatgtc tttaagtact tcaacatcgc caaacttttt ccaaacatgg
     5701 tcataagtta cgcttgccat ttaataattc ctccattccg tatgggtata gtggttttat
     5761 tatacgatga aacttattaa aattacagag agaaaatttc gacgatccgc aatatttatc
     5821 tttcaacgaa aaacgataaa aaagaccccg ccatgccgtg tgctgctgag ttttgaacct
     5881 gctatcgcag gacggtttcc cgttttccaa acgcagcctt ggctttcgcc tatcgcattg
     5941 tcgggaatac cggaaaaccc atttttactc gtgttggccc aaaaccgttt ttacagcatc
     6001 acctgcatag cagggtatgt ctgttttata ccataaaaac gaatcattga acaggcaaaa
     6061 agcccttatc ttgaaaaaga aaccttcttg attttgccgt cttttgaaac ggttacccgc
     6121 gtaattaatc gatcggtcat gtttcccaat ttggcatctt tggtaaaaac aacaacatag
     6181 ttgggacttt caatatatcg ctgtcgatcg tcttgtgatt ttgcacttag gatttgcttt
     6241 tgaatggtaa aggaagaaaa ttcatcattc atcccttcca ttttgtacat ttcctttact
     6301 tcttcataga tctttttagc caagtcggtg tcaatagcca ctttactgtc caatcgttca
     6361 tggatcagct cataacttct cggagtttta gctctttgca ttctgtcggt tatatcagca
     6421 ttgtcatcca cagcatataa cgggtcaccc aagaaaataa aagaaattaa tgttttctga
     6481 atttctccgt ctaatgctcc cgagttggat tcggtttcgg tcgctaagtt tttcttggca
     6541 cgcctgaatg cttctccgca tgaaatacct gtcataagat gtttccaaaa cagatgagca
     6601 agcaagtccg cagcggttaa aggtaaactc atcgcaccgt aggcaataac agttgatccg
     6661 ataaagacac gcgttccttt tccgatcata tgtaaagaga ttgcatttga ttcgtttctg
     6721 tttaaaattt ccgcaccgta acaattttcg gcaaaaacta ctttcggtgc cttcgtaaca
     6781 ggttgaagat tctgaatttg caaagcgacc ggtatcatgg gaatggaaga agtatccttt
     6841 gaatcttttt gtccgtacca attcggctga ccctttattc catgaagatt aaaatatccg
     6901 aaatcgatcc cttccagtcg ggaggaagga aaattggttg ccatggtcgg aggagaaacc
     6961 aacatctccg aactgtccgt cagattacgg tagatcgcaa cggacggacg ttgccaaacg
     7021 gaacagcaat agccgaagct tttcaccccc ttcttgattt tgacaccgtg aggagctttt
     7081 gcggattttc tctttgatcg ggattgttcc tgttcatatt ttgctatgt
//
LOCUS       DAOMIV010000037         7120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_10630_length_7120_cov_4.325124, whole genome shotgun sequence.
ACCESSION   DAOMIV010000037 DAOMIV010000000
VERSION     DAOMIV010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10630_length_7120_cov_4.325124"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKV59_01660"
     CDS             complement(<1..528)
                     /locus_tag="PKV59_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HQP45487.1"
                     /translation="MANEPKKNEKKKAENEPQDDWENIAEDIDITEDMDMANWDRDLD
                     LNLDWSDEDLLLDSANYEDPVRLYLKEIGMVDLLSTDDEFRLATCIAAIDFMDRCEAA
                     IGDVSEDRFIESLYREICVQLSKAWEKSTEAIKAYKEEYPLPDLVQMTTEAQQLLRAW
                     KSESPSYIRSYLDNGR"
     gene            complement(518..2479)
                     /gene="dnaG"
                     /locus_tag="PKV59_01665"
     CDS             complement(518..2479)
                     /gene="dnaG"
                     /locus_tag="PKV59_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQP45488.1"
                     /translation="MSQIDEIKNQIDIVDLVSETVKLRRTGKNYIGFCPFHPNTRTPS
                     FVVFPDSGSWRCFGQCAEGGDIFSFLMKRDNLDFQQALQVLANRAGIKLETFKQEDKT
                     KKEQINNLHQLMQNAIDYYHNNLVHNPIGKPALDYLHKRGLTDETIHNFEMGYAPDSW
                     DALLQHLLSMNAGRQDLIDVGLVSEQRDDKGEIIKDGKIFDRFRNRIMIPIHDSSGNP
                     IGFGARILDPNDVPKFLNSPQTVLFDKGKTLFGLNRAKSSIREKNQAVIVEGYLDVII
                     LHQAGFTNTVSPMGTALSTDQAKLLARQSKNMILALDADAAGDVATIRGIDVIRSAVK
                     NDKEETVESQTLIRQERKLNADIRITRIPEGMDPDEVVLRDPSEWQQILDNAKPVVIF
                     MMETLANGRNLEDAKTKSEIAKQIMPLIREVVDPIERETYRQQLARFLKIDENLLNYI
                     SPQKSKKSNQWEIPDRLDFQSKPSLVFDPKEGIYSKEMTILQFLYHFYNTPGAISKID
                     RNLRKYNLGPLRKEDFEESDLRVIAGCYFDGLNQDDELNTQKYIIENIPDSIRPTFEK
                     IRKPNFKGSINPVDLDIDLTRSVAYLRNEKNIFNQLELQTLYKEQLESGSNPEEYDRL
                     IDQLIHERKNLEKLLKSLDQRLTEEPNGK"
     gene            complement(2681..3460)
                     /locus_tag="PKV59_01670"
     CDS             complement(2681..3460)
                     /locus_tag="PKV59_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQP45489.1"
                     /translation="MDFKTFGNQKNPSLLLLHGLAMTWKSFDEIVPLLMDDYFLVIPV
                     LDGHNPDNRSNFQTVQEEAEKIEKKVVDDFDRHLSGVLGFSLGGTIAIELLTRNKIQM
                     DRVILDGACASTPGSLSRPYASMWISMLSKFTDGKNISSFLKKVTGSGDVEALYNMLF
                     FNLSEKTITNACKAAFEYEISPYVGQTKAQVTFWHGQREPYPAKTAKKMKEYLPAMRI
                     RIFGNLGHGELFSRFPQRYVAEIRNFLLPENQSTDALQTEQ"
     gene            complement(3609..4094)
                     /locus_tag="PKV59_01675"
     CDS             complement(3609..4094)
                     /locus_tag="PKV59_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQP45490.1"
                     /translation="MERYQVIPRTLIFIFHDEKILLLKGAPDKKNFPHLYNGLGGHVE
                     RGETILECARRELKEECGLTLLNLTLCGILTDITGEQVGIQIHLFKGSVDQLADHLHS
                     SSEGTIGWFRKDEVAHLELVPDVPFYLETVWNWKPGDPLIFGIIDRRNTPDNLIRFTA
                     A"
     gene            complement(4097..4297)
                     /locus_tag="PKV59_01680"
     CDS             complement(4097..4297)
                     /locus_tag="PKV59_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45491.1"
                     /translation="MKDEKQGCLSGLLQLFLVKNIYDWAQRKFGYGNGILGCGCGCLL
                     MVVFIIVVFSILTKTNFLDFSF"
     gene            complement(4394..5833)
                     /locus_tag="PKV59_01685"
     CDS             complement(4394..5833)
                     /locus_tag="PKV59_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45492.1"
                     /translation="MKKTIRSFALAILLSLFVGIFTILAQTDGGVIQFGAGESSAWIS
                     GSLAPNESIRYSFYALSGQIVSISVKSANNSAIIGFSNSKDYFSYLSPDRKYMYYTMP
                     LPQTGTYYLTVQDTGPGTDFVLQLTIPPLNQVPVQPVQSGQPVYSQPKSLSGGTIQFA
                     EGQSSATISGWLDAGSDITYQFYASKNQVLSLAVTSTNGSVFAGIQDGTGYIYLSPEK
                     RWQYYISPLYSTGPYYLTLFNPGGGTNFSFQITIPPLNQTGPSVPILPTTQPVPVMPY
                     PISQNGGAIQFAPGETSAWISGNVSANNCIRYSFFGGNNYKMIATLNSATGSVSLGVS
                     DIYGTVYLNSFNRSNYWMMPLKQTATYFLDVCNYGQQPADFTLNLVIPVNIPTSYAVK
                     TGTLRSNSVVSYTIFGHADQVMTVNLNSGATPQAYLRIMGLGDGVVYLDNNAFQTYWS
                     GTLPGDQDYLIDVVAFGAASNYQLSVSLE"
     gene            complement(5893..>7120)
                     /locus_tag="PKV59_01690"
     CDS             complement(5893..>7120)
                     /locus_tag="PKV59_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45493.1"
                     /translation="PEKAAPENPTLLPGIQPSPSVTQTKTAENHSSPQYSSTSTPIAI
                     PDLTVTLQPTAVPLRLIATAAPLKVQRDHSKAFLITLSIPSVTLSPTYTRTLEMTRTS
                     IPTSSPIPATATKLFSTATKVTEPTEISTATRTASATLQKTIALFLSPTAAATETAIP
                     TAMKGFVKPTETPIPVIFITPIIVCENNGTCVKNTVTPTQISNFELIHFSEFDNSVVV
                     SGEIKSESEKTYTFIANKDQVLRIGLASSSLSKPDLILQNSIGTNIEPQQSANEMFFN
                     LNENGWYNLKITAPDHDLSYNLELSLPERLSFSKNQSVIHLSDRINASSTRRFTIEIP
                     AGKYLKLSLKILNGEPVDFHIYDLSSGKENSLNVNRAMAWQSGKDGHKFLIEMKSVSV
                     SVDFELTLESVQPEKL"
BASE COUNT         1914 a         1528 c         1569 g         2109 t
ORIGIN      
        1 tcttccatta tccagataag agcgaatata agaaggactt tcacttttcc aagctcgcag
       61 taattgctgc gcttccgttg tcatttgaac taaatccggc agcggatact cttctttata
      121 agccttaatg gcttccgttg atttctccca tgctttactg agttggacac aaatctcccg
      181 ataaagactt tcgatgaacc ggtcttcgga aacatcgccg attgccgctt cgcaacggtc
      241 cataaaatcg atcgcggcga tacaggtcgc gagtcggaat tcatcatcgg tacttaataa
      301 atcaaccatc ccgatttctt taagatacag acgaacagga tcctcataat tggcgctgtc
      361 taaaagtaaa tcttcgtccg accaatcaag attcagatcc aaatcccggt cccaattcgc
      421 catatccata tcctctgtga tatcgatatc ttctgcgata ttttcccaat catcctgcgg
      481 ctcattttca gcctttttct tttcgttttt tttcggttca tttgccattg ggttcctcgg
      541 ttaagcgttg atcaagactt ttcagcagtt tttccagatt tttcctctca tggatcagtt
      601 gatcgattaa acggtcgtac tcttccggat ttgatcccga ttcaagctgc tccttataca
      661 gagtctggag ttccaactga ttaaatatat ttttctcgtt tcgcagatag gcgacggaac
      721 gggtcaaatc aatgtccaaa tcgaccggat tgatgctccc cttgaaattc ggtttgcgga
      781 ttttttcaaa tgtcggcctg atcgagtccg ggatattctc gataatatat ttttgtgtat
      841 tgagctcatc atcctgatta agcccgtcga aataacagcc ggcgatcaca cgcaaatccg
      901 attcctcaaa gtcttctttg cgtaaagggc cgagattata ttttctgagg ttccgatcga
      961 tttttgaaat agcgccggga gtattataga aatgatataa aaattgcagg atggtcattt
     1021 ctttactgta aattccttct ttcggatcaa aaacaagaga aggcttcgat tgaaaatcga
     1081 gacgatccgg aatttcccat tgatttgatt tctttgactt ttgcggagaa atataattca
     1141 gcagattttc atcgatcttc agaaagcgtg ccaactgttg tcgataggtt tcccgttcaa
     1201 tcggatcgac aacttcgcgg attaatggca tgatttgctt ggcaatttca ctttttgtct
     1261 ttgcatcttc caaatttcga ccgtttgcca atgtttccat cataaaaatc acgacgggct
     1321 tggcattatc caaaatttgt tgccattcgg acggatcccg taaaactacc tcatccgggt
     1381 ccattccttc ggggattcga gtaattcgga tatcggcgtt cagcttccgt tcttgacgga
     1441 tcagcgtttg gctttcgacc gtctcttctt tatcgttttt tacggcggaa cgaatcacat
     1501 cgattccgcg gatggtggcg acatcaccgg ctgcgtcggc atccagtgcc aagatcatgt
     1561 ttttggattg gcgcgccaat aattttgcct ggtccgtact taatgcggtt cccatcgggg
     1621 atacggtatt ggtaaaaccg gcttgatgca gaatgattac atccaaatac ccttctacga
     1681 tgaccgcttg gtttttctcg cgaatggaag attttgcccg atttaaacca aagagcgtct
     1741 ttcctttgtc aaaaaggacg gtctgaggag aatttaaaaa tttgggtaca tcgttcggat
     1801 cgagaattct ggctccgaat ccgatcgggt taccggatga atcatggatc ggaatcatga
     1861 tccggtttcg aaagcggtca aaaattttac cgtctttaat aatctcgcct ttgtcgtctc
     1921 gttgctcact gacaagcccg acgtcgatca gatcctgccg gccggcgttc atcgataaaa
     1981 gatgttgaag caaagcgtcc caagaatcgg gggcatatcc catttcgaag ttatgaatgg
     2041 tctcatccgt gagacctctc ttatgaagat aatccaatgc cggtttccct atgggattat
     2101 gaaccaaatt attgtgataa taatcaatgg cattttgcat cagctgatgc aaattattga
     2161 tttgttcttt ttttgttttg tcttcctgtt tgaaagtttc caacttgatt ccggcccgat
     2221 ttgccaaaac ctgaagggct tgctggaaat caagattgtc gcgtttcatc aagaagctga
     2281 aaatatctcc gccttccgca cattggccga agcagcgcca gctgcccgaa tccggaaata
     2341 caacgaaaga aggcgtacgc gtattgggat gaaacggaca aaagccgata taattctttc
     2401 ccgttcgtcg tagttttacc gtttccgaga caagatcaac gatatcgatc tgatttttta
     2461 tttcatcaat ttgtgacaat gaaatccctc tcgttcaatt ttacaacaat aatcggacgg
     2521 cattgttaag ggacaaagta atcgattggg attatttgat aaatttgaaa aaagaactta
     2581 ttgggcggag gaagaccggc cggagatttt tcaagaactt ataaaaattc aacggttcga
     2641 tatcggagca ttttctctat tccgataagg atggaaaatt tcattgttcg gtttgaaggg
     2701 catccgttga ttgattttcc ggtaggagga aattccgaat ctccgcaaca tatcgctgcg
     2761 gaaagcggct gaataattct ccgtgtccta aattcccgaa tattctgatt cgcatggccg
     2821 gcaaatattc tttcattttc tttgctgttt ttgcggggta cggttctcgt tggccgtgcc
     2881 aaaaagttac ctgagctttg gtttgtccga cataaggcga gatttcgtat tcaaaggcgg
     2941 ctttacaggc atttgtgatt gttttctcgg acaaattaaa aaataacata ttataaaggg
     3001 cttcaacatc tccgcttccg gtcacttttt tcaaaaaaga agagatattt ttaccgtccg
     3061 taaatttgga taacatagag atccacatgg acgcatacgg tctgctgaga cttcccggtg
     3121 tgcttgcgca agctccgtcc aagattaccc gatccatttg gattttattc cgggtcaata
     3181 attcaatcgc aatcgttcct cccaatgaaa atcccaagac tccgctgaga tggcgatcga
     3241 aatcatcgac aacctttttt tcaatttttt ccgcttcctc ttggacggtt tgaaaattgg
     3301 atcggttatc cggattatgt ccgtccagca ccggaattac caaaaaataa tcatccatca
     3361 ataacggaac tatttcgtcg aaagatttcc aggtcatcgc taagccatgt agcagcagta
     3421 aagaaggatt tttctgattt ccgaaagttt taaaatccat gaatatggtg cctttctttc
     3481 agagaatgaa acgaatattt acccattata atcaagccgg cggcttatca acccaaaatg
     3541 ataattttgt ttatcgattg ggaaagaacc gataaaaaca attcgcaagc agaaacatat
     3601 aataaaaatt acgctgccgt aaatcggata aggttatccg gtgtatttct ccgatcgatg
     3661 atcccgaaaa tcaaaggatc cccgggcttc caattccaaa ccgtttccaa ataaaaaggg
     3721 acatcgggta ccagctctaa atgagccact tcgtccttgc gaaaccaacc gattgttcct
     3781 tcggaagagg aatgaagatg atcggctaat tgatcgacgg accccttaaa taaatggatt
     3841 tggattccca cttgctcgcc ggtaatgtcg gtcaaaatcc cgcacagtgt cagatttaag
     3901 agtgtcaatc cgcattcttc tttcaattca cgacgtgcac attcaagtat cgtttcgcct
     3961 cgttccacgt gtccacccaa tccgttataa agatgcggga aattcttttt atccggtgct
     4021 ccttttaaca gaagaatttt ttcatcatga aatatgaaga ttaaagttct gggaatgacc
     4081 tgatatcttt ccattttcag aaactgaaat ccaagaaatt tgtcttggta agaatggaaa
     4141 agacgacgat gataaaaaca accattaata gacaaccgca gccgcagccc aagattccgt
     4201 tcccgtaacc gaattttctt tgtgcccaat cataaatatt tttaactaaa aaaagttgca
     4261 gcaaaccgct caaacaaccc tgtttttcgt ccttcattta tctgcctttc taatatgtgt
     4321 ttgtgtttta aatcagaaag ggcatccttt ttctttacag ggacaccctt tccggtggaa
     4381 ttgctttgtc ccgttattcg aggctgacgg acagctgata attcgatgcc gctccaaatg
     4441 ctaccacatc gatcagataa tcctggtccc ccggcaaggt tccggaccaa taggtttgaa
     4501 aggcattgtt atccaaatat acaacaccgt cacccaatcc cataatacgt aaataggctt
     4561 gcggagttgc accgctgttt aaattaacgg tcattacctg atctgcatga ccgaaaatgg
     4621 tgtaactgac gacgctgttg cttcgaagtg ttccggtttt tacggcataa ctggtgggaa
     4681 tatttaccgg aatgaccaga tttaatgtga agtcagcggg ttgctgccca taattacaga
     4741 catccaaaaa gtaagtggcg gtttgtttca acggcatcat ccaataattc gatcgattga
     4801 aactgttgag ataaactgtt ccgtagatat ccgaaacacc gagactgacc gatccggttg
     4861 cggagtttaa cgttgcgatc attttgtagt tattccctcc gaaaaaggaa tagcggatgc
     4921 aattgttggc tgaaacgttt cctgaaatcc atgcggaagt ttcacccggg gcaaattgaa
     4981 tcgctccgcc attttgggat atcggatatg gcattaccgg aaccggttgg gttgtcggaa
     5041 gaatcggaac ggatggaccg gtctgattga gaggcgggat agtaatttgg aagctgaaat
     5101 tggttccgcc gccgggatta aataaagtca gataataggg gccggtactg tacagaggcg
     5161 aaatataata ttgccatctt ttttcggggc tcaggtagat atatcccgtt ccgtcttgga
     5221 taccggcaaa gacactcccg ttggtcgaag tgaccgcaag ggacagaact tgattttttg
     5281 atgcataaaa ctgataggtg atatcacttc ccgcatccaa ccatccgctg atcgttgcgg
     5341 aggattggcc ttcggcgaat tgaatcgtgc ctccgctcag ggatttcggt tgagaataaa
     5401 ccggctgacc ggattgaacc ggctgaacgg ggacttgatt cagtggcgga atagtcagct
     5461 gcaaaacgaa atctgttccg ggaccggtat cttgcacggt aagataataa gtaccggtct
     5521 gcgggagcgg catcgtgtag tacatgtatt tacgatccgg actcaaataa gagaaatagt
     5581 ctttggaatt actgaacccg atgatggcac tattgtttgc ggatttaacc gaaatcgaaa
     5641 cgatctgacc ggaaagcgca taaaaagaat accgaatact ttcattcggt gccagacttc
     5701 ctgagatcca ggctgaactt tctccggcgc caaattgaat gaccccgccg tcggtctgtg
     5761 ccaaaatcgt aaaaatgccg acaaacagcg ataaaagtat cgcaagagca aaggaacgga
     5821 tcgttttctt catttaatcc tcccgaaata ttctgaatcg gaatatttca tctttcattt
     5881 attttactca atctacaatt tttcaggctg aacgctttca agcgtcagtt caaaatcaac
     5941 cgagacagaa accgatttca tttcaatcag gaatttatgc ccgtcttttc ccgattgcca
     6001 tgccatcgct cggttgacat tcaatgaatt ttctttaccg gaacttaaat cgtaaatatg
     6061 aaaatcaacc ggctctccgt tcaagatttt caaagacagt tttaaatatt ttcccgccgg
     6121 aatctctatt gtgaagcggc gggtggagga tgcgttgatc cgatccgata gatgaatgac
     6181 cgattgattt tttgaaaaag agagtctttc aggtaatgaa agctctaagt tgtagctcaa
     6241 atcgtgatcg ggagctgtga tttttaggtt ataccaaccg ttttcattca agttgaagaa
     6301 catttcattt gccgattgct gcggttcaat gtttgttccg atactgtttt gcaaaattaa
     6361 atcgggtttt gacagcgacg aagaagccaa tccgattcgt aatacttgat ctttgtttgc
     6421 gataaacgta taagtttttt cgctttccga ttttatttct cccgaaacaa cgacggaatt
     6481 gtcaaactcc gaaaaatgaa tcaattcgaa attggagatt tgggtcggcg taacagtgtt
     6541 cttcacacac gttccgttat tctcacagac aatgatcgga gtgatgaaaa tcaccggaat
     6601 cggagtttcg gtcggtttta cgaaaccctt cattgcggtc ggaatagcgg tttcggttgc
     6661 cgcagcggtc ggtgaaagaa aaagcgctat tgttttttgg agagtcgcgg atgcggtccg
     6721 ggttgcggtg gatatttcgg ttggttcggt cacttttgtt gccgtcgaaa aaagttttgt
     6781 cgctgttgcc ggaatggggg aagatgtcgg aattgaagtt cgcgtcattt ccaaagtcct
     6841 cgtataagtc ggtgaaagtg ttacggacgg aatagaaagg gtaatcagaa aagctttcga
     6901 atgatctctt tgcactttga gtggagccgc ggtagctatc agtctgaggg ggaccgctgt
     6961 aggttgtaaa gtgacagtca aatcgggaat ggcgataggc gtcgaggtgg atgaatattg
     7021 cggagaagag tgattttccg ccgttttggt ttgagtgact gaaggagacg gctgaatacc
     7081 cggcaagaga gttgggtttt cgggtgcggc tttttccggt
//
LOCUS       DAOMIV010000038         7030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_10805_length_7030_cov_4.287312, whole genome shotgun sequence.
ACCESSION   DAOMIV010000038 DAOMIV010000000
VERSION     DAOMIV010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10805_length_7030_cov_4.287312"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PKV59_01695"
     CDS             <1..248
                     /locus_tag="PKV59_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45494.1"
                     /translation="LLSCCYDDALPQGLGVYLPDNEKWENDQWVTVKGHWDRIRFGEN
                     SRYLIIPESISEAEPPEDPYIYDKTEPAETNSANENI"
     gene            complement(320..808)
                     /locus_tag="PKV59_01700"
     CDS             complement(320..808)
                     /locus_tag="PKV59_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQP45495.1"
                     /translation="MLSKQMQQALVEQINKEFHSAYIYLGMEAYFENENYKGMSAWMN
                     KQVKEEISHAMKFFRYVYERSGEVSLDTIEKVSVDYKNPLDVFKAGLEHEKFITSSIH
                     GLYDLALEEKDYGTQELLRWYISEQVEEEANFEYIIAMLEKSGDNPGGLMILDEKLGA
                     RE"
     gene            complement(982..2832)
                     /locus_tag="PKV59_01705"
     CDS             complement(982..2832)
                     /locus_tag="PKV59_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQP45496.1"
                     /translation="MRPIPYGLWKSPISEQAVAQRGRINTLKWGSDNRLYFSGNRGGN
                     SFLYEYRCDSGLKKLNGDLPVGGNIGYGGGDFDVRNEKVLFSAGKRGLFLQNLNKPAF
                     KLLTNDRYMTASPVISPNGRFVAYISGDGTDDQIALLRLDERSWPFLWVHGADFYMQP
                     AWSPRGDYFTWVEWNHPDMPWDGSKVMIGKVDPENGTISEIVHIAGKMPFPASQPKFS
                     PDGKMLSYIRSNGEWEDLILRNLESGEEKILVHGDHFALTVPAFAQGVQTYDWFPDSR
                     QIAFLQIFGTRSKICIVSVESGEIRILNSPQYSSFDTISVSPETGMIAAVADSPLQPT
                     QIILINENEIKTVFRISDDLINEEYISKPREISWEAPDGTPVYGIFYPPTNPDAHWVG
                     LPPAFVHIHGGPTGKADLRFNPEIQFFTSRGFGYLEVNYRGSCGYGTGYLKSLYGNWG
                     VYDVEDAVLGASCLSALHLADRNRLLIIGGSAGGFTVLNTLIQYPDSFKAAACLYGVT
                     DLFGLAINTHKLELHYTDSLVGPLPEAAPLYEERSPIRHVEKIKTPLAIFQGDADPVV
                     IPSQSNELVARLKCPHVYRLYKEEGHGFRRPETITDYLSTLYSFATQYLL"
     gene            complement(3168..3554)
                     /gene="rplL"
                     /locus_tag="PKV59_01710"
     CDS             complement(3168..3554)
                     /gene="rplL"
                     /locus_tag="PKV59_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQP45497.1"
                     /translation="MADIEKLVEALSSLSVLEASELVKALEDKWGVSAAAPVATVAAA
                     PVAEAAAPAEEATEFDVIIKDAGPKKIETIKVIRVLTNLGLVEAKQMAETAGAKVLSA
                     VGKEAANDAKSKLEAAGATVEVKPAS"
     gene            complement(3604..4131)
                     /gene="rplJ"
                     /locus_tag="PKV59_01715"
     CDS             complement(3604..4131)
                     /gene="rplJ"
                     /locus_tag="PKV59_01715"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQP45498.1"
                     /translation="MAFTKSEKQKMMEQYSQWLQKSQGVFMLEYSKMDMPAIDEIRAK
                     AREAGGEVHVVKDTLIQKVLEKENYKLQKDITGTSLVGFAFTDAAAMAKVFSDLVKNE
                     AFKIKGGFLDKNEISESQVKALASLPPLPVMRAKLLGTILAPASQLTRVIAEPGRSLA
                     AVIQAHISKEEPTAA"
     misc_feature    complement(4155..4290)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(4329..5048)
                     /gene="rplA"
                     /locus_tag="PKV59_01720"
     CDS             complement(4329..5048)
                     /gene="rplA"
                     /locus_tag="PKV59_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQP45499.1"
                     /translation="MAKHGKKFQEAQKKVNPSTNYAPKDAIDLVKETSITKFDATVEI
                     HLRTGLDPRQADQQVRDVVVLPNGLGKTVRVLVFAQGEGAAKAEEGGADIIADSDEWI
                     QKIQQGFTDFDVAIATPDMMAKAGRLGRVLGPRGLMPNPKAGTVVPAEDLPRVIKEAK
                     AGRVEFRLDKSGNIHAPIGKVSFDQKALYENLAAFLDAVRKARPAGAKGTYIKKITLA
                     STMGPGIHVDLSAAQGLEPIA"
     gene            complement(5100..5525)
                     /gene="rplK"
                     /locus_tag="PKV59_01725"
     CDS             complement(5100..5525)
                     /gene="rplK"
                     /locus_tag="PKV59_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQP45500.1"
                     /translation="MAKKLKAIVKLNLNAGKANPAPPVGPALAGHGINIMAFVKEYNA
                     RTQTRIGEIVPAEISVYTDGSFTFVLKTPPASYMIKRAAGIETASPEGSKGRVGKITR
                     AQVREIAETKLKDMNAVDIEGAMKQIEGTARNMGIQVVD"
     gene            complement(5627..6163)
                     /gene="nusG"
                     /locus_tag="PKV59_01730"
     CDS             complement(5627..6163)
                     /gene="nusG"
                     /locus_tag="PKV59_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQP45501.1"
                     /translation="MESNEERNWYVIHCYSGYENKVRHNLEQRIDSMGMKDKIFDVVI
                     PTQEEIEVKEGKRKTIERHIFPGYVLVNMILTEESWFIVRNTPGVTGFVGMGNQPTPL
                     RPEEVNSIIKRMEAEAPVVSVTYKVGEKVRIIDGPFNDFRGTVAEIDQDKSKVRVMVN
                     FFGRETPVELDFLQVEKA"
     gene            complement(6333..6575)
                     /gene="secE"
                     /locus_tag="PKV59_01735"
     CDS             complement(6333..6575)
                     /gene="secE"
                     /locus_tag="PKV59_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQP45502.1"
                     /translation="MADTKIKKTEKPAKPSAPKKPNKIAKWWNETIGELRKVTWPTRE
                     EALHMTKIVLIVVFAMAVFLGIVDFLFSELIGTIVG"
     gene            complement(6647..6720)
                     /locus_tag="PKV59_01740"
     tRNA            complement(6647..6720)
                     /locus_tag="PKV59_01740"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6684..6686),aa:Trp,seq:cca)
     gene            complement(6767..6937)
                     /gene="rpmG"
                     /locus_tag="PKV59_01745"
     CDS             complement(6767..6937)
                     /gene="rpmG"
                     /locus_tag="PKV59_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQP45503.1"
                     /translation="MASKKKDVRPVITFACTECKERNYTSQKNRRNDPGRMELMKYCS
                     RCRKHTLHREAK"
BASE COUNT         1805 a         1700 c         1428 g         2097 t
ORIGIN      
        1 tattgttatc gtgctgttat gatgatgcgc tgcctcaggg attgggtgtc tatctgccgg
       61 ataatgaaaa atgggagaat gatcaatggg taacggtcaa agggcattgg gatcgtattc
      121 gcttcggaga aaacagtcgc tatttgatta ttcccgaatc tatttccgaa gccgaaccgc
      181 ctgaagatcc ttatatttac gataaaacag aaccggcgga aacaaattcg gcaaatgaaa
      241 atatctaaac gaaaaattca taaaacaaag atcgcttccg cggaagcgat ctttgcttgt
      301 gacgattcga ttgaaaagac tattctctgg ctcctaattt ctcatccaag atcatcaaac
      361 cgcccggatt atctccgctt ttctctaaca ttgcgataat atattcaaaa ttggcttctt
      421 cttccacctg ttcggaaatg taccaacgta acaattcctg tgtcccataa tctttttctt
      481 ccaaagccag atcgtaaagt ccgtgaatgc tggaagtgat gaacttttcg tgttccaagc
      541 cggctttaaa gacatccaat ggatttttat aatcgacgga aactttttcg attgtgtcta
      601 aggaaacttc gccggatcgc tcataaacat agcggaaaaa tttcatcgca tgactgattt
      661 cttccttcac ttgcttattc atccaggcgg acattccttt gtaattctca ttttcaaagt
      721 atgcttccat gccgagataa atataggcgg aatgaaattc tttattaatc tgttccacta
      781 aagcttgttg catctgtttc gataacatat tttttctcct cagttaattg ttgtttcttt
      841 gtcttccgat tctttctgcc aggaatcaag aagaaataaa ataaactcat tacaacttag
      901 taataattat aacttaaatt aagccaaatg caagataatc ggtaaagaaa gttgctgatg
      961 aacgggaccc tccttacgtg actaaagcaa atattgtgtc gcaaaagaat aaagtgtcga
     1021 aagataatcc gtaattgtct cagggcgacg gaatccgtgt ccttcttctt tatacagccg
     1081 ataaacatgc ggacatttta gacgcgccac taattcgttc gattgagaag gaattaccac
     1141 cggatcggca tccccctgga aaatcgcaag gggtgttttg attttttcga cgtgccgaat
     1201 gggtgaacgt tcttcataaa gcggtgcggc ttccggaagc ggtcccacca gcgaatcggt
     1261 ataatgcaat tccaatttat gagtattaat ggctaaaccg aaaaggtcgg tcaccccata
     1321 aaggcaagca gccgctttga aagaatccgg atattgaatt aatgtattga gaacggtaaa
     1381 accgccggcg cttccgccaa tgatcaagag ccggtttcga tccgccaagt gtagtgcact
     1441 taagcagctc gcaccgagga cggcatcttc tacatcatag acgccccagt tgccatacaa
     1501 actttttaga taaccggttc cataaccgca gctgccgcgg tagtttactt caagatatcc
     1561 gaatccacgg gaagtgaaaa attgtatttc cgggttgaag cgtaaatccg ctttgccggt
     1621 cggtccgccg tgaatatgaa caaaagcagg cggaagtccc acccaatgtg catcggggtt
     1681 ggttggcgga taaaagatac cgtaaactgg agtcccatcc ggggcttccc aagaaatctc
     1741 tcgcggtttt gaaatatatt cttcgtttat cagatcatca cttatccgaa agacagtttt
     1801 gatctcgttt tcatttataa gaataatttg agtaggctgt aacgggctgt cggcaacagc
     1861 tgcgatcatt ccggtctcgg gagaaaccga aatggtgtca aaagaagaat attgcggaga
     1921 atttaatatt cggatttctc cggattcaac cgagacgata caaattttgc ttctggtccc
     1981 aaaaatttgt aaaaatgcga tctgccggct gtccggaaac cagtcataag tctgcactcc
     2041 ctgggcaaaa gccggaaccg tcaaagcaaa gtgatctccg tggaccaaga ttttttcctc
     2101 tccggattcc aaattccgta ggatcagatc ttcccattcg ccgttcgatc gtatgtaaga
     2161 taacattttc ccatcgggtg agaattttgg ctgagaagcg gggaacggca ttttaccggc
     2221 aatatgcacg atttcggaga ttgttccgtt ttccggatcg acttttccga tcatcacttt
     2281 tgaaccgtcc cacggcatat ccggatgatt ccactccacc cacgtaaaat aatctccgcg
     2341 cggactccaa gccggttgca tatagaaatc cgctccgtga acccataaaa acggccagct
     2401 tcgttcgtcc aaacgcagca atgcgatttg atcatccgta ccgtcgccgg atatataagc
     2461 aacaaatcga ccgttcggac tgattaccgg agaagcggtc atataccggt cattggtaag
     2521 cagcttgaat gccggtttat ttaggttctg aagaaataat ccgcgtttgc cggcgctgaa
     2581 taatactttt tcgtttcgta catcaaaatc tccgccgcca taaccgatat ttccccctac
     2641 cggtaaatcg ccgttcagct tttttaaccc cgaatcacag cgatattcat aaagaaatga
     2701 atttcctccc cgattcccgg agaaatagag acggttgtcc gatccccatt ttaaggtatt
     2761 aatcctaccg cgttgagcga ctgcctgctc agaaatggga cttttccaaa gtccgtaagg
     2821 aattggtctc ataggttcct tctgtttcag aatgattttt cgaaaaacga tccttttcaa
     2881 aaaattttga taaagaaatc ataaccgttc agccgttgat tttcaatggc tttgacaaaa
     2941 tcgggttatt aatgattctt ataaaaaacc ggcaggattc ggctgaggaa aaaagatcgg
     3001 cggcacttgc cacagcgggg gcacaccgaa gcgataatcg ggaacaaaaa acatgtttca
     3061 ggtgttcagc ccgaaacatg tttttgattt tcatcgtctt gttgatccgc cggagaaatc
     3121 cgatcactat actccggcga aagaacaaat gattgatgac aaaaaaatta tgaagccggt
     3181 tttacttcaa cggttgcgcc ggccgcttcg agtttgctct ttgcgtcatt tgccgcttct
     3241 ttaccgacag cggaaaggac tttcgcgccg gcggtttcag ccatttgttt cgcttcgact
     3301 aagccgagat ttgtgagaac acggataacc ttaatcgtct cgatcttctt cgggcccgca
     3361 tctttaataa tgacatcaaa ctcggttgct tcttcagccg gtgcagcagc ttcggctacc
     3421 ggtgcagcag ctacggttgc tacaggcgct gcagcggaaa caccccattt gtcttctaac
     3481 gctttaacta attctgaagc ttctaaaaca cttaaagagc ttagcgcttc aacaagtttt
     3541 tcaatatcag ccattttaaa actcctaatt ttattattta tatttttatt tatttcaata
     3601 cgatcaggcg gcagtcggtt cctctttgga aatgtgtgcc tgaatcaccg cggcgagtga
     3661 acgcccgggt tccgcgataa cacgggtcaa ttgtgaagcg ggtgccaaaa tggtaccgag
     3721 caattttgcc cgcataaccg gcagcggcgg taagctcgcg agagctttta cctgactttc
     3781 ggaaatttca ttcttatcta aaaagccgcc ttttatttta aaggcttcat tctttacgag
     3841 atccgaaaag acttttgcca ttgcagcggc atcggtaaaa gcaaacccga ctaatgaagt
     3901 tccggttata tctttctgta acttgtaatt ctccttttcc agcactttct gaatcagggt
     3961 gtcttttaca acatgaactt ctccgccggc ttcgcgtgct tttgcgcgaa tttcatcaat
     4021 tgccggcata tccatctttg aatattcaag cataaatact ccctgacttt tttgaagcca
     4081 ttgggaatat tgctccatca ttttttgctt ttctgattta gtgaatgcca atgaataata
     4141 cctcctccct ttaatttttc aaaaaaaagt ctttgccccg aaactcgaag gcaaagacat
     4201 aaaagtcata aactctataa ctgtcattcg cctcggcagg atattaagcc tttcggcacc
     4261 ggctgtcttc agagaacttt ttttatttta cctgattaat ttgctcttta cttattattc
     4321 cttacaaatt aagcgattgg ttccaatccc tgagccgcag aaagatccac atgaatgccg
     4381 ggtcccattg tcgaagccaa agtaatcttc ttgatataag ttccttttgc tccggccggt
     4441 ctcgcttttc gtacggcatc caagaatgca gccaaattct cgtaaagtgc tttttgatca
     4501 aaagaaactt ttccgatggg ggcgtgaata tttcccgact tgtccagacg gaattccaca
     4561 cgaccggctt tcgcttcttt gatgacacgc ggtaaatctt cggccggaac gacggtaccg
     4621 gctttcggat ttggcattaa accgcgcggt cccaagacac gacctaaacg acccgctttg
     4681 gccatcatgt ccggagttgc gatcgcaaca tcaaaatcgg tgaacccctg ctgaattttc
     4741 tggatccatt catcgctgtc cgcgataata tctgctccac cttcttcggc ttttgcggcg
     4801 ccttctccct gtgcaaagac aaggacgcgt acggtcttgc ctaacccgtt gggaagaacc
     4861 acaacatccc gtacctgttg atccgcctga cgcggatcca aaccggtccg taagtggatc
     4921 tcaacggttg catcaaattt cgtgatgctg gtttctttca ccaaatcaat cgcatctttc
     4981 ggcgcgtaat tggttgatgg atttactttc ttctgtgctt cctgaaattt ctttccgtgt
     5041 tttgccatta taactccttg tggtccgatc gagtatctgt cgtactctcc cacgaaatat
     5101 taatcaacta cctgaattcc catattccga gcggtgcctt caatctgttt catggcacct
     5161 tcaatatcca ccgcattcat atccttcaat ttcgtttcgg cgatttcccg aacctgagca
     5221 cgggtaattt ttccgacccg acctttgctg ccttccggag aagcggtttc gattcccgcc
     5281 gcccgtttaa tcatatagga agccggaggt gtctttagaa caaaagtgaa agaaccgtct
     5341 gtataaacgg aaatttctgc cgggacaatc tcaccgatgc gggtttgggt ccgggcatta
     5401 tattctttca cgaacgccat gatgttgatg ccatggcctg cgagggcggg gcctaccgga
     5461 ggtgccggat tggctttgcc tgcattcaaa tttaatttaa caattgcttt taattttttt
     5521 gccacaatat actccttcgt ggtgctaacg gattttatcc ttccacaaaa ataagggacc
     5581 cggtaaggac cgtttttttg tccctcttta tttcctcaat aaattcttac gctttttcca
     5641 cctgcagaaa atccaactcc acaggggttt ccctgccgaa gaaattgacc attacccgga
     5701 cttttgattt atcctggtca atttcagcaa ccgtcccgcg aaaatcattg aaaggtccgt
     5761 caataatccg gactttttct ccgactttat aggttacgct gaccaccggt gcttcggctt
     5821 ccatccgttt aataatcgaa ttcacttctt caggacgtaa gggagtcggt tggtttccca
     5881 ttccgacaaa tccggtaacg cccggtgtat ttcgaacgat aaaccatgat tcttcggtca
     5941 gaatcatgtt gaccaagaca taacccggga aaatatgacg ttcaatcgtc ttgcgtttcc
     6001 cctctttgac ttcgatttct tcctgagtcg ggataaccac atcaaaaatt ttatccttca
     6061 tccccatgga atcgatccgc tgttcgaggt tatgacggac tttgttttca taaccggaat
     6121 agcaatgaat cacataccaa ttccgttctt cattgctttc gatcttggtt tcttcttcgg
     6181 gtatttgtgc ggatggtcgt tctgttttct tggctttggc gcgagtcgct tttttcgctt
     6241 tcaatatctc ttccgcgccg atatcttcat ctgagttatt cagaacatcc aactcatctt
     6301 tctctttaga gctcattcaa caatcctgtt ccttatccga cgatcgtgcc aattagttca
     6361 gagaacaaaa agtcaacaat tcccaaaaaa accgccatcg caaaaacaac aatcaaaacg
     6421 atttttgtca tatgaagtgc ttcttctctg gtaggccagg ttacttttcg taactcacca
     6481 atcgtctcat tccaccattt agcgatttta ttcggcttct tcggagcgga tggttttgcc
     6541 ggtttttctg ttttctttat ttttgtgtct gccataaaat ggtcactcct tatttcttcg
     6601 gcacttatgc ctataaacgc cgcttgaatg cggcgtcacc ttcttgcagg tgaagcagga
     6661 atcgaaccca caacctcctg ttttggagac aggcgctctg cctaattgag ctattcacct
     6721 aagatacccg accgataaga tacgatcggt cgggtatttc tttttcttat tttgcttccc
     6781 gatgcaaagt atgttttcgg caacgcgaac aatacttcat taattccatt ctcccgggat
     6841 cattgcgacg attcttctgt gaggtatagt ttcgctcttt gcattctgtg catgcaaacg
     6901 taatgacggg gcgaacatct ttctttttgg atgccatatt ctttctcttt cagcgtcaaa
     6961 cgctgatact ccttcttatc cttcgatttc agtaatcaca ccggcgccga cggtcaaccc
     7021 gccttcgcga
//
LOCUS       DAOMIV010000039         6932 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_10992_length_6932_cov_4.571325, whole genome shotgun sequence.
ACCESSION   DAOMIV010000039 DAOMIV010000000
VERSION     DAOMIV010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10992_length_6932_cov_4.571325"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PKV59_01750"
     CDS             complement(<1..275)
                     /locus_tag="PKV59_01750"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45504.1"
                     /translation="MSSSKNLTILYGDDEESIAEYLSRIEQEQIATGLADLNFSKMDG
                     RNFNEGNFFNTVMAMPILSPYRQIILTNPLALASGKDGNKKLLRLLES"
     gene            complement(272..826)
                     /locus_tag="PKV59_01755"
     CDS             complement(272..826)
                     /locus_tag="PKV59_01755"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HQP45505.1"
                     /translation="MEKTIETKVRDLCIQNPVNHVRKNHALEHATVHILEKKFPDKEF
                     AGYSVERGFWIIGKLSIQEIQEAADAAFARLSHGEKDLAIHPGCGTNLATASLLTSVS
                     AVLTFIGTKSEKDRLKRFSSLLLISSMAIQVSKPLGFAIQRNLTTDPDMNGMEISGID
                     TGQIREYPYFFIRTVYQKPCESSL"
     gene            complement(843..2273)
                     /gene="radA"
                     /locus_tag="PKV59_01760"
     CDS             complement(843..2273)
                     /gene="radA"
                     /locus_tag="PKV59_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQP45506.1"
                     /translation="MSKTHTHYVCQQCGRTNPKFLGRCPGCGSYGTMIEEIIPEENPQ
                     SVSGILHNNPLPKALRDIDEKAEPRVDTGIGELSQVLGGGIVPGSVILIGGDPGIGKS
                     TLMLQTALHLSQKGLVLYVSGEESERQIKMRATRLLENNSFIREIPEKLYLVTETNLD
                     VIFSHVQSLKPDYLIIDSIQTVQLPILESTAGSLTQVRECAGKLRELAKTSGITVFLI
                     GHVTKEGMIAGPRVLEHIVDTVLYLEGDRFLSLRLLRSVKNRFGATSEVGVFEMVESG
                     LIEVKNPSELFLAERMVNAPGSAVVATMEGTRPILVEIQALTNPTSFGNPRRTPIGVD
                     MNRLFLISAVISRRIGLRLSEQDIFVNVVGGMHISEPAADLVIAAAIVSSVKNQPIRA
                     DAVMIGEVGLSGELRGSGKLSSRLKEARKLGFTTAVIPKSIHHKEKYPEGMNVKEFRT
                     LREALIFILLPVAEKEGTSSDSDIIN"
     gene            2392..3123
                     /locus_tag="PKV59_01765"
     CDS             2392..3123
                     /locus_tag="PKV59_01765"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HQP45507.1"
                     /translation="MQFVMIFSLIIAVLSVMFALQNTAVVSLKFFIWTFESPLGLLLL
                     IALTVGAIIISILTLPSWFKSKRIKSIHQKELGELEENLAKYRTDLIDTQNKNKDLRQ
                     KVLELEEAKENLEKAHQKTLQEINDLRNALSNAQLTAEEAEVAKKEAIAARDEINIAL
                     KEMDLKIASSKKSAEEAAEITAKEPDTIDEPGMTTAEPVEIPEESLPSSAGNEEPDQP
                     ENSDTDSGTDEFPEEEKKKEKFNLW"
     gene            3117..4160
                     /locus_tag="PKV59_01770"
     CDS             3117..4160
                     /locus_tag="PKV59_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45508.1"
                     /translation="MVTIHFDLPDIIKGVKKTIFSIGFILFIVIFCAGFQAPEGSTEI
                     ESGTNEIPPVTKISWSADSENIFLIGSRSIKRYPVPELDPQSQFYAASADQFIQDYCP
                     GENVIVTLKEDRQLLFYEAGSVKYQSGIKLNYPAMGADCSNDKEILVPSLDEIAVELY
                     DISSGNLMKKLTGFQTAAPVYGATFSPDGKFILWHARASFRLQNIADESWGSPIEFQD
                     FIDVYRLSPDSKELVVAAPSEDYSKNNLIFMDTQSGEELSRIEIGEQPIGAIAYHPNG
                     KFLMAADSTSLYRIDLTDKKVTWKEQMVKSDQNVSDPIRIADISFSPDGKYLAVLQSD
                     EQLSLFRIEEILQ"
     gene            complement(4218..5222)
                     /gene="trpS"
                     /locus_tag="PKV59_01775"
     CDS             complement(4218..5222)
                     /gene="trpS"
                     /locus_tag="PKV59_01775"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQP45509.1"
                     /translation="MENGKIRVFSGARPTGRQHLGNYLGAIQNYVELQKSYECIYSIV
                     DIHALTTVETTKDLKQNCLEMALDWLAAGVDPEKSIMFIQSHVPQVMELHTMLSMVVP
                     MGKLTDLPTFKEKVRMHPQNVNYGLLGYPVLMSADILLYKAELVPVGIDQAPNLEFAR
                     EVVRSFNFRYNTQVLVEPQIKVTTVPKVMGLDGVNKMSKSLNNEIEIAADDETTRKLV
                     MTMVTDPQRIRRTDPGDPDVCNVFSFHKMFSSDEEVEEINRECRIAGIGCVDCKKRLA
                     KNINASLEPLRSRRRKFAEKPDQVWEILYDGADRARKIADQTMLEVREAIGIPIYREF
                     "
     gene            5277..5930
                     /locus_tag="PKV59_01780"
     CDS             5277..5930
                     /locus_tag="PKV59_01780"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HQP45510.1"
                     /translation="MTFKFVVYCNMKIGRYPISKRIFDLIVTLIGMVIALPVMAVVAL
                     LVRMKIGSPVLFKQQRPGLNEKIFTLYKFRTMIDRYDADGKPAADADRLTPFGEKLRS
                     TSLDELPELFCVIKGDMSLVGPRPLLIQYLPLYSDEQRRRHLLPPGITGWAQINGRNA
                     ITWEDKFRLDVWYVDHWSLWLDIKILLRTVGKVLKREGISGQNHVTMEEFKGSKTIL"
     gene            complement(5942..>6932)
                     /locus_tag="PKV59_01785"
     CDS             complement(5942..>6932)
                     /locus_tag="PKV59_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45511.1"
                     /translation="PTKEQLGKMTESANQQTLIASPTEVLPSATPIPDQKKIIIVRIP
                     DANPGILEIFETALRQLPAEIYTIEETDNLTPATVPEDADLIIFPNLPPDLTQYTQQF
                     PQKQFVVLSLQPQTDPNVWTIQYDIGFQPFLAGYFVGVTANDWRGAGLLPSDSPLYGA
                     AIAETFTNGMKLFCGRCQSYGPPYVEFPLTVLLPEDSAPEAWIAALDQIQDSFPNTFY
                     VSEAALSLPLLQKLSEKQAAVITAEKPPEGWNGNWLGSVQINLAGALNDIVRRSANGE
                     QAGAVIPELQIETGIQQSLFNQGKKLYLNKLYADLISGLVSPYDPVPPASFEK"
BASE COUNT         1893 a         1505 c         1546 g         1988 t
ORIGIN      
        1 gactccaata agcggagcag ttttttattt ccgtcttttc cgcttgccag agcaagcggg
       61 ttggttaaaa tgatttgccg atagggtgaa agaatcggca ttgccataac ggtattaaag
      121 aaatttcctt cattgaaatt tctcccgtcc atttttgaaa aattcaaatc cgccaaaccg
      181 gtggcaattt gttcctgctc aatccggctc agatattcgg ctatcgattc ttcgtcatct
      241 ccgtatagaa tagtcagatt ttttgaagaa ctcataaaga ggattcacag ggtttctgat
      301 aaactgtccg aatgaaaaaa taaggatact ctcggatttg tcccgtatcg atgccgctga
      361 tttccattcc gttcatatcg ggatcggtgg ttagattcct ctgaatggca aatcccaacg
      421 gtttggaaac ctgaattgcc atgcttgaaa tcagcaacag agaagaaaat ctttttaacc
      481 tgtctttttc ggattttgtt ccgataaaag ttaaaacagc actgaccgag gttaaaagag
      541 aagcggtcgc caaattggtt ccgcatcccg gatggatagc gagatccttt tctccatgtg
      601 acaatcttgc aaatgccgca tcagccgcct cttgaatttc ctgaatcgat aacttcccga
      661 taatccaaaa acctctttca acggaatatc cggcaaattc tttatcgggg aatttctttt
      721 cgagaatatg aaccgtggca tgctccaaag catgattttt ccgcacatgg tttacaggat
      781 tttgaataca aagatcccga acttttgttt cgatcgtttt ttccataatg ggtcctttat
      841 tttcagttaa taatatccga atcggatgat gttccttcct tttcggcaac gggaagcaaa
      901 ataaatatca aagcttctcg aagtgtccgg aattctttaa cattcattcc ttcggggtat
      961 ttttctttat gatggattga tttcggaata accgctgtcg tgaaacctaa ttttcgggct
     1021 tctttcaagc gtgatgacaa ctttccgcta ccgcgcagtt ctccggaaag gccgacttct
     1081 ccaatcataa cggcatccgc tcgaataggc tgattcttta ccgatgatac aattgcagcg
     1141 gcgatcacca agtcggctgc cggttccgaa atatgcatcc caccgaccac attcacgaat
     1201 atatcctgct cactcaaacg caagcctatt ctgcgtgaaa ttaccgctga aatcaggaac
     1261 aatcgattca tatcaacgcc gatgggagtt cttcgcggat ttccgaaaga agtcgggtta
     1321 gtcaaagctt gaatttcgac gaggatcggt cgagtgcctt ccatcgttgc aacaaccgcc
     1381 gatcccggag cgttgaccat ccgttccgcc aaaaaaagtt cggagggatt tttcacttcg
     1441 attaatccgc tttcaaccat ttcaaaaacg cccacttcgg aagtcgcacc gaagcggttt
     1501 tttacggaac gtaataatct taaagaaaga aagcgatccc cttccaaata taaaaccgta
     1561 tcgacaatat gctccaatac tctcgggcct gcaatcatcc cttcttttgt gacatgtccg
     1621 atcagaaata ccgtaatccc gctggtttta gccaattcgc gtagttttcc ggcacattcc
     1681 cggacctgcg tgagagatcc ggcggttgac tccaaaatcg gaagttgaac cgtctgaatg
     1741 gaatcgataa ttaaataatc cggtttgagt gattgaacat gtgaaaatat gacatccaag
     1801 tttgtttcgg taaccaagta caatttttcg ggtatttcac ggataaagga attattttcc
     1861 aataaacggg ttgcccgcat tttgatctgg cgttcggatt cttctccgga cacatacaat
     1921 accaaccctt tctgggaaag gtgtaatgct gtctgcaaca ttaatgtcga tttcccgatt
     1981 cccggatcac cgccgattaa tatgacggaa ccgggaacga ttcctccgcc taatacctgc
     2041 gaaagttctc cgatgccggt atccacacgc ggttcggctt tttcatcaat atcgcgtaag
     2101 gccttgggaa gcgggttatt gtgaagtatt cccgaaacgc tttgcgggtt ttcttcggga
     2161 atgatctctt ctatcatggt tccgtaactg ccgcaaccgg ggcaccttcc caaaaatttt
     2221 gggttggttc ttccgcattg ttggcaaaca taatgggtat gagtcttcga catggtttaa
     2281 ttataagcat tattctttta tggtatttta ttgccgattc tttcattgag ttttttcgga
     2341 acctgtgaaa gaataaaaaa atcctataca attataaaaa aggagggtcc tatgcaattt
     2401 gtgatgatat tttcactgat tatcgctgtt ctttcggtca tgtttgcttt gcagaatacc
     2461 gctgtcgtga gtctgaaatt ttttatttgg acattcgaaa gtccattggg gcttttactg
     2521 ctgatagctc ttacggtggg tgcgatcatt atttccattc tgactttacc gagttggttt
     2581 aagagtaaac gcattaaatc gattcatcaa aaagaattgg gagaattgga agaaaacttg
     2641 gcgaaatatc ggacggatct gattgacact caaaataaaa ataaagattt acgccaaaaa
     2701 gttttggagt tggaagaagc gaaagagaac cttgagaaag cgcatcaaaa gacgcttcag
     2761 gagatcaatg acttaagaaa tgctctctcg aatgctcaat tgaccgctga agaagctgaa
     2821 gtcgccaaaa aagaagcaat cgcagctcgg gatgaaatca atatagcgct gaaagaaatg
     2881 gatttaaaga ttgcctcgtc caagaaatct gcggaagaag ctgcggaaat tactgcaaaa
     2941 gaacctgata cgatcgacga gccgggaatg acgacagccg agccggttga aataccggag
     3001 gaatcgttac cgagttctgc cgggaatgag gaacccgatc agccggagaa ttccgataca
     3061 gacagcggaa cggatgaatt cccggaagaa gaaaagaaaa aggaaaaatt taatctatgg
     3121 taaccattca tttcgacctt cccgatatta taaaaggagt gaaaaaaaca atcttttcga
     3181 ttgggttcat tcttttcatc gttatttttt gcgccggctt ccaagctccg gaaggatcaa
     3241 ctgaaattga atcgggtaca aacgaaattc ctcccgttac gaaaatatct tggtctgcgg
     3301 attccgaaaa tatctttttg atagggagcc gttccataaa gcgttacccg gttcccgaat
     3361 tggatccgca atctcaattt tatgcggcat cagccgatca gtttattcaa gattattgtc
     3421 cgggagaaaa tgtcattgtg acccttaaag aggaccggca attacttttt tatgaagccg
     3481 gttcggtgaa atatcaatcg ggaattaaat taaactatcc tgcgatgggt gcggattgtt
     3541 caaacgataa ggagattctt gttcccagtt tggatgaaat cgcggtggaa ttgtatgata
     3601 tttcaagcgg aaatctcatg aagaaattaa ccggttttca gaccgcagcc ccggtctacg
     3661 gagcaacttt ttctccggat ggaaaattca ttttatggca cgcccgagcg tcattccgat
     3721 tgcagaatat cgcggatgaa agttggggat ctccgataga atttcaagat tttattgatg
     3781 tataccgtct ttcgcccgat tccaaagaac ttgttgtggc tgctccctcg gaagattatt
     3841 caaaaaataa tctgattttt atggatactc aaagcggaga ggaattgagt cgaattgaaa
     3901 tcggtgaaca accgattggg gcgatcgcat atcatccgaa cgggaaattt ctgatggcag
     3961 cggattccac gagcctttat cgaatagatt taaccgataa aaaggttact tggaaagaac
     4021 agatggttaa atcagaccaa aatgtttcgg atccgatccg tattgcggat atctcatttt
     4081 cacctgatgg gaaatatctc gcggttttgc agtccgatga gcaattgtca ttgttccgga
     4141 tagaggaaat tcttcaataa aatttcaaaa gaagcagctg tttcgatccg aacagctgct
     4201 tctttcattg tgattactca aaattcccga taaatcggga tcccgatggc ttcccgaact
     4261 tccaacattg tttgatctgc gatttttcgc gcccgatctg caccgtcata taaaatttcc
     4321 cagacctgat cgggtttttc cgcaaatttc cttcggcgtg atcgtaaggg ttccagagaa
     4381 gcattgatat tttttgccaa ccgctttttg caatcgacac atccgatacc ggctatccgg
     4441 cattcccgat ttatttcctc aacctcttcg tcagaggaaa acattttatg gaaactgaat
     4501 acattacaca catcgggatc tcccggatct gtccggcgaa ttcgttgcgg gtcggtaacc
     4561 attgtcataa ccaattttcg ggtggtttca tcgtcggctg caatctcgat ttcgttattt
     4621 aagcttttac tcatcttgtt cactccgtct aatcccataa ctttcggtac cgtagtgact
     4681 ttgatttgag gctcaacaag aacttgagtg ttatagcgaa aattgaaaga acgaacaact
     4741 tcgcgggcaa attccaaatt gggtgcctga tcgattccga cgggaaccaa ctccgcttta
     4801 tataacagaa tatccgcact catcagaacc ggatagccta aaagtccata gttcacgttt
     4861 tgcggatgca tacggacctt ttccttgaag gtcggaaggt ctgtcagttt ccccatcggg
     4921 accaccatgg ataacatcgt atgcaattcc ataacctgcg ggacatggga ttggataaac
     4981 atgattgatt tttcgggatc cactcccgca gccaaccaat ccaaagccat ctcgaggcaa
     5041 ttttgtttca gatctttcgt cgtttcaacg gtggttaaag catgaatatc cacaattgag
     5101 taaatacatt cataagattt ttgcaattcg acataatttt gaattgctcc cagataattt
     5161 cccaaatgct gtctgccggt cggacgtgct cccgaaaata cccggatttt cccattctcc
     5221 ataatcaaaa atcccgcttc ctgtagtaag ataagagtca gcaaaaaagt tgattgatga
     5281 cttttaaatt cgtagtctat tgtaatatga aaatcggacg ataccctatt tcaaaaagaa
     5341 tttttgacct gatcgtgacc ctgatcggaa tggttatcgc tttgcccgtc atggcggttg
     5401 ttgcgctctt ggttcggatg aaaatcggtt cgccggtact ttttaaacag caacgcccgg
     5461 gtctgaacga aaaaatcttt acgctttaca aatttcggac aatgatcgat cgctatgatg
     5521 cggatggaaa accggcggcg gacgcagatc gtctgacgcc ttttggagag aaattacgtt
     5581 caaccagtct ggatgagtta cctgagcttt tttgcgtgat taaaggggac atgagtttgg
     5641 tgggtccgcg acccctgttg atacaatatt tgcctcttta ttccgatgaa cagagacgcc
     5701 ggcacttgct tcctcccggg ataaccggtt gggcgcagat caacggtcga aatgccatca
     5761 cctgggaaga taaattccgg cttgatgtct ggtatgtcga tcattggtca ctttggttgg
     5821 atattaaaat tctactcaga actgtcggaa aagtcctcaa gagagaaggg atcagtggtc
     5881 aaaatcatgt aactatggaa gagtttaaag gttcaaagac gattctttag acgatatttt
     5941 attacttttc aaatgaagcc ggcggcaccg gatcataggg gctgaccaat cctgaaatca
     6001 agtcggcata gagtttattc agataaagtt ttttcccctg attgaataaa gactgttgaa
     6061 ttccggtttc gatttgtaat tcgggtataa cagccccagc ctgttcaccg tttgccgatc
     6121 gccggacgat atcattaagc gctcccgcca gattaatttg gacggagccc aaccaatttc
     6181 cgttccaacc ttccggaggt ttttcggcgg ttattaccgc ggcctgtttt tcactcaatt
     6241 tttggagcag cggtaaactg agagctgcct cagaaacata aaatgtgttg ggaaagctgt
     6301 cctgaatttg atctaacgca gcgatccaag cttccggtgc ggaatcctcg ggtaataaga
     6361 cggtcagcgg gaactctacg taaggaggtc cgtaggattg gcatcgcccg cagaataatt
     6421 tcattccgtt ggtaaaagtc tccgctatgg cagctccgta taaaggagaa tcgcttggca
     6481 gtaagccggc tccgcgccaa tcgttggcgg ttacgccgac aaaatatccc gcgaggaacg
     6541 gctgaaagcc gatgtcatat tgaatggtcc atacattcgg atcggtctgc ggctgaaggc
     6601 tcaggacaac aaattgtttt tgcggaaatt gttgagtata ttgagtcaga tcgggtggaa
     6661 ggttcgggaa aatgatcagg tccgcatctt ccgggaccgt tgcaggcgtc agattgtctg
     6721 tttcttcgat ggtataaatc tcggcgggaa gctgccgaag cgcggtttca aaaatttcca
     6781 aaattcccgg attggcatcc ggaattctga cgattattat tttcttctga tccggtatcg
     6841 gtgtagcgga agggaggact tcagtggggg aagcgatcaa tgtctgttgg ttcgccgatt
     6901 cggtcatttt tccgagctgt tcttttgtcg ga
//
LOCUS       DAOMIV010000040         6923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11014_length_6923_cov_5.554164, whole genome shotgun sequence.
ACCESSION   DAOMIV010000040 DAOMIV010000000
VERSION     DAOMIV010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11014_length_6923_cov_5.554164"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..912
                     /gene="pyrH"
                     /locus_tag="PKV59_01790"
     CDS             196..912
                     /gene="pyrH"
                     /locus_tag="PKV59_01790"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQP45512.1"
                     /translation="MTDLKYQRILLKLSGEALSPKEENGIDPQSANKIAQQVKEVFDM
                     GADLAIVIGGGNIWRGKIGIEVGMDQATADYMGMLATVMNSLVLMDALEHIGVTTRVQ
                     TAIEMRSIAEPYIRRRAIRHIEKKRVVIFAGGTGNPYFTTDTAAALRAVEINANLLIK
                     ATKVDGIYDSDPKINPNAVKFDTIRYIDVLSRQLQVMDATAISLCMENKMPLLVLNLW
                     EEGALSKAVRGEPIGTLVTP"
     gene            1076..3805
                     /locus_tag="PKV59_01795"
     CDS             1076..3805
                     /locus_tag="PKV59_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQP45513.1"
                     /translation="MRNIFTILLRFLGLWVFNAICLLACEWVIPGITIIPYQGVPYAV
                     TVMEVSLIFSIMNLFVRPLILLFTLPLNGLTFGLFSLVVNALVLFLASEFTPFFKIEY
                     PIAAFLSGFLFALVNIIITSLIPIDEDFLYYDILVNRFRKSGEKINKDKKGIIVIEID
                     GLAYPRLIKAIEKRKMPYLKELIDSGKFTISEFDCGIPSQTSSCQAGIMYGDNQNICA
                     YRWYDKEQKRIISSSSFQDTNQMEQEILRRKKNGLLDGGMSINNLMSGNADVSLFTVS
                     AIQPKNSEELNNRNINLYFFSLRPYLLTKSILFALADAAVEVIQYVWACLKRKEPRLN
                     RLVHFYPLIRGSTNVLLRDASTALTINEAMRGGPASYTTFYGYDEIAHHSGPDSFEAY
                     HALSGIDHSIKKIHHTAEENSDRDYELFVISDHGQSFGKTFRQRYGYSISDYIRKLAG
                     ECARLKSESQVIGIGNSAADNESSIRAAIYDLTAQKKTQQIPIPKQTLNKLDTVLEEN
                     ADETRAKKRPEDNDIWILASGNLANVYFSFSNQKVDYAEIESHYPNLCYRLIQHPGVG
                     LMVVHQNKTAIAIGKKGSRNLSTGEISGQDPLANYQPTEMRSCQLSYLAEFPNGGDLI
                     LFSTFYPDGTVAAFEELIGSHGGIGGEQNTAFMFHPINVEVPENIFNSKEVYAILKGR
                     RELDAEIDKFSEEKTKQNNGSEDWKWDKLIAGIRDTSKWTKYMREVILFQSSIYKKIS
                     KDTSLNGPAVLIMMMSLLSNMTVAGILFRSLKGLYLGLIIWLVSWIIVIGASYGATLA
                     LRQNIIFSDFLRGMIFCTVFDVLLFGALIPGYENFWIVIILVTRLISVSLAINGICNI
                     QGKRSLLIVPVVLFMLILLVITTYVILESLGYILKIDPLIDFINHINEIVGIS"
     gene            3802..5643
                     /locus_tag="PKV59_01800"
     CDS             3802..5643
                     /locus_tag="PKV59_01800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HQP45514.1"
                     /translation="MTTDIKTIENTIFSKSFYSWSASEIMGLSRKIIKNQSEILANHP
                     ELVQKRIAICGTCNIRFISSGLIPFLMAEGIFPLIFEGKFNGLSSEILSSDSDLYAFD
                     PDYLIAITDYRDIHEFPPYLTSIEKVRETANRRIEESVHFWEYFHQRCRSKVLLTNIA
                     IPNRHILGGLEANLVSGRDVFLRILNLTFLEKKPTFLTFLDQEALSADFGKDRWFDES
                     EWFISKQPFALEAIPIFSLTIAKQIAADIGKIRKCLITDLDNTLWGGILGDDGINGIN
                     LDPNDPIGESFLDFQKTLKLYKDRGILLAVCSKNDPVIAKEVFQKHPNCILKTEDFSA
                     FIADWEDKPINIQKIASQLNIGLDSLVFFDDNPAERDLVKRVLPMVQVIDVPEDPAEF
                     SMALIRSHCFDWGQLTEEDLNRSETLRADRKRSELGAIALNYEDYLKALEMRISIAKA
                     LPYELNRFTQLINKTNQFNLRTRRYNESEITAMATDPDKVLLRVTLQDKFSDYGIISA
                     IILNLDHSRKNILIDTWVMSCRVFKRGIEDEVMNYLIAFAKDNGFQTVEGEYVCSPRN
                     GLVADLYPRYGFKMVQQSDISKKYILDINDYVPCETWFNFELKGKES"
     gene            5640..5888
                     /locus_tag="PKV59_01805"
     CDS             5640..5888
                     /locus_tag="PKV59_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQP45515.1"
                     /translation="MTDLEKIQSIMRDIFDNETLEIDESTSAEDIAEWDSFSNIQLIA
                     AAEQHFAVKFTTQEAIHIRTVGDILNLIRQKKLPVDKL"
     gene            complement(5971..>6923)
                     /locus_tag="PKV59_01810"
     CDS             complement(5971..>6923)
                     /locus_tag="PKV59_01810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQP45516.1"
                     /translation="PAVPSYGAFRRLKKTAADDRPDLIHSWLYHADLYASLVGRKLQI
                     PVIWGLHNATLSPNVKLSTRLVVSQLAHLSQSVPRRIISCSRSGKQNHIDMGYRGDII
                     TVIPNGFDTDRFKPNSEMRRKKRCELGISDDNILIGNISRFDSQKDHLTLIQAWKELL
                     QKVPGIHFRFLLVGSGLNWENKELRDWICDAGLESRMLLLGERDDIPALLNAIDIFTL
                     SSIGEAFPLALGEAMSSGLLCVTTDVGDAPYLLDDCGILVPKNNPAALAEAIRKMVFL
                     SAEEQDVLRFKSRQRIIEHFSILQMGMAYIKVYQECLSIG"
BASE COUNT         2111 a         1391 c         1424 g         1997 t
ORIGIN      
        1 caagcgaaga acattttatc atgaaacgtt aaattcgaca aaaagagagt gttgacggac
       61 tgcgtatcaa atttccatcg tcaaggaaaa aagacaaaaa tttacaaaat caagaaggtt
      121 ttcttaatcc cttataatta ttccagattg cgaaagaaaa gcgaaccatt cagaaaaata
      181 gcgaagaggc ctcaaatgac agacttaaaa taccaacgga tattactaaa actcagcggt
      241 gaagcgcttt cccccaagga agaaaatggg atcgaccccc aatcagccaa taaaattgct
      301 caacaagtca aagaagtttt cgacatgggg gcggatctgg caattgttat cggaggaggg
      361 aatatctggc gtggaaaaat cggaatcgaa gtcggaatgg atcaagcaac cgctgattat
      421 atgggaatgc ttgctaccgt catgaattca cttgtcttaa tggatgcatt ggaacatatc
      481 ggtgttacaa cccgcgtcca aacagccatc gaaatgcgtt cgatcgccga gccgtatatc
      541 cgaagacgcg caatccgaca cattgagaaa aaaagagtag tcatttttgc gggtggcacc
      601 gggaatcctt attttacaac agatacagcc gcagctctga gggctgttga gatcaacgcc
      661 aatctcttga tcaaagccac gaaagtggat ggaatttatg attccgatcc gaaaataaat
      721 ccaaatgctg tcaaattcga taccattcga tatattgatg ttttatcgcg gcagttacag
      781 gtaatggatg cgactgccat ttctctttgt atggaaaata aaatgccgtt attggtacta
      841 aatctttggg aagaaggggc tctcagcaaa gcggtaagag gagaaccgat cgggacactc
      901 gttactcctt aagtgaatct tcttttttgt taaattcgaa attttttaaa cctttctttt
      961 ttccattacg cgaatttgcc tcaatagact tttgaggtaa attccatttc ttcatttccc
     1021 cttccgttat aatagaaaaa gaatgattta ccttttgaaa ggtaaaaata attaaatgcg
     1081 aaatattttt acgattttat tgcggttttt agggttgtgg gttttcaatg caatctgtct
     1141 tttagcttgt gaatgggtta ttccgggtat tacgattatc ccgtatcagg gtgtccctta
     1201 tgcggtaacc gtgatggaag tcagtctgat tttttccata atgaacttat tcgtacgacc
     1261 gttgatttta ttgttcactt taccgcttaa cggactgaca ttcggcttat tttctctggt
     1321 agttaatgct ttggtgctgt tccttgcttc agaatttact cctttcttta aaatcgaata
     1381 ccctatcgct gcttttctgt ccggcttttt attcgccttg gtaaacatca ttatcacatc
     1441 tctgattccg attgatgaag attttcttta ttatgatatt ttggtaaatc gtttcagaaa
     1501 aagcggtgaa aaaattaata aggacaaaaa gggaattatc gtcatcgaaa tcgacggact
     1561 ggcgtaccct cgattaatca aagctattga aaaacggaaa atgccgtatt tgaaagaatt
     1621 gattgattcc ggaaaattta ccatttccga atttgattgc gggatccctt ctcagacttc
     1681 ttcctgccaa gccggtatta tgtatggtga taaccaaaat atctgtgcct atcgttggta
     1741 tgacaaggaa cagaagagaa ttatttcgtc cagcagcttt caagatacaa atcagatgga
     1801 acaggaaatc ctgcgccgca aaaagaacgg tcttttagac ggagggatga gcatcaataa
     1861 cctaatgtcc gggaatgcgg atgtttcctt atttacggtc agtgcaatcc aaccgaaaaa
     1921 ctcggaagaa ttaaataacc gcaacattaa tctttatttt ttttctttaa gaccgtattt
     1981 attgacaaaa tctatcctat tcgccctggc agatgcagcc gttgaagtta tccaatatgt
     2041 ctgggcatgt ctcaaaagaa aagaaccgag acttaatcgt ttggttcatt tttatccgct
     2101 catccgcgga tcaacaaacg tccttttgag agatgcatcc accgcattaa ccatcaatga
     2161 ggcgatgcga gggggaccgg caagctatac cacgttttat ggttatgatg aaatcgcaca
     2221 tcattcaggt ccggatagtt ttgaagcata tcatgcgctg tcgggaattg atcattcgat
     2281 caaaaaaatt catcatactg ccgaagaaaa ctcggacaga gattatgaat tgttcgttat
     2341 ttccgatcac ggtcaatctt tcggaaagac ctttcgccag cgttacggat atagtatcag
     2401 tgattatata cggaaattag ccggtgaatg tgcccgcttg aaatcggaat cgcaagtaat
     2461 cggtatcggg aacagtgcag ctgataatga atccagcatt cgtgcggcga tttatgatct
     2521 gactgctcaa aagaaaacgc aacagatccc gattcccaaa cagacattaa ataaacttga
     2581 tactgttctt gaagaaaatg ccgatgaaac acgagctaaa aaacgaccgg aagacaatga
     2641 tatctggatt cttgccagcg ggaatttagc caatgtttat ttttcatttt caaatcaaaa
     2701 ggtcgattat gccgaaatag aaagccatta tcccaattta tgctatcgcc tcattcaaca
     2761 ccccggagta ggtcttatgg tcgttcatca aaataaaacg gcgattgcaa tcgggaaaaa
     2821 aggcagccgg aatttatcca ccggcgaaat atcaggacaa gatccgttgg caaattatca
     2881 gcccaccgaa atgcgttctt gccaactatc ctatttggct gagtttccaa acggaggaga
     2941 tttaattctt ttttccacct tttatcccga cggaacggtt gctgcctttg aggaattgat
     3001 cggttctcac ggagggattg gcggagagca aaatacagcg tttatgtttc atcccataaa
     3061 tgttgaagta cctgagaata tattcaactc aaaagaagtc tatgccatct tgaagggacg
     3121 tcgtgaatta gatgcggaga tcgataaatt ttcggaagag aaaacgaaac aaaataacgg
     3181 atccgaagat tggaaatggg ataaattaat tgccggcata cgagatactt caaaatggac
     3241 caaatacatg cgggaagtca ttctctttca atcgtcaatt tataagaaaa tcagtaaaga
     3301 tacgagtcta aatggtccgg ctgtgttaat tatgatgatg tcgctgttat caaatatgac
     3361 cgttgccgga attctcttcc gatccttaaa agggctgtat ttggggctga ttatctggtt
     3421 agtctcatgg attattgtta ttggagcttc ctatggggca acgcttgcgt tgaggcagaa
     3481 tatcattttt tccgattttc tgcgcggcat gatattttgt accgtttttg atgtcctttt
     3541 atttggcgct ttgatccccg gctacgaaaa cttctggatt gttattattt tggttacccg
     3601 tctcatctct gtctcgcttg ccattaatgg tatttgcaac atacaaggga aaagatcttt
     3661 attgattgtg cctgttgttt tgtttatgct gatcttgtta gtgattacga cttatgttat
     3721 tttagaaagc ctcggataca ttctcaaaat cgatcccctg attgacttta tcaaccatat
     3781 taacgaaata gtgggaattt catgacaacc gatataaaaa ctattgaaaa tactattttt
     3841 tcgaagtctt tttattcttg gtctgcaagc gagataatgg gattatcgcg aaaaattatt
     3901 aaaaatcaat ctgaaattct ggcaaatcat ccggaactcg ttcaaaaacg aatcgcaatt
     3961 tgcggaacat gcaatattcg gtttatatct tccgggttaa ttccgttttt aatggccgaa
     4021 ggaattttcc cgcttatttt tgaaggaaaa ttcaacggac tgagcagtga aatcctttct
     4081 tccgattcgg acctttatgc ttttgatccg gattatctga ttgcaatcac tgattatcga
     4141 gacattcatg aattcccacc ttacctcact tccattgaaa aagtaagaga gacggcgaat
     4201 cggcggatcg aagagtcggt tcatttctgg gaatattttc atcaacgctg cagaagcaaa
     4261 gtccttttga ccaatattgc cattcccaac cgtcatatac tcggcggact cgaagcgaac
     4321 ctcgtttccg gtcgagatgt atttttaaga attttaaatc tcactttttt agaaaagaaa
     4381 ccgacatttt taacatttct tgatcaggaa gccctttcgg cggatttcgg gaaagaccga
     4441 tggtttgatg aatccgaatg gtttatcagt aaacaaccat ttgcattgga agcaataccg
     4501 attttttcat taacaattgc taaacaaatc gcagccgata tcggaaaaat tcgaaagtgc
     4561 ctcatcacgg atttggataa taccctttgg ggcggaattc ttggcgatga cggtatcaac
     4621 gggatcaacc ttgatccgaa cgatccgatc ggagaatctt ttcttgattt tcaaaaaact
     4681 ctgaagctgt ataaggatag gggaatttta ttggctgttt gcagcaaaaa tgaccctgtt
     4741 atcgccaaag aagtattcca aaaacacccg aattgtattc tcaaaacaga ggatttttcc
     4801 gcattcatcg ccgattggga agataagcca atcaacattc aaaaaatcgc atcgcagctc
     4861 aacatcggac tcgattcact cgtgtttttt gatgataatc ctgccgaaag agatttggta
     4921 aaaagagttt tgccgatggt acaagtcatc gatgttccgg aggacccggc cgaattcagc
     4981 atggcattaa ttcgttccca ttgttttgat tggggacaat tgacggaaga ggatctgaac
     5041 cgttccgaaa ctctccgagc agatcgaaaa aggtcagagc tcggtgccat agcactcaat
     5101 tacgaagatt atttgaaagc cttggaaatg aggataagca ttgcgaaagc cctgccttat
     5161 gagctgaatc gttttacgca attgattaat aagacgaatc aattcaactt gcgaacgcga
     5221 cgttacaacg aatctgaaat caccgccatg gcaaccgatc cggataaagt tctcctgcgc
     5281 gtgacgctgc aagataaatt cagcgattac ggaatcattt cggcaattat tttgaatctt
     5341 gatcactcac ggaagaatat tcttatcgat acgtgggtaa tgagctgccg ggtttttaaa
     5401 cgcggaatcg aagatgaagt gatgaactat ttgatcgctt ttgcaaaaga taacggtttt
     5461 caaacagtcg aaggtgaata tgtctgcagt ccgcgaaacg ggttggttgc cgatctttac
     5521 ccccgctatg gttttaaaat ggtacaacaa agtgacatat cgaaaaaata tatattggac
     5581 atcaatgatt atgttccatg tgaaacatgg ttcaattttg aactcaaagg gaaagaatca
     5641 tgaccgatct cgaaaaaata caaagtatta tgcgcgacat tttcgataat gagacgctcg
     5701 aaattgatga aagtacgagt gcggaagata ttgccgaatg ggattctttt tcaaatattc
     5761 aacttattgc ggctgcggaa caacattttg ccgtgaaatt cacgacacag gaagcaattc
     5821 atattcggac ggtcggagat atattaaatc tgatcagaca aaaaaagctc ccggttgata
     5881 aattataaag atcgatttat atcgcgaata agcgactttt taccgttaat cttcatataa
     5941 atatttccga tcagaaaata tagtaaccgg ttacccgatt gaaagacact cttgatagac
     6001 tttgatatat gccataccca tttgcaaaat cgaaaaatgt tcgataattc tctgcctcga
     6061 tttgaatcgc aaaacatctt gttcttcggc ggacaaaaag accattttcc gaatcgcttc
     6121 ggcaagcgcc gccggattat ttttagggac caaaataccg caatcatcaa gaagataagg
     6181 agcatctccg acatcggtcg taacacacag taatccgctg ctcatcgctt ctcccaatgc
     6241 caaaggaaaa gcttctccga tcgatgacaa tgtaaaaata tctatagcat tcagtaacgc
     6301 cggtatatca tcccgttcac ctaaaaggag catacgggat tccaatccgg catcgcaaat
     6361 ccaatcgcgc aactccttat tctcccaatt taagccggac ccgaccaaca gaaagcgaaa
     6421 atgaatacca ggcaccttct gcagaagttc tttccaagct tggatcaagg ttaaatggtc
     6481 tttttgtgaa tcaaagcggg aaatgtttcc gatcagaata ttgtcatccg atattcccaa
     6541 ttcgcatctt ttctttctcc gcatttccga gttcggttta aaccgatccg tatcaaaccc
     6601 gttgggaatg accgtaataa tatcccctcg ataccccata tcaatatgat tctgctttcc
     6661 actgcgagaa caggaaatga tccggcgagg aaccgattgg gatagatgtg caagctgtga
     6721 aacaaccaac cgggtcgaaa gtttcacgtt cggacttaaa gtggcattat gcagacccca
     6781 tataaccgga atttgcagct ttcgaccgac aagacttgca tacaagtcag catgataaag
     6841 ccaagaatga ataagatcgg gacgatcgtc tgcagctgtt tttttaagac gccgaaaagc
     6901 gccgtaactg ggaacagccg gtt
//
LOCUS       DAOMIV010000041         6797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11289_length_6797_cov_4.661080, whole genome shotgun sequence.
ACCESSION   DAOMIV010000041 DAOMIV010000000
VERSION     DAOMIV010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6797
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11289_length_6797_cov_4.661080"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="PKV59_01815"
     CDS             <1..1061
                     /locus_tag="PKV59_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQP45517.1"
                     /translation="PVDDNSLMEGAIRGMLESLGDPHTRYSDPESYKQESQYIAGEEY
                     EGIGAWVDVSGDYVEITSPMKGSPAEKAGLKPHDLVIAIDGVDMTGVDPKDALNKILG
                     PKGSTVVLTIKRDDQEPFDVSIVRDAVVTPMVASEMRDDGIAYIQLTQFGDLTVEELQ
                     QAFDELLPQNPKGLILDLRNNGGGYVDTCVSVASKFLPKDSLVLIEEAGDGSRTEYHT
                     KEDPVVDPDLPIVVLGNEGTASASEILIGALQYYHRATFVGVQTFGKGSMQIQPELSN
                     GGAVSITIAHWLTPAGKLIHKVGITPDVVVEMSDADVEAGKDPQLDAAAQILLTGKIP
                     EEPSVPQKNTEENGETGL"
     gene            1193..2212
                     /locus_tag="PKV59_01820"
     CDS             1193..2212
                     /locus_tag="PKV59_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQP45518.1"
                     /translation="MEESVSPEEKNAVNEKIGEVQSAVENVADDVAVVTAADQPGDTV
                     TDIDSKGESAGANGPKIKDHFKGKVTKLSLAGALVDIGLDIPAAIHITQIARPEGAEF
                     TNNIHDLLTVGQEVDVWVKKVKDGRIELTMIKPYDLEWREIKKDMVVKGTVVRMEKFG
                     VFVEIGAERPGLIHISELAHSYVRQPSDILKVGDEVEAKVLEVNRRKKQIKLSIKALQ
                     PEPEVEEAPAEKTARPVSARAQQLKEARTTNAVSAPSEEKVRPRQQKKTSKKRAELNS
                     EGITMSFTDENPSGEAEPTAMEIAIREAMEKAKARNKSESKKKSGVSKEQDEILNRTL
                     NNKVG"
     gene            2399..3517
                     /gene="proB"
                     /locus_tag="PKV59_01825"
     CDS             2399..3517
                     /gene="proB"
                     /locus_tag="PKV59_01825"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQP45519.1"
                     /translation="MSYERIVIKVGTSTLTGGSNHLDKPRLVDLARQLSVIAGHGTMV
                     TLVSSGAVAAGRELLNYPHLSKHIPGKQILASVGQPYLMSIYMDLFKIYNLAAAQILL
                     TRNDFIQRKSYLNARNTIEGLLGQHVIPIINENDTVSTDEIRVGDNDMLSAYVSGLIG
                     ADLLVLLTDQDGLFDMNPELHAEAKLIDLVDGVEIPSFVWEAAGGSLTGMGTGGMLTK
                     VRAADVARRMGTPVMIANGTIPDVLLRIAAGERFGTVFRATNTVLESRKRYLLTGFRS
                     NEAAIILDSGATQAIKRGKSLLPAGIIGVQGEFDRGDTVRLVNNDGEEIAVGLVNYSA
                     SEIQKICGAKASEIETILGYTYADEVVHQDNMIKGKTR"
     gene            3514..4779
                     /locus_tag="PKV59_01830"
     CDS             3514..4779
                     /locus_tag="PKV59_01830"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQP45520.1"
                     /translation="MKTILQMGQDARKASKILATAETDRKNAFLLSLADLLESDHTDI
                     LAANAEDVAEAEGVLSPAMVDRLLLNEQRIKGMAKDLRNVAELPDPVGEIFERYTNPD
                     GLKVRKERIPIGVIAVIYESRPNVTVDSAGLMIKSGNAAILRGGKETIRTNQVLISFI
                     QNALSSNGLPADAIQFIDSPDRSFMLELLQSDPYVDMVIPRGGSGLHEFCRKNSRIPV
                     ITGGIGICHLFVDESADLQKSSEVIRNAKVQRPTVCNALETVLIHRSIAEEFIPKLCA
                     KLAADGVSFKVDGNAYNILLLNGDKSIHFEKAGENDFDTEWLSLVLSVKVVDDVKQAI
                     DHISLHGTGHSDGILSENQENTRRFTTEVNSACVYVNASTRFTDGAELGLGAEIAIST
                     QPLHARGPMALRELTTYKWVIEGDYQTRK"
     gene            4793..5113
                     /locus_tag="PKV59_01835"
     CDS             4793..5113
                     /locus_tag="PKV59_01835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQP45521.1"
                     /translation="MISYKFELEKRKAKALNDDGKQIGSCTISICQDYWTIDHTFVDP
                     EFRGQNIASELVKLVVQNAREMGMKILPVCPFAQREFEKKSEYQDVLYRSAVEKPSSS
                     CPVK"
     gene            complement(5176..6213)
                     /gene="queA"
                     /locus_tag="PKV59_01840"
     CDS             complement(5176..6213)
                     /gene="queA"
                     /locus_tag="PKV59_01840"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQP45522.1"
                     /translation="MQTSDFDYYLPPELIAQTPLEPRDSSRLLVYDRKTDEITHCHFY
                     DIGNFLEAGDLLIRNQTRVLPARIYAKKHETGGKAEILLLRRIEPDTWEALVGGKRLH
                     EGTILDLEEGLSAEIVQDLDNSRRLIRFTGPFEDFMQKNGQMPLPPYIHKRLKDPERY
                     QTVYSRQDLAGSAAAPTAGLHFTEELMQKLEARQIHFADVTLHVGLDTFQPVSEADPQ
                     THRIHKEWCELSADTAKKITEARANGHRIVAVGTTSARTLESAAQAALKAGSPDCVIP
                     ISGDTGIYILPGYEFRAVDAMITNFHLPKSTLIMMISAFTSREKILSIYQQAIDMKYR
                     FFSFGDAMLIL"
     gene            complement(6297..>6797)
                     /locus_tag="PKV59_01845"
     CDS             complement(6297..>6797)
                     /locus_tag="PKV59_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HQP45523.1"
                     /translation="RLDYYDQPAMEKIIENAAVKMNVTVDDGGIKEIAVRSRGTPRIG
                     LRLLRRVRDYAQVRADGTINEQVSRDALELMNIDVAGLDDVDRRVLLTIIEKYNGGPV
                     GLSTIAASISEETDTVMEVVEPYLLQLGFIDRTPQGRNATRKAYEHLGIPYNDQNAPK
                     QGSLFE"
BASE COUNT         1898 a         1454 c         1751 g         1694 t
ORIGIN      
        1 aaccggtcga tgataacagc ctgatggagg gcgcgatccg cggaatgttg gaatctttgg
       61 gtgatccgca tacccgctat tctgatcccg aaagctataa acaggaatcg cagtatattg
      121 ccggcgaaga atatgaagga atcggtgcat gggttgatgt gtccggtgat tatgtcgaaa
      181 tcacttctcc gatgaaaggt agtccggcgg agaaagccgg attaaaaccg cacgacttgg
      241 tgattgccat cgacggcgtc gatatgaccg gagtggatcc caaagacgcg ttaaacaaga
      301 tcttaggtcc gaaaggatcg acagttgtat tgaccattaa acgggacgat caggagccct
      361 ttgatgtatc gattgttcgc gacgcggtcg ttacaccgat ggtggcttcc gaaatgcggg
      421 atgatgggat agcctatatt cagttaacgc aattcggtga tttgactgtg gaagaattgc
      481 aacaggcatt tgatgagctg ctgccgcaga atcccaaggg gttgattttg gacctgcgca
      541 acaacggcgg gggatatgta gatacctgcg tatcggtggc ttcgaaattc ttgcccaaag
      601 attcgttggt tttgatcgaa gaggccggag acggatcccg aaccgaatat catacaaagg
      661 aagatcccgt tgtcgatccg gatttgccga ttgttgtttt gggaaacgaa gggacggcgt
      721 ccgcttcgga aattttaatc ggtgctttgc aatattatca tcgggcgaca tttgtcggag
      781 ttcagacgtt tgggaaggga tctatgcaga ttcaacccga gctttcaaac ggcggcgcgg
      841 tcagtatcac gatcgctcat tggctgacac ccgccggaaa gttaatccat aaggttggta
      901 tcactcccga tgttgtggtg gaaatgagcg atgcggatgt ggaagccgga aaggacccgc
      961 aactggatgc ggctgcgcaa attttattaa cgggaaaaat cccggaagag ccgtctgtac
     1021 cccagaaaaa tacggaagaa aacggtgaaa ccggtttata aaaatcgatt tttgccgaat
     1081 tcgggggaat ataatgaatt ttgcacgaaa attgtttttt tgattgacaa gtattaaatt
     1141 atacgagtat aatcaaacgt tgcgaataat aatatctgga gattgggaaa aaatggaaga
     1201 atctgtatca cctgaagaaa aaaatgcagt aaacgaaaaa atcggagagg tacagagcgc
     1261 agtggaaaac gttgcagatg atgttgccgt ggtaacggca gcggatcaac caggagatac
     1321 tgtgactgat atcgactcga aaggagagtc cgcaggcgca aacggtccga aaattaagga
     1381 tcattttaaa gggaaggtta cgaaactgag cctggcaggc gctttggtcg atatcggatt
     1441 agatattccc gctgcaatcc atataaccca gattgcccgt ccggaagggg cggaatttac
     1501 caataatatc catgatttac tgaccgtcgg gcaggaagtg gatgtttggg taaagaaagt
     1561 aaaagacggc cggattgaac tgaccatgat caaaccgtat gatctcgagt ggcgtgagat
     1621 taaaaaagac atggtagtca agggaaccgt tgtccggatg gagaaattcg gtgtttttgt
     1681 agaaatcggt gcggagcgac cgggattgat tcatatcagt gaacttgcgc atagctatgt
     1741 tcgccaacct tcggatatct tgaaggtcgg agatgaagta gaagccaagg ttttggaagt
     1801 caaccggcga aagaagcaga tcaaactcag cattaaagct ttgcaacccg aaccggaggt
     1861 cgaagaggcg ccggcggaga aaacagcccg tccggtcagc gcacgtgcgc aacagttaaa
     1921 agaagccagg accacgaatg ccgtttccgc tccctcggaa gagaaagtta gaccgcggca
     1981 gcagaagaaa acatcgaaga aacgggccga attaaacagc gaaggaatta ccatgagctt
     2041 taccgatgaa aatccgagcg gtgaagcgga accgaccgca atggaaatcg cgattcgaga
     2101 agcgatggaa aaagccaaag cccgaaataa aagcgaaagt aaaaagaaat ccggtgtatc
     2161 caaagagcag gatgaaattc tgaaccgcac tctgaataac aaagtcggat aaaaagtaat
     2221 tcaaagaaat agtaagcttg aggctgatga acgattgtca gcctcttttt ttcaggaaat
     2281 ccccaacgat tgtttttatt gctaaaggaa atatcgaaat gatcggatac aatcagggaa
     2341 taaaccaatc gattaccgtt tttcctgtta cctgtcattt ggtttttggt gatattcaat
     2401 gtcttacgaa cgaattgtta taaaagtcgg aacatcaaca ttaacgggcg gatcgaacca
     2461 tttggataaa ccgcgcttgg ttgatctggc gcggcagctc agtgttatcg caggtcacgg
     2521 aacgatggtt acattggtat cctccggagc ggtggcagcc ggccgtgaat tgctgaacta
     2581 tcctcatctg tcgaagcata ttcccgggaa acagatttta gcctctgtcg gccaacccta
     2641 tttaatgtcc atttatatgg atttgtttaa gatttataat ctggcggcgg cgcaaattct
     2701 gttgacacgg aatgatttca ttcaacggaa aagttattta aacgcccgaa atacaatcga
     2761 aggattattg gggcagcatg taattccgat tattaacgag aacgatacgg tctcgacgga
     2821 tgaaatccgc gtcggcgata acgatatgct ttccgcctac gtttcggggc tgatcggagc
     2881 tgatttattg gttttgttga cggatcagga cggacttttt gacatgaatc cggaacttca
     2941 tgcggaagca aagctcatcg atctcgtgga tggtgtcgaa attccctctt tcgtttggga
     3001 agccgccggc ggcagtttga ccggtatggg gaccggcggg atgctgacga aagttcgggc
     3061 ggcagacgtt gcccgcagga tgggaactcc ggttatgatc gctaacggaa cgattccgga
     3121 tgtgctgctg cgaatcgcag cgggagaacg gtttggcacg gtgtttcgag cgaccaacac
     3181 ggttctcgaa agccgtaaac gatatttgtt gaccggattc cgttcgaatg aagccgcgat
     3241 tattttagat tcgggcgcca cccaggctat caaacgcggg aaaagcttgc ttccggcggg
     3301 aattatcggc gtccaaggtg aatttgaccg aggagatacg gtacggttgg tcaataacga
     3361 cggagaggaa atcgccgtcg gattggtcaa ttactcggct tccgaaattc aaaaaatttg
     3421 cggtgcaaaa gcaagtgaaa tcgaaacgat tctggggtat acctatgctg acgaagtcgt
     3481 tcatcaggat aatatgatca aggggaaaac acgatgaaaa caattcttca aatgggtcag
     3541 gatgcaagaa aagcgagcaa aattcttgcg acggcagaga ccgaccgaaa gaacgctttt
     3601 ttgttaagtc tggcggattt gttggaatcc gatcatacgg acattcttgc ggcaaatgcc
     3661 gaagatgtcg cagaggcgga aggggtttta tctccggcga tggttgatcg attgctgttg
     3721 aatgaacaac ggatcaaagg gatggcaaaa gatcttcgga atgttgccga gctgccggac
     3781 ccggtgggtg aaatcttcga gcgatatacc aatcccgacg gattaaaagt tcggaaagaa
     3841 cggattccga tcggtgtcat tgccgtgatt tatgaatcgc gacccaatgt aaccgtcgac
     3901 tccgccggat tgatgattaa atcggggaat gcggcgattc tgagaggcgg taaagaaacg
     3961 attcgaacca atcaagtgct gatttcattc attcaaaatg ccttgtcctc taacggttta
     4021 cctgccgatg caatccaatt catcgattcc cccgatcgtt ctttcatgtt ggaattgcta
     4081 caatcggatc catatgtgga tatggtgatt ccgcgcggcg ggtccggatt gcatgaattt
     4141 tgtcgaaaaa acagtcgaat tccggtaatt acgggcggca tcggtatttg tcacctgttt
     4201 gtggacgaat ccgcggatct acagaaatcg tcggaggtta tccgcaacgc aaaagttcaa
     4261 cgaccgacgg tttgcaatgc gttagaaacg gttttgattc atcgttcgat tgcggaagaa
     4321 tttatcccga agttgtgcgc caaacttgcg gcagacggcg tatcgtttaa agtcgacggc
     4381 aatgcttata acattttgct tctgaacggt gacaaatcga ttcacttcga aaaagccggt
     4441 gaaaatgatt ttgatacgga atggttatcg ctggttttaa gtgtgaaagt ggtcgatgat
     4501 gtcaaacagg caatcgatca tatatcatta cacggcaccg gacattcgga cggaatctta
     4561 tcggaaaatc aggaaaatac ccgccgcttt acgacagaag tcaattcggc ttgtgtttat
     4621 gtcaatgcca gcacacggtt tactgatgga gccgaattgg gattgggcgc cgaaatcgcg
     4681 atttccactc aacctttgca cgctcgcgga ccgatggcgt tgcgtgaatt aaccacctat
     4741 aaatgggtga ttgaagggga ttatcaaacc agaaagtaag agaaacacga taatgatttc
     4801 gtacaaattc gaattagaaa aacgcaaggc taaagcactc aacgatgacg gaaaacaaat
     4861 cggaagttgc acgatttcca tctgtcagga ttattggacg atcgatcata catttgtcga
     4921 tccggaattc cgcggacaaa atatcgcttc cgaattggtg aaattggtgg ttcaaaacgc
     4981 cagagaaatg ggtatgaaaa tattaccggt ctgtcctttc gcacaacggg aattcgaaaa
     5041 aaagtccgaa tatcaggatg tgctttaccg aagtgccgtt gaaaaaccga gttcaagttg
     5101 tccggtcaag taaagagccg atcaaaatca gatccgtatc aagggaagat tttttccgaa
     5161 aacgattgaa cttttttata aaatcagcat agcgtcgccg aaagagaaaa atcgatattt
     5221 catatcgatt gcctgctgat aaatagataa tattttttca cggcttgtaa atgcactgat
     5281 catcattatc agcgttgatt tcggcagatg aaaattcgta atcattgcat ccacagcccg
     5341 aaattcatac cccggcaaaa tatagatacc ggtatctccg ctgatcggaa tgacacagtc
     5401 cggcgatccc gctttcagtg cggcttgtgc cgcgctttcc agagtccggg cacttgtcgt
     5461 cccgaccgca actatgcgat gaccgttcgc tcgggcttcc gtgatttttt ttgcggtatc
     5521 ggctgacagc tcacaccatt ccttatgaat ccggtgggtt tgcggatctg cttcgctgac
     5581 gggctggaaa gtgtccaaac cgacatgcaa cgtgacatcc gcaaaatgaa tttggcgagc
     5641 ttccaatttt tgcatcagct cttccgtaaa atgtaacccg gcagtcggag cggcagccga
     5701 gccggccaga tcttgtcggg aatataccgt ttgatatctc tccggatcct tcagacgctt
     5761 atgaatatag ggcggcaacg gcatctgtcc gtttttttgc ataaaatcct cgaaaggacc
     5821 ggtaaaacgt atcaaacgac gggaattatc caggtcttga acgatttcgg cgctcagtcc
     5881 ttcctccaaa tcaagaatcg ttccctcatg aagtcttttc ccgcctacca aagcttccca
     5941 tgtgtcgggt tcgatccgtc tcaagagtaa tatttcagct tttccgccgg tttcatgttt
     6001 cttggcatag attctggcgg gcaaaacccg ggtttgattc cggatcaata aatctcccgc
     6061 ctcgaggaaa tttccgatat cataaaaatg gcaatgagtg atttcatctg tttttcggtc
     6121 ataaaccaaa agacgggaag aatcacgcgg ttccaaaggt gtttgcgcaa ttaattcagg
     6181 aggaagatag taatcaaaat ccgatgtctg catatatttt gttttattgt ttctgtgagg
     6241 gtataacgga aaaagtaaat cgaccggtca agattctcgc tattaaattc cgtatgttat
     6301 tcaaataaag atccttgttt gggggcgttc tgatcattat aagggatccc caaatgttcg
     6361 tacgcttttc gcgtggcatt cctgccttgg ggcgtccgat cgatgaaccc caattgcaag
     6421 agataaggtt cgaccacttc catcaccgtg tctgtttctt cgctgatcga ggcagcgatc
     6481 gtactgaggc cgaccggtcc gccgttgtat ttttcaataa ttgtcaacag cacacgccgg
     6541 tcaacatcat ccagaccggc gacgtcaata ttcatcagtt ccaaagcgtc ccgtgaaact
     6601 tgctcgttga tggtgccgtc ggcgcgtact tgcgcatagt cccggacccg tcgcaggaga
     6661 cgcaaaccga ttctgggagt tccgcgcgaa cggacggcaa tctctttgat accgccgtcg
     6721 tcaacggtca cattcatctt aacagccgca ttctcgatga tcttttccat cgccggctga
     6781 tcgtaataat ccaaacg
//
LOCUS       DAOMIV010000042         6766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11351_length_6766_cov_3.895247, whole genome shotgun sequence.
ACCESSION   DAOMIV010000042 DAOMIV010000000
VERSION     DAOMIV010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11351_length_6766_cov_3.895247"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PKV59_01850"
     CDS             <1..250
                     /locus_tag="PKV59_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45524.1"
                     /translation="KHRQARKQFNDYQTDSIMNRVIKSPSLITKMINSGMAIAWFEEM
                     VMSWPGQKFAALQRFFGEYMMAAYAMIELQFYMDDFSF"
     gene            complement(329..2401)
                     /gene="fusA"
                     /locus_tag="PKV59_01855"
     CDS             complement(329..2401)
                     /gene="fusA"
                     /locus_tag="PKV59_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQP45525.1"
                     /translation="MKEYSTDSIRNIALVSHSSAGKTMMTEAFLHFTGATTRLGKIED
                     GTTVSDFDDEEKRRTISLYSTVIPIEYKDKKINVLDTPGYNDFIGEVVSALSVSDGAC
                     VLLDSVSGREVGTEIAWKYVNKFNLPRIVVVNKMNRENADFKKAMASLADYTDMRLIQ
                     VQIPWGEKLDFRGVIDILTMKAYDGAENKIVDIPAEYMEEAQQLRAALTEAAAESDEE
                     LMEKFFESMELSDEEILRGFSKAVKKGDFVPVYCAAGGQEIGVAPILDAFINLMPSPA
                     ERKPIVVKDKKGNDVEISCDDSGPLVAYVWKTTADPFVGKQTFLRVYSGMLTSDSRIW
                     NGTRSVEERYGNLNIPMGKDNIPVKTVHAGDICSVAKLSETVTGDTFSDKANPLTVPV
                     PEYPHALYRVAVYPKTQADSTKISPTLTRLCEEDMTLSWYNEMATNQTILQGMGDQHI
                     DVAIRRAESKFMVNLLTKEPRVPYQETIVKEASAMYRHKKQSGGSGQFGEVHLKVKPN
                     PDAEFEMHNDVFGGAISASYMPAIEKGIRNAMKEGILAGFPIHGIYVSVFDGKEHPVD
                     SKPIAFETAGREAFKLAFADAGPVLEEPIYTVKITVPEEYMGDVMGDLNTRRARILGM
                     ETEKGESTVTALVPLAEMQRYTTQLRSMTGGRGVFTMEFSNYEVVPPHITQEIIANRE
                     KEKKEAEK"
     gene            complement(2624..4018)
                     /locus_tag="PKV59_01860"
     CDS             complement(2624..4018)
                     /locus_tag="PKV59_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HQP45526.1"
                     /translation="MSQNEVQSIGYLPDERPPFFKLVLYALQQVIVMFPATVTVAIIT
                     GFHISTTIFASGLATFCFILVTGRKIPLYYGSSFSYISAIAGIMAAKAAEGADLNTQI
                     QTAQFGIIMSGFVSIFAGLIIKRFGTESIEKVLPATITGPIAMSIGLTLAANALLDAC
                     AVPAGVAEESIDLVRNYAWTISLITLLSTIFFTVYLKGFAGQLTLLFGPIVGCLVTVL
                     ISKFTGFDFFRTLSEGNGTGLFALPVFTLPKPSLAAVAAIMPIAIATIPESTAHLYQL
                     DIYVNNLAKVKKSEKRYNIADKLGLNLMGDGIADIVSALVGGPAGTNYGENISAMTIT
                     RVFSVPVLIAASIIAMIISCFRPLINAIYAIPQPVIGGLEIYLFGAIAAQGIAIMVDK
                     KVDFFDAQSIALIASIMVIGVGGQYAFGGSIPFFGIQVPCIAGAAIFGIVLNLLLSFG
                     KKPALSTQNGTESK"
     gene            complement(4044..4607)
                     /gene="pyrR"
                     /locus_tag="PKV59_01865"
     CDS             complement(4044..4607)
                     /gene="pyrR"
                     /locus_tag="PKV59_01865"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQP45527.1"
                     /translation="MPSIKAVVMDETAMNRALMRISHEIIEHNHGTDKLGLIGIKRRG
                     VPLAKRIAEDIRIIEGVQIPVGSLDITLYRDDLSSIAEMPSVNASEINFSVTGLNIVL
                     VDDVIYTGRTVRAAIDALFALGRPASIQLAVLIDRGHRELPIRPDYVGKNIPTSRNEL
                     IAVNLPEFDNRTNVEIHDSENYSNHGQ"
     gene            5216..6334
                     /locus_tag="PKV59_01870"
     CDS             5216..6334
                     /locus_tag="PKV59_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HQP45528.1"
                     /translation="MKKTLLTVLVLVMLVTSLVGFAPAKEDDFVIGILTGTVSQGEEE
                     FRAAEKMAAKYPGKVITATYPDNFNDEVETTIARALELVSNGADLIVFVQAVPGAAAA
                     IDKVHELKPDFPFIAGVAGEDPDVIASKADVVLGVAEIQMGTKMAEQAAAMGAKTFVH
                     YSFARHMSYATIVARHEILKKRCEELGITFVDATAPDPTGDAGVTGAQQFILEDLPRQ
                     VEKYGKETAFFNTNCGMQPAMIQAVLATGAYYPQQCCPSPYHGYPEALGISIPEDKQG
                     DVSYIIEQIKAKVAEAGMTGHFSTWPVPVNMMMVEAGVEYGFDYLNGEIDNLADVDHW
                     TDLFMDIANGSKVEVFPLELNGVVYDNYLQILSDYITF"
     gene            6599..>6766
                     /locus_tag="PKV59_01875"
     CDS             6599..>6766
                     /locus_tag="PKV59_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP45529.1"
                     /translation="MENINKEYYGNKVLKNVSLNVKRGEIHALVGENGAGKSTLMNIL
                     FGMPVIHSTGGF"
BASE COUNT         1894 a         1548 c         1419 g         1905 t
ORIGIN      
        1 aaagcatcgg caagcgcgta aacaatttaa tgactatcag actgacagta ttatgaatcg
       61 cgttatcaaa agtccgtctc tgattacaaa aatgatcaac agcggaatgg cgatcgcttg
      121 gtttgaagaa atggtcatgt cgtggcccgg gcaaaaattt gccgcacttc agcgattttt
      181 cggagaatat atgatggcgg catatgcaat gatcgaactt caattttata tggatgattt
      241 ttcattttga attgcattcg agtgtgacaa aaaaagcgca acaaatttcg ttgcgctttt
      301 ttgacatgat tgcaattcct caaaagagct atttttccgc ttctttcttt tctttttcgc
      361 gattggcaat gatctcctga gtaatatggg gcggaaccac ttcataattg ctgaattcca
      421 tcgtaaagac accacggccg ccggtcatcg atcgcaactg ggttgtataa cgctgcattt
      481 ccgctaaggg aaccaaagct gtaacggtac tttcgccctt ttcggtttcc attcccaaaa
      541 ttcgggcacg ccgcgtattc aaatcaccca tgacatcccc catgtactct tccggaacgg
      601 ttatttttac cgtatagatc ggttcttcca ataccggacc ggcatcggca aacgctaatt
      661 tgaaggcttc ccgtccagcc gtttcaaaag caatcggttt tgaatcaacc gggtgttcct
      721 tcccgtcaaa tacagaaaca taaataccgt gaatcgggaa accggctaaa attccttctt
      781 tcatcgcatt gcggatgcct ttttcaatcg ccggcatata acttgcgctg atcgcaccgc
      841 cgaaaacatc gttatgcatt tcgaactccg catccggatt gggtttaact ttcaaatgaa
      901 cctcaccgaa ttgaccggaa ccgccggatt gtttcttatg tcgatacatg gcggaggctt
      961 ctttcacgat tgtttcctga taaggcactc tcggctcttt tgtcaaaaga ttcaccataa
     1021 atttggactc tgcccgccga atcgccacat cgatatgctg atcacccatt ccctggagaa
     1081 tggtttgatt ggtcgccatt tcgttatacc atgacaaagt catatcttct tcgcaaaggc
     1141 gagtcaaagt ggggctgatt ttcgtggaat ctgcctgtgt cttcgggtaa acggcgaccc
     1201 ggtaaagggc atgaggatat tcgggaaccg ggacggttaa cggatttgct ttatcgctga
     1261 atgtatcacc ggtcaccgtt tcacttaatt tcgctaccga acaaatatca ccggcatgaa
     1321 ccgtttttac cggaatatta tcctttccca tcgggatgtt caaatttccg tagcgctctt
     1381 caaccgaacg ggtaccgttc catattctgg aatcggaagt aagcattccc gaataaaccc
     1441 gcaaaaaagt ctgcttaccc acaaaaggat cggctgttgt cttccaaaca taggcaacca
     1501 acggtcccga atcatcacaa gaaatttcta catcatttcc ttttttatct tttacgacaa
     1561 tcggttttct ttcggcggga ctcggcatca ggttgataaa ggcatcgaga attggagcca
     1621 ctccaatttc ctgtccgcct gcggcacaat aaaccggaac aaaatcgcct tttttaaccg
     1681 ctttggaaaa tccgcgcaaa atttcttcat ccgaaagttc catgctttca aagaactttt
     1741 ccatcagttc ttcgtcgctt tccgctgccg cttccgtcaa tgcggctcgg agttgttgag
     1801 cttcttccat gtattcggcg ggaatatcga cgattttatt ctccgcgccg tcatatgctt
     1861 tcatcgtcag aatatcgatt acgccgcgaa aatcaagttt ttctccccaa ggaatctgga
     1921 cctgaatcag gcgcatatcc gtataatccg caagagatgc cattgctttt ttaaaatcag
     1981 cgttctcccg attcatctta ttaacgacta cgatccgcgg caaattaaat ttattaacgt
     2041 atttccaagc gatttccgtt ccgacttcac ggcccgatac ggaatccaaa aggacacaag
     2101 cgccgtcgga aacgctcaaa gcggatacga cttcgccgat gaaatcattg tagccggggg
     2161 tgtccagaac attaattttt ttatctttat attcaatagg gattaccgta ctgtataacg
     2221 aaatcgtccg tcgtttttcc tcatcatcaa aatccgaaac ggttgttccg tcttcaatct
     2281 ttcccaatcg ggtggttgct cctgtaaaat gcaggaaagc ttcagtcatc atggtctttc
     2341 cggcgctgct gtgtgaaacc agcgcaatgt ttcgaatcga atcggtcgaa tattctttca
     2401 ttgtttattc cttctgaaat tattcatggc atcttgccaa caatatacag tttagccaat
     2461 caattttatt aggttatcgg ataattgtca aaggacgatt atgattcaag attccatccg
     2521 gtttttatcg ggaaaataaa aaaacacagg catcgatcaa aagcctgtgt ttttcgtttt
     2581 tcttttaata agtttttatg aaaccaaatg catctttacc aatctatttg gattccgtcc
     2641 cattttgggt tgacaatgcc ggttttttcc caaagctcag cagcagattc aaaacgatac
     2701 cgaaaattgc cgctccagca atgcaaggta cctgtattcc gaaaaacgga atactgccgc
     2761 cgaaggcata ctgtccgccg actccgatta ccataatcga tgcgatcagt gcgatactct
     2821 gtgcgtcaaa gaaatcgacc ttcttatcga ccatgatcgc aatcccctgt gcggcgatcg
     2881 ccccgaacaa gtagatctca agtccgccga ttaccggttg cggaatggca tagattgcgt
     2941 tgatcaacgg acggaagcaa gagataatca tggcgataat cgatgcggca attaagaccg
     3001 gaaccgaaaa aacccgtgtg atcgtcatag cgctgatatt ttcaccgtag ttcgtcccgg
     3061 caggaccgcc gacgagcgcc gaaacgatat cggcaattcc gtcacccatc aaattcaagc
     3121 ccagtttgtc cgctatgtta taacgcttct ctgatttttt cacctttgcc aggttattga
     3181 cataaatatc caactgatac agatgagccg ttgattccgg tatggttgcg atcgcgatcg
     3241 gcatgatcgc cgcaaccgcc gccaaagagg gtttgggcag tgtaaaaact ggcagggcaa
     3301 ataatccggt cccgttccct tccgaaagcg ttcggaagaa atcaaagccg gtaaattttg
     3361 aaatcaggac agtgaccagg caaccgacaa tcgggccgaa aagcaaggtt aattgaccgg
     3421 cgaacccttt cagataaacg gtaaaaaata ttgtggagag taaggtaatc aaagaaattg
     3481 tccaggcata attacgtacc aaatcgatcg attcttccgc aactcccgca ggcaccgcac
     3541 acgcatccag caaggcattt gctgccaaag tcaatccgat agacatcgcg atcgggccgg
     3601 tgattgttgc cggtaacact ttttcaatcg attccgttcc gaatcgtttg ataatcaagc
     3661 ccgcaaaaat cgaaacaaaa ccggacatga taattccgaa ctgtgcggtc tggatctgag
     3721 tgttcaaatc cgctccctcc gcagcctttg ccgccataat tccggcgatc gccgagatgt
     3781 aagaaaaact ggatccgtaa tacagcggaa tctttcttcc ggttaccaga ataaaacaaa
     3841 acgtcgcaag accgctggca aaaatggtcg ttgaaatatg aaaacccgtt ataatcgcaa
     3901 cggttaccgt agcgggaaac ataacaataa cttgttgtaa agcatacaaa acaagtttaa
     3961 aaaacggggg acgttcatcc ggcaaatagc cgatcgactg aacttcattt tgcgacatag
     4021 gactctcctc agaaccttga aatttattga ccatgattcg aataattttc ggaatcatgg
     4081 atttcgacat tggttcggtt atcaaattcc ggtaaattaa ccgcaatcaa ctcgttcctg
     4141 gacgtcggta tattcttgcc gacataatcc ggtcgaatcg gtaattcccg atgtccccga
     4201 tcaatcaata cagccagttg gatggaagcc ggtcttccca gcgcgaacaa tgcatcaatg
     4261 gcagctcgca ccgttctgcc cgtataaata acatcatcaa ccaatacaat attcaaaccg
     4321 gtgacggaaa aatttatttc ggaagcgttt accgaaggca tttccgctat agacgacaga
     4381 tcatcgcgat aaagcgttat atccagactg cctaccggaa tctgtacccc ttcgatgatc
     4441 cggatatctt ccgctattct ctttgccaag gggacgccgc gacgtttgat tccgatcaac
     4501 cccaatttat ccgttccgtg gttgtgttca ataatttcat gagaaatccg catcagagcc
     4561 cgattcattg cggtttcatc catgacaaca gctttaatgg atggcatttt tgtattcctt
     4621 tatcagcatt cggattaaaa aaaaacttgt caacgatttt ctcgtttgac aagttttcct
     4681 tttttataat ctttggatca gtcatttttt caccttggcg atctcgctgt atcgcattta
     4741 aagattgaat tggtctgagt atatcatgct ttagaaaagg aaaagcaaaa aaagatgatt
     4801 atcggcagaa aaagttattt atttcctcaa atatcaagcg gtcttacaaa ggactcaaca
     4861 gataatttta tttttttaaa atgggggcgt gtcaaaaatc ataaaaatct gcaacaaatg
     4921 tcatattttc ataaaacagg gcaagaatgt ttccacaaaa tcaagaaata agattattat
     4981 ttaaaacctt aataataagt atttttttaa tttctatccg gcggatttgc tggcggatta
     5041 ttgatcattt gaatattttt ttgtctgatt atagaccctt atcaaccgac aagaagaaga
     5101 caagagctct ggatttctat ttatcttcgt ttttgtcgat caaaactgat tagcggataa
     5161 acatggtgaa atttcaccat ttgatcaaaa taccatcata tttggaggaa acacaatgaa
     5221 aaaaacctta cttactgttt tggtactcgt catgctcgtg acgtcgttag tcggctttgc
     5281 accggcgaaa gaggatgact ttgttatcgg tattcttacc ggtaccgttt cccaagggga
     5341 agaggaattt cgtgccgccg aaaaaatggc tgcaaaatac cccgggaaag tgatcacagc
     5401 aacttaccct gacaacttca atgacgaagt cgaaacaacg attgcacggg ctctcgaatt
     5461 ggtatccaat ggcgcagatc taatcgtgtt tgtccaagct gttccgggtg ccgcagccgc
     5521 catcgataaa gtgcatgaac tcaaacccga ttttccgttt atagccggtg ttgccggtga
     5581 agatcccgat gtcatcgctt ccaaagcgga cgttgttttg ggcgtcgcgg aaattcaaat
     5641 gggcacgaag atggctgaac aggctgccgc gatgggcgca aaaacatttg ttcattactc
     5701 ttttgcacgg catatgagtt atgcgacaat cgtcgcccgg catgaaattt tgaaaaagag
     5761 atgcgaagaa ttggggatca ctttcgtgga tgccaccgct ccggatccga ccggtgacgc
     5821 aggtgttacg ggtgcacaac aatttatcct tgaagactta ccgcgccagg ttgaaaaata
     5881 cggaaaggaa accgctttct tcaacactaa ctgcggaatg cagccggcaa tgattcaggc
     5941 ggttttagct accggtgctt actacccgca acaatgctgc ccctctccgt atcatggtta
     6001 tccggaagcg ctcggaattt ctattccgga agataaacaa ggggatgtat cctatattat
     6061 cgaacagatt aaggcaaaag tggctgaagc ggggatgacc ggtcatttct caacttggcc
     6121 tgtcccggtc aacatgatga tggtcgaagc cggtgtcgaa tatgggttcg attatctgaa
     6181 cggtgaaatc gataatctgg ccgacgtcga tcattggacc gatttattca tggacatcgc
     6241 aaacggttcg aaggttgaag tattcccgct tgaattgaac ggcgttgttt acgacaacta
     6301 tctgcaaatt ctttcggact acattacttt ttaatctgaa tttttctttt cccgagaaaa
     6361 atcgaaggat atgaaaaaat gaaggagagt ctatccaact gatttcatgg ttggatagac
     6421 tcaagtgtga atcggatttt ttccgaaatc ttttttaatg gagataagga tattttgttt
     6481 gttcataaat caaatccttt ttttattctc aaaactaaag aaatgctttg atttatgaat
     6541 tttttcatga cacaaattgc ctcggaggta taaattggat tctgaatatg tcttgaaaat
     6601 ggaaaacatc aataaggagt attatggcaa taaagtgctg aagaatgttt ccctgaatgt
     6661 gaaacgcggt gaaattcatg ctctcgtcgg tgaaaacggt gcgggaaaat ccacccttat
     6721 gaatatcttg ttcgggatgc ccgtaatcca ttcgaccggc ggattt
//
LOCUS       DAOMIV010000043         6716 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11468_length_6716_cov_4.875094, whole genome shotgun sequence.
ACCESSION   DAOMIV010000043 DAOMIV010000000
VERSION     DAOMIV010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6716
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11468_length_6716_cov_4.875094"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="PKV59_01880"
     CDS             <1..803
                     /locus_tag="PKV59_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQP45530.1"
                     /translation="WHGISADLPADDPIDRMDPFLNSIVPADPAEPYSMHQIVETITD
                     RDSFFELQAEFAPNCIIGFARFNGTAVGIVAQEPSIMAGVMDINSSDKISRFVRTCDC
                     YDIPIVTLVDCPGFLPGVYQEHHGVIRHGAKVLYAYAESTVPKVTVVTRKAYGGAYIV
                     LGSKMMGSDVSFAWPQAEIAVMGPEGAAKILYRKQIEAAQDPDAEYRRLVEEYRKNFL
                     NPFVPAETGFIDDVIEPQETRKKIIKALSGIKRKKIVPEERKHGNMPV"
     gene            831..1178
                     /locus_tag="PKV59_01885"
     CDS             831..1178
                     /locus_tag="PKV59_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45531.1"
                     /translation="MEVEIVETTEKLIDNSHVMGQAGQITLIGILILFISLLILWGVM
                     EIIVRVIKDPEKAEVIEEVEAEIITPVVEMVDENVLKMQAAAAAVASIQNYDLKMQVA
                     AAAAGYVAAKKES"
     gene            1237..1644
                     /locus_tag="PKV59_01890"
     CDS             1237..1644
                     /locus_tag="PKV59_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQP45532.1"
                     /translation="MKLTVKVEDKTFDVEIADPNANPMTVKVDGTEFKVYGDSAAKPA
                     PVAHAAKPAAAPAPKPAAAGSDTVKAPIPGVIQEVLVKEGQTVKKGDVLFVLEAMKMK
                     NNILANRDGIVATVNVHKGDSVTHGQVLLSFAD"
     gene            1657..2841
                     /locus_tag="PKV59_01895"
     CDS             1657..2841
                     /locus_tag="PKV59_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQP45533.1"
                     /translation="MGNLGSIVDTFYLLSWREIVMIIVGLVLIGLAIFKEYEPTLLLP
                     IGFGCVLANLGMSAYQTFTVTEVVNGQATEVVRSGFLKVLYDAGITTELFPLLIFVGV
                     GAMIDFRPLLAMPKMVILGAAGQFGIYGTLLLASFLAKSFPGLHWGLAEAASIGTIGA
                     IDGPTAIYVTSKIAPNLLGPIAVAAYSYMSLIPIIQPPIMKALSSKEDRKIRMEYAPR
                     EVSDRSIVVFPMLVTIIISIISPAAAPLIAALMLGNLLKESGVVDRLDKAAQNEIINV
                     STLFLGLAVGGTMIGSQFLTVGVLFILVLGLLAFVLDTVFGMLFGQFMKVITGRKINP
                     LIGACGISAFPMAGRIAAKMALDEDPDNFILMHAMGANTAGQLGSVIAGGVLLALVQG
                     FL"
     gene            3063..4883
                     /gene="glmS"
                     /locus_tag="PKV59_01900"
     CDS             3063..4883
                     /gene="glmS"
                     /locus_tag="PKV59_01900"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQP45534.1"
                     /translation="MCGIVGYAGSKECLPVLLDGLTRLEYRGYDSAGVAVIEQNQIRT
                     VKAKGRLSCLREKLASMEPLTGCCGIGHTRWATHGEPSDVNSHPHSSARVSIVHNGII
                     ENHAEIRDFLIEKGYEFLSETDTEAAVKLLDYYYDGDPIRTILRTTQELHGYYALAIL
                     FLDFPDRIYAVRRESPLVIGYGDGENFVASDIPAFLKYTRRYSLLDTDDIAVITKDGV
                     DIFNSLGNRVKKEILTADWDIESAEKGGYPHFMLKEIHEQPSALEKTLAPRIREGRPD
                     FSGEGIDDAVFREARCIHIIGCGTAMHAGIVAKYAMEKLIHLPVTVDIASEFRYADPI
                     LAQGDPVIVISQSGETLDTIAAMRLAKQAGCTTIAIVNVVGSTIAREADKTVYCWAGP
                     EIAVASTKAYSVQLGLLYLLAIKIAAIRGQISPQRELELTAALFSVPALDEKVLSDTD
                     GIKHIASEYMNADHAFFIGRSLDNAVSVEGSLKLKEISYIHSEAYAAGELKHGTISLI
                     QDGVSVVAVATQYALIEKMFGNIREVKTRGARVLLVTQEDITVPEGLADEIIRIPKVD
                     DFFAPLVAIVPLQLFAYYTAVLRGCDVDKPRNLAKSVTVE"
     gene            5225..5656
                     /gene="ssb"
                     /locus_tag="PKV59_01905"
     CDS             5225..5656
                     /gene="ssb"
                     /locus_tag="PKV59_01905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQP45535.1"
                     /translation="MFHTIIIAGNIGRDPEMRYTPSGVPVTSFSVASSRQYSNTQGQT
                     VKETIWFRVTCWSKLAEYANNYLKKGNKVLIEGRLVPDPNTGGPRVWTRNDGTASASF
                     EVNASTIRSLTTRSESENAGNASGGVPEYSDSEIDEDEIPF"
     gene            5656..6300
                     /locus_tag="PKV59_01910"
     CDS             5656..6300
                     /locus_tag="PKV59_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HQP45536.1"
                     /translation="MNYIDKISEIAGRAYQDFEARTLERDNALTKARSLTRHSANAIR
                     AIHRGEADEAHSQLQEGQKLFEALKAELEIYPDLYYAGYTQDAIKEHCEATLTCAFIK
                     NEPIPTAEEMGVLTATYLRGMAETPGELRRRCMDILRMGYSEEAERLLSLMDEIYSIL
                     ITMDYPDAVTNGLRRQTDLLRGIVERTRADLTLSLREEKLKAELQKAIEVFEKK"
BASE COUNT         1636 a         1506 c         1932 g         1642 t
ORIGIN      
        1 gttggcacgg tatcagcgcc gatcttccgg cggatgaccc gatcgaccgc atggatccct
       61 ttttgaattc gatcgtcccg gctgatcccg ccgaaccgta ttcgatgcat caaatcgtcg
      121 aaacgatcac ggatcgggac agtttcttcg aattgcaggc ggaatttgca ccgaactgca
      181 ttatcggatt tgcccgtttc aacgggacag cggtgggaat tgtggcccag gaaccttcga
      241 taatggccgg ggtgatggat atcaattcat cggataaaat ctcccgcttt gtccgaacct
      301 gcgattgtta tgatatcccg atcgtgacgc tggtcgattg tccggggttt ttacccgggg
      361 tttaccagga acatcatggg gtgatccgtc acggggcgaa ggttctgtat gcttacgccg
      421 aatccactgt acctaaggtg acggtcgtta ctcgcaaagc gtatggtggt gcctacattg
      481 tgttgggcag taagatgatg ggctcggatg tctccttcgc ctggccgcag gctgaaatcg
      541 cggtgatggg tccggaaggg gcagctaaga tattgtatcg aaaacagatc gaagcggcac
      601 aggatccgga cgcggaatat cgccggttgg ttgaagaata tcgaaagaat tttttgaatc
      661 cttttgtgcc ggcggaaact ggttttatcg atgatgtgat cgaaccgcag gaaacacgca
      721 aaaagattat aaaagctttg tccggaataa aaaggaaaaa aatcgtgccg gaagaacgaa
      781 agcacggaaa tatgccggta tagaaaaacc ggcaggaaag gataaaacag atggaagttg
      841 aaatagttga aaccactgaa aagttgatcg acaattccca tgtgatgggt caggcgggtc
      901 agattacgct gatcgggatt ctgatcctgt ttatttcatt attgatcctt tggggcgtaa
      961 tggaaatcat tgtcagggtg atcaaagatc ccgaaaaagc ggaagtgatc gaagaagtcg
     1021 aagcggaaat catcaccccc gtcgttgaaa tggtggatga gaatgtgttg aagatgcagg
     1081 ctgccgccgc cgctgtggct tctattcaga attacgatct gaagatgcag gttgccgccg
     1141 ccgctgccgg ttatgttgcc gccaagaagg aatcataatt gtttttcgaa gaattaaaag
     1201 actgaacgat aaaaaatttt taaaggagtc ttaccaatga aacttactgt taaggtcgaa
     1261 gataaaacat tcgatgtcga aattgccgat ccaaatgcca acccgatgac tgtgaaagtc
     1321 gacgggaccg aattcaaggt ttacggggat tccgctgcca aacccgctcc cgtcgctcac
     1381 gcagcaaaac ccgccgctgc ccccgctccg aagcctgcgg ctgccggctc cgatactgtc
     1441 aaagccccga ttccgggtgt gatccaggag gttttggtca aagagggtca gactgtgaag
     1501 aaaggggatg tcctgttcgt tttggaagcg atgaaaatga agaataacat ccttgccaat
     1561 cgcgacggaa tcgttgcgac ggtcaacgtc cataaaggtg attctgtgac gcacgggcag
     1621 gttttactgt cattcgcaga ttaacgaggt atcgatatgg ggaacctcgg tagtatcgta
     1681 gatacttttt atcttctctc ttggagagaa attgtgatga ttattgtcgg tctggttttg
     1741 atcgggctgg caatcttcaa ggaatatgaa ccgacgttgc tgctgccgat cggtttcggc
     1801 tgcgttttgg cgaacctcgg tatgtcggct tatcagacat ttacggtgac ggaagtggta
     1861 aacggccagg cgacggaagt ggttcgctcg ggtttcctga aggtgttgta tgacgccggg
     1921 attacgacgg aattgttccc gctgctgatt tttgtcggcg tgggcgcgat gatcgatttt
     1981 cgcccgctgt tggcaatgcc gaagatggtt attctgggcg ctgccggcca gttcggaatt
     2041 tacgggacgc ttttactggc ttccttcctt gctaaatctt tcccgggtct ccattggggg
     2101 cttgcggaag ccgcatccat cgggacgatc ggcgccattg acgggccgac ggctatctat
     2161 gtcacatcga agatcgctcc caatttgctg ggaccgattg ccgtggcggc ttattcctat
     2221 atgagcttga ttccgattat ccagcctccc atcatgaagg cgttatccag caaagaagac
     2281 cggaagatcc gcatggaata tgcaccgcgc gaagtttccg atcgttcgat cgtggttttc
     2341 ccgatgctgg ttacgatcat tatcagcatt atttctccgg cggctgcgcc gttgatcgcc
     2401 gctctgatgc tgggtaacct gctgaaggaa agcggtgttg tggatcgttt ggataaagcc
     2461 gctcaaaatg aaattatcaa cgtttccacg ctgtttctcg gcctggcggt cggcggaacc
     2521 atgatcggca gccaattcct gacggtcggt gttctgttca tcctggtact ggggctgctg
     2581 gctttcgtgc tcgatacggt cttcggtatg ctcttcggcc agttcatgaa ggtgatcacc
     2641 ggtcggaaga tcaacccgtt gatcggtgcc tgcggaatca gcgcgttccc gatggcggga
     2701 cggatcgcgg caaagatggc tttggacgaa gatccggaca actttatttt gatgcacgct
     2761 atgggtgcca acacggccgg tcagctgggt tcggtgatcg ccggcggtgt tctgctggct
     2821 ctggtgcagg gttttctcta aaatttttct tttggattag gacgaagacg acttctccgt
     2881 agggggaggt cgtctttttt atgtataatg ggggacttta ttgctcgtga catgaaaaag
     2941 ttgcggggga agccgttccg gcgccgatga gcggggtcag gaaaatcttt cgggatcttg
     3001 aaaatggatt aagattcttc tgtttttgat gtttctgaca acgggtcgga aaggatgaaa
     3061 aaatgtgtgg aattgtcgga tatgccggat ccaaagaatg tttgccggtg ctgttggacg
     3121 gtctaaccag gcttgaatat cgcgggtatg attccgcggg ggtggcagtt atcgaacaga
     3181 atcaaattcg gacggtaaaa gccaagggtc gtttatcttg tctgcgagag aagctggctt
     3241 cgatggaacc gctgaccgga tgctgcggga tcggacatac ccgctgggca acccacggag
     3301 aaccgagcga cgttaattct catccgcatt ccagcgcccg ggtttcgatt gtccataacg
     3361 gaattatcga gaatcatgcc gaaatccgcg attttctgat cgaaaaaggg tatgaatttt
     3421 tgtcggagac ggatacggaa gccgcggtta agctgctgga ttattattat gacggcgacc
     3481 cgattcggac gattcttcga accacgcagg aattgcacgg ttattacgct ctggcgattt
     3541 tattcttgga ttttccggat cgtatttacg ccgttcggcg ggaaagcccg ctggtgatcg
     3601 gatacggaga cggagagaat ttcgtcgctt cggacattcc cgcttttctg aaatataccc
     3661 gccggtacag cttgttggat acggacgata tcgccgtgat caccaaggac ggggtggata
     3721 ttttcaattc actggggaat cgagtgaaaa aagagatatt aaccgccgat tgggacatcg
     3781 agtcggccga gaagggcggt tatccccatt tcatgctgaa ggaaattcat gaacagcctt
     3841 ccgcgctgga aaagacactg gctcctcgca ttcgcgaagg gaggccggat tttagcggcg
     3901 aaggaattga cgacgctgtc ttccgagagg cgcggtgtat tcacatcatc ggctgcggga
     3961 cggccatgca tgccggcatc gtggcaaagt acgcgatgga aaaactgatt catttgccgg
     4021 tgacggtgga tatcgcctcc gagtttcgtt atgccgaccc gattttagcg cagggtgatc
     4081 cggtgatcgt catttcccaa tcgggagaga cgttggatac cattgcggcg atgcggttgg
     4141 cgaaacaggc gggttgcaca accatcgcga tcgtcaatgt ggtgggttcg acgatcgccc
     4201 gcgaggcgga taaaaccgtt tattgctggg cgggtccgga gattgcggtg gcttcgacga
     4261 aagcgtattc ggtacagttg gggctgctct atctgttagc gatcaaaatc gcggcgattc
     4321 gcgggcagat atcaccgcag cgagagcttg agctgaccgc cgcgctgttt tccgtcccgg
     4381 cgctggacga gaaggttcta tcggatacgg atggaattaa gcacattgcc agcgagtata
     4441 tgaatgcgga tcatgccttt ttcatcggcc gttcgctgga taacgccgtc tcggtcgaag
     4501 gctcgctgaa attaaaggag atatcttata tccacagcga ggcatatgcc gccggcgaat
     4561 tgaagcatgg cacgatctca ctgattcagg acggcgtcag cgtggtggcg gtggcgacgc
     4621 aatatgccct gatcgagaag atgttcggca atatccgcga ggtgaaaacc cgcggtgcgc
     4681 gggtgctgct ggtgacccaa gaggatatca cggtgccgga gggattggcg gatgagatca
     4741 tccggattcc caaagtggat gatttttttg cgccgttggt ggctatcgtt ccactccaac
     4801 tgttcgccta ttacacggcg gtattgcgtg gctgcgatgt ggataaaccg cgaaatttgg
     4861 cgaaatccgt gacggtcgaa tgaatgaaaa aagaaatgtc atcaaatttg acgaatgtca
     4921 tatttaggca ttgacaaatc atgacatttg taacataatc aagatcggga cagcaaaagc
     4981 aaaattcttc tttttttatc tttttccaag ccgtctcaga ttgaaaaatt gaaagacggc
     5041 actccttttc atgtaaaggt cgggattgat tcccggcctt ttatgtttaa aaatcacttt
     5101 tcgcgggatt tgcggattaa aacggatctc gggaaatgaa tttcctaaac agaaaaaagt
     5161 tattttgata caattgtcca caacaatgga aaaccgatag aagacaattg aataaggaga
     5221 aaacatgttt catacgatta tcatagcagg gaatatcggc cgggatccgg aaatgcgata
     5281 cacaccgtca ggggttccgg ttacgtcttt cagtgtggct tccagccgac aatattcgaa
     5341 tactcagggt cagacggtaa aggagacaat ttggttcagg gtgacttgct ggtcaaaatt
     5401 ggcggaatat gcaaataact atctgaaaaa aggcaataag gttttgatcg aagggcggct
     5461 ggttccggat cccaataccg ggggtccgcg ggtttggacg cgcaacgacg ggaccgcttc
     5521 ggcttccttc gaagtgaatg cctcgacgat ccgttcgttg acgacccgtt ccgaatcgga
     5581 aaatgccgga aatgcgagcg gcggagtccc cgaatacagc gattcggaaa tcgatgagga
     5641 tgagattccg ttctaatgaa ctacatcgat aaaatttcgg aaatagccgg tcgggcttat
     5701 caggattttg aagcccgtac gctggagcgg gataacgctt tgacgaaagc acgttcgttg
     5761 acacggcatt cggcgaatgc cattcgcgcc attcatcgcg gggaagcgga tgaggcgcac
     5821 agccaattac aggaagggca aaagctgttc gaggcattaa aagccgagct ggaaatatat
     5881 ccggatctgt attatgccgg ctacactcag gatgccatca aggaacattg cgaagcgaca
     5941 ctgacctgtg cgtttatcaa aaacgagccg attcccaccg cggaagagat gggggtgctg
     6001 accgccactt atctgcgcgg gatggccgag acgcccgggg aattgcgccg ccgctgtatg
     6061 gatatcctgc gaatgggata ctcggaagaa gcggaacgat tgctgagcct gatggatgag
     6121 atttattcga tcctgatcac gatggattat cccgatgccg tgacaaacgg attgcggcgg
     6181 cagacggatc ttctgcgcgg tatcgttgaa cggacgcgag cggatttgac tttgagcttg
     6241 cgggaagaaa agctgaaagc ggaactgcaa aaagcgatcg aagttttcga aaaaaaataa
     6301 aacggcatat cgtttacgga tatcctgcac cggggttgag aaattcggtg caggtttttc
     6361 cgattgtgcc gcggaaacgc ctccgcaaga ggaccggtgc gatcgggctt gcttctttcg
     6421 ccgcgccatt cccgacgtac ggagatgaag aaaaacagtc tcagcgcgcc gaaacggatc
     6481 ggctgttcga ctttttctgc gaatgtttcg cgggagtatc gggccggctg cggtaaaatc
     6541 gattgaaaat gaaaaataaa acctcgcaaa aaattatcac cgcttccgag ctggggacct
     6601 atgtctattg tgcgcgggca tggtggtatc ggaatcgaaa cgctcccaac gaaaacctgc
     6661 ccgccatgaa tgaaggcacc cgttttcatc ggaaaatggg caggcgatta tcggcg
//
LOCUS       DAOMIV010000044         6711 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11486_length_6711_cov_4.207031, whole genome shotgun sequence.
ACCESSION   DAOMIV010000044 DAOMIV010000000
VERSION     DAOMIV010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11486_length_6711_cov_4.207031"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..487
                     /locus_tag="PKV59_01915"
     CDS             41..487
                     /locus_tag="PKV59_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45537.1"
                     /translation="MNQEIPGNIALGLNLGGLGGALFFLANIYTILHLIQRIFAPKAE
                     WKWLNNLRNKWHYVHYFGNIAAFVVIVIHAITLWQYATVFNWILIIVMAWMVFAGFTM
                     RFTKAAPQFKKTIRKYHAMWYMLALVLVLIITAHVVSLSSFPYPVG"
     gene            complement(661..1470)
                     /locus_tag="PKV59_01920"
     CDS             complement(661..1470)
                     /locus_tag="PKV59_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQP45538.1"
                     /translation="MNVSKRLDEARKAYDEKNPEASKKIHDEKVIEMAVEQHGSESSQ
                     FIGNMVYGGLDGIISVFAVVSGVAGASLTSGVLLILGAANLLGDGISMGIGAYLSMKS
                     EKEYYRKELERESWEAEHYPEGEKAELLAIYKNKGFPKEDAEKLVEIQSRDHDRWVDA
                     MMINELGMIKDERKPTSAALMTFGSFVLFGLLPLLVYLVDTIFHTNLSMSTMFLISII
                     LSGASLFGLGAAKYFITRRNPLVSGLEMLIVGGLAASVAYFIGVLLKPLGG"
     gene            complement(1722..2123)
                     /locus_tag="PKV59_01925"
     CDS             complement(1722..2123)
                     /locus_tag="PKV59_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HQP45539.1"
                     /translation="MESPRHWRLKKQRYALIGEECPHCKAKIFPPRDVCPECGLEAKT
                     AYQFSGKGKVYSYTTINEAPSGFQDMSPYAIALVKLEEGPIVTAQLTDLGDESVWVGM
                     PVEMVTRKLRNDGDERGVILYGYKFRPAFQA"
     gene            complement(2280..3416)
                     /locus_tag="PKV59_01930"
     CDS             complement(2280..3416)
                     /locus_tag="PKV59_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQP45540.1"
                     /translation="MNRIDHRQRPVILGTGILPIGQHWEISLRQLGGRAVRMALQDAG
                     NPKPDAIYIGNGLGTILSHQANLGALIPEFAGYPGIESCTFEAAGASGAAAFQAACFA
                     VESGYVDVAVALGVEKITECEDRTAEAAMNLALNYEFESSQGLTVKTEAALIAQRYLY
                     EWHLKREIFQNVALNSYQNARGNLNAFARNIDEKAYQKQPMISGPLGLYDIAAQNDGA
                     AAVVLTREELVPAEEQDRLIIVSGSGNAIASLALHDRHDFLHWSAAGQSAATALRQAD
                     LYLSDIDIFEIWDGTVMDVILSLESIGICQEGKGWQIDSLPLNVTGGCLGRGNPLGAS
                     GMYQIAEVARFLQEQKSENGIHRNALIQAIGGYGATAVTHILRS"
     gene            complement(3431..4486)
                     /locus_tag="PKV59_01935"
     CDS             complement(3431..4486)
                     /locus_tag="PKV59_01935"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HQP45541.1"
                     /translation="MKLLIPENPVGIIGYGAYIPRYRIRASEISRVWHGDSEGTPIKE
                     KSVPGMDEDTITMSIEAARNALARAGIPATEIRAVWIGSESHPYAVKPSGTVVAEAIG
                     ADVSVLAADFEFACKAGTEAMIAGMGLVGSGMGKYALAIGMDTAQGKPGDVLEYTAAA
                     GGAAVILGPADEALATIDAAYSFVTDTPDFWRRQSEKYPEHTQRFTGEPAYFNHLEHS
                     IQTFFDETHTKASDYRYAVFHQPNSKFPVKLAKQFGFKKEQYETGLLSPMIGNTYSGA
                     VLLGLSTIFDIAQPGDRILAASFGSGAGSDTFSLVVTDAILSRRNKAPSTQKYIDRRT
                     EIDYAMYARYRNQYLMK"
     gene            complement(4467..5744)
                     /locus_tag="PKV59_01940"
     CDS             complement(4467..5744)
                     /locus_tag="PKV59_01940"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HQP45542.1"
                     /translation="MVKKFYQLTATERIKELIDLKVITEEDAALLLKRGGLSSEVADH
                     MIENGIGIFDLPLGVAQNFMVNGRSVWVPMVIEESSVIAAASNGAKIVLQNGGFTAKA
                     DPPRMIGQIQVINVPDPVPAKRKVLRHKQALIAKLNENRPSLVNRGGGVLDLSGKIFR
                     KSAVGPMLVFYLTIDVRDAMGANLINSALEEIAPDIAKLTAGDIRMCILSNYADKRLV
                     HASCTIRPETLAKEGQAGEEVVERILAANALAEVDPYRAVTHNKGIMNGVDAVVIATG
                     NDWRAVEAGAHSWACKKGKIRPLSSWTKDSDGSLHGELEMPLAVGIVGGATRVHPLAQ
                     FSIRMMQIRTSRELAEIIACVGLAQNLAALRALSTEGIQKGHMALHARQIAITAGAQG
                     ADIDRVAETMCKEGNIAVDHAKEILKRNEITNS"
     gene            complement(5935..>6711)
                     /locus_tag="PKV59_01945"
     CDS             complement(5935..>6711)
                     /locus_tag="PKV59_01945"
                     /inference="COORDINATES: protein motif:HMM:NF025099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HQP45543.1"
                     /translation="GSGTTSKQLAKESDAKPIGYGMMLAESVVALMALSTVMILAKDD
                     PILTKSPNFIYANGLGSFMQLLGINKAFGISFGLMAFTTFVYDTLDVCTRLGRFIYQE
                     LSGMKGVTGRIIGTILIGGIPLILMSINLTDPDGNPVAIWSLFWKTFGSSNQLLAALA
                     LVGITVWLQRTAKHRKAWIATVIPAAFMFVMSTWALVNTFLSYTYVKGAWVMPVGPNK
                     VVPGLSLIYIILAVWVVIECVPAIVKNAHYQDRQPVTQEA"
BASE COUNT         1837 a         1714 c         1353 g         1807 t
ORIGIN      
        1 ggataaataa gaaaattata ttaaaacggc ggctaagatt atgaatcagg aaataccggg
       61 caatatcgca ttgggtctaa atttgggtgg tttgggagga gctttatttt ttctggcaaa
      121 tatctacacc atcttgcatc tcattcaaag aatattcgct ccaaaagccg aatggaaatg
      181 gctgaataat ctaaggaata aatggcatta tgttcattat tttgggaata ttgccgcttt
      241 tgtcgtaatt gttattcatg ccattacatt atggcaatac gcaaccgtct ttaattggat
      301 tctgatcatt gtaatggcat ggatggtatt cgccggcttt accatgcgct ttaccaaagc
      361 ggctccccaa ttcaaaaaga caatcagaaa atatcatgcc atgtggtaca tgctggcact
      421 tgtattggtt ttaatcatta ccgctcacgt cgtctccctt tcctcattcc cttatcctgt
      481 cggataatga agaaaaacgc ccggatgcaa aatggtatcc gggcaaatca gattgaatca
      541 gagcaatata ttttttatca ttcttcgata gaagtttgtc tgtttccgat ccggatagct
      601 attggttcta accgaattga tagctatcta ttctatgaga gatatcatgc acaggcaaaa
      661 ttatccgccc aaaggtttga gtaaaacgcc gataaaataa gccacggaag cagccaagcc
      721 tccgactatc agcatttcca aaccgctgac aagcggattc cggcgagtta taaaatattt
      781 tgccgctcct aaaccgaaaa gcgaagcgcc ggacaaaata attgaaatca gaaacatggt
      841 ggacatggac aaattcgtat gaaaaatcgt atccaccaaa taaaccagaa gcggtaacaa
      901 accgaaaagg acaaatgaac cgaatgtcat caaagctgcg ctggtcggct tccgttcatc
      961 tttaatcatc cccaattcat ttatcatcat cgcatctacc cagcgatcat gatcgcgaga
     1021 ttgaatttcc actaatttct ctgcatcttc cttcggaaaa cccttatttt tataaattgc
     1081 cagtaactcc gctttttctc cttccggata atgttcggct tcccaacttt cgcgctccag
     1141 ctcttttcga taatattctt tttcgctctt catcgaaaga taagcgccga tccccatcga
     1201 aattccgtca ccgagcaaat tagccgcacc cagaatcaac aatacaccgg aagtcaggct
     1261 ggctcccgca accccgctga ctaccgcaaa aaccgagata atcccatcca accctccata
     1321 aaccatgttc ccgatgaatt gggaagattc ggaaccgtgt tgttcgacag ccatctcgat
     1381 gactttttca tcatggattt ttttgctggc ttccggattt ttttcatcat atgcttttct
     1441 ggcttcatcc aatcgttttg atacattcat aacacctccg atctaaattc ttaaaatttt
     1501 tattcataaa accgagactt ttattcatta tacttttttc gtaattcaaa aatttgatcc
     1561 tgatcaattt caagcagaaa ttctaatcct ttaatggctt tatacgaaga ataaaaggga
     1621 aaaaacaacc tgatcgatat tttttcttta aattaaaaaa tcagcgcctc cgttgaatat
     1681 tgacgaaaac gctgactttc cgaattgaat gtttgatttt atcaggcttg aaacgccggt
     1741 cgaaatttgt atccgtatag gataactccc cgttcatcgc cgtcgttacg gagcttccgg
     1801 gtaaccatct caacaggcat tccgacccaa acggattcat cgcccaaatc agtcaattgg
     1861 gctgtaacaa tcggtccttc ttccaattta accaatgcaa ttgcataggg agacatatcc
     1921 tgaaagccgg agggtgcttc attgatcgtg gtatatgagt atacctttcc tttcccgctg
     1981 aattggtaag ctgtttttgc ttccaaaccg cattccgggc agacatcccg tggcgggaat
     2041 atttttgcct tacaatgggg gcattcctct ccgatcaggg catatctctg tttcttcaaa
     2101 cgccaatgac gtggtgattc cattttttct cctcattttt gaaatcttta ttatctgttt
     2161 tcaaaagatt tcagacttca tatcggaagg aatttcgatt gctccttccg catacaacgt
     2221 tcagtagtat aagtaaaaaa gaaaaacaaa aactatcatg atagtttttg atagttcttt
     2281 tatgaccgta aaatgtgtgt gacagcggtt gcgccatatc cgccgatcgc ttggatcaaa
     2341 gcattccgat gaataccgtt ctccgatttt tgttcctgta aaaatcgagc cacctcagca
     2401 atctggtaca tgccggaagc tccgagcgga tttccgcgtc ccaaacaccc gcccgtaaca
     2461 ttcagtggca atgaatcgat ttgccaacct ttcccttctt gacagatgcc gattgattca
     2521 agagataaaa tgacatccat aacggtgcca tcccaaattt caaatatgtc gatatcggaa
     2581 agatacaggt ccgcttgtct taatgcagtg gccgcggatt gaccggcagc cgaccaatgg
     2641 agaaagtcat gacgatcgtg aagtgccaac gatgcgatgg cgtttccgct tccggaaacg
     2701 attatcaatc gatcctgttc ttccgccgga accaattctt ctcgagtcaa tacaaccgcc
     2761 gctgcgccgt cattttgcgc ggcgatatca taaaggccca aaggtccaga gatcatcggt
     2821 tgtttttggt atgctttttc atcgatattt cgagcaaatg cgttgaggtt tcctcgggca
     2881 ttttgatagg aattgagtgc gacattttga aagatctcgc gtttcagatg ccattcatag
     2941 agatatcgtt gggcaatcag tgccgcctcg gtctttaccg tcagcccttg actgctttca
     3001 aattcgtaat tcaacgcaag attcatcgcc gcttccgctg tccgatcctc gcattccgta
     3061 atcttttcca cccccaatgc aaccgcaacg tccacataac cggattcgac cgcaaaacag
     3121 gcggcttgaa aagcggctgc accggaagcg ccggctgcct caaaagtgca gctttcaatc
     3181 ccgggataac cggcaaattc gggaatcaaa gcacccaaat tagcctgatg ggaaagaatg
     3241 gttcccaatc cgttcccgat ataaatcgca tccggcttgg gatttccggc atcctgaagc
     3301 gccatccgaa ccgctcggcc gcccagttgc cggagcgata tttcccaatg ttgcccaatc
     3361 ggtaaaattc cggtgcctaa aataacgggt cgctgtctgt gatcaattct gttcaaaata
     3421 atcttcctct ttatttcatc aaatattgat tccgataacg ggcatacata gcgtaatcga
     3481 tttcggttct tcgatcgata tacttttgcg tgctcggagc tttgtttctt ctggataaaa
     3541 tcgcatcggt tacgacaagt gaaaacgtat cggatcccgc tcccgagcca aaactggcag
     3601 ccaaaattcg atccccgggc tgcgcgatat cgaaaatcgt cgaaagtccc aacaggaccg
     3661 cgcccgaata agtattaccg atcatcgggg acaagagtcc cgtttcatat tgttctttct
     3721 taaagccgaa ttgtttggca agtttaacgg gaaatttact gttcggctga tgaaaaacag
     3781 cgtaacgata atcagaagct ttcgtatggg tttcatcaaa aaaggtttga atcgaatgct
     3841 ccaaatgatt aaaataagcc ggttcaccgg taaaacgctg agtgtgttcc ggatattttt
     3901 cgctttggcg ccgccaaaaa tccggcgtgt cggtcacgaa agaataagcg gcatcaatcg
     3961 ttgccaatgc ttcatccgcc ggtcccaaaa tgaccgccgc accgcccgct gccgcggtat
     4021 attccaagac atcaccgggt tttccttgag cggtatccat tccgattgcc agagcgtact
     4081 ttcccatgcc cgagccgacc aagcccattc cggcaatcat cgcttcggtg ccggctttgc
     4141 aggcaaattc aaaatccgca gccaaaacgg aaacatccgc accgatggct tccgccacca
     4201 ccgtaccgct cggtttgacc gcatacggat gagactccga accgatccat actgcccgta
     4261 tttcggttgc cgggatacct gcccgtgcca acgcattacg ggcagcttca atagacatgg
     4321 taatggtgtc ttcatccatt cccgggacgg atttttcttt aatcggcgtc ccttccgaat
     4381 caccgtgcca tacccgagag atttcggatg cccgaatacg atagcgagga atataggcac
     4441 cgtaaccgat aattccaacc ggattttcag gaattagtaa tttcatttcg tttcaaaatc
     4501 tcctttgcat gatccacagc gatatttccc tctttacaca tcgtttccgc aacccgatcg
     4561 atatccgcgc cttgagctcc ggcagttatg gcaatctggc gggcatgaag tgccatatga
     4621 cctttttgaa taccttccgt tgaaagagca cgtaacgcag ccaaattctg ggctaacccc
     4681 acacaagcaa tgatttcagc caattcacgg gatgttcgga tttgcatcat ccggatacta
     4741 aattgagcca atgggtgaac gcgggtagct ccgccgacaa ttccgacagc caaaggcatc
     4801 tccaattcac cgtgtaagga accgtcggaa tctttcgtcc aagagcttaa cggtcgaatt
     4861 ttaccttttt tgcaagccca actatgcgct cccgcttcta ccgcgcgcca atcattcccg
     4921 gtcgcgataa cgacggcatc cacaccgttc attattcctt tattgtgcgt aaccgcacga
     4981 tatggatcga cttccgccaa ggcattcgcc gctaaaattc gttcaaccac ttcttcgcct
     5041 gcctgccctt ctttggcaag cgtttccggt cgaatcgtac aggatgcatg taccaaccgt
     5101 ttatccgcat aattcgataa aatgcacatc cgaatatctc ccgcggtaag tttcgcaata
     5161 tccggagcga tctcttccaa cgccgaattg atcaggtttg ctcccatggc atcccgcaca
     5221 tcgatggtga gataaaaaac caacatcggt cccaccgcgg attttcgaaa tattttgccg
     5281 gataaatcca aaacgccacc ccctctgttt accagagaag ggcgattttc attcagtttc
     5341 gcgatcaaag cctgtttgtg acgaagaact ttcctctttg ccggcaccgg atcgggaacg
     5401 ttgatcacct gaatttgtcc gatcatccgg ggagggtctg ctttcgccgt gaacccgcca
     5461 ttctgcagca caatttttgc cccgttggaa gccgctgcga taaccgagga ttcttcgatc
     5521 accatcggaa cccaaacaga gcggccgtta accataaaat tttgcgcgac acccaaaggt
     5581 aaatcgaaaa taccgattcc gttttcaatc atgtgatcgg caacttccga agagagcccc
     5641 ccccttttta atagaagcgc cgcatcttct tcagtgatca cctttaaatc aatcagttct
     5701 ttgattcgct cggtagcagt caattgataa aatttcttta ccatttaccc atctttcaat
     5761 ccgaacgccg tctcaaaaga gacggcgttc ttccggttct gtttttattt tatcgaaaaa
     5821 ggctggcaaa aactatcaga aacttacaat tttatcgatt gaaatgatat aagaaaaaaa
     5881 taccgcccga gtcgaaaact caggcggtaa atctatacgg aacaatttta gaaattatgc
     5941 ttcctgtgta accggttgcc ggtcttgata atgggcgttc ttgacaattg ccggtacaca
     6001 ttcaatcaca acccaaacag ccaggataat ataaattaag cttaatcccg gaacaacttt
     6061 attgggaccc accggcatga cccaggcgcc cttgacgtat gtataggaca ggaaagtatt
     6121 aactaaagcc caagtgctca tgacaaacat aaacgcagcc ggaataacgg tcgctatcca
     6181 ggctttccga tgtttggcag ttcgttgtaa ccaaacggtg atacccacca aagccagagc
     6241 agccagtaac tgattcgaag aaccgaatgt cttccagaac aagctccaaa ttgctacggg
     6301 gttcccgtcc ggatccgtca aattaatcga catcaaaatc aacggaatac cgccgattaa
     6361 aatggtaccg attattcttc ccgtcacacc cttcataccg ctcagttcct gataaatgaa
     6421 acgaccgagg cgggtgcaga catccaatgt atcatagaca aacgtcgtga aagccatcag
     6481 cccgaaggaa ataccgaatg ctttattgat tccgagcaac tgcataaagg aacccaaacc
     6541 attcgcataa atgaagttcg gggatttggt taaaatcggg tcatctttcg caagaatcat
     6601 gacggttgac aacgccatca aagcgacaac cgactcagcc agcatcattc cgtaaccgat
     6661 cggtttcgca tcggattctt tagccaactg cttggacgta gtaccggaac c
//
LOCUS       DAOMIV010000045         6676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11564_length_6676_cov_3.611388, whole genome shotgun sequence.
ACCESSION   DAOMIV010000045 DAOMIV010000000
VERSION     DAOMIV010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11564_length_6676_cov_3.611388"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1179)
                     /locus_tag="PKV59_01950"
     CDS             complement(133..1179)
                     /locus_tag="PKV59_01950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQP45544.1"
                     /translation="MKIRSCKYVILALLAFFVTSCNMPERNVDKNVLYTIAASTIQAE
                     NNRIASLTPPTPTSTLTETPTITFTPTITSTPTIPPTATWITHPAGSAEVLILYYNDV
                     ANNANEDPYYQWESPLNVPSVEFEQQVRILYELGYTSIGLSQLVKVLLEGGELPPKPV
                     MFTFDSSKLGQYKNAYPILKKYGMAGNLMLVAAQVDTKNCLSSDQIKEMMNDGWELGS
                     SGYDGNHDPAYYGQEIGKSKILLEEKFGVPIEVFAYPGGIPDSGGVMASKVSSSLYKA
                     AMGKQHVTKQSRDNLYYLGRFEITKGLPLNDFLSYLPWKEGNISEETMNWTLPTVTFT
                     PPAALLQTQETPAP"
     gene            complement(1261..2199)
                     /locus_tag="PKV59_01955"
     CDS             complement(1261..2199)
                     /locus_tag="PKV59_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45545.1"
                     /translation="MMQSKFYRQLQMILKVVPWWKIPISLGVMLSTVEFINFWDVPIN
                     WTAFVLIFLITIISLITANLLQMRLYIFSSDEKSITFLNVPWGKPNEINKNEKDAVSV
                     NLVIYFLFFLLVAVYMSFLFLNKKNQWNLNFVFLLMIISILIFSFAGNNPRINWSEEL
                     FDGLLTVFFPSVLVILGHQNYPFQKYILSYLIPDFCFFLAFRFILGMSMLDYQVSFKP
                     GFFVNYLQKRKFRLHHLFYFLAYFSLILMNRLGVSWELLAPQLLLLPSVSFQIVLIEK
                     IMKGSPVNWKLLGWLSYLNLILLLYFQCITIGFYGF"
     gene            complement(2200..2925)
                     /locus_tag="PKV59_01960"
     CDS             complement(2200..2925)
                     /locus_tag="PKV59_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQP45546.1"
                     /translation="MPESVLITGSVRRLGKMIACHLAESGYHVWLQYLHDDMEAENTC
                     QQIHRFGGAASLIKADLQNVDEIIRMFDFIRENGGLTTLINNAAIFYRSNLATLSVTD
                     WDRLFDINLRAVWLCSREAAKLIGENPTKPGLIINMSDSGSYQYWKEYGGYTLSKAAV
                     RDLTILLAKTFAPKIRVNSISPGLILPEESMDEKTWKHLSSETLLKKSGEPEDILNAV
                     DYLMKSDYITGIDIPVDGGYRWK"
     gene            complement(2918..4093)
                     /locus_tag="PKV59_01965"
     CDS             complement(2918..4093)
                     /locus_tag="PKV59_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQP45547.1"
                     /translation="MKNFSFQIPTQIIFGSGTLEQLNTISLPGKKAMIITGGTSVQKY
                     GYLDQVYKILKYRNIDHVLYDKVVPNPIRKNVMEARDIAREKKCDFFIGLGGGSSIDT
                     AKSVAMMMTNPGDLWDYVPVGQGGKKDFTQKGFPLIAIPTTAGTGTEGNPTSVITNEI
                     TGEKVGIRCEFPIISIVDPDLTININPRYTTYQGFDALFHNIEAFISQKANPFSDLLA
                     LEGVRLIFENLPEAVRNGKNIEARSAVSWAATLGGMVLCLASATSAHVIEHSLSGIDP
                     TVIHGEGLIMISKAFHQLGAERIPELYSKLAFAIGMGDSKKTVQENAKAFLDALEALK
                     IACGVGGLNLSDHGFTEDDIDRIAEGAYVIGGGALTRDQYKITDQDLKEILRNSMHA"
     gene            complement(4118..5662)
                     /gene="gltX"
                     /locus_tag="PKV59_01970"
     CDS             complement(4118..5662)
                     /gene="gltX"
                     /locus_tag="PKV59_01970"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQP45548.1"
                     /translation="MTERKVRVRFAPSPTGALHIGGVRTALYNYLFAKQHGGEFIFRI
                     EDTDSQRFVPGAEEYILETFQWLGIPFNEGVSFGGNYGPYRQSERKDIYKNYVKQLLD
                     NGKAYIAFDTPEQLDAKRAEIPNFQYDASTRLQMTNSLTLSKEEVENRIAAGEQYVVR
                     IKIEPNEEITVHDLIRGDVNVNSSILDDKVLYKSADELPTYHLANIVDDNLMKISHVI
                     RGEEWLPSAPLHVLLYRAFGWEDTMPQFAHLSLLLKPDGNGKLSKRDGDRLGFPVFPL
                     EWRDPKTGDISSGYRESGYLPEAVTNWIALMGWSYDDHTEFFTMEDLIKDFSIDHLNP
                     SPAAINFTKLDYFNGVHIRALPVEDLAHRIKPFFEAKGYHPDDETLLKITPIIQERIV
                     TLDDAPEIAGFFFEDHVTPQTEDLIQKGLTAEKCAEIAEKSYQVLADLPEISPRIGEP
                     EMRALVEELGLKPAQVFGVLRVAVTGRKVSPPLFESMEIIGKDIVLERIKTAAKMLRE
                     YQSRNN"
     gene            complement(5666..6001)
                     /locus_tag="PKV59_01975"
     CDS             complement(5666..6001)
                     /locus_tag="PKV59_01975"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQP45549.1"
                     /translation="MSIIAHLLISALAVYLTAWLLPGISVKSFWTAIGVSIIIAVLNV
                     LVKPLLTLLTIPVTVVTLGLFLFVIDALIILLAGKLLSSFHVDSFWWALLFSIIVTFV
                     TNLLYQLFY"
     gene            complement(6053..>6676)
                     /locus_tag="PKV59_01980"
     CDS             complement(6053..>6676)
                     /locus_tag="PKV59_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HQP45550.1"
                     /translation="FNAEVGDLILILSGSDVNKTRKQLSELRLEMGSQLGLRDKNKYS
                     CLWIVDFPMYEWSDEENRLMAMHHPFTSPKPEDIHLLDTDPANVRANAYDMVINGVEV
                     GGGSIRIHDAKLQAKVFEILGFKPEEAQNKFGFLMNAFKYGAPPHGGLAYGLDRFVSL
                     FVGLDSIRDCIAFPKNNQGRDVMLNAPGFIDQIQLDELGLKVVDKEK"
BASE COUNT         2007 a         1458 c         1240 g         1971 t
ORIGIN      
        1 taagttcccg aatgattgat tcagatccga atcggattaa tttggcgtaa agaaacggta
       61 tttttcaaaa taaaagtgct ccttttatga aatcggagcg ctttttattt tgaaaatgag
      121 tagtatttaa gattacggag ctggggtttc ctgggtttgc agtaaggcag caggcggtgt
      181 gaaagtaacc gtcggcagag tccaattcat tgtctcttca gatatatttc cctctttcca
      241 agggagataa ctcagaaaat catttaatgg cagccctttg gttatttcaa atcggccgag
      301 ataataaaga ttgtcgcgac tttgtttagt cacatgttgc tttcccatcg cagctttata
      361 caaacttgaa ctgactttac tggccataac accgccggaa tctggaattc ctcccggata
      421 agcaaaaact tcaatgggca caccgaattt ttcttcgagc aatattttgg acttaccgat
      481 ttcctgcccg taataagcgg gatcatgatt tccgtcatat ccgctgctgc caagttccca
      541 cccatcattc atcatttctt ttatttggtc agatgaaagg cagttcttcg tatcaacctg
      601 tgctgcgact aacatcagat tccccgccat cccatatttt ttgagaatgg gataagcatt
      661 tttatattgt ccgagcttgg atgaatcaaa agtgaacata accggcttcg gaggaagttc
      721 ccctccttca agcaatactt tgaccaactg cgacagcccg atacttgtat atcccaattc
      781 atacagtatc cggacttgtt gttcaaattc aacggaagga acatttaatg gactttccca
      841 ttgataataa ggatcttcat tcgcattgtt ggcaacatca ttatagtaaa ggattaatac
      901 ttctgcagat ccggccggat gggtaatcca agttgcggtc ggcgggattg tcggagtcga
      961 tgttatcgtc ggcgtaaacg taatcgtcgg ggtttctgtc aaagtcgagg ttggggtcgg
     1021 cggtgtaaga gaagcgatcc tgttattttc ggcttgaatg gttgatgcgg caatggtata
     1081 aagcacattt ttatcgacat tccgctcagg catgttacag gatgtaacaa aaaaagctaa
     1141 taaagctaaa attacatatt tgcaggatct gattttcata attccacctt tttatcgaag
     1201 tgttgtgatc gacattttat aatccgccat ataaaatctt attgatcaat ttctttttaa
     1261 ttaaaatcca taaaaaccaa tcgtaataca ttgaaagtat agcaacagaa taagatttag
     1321 ataagagagc caccctaata atttccaatt tacgggagaa cccttcataa ttttctcaat
     1381 cagaacaatc tggaaggaaa ctgatggaag tagaagtaat tgcggcgcca ataactccca
     1441 acttacacca agccgattca ttagaatcag tgaaaagtat gctaaaaaat aaaataaatg
     1501 atgcagcctg aattttctct tctgcaaata attgacaaaa aagcctggtt taaaggaaac
     1561 ttgataatct agcattgaca tacccaatat gaatcggaat gccagaaaaa agcaaaaatc
     1621 cggaatcaga tagctgagaa tatatttttg aaaaggataa ttttgatggc ccaatatcac
     1681 taagacagat gggaagaaaa cagttaacaa tccgtcgaaa agctcttcgg accagttgat
     1741 tcttggattg tttcccgcaa aagagaaaat taaaatcgaa ataatcatca acaggaaaac
     1801 aaagtttaag ttccattgat tctttttatt caagaaaaga aaagacatat atacagcaac
     1861 taataaaaag aataagaaat atataaccag gttaaccgat acagcatctt tttcattctt
     1921 attgatctca ttcggttttc cccaaggtac atttaagaac gtaattgatt tttcatcgga
     1981 tgaaaaaata taaagccgca tttggagaag attggctgtg attaaagaaa taatcgttat
     2041 taaaaaaatc aaaacaaaag ccgtccaatt tatcggtaca tcccaaaaat taataaattc
     2101 aacggttgaa agcatcaccc ctaaactaat cggtatcttc caccaaggaa caacctttaa
     2161 gatcatttgc aattgtcgat aaaattttga ttgcatcatt tatttccagc gatatccgcc
     2221 gtcaaccgga atatcaatac ctgttatata atccgatttc atcaagtaat cgacagcgtt
     2281 taaaatatct tccggttccc ctgatttttt caataaagtc tcagaggata aatgtttcca
     2341 tgtcttctca tccatcgatt cctcaggcaa aatcagaccg ggagaaattg aattgactcg
     2401 gattttaggc gcaaacgttt ttgcgagaag aatggttaag tcccgtacgg cagctttact
     2461 cagagtatat ccgccatatt ctttccaata ttggtaagat cccgaatcgg acatattgat
     2521 aatcaaaccc ggcttcgtcg ggttttcacc aatcaatttt gccgcctccc tggaacataa
     2581 ccaaacggct ctcaaattaa tgtcgaataa acggtcccaa tctgttacgg ataatgtggc
     2641 taaattgctt cgataaaaaa ttgcggcatt gtttatgagt gttgtcaacc cgccattttc
     2701 acggataaaa tcaaacattc gtataatttc atcgacattt tgaagatccg cttttataag
     2761 tgaagcagcc cctccaaatc gatggatttg ttgacaggtg ttctctgctt ccatgtcatc
     2821 atgtaaatat tgcagccaga catgataacc cgattcagcc aaatggcagg caatcatttt
     2881 accaagtctt cgaacggatc cggtaatcaa aacactttca ggcatgcata gaattccgca
     2941 aaatttcttt taaatcctga tccgtaattt tgtattgatc tctggttaaa gccccacccc
     3001 cgataacata agctccctcg gctatacgat caatatcatc ctcggtaaat ccatggtcag
     3061 ataagtttaa acctcctacg ccgcatgcga tttttaatgc ttcaagtgca tccaagaaag
     3121 ctttggcatt ttcttgaacg gtcttttttg aatcgcccat accgatcgca aaagctaatt
     3181 ttgaataaag ttccggtatt ctctccgctc ctaactgatg aaaagctttt gagatcatga
     3241 tcaacccctc tccgtgaatg acagtcggat caatcccgct caaactgtgt tcgatcacat
     3301 gagccgaagt ggcggaagct aaacaaagta ccattcctcc gagagtcgcc gcccaagaaa
     3361 ccgcagatct ggcttcgata ttctttccgt ttcgaacggc ctcgggtaaa ttttcaaaaa
     3421 ttaagcgaac tccttcaagt gcaagaagat cactgaacgg atttgctttt tgagaaataa
     3481 aagcctctat attatgaaac aatgcatcaa atccctgata tgttgtataa cgcggattaa
     3541 tatttatcgt caaatcggga tcaacgatgg atattatcgg aaactcacag cgtattccga
     3601 ctttttcacc ggttatttcg ttggtaatta cagacgtcgg gtttccttcg gttccggttc
     3661 cggctgtagt cggaatggct attaatggga atcctttttg agtaaaatct tttttgcccc
     3721 cttgtccgac cggtacataa tcccataaat ctcccggatt agtcatcatc atcgcaactg
     3781 attttgccgt atcgatactg cttcctccgc ctaagccgat aaagaaatca cacttttttt
     3841 ctcttgcaat atctctggct tccataacat ttttccgaat aggattcggt acaaccttat
     3901 cgtataacac atggtcaata tttcgatatt ttagaatttt ataaacctga tcaaggtatc
     3961 cgtatttctg tacggaagtc cctccggtaa taatcattgc ttttttcccg ggtaacgaaa
     4021 tcgtattaag ttgttccaat gttccgcttc caaaaataat ttgagtgggt atttgaaaac
     4081 tgaaattctt catttctcac tgctcctttc ttttcggtta attattccgg ctttgatatt
     4141 ctcgtaacat ctttgctgcg gttttgattc tctctaaaac aatgtctttg ccgattattt
     4201 ccatgctctc aaacagagga gggctgactt ttctgcccgt cacggctacg cgcaataccc
     4261 caaaaacctg agccggcttt aaccctaatt cttcaaccaa cgctctcatc tccggttctc
     4321 cgatccgcgg actgatttcc ggaagatcag ccaatacctg ataacttttc tcggctattt
     4381 cagcacattt ttcggcggta agcccctttt gaattaaatc ctctgtctgt ggagtgacat
     4441 gatcttcaaa gaaaaaaccc gctatctcag gcgcatcatc taaagtgacg attcgttctt
     4501 gaataatagg cgtgattttc aacaatgtct cgtcatccgg atgataccct tttgcctcga
     4561 agaacggctt aattcggtga gcgagatctt caaccggtaa tgctcgaata tggacaccat
     4621 taaaataatc gagcttcgta aaattgatcg ctgcggggga aggattcaga tggtctatcg
     4681 aaaaatcctt gatcaagtct tccattgtaa aaaactctgt atgatcgtca taactccacc
     4741 ccatcaaagc gatccaattt gtgacagcct caggcaaata accgctttcg cgatatcctg
     4801 aagaaatatc gcctgtttta gggtcgcgcc attcgagcgg aaacacagga aagccgagac
     4861 ggtcgccatc acgtttactc aatttgccgt ttccgtcagg tttgagcaat agcgataagt
     4921 gtgcaaattg cggcatggta tcttcccacc caaaagcacg atatagcaat acatgcaatg
     4981 gagcagaagg cagccattct tcgccacgaa ttacgtgtga aatcttcatc aaattatcgt
     5041 ccactatatt tgccaaatgg taagtgggca attcatctgc cgatttataa agtactttat
     5101 cgtccaaaat agacgaattg acgtttacat cgccacgaat aaggtcgtgg acggttattt
     5161 cttcattcgg ctcaattttt atgcgcacta catattgttc gcctgccgca atgcgatttt
     5221 ccacctcttc tttactcaac gtaagcgaat tggtcatttg caaacgagta gatgcatcgt
     5281 attgaaaatt aggtatttcc gcacgtttgg catccaattg ttcgggtgtg tcaaaagcaa
     5341 tgtaggcttt tccgttgtcc aacaactgtt taacgtagtt tttgtaaata tctttacgtt
     5401 cgctctgacg atacggtccg taattgccac caaacgaaac gccttcgtta aacggaatac
     5461 cgagccattg aaatgtttct aaaatatatt cttcagcacc cggtacaaaa cgctgactgt
     5521 cagtatcctc aatacgaaaa ataaactcac cgccatgctg tttggcaaac aaatagttgt
     5581 acagggcagt tctcacccct ccaatatgca acgcacccgt aggcgatggc gcaaaacgaa
     5641 cacgaacttt tctttctgtc atattttaat aaaacaattg atataatagg ttagtaacaa
     5701 acgttacaat aatgctaaac aacagagccc accaaaagct atccacatga aaactactca
     5761 acagtttgcc tgccaataaa ataattagcg catcaataac gaacagaaac aagccgagcg
     5821 ttaccaccgt aacgggaatg gtaagcaaag tcagcaaggg ctttaccaat acgttgagta
     5881 cggcaataat gatacttaca ccaatggctg tccaaaaact ttttacacta atgcccggca
     5941 acagccaagc agttaaatac acagccaacg ccgaaatcag aagatgtgca atgatactca
     6001 tatctttttg tttaagtttt taattcaata aatagtaata ttaaaatagt ttttatttct
     6061 ctttatctac aacttttaat cccaattcat ccaattgtat ttggtcaata aatccggggg
     6121 cattaagcat tacatcgcga ccttgattat tttttgggaa ggcgatgcaa tcgcgaatgc
     6181 tgtccaagcc cacaaagagc gacacaaaac ggtcgagacc gtatgccaaa cctccgtggg
     6241 gcggtgcgcc gtacttaaag gcattcatca aaaatccgaa tttattttgc gcttcttcgg
     6301 gcttaaagcc aagaatttca aacacttttg cctgcaattt ggcatcgtga atacggatac
     6361 ttccaccgcc cacttctacg ccgtttatca ccatatcgta ggcattggca cgtacgttgg
     6421 ctgggtcggt atcaagcaga tgaatatctt cgggctttgg cgatgtaaat gggtgatgca
     6481 tagccattaa gcgattttct tcgtcactcc attcgtacat tggaaaatca actatccaca
     6541 gacacgaata tttgtttttg tcgcgcaatc cgagttgtga gcccatttca aggcgtaatt
     6601 cggaaagttg cttgcgggtt ttattaacat cagagccact gagtatgaga attaaatcgc
     6661 cgacttccgc attgaa
//
LOCUS       DAOMIV010000046         6618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11677_length_6618_cov_4.353040, whole genome shotgun sequence.
ACCESSION   DAOMIV010000046 DAOMIV010000000
VERSION     DAOMIV010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11677_length_6618_cov_4.353040"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PKV59_01985"
     CDS             <1..782
                     /locus_tag="PKV59_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HQP45551.1"
                     /translation="GPFPAFLTLSVVLLIQALFFADGGLIALGCNIFNMGVIPCFLIY
                     PLIFQPIIKRGHTTRNFFLASVISSTVNLEAGAFCVVVETLLSGITALPFREFLLLML
                     PIHLPIGLVEGVITASVLIYVFKVRPEILHFLKNEEQPNKVPLRLILASFSIVTILTA
                     GFFSLHASTNPDGLEWSIAQITDSTEFNEEDSLPDKSAEIQHQHSVLPDYNFKEGKTL
                     GNINGTTVAGFLGSGLVFVIAGVSGWLISKLKKRKRESNIE"
     gene            881..1633
                     /gene="cbiQ"
                     /locus_tag="PKV59_01990"
     CDS             881..1633
                     /gene="cbiQ"
                     /locus_tag="PKV59_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HQP45552.1"
                     /translation="MANGNTVIHKLHPYVKSVVLVFYLITVLSVSPYQFDRLAPFILF
                     PAVAISLAEIPFRFIFQRILPVFPFVIFAGLSNLWFDRRIAFFMDSLPITYGAVSFIT
                     LILKTFLTVSAVLILLTTTPMKSLTDLLRSIKIPELIITQIMLSYRYITLLVDEVSVM
                     ETAYLLRSCGKQRIELKHIGSFIGQILLRSYDRATRVAFAMKCRGYGNISNISSSNPL
                     KPVDILIGMGMIGLIFGLRVWNISGVLSRLFL"
     gene            1646..2377
                     /locus_tag="PKV59_01995"
     CDS             1646..2377
                     /locus_tag="PKV59_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45553.1"
                     /translation="MVTIENLSVEYEDGTFALRAINLKVHDGDSIAILGENGAGKTTL
                     FYTMLGLLKISDGKILVNDIELSSPTLKEIRSQIGLVFQNPDDQLFMPTVEEDIAFGL
                     RNYGKSEEEINKKIRDIAERLGIVHLLKKAPYKLSGGEKRLAALATVLAMEPQAILFD
                     EPSSFLDPRSRRTVINLLKEIRDTKMIATHDIDLAWELCDRAIILSKGEVVAEGSIKE
                     ILTNENLLMKYGFELTTCSQTDRRF"
     gene            2503..4230
                     /locus_tag="PKV59_02000"
     CDS             2503..4230
                     /locus_tag="PKV59_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQP45554.1"
                     /translation="MYGIRDAQKEFDRARNQAIWQQALGFLQRKNYNLIDYDEVRKRL
                     VKTVNALPVQKDIPIDAIVGSVSRNNDFTRTFLPKMDYDKNRWVNVRLANESMEGVPP
                     IDVYQIGEVYFVLDGHHRISVMKSYGAQYINANVRIINTEVPLKPTDSPDEIIVKTEK
                     QAFLDQTNIEKLIPEANLEMYLPGEYPELAEHIAVHRYFMGLDYKRDISKDEAVMDWY
                     RTVYMPVIEVIRSQKIMDEFPGKTETELYLWIENNKAALTEEYGEGLRMTALAWKLDE
                     EYGKQKKSIGYRFKKAFFMIFSPDLSDWGIKTGDWRKELRKDDSSLSIQRMMISVRDP
                     IEDFDYLRSAVYFCKQFNAWAGIVHVVKRPSMLDSEWIRKYEQDIQTLLEKENVKGKF
                     FVLKGNLLKNLSDRAFWSDISLFKMNYRPSPSRYRSLGSGWNSIFMRVPGPIFVTPDL
                     FPSKIQQILLAFSQSPKSREAMYFASGLAQSTGGHITVVVSGGDEQRRAVTRQEVENY
                     YQAQGIQAEYVTSSDEPGKAIIENANRKKSDLIIMGGYSRSYIKRLFTGSTIEKVLSV
                     TSIPVIICK"
     gene            complement(4234..5127)
                     /locus_tag="PKV59_02005"
     CDS             complement(4234..5127)
                     /locus_tag="PKV59_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQP45555.1"
                     /translation="MKYYGAIEAGGTKFVCSILADPDHYFEEVRFPTTTPEETLERTV
                     SFFKEQRNKYALTAIGVSCFGPIDLNPSSPTYGYITTTPKIKWRYTNIVGFLKQELNL
                     PVGFDTDVNGAALAEYKWGNPENIRNLVYYTVGTGVGGGVVIDGKPVHGLVHPEAGHI
                     LIRQNLEKDPFKGDCPYHGNCLEGLCAGPTIQARFGIPPQDLPEDHPFWPILANYLAQ
                     ACIAQIYQISPQKIVLGGGVMGQKQLYPMIRKEVIRLLNGYIQHPMILEHIDQYITPP
                     ILGNRAGACGAMALAQMLEEQ"
     gene            complement(5131..6384)
                     /gene="larA"
                     /locus_tag="PKV59_02010"
     CDS             complement(5131..6384)
                     /gene="larA"
                     /locus_tag="PKV59_02010"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HQP45556.1"
                     /translation="MNISFGFGQSTVQLNIPDKNLLKVLEANPIQMIGDESEIVQKAL
                     QNPIQSRRLNEIVRKGEKIAIITSDITRPMPSARVLPFVLDELLSAGIPPSDIVIIFA
                     LGNHRSHTEEEKKMLVGEEIFKKYECIDSDGPMVRLGITANGTPVDIFERVVKVDKRI
                     CLGNIEYHYFAGYSGGAKAIMPGVSTREAIQANHRHMTDPNAIAGKIENNPVRTDIES
                     VLNFISVDFIVNVILDEHKKIVRCVAGDVIAAHRQGCRYLDQMYSVEIPEKADIVLVS
                     PGGYPKDINLYQAQKALDNAKNAVRNGGSILWIASAKEGLGEKTFEEWMLHHNNPSEM
                     IDHIQKEFVLGGHKAAAIAMVMQKATVILYSELNPDLVRKIKLESTQNPQETLERLIN
                     QYGQNASIIAMPYGGATLPILADKR"
     gene            complement(6409..>6618)
                     /locus_tag="PKV59_02015"
     CDS             complement(6409..>6618)
                     /locus_tag="PKV59_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQP45557.1"
                     /translation="DFGVVGFDDVYFASLPQIQMTTIAQPRNQMGVLAAEILIRHIES
                     GDNEEEHILLQPELIIRNSTRKPLR"
BASE COUNT         1863 a         1284 c         1432 g         2039 t
ORIGIN      
        1 tgggtccttt ccccgcattt ctgactcttt ccgtggttct gttgattcaa gctttatttt
       61 ttgccgacgg tggtttaatc gctttaggat gtaatatttt taatatgggt gttattccgt
      121 gctttttgat ataccctttg atatttcaac cgatcataaa aagaggacat acaacgagga
      181 atttctttct tgcttcggtc atttcttcaa cagtcaattt ggaagcgggt gcgttttgcg
      241 tcgtggttga gacattgtta tccggaatta cagctttgcc gttcagagaa tttttgttgt
      301 tgatgttacc cattcatctt cccatcggtc ttgtcgaggg tgtcattacc gcttccgttt
      361 taatttatgt ctttaaagtt cgtcccgaga ttttacattt tctgaaaaat gaggagcagc
      421 cgaataaagt accgctgcgc ctcattttag ccagcttttc gattgtaacg attttgaccg
      481 ccggcttttt ctctcttcat gcctcaacta accccgatgg attggagtgg tcaatcgctc
      541 aaataacaga ttctacggaa ttcaacgaag aggattcact tcctgataaa agtgcggaaa
      601 ttcaacatca acattccgtt ttacctgatt acaatttcaa ggaaggtaaa actttgggga
      661 atataaacgg aacgaccgta gccggatttt tgggaagcgg gttggtattt gtcatcgccg
      721 gcgtaagcgg ttggctcatt tcaaagctga aaaagcgaaa aagagaaagt aatatcgaat
      781 aatatcggat agattttttt aaatctcttt ggatcaagat tgaggagaac ttattaatcg
      841 ttttaatgag aaaaaaagtg aaattgttcg tatcgaacag ttggcaaacg gaaacacggt
      901 tattcataaa ttacatccct atgtaaaaag tgttgttttg gttttttatt taatcactgt
      961 tctttccgta tcaccttatc aatttgatcg tctggctcct tttattcttt tccccgcagt
     1021 ggcgatctct ttggcggaaa tacccttccg atttattttt cagcggattt tgccggtatt
     1081 tccttttgta atttttgccg ggctttctaa cctttggttt gatcgaagaa ttgctttttt
     1141 tatggattcg ctgccgatta catatggcgc agtctctttt attaccctga ttctgaaaac
     1201 ttttttgacc gtgagtgccg tattgatttt attgaccacg acacctatga agtcgcttac
     1261 cgatttgttg agaagcatca aaataccgga attgattatc acccaaatca tgttgagcta
     1321 tcgttatata actttgttgg tggacgaagt atctgtcatg gaaaccgctt atttgttacg
     1381 atcttgcggg aagcagagaa tcgagttgaa acacatcggc agttttatcg gacaaatcct
     1441 tctgcgaagt tatgatcgag ccacacgagt ggcttttgcg atgaaatgtc gcggatatgg
     1501 caatatttca aatataagtt catcaaatcc tctcaaaccg gttgatattt taatcggaat
     1561 gggaatgatc ggtttgattt ttggtctgag agtatggaat atttccggtg ttttaagtcg
     1621 actctttctt taataaaagg aattaatggt taccatagaa aatttatcgg ttgaatatga
     1681 agacgggaca ttcgctctca gagcgattaa tttgaaagtt cacgacggag attcgattgc
     1741 gatcctcgga gaaaacggtg cggggaaaac aacactattt tatacaatgt tagggctttt
     1801 aaaaatttct gacggaaaga ttcttgtgaa tgatattgaa ttatcatctc cgaccctaaa
     1861 agagatacgt tctcagatcg gcttggtatt tcaaaatccg gatgatcaat tatttatgcc
     1921 gactgttgaa gaggatatcg ctttcggttt aagaaattac ggaaaaagcg aagaagaaat
     1981 caataaaaaa atccgagata tagccgaaag attgggaatt gttcatttgt taaaaaaggc
     2041 tccctataaa ttatccggtg gcgaaaaaag gttggctgcc ttggctaccg ttttggcaat
     2101 ggaaccgcag gcgattctat ttgatgaacc ctcctctttt cttgatccac gttcaagaag
     2161 aacggtaatc aatctgttaa aagaaattcg tgataccaaa atgatcgcta cgcatgatat
     2221 cgatttggct tgggaactgt gcgatcgcgc cattatttta tccaagggag aagtggttgc
     2281 cgaaggttcc attaaagaaa ttttgacgaa tgaaaaccta ttaatgaaat atgggtttga
     2341 gttgacgact tgctctcaaa ccgacagaag attttgattt gattaataag tttattccac
     2401 tttactccat tcttgtcctt tttgaatgat ttgcaatgag ttccccaaat aaagcaaagt
     2461 aaaatacagt tgaaacaatg aacttattaa ccgggtaaat gaatgtacgg aattcgagac
     2521 gcacaaaaag aatttgatcg ggccagaaac caagcgattt ggcaacaagc actcggtttt
     2581 ctgcaacgga aaaattataa tttaatcgac tatgatgaag tcagaaaacg cttagtcaag
     2641 acagtcaacg cgttacctgt acaaaaagat atcccgattg atgcgattgt gggatccgta
     2701 tctcgaaata atgatttcac aaggacgttt ttgcccaaga tggattacga taaaaaccgt
     2761 tgggtgaacg ttcggttggc gaatgaatcg atggaaggcg ttccgcccat tgatgtatat
     2821 caaatcggtg aagtttattt tgttttggat ggacatcatc gtatttcggt gatgaaaagc
     2881 tatggggcgc aatatattaa tgcgaatgtt cggatcatca acaccgaagt acccttaaaa
     2941 ccaactgatt cacccgatga aataattgta aaaacggaaa aacaagcttt tcttgatcag
     3001 accaatatcg aaaaacttat tcctgaagcc aacctggaaa tgtacctgcc cggtgaatat
     3061 cccgagttgg cggaacatat cgctgttcat cgatatttta tgggattgga ttataaaaga
     3121 gatatttcaa aggatgaagc tgtgatggat tggtatcgca cagtctatat gccggttatt
     3181 gaagttattc gttctcagaa aattatggat gaattccccg ggaaaacgga aaccgagctc
     3241 tacctctgga tcgaaaataa taaagctgct ttaaccgaag agtacggcga aggattacga
     3301 atgaccgctt tggcgtggaa attggacgaa gaatacggaa agcagaaaaa aagcatcggc
     3361 tatcgtttta agaaagcttt ctttatgatt ttttcaccgg atctttccga ttggggaatt
     3421 aagaccggag actggcggaa ggaattgcga aaggatgatt cttcgttatc catacaacgt
     3481 atgatgatat ccgtgcggga tccgattgaa gattttgatt atctgcggag tgcggtctat
     3541 ttttgtaagc aattcaatgc ctgggcggga attgtgcatg tcgtaaaaag accttctatg
     3601 ctcgattccg agtggatccg gaaatatgaa caggatattc aaactctttt ggaaaaagaa
     3661 aatgtcaagg gaaaattctt tgtgcttaag ggaaatttac tgaagaacct ttcagatcgg
     3721 gctttttgga gtgatatttc cttatttaaa atgaattaca gaccttcgcc ttcacgatac
     3781 cggtcgttgg gatcgggttg gaattctatt tttatgcgtg ttccgggacc gatatttgtt
     3841 actcccgatc tgtttccttc gaagattcaa cagattttac ttgctttttc acagagcccg
     3901 aaatctcgtg aagccatgta tttcgcatcc ggcttagcgc aaagcacggg cgggcatata
     3961 acggttgtcg tttccggcgg tgatgagcag aggcgggcag ttacccggca ggaagtagaa
     4021 aattattatc aagcgcaagg cattcaggcg gaatatgtca caagttcgga tgaacccggc
     4081 aaagcgatta ttgagaatgc gaatcgaaaa aaatccgatt tgattatcat gggcggatat
     4141 tcccgatcct atatcaaaag actttttacg ggaagtacca tcgaaaaagt cctttcggtg
     4201 acttccattc cggttattat ttgtaagtag aaattattgt tcttccaaca tttgtgctaa
     4261 tgccatcgca ccgcaagcac cggcacgatt tcctaaaata ggcggagtaa tgtactgatc
     4321 aatatgttcg agaatcatcg gatgttgtat ataaccgttc agtaaacgaa tgacctcttt
     4381 gcgaatcatc gggtaaagtt gtttctgccc cataacacct ccgcccagga caattttttg
     4441 cggggaaatc tgataaattt gggcgataca ggcttgagcc agataattag ccaaaatcgg
     4501 ccagaaagga tgatcttcgg ggaggtcttg cgggggaatc ccaaagcggg cttgaattgt
     4561 cggtcccgca catagacctt ccaaacaatt tccatggtaa gggcaatcac ccttaaaagg
     4621 atctttttca agattttgac ggatcagaat atgtccggct tcggggtgga ccaaaccatg
     4681 aaccggtttt ccgtcaataa caacgcctcc acctactccg gttccgactg tgtaataaac
     4741 gaggttgcgg atgttttccg gattgcccca tttgtattcg gcgagtgcgg cgccgtttac
     4801 atcggtatca aatccaaccg gtaaattcaa ttcttgtttc aaaaatccga cgatgtttgt
     4861 gtatctccat tttattttag gtgtcgttgt gatatagccg taagtaggtg aagaaggatt
     4921 caaatcaatg ggtccgaaac agcttactcc gatagcggta agcgcatatt tatttcgttg
     4981 ctccttgaaa aaagagaccg tgcgttcaag cgtttcttcg ggagttgtcg taggaaatcg
     5041 aacttcttca aaataatgat cgggatcagc caagatcgaa caaacaaatt ttgtaccgcc
     5101 tgcttcgatt gctccgtaat atttcataat tcacctttta tcagctaaaa tgggaagtgt
     5161 tgcaccgccg taaggcatcg cgataatcga tgcattttgt ccatattgat ttataagtcg
     5221 ttcgagggtt tcctgtggat tttgagtgga ttccaatttt atttttcgca ctagatcggg
     5281 attcagttcg gaataaagaa tgacggtcgc cttttgcatg accatcgcaa ttgcggcggc
     5341 tttatgacct cccaaaacaa attctttttg aatgtgatca atcatttcag aaggattatt
     5401 atggtgtaac atccattctt caaaagtttt ttctcccaaa ccttctttag cggaagcgat
     5461 ccacaaaatt gatccgccgt ttcgaacagc gttttttgca ttatccaacg ctttttgagc
     5521 ttgataaagg ttgatatctt tcggatatcc gcccggagaa acgaggacga tatccgcttt
     5581 ctcggggatt tcaacgctat acatttgatc caaatatcgg catccctgcc gatgagcagc
     5641 aataacgtct ccggcaacac aacggacaat tttcttatgt tcatccagta ttacatttac
     5701 aataaaatcg acggaaataa aatttaaaac cgattcgata tcggtccgaa ctggattgtt
     5761 ttcaattttt ccggcaatgg cattcggatc tgtcatatgt ctgtgatttg cctgtatcgc
     5821 ttcacgggtc gatacaccgg gcatgatcgc ttttgctccg ccgctatagc ccgcaaaata
     5881 atggtattcg atgtttccga ggcatattcg tttgtcgacc ttgacgacgc gttcaaaaat
     5941 atctaccggg gtaccattcg cggtgattcc caaacgaacc atcggtccgt cagaatcgat
     6001 acattcgtat tttttgaata tctcttcccc gacgagcatt tttttctctt cttccgtatg
     6061 agaacgatga ttcccaagcg caaaaataat gacgatatca gacgggggta ttcctgcaga
     6121 taacaactcg tctaatacaa atggcagcac acgagcagac ggcatcgggc gggtgatatc
     6181 gcttgtgata attgctattt tttcaccttt ccgaacaatt tcattcagac gtcgagattg
     6241 aatcggattt tgtaatgcct tttgcacaat ttccgattca tctccgatca tttgaatggg
     6301 atttgcctca agaaccttaa gaagattctt gtccggtata tttaattgaa cggttgactg
     6361 tccgaagcca aatgaaatat tcataagttc ctcttgtgat agattttact atcggagcgg
     6421 ttttctggtg ctgtttcgaa tgataagttc aggttgaagt aaaatatgct cttcctcgtt
     6481 atcccccgat tcgatatgac gaattaatat ttctgcggca agaaccccca tttgatttct
     6541 cggttgagca atggtggtca tttggatttg cggtaatgat gcaaagtaaa cgtcatcaaa
     6601 accgaccaca ccaaaatc
//
LOCUS       DAOMIV010000047         6604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11722_length_6604_cov_3.644679, whole genome shotgun sequence.
ACCESSION   DAOMIV010000047 DAOMIV010000000
VERSION     DAOMIV010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11722_length_6604_cov_3.644679"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKV59_02020"
     CDS             <1..516
                     /locus_tag="PKV59_02020"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQP45558.1"
                     /translation="WPNAPAFGIGTGFTYNQDTFVRLEMLGIYNNYRSNDSRILYTGV
                     LDQMQIDLFSKIKDLRTYACSVINPGIAASKVYENILSKSKELGINVLNELGFGHGIG
                     IEPVEPPYISAADDGIIAENMTLILKLAVRYLHGEILVTKDTLIVKPDGCQIVGRYQN
                     WDIPYQTAYTF"
     gene            complement(657..797)
                     /locus_tag="PKV59_02025"
     CDS             complement(657..797)
                     /locus_tag="PKV59_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45559.1"
                     /translation="MNEQNAYMALSIANRAYVLETGNITIEGPAHELLNDPRVKEAYL
                     GG"
     gene            complement(816..1364)
                     /locus_tag="PKV59_02030"
     CDS             complement(816..1364)
                     /locus_tag="PKV59_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45560.1"
                     /translation="MILEVNDLHVNYGAIKAVKGISFYVDKGEIVTLIGANGAGKTST
                     LRAITGLEKVAQGSVHFKDTDITNIAPHKIVAMGITHVPEGRRIFTNMTVMENLRLAA
                     LLNKDPEITKAAYEKIFDLFPRLKERYMQSTLTLSGGEQQMLAVARAIITGGELIVMD
                     EPSMGLAPKVVDEIFEVIQNLN"
     gene            complement(1348..2127)
                     /locus_tag="PKV59_02035"
     CDS             complement(1348..2127)
                     /locus_tag="PKV59_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45561.1"
                     /translation="MSVLKADHISIQFGGLRAVNNFSLEMNEKELFAIIGPNGAGKTT
                     VFNMLTGLYQPTEGAVYVNEKLMANCKPYQFTEAGLARTFQNIRLFGQATVLDNVKMA
                     QTFRTNYSYYNMLTRTGNYYEEEKRITQRSMELLEMFDLQDKRNYFASNLPYGEQRKL
                     EIVRALATDPKVILLDEPAAGMNPNEIDDVITLIRRVREELGKAILIIEHHMKMVMSI
                     AEYIKVLDFGETISEGVPEVVQNDPKVIEAYLGVHVNDSRG"
     gene            complement(2124..3167)
                     /locus_tag="PKV59_02040"
     CDS             complement(2124..3167)
                     /locus_tag="PKV59_02040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQP45562.1"
                     /translation="MVKTMKWLKRNSEVIIIPFIYILIAGLQRAGILNNYIIQVIMLG
                     GINIIMTESLNLVNGLTGQNSIGHAGFMAVGAYVSALFSKVIFNAGGLPPTSQTLVFL
                     LATLMGGIAAAIFGYLIGTPTLRLKGDYLAIVTLGFGEVIRSIIRITPPVGAARGMTG
                     IPKLANLFWVYLFVILTIYVCRNFMDSSYGRACMAIRDNEIAADTMGINTAKYKIIAF
                     VTSAFLAGVAGSMYAHTLRYLHPDVFGYTKSTDFLVYLYAGGVGSISGSILGAFTLTA
                     LPEVLRFMKEWRLVIYGGLLVVIILFRPVGMFGGNEFSFLKLKTGGIKSVGFSDLFKR
                     KGHKELSNKEGIQ"
     gene            complement(3169..4071)
                     /locus_tag="PKV59_02045"
     CDS             complement(3169..4071)
                     /locus_tag="PKV59_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQP45563.1"
                     /translation="MEKFIQLLVYGLQLGSIYALLAIGYTMVFGIIRMINMAHGDFLM
                     MGAYAAFFIVSLIFRDQMIGPAVALLVILASAGITGMIGVGVERVAYKPLRNRPKISS
                     MIAAIGVSLFTQNFFRAIPFIGPNPRTFPKLMKDVLFFIGPIQFSLVRIVVIILSVII
                     MAILYFFVMKTTMGMEMRAVSCDKDASSLMGIDVDRTISTTFFIGAGLAAISGAFYAN
                     SYPVLTVTMGATLGNKAFIAAVIGGIGDIRGAMLGGFIMGIIEIMISSVYSELAYGIS
                     FVFLIIILLMKPEGLLGKPMVEKV"
     gene            complement(4148..5305)
                     /locus_tag="PKV59_02050"
     CDS             complement(4148..5305)
                     /locus_tag="PKV59_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45564.1"
                     /translation="MKRILYVLLVFVMILGVGIATVSAEKEIPDVIKIGMISTISGEQ
                     ALDGQNMTDAIKLVQKELEASGGLDVDGKKVKIEFVIEDCEAKPEIAVNAAQKLIEQD
                     HVLAIIGPNNSGDTIASAEISQAAKIPQITNTGTNEAVTKVGDYIFRACFIDPFQGKV
                     VATFAYDNLKITKAAAIYDNADTYSAGLDKAFVDAFEKLGGTVVANEAFSGAEVKDFS
                     AQLINIKNAKPEAVFVPAHISNIPLILQQIRGMGIDAILLGCDSWDYAALPDLVGKDV
                     IEGAYYVTGFSPEAETAAEFVKAFTELAGYRPSFCSAMAYEAAHIVLNAIQHAKTIDG
                     PGIRDAMMETDLDLPSGHVRFDENRNPIKAGVILQVKDGVAHYVTSVLPEE"
     gene            complement(5804..>6604)
                     /locus_tag="PKV59_02055"
     CDS             complement(5804..>6604)
                     /locus_tag="PKV59_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQP45565.1"
                     /translation="VCLDAKKKGISHLICASGGNAGYAVAYCARTLGMKCTIVIPKSA
                     SNYMAEVIRREGADVIVHGDIFDDANEFALQITQEDKNAMFVHPFENPLLWEGHSTIV
                     DELKEQTDKPDAIVLSVGGGGLLNGVIQGIDRNGWSDVPVIACEVEGAPKFNLSLKAG
                     KPIYLDEIHTLANTLAGKTVSPESIAYAKNHNIISYLTGDANAARAVFEFIEDFRVIV
                     ELACGASMSVAYDEPEIIRPYHHIVVVACGGSGVNLPLLLQYKQIFNL"
BASE COUNT         1978 a         1530 c         1208 g         1888 t
ORIGIN      
        1 tggccgaatg cgccggcctt cggtatcgga accggcttca catataatca agatactttt
       61 gttcgtttag aaatgcttgg catttataac aactatcggt caaatgattc aagaattctc
      121 tataccggtg ttctggatca aatgcaaata gatttatttt ctaaaatcaa ggacttgaga
      181 acatatgcat gttccgtcat aaatcccggt atagccgcat caaaagtata tgaaaatata
      241 ttatcaaaat caaaagaatt agggataaat gtactaaacg aattgggttt tgggcatggg
      301 atcggtatag aaccggttga acccccttat atatccgctg ccgatgacgg aataatcgcg
      361 gaaaacatga cattaatttt aaaattggca gttcgctatt tgcatggtga aatactggtt
      421 acaaaagata cactgatcgt aaaaccagac ggttgtcaaa tcgtcggaag atatcagaat
      481 tgggatattc cttatcaaac agcttatacc ttttaatagg gtttcctatt cttcatactt
      541 atatcggcag gcaggaataa tgaatcgaaa tccggcctgc tttttttatt cgttaaatta
      601 aaaaagagca tttcgatttt tgaatcgaaa cgctctttaa gcaaacagca actgtattat
      661 ccgcccagat aagcttcttt cacccgagga tcgttcaata attcatgagc cggaccttca
      721 attgtaatat tgcctgtctc caaaacataa gcccgatttg caatgctgag tgccatatac
      781 gcattttgtt cattcaataa aatcgttgtt ccggattaat tcaggttttg tattacttca
      841 aaaatctcat ccactacttt gggcgccagt cccattgaag gttcatccat aacaattaac
      901 tcgccaccgg ttataatcgc ccgagcaacc gctaacattt gctgttctcc tcccgaaagg
      961 gtaagcgtag attgcatata acgctcttta agcctcggaa acagatcaaa tatcttttca
     1021 taagcggcct tggtaatttc cggatcttta tttaaaagag cagccagtct cagattttcc
     1081 atcaccgtca tattggtaaa aatgcgacgt ccttccggaa catgagtgat acccatcgct
     1141 acaattttgt ggggcgctat atttgttata tctgtatctt taaaatgaac cgatccctgt
     1201 gcaacttttt ccaatccggt tatcgcacgg agtgtcgaag tctttccggc accattcgct
     1261 ccgatcaagg taacaatctc acctttatca acataaaaag atatcccttt aaccgctttt
     1321 attgcgccgt aatttacatg taaatcgtta acctcgagaa tcatttacgt gaactcctaa
     1381 gtaagcttct atgactttag ggtcattttg tacgacttca ggcactcctt cgctgatcgt
     1441 ttctccaaaa tcaagcacct tgatatactc cgcaatcgac atgaccatct tcatatgatg
     1501 ctcgataatt aatattgctt ttcctaattc ttcgcggaca cgtcgaatta aggtaataac
     1561 atcatcgatc tcgttagggt tcattccggc tgccggctca tctaaaagga taacttttgg
     1621 atctgtcgca agtgcccgca caatttccaa ttttctttgt tctccgtatg gcaagttgct
     1681 cgcaaaataa tttcgcttat cctgaagatc aaacatctca aggagttcca tggatctttg
     1741 ggtaatccgt ttttcttcct cataatagtt tccggttcgg gtcaacatat tgtaatagga
     1801 gtaattcgtt cgaaatgttt gtgccatctt tacgttatcg agaaccgttg cctgcccaaa
     1861 tagtcgaata ttttgaaaag ttctcgccag tccggcttcg gtaaattgat aaggcttgca
     1921 attggccatc aatttttcgt tcacatagac tgctccttct gtcggttgat aaagacctgt
     1981 aagcatatta aagacagttg ttttaccggc tccgttggga cctataatgg caaataattc
     2041 tttttcattc atttccaacg aaaaattatt aacagcacga agacctccga attgaatgct
     2101 gatatgatct gctttcagaa cactcattgg ataccctcct tattactcag ctccttatgc
     2161 ccttttcgct taaataagtc ggaaaatcca acgcttttaa ttccgccggt cttaagtttt
     2221 agaaaagaaa actcattccc gccaaacata ccgaccggtc tgaacaagat aattacaact
     2281 aacagaccgc catagataac gagtcgccat tctttcataa atcgcaatac ttcgggaaga
     2341 gccgttaaag tgaatgcacc caagatggaa ccgcttatgc tgcctacgcc tcccgcgtac
     2401 agataaacca aaaaatcggt ggattttgta tagccaaata catccggatg taaatatcgt
     2461 aatgtatgag catacatact tccggcaacg ccggccaaaa aagcggatgt gacaaatgcg
     2521 atgattttat acttcgctgt attaatcccc attgtgtccg ctgcaatttc attgtctcga
     2581 attgccatac aagccctgcc gtaagatgaa tccataaaat tacggcacac atatatagtc
     2641 aaaatgacga acagataaac ccaaaacaag ttagctaatt tcggaattcc cgtcatcccc
     2701 cgagctgcac cgaccggagg agtgatacga atgatggatc gaataacttc gccgaacccc
     2761 aacgtcacga ttgccagata gtcgcccttt aatcgtaagg ttggcgttcc gataagataa
     2821 ccgaatatgg cagcagcaat tcctcccatt aatgtcgcga gcaagaaaac caatgtttgc
     2881 gatgtaggcg gtaaaccgcc ggcattaaaa atgactttgg aaaataatgc ggagacgtac
     2941 gccccgacgg ccataaaccc tgcatgaccg atagaatttt gtccggtcag tccgtttacc
     3001 aaattcaagg attcggtcat gatgatattg attccgccga gcataatcac ttgaataata
     3061 taattgttca gaattcccgc tctttgcagc ccggcaatca gaatataaat gaatggaatg
     3121 atgatcactt ctgaatttct ttttaaccat ttcattgttt tcaccattct atactttctc
     3181 aaccatcggt tttccaagca atccttcagg cttcatgaga aggataataa tcaaaaacac
     3241 aaatgagata ccgtatgcta attccgaata gacagaagaa atcataatct cgatgatgcc
     3301 cattatgaaa ccgcctaaca tagcccctcg aatatcgccg attccgccga taacagcagc
     3361 aataaaagct ttattcccca aggtggcccc catggttacc gttaaaacag ggtaactgtt
     3421 tgcataaaat gcaccgctga tagcggcaag tcccgcaccg ataaaaaatg tcgtggatat
     3481 ggtcctgtca acatcgatcc ccatcaacga agaggcatcc ttatcacaag aaaccgcccg
     3541 catttccatt cccatggtag ttttcatcac aaaaaaatag agaattgcca taatgatgac
     3601 agacagaata atgacaacaa tccttaccag actaaattga atgggaccga taaagaaaag
     3661 cacatctttc atcagtttgg gaaatgttct gggattgggt ccgataaatg gaatagcacg
     3721 gaaaaagttt tgcgtaaata acgaaacacc gattgcggca atcatactgg agattttcgg
     3781 acgatttctc aaaggtttat aagcgaccct ttcaaccccg actccgatca ttcccgtaat
     3841 accggcgctg gctaagatca ccagtaatgc aacagcggga ccgatcatct gatcacgaaa
     3901 tatgagactt acgataaaga aagctgcata cgcacccatc atcagaaaat cgccgtgagc
     3961 catattgatc atacggataa tgccgaaaac catcgtatat ccgattgcga gcaaagcata
     4021 aatacttccg agctgtaatc cataaacaag caactgaata aacttttcca tcttcctcct
     4081 tcttctaagt caaaaaaatt gtctggcaca attgaagttg tgccagacaa ttatgtcaga
     4141 agattaatta ttcctcaggt aaaacgctgg ttacgtaatg cgctacgccg tcttttactt
     4201 gcagaattac acccgcttta atcggattcc ggttttcatc aaatcttaca tgaccggaag
     4261 gaagatccaa atccgtttcc atcatcgcat cacgaattcc gggtccgtca atggtttttg
     4321 catgctgaat ggcattcagc acaatgtgag cggcttcata agccatcgca gagcaaaagc
     4381 tcggtcgata ccccgccaat tctgtaaatg cttttacaaa ttcggcggct gtctctgctt
     4441 cgggggagaa cccggttacg taataagcgc cttcgataac atccttgccg accaaatccg
     4501 gcaatgccgc ataatcccat gaatcacaac cgagcagaat cgcatcgatt cccatgccgc
     4561 gaatttgctg gaggatcaac ggaatattgc tgatatgcgc aggaacgaaa accgcttccg
     4621 gcttggcgtt tttgatattg atcaactgcg cgctgaaatc cttcacttcc gcaccgctga
     4681 aagcttcatt tgcgacaact gtcccgccta atttttcaaa agcatcgaca aatgctttat
     4741 ccaatccggc gctataggta tccgcattgt cgtaaatcgc cgcagctttt gtgattttca
     4801 aattatcata agcaaaagtc gcaaccactt tgccttggaa cggatcgata aaacaggctc
     4861 ggaaaatata atctcccact ttggttaccg cttcattcgt tcccgtattg gtaatctgcg
     4921 gaattttggc agcttggctg atttcggccg atgcaatggt atctcctgag ttattcgggc
     4981 ctataatcgc caaaacatga tcctgctcaa tgagtttctg ggcagcattc acggcaattt
     5041 ccggcttggc ttcgcagtct tcaataacaa attcgatttt gacttttttc ccgtcaacat
     5101 ccagaccacc cgaagcttca agctcctttt gaaccaactt gatcgcatcc gtcatgtttt
     5161 gaccgtccag cgcttgttcg cctgaaatcg tgctgatcat cccgattttg atcacatccg
     5221 ggatttcttt ttcagccgaa acagtcgcga tcccaacacc gaggatcata acaaaaacca
     5281 acaaaacata caaaatcctt ttcattttca ttctcctttt ttatttttta aatttattac
     5341 aataacgtaa taaattttta cagaaccaaa atacccaagt tattcttggt taaaccgaaa
     5401 gacggggcag acaaacgtct gcccctataa aactaactat tgcttgagac gaatccgaat
     5461 gatttgaacg gtagggaggc ttgtggattg cagaaaatta aataagagtt tttctgaagc
     5521 agatcccgct ttttcttcag agcacggatt attcgatagg acaacttttc gcaattcaac
     5581 cataattttc tttcagagat cctaaatgtt taagcttttt tgagacagtt tttattctac
     5641 aagaaaatcc tattttgtca agaagcaatt tttgttgaaa aaagaaaata ttttgccttt
     5701 tgtcaataaa agaatattca agaatgatta tcggaataag taataattaa ataatgtttt
     5761 ttctgataac caactttaat aaaaacacgt tcgaaattct ttttcataaa ttgaaaattt
     5821 gtttatattg aagcagcaaa ggaaggttta cacccgatcc gccacatgca acaactacaa
     5881 tatgatgata aggacggatt atttccggct catcgtatgc gactgacatg gatgccccac
     5941 atgccaattc gacaattacg cgaaaatcct caataaattc aaaaacagca cgagccgcat
     6001 ttgcatctcc ggtcaaataa gaaataatat tgtgattttt tgcatatgcg atcgattccg
     6061 gagaaaccgt cttcccggcc agcgtattgg ccaaagtatg aatctcatcc aaatagatcg
     6121 gttttccggc tttgagagat aaattaaatt taggtgcgcc ttcgacttca cacgcaatca
     6181 ccggaacatc gctccaaccg tttcggtcta ttccctgaat aacaccattc aaaagaccgc
     6241 ctccgcctac cgagagaaca atcgcatccg gtttgtcggt ttgttctttt aattcatcga
     6301 caatcgtgga atgcccttcc caaagaagtg gattttcaaa gggatggaca aacatcgcat
     6361 ttttgtcttc ctgtgtaatt tgcaaggcaa attcattcgc atcatcaaaa atatcgccat
     6421 gaacaatgac atcagcgcct tctctgcgaa taacttccgc catatagtta ctggcagatt
     6481 taggaattac gatcgtacat ttcattccca aagttctggc gcaataagcg accgcatacc
     6541 ccgcgtttcc acccgaagca cagataagat ggcttattcc ctttttcttg gcatccaagc
     6601 aaac
//
LOCUS       DAOMIV010000048         6542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_11890_length_6542_cov_3.803299, whole genome shotgun sequence.
ACCESSION   DAOMIV010000048 DAOMIV010000000
VERSION     DAOMIV010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11890_length_6542_cov_3.803299"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKV59_02060"
     CDS             <1..784
                     /locus_tag="PKV59_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45566.1"
                     /translation="QLLDKYAFLDFLFPGKYPTNNEALYDWLRSAEHLINILAREFRE
                     DESSNLISGMAVLKLGSFRDKLNEFFYNDNNLIHERRSLLIFSIIARFYRTEENSRSV
                     SEILKSRSKKMLLSTAEMKLIIKSYEAFMELHSLFNLEEYSDVVIYRYFQKFGDYGID
                     GIFLKLIETHINKDILRDSALWTKVLDHAARYFDAWFNHFDTIIKPKPLLSGDQISKL
                     FNIPAGPEIGELKNAILEAQIENLITDREGAIKFTRNLIESS"
     gene            complement(789..3221)
                     /locus_tag="PKV59_02065"
     CDS             complement(789..3221)
                     /locus_tag="PKV59_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQP45567.1"
                     /translation="MKKRNLFWEVFLVIGISVLLLGCDTKRNELIRGFFYTETPIPPT
                     PTVTITPTLSPTSIPTPLPEYRIDQADKYLLVGDYDNAISEYEKVLSSNPEEPYYAAS
                     VLGKARSYYFKRDYESCQNTLIDGLNTIKENSNKPDLWFQMADCVHELDLYEEEINAL
                     NEFLKLRPDTQMRSEIYERIGDSYYSLQSYKESQNAYLSAVEGTDMEESSWIWMKIAD
                     TYSVQEDYTNALKTWLDLYDLSTDDTQKARIDSLLADVYLKLDSPEQAYARYQDAVNN
                     FPRTYDSYLALLTLLNSDQTVNEYQRGLINYYQGQYALSSEAFHRYIESNPAHNGSAY
                     YYIGLNQMFLGDYQSAVETFATLIENYPDNQFYASAWDEKSYVEWYYLENFAQGAQTL
                     IDFVKLHPDYPEAPGFIYEAGRIFERGDRLTQAATQWERLIDEYPLYENSIDALFLSG
                     ICRYRTKNYDAALSTFNRLLLVSTQPADIARAHFWIAKVYDKKGESGSAKQNYESAAQ
                     DSPTDYYTERAKEILAGKKPLSFSENYNLTTNLSQAQVIAEQWMRLTFSIPDEVPLDQ
                     PDALLLDSDYRHADELWELGHYQDAIAMFDAVRLKYENDPVNSFRLLNRLVSLGAWRP
                     AVYTSRQILTCAGLIEDARTLEAPNYFNWIRYGTWFNEIVQEVFDQYKIHPFILYGLM
                     RQESMYDPWISSSAGASGLMQIMPATGLELSKKLQWPPNYADSDLLRAMVAINFSADY
                     LSTQHDYFGNDNFYMLASYNAGPGSTSGWVDKAENDPDLFLEIIRFEETRTYIRNVYE
                     YAKMYERFYGVG"
     gene            3330..4991
                     /locus_tag="PKV59_02070"
     CDS             3330..4991
                     /locus_tag="PKV59_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQP45568.1"
                     /translation="MTAQRKTSSVTSIIKGSLLLLIPVGFFLLFYLLPLCTIFQTVIT
                     QFLEDFHINWDKIGKVFGFTTWQAVLSMILTMILGMPIAWIFSRYIFIGKKFLKSLFS
                     VPFILPTVVTAAAFNALIGPKGLLNVYLMQKHGFSQPPIQLINTIWIILIAHIFYNIS
                     VVMRIVGNAWENIDIKLENAASTLGCTPWQTFWKITFPLLKPAIFSAMLLVFLFDFTS
                     YGVVLLLGGAKFRTIEVEIAQQALQLFNLPVAGLLSILQIIVTVAVTSIENKIGKNIQ
                     SNRMPHVSEENMRKPTKPSEKIIIILILFLVTVFLVSPLLGLVIRSFVVYDSQSVAWT
                     TEYYKKLFVNERNSFFYVPPILAVGNSLLNATIAAFISLMIGLMVTFAGDRYPWTKKI
                     NMIFLFPIGTSAVTLGLGYLLYFSHEINNPLLIPLAHSLIGLPFVIRSLQPVLQNIPI
                     SLHHAATVLGANPWKVFLKIDIPILRRAMINAGIFSFTISLGEFGATSFLSMPDRPTI
                     PIAIYRFLGQPGASNYGQAMAISTILMTFCLVGTFILESNSFQKQ"
     gene            5034..6074
                     /locus_tag="PKV59_02075"
     CDS             5034..6074
                     /locus_tag="PKV59_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45569.1"
                     /translation="MLQLKDISYQYEKTPLLENLNLHLADHEILCLLGKSGSGKSTLL
                     RIIAGLEKPDHGDIFWNGVSILNVPPFKRNFGLMFQDYALFPHLTVFENIAFGLEMNK
                     VNSEKIPPIVEKLLKQVGMESFANRKVTELSGGEQQRVALARALAPQPHLLMLDEPLG
                     ALDHTLRVSLLAELRRILGENGIPSVYVTHDQDEAFSIADRIALLHDGVIVQTDTPEN
                     IFLKPKSVWVARFLGLNNLLPAIGVTDSQILLKLNDRTIYLTIPLEYPLVEGQKIWVL
                     FRDEKLQLTNDADFSIKGIIKESIFKGSNYETHVEITPGTVFSVINEKKFQAGTEIEL
                     FYSPEDLLALLI"
BASE COUNT         1973 a         1293 c         1243 g         2033 t
ORIGIN      
        1 ccaattgctt gataaatatg cattcctcga cttccttttt ccgggtaaat atccgacaaa
       61 taatgaagcg ttatatgatt ggctacgttc tgccgaacat ttaataaata tattagctcg
      121 ggaatttaga gaagacgaat catcaaatct tatttccgga atggctgtat taaagttagg
      181 ctcctttcgt gataagctca atgagttttt ctataatgat aataatctaa ttcatgaaag
      241 aagatcactt ctgatattct cgatcattgc gagattttat cgaacggaag agaattctcg
      301 atccgtaagt gagatattaa aatcacgatc aaagaaaatg ttgctcagta cagcggaaat
      361 gaagctcatt atcaaatcgt atgaagcatt tatggaactt cattcactgt tcaatttaga
      421 ggaatattcg gatgtagtta tatatcgata tttccaaaaa ttcggcgatt acggaatcga
      481 cggtattttt ctaaaattaa ttgaaacaca tataaataaa gatatattaa gggattccgc
      541 tctatggacg aaagtgttgg accatgctgc aagatatttt gatgcttggt ttaatcactt
      601 tgatacgatc attaaaccaa agccgcttct ttcaggtgat cagataagta aactatttaa
      661 tattccggcc ggtcctgaaa tcggggagct gaaaaatgcg attttagaag ctcaaattga
      721 aaatttgata acggatcgcg aaggtgctat taaatttact cgaaatttga tcgaatcttc
      781 ctgagttatc aacctacgcc gtaaaatctt tcatacattt ttgcatattc gtaaacattt
      841 cggatatagg tacgcgtttc ttcgaaacga ataatctcta agaataaatc aggatcattc
      901 tcggctttat ctacccaacc ggaggtgctt ccggggccgg cattataaga agcaagcata
      961 tagaaattat catttccgaa atagtcgtgt tgtgtcgaaa ggtaatcggc ggaaaaatta
     1021 atcgcgacca tcgcccgtaa caaatcgcta tcggcgtaat tcggcggcca ttgtaatttt
     1081 ttagataatt caagaccggt tgccggcata atttgcatca gaccggatgc tccggctgaa
     1141 gaagaaatcc acggatcata catactttcc tgtcgcatca aaccgtagag aatgaacgga
     1201 tgaattttat attgatcaaa aacttcttgg acgatttcat taaaccatgt cccgtaacga
     1261 atccaattga agtagttcgg ggcttccaat gttcgcgcat cttcgatcag acccgcgcat
     1321 gtaagaatct gtcgacttgt gtaaaccgcc ggtcgccacg ctccgagtga aacaagcctg
     1381 tttaatagtc tgaaagagtt taccggatcg ttttcatatt tgagtcgtac cgcatcaaac
     1441 atcgcgattg catcttgata atgtcccaat tcccaaagct catcggcatg ccgataatcc
     1501 gaatccaata ataacgcatc cggttgatcc agcggcactt cgtccggaat cgaaaatgtt
     1561 aatcgcatcc attgttccgc gatcacctgg gcttgggaca gatttgtcgt aaggttgtaa
     1621 ttctccgaaa aagatagagg ttttttcccc gcaagaattt cctttgcacg ttcggtataa
     1681 taatcggtgg gtgaatcctg agcggccgat tcgtaatttt gcttcgcact cccactttct
     1741 cctttcttat cataaacttt tgcgatccaa aaatgtgccc tcgcgatgtc cgccggttgt
     1801 gtgctgacca aaagcaaacg attaaatgtg gaaagggcag cgtcatagtt ttttgtacga
     1861 taccgacaaa ttccggataa aaacaatgca tcgatactat tttcatataa gggatattca
     1921 tcgattaatc gctcccattg tgtcgcagcc tgagttaaac gatctccccg ttcaaaaatt
     1981 cttcccgctt catagatgaa tcccggtgct tcaggataat ccggatgtaa cttaacaaaa
     2041 tcgattagcg tttgagcacc ctgcgcaaaa ttctccagat aataccactc aacatatgat
     2101 ttctcatccc aagctgaagc ataaaattgg ttatcgggat agttttcaat caatgtagca
     2161 aatgtttcga ctgcgctctg ataatcaccc agaaacattt gattgagacc gatataatag
     2221 taagctgatc cattgtgagc cgggttgctt tctatataac gatgaaaagc ttcggatgaa
     2281 agggcatatt gaccttgata gtaattaatc aaaccacgct gatattcatt gacagtctga
     2341 tctgaattca ataaagtcag cagagccaaa taactgtcat atgttcgcgg aaaattattt
     2401 acggcatctt gatatcgggc atatgcttgt tccggggaat ccagtttcag gtacacatcc
     2461 gctaaaagtg aatcgattct tgctttttga gtgtcatcgg tcgaaagatc ataaaggtca
     2521 agccacgtct tcaacgcatt tgtataatct tcctgaacgg aataggtatc ggcaattttc
     2581 atccatatcc agctcgattc ttccatatcg gtgccttcaa ctgccgatag gtaagcattt
     2641 tgagattcct tataagattg gagagaataa taactgtcac cgatccgctc atatatttcg
     2701 gatcgcattt gtgtatcagg acgtaatttc agaaactcat tcaatgcatt gatttcttcc
     2761 tcatataagt ccaattcgtg aacacaatcc gccatttgaa accacaaatc aggtttgttg
     2821 gaattttctt taatcgtatt caagccatcg atcaacgtat tttggcagct ctcatagtca
     2881 cgcttgaaat aataagaacg agctttccct aaaaccgaag cggcataata aggttcctca
     2941 ggattggagg aaagaacttt ttcatattcg cttatcgcgt tatcataatc tccgactaac
     3001 agatatttat cggcttggtc aattcgatat tccggtaacg gagtggggat cgaagtggga
     3061 gataaagtcg gagtaatcgt aacggttggt gtaggaggaa tgggggtttc cgtataaaag
     3121 aacccccgaa ttaattcatt tcgctttgta tcacatccta acaataagac actgatacca
     3181 ataaccaaga aaacttccca aaacagattt ctttttttca taccccattt ataaccataa
     3241 atcgatgttt tcgtcaattc aatatgatct aaacagaatt tttctcatcc ggatgaaatg
     3301 ctataatcag agacggggtt atcaaaacag tgaccgctca aagaaaaaca tcatccgtta
     3361 cttcaattat aaaaggcagt ttgctgctcc ttattccggt cggattcttt cttttattct
     3421 atttacttcc cttatgtaca atttttcaaa cagtcattac tcaattttta gaagactttc
     3481 atattaattg ggacaaaatc ggaaaagtct tcggtttcac aacttggcaa gctgttttat
     3541 ccatgattct aaccatgata ctcggcatgc cgattgcatg gatcttttca cgttatatat
     3601 ttatcggtaa aaaatttctc aaatccttat tttccgtccc ttttatcttg cctaccgtag
     3661 taaccgctgc ggcatttaat gcattgatcg gtccgaaagg cttattgaat gtttatttga
     3721 tgcaaaaaca tggattttct caaccgccca ttcaattaat aaacacaatc tggattatat
     3781 taatcgcgca tattttttac aatatatccg ttgtaatgcg gattgtcgga aatgcctggg
     3841 aaaatatcga tatcaaattg gaaaatgcgg cgagcacctt gggatgcaca ccatggcaaa
     3901 cattttggaa aattactttt cctctattaa aaccggcaat tttctctgca atgttgctcg
     3961 tttttttgtt tgatttcacc agctacggtg tagtgctttt gttggggggg gccaaattca
     4021 ggacgattga agtcgaaata gcacaacaag ctcttcaatt attcaatctt ccggttgccg
     4081 gtctgctttc aattctgcaa attattgtta cggtcgccgt aacttctatc gagaataaaa
     4141 tcggtaagaa cattcagtcc aaccgtatgc cccatgtttc cgaagaaaat atgagaaaac
     4201 cgactaaacc atccgaaaaa atcatcatca tcttgattct ttttctggtt actgtttttc
     4261 tagtttcacc gttgctgggc ctagttattc gttcttttgt tgtctatgat tctcaatcgg
     4321 tcgcttggac aacggagtat tataaaaaac tttttgttaa tgaacgaaat tccttttttt
     4381 atgttcctcc tattttggcg gtcggaaatt ctttgttgaa tgcaaccata gctgctttta
     4441 tttctttgat gatcggtttg atggtcacct ttgctggaga tagatatcct tggacaaaga
     4501 aaataaacat gattttttta tttccgatcg gtacctccgc tgttacgctg ggattaggtt
     4561 atctgcttta tttcagccat gaaatcaata atcctctatt aattcctctg gcacacagtt
     4621 taatcggatt gccttttgtt attcgttctt tgcaacctgt tcttcaaaat attccgatct
     4681 ccctgcatca tgcggcgacc gtattgggag cgaatccttg gaaagttttt ttgaaaatcg
     4741 atatccccat cttaaggcgg gcgatgatta atgccgggat tttttctttc accatatctt
     4801 tgggagaatt cggtgccaca tcttttctat ccatgccgga ccgacctacc attccgattg
     4861 caatctatcg ttttcttggc caacccggtg cttcaaacta tggtcaagca atggcgattt
     4921 caacaattct gatgaccttt tgtttggtgg ggacttttat ccttgaaagt aattcttttc
     4981 aaaagcaatg attttccagc cgaaaagaat tgggaagtgg gatttaataa ctgatgttac
     5041 agcttaaaga tatttcgtac caatatgaaa agacgcctct cttagaaaat ctgaacctcc
     5101 atttagcgga tcatgaaatt ctatgccttt taggaaagag cggcagcgga aaaagcacgc
     5161 tcttacgaat tattgccggt ttggagaaac ccgatcacgg ggatatcttt tggaacggag
     5221 tcagcatttt aaatgttcca ccattcaaac gaaattttgg gttaatgttt caagattatg
     5281 cattatttcc tcatttgact gtttttgaaa atattgcatt cggattagag atgaataagg
     5341 ttaattccga aaagatccct cccatagttg aaaaattgtt aaagcaagtc ggaatggaaa
     5401 gttttgcaaa tcggaaggtc acagaattat ccggaggtga gcaacaaaga gtggctttag
     5461 caagagcgtt ggctcctcaa ccacatcttt taatgttaga tgaaccgttg ggggcactgg
     5521 atcatacttt gcgtgtttct ttattagccg agctccgtag aatactgggg gaaaacggaa
     5581 ttccgtcagt ttatgttact catgatcaag acgaagcttt cagtatcgcc gatcgtattg
     5641 ccttattgca tgacggtgta attgttcaaa cagatacacc cgaaaacata ttcttaaaac
     5701 caaaatcggt atgggtggca agatttttag gcttaaataa tttgttgcct gcaattggcg
     5761 tcactgactc tcaaattcta ttaaaactta atgatcgaac aatttatttg accattccgc
     5821 tggaatatcc tttggtggaa ggacaaaaaa tatgggttct gtttcgagat gaaaaacttc
     5881 aacttacaaa tgatgcggat ttttcgataa aagggatcat caaagaaagt atatttaagg
     5941 gctcgaatta cgaaacccat gttgaaataa ctcccggaac tgttttttcc gttattaatg
     6001 aaaaaaaatt ccaagcgggg acagaaatag aactttttta ttcgccggaa gatctattgg
     6061 cacttttgat ttaacaaacc aactccatgt atctgtgaga cggaatctta cgagagttaa
     6121 caaaccgact tgtacaaaac agaaaaataa cccggttaaa agaatatttt tcccttccca
     6181 aatcgaacga tactcgtttt cggtctttga gaaaatcaat atcaaaatcg aataaagaac
     6241 agacaggaag gaaagtgctg ttattgaaca cagaaagttc aaaatctttt taaagaatat
     6301 attttcaccc ttaaaaagga tataagtcac aaaaaaaccg gaaataagaa atccgaacaa
     6361 ttgcgaattc cttttttctg gttttttatc gacagcggaa tgaaggtaga aaagccgtga
     6421 aaatattgaa agaatcatta cggaacaagc tgtccccatc aaaaatccgc ttaaaaatcg
     6481 aatcatttcc tgatctttat atatcggccg caatccgaat aatggaagta aaaaggaagc
     6541 at
//
LOCUS       DAOMIV010000049         6454 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_12134_length_6454_cov_3.840600, whole genome shotgun sequence.
ACCESSION   DAOMIV010000049 DAOMIV010000000
VERSION     DAOMIV010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6454
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12134_length_6454_cov_3.840600"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /gene="rbsC"
                     /locus_tag="PKV59_02080"
     CDS             <1..894
                     /gene="rbsC"
                     /locus_tag="PKV59_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HQP45570.1"
                     /translation="LNPNFMSKQNIINLLVAGSLIGLVAIGHTYLIIAGQNDLSPGSL
                     AAFSGVLCAYLVKIAIPLGIAILITIAAAAVVGFFNAWMVNKIKLEAFIATLVSQSII
                     RGFAYILCDGKPIAISNANFIKIGKMRFLGVPFSVWVMIVSIVIFGFILSKTKFGRSV
                     YAIGGNKDAARLAGLNPQRIVTISFVMMGIFCGIGGIIFAARMNSGQPAANVNLEFDS
                     ITAVILGGVSFTGGVGNMGGTILGIILIQAFNTGLIMVNVPTFWQYVARGALLLFALT
                     SDYIRKERHEKELLEASMRNI"
     gene            953..1933
                     /locus_tag="PKV59_02085"
     CDS             953..1933
                     /locus_tag="PKV59_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQP45571.1"
                     /translation="MKKVCIFVLLLTMVFGFVMSVSAEDNKLVVYGIYKAGDQTWFID
                     EGAAAKKAVEAAGGEFIFVDAKMSPEEYLKAIDNAIANKATGIVTCIPDQTLSQAVVD
                     KLQEAKIPVVAADDALQDADGKKLVPWVGINAYVIGEANGEWLANYAKENNLVTDPEV
                     GLLILTMDTVSSCVPRAEGEYNKFTELLPEFDKSKIFKSDYDGTTEKGNIAASAIFTA
                     HPEIKKWLVTGANEEGTIGAVRALESIGLDKDACVVGLGGYLAKDEFKKEGGSAMKAA
                     SYFSSDAVGTTSVKILLDIIAGKEVPMETAVDAIMVTPENYVEVMGDAAK"
     gene            complement(1969..2844)
                     /locus_tag="PKV59_02090"
     CDS             complement(1969..2844)
                     /locus_tag="PKV59_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQP45572.1"
                     /translation="MKVYQEGVLPESDIYFHTCSSQAKELFFYLLCTGKYFCNERYFV
                     DRQAYDSFLILYVKKGSGYVQNGNSPLLPLDEGSVAFINCYIPHKYFTKVGWTIYWIH
                     FDGLLAPKYFNLITEKNLILDPQNPHIIERYLQKIFNTFHEHKRVNEAIISKNIIDLL
                     TELILVATDSESLDNPPRMIEEMLTYISENSSQKLSLSELAKQASLSPYYFSRLFKKE
                     TGYSPFEYVIQVRIDQAKFLLKTTSMPIKELAFQCGFNSVCSFCTSFKKKTGYTPTEY
                     RIKELPNGKKMPSLM"
     gene            3053..6376
                     /locus_tag="PKV59_02095"
     CDS             3053..6376
                     /locus_tag="PKV59_02095"
                     /inference="COORDINATES: protein motif:HMM:NF028438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HQP45573.1"
                     /translation="MNHIKNQVEIWEEEVSIPTYEVGIPEKNPMFFENRVYQGSSGKV
                     YPFPIIESISNKKIDKKYQAVFLENDYLFVMILPELGGRIQRALDKSNNYDFVYYNEV
                     IKPALVGLTGPWISGGIEFNWPQHHRPTTFLPVLYRLQENSDGSKTVQMSDMDRMYGT
                     KEVTNITLYPDKAYIEIKGQLYNPTSSPQTFLWWANPAVPANEYTQSIFPPDVTAVMD
                     HGKRDVSKFPIADGIYYKYDYSKGVDISYYKNIPVPTSYMAYRSDYDFVGCYDHQREA
                     GILHIADHHISPGKKQWTWGCGDFGKAWERNLTDHNGAYIELMAGVYTDNQPDFTWLK
                     PFEEKRFTQYFLPYKKVGQVKNATKDVLLSADMKGNHLEFSIYGTHLIEDIRVFISGN
                     NHTVFDQSFTVSPTQIISESVLLPKDSRQNLKLSIFDREGEVLLTYSLDRSKKNKLPS
                     PATPAPNPKDIATNEELFLHGIHLEQYRHPTFDPDPYYLEGLKRDPGDSRINNAYGML
                     ILRRGLIEESEQYFRNAIKRITMRNPNPYDGEPYYNLGYCLFLQGRFQEAFDAFYKAT
                     WSESEQEKSFYYLATIKTIQRDYDQALDFIENSLLKNLHNLKARGLKTILLRKMGRSD
                     EAKIWAEETNTIDPFDLLALNERKLLSDDTVRLPFTIRPENFNFVIEAASDYASYGCY
                     KEAIDLLDRLEPKGPMIHYSLAYYYFRMGDLAESQKQLKSASESDPSYCFPNRIEDIA
                     VLNFAITQNPDDSYAYYYLGNLYYDKKQYQKALTDWKKSTELNDRFATSWRNLAIILW
                     NKEKKPQEAIAAMEKAFSLDPNDARILFESDQLYKKANYPIHDRLENLEKHQSLLEKR
                     DDLYTEYITLLNLSGQYEKALEKISGHIFHPWEGGEGKITSQYKIAHLEMAKKALVEK
                     NSKEAERLLLEALTYPLNLGEGKLIGTKDNDIYYFLGCCYEQACDEDKAVKAWIHACK
                     GSEEISGEMYYYDQPADMILYQGLALFKLTHYERSDLKFHKLIYYGEQHMEDTVTMDY
                     FAVSLPDMQLLEEDLSLRNRAHCHYLIGLGNLGLRNWEKASDHFKETLKINNSHVGAN
                     IHLALSNTAKSNQFFEKKW"
BASE COUNT         1953 a         1276 c         1423 g         1802 t
ORIGIN      
        1 ctgaatccca attttatgag caagcagaac atcatcaatc tgctggttgc cggttcgctc
       61 atcggattgg tagcaatcgg tcatacttat ttaattattg ccggacaaaa tgatttgtcg
      121 cccggctctt tggctgcttt ttccggagtc ttatgtgcct atttggtgaa aatcgcgatt
      181 ccgttaggca tagcgattct tataacgatt gccgctgccg ccgtggtcgg ttttttcaat
      241 gcctggatgg taaataaaat caaattggaa gcgttcatcg caaccttggt cagccaatcc
      301 atcattcgtg gctttgctta tattctttgc gacgggaaac cgatagcaat cagtaacgca
      361 aactttatta agatcgggaa gatgcgcttt ttaggcgtcc cgttttccgt ctgggtaatg
      421 atcgtctcaa tcgtcatttt cggattcatc ttatcgaaaa ccaaattcgg tcgaagcgtt
      481 tatgcgatcg gcgggaacaa agatgccgca aggttagccg ggttgaatcc gcaaagaatt
      541 gtgacgatat cctttgtaat gatgggaatt ttctgcggca tcgggggcat catctttgcg
      601 gcaagaatga attccggaca gccggccgcg aatgtaaacc tggaattcga ttcgattacc
      661 gcagtgattt tgggaggagt ttcctttacc ggcggcgtcg gaaatatggg agggaccatt
      721 ctgggaatta ttctgattca agccttcaat accggcttga tcatggtaaa tgtgccgact
      781 ttctggcaat atgtcgcccg cggagctttg ctcttatttg cgctgacttc cgactatatc
      841 cggaaagaac ggcatgaaaa agagttgctg gaagccagca tgagaaatat ttgacggtca
      901 ataacaatac gaaaagaaag gtattgttaa attttttcag gaggatactg aaatgaagaa
      961 ggtttgtatt tttgttttac ttttaacaat ggtatttggc tttgtaatgt ctgtctccgc
     1021 cgaagacaat aaattagttg tctacggcat ttacaaagca ggtgatcaga cctggttcat
     1081 cgatgaaggc gcagccgcta aaaaagctgt tgaagccgca ggcggtgaat tcatttttgt
     1141 cgatgcaaag atgagccccg aagaatacct caaggcaatt gataacgcaa tcgccaataa
     1201 agccaccggc atcgttacct gcattcctga ccagacactg tcacaggctg tggtggataa
     1261 actccaggaa gcaaagatcc ccgttgttgc ggcggacgat gcgttacagg atgcggatgg
     1321 caagaaactg gtcccatggg tcggaatcaa tgcttatgtc atcggtgaag caaacggcga
     1381 atggcttgcc aattatgcaa aggaaaataa tttggtaacc gatcccgaag tcggcttatt
     1441 gatcctgacc atggatactg tttccagttg cgttcctcgt gcggaaggtg aatataacaa
     1501 attcacggaa ctgctccccg aattcgataa atccaaaatc ttcaaatccg attatgacgg
     1561 aacaacggaa aaaggaaaca tcgctgcttc cgctatattt accgctcatc cggaaattaa
     1621 aaaatggctt gtcaccggcg ccaacgaaga aggtacgatc ggtgccgtcc gcgctttgga
     1681 gagcatcgga ttagataaag atgcctgcgt cgtcggtctc ggcggatatt tggcaaaaga
     1741 tgaattcaaa aaagaaggcg gttccgctat gaaggctgcc tcttacttct catccgatgc
     1801 ggtcggtacg acttccgtaa agattttgct ggatatcatc gccggcaaag aagtccccat
     1861 ggaaacagcg gttgatgcca taatggtcac ccctgagaat tacgttgaag ttatgggcga
     1921 tgccgcaaaa taataatcag ttgtttgatt aatggaaaag atgccttcct acatcaagga
     1981 aggcatcttt tttccgttcg gcaattcttt tatccgatac tcggtcggtg tatatccagt
     2041 tttcttctta aaagaggtgc aaaaactgca gacactattg aatccgcatt ggaaagccag
     2101 ctctttaata ggcatggaag tggttttcag cagaaatttt gcttgatcaa ttcggacttg
     2161 aatgacatat tcaaaaggag aatatcccgt ttctttctta aaaagccggc tgaagtaata
     2221 cgggctcaaa gaagcttgtt tcgccaattc ggataaggaa agcttttgac ttgaattttc
     2281 tgaaatataa gtcaacattt cctcgatcat acggggagga ttgtccaaag attcgctatc
     2341 ggtagctacc agaatcaatt cggtcaacag gtcaatgata ttttttgaaa taatcgcttc
     2401 gttgactctt ttatgttcat gaaaggtatt aaatattttt tgcaggtagc gttcgatgat
     2461 atgcggattt tgcggatcaa gaatcaaatt cttctcggtg atcaagttaa aatacttcgg
     2521 cgccaataat ccatcaaaat gaatccaata aattgtccat ccgaccttgg taaagtattt
     2581 atgagggata taacaattta taaaggctac agatccttca tcaaggggta acaagggtga
     2641 attcccgttc tgaacatatc cggaaccttt tttgacatat aaaatcagaa agctgtcata
     2701 agcttgtctg tcaacaaaat agcgttcatt acaaaaatat ttgccggtac agagtaaata
     2761 aaagaataat tctttcgctt gcgaactgca ggtatggaaa taaatatccg attccggtaa
     2821 taccccttcc tgataaactt tcatcttttc tgcctttcca aaaacagcaa ttttttttac
     2881 tcgctctttt gaagtattct aaaatatttc ggaaaatcgg tcaaattgac caaatttttc
     2941 attttaacaa aggcaatata tttaaccaga atagcaagat attatcttgt ttgaactaaa
     3001 ctgcatgcta agatatattc agatagttta ttttttcaaa ggataggcaa tcatgaatca
     3061 tatcaagaat caagtagaaa tttgggaaga ggaagtttca ataccaactt atgaagttgg
     3121 cattccggaa aaaaatccca tgttttttga aaatcgggta tatcaaggga gcagcggaaa
     3181 agtctatccg ttcccgatta tcgaaagtat ttcaaataaa aaaatcgata agaaatatca
     3241 agcggttttc cttgaaaatg actatctgtt tgtcatgatt ctccccgaat tgggaggaag
     3301 aatccaacga gcgctcgata aatccaataa ttatgatttt gtttactata acgaagtgat
     3361 taaaccggcg ttggtcggac ttaccggacc ttggatcagc ggcggaatcg aattcaattg
     3421 gcctcaacat caccggccga ccactttttt acctgtttta tatcggctgc aagagaattc
     3481 ggacggaagt aaaacagtcc aaatgagcga tatggaccgt atgtacggca caaaagaagt
     3541 gacgaatatt acgctgtatc cggataaagc atatattgaa ataaaaggac agttgtataa
     3601 tccgacctct tcgccgcaga cttttctgtg gtgggcaaat ccggctgttc cggcaaatga
     3661 atatactcaa tctatttttc cgccggatgt taccgctgtg atggatcacg gtaaacgaga
     3721 cgtttcaaaa ttccccatag ccgacggaat ttactataaa tatgactata gcaaaggggt
     3781 tgatatttct tattacaaga atattccggt cccgacctct tatatggctt atcgatcgga
     3841 ttacgatttt gtaggttgct atgatcatca gcgggaagct gggatattac acatcgccga
     3901 tcatcatatt tctccgggga aaaaacaatg gacatggggc tgcggtgatt tcggaaaagc
     3961 ttgggaaaga aatttgaccg atcataacgg cgcttatatt gagttgatgg caggtgtata
     4021 tacggataat caacccgatt tcacctggct gaaacccttt gaagagaaac ggttcacgca
     4081 atattttctc ccctataaaa aagtcggaca agtcaaaaat gcgacgaaag atgttctgtt
     4141 gtcagcggat atgaaaggaa atcatttaga attctcgatc tatggaactc atttgataga
     4201 agatattcgc gtttttattt ccggaaataa tcacaccgtt tttgatcaat cgtttacggt
     4261 ttctcctacc caaatcataa gcgaatccgt tctcttaccg aaagacagcc ggcaaaatct
     4321 caaattgtca atcttcgatc gggaagggga agttttattg acctattctc tcgatcggtc
     4381 aaaaaagaat aagctgccgt cgcccgcgac tcctgcaccg aatccgaaag atattgcgac
     4441 aaatgaggaa ttattcctgc atgggattca tttggaacaa taccgccatc caacctttga
     4501 tccggatccg tattatttgg aggggttaaa aagagatcca ggggacagtc gcatcaataa
     4561 tgcctatggg atgttgattc tgcgtcgcgg attaatcgaa gaaagcgaac aatattttcg
     4621 gaatgcgatt aaacggatca cgatgcgtaa tcctaaccca tatgacgggg aaccgtatta
     4681 taacctcggt tattgtcttt ttctccaagg ccgtttccaa gaagcctttg atgctttcta
     4741 caaagccact tggtcggaaa gtgagcagga aaaatcattt tactatcttg caaccatcaa
     4801 gacgattcag agagattacg atcaagcatt ggattttatc gagaattccc tcttgaaaaa
     4861 tcttcataat ctgaaagcac gcggtttaaa aacgattctg ctcagaaaaa tggggcgcag
     4921 cgatgaagca aagatctggg ctgaggaaac aaataccatc gaccccttcg atctgttagc
     4981 cttgaatgaa cggaaacttt tgagcgacga cacagttcga cttcctttca cgatccgtcc
     5041 ggagaatttc aattttgtca ttgaagcggc atcggactat gcatcctacg gatgttataa
     5101 agaggcgatc gatttgttgg atcgcctcga accgaaaggt ccgatgatcc attattcgct
     5161 ggcttattat tatttccgga tgggagatct tgccgaatca cagaaacagt taaaatccgc
     5221 ctcggaaagc gacccgagct attgttttcc gaaccgaatt gaagatatcg ctgttttgaa
     5281 ttttgcgatt acacaaaatc cggatgacag ttatgcctat tattatctcg gaaatttgta
     5341 ttacgataaa aaacaatatc aaaaagcttt aaccgattgg aaaaagtcga cggaattgaa
     5401 tgatcgcttc gcgacttcat ggcggaatct ggcaattatt ctttggaata aagagaaaaa
     5461 gccgcaggaa gccatcgctg ccatggaaaa agcattttct ctcgatccga atgatgccag
     5521 aatcctgttc gaatcggatc agctgtacaa aaaagcgaat tatccgattc acgatcggct
     5581 ggaaaacctc gaaaaacatc aatcgctgct tgagaagcgg gatgaccttt ataccgaata
     5641 tattacactg ttaaatcttt cgggacaata tgaaaaagca ttagaaaaga tatccggcca
     5701 tatctttcac ccctgggaag gcggtgaagg gaaaattact tctcaatata aaatcgctca
     5761 tttggaaatg gcaaaaaaag ccttggtaga aaaaaattcg aaagaagcgg agcgactttt
     5821 actggaggct ttaacttatc ctctgaattt aggagaggga aaattaatcg gcacaaagga
     5881 taacgatatc tattatttct tgggatgctg ttacgaacaa gcttgtgacg aagacaaagc
     5941 cgtgaaagca tggatacatg catgtaaagg ttccgaagag atttccggag agatgtatta
     6001 ttatgaccaa ccggcagata tgatccttta tcagggtttg gcacttttca aattaacgca
     6061 ttatgaaaga agtgacctaa aatttcacaa gttaatttat tacggtgaac aacacatgga
     6121 agataccgta acgatggatt atttcgcggt ttcgttaccg gatatgcaat tgttggagga
     6181 agatctgtcg ctgcggaatc gtgcccattg tcattatctg atcggtctgg gaaatttagg
     6241 attaaggaat tgggagaaag cctcggatca tttcaaggaa actctaaaga taaataacag
     6301 tcacgtcgga gcgaacatcc atctggcatt atcgaacaca gcgaagtcaa accaattttt
     6361 tgaaaaaaag tggtaagaaa cgggagaggg aatttttgtc ggggatggcg tccttgacat
     6421 cccgcagaat atgataccaa tcttgtcgca ttat
//
LOCUS       DAOMIV010000050         6421 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_12213_length_6421_cov_4.007069, whole genome shotgun sequence.
ACCESSION   DAOMIV010000050 DAOMIV010000000
VERSION     DAOMIV010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12213_length_6421_cov_4.007069"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PKV59_02100"
     CDS             <1..851
                     /locus_tag="PKV59_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45574.1"
                     /translation="VEKPWNAFGIGLVTVIAAPFVFILFCMTVILIPLAFLLILALIL
                     LIFYGWIACGYEAGQWMTKSMKVTWTSNWTTAFGTFVITLVAHGLKKIIPCVGWMPGG
                     IAALFGLGGVMIYYYHRYQDRPKKSTSNPQLPKNNPPSGDYRPRENIIPQSSSEEEKM
                     RQSREEPVAEKSEKGPNDSQKPESVIELPGKVETGETEEGSAQNGIEEMRGAEPVSDF
                     DASEPQKETGESSASPETPPKKKKRGVGASVLDSIHESEMKDHATSEDHLQTSNETDK
                     GDSKES"
     gene            1476..1751
                     /locus_tag="PKV59_02105"
     CDS             1476..1751
                     /locus_tag="PKV59_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45575.1"
                     /translation="MPANGEERKIELIGTSGTPSPTVESKKIPADRDVEDAVPYRISE
                     KISANRNVGDAVPYGKTEKISANRDVGDAVPYGKIEKILADRDVSTS"
     gene            complement(1845..2453)
                     /gene="nadD"
                     /locus_tag="PKV59_02110"
     CDS             complement(1845..2453)
                     /gene="nadD"
                     /locus_tag="PKV59_02110"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQP45576.1"
                     /translation="MTQDRLGIFGGTFDPPHLGHMILAAEAADQLNLDHVLWMLSPTP
                     PHKRQNIISDFEERAELVSACIEGESRFELSTLENERPGPHYTADTLKILKERFPDSK
                     IVFLIGGDSLQYLPTWHEPQLVMERIDELGVMQRPGEVFDWKELKNILPDIDSKLRMI
                     DAPLLEISSTEIRERVKSGHHYKYYLRDSVYRLILQKKYYVN"
     gene            complement(2450..3715)
                     /gene="obgE"
                     /locus_tag="PKV59_02115"
     CDS             complement(2450..3715)
                     /gene="obgE"
                     /locus_tag="PKV59_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQP45577.1"
                     /translation="MFVDEAEIYVRSGKGGDGMMHFHREKYVSRGGPDGGDGGRGGDL
                     ILKVDRRLNTLAQFRHISRFIAEDGQNGGPNNMTGRSAEDKIVLVPAGTIVRDAVTND
                     LIGDLVNEGQELIVARGGRGGRGNTHFVTPSYQAPRVAEKGAPAEERRLKLELKLIAD
                     VGIVGVPNAGKSSFLAAVTNARPKIADYPFTTVEPNLGVAELDLDHSLVLSDIPGLIE
                     GAHMGVGLGDSFLRHIQRTKVILHLLDGLAEDPIADFDQTNQEMALFDEKLIEKPQLV
                     AFNKMDIPEAAERWPEVEKFLKEKGYQAMPISAATGQNVQAVLWKLYEMLQNVPEELP
                     TESLPVYLPKEDPRKFEITKDAEGDWVVRGVSIERAAKMTFWEHSGSVRRFQTLLRTL
                     GIEDALREAGVQNGDTVMIGDDFEMEWIE"
     gene            3947..4186
                     /locus_tag="PKV59_02120"
     CDS             3947..4186
                     /locus_tag="PKV59_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQP45578.1"
                     /translation="MSENYSDYEKLKGLIEELDDYINHYHGGSVNLISFDGKVVRVKL
                     GGACEGCPLSPSTLHGWVEGTIRQFFPEIESVESV"
     gene            4283..4621
                     /locus_tag="PKV59_02125"
     CDS             4283..4621
                     /locus_tag="PKV59_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45579.1"
                     /translation="MRRGISIFFGMVLIIVGLLKLLPLVGVNVIIDGFNLDTWFPVVY
                     ILIGLLELMNQEASGWFWGIVFLVYGVFKLSQIIGFAIPLINMLNIESLLIPFFVLIY
                     GIKAIATSGE"
     gene            4648..5325
                     /locus_tag="PKV59_02130"
     CDS             4648..5325
                     /locus_tag="PKV59_02130"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF009299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQP45580.1"
                     /translation="MEFLLDTIDISEIEKCLAIFPISGITSNPSIIKQYGKVDFFSHF
                     KDIRSLIGAERSLHIQMVSEDADGILAEAYSVLEKIDHDVFIKIPVTEQGLKAIQILK
                     KDDAMVTATAIYTEAQALLATAAGADYLAVYYNRMENMDIDPCHVISATAKLIERNQL
                     KTYLLGASFKNMGQVQKAFEAGANAVTVQPSLLYDALKMPAIQKAVDDFRADWAALYG
                     NVSIAAL"
     gene            complement(5322..6155)
                     /locus_tag="PKV59_02135"
     CDS             complement(5322..6155)
                     /locus_tag="PKV59_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQP45581.1"
                     /translation="MASVKMLSYIKRDSPIHRLCGVTKLYTFLVWTIVCMLSYDTRVL
                     LVVMALSIIIFYLAKIKLEEVRFVLLFILFFLLLNNITIYLFSPEEGVKIYGTRHVLF
                     QFTNRYTVTKEQLFYLFNVTLKYFTVVPIALLFIVTTDPSEFASSLSRIGISYRISYA
                     VSITLRYIADIQSDYHNISQSQQARGVDLSKNVPLRKRIAGISSIAVPLIFSSLDRIE
                     LISNAMDLRGFGKKEKRTWYCARPFQRIDTIVITGVTLFGLAALIVTFHDGTRFYNPF
                     I"
     gene            complement(6143..>6421)
                     /locus_tag="PKV59_02140"
     CDS             complement(6143..>6421)
                     /locus_tag="PKV59_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45582.1"
                     /translation="TLQERGLTILLITHDMHLMLEYTRRTIVIADGRLICDDSPARVL
                     SDLNIIRPADLKETSLFTLAEKACLDDPLNFVQCFIDYDRRVRKTWHQ"
BASE COUNT         1816 a         1360 c         1550 g         1695 t
ORIGIN      
        1 ttgtcgagaa accatggaat gcctttggga tcggattggt gacggtgatc gcagctccgt
       61 ttgtatttat tttattctgt atgactgtga ttctgattcc actggcgttt ttgctaatct
      121 tggcgttaat ccttttgatt ttttatggct ggatcgcttg cggttatgaa gccggacaat
      181 ggatgacaaa gtctatgaag gtgacttgga cttctaactg gacgacagcc ttcggaactt
      241 ttgtcataac tttggtcgcc catggattga agaaaatcat cccatgtgtc ggttggatgc
      301 cgggcggaat cgcagctttg ttcggtctgg gcggagtgat gatttactac tatcatcgat
      361 accaagatcg accgaaaaag agtacttcga atccgcagtt gcccaaaaac aaccctccga
      421 gcggagatta ccgacccagg gaaaatataa ttcctcagtc gtcatccgaa gaagaaaaaa
      481 tgagacaaag cagagaagag ccggttgccg agaaatccga gaaggggcca aacgattcac
      541 aaaaaccgga atccgtaatt gaattgcccg ggaaagtcga aaccggcgag acggaagaag
      601 gctcggctca aaatggtatc gaagagatgc gtggggcgga acccgtatcg gatttcgatg
      661 caagcgaacc gcagaaagaa acgggtgaaa gcagcgcatc tccagaaact ccgccgaaga
      721 aaaagaagag aggtgtggga gcttccgtgt tggattcgat tcatgagtcc gagatgaagg
      781 atcatgcgac ttcggaagat catctgcaaa cttcgaatga aaccgataaa ggcgattcga
      841 aagaaagctg atgtaagaat tcgtttaaaa gaattttgat aagaagacgg tcggcttgcc
      901 ggccgtcttt gtttataaga gttttggttg tcgggggaaa cgtttattgc cgcgccgaag
      961 atgaagaaga tgttatttat agagagaaaa attgttatgc tgcgtaaaaa agcttttgtg
     1021 gaaagcgaaa ccatcgccct ttgggagaga tagcagtaaa aaaaacgaaa tccttccgaa
     1081 agtgttgaaa ccctcgcctt ggtaggatgc cgtcgtgaaa accctcgccc tgtgggagag
     1141 ggtagggtga gggtggtaaa ttccagtaga aaaagagaaa atttagaagg attgatttgt
     1201 cgaaaggacg tcggattttt tgcaagaccc tatgtacgtt atcgaggttt ggatgacaaa
     1261 agccgatgcc ggcaaatggt gaagagtggt agattgagcc gatcgggacg tcggggacgc
     1321 tgtcgtgaaa accctcgccc tgtgggagag gtgagggtga gggtggtaaa ttccagtaga
     1381 aaaagagaaa atttagaagg attgtcttgt cgaaaggatg tcggtatttt tgcaagactc
     1441 catgtacgtt atcgaggttt ggatgacaaa agccaatgcc ggcaaatggt gaagagagga
     1501 agattgagct gatcgggacg tcggggacgc cgtcccctac ggtagaatcg aaaaaaatac
     1561 cggcagatcg ggacgtcgag gacgccgtcc cctacagaat atccgaaaaa atatcagcga
     1621 atcgaaacgt cggggacgcc gtcccctacg gtaaaaccga aaaaatatca gcgaatcggg
     1681 acgtcgggga cgccgtcccc tacggtaaaa tcgaaaaaat attggcagat cgggacgtca
     1741 gcacctcgta accttcggtg tgcttcgcaa ggacgccgtc ccctacggta aaatcgaaaa
     1801 aaggacaccg tccccacggt aaaaccgaaa aaatgtcggg cggatcaatt cacatagtat
     1861 ttcttctgaa gaattagccg gtaaacggaa tctctcagat aatatttata gtgatgcccg
     1921 cttttaaccc gttcccgaat ttcggtggat gagatttcaa gcagcggagc atcgatcata
     1981 cgcaatttgc tatcgatatc cggtaagata tttttcaact ctttccaatc aaatacttcc
     2041 cccggtcgtt gcatgacacc caattcatcg atccgttcca ttaccaattg cggttcatgc
     2101 caagtgggca aatattgcaa tgaatctcca ccgattaaaa aaacgatttt tgaatccggg
     2161 aaacgttctt ttaaaatttt caacgtatcg gctgtgtagt gcggtccggg gcgttcgttt
     2221 tcgagtgtgg aaagttcaaa tctcgattct ccttctatgc aggcggagac cagttcggcc
     2281 cgctcttcaa aatcggagat gatattctgt cgcttatggg gcggtgtcgg tgacaacatc
     2341 caaagcacat gatcaagatt cagttggtct gccgcttcgg ctgccaagat catatggccc
     2401 aaatgagggg gatcaaaggt tccaccaaat attcccagac gatcctgtgt cattctatcc
     2461 attccatttc aaaatcatcg ccgatcataa cagtgtcgcc gttttgcaca cccgcttccc
     2521 gaagtgcatc ttcgatgccg agtgttcgta aaagtgtctg aaagcgtctt acagaaccgc
     2581 tgtgttccca aaatgtcatt ttggctgccc gttcaatcga aacaccccga actacccaat
     2641 cgccttccgc gtctttggtg atttcaaact tacgcggatc ttctttcggt agataaaccg
     2701 gtaagctttc cgttggaagt tcttccggga cattttgcag catctcataa agtttccaaa
     2761 ggacggcctg gacgttctgt cctgttgccg cggaaatcgg catggcttga tatccttttt
     2821 ctttgaggaa tttttctact tccggccaac gttcggccgc ttccgggatg tccattttat
     2881 tgaaagcaac caattgcggc ttttcgatca gtttttcatc aaacaaagcc atttcctgat
     2941 tcgtctgatc aaaatccgcg atcggatctt cggccaggcc atccagtaaa tggagaatca
     3001 ctttcgtgcg ctggatatgt cgcaggaatg aatcgcccaa ccccacaccc atatgtgcgc
     3061 cttcgattaa tcccggaata tctgataata ccaaagagtg atccaaatcg agttccgcaa
     3121 cccctaagtt cggttcgacc gtcgtaaacg gataatcggc aatcttaggc cgggcattgg
     3181 ttaccgctgc cagaaaactg gatttaccgg cattgggaac acccacaatt ccgacatcgg
     3241 caattaattt caattccagt tttaatcttc tttcctctgc gggtgcgcct ttttcggcca
     3301 cacgcggggc ttgataggaa ggggtgacaa agtgagtatt tccgcgtccg ccgcgcccgc
     3361 cgcgggcaac gatcaattcc tgtccttcat taaccaaatc gccgatgaga tcattggtaa
     3421 cagcatcacg aacgattgtc ccggcgggaa ccaagacaat tttgtcttcg gcggatcgcc
     3481 cggtcatatt attgggtccc ccattttgac cgtcttcggc gataaaacgg gaaatgtgac
     3541 gaaattgtgc caaggtattt aaacgccgat caactttcag gatcaggtcg ccgccccttc
     3601 cgccatcccc cccatcggga cctccgcggg aaacatattt ttcccgatga aaatgcatca
     3661 ttccgtcacc gccctttccg gagcggacat aaatttctgc ttcgtcaaca aacatatatt
     3721 ctctaactcg ctttctgaca acctctcttt attttacgct ataaatcctt cgattggatt
     3781 ctgcgatata gaagcaggga atggcaaatt tttatccgca tttgtttctc gggaagtgaa
     3841 aaaaattgaa ttattttgaa ttcggaagat tctcttttcg aaagcatcgg atcaatggaa
     3901 caaggtaaaa ttaacaataa taggagagcg ggaagaggag tgagatgtga gtgagaatta
     3961 ttcggattat gaaaaactga aaggtttgat tgaagaattg gatgattata tcaatcatta
     4021 ccatggcggg tcggttaatt taatttcgtt cgacggaaaa gttgtcagag tgaaattagg
     4081 gggcgcatgt gaaggatgcc cgttatctcc ttcgacatta cacggttggg ttgaaggaac
     4141 catccggcaa tttttccctg agatcgaatc tgtcgaatct gtttaaggat tgattttttc
     4201 ggcacaaaaa ttgcatcaag atcaataagt ttaggaaggg atcatattct ccggttcgaa
     4261 tcgggaagtt aggagtgtta ttatgcgtag gggaatttcc atttttttcg ggatggtttt
     4321 aatcattgtc ggtttgctta aattattgcc gttggtaggg gtaaatgtca tcatcgacgg
     4381 ttttaatttg gatacttggt tcccggtcgt atatatcctg atcggattac ttgaattaat
     4441 gaatcaagag gcaagcggtt ggttctgggg gattgttttc ctggtttatg gcgtgtttaa
     4501 actcagtcaa atcataggct ttgctattcc cttgatcaat atgttgaata tcgaatctct
     4561 gttaattccg tttttcgtac tgatttacgg tatcaaagca atagcgacat ccggagaata
     4621 aagggagacg gttagcaaag gaggtaaatg gagtttttac tggacacgat cgatatttct
     4681 gaaattgaga aatgcctggc aatttttccg atcagcggta ttacgagcaa tccttccatt
     4741 attaaacaat atgggaaagt agattttttc agtcatttca aggacattcg gtctttaatc
     4801 ggagcggaac gatctcttca tattcaaatg gtttcggaag atgcggatgg gatattggcc
     4861 gaggcatatt cggttttgga aaaaatcgat catgatgttt tcattaaaat tccggttacg
     4921 gaacaagggc tgaaagcgat tcaaatcctg aaaaaagatg atgcaatggt caccgctacc
     4981 gccatttata cggaagcaca agcgctgtta gcgaccgcag cgggggcgga ttatctggcg
     5041 gtttattaca accggatgga aaacatggat atcgatccct gccatgtcat atccgctacg
     5101 gcaaagttaa ttgagcggaa tcaacttaaa acctatcttc tcggagccag ttttaaaaat
     5161 atgggacaag tccaaaaagc gtttgaagcc ggggcaaatg ccgttacggt gcagccttcc
     5221 cttctttatg atgcattaaa aatgccggcg atacaaaaag ccgtcgatga tttcagagcc
     5281 gattgggcag cgctatatgg gaatgtctcc atcgctgctc tctaaataaa aggattataa
     5341 aagcgtgttc cgtcatgaaa cgtgacgatt aaagccgcta atccgaataa agttaccccg
     5401 gtaataacga tagtatcaat tctttgaaac gggcgggcgc aataccaggt ccgtttttct
     5461 tttttcccga atccccgcag atccattgca ttgctgatta attcgattcg atccagactc
     5521 gaaaaaatca acggtacggc aatgctcgaa attccggcta ttcgtttccg gagtgggaca
     5581 tttttggaca agtcgacgcc gcgtgcctgt tgggattggg aaatgttatg ataatcactt
     5641 tggatgtccg caatgtatcg cagtgtgatg gaaaccgcat aggagatgcg gtaagaaata
     5701 ccgattcgtg aaagcgatga ggcgaattcg ctcggatcag ttgtgacgat aaacaacagg
     5761 gcaatcggga cgaccgtgaa atactttaac gttacattga acaggtaaaa aagctgttct
     5821 ttggtaaccg tatagcggtt tgtaaattgg aaaagaacat gacgggttcc gtaaatcttg
     5881 actccctctt ccggagagaa aaggtaaatc gtaatattgt tcagcaacag gaagaataaa
     5941 ataaacaaca aaacgaaacg gacttcctcc aacttgattt ttgccaaata aaaaataata
     6001 atacttaaag ccatgaccac aagcaacaca cgcgtatcat agctgagcat acaaacgatt
     6061 gtccaaacca aaaatgtata taattttgtc acgccgcaca ggcgatgaat cggagaatcc
     6121 cgtttaatat acgaaagcat ttttactgat gccatgtttt ccggaccctc ctatcataat
     6181 cgatgaaaca ttggacaaag ttcagcgggt catctaagca ggctttttca gctaaggtaa
     6241 ataaactggt ttccttcaga tccgccggtc ggataatatt gagatccgac aggacacgag
     6301 cggggctgtc atcgcaaata agacgaccgt cggcaataac gatggtacgt cgggtatatt
     6361 ccagcatcag atgcatatca tgagtaatca aaagaattgt caatccccgc tcttgaagag
     6421 t
//
LOCUS       DAOMIV010000051         6386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_12297_length_6386_cov_4.172643, whole genome shotgun sequence.
ACCESSION   DAOMIV010000051 DAOMIV010000000
VERSION     DAOMIV010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12297_length_6386_cov_4.172643"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="PKV59_02145"
     CDS             <1..529
                     /locus_tag="PKV59_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45583.1"
                     /translation="ILNNVTSLSTTFVQKSIRQMIRDGRLSIMKDRLHFANEAALENF
                     LSHIQNFNNQILDYAMNNRKLIRILMTESLKESKHKNDLIQFIKELGKAAENPIIRTI
                     REANADFPLSNNFIFLRFFFHPVSIINFAVYYETYQKICGLSESKMRTAMLNSIRTLS
                     NSFVSGNDIILQTEA"
     gene            619..1998
                     /locus_tag="PKV59_02150"
     CDS             619..1998
                     /locus_tag="PKV59_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQP45584.1"
                     /translation="MKNIQDSIEELIIHYVVTDPLGKLPKMLNLAEKIDQGHLHENEI
                     NGIRKILDDKNGNWYKFIEGISEDIDPKCLQKVIECFMINASLKGEAKARAVEKKYDM
                     NVPWTILVDPTSACNLHCTGCWAAEYGNKNNLSYETLDSLVNQGVDLGIYFYIFSGGE
                     PLIRKDDIIRLCKAHQDCYFFSFTNGTLVDDQFCKQMLEVGNFAVAFSIEGNEEATDM
                     RRGKGTYRKVIAAMDLMKKNKLLFGYSTCYHRYNTESVGSDEFVDDMIARGCKFAWNF
                     TYIPVGKDAVPDLLATPEQRKYMYHRVREIRATKPLFAMDFWNDGDSTNGCIAGGRRY
                     LHINAAGDVEPCAFIHYSNANIHDMSLLDALKSPIFAEYRKGQPFNENHLRPCPLLDN
                     PQALARMVKASGAKSTDMEAPEDVDVLTAKTKAAAEKWAPVADELWEKQNKAKAEKQA
                     EAQKVEIEA"
     gene            2193..2990
                     /locus_tag="PKV59_02155"
     CDS             2193..2990
                     /locus_tag="PKV59_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQP45585.1"
                     /translation="MTGLKSGGKFNQTVKLKGGRMVTRVIESEDSKDRIIKAAVQLFS
                     EKGFDGTRVSDIADLADVNKALIYYYFTGKEDILDILLRQLLNDITAFSMDFIHTHLI
                     SMIRDGTLELKEDRLTFIDKNAVDSFLDSMRGYYRKAVSYALENRQIIRILMLESLKN
                     SKHQNDLYLFLKLLNKNDNNPVYKTILNADWNFNYSDEFILYKFFFSMMPIINFAVYF
                     DDYKNHSDMEEEDLINRFLQSCQTTINALVCGNEILMRNDCSIIESR"
     gene            3097..4467
                     /locus_tag="PKV59_02160"
     CDS             3097..4467
                     /locus_tag="PKV59_02160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQP45586.1"
                     /translation="MNSKVQNAMLSLLMGYLENDPMNKIPTILDLAEKMDTGHVHANS
                     FKVVRRELTDKNGVWYQFTENLFNEVDPRVIKKAVTSFFFNGILNNGTKQDKLREKYQ
                     CNIPWAILMDPTSACNLHCTGCWAAEYGNRNNLSFETLDSICKQGKELGIYFYLLSGG
                     EPTVRKEDVIKLCEANPDCFFFAFTNGTLVDDKFCDEMLRVGNITLGFSIEGDEAATD
                     MRRGKGTYQKVIAAMDRMKKHKLLFGYSTCYHRYNTESVGSDEFVDDMIAHGCRFAWN
                     FTYIPVGKDAVTDLLATPEQREYMYRRIREIRATKPIFALDFWNDGEYVGGCIAGGRR
                     YLHINAAGDVEPCAFIHYSNVNIHDVTLLEALQSPLFMAYRQGQPFNENLIRPCPLLD
                     NPQALSKMVKVSNAKSTDMEAPEDVDVLTSKTKPAAEKWAPVADELWKKHLKEKEEKE
                     KEKIAG"
     gene            4628..5995
                     /locus_tag="PKV59_02165"
     CDS             4628..5995
                     /locus_tag="PKV59_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HQP45587.1"
                     /translation="MDEIKTIVDMQRRFFLSGKTLPVSQRKKALQSLLTMVDENQQMI
                     FDALFTDLHKSEFESYMTEVGIVLTEIRQAMKNLDAWTSVKKVRTPLYLFPSKSSVYP
                     EPYGTVLIMAPWNYPFQLCLVPLIGALAAGNTAVLKPSAYAPAVSRCIAELIHRYFPS
                     EYIAVTEGGREENSSLLEQHFDLIFFTGGSRMGKIVMESAAKNLTPVILELGGKSPCI
                     VDQTADLKNASRKIVFGKLLNAGQTCVAPDYLLVHESVKDTLVRNLIEEFDKAFPGKD
                     YSDFPAIINEKHFDRLNRLIRDENVIYGGNSNRDRRFFEPTILFPVQWETPVMQEEIF
                     GPILPVLTYENLDDVIDRLRKLPKPLALYLFTEDRKTEKKIIENVAFGGGCMNDTVMH
                     LSNPNLGFGGIGESGMGQYHGKNSFDSFTHYKSIVKTPVWPDLPIRYHPYTKQNLNRL
                     KRFLK"
     gene            6114..>6386
                     /locus_tag="PKV59_02170"
     CDS             6114..>6386
                     /locus_tag="PKV59_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45588.1"
                     /translation="MSIIAAYIVPHPPIILPEIGKGEERKVQKTIDSYKKIAAEIAES
                     EPETIVITSPHATLYADYFHISPGHHAAGDFGQFGARGLVISADYDE"
BASE COUNT         1933 a         1265 c         1433 g         1755 t
ORIGIN      
        1 aatcttgaat aatgtcactt cactttcgac aacatttgtt caaaagagta tccgtcaaat
       61 gatacgagac ggacggctct ccatcatgaa agatcggctc cattttgcca acgaagccgc
      121 tcttgaaaat ttcctgtccc atattcaaaa tttcaataat caaattttgg attatgctat
      181 gaacaatcga aaactgatcc gaattttgat gaccgaatcc ttgaaggaaa gcaagcataa
      241 aaatgatttg attcaattta tcaaagagct tggaaaggca gccgaaaacc ccattattcg
      301 aaccattcgc gaagccaatg ccgattttcc tctgtcgaat aactttattt ttctgagatt
      361 cttctttcat cctgtttcga tcatcaattt tgctgtttac tatgagacgt atcaaaaaat
      421 ttgcggttta tccgaatcca aaatgcgaac ggctatgctc aattcgatcc ggacactttc
      481 aaattcattc gtttcgggaa acgatatcat tttacaaacg gaggcataaa acagaatttc
      541 attaatttat ttttaacgtc aattcaatga ccgacgaccg atgatttgta tagaaatggg
      601 aatagtaaag gaggaaaaat gaaaaatata caagattcaa tcgaagagct gattattcat
      661 tatgtcgtga cggatccgtt gggaaaactg cctaagatgc tcaacttggc ggaaaaaata
      721 gatcagggtc acctgcatga aaatgaaatt aatggtatcc gcaaaatcct ggatgataaa
      781 aacggtaact ggtacaaatt tattgaaggc atcagcgagg atatcgatcc gaaatgcctg
      841 caaaaagtga ttgaatgctt catgatcaat gccagtttga aaggtgaagc aaaggcgcga
      901 gcggtcgaga aaaaatatga tatgaacgtt ccgtggacga ttctggttga tccgaccagc
      961 gcctgcaatc tgcattgtac cggttgctgg gctgccgaat atgggaataa aaacaattta
     1021 tcttatgaga cgctcgattc gctcgtgaat cagggtgtcg acctgggaat ctatttttat
     1081 atcttttccg gcggcgaacc gcttatccga aaagatgata ttatccggct ttgcaaagct
     1141 caccaggatt gctatttctt ttcttttacc aacggaaccc ttgtggatga tcaattctgc
     1201 aaacagatgc tggaagttgg caatttcgct gtcgcctttt cgatcgaagg aaatgaagag
     1261 gcaacggata tgcggcgcgg taaaggaacc tatcgaaaag ttatcgccgc aatggacttg
     1321 atgaaaaaga ataaactgct gtttggctat tcaacctgct atcatcgcta taatacggag
     1381 agcgtcggct cggatgaatt tgtcgatgat atgattgccc gcggctgcaa attcgcatgg
     1441 aacttcactt atattccggt cggaaaggat gccgtccccg atctgctggc gactccggaa
     1501 caacgaaaat atatgtatca tcgcgttcgt gaaattcggg cgaccaaacc gcttttcgca
     1561 atggattttt ggaatgatgg agattctaca aacggctgca tcgccggcgg aagacggtat
     1621 ctgcatatta atgccgccgg agacgttgaa ccttgcgcat ttattcatta ttccaatgcc
     1681 aatattcatg atatgtctct gttggatgct ttgaaatcac cgatcttcgc ggaatatcgt
     1741 aaaggacagc cgtttaatga aaaccatttg cgaccttgcc cgctgttgga taatccgcag
     1801 gctttggcaa gaatggtgaa agcttccggt gcaaaatcga cggatatgga agctccggaa
     1861 gacgttgacg tattgacggc aaaaacgaaa gcggcagctg agaaatgggc accggttgcg
     1921 gatgaacttt gggaaaaaca aaataaagct aaggcagaaa aacaagccga agcgcaaaaa
     1981 gtggaaatag aagcctgagc caatcaaatg aaacggaaag cgatgaaata aggctttccg
     2041 aagcgattaa aaagaggagc gataaccgat tgaagggttt ccgtcatgag aattgctgac
     2101 tgtcgacaat tctcatgatg ttcctcttgt tttagaaggc tgctgaaaat attgaccaat
     2161 caattacgtc ggtttttacg catgatctcg gcttgacagg attgaaatcc ggcggtaaat
     2221 ttaaccaaac agttaaatta aaaggcggaa gaatggttac tcgggtaatt gaatcggaag
     2281 actcaaaaga tcgaatcata aaggcggccg ttcagctttt ttccgaaaaa ggatttgacg
     2341 gaacccgagt cagtgatatt gcggatttgg cggatgtcaa caaggcgctg atttattatt
     2401 actttactgg aaaagaagat attctggata tcttgctccg gcagttgttg aacgatatca
     2461 ccgctttttc aatggatttt atccacaccc accttatttc aatgatccgt gacggaacct
     2521 tggaactaaa agaagatcgg ctgacattca ttgataaaaa tgcggtggac tcttttctgg
     2581 acagcatgcg cggttattat cggaaggcgg tttcttatgc attggaaaat cggcaaatca
     2641 ttcgtattct gatgctggaa tcactgaaaa attccaaaca ccagaatgat ttatatcttt
     2701 tcctgaaatt gctcaataaa aatgacaaca atcccgttta taagacaatc ctaaatgccg
     2761 actggaactt taattattcc gatgaattta ttctgtataa attctttttc agtatgatgc
     2821 ctatcatcaa ttttgcggtt tattttgatg attacaaaaa tcattccgac atggaagaag
     2881 aggatttgat taaccgtttt ctgcaatcct gccaaacaac catcaatgca ttggtttgcg
     2941 ggaacgaaat attgatgcga aatgattgct cgattatcga aagcagataa tttttgtagt
     3001 agcgaataaa tagaaagggg taaattaaac caagcatgcc gttttagaaa tgttgacacg
     3061 tttcaatcgg ttgccggtaa gctaaggaga aaatgaatga atagtaaagt tcaaaatgca
     3121 atgctttcac tgttgatggg atacctggaa aatgatccga tgaacaaaat tcccacaatt
     3181 ctggatttag cggaaaaaat ggatacgggt catgttcatg ccaattcgtt taaagttgtt
     3241 cgcagagaat taaccgacaa aaacggtgtt tggtatcaat ttacagaaaa cctgtttaat
     3301 gaagtcgacc cgcgagtgat taaaaaagcg gttaccagtt ttttcttcaa tgggattctc
     3361 aataacggaa ctaaacaaga caaattacgt gaaaaatatc aatgtaatat tccctgggca
     3421 attttaatgg atcctaccag tgcctgtaat ttgcattgta cgggctgttg ggcagctgaa
     3481 tacggaaatc ggaataatct ttcctttgaa actctggatt caatctgtaa acagggaaaa
     3541 gagttgggaa tttatttcta tctgctgtca ggcggagaac cgaccgttcg taaagaggac
     3601 gtgattaaac tatgcgaagc gaaccccgat tgctttttct tcgcatttac aaacggaaca
     3661 ctggtggatg ataaattctg cgatgaaatg ttgagggtcg ggaatatcac tttgggtttc
     3721 tccatcgaag gagatgaagc ggcaacggat atgcgacgag ggaaagggac ctatcaaaaa
     3781 gtgatcgcgg caatggatcg catgaaaaaa cataagctcc ttttcggtta ttcgacctgc
     3841 tatcatcgtt acaataccga aagcgtcggt tccgatgaat ttgtggatga tatgatcgcc
     3901 cacggttgtc gctttgcctg gaattttacc tatataccgg tcgggaagga tgccgttacc
     3961 gatttgctgg cgacgcccga acagcgagaa tatatgtatc gccgcattcg cgaaattcgc
     4021 gcgaccaaac cgatttttgc attggatttc tggaatgacg gggaatacgt cggaggctgt
     4081 attgccggcg ggcggcgcta tctgcatatt aatgccgccg gtgatgtgga gccttgtgct
     4141 ttcattcatt attcaaatgt caatattcat gatgtaactc tgttggaagc tttgcaatca
     4201 ccgttattca tggcttatcg gcaaggacaa cctttcaacg aaaacctgat tcgcccctgc
     4261 ccgcttttgg ataatccgca ggcactttcc aaaatggtca aagtttccaa tgcaaaatca
     4321 accgacatgg aagcgccgga agatgtcgat gttttaacat cgaaaacgaa gccggcagcc
     4381 gagaaatggg cgccggtcgc cgatgaatta tggaaaaaac atttgaagga aaaagaagag
     4441 aaagaaaagg aaaaaattgc tggatgattc gaacccgcca acgttctcat ttttattctt
     4501 cctatcgaaa aatgctgccg aattatattt tggcagcatt tttcttatgg aaacattcga
     4561 taaaataaga tctgacgaca aaatatttat tgtcacacag aatcaatgaa tttcaggagg
     4621 gagctcgatg gatgaaatca aaacaatcgt tgatatgcaa cgtcgattct ttttaagcgg
     4681 aaaaacactt ccggtctctc agcggaaaaa ggcgctgcaa tctttgctga caatggtgga
     4741 tgaaaatcag caaatgatct ttgatgcctt gtttacggat ttacataaat ccgaatttga
     4801 aagttatatg accgaagtcg gaattgtttt aacggaaatt cgtcaggcga tgaaaaacct
     4861 ggacgcttgg acatccgtta aaaaagtccg gactccgctg tatctgttcc cttcaaaaag
     4921 cagcgtttat ccggagccct acggaaccgt cctcattatg gctccttgga attatccgtt
     4981 ccagttatgt cttgttccat taatcggcgc tttggcggcc gggaataccg ccgtcctgaa
     5041 accttccgcc tacgcaccgg cagtcagtcg ctgcatcgca gagttgattc accggtactt
     5101 tccttctgaa tatatagccg ttacggaagg aggacgggaa gaaaacagct cgttattgga
     5161 acaacatttt gacttgatct ttttcacagg tgggagtcgc atgggaaaaa tcgtcatgga
     5221 gtcggctgcg aagaatctga cgcctgttat tcttgaattg ggaggaaaga gcccgtgcat
     5281 cgtcgatcaa accgctgatt tgaaaaatgc gtcacggaaa attgttttcg ggaaattact
     5341 gaatgccgga caaacttgcg tggcgcctga ttatttattg gttcatgagt cggtaaaaga
     5401 tacgctcgtc cgtaatctta tcgaggaatt cgacaaagct ttccccggca aagactattc
     5461 cgattttccg gcgatcatca acgaaaaaca ttttgatcga ctgaaccggc ttatccggga
     5521 tgaaaatgtg atttatggcg gaaattcgaa tcgggatcgt cgctttttcg agccgacgat
     5581 tttattcccg gttcaatggg aaacaccggt catgcaagaa gagattttcg gtcccatatt
     5641 acccgttttg acttatgaga atttagacga tgtcatcgat cggttgcgaa aacttcccaa
     5701 accgcttgcg ctttatttat ttaccgaaga cagaaaaacc gaaaagaaaa taatcgaaaa
     5761 tgtagccttc ggcggcggtt gcatgaatga tacggttatg catttgtcca acccgaatct
     5821 cggtttcggc ggaatcggtg agagcggcat ggggcagtac catggaaaga acagttttga
     5881 ttccttcacc cattataaaa gtatagttaa aactcccgtt tggcccgact tgccgatccg
     5941 ttatcatcca tatacaaagc aaaatctaaa ccggttgaaa cggtttctca aataaatgcg
     6001 gtaaatccgc cattctattt cctgctcaaa aaaatcatcc ggatttttta atcttgccgg
     6061 ttgacgaaag ctatataatg aattaagaag atctttttca ggaggtaaaa attatgtcaa
     6121 ttattgctgc ttatattgtg ccccatcctc cgattatttt gccggaaatc ggaaaagggg
     6181 aagaacgaaa agttcaaaaa acaatcgaca gttacaaaaa aattgccgct gaaatagccg
     6241 aatcggaacc ggaaacgatt gtgattactt cacctcatgc cacactttat gcggattatt
     6301 ttcatatttc acccgggcat catgccgccg gtgattttgg ccaattcggg gcacggggat
     6361 tggtgatttc tgcggattat gatgaa
//
LOCUS       DAOMIV010000052         6339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_12418_length_6339_cov_4.163272, whole genome shotgun sequence.
ACCESSION   DAOMIV010000052 DAOMIV010000000
VERSION     DAOMIV010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12418_length_6339_cov_4.163272"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3164)
                     /gene="rpoC"
                     /locus_tag="PKV59_02175"
     CDS             complement(<1..3164)
                     /gene="rpoC"
                     /locus_tag="PKV59_02175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQP45589.1"
                     /translation="METKGLVALGISLASPETIRSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPTRDWQCYCGKYKNPRYKGIICDKCGVEVTRSSVRRERLGHIELATPIAH
                     IWYTRRIPSYLGLLLDISRRNLDRVLYFAQYIVTYVDEDARQKALNRLEDEISVSEKE
                     VAQEVNQKILGVKANRDKMLAQIQEQRDALLAKTDEMIAQKLDPVMKEGQRLEAELQE
                     KIGQVINSDLVFSSTGTAIASAGETISNQHISLVQKTVKEKLDEIENDFKGQLQRDIE
                     RLDMDKQQVRAEAEEQMEALRNQLADESAESQNQNSKMRDELQELQPLTFMSETSYRE
                     LKSRWGNVFRADMGAEAFYDILKRLDLDQLSKDLWNEVRTTKSKQKRKKATSRLKVVE
                     SFRSSKNHPEWMILTVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPKLKLYQCGLPKTMALELFHPFLVSRLVEHGYATNVKGANR
                     IIERNRPEIWEVLEEVISERPVLLNRAPTLHRLGIQAFEPILIEGNAIQLHPLVCTAF
                     NADFDGDQMAVHVPLSEKAVEEARTLMLASKNLLKPADGEPIISPSKDMVLGIYYLTM
                     DSDGDHPGDGRAFYSIDEVQYAYDLDQVKLHTKIKFLTDTWFGKDGARLAKPEKRIID
                     TTIGRVIFNNILPEEIRFKNSPLDKGAIKDLIAEVYEICGQDVTTEVADSIKNTGFEF
                     AMRSGSTIAVSDITIPDTKDALLAEADNEVESINRQWRRGLLTEQERNDRTIDVWQET
                     TNKIASEVRKSMDPQGNMAIMATSGATKGGFAPIAQLAGMRGLMADPSGRIIRLPIKS
                     NFREGLDALEYFISTHGARKGLADTALRTADAGYLTRRLVDIAQDIIINEEDCGTEEG
                     IWIRRSDDVAKQAFHERIYSRVLAEKVIDPETGEILAEKGEIIDHDLAHKISDTKSIP
                     EIKVRSPMTCELKHGICAKCYGID"
     gene            complement(3364..4752)
                     /locus_tag="PKV59_02180"
     CDS             complement(3364..4752)
                     /locus_tag="PKV59_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQP45590.1"
                     /translation="MDQKTDNKIKTILLTVQILKPATIRLILNENAGQMENEELKTWF
                     RGHNSTSLQEADVQFYQIMKNQSRIVIPSINQSRLLAGLGMEDSGAIILPVRTDFGYC
                     GCFWGCFPEASFDDTLIETFANFNDWVIEVIGNTQKNDFGVEAIAKRYADFLDQQKTP
                     ALIVIYPDQISFSNPSFEAMSTKENVLQMIRKNISVDSSFMELVDLYHCRIRDVNFPG
                     GKTGKIFQFVSATENSVPVSFDANELEYFSLLVEKAGGNLELLDKAGNMSLIQFNYKE
                     KAEAQLKRLNTLIRYEQKHYKNFLNSREGSFEIINIGDLVKDVVFDLISVAKKKNLDI
                     NYDSEKVDNSQTQSGKVIGDPWLLSLSIFNLLSNAIRYSKPDSKSIQVDLTYQVDSWI
                     LRIQDFGIGISPLDVEKILSDTPDRSPAQDTQAVHGLDFVKYVVKLHRGRFNLESKLG
                     KGSTFTLDIPYY"
     gene            complement(4792..5463)
                     /gene="cmk"
                     /locus_tag="PKV59_02185"
     CDS             complement(4792..5463)
                     /gene="cmk"
                     /locus_tag="PKV59_02185"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQP45591.1"
                     /translation="MKKPLQIAIDGPAASGKSTVGKKIAENLNYLFFDTGLMYRVATY
                     AVLEQQCPLDDEGKLADIISRADIELQNNSQTGETDVLLDGKTITDLLHLPQVDANVS
                     LIAAIPAVRKLLTAQQRKIALQGKIVIAGRDIGTVVLPEAPLKIFLLASAEKRAEPRY
                     LENLRNGIPSDYDEILYNIKQRDQIDSNRNVAPLKPAEDAILIDTDSLTQNQVADKIM
                     SLIDQ"
     gene            complement(5482..>6339)
                     /locus_tag="PKV59_02190"
     CDS             complement(5482..>6339)
                     /locus_tag="PKV59_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45592.1"
                     /translation="WISLPLWFFSTDVILSLRPIKKTEIDLPMAIAILIPPILSFFLL
                     LRLSEMLRMGDLSLPLQFTWQNQAVSTPFSRLQAYLLIIVICIIIFAVLLPMLLDYFS
                     PGKIKKGMLYGCLSVLLVCTFGSSWKAAGFSNTKDDPFLHTSASRHELILGNQIVQIE
                     NQIIPIIQEIGWKATGFIHQASGIILVDNDPMLRWTLQYYPDVVFTDVLDIRTNSPSF
                     VITEGSEYEPLWASYVGLVSDWKTTDHWEMYRTNDWLSWILYRKPVISGQKLVIWQQS
                     RFLTKTNLE"
BASE COUNT         1683 a         1494 c         1329 g         1833 t
ORIGIN      
        1 aaatcgatac cgtagcattt cgcacaaatt ccatgcttta actcgcaggt cattggtgag
       61 cgaaccttta tttccggtat gctcttcgta tcagaaattt tatgagccag atcgtggtcg
      121 attatttcgc ctttttcggc gagaatttcg ccggtttccg gatcaatcac tttttccgcc
      181 agtacccggc tgtaaatacg ttcgtggaaa gcctgtttcg ccacatcatc ggaacgccga
      241 atccagatac cttcttctgt tccgcaatct tcttcgttga taataatatc ttgcgcgata
      301 tcgaccaaac gacgggtcag ataaccagcg tcagcggtcc ggagggcggt atcagccaac
      361 cctttccgag ctccgtgagt ggaaatgaag tactccaacg catccaaacc ttcacggaaa
      421 ttggatttaa ttggcagccg aataatacga ccggacggat cagccatcag cccgcgcatt
      481 cccgctaact gtgcgatcgg tgcaaaacca ccttttgtcg cgccggaagt ggccataatc
      541 gccatgttcc cctgcggatc catgctcttg cggacctcgg acgcgatttt attggtagtt
      601 tcctgccaga catcgatcgt gcgatcgttt cgttcctgtt ccgtcagtaa cccgcgccgc
      661 cattggcggt ttatcgattc gacttcgttg tcggcttccg ccagtaatgc atcctttgtg
      721 tcgggaatcg taatatccga tacggcgatc gtcgaaccgg agcgcatcgc aaattcaaag
      781 ccggtgttct taatcgaatc ggcgacttcc gttgtgacat cctgaccgca aatttcatat
      841 acttccgcaa tcaaatcttt aatggcgcct ttatccaacg gtgaattttt aaagcgaatt
      901 tcttccggta atatattgtt aaagataacc cgtccgatgg ttgtatcgat gattcgtttt
      961 tccggtttcg ctaagcgagc gccgtctttg ccgaaccagg tatccgtcag gaatttaatc
     1021 ttggtatgga gcttgacctg gtcgagatca taggcatatt ggacttcatc gatagagtaa
     1081 aaagcccttc cgtcacccgg atgatccccg tcactgtcca ttgtcaaata ataaataccg
     1141 agcaccatat ccttggatgg gctgatgatc ggttctccgt ccgccggttt gagtaagttt
     1201 ttcgaagcca gcatcaatgt acgcgcttct tcaacggctt tttcggaaag cggtacatga
     1261 acagccattt ggtcaccgtc aaagtccgcg ttgaaagcgg tacaaaccaa cggatgcagt
     1321 tgaatcgcat ttccttcgat caaaatcggt tcgaaggctt gaatacccaa ccggtgaagc
     1381 gtcggtgcac gattcaacaa aacaggtctc tcgctgatca cttcctcaag aacttcccag
     1441 atctccggcc gatttctttc gatgatacga tttgcgcctt tgacattggt ggcataacca
     1501 tgttccacca ggcgagaaac gaggaaagga tggaataatt cgagcgccat tgtttttggc
     1561 agaccgcatt gatacaactt caatttcggt ccgacgacaa tcaccgaacg accggaataa
     1621 tcgacccgtt tcccgagcag atttctgcgg aatcgtccct tcttaccctt cagcatatca
     1681 ctgagagatt tcaattcgcg gcgaccgcgg cgtgacaacg ctttcccgcg ttgtgagtta
     1741 tcgatcagcg aatccaccgc ttcctgcaac attctttttt cgttccgaac gatgacatcc
     1801 ggtgcaccca gttccaacag ccgcttcaaa cgattgttcc ggttgatgac ccgccggtat
     1861 aagtcgttca gatcggatgt ggcaaaacgt ccgccgtcca gctgaaccat cggacgcaaa
     1921 tccggaggga tgaccggcag aacggttaag atcatccatt ccggatgatt cttcgaagaa
     1981 cggaaagatt caacgacttt gagacgtgac gttgctttct tgcgtttttg tttactcttg
     2041 gttgttcgaa cttcgttcca taaatctttt gaaagttgat ctaaatccaa ccgtttcaaa
     2101 atatcgtaaa acgcttccgc gcccatatcc gcccgaaaaa cattccccca gcggcttttg
     2161 agttcacggt atgaagtttc actcatgaaa gttaacggtt gtaattcctg aagctcatcc
     2221 cgcattttcg agttttgatt ttgcgactcg gctgattcgt ccgcgagttg attgcgaagg
     2281 gcttccatct gctcttccgc ttcggcgcga acctgctgtt tgtccatgtc caaccgctcg
     2341 atatcgcgtt gcagttgtcc tttgaaatca ttttcgattt catccaattt ttcttttacc
     2401 gttttttgaa caagagaaat atgctggttg gaaattgttt cgccggcgga agcgatagcg
     2461 gtaccggtgg atgaaaaaac caaatccgag ttgataacct gaccgatttt ctcctgtaat
     2521 tccgcttcca agcgctggcc ttctttcatt accggatcca gcttttgagc aatcatctcg
     2581 tccgtttttg ccaacaaagc atcccgttgt tcctggattt gggcgagcat cttatcgcga
     2641 ttcgctttta caccgagaat tttctgattg acttcctgag cgacttcctt ctccgacaca
     2701 ctgatttcat cctcaagccg attcaatgcc ttctgccggg catcttcgtc gacataagtg
     2761 acgatatatt gcgcaaaata aagaacacga tccagatttc ttcttgaaat gtccaaaagt
     2821 aatcccaagt atgaaggaat tctccgggta taccagatgt gagcgatcgg ggtagccaac
     2881 tcgatatgac cgagtcgttc ccgccgaaca gaagaacgag ttacttcaac gccgcattta
     2941 tcacagatga ttcctttata acgtggattt ttatatttcc cacaatagca ttgccagtca
     3001 cgcgtcgggc caaaaatagc ttcacaaaac aatccgtcct tttccggtcg aagacgtcga
     3061 tagtttattg tttcgggctt taaaacttca ccgtaagacc atgaccgaat tgtttcaggt
     3121 gaagcgagac tgattcctaa tgcaaccaaa cctttagttt ccacgaattt tcctcccgag
     3181 agtttgagtg tttcccattc attaagcatt caatgcatag cttctttttc tctatgcata
     3241 aaaggagtta ttaaaagctc attcggcttt aaatgcatta aagccgattt atcattataa
     3301 cacaacagaa agattcctcc gctatattta agcggctttt cgaaaaatgt gacaagaatg
     3361 ttttcaataa tatgggatat ccagggtgaa agtcgaacct tttcctaatt tactttccag
     3421 attaaatctc ccacggtgta attttactac atattttacg aaatccaaac cgtgaaccgc
     3481 ctgggtatct tgtgcgggtg atcgatccgg tgtatcactg agaatttttt caacatccaa
     3541 cggagaaata ccgattccga aatcctgaat tcgcagaatc catgaatcca cttgataggt
     3601 caaatccact tggatcgatt tgctgtcggg tttggaataa cgaatcgcgt tggataataa
     3661 attaaaaatc gaaagggaaa gcagccaggg atctccgata acttttccgc tttgcgtctg
     3721 gctgttatct actttttcgg agtcgtaatt gatatcaagg tttttctttt tggctacgga
     3781 tattaaatca aaaacaacat cttttactaa gtcaccgatg ttgataattt caaaagatcc
     3841 ttcgcgtgaa tttaaaaagt ttttataatg cttttgttca taccgaatta gtgtattgag
     3901 gcgttttaac tgcgcttccg ctttttcctt atagttaaat tgtatcaacg acatatttcc
     3961 ggctttatca agcaattcca agttcccgcc ggctttctcg accagtaatg agaaatattc
     4021 cagttcgttc gcatcaaaac ttaccggaac ggaattctcc gtcgccgaaa cgaattgaaa
     4081 gattttaccc gttttcccgc cgggaaagtt gacatcgcga atgcggcaat ggtacaaatc
     4141 caccagttcc ataaaggagg aatcgaccga aatatttttc cggatcattt gaagcacatt
     4201 ttcttttgtg ctcattgctt cgaaagaggg gttggaaaag ctgatttgat ccggatagat
     4261 gacaattaaa gcaggcgttt tttgttgatc caaaaaatct gcatagcgtt tggcgatcgc
     4321 ttccacacca aagtcattct tctgagtatt tccaatgact tcgatgaccc aatcgttgaa
     4381 atttgcaaaa gtttcgatta aggtatcatc gaaagaagct tcgggaaaac atccccaaaa
     4441 acaaccgcag taaccgaaat ccgttcgaac cggcaaaata atcgcacccg aatcttccat
     4501 ccccaatccg gctaacagcc gcgactgatt gatcgaggga atgacaatgc gggattggtt
     4561 tttcatgatt tgatagaatt ggacatccgc ttcctgaaga gaagtactgt tatgcccgcg
     4621 aaaccaggtt ttcagctctt cattctccat ttggccggca ttttcattta atattaaacg
     4681 gatggtggcc ggcttcagga tttgaacggt caaaagaatt gttttgattt tattatctgt
     4741 tttctggtcc aaattccttt tccagtcttc gagatgtcta ttttatatat tctattgatc
     4801 aatcaatgac attatcttat cggcaacctg gttctgcgtc aacgaatcgg tgtcgatgag
     4861 aatcgcatct tcagccggtt taagcggtgc gacgttacgg ttagaatcga tttgatctcg
     4921 ctgtttaata ttatacagaa tttcatcata atccgacggg ataccgttcc tgagattttc
     4981 caggtagcgc ggttccgccc gcttttcagc ggatgcaagc agaaaaatct tcagcggtgc
     5041 ttcgggcaaa acaacggttc cgatatcccg cccggctata acgattttac cttgaagagc
     5101 aattttcctc tgttgagcgg tcaataattt tctgacggcg ggaatggcgg caatcagaga
     5161 aacatttgca tcgacttgag gtaaatggag aagatcggtt attgtctttc cgtccagcaa
     5221 aacatccgtt tcacctgtct gcgagttatt ttgcagctcg atgtctgctc gtgaaatgat
     5281 gtctgctaat ttcccttcgt catcgagcgg gcattgctgt tccagaaccg cgtaagttgc
     5341 cactcgatac atcaaaccgg tgtcaaaaaa aaggtaattc aaattctcgg caattttctt
     5401 cccgactgtt gattttccgg aagcggccgg tccgtcaata gcaatttgga gaggcttttt
     5461 cattaaagca ggtgtctctt ttcattcaag atttgtcttt gtcaaaaaac ggctttgttg
     5521 ccatataaca agtttttggc cggaaattac aggcttccga taaaggatcc aggaaagcca
     5581 atcatttgtt cgatacattt cccaatgatc ggtagtcttc caatcggata ccagcccgac
     5641 atatgaagcc cacagaggtt catactcaga tccttcggta atcacaaaag acggagaatt
     5701 tgtacggata tccagaacgt ctgtaaaaac gacatcagga taatattgca acgtccatcg
     5761 cagcatcgga tcgttatcga ccaaaataat tccggatgct tgatgaataa atccggtagc
     5821 tttccaaccg atttcctgaa ttatcggaat tatttgattc tctatttgaa caatttggtt
     5881 tcccaaaatc aattcatgcc ttgaagcgga agtatgaaga aagggatcat ctttggtatt
     5941 gctgaatccc gcagctttcc aagaacttcc gaatgtacag acaagcaaaa ccgatagaca
     6001 accgtataac attccttttt ttattttccc ggggctgaaa taatcaagca acatagggag
     6061 taaaacggca aaaatgatta tacaaataac aataatcaag agataagcct gcagtcgact
     6121 aaacggtgta gagactgcct gattttgcca ggtaaattgt aacggtaacg acaggtcacc
     6181 cattctaagc atttcactca aacggagcaa taagaagaag gacaaaatcg gcgggattaa
     6241 aattgcaatc gccatcggca gatcgatttc agtcttctta atgggtctga gcgataaaat
     6301 gacatccgtc gaaaaaaacc atagaggaag gctaatcca
//
LOCUS       DAOMIV010000053         6303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_12519_length_6303_cov_4.288252, whole genome shotgun sequence.
ACCESSION   DAOMIV010000053 DAOMIV010000000
VERSION     DAOMIV010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12519_length_6303_cov_4.288252"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKV59_02195"
     CDS             <1..404
                     /locus_tag="PKV59_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45593.1"
                     /translation="DLPTEAERREIMMVHILKRKRLPEAFDLDRLAKASEGYVGSEIE
                     QAIIDAMYIAFNDGEREFTTEDILAALKRQVPISVSQRENIERLRLWLREGRAQSASF
                     RDTSTAESQFVPIQIELDQPPKNESCPTSLL"
     gene            383..760
                     /locus_tag="PKV59_02200"
     CDS             383..760
                     /locus_tag="PKV59_02200"
                     /inference="COORDINATES: protein motif:HMM:NF018565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HQP45594.1"
                     /translation="MSHISVIKTHLQNREFLIQALKELGYQIVEGDNLSVQANNRKKK
                     VDFLIEIPYSAPIGFRESKNGYQIVADWFKVLTDRNTFRNQLVQKYTYLYLKSELDKQ
                     GFEISHEDMSEKNQIHLVVRRTV"
     gene            761..2986
                     /locus_tag="PKV59_02205"
     CDS             761..2986
                     /locus_tag="PKV59_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HQP45595.1"
                     /translation="MDSSKIYRNYLELLKSPVVPQGFPTDQLYQFACTGAIASLSQDI
                     RENPDSKTEAAVSALKEIVSHAAAERFRESALEALKEAALSGNPMCRDALFQLATVEL
                     NPAALKILEEYNLSSPSVDFESAKYFFLKNKNDLLRIDPTLNELTRFYLAAPRSVRQE
                     MQRIARIMLPNWGNIVEFLQDPKAETSAWEKIQSEFLYFSETEKELLIRQLLGQSVDA
                     QKLIADLFLRYDDPALLRACLSNQLEPSRADRKALFYFLSDQWTQYEQNDLDYQWIRK
                     AFSEGSDFLRNRLIRHGLKSGHTSWLETSEFSVQTEHVFRPISFSQWESWVAKLIAQS
                     DWERLWQILPIAPVALVPEVIAALQRENFKPQSEEERDFFRKLIQICSELPPSIPVPF
                     VRRFYNQTPQPFLLNLSKDGNYLAAAFLNGDIRFWNLGDRTLSSGSLQIPNLSPKAFC
                     FGENAETLASAWSDNVIRIFRFPSGVLLNKLPPTAAPVTNLFLSVDGRRIYATEQPNF
                     LQAWGYPAGIPLDDIKLPGFQIIRSSVCPENNRLIFSDDHQSLFIFDIRKKQTVAEIN
                     PFPPQSVLDTSSELLTCVSLDQHITVWNLNSQMKLSDVQESGFSGKILSIAQLIPGEV
                     AVFGSQIGECAVCSLHSGKHLAEIHGNDPFSSLSAIRINTQDNQLYLADSDGSITQWD
                     LTLLRQTISIFHPETIPNPEKLRTYQQKFSTPAVQKTIALMNILFDWRTRYDIEVEFD
                     E"
     gene            2979..3938
                     /locus_tag="PKV59_02210"
     CDS             2979..3938
                     /locus_tag="PKV59_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45596.1"
                     /translation="MNERNPISVPNKSLNQLSLTGHVAFLQNMIQEAAEAASLAPQCL
                     IGLQRVLKYEYHLHGDGFKNQAGTDTPSLSVFKHWLIFLLTGYNLNIHIRFVENILSA
                     CKRSRTLHTATLKIYVYPSAKIFDFNQIGQDATLKIHEALIGMPESEIDDFIDKLADK
                     NRTELYRLVRKSFNEEPALQIRQCFQKELPEKKKKGRPVGKFFNLNKIFASVNQEYFG
                     SKLLCPVLKWSAQENRRRMGSYNLRTDTIIVNRALDQIDTPLFVIRFVMYHEMLHKFV
                     GIKRKNGRNYAHTSKFRNYEKQFAEYAEAKEYLSHLRIDQHKK"
     gene            4003..5397
                     /locus_tag="PKV59_02215"
     CDS             4003..5397
                     /locus_tag="PKV59_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45597.1"
                     /translation="MKKIWLAILVFAVFLAAGIPSLSVAAQNTAYQKDNSFFSEEDVS
                     SILGGVTNLIQSFNAEAVYDGLKAALDMSKLFGFWETPYFENIQNFMITKGQEQISNF
                     AQEDYLEQLIAKAYADQNKPNHNKSSFSKNSGFLITGPDSFEIQMSAKGTVKGNHISL
                     FSFDGTPLTEIDLSVNPTDPNQLIIYGMDMADQSSKTYYLEISNREISLDSDTDSIFS
                     LWFRPGERILIEIPSSESEVELAFDKKAYKITGTVSSPEQTMEIELELNPLKNLIALA
                     LNGTPVAWIESDPVKNSLDLVFADAELLMEFDDDTHEILGTFYTTDQQSQFKLICDKP
                     SNSLALEMVIEGEMTELFKLAFSKQANGVSLNSAGGLIFSATIDQEKGTMTINDSSGF
                     RILDESQILDLINSSIKTGPEAETEVVDNFLNDEEETAVPEEEIIPTAAPISSPTEIP
                     TTVPVAVPTLDAGI"
     gene            5588..>6303
                     /locus_tag="PKV59_02220"
     CDS             5588..>6303
                     /locus_tag="PKV59_02220"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQP45598.1"
                     /translation="MKLAIPLILQYLLSSSVTLVDNIMVGALGDVSLAAVNICTQFYL
                     KLFHIVIFGLVAGASILTTQYWGVHDSKNIHRIMGLEMIVGSGLGVLFSILTAFFPEK
                     ILGFYSRDPQVIQAGSLYLSTFSLIFLLHPIALIFASAHRAIGDTKLPMIAIALSLAI
                     KLILNYFLIYGNWGFPKLGIAGAAYATLIAKISETALLLFFTYKSKSPVAATFNTLFD
                     FDKKLIIKTTKNVLPVLINET"
BASE COUNT         1803 a         1409 c         1361 g         1730 t
ORIGIN      
        1 tggatttgcc caccgaagcg gaacgccgcg agataatgat ggtgcatatt ctgaagcgca
       61 aaaggctacc cgaagccttt gatctggatc gattggcaaa agcctccgaa ggatatgtgg
      121 gatcggaaat cgaacaagcg attattgacg ccatgtatat cgctttcaac gatggggaac
      181 gcgaattcac aacggaagat attttggcgg cattgaaacg tcaggttccg atttcggttt
      241 cacagagaga aaatatcgag cggttacgcc tgtggctgcg ggaaggaagg gcacaatcgg
      301 cctcgttccg cgatacctct accgccgaaa gccagtttgt tccgattcag atcgaattgg
      361 atcaaccgcc gaagaacgaa tcatgtccca catctctgtt ataaaaacgc acctgcaaaa
      421 tcgcgaattc ctgatccagg cgttgaaaga gcttggttat cagattgttg agggggataa
      481 tttatccgtt caggcaaata atcggaaaaa aaaggtcgat ttcctgatag aaatccctta
      541 ttcggctcca atcggttttc gggagagcaa aaacggttat caaatagtgg cggattggtt
      601 caaagtcctg acggatcgga atacttttcg caatcagctg gtgcaaaaat acacttacct
      661 gtacctcaaa tccgaactcg ataagcaagg attcgagata tcccacgaag acatgagtga
      721 aaaaaatcaa attcatttgg tggttcgtcg tacggtctaa atggattcat caaagattta
      781 ccggaattat ctggaactgc taaaatcccc ggtcgttccg cagggatttc ctacggatca
      841 gctctatcag ttcgcctgta ccggggcgat tgcaagtctc tcacaagata ttcgtgagaa
      901 tccggattcg aaaacagaag ccgctgtatc cgccttaaaa gaaatcgtat cacatgccgc
      961 ggcggaacgt ttccgtgaat cggctttgga agcgcttaag gaagccgctc tttcaggaaa
     1021 tccgatgtgt cgcgatgcac tttttcagtt ggctactgtc gagttgaatc ccgccgcact
     1081 gaaaattttg gaagaatata atttgtcctc gccgtccgtc gattttgaaa gcgcaaaata
     1141 cttttttctg aaaaataaaa acgatctgct gcgaatcgat ccaactttga atgaattaac
     1201 ccgtttctat ttagccgctc cacgcagtgt ccggcaggaa atgcagagaa tcgcgcgtat
     1261 tatgttaccg aattggggga acattgtcga gtttttgcaa gacccgaagg cggaaacttc
     1321 cgcatgggaa aagatccaat cggaattcct ttatttctct gaaaccgaaa aagaattgct
     1381 cattcggcaa ttactcggac aatcggtaga cgcacaaaag ctgatcgccg atctatttct
     1441 gcggtatgat gatccggctt tgctccgagc ctgcctgtcc aatcaattgg aaccctcgcg
     1501 cgccgaccga aaagcattgt tctatttttt aagcgaccaa tggacacaat atgagcaaaa
     1561 cgacctggac tatcaatgga ttcggaaagc gttttcggaa ggatcggatt tcttacggaa
     1621 ccgactgatc cgacacggtt tgaaatccgg tcacacctca tggcttgaaa cttcggaatt
     1681 ttcggttcag accgagcatg tttttcgtcc gatttctttt tcgcagtggg aaagctgggt
     1741 tgccaaatta atcgctcaat ccgactggga acggctctgg cagattctgc caattgcacc
     1801 ggtggctttg gtcccggagg ttatcgccgc cttacaacga gaaaatttta agccgcaatc
     1861 ggaagaagaa agagattttt ttcggaaatt gattcaaatc tgttccgaac tgccgccatc
     1921 gatacccgtt ccgtttgtcc gtcgatttta taatcaaaca ccgcaaccgt tcctgttgaa
     1981 tctttcgaaa gacggcaatt atctggcggc ggcttttctg aacggcgaca ttcgattctg
     2041 gaatctgggc gatcgaacgt tatcttccgg ttcccttcaa atccccaatc tcagtccgaa
     2101 agccttttgt ttcggagaaa atgcggaaac gctcgcctca gcttggtcgg acaatgtgat
     2161 tcgtattttt cgttttcctt cgggcgtgct gttgaacaaa ctgcccccca ccgccgcacc
     2221 cgttacaaac ctgttcttga gtgtcgacgg tcgtagaatt tatgcgacgg aacaaccgaa
     2281 ctttttacaa gcttggggat atccggcggg aattcctctt gatgacatca aattaccggg
     2341 ttttcagata atccgatcct cggtttgtcc ggaaaacaat cggctgattt tttcggatga
     2401 tcatcaaagc cttttcattt ttgatattcg aaaaaagcaa accgttgcgg agatcaatcc
     2461 gtttccgccg caatcggtgt tggatacttc atccgagcta ttaacttgtg tttcccttga
     2521 tcagcatata actgtttgga atctgaacag ccaaatgaaa ctttcggacg tacaagagtc
     2581 ggggtttagc ggaaagatcc tgtcaatcgc tcaattgatt cccggcgagg ttgccgtctt
     2641 cggttcgcaa atcggagaat gcgcggtttg ttcgctgcac agcggcaaac atttggcgga
     2701 aattcacggc aacgaccctt tttcttcttt atcggccatc cggatcaata cgcaggacaa
     2761 tcagctttat ctggccgact ccgacggaag catcactcaa tgggacttga cattgctcag
     2821 gcagacgatc agcatctttc atcccgaaac gattcccaat cccgaaaaac tgcgtaccta
     2881 tcaacagaaa ttttccacac cggccgtaca aaaaacgatc gcattgatga atatactgtt
     2941 tgattggcgt acccgctatg acatagaggt ggaatttgat gaatgaaaga aacccgattt
     3001 ccgttccgaa taagagtctg aatcaattat ccctcaccgg gcacgtcgct ttcctccaaa
     3061 acatgattca agaggctgcc gaagcagcgt ctttggctcc tcagtgtttg atcggtttac
     3121 agagggtgct taaatatgaa tatcatttac acggagacgg attcaaaaac caagccggaa
     3181 ccgacacccc atccctttct gtatttaaac attggttgat ctttctgttg accggatata
     3241 atctcaatat tcatatccga tttgttgaaa atatcctttc cgcatgcaaa cgctcaagaa
     3301 cacttcatac cgcgactctt aaaatatatg tctatccttc cgcaaagatc tttgacttta
     3361 atcaaatcgg tcaagatgcg acattgaaaa ttcatgaagc actcatcgga atgcccgaat
     3421 cggaaatcga tgattttata gataaattag ccgataaaaa tcgtaccgag ttatatcgtc
     3481 tggttcgaaa atcttttaat gaggaacccg cattacaaat ccgacagtgt tttcagaaag
     3541 agcttccgga aaaaaagaaa aaaggtcgtc cagtcggaaa attctttaac ctcaataaaa
     3601 tattcgcatc ggtcaatcaa gaatatttcg gatcaaagtt attatgcccg gttctaaagt
     3661 ggtcagctca agagaatcga aggcggatgg gttcttataa tcttcgaacg gatacgatta
     3721 tcgtgaatcg agcgttggac caaatcgata cgccgctttt tgtcatccgt ttcgttatgt
     3781 accatgaaat gcttcataaa ttcgtgggaa taaaacggaa aaacggacgg aattatgcac
     3841 atacatcaaa attccgcaat tacgaaaaac aattcgccga atatgccgaa gcaaaagaat
     3901 atctttccca cctgagaatc gatcaacata aaaaataaat ttgtgatatt aattgactac
     3961 taaagttttc tttttttcgt gaattacaaa gaggaaaaaa atatgaaaaa gatatggctt
     4021 gctattcttg tctttgcagt ctttcttgct gccggaattc cttccttgtc tgtcgctgct
     4081 cagaatacag cctatcagaa ggataattcc tttttttcag aagaagatgt cagcagcatc
     4141 ttaggaggtg tcactaacct gatccaaagt tttaatgccg aagccgttta tgacggactg
     4201 aaagctgcgt tggatatgtc caaactgttc ggtttttggg aaacgccata ctttgagaat
     4261 atccaaaatt ttatgatcac gaaagggcaa gaacagattt caaacttcgc ccaagaggat
     4321 tatttggagc aattgatcgc aaaagcttat gccgatcaaa acaaaccgaa tcacaataaa
     4381 tcatcctttt caaagaattc aggctttttg ataaccggac ccgatagttt cgaaatccaa
     4441 atgtcagcca aaggcacggt taaaggaaac cacatttctc ttttcagttt cgacggaaca
     4501 cccttgaccg agattgacct gtccgttaat ccgaccgatc ccaatcaatt gataatctat
     4561 ggaatggata tggcggacca aagcagtaaa acctattatc tggaaatcag taatcgggag
     4621 atatccttgg attcggatac ggattctatt ttttcattgt ggtttaggcc aggagaaaga
     4681 attcttattg agattccttc ctccgaaagc gaagtggaac tggcttttga taaaaaagca
     4741 tacaaaatca ccggcacggt cagctcaccg gaacaaacca tggagattga attggaattg
     4801 aacccgctga aaaatctgat cgctttggcg cttaacggca ctccggtcgc ttggatcgaa
     4861 tccgatccgg tcaaaaacag tttggatctt gtattcgccg atgccgaact tctgatggaa
     4921 tttgatgacg atacacatga aattctcggc acattctata caaccgatca gcaatcgcag
     4981 ttcaaactga tctgtgataa accgagtaat tcgctggcgt tggaaatggt gattgaagga
     5041 gagatgacag agctcttcaa gcttgccttc agtaaacagg caaatggtgt ctcactgaat
     5101 tcggccggag gattgatttt ttccgctaca atcgaccaag aaaaaggtac catgacgatc
     5161 aatgattctt cgggcttcag aatattggat gaatctcaga tccttgattt aatcaattct
     5221 tccataaaaa caggaccaga ggcggaaacg gaagtcgtcg ataatttctt gaatgatgag
     5281 gaagagaccg ctgtcccaga ggaagaaata attccgacgg cagctccgat ctcatcccct
     5341 acggaaatac cgacaacggt tcccgtagcg gtccccacat tggatgcggg aatataaatc
     5401 gggggaaaga tcaattaaaa tagtttgaaa tatcttacaa ttttataagg aactgagctg
     5461 cgccggcgaa aggtcatatt tatcagctca gtttcttttt gcaaaatgct cggaggtttc
     5521 aatccgtgca gtcagtgagg tcatgataaa aatcaaaaaa ccagacagaa tctattttcg
     5581 gcagatcatg aaacttgcaa taccgttgat cctccaatac ctgttatctt cttctgtaac
     5641 attggtggat aacatcatgg ttggagcatt gggagacgtt tctctggcgg ctgttaacat
     5701 ttgcacccaa ttttatctaa agttattcca tatcgtgatt ttcggtttgg tcgccggtgc
     5761 ttcgatcctg acgactcaat attggggcgt tcacgactca aagaacatcc atcgcattat
     5821 ggggcttgaa atgatcgttg gttccggact cggtgtttta ttttcgatct taaccgcctt
     5881 tttcccggag aagattctgg gattctattc ccgagaccct caagtcattc aagccggatc
     5941 gttatacctg tccacctttt cgctgatctt tttactccat ccgattgcat taatcttcgc
     6001 gagtgctcat cgggctatcg gtgatacaaa attaccgatg atcgctatcg ctttatcatt
     6061 ggcaattaag cttatattaa attactttct catttacgga aattgggggt tcccgaaact
     6121 gggcattgcc ggagcggctt atgcgacttt aatcgcgaaa atttcggaaa ccgcattatt
     6181 gcttttcttc acttacaaat caaagtcccc ggtcgctgca accttcaata cattatttga
     6241 ttttgacaaa aaactaatca taaaaacaac caagaatgtg ctccccgtat tgattaacga
     6301 aac
//
LOCUS       DAOMIV010000054         6260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_12634_length_6260_cov_4.605963, whole genome shotgun sequence.
ACCESSION   DAOMIV010000054 DAOMIV010000000
VERSION     DAOMIV010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12634_length_6260_cov_4.605963"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKV59_02225"
     CDS             <1..326
                     /locus_tag="PKV59_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQP45599.1"
                     /translation="VVHLARYSPRKGTLSERTMLDDVSDEEKWRRFRTVETLQERIAT
                     ELHAALEGKILPVLFEEKQKGRWMGRTEHMDLVYVESESDLTGLILPVKMNWTGPWTM
                     IGELV"
     gene            346..2766
                     /locus_tag="PKV59_02230"
     CDS             346..2766
                     /locus_tag="PKV59_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HQP45600.1"
                     /translation="MDKKSLKMLEYPEVIRRLAQYASFNVSEQMAQNLHPETDSSIIR
                     YKLSLTTEARKLLSVNDTFRLGGCSDLRPYLEVARKQGTLEAKAFVEIAYTLSVARDL
                     YRSLSHHEAEYPNLAAIAENLIPPAGLIERINFTVNDRGEVLDSASEKLTSIRREIRL
                     SYNRLLGRLEKMVKESRYQPMLQEPIITQRNGRYVIPLKAEYKGQLKSIIHDQSSSGA
                     TLFVEPLATVELNNVYQALQLDERNEIIRILQNLTAFVGDYADQIEQSVEALAEIDFA
                     MMCAKYADDLRAAEPLLVDPYKKTGNPVIKLYQARHPLLPVETAVPIDVVPDEETFAV
                     VITGPNTGGKTVSLKTIGLLILMAQSGLHIPVRSGSEFSVFKNVFADIGDEQSIEQSL
                     STFSGHITNIIRILKEADSKTLVLLDELGSGTDPQEGSALARAILQYLIKKHIPCFVA
                     THFSELKTFAHVTPGAINASMEFDLKTLRPTYRLSIGLPGRSNALLIAEKLGLPAEIL
                     EDARETIDPEVQDTGALLDEIHRQQEAARKARSQADRLRSLAENDERKLRKQLEEIEA
                     EKEKILSEAHSETEEEIKELKTELAKLRKAYAAAKIPLGELKPLEEGLKESERAAQSI
                     ENHIEKKSKLKTYHAAPLKIGEKVRVRSLGTNSLATITTIDGDEAEILLGALRMRVPI
                     KDLIRKSDDTGNNDENTGGPTGKTESPVKMEERKGSGEIFHPSPGVEIDLRGKTAEEM
                     IDLLEKYLDEAVLAGLPYVRIIHGKGTGKLRQAARQFLLDSPYVKYIENGKDNEGGEG
                     VTVAHLKV"
     gene            2827..3579
                     /locus_tag="PKV59_02235"
     CDS             2827..3579
                     /locus_tag="PKV59_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45601.1"
                     /translation="MTGCDQVNIYSLTEPTETMTFQNQKTLVPTAIDSPTVTVQWFPA
                     TATPRPLSTPTAFPTKDMKPDVGEVLLEDVFLNENFWQTFRSDQGNAVISNNELTLAI
                     QFSAGSIASFLDYHPKTDYYLSVDVTLPICSDSSDYYGVFFRYGDKDNYDRFLINCQG
                     QTRVEQRYGGKMLPLADWSVNGQIRSGGPQKFTLGMRIKGETLRFFINDVFLYETKDS
                     LLKSGAIGLLANSQGVSPISVSFSNFKIYDLR"
     gene            complement(3583..4098)
                     /locus_tag="PKV59_02240"
     CDS             complement(3583..4098)
                     /locus_tag="PKV59_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45602.1"
                     /translation="MTEQNNEIPGNETQSEEIRAQKRLRNALFVTLGGYLILVLGAKP
                     SIFGLDRSPVIGFVQTATFIIGLGIICLGGRMSLRSFWKGKKISLTADFGLRVVATGY
                     VIAIFTAMADVFGFGSHPLPGVPFFGPLQARGVILGEAIIAVGLIMMFSPVDETEEKS
                     KDRQSNSEKSA"
     gene            complement(4095..5186)
                     /locus_tag="PKV59_02245"
     CDS             complement(4095..5186)
                     /locus_tag="PKV59_02245"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQP45603.1"
                     /translation="MNFIIDGHEDLAFEALANQRDTTRSVAEIREKEPQNTENQVTLG
                     WPEMQAGKVGIIFATIFIEPPAHQTPPKRTGVNYGTPDEFHQSVRKQLDFYEQWEDSC
                     PDKFTIIRSLSDLDQTASRWQREVENPTGSTLPVGLVLLLEGAEGLRKEDDLDEYYDR
                     GLRIVGPVWEGGRWCGGTYSPDSMRFTPEGKRFLEILSSKGFILDVAHMNTFSADEAL
                     DLYEGTVIASHCNCRRLLKSPENQRHFDDETIRKLIDRDGVMGVIPFNNFLDSGWKNG
                     MPRNRVTLDLLAQHIDHICQLAGNADHAAIGSDLDGGFGYPNIPEEMNDIQDLQKLEQ
                     ILQQKGYTPEDIMKIFNGNWIRVLKRGLP"
     gene            complement(5215..5769)
                     /locus_tag="PKV59_02250"
     CDS             complement(5215..5769)
                     /locus_tag="PKV59_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HQP45604.1"
                     /translation="MKNQTFYVLALFDEKTNQIFTELTENLKKNNIDFLEELPHITLG
                     GYTEVPESEITSWVKEFSETHCPLKINFNHIGLFPQSICFIGSKVNEELLRFHREFHA
                     NFDSCFTEIGYNFTLCSENWVPHMTIITGNAENIFKGIPILFSAFQPFIGTITELAIY
                     QFDPFLELRRFPLKHTDFQTDKKR"
     gene            complement(5981..>6260)
                     /locus_tag="PKV59_02255"
     CDS             complement(5981..>6260)
                     /locus_tag="PKV59_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQP45605.1"
                     /translation="LTKFGAFAKLDDNIEGLIHISEISEKRIDHAKEVLHEGEKITLR
                     VIKIDPANHRIGLSVRRVDSMAYADMDWESLLDSDLEAVEKSTEDEEK"
BASE COUNT         1737 a         1372 c         1425 g         1726 t
ORIGIN      
        1 atgtggtgca tcttgcccgt tattctccgc gaaaaggcac tttatcggag aggacgatgc
       61 tggatgatgt ctccgatgaa gaaaaatggc ggcgttttcg gaccgttgag acgttgcagg
      121 aacggattgc aaccgagctt cacgcggcct tggaagggaa aattcttcct gtgctgtttg
      181 aagaaaaaca aaaaggccgt tggatgggaa gaacagagca tatggacttg gtctatgtcg
      241 aaagcgaatc ggatttgaca ggtctgattt taccggtgaa aatgaattgg acgggaccct
      301 ggacgatgat cggtgaactc gtatagcaat tgataagtgc ataaaatgga caaaaaatca
      361 ctcaagatgc tggaatatcc ggaagtcatt cgaagacttg cgcaatatgc aagttttaat
      421 gtttcggaac aaatggcaca aaaccttcat cccgaaacgg attcttcgat cattcgctat
      481 aagctcagcc tgacaaccga agctcgaaaa ttgttaagtg tcaacgacac ttttcgcctc
      541 ggcggttgca gtgatttacg tccgtacttg gaagtcgccc ggaagcaggg aactttggaa
      601 gcgaaagctt ttgtggaaat tgcttatacg ctttcggtcg ccagagatct ttatcgttcc
      661 ctgtcccatc atgaagcgga atatcccaat ttagctgcga tcgcagaaaa cttgattcct
      721 ccggccggtt taatcgaacg gattaatttc acggtcaatg atcggggaga agtattagac
      781 agcgcttcgg agaaattgac gtcgataagg cgtgagatcc gcctttctta taaccgattg
      841 ttgggcagac ttgagaaaat ggtcaaggaa tcccgctatc aaccgatgct tcaggaaccg
      901 atcattaccc aacgaaacgg gcgatatgtc attcctctta aagccgaata taaagggcaa
      961 ctgaaatcga ttattcatga tcaatcctca agcggagcca ccttgtttgt ggaaccgctg
     1021 gcgacagtcg aattgaataa tgtttatcag gcattacaac tggatgaacg aaatgaaatc
     1081 atccggattt tgcagaattt gaccgctttt gttggtgact atgccgatca gatcgaacaa
     1141 tcagttgagg cgttggcgga aattgatttt gccatgatgt gtgccaaata tgccgatgat
     1201 ttgcgggcgg cggaaccgtt attggttgat ccctataaaa aaacagggaa tccggtcatt
     1261 aaactctatc aggcacggca tccgctttta ccggtagaaa ccgcggttcc gattgacgtt
     1321 gtaccggacg aagaaacgtt tgcggttgtc ataacgggac cgaataccgg cggtaagacc
     1381 gtatccctca aaacaatcgg gctgctgatc ctgatggcac aatccggttt gcatattccc
     1441 gtccgatccg gttcggaatt ctcggttttc aagaatgtct ttgcggatat cggagatgag
     1501 caatcgatcg aacaatcgct ctccactttt tcggggcata ttaccaatat tattcgaatt
     1561 ttgaaagaag ccgattccaa aacattggtt ttgctggatg aactggggtc gggaaccgat
     1621 cctcaggaag gttcggcttt agcgcgggcg atcctgcagt atctgataaa aaagcatatt
     1681 ccgtgctttg tcgcgactca tttttccgaa ctcaagactt ttgctcatgt gactccgggg
     1741 gcgattaacg catcgatgga atttgatctc aaaacattaa ggcctaccta tcgactcagt
     1801 atcggacttc ccggacgttc aaacgcttta ttaatcgccg agaagttggg attgcctgcc
     1861 gaaattttag aggatgctcg tgaaacgatc gatcccgaag ttcaggatac cggagcgttg
     1921 ttggacgaaa ttcatcgtca acaggaggcg gctcgaaaag cacgatcgca agcggatcgg
     1981 ttacgcagtt tagcagaaaa tgatgaacgc aaattgcgaa aacagttgga agagatcgaa
     2041 gcagaaaaag aaaaaatcct aagcgaagca cacagcgaaa ccgaagagga aattaaagaa
     2101 ctaaaaacgg agcttgccaa attgcgcaaa gcttatgcgg cagccaaaat acctctcggc
     2161 gagttgaaac cgcttgaaga agggttaaaa gaaagtgaaa gagccgctca atccatcgag
     2221 aaccatatcg aaaagaaaag caaattgaag acttaccatg cggctccgct gaagatcggt
     2281 gaaaaagtcc gggttcgttc gttaggaacg aattccttag ctacgatcac aacaatagac
     2341 ggagacgaag ccgaaattct gttgggagcg ctgcgaatgc gggttccgat aaaagatttg
     2401 attcggaaat ccgatgatac ggggaataac gatgaaaata ccggaggacc gaccggaaag
     2461 acggaatccc cggttaaaat ggaagagcga aaaggaagcg gtgaaatttt ccatccctct
     2521 ccgggtgtgg aaatagatct ccgcggaaaa accgccgagg aaatgatcga tctattggaa
     2581 aaatatctgg atgaggctgt tttagccgga cttccttatg ttcgaattat ccacggaaag
     2641 gggacgggaa aattgcggca agctgcccgg caatttctgt tggattctcc ttatgtaaaa
     2701 tatatcgaga acggaaaaga taatgaaggc ggagaaggcg ttacggttgc gcacctgaaa
     2761 gtgtgatcga tgatgatgcg aaggttgagt ttcaaattgc tcttttggat gatgctcttc
     2821 tctgtcatga caggttgtga tcaggtcaat atttattcct taacggaacc gaccgaaacg
     2881 atgacctttc aaaatcagaa aacgctggtt ccgacggcaa tcgattctcc gacggtgact
     2941 gtccaatggt tccccgcaac tgccacaccc cgcccgctca gtacaccgac cgccttcccc
     3001 acaaaggata tgaaacccga tgtcggggaa gttttactgg aagatgtatt cctcaatgaa
     3061 aacttttggc aaacctttcg atccgatcag ggaaatgccg tcatttcgaa caatgaattg
     3121 acacttgcga ttcaattctc ggccggttcg atcgcttcct ttttagacta tcatcccaag
     3181 acggattatt atttgagtgt cgacgtgact ttgccgattt gttctgacag ttcggattat
     3241 tacggagtat tcttccgtta tggcgataaa gataattatg atcgctttct gattaattgt
     3301 cagggacaga cacgggtgga acaaagatac ggcggaaaga tgttgccttt ggcggattgg
     3361 agcgtcaacg gacaaattcg ctctggcgga ccgcaaaaat ttactttagg catgcgaatc
     3421 aaaggtgaaa cattaagatt ttttataaat gatgtctttt tgtacgagac gaaagactcc
     3481 cttttaaaaa gcggtgctat cggcttattg gctaattccc aaggagtttc tccgatcagc
     3541 gtaagttttt caaattttaa gatctatgat ttacggtaaa tactaagcgc ttttttccga
     3601 atttgattgc cggtcttttg atttttcttc tgtttcatcg acgggagaaa acatcatgat
     3661 caacccgact gcgataatcg cttctcccaa aatgacgccg cgggcttgca acggaccgaa
     3721 gaacggtact cccggtagtg ggtgcgaacc gaaaccgaac acatcagcca ttgcggtaaa
     3781 tattgcgatc acatatccgg tcgctaccac gcgcagccca aaatcggctg tcaatgaaat
     3841 ctttttgccc ttccaaaagc ttcgtaagct catccggccg ccgagacaga taatcccgag
     3901 tccgataata aaagtggctg tttgcacaaa tccgatgacc ggactgcgat ccaatccgaa
     3961 aatggacggt ttggcgccca gaacaaggat caggtaaccg cctaaagtta caaaaagggc
     4021 gtttcgcagc cttttttgcg ctctgatttc ttcgctttgt gtctcatttc ccggtatctc
     4081 gttgttctgt tctgtcatgg taaacctctt tttaaaactc tgatccagtt cccgttgaat
     4141 atcttcatta tatcctccgg tgtatatcct ttttgctgca aaatctgttc caatttctgc
     4201 aaatcttgaa tatcattcat ctcttccgga atattgggat agccgaagcc gccatccaaa
     4261 tcagagccga ttgccgcatg atccgcattc cccgccaatt ggcagatatg atcaatatgt
     4321 tgtgctaaaa gatccagcgt cacgcgattc cgtggcatgc catttttcca accgctatcc
     4381 agaaaattgt taaacgggat cacacccatt accccatcgc gatcgattaa ttttcgaatg
     4441 gtttcgtcat caaaatggcg ctgattttcc ggtgatttca gtaatcggcg acaattacaa
     4501 tggctggcga tcacggttcc ttcataaaga tcaagggctt catcggcact gaaagtgttc
     4561 atatgagcga catccaaaat aaatcccttg gatgaaagaa tttcaaggaa cctttttcct
     4621 tccggagtaa aacgcatcga atcgggagaa taagttccgc cgcaccaacg gccgccttcc
     4681 cacaccggac cgacgattct caaaccccga tcataatatt catcgagatc gtcttctttg
     4741 cgcaaacctt ccgccccttc cagtaaaaga accaacccga ccggtaacgt cgagccggtc
     4801 ggattttcaa cttctcgctg ccaacgggaa gctgtttgat caagatctga aagagatcgg
     4861 atgatcgtga atttatcggg acaggaatct tcccattgtt cataaaaatc gagttgtttc
     4921 cgaacggatt gatgaaattc atccggtgtg ccataattga ctcccgtccg tttgggaggc
     4981 gtttgatgtg caggcggttc gatgaagatc gtggcaaaaa taatccctac tttccccgct
     5041 tgcatttcgg gccagccgag cgtcacctga ttttcggtat tttgcggttc tttttctctg
     5101 atttcagcta cggaacgcgt tgtatcccgc tgatttgcca atgcttcaaa tgctaaatct
     5161 tcatgaccgt caataataaa gttcataaat tttcctttgt tttgcccgaa agtttcatct
     5221 ttttttgtct gtttggaaat cagtgtgttt taacgggaac cttcgaagtt cgagaaaagg
     5281 atcgaattga tagattgcca gttctgtgat tgtcccgata aacggctgaa aagcggaaaa
     5341 caaaatcggg attcctttga agatattctc cgcatttccg gtgattatcg tcatgtgcgg
     5401 gacccaattc tctgaacaca aagtgaagtt atagccaatc tcggtgaaac aactgtcaaa
     5461 attggcatga aattctcggt gaaaacgtaa caactcctcg ttcacctttg atccgataaa
     5521 acagatactt tgggggaata atccgatatg attgaaattt attttcaatg ggcagtgagt
     5581 ttcagaaaat tccttaaccc aagaggtaat ttcgctttcg ggaacttccg tgtatccgcc
     5641 taatgttatg tggggcagtt cctcaagaaa gtcaatgtta ttttttttca ggttttctgt
     5701 taattcggtg aaaatttgat ttgttttttc atcaaataac gcgaggacat aaaaagtctg
     5761 atttttcatt aaatttcaca aacccgctga aaggaatcct tttcggttct ctcataaaat
     5821 ttactaaaga taattttacc gttttttgat tgttgcattt tattaaagtt ttacaacttt
     5881 ttccattcat tctatgcgat tcatccgatt tatataaaaa gttaaataaa aaggctgtcc
     5941 cgttatcttt gagacagcct gtattgttca gcttttcggc ttatttttct tcgtcttcgg
     6001 ttgatttctc gaccgcttca agatcactgt ccaatagtga ttcccaatcc atatctgcat
     6061 atgccatgga atctacccgt cgaacggata aaccgatccg atgattggcc gggtcaattt
     6121 tgatcacgcg taaagtgatt ttctcacctt catgcagcac ttctttggcg tggtcaatcc
     6181 gtttttcact gatttccgaa atatggatta gaccttcgat attgtcatcc aattttgcaa
     6241 aggcaccgaa ttttgtcaat
//
LOCUS       DAOMIV010000055         6115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_13010_length_6115_cov_4.542739, whole genome shotgun sequence.
ACCESSION   DAOMIV010000055 DAOMIV010000000
VERSION     DAOMIV010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13010_length_6115_cov_4.542739"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PKV59_02260"
     CDS             <1..981
                     /locus_tag="PKV59_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HQP45606.1"
                     /translation="TAVPVEVDIRTYTIDAGWAEKFIQSRYAEKEGRWINRKSGNRLA
                     GIIPVNEFGLCADIPAVNALAKRYGLKVFEDSACALGAKIGETHEGCFGNPSAVSFHP
                     RKSITTGEGGMILTSDVELMEHVRKLRSHAASLSEIQRHQNKGYLLPDYDELGYNYRM
                     TDIQAGIGLAQMRKIELIMNRRKAIASRYDEFLPQVVPFLKTPFVPEGYTHIYQTYAC
                     MIDTERLGMKSIEEGNRFRNELMDKLEQNGIATRQGTHAVHSLGYYQMKYGFRNMDLP
                     VAYACDRLSIALPLYVELNEEDQDYVIDKLAELGKELMNSLKSQTRSVEK"
     gene            990..1961
                     /locus_tag="PKV59_02265"
     CDS             990..1961
                     /locus_tag="PKV59_02265"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQP45607.1"
                     /translation="MPQIKGKNVLITGGAGFIGSHLTDALLKNQAGKVVIVDNFFLGK
                     MENLDFAQKNYNNLVIYRDDARDLGTMKAIIKKESVEVVFNLATIALNYSFFNPFGAY
                     RVNVDIAEVLLHLLQDGAYQTLIHSSSSEAYGTAVYSPMDENHPMNPTTPYAAGKASA
                     DLMIQSFYNVLHLDVAIIRPFNNFGPRQNDLALAAIIPQNARRILNGEKPVLEGTGEQ
                     TRDFIYVEDTARAFIMAYESEMTRGRVINLGSGKEISMKAVIDAICKYYGYNGGIEYR
                     PERPADVKRLCAGSELARELIGFTPQTDFETGLRKTLDWYQEKYTNL"
     gene            1958..2626
                     /locus_tag="PKV59_02270"
     CDS             1958..2626
                     /locus_tag="PKV59_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQP45608.1"
                     /translation="MILCVFSDIHGNRLYFDFLADVWKGMDIDRFFFLGDAIGYFPDG
                     NEVLDRLKEFNPVCVKGNHEAMLLGLLPLSDEKDEVYHLKEQKANLTEENLSFLKRWP
                     DTWEKDINGLRIHFVHGTISDPLTGTGYENSEFANFDQIGLDVLFIGQTHRPWIRRNK
                     HTLIVNVGSVGLPRDYGNQPSFVLFDTKTKAVSIWRVEIDPGPILDHPEGIHPSVLDV
                     LKRK"
     gene            2630..3676
                     /locus_tag="PKV59_02275"
     CDS             2630..3676
                     /locus_tag="PKV59_02275"
                     /inference="COORDINATES: protein motif:HMM:NF009402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQP45609.1"
                     /translation="MTNKIRVMVTGVGGGGNGEQLLKALKLAETPYHLIGADITPLSK
                     GLYMADERVILPPAVDPDYISVLTDTCKKNRVQALFTGSEPELKRISGFQDEFRAEGI
                     FLPMNSKSVIDLCLDKSKTMSFLKENGFYFPQTWTIRNSEDLQSVDLFPLVLKPSVGG
                     GGSANSFIAQDKKELELFGCWMLNIYSEFIVQQYIGDADSEYTAGVLCDMEGNLINSI
                     AVKRNILSALSNRLKIRSRYTGEMLVLSNGITQGEIGRFPEVTSQCEEIALKLGARSA
                     INIQLRFVDGKAYVFEINPRYSGTSSFRAMVGYNEPDLMIRKYLLNEEIEPHFAYKEG
                     TILRGLDEIFLEKR"
     gene            3673..4395
                     /locus_tag="PKV59_02280"
     CDS             3673..4395
                     /locus_tag="PKV59_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45610.1"
                     /translation="MTGDLSKTIYLTMDTDWAGDDVLSDSLTLIDELQVPVTIFITHE
                     TKLLETIRDHPLIRLGIHPNFYPLLQNHADQDYLSVIGEIKKIVPEAVSARSHGLIDS
                     TAILEAFKAQGITRDLNLFIPFSSGIELKPFRHFCGLLRIPYFYEDDAYCSENAGETA
                     SAHILSSGNGLKVFNFHPIHLFLNTENMNRYLDAKPFQREYSQLIKYVNRDRQIGARA
                     FLKQVVECGRENRFTFDHVENL"
     gene            4392..5306
                     /locus_tag="PKV59_02285"
     CDS             4392..5306
                     /locus_tag="PKV59_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45611.1"
                     /translation="MKLSEIPMADWQVIRDGEFLSLGLCGARPSVPILTFAGSEKYLK
                     TAVRNPDVSCILCPPELAASDLIRTSGKGICTCENIRVGFFKFHNELAKGDISDGYLR
                     GVFPVKIGRNAAISDDAILDSENIIIGDNAVIEPFVRIHANVQIGENSIIRSGTILGG
                     TGLEFIRTDDGGILPVTHCGGLVIGKNVEIQYNCNVSRSLFPWDDTVIGDDTKIESLV
                     HVAHGVKIGQRNLIAACACIGGSAELGNDIWVGPNATISSEVKVDDHSRISLGAVVAG
                     NVARGQTVTGNFAMNHEQFMQDQLRKML"
     gene            complement(5334..5645)
                     /locus_tag="PKV59_02290"
     CDS             complement(5334..5645)
                     /locus_tag="PKV59_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HQP45612.1"
                     /translation="MSATFIRRQEDFICEHCGKQVKGNGYTNHCPYCLYSKHVDINPG
                     DRASACGGLMEPIRAELRKNEVILTHRCLKCGCLKVNKTAPEDNFDEILRIMQNSQNT
                     K"
     gene            complement(5657..>6115)
                     /locus_tag="PKV59_02295"
     CDS             complement(5657..>6115)
                     /locus_tag="PKV59_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HQP45613.1"
                     /translation="DGGREKTATVLYGSMYGNTRFFAESVIQAVAQKGLPVEVFDINS
                     SHISYILPSLWKNQGVIICAPTYEGSIFPYMMNVLSMAKIKKVMNRSAAYFGSYAWGS
                     MAKKQFEDYCTEQKWDIVENRQFFGKPKSADFEMIPDFAERFVKAVKEAV"
BASE COUNT         1642 a         1258 c         1531 g         1684 t
ORIGIN      
        1 actgccgtgc cggtcgaagt ggatattcga acctatacca tcgatgcagg ttgggcggag
       61 aaatttattc aatcccgata tgcagaaaag gaaggtcgtt ggatcaatcg gaaaagcggg
      121 aatcgtttgg cgggaattat accggtcaat gagttcggac tttgtgcgga tattccggcg
      181 gtcaatgcac tggcaaaacg ctacggctta aaagtatttg aagacagcgc ctgtgccttg
      241 ggtgcgaaga tcggagaaac ccatgaggga tgcttcggga atccctcggc ggtcagtttt
      301 catccgcgaa agtcgatcac caccggtgaa ggggggatga tattaacttc cgatgtcgag
      361 ttgatggagc atgtgcgaaa acttcgttct catgccgcat cgctctccga gattcaacgc
      421 catcaaaata agggatattt actgccggat tatgatgagc tgggctataa ctatcggatg
      481 accgacattc aggcggggat cggcttagcc caaatgcgca aaatcgaatt gattatgaat
      541 cgccgaaagg caattgcctc ccgctatgac gaattccttc cgcaggttgt tccttttttg
      601 aaaacaccgt ttgtaccgga aggatatacc catatttatc aaacctatgc ctgcatgatc
      661 gatactgaac gactgggaat gaaaagcatc gaagaaggaa accgcttccg aaacgagctg
      721 atggacaaac tggaacagaa cggcattgcg acccgacaag ggacgcatgc cgtgcactcg
      781 ttgggctact atcaaatgaa atacggattt cggaatatgg atttgccggt tgcatatgcc
      841 tgcgatcgct taagtatcgc attgccgtta tatgtcgaac tgaatgaaga agatcaggat
      901 tatgtgattg acaaattggc ggaactggga aaagaactca tgaattcgct gaaaagccaa
      961 acccggtccg tcgagaaata gaggaatcga tgccgcaaat caaaggaaaa aatgttttga
     1021 ttaccggagg cgccggcttt atcggcagcc acttgacgga tgcattattg aaaaatcaag
     1081 caggaaaagt agtcattgtt gataattttt tcttgggaaa aatggaaaat ctcgattttg
     1141 cacagaaaaa ttacaacaat ctggtcattt atcgcgatga tgcccgtgat ttggggacta
     1201 tgaaggcgat catcaaaaag gaatccgtcg aagtagtctt caatttagcc accattgcgc
     1261 tgaattattc ttttttcaat cctttcggcg cttatcgggt gaatgtggat atcgcggagg
     1321 ttttactgca ccttttacag gacggagctt accaaacact cattcattcg tcttcttcgg
     1381 aagcttacgg gacggctgtc tattctccga tggatgaaaa tcatccgatg aatccgacca
     1441 cgccgtacgc ggccggtaaa gcctcggcgg atttgatgat tcagtccttc tataatgtgc
     1501 ttcatttgga tgtcgcgatt atccgaccgt ttaataactt cggtcccagg caaaatgatc
     1561 tggcattggc ggcgattatc cctcagaatg ccagaaggat attgaacggg gaaaagccgg
     1621 tccttgaagg aacgggggaa cagacacggg atttcattta tgtggaagat accgcgagag
     1681 cctttattat ggcttatgaa agcgagatga cacgcggcag agtgatcaat ctcggttccg
     1741 gaaaagaaat ttccatgaaa gccgtgatcg atgcaatttg taagtactat ggttataacg
     1801 gcggtatcga atatcgtccg gaacggccgg cggatgtgaa gagactgtgt gcgggttccg
     1861 aactggctcg tgaattaatc gggtttacac cgcagacgga ttttgaaacc ggcttgcgca
     1921 aaacgttgga ttggtaccag gagaaatata caaacttatg atactgtgcg ttttttcaga
     1981 tattcatggt aatcgtcttt attttgattt tctggcggac gtttggaaag gaatggacat
     2041 cgatcgtttc ttttttttgg gagatgcgat cggatatttt ccggatggga atgaggtgtt
     2101 ggatcggctg aaggagttca atcccgtttg tgtaaaaggc aatcatgaag ctatgctgtt
     2161 gggattatta ccattatcgg atgaaaaaga tgaggtttat catctgaagg aacaaaaagc
     2221 gaatcttacg gaagaaaacc tctcctttct caagcgatgg ccggatactt gggagaaaga
     2281 catcaacgga ttgcgaattc attttgtaca tggcacgatt tccgatccgc tgacagggac
     2341 cggatatgaa aattcggaat ttgcgaattt tgatcaaatc gggttggatg ttcttttcat
     2401 cggacaaact catcgtccct ggatacggcg aaataaacat accttgattg tcaatgtcgg
     2461 ttcagttggc ttgccgcggg attacgggaa tcaaccttct tttgtcttgt ttgacacgaa
     2521 gacgaaggcg gtctcgatct ggcgggttga gatcgatccc ggtcccattt tggatcatcc
     2581 ggaaggaatt catccttcgg tattggatgt tttaaaaagg aaataacgcg tgacgaataa
     2641 aatcagagta atggttacgg gtgtcggcgg tggcggtaac ggcgaacaat tattgaaagc
     2701 tttaaagctg gctgagacac cttatcatct gatcggtgcg gatataacgc ctttatccaa
     2761 agggttgtat atggcggatg aacgggttat tcttccgccg gctgtcgatc ccgattatat
     2821 ttccgttctg acggatacct gtaaaaaaaa ccgtgttcag gcattgttta ccggatcgga
     2881 gccggagtta aaacggatca gcggatttca ggatgaattt cgagctgagg gtatcttttt
     2941 gccgatgaat tcgaaatccg taatcgatct ttgtctggat aaatcaaaga cgatgtcatt
     3001 cctgaaagaa aacggttttt attttccgca aacatggaca attcgaaatt cggaggattt
     3061 acaatccgtg gatctattcc cgttggtttt gaaaccttcg gtgggaggag gcggatcggc
     3121 taacagtttt atcgcacagg acaaaaagga attggagctt ttcggttgct ggatgttaaa
     3181 catttattcg gaattcatag ttcagcaata tatcggtgat gccgacagcg agtatacggc
     3241 cggtgtgctt tgtgatatgg aagggaatct gatcaattcg attgccgtaa aaaggaatat
     3301 tttatccgca ttgagcaacc ggctgaaaat ccgcagccgc tataccggag agatgctggt
     3361 gctcagtaat ggcattacac agggtgaaat cgggcgattc cccgaagtta cttctcagtg
     3421 cgaagaaatt gcgttgaaac tgggggcacg ttcggcgata aatattcaat tacgctttgt
     3481 agacggaaaa gcatatgttt ttgaaatcaa tccgcgatat tccggaacct catcttttcg
     3541 ggcgatggtc ggatacaatg aaccggatct gatgatccgg aaatatttat taaacgaaga
     3601 aatcgagcct catttcgctt ataaagaagg gacgatttta aggggtttgg atgaaatatt
     3661 tttggagaag cggtgacagg tgatctttcc aaaacgatct atctgaccat ggataccgat
     3721 tgggccggcg acgatgttct ttcagattca ctgactctga ttgacgaatt gcaagttccg
     3781 gtaacgattt ttatcactca tgagacgaaa ttattggaaa cgattcgtga ccatccgctc
     3841 attcgcttgg gaattcatcc gaatttttat cctttgttgc agaatcacgc tgatcaggat
     3901 tatttatccg tcatcggtga aatcaagaag attgtacctg aagcggtcag cgcacgttct
     3961 cacggattga tcgattcaac ggcgattttg gaggctttta aagcacaggg gataacccgt
     4021 gatctcaatt tgtttattcc cttcagttcc ggaatcgagc tgaaaccttt tcggcatttc
     4081 tgcggattgc tacgtattcc ctatttctat gaagatgatg catattgctc ggaaaatgca
     4141 ggggaaacgg catccgcaca tattttgtct tcggggaacg gtctaaaagt atttaatttt
     4201 catcccatcc atttattttt aaataccgag aatatgaatc gctatttgga tgccaaacct
     4261 tttcaaaggg aatattccca gctgataaaa tatgtcaacc gcgaccggca gatcggagca
     4321 cgagcttttc tgaagcaagt tgttgaatgc gggcgcgaga accgttttac tttcgatcat
     4381 gtggagaact tatgaaactc agcgaaattc cgatggctga ttggcaggta attcgggacg
     4441 gcgaattcct ttctttgggt ttgtgcgggg cccgaccttc cgttcccatt ctgacgtttg
     4501 ccggaagcga aaaatatctg aaaaccgcgg ttcgcaatcc cgatgtcagc tgtatccttt
     4561 gcccgccgga gttggcggca tccgatttga ttcggacaag cggaaaagga atatgtacct
     4621 gtgaaaatat tcgagtcggc ttttttaagt ttcacaatga attagctaaa ggagatattt
     4681 cggatggtta tctgcgagga gtatttcccg taaaaatcgg caggaatgct gctatttccg
     4741 atgatgcgat actggattcg gaaaatatca tcatcgggga caacgccgtc atcgaacctt
     4801 ttgtccggat tcatgcgaat gtgcaaatcg gtgaaaacag tattattcga tcggggacca
     4861 tcctcggcgg gaccgggttg gagttcatcc gaacggatga cggcgggatt ttaccggtga
     4921 ctcattgcgg cgggttagtg atcgggaaaa atgtcgaaat ccaatataac tgtaacgttt
     4981 cccgttctct gtttccgtgg gatgacacgg ttatcggtga tgatacgaag atcgagagtc
     5041 tggtgcatgt tgctcacggg gttaaaatag ggcagcggaa tctgatcgcc gcttgtgcgt
     5101 gtatcggcgg ttccgccgaa ttaggcaacg atatttgggt tggtccgaat gcgactatct
     5161 ccagtgaagt gaaagtggat gatcattccc gcatcagtct cggagcggta gtggccggca
     5221 atgtggcgcg agggcaaaca gtgaccggca attttgcgat gaatcacgaa caattcatgc
     5281 aagatcaact gcgaaagatg ttatagctct ttttatactt atccatatgg aatctatttt
     5341 gtgttctgtg aattttgcat aattcgcaaa atttcatcaa aattatcttc cggagctgtt
     5401 ttgttgactt tcaggcaacc gcacttcagg cagcgatgag tgagaatcac ttcattcttt
     5461 cgcaattctg cccggatggg ttccatcaac cccccgcaag ccgaggcgcg gtcccccggg
     5521 ttgatatcca catgtttact gtaaagacaa taagggcaat gatttgtata cccattgccc
     5581 ttgacctgct ttccgcaatg ttcacaaata aaatcttcct gccggcggat aaaagtggca
     5641 gacatgaatc acgaacctat accgcttctt ttacggcttt tacaaaccgt tcggcaaaat
     5701 ccggaatcat ttcgaaatcc gcggatttcg gtttcccgaa gaactgcctg ttctcaacaa
     5761 tatcccattt ttgttcggtg cagtaatctt caaattgttt tttcgccata ctcccccagg
     5821 catagctgcc gaaataggct gcacttcgat tcatcacttt cttgatctta gccatcgaca
     5881 gaacattcat catgtaagga aaaatcgatc cttcataggt cggtgcgcaa ataattactc
     5941 cctgattttt ccaaagagaa gggaggatat aactgatgtg ggacgagttg atatcgaata
     6001 cctcaaccgg cagtcctttt tgtgcgacag cttgaataac agactcggca aaaaatcggg
     6061 tattcccata catggagcca taaagaaccg tggctgtttt ttcgcgtccg ccgtc
//
LOCUS       DAOMIV010000056         6041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_13235_length_6041_cov_4.826428, whole genome shotgun sequence.
ACCESSION   DAOMIV010000056 DAOMIV010000000
VERSION     DAOMIV010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_6041_cov_4.826428"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1667)
                     /locus_tag="PKV59_02300"
     CDS             complement(<1..1667)
                     /locus_tag="PKV59_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45614.1"
                     /translation="MAENILSTQIMQLILNWKLEEATDLLEANQSELSVKDIFELRGL
                     NERYQGYLDNFHEAESIVEEDPDAAQYAVDQIPEDIRITYPGYESLLAKLNTVRDVIA
                     RKQAFERVDEAYNAIVIEFNSAKAAEALETAKQIYPDWDRVPELREKILKTSELQEKL
                     ERGLYLQTEVSALREKGGIASYQKAMSLINEYSSLGLESYGIALFDADHDREDLLKMI
                     TRAEGESWKNRLRPDGIPAETLRLEQSIRSLEDAESKNLRVLYNNNARLMILLTSELQ
                     QEPPDSERAIQINSRINELREKNQKIQTDIHDEVAKRANSYCRLAEKALDAGELSSAE
                     INIRLAKETGKPAAEYDEGDYLGEVSLPTETLDKILSLEERYADALSVRNEIAEKYRI
                     IRDEYNADDNITLNKLLSWVNQIDDYFARDSHTPGLAQFRDELHKRYDSVKSYVFEKG
                     LAEIDRAINKGDPAAAKEKLDQLNSLNLDTDEKRTLRNRQTSINQLEDANNQVENLLK
                     KADEILQNALNPISSDRDSVREYEDIFREIESVYSQNAVKEPQSLIER"
     gene            complement(1696..4794)
                     /locus_tag="PKV59_02305"
     CDS             complement(1696..4794)
                     /locus_tag="PKV59_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45615.1"
                     /translation="MKQDNLEKIITEVNSVRVRDKVIPLVYTGVKNSGGFTSAFEFSN
                     QETEEKYFLKVSEIAEGSKDETSTLTKVNLLFKNEILSEPDAAKRLNYMPVPDVLTDD
                     IDKFNVEFMRMLDRNRFTVQLQTAGKGIPCEKQLPDSLKDRVAIALDFAKILRTCAKN
                     KIAYVDIKPLEHLFWSKTEDKISVTLIDWGNSRQNASSFLLLDDIRKFCLFLPELIYG
                     RKMLEVINQGKYEYPIQKENNPILIRLLGQLSFNTDNPPLNQKYALLIGDMLAGNLTE
                     VRVQNKCVEIWDDILRALNEAVDSLMTETKTTVLSYESLKKEAENFIQKDPESFMDKD
                     FRKAMDPRLISLASYKGWLLPVIRTVQFWYGKVDLIPQQGFDVCIRFVLNDQTTALNS
                     EFQKLAKLISEKISRTKSCPELIPILQDHLKQIQDVIYVWDFLAQSESGQISPEMFQA
                     AFTTSNLRVVDPFLADAYRKQARHISENSVPVSSQNNKVIKPDSLSEETENSKMENLE
                     DHTTEIKKRTQPLLDFFSRANGFSELRNIGFFADLNELLSGTKGEDSLLVEKELEPVF
                     LKMLDETEDWINKVGPDRFVVPDDQLNSLDWSAVLPQAVADIRVIVKNNPIQIGEFIR
                     SHMFNLQNDLARASSASIELNQKLPVRDLLPRIKALRKKLDTENMEQFKALIENGDFE
                     AAKEIIDLHYIEYPAFYERLRTEMSRQQDEGEDKKALALINSLLNDLSTASGNIETGK
                     YLRNQNNIPYVHQKISSFRQKNMQLYDIQDELARTKNLANESRKASKDLQRLTTLNLV
                     LLVVVIVLSVTLLTVVLIKNFNLDRNVARLEANLSNFQKTYIAAEQATPIPTQPPLPT
                     ATIAITPTEMPIAVAPLITDTEAVPAAQLTEDALLSPADLHLKSLIGKDVVFNLDGNV
                     DLYGDELMTPEKKLGVVINYVQNVPGKLIGYTDKMVNLEIPFNIGKSQISASTMQNVA
                     RQTNIRQYSETPSSTPPVFISQTEIVLTAPATNCGATAESFCNGTISVWIPREKIETS
                     IK"
     gene            complement(4881..5675)
                     /locus_tag="PKV59_02310"
     CDS             complement(4881..5675)
                     /locus_tag="PKV59_02310"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQP45616.1"
                     /translation="MYKKHLVLSDIHENGESLFRFMRKAEAMESGFDDIWLLGDLFGH
                     SNQSNGNAKFDLGFLDALDRLADYPISAVFGNWEYWLLHPERDNTSEAQAKCKAELEE
                     RRQIITERKIRLFDKIMKKEVVCLPEENPQFTVFHGCSFPCHDGQVYHAEPCESYLNP
                     KDLNIVTGGLFENRENLKTQHFLFGHTHIPGYFVYSVSTFVNIWRQFTPSVPEQKIEY
                     GNGIQRFGINPGSAGVEGKDFPRTALILDTEEQTFQFLADREPLYS"
     gene            complement(5668..>6041)
                     /locus_tag="PKV59_02315"
     CDS             complement(5668..>6041)
                     /locus_tag="PKV59_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45617.1"
                     /translation="SPCAALSVPAAIRFMEANHWDQVRRACHLLLFETLREIGQITQL
                     PLIYPNDDAYAQLAVTEIPYLPDPLVLKNFLYDRYQVEVPIINWNGRTFVRISVQGYN
                     TKMDVEALVEGLGDFLAHKHV"
BASE COUNT         1541 a         1339 c         1296 g         1865 t
ORIGIN      
        1 cgttcgatta agctttgcgg ttctttaacc gcattttgag aatagacgga ttcgatttcc
       61 cgaaagatgt cttcatattc ccgaaccgaa tctctgtcgc ttgaaatagg attaagcgca
      121 ttttgtaaaa tttcatccgc ttttttcagc aaattttcga cttggttgtt tgcatcttcc
      181 agttgattga tacttgtttg gcgattccgt aaggtcctct tttcatccgt atccaaattg
      241 agggaattaa gctgatccag tttctctttt gccgcggccg gatcgccttt gttgattgca
      301 cgatcgattt ccgcgagtcc tttttcgaat acataacttt taacggaatc atatcgttta
      361 tgtaattcat cacggaattg agcgagtccg ggagtatgag agtctcgggc aaaataatcg
      421 tcaatctgat taacccagga cagtaattta ttcaacgtga tattgtcatc cgcgttatat
      481 tcgtcacgaa tgattcggta tttctcggcg atttcatttc gaacggataa agcatcggca
      541 tatcgttctt ccaacgaaag gattttatcc aacgtctcgg tcggaagact gacttcgccg
      601 agataatcgc cctcgtcata ttcggcggcc ggttttccgg tttcctttgc caaacggata
      661 ttaatttcgg cggagcttaa ttctcccgca tccagtgcct tttccgccag ccggcaatag
      721 gaatttgccc gttttgccac ttcatcatga atatcggttt gaatcttttg attcttttcc
      781 cgtaattcat tgatccgaga attgatctga attgcccgct cgctgtcggg aggttcctgt
      841 tgcagttcgc ttgtaagcag aatcattaac cgggcattgt tgttgtatag tacccgcaga
      901 ttcttacttt cggcatcttc aagggaacgg atggattgct caagacggag cgtttcggcc
      961 gggattccgt cagggcgcaa tcggtttttc caactttctc cttccgcccg cgtaatcatt
     1021 ttaagtaaat cttcccgatc gtgatccgca tcgaaaagcg caatcccgta tgactccaaa
     1081 ccgagtgaag aatattcatt aatcagggac attgccttct gataagaagc gatgcctcct
     1141 ttttctcgga gtgcgctgac ttcggtttga agataaagcc cacgctcgag cttttcctgc
     1201 aattcggaag ttttcaatat tttttcacgc agctcgggaa cccgatccca atccggatat
     1261 atctgtttgg cggtctcaag cgcttcggcg gctttggcgc tgttgaattc gatgacgatg
     1321 gcattataag cttcgtcaac tctttcgaat gcctgcttcc gcgcaataac gtcccggacg
     1381 gtgttcaatt tggcgagtaa tgattcatat cccggatagg taatgcgaat atcttccgga
     1441 atttgatcga cggcatattg ggcggcgtcc gggtcttctt caacaatgga ttcagcttca
     1501 tggaaattgt ccagatatcc ctggtagcgt tcattcaatc cccgcaattc gaagatatct
     1561 ttaacggaca gttccgactg atttgcttcg agtaaatcag tcgcttcctc taacttccag
     1621 ttgaggatca attgcataat ttgggttgag aggatatttt ccgccattct tcgcttttac
     1681 aggtgcatta ggatgttatt tgatcgatgt ctcgattttt tcacggggaa tccaaacgga
     1741 aatagtcccg ttacagaatg actccgccgt cgcaccgcag tttgtggcag gcgcggtcaa
     1801 aacaatttcg gtctgcgata taaaaacagg cggtgtactg gacggggttt ccgaatattg
     1861 acgaatgttt gtttggcgtg ccacattttg catggtgctg gcggatattt ggctctttcc
     1921 gatattaaac gggatttcga gattgaccat tttatccgta tatccgatca gtttcccggg
     1981 aacattttga acatagttga tgaccactcc cagttttttc tctggagtca ttaattcatc
     2041 cccataaaga tcgacattcc cgtcaagatt aaacacaaca tccttaccga ttaaagattt
     2101 caaatgtaaa tcggccgggc ttaacaatgc atcttccgtc aattgagcgg cgggaacagc
     2161 ctcggtatcg gtaatcaaag gagcgactgc gatcggcatt tctgtaggcg tgatcgctat
     2221 ggtggcagtg ggcagcggcg gttgagtcgg aatcggagtc gcttgttcgg cggcgatata
     2281 ggtcttttgg aagttggaca aattcgcttc caatcgggca acatttcgat ccaaattgaa
     2341 attttttatc aagaccactg tcaataaagt gacagataag acgatgacga caactaataa
     2401 caccaggttc aatgttgtca gccgttgtaa atcttttgag gctttacggg attcgtttgc
     2461 cagatttttt gtccgtgcca gctcatcctg aatatcgtaa agttgcatgt tcttttggcg
     2521 aaagcttgag attttttgat gaacataagg gatattattt tggtttcgta aatatttccc
     2581 ggtttcgatg ttaccactgg ctgtcgacag atcattcagc agggaattaa tcaaggccag
     2641 tgctttttta tcttcgcctt catcttgttg acgactcatt tctgtacgta atcgctcata
     2701 aaatgccgga tattcaatgt aatgcaaatc gattatttct ttggcagctt caaaatcgcc
     2761 gttctcgatc aaagctttga attgctccat attttcggta tccaattttt tccggagagc
     2821 tttgattctc ggcagcagat cacggaccgg cagtttttgg ttcaattcga tggaagcact
     2881 gctcgctctc gccagatcat tttgtagatt gaacatatga ctgcggatga attcgccgat
     2941 ttgtatcgga ttatttttta caattaccct tatgtcggca acggcttgcg gcaaaacagc
     3001 agaccagtcc aggctgttta attgatcgtc gggtaccaca aatcggtcgg ggccgacttt
     3061 attgatccaa tcctccgtct catctaacat ttttaagaag acaggttcca actctttttc
     3121 gaccaataac gaatcttccc cttttgttcc cgacaacaat tcattcaaat cggcaaaaaa
     3181 gccgatattt cttaattcgg aaaaaccgtt cgctcgggaa aaaaagtcca ataacggttg
     3241 agttcgtttc ttgatctcgg ttgtatgatc ttcgagattt tccattttag aattctccgt
     3301 ctcttcggac aaagaatccg gttttatgac tttgttgttt tgagaagaga ccgggacaga
     3361 attttctgaa atatgccgtg cttgcttgcg ataggcatcg gcaaggaacg gatcgactac
     3421 gcgtaaattg gaggttgtaa aagcagcctg gaacatctca ggtgaaatct gaccgctttc
     3481 tgattgagca aggaaatccc aaacatagat tacatcttga atctgcttga gatgatcttg
     3541 aagaatcgga atcagttcgg gacaactttt tgttcgggaa attttttcgg atatcagctt
     3601 cgccaatttt tggaattcgc tgtttaatgc ggttgtttgg tcatttaaga caaatcggat
     3661 gcagacatca aaaccttgct gaggaatgag atcgactttg ccataccaaa attgaacggt
     3721 tcgaatgacc ggcaacagcc aacctttata agaagcaagc gaaatgaggc gcggatccat
     3781 tgcctttctg aaatctttgt ccatgaagga ttcgggatct ttttggataa agttttccgc
     3841 ttctttttta agtgattcat aagaaagaac ggttgttttt gtctcggtca tgagagaatc
     3901 cacggcttca ttcaacgccc gtaaaatatc atcccaaatt tcaacgcact tgttttggac
     3961 gcgtacttcg gtcagatttc cggccaacat atcaccgatc agcagcgcat atttttggtt
     4021 cagaggagga ttatcggtat tgaaggaaag ttgtcccaac aaacgaatca ggatcggatt
     4081 attttctttt tggatcggat attcatattt tccttgattg attacctcca gcatttttcg
     4141 accgtaaatc aattcgggaa gaaaaaggca aaacttacga atatcatcca gtaacagaaa
     4201 gctcgaagca ttctgacggg agttccccca atcgatcaag gtgacggaaa ttttatcttc
     4261 cgttttcgac caaaacaaat gttccaacgg tttgatatcg acgtaggcaa ttttattctt
     4321 tgcgcaagtc cgaaggattt tcgcaaaatc gagagcgatt gctacccgat ctttcaatga
     4381 atccggcaat tgtttttcgc aggggattcc ttttccggcg gtttgaagtt gtacagtgaa
     4441 gcggttacga tccaacattc gcataaattc aacgttgaac ttatcgatgt cgtctgtcaa
     4501 aacgtcggga accggcatat agttcaaacg ctttgcggcg tccggttcgg ataatatttc
     4561 atttttgaat aataaattta ctttggttaa tgtcgaagtt tcatccttcg agccttcggc
     4621 gatttcggaa accttcaaaa agtatttttc ttcggtttcc tgatttgaaa actcgaatgc
     4681 ggaagtaaac ccacccgaat ttttcacacc ggtataaaca agcgggatga ctttatcccg
     4741 tacgcggact gaatttactt ccgttatgat tttttctaag ttatcttgtt tcatcgtatt
     4801 ctcctgaccg aacggctcac gcaggtcagc gctattttcc gagtccggtt gatttatcag
     4861 cggtgcggtt gcgaaatctt tcaggaataa agaggctccc ggtccgcaag aaattgaaag
     4921 gtttgctcct ccgtatccag aatcagtgcg gtgcgcggaa aatctttgcc ttcgactccg
     4981 gcactgcccg gattgattcc gaatcgttgt atgccgtttc cgtattctat tttttgttcc
     5041 gggacggagg gagtaaattg tcgccatatg ttgacaaacg ttgaaacgga atatacaaaa
     5101 tatcccggaa tatgggtatg tccgaacaag aaatgctgcg ttttcagatt ttctcggttt
     5161 tcaaaaagtc cgccggtcac aatgttcaaa tccttcggat ttaaataact ctcacagggc
     5221 tcggcatgat atacctgccc gtcatggcaa ggaaaactgc atccgtgaaa aacagtgaac
     5281 tgaggatttt cttccggcaa gcaaaccact tcttttttca ttattttatc aaaaagccgg
     5341 attttccttt ccgtaatgat ttgtcgcctt tcttctaatt ccgctttgca ctttgcctgt
     5401 gcttcgcttg tgttatctcg ttccggatgc aacaaccaat attcccagtt cccaaatacg
     5461 gcactgatcg gataatcggc taatcgatcc aaagcatcca aaaaaccaag atcaaatttc
     5521 gcgtttccat tcgattgatt tgaatgccca aacaaatctc ccagcagcca aatgtcatcg
     5581 aatccggatt ccatcgcttc ggcttttctc ataaaacgga aaagactctc cccgttttca
     5641 tgaatatccg acagtaccag atgtttttta tacatgttta tgagctaaaa aatctcctaa
     5701 tccctcaacc aaagcctcaa catccatttt agtattgtaa ccttgaaccg aaattcgaac
     5761 aaacgtgcgt ccgttccagt tgattatcgg gacttcaacc tgatatcgat cataaagaaa
     5821 gttcttcaga accaaaggat caggtaggta aggtatttct gtgaccgcca gttgggcata
     5881 agcatcgtca ttcgggtaaa ttaaaggtaa ttgggtgatc tgcccaattt ctcttaacgt
     5941 ttcaaacaat aacaaatgac aagctctccg aacttgatcc caatgattgg cttccatgaa
     6001 tcggatggca gccgggacgc ttaaggcggc gcaaggactg t
//
LOCUS       DAOMIV010000057         5970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_13476_length_5970_cov_3.823838, whole genome shotgun sequence.
ACCESSION   DAOMIV010000057 DAOMIV010000000
VERSION     DAOMIV010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13476_length_5970_cov_3.823838"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..>595
                     /locus_tag="PKV59_02320"
     CDS             271..>595
                     /locus_tag="PKV59_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQP45618.1"
                     /translation="MAKEQYVFESEGKIIRYRFPTHINDLIIPREESTCAEVFMVVLD
                     ENEAPPLHKHDDAEQIFYMIQGKGKLEIRKQQNDEPEYFEINVGQVIKIPPHTWHRAF
                     ALSKEG"
     assembly_gap    596..605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            814..2742
                     /locus_tag="PKV59_02325"
     CDS             814..2742
                     /locus_tag="PKV59_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45619.1"
                     /translation="MSRRISILGIILVFATMFLIAGAVSAADYHEAPMLADKVAAGEL
                     PPVAERLPEEPLVEQPLESIGVYGGTLRRGITGPNDHNSYVRWVYDSLVRFSQDGNST
                     YPHLIKSLEPNEDYTVWTFNMLKGAKWSDGSPFTSADIMFWFHDYYTNPDLAPAKLPW
                     LYNDDGSLVEVTAPDDYTVVFTFKDPITTVPMEVAAMDNRDNLVPMFLPSAYLKQFHA
                     KYTDVDKLNQMAKEQSFENWKQLFENKQAPFRNPERPVMAAWMSVTTINDPIYTLVRN
                     PYYPIVDTEGNQLPYIDQINFVTYQSTDALNMAVIQGELDMQDRHVVMQNYPALVEQA
                     EKTGKYEVRLWPTFGGVDAALSVNYYYDKDPDYMKLFQNHDFRVALSLGIDREEIIQS
                     AFLGVGEARQPVPRNTSPYYPGDEYAYKYTEFDQAKANEILDGLGMTKRDSQGFRLMP
                     DGSRCMIEIAYVPQFANWGDIAQMISEDWRGIGIDGVVVENERTNHFAKRDSNELMTE
                     IWNEDTTGFPFTGAPKFDPRTSPGICVATGARTWINSGGKEGYEPNADFKRIMEIIDS
                     AKRVSPDKQAELGKELFRIWAEGCYEIGIAGLTPLVQGVAVINVNLKNVPASNELGND
                     WPLRTPGNARTEQFYFAK"
     gene            2896..3879
                     /locus_tag="PKV59_02330"
     CDS             2896..3879
                     /locus_tag="PKV59_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45620.1"
                     /translation="MGKFLAKRLLLIPFLLLIFSIIIFALIQAPPGDFMTTYSAILAG
                     SGSSLDAEQLNAMRVSYGLDQPMHIQYIRWLGKLLHGDMGLSLLYMLPNKQLIGERLG
                     PTILLALFSFILSWLIAIPSGIYSATHKNSFGDYFFMVINYIGVAIPNFFLALLLMWF
                     FYSHYGLNLVGLFSPEFEMAPWSFAKFLDMLQHIWLPAIILALGGSARLTRVMRANLL
                     DELGKPYMITGRAKGMSEWALIMKYPVRLAINPLVSTIGNYIPQLFSGSLIVATVMNL
                     QNIGPLLLQALSMQDMYLSGSILIIYCLLGILGTILSDLLLAWVDPRIRLE"
     gene            3885..4043
                     /locus_tag="PKV59_02335"
     CDS             3885..4043
                     /locus_tag="PKV59_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45621.1"
                     /translation="MTENYSPKNNSNPTKNNPSESSVENQANIDLDALASTKSEEKVS
                     IASNGQLI"
     gene            4059..4130
                     /locus_tag="PKV59_02340"
                     /pseudo
     CDS             4059..4130
                     /locus_tag="PKV59_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024313.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4443..5081
                     /locus_tag="PKV59_02345"
     CDS             4443..5081
                     /locus_tag="PKV59_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45622.1"
                     /translation="MYATQTSLTVGLVSVLLSVFLGVLLGGISGYFGGMTDMVIQRLI
                     EILNSIPYVPIWMAMTAALPRQWSAEQRFFAITVILSLLGWTTLGREVRGKFMSIREE
                     DFILSAELIGCSKWRIIMKHMVPTFLSHIIATGTLSIPAMIVNETSLSFLGLGLRPPA
                     ISYGVMLQEAQNVQTMAKAPWLMLPGLVLVINVLMFNLIGDGLRDAADPYSK"
     gene            5090..>5970
                     /locus_tag="PKV59_02350"
     CDS             5090..>5970
                     /locus_tag="PKV59_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45623.1"
                     /translation="MENTKPLLSVRDLKVYFDLDEGTVKSVDGISFDAYEGKVVGIVG
                     ESGCGKSVTMKSILRIVEKPGRIVEGDILLQKRDNPDEYLNLVKMKENGKEIRAVRGG
                     EIALIPQEPMSAFSPIHTIGDQITEAIMLHNPVDKEEARRICIESLKSVGMSMPEKRI
                     DSYSWQLSGGLRQRAIIAMAIVCKPRLLIADEPTTAIDVTTQAQVLKLLKKLQMERNM
                     GILFITHDLGVIAQMAQYVVVMYLGRIMEKGPVDSIFHNPKHPYTQALLRSIPSIQKT
                     TKVKLPTISGSIPHPFN"
BASE COUNT         1736 a         1194 c         1340 g         1690 t
ORIGIN      
        1 tggatatgtt aaattacatg tttgacatcc actaccagga ccagcatcat ttccagtgca
       61 cccgcaatcc tcaggaaata atacaggcaa ttaggtactg tcacgtctat attaagaata
      121 taaaggtctt tttggataaa actaacagat tacccaaggc tgttataaaa aatgccctac
      181 gtgaaaagga attggaaaga tctgagtcat tgaataaaca ttaatatata taatcaattt
      241 cattataaat tcaacaaagg acgtgaaaaa atggcaaagg aacaatatgt ttttgaatca
      301 gaaggaaaaa ttattcgtta tcgatttccg actcatatca atgatttgat tatccccaga
      361 gaggagtcaa cctgcgccga agtatttatg gttgtcctgg atgaaaatga agctccccct
      421 ctccataaac atgatgatgc cgaacagatt ttttatatga ttcaagggaa aggcaaactt
      481 gaaataagaa agcaacagaa tgacgaaccg gaatatttcg aaatcaatgt cggacaggtt
      541 attaaaatcc cgcctcatac atggcatcgg gcttttgctt taagtaaaga aggcgnnnnn
      601 nnnnncctga gcattgattg tttcccaaat gggtttgatc cgtccgaacc gacctgggat
      661 gatcatgtta aaaacatggc aactgccaac ggatgggact tttccaaaat tcgtgaagac
      721 agacgctgag aaaaattcgg gcaggagttt gatcagatgt ctataaaaat ccggaatcgt
      781 attccggaat gaataaacca aataggagga aaaatgtcaa gaagaatatc aatattaggg
      841 atcatcttag tttttgcaac gatgttccta atcgccggtg ctgtttcggc tgccgattat
      901 catgaagcgc cgatgctggc tgataaagtc gcagccggcg aattgccccc ggttgcagaa
      961 cggctcccgg aagagccgct tgtcgaacaa ccattggaat cgatcggtgt ttacggtggt
     1021 acgcttcgac gcgggatcac cggaccaaat gaccacaaca gttatgttcg ttgggtctat
     1081 gactcactgg ttcggttcag tcaggacgga aactcaactt atccgcatct gatcaaatca
     1141 ttggaaccca atgaagacta cacagtctgg acatttaata tgttaaaggg cgcaaaatgg
     1201 tctgatgggt caccgtttac ttctgccgac atcatgttct ggtttcatga ttattatacg
     1261 aatccggatc tcgcacctgc caaactgccc tggctgtaca atgatgacgg ttcgttggtt
     1321 gaagtaaccg caccggatga ttatacggtt gtgtttacgt ttaaggatcc catcacaact
     1381 gtcccgatgg aagtggctgc aatggataac cgtgataatt tggttcccat gttcctgccg
     1441 tcagcttact taaaacaatt ccatgcaaaa tataccgatg tcgataaact caaccagatg
     1501 gcgaaagaac aatcttttga aaactggaaa cagctttttg agaataaaca agcgcctttc
     1561 cgcaatccgg aaagacctgt gatggcagca tggatgtcgg ttaccacgat taatgatccg
     1621 atttatacat tagttcgcaa cccgtattat ccgatcgttg acacggaggg aaatcaactc
     1681 ccttatatcg accaaatcaa ttttgtgact tatcaaagta cagatgctct aaacatggct
     1741 gtcatccaag gtgaattgga tatgcaagac cgccatgttg tgatgcagaa ttaccctgcc
     1801 ttggtcgaac aagctgaaaa aaccggaaaa tatgaagttc gcttatggcc gactttcggt
     1861 ggcgtcgatg ctgctttatc ggtcaattat tattatgaca aagatccgga ttatatgaag
     1921 ttattccaaa atcatgattt ccgcgttgca ttgtcactcg gtatcgatcg ggaagaaatc
     1981 attcaaagtg catttttggg agtcggggaa gcacgacaac cggtcccaag gaacacctca
     2041 ccgtattatc cgggagatga atatgcctat aagtacaccg aatttgatca ggcaaaagcc
     2101 aatgaaattc tggatggttt gggaatgaca aaacgggatt cccaaggatt cagattaatg
     2161 cccgacggaa gccgctgtat gatcgagatt gcatatgtac cgcaattcgc aaactgggga
     2221 gatattgcac aaatgatctc tgaagattgg cgcggaatcg gaatcgacgg tgttgtggtt
     2281 gaaaatgaac gaaccaacca tttcgcaaaa cgcgattcca acgaactcat gaccgaaatc
     2341 tggaatgaag acactaccgg attccccttc accggtgcgc caaaattcga tcctcggacc
     2401 agcccgggta tttgtgttgc cacgggtgct cgtacgtgga ttaattccgg tggaaaagaa
     2461 ggttatgaac ccaatgccga tttcaaacgg attatggaaa ttatcgattc tgccaagaga
     2521 gtaagccccg ataaacaggc tgaactcggt aaggaactgt tccgaatttg ggcggaaggc
     2581 tgctatgaga tcggaatcgc aggcttaacg cctcttgttc aaggtgtggc ggttattaac
     2641 gtcaacctga agaacgttcc tgcttccaac gaactcggta acgactggcc gctccgaacc
     2701 cccggaaacg cacgaaccga acaattctat tttgcaaagt aatacaaata attatggtcg
     2761 ggcgattttg caaaatcgcc cgaccggcaa ttttgaactt tattattctt tttattggaa
     2821 cgtacgagta agtgatttcc ataaagaaaa tcataagact caaaattttt atgtgatttt
     2881 tgaacggagt aaacgatggg aaaattttta gcgaagagac tccttttgat accattttta
     2941 ttgctcatat tttcgatcat aatttttgcc ttgattcagg cacctcccgg cgattttatg
     3001 accacttatt ccgcaatatt ggccggatcc ggctcatcgt tggatgccga gcagttaaat
     3061 gcgatgcggg tatcttatgg tttggatcaa ccgatgcata tacagtacat ccgatggctt
     3121 ggaaaacttc ttcacggaga tatgggattg tcattattat atatgcttcc caataagcaa
     3181 ttaatcggtg aaagattagg accgactatc cttttagcgc tgttttcatt cattctttcc
     3241 tggctgatag cgattccgtc cggaatttat tccgcgacgc ataagaattc gtttggcgat
     3301 tatttcttta tggtaatcaa ctatatcgga gtcgccattc ccaacttctt tctggcactt
     3361 ttattaatgt ggtttttcta ttctcattac ggtttaaatc tagtcggctt gttttctccg
     3421 gagtttgaaa tggcgccgtg gagttttgcg aagtttctgg atatgttgca acatatctgg
     3481 ttgcccgcta ttatcttggc attgggaggc tcagccagat taactcgggt aatgcgtgcg
     3541 aatctgttgg atgaattagg gaaaccttat atgattacgg gtcgcgccaa aggaatgtca
     3601 gaatgggcat taattatgaa gtatccggtt cgcttggcaa ttaatccgtt agtcagtaca
     3661 atcgggaact atattcccca gttgttttcc ggttctctga ttgtggcaac agtaatgaat
     3721 cttcagaata tcgggccgct ccttttgcag gcgctatcga tgcaagacat gtatttatca
     3781 gggtcaattc tgatcatata ttgtctgctt ggtattttag gaacaattct gtccgatttg
     3841 ttgttagctt gggttgatcc tcggatcaga ttggaatagt gaagatgaca gaaaattact
     3901 ctcccaaaaa taattcaaat cccacaaaaa acaatccgtc tgaatcctct gttgagaacc
     3961 aagcgaatat tgatttggat gctttggcca gcacaaaaag tgaagaaaaa gtctccatcg
     4021 cttcgaacgg acaactgatt tgatggcgtt ttcgaaaaaa taaaatggcg gtaatcagcc
     4081 tttttatttt agtaatcgtt tttgctatca ttttattccc tgattttttc gcaaatcaaa
     4141 attctgaaaa gaccaatgcg cgtgaggctt acattcccgt ccaaagttta cactttttca
     4201 aagatgggaa acccgatctt tgggtcgata aagttgaagg acaaagaaac ccgaaaactt
     4261 tgggaatgga atgggttgtc aaggaaggtg aatcggtcga cgtcaagttt tttggaaaag
     4321 gattgaaatg gaaattcctt ttttgggaaa ccgaacgtca tttaattaca ccgtcggatc
     4381 cgaatactcg cgtatttctt ttaggatcgg accgtatggg gcgggatcag ttttcgagat
     4441 tgatgtatgc gacccaaaca tctcttacag tcgggcttgt atccgttctt ctcagtgtat
     4501 ttctgggtgt cctgttgggt ggaatcagcg gatatttcgg tggaatgacc gatatggtca
     4561 ttcaaagatt gatcgaaata ttaaactcta ttccatatgt acccatttgg atggcaatga
     4621 ctgcggcgtt gcctcgtcaa tggtctgccg aacagcgttt ctttgctatt accgtaattc
     4681 tttctctgtt gggttggaca acgctcggaa gagaagttcg gggaaaattt atgtctattc
     4741 gtgaggaaga ttttatcctt tctgccgagt tgatcggatg cagtaaatgg cgaatcatta
     4801 tgaaacatat ggttcccact tttctcagcc atattattgc aaccggaacc ttatcgattc
     4861 cggcaatgat cgtaaatgaa acctcattaa gctttttagg attaggcctt cgtccgccgg
     4921 cgatcagtta tggtgtcatg ttacaggaag cacaaaatgt tcagacgatg gctaaagctc
     4981 catggctgat gctccccggc ttggtactgg taatcaatgt tttaatgttc aatttgattg
     5041 gcgacggact acgtgatgct gccgatccgt acagtaaata ggagaaaaaa tggaaaatac
     5101 aaaaccatta ttatctgtac gagatttgaa agtttatttt gatttggacg aagggacagt
     5161 caaatccgtg gatggcatct catttgatgc ttacgaaggg aaagtggtag gtattgtcgg
     5221 agaatccggg tgcggaaaat ccgttaccat gaaatcgatc ctgcgaattg tcgaaaaacc
     5281 cggaagaatc gttgaaggcg acattttgct ccaaaaaaga gataatccgg atgaatacct
     5341 taacttggtc aaaatgaaag aaaacggcaa agagattcgt gcggtccgag gaggagaaat
     5401 tgctttgatt cctcaggaac cgatgtcggc attcagcccg attcatacca tcggcgatca
     5461 aattaccgaa gcgatcatgc tgcataatcc ggttgataaa gaggaagcgc ggagaatatg
     5521 tattgaatcc ttaaaatccg tcggtatgtc tatgccggaa aagcgaatcg attcttactc
     5581 ttggcaactt tcgggcggac tcagacaaag agcaatcatc gccatggcta ttgtttgtaa
     5641 gccacggtta ttgattgctg atgaaccgac tacggctatt gatgtaacca cacaagctca
     5701 agttttgaag ctattaaaaa aactccagat ggaaagaaat atgggaattc ttttcattac
     5761 acatgatttg ggggtaattg ctcaaatggc acaatatgtt gtcgtgatgt atttgggtag
     5821 gatcatggaa aaaggaccgg ttgattcgat attccataat cccaaacatc cttatacaca
     5881 ggcattactc cgttcgatcc cttcgattca aaagacaacg aaagttaaat tgccgacaat
     5941 cagcggttca attcctcatc cgttcaataa
//
LOCUS       DAOMIV010000058         5858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_13794_length_5858_cov_4.214544, whole genome shotgun sequence.
ACCESSION   DAOMIV010000058 DAOMIV010000000
VERSION     DAOMIV010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13794_length_5858_cov_4.214544"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..947)
                     /gene="deoC"
                     /locus_tag="PKV59_02355"
     CDS             complement(114..947)
                     /gene="deoC"
                     /locus_tag="PKV59_02355"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQP45624.1"
                     /translation="MNVNSYKYDDEYDQYTDPYNCDLEMIIEEAEGYQVITTYNAEGV
                     IVDVQAHSAEESAEKSSVEKLAEMIDHTLLKADATPDQIMTLCKEARENHFASVCVNS
                     CWTRLCAEILRGSGVKVCSVVGFPLGAMIPEAKAFEAECALENGASEIDMVINIGALK
                     GRDLRLVSKDIQGVVEIAHQSGAIVKVIIETCLLTEEEKIIACLICKTANADFVKTST
                     GFSTGGAKVEDIELMRRVVGDEMGVKAAGGIHTREEAEKMIAAGATRIGASAGVKIIH
                     E"
     gene            1201..2166
                     /gene="yvcK"
                     /locus_tag="PKV59_02360"
     CDS             1201..2166
                     /gene="yvcK"
                     /locus_tag="PKV59_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HQP45625.1"
                     /translation="MDHDTKIVVIGGGHGLSTILRGLKRISENITAVVTVSDDGGSSG
                     ALRRDLGVLPPGDIRNCLAALSDDEEFLSQVLQYRFSSETSVGGHNLGNLLLTALANI
                     TGSFELGIQELTRILAIHGKVTPSTLEDVNLVADVEIENNGKKECIRVFGESNITEKN
                     GTICNLEIEPKNVIANPEAIKAILAADLIALGPGSLYTSVLPNLRVNGIAQALKASHG
                     KIIYVCNVSTQPGETTNFTCYDHVKVIHNAIPDVVIDGVVCNNYHEAAESKNISWVKI
                     EESILREYRVFQDDLIDKTYPWRHDSNKVAEALLKAYKELGSTEN"
     gene            2215..3270
                     /gene="gap"
                     /locus_tag="PKV59_02365"
     CDS             2215..3270
                     /gene="gap"
                     /locus_tag="PKV59_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQP45626.1"
                     /translation="MKVQVGINGFGRIGRQVLKSFIDNYSDELEVVAINDLFDTKTNA
                     HLFKYDSTYGKFDGSVDVVGNDLVINGKTIKVFAEKDPGSLPWKDLGVDIVVESTGIF
                     TDAIGDPEKGKPGANVHITKGGAKKVIISAPAKNEDITIVMGVNDDKYDPAKHHVVSN
                     ASCTTNCLAPVAKIINDAFGIEYGLMTTIHSYTNDQVILDQGHKKEMRRSRAAGLNII
                     PTTTGAAKAVALVIPELKGRFDGYSLRVPTPTVSIVDFVCNISKSTTVEEVNDLFRSM
                     AGKGRLKGILGVTEGTYADPLVSTDFRGDSRSSIVDLMQTSVMGGKMLKIVSWYDNEW
                     GYSNRACDLALMMSKSL"
     gene            3441..4616
                     /locus_tag="PKV59_02370"
     CDS             3441..4616
                     /locus_tag="PKV59_02370"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQP45627.1"
                     /translation="MFNKKTIRDIDVKGKKVLVRVDFNVPIKDGQVGDDTRIRGALPT
                     IQYLLDQGAAVILCSHLGRPKNGPEEKYSLKPVAEYLGTLIPNKVYFASDCIGEPAKK
                     AAENLKPGEVLVLENTRFHPEEEKNDLEMAKQLADLADIYVNDAFGTAHRAHASTEGV
                     THFKPGVAGFLLEKEIQYLSGAVENPKRPFVAILGGAKISDKIGVIKNLLNKADKVLI
                     GGGMANTFFKAQGFDMADSLVEDEAVETAKEILNMGSNKLVLPVDMVIANAFDNNAER
                     KTIPVGNVPAGWRVLDIGPETIKVFSNLIKNAATVVWNGPMGVFEMPNFAKGTFEIAK
                     AVAESNSISIIGGGDSVAAIQESGLADKITHISTGGGASLEMLEGIVLPGVAALQDK"
     gene            complement(4684..5523)
                     /locus_tag="PKV59_02375"
     CDS             complement(4684..5523)
                     /locus_tag="PKV59_02375"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HQP45628.1"
                     /translation="MNSMKIGCHLSVAQGYLHMGKEAISIDANTFQFFTRNPRGWKTK
                     ELNIQDMEDLTSLMKSNDFSPILAHAPYTLNPCAADERVHEFALMVMKEDLAIMEHMP
                     ENYYNFHPGSHVGQGIEAGIQLIIEALDHMLYMEQKTTVLLETMSGKGSEVGSTFEEL
                     QEIIKRVKLNDKLGVCLDSCHIYSAGYNIVEDLDGVLEKFDKVIGLNRLKAIHLNDSM
                     TPFGSKKDRHAKIGQGTIGLDAIIRFINHPLLKNLPFYLETPNDVPGYAEEIKILRQA
                     YNE"
     gene            complement(5520..>5858)
                     /gene="ricT"
                     /locus_tag="PKV59_02380"
     CDS             complement(5520..>5858)
                     /gene="ricT"
                     /locus_tag="PKV59_02380"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQP45629.1"
                     /translation="LTDFSSISIKMAKEQGISLTPSEITGMCGRLRCCLEYENEYYAE
                     CRKNLPKKKKKVITPQGEGKVVEIFPLRHSVLVEIPCVGRREFTADQIKYDEIPAKQD
                     DSSKEEEEAK"
BASE COUNT         1708 a         1203 c         1184 g         1763 t
ORIGIN      
        1 tatccgtcat cccgtgtaaa aatacaaccg gccgaagccc taaacttgtc ctggcataat
       61 gcaatttaat gccgccagat tcgatatttc cataataaag tttttcggac gattcattca
      121 tggataattt ttactcccgc acttgctcca atacgcgtcg caccggctgc aatcattttt
      181 tccgcttctt ctctggtatg aattccgccg gcagctttta cacccatttc atcaccgact
      241 acacggcgca tcaattctat atcttcaact tttgcgcctc ccgtcgaaaa accggttgaa
      301 gtttttacaa aatcagcgtt cgcggtttta catatcaagc aagcaatgat tttttcttct
      361 tcggttaaaa gacaagtttc gataatcact tttacaatgg ctccggattg atgagcgatt
      421 tcaacgacac cttgaatatc ctttgaaacc aaacgcaaat cccgtccttt caaagcaccg
      481 atatttatca ccatgtcgat ttccgatgcg ccgttttcta aagcgcattc cgcttcgaat
      541 gcttttgctt ccggaatcat agcgccgaga ggaaatccga caacagaaca aactttcaca
      601 ccgctgcccc gaagaatttc agcgcacaat ctcgtccaac aagaattgac acaaacggag
      661 gcaaaatgat tttcacgtgc ctctttgcac aaagtcataa tttgatccgg tgtcgcatcc
      721 gcttttaaca acgtatgatc aatcatttcc gccagttttt caaccgatga tttttccgca
      781 ctttcttctg cggaatgagc ttgaacatct acaatcacac cctctgcatt ataagttgtt
      841 ataacttgat atccttcagc ctcttcgatt atcatttcca aatcacaatt atatggatct
      901 gtatattgat catattcatc atcatattta taactattca cgttcataga aacctcgact
      961 ttttttgtta ttatatcagg tcagttccgg caatttgcgt tccggatggg aataatcgcg
     1021 aaaccggatt ctaaatttct tctcatcaaa tctttatttt cttttcaatg atctttcccg
     1081 agttcaggaa tatatttata aataaatgag gatgtgtttg tcaggtcttt acattcgatc
     1141 tcttgaatgc tattatttgg accaatggaa gatcataatc agttcgataa atatccaaaa
     1201 atggaccatg acacgaagat agttgtaatc ggaggtggac atggtttgtc aaccattctc
     1261 cgtgggttaa aaaggatttc agaaaatatt accgcggttg ttaccgtctc ggatgacgga
     1321 ggttcttccg gcgctctcag gcgagatctg ggtgttcttc ctcccggtga tattcgaaac
     1381 tgcctcgccg ctctatcgga tgatgaagag tttttatccc aagttcttca atatcgcttt
     1441 tcttcggaaa caagtgttgg aggtcataat ttaggaaatt tacttttgac cgctttggca
     1501 aacattacag gaagttttga actgggaatt caagaattaa ccaggattct tgcgattcac
     1561 gggaaggtta ctccctctac attggaagat gtcaacctcg ttgcggatgt tgaaattgaa
     1621 aacaacggga aaaaagagtg cattcgagtt ttcggagaat ccaatatcac agaaaaaaac
     1681 ggcacgattt gcaaccttga aattgaaccg aaaaatgtaa tcgccaatcc ggaagccatt
     1741 aaagcaatat tagctgcgga tctaatcgca ttgggtcccg gcagtcttta tacaagtgtt
     1801 cttccgaatt taagagttaa cggtattgct caggcattaa aagcaagcca cggaaagatt
     1861 atttatgtat gcaatgtttc cactcaaccc ggcgaaacga ctaatttcac ctgttatgat
     1921 catgttaaag tcatccataa tgcaattcca gatgtcgtga ttgacggtgt agtttgtaat
     1981 aattatcacg aggcagcaga atccaaaaat atttcctggg ttaaaattga ggagtcaatt
     2041 ttaagagaat atcgtgtttt tcaagatgat cttatcgata aaacatatcc ttggaggcac
     2101 gactcaaata aagttgcgga agctctttta aaagcttata aagaattagg ttccacggaa
     2161 aattagtggc tcattaaata aattttaatc ttttatgcaa ttttggagga atcaatgaaa
     2221 gttcaggtag gtattaatgg ttttggacga atcggccgac aggttctcaa atcattcatc
     2281 gataattatt cggatgaatt ggaagtagta gctattaatg atctttttga tacaaaaacc
     2341 aatgcacatc ttttcaaata tgattcaacc tatggaaaat ttgacggatc cgttgatgtc
     2401 gtaggcaacg atctggtcat taatggcaag acgattaaag tttttgccga aaaagatccc
     2461 ggatctcttc cttggaaaga cttgggtgtc gatatcgtcg tcgaatctac cggtatattc
     2521 acagatgcaa tcggtgatcc cgaaaaaggg aaacccggtg ccaatgttca tataacaaaa
     2581 ggcggagcaa agaaagttat catttcagcc ccggcaaaaa atgaagatat caccattgta
     2641 atgggagtta atgatgataa atatgatccg gcgaaacatc atgtggtttc taatgcttcc
     2701 tgtacaacaa attgtttagc accggttgca aaaattatca atgatgcgtt tggtatcgaa
     2761 tatggtttga tgaccacgat tcacagctat acgaatgatc aggttatctt ggatcaaggt
     2821 cataagaagg aaatgcgtcg ttcacgcgct gccggtttga atattattcc gactacaacc
     2881 ggagctgcga aagcggtggc attggtcatt cccgagctaa aaggacgttt tgacggttat
     2941 tcgttacgag ttccgactcc gactgtatca atcgtcgatt tcgtttgcaa tatctctaaa
     3001 tcaacaacgg ttgaagaagt aaatgattta ttccgcagca tggctggcaa aggaagatta
     3061 aaaggcattt taggagttac cgaagggaca tatgccgatc cgttggtttc gactgatttc
     3121 cgcggtgatt ctcgatcttc aattgtagat ttgatgcaga catccgtaat gggcggaaag
     3181 atgctcaaga ttgtatcttg gtatgacaat gaatggggct attcgaaccg agcttgtgat
     3241 ctggctttga tgatgtcgaa gagtctctga ttcatctgaa tgttttgaag gaataaaaat
     3301 gttatccgca tcaaaaagtg cgggaacttg aaagagaaaa tgatcggaac tgcaaaaact
     3361 ttcagccgat cattttttct tataagtcgc tgatttgctt gataaaaact tatcgttatt
     3421 cagaagaaag ggaaacgaac atgtttaata agaagaccat acgcgatatt gatgtaaaag
     3481 ggaaaaaagt tcttgtcaga gttgacttca atgttcccat caaagatgga caagtcggtg
     3541 atgatactcg aattcgcgga gctttaccga caattcagta tcttttagat cagggtgccg
     3601 ctgtcatcct ttgctcacat ctcggacgtc ctaaaaacgg tccggaagaa aaatattctt
     3661 tgaaacctgt tgccgaatat ctcggaacat taattcccaa taaggtctat tttgcttcgg
     3721 attgcatcgg tgaaccggca aagaaagcgg ctgaaaattt aaagccgggt gaagtactgg
     3781 ttctcgaaaa tacccgtttt catcccgaag aggaaaaaaa tgacttggaa atggcaaagc
     3841 aattggcaga tttagctgat atctatgtta atgacgcttt cggaactgca catcgagctc
     3901 atgcctcaac cgaaggagtt actcatttca agcccggagt agccggcttt cttttggaaa
     3961 aggaaataca atacctttcc ggagctgtgg aaaatccgaa acgtccgttt gtcgcaattt
     4021 taggcggcgc taaaatctcg gataaaatcg gcgtgattaa aaatctgctg aacaaagcgg
     4081 ataaagttct catcggcggc ggaatggcaa ataccttttt taaagcacaa ggatttgata
     4141 tggctgattc tctggttgag gatgaggcag tggaaaccgc taaagagatt ttaaacatgg
     4201 gcagtaacaa attagtgttg ccggttgata tggttatagc caatgctttt gataacaacg
     4261 ctgaaagaaa aactattcct gttgggaatg ttcctgccgg ctggcgtgtg ttagatatcg
     4321 gtcctgaaac cataaaggtt ttcagcaacc tgattaaaaa tgccgccaca gtggtatgga
     4381 acggtccgat gggtgtcttc gaaatgccta attttgcgaa agggacattt gaaatcgcaa
     4441 aagcagttgc ggaaagtaat tctatttcca ttatcggcgg aggagattct gtggctgcga
     4501 ttcaggaatc cggtctcgcg gataaaatca ctcatatttc aacgggcggt ggagcttctc
     4561 ttgaaatgct cgagggtatt gttcttcccg gtgtcgcggc actacaagat aaataatctt
     4621 ctcaactgtt aaaaagaaaa atgccggacg aaaaaaaaga tccggcattt tttattaatt
     4681 catttattca ttataggctt gccgcaatat ctttatttct tcggcatatc ctggaacatc
     4741 attaggggtc tctaagtaaa acggtaaatt ttttaatagc ggatgattga taaaacgaat
     4801 aatagcatcc aacccgatcg tcccctgacc aatttttgca tgacgatctt ttttactccc
     4861 gaacggtgtc atactgtcat tcaaatggat cgccttcaac cggtttagtc cgatcacctt
     4921 atcaaatttc tccaacacac cgtctaaatc ttccacaata ttgtatcccg ccgaataaat
     4981 atgacagctg tccaaacata ctccaagttt gtcattcagt tttactcttt taataatctc
     5041 ttgaagttct tcaaaagtac tgcctacttc actgcctttt ccggacatgg tttccaataa
     5101 gacagttgtt ttttgctcca tatataacat atgatcgagc gcttcaataa tcaactggat
     5161 accggcttcg attccttgtc cgacatggct cccgggatga aaattataat aattctccgg
     5221 catatgttcc ataatagcca gatcttcctt catcaccatc aaagcgaatt catgtacacg
     5281 ttcatccgct gcgcaggggt ttagggtata aggggcatgg gctaaaatcg gagaaaaatc
     5341 gtttgatttc atcagacttg tcaaatcttc catatcttga atattcaatt ctttggtctt
     5401 ccagcctcgc gggttccgcg taaagaattg aaaagtattc gcatcgatac tgatagcttc
     5461 tttacccata tgaagataac cttgcgcaac cgacagatga caaccgattt tcattgaatt
     5521 cattttgcct cttcttcctc ttttgaacta tcgtcttgtt tagcgggaat ttcgtcatat
     5581 ttaatttggt ctgcggtgaa ctccctcctc ccgacacatg ggatttcaac aagtacggaa
     5641 tgtcttaacg gaaagatttc gacaaccttc ccttcaccct gaggagtgat gacttttttc
     5701 ttcttcttgg ggaggttttt ccgacattca gcataatatt cattttcata ttctaaacaa
     5761 catcgcaatc gtccgcacat tccggtaatt tcggaagggg taagcgatat tccttgctct
     5821 ttggccatct tgatcgaaat actgctgaaa tcagtcag
//
LOCUS       DAOMIV010000059         5853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_13803_length_5853_cov_4.147982, whole genome shotgun sequence.
ACCESSION   DAOMIV010000059 DAOMIV010000000
VERSION     DAOMIV010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13803_length_5853_cov_4.147982"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1179
                     /locus_tag="PKV59_02385"
     CDS             172..1179
                     /locus_tag="PKV59_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP45630.1"
                     /translation="MRFLIAGFGSIGRRHLRNLRACGQNDLVLLRSNHSTLPTDEIGD
                     IPVETEIIKAMAHHPDGVIIANPTAFHLDVAIPAAKAGCSILMEKPITDQIEKIGELE
                     SALKAGNGKLLMGFQFRFHPGLIKIKELMQTGAIGRPLSFQACWSEYLPDWHPWEDYR
                     KSYSARKDLGGGVALTLCHPFDYVRWLFGEVDFIWGYNGKISDLELSVDDVAEVGVKM
                     ENNLVGSIHLDYFRRFVRNDLEITGTQGVLHWNNSDACVTLQNAVYPESVIIPPAEPF
                     ERNQLFLEEMKHFIAVTKREVEPSCTLQDGLITEQLINAVKRSWDEKRIIDFKKEQNL
                     G"
     gene            1172..2767
                     /locus_tag="PKV59_02390"
     CDS             1172..2767
                     /locus_tag="PKV59_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45631.1"
                     /translation="MVSFFRKRTLFFIILWFVLLSVIFTWPLVTHLTHEIAGDIGDNI
                     YFIWMIGWVKKALFQIHANPLDVWFLNYPEGWNLARTEMAPAQLALALPFSLIGGETF
                     GYNMAMLLSFIFAGTMMFGWVRHLSGSASAGWVGGTVFACLPYWHSHFLAGHLNLCGI
                     QWIPLFFWGWFDMLRPGTGEFRKKSAILAALGLGLTAFCSQYYVFMLIFVAAIQGILL
                     ILPNRISQLKDIAFWKTFGLFLLIAVPLLILAELPFLTLASSGGMPDRDWGSASMYSA
                     SPTDFLLPATTHFIFGKWVGSHFRRDLWIEATLYAGIISLILYFYGMVRKKKEQNWIW
                     SALTIGIVCAIILAMGTDLHWNEQPVTVSLPRFLQNILQKESARIYLPGLILYKYFPF
                     FGKMRALMRFGLFALVLIACGAGLGAKALIERTGSRSIFISVLLITLIILDFAPRPID
                     RFSEIKPRPVDLWLSERPESGSVMNIPFELNDDQAATYYTLYNNKPFIGGFLMPFPHR
                     NTGALKRSWILSRARKASKKLGN"
     gene            2719..2949
                     /locus_tag="PKV59_02395"
     CDS             2719..2949
                     /locus_tag="PKV59_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45632.1"
                     /translation="MDTFPSTESLEKARELKVDFFLFDLEALDSLIGQKPTARWQTLE
                     ELKNQCRENGLVELAAFDNLSVYGFKEDDENK"
     gene            2933..4549
                     /locus_tag="PKV59_02400"
     CDS             2933..4549
                     /locus_tag="PKV59_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP45633.1"
                     /translation="MMKINNQKAIWGSAVLPAVLGCYLLAISMRRGPGVGGDATIYIT
                     AARNLLAGKGLGLINPAGEFRLLPYFPPFYPLLLAFFGWLGFDPAGFAGPFNMILFAV
                     TVFVVAKTISQSAEKWIAGLLTGTLLAASPILIPVYSWAMSEPLSIALMAIGFLTLAQ
                     YLSTGQRNRLFISAVSCGLSFLTRYSMAACLICACLILLFAQKKQFRIRLSEAVVYGL
                     IAFLPMLIWLIYDIRMTSTVASRSILENVNLWEEIRRFNSQLQPVFLQWLFPDSIIHS
                     PSIPAWGNSMVYYLLLVLPFFICTGIPHRVTSDQNDRILNNLSMIFCSFFYIYLFVIL
                     LTSVTTYPPITIGSRMLSPAHFSFLCLIGSIYAKLLRHRQNSSGLKTIITFCLIGLAA
                     TYGLRSIRIARQNAIDGLGYNSVKWRESETVAYIRDHIPPEQPIVTNEETALLFLLNR
                     ITWPMHEVYVNRPDENFYAYESPNPQDIDAGRSAFQRGEAFLVVFDTFEDQMRDIYGQ
                     DTQKRIDALFQNLKVVADTDDGVIYTLNRE"
     gene            4553..>5853
                     /locus_tag="PKV59_02405"
     CDS             4553..>5853
                     /locus_tag="PKV59_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45634.1"
                     /translation="MLIFSGVIGLLQCVLLPGLILSKVFKIRGGLIERFLYLFPLSLV
                     ANYLVIFLLAALRIYTRPVLSLLIAFEIFALLFLFRQAFQTPLKEILLKAEKTIKQEL
                     QPLTTTFAQPYHNSEEIIQNALWVLSGLGSVSAVLWGIHVWRLNFGTIFSGWDTLFSW
                     NAFAETWATNQIPAFRGSYPQLVAANWSISYVLQGDNPVQFFNTLIPPLFFLWIFLML
                     FDLGFQKKETGFFFAAIIARYMMKKLMGDQIFNGYMDVPAASMVLLSLYAILKGSDRS
                     VKDQQQSLVLGLVFASEAAVTKQSGLIALIVVPIFIHRWLKAAEQQIPQKQKIGWIVV
                     CAGIVLPWYFGGLIQWMMNPDTDTRIVDQGIVQFNQRYEWSHKFYLIRQSLGKYFYVF
                     GISLIGLPFVPKKYRLPFFLISWPIVILWAMFFTYDTRNLAV"
BASE COUNT         1428 a         1274 c         1360 g         1791 t
ORIGIN      
        1 ctcgctggcg cagtagcagg cgaggtctgg catcggggcg catggatctg attctggtac
       61 aaaacggaga tcgttcgtac cggtataccg gcgaatcgaa atcaaaatag gaatggtttc
      121 tgctaaaata aatgcaacaa atctaacgca gcggtaaaga agggatgaaa tatgcggttt
      181 ttaattgcgg gattcggttc gatcggacga aggcatttga gaaaccttcg tgcatgcgga
      241 caaaatgatc tggtgctgct gcggtcaaat cacagcacct taccgaccga tgaaatcggg
      301 gatattccgg ttgaaacaga aataatcaaa gcgatggctc atcatccgga cggcgtaatt
      361 atcgccaatc cgaccgcttt tcatctggat gtcgccattc ccgcagcaaa agccggctgt
      421 tccatcctga tggaaaaacc gattaccgat caaatcgaaa aaataggcga attggagtcc
      481 gctcttaaag ccggtaacgg gaaattattg atgggttttc aatttcgctt tcatcccggc
      541 ttaatcaaga taaaggaatt gatgcagacc ggtgcaatcg ggcgaccgct ttcttttcaa
      601 gcctgttgga gcgaatactt gccggattgg catccgtggg aagattatcg aaaaagttac
      661 agtgcacgaa aagatttagg cggtggtgtg gctttgaccc tctgccatcc tttcgactat
      721 gttcgttggc ttttcggtga agtggatttc atctggggct ataacggcaa aatcagtgat
      781 ttggaacttt ccgttgatga tgtcgccgaa gtcggcgtga aaatggaaaa taatttggtc
      841 ggatcgattc atttggatta ctttcgacga tttgtgcgca atgacctgga aattaccggt
      901 acgcaagggg ttttacattg gaacaattcc gacgcatgcg tgacgttaca aaacgcagtc
      961 tatccggaat cggttattat ccctcccgcc gaaccgtttg aacgcaatca attgtttctc
     1021 gaagagatga aacattttat tgcggttaca aaacgagagg tcgaaccctc ttgtacgctt
     1081 caagacgggt taattacgga acaactgatc aacgcggtga agcgttcctg ggatgaaaaa
     1141 cggatcatcg attttaagaa ggaacaaaat cttggttagt tttttcagaa aacgaactct
     1201 tttttttatt attttgtggt tcgtgctcct gtctgtaatt tttacctggc cgctggtaac
     1261 tcacctgacc catgaaatag ccggggacat cggcgataat atttatttta tttggatgat
     1321 cggttgggtc aaaaaagcgc tttttcagat ccatgccaat cctttggatg tctggttctt
     1381 aaattatccg gagggttgga atctggcacg aaccgagatg gctccggcgc aattggcttt
     1441 ggcattaccc ttcagtttaa tcggcggcga aactttcggc tataacatgg cgatgctgct
     1501 tagtttcatc tttgccggaa caatgatgtt cggttgggtc aggcatttga gcggatctgc
     1561 atcggcaggc tgggtcggag gtaccgtctt tgcctgttta ccttattggc attcccattt
     1621 tttggccggt catttgaacc tctgcggaat ccaatggatc ccgcttttct tctggggctg
     1681 gtttgatatg ctgcgaccgg gtaccgggga atttcgcaag aaatccgcaa tattggcagc
     1741 tctggggttg ggattgaccg cattctgctc tcaatactat gtatttatgt tgattttcgt
     1801 cgccgccata caagggattt tgctcattct tccgaaccga atttctcaat taaaagatat
     1861 agctttttgg aaaacctttg gacttttcct attaatcgct gtgccgcttt tgatccttgc
     1921 cgagttgccg tttttgacct tggcaagctc cggagggatg cctgatcgag attggggcag
     1981 cgccagtatg tattcggcaa gtccgaccga tttcttattg cccgccacaa cccatttcat
     2041 tttcggaaaa tgggtcggca gccatttccg ccgtgattta tggattgaag ccacattgta
     2101 cgcgggaatc atttcattga ttctttattt ttacggaatg gttcgaaaga aaaaagaaca
     2161 aaattggatt tggtcagccc tgacaattgg gattgtttgc gcgataattt tagctatggg
     2221 aaccgattta cattggaatg aacagcctgt caccgtttct ttgccccgat ttttgcaaaa
     2281 tatactgcaa aaagaatctg ccagaattta tctgccggga ttgattctat ataaatattt
     2341 tccgttcttc ggaaaaatgc gcgcattaat gcgcttcgga ctgtttgcat tggttcttat
     2401 tgcttgcgga gcgggtttag gcgcgaaagc tcttatcgaa agaaccggaa gccgatccat
     2461 cttcatctcg gttctattaa tcacgctgat catcctggat ttcgccccca gaccgattga
     2521 ccggttttcc gaaattaaac cgcgtccggt cgatctctgg ttatcggaac gccccgaaag
     2581 cggttcggtc atgaacattc cctttgaatt gaatgatgac caggctgcca catactatac
     2641 tttatataat aacaaaccgt ttatcggcgg attcttaatg cctttccccc atcgcaatac
     2701 cggagcatta aagagatcat ggatactttc ccgagcacgg aaagcctcga aaaagctcgg
     2761 gaattgaaag tggatttctt cctgttcgat ttagaagcgc tggattctct gatcgggcaa
     2821 aagccgaccg ctcgctggca gacacttgaa gaactgaaaa atcaatgccg ggaaaacggt
     2881 ttggttgaat tggctgcctt tgataatctg tcggtgtatg gttttaaaga agatgatgaa
     2941 aataaataat caaaaagcaa tatggggatc ggctgttttg cccgccgttc tcggatgcta
     3001 tttactggcg ataagcatgc gccggggacc gggagtggga ggcgatgcca cgatttacat
     3061 cactgctgcc cgaaatctgt tagccggaaa aggattgggg ctgatcaatc cggcgggaga
     3121 attcaggctt cttccatatt ttcctccttt ctatccgctt ttgttggcat ttttcggttg
     3181 gcttggtttc gatccggccg gctttgccgg gccattcaat atgattctgt ttgccgtcac
     3241 cgttttcgtg gttgccaaaa caatttcaca atccgccgaa aaatggattg ccggacttct
     3301 gacgggcact ttgttggcag cttcaccgat tttgataccg gtttattcct gggctatgtc
     3361 cgaacctttg agcatcgcat tgatggcaat cggattcctt acgttggcac aatatctatc
     3421 aacgggacag cgtaaccggc tgttcatcag cgcagtcagc tgcggccttt cttttctgac
     3481 ccgttacagt atggcagctt gtttgatctg cgcttgtttg attttgttgt ttgctcagaa
     3541 aaaacaattt cgcattcgtt tgagcgaagc cgttgtttat ggtttgatcg cttttcttcc
     3601 catgctgatt tggctgattt atgatatccg aatgacttcg acggtggcat cccgcagtat
     3661 tttggaaaat gtaaatttgt gggaggaaat caggcgtttt aatagtcagc tgcagccggt
     3721 ctttttacaa tggctttttc cggattcaat catccattca ccatccatcc ccgcttgggg
     3781 taactctatg gtctattatc tgctgcttgt tcttccgttc ttcatctgca ccggcattcc
     3841 gcatcgggtt accagtgatc aaaacgaccg gattctcaat aacttgtcga tgatattttg
     3901 ttcatttttt tatatttatc tttttgttat tctgctgacc tccgtcacca cttatccgcc
     3961 gattacgatc ggatcgcgaa tgctttcacc ggcacatttc agctttctct gcttgatcgg
     4021 ttcgatttat gcaaaattat tgcggcaccg gcaaaactct tccggattga aaaccattat
     4081 cactttctgt ctgatcggtt tggctgcgac ttatggattg cgatcgattc gtatcgcccg
     4141 ccagaatgcg atcgacggat taggttacaa ttcggtcaag tggagagaat ccgaaacagt
     4201 ggcttatatt cgtgaccaca ttccgcccga acaaccgatt gtcaccaatg aagaaacagc
     4261 gctactgttt ttgttaaacc gaataacctg gccgatgcat gaagtttatg tcaatcgacc
     4321 ggatgagaat ttttatgcat atgaaagccc gaatcctcaa gacatcgacg caggcagatc
     4381 cgcttttcaa cggggcgaag cattcctggt cgtattcgat acttttgaag atcagatgag
     4441 agatatttac ggtcaggata ctcaaaaaag gatcgatgcc ttgtttcaaa atttgaaagt
     4501 agttgcggat acggatgatg gcgttattta taccttaaac agggaatagt tcatgttaat
     4561 tttttccgga gtcatcggac tgcttcaatg cgttttgtta ccgggattaa ttctctcgaa
     4621 ggtttttaaa attcgcggag gattaattga acgttttctc tatttgttcc ctttaagtct
     4681 ggttgccaac tatttggtca tttttctgct tgctgcttta agaatttata cccgaccggt
     4741 tctatcgctt ttgatcgctt ttgaaatctt tgccttgttg tttctttttc ggcaagcatt
     4801 ccaaaccccg ctgaaagaga ttcttttgaa ggctgaaaaa accataaaac aggagctgca
     4861 gcctttaaca acaacttttg cccaacctta ccataacagc gaagaaatta tacaaaatgc
     4921 actgtgggta ttaagcggtc tcggttccgt ctccgccgtt ttatggggga tccatgtctg
     4981 gcgcttgaat ttcggaacga ttttcagcgg atgggatact cttttctcgt ggaatgcatt
     5041 tgcagaaaca tgggcaacca accaaattcc ggcttttcgc ggatcctatc cgcaacttgt
     5101 cgctgccaat tggtcaataa gttatgtgct gcagggtgac aacccggttc aattctttaa
     5161 cacactcatt ccgcctttat tctttctgtg gattttcctg atgctttttg atctgggttt
     5221 ccaaaagaaa gaaaccggct tttttttcgc tgcgattatc gcacgatata tgatgaaaaa
     5281 attgatgggg gatcagatat ttaatggcta tatggatgta cccgcagcca gcatggttct
     5341 tctgtccctt tatgcaatct taaaaggcag cgatcgatcc gtaaaagatc agcaacaaag
     5401 cctcgttctc ggattagtct ttgcttccga agcagccgta accaaacaat ccggactcat
     5461 tgcgctgatt gtagtaccca ttttcattca tcgatggctg aaagctgccg aacagcaaat
     5521 tccgcaaaaa cagaagatcg gatggatagt tgtttgtgcc gggattgttc tcccttggta
     5581 tttcggcgga ctcattcaat ggatgatgaa tcctgatacc gatacccgga ttgttgatca
     5641 agggattgtt caatttaatc aacggtatga atggtcgcat aaattttatt taatccgaca
     5701 atcgcttggc aagtacttct atgtttttgg gatcagtttg atcgggcttc catttgttcc
     5761 gaaaaaatat cggcttccgt ttttcttgat atcctggccg atcgtcatcc tttgggctat
     5821 gttttttact tatgatacgc gaaatctagc ggt
//
LOCUS       DAOMIV010000060         5811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_13948_length_5811_cov_4.277797, whole genome shotgun sequence.
ACCESSION   DAOMIV010000060 DAOMIV010000000
VERSION     DAOMIV010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13948_length_5811_cov_4.277797"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PKV59_02410"
     CDS             complement(<1..476)
                     /locus_tag="PKV59_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQP45635.1"
                     /translation="MEYRTFAELIHTVKNKPRKARLAVAAAGDEHTIEAVLHARDEEI
                     ILPVLVGDKAEILTVLEKLGKNIPAEDIYDVPDPAESARVAVDLVRRGGADFLMKGRM
                     ETAVLLKAVVDKEHGLSRGGIMSHFTVFEVPTYHKLLAPVDGGMVTYPDLQQKKCI"
     gene            complement(546..1127)
                     /locus_tag="PKV59_02415"
     CDS             complement(546..1127)
                     /locus_tag="PKV59_02415"
                     /inference="COORDINATES: protein motif:HMM:NF005325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HQP45636.1"
                     /translation="MSEVKNILLCGVGGQGTILASKILSTALVAAGYDVKMSEIHGMS
                     QRGGSVVTQVRYGDKVYSPIIGKGSADILVSFEAMEALRYLDGLKKDGVAVVNDYRIP
                     TATTLSGQETYSAGCIEKLKTLVNTKVLDAAKIATELGNPKSMNIVLLGALIKLMKLE
                     ALDWDAALTDNIKPRFVTVNRKALEAGMAAVAE"
     gene            complement(1120..2892)
                     /gene="iorA"
                     /locus_tag="PKV59_02420"
     CDS             complement(1120..2892)
                     /gene="iorA"
                     /locus_tag="PKV59_02420"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HQP45637.1"
                     /translation="MKQLMTGNEAVARGAWEAGVRYASAYPGTPSTEILENIAQYREI
                     YSEWAPNEKVATESAIGASFAGGRALAAMKHVGLNVAADPLFTGAYIGVNGGLVIVSA
                     DDPGLHSSQDEQDNRYYARAAKVGMLEPSDSQEAKDFVGIALELSEQFDMPILLRMTT
                     RVCHSKSIVDLSKERTEKPSVPYAKQDKFNPIPAISKRLHTKVEQNLKRLEEYSESSP
                     LNYVEWGEKKIGIISSGVAYEYAKEVYGENASYLKLGFTFPLPMRKIREFAARVDQLI
                     VVEELEPIMENEIKAAGIPCIGKEKIPCEGELNPDILRQSLLGEEHETIRYDASLVAA
                     RPPVLCAGCPHRGFFYELQKMKNVITVSDIGCYGLAPKDIAICMGGGFSVAHGAQKIF
                     NSESDGTRKRCVGIMGDSTFFHSGMTSMLEAVYNRSDILMVVLDNRITAMTGHQQNPG
                     TGLTLAEEPTHMTDIAEVAQALGVKHIRTVNPLKPDDVRDALKWGMGFDEPALLITRW
                     PCALKKMTQTDVEEFGSLRFVNRVDPEKCIGCRACIQTGCPAIEYHADSKKVTIDQIQ
                     CTGCDLCAQVCPKHAIFREEKQNV"
     gene            3102..3752
                     /locus_tag="PKV59_02425"
     CDS             3102..3752
                     /locus_tag="PKV59_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQP45638.1"
                     /translation="MIKKNSLVDQVYEELRNGIISLRIPLGSRLNVNELQDTLQVSCT
                     PIREAINRLQQEGLVTYENNVGARVLNLDEHDVEEIQQLAMTLHREAIRLAMEREDHA
                     ILLSELEQHLSEYVDAETVQDEVMAVNHLVGTFYHHCGNRRLDKSMISIQGQQLLLRY
                     IFATYRKDRKSGSDFAPLIQAVRESDVEAACRALQQNADGNTPVILDYLRKRNANG"
     gene            complement(3811..5187)
                     /locus_tag="PKV59_02430"
     CDS             complement(3811..5187)
                     /locus_tag="PKV59_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45639.1"
                     /translation="MAIPFYAKSTGNEGFIKNMEVVGFSNLNGVNAFQMALYKTADGK
                     YYMYCGSFRGPGWNIVDVTDPAHPTVVNWLEACDPKEFPATNTPKIQVADGLMIGALG
                     GGVPFLHGNKPGDKNKSGLMIFDIRQDPIHPKLLSFWDTGVKDGMGVHRFMYNGGRYV
                     HLSADCPGFTGNIYRILDIIDPTHPTEVGRWWDPRQFTDGLREGEYPVDGAQHWEFMD
                     WPQLHGPPFAVGNLAYLSYYSEGLIILDISDITRPKKIGQLKLKGPFSGKYAGARTHT
                     CLPLPGRNFLVATNEGERFPFYNKKILSEGPGKGAQPMNNLHMIDISDPSDPQLIAEF
                     PYPEVPENFPYRNFNTAGLDGAQGPFGPHNIHEPMSGKPWLDQNADRIYCCYFHAGMR
                     VYDVSDPYYIKELAYFIPPNPEKLLYEIPVPGPMLATTEDCVVDDRGFIYLDTWQDGM
                     YIVRVKNT"
     gene            complement(5221..5697)
                     /locus_tag="PKV59_02435"
     CDS             complement(5221..5697)
                     /locus_tag="PKV59_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45640.1"
                     /translation="MFRKDQFGLVFNTIFCLMFAIFIPLFMEWQNGVLGLEAFCKDFI
                     PGFTVALTVATYIDLKAMGDSFARKCGVKNENGLLFHILRVASITFVMTAIMTVVMTF
                     LAVGFSLGPVFFLVCLKAFPAVYAVALVVAFITFAFGMKLTAALCVKPPKSMPAFN"
BASE COUNT         1472 a         1453 c         1357 g         1529 t
ORIGIN      
        1 ataatacatt ttttctgttg cagatcggga taggtaacca ttccgccgtc caccggtgcc
       61 agcagtttgt gataggtggg aacttcaaag acggtaaaat gggacatgat tccgccacga
      121 ctcaacccgt gctccttgtc gaccaccgct ttcaataaaa cggccgtttc catccgacct
      181 ttcatcagga aatccgctcc gccccggcga accaagtcca ccgccacacg ggcggattcc
      241 gccggatcgg gaacatcata aatatcttcc gccgggatgt ttttccccag tttctccaag
      301 acggtgagga tctccgcctt gtcgccgacc aaaacgggaa gaataatttc ttcgtctcga
      361 gcgtgcagca ctgcctcaat cgtatgttcg tcgccggcgg cggcgaccgc cagcctggct
      421 tttcgaggct tgttctttac ggtatgaatt aactcagcaa atgttctgta ttccatggag
      481 ttccttttac ccgaaggacc ggctcgattg aaccggtacc ggtttcgacc gaggatcttt
      541 attcatcact ccgcaaccgc agccattccg gcttccaaag ctttgcggtt gacggttaca
      601 aagcggggct taatgttgtc ggttaatgcc gcatcccaat ccaacgcttc cagtttcatc
      661 agttttatca atgctcctaa caaaacaata ttcatgcttt tgggattgcc taattcggtt
      721 gcgatctttg cggcgtcgag cactttggtg tttaccagag tcttcagttt ttcgatgcaa
      781 cctgccgaat aggtttcctg ccccgaaaga gtggtggctg tcggtatccg ataatcattg
      841 accactgcca ctccgtcttt ttttaatccg tctaaataac gcagggcttc catcgcctca
      901 aaggagacca aaatatccgc acttcctttt ccgatgatgg gagaataaac cttatctccg
      961 taacgtacct gagtgacgac actgccgccg cgttgactca ttccatgaat ttcactcatt
     1021 ttaacgtcat aaccggccgc taccaaggct gtcgaaagga tcttgctggc taaaatggta
     1081 ccttgaccgc cgacaccgca aagtaaaata ttttttactt cagacatttt gcttttcctc
     1141 cctgaaaatg gcgtgtttcg gacaaacctg cgcacataaa tcacagcctg tgcattgaat
     1201 ttgatcgatg gtgactttct ttgaatccgc atggtattcg attgccgggc agcctgtttg
     1261 aatacatgcc cgacatccga tgcatttttc cggatccaca cggttgacga agcggaggct
     1321 gccgaattcc tcaacgtccg tttgagtcat cttcttcagc gcacagggcc accttgtaat
     1381 aagcagagcg ggttcatcaa atcccattcc ccatttcaga gcatcccgaa cgtcatccgg
     1441 cttcaacggg ttgacggtcc gaatatgttt gacccccaac gcttgcgcga cctccgcgat
     1501 atcggtcata tgggtgggtt cttctgctaa ggtcaagccg gttcccggat tttgttggtg
     1561 accggtcatg gccgtgatgc gattgtctaa aacaaccatc agaatgtcgg agcgattgta
     1621 aaccgcctcc agcatggaag tcatcccgga atgaaaaaat gtggaatctc ccattatccc
     1681 cacacaacgt tttcgcgtac cgtctgattc tgaattgaat attttttgag cgccgtgcgc
     1741 gacggagaaa ccgccgccca tacaaatggc gatatccttg ggtgccaaac cgtaacaacc
     1801 aatatcgctg accgtaatca catttttcat tttttgcagt tcatagaaaa agccgcgatg
     1861 cgggcaaccg gcacagagaa cgggcgggcg ggcggcaacc agcgaagcat catagcggat
     1921 tgtttcgtgt tcttcaccca acaatgattg ccgcaggata tccggattta attcaccctc
     1981 acacggaatt ttttctttcc cgatgcaggg gatgccggcc gctttgatct cgttttccat
     2041 gatgggttcc aactcttcga ctacaatcaa ttgatcgacc cgggcggcaa actcacggat
     2101 ttttcgcatc ggcagcggaa atgtaaaacc gagcttcaga taggaggcat tttcaccgta
     2161 aacctctttg gcatattcat aagcgacgcc ggaggagata atgcctattt ttttctcgcc
     2221 ccattcgaca tagtttaatg gggaactctc ggaatattct tccaaccgtt tcagattctg
     2281 ttccactttg gtatgaagcc gtttggaaat tgccggaatg ggattgaatt tatcctgctt
     2341 ggcatagggt accgagggct tttccgtccg ttctttactc aaatctacaa tacttttgga
     2401 atggcacact cgagttgtca ttcgtaaaag aatcggcatg tcaaattgtt cagataattc
     2461 caacgcgatt cccacaaaat ctttcgcttc ctgcgaatcg gagggttcaa gcattcccac
     2521 tttggcggcg cgggcatagt atcggttgtc ttgttcgtct tgcgaggaat gcaaccccgg
     2581 atcgtccgct gagacaatca ccaatcctcc gttcacgccg atataagcac cggtgaataa
     2641 cggatccgca gcaacattca acccgacgtg tttcattgcc gccaaagctc gtccgccggc
     2701 gaatgaagct ccgatggcgg attccgtcgc cactttttca tttggtgccc attcggaata
     2761 aatttcccga tattgcgcga tattttccag aatttccgta ctgggtgtgc ccggataagc
     2821 ggaggcatac cggactcccg cttcccatgc acctctggca acggcttcgt ttcctgtcat
     2881 taactgcttc aaagttgttc ctcctttgta aaatatacga tatatgttat atcttgcaac
     2941 atttatatga attagaatat cacagattga taccttgtca agatgtgtgt tgtaaccttt
     3001 ttgcagatca ttttccgaat tccttgcgat tttaaaacgg aattggtatg ataaatttat
     3061 attttgcaag aaacaaaatt tgaagggagc caatcgatcc tgtgataaaa aagaattccc
     3121 tggtggacca ggtttacgaa gaattgcgaa acggaattat ttcgcttcga atccctttgg
     3181 ggagccggtt gaacgtcaat gagctgcagg acactttgca ggtcagttgc acaccaatcc
     3241 gggaggcgat caatcgtctg caacaggaag ggttggtcac ttatgaaaat aatgtcggtg
     3301 cgcgtgtgct caacttggat gaacatgatg tcgaagaaat ccaacaattg gcgatgacat
     3361 tgcaccgtga ggcgatccgt ctcgcaatgg aacgggagga tcatgcgata ttgctgtccg
     3421 agttggaaca gcatttatcg gaatatgtcg atgcggaaac ggttcaggat gaggtaatgg
     3481 ctgtgaatca tctggtggga acgttttacc atcattgcgg aaatcgccgt ttggacaaga
     3541 gtatgatttc catacaaggc cagcaactcc tgttaagata tatctttgca acttaccgta
     3601 aggatcggaa aagcgggtcg gattttgccc cgctgattca ggctgttcgt gagtcggatg
     3661 tcgaagcggc ctgccgggcg ttacaacaaa atgccgacgg taacaccccg gtgattctcg
     3721 actatttgcg gaagaggaat gcaaacggtt agaccgatgt tctaaaagcc ccatgagctt
     3781 acgaatcatg gggctttttt tgtatagcga tcaggtgttt ttcacccgga caatgtacat
     3841 accgtcctgc caggtgtcca gataaatgaa gccgcgatca tcgaccacac aatcctcggt
     3901 cgtcgccaac atcggaccgg gaaccggaat ctcatagagc agcttttcgg gattgggcgg
     3961 aatgaaatag gccagttctt tgatatagta cggatcggac acatcataca cacgcatccc
     4021 cgcatgaaaa tagcagcaat aaatgcgatc ggcgttttga tccaaccatg gctttccgct
     4081 catcggttcg tgaatattat gcgggccgaa gggtccctga gctccgtcca atcctgcggt
     4141 attgaaattt cgataaggga agttttccgg gacttccggg taggggaatt ccgcaatcaa
     4201 ttgcggatcg gacggatcgg aaatgtcaat catatgaagg ttgttcatcg gttgcgcacc
     4261 tttgccggga ccctcggaca ggattttctt attgtaaaag gggaaacgtt ccccttcatt
     4321 ggttgccacc aggaagtttc ttcccggcag aggcagacag gtgtgagtgc gcgctccggc
     4381 atatttcccg gaaaaaggtc ccttcagctt taattgaccg attttctttg gacgggtgat
     4441 gtcggaaata tccaaaatga tcagcccttc actgtaatag gagagataag ccagatttcc
     4501 gaccgcgaag ggtggtccgt gcagctgcgg ccaatccatg aattcccaat gttgagcgcc
     4561 gtcgaccgga tattcgcctt ctctgagtcc gtcggtgaat tgtcggggat cccaccaacg
     4621 acccacttcc gtcggatgag tggggtcgat gatatcaaga atccgataga tatttccggt
     4681 gaaacccggg caatccgccg ataaatggac atatcgtccg ccgttataca taaaacggtg
     4741 aactcccatt ccgtctttta cgccggtgtc ccaaaaagaa agaagtttgg gatgaatggg
     4801 atcctgccgg atgtcgaaaa tcatcagacc gcttttgttc ttgtctcccg gcttattgcc
     4861 gtgaagaaac ggaacaccgc cgcctaacgc tccgatcatc aatccgtccg ccacctgaat
     4921 tttcggcgta ttggtagcgg ggaattcttt cgggtcgcag gcttccagcc agttaaccac
     4981 cgtcggatga gccggatcgg ttacgtcgac aatattccag ccgggcccgc ggaaagatcc
     5041 gcaatacatg tagtatttgc cgtccgctgt tttatataat gccatctgaa aagcgtttac
     5101 tccgttcaga ttactgaatc cgacaacctc catgtttttg ataaaacctt cgttgccggt
     5161 gcttttcgca taaaacggta tcgccattaa aaaacctctt ctgctttcga gtctgaaaaa
     5221 tcaattaaat gcgggcatgc ttttgggagg ctttacacat aatgctgctg tcagcttcat
     5281 accgaatgcg aaagtgatga aagccactac caaagctact gcataaacag ccgggaaagc
     5341 tttcagacag acaaggaaga aaacgggtcc caaagagaat ccgaccgcta aaaatgtcat
     5401 cacaacggtc ataatcgctg tcatgacaaa tgtgatggac gcgacccgta aaatatggaa
     5461 aaggagaccg ttctcatttt taacgccgca ctttcgggca aaactgtccc ccatcgcttt
     5521 caaatcgata taggttgcga cagtcaatgc cacggtgaag ccgggaataa aatctttaca
     5581 aaatgcttcc aaacctaaaa cgccgttctg ccattccata aacaacggga taaaaatggc
     5641 gaacatcaga caaaagatgg tgttgaacac caaaccgaat tgatcttttc gaaacatatt
     5701 tttctccgga taaaagaatc atttcctgtt ctctcggatg aaaagtctca tattaaaaaa
     5761 cgattgatca gagaacagga atctgtttca acgcatggta atataccctc g
//
LOCUS       DAOMIV010000061         5771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14089_length_5771_cov_3.449090, whole genome shotgun sequence.
ACCESSION   DAOMIV010000061 DAOMIV010000000
VERSION     DAOMIV010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14089_length_5771_cov_3.449090"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKV59_02440"
     CDS             complement(<1..254)
                     /locus_tag="PKV59_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45641.1"
                     /translation="MNRKLIVILFVLLLPACTNMPQDMKESIYTEAAQTLIIQLTETA
                     AAVTPSETATVIPSSTPTEEPTVTTAPIVIPTIASNVTVAP"
     gene            complement(440..1516)
                     /locus_tag="PKV59_02445"
     CDS             complement(440..1516)
                     /locus_tag="PKV59_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45642.1"
                     /translation="MYLVIDNVVKVFPARGGAKEVRALDGITIEINKGELVTLLGPSG
                     CGKTTTLRMVAGFEFPTSGNIILDGKIINDVPTHKRDMSMVFQSYAIFPHLNVYENIA
                     YGLNVQKMSKEEQKKRIDAVLEMVHLEGYETRAPNQLSGGQQQRVALARALVMEPKVL
                     LMDEPLSNLDTKLREKMRFEIRQLQRQLGFTCLYVTHDQLEAMVLSDKVVVMNNGRIE
                     QIGKPRDIYRYPKTKFVADFIGRANFLEGKIIEKRNDELIIMVEGNPLTINDIHYNEF
                     TIGEEVEIIVRPEMILVRTEEGKYSATIKQAAYLGESIEYILDFNGNELTAHETDPSR
                     LEIIPEGSSVKISFMEDCLHILKK"
     gene            complement(1533..3944)
                     /locus_tag="PKV59_02450"
     CDS             complement(1533..3944)
                     /locus_tag="PKV59_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQP45643.1"
                     /translation="MTVNSDSIPPKTRFQGIKRRIHEFGLIAQDPILMIALIFSIIIL
                     TIFIFFPIFRTIIRGFVSDTGEADFTHFARYLDGTFLQGINTFFKNAGLILQETATYG
                     RTYRLVLSNTIKMGIFSATFGTFLGFIFAYATVRCNIPGKKFVHLLALVPTVSPPFAV
                     AIAMIQMFGRNGLITKTLLGIKFTPGMNDIYGMDGLVIVQTLTFFPIAYLMLESMMER
                     LDASMEEAAETLGASRFYLFRTITLPLLIPGIASSFLLLFVESLADLGNPQFISGNVT
                     VLSSQIYMSVIGEFNYQKASALSFVLLLPTLIVYIVQRYYITRRSYVSVTGKPTVGRV
                     YEKNKTIRIIFATMVYLVCILILLVYITVIASSFVKGWGIDFTPTLSNWTKMFGRGLE
                     AIFDTTFLSLYATPFAALLGMIIAFLIVRKKFAGKEILDFGSNLGGAVPGTIVGMGFI
                     LAFNFNNPGLAVFLIIVCSWFYITCVSKANFQRVKILIPGIILGLIMSCLDDGGFLWG
                     KNGWLTGPVSAESQASTAFGIGLNGIYYLIGAIFILIGFYFLLQKNYRKLGKYSFFIG
                     IYIILNKVILIISKPIRIYGKTQSVRWWKAVCTQFADYIEVPFSLPTFFLGVCLFFIT
                     AIMIYEYHGKFDIFFKTLFMIISIAILFTGDQMKMNGTAYIILFAFLIRSLPASVRSG
                     VAALQQIDGSIEEASNILGGDSQYTFRKVTLPLILPAFMTGLIFSFTRHMTSLSAIIF
                     LVSAKWQIVTASIMSGWEQDGISYAATYSSVIIFIVLIFIGLMGVVTRKVLKIDTNID
                     LNAGF"
     gene            complement(4052..5104)
                     /locus_tag="PKV59_02455"
     CDS             complement(4052..5104)
                     /locus_tag="PKV59_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45644.1"
                     /translation="MKRSLIFTFALLILAIAAPVFAQSGELNVLCTPQELWCQGMEEA
                     FEAKYPEIDVQWIRLSSGEGLARVQSEAGNPTFDIWWGGPMDTFIAAKPLGLLEQYES
                     PNLKNIIPEYYDLFVDKDHYYTGIYMGSLGFITNTDWLAENPGVEAPTSFEDLLKPEF
                     KGQIVISHPSTAGTAYTFLSTVLQVMGEEKGWEYLKKFNEQVAIYTKSGSGAGKYVAN
                     GEFAVAIMFSHDGVKEIEDNGAHLVITFPKEGTGYEVGAEAILKGAKNLENTKLWYDW
                     ALTPEAQSLGPKFQSYQAPTVTGVELSHPELLQVKLIDYDFQWSGAHKQEFQDKFANE
                     IQNADMDESGVLIKQP"
     gene            complement(5371..>5771)
                     /gene="mscL"
                     /locus_tag="PKV59_02460"
     CDS             complement(5371..>5771)
                     /gene="mscL"
                     /locus_tag="PKV59_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HQP45645.1"
                     /translation="KGNVIGLAIGVIMGTAFSAIVNSLINDVLMPFIGLLTSGVDFKS
                     WFIPLNGQSYASLKEAQEAAAPVITIGNFIAAVINFLIIAVILFFIMKAMAKAMHTEK
                     KEEPAPVITEPSEEIKLLTEIRDALVKNNG"
BASE COUNT         1802 a         1213 c         1124 g         1632 t
ORIGIN      
        1 ggagcaacgg taacattcga agcgattgtc ggaatgacaa tcggtgccgt cgtcacagtc
       61 ggttcttcgg tcggagtcga tgatggaatg accgtagctg tttcactggg tgtaacggca
      121 gctgccgttt cagtcaattg aatgatcaaa gtttgagcag cttcggtgta aatagattct
      181 ttcatgtcct gtggcatatt tgtacatgcc ggaagtaaca agacaaaaag aatgacgatc
      241 agttttcgat tcataatatc cgttccattt ttttcaatgt acccattata ttcgaaaata
      301 ggattcataa ccattttaga gaaaacgatc gtaaaaaaac ggattattga ataggatcga
      361 aaaagcgcaa attaaataac aaagacgggt tatgcattct tagtgcctta atccgtcttg
      421 aaaattcaaa aaaataccct tattttttta agatatgtaa acaatcttcc atgaaagaaa
      481 tttttaccga agaaccttcc ggtattattt caagtcgtga ggggtcggtc tcatgagcgg
      541 ttaattcgtt tccattaaaa tcaaggatat attcaattga ttcccccaaa tatgcggctt
      601 gtttaatagt tgccgaatac tttccttcct ctgtgcgaac tagaatcatt tccggacgaa
      661 cgattatttc cacttcttcc ccgatcgtaa attcattata atgaatatcg ttaatcgtca
      721 gcgggtttcc ttcaaccata ataatcaatt catcatttct tttttcaatg atttttcctt
      781 ccaagaagtt tgcccgaccg atgaaatcgg caacgaattt tgtcttggga taacgataga
      841 tatcacgcgg tttgccgatc tgctcaattc ggccgttgtt catcacgaca actttatccg
      901 aaagcaccat tgcttccaat tggtcatgag taacataaag acaagtgaaa ccaagttgcc
      961 gttgtaactg tcgaatttca aaacgcattt tctcacgaag ttttgtatcc aagttggaca
     1021 aaggttcatc catgagaagg actttaggct ccataactaa cgcccgcgct aatgccactc
     1081 tttgttgttg tcctccggat agctgattcg gtgcacgggt ttcatatcct tccaagtgaa
     1141 ccatttcgag aaccgcatcg attctttttt tctgttcctc tttgctcatt ttttgaacat
     1201 tcaatccgta agcaatattc tcataaacat tcagatgagg aaatattgcg taactttgga
     1261 agaccattga catatctcgc ttatgggtcg ggacatcatt aataattttc ccgtcgagaa
     1321 taatatttcc ggaagttgga aattcaaaac ccgcgaccat acggagcgtg gtcgtcttac
     1381 cgcaaccgga aggtcccaat aatgtgacca gttctccttt attaatctcg attgtaatcc
     1441 cgtcaagtgc cctaacttct ttcgcaccac cccttgcagg aaatacttta actacgttat
     1501 caattactaa gtacataatc ttctctccct attcagaagc ctgcgttcag atcaatgttg
     1561 gtatctattt tcaaaacttt tcgggttaca acgcccatca atccgataaa tatcaaaacg
     1621 ataaaaatta taacggatga ataggttgcc gcatagctga ttccatcttg ctcccatccg
     1681 ctcatgatgg atgccgtaac aatctgccat tttgcgctta caaggaaaat aattgccgag
     1741 aggcttgtca tgtgtcgggt gaatgagaag atcaaacctg tcataaaggc gggcaagata
     1801 agcggcaaag tcactttccg aaaagtgtat tgactgtctc cgccgagaat attggatgct
     1861 tcttcgatgg atccgtcaat ttgctgcagc gccgcaacac cggaacggac cgatgcgggg
     1921 agagatcgaa tcagaaaggc aaataatata atgtaagcag taccattcat tttcatttga
     1981 tcaccggtga ataaaatggc aattgatata atcatgaaaa gggttttgaa aaaaatatca
     2041 aattttccat ggtattcata gatcatgatt gcagtaatga aaaacaaaca gacgccgagg
     2101 aagaaagtcg gaagagaaaa aggaacttca atgtaatccg caaactgcgt acaaactgct
     2161 ttccaccagc ggacactttg tgtctttccg tatattcgga tgggttttga aatgatcaag
     2221 atcactttat ttaggataat ataaataccg ataaaaaagg aatattttcc taatttacga
     2281 taattcttct gaagcagaaa gtagaatccg atcaaaatga agatggcgcc tatcaaataa
     2341 tagattccgt tcaatccgat cccaaatgcg gtggaagctt gtgattcggc ggaaaccggt
     2401 ccggtaagcc acccattttt tccccataaa aatccgccat catccaaaca gctcatgatt
     2461 aatcccaaaa taatccccgg aatgagaatt tttacccgtt gaaagttggc tttcgataca
     2521 caagtgatat aaaaccatga acaaacgatg atcaggaaaa ctgccaatcc cggattatta
     2581 aagttaaaag ccaaaataaa tcccatcccg acgattgtac cggggacggc accgccgaga
     2641 ttcgagccga aatccaaaat ttctttgccg gcaaattttt tccgaacaat caagaaagcg
     2701 ataatcattc ccaataaagc tgcaaacggg gtcgcgtaca ggctgagaaa ggtggtatcg
     2761 aaaatggctt ccaagccacg tccaaacatt tttgtccaat ttgaaagtgt cggagtaaaa
     2821 tcgattcccc aaccttttac gaatgaactt gcaattacgg tgatataaac aagtaagata
     2881 agaatgcaaa caaggtaaac catggttgcg aagataatgc gaatcgtctt atttttttca
     2941 tagaccctgc caacggtggg ctttccggtt accgaaacat aagaacgacg ggttatgtaa
     3001 tatctttgaa cgatataaac gattaaggtc ggaagcaaca gtacaaaaga gagcgcggaa
     3061 gctttttgat aattaaattc cccgataacc gacatataga tttgcgaaga caaaaccgtg
     3121 acatttcccg aaataaattg agggttgccg agatccgcaa gggattcaac aaaaagtaat
     3181 aaaaagctgg atgctattcc cgggatcaag agcgggagag ttatcgttct gaaaagatag
     3241 aaacggctgg ctcctaatgt ttcagcggct tcttccatcg aagcatccaa tctttccatc
     3301 atcgattcca acattaaata ggctatcggg aaaaatgtca gtgtctgtac aataaccaga
     3361 ccgtccattc cataaatatc attcatgccc ggagtgaatt tgattcccaa taaagtcttt
     3421 gtaatcaagc cgtttctgcc aaacatttgg atcatggcaa ttgcaaccgc aaaaggagga
     3481 gaaaccgtcg gcaccaatgc caatagatga acaaattttt tccccgggat attgcatctc
     3541 acggtggcat aagcaaaaat gaatccgaga aatgttccga aagtggctga gaagatgccc
     3601 atcttaatcg tattggaaag aaccaagcga tatgttcgtc cgtaagttgc cgtttcctgc
     3661 aaaatcaagc ccgcattttt aaagaaagta tttattccct gtaaaaaagt tccgtctaaa
     3721 tatctggcaa aatgagtaaa atctgcttcg ccggtatcgg aaacaaatcc tcttataatc
     3781 gtcctaaaaa taggaaagaa aataaatata gtcaaaatga tgatcgaaaa aatcagagca
     3841 atcatcaata tcggatcttg ggcaattaat ccgaattcat gaatcctacg cttgatccct
     3901 tgaaatctgg ttttgggggg aatactatca gaatttacgg tcatactcaa ctccgattga
     3961 gaaatttata aatattaaag aataaaaaaa tgttacaaaa atgggcggtt ttcttccgcc
     4021 catttttatg acaaaaatct taatcaagaa cctatggttg cttaattaat acgccgcttt
     4081 catccatatc ggcattttga atttcattgg caaatttgtc ctgaaattcc tgcttatgtg
     4141 cgccagacca ttgaaaatca taatcgatca gttttacctg cagtaattcc ggatgggaaa
     4201 gttcgacacc ggtaacagtc ggtgcctgat aggattggaa ttttggcccc aaagactgcg
     4261 cttcgggagt cagtgcccaa tcataccaaa gtttcgtatt ctcaaggttc ttcgcgcctt
     4321 taagaatggc ttccgcaccc acttcatatc ccgttccttc tttcgggaaa gtaatcacga
     4381 gatgagcgcc gttgtcttca atttctttaa caccgtcatg tgagaacata atagcaacag
     4441 caaattcgcc attcgcaaca tattttccgg ctccggaacc tgactttgta taaatcgcta
     4501 cctgttcatt aaatttctta agatattccc aacctttttc ttcgcccatc acctgcaaaa
     4561 ccgttgaaag gaaggtatat gcggttcccg cagtggaagg atgcgaaata acaatctgac
     4621 ctttaaattc cggtttcagc aaatcttcaa aagaagttgg tgcttccaca cccggattct
     4681 cggcaagcca atccgtattg gtaatgaaac cgagtgaacc catatagatc ccggtgtaat
     4741 aatggtcttt gtcaacaaaa agatcataat attcgggaat aatgtttttc aaattgggag
     4801 attcatattg ctcaagcaac cccagaggtt tggctgcaat aaaagtatcc attgggccac
     4861 cccaccaaat atcgaaggta ggattaccgg cttcagactg aactctggct aaaccttcac
     4921 cggatgataa gcggatccac tgaacatcga tctccggata ttttgcttcg aaggcttcct
     4981 ccatcccttg gcaccataac tcttgcggtg tgcaaaggac atttaattca ccactctggg
     5041 cgaaaaccgg tgcggcaatt gccaatatga gcagagcaaa agtaaagatt agactacgtt
     5101 tcattgtttt tcctcctggt agaatgttaa atgttgtttg aactgataat cccgaaaaac
     5161 acagaagaaa ttatttgatt tagatgaata ctttatatca aaatatttac ttctttataa
     5221 agagtatacc tttaaaatct gattttatcg tttatgattt tcatcctgtt tttctgtgag
     5281 attgattatc gtaatcaacg ttcaggaatc ggattaactg agggaataat tgaaaaaaat
     5341 ccggaagacg aaaagggaaa gcaaacagat ttatccgtta ttctttacca gtgcgtctct
     5401 gatttcggta agcagtttga tttcttccga aggttccgtg ataacagggg cgggctcttc
     5461 ctttttctcg gtatgcatcg ctttagccat tgctttcatg ataaagaaaa gaatgacagc
     5521 aataatcaga aaattaataa ccgccgcgat gaaatttccg attgtgatca caggagcagc
     5581 tgcttcctgc gcctccttaa gcgaagcata cgattgtcca ttcagcggta tgaaccagct
     5641 tttaaaatcg accccggaag tcagcaaacc gataaagggc atcagaacat cgttaattaa
     5701 tgaattaacg atggcgctga atgctgtacc catgattacg ccgatagcca gaccgattac
     5761 attgcctttg g
//
LOCUS       DAOMIV010000062         5770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14091_length_5770_cov_4.406824, whole genome shotgun sequence.
ACCESSION   DAOMIV010000062 DAOMIV010000000
VERSION     DAOMIV010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14091_length_5770_cov_4.406824"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="PKV59_02465"
     CDS             complement(<1..689)
                     /locus_tag="PKV59_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQP45646.1"
                     /translation="MGNFIGCDLGGTNLRAAIVNSQTGTVSNLTIVPTLAREGYEEVL
                     LRMVDLFEHLIQESGLGKMNIDGIGIGIPGMMDVERGYTTFVTNLPGHWINIPVGPFI
                     TERIGIPTFILNDVRSITWGEYEFGAGKGCNSMVMYAIGTGIGGGVVINKQLVLGNSG
                     QAGEIGHMTVEPNGTLCNCGNHGCLEQYASGPAIRAAALKAIAHGATTILGEMVEYDL
                     NKMTPEVVFNAA"
     gene            complement(790..1770)
                     /locus_tag="PKV59_02470"
     CDS             complement(790..1770)
                     /locus_tag="PKV59_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQP45647.1"
                     /translation="MSIIPIILAAGQGTRMKSATPKVLHPVMGKPMIDYILDEVAKVT
                     SLRPIVVAGHGADQVIRAVEGRADIAVQQEQLGTANAVAAAEKDAKGRAENVLVAYAD
                     MPCLRAETIRRVCDLQQQSAAIFTMLTIIQEDSHGFGRIVRNADGSVAAIVEEAQASP
                     QQLAIKECNVGLYCFQSEWLWESLKRVKLSPKGEYYLTDLVELANRDSKTVQAVVLED
                     REEALGVNTRVHLSEAEAIMRKRVNTYWMTEGVTMIDPLTTYIDHSVRLERDVTLYPD
                     TWISGETVIRSGSRIGPCTLIENSTIGRDCVICRAAVRNRQLEDHTEITG"
     gene            complement(1912..3216)
                     /gene="folP"
                     /locus_tag="PKV59_02475"
     CDS             complement(1912..3216)
                     /gene="folP"
                     /locus_tag="PKV59_02475"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQP45648.1"
                     /translation="METHRVFIGAGSNLGDRMENLTLAGQLLPPALSILRTSPVYQTQ
                     PWGYSDQPDFYNLVWEAQTTLPPQSLLSYLKGIERKMGREETFRYGPRVIDLDILLFD
                     DLCYQSDNLTIPHPQMIKRRFVLQPLCELIPDEVDPQTGKDWRTLLAEAPEENVRKLP
                     FSLDLSRKVIRWGVKTYLMGIINLTPDSFSGDGLLQGAGDPLESALRKASAFLEEGAD
                     ILDVGAESTRPGFQPVTAEAEQGRLVPVIRRLRERFPDALLSVDTSKAAVAKAALDAG
                     ANWINDVSGGLKDATMTRVAAETNAPIILMRWQPLSEKTETMPQVLREAESLATHALS
                     AGVPHNRIILDPGIGFGTTPQQNLDILRDLAAFKKLGYPILLGTSRKSFIGHYLQKEV
                     DRRLMGTAATVTAGIVSGVDIIRIHDVQYLADVIRMTDLICR"
     gene            complement(3228..3962)
                     /locus_tag="PKV59_02480"
     CDS             complement(3228..3962)
                     /locus_tag="PKV59_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HQP45649.1"
                     /translation="MNRKTPLIAIVCNQELTNNPAEPRIDEVNTHYTRAILNQGGIPF
                     LIPVEFPLGDISLIFEMFDGLLLIGGEDVAIERFGGLPHESVSVPNLERDEIEIELTR
                     LAMAKDFPLLGICRGTQVMNVAMGGSLITDIPSQCPHALLHQRPKDAPPDLVPEHPVR
                     LLPNTMLHAIYETGSLKVNSYHHQAVDRLADCFRPAAYCEDGISEAYEAPDRRFVAGI
                     QWHPERMQDRPDQRKLFRAFVQACSA"
     gene            complement(4060..4404)
                     /gene="rplS"
                     /locus_tag="PKV59_02485"
     CDS             complement(4060..4404)
                     /gene="rplS"
                     /locus_tag="PKV59_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQP45650.1"
                     /translation="MTDILRSIEAPINPNIPDLMPGDSVSVHVKIKEGNRERIQEFKG
                     IVLYTHNNGTNSTFTVRRVASNGIGVERTFLTRSPRIDKVVVERHSKVRRARLYYLRD
                     RSGKSARLKQKF"
     gene            complement(4537..5166)
                     /locus_tag="PKV59_02490"
     CDS             complement(4537..5166)
                     /locus_tag="PKV59_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HQP45651.1"
                     /translation="MKRSETFSPRSKKSFQRPVNHKEVHQRILIICEGTKTEPQYFKQ
                     FRVPGLKIQVEGTGKNTVSLVEHALQYRKDHEEENYDQVWCVFDKDDFPVADFEKAIR
                     LARKNKMCVAYSNQAFELWYILHFEYLNTSMDRKSYIKKLSDYLETEYKKNDDRMYHR
                     LRCKSDIAIKNAERLMKEYHPSHPGSNDPSTTVYELVKVLLNDSKLSSN"
     gene            complement(5156..>5770)
                     /locus_tag="PKV59_02495"
     CDS             complement(5156..>5770)
                     /locus_tag="PKV59_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP45652.1"
                     /translation="GIDNIIIEKAKISESRMFPKDLPQELKDFFLKQIKEGDEMINFR
                     PIHRKLCENGAFEETKFDLTDESEGTQKLFYLSGPIIDTLIGGKVLFIDEIEARLHPL
                     LTRKLISLFHSKELNPHHAQLIFATHDTNLLSNKLFRRDQIWFVEKDEEGASHLYSLA
                     ELKVRNDASFGSEYLQGRYGAIPIFGEFKETLMDLEREDHQDEA"
BASE COUNT         1387 a         1529 c         1335 g         1519 t
ORIGIN      
        1 gcagcgttga agactacttc cggcgtcatc ttgttcaaat cgtactcgac catctctccc
       61 aaaatcgtcg tagcgccgtg tgcgatcgct ttcaaagcgg cggctcgaat ggcgggaccg
      121 gatgcatact gttccaagca tccatggttc ccgcagttgc agagcgtgcc gttgggttca
      181 acagtcatat gaccgatttc cccggcctgt ccggaattcc ccagcaccaa ttgtttattg
      241 atcaccacac cgccgccgat gccggtaccg atcgcataca tcaccatgga attgcagcct
      301 ttgcccgcgc cgaattcgta ttccccccag gtgatcgaac gcacatcgtt gaggatgaaa
      361 gtgggaattc cgattcgttc ggtgataaag ggaccgaccg ggatattgat ccaatgcccc
      421 ggcaggttgg tcacaaaagt ggtatatccg cgctcgacat ccatcattcc gggaataccg
      481 atcccgatgc cgtcgatatt catctttccc aatccggatt cttggatcag gtgttcaaac
      541 agatcgacca tccgcaacaa gacctcttca taaccttctc tggcgagtgt agggacgatt
      601 gtcaggttac tcaccgtccc cgtttgcgaa ttgacgatgg cagcccgcag atttgtgccc
      661 cctaaatcac aaccgatgaa attccccata atatcctccg gaagtatttg tttacagatt
      721 gattaatgat gattctaccc gaaacttcgt ttttgatcgg atgcggactc gtttcgggga
      781 aaaagtcgtt cagccggtga tttcggtatg atcctccaac tgccggttgc gaacagccgc
      841 ccggcagata acgcaatcgc ggccgattgt gctgttttcg atcaaagtgc agggtccgat
      901 gcggctgccg gaacgaatta ccgtttcccc gctgatccac gtatcggggt agagggtcac
      961 atcgcgttcc aatcgcacgg aatgatcgat ataagtcgtc agcggatcga tcatggtaac
     1021 gccttccgtc atccaatagg tattcacccg tttgcgcata atcgcttccg cttcggaaag
     1081 gtggacgcgg gtattcactc ccaaagcctc ttcccgatcc tccaacacca cagcttgaac
     1141 cgtcttgctg tcccggtttg ccaattccac caaatcggtc agataatatt cccctttggg
     1201 ggatagtttg acgcgtttca acgattccca aagccattcg gattgaaagc aatacagccc
     1261 cacattacat tctttgatcg ccaactgctg cggagaagcc tgggcttcct ccacgatggc
     1321 tgccacactt ccgtccgcgt tgcgcacgat tcggccgaaa ccgtgggaat cctcctgaat
     1381 aatagttaac attgtaaata tagccgccga ttgttgttgt aaatcacaga cccgccggat
     1441 cgtctccgcc cgcaggcaag gcatatccgc ataagcgacc aacacatttt ccgcccggcc
     1501 tttggcgtct ttttcggctg cggcgacggc gtttgcggta ccgagctgtt cttgttgaac
     1561 ggcaatatcc gctctgcctt ccacggcccg gatcacctga tccgctccgt gaccggccac
     1621 cacgatcggc cggagagagg taactttggc gacttcatcc aaaatgtaat cgatcatcgg
     1681 ctttcccatg accgggtgaa gcactttggg cgtggcggat ttcatgcggg ttccctgccc
     1741 cgcggccaga ataatcggga taatcgacat agcggctcct ctttttttta taaagttcgc
     1801 atacgggtta tttatgtttg gggtcaaatc cgatttccga tcgttggcga aaatctgatt
     1861 cgcgcggatt tccggcggcc tgctcggttc tccatcctcc gccggcaaac gtcagcggca
     1921 gatcaaatcg gtcatacgga tgacatctgc caggtattgt acatcatgaa ttcggattat
     1981 gtctacgccg ctgacaattc cggctgttac cgttgccgcg gtccccatca ggcgccgatc
     2041 gacctctttt tgcagataat gcccgataaa agattttcgg ctggtgccga gtaaaatggg
     2101 ataacccaat tttttgaaag cggccaaatc tctcaagata tccagattct gctgcggtgt
     2161 cgtcccgaac ccgataccgg ggtccagaat gatccgattg tgggggaccc cggcggaaag
     2221 ggcatgcgtc gccaagcttt ccgcttctct caaaacttgt ggcatagttt ccgtcttttc
     2281 ggagagaggc tgccaacgca tcagaatgat cggtgcgtta gtctcagcgg cgacccgcgt
     2341 cattgtggca tctttcaatc caccgctgac atcgttaatc caatttgcgc cggcgtctaa
     2401 agcggctttc gccactgctg ctttgctggt atcgaccgac aaaagggcat cgggaaaacg
     2461 ctctctcagc ctgcggatga ccggaaccag tcgcccctgt tcggcttccg ccgtaacggg
     2521 ttgaaagccg ggacgggtgc tttccgctcc cacatccaga atatccgcgc cttcttccaa
     2581 aaaagcactc gccttccgca gtgccgattc cagcggatct ccggcgcctt gcaacagccc
     2641 gtcccccgaa aaactgtccg gcgtcaggtt aataatgccc atcaggtacg ttttcactcc
     2701 ccaccggatc acttttcgcg ataaatccaa agaaaaaggc agctttcgga cgttctcctc
     2761 cggcgcctcc gccaacaaag ttcgccaatc tttccccgtt tgcggatcca cttcgtcggg
     2821 aatcagctca cagaggggtt gcagcacgaa acggcgctta atcatctgcg gatggggaat
     2881 cgtcaggtta tcggattgat aacacagatc atcaaacagc agaatatcga gatcgatcac
     2941 gcgcggtccg tagcggaacg tctcttcccg ccccattttc cgttcgatcc ctttcaggta
     3001 ggacaacagg ctttgcggcg gcagcgtcgt ctgcgcttcc cagaccaaat tataaaaatc
     3061 cggctgatcc gaatatcccc aaggttgcgt ttgataaacc ggagaagttc tcagaatcga
     3121 gagggccggc ggtaacaact gacccgcgag cgtcaaattt tccattcgat cgcccaaatt
     3181 acttccggct ccgataaaga ctcgatgcgt ttccatcagg caaactctta cgccgaacag
     3241 gcttgaacaa aagcgcggaa taacttccgt tgatcgggcc ggtcttgcat cctttccggg
     3301 tgccattgaa tgccggcgac aaagcgtcga tccggcgctt cgtaggcttc cgaaattcca
     3361 tcctcgcaat atgcggcggg acgaaaacag tccgccaaac ggtcgaccgc ctgatgatga
     3421 taggaattga ccttgagact gccggtttcg taaatcgcat gcagcattgt attcggcagc
     3481 agcctcaccg gatgctccgg aaccaagtcg ggcggcgcat ccttcggacg ttgatggaga
     3541 agcgcatggg ggcattggga aggaatgtcg gtaatcaacg atccgcccat ggcgacattc
     3601 atcacctgtg tcccgcgaca aatgcccaac agcggaaaat ccttggccat tgccaggcgg
     3661 gtcaactcga tttcgatttc atccctctct aaattgggaa cggatacgct ttcatggggc
     3721 agaccgccga accgttcgat cgccacatct tctccgccga tgagcaacag cccgtcaaac
     3781 atctcaaaaa tcaacgatat gtccccgagg gggaattcga cgggaatcag aaaaggaatt
     3841 ccgccttggt tgagaattgc gcgggtgtaa tgggtattca cctcatcgat tcgcggctcg
     3901 gcggggttgt tcgtgagttc ctgattacac acaatggcta tcagcggggt tttgcggttc
     3961 atgcatccgt cctttcgaaa tcgggtttga ataaaaaaac aggagtgaat ggatccactc
     4021 ctgagggtat cgatttaacg aaaaaatccg gtaaaaagac taaaacttct gttttaagcg
     4081 ggcgctctta ccgctgcgat cacgcaggta atataagcgg gcgcgccgta ctttactgtg
     4141 gcgttcgacg acgactttat cgattctggg ggagcgggtg aggaaagttc gttccacacc
     4201 gatgccgttc gatgccacac ggcggacagt gaaagtggag ttggtcccgt tgttatgagt
     4261 ataaaggaca attcctttaa attcctggat acgttcccgg ttcccttcct taattttcac
     4321 atggacagac acggaatccc ccggcatcaa atcgggaata ttcgggttaa tcggggcttc
     4381 aatgcttctc aaaatatcag tcataattca ataccaatcc ttgcaaaggc tgcttcaatt
     4441 tcagcgccca aacgacgcga agcaagatta tagcatgatc tcccgaacct gtaaagcgga
     4501 ttgtaatatt tcggggacag gtcttttgat gcagccttaa ttcgatgaaa gtttcgaatc
     4561 atttaataaa acttttacga gctcataaac ggttgttgac ggatcattcg atccgggatg
     4621 agaaggatga tattctttca tcaatctttc cgcattttta atggctatat cgcttttaca
     4681 tcgtagccta tgatacatac gatcatcgtt ctttttatat tcggtctcta aataatcact
     4741 caactttttt atatatgatt ttcgatccat tgaggtattt aaatactcga aatgtaaaat
     4801 ataccaaagc tcaaaagcct ggtttgaata ggccacacac attttgtttt tacgtgccaa
     4861 acgaatggct ttttcgaaat ctgcaacagg aaaatcatct ttatcgaaaa cacaccaaac
     4921 ctgatcatag ttctcttctt catgatcttt acgatattgt aaagcgtgtt ctacaagact
     4981 cactgtattt ttcccggtcc cttcgacctg aattttcaat cccggaaccc tgaattgctt
     5041 gaaatattga ggttctgtct tcgttccttc acatataatc agaatccgtt ggtggacttc
     5101 tttgtggtta accggccttt gaaaagattt tttcgaccgc ggagaaaatg tttcactacg
     5161 cttcatcttg atgatcttcc cgttcaagat ccatcaatgt ttctttaaac tcaccgaaaa
     5221 tcgggatggc tccgtatctc ccttgtaaat actcgcttcc gaaagatgca tcatttcgta
     5281 cctttaattc cgccagagaa taaagatggc ttgctccttc ttcatccttt tctacaaacc
     5341 aaatctgatc acgccgaaac aacttgttcg aaagcaggtt tgtatcatga gttgcaaata
     5401 taagctgagc atgatgcgga tttaattctt tggagtgaaa caagctgatt aatttccgcg
     5461 tcagtaaagg atgtaaacgg gcttcgattt catcgataaa caggactttt cctccgatta
     5521 aggtatcaat aatcggtccg gataaataga acaatttttg cgtcccttct gattcatcgg
     5581 tgaggtcaaa tttcgtttcc tcaaaggctc cgttttcgca aagctttcga tgaatcggtc
     5641 taaaattgat catctcatca ccctctttta tctgttttaa gaaaaaatct tttaactctt
     5701 ggggtagatc ctttggaaac attctcgatt cagagatctt tgctttttcg ataataatat
     5761 tatcgattcc
//
LOCUS       DAOMIV010000063         5747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14160_length_5747_cov_4.836261, whole genome shotgun sequence.
ACCESSION   DAOMIV010000063 DAOMIV010000000
VERSION     DAOMIV010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14160_length_5747_cov_4.836261"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PKV59_02500"
     CDS             complement(<1..388)
                     /locus_tag="PKV59_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HQP45653.1"
                     /translation="MSQHPSKIPVTVIVGPTAVGKTAFAIELAKIANAEIVSADSRYF
                     YRGMDIGTAKPTPEERQSIPHHLIDVADPDETWSLALFQQKAYDAIASIHERGKNVIV
                     VGGTGQYIRALLQGWSPPTMEADLVMR"
     gene            complement(369..743)
                     /locus_tag="PKV59_02505"
     CDS             complement(369..743)
                     /locus_tag="PKV59_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQP45654.1"
                     /translation="MVQTNKIELGKIGESIALETAKRRGYQMVATNVRTPYGEIDLIL
                     KTTHQLIFAEVKTRTSFSFGYPEQAITALKLSHMIQSAQFYMLQNNLRDEYRIDVISI
                     LYDSNKHQVANLEWIENVTASE"
     gene            1024..2199
                     /locus_tag="PKV59_02510"
     CDS             1024..2199
                     /locus_tag="PKV59_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQP45655.1"
                     /translation="MREVAILEIGQTPVSEHWDKSLLELAGEPILQIFEKTGISTVDS
                     IFEGNMMTGSANHQLHLGAYIADWVGAGNAAAVHVESACSSGGSAFRAALMAVSSGEA
                     DTAIAVGVEKMTDSPSAEITAELATAADADYERDMGVSFVALNALLMRRYMEVYGWKK
                     EDFAGFSINAHANGAHNPNARFGFPITSEAYMKSPPVCDPINVMDAAGMSDGAAAVLL
                     VPAEKLVKGNKFVTIAGSAAATDTIALQDRRDMLWLSAAERSAKEAYRQAVIGPADID
                     LFEAHDAFTIIEALSLEASGFAERGQGPSLADPAIIGPKGKIPLATMGGLKSRGHPVG
                     ATGIYQIVEAVLQLYGLCGENQVPGAKIAMAQNIGGSGSNVYTHILKINEPRTFQNN"
     gene            2248..>5747
                     /locus_tag="PKV59_02515"
     CDS             2248..>5747
                     /locus_tag="PKV59_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQP45656.1"
                     /translation="MEFHISSQSRIRYQFDSGLFRYDGNVIFADFQAVREFTQKINDV
                     RLKTGNHEPVYAGLVNSLGLIDEIMHYLIDLYRENYVPELNQQIYRWLEEKIGKEALN
                     ETLKAFCEEFPPLNVYQGKITVEEYLDEVNPKGKLNRYEAIEEMLLLWITNQNPAAMK
                     PLGELFDDKELENSTAYNDLISEIQGCFQMLPKVDGMTLVDFLLEPIKQHPDSLFDQL
                     EFIRSRWGTHLGIFLRRLLMSLDWMKEESKAQGVGPGPVEIPVYNDGSLEAGEYEAFS
                     EDRDWMPRVVLIAKNTYVWLDQLSKQYHRPITKLNEIPEEELSTLRDQGFTGLWLIGV
                     WERSKASAKIKQYCGNPEALASAYSIADYRIADDLGGENGFRELKNKAWRYGIRLASD
                     MVPNHMGIDSEWVLYHPDRFLSLPYSPFPSYTFNGPNYSPDPNIGIYIEDHYYTREDA
                     AVVFQRVDYPTGDVRYIYHGNDGTSMPWNDTAQLNYLNPEVRETVIQTILQVAKRFPI
                     IRFDAAMTLAKKHYQRLWFPEPGTGGAIPTRAEFSMTKADFDKAFPVEFWREVVDRVA
                     AEAPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNLLRDEDNAKYRILLKSTLEFDP
                     DILKRYVNFMNNPDEKTAVEQFGKGDKYFGICTMMVTMPGLPMFGHGQIQGFKEKYGM
                     EYRKAYWDEYVDTELLHRHEREIFPLLHQRRRFAEVTNFVFYNFATAYGNVDENVFAY
                     SNYGEGHHSLVVYNNRFGDTKGYIMQSVPFVVKQSDGEKYTTQKMLSESLHLHNDPEC
                     FVVFHDSASGLNYVRPSQQIWREGLYLELYAYQYYVFNDVYEVRDDSEGTWRKLNSWL
                     NGSGTLDLNDTIQELLYHYVLDPYRNCFNGRKLRWLYGKIGLAMTESEWLEIQNGTWP
                     DFEKLMDAFNIFYDLNRSKEGLFNVYCYGINEFLTDPERKMIPGMTRYNAALSKIKKD
                     ITDFPELWIALFISLQQVLLARFERGEVGSGKVENILDDWHLYKAQERILTEAGFTDH
                     LSVSEQINLIQWLYTTLADFQKWDLKTLDDRLNEMLKSDFVRHVLRTNTYQEVLWFNK
                     EAAETFSVLLELIGFYGNIFDPAASASFRIENLMVMQKAAEIFTERVRSSEYHYDRLF
                     VEMPAIENPDRTATSQEKLKT"
BASE COUNT         1564 a         1198 c         1408 g         1577 t
ORIGIN      
        1 atcgcatcac aagatcggct tccatggttg gcggactcca tccttgtaaa agagcccgta
       61 tatattggcc tgtgcctccg acgacaatta cattctttcc ccgttcgtgg atggaggcta
      121 ttgcgtcgta cgctttctgt tggaataaag ccagactcca agtttcatcg ggatcagcga
      181 catcaatcaa atgatgtgga atactttgac gttcttccgg tgtcggtttt gcggtaccga
      241 tatccattcc ccgataaaaa tagcgtgaat ctgccgaaac gatctccgca ttggcaattt
      301 tggctaactc gatcgcaaaa gcggtcttac cgacagcggt gggaccgacg attaccgtaa
      361 cgggaatttt actcggatgc tgtgacattt tctatccatt ccaaattcgc aacctgatgt
      421 ttgttcgaat catacagaat agaaatgaca tcaattctgt attcatcccg aagattattt
      481 tgtaacatat aaaattgggc agattgaatc atgtgagaga gttttaatgc ggtgatcgcc
      541 tgttcgggat atccaaagga aaatgaggtt ctggttttca cctcggcaaa gatcagttga
      601 tgcgttgttt tcaggatcag atcgatttcg ccgtaagggg ttcttacatt tgtcgcaacc
      661 atttgatatc ctctgcgttt tgccgtttcc aacgcaatcg attcaccgat ttttcccagc
      721 tcaattttat tagtctgcac catttttcct ctggtctatt ctatcagagg ttgatctttc
      781 tgaacggctg gccgattacc gtaaaaagca gttctgtaat ttgatcaatg cagagcagaa
      841 aaaataaatt gagctgattt taccgcttcg acagctatca aaccgtcaaa tagcgatttc
      901 cacacttcag ggagatagtg cgtagattat aatttgctta tcggattccg tttaggtcgg
      961 aatactgacc gaacagtttc ctcgaatcga tcaataacag aaaagaaaag gaaatctcct
     1021 tttatgcgcg aagtagcaat tttagaaatc ggtcaaactc ctgtcagtga acattgggat
     1081 aaaagtcttc tggaattggc aggagaaccc atccttcaaa tctttgaaaa gaccggcatt
     1141 tcgactgtcg acagtatttt cgaagggaat atgatgaccg gctccgccaa tcatcagctt
     1201 catttaggag cttatatcgc agactgggtg ggtgccggga atgcggcggc tgttcatgtg
     1261 gaatctgcct gcagctccgg aggatcagcg tttcgggcgg cattgatggc ggtcagttcc
     1321 ggtgaagcgg atacggcgat tgccgtcggt gttgaaaaga tgacggattc cccgtccgct
     1381 gaaattaccg cggaattggc aaccgcagcg gatgccgatt atgaacgaga tatgggcgtc
     1441 tcatttgtgg cattgaatgc cttattgatg cgtcgttata tggaagtata tggttggaag
     1501 aaagaagatt ttgccggatt ttccattaat gcccatgcta acggagcgca taaccccaac
     1561 gctcgtttcg gttttccgat tacatcggag gcatatatga aatccccgcc ggtctgcgat
     1621 ccgattaatg tcatggacgc tgccggaatg agtgacggtg cggcggcggt gctcttagtc
     1681 ccggctgaga agctggtaaa agggaataag tttgttacga tagcgggctc tgccgcagct
     1741 accgatacca tagctcttca agacaggcgg gatatgcttt ggctttccgc tgccgaacgg
     1801 tccgcaaaag aggcttatcg acaggcggta atcggacccg cagatattga tctgtttgaa
     1861 gcgcatgatg cttttacgat tattgaagct ctctcattgg aagcgagcgg ttttgcggag
     1921 cgcggacaag ggccatctct tgcggatccc gcaatcatcg gtccgaaagg caaaataccc
     1981 ctcgcgacta tgggaggatt aaaatctcgc ggtcatccgg tcggagcaac cggaatctat
     2041 caaatcgtcg aggcggtgct gcagttatac ggcttatgcg gggaaaatca agtacccggc
     2101 gcaaaaattg cgatggctca aaatatcgga ggaagcggtt cgaatgttta tactcatatt
     2161 ttgaagatta atgaacctcg aacttttcag aataactaaa tgctatggcg atgactgaca
     2221 tattgccgaa tcagaaagga tgatcacatg gaattccata tatcttctca atccaggatt
     2281 cgttatcagt tcgattcagg tcttttccga tatgacggga atgtaatttt tgctgatttt
     2341 caagcggtcc gagaatttac tcaaaaaatc aacgatgtcc gcctaaaaac ggggaatcac
     2401 gaaccggtat atgccggatt ggtgaattca ctgggattaa tcgatgaaat catgcattat
     2461 ttgattgatc tttatcgtga aaactacgtg ccggaattga atcaacagat ttatcgatgg
     2521 ctggaagaga agataggcaa ggaagctctt aatgaaacat tgaaggcttt ttgcgaagaa
     2581 tttcccccgt tgaatgtata tcagggaaaa atcaccgttg aagagtatct cgatgaagtg
     2641 aatcctaaag gaaaacttaa tcgttatgaa gcgattgaag aaatgctctt gctatggata
     2701 acaaatcaga atccggcggc gatgaagccc ttgggagagt tgtttgatga caaagagttg
     2761 gaaaactcca cggcttataa cgacttaata tcggaaattc aaggttgttt tcaaatgctt
     2821 ccaaaagtgg atggcatgac cttagtggat tttctgctgg aaccgatcaa acagcatccc
     2881 gattctttat ttgatcaact tgagtttatc cgcagccgtt gggggaccca tttgggtatt
     2941 tttctgcgcc ggttgctgat gtctctggat tggatgaaag aagaaagcaa ggcgcaaggt
     3001 gtcggaccgg gacccgttga aattccggtg tataacgacg gatctcttga agcgggggaa
     3061 tatgaagctt tcagcgaaga tcgggactgg atgccgcggg tggtgttgat cgcaaaaaat
     3121 acctatgttt ggctggatca actttccaaa caataccatc gaccgattac caaactgaac
     3181 gaaattccgg aagaagaatt aagcacttta cgcgatcagg gatttaccgg tctgtggtta
     3241 atcggcgttt gggaacgatc caaagcttcc gcaaaaatca aacaatattg cggaaatccg
     3301 gaagcgctcg cttcggctta ctccattgcc gactatcgca ttgcggatga tctcggcggc
     3361 gaaaacggtt ttcgggagtt gaagaataaa gcctggcgct atgggattcg tttggcaagt
     3421 gatatggtac cgaatcatat ggggatcgat tctgagtggg ttttatatca tcccgatcgt
     3481 tttctttctc ttccctacag tccttttcct tcctacactt ttaatggtcc caactattca
     3541 cctgacccga atatcgggat ttatatagaa gaccattatt acacacggga ggatgcggcg
     3601 gttgtttttc aacgcgttga ttatccgacc ggcgatgtcc gttatatcta tcacggaaat
     3661 gacggcacca gcatgccgtg gaatgataca gcccaactga attatctgaa tccggaagtg
     3721 cgggaaacag ttattcaaac cattttgcag gtcgccaaac ggtttccgat tatccgtttt
     3781 gatgccgcca tgacattggc aaagaaacat tatcaacgtt tatggtttcc cgaaccgggg
     3841 acgggtgggg ctattccgac ccgtgccgaa ttcagcatga ccaaagctga ttttgataaa
     3901 gcgtttcccg tggaattctg gcgggaagtg gtggatcggg ttgcggcgga agctccggac
     3961 acgctgttat tggctgaggc gttctggctg atggaaggtt attttgtccg aaccttgggg
     4021 atgcatcgag tttataacag tgcgtttatg aatctgttac gggatgaaga taatgccaaa
     4081 tatcggattc tcctcaaaag cacattggaa tttgatccgg atattcttaa acgctatgtc
     4141 aattttatga acaatcccga tgagaaaacc gcggtagaac aattcggaaa aggggacaaa
     4201 tattttggga tttgtaccat gatggttacc atgccggggc tgccgatgtt cgggcacggt
     4261 cagattcagg gctttaaaga aaaatacggt atggaatatc gtaaagctta ttgggatgag
     4321 tatgtcgata cggagctgtt gcaccggcat gaacgggaaa tattcccgtt gttacatcag
     4381 cgcaggcgct ttgcggaagt gacgaatttt gttttctata attttgcgac cgcatacggt
     4441 aatgtcgatg aaaatgtctt tgcttattcc aattacggcg aaggtcatca ttcattggta
     4501 gtctataaca atcgctttgg ggataccaaa gggtatatca tgcagtcggt tccttttgtg
     4561 gtcaaacaat ccgacggtga aaaatacacc acccaaaaaa tgctttcgga aagcttgcac
     4621 ctgcataacg atcccgaatg ttttgtcgtg tttcatgatt ccgccagcgg attgaactat
     4681 gttcggcctt ctcaacaaat ctggcgggaa ggactttatc tggaacttta cgcttaccaa
     4741 tactatgtat tcaatgatgt atatgaggta agggatgatt cggaaggcac ctggcgaaaa
     4801 ctgaacagtt ggttgaacgg aagcggcacg ttggatctca acgataccat tcaggaactg
     4861 ctgtatcact atgttttgga tccctatcga aattgtttta acggacgaaa gttacgctgg
     4921 ttatacggta aaatcggctt ggcaatgacc gaatccgaat ggctggaaat tcaaaacggc
     4981 acatggcccg attttgaaaa attgatggat gcattcaata tcttttatga cctgaaccgc
     5041 tccaaagaag gcttgttcaa cgtctattgc tacggtatta atgaatttct caccgacccc
     5101 gaacggaaga tgattcccgg aatgactcgc tataatgccg cattaagcaa aatcaaaaaa
     5161 gatattaccg attttcccga actttggatt gcgcttttca tcagtctgca gcaggtgctg
     5221 ttagcgcgtt ttgagagagg agaagtcgga agcgggaaag tagaaaatat tcttgatgac
     5281 tggcatttat acaaagccca agaaagaatc ctcacggaag ccggctttac cgatcatctt
     5341 tctgtcagcg aacaaatcaa ccttatccag tggttgtata ccactttggc ggattttcaa
     5401 aaatgggatc tcaaaacact ggatgaccgg ttgaatgaga tgctcaaatc cgactttgtc
     5461 cggcacgtct tacgaaccaa cacttatcag gaagtcttat ggttcaacaa ggaagcggcg
     5521 gaaacatttt cagttttgtt ggagctcatc ggtttttacg gaaatatatt tgatccggct
     5581 gcatcggctt ccttccggat tgaaaacctg atggtaatgc aaaaagctgc ggaaatattt
     5641 acggaacggg tcagatcttc ggaatatcat tatgaccggt tgtttgtcga aatgccggcg
     5701 attgaaaatc cggaccgaac cgcaacctca caagagaagc tgaaaac
//
LOCUS       DAOMIV010000064         5726 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14230_length_5726_cov_4.107036, whole genome shotgun sequence.
ACCESSION   DAOMIV010000064 DAOMIV010000000
VERSION     DAOMIV010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14230_length_5726_cov_4.107036"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PKV59_02520"
     CDS             <1..591
                     /locus_tag="PKV59_02520"
                     /inference="COORDINATES: protein motif:HMM:NF020287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQP45657.1"
                     /translation="TDMIKNIPRETQIVYPKDVGFILLNLAIGPGKRIIEAGTGSGAL
                     TTAFAYAAGSTGKVYSYDSRADFQKLAEENLKRVALDEQVEFITRDIRLGFTEKNVDA
                     LFLDIPDPYNYLAQVRDALKPGGFFGCLLPTANQVILLLEQMNANQFGFTEVCETGIR
                     YYQTEAERFRPVDRMIAHTGFLIFGRPLIKDEDLMV"
     gene            complement(663..1319)
                     /locus_tag="PKV59_02525"
     CDS             complement(663..1319)
                     /locus_tag="PKV59_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQP45658.1"
                     /translation="MEKVVINAEKRDVVGKKVGSLRRQGLIPGVMYGHNEPPFPITMN
                     AREIERVINNLTSSSIVTVKVNGESHQALIREKQKDYIRNQIIHIDFQILSLKEKIRS
                     KIEVKLVGVAPAVKNFNGIVLQEREFIDVEALPADLPERITVDISGLENIGDLIRVGD
                     LDISDAVTVFDDVNDVIVSISGAMAEEAVEEEVTTAEGTEPEVVEKGKKESEEEEEKK
                     "
     gene            1565..2035
                     /gene="bcp"
                     /locus_tag="PKV59_02530"
     CDS             1565..2035
                     /gene="bcp"
                     /locus_tag="PKV59_02530"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HQP45659.1"
                     /translation="MTELKVGMPAPNFTLPDQMGKIHSLSDFRGKKVVLYFYPKDNSA
                     GCTRQAIGFRDLYEEYMRLAVEIIGISKDSEKSHQKFIESHALPFLLLSDPELNVIKA
                     YDVWKEKNLYGKVTMGIVRTTFIIDEDGIIIKIFQKANANTNAKEVLEYLQSIR"
     gene            2166..4445
                     /locus_tag="PKV59_02535"
     CDS             2166..4445
                     /locus_tag="PKV59_02535"
                     /inference="COORDINATES: protein motif:HMM:NF008503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HQP45660.1"
                     /translation="MIELIDKEIIYSETYGFVQKTDERLADFMKESGLKEISELRRNT
                     IAYQILEKHNHSKNPAQMQLVFDSLASHDITYVGIIQSASASGMKEFPVPYTLTNCHN
                     SLCAVGGTINEDDHVFGLSAAKKYGGIYVPANLAVIHQFEREMMAACGSMILGSDSHT
                     RYGALGTLGIGEGGPELVKQLLQKTYDIDAPKVVLVYLENKPNPWVGPQDVAIALIKA
                     VFPTGFVKNKILEFAGPGIQYLPMDFRNGVDVMTTETACLSSIWQTDESVKTYFELHG
                     RPEAYRPLHPENYAYYDSMVHIDLSKVEPMIALPFHPSNGHTIRELLENPGDILRSVE
                     TESEKIFGKASILKLTDKIHDGRILADQAIIAGCAGGMLDNLCAAADILDHGIVGNDY
                     FSMSVYPASMPINAALTENGVMLRLQKSGVVTKMPFCGPCFGAGDIPANNGFSIRHTT
                     RNFPNRDGSKPDQGQVTGVALMDARSIAATAANRGYITSAADMDIEYKSYPYHFDSEI
                     YRKRVYCGYKKPQKDVELRYSPNITPWPEMYPLAENLLLVLAAVIRDPVTTTDELIPS
                     GETSSYRSNPVKLADFTLSRRVPEYVNRAKKIRDLESERRKGSKLPKFEEILDKLNHP
                     NGANNLNTAIGSVIFANKPGDGSAREQAASSQKVLGGLANIAYEYATKRYRSNCINWG
                     IIPFTIDPEEPFEYQPGDKIFIPGIRKAIAGGIESIPAKIITGSGIKDILLHLKGLTR
                     EEREIIISGSLINFYARKK"
     gene            4477..5676
                     /locus_tag="PKV59_02540"
     CDS             4477..5676
                     /locus_tag="PKV59_02540"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006156.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQP45661.1"
                     /translation="MEKIQMKNPIVELDGDEMTRVIWKWIKDELILPYVDLKVEYYDL
                     GLPARDETDDRITVDAANAIKQYGVGVKCATITPNEKRMEEYKLKKMWKSPNATIRAI
                     LDGTVFRTPILIESIKPVVRTWKKPITIARHAYGDVYKGTEFRIPGKGKAELLFTAED
                     GSEMRDTIYDFESPGVLQGIYNKDSSISSFAHSCFRYALNSKQNLWFSAKDTISKKYD
                     GTFRVIFEKIYEDNYRRNFEDEGITYFYTLIDDAVARVMRSEGGFIWACKNYDGDVMS
                     DMISTAFGSLAMMTSVLVSPDGNYEFEAAHGTVTRHYYRYLNGEETSTNPTAMIFAWT
                     GALAKRGEMDNIPELQEFAKKLEKATLDTLEEGICTKDLADLYEGVTSHPVSTKDFLR
                     EIQKQLI"
BASE COUNT         1780 a         1106 c         1233 g         1607 t
ORIGIN      
        1 acagatatga ttaaaaacat tcccagggag acacaaattg tttatccgaa agatgtcggg
       61 tttatattgc ttaatctggc aatcggtccc ggaaaaagaa tcattgaagc cggtaccggc
      121 tcgggtgctt taacaaccgc ttttgcctat gcggccggtt cgacggggaa agtatattct
      181 tatgacagtc gggctgattt tcaaaagctt gccgaagaaa acctgaaaag agttgccttg
      241 gatgaacaag tcgaatttat aactcgggat attcggttag gatttacaga aaaaaatgtc
      301 gatgctcttt tccttgatat tcccgatcct tataattatt tagcgcaagt cagagatgca
      361 ttaaaaccgg gaggcttttt tggatgtttg ttgccaactg ccaatcaagt aatcctcctt
      421 ttagaacaaa tgaatgcaaa tcagtttgga tttacagaag tctgtgaaac aggaattcga
      481 tattatcaga ccgaagctga acggttcagg ccggttgatc gaatgatcgc tcacacagga
      541 tttctgattt tcggaaggcc tttgattaag gatgaggatt tgatggtgta aaaccaaaac
      601 agaaagcaaa aaaaagacca attcgtcgaa ttggtctttt ttgtatatca tctaaaagga
      661 agctatttct tttcttcctc ttcttccgat tctttcttac ctttttctac aacttcaggc
      721 tccgtacctt ccgctgtcgt tacttcttct tcaacagctt cttcagccat tgcgccggaa
      781 attgaaacaa taacatcatt cacatcatca aaaacggtca cggcatcgga aatatccaaa
      841 tcaccaaccc gaatcaagtc tccgatattc tctaatccgc tgatgtcgac tgtaattctc
      901 tccggaagat cagccggcaa agcttcgaca tcaataaact cacgttcctg taatacaatc
      961 ccgttgaaat tctttaccgc cggagcaact ccgaccaact taacttcaat tttggaacgg
     1021 atcttttctt ttagagataa gatttgaaaa tcaatatgaa tgatttgatt ccgaatataa
     1081 tctttttgtt tttcacgaat taaagcttgg tgtgactcac cattgacctt aaccgtaacg
     1141 atactggagc tcgtcagatt attaatcaca cgctcgattt ctcttgcatt cattgtgatc
     1201 ggaaacgggg gttcattatg cccatacatt acaccgggaa tcaaaccctg tcggcgcagt
     1261 gagccgactt tcttgccaac aacatctcgc ttttcagcat ttattacaac tttttccatg
     1321 attcttcctt cgagtgcctc tcacagatta aacctgttac cttcaccctt ttaaagatat
     1381 tttactcaaa gagctcactg attttagcct tttttatata tacagtcaag ataagtttta
     1441 tgatttaatg atattgttcg aacaaaaaag atgtaaagat attgataaaa aaggcatttc
     1501 gattatattt attggatcgg gtaaaacaaa atgaccgtaa aagaaaaaaa tcagaggggg
     1561 ttttatgaca gaacttaaag ttgggatgcc ggctccgaat tttaccctgc cggatcagat
     1621 gggaaaaatc cattctcttt ccgattttcg cggtaaaaaa gttgttctat atttttaccc
     1681 gaaagacaat tccgcaggat gtacaaggca agctatcggt ttcagagatt tgtatgaaga
     1741 atatatgcgt ttagccgtag aaatcatcgg aatcagcaag gattcagaaa agtctcatca
     1801 aaaatttatt gaaagccatg cgcttccgtt tcttctgctt tcagatcccg aattaaatgt
     1861 tataaaagct tatgatgtat ggaaagaaaa gaacctttat ggcaaggtta ccatgggaat
     1921 cgttcgaact acatttatta tcgatgaaga cgggataatt attaaaattt ttcaaaaagc
     1981 gaatgcgaat acgaatgcaa aggaagttct tgaatatctg caaagtatcc gataaaaaac
     2041 catttgcctc cattgatatg caagtgctta ataatgcttg atcaaagagc tgctggtagc
     2101 tttaaaaata agccgggcat tatttttgtt tatgattcat ttttcatgat ataaggagaa
     2161 attacatgat tgaattgatc gataaggaga tcatttattc cgaaacctat ggtttcgttc
     2221 agaaaaccga tgaaagatta gccgatttta tgaaagagtc cggattaaaa gaaatatctg
     2281 aactccgccg aaatacaatc gcttaccaaa tattggaaaa acacaatcat tcaaaaaatc
     2341 cggcacaaat gcagttagtt tttgattctt tagcatccca cgatattact tatgtcggaa
     2401 ttattcaatc agcgagtgcc agcggaatga aggaatttcc ggttccttat acactgacaa
     2461 attgtcataa cagtttgtgc gcggtcggtg gcaccattaa tgaagacgat catgtatttg
     2521 gactttccgc ggcaaaaaag tacggaggaa tctatgttcc tgccaatctg gctgtcattc
     2581 accaattcga aagagaaatg atggcagctt gcggctcaat gatcttggga tcggatagtc
     2641 atactcgata tggtgcctta ggaaccttgg ggatcggtga aggcggaccg gagctcgtaa
     2701 aacaattgct tcaaaaaacg tatgatatcg atgcgccaaa agttgtattg gtatatttgg
     2761 aaaataagcc gaatccttgg gtcggcccgc aagatgttgc gatcgctctg ataaaagcgg
     2821 tttttcctac cggatttgtc aaaaataaga tattggaatt tgcaggtccc ggaattcagt
     2881 atttaccgat ggatttcaga aacggggttg atgtcatgac aaccgaaacg gcttgtctgt
     2941 catcgatctg gcaaaccgat gaatctgtaa aaacctattt cgaacttcac ggaagacccg
     3001 aagcatatcg accgcttcat cctgagaatt atgcttatta tgattcgatg gttcatatcg
     3061 atctgtcgaa ggttgaaccg atgatcgcat tgcctttcca ccccagcaac ggacatacca
     3121 ttcgcgagtt acttgaaaat cccggtgata tcctccgttc ggtagaaacg gaatcggaaa
     3181 aaatattcgg caaagcatct attctgaagt tgaccgacaa aatacatgac ggtcgtattt
     3241 tagctgatca agcgattatt gccggctgcg cgggtggaat gcttgataat ttatgtgccg
     3301 cagcggacat attggatcac ggaatcgtcg ggaatgatta tttttcgatg agcgtctatc
     3361 cggcaagtat gccgattaac gccgctctaa ccgaaaacgg agtcatgtta cgtttacaaa
     3421 aatcgggtgt ggttacaaaa atgccgtttt gcggaccgtg tttcggagcg ggagatattc
     3481 cggcaaataa cgggttcagt atccgtcata ctacccgtaa ttttcccaac cgtgacggat
     3541 cgaaaccgga tcaggggcag gtgaccggtg tagctctgat ggatgcgcgt tcgattgcgg
     3601 caaccgccgc aaacagaggg tatattactt cggccgcaga tatggatatt gaatataaat
     3661 cctatcccta tcatttcgac agcgagattt accgaaaaag ggtttactgc ggatataaaa
     3721 aaccgcaaaa agatgttgaa ttaagatata gtccgaatat tacgccttgg ccggaaatgt
     3781 atccgttggc tgaaaatctg ctgctcgtat tagccgcagt gatcagagat ccggtgacaa
     3841 caaccgatga attaattcct tcgggagaga cttcttctta tcgatcgaat ccggtaaaat
     3901 tagctgattt cacgctttct cgccgagttc cggaatatgt aaaccgagcc aaaaaaattc
     3961 gcgatcttga atcagagaga cgaaaaggat cgaaattacc gaaatttgaa gaaattttgg
     4021 ataaattaaa ccatccgaac ggcgcaaata acctgaatac cgccatcgga tccgtaattt
     4081 ttgcaaacaa acccggcgac ggatctgcca gagaacaagc tgcatccagc caaaaagttc
     4141 tgggaggatt ggccaatatc gcatacgaat atgcgacgaa gcgatatcgc agcaattgta
     4201 tcaattgggg gatcattcct tttacgatcg atcccgagga accgttcgaa tatcaaccgg
     4261 gagataagat ttttattccg ggtatccgaa aagcaattgc gggaggcatt gaatcgattc
     4321 cagcaaaaat tattaccggt tccggaatta aagatatact gcttcatcta aaagggttaa
     4381 cccgggaaga aagggaaatt attatcagcg gaagcttgat taatttttac gctcgtaaga
     4441 aataaaagaa atcggctgcg aagcatggag tgaaaaatgg aaaagataca aatgaaaaat
     4501 ccgatcgttg aactcgacgg cgatgaaatg actcgggtga tttggaaatg gataaaggat
     4561 gaattgattc tcccttatgt tgacttgaag gttgaatatt atgatttggg tttgccggcc
     4621 cgcgatgaaa cggatgaccg gataaccgtc gatgccgcaa acgccattaa acaatatggg
     4681 gtaggtgtga aatgtgccac aattactccg aatgaaaaaa ggatggaaga atataagctt
     4741 aaaaaaatgt ggaaaagtcc aaatgcgact attcgggcta ttttggacgg aactgtattt
     4801 cgaacaccca ttctaatcga atcgatcaag ccggttgttc gaacttggaa aaaaccgata
     4861 actatcgctc gccatgcata cggtgatgtc tataaaggga cggaattcag aattccgggg
     4921 aagggaaaag cggagttatt atttaccgct gaagacggaa gtgaaatgcg ggatacgatt
     4981 tacgatttcg aaagccccgg ggttttacaa ggaatctata ataaagactc atcgatctcc
     5041 tcttttgcac attcctgttt tcgatatgcg ttaaactcta aacaaaattt gtggttttcc
     5101 gcaaaagata cgatttccaa aaaatatgac ggaacttttc gagttatatt tgaaaaaata
     5161 tatgaggata attatcgacg taactttgaa gatgaaggga tcacttattt ttatacactt
     5221 atcgatgatg ctgttgctcg ggttatgcga tccgagggcg gatttatctg ggcatgcaag
     5281 aattatgacg gcgatgtaat gagcgacatg atttccaccg cttttggatc attggcgatg
     5341 atgacaagcg ttctggtcag tccggatggg aattatgaat ttgaagcggc tcatggaacg
     5401 gttacccgtc actattatcg ctatctaaac ggtgaagaaa cctctacgaa tccgaccgct
     5461 atgatttttg cgtggaccgg agcacttgct aaacgaggag aaatggataa tattccggag
     5521 cttcaagaat ttgcaaaaaa acttgaaaaa gcgaccttag acactttgga agaaggaata
     5581 tgtaccaaag atctcgctga tttatacgaa ggagttactt cacatcccgt atctacaaag
     5641 gactttttac gggaaattca aaaacagctg atttaatatc atgaattttg ttgcgtgatg
     5701 gattaaaaag gctataataa gtaagg
//
LOCUS       DAOMIV010000065         5712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14273_length_5712_cov_3.983737, whole genome shotgun sequence.
ACCESSION   DAOMIV010000065 DAOMIV010000000
VERSION     DAOMIV010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14273_length_5712_cov_3.983737"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /gene="rplB"
                     /locus_tag="PKV59_02545"
     CDS             complement(<1..664)
                     /gene="rplB"
                     /locus_tag="PKV59_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQP45662.1"
                     /translation="MAVKIYKPITPGLRGMTGYTFEEITKDKPERSLIVIRKNHAGRN
                     SDGRITVRHRGGGERRYIRIVDMRRDKIDVPAKVIAIEYDPNRTARIALVQYKDGEKR
                     YILAPIGLKVNDSVVSGPKVEIRTGNAMPISNIPVGSTIHNIEMKPGKGGQLVRAAGS
                     SAQLLAKEGDYAQIRLPSGEVRLILQTCYATIGQLGNLDHSNVKLGKAGRKRHLGIRP
                     TVR"
     gene            complement(664..966)
                     /gene="rplW"
                     /locus_tag="PKV59_02550"
     CDS             complement(664..966)
                     /gene="rplW"
                     /locus_tag="PKV59_02550"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQP45663.1"
                     /translation="MTTDFEILRRPLITEKTNEMAFKLNQYVFEVDPDANRTMVKEAV
                     ESIFKVNVVRVNIIRTPGKSKRNTKNRRLFLKKPVMKKAIVTLAAGDKIPFFEGVE"
     gene            complement(977..1603)
                     /gene="rplD"
                     /locus_tag="PKV59_02555"
     CDS             complement(977..1603)
                     /gene="rplD"
                     /locus_tag="PKV59_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQP45664.1"
                     /translation="MKIDVKNMKGEVVNQIELPETIFAAPIYMDLMHQAYVRQMANAR
                     LGTHETKVRHEVAGGGRKPYKQKGTGRARQGSIRAAQWKGGGRVHTPHMRDYTQDMPK
                     KMRRAALRSALSVKANDSEIVVVDELKLDEPKTRLMANALSLLVGDNSALILIPDKES
                     SVDVIRATNNLPYAKILLANYLNIRDLLRFDRIVIPVAAIETINSFLG"
     gene            complement(1637..2266)
                     /gene="rplC"
                     /locus_tag="PKV59_02560"
     CDS             complement(1637..2266)
                     /gene="rplC"
                     /locus_tag="PKV59_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQP45665.1"
                     /translation="MFKGLLGKKIGMTQIFDENGVAIPITVIEAGPCYVTQLRTQEND
                     GYSAVQLGFEEVKPKRITNAEKGHLKRNNLPPLRFLREFRVKDPQVSEGDKVTVDLFA
                     LGELVDVVGTSKGRGFQGGVKRYNFRGGPKTHGQSDRLRAPGSHGSGTTPGRVFKGSR
                     GPGHMGNDRVTAQNLKVAFIDAERNLIGVKGAVPGARGGLVMIRDARKS"
     gene            2621..3835
                     /locus_tag="PKV59_02565"
     CDS             2621..3835
                     /locus_tag="PKV59_02565"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HQP45666.1"
                     /translation="MKANKNKIRNPFLILLISLIGIGVVSGILLLTHLHTPTVSKEAV
                     VEAMVETEMMQTNTAPTTVGKETTTPTSSQAAAQQTPEAQATTIDPAVYIENEFSREN
                     FETAIRQLSGDIPVTIDGQETRIETRYSYAMFTNQPNAKAEEFLLQTLRQWVPENQIT
                     VEPYVYVDGMGGNTWYNIIVTFPGTVTPQEQILFTAHYDSCVVFEGDPMVSAPGADDN
                     GTGVATLLEAVRIFSTMKFERTLKVIFFSGEENFQQGSKAYAEQHANDNITAVLNMDM
                     FGTDKDGDRCFEMYVGELPGSQKIADLLLQTIQENRLNLKADYLVSNAYAKADQKSFW
                     DRNIPAITVMENFLPDYSPGGCEGVTDRTDFWHLPGDKIEAINLEYAFDIGKAGILTV
                     LKLAGAIPVVPK"
     gene            3883..>5712
                     /locus_tag="PKV59_02570"
     CDS             3883..>5712
                     /locus_tag="PKV59_02570"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HQP45667.1"
                     /translation="MMKLKGFLKEYRYAGLSFLLSLAIFIGLLARIGIFPFGPRTLFM
                     SDMNSIYADFLAEYQRALQGNGSFLYSFHAGLGLNFLAIIAFYLPSPFNWLLAAIPEK
                     NLADGITLITTLKISLSALTFSFFLKKHLPKEDESPLSVAFAVLYSLMGYSVTYLFNM
                     IWLDGVILLPLIFYAIDLIVRDNRWIPAVILYALLFLSNFYIGYMVGIFSALYFLANL
                     FSLQQSDRKVIGKKFLIFTGSVLLAAGLNAVLLIPTGFVLKENMGLFGQAAPGFESKF
                     PLFDLFLKSFPGIFDGYKDSLPQIYCGILTILCLPYYFFNRSIQKREKLCSLLIIILM
                     IFSFNLSPLDFFWHAFDHPSWFPYRYAFLFSFYLLFLGYQGIVRSENTDKRIIFLSAF
                     GMIFYLILVQKMSPTILDDQTFYIGLMFILIDGFILFFGNAKKPSVAWLLVGLCMVET
                     AWNADSTLQRYVPQYSLRTELVDFRERNLSKIRSVLPPEGKFYRVEKTEIRTYNDAML
                     LGYPGIGNFSSVANLSQSTFLKKIGFDCYATWCTYQGRTIFSDSLLGIRFIVSDDPLN
                     YYSNYSEDIYENPYAFPPAFYSQSDFNTFPPLEPDDPISLQNRI"
BASE COUNT         1443 a         1372 c         1235 g         1662 t
ORIGIN      
        1 cgcggacagt cggacgaatc cctaaatgtc gtttccgtcc ggctttgccg agtttaacat
       61 tgctgtgatc caaattgccc agctgcccga ttgttgcgta gcaggtttgc agaatcagac
      121 gaacctcacc ggacggcaga cgaatctgcg cataatcgcc ttcttttgcc agtagttgcg
      181 cggaagagcc ggctgcccga accaattggc cgcctttgcc gggtttcatc tcaatattat
      241 gaatggtgga acccaccgga atattggaga tcggcatggc atttccggtg cggatttcga
      301 ctttcggccc ggagacaacg gaatcattca cctttaaacc gatcggtgcc aaaatatagc
      361 gtttctcgcc atctttatat tgaacgagcg cgatccgagc cgttcgattc ggatcatact
      421 caatcgcaat tactttggcc ggcacatcaa tcttatcgcg ccgcatatcg acaatccgaa
      481 tataacgtcg ctctccgccg ccgcggtgac ggaccgtaat ccggccgtcc gagttgcgac
      541 ctgcgtgatt ttttcgaatc acgatcagcg atcgttccgg tttatctttg gtgatttctt
      601 caaaggtata acctgtcata ccgcggagac cgggggtgat aggtttataa attttaactg
      661 ccattattca actccttcga agaacggaat cttatcaccg gcagccagcg tcacaattgc
      721 ctttttcatg acaggcttct tgagaaataa gcgacggttt ttggtattcc gtttggattt
      781 tccgggcgtt cgaatgatat taacccgaac cacattcact ttaaaaatgg attcaactgc
      841 ttctttcacc atcgtccggt tggcgtcagg atctacttca aaaacatact gattcagttt
      901 gaaagccatt tcatttgttt tttcggtaat taagggacgg cgaaggattt caaaatctgt
      961 tgtcattttc tcctccctat cctaaaaatg aattaattgt ttcgatagcc gccaccggaa
     1021 tgacgattcg atcgaaacgc aacaaatcac gaatatttaa ataatttgcc aacaagatct
     1081 ttgcataagg cagattattg gttgcgcgaa tgacgtcgac gctggattcc ttatcgggaa
     1141 tcagaatcaa tgcgctgtta tcccccacca gcagagataa tgcatttgcc atcaggcgtg
     1201 tcttcggttc atcgagtttt aactcatcaa ccacaacgat ttcggaatcg tttgctttca
     1261 cgcttaacgc agaccgcaaa gccgctcttc gcatcttctt tggcatatcc tgagtgtaat
     1321 cacgcatatg cggagtatga acccgtccgc cgcctttcca ctgagcagcg cggatggaac
     1381 cttgtcgagc ccgaccagtt cccttttgtt tatagggttt cctgccgccg ccggcgactt
     1441 catgccgtac cttggtttca tgtgttccaa gacgagcatt ggccatttgg cgaacatatg
     1501 cctgatgcat caagtccatg tatatcgggg ccgcaaaaat cgtttccgga agttcgatct
     1561 ggttgacgac ttcgcccttc atattcttga catcgatttt cataatactt ctcgctccat
     1621 gttttcggta aacccgttaa gatttacggg catccctgat cataacaaga ccgccgcgtg
     1681 cgccgggaac agcgcctttg acgccgatca gattccgttc cgcgtcgatg aaagcgactt
     1741 tcaggttttg tgccgtaaca cgatcattgc ccatatgacc cgggccacgg gaacccttga
     1801 atacacggcc tggagtcgtt ccggaaccgt gagaaccggg agcgcgtaaa cgatccgact
     1861 gaccgtgggt tttcggtccg ccacggaagt tataccgttt cacaccgcct tgaaaaccac
     1921 gccctttact ggttccgacg acatcaacca gttcgcctaa agcgaacaaa tccacagtaa
     1981 ctttgtctcc ttcggagacc tgtggatctt taacacgaaa ttcacgcaaa aagcgtagcg
     2041 gaggcaagtt gttccgcttt aaatgaccct tttcagcatt cgtgattcgt ttcggtttaa
     2101 cctcttcgaa ccccaactgc accgcgctgt acccatcgtt ttcctgagtg cgcaactggg
     2161 taacatagca aggcccagct tcgatgacgg tgatcggaat tgcaacacca ttctcatcga
     2221 aaatctgggt catgccgatt ttcttgccga gtagcccttt aaacattctc tgttttctcc
     2281 ccttttaagg ttatatataa tagtggaagg tttctaaaaa cctttcctca agattgtcag
     2341 catgggctta gctgcatcat cttgtccaca gtcagtcaat ccgcttctgc cacaccagtg
     2401 gatttttgtc gtgcgcatac ggtcgaattc ttgacgtgcg atatcggttc gctttggcat
     2461 ctatcaaagc aaactgcgac attatatcat aagtttaaaa ttttgtggaa tttgatgtga
     2521 agtcgtaaca aacaaaattt acaatgtaat tttaccctcg aacgccggct atcatatcac
     2581 ggattcacga aacagtataa ttgaattgcc ggagggcttt ttgaaagcga ataagaataa
     2641 aatacgcaac ccttttctga ttttactgat ttctttgatc ggaatcggtg ttgtttccgg
     2701 tatccttctg ttgacacatc tgcatacacc gaccgtgagt aaggaagcgg ttgtcgaagc
     2761 aatggtcgaa acggaaatga tgcagacaaa tacagctccg acaacggtcg gaaaagagac
     2821 aacaacccct acctcaagcc aagccgctgc gcaacaaact ccggaagccc aagcaacaac
     2881 aattgacccg gccgtttata tagagaatga gttttctcgg gaaaatttcg agacggcgat
     2941 ccgccaactc tccggagaca tccctgttac catagacgga caggaaacac ggatcgagac
     3001 tcgttacagc tatgccatgt ttaccaatca acccaacgct aaagcggaag agtttttgct
     3061 ccaaacactc agacaatggg tcccggaaaa tcagataacg gtggaaccct acgtttatgt
     3121 ggacggaatg ggaggcaata cttggtacaa catcatcgtc acattccccg gtacggttac
     3181 gccgcaggaa cagattttgt ttacggcgca ttacgacagt tgcgttgtct tcgaagggga
     3241 cccgatggtc agcgccccgg gagcggatga taacggaacc ggcgtcgcaa cccttctcga
     3301 agcggtacgc attttcagta cgatgaagtt tgagcgtacc ttaaaagtga tttttttcag
     3361 cggagaagag aactttcaac agggcagtaa ggcttacgcg gaacaacatg ccaatgataa
     3421 tattacagcc gttttgaata tggatatgtt cgggacagat aaagatggcg atcgctgctt
     3481 tgaaatgtat gtcggtgaat tacccggctc ccaaaaaatc gccgacctgc tgctgcagac
     3541 gattcaagaa aaccgattga atctgaaagc ggattatttg gtaagtaacg cctatgccaa
     3601 agccgatcaa aaatcatttt gggatcggaa tattccggcg attacggtaa tggagaactt
     3661 tttacccgat tattctccgg gaggttgcga gggagtgacc gatcgaacgg atttttggca
     3721 cctccccggt gacaaaatcg aagcgatcaa tttagaatat gcttttgaca tcggcaaagc
     3781 ggggattctg actgttctaa agcttgccgg agcaataccc gtcgttccca aataatggaa
     3841 tcaattttgc aacatcataa acattaagga caattacccc ctatgatgaa attgaaggga
     3901 tttctaaaag aatatcgtta tgccggtctt tcttttctgt tatccctggc aatatttatc
     3961 ggccttttag caagaatcgg aattttccct ttcggaccgc gcacgctgtt catgagcgat
     4021 atgaattcta tttatgccga ttttttggcg gaatatcaac gagcgcttca agggaacggc
     4081 agctttctat attcttttca tgccggtctc ggtttaaatt ttctggcaat catcgcattc
     4141 tatctgcctt cccctttcaa ctggctgttg gcagccattc cggagaaaaa tttagcggat
     4201 ggaattactc tgattaccac cctgaagatt tccctcagcg ctttaacctt ttcgtttttc
     4261 cttaaaaaac atcttccgaa ggaagatgaa tctccgcttt ccgttgcctt cgctgtcctg
     4321 tactcgctca tgggatattc cgtcacctac ctgtttaata tgatttggct ggatggcgtc
     4381 attcttcttc cgctgatttt ttatgcgatc gatttaattg tccgagataa ccgatggatt
     4441 ccggcagtga tcctttacgc gctgctcttc ctgtcgaatt tttatatcgg atatatggta
     4501 ggaatcttca gcgctcttta ttttctggca aatttattca gcttacagca atcggatcgg
     4561 aaagtaatcg gaaaaaaatt cttgattttt accggttcgg ttctgttggc agcaggtctg
     4621 aatgcggttc tgttgatccc cacgggattt gtgctgaaag aaaatatggg attgtttgga
     4681 caggcagctc ccggctttga atcgaaattt ccgctttttg atcttttcct gaaatcgttc
     4741 ccgggcattt ttgacgggta taaagacagt cttccccaaa tttattgcgg catcctgaca
     4801 atcctctgcc ttccttatta ttttttcaat cgctccattc aaaaacggga aaaactctgt
     4861 tccttgctga tcatcattct gatgatattc agttttaacc tttctccgct cgattttttc
     4921 tggcacgcat tcgatcatcc cagctggttt ccttatcgat atgcattcct tttttctttt
     4981 tatctgctgt ttctgggata tcaaggaatt gtccgctcgg agaatacaga caaaaggatt
     5041 atttttctgt cggctttcgg aatgattttc tatctcattc ttgttcaaaa aatgtcaccg
     5101 accatcctcg atgatcagac attttatatc ggtctgatgt ttattttgat tgatggtttt
     5161 attttgtttt tcggaaatgc gaaaaaacct tcagttgcat ggcttttggt cgggctctgc
     5221 atggtcgaaa cagcttggaa tgccgattcg actctgcaac gatatgttcc tcaatattct
     5281 ctgcgcacgg agttggtcga tttcagggaa cggaatctga gtaaaatccg atctgtcttg
     5341 ccacccgaag gaaaatttta tcgtgtcgaa aaaacagaaa tacgaactta caacgatgcc
     5401 atgcttttag gttacccggg aattggaaat ttcagttcgg tcgccaacct ttcccaaagt
     5461 accttcctga aaaaaatcgg atttgattgt tatgcaacgt ggtgcaccta tcaaggcaga
     5521 acaatatttt ccgactcgtt gctgggaatt cgttttatcg tttcggacga tccattgaat
     5581 tattattcga attacagcga agacatatat gaaaatcctt atgcttttcc gccggctttt
     5641 tattcccaat ccgatttcaa cacttttccc ccattagaac cggatgaccc gatctcatta
     5701 caaaacagaa ta
//
LOCUS       DAOMIV010000066         5691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14345_length_5691_cov_4.329312, whole genome shotgun sequence.
ACCESSION   DAOMIV010000066 DAOMIV010000000
VERSION     DAOMIV010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14345_length_5691_cov_4.329312"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PKV59_02575"
     CDS             complement(<1..867)
                     /locus_tag="PKV59_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQP45668.1"
                     /translation="MSFESFFYPHAVVVNGSMSEGKLGQVLLKKIIDGGYEGKLFAVN
                     PKAQGAFGIQGFPSVASIGEPVDLAVIVSPASTVPSVLDDCGKAGVKAAVVITAGFSE
                     MGNKAGEDEVLEAAKRNHIRVIGPNCAGIVCTSHKFFPTLEIHPPQGGVALVSQSGAL
                     GGVVLAWAQEQGLGISKFVSYGNGIDVNQVELLQYFKDDPETKVVALYIESIRNGREF
                     MEALEACSKIKPVVVIKAGRTNVGQRATASHTGSMAGADAVYDAAFRQCGAVRVKTVE
                     EMMDLCTGFVSLP"
     gene            complement(864..1487)
                     /locus_tag="PKV59_02580"
     CDS             complement(864..1487)
                     /locus_tag="PKV59_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQP45669.1"
                     /translation="MKYDVFMVGVGGQGILSIGEIIASAALTKGIPVNFYPSKGMAQR
                     GGFVKASLRLGGETVGPNIAEKGADLVLSMEVSESLKAIRYVKPGGDFVLLGNIWAPT
                     AVMLGKAGYPSVQDVIDQIKQAGANLVYINEADVPSYDGIKVADNIFMLGIIMKYTKL
                     SQIISVEDIRKVIEERWPKAVEINRYALQCGLDYIPEQIPSVSEGTK"
     gene            complement(1484..3361)
                     /locus_tag="PKV59_02585"
     CDS             complement(1484..3361)
                     /locus_tag="PKV59_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQP45670.1"
                     /translation="MKKLITGNDAIAYGALAAGVKVAVGYPGTPSTGAIAALLDMDLP
                     QTHVEWSTNEKVAIDIAGGVAWAGHRVLCTMKMSGLNVAYDSLISLAYSGCDGGFVIY
                     VADDPGVSAGMCEQDSRGFASMSDMPMLEPTTVEETMHLTKYAFDLSEQIHGPVFIRL
                     TTSLSNAFADAEIIEPAPVSQAEPLLIRDIDRFTKAGAAVCMKQHRDLINRLAKAAEI
                     IDIDRLNSLNLSKKSGGLGIVTGGITIRYLDEALDIAKEYGLDPDSVSILSVKASVPF
                     PKNQAKQLLNHCDSILVLEELEPYIEKELYILARQVGYKGTIVGKLDGTLERIGEYGL
                     SQALIGISKATNIDIPSSVYQSDTKAESIAAARPITVCSGCPHRGTYMAINSAIRKLH
                     LKQKDVMVAGDIGCTILGMNPPFNTVWNEVSMGTSVSLAQGYVYAGVKTPVIATIGDS
                     TFFHGGIPGLINAIQHQVNMTLIIMDNRWTAMTGMQINPGTPPELHHKDYKEIDIAKI
                     VPALGVDQFFEINPFDQDDAVETIVKALSLPGVKVILSRQECAIQAARHKDKNAPQKI
                     VRVIDENCNLCKLCIAVTGCSALVPGKDSMTIDPSLCQQCGLCVGVCNRNALVMEEVS
                     K"
     gene            complement(3422..4537)
                     /gene="hisC"
                     /locus_tag="PKV59_02590"
     CDS             complement(3422..4537)
                     /gene="hisC"
                     /locus_tag="PKV59_02590"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQP45671.1"
                     /translation="MFDVNKIARPCILVRKEYVPGKPVEEVTREYGITDIIKLASNEN
                     PLGASPLAVEAMIKEIRANANYYPESLCPKVAEKLAEKFNLSPNNFYIDNGEDGVITM
                     LGLTFINPGDECIMAELTFPAYENIVGKMDGKSIVIPMTKDYRFDVDGMIRAITPKTK
                     IIFLCNPNNPTGTIIYKDEFERLLAAVPENTLFVCDEAYYEFVDDPNYPQSLNYFNDH
                     PNLIVMRTFSKIMGLASVRIGYAIAHKEIVQVMLKTREPFPVNRVAQAGAFAAMDDKE
                     FCDKTFTVNAAGRKQLFEGLKQMGFEVYAGQANFIFANLGKPSMPVFEGLLKEGVIIR
                     PLAPQGAPNCIRVTIGTPEQNERFLNSFRKVFSEVYP"
     gene            5077..>5691
                     /locus_tag="PKV59_02595"
     CDS             5077..>5691
                     /locus_tag="PKV59_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQP45672.1"
                     /translation="MDFKELESFVEIAKVKSFSTAAKNLFLTQPALSNHIAKLEKELG
                     IALFERNNKKTELTPAGKQFYLSALEILNQRENALLNLEKFQGKIDGMLHIATSSVPG
                     QYILPNILKNFNTKYPDVTYDLHYLSSGEITDMLMMGELDLGFIGAEPDNRKIVYEKI
                     ADDELVVIAPNISPFNKMNTISFERLLDEPLLLRKEGSGTRQAFD"
BASE COUNT         1615 a         1283 c         1126 g         1667 t
ORIGIN      
        1 cggcaaactc acaaatccgg tgcatagatc catcatttct tcaaccgtct tcacccgaac
       61 agctccacat tgtctgaacg cagcatcgta aacggcatcg gcacccgcca tcgatcccgt
      121 atgggaggct gtcgctcgtt gtccgacatt tgtccgaccg gcttttataa cgacgaccgg
      181 tttgatcttg ctgcaggctt cgagcgcttc catgaattcg cgtccgtttc gaatgctttc
      241 aatatataaa gcaaccactt ttgtttccgg atcgtctttg aaatattgga gcagctcaac
      301 ttgattaacg tcaattccgt ttccgtaact gacgaatttc gaaataccca gcccctgttc
      361 ttgcgcccag gctaacacga ctccacccaa agctccgctt tgtgaaacca aagcaactcc
      421 gccttgaggc ggatgaattt caagtgtcgg gaaaaatttg tggctggtac agacaatacc
      481 ggcgcagttc gggccgataa cccggatatg gttccgttta gccgcctcaa gaacctcatc
      541 ttctccggct ttattcccca tttcagagaa tccggcagtg atcacaacag cagcttttac
      601 ccctgctttt ccgcaatcat ccaagacgga cgggaccgtg gaagcgggtg aaacgataac
      661 agccagatca accggttctc cgatagatgc caccgacgga aatccttgga taccgaaagc
      721 gccttgtgct ttcggattta ccgcaaacaa tttcccttca tatcctccgt caattatttt
      781 cttaagcaaa acttgtccca atttaccttc cgacattgag ccgtttacga caaccgcatg
      841 aggataaaaa aaggattcga aactcatttg gtaccctcgg aaacagacgg aatttgttcc
      901 ggaatgtaat ccaaaccaca ttgcaacgca taacggttaa tttcgactgc tttaggccat
      961 ctctcttcaa tcacttttct gatatcttcc accgaaataa tttgtgaaag ctttgtatat
     1021 ttcataatga ttcccagcat gaaaatatta tcagccactt tgatcccatc ataagaagga
     1081 acatctgctt cattgatata gacgagattt gctccggctt gcttgatttg atcgattaca
     1141 tcctgaacag aaggataccc cgcttttccg agcatgactg ctgtcggtgc ccagatatta
     1201 ccgagcaaga caaaatctcc gccgggttta acataacgaa tcgcttttaa agattccgat
     1261 acttccattg ataaaacgag atcggcacct ttttcagcta tattcggtcc tacggtttcc
     1321 cctcctaagc gcagactggc ttttacaaaa ccaccccgct gtgccatccc cttactgggg
     1381 taaaaattta ctggtattcc ttttgtcaaa gctgcgcttg cgataatctc gccgattgac
     1441 agaatgcctt gtccaccgac gccgaccata aaaacatcat atttcatttt gaaacctcct
     1501 ccataaccaa agcgttccga ttgcacactc cgacacaaag cccgcattgc tggcaaagag
     1561 acggatcaat tgtcatagaa tctttccccg gaactaacgc ggaacaaccg gttactgcga
     1621 tacataattt acaaagatta cagttctcat cgattacgcg gacaattttt tgaggagcgt
     1681 ttttatcttt atgacgggca gcctggatag cacattcctg tctcgaaagg attactttta
     1741 ctcccggcaa cgacagtgct ttcactatcg tttcaaccgc atcgtcctga tcaaacggat
     1801 ttatttcgaa gaattgatct acacctaaag ccgggacgat ttttgcaata tctatttctt
     1861 tataatcctt atgatgaagt tcgggcggtg ttccgggatt gatttgcatt cccgtcattg
     1921 ctgtccaacg gttatccatg ataattaaag tcatatttac ttggtgttgg atcgcattga
     1981 tcaaaccggg gataccgccg tggaagaagg tggaatcgcc gattgtcgca attacgggag
     2041 tttttactcc ggcataaaca tagccttgtg ccaaactgac actcgtaccc atggatactt
     2101 cattccaaac ggtattaaaa ggaggattca ttcccagaat tgtgcaccca atatcgcctg
     2161 ccaccataac atctttttgt ttcagatgca atttacgaat cgcactgtta atggccatat
     2221 atgtcccgcg atgcgggcaa cctgaacaga cggtaatcgg cctggcagcc gctatcgatt
     2281 cggctttggt atcactctga tagacagaag agggaatatc gatatttgtg gctttcgaaa
     2341 tgccgatcaa cgcctggctc aatccgtact caccgattct ctcgagtgtt ccgtccaatt
     2401 tcccgacaat tgtcccctta tatccgacct gacgggccag aatgtatagt tctttttcga
     2461 tataaggctc taattcttcc aagactaaaa tcgaatcgca atgattcagc aattgctttg
     2521 cctgattttt aggaaacgga acagatgctt tgaccgataa aatcgatacc gaatcgggat
     2581 ccaatccata ctcttttgcg atatctaatg cttcatcaag atatcttatt gtgattccgc
     2641 cggtaacaat ccccaagccg cctgattttt ttgacagatt caaggaattc aaccggtcaa
     2701 tatcgataat ttcagctgct ttggctaatc gattgatcaa atcgcggtgt tgtttcatgc
     2761 ataccgctgc tccggctttg gtgaaacggt cgatatcccg gataaggagc ggttccgctt
     2821 gtgagaccgg tgcgggttcg atgatttccg catcggcaaa tgcatttgac aacgaagtgg
     2881 taaggcggat aaataccggc ccatgaattt gttccgaaag gtcaaaagca tattttgtca
     2941 gatgcatggt ttcttcgacg gtggtcggtt ccagcatcgg catatctgac attgatgcga
     3001 aaccgcgtga atcttgttca cacatccccg cgctgacgcc gggatcatcg gccacataaa
     3061 tcacaaatcc accgtcacat ccggaatatg caagcgaaat taaagagtca taagcaacat
     3121 ttaatccgga cattttcatc gtacataaaa cacgatgacc tgcccaagcg acccctccgg
     3181 cgatatcgat ggcgactttt tcatttgtgc tccattcaac atgagtctga ggcagatcca
     3241 tatccaaaag agctgcaatc gctccggttg acggtgtgcc aggatatcct accgcgactt
     3301 tgactcccgc ggctaaggca ccgtaggcaa tcgcatcatt tccagtaatt agtttcttca
     3361 atccgaacct ccctgttcat ttctaaaata gatgcattaa tgataatttc atggctttaa
     3421 attacggata cacttccgaa aaaacttttc tgaaggaatt cagaaatctc tcgttctgtt
     3481 ccggagttcc gatcgtaaca cggatgcagt tcggtgctcc ttgcggggca agcggtcgga
     3541 taattactcc ctctttgagg agcccttcaa aaaccggcat ggacggtttc cctaaattgg
     3601 cgaaaatgaa gtttgcctga ccggcatata cctcaaaacc catttgcttt aagccttcaa
     3661 aaagctgttt tcttccggcg gcatttactg taaatgtttt atcgcaaaat tctttatcat
     3721 ccatggccgc aaatgcaccg gcttgggcaa cgcggttaac agggaacggt tctctcgttt
     3781 ttaacatgac ctgaacgatc tccttatggg cgattgcata accgatacgg acggaagcga
     3841 gtcccataat tttggaaaaa gtacgcatca cgattaaatt aggatgatcg ttaaaataat
     3901 tgagtgattg ggggtaattc gggtcatcga caaattcata gtaagcttca tcgcaaacaa
     3961 ataaagtatt ctccggcaca gcggccaata aacgctcgaa ttcgtcctta tagataatcg
     4021 ttccggtcgg gttattcgga ttacatagga aaatgatttt cgttttgggt gtaatagccc
     4081 gaatcatccc atcgacatca aagcgataat cctttgtcat cggaatgaca atacttttcc
     4141 cgtccatttt tccgacgata ttttcgtaag ccgggaaagt cagctcggcc atgatgcatt
     4201 cgtctccggg attaataaaa gtgagtccga gcatggtgat tacaccgtct tctccattat
     4261 cgatataaaa attattcgga gaaagattga atttttcagc taacttttct gccactttcg
     4321 gacataaact ttccggataa taatttgcgt ttgcgcgaat ttctttgatc attgcctcaa
     4381 ccgcaagcgg tgaagcgcct aagggatttt cattcgatgc taatttaata atatcggtaa
     4441 tcccatattc acgtgtaact tcttcaaccg gtttaccggg tacatattct ttacgtacaa
     4501 gaatacaagg acgagctatt ttattgacat caaacacggc tttacctcct gaaatataat
     4561 ttcctttatt aatcattttg agaaatcgga taatctcctg tttcccataa agttttataa
     4621 aaaaatttca aaaaaatcga tcgttatcgt aaaatgttct gctataagaa atattcttat
     4681 ccgcaagttc aagctaagaa aacaatattt ccttccttca gtataacatt cttagatatt
     4741 atgaaacaat gaggataatt agttttttct tattacattt atacatgcta tcatgaattt
     4801 tgacaatggt aaaatattgt ttacaacttc ttcaattatg gctatattat tcaaaaagat
     4861 aagtttttta agatagtata ttagtgattt agcaagataa attgatagat gatctaatta
     4921 ctataaaata tcggaatatt gtaatttata taaaatgttt ttggtaaaca gttaagaaaa
     4981 acaaaaataa tttgaatccg ctataattat aagaacataa aattcatacg tgtttttatc
     5041 atacattgtt ttatttattt gaagtgaggt gtcaatatgg attttaaaga gttggaatca
     5101 tttgtggaga ttgcaaaggt taaaagtttt tctactgcgg caaaaaattt atttctgaca
     5161 cagcctgctt taagtaacca tatcgccaaa ttagaaaaag aattggggat cgcattattt
     5221 gaacgaaata acaaaaaaac cgaattgaca cctgcgggga aacagtttta tctttcggca
     5281 ctcgagattt taaaccaacg tgaaaatgca ttgcttaatt tggaaaagtt ccaaggaaaa
     5341 atcgatggca tgctgcatat cgcaacgagc agtgtccccg gtcaatatat ccttccgaat
     5401 attttgaaaa atttcaatac aaaatatccg gatgtaactt atgatttgca ttatctcagt
     5461 tcgggtgaaa taaccgatat gctgatgatg ggtgaattgg atttgggatt tatcggtgcc
     5521 gaaccggata atcgaaagat tgtctatgaa aaaattgcgg atgatgagtt ggtggttatt
     5581 gcgccgaata tcagtccgtt taacaagatg aataccatta gctttgagcg attgttggat
     5641 gagccgttgc tgctgcgaaa agagggatcc ggaacgcggc aggcttttga t
//
LOCUS       DAOMIV010000067         5685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14361_length_5685_cov_3.396625, whole genome shotgun sequence.
ACCESSION   DAOMIV010000067 DAOMIV010000000
VERSION     DAOMIV010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14361_length_5685_cov_3.396625"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..391)
                     /locus_tag="PKV59_02600"
     tRNA            complement(311..391)
                     /locus_tag="PKV59_02600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(355..357),aa:Leu,seq:caa)
     gene            444..1520
                     /gene="ychF"
                     /locus_tag="PKV59_02605"
     CDS             444..1520
                     /gene="ychF"
                     /locus_tag="PKV59_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HQP45673.1"
                     /translation="MRLGIVGLPQSGKTTLFNALTRGSFPVGTATGKIEIHDAVVDVP
                     DERVDVLAKMFHPKKTIYAKVNYADIAGLDGSSAKSGISGELLNKLAQMDGFIHVVRC
                     FTNDQVPHPAGSIDPIRDIQTLDGDFLLNDLISVEKKLERLTDEKRKGGGREKAVIDR
                     EIELFEKLHACLAEEKPLRELDLTAEEEKAISGFGLLSRKPVLILLNLDETQEVPRID
                     YPYKQSKVESIRGQIEMELAQLSPEDAAMFMEDYHISELGLNRVIRLSYELLGQLSFF
                     TAGADECRAWTVRRGATAPEAAGVIHTDLQKGFIRAEVIAYDDLIELGGMNEAKAKGK
                     LRLEGKDYIVKDGDILNIRFNVSK"
     gene            complement(1609..2556)
                     /locus_tag="PKV59_02610"
     CDS             complement(1609..2556)
                     /locus_tag="PKV59_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HQP45674.1"
                     /translation="MRPEISVLSIILIIEAVWWLVWILLALIRKKFSVFAALLGTLLI
                     WGLAGLFLVSSVPEEGPMTGQLVGLVILAIIPFIMLPPMVVIINEYQRGVLFRLGRLM
                     ATLEPGLNIIFPFGIDRVVKIDLRTFTIDVSKQEVITKDNVPVLVDAVVYFNVFDPIL
                     SVTKVANYTLSTTLLGQTSLRSVLGQHELDEILSKRTELNEVLRKLLDEDTDPWGIKI
                     SAVEIKSIELPDTMKRAMAKQAEAERERRAKIIAADGEFQAAQKLLDAASVISKDPAT
                     LQLRYLQTLSEIAVERNSTILFPLPMELLKAFGNKQAPE"
     gene            complement(2599..3981)
                     /gene="lpdA"
                     /locus_tag="PKV59_02615"
     CDS             complement(2599..3981)
                     /gene="lpdA"
                     /locus_tag="PKV59_02615"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HQP45675.1"
                     /translation="MNAYDLIVIGAGPGGYIAAIRGSELGLKTAIVEKDSLGGVCLNV
                     GCIPSKSLLKNAELVRTLRKESETFGIRASDLSFDYAAAVDRSRKVSKRLNQGIAFLM
                     KKNKIDVYNGKASFTDPKHVRVSLNSGEEEFLEAADFIIATGTVPTELKGMEFDGERI
                     LNYRQAILQTKLPNSVVIIGGGAIGVEFATIWSAYGSAVTVVEMQPNLLPNEDPEAGR
                     ELERSFKKQGIDVKTGTRLKSIDKVPDGVMIHLINEKGESEVSAQQVLIAVGFRPNPA
                     ELNLEAAGVKTTERGWIDCDPFGKTAADHIYAVGDITGKILLAHSAMTMGRIAAEQIG
                     GLITEPLNLDLVPHCTFSFPQVASFGLTEASADARKIKIKAGKSAFLANGKALGMNEN
                     EGWIKLICDATDHRILGATMIGAEVCELLPELTLAATKQLTAEDILMNIHAHPTLSEG
                     IAEAAGNLLI"
     gene            4190..5125
                     /locus_tag="PKV59_02620"
     CDS             4190..5125
                     /locus_tag="PKV59_02620"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQP45676.1"
                     /translation="MNIDLTVLQAFESIRTPALDAFFLLVTQFGEAAILIPALCIIYW
                     CFNKKQAFFLALNFFSALILNHILKITFCIERPWLRWQGEIAPVAKAKITATGYSFPS
                     GHVATATSVYGGIALWYRKNKKIRIAAIVLILAVSLSRIYLGVHTPSDVLASIFAGLI
                     LLFLNLRIFNRITANPEDDKKFLLVSLFVLILGLIYTFYKSHWNPSMEILWLDGIETM
                     GTAAGVLTGWYWEEHRIRFQPIRRVGPAMIVVTIGLAGTFLILSVIGKFLKAIFGILS
                     GNFLCYFLIGFWIIGLYPYLFNKILNPVSDEKYVH"
     gene            5115..>5685
                     /locus_tag="PKV59_02625"
     CDS             5115..>5685
                     /locus_tag="PKV59_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQP45677.1"
                     /translation="MYTDSGLRIRKIKTRKDVLQIGELIENCFHPYLDPDGRRFVENL
                     QQIGKEYRDHPFFSLFRSFDLLLKGFVSINPEGKIDGVVTLFPCNVEYSFGYLIANVC
                     VAPESRHKGIATALMAFALRYAEKHHADTVYLQVREETPEAVRLYRKLNFTEDAYRTI
                     WIRPKSDMQYPDEKVITFTEPNEQERTDFI"
BASE COUNT         1510 a         1253 c         1288 g         1634 t
ORIGIN      
        1 aagtatagca tattcacttc gtaattcgaa tgttaaggaa gagcctgata ttcggtacat
       61 accaatcgga aaaaaacagg ggacgtcttt tgggaaatat gaattaatcc gattgaaaat
      121 ccgaaaatcc gcaatagaaa attcgttcaa gcggagagca tttccggttg tgtgatcttg
      181 actttattct gtaccgactt ttatttcgac agttaacatc cgttggctca attttagcat
      241 attacggtaa ggagttcgga acaaaaaccc tccgtcgaat ttttcgacgg agggtatgtt
      301 ataatttttg tgccgaaggt gggaatcgaa cccacataca caaggtacac gattttgagt
      361 cgtgcgcgtc tgccagttcc gccacttcgg ctgaagttct tcaattctac ccacaaaatt
      421 aactgtggtc aaggagatct tacatgcgac ttggaattgt cggtctgcct caaagtggaa
      481 aaaccacttt atttaatgca ttaacccggg gcagttttcc ggtaggaacc gctacgggga
      541 aaatagaaat tcatgatgcg gtcgttgatg tccccgatga gagagtcgac gtactggcaa
      601 agatgtttca tccgaaaaag acaatttatg ccaaagtgaa ctatgcggat atcgccggat
      661 tggacggaag ctccgccaag agcggaatat ccggtgaatt gttaaacaaa ttggcacaaa
      721 tggacggatt tattcatgtc gtacgttgtt ttaccaatga tcaggtacct catcccgccg
      781 gaagtattga tccgatacga gatattcaga cattggacgg ggatttttta ttaaacgatc
      841 tgatttccgt cgaaaagaaa ctggaacgat tgacggatga gaaaagaaaa ggcggcggca
      901 gggaaaaagc agtcatcgat cgagagattg aattattcga aaaattacat gcctgcctgg
      961 ccgaggaaaa gcctttacgg gaattggatc tgaccgccga agaagaaaaa gcgatttccg
     1021 gtttcggatt actgtcacga aaaccggtat tgattctttt gaatttggat gaaacccagg
     1081 aagttccgcg gattgattat ccttataaac agagcaaagt cgaatcgatt cggggacaaa
     1141 tcgaaatgga attagcccag ctttcccctg aggatgccgc gatgtttatg gaagattatc
     1201 atatttccga attgggactg aatcgggtga tcaggctttc ctatgaatta ttggggcaat
     1261 tgtctttttt taccgccgga gcggatgagt gccgagcgtg gacggtacgc cgcggggcta
     1321 cggcgccgga agccgcaggt gtgattcata cggatctgca aaaaggcttt attcgggctg
     1381 aggtcattgc atacgatgat ttaattgaat taggcggcat gaacgaggca aaagccaaag
     1441 ggaaactgag gttggaggga aaagactata tcgtaaaaga cggtgatatt ttgaatatcc
     1501 gttttaacgt ttccaaataa aatcgaacgg gagcaaatga tttaacaaag atccggaaat
     1561 ttttccggat ctttgttatt cttcgagatt aaatcttttg cgctttgctt attcgggagc
     1621 ttgtttatta ccgaacgcct ttaacaattc catcgggagc gggaacagga ttgttgaatt
     1681 cctttcaacc gcgatttcac tgagggtttg caagtagcgc agttgcaagg tcgccggatc
     1741 ctttgagatc acggaagccg cgtccagaag cttttgagcc gcctgaaatt cgccatcggc
     1801 cgcaataatt tttgcccggc gttcgcgctc tgcttccgct tgttttgcca ttgcccgttt
     1861 catggtatcc ggcaattcaa tacttttaat ctcgacagca gaaatcttga ttccccatgg
     1921 atcggtatcc tcgtccaaca gttttcgcaa aacttcattg agttcggttc gtttggaaag
     1981 gatttcatcc agttcgtgtt gccccaatac cgaccgtaag gaggtctgac cgagcaaagt
     2041 ggtgctcaaa gtataattgg caactttcgt aacggataaa atgggatcaa atacgttgaa
     2101 atagactacg gcatccacca ataccggcac gttgtccttg gtaattacct cctgttttga
     2161 gacgtcaatc gtaaacgttc gcaagtctat tttcacaacc cgatcgattc caaacgggaa
     2221 aatgatattc aatccgggtt caagggttgc cattaaccga ccgagccgga aaaggacacc
     2281 tcgctgatat tcgtttatga tcaccaccat cggcggcagc atgataaacg gaataatggc
     2341 taaaatcacc aatccgacca attggccggt cataggccct tcttcgggga cagaactgac
     2401 caagaaaagc ccggctagtc cccaaatcaa taatgttccc aaaagagcgg cgaaaacaga
     2461 aaactttttt ctgatcagag ccaataaaat ccaaaccagc caccaaaccg cttcgataat
     2521 caaaataatc gataaaactg atatttcagg cctcatttat tgttcctctt tctaaaactc
     2581 agtcaatccc tttcttaatc atatcagtaa attcccggca gcctccgcga ttccttcgct
     2641 taaagtggga tgagcatgga tattcataag aatatcttct gctgtcaatt gttttgtcgc
     2701 cgcaagggtt aattcgggta acaattcgca cacttccgca ccgatcatcg tcgcacccag
     2761 aattcgatga tcggtcgcat cgcaaatcaa tttaatccag ccttcatttt cattcatacc
     2821 gagtgctttt ccgtttgcga ggaaggcgga ttttcccgct ttgattttaa tctttctcgc
     2881 atcagcggaa gcttccgtca atccgaaaga tgccacttgc gggaagctga aggtgcaatg
     2941 cggaaccaaa tccaaattca acggttcggt tataagaccg ccgatttgtt ccgcggcgat
     3001 tcttcccatt gtcatggctg aatgcgccag taagattttc ccggtaatat ctccgacggc
     3061 atagatgtga tccgcagcgg tttttccgaa cggatcgcaa tcaatccatc cccgttcggt
     3121 ggtttttact ccggcggctt ccagattgag ctcggcggga ttcggtcgaa atccgacagc
     3181 aatcaaaacc tgttgcgccg acacttcgct ttcacctttt tcattgatca aatgaatcat
     3241 cacgccgtcg gggactttgt cgattgattt caagcgggtg cctgttttta catcgattcc
     3301 ttgttttttg aaagatcgtt ccaattcacg cccggcttcc ggatcttcat tcggcagtaa
     3361 attcggttgc atttccacga cggtaaccgc ggaaccgtac gcgctccata tcgtggcaaa
     3421 ttctacgccg attgcaccgc cgccgataat tacaacggaa ttcggcaatt tggtttggag
     3481 aatcgcctga cgataattta agattctttc accgtcaaac tccatccctt tcagttcggt
     3541 cggaacagta cccgtagcga taataaaatc ggctgcttca agaaattcct cttctccgga
     3601 attgagcgaa acgcggacgt gtttgggatc cgtaaaagag gctttcccgt tatagacgtc
     3661 gattttattc ttcttcatca gaaaagcgat gccctgattg agcctcttgg aaacttttcg
     3721 gctcctatca actgccgctg cgtaatcaaa agacaagtcg ctcgcacgaa taccgaaggt
     3781 ctcgctttct tttcgcagtg ttctcaccaa ctcggcattc ttgagcaaag attttgaggg
     3841 aatacatccg acattcagac agactcctcc caaactgtct ttctcaacaa tggcggtttt
     3901 cagtccgagt tcagaaccgc gaatcgcggc aatgtatcct ccggggccgg caccgataac
     3961 gatcagatca tatgcattca tttgattttg tcctttgcta atctttttta atatggcatc
     4021 aaatacccgg aatcgggatc tcatgggtat gattataata attttgccgt aaatatgaga
     4081 ttttgacggt tcaggacgac agaattcgct tttttacctc cttaattgta aatttctccg
     4141 attctgttcg ttataattta gaataaattt ttaagagaag agaaccggca tgaatatcga
     4201 tttaaccgtt ttgcaagctt tcgaatccat tcgaactcct gcgcttgacg cctttttttt
     4261 actggtcaca cagtttggag aagcagccat tctgattccg gcattgtgca ttatttactg
     4321 gtgtttcaac aaaaagcagg ctttttttct ggcgcttaat tttttctcgg ctctcattct
     4381 caaccatatt ttaaagatca ctttctgtat cgaacgccca tggctgcggt ggcaggggga
     4441 aattgcgccg gtggcaaaag caaaaatcac agctaccggc tattcatttc ccagcggtca
     4501 cgttgccacg gcaacgagtg tctatggcgg gatcgcactt tggtatcgaa aaaataaaaa
     4561 gattcggatt gcggcaattg ttttgattct cgcggttagt ctgtcacgaa tttatttagg
     4621 tgttcatacc ccctcggatg ttttggcttc gatttttgcc ggattaattc ttctttttct
     4681 gaatttaagg atttttaatc ggattaccgc aaacccggag gatgataaaa agtttttgct
     4741 ggtttcgctt tttgtcctga ttctcgggct tatctatacg ttttataaat ctcattggaa
     4801 tccgtcgatg gaaatcttat ggctggacgg tatcgaaaca atggggaccg ctgccggagt
     4861 tttgacgggc tggtattggg aagagcatcg gattcgtttt cagccgattc gtcgggtggg
     4921 gccggcgatg attgttgtta ccatcgggtt ggcgggaacg tttctgatct tatctgttat
     4981 cggtaaattt ttgaaagcga ttttcggaat tttatcggga aattttttgt gttatttcct
     5041 gataggtttc tggatcatag gactctatcc gtatcttttc aacaagatat tgaatccggt
     5101 ttccgacgaa aaatatgtac actgattccg gactccgtat ccgcaaaata aaaacccgaa
     5161 aagatgtgct ccaaatcggt gaactcatag agaattgttt tcatccctat ttggatccgg
     5221 acggcagacg atttgttgaa aatttgcagc aaatcggaaa agaataccgc gatcatcctt
     5281 ttttcagttt gttccgatct ttcgacctgt tgcttaaagg atttgtcagt ataaatccgg
     5341 agggaaaaat cgacggtgtg gtgacgcttt ttccctgtaa cgtcgaatat tcgttcgggt
     5401 atttaattgc caacgtctgt gtggctccgg aatcacgtca taaaggaatc gcaaccgctt
     5461 tgatggcttt tgcgctccgt tatgctgaaa aacaccatgc cgatacagtc tacctgcagg
     5521 tccgggaaga aacacccgaa gcggttcggc tttatcggaa attaaatttt accgaagatg
     5581 cctatcgaac gatttggatt cgaccgaaat cggatatgca atatccggat gaaaaggtaa
     5641 taacgtttac ggaaccgaac gaacaggaac ggactgattt catac
//
LOCUS       DAOMIV010000068         5681 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14370_length_5681_cov_4.200498, whole genome shotgun sequence.
ACCESSION   DAOMIV010000068 DAOMIV010000000
VERSION     DAOMIV010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14370_length_5681_cov_4.200498"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PKV59_02630"
     CDS             complement(<1..576)
                     /locus_tag="PKV59_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HQP45678.1"
                     /translation="MEVFDFSKIKSIFLDMDGVLWNGKNPIGNTRDIFTKLDQADIFP
                     VFITNNSTRTPESYQEKMAGFGVKIKPDQVITPAVAASYLFKKNYSKDVRIYVFGSQA
                     LKDYLKREGFTLSDEKADIVLVSLDQEMTYQKVATAMHLINEGAIFYATNRDHILASE
                     NGWLPGGGVMVSAVETCTQKKPIVIGKPQHYM"
     gene            complement(797..1396)
                     /locus_tag="PKV59_02635"
     CDS             complement(797..1396)
                     /locus_tag="PKV59_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HQP45679.1"
                     /translation="MDKPRNAVVPMVVEGGAYGERAYDIYSLLLKERIVFLGTQIDDQ
                     IANLTIAQLLYLSREDPDRDIQFYINSPGGSVYAGLAIYDTMQMIPNRINTVACGVTA
                     SFGTVLLTAGSKGHRFALPNSTIHMHQPLGGVEGQASDIEIQAKEILRLKKELNEIMA
                     KHTGQTYETILHDTDRDRYFTPEEAVAYGLIDKVLEPTK"
     gene            complement(1574..3496)
                     /gene="ftsH"
                     /locus_tag="PKV59_02640"
     CDS             complement(1574..3496)
                     /gene="ftsH"
                     /locus_tag="PKV59_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQP45680.1"
                     /translation="MSQSNNTQKNRNDKNRSGQNQLNYVLSYLIFAFLCMWLFQQFII
                     KPITVRETEITYSEFKSKIKSKEVSDVILGDYRIVGLMKNPNATSAEDEKIPFTTVAM
                     PNGDPTLIELLDENGINYTISEPANPLGSILLGYLIPLLFFGVIWYLISRQVEKSGGS
                     FGGLGGIMSIGQSKATEVKPEDVNVTFKDVGGADEAIEELQDIIQFLKEPEQFSKLGG
                     RIPKGVLLVGPPGTGKTLLAKATAGEAHVPFFETSGSEFVEMFVGVGAARVRNLFDQA
                     RKVAPAIIFIDEIDAIGQSRVGPGRFGSNDEREQTLNQLLSEIDGFKSDKGKPVIIMA
                     ATNRPEILDPALLRAGRFDRQVTVSIPDLIGRKQILQIHSKNIKLDPSFDLDRAARMT
                     PGFSGADLANVMNEAALLAARRNGNSVNMDDFELAIERIVAGLEKKTRVMNEKERTAV
                     AYHESGHALIGEIVPNGDPVAKVSIVPRGRGALGYTMQMPTEDRYLMTQSELEDKIAV
                     LLGGRAAESIIFNEISTGAADDIKKATELARRMVTEFGMSEKLGVVRYATPQYQFLES
                     YETAAVASPETLKLIDEEVQRILNEQFKRVINLLNENRDALESLTKLLLEKETISGEE
                     VKEAIAKQRIKQPAIV"
     gene            complement(3595..5097)
                     /gene="tig"
                     /locus_tag="PKV59_02645"
     CDS             complement(3595..5097)
                     /gene="tig"
                     /locus_tag="PKV59_02645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQP45681.1"
                     /translation="MKIEKQPLENHEIKLEVEISPEEFEPLKHQAAKKIAKDAKIPGF
                     RPGKAPYEIIRRNYGDTAIAQEALDTFLENQYERILKEGEIEPGGMGRLDKIDQIDPP
                     KFSLIVPLAPTVELNDYREIREEYVEAAITDEEVREILDELKTNYATAEPAEGPAKEG
                     QTVYVLIKADLKEEDPETGEKELIKERPYEFIIGKDSDEKTAWPFVNFSQNFIGAQPN
                     EIVTVEHTYGEEAPIPTLKNKTVIYTTTVQSIKDLVMPAEDLELAKKFGDYETFDDFM
                     KDIRERLLKQKQQSQENEYIENIVRQLVEKADIQYAPNALEEEAQEIFSDFKQRLAQQ
                     GVDLETYLKMKNTDMEKFLNEEIRPSAEEQLKRRLVLQEFAAKEKIRINMDNFKENVG
                     KLQETAAIDYQKAKTNKEKQAIANYITNAAMNQSFNDALFDRLIAIAKGENPAIEGEE
                     TTPESEKETAVDNLETVQNETESSNDLPVEIGSNEKIEMPESETDSENKS"
     gene            complement(5307..>5681)
                     /locus_tag="PKV59_02650"
     CDS             complement(5307..>5681)
                     /locus_tag="PKV59_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45682.1"
                     /translation="ENSCECGWPFRSVESIAGREEEFLFFTDPAGNRQFIHPIVFVEF
                     FAPGLEKFQIIQTEPNMLVLNVIIKGNKEEAIQAIEKRMDTILHQKELYDFVHYKINV
                     VNDIPNDPQTGKFRLIIPYLNS"
BASE COUNT         1456 a         1281 c         1049 g         1895 t
ORIGIN      
        1 catatagtgt tgcggtttcc cgatcacaat aggcttcttt tgggtacatg tttcaaccgc
       61 gctgaccatt acgcctccgc ccggaagcca gccgttttcg gatgccaaaa tatgatcccg
      121 attcgtcgca taaaatattg caccttcatt gatcagatgc atggctgtcg caactttttg
      181 gtaagtcatc tcctgatcca gactgactaa gacaatatca gctttttcat ccgataaggt
      241 aaaaccttcc cttttgaggt aatctttcaa tgcctgtgaa ccgaagacat aaattcgaac
      301 gtctttcgaa taattttttt tgaataaata agacgcggca acagccggtg taatcacttg
      361 gtccggtttg atttttactc caaacccggc cattttttct tgataagatt caggcgttcg
      421 tgtggaattg ttggttataa aaacaggaaa aatatccgct tgatccaatt tggtaaaaat
      481 atcccgagta tttccgatcg gatttttccc attccataat acaccatcca tatccaaaaa
      541 aattgatttt attttggaaa aatcaaaaac ttccatcaaa cccccctaag ttgatttaaa
      601 ataattataa taccgaataa attcttatct ttccccttat aagaaacggc tgcatcaatt
      661 tctttaccgc ttattgatca gggctatagt atagtcgatg atcagtaaaa tgctgttgtc
      721 tcttaagaaa tgatttcata aagattgaaa aaacctccga tatatgtcgg aggttttttt
      781 atgaagatca gaaatattac tttgtcggtt cgagaacttt atcgatcaac ccgtatgcga
      841 ccgcttcttc cggtgtaaaa tatcggtctc gatcggtatc gtgaaggatt gtttcatacg
      901 tctgaccggt atgttttgcc ataatctcat tcaattcttt tttcagtctg agaatttctt
      961 tggcttggat ttcgatatcg gaagcctgac cttcgacacc acctaatggc tgatgcatat
     1021 ggatcgttga attcggcagc gcaaaacgat gacctttcga accggctgtt aagagtactg
     1081 ttccaaaaga tgcagtcact ccacaagcta cggtattgat ccgattcggg atcatctgca
     1141 tcgtatcata aattgcaagg ccggcataga ccgatccacc gggtgaattg atatagaatt
     1201 gaatatcacg atcggggtct tccctgctta aataaaggag ctgtgctatg gtcagatttg
     1261 caatctgatc gtcgatctgt gttccgagaa aaacaattct ctctttcaac aacagtgaat
     1321 aaatgtcgta agcacgttca ccataagctc caccttcaac aaccattggc actacagcgt
     1381 ttcttggttt atccatattt attttttctc ctgtccaatc ggaatcaatt tctttcatcc
     1441 gacgaatcgt taatttgatg gatatataat aacatgagac accgataaga tttgatagaa
     1501 aattgttaga aatatttaaa agtgctgcac ccttttgttt ccgagtgcag cactcaatcc
     1561 atatcgattc gaattaaacg attgctggtt gctttattct ctgtttagcg attgcttctt
     1621 tgacctcttc acccgatatg gtctcttttt cgagcaataa ctttgtcaaa ctttccaacg
     1681 catctcggtt ttcattgaga agattaatca ctctcttgaa ttgctcattc aaaatacgct
     1741 gaacttcttc atcgatcagt tttagagttt ccggacttgc tacggctgcc gtttcataac
     1801 tttcaaggaa ctgatattgc ggggtggcat atcttaccac gcccaatttt tcactcatac
     1861 cgaattccgt aaccattcgt cgcgcaagtt ccgtagcttt cttaatatca tcagcggcac
     1921 cggtgctgat ttcattgaaa ataatcgact cggctgctct tccgcccaac aagaccgcta
     1981 ttttatcctc caattcactt tgagtcataa gatatcgatc ttccgtcggc atttgcatgg
     2041 tataaccaag tgcccctctt cctcggggaa caatgcttac ttttgcaacc ggatcgccgt
     2101 tggggacaat ttcaccgatc agagcatgcc cgctttcatg atatgcgacg gcagttcttt
     2161 ccttctcatt catcacgcgg gtctttttct caagtccggc aacaattctt tcgattgcca
     2221 actcgaaatc atccatatta acggaattcc catttcggcg agcagccagc aatgcagctt
     2281 cattcattac atttgccaaa tctgccccgc taaaaccggg tgtcattcga gctgctcgat
     2341 ccaagtcgaa actggggtcc aatttaatat ttttgctgtg aatctgcaaa atttgtttcc
     2401 gtccgattag atccggtata gaaaccgtaa cctgccgatc aaaacgaccg gcacgtaaga
     2461 gagccggatc caaaatctcc ggacggtttg tcgctgccat aataatcacc ggtttccctt
     2521 tatcggattt aaacccgtcg atttcggata acaattggtt caacgtctgt tcccgttcat
     2581 cattagatcc aaatcttcct gggccgactc gagattggcc gatcgcatcg atttcatcga
     2641 taaagataat tgcgggagcg acttttcgtg cctgatcgaa gagatttcga acccttgcgg
     2701 caccgactcc gacgaacatt tcgacaaatt ccgatccgct tgtttcaaag aacggaacat
     2761 gtgcttcccc ggcggttgct tttgccaaaa gcgtttttcc tgtccccgga gggccgacta
     2821 agagcacacc tttcggaatt cttccgccta attttgaaaa ttgttcaggc tctttcaaaa
     2881 attgaataat atcctgcagc tcttcgatcg cttcatccgc acctccgaca tctttaaatg
     2941 tcacattcac atcttccggt ttgacttcgg tggctttgct ttgaccgata ctcataattc
     3001 cgcccaatcc accgaaactg cctcccgatt tctctacttg gcgtgaaatt aaataccaga
     3061 taacgccaaa gaataggaga ggaattaaat aaccgagaag gatacttccg agcgggtttg
     3121 cgggttcact gatcgtgtaa ttaataccat tctcatccaa caattcaatt aatgtcggat
     3181 ctccgttggg catggcaact gttgtgaatg gaattttttc gtcctcagcg gaagttgcgt
     3241 tgggattctt catcaagcca acaattctgt aatctcccag tattacatca gaaacttcct
     3301 tgcttttaat ttttgattta aattcgctgt aggttatttc cgtttcacga accgttatcg
     3361 gcttgatgat gaattgctga aataaccaca tacaaagaaa agcaaaaatc aaataactga
     3421 ggacatagtt cagttgattt tgcccggatc gatttttatc atttcgattc ttttgggtat
     3481 tatttgattg actcatttta ttctccgtat agttaattca tttaataaaa atcctgaatt
     3541 aactatacgg cttttttata tgaatcttgt taattagaaa acaaaatcgc tcttttaaga
     3601 tttattttcg gagtctgttt cagattccgg catctctatc ttctcgtttg agccgatttc
     3661 taccggaaga tcattcgaag attcggtctc attttgaacg gtttcgagat tatcgacagc
     3721 cgtttccttt tcactttcgg gagttgtttc ctctccttcg atcgccggat tttcgccctt
     3781 tgcgattgca atgagacgat caaacaatgc atcattgaaa gattggttca tggcagcatt
     3841 tgtaatatag ttcgcaatcg cctgtttttc cttgttggtt tttgcctttt gataatctat
     3901 tgcggcggtt tcttggagtt taccgacatt ttccttgaaa ttatccatgt tgattctgat
     3961 tttttctttt gccgcaaatt cctgaagtac caaacgtctc tttaattgct cttccgcgct
     4021 cggtctgatt tcttcattaa ggaatttttc catatcggta tttttcatct tcagataggt
     4081 ttcaaggtcg actccctgtt gtgccaatcg ttgcttaaaa tcactgaata tttcttgtgc
     4141 ttcttcttca agtgcattcg gtgcgtattg gatatcagct ttttctacca attgtctgac
     4201 aatattttcg atatattcat tttcttgact ttgttgtttt tgttttaata atctttcacg
     4261 aatatctttc atgaaatcat caaaggtctc gtaatcgccg aattttttcg ccaactcaag
     4321 atcttcagca ggcatcacca agtctttaat gctttgtacc gttgtcgtat aaataaccgt
     4381 cttgttcttc aaagtcggaa tcggagcttc ttcgccgtaa gtatgctcaa ccgttacgat
     4441 ctcgtttggt tgcgcaccga taaaattttg agaaaaatta acaaatggcc aagctgtctt
     4501 ttcatcggaa tctttgccga taatgaattc ataaggtcgt tctttgatta actctttctc
     4561 gccggtttcc ggatcttctt ctttcaaatc agccttgatt aacacataga ccgtctggcc
     4621 ttctttggca ggtccttccg ccggttccgc cgtggcataa ttcgttttta actcatccaa
     4681 aatttcccga acttcttcat cggttatagc ggcttccaca tattcttcgc gaatctcacg
     4741 atagtcgttc agttctactg tcggtgcaag agggacgatc aaactgaatt tcggaggatc
     4801 aatttgatct attttatcta atctgcccat tccccccggt tcaatttctc cttctttcaa
     4861 aatacgttca tattgatttt cgagaaatgt atcgagggct tcctgagcta tcgcggtatc
     4921 accgtagttg cgtcgaatta tttcataagg cgctttcccg gggcggaagc ccggaatttt
     4981 tgcgtctttg gcaatttttt ttgctgcctg atgctttaat ggttcgaatt cttccggact
     5041 gatttcgact tccaatttga tttcatgatt ttcaaggggt tgtttttcga ttttcaagat
     5101 ggcttcctcg ttatatatga attaattatt tatcccgacg gaaaaatcgg ctcccaaacg
     5161 atccgaattc ttctctgtct gaacaaaccg accgaattat cggactgaat tttcagaatg
     5221 tcaagcagat cgatttttca atccgatttt ccgcctacga tattatacat taggtttatt
     5281 cgatcgatat acaagatgat acaaaatcaa gaattaagat atggaataat cagcctgaat
     5341 ttccctgttt gggggtcatt ggggatatcg ttcactacgt ttattttata atggacgaaa
     5401 tcatataatt ctttttggtg aaggattgtg tccatccgct tctcgatagc ttgaattgct
     5461 tcttccttat ttcctttaat aattacgttc agaaccagca tattgggttc agtctgaatg
     5521 atttgaaatt tttcaagccc cggcgcaaag aattcaacga agactatcgg atggataaac
     5581 tgccggttac cggccggatc tgtaaaaaac aaaaattctt cttcacggcc ggcaatcgat
     5641 tcaaccgatc ggaacggcca tccgcattcg cagctgtttt c
//
LOCUS       DAOMIV010000069         5677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14380_length_5677_cov_5.950374, whole genome shotgun sequence.
ACCESSION   DAOMIV010000069 DAOMIV010000000
VERSION     DAOMIV010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14380_length_5677_cov_5.950374"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="PKV59_02655"
     CDS             <1..1089
                     /locus_tag="PKV59_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQP45683.1"
                     /translation="KIRFDFKSNSTIPDQFPFTIWAKLMREVLSERNPELLESGPSNG
                     NEKLRISIATYLHDFRGMTVSADRIVIGAGAEYLYSLVVQLLGRDKVYAVENPGYQKI
                     AKIYRNNGAVCLPIRTDDHGISAETLQSSDAEVVHITPAHHFPLGIVMPISRRNELLA
                     WANEEEDRIIIEDDYDSEFRFQGKPVQPMINIDQNQKVIYVNTFSKTLFPSLRISYMV
                     LPDRFMQIYNQKLGFYSSTVPSFDQLVLANFIAGGYYEQHINRMRNYYKRLRNQLIFS
                     LRQSSLQDRIQIIEEDSGLHFLLKVDTTMKDEEIVKKAEILGINLSCLSEYYFDKNSR
                     ENGTLLINYSGISIEQVDEAIETLSAIF"
     gene            complement(1153..1635)
                     /locus_tag="PKV59_02660"
     CDS             complement(1153..1635)
                     /locus_tag="PKV59_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQP45684.1"
                     /translation="MKSNFSIRTASSADVKLILQFIRELADYEKMADQVTATEELLSE
                     WLFEKRIATVLIGESEGMPVGFALYFYNFSTFLGKAGIYLEDLYIRDEYRHRGFGTEF
                     MKALARIATENNCGRLEWWCLDWNQPGIDFYLSLGARPMEDWTVYRITGQSLVELANS
                     "
     gene            complement(1806..2201)
                     /gene="rpsI"
                     /locus_tag="PKV59_02665"
     CDS             complement(1806..2201)
                     /gene="rpsI"
                     /locus_tag="PKV59_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HQP45685.1"
                     /translation="MVGRYYEGIGRRKESVARVRISSGSGDFVVNDKQIADYFNRLGD
                     VESILAPFTAINQDQSGFDVSVHVNGGGVTGQTEAVRLGLARALVKMDDTLKPSLSHA
                     GLLTRDPRIKERKKPGLKRARKAPTYTKR"
     gene            complement(2213..2644)
                     /gene="rplM"
                     /locus_tag="PKV59_02670"
     CDS             complement(2213..2644)
                     /gene="rplM"
                     /locus_tag="PKV59_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HQP45686.1"
                     /translation="MTVEKTYVIKGEPESEWLLVDANGQSIGRLATQIASYLLGKHKP
                     TYSPGVKMGDFVVVINADHLAIPQKKLATKHYYRHSGYPGGLTDTTLKDMMRTYPERV
                     IESAVWGMLPHNKYGRALMKRLKIYAGNEHPHAAQNPTRVN"
     gene            complement(2676..3461)
                     /gene="truA"
                     /locus_tag="PKV59_02675"
     CDS             complement(2676..3461)
                     /gene="truA"
                     /locus_tag="PKV59_02675"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQP45687.1"
                     /translation="MSELSERGNPVPDTCERYKFTVSYDGTLYWGFQRQKGRRTIQSE
                     LETALTALGWKETGIISAGRTDSGVHADGQVFSARLAWRHSPEALRAALNARLPQDIS
                     IKDVKRVPDNFHARYDAFSRTYRYRIYFAEVRDPLRDRFRLRLWPESDLKTLNQAAHI
                     LLGEHDFRAFGSPPRKNGTTVRRVIASGWIPEDRDEIAFEIEANGFLYHMVRRMVFLQ
                     LQWATDRIDWKTWEDAVLNAKPTHPGIAPAKGLSLYRVRYEKL"
     gene            complement(3454..3834)
                     /gene="rplQ"
                     /locus_tag="PKV59_02680"
     CDS             complement(3454..3834)
                     /gene="rplQ"
                     /locus_tag="PKV59_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQP45688.1"
                     /translation="MRHGVAGYKLGRTSSQRKLLRMTQISQLFEHERIITTQAKALSM
                     RNDAEKLITLAKKGNQGDKIQKMNAARLASSRLGNDRELTKKLFDDIAPRYDKRNGGY
                     TRMIKLGPRKGDAAEMVVIELVGE"
     gene            complement(3840..4805)
                     /locus_tag="PKV59_02685"
     CDS             complement(3840..4805)
                     /locus_tag="PKV59_02685"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQP45689.1"
                     /translation="MATLSNMVTPKVEREREAENYGKFVVYPLERGYGVTLGNALRRV
                     LLSSLEGVAVTSLHISDVMHEFSTIPGVREDVLQVMLQIKQIRLKMYEGDVSHLRLEV
                     NGEGEVTASDIQCPAEVEILNPELYLFTVDNPDTHLEIDMTVERGRGYLPANDRTNRL
                     PIGELPVDAIFSPIKRVNWNVGSALYGQSTNYDKLELEIWTDGTIQPQAALILASKIL
                     IEHLRYISGITEEVLPDTIVTEQHSTHVTSETAETPIESLDLSVRVFNSLKRTGVATV
                     GDVLDLLDKGDDAVKSIRNFGEKSLEELREKLQEKGYLKKESDTE"
     gene            complement(4862..5503)
                     /gene="rpsD"
                     /locus_tag="PKV59_02690"
     CDS             complement(4862..5503)
                     /gene="rpsD"
                     /locus_tag="PKV59_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQP45690.1"
                     /translation="MARYTGSVCKLCRRENEKLFLKGERCFSAKCSFEKRAYAPGDHG
                     RSGGSRGGERVSDYSRQLRAKQKVRRKYGVLERQFRRVFGIAVRQSGLTGLNLLQLLE
                     TRLDNVVYRLGYAENRAQARQMVTHGHFEVNGIPSDIPSMILKPGDQISVRKSSASKA
                     LFSDLADIADKRVCPLWLDRNTKALSGRVLRLPERSEIDGNINEQLIVEFYSR"
     gene            complement(5525..>5677)
                     /gene="rpsK"
                     /locus_tag="PKV59_02695"
     CDS             complement(5525..>5677)
                     /gene="rpsK"
                     /locus_tag="PKV59_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQP45691.1"
                     /translation="QEVDLIIKGPGPGRESAIRAVQGMGMKVRSISDITPVPHNGCRP
                     PKKRRV"
BASE COUNT         1516 a         1435 c         1142 g         1584 t
ORIGIN      
        1 aagatccgat tcgattttaa atccaacagc accattccgg atcaattccc ttttacaatc
       61 tgggcaaaat taatgcgtga agttttatcc gaaagaaatc cggaactatt ggaatccgga
      121 ccatccaatg ggaatgaaaa gctccgaatt tccatagcaa cttatttgca tgattttcgc
      181 ggaatgacgg tttctgcgga tcgaattgtg atcggtgccg gagcggagta tctgtacagt
      241 ttggtcgtgc agcttttggg cagagataaa gtctatgcgg tggaaaaccc gggctatcag
      301 aaaatcgcca aaatatatcg caataacggt gccgtttgtc tgccgattcg aacagatgat
      361 catggaatct ccgcggagac cttacagtct tcggatgccg aggttgtgca cattactccg
      421 gctcatcact ttccgctggg catcgtcatg ccgatttcga gacggaacga actcttggca
      481 tgggcaaacg aagaagaaga ccgaatcatc atcgaagatg attatgacag cgagtttcgc
      541 tttcaaggaa aaccggtcca acccatgatc aatatcgatc aaaatcaaaa agtaatttac
      601 gtcaatacct tttccaagac tctgtttcct tcgctgcgaa tcagttatat ggtattgccc
      661 gatcgattca tgcaaatata caaccaaaag ttgggatttt actcatcaac cgtaccaagt
      721 ttcgatcaac tggttctggc aaattttatt gccggaggct attatgaaca gcatatcaat
      781 cgcatgcgga actattataa aaggttacga aaccaattaa ttttttctct gcggcaaagt
      841 tctttacaag atcggattca aatcatcgaa gaagattccg gtttgcattt tttactgaaa
      901 gtagatacaa cgatgaaaga cgaggaaatc gtcaaaaaag ctgaaattct tgggatcaat
      961 ttgtcttgtc tgtccgaata ttattttgat aaaaacagcc gtgaaaacgg gacattattg
     1021 atcaactact ccggaatatc gatagaacaa gtcgatgaag cgatcgaaac tttaagcgca
     1081 atattctaaa accccaaaac cgcccttgac gtacaaaaac aggttttata atctcatctc
     1141 tcccattccg catcaagaat tcgccaattc caccaaggat tggcctgtaa ttcgataaac
     1201 cgtccaatct tccataggcc gggctcccaa agaaagataa aaatcgattc ccggctgatt
     1261 ccaatccaaa caccaccatt ccaaccttcc gcaattattt tcggttgcga tccgcgccaa
     1321 tgctttcatg aattcagtgc cgaaacctcg gtgacgatat tcatcgcgaa tatacaaatc
     1381 ttccaaataa atacccgcct tcccgagaaa tgtcgaaaaa ttataaaaat acagtgcaaa
     1441 accgaccggc attccctctg attcgccgat taagacagtg gcaatccttt tttcaaagag
     1501 ccattcggat agcagttctt cggttgctgt cacttgatct gccatttttt catagtcagc
     1561 cagttcacgg ataaattgca aaattaactt aacatcggca gatgaagcgg ttcgaattga
     1621 gaaattgctc ttcatcgttt tttatgcctc caaaataatt gatttttcac cgaaaaatat
     1681 tttgattttt atcgacgatc cgatcttgtt tttccgatca agtggatatc gaactttcct
     1741 gcgatattaa aaaacccgtc caaactcgaa cgggttttcc ataatacaaa ctctgacaca
     1801 gataattaac gctttgtgta agtcggtgcc ttgcgagcgc gtttcaaacc cggcttcttt
     1861 cgttctttaa tccgtgggtc gcgagtcagt aatcccgcat ggctgagtga cggtttcagc
     1921 gtatcatcca tcttaaccaa cgcccgtgcc aatcccaacc gaaccgcttc ggtttgaccg
     1981 gtcacaccgc ctccgttcac atgaacagat acatcaaacc ctgattgatc ttggttaatt
     2041 gcggtaaaag gcgcgagaat cgattcgaca tcgccgaggc gattgaaata atccgcgatt
     2101 tgcttatcat ttaccacaaa atcaccgctt cccgaactga tgcgtacccg agcaacactc
     2161 tccttgcgac gtcctattcc ttcataatat cgtccgacca ttcttttact ccttaattca
     2221 cacgtgtcgg attctgagca gcgtgtggat gctcattacc agcataaatt tttagccgtt
     2281 tcatcaacgc acgtccatat ttattgtgcg gaagcatgcc ccaaacagcc gattcgataa
     2341 cacgttccgg atatgttcgc atcatatctt ttaatgttgt atccgttaag cctccgggat
     2401 aaccggaatg gcgataataa tgtttcgtcg ccaatttctt ttggggaatt gccagatgat
     2461 cagcattgat cacaaccaca aaatcgccca tctttacacc cggggaatat gtcggtttat
     2521 gtttccccaa taaataactt gcaatttggg ttgccaaacg cccaatgctc tgcccgttcg
     2581 catcaacaag cagccattcg ctttcaggtt cacccttaat gacataggtc ttttccacag
     2641 tcatctctcc aattcctaat aaactttttt ctgattcaca gtttctcata tcggacccgg
     2701 taaagactta accccttcgc gggagcaatt cccggatgag tcggtttcgc attcagcacc
     2761 gcatcttccc aggtcttcca atcgattcga tcggtagccc attgaagctg caaaaatacc
     2821 atccgcctta ccatgtgata gagaaatcca ttcgcttcta tttcaaatgc gatctcatct
     2881 ctatcttcgg gaatccaacc ggacgctatt actcgccgaa ctgtggtgcc gtttttccgg
     2941 ggcggggaac caaaagcacg aaaatcatgt tcgccgagca aaatatgagc ggcttgattc
     3001 agggttttca aatccgattc gggccataac cgcagtctga aacgatcacg aagcggatcc
     3061 cggacttcgg caaaataaat tcgatatcga taggtccggg aaaacgcatc atagcgtgca
     3121 tggaagttat ccggtactcg cttgacatcc tttatggaaa tatcttgcgg cagtctcgcg
     3181 ttcaaggctg cccgcaaagc ttccggactg tgacgccaag caagtctcgc cgaaaaaacc
     3241 tgtccgtcgg catgaacgcc gctgtcagtc cttccggcgc tgataattcc tgtttccttc
     3301 caacccagcg ctgtcaaagc tgtttcgagc tctgactgga tcgttcttcg ccctttttga
     3361 cgttgaaagc cccaataaag cgttccgtca taagaaactg taaatttgta acgttcacaa
     3421 gtgtccggaa cgggatttcc ccgctccgac aactcactca ccgacaagtt cgatgacaac
     3481 catttccgca gcgtcccctt ttcgagggcc cagcttaatc atgcgggtat acccgccgtt
     3541 tcgtttatca taccgtgggg caatatcgtc aaacaacttt ttggttaatt cacgatcatt
     3601 gcctaaccga gaagaggcta agcgggcggc attcattttt tggattttat cgccctgatt
     3661 gccttttttt gccaatgtaa tcagtttttc agcatcgttg cgcatcgata aagctttggc
     3721 ttgtgttgta ataattcttt catgctcaaa cagctgagaa atttgcgtca tacgcagtaa
     3781 cttccgctga gatgacgttc ttcctaattt ataacctgca actccatgcc gcatttcaat
     3841 tactccgtat cactttcttt tttgagatag cccttttcct gcaatttctc tcgtaattct
     3901 tcaagactct tctcgccgaa attacggatt gatttcacag catcatcccc tttgtccaat
     3961 aaatcaagca catctccgac ggttgcaaca ccggtccgct ttaatgaatt gaagactctg
     4021 accgacaggt caaggctctc aatcggtgtt tcagcggttt cactcgtaac atgtgtactg
     4081 tgttgttccg ttacgattgt gtccggcagt acctcttcgg ttatgcccga aatataacgg
     4141 agatgctcga ttagtatttt ggaagctaaa atcagagcgg cctgtggctg aatggttcca
     4201 tccgtccaga tttccaactc taacttatcg tagttcgtac tctgtccgta aagagcagag
     4261 ccgacattcc aattgacgcg tttaatcggg ctgaagatcg catcaaccgg cagctctccg
     4321 atggggagac ggtttgtcct gtcattcgcc ggcaaataac cgcgtccgcg ttcaacggtc
     4381 atatcgattt ccaaatgggt gtcggggttg tccacagtga aaagatataa ttcaggattt
     4441 aggatttcca cttcagcagg gcattgaata tccgaagcag ttacttcccc ctctccattt
     4501 acttcgagac ggagatgtga gacatcgcct tcatacattt tgaggcgaat ctgcttgatt
     4561 tgaagcatta cctgtaaaac gtcctcgcga actccgggaa ttgtgctgaa ttcatgcatc
     4621 acatcagaga tatggaggga cgtaacagcc accccttcca atgatgagag caaaacccgt
     4681 cgcaatgcat tccccaaagt cacaccgtag ccacgttcga gagggtaaac tacgaattta
     4741 ccgtagttct cagcttcacg ttcccgctca actttcggtg ttaccatatt gcttaatgta
     4801 gccaattttc acccctttcc gatacaacaa aaaccaaatc tgtatcggcg cctaattcat
     4861 attaccgtga gtaaaactca acgatcaatt gttcgttaat attcccgtcg atttcacttc
     4921 gttccggcag acgcagcaca cgaccgctga gagctttcgt gttccggtcc aaccataacg
     4981 gacaaacacg tttgtcggct atatcagcca aatcgctgaa aagcgccttt gaagcggaac
     5041 tttttcgaac cgaaatctga tcgccgggtt ttaagatcat cgaagggata tcgcttggga
     5101 tgccattaac ttcaaaatga ccgtgtgtga ccatttgacg agcttgagca cggttttccg
     5161 cataccctaa ccggtatacg acattatcta aacgagtttc caaaagctga agtaaattta
     5221 acccggttaa accggattgt cttactgcaa tgccgaatac ccggcggaac tggcgttcca
     5281 aaacgccata ttttcttcgt actttttgct tcgcacgcag ctggcgggaa taatccgaaa
     5341 ctctttcccc tccgcggctg ccgccggatc taccgtgatc accgggggca taagcgcgtt
     5401 tttcaaaaga acattttgca gaaaagcaac gttcgccttt aaggaataat ttttcatttt
     5461 cgcgtcggca taatttacat accgacccag tatatcttgc cattgttctt tgttcctttc
     5521 gtaattaaac gcggcgtttt ttcggaggac gacaaccgtt atgcggtacc ggagtaatat
     5581 ccgaaatcga acgaactttc atccccatcc cttgaacggc acgaatcgca gattcgcgac
     5641 ccggacccgg gcctttaatg atcaagtcca cttcctg
//
LOCUS       DAOMIV010000070         5657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14458_length_5657_cov_4.467333, whole genome shotgun sequence.
ACCESSION   DAOMIV010000070 DAOMIV010000000
VERSION     DAOMIV010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14458_length_5657_cov_4.467333"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /gene="rsgA"
                     /locus_tag="PKV59_02700"
     CDS             <1..774
                     /gene="rsgA"
                     /locus_tag="PKV59_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HQP45692.1"
                     /translation="DLVEFTQLQDGTGIIECILPRKNELIRMISGIKSEYRQVLISNL
                     DQILLVFAVTQPEPRLRMLDRFLVICEKQGIPPLIVANKVDLSGMDQAKSVFSVYSDI
                     GYDVLYTSTASDIGIADLKERLQGKVTGLVGPSGVGKSSLINRIDPRLNLKVNEISNY
                     NRKGKHTTVVREMFPLKEGGFVADLPGLKTLALWDIQPEELDGYFPEISPLVSKCLYG
                     DCTHSPSEVGCAVHEAVKEGKISRERYESYLRIRFGEPE"
     gene            878..1915
                     /locus_tag="PKV59_02705"
     CDS             878..1915
                     /locus_tag="PKV59_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HQP45693.1"
                     /translation="MPKRLTWTELILNLKSPFTVSYGSSDTRTAYWIRLEEDSGWGEG
                     TIPFYYGIDFKDMTDFWDRCALREDPFPETINAIHPWIGDDAPAPAIAALDIAFHDRI
                     GKKTGKAIYELYDLPAPHPMTTSYTISIDTPENMAQMALRINRYPLIKIKLGSPGQED
                     LDEARIAAIRTVRPDAKLRLDANAGWKTDDAVKLVKRLEKYNIELIEQPTAKFDILGM
                     GHVQTETPIPIVADESLQTINDIEKLHDVGVRGINIKLMKCGGIAPAVEMIRKARSYG
                     MKIMLGCMVETSIGVGAMASLIGLADWIDLDTPLLISNDPFSGLTYTEDATLIPPAGP
                     GLGLKLKNSER"
     gene            1924..2940
                     /locus_tag="PKV59_02710"
     CDS             1924..2940
                     /locus_tag="PKV59_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQP45694.1"
                     /translation="MTFQKIETILADVKKNFFNEPMLTVFDIQPEIISSNELKLTGKI
                     LEQKDLDFLKNELENKIPDWKVDYKGIKVLLNEKTTILRVSKNLTDMHREKSFLSELT
                     NQMLYGDPVAVLADEGEWVLGRNMSDGYISYTYKKYLGEIPWSEPTHIVSEPVALAYN
                     NPNREELVTRVLGGTETVIFDEKGDMAFIRADHNGWVKKTSLIPISDLPTDQDKIRNR
                     VCENAKSLIGVPYLWGGTSACGIDCSGLVQWSYRLCNIRLRRDAHMQLIPEKCVEPPF
                     LPGDVVLYGEEENGKKSITHASISLGGWTVIHSSRSQNGVYIDDALIAPALKGTFAAA
                     VRYI"
     gene            complement(3009..3761)
                     /locus_tag="PKV59_02715"
     CDS             complement(3009..3761)
                     /locus_tag="PKV59_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HQP45695.1"
                     /translation="MDRERIEKELSTLDGEYTFYYSDQENHPIAVSNRDESHRFLTAS
                     VIKVPLLFAWYVSEKEGFVSSDEICDPASEPIVKGAGFYYLFHQKALPYHDLLLMMIA
                     TSDNYCTNLIINKLGFERINHIFSETFHFRDTHLGRKMMSKPDLARGYDNWTTTADMI
                     YCYDLFHSFPAEAKKNMSEMLSVCQADKLFTRNLKGDSVDFYHKSGGLHNVINEWGFT
                     DRCRIFLLCNNYSDYQKVYDVFGNLGKWLLDS"
     gene            3832..4176
                     /locus_tag="PKV59_02720"
     CDS             3832..4176
                     /locus_tag="PKV59_02720"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HQP45696.1"
                     /translation="MVYHICTQTSYESVKIRGKYSPDSLGKEGFIHFSSIRQILETAN
                     SFYRGQDDLVLLEVDEQLTDQIRFERPADHREGLFPHYYGELPVELIRRVLPFPHQAD
                     GTFKLPEEIAES"
     gene            4177..5496
                     /locus_tag="PKV59_02725"
     CDS             4177..5496
                     /locus_tag="PKV59_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HQP45697.1"
                     /translation="MLDESQFKLIDELIRAALTAVDPENVVNNALDLKNSILKIQNQP
                     FDLSEFEHIYLIAFGKASLKMAKATDDLIGRFITGGIIVSKKKNSEIRLPDKYTCLVG
                     GHPIPDQNSLESAQRILNLVKDAGNRDLIICLISGGGSALLTLPENGISLKDIADLTK
                     LLLENGANIQEINAIRKHIDRIKGGKLAIAAAPTRIVTLILSDVIGNSLEAVASGPTV
                     PDSTTYRDCLEIIQKYHLSEKIPAAIYERILIGAGGDIPETLKDDSPILKNISNYLIG
                     DNSTAVSAIVEKGKSLGLNVSVIPKPIIGEAREAGRFLAAELRKPVTFKPSLLVGGGE
                     TTVTIKGKGLGGRNLETALAAVKDLAGIPNIALVTLATDGEDGPTDAAGAIVTGETFS
                     EGMKKGLDPDLFLDNNDSYHYFEQMGGLIKTGSTGTNVNDLVFLFHF"
BASE COUNT         1679 a         1176 c         1257 g         1545 t
ORIGIN      
        1 gatttagtcg agtttacgca gcttcaagat gggacaggta ttatcgaatg tatcctgccg
       61 cggaaaaatg aactaattcg aatgatttcc ggtatcaaat cggaatatag acaagtttta
      121 atcagcaatc tggatcaaat tcttttggta tttgccgtta cacagcctga gccgcgtttg
      181 agaatgttgg accgtttctt ggtgatctgt gaaaaacaag gcataccgcc gttaattgtg
      241 gcaaataaag ttgatttatc aggtatggat caggcgaaat ctgtattctc cgtctattcg
      301 gatatcggct atgatgtttt atatacctcg acggcttccg atatagggat cgcggatcta
      361 aaagaacgat tacagggaaa agttaccggt ttggtcggcc cctccggcgt cggtaagtcc
      421 agtttgatta atcgaatcga tccccgcttg aacttgaaag tcaatgaaat cagtaattac
      481 aatcggaaag gaaagcatac aacggtcgtg cgggaaatgt tccccttaaa agagggtggg
      541 tttgtcgcgg atctaccggg actcaagacg cttgcacttt gggatattca accggaagaa
      601 ttggacggtt actttcccga aatcagtcca ttggtttcaa aatgtctata cggagattgc
      661 acacacagcc cttcggaagt tggttgtgcg gttcatgaag cggtaaaaga aggcaaaatt
      721 agccgggaac gttatgaatc gtatcttcgg atccgattcg gcgaaccgga atagctcccc
      781 ttttttccgg tttcatatat atttttctat catttgcagt caaacagaaa taatactgtt
      841 agaatcgagc tatcatccga ttcataaaag gattgccatg ccaaaaagac tcacttggac
      901 tgaattaata cttaatttaa aatctccttt tactgtttct tacggaagtt ccgatacacg
      961 gaccgcttat tggatccgac tcgaagaaga ttccgggtgg ggagaaggga caatcccttt
     1021 ttattacggc attgatttta aagacatgac tgatttctgg gatcgttgtg cattgcggga
     1081 agatcctttt ccggaaacaa ttaatgcaat tcatccatgg atcggcgacg atgcacccgc
     1141 acctgccatc gcggctttgg atatcgcttt tcacgaccgg atcggcaaaa aaaccggaaa
     1201 agctatttat gaactttatg atcttccggc cccccatccg atgacaacct cttatacaat
     1261 cagtattgat acaccggaaa acatggcaca aatggctctc cgaatcaatc ggtacccgtt
     1321 gatcaagatt aagctcggtt cacccggcca agaagatttg gatgaagcac ggatagcggc
     1381 aattcggacc gtacgtccgg atgcaaaatt aaggttagat gccaatgccg gttggaagac
     1441 ggacgatgcc gtcaaattag taaaacgtct tgaaaaatat aatatcgagc tgatcgaaca
     1501 gcccactgcg aaatttgata ttctcgggat ggggcatgtt caaacagaaa cgccgattcc
     1561 gattgtcgca gatgaatcct tgcaaacaat caatgacatt gagaaattgc atgatgtcgg
     1621 agttcgggga atcaatatta aactgatgaa atgcggaggt attgcccccg ctgttgagat
     1681 gattcggaag gcccgttcat acggaatgaa aatcatgctc ggctgtatgg tcgaaacttc
     1741 catcggtgtc ggtgcgatgg cttctttgat cgggttggct gattggatcg atctggacac
     1801 tccattgctg atttccaacg acccgttcag cggacttact tacaccgaag acgcaacact
     1861 gatcccgccg gccggacccg gattgggatt gaaacttaaa aactccgaaa ggtaggggaa
     1921 aatatgacat ttcaaaaaat agaaaccatc cttgctgacg ttaagaagaa tttttttaat
     1981 gaaccgatgc tgaccgtttt tgacattcaa cccgaaatca tttcatcaaa tgaattaaaa
     2041 ctgaccggta aaatactcga gcaaaaagat cttgatttcc ttaaaaatga attggagaac
     2101 aagattccgg attggaaggt cgattacaaa gggataaaag tattactgaa tgaaaaaacg
     2161 acgattttac gcgtgagcaa gaatctcacc gatatgcatc gtgaaaaatc ttttctatcg
     2221 gaactcacca atcagatgct ttacggagac cccgttgctg ttctggctga cgaaggagaa
     2281 tgggttttag ggcgcaatat gtcagacggg tatatcagtt acacttataa aaaatacctc
     2341 ggcgaaatac catggtcgga accgacccat attgtcagcg aaccggtggc attggcttac
     2401 aacaatccga atcgggaaga gttggtgacg cgagtgcttg gcggaaccga gacggttata
     2461 ttcgatgaaa aaggagatat ggcattcatt cgagcggatc acaacggatg ggtgaagaag
     2521 acttctctga ttcccatttc ggatctgccg accgatcagg ataaaattcg aaatcgggtt
     2581 tgtgaaaatg cgaaaagtct gatcggtgtt ccctatttat ggggcggtac ttctgcgtgc
     2641 gggatcgatt gttccggatt ggttcaatgg tcttatcgat tatgcaacat ccggcttcgc
     2701 agagatgcac atatgcagct tattcccgaa aaatgtgttg agcctccgtt tttacccggg
     2761 gatgttgttc tttacggaga agaagaaaac ggtaagaaat ccatcaccca tgcatccatc
     2821 agtttgggag gatggactgt cattcattca tccagatcac aaaacggcgt ttatatcgat
     2881 gatgccttaa tcgctccggc tttaaaagga acttttgcgg ctgccgtacg atatatttaa
     2941 atcatttgat tcggatcaaa ataattgtca ttttttgttg atcggaatat tatgtaactg
     3001 gttacttttt atgaatcaag cagccattta ccgagatttc cgaaaacatc atagactttc
     3061 tgatagtccg aataattatt gcaaagcaaa aaaatccgac agcgatcggt aaatccccat
     3121 tcgttgatca cattatgcaa accgccggat ttatgataaa agtctaccga atcacctttg
     3181 agattgcggg tgaaaagttt atccgcctgg caaaccgata acatttcaga catattcttc
     3241 tttgcttctg ccgggaaaga atggaacaga tcgtaacagt aaatcatatc cgcagttgtc
     3301 gtccaattgt cataaccccg tgccaaatcg ggttttgaca tcattttcct tcccagatgg
     3361 gtatcccgaa agtgaaaagt ctccgaaaag atgtgattaa tcctttcaaa acccaattta
     3421 ttaatgatca aattcgtaca gtaattatcc gaagtcgcaa tcatcatcag caataaatca
     3481 tgatatggta atgctttctg atggaataaa taataaaaac ccgcgccttt gacgatcggt
     3541 tcagaggcag ggtcacaaat ttcatccgaa gaaacaaatc cctccttttc cgaaacatac
     3601 caagcaaata ataacggcac tttgataacc gatgctgtta gaaatctgtg agattcatcc
     3661 cgattggaaa cagctatcgg atgattttct tgatccgaat agtagaaagt atattcccca
     3721 tccagtgttg aaagttcctt ttcgatccgt tctctgtcca tgataaagtc ctccgctttc
     3781 tgattataat tttgattata gaaaaccctt tcatgaaacg gagataattc attggtatat
     3841 catatttgca ctcaaacctc atatgaatca gtcaaaattc ggggaaaata ttccccggac
     3901 agtctgggga aagaaggatt tattcatttc tcttcaatcc gacaaatcct tgagacggcc
     3961 aattcctttt atcggggtca ggatgattta gttttattgg aagtcgatga gcaacttacc
     4021 gatcaaattc gatttgaaag gcctgcagat caccgtgagg gtcttttccc gcattattac
     4081 ggagaattac ccgtagaact cattcgaagg gttctgccgt tcccgcatca agcagacggg
     4141 actttcaaat tgcctgaaga aatcgcggaa tcataaatgc ttgatgagtc tcaattcaag
     4201 ttgatcgatg aattgattcg agccgcctta accgctgtgg accccgagaa tgttgtaaat
     4261 aatgctttgg atttgaagaa ttcaatcctt aagatccaaa accaaccgtt tgacttatct
     4321 gaatttgaac atatttattt gatcgctttc ggaaaagcct cactgaaaat ggcaaaagcg
     4381 acggatgatt taatcggccg attcattacc gggggaatta tcgtatcaaa aaagaagaat
     4441 tcggaaattc gattgcctga taaatatacc tgtctggtgg gaggacatcc gatcccggat
     4501 caaaacagcc ttgaaagtgc acaaaggatc ttaaatctgg tgaaagatgc cgggaatcga
     4561 gatctgataa tatgcttgat ctccggcgga gggtccgcat tactgactct gcccgagaac
     4621 ggtatatcgt taaaggatat tgcagacctg accaagctgc ttttggaaaa cggagccaat
     4681 atccaggaaa tcaacgccat ccgaaaacat atcgaccgaa taaaaggggg aaaattagca
     4741 atagcggcag caccgacacg aatcgtaact ttgattcttt ccgatgtgat cgggaattcc
     4801 ttggaagccg tcgcatccgg gccgacagtc ccggattcaa ccacctatcg agattgtctt
     4861 gaaatcattc aaaaatatca tctctcggaa aaaataccgg ctgcgatcta tgaacgaatt
     4921 ctgatcggag ccggcggtga cattcccgaa accctgaaag atgattctcc gatcctgaaa
     4981 aatatctcga attacttgat cggcgataat tccaccgccg tcagtgccat cgttgaaaaa
     5041 ggaaaatcgc tgggattgaa tgtctcggta attccgaaac ccatcatagg tgaagctcgg
     5101 gaagccggtc gttttttggc agccgaatta aggaaaccgg ttacctttaa accttctctt
     5161 ctggtcggcg gaggtgaaac aaccgttaca atcaaaggaa aagggttggg cggaagaaac
     5221 ttggagaccg ctttggcggc tgtcaaagat ttggcgggga ttccgaatat cgctctcgtt
     5281 acgcttgcaa ccgatggtga agacggaccg accgatgccg ccggtgcgat tgttaccggc
     5341 gaaacctttt cggaaggcat gaaaaaaggg ttggatcccg atctgtttct tgataataat
     5401 gattcttacc actattttga acaaatggga ggattaatca aaaccggttc gacgggtacc
     5461 aatgtcaacg atttggtttt cctttttcac ttttgaattt aatccgaaaa atcgatgtaa
     5521 ttttctttat cgcttattta aaacaatttt ttttcctatt tgatcctttt agaaagctaa
     5581 acgttccttt cgttttcggg aacggtgtga atcaaaagaa aatgttaccc aaaccccgaa
     5641 aaaatataga gatgtaa
//
LOCUS       DAOMIV010000071         5655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14466_length_5655_cov_4.947321, whole genome shotgun sequence.
ACCESSION   DAOMIV010000071 DAOMIV010000000
VERSION     DAOMIV010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14466_length_5655_cov_4.947321"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PKV59_02730"
     CDS             complement(<1..199)
                     /locus_tag="PKV59_02730"
                     /inference="COORDINATES: protein motif:HMM:NF022030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQP45698.1"
                     /translation="MKDSSLTLALSILGFMMTFIWGKPLIQLLHRFNIGKLVRIDGPD
                     THFAKIGTPTMGGVMFIIPTLL"
     gene            complement(196..1608)
                     /gene="murF"
                     /locus_tag="PKV59_02735"
     CDS             complement(196..1608)
                     /gene="murF"
                     /locus_tag="PKV59_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQP45699.1"
                     /translation="MFSLNEILEALTGQQLAEKNRFFSEAVVDSRQAISGSMFVAIAG
                     ENTDGHQFIGEAFRRGALAALVQEDTSDKFTTVDLRTEKLPESFPPFPFCLRVDNTVA
                     ALQKTAAFHRDRLNLKVIGITGTVGKSTTKELIAEVLSQKYHTLKNTGNLNNEIGLPL
                     TLLRAGKGHQVAVLEMGFYVPGEIKLLCDISKPEIGVITNVGTVHAERAGSIEVIAAG
                     KSELVQALPKDGYAILNYDDPWVRPMAEKTSAHVFYYGLDPKADLWADQIESHGLEGI
                     RFCFHYQNESFFVRIPLIGRHSVHTALRAAAVGKIMGISWEDIFMGLNRGQSQLRMAV
                     MHSEDGAMIIDDTYNASPESVMAALNLLQEMDGYKIAILGEMRELGQYEENGHILVGA
                     RAAEVCDELIAVGEKTKKMVSSALKGGMDPQRVKWFATVQETIEYTRQRGFKKGEVVL
                     IKGSRGMQMERIVEAMERII"
     gene            complement(1692..3518)
                     /locus_tag="PKV59_02740"
     CDS             complement(1692..3518)
                     /locus_tag="PKV59_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQP45700.1"
                     /translation="MNSNIDSKFKKLAICLSIFAVLVIGRILFLQTNASAVELKETIG
                     KAYEYTTLSVSSERGNIYDREGYLLAGNSISYTISLKLQASNTHGEFIANYLAPILGI
                     DREKIIQAAETPYVENEAVEVPLTNFATKEQVDKIELAKKSLNERNTTSFESKTRVFE
                     NLNAVVYYPNIYRYYPDGDLASNIIGFYPYLNPASGASFGIEQYYDDILSGETIQKKF
                     SLDPNVPESIPDLPKGASIALTIDRKVQKLVEEQVKLAVEQQKAKSGTIIIANPKNGE
                     ILAMATYPRINLNHYWESATTFTRENKFNPAVMQPYEVGSVFKVITLAIGIDSGVIKP
                     ETVFNDTGTFNVMGIDIYNWDRGAWGPQTMIGCMQHSLNTCLSWIAEQITAPVFYDYL
                     RKFHLDKPTGIELALEDYYPILQPGDEGWTEISLPTNSFGQGLMTTPIQMIQAVSAIA
                     NDGIMMKPHIVKAIYYNDHTEVIQPEVVGRPISAETAHTVTDMLETSLEVEASDALLD
                     GVKIAGKTGTGEIAKEGYGYVLNVTNASFVGWGPTDDPQFIAYVWLQEPLISIWGSEV
                     SAPLFSDVMNEIMPYLHIPYDRQRACLDSKTCPTEVPKNEYY"
     gene            complement(3515..3979)
                     /locus_tag="PKV59_02745"
     CDS             complement(3515..3979)
                     /locus_tag="PKV59_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45701.1"
                     /translation="MKTEQHAYRLAPWRKQLKSIINILLVLVLSACIASVYLNYSAQM
                     TDMKLQIQILHDQRNELTRTIADYVTREGILTSYALMEKKATQAGYIKIDFDDEDIYT
                     YLPIPGYIPDIDILSERRVQLPPLPVSPLKPEYTESLQQYIFRKLPTKESEK"
     gene            complement(3976..4935)
                     /gene="rsmH"
                     /locus_tag="PKV59_02750"
     CDS             complement(3976..4935)
                     /gene="rsmH"
                     /locus_tag="PKV59_02750"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQP45702.1"
                     /translation="MTQETLSHQPVLLNEVVEALRPEPGKWYLDGTLGAGGHSEAILN
                     ASAPSGKLIGMDLDPNAIEMAAKRLAKFGERVKIIRGSYAFADRYITSCFDNITGFDG
                     ILLDLGISSMQVDAAEKGFSFQKEGPLDMRFDPNQKTSAYDLVNFASEEELSRIFWEY
                     GEERAARRIAAAICSARVKEPIETTTMLAEIVANAVHTNRESKNNPATKSFQALRIAV
                     NNELETIEKALPILTALLKPGGRLAVISFHSLEDRIVKNFFRIESTDCLCPPEQVVCT
                     CGHKASLKRINRHAITAKDDEIRENPRARSAKLRVVEKIETEK"
     gene            complement(4932..5375)
                     /locus_tag="PKV59_02755"
     CDS             complement(4932..5375)
                     /locus_tag="PKV59_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQP45703.1"
                     /translation="MFTGIHEHTVDKKGRTCLPARFRNLLAEGAYISVGIDKNLVIYP
                     LPYFEKLANEISSFNLADPVARKYRRRMFNKAEPIEFDANGRFLIPPFLRDEFNFQDG
                     DKIIFAGSDEYIEIWTLDEWKKAEEEVFHDDSVTTEFARLMSNKI"
BASE COUNT         1421 a         1370 c         1175 g         1689 t
ORIGIN      
        1 tcaataatgt cgggataatg aacatgactc cgcccatggt tggggtgccg atctttgcaa
       61 aatgggtatc gggaccatcg attcgtacca atttcccgat attgaatcgg tgcagcaatt
      121 gaatcaacgg tttcccccaa atgaaggtca tcataaaacc gagaatagac agggcaagtg
      181 ttaatgagga atctttcata ttattctctc cattgcttca acgattcgtt ccatttgcat
      241 tcctctggat cctttaatca gaaccacttc gcctttcttg aaaccgcgtt gccgagtata
      301 ttcgattgtt tcctgaacgg tagcaaacca tttgactctt tggggatcca ttcccccctt
      361 taaagcgctg gaaaccattt tctttgtttt ttcgccgacc gcgatcagtt catcacaaac
      421 ctcggctgcc cgcgctccga ctaatatatg gccgttttct tcatattgtc ccaattcacg
      481 catctccccc aaaatggcaa ttttatatcc gtccatttcc tgcagcaggt ttaatgcagc
      541 catgaccgat tcgggagatg cgttgtaagt atcgtcaatg atcatggcac cgtcttccga
      601 atgcatgacc gccattcgaa gctgcgattg tccacgattt aatcccataa aaatatcttc
      661 ccaactgatt cccattatct tccctaccgc agccgcccgg agagcggtat gaaccgaatg
      721 tctgccgatc agaggaatgc gcacaaagaa actttcgttc tgataatgaa agcagaaacg
      781 gattccttcc aatccatgac tctcgatttg atccgcccac aggtccgcct tcgggtccag
      841 cccgtaataa aaaacatgcg cggacgtttt ttcggccatc ggtctgaccc aggggtcgtc
      901 ataattgagg atcgcataac cgtctttcgg caaagcctga accagttcgc ttttcccggc
      961 agcaatcact tcgatggatc cggcccgttc cgcatgaacg gttccgacat ttgtgatcac
     1021 accgatttcg ggttttgaaa tatcgcagag cagttttatt tccccgggaa catagaaacc
     1081 catctccaaa accgcgactt gatgaccttt tccggcgcgc agaagtgtca gcggcagacc
     1141 gatttcgtta ttgagattgc cggtattttt caaggtgtgg tatttttggc ttaaaacttc
     1201 cgcaattaat tcttttgtgg tggattttcc gacggtaccg gtaattccga ttactttcag
     1261 atttaaccga tcccgatgaa aagcggctgt tttttgcagc gctgcgactg tgttgtcaac
     1321 gcgcaggcaa aacgggaatg gcggaaaaga ttccggaagt ttttccgtcc gtaaatcaac
     1381 ggtcgtaaat ttatccgaag tatcttcctg cacaagtgcc gccaaagccc ctcttctgaa
     1441 agcttccccg ataaattgat gcccgtctgt attttcaccg gcgattgcca cgaacattga
     1501 cccgctgatc gcctggcgac tgtcaacaac cgcttcgctg aaaaaacggt ttttttctgc
     1561 caactgttgt ccggttaatg cttccaagat ttcattgagt gaaaacactt tattccctta
     1621 tccaattccc gggttatccc gtttgtctgt tatcggccga acccccaatc ctttcggtaa
     1681 ctgcgaaatc atcaataata ctcatttttc ggtacttcgg tcggacaggt ttttgaatcc
     1741 aaacatgccc gttggcggtc gtaaggaata tgcaaatacg gcattatttc attcatgaca
     1801 tcactgaata aaggcgccga aacttcggat ccccaaatac tgatcaaagg ttcctgcagc
     1861 catacgtaag cgatgaattg cgggtcatct gtcggacccc agccgacgaa agatgcatta
     1921 gtgacattca ggacgtaccc atagccctct ttcgcaatct ctccggttcc ggtttttccc
     1981 gcaatcttta ctccatcgag gagagcgtcg gaagcttcca cttccagaga tgtctcaagc
     2041 atatccgtaa ccgtatgcgc cgtttcggca gaaatcggtc gccccactac ttcaggttga
     2101 atcacttcgg tatgatcgtt gtaataaatc gctttgacaa tatgcggttt catcattatt
     2161 ccgtcgttcg caatcgcact cactgcctgg atcatctgaa tcggagtcgt catcaaaccc
     2221 tgaccgaatg agttggtcgg aaggctgatc tccgtccagc cctcgtctcc gggttggaga
     2281 atcggataat aatcttccag tgcgagttcg atgccggtag gtttatccag atgaaacttg
     2341 cgaagatagt cataaaaaac cggagcggtg atctgttcgg caatccatga taaacatgtg
     2401 tttaatgaat gttgcataca gccgatcatt gtttgcggac cccaggcgcc ccgatcccag
     2461 ttataaatat caatccccat cacattgaag gtaccggtat cgttgaaaac ggtttccggt
     2521 ttgatgacac ccgagtcgat cccgatggct aatgtaataa ccttaaatac cgagcccacc
     2581 tcatacggct gcatcaccgc cgggttgaat ttattttctc tcgtaaaagt tgtggcggat
     2641 tcccagtaat ggttgagatt gatccgcgga taggtcgcca ttgccaggat ttcaccgttc
     2701 ttgggattgg cgataataat cgttccgctc tttgcttttt gttgctccac cgccaattta
     2761 acttgctctt ccactaattt ctgaacttta cggtcgatcg tcaatgcgat cgatgcgcct
     2821 ttgggcagat ccggaatgct ttccggaaca ttgggatcca aagagaattt tttctgaatg
     2881 gtttcaccgg ataaaatatc gtcgtaatat tgttcgatgc cgaaagaagc gccgctggcg
     2941 ggattcaaat acgggtaaaa accgataata ttggatgcca aatctccatc cgggtaatat
     3001 cggtaaatat tcggataata caccaccgca ttcaaatttt cgaaaacgcg tgttttactc
     3061 tcgaagctgg ttgtattccg ctcattcaga gatttttttg ccaattcgat tttatccact
     3121 tgttctttgg tagcaaaatt ggtaagcgga acttcaaccg cttcattctc gacatacggt
     3181 gtttcagcag cctgaataat cttttcccga tcgataccga gaatcggcgc aagatagttt
     3241 gcgataaatt caccgtgcgt attagaagct tgtaatttta gtgaaatcgt gtaagaaatg
     3301 gaatttccag ctaacaaata accttcccga tcgtagatat taccacgttc ggagcttact
     3361 gacaacgtcg tatattcata tgccttgccg atcgtttcct ttaattcaac cgcactggca
     3421 ttcgtctgaa gaaataatat acgcccgatt accaaaaccg caaaaatact taaacaaatc
     3481 gctaattttt taaatttgga gtcgatattc gagttcattt ttcactctcc tttgtcggca
     3541 atttccggaa tatatattgc tgaaggcttt cggtatattc gggcttcagc ggagataccg
     3601 gcaacggagg aagctgaact cttctttccg aaagaatgtc gatatcgggt atatatccgg
     3661 gaatagggag ataggtataa atgtcttcat catcgaaatc aattttgata tagcctgctt
     3721 gggtggcttt tttctccatc aatgcataag aagtgaggat tccttcccga gtcacataat
     3781 ccgcaatggt tcgggtgagt tcgtttcttt ggtcatgtaa tatttggatt tgcagtttca
     3841 tatccgtcat ttgtgccgaa taatttaaat aaaccgatgc gatacaggcg ctcaaaacca
     3901 ataccaacag gatattgatg atggatttca gctgttttcg ccaaggtgcc aatcgatatg
     3961 catgctgttc ggttttcatt tttctgtctc gattttttcg accactctta attttgcact
     4021 tcgcgctctc gggttttcac ggatttcgtc atcttttgca gtaatcgcat gacgatttat
     4081 ccgttttaaa cttgccttat gaccgcaggt acaaaccact tgttcaggcg gacagagaca
     4141 gtccgtactt tcgatgcgaa aaaagttctt tacgatccga tcttccaaag aatgaaaaga
     4201 aatgaccgct aacctgccgc ccggtttcaa caatgcggtc aaaataggga gcgctttttc
     4261 gatggtttcg agttcgttat ttacggcgat acgcagtgct tggaaacttt ttgtcgccgg
     4321 attatttttg gattcccgat tggtatgcac ggcattcgca acgatttccg caagcatcgt
     4381 cgtagtctcg atcggttctt ttacacgtgc actgcaaatt gccgcggcga ttcttcttgc
     4441 ggctctttct tcgccgtatt cccaaaaaat acgtgaaagt tcttcttcgg atgcgaaatt
     4501 gactaaatcg taagcagagg tcttctgatt cggatcaaac cgcatatcca gcggtccttc
     4561 cttttgaaaa gaaaatcctt tttccgctgc gtccacttgc atcgaagaaa tgcccaaatc
     4621 aagcaagatt ccgtcgaacc ccgttatatt atcgaaacag gacgtgatat atcggtccgc
     4681 aaacgcatag gatccccgga taattttgac ccgttctccg aattttgcca atcgttttgc
     4741 cgccatttcg atcgcgtttg gatctaagtc cattccgatc agctttcccg acggagcgga
     4801 tgcgtttaat atcgcttcgc tatgccctcc cgccccgagt gttccgtcca gataccattt
     4861 tcccggttcc ggtcgcagtg cctcaacgac ttcgttgagt aaaacgggtt ggtggcttaa
     4921 agtttcctgc gtcatatttt gttactcatc agacgggcaa attcagtcgt taccgaatcg
     4981 tcatggaaaa cttcttcttc ggctttcttc cattcatcta aagtccagat ctcgatatat
     5041 tcgtccgagc cggcaaaaat gattttgtcg ccatcctgga aattaaattc atcccgtaaa
     5101 aatggaggaa tcagaaatct tccgttcgcg tcaaattcga ttggttccgc tttattgaac
     5161 attcgccggc gatattttcg ggcgaccgga tcggccaaat taaatgaact gatttcattc
     5221 gccaactttt caaagtaagg caaagggtaa atgaccaaat ttttatcgat tcccacggaa
     5281 atataagcac cctctgccag caaatttcta aatcgtgccg gcagacaggt tctgcctttt
     5341 ttatcgaccg tgtgttcatg tattccggtg aacatatttt ctattttgtc ctttaaaaac
     5401 agaagactgg aaaaccagtc tgccacttta tgggaatcta taccacttta tgttccatta
     5461 taacaacctg gcttaaaaat tgcaatagat aaatccggat tttctaacca attttcaaaa
     5521 ttggtttatt tttggtttaa ttaccggtcc gatttttggg tttaatgtgt tttttccctt
     5581 ctttcgtttc aattgttcca ataacccgcg ataaggtgta agggttcaat cgcaaaataa
     5641 tcggaaaacc gccct
//
LOCUS       DAOMIV010000072         5655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14467_length_5655_cov_4.386964, whole genome shotgun sequence.
ACCESSION   DAOMIV010000072 DAOMIV010000000
VERSION     DAOMIV010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14467_length_5655_cov_4.386964"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKV59_02760"
     CDS             complement(<1..751)
                     /locus_tag="PKV59_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45704.1"
                     /translation="MLDFDDKELLEGFEEEFKKKKRNKRSGNRFPSWIIIAIILVAGV
                     FFFLYKKPDTVKVQTNKTPTVELTEGQQSLIQTRQISSSTVLSAEEGKTIETVSTARI
                     KGSSDKLTPTPSTSMPSEESEKLTAGTDIAVTGSALIQSTEAVVHNTTSGQLTIADTA
                     IPVVSAPAIRDSQPTENATGVIKETERSEITAEKTESLKITSTTGVESEKASYNLNFF
                     QKILFFLNPNWVSVPSITPTVAVPLKITEENP"
     gene            complement(766..1842)
                     /locus_tag="PKV59_02765"
     CDS             complement(766..1842)
                     /locus_tag="PKV59_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45705.1"
                     /translation="MPEEAVHIISSGTVRCARLLSEDGTVVVFKGQMADPTTQIAFRK
                     IWSRHFSLNILDALDLDPADNIREFLTIKEGDFVSVGEQIAKREGRRKLSVTAKASGR
                     FLGISANRLIFESDPVDIESRVAGFPGVVTDIVNNRGAYLETSGSYIKGLWGNGKCGQ
                     GILLSIDMERNNGIFDLDSISVDLAGAVVFANTCTDANVLKTAAKMTPGGLIFGSLPS
                     YLLPVAVSLPFPIIVTDWIGTGKISNPVHLTLGENIIKFAYLYSDNSASGKLIRPEII
                     IPQDEYIPEKENPLNHLGIGSQVRIIEGFYGGELGIVVDILPGNDENSDNMEIAPDQV
                     KICIDEETYVTLPKNNVEVINLKS"
     gene            complement(1835..3595)
                     /locus_tag="PKV59_02770"
     CDS             complement(1835..3595)
                     /locus_tag="PKV59_02770"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HQP45706.1"
                     /translation="MNRNQDIHTVLYVDIGSVNTRVCLFDRMDDQYSFIGAASATTSG
                     DITNQFEYLNVIQAVEKLEHATKRKILDRNQNIVMPATLDHSGIDQVAVSYTCIPDPR
                     IAVMGLTHAGSLQNVYDLLSKVGVKPVAEICAEDGKTVCENLDLFLRTAPRIVIVSGG
                     TEQGAEIGIYRLGEILMLACRSLPKEQRPIILYAGNSAAKKQMERVFSQITDISFSTN
                     VLESGSGVQPTLNGLINVLRKDLESRAPGFRILREKTKCSVIPGEFAFGRTIRLMSRL
                     IRQNRHILGIDIGAHQTILADAVNLALSLHTLPLGIGNNIGNLLNEYTPSDLQSWISF
                     QIKSAEIQDYILNKSYYPDLIPANKYSAEIELALARAIVMKGIENESLSAMLSDGSLA
                     MVFLSGAVLRNVEDPGEALMFAMDSLAPVGMVDYLLDMNGLSGGIGTLAHLNPVLASQ
                     IMSTSAYLNLGKVIRPSVRGEPPGKSIMTVSVRDDAGNTRKYDIPFNRIYRIPLEYGK
                     NYELEWTKISHNVTVPGVKTWAPAGFKSGCFGLVFDTREEKDGKIKLPKEKAARDKKL
                     QSWRTELGPWLVKQEEESHA"
     gene            complement(3597..4295)
                     /locus_tag="PKV59_02775"
     CDS             complement(3597..4295)
                     /locus_tag="PKV59_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45707.1"
                     /translation="MTSPSDFTVLLIGFLLSVFTFSLILGDNWLFRLSSAVLSGTLAG
                     YLINVLIEKIFIPSIVNPLMEPNVDWKNQALAVVVIISAVFLFLKYYFRSPAGGNAIL
                     AVLLCSAAAITILGIVNGTLINLYRGLIARFLPGAEMSSANIYWVKTGIITLGVISSL
                     IYTQHYAFGKKNGKEISRQRNTVFFFRTLGEVFIGIAMGAVFAGCFIASSTILIGQIS
                     NLVGSGQEILQWVK"
     gene            complement(4279..>5655)
                     /locus_tag="PKV59_02780"
     CDS             complement(4279..>5655)
                     /locus_tag="PKV59_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45708.1"
                     /translation="VDSFLRQIAAGTETPQEPIQESAAMTSPEWVTSPEPAPQPSSGT
                     DEELEFLMENPAPSAEEVREEAVTGQNQEEPSVSESVPEEPEPPVSSEGWTQDEIVPD
                     ELEFEEDDLDEVPWNLFETGEMSLPGLEPAEIAYKPFSKSGIQEGKNNQDYQQRMVMT
                     ILDKIFQIEGLNKPFVKNQNRGTRKNTQFLIGLVMILGILAILFTGITDSIPLNPPDP
                     ASFPNLVTFNREVDKLTNNDHVLVVVDYIPGFENEMNEPVTALLKNITDKKARISLVT
                     TNLSSAAVTAKLKAEIDPTGIEDIGYRTGGVLAIQSVLQNDLSPYTTVYLVSSGFESA
                     RTWLEQIASMTVPLPVNVIASTQLESLLQPYYESKLINSYLTGKMEKEIFSGFTFDDP
                     IRNRRLFALWFLIFTLILAFLAGNVQRTPYHTIIVEEKPKSRPPKPSTSDKTENKIDK
                     GIKNDIAF"
BASE COUNT         1465 a         1350 c         1271 g         1569 t
ORIGIN      
        1 acggattttc ttcggtaatt ttcagcggta cggcgacagt gggagttatc gaaggaaccg
       61 aaacccagtt tggattcagg aaaaacaaaa tcttctggaa aaaattcaga ttgtaagatg
      121 ctttttcgga ttccacccct gttgtcgagg tgattttcaa agattcggtc ttttctgcgg
      181 taatttcaga acgctccgtt tcttttatta caccggtcgc attttccgtc ggctgtgaat
      241 ctcgaatcgc gggagccgaa acgacgggga tagccgtatc ggcaattgtc aattgacccg
      301 aagtcgtatt atgaacaacc gcctcggtgc tttgaatcaa ggcggaaccc gttacggcga
      361 tatcggtacc ggctgtgagt ttttccgatt cctcagatgg catcgaagta gatggtgtcg
      421 gagtcaactt gtcggaagat cccttaatcc gggcggtaga aaccgtttcg atcgtttttc
      481 cttcttccgc ggataaaacg gttgagctgc ttatctgccg agtctgaata agggattgtt
      541 gaccttctgt taattcaact gtcggggttt tgtttgtttg gaccttcacg gtgtccggtt
      601 ttttatataa aaagaagaaa acaccggcga caaggattat agcgataatg atccatgacg
      661 ggaatctgtt ccccgatcgc ttgttgcgtt tcttcttttt gaactcttct tcaaagcctt
      721 ccaataattc cttatcgtcg aaatccagca tgaggatccc cttttctaac ttttcaaatt
      781 gattacttcg acattgtttt tcggcaaagt aacatatgtc tcttcatcaa tgcagatttt
      841 cacctgatcc ggtgcgattt ccatattatc tgaattctca tcgtttcccg gcagaatgtc
      901 aaccacgatt cccaactctc cgccataaaa accttcaata attcgaactt gcgatccgat
      961 tcccaaatga ttgagcgggt tttccttttc cggaatatat tcatcctgcg gtatgatgat
     1021 ttccggtcga atcaactttc ccgaggcgct gttgtccgaa tataaataag caaatttaat
     1081 gatattttct cccagcgtga ggtggacggg gttgcttatt ttcccggttc cgatccaatc
     1141 cgttacaata atcggaaaag gcaggcttac cgcaaccggt aacaaatagg atggcaacga
     1201 tccgaaaatc agtccgccgg gggtcatttt ggcggcagtt tttaagacat tcgcatcggt
     1261 gcaggtattc gcaaaaacaa cggctccggc aaggtccaca gaaatgctgt ccaaatcaaa
     1321 aatgccgtta tttctttcca tatcgatgga aagaagaatg ccctgtccgc atttcccgtt
     1381 tccccacaaa cccttaatat aacttccgct tgtttcaagg taagctcccc gattattgac
     1441 gatatccgtc accacacccg ggaatcccgc aacccgcgat tcgatgtcaa cgggatcgct
     1501 ttcaaagatc aatcgattcg cggaaattcc caaaaaacga ccggatgcct tcgctgtgac
     1561 ggataatttc cttctgcctt cccgtttcgc gatttgttct ccgacggata caaaatcccc
     1621 ttccttaatc gtcaaaaact cacgaatgtt atccgccgga tctaaatcca atgcatccag
     1681 aatattgaga gaaaaatgtc ggctccaaat ttttctgaag gcgatttggg ttgtcggatc
     1741 ggccatttgc cccttgaata ccacaacggt gccgtcttcg gaaagcaatc gggcacagcg
     1801 caccgtaccg gacgagatta tatgaaccgc ttcctcaggc atggctttct tcctcctgtt
     1861 taaccagcca gggacccagt tcggtccgcc acgattggag tttcttgtca cgggcggctt
     1921 tctctttcgg cagtttaatt ttcccgtctt tttcttcccg agtatcgaaa accaacccga
     1981 aacagccgct tttaaaaccg gcgggagccc aggtcttaac acccggaaca gtgacgttat
     2041 gtgatatttt tgtccattcc aattcatagt tttttccgta ttccagcgga atccgataaa
     2101 ttcgattaaa gggaatgtca tattttcgtg tatttccggc atcatcacgg acggaaacag
     2161 tcattatgga tttgcccggc ggttcacccc gtacggaagg acgaatgact tttccaagat
     2221 tgaggtaggc ggatgtgctc atgatttggg aagctaacac aggattcaag tgcgccagcg
     2281 taccgatccc acccgacagc ccgttcatat caagcagata atcgaccatt cctacgggtg
     2341 ccaagctatc catagcaaac attaatgcct ctccggggtc ttcgacattg cgtaagaccg
     2401 cgccgctgag aaataccatc gccaacgatc cgtcagaaag catggcgctc agggattcat
     2461 tctcaatacc cttcataaca attgcgcggg ctaaagccag ttcgatttcc gccgaatatt
     2521 tgttggcggg gatcagatcg ggataataac ttttattcag aatataatct tgaatctcgg
     2581 cgcttttgat ttgaaaactg atccacgatt gcaaatcgga cggtgtatat tcgttcaaga
     2641 gatttccgat attattcccg atgcccagcg gaagagtgtg taacgagaga gccaggttaa
     2701 ccgcatcggc caaaatcgtt tgatgcgcac cgatatcaat tcctaagatg tgccggtttt
     2761 gacgaatcaa ccggctcatt aaacggatcg ttcgtccgaa agcgaattct cccggaatga
     2821 cggagcactt ggttttttcc cgtagaatcc gaaaaccggg cgcacgtgat tccaaatctt
     2881 ttctcaatac attgatcaag ccgttcagcg tcggttgaac accggagccg gattccagta
     2941 cattggtcga aaatgagata tcggtgatct gtgaaaagac ccgttccatt tgttttttcg
     3001 cagccgaatt ccccgcatag aggatgatcg ggcgttgttc tttcggcaaa cttctgcatg
     3061 ccagcatcag gatttcgccc aagcggtata taccgatttc tgcgccttgt tccgttccgc
     3121 cgctgacaat gacaatccga ggtgcggtac gcaagaacag gtctaagttt tcacaaacgg
     3181 ttttaccgtc ttcggcacaa atttccgcaa ccggcttcac tccgactttc gaaagcaaat
     3241 cataaacatt ttgcaggctt cccgcatgtg tgagacccat aacagcgata cgcggatcgg
     3301 gaatgcacgt ataagaaacc gcgacttgat caatgccaga atggtcgagg gtagccggca
     3361 tcacaatatt ctgattacgg tccaaaatct ttcgtttggt ggcatgttcc agcttttcaa
     3421 ccgcttggat cacgttcaga tattcaaact gatttgttat atctccggaa gttgtagcgg
     3481 atgccgcccc gataaaacta tattgatcat ccatccgatc aaataagcaa acacgcgtgt
     3541 tgacggaacc gatatcgaca tataacacgg tatgaatatc ctgattccgg ttcatcttat
     3601 ttcacccatt gcagtatttc ctgaccggat ccgacgagat ttgaaatttg accgatcaaa
     3661 attgttgatg aggcgataaa acagcctgcg aaaacggcac ccatcgctat tccgataaaa
     3721 acttctccca gggttctgaa aaagaaaaca gtattccttt gtcgactgat ttcctttccg
     3781 ttttttttgc cgaaagcata gtgttgggta taaattaacg aagaaatgac acccaaagta
     3841 attatccctg tttttaccca gtatatattg gcggaactca tttctgcacc gggaagaaaa
     3901 cgagcgatca agccgcgata aagattgata agagttccgt taacaattcc gagaatggtt
     3961 atggcagccg cgctgcataa caaaactgcc aaaatcgcat ttccgcccgc gggggagcgg
     4021 aaataatact tcaaaaacag aaatacggca gatatgatta caacgacggc cagcgcttgg
     4081 tttttccaat cgacgttcgg ttccattaat ggatttacga tcgatggaat aaaaattttt
     4141 tcgatcaata cgttaatcag atagcctgcc aacgtcccgg acaatacagc tgaagaaagg
     4201 cgaaacaacc agttgtcacc cagtatcagg ctgaaagtaa aaacggataa aaggaatccg
     4261 atgagcagaa cggtgaaatc agaaggcgat gtcatttttg attcctttat cgattttgtt
     4321 ttctgttttg tccgatgtcg acggtttcgg cggtcgcgat tttggttttt cttcgacaat
     4381 gattgtatga tacggtgttc gttgaacgtt tcccgcgagg aatgccagga ttaaagtgaa
     4441 aattaaaaac caaagagcga ataatctgcg atttcggatc ggatcatcga aagtgaagcc
     4501 gctgaaaatt tccttctcca tcttcccggt cagataactg ttgatcaact tgctttcgta
     4561 atagggctgc aataaagatt ccaactgcgt cgacgcgatg acattgacgg gcaacggaac
     4621 ggtcatcgaa gcgatttgct ccagccaggt tcttgccgat tcaaatccgg aactgaccaa
     4681 ataaacggtt gtgtaaggag agagatcatt ttgcaggacg gattggatcg ccaaaacacc
     4741 gccggtccgg tagccgatat cctcgatccc ggtcggatcg atttccgctt tcagctttgc
     4801 cgtgacagcc gccgaagaaa gattggtcgt cacgaggctg atccgcgcct ttttatcggt
     4861 gatatttttc aacagtgcgg taaccggttc gttcatctcg ttttcaaagc cgggaatgta
     4921 atcgacgacg accaaaacat gatcattgtt tgtcagtttg tccacctccc gattaaaagt
     4981 caccaaattg gggaaagacg ccggatcggg tggattcaat gggatggaat cggttattcc
     5041 ggtaaacaaa atggcaagaa tacccagaat catgaccaat ccgatcaaaa attgtgtatt
     5101 tttccgagtt ccgcggtttt gatttttaac gaacggttta tttaaacctt cgatttggaa
     5161 gatcttatcc agaatggtca taaccattct ttgttgataa tcttgattgt tttttccttc
     5221 ttgtatgccc gatttggaga aaggtttgta ggctatctcg gccggttcaa gcccgggtaa
     5281 ggacatttca ccggtttcaa ataaattcca cgggacctca tccaaatcgt cttcttcaaa
     5341 ttccaattca tcgggaacga tttcgtcctg tgtccaccct tcagaggaaa cgggtggctc
     5401 aggctcttcc ggaacggatt cggagacgga tggttcttct tgattctgtc cggttaccgc
     5461 ttcttctcgg acttcttcgg cggacggcgc cgggttctcc attaaaaatt ccaactcttc
     5521 atctgtgccg gaagacggct gcggagccgg ttcgggcgat gtaacccatt cgggcgaagt
     5581 catcgcagcg gattcttgaa tgggttcctg cggcgtttcc gtaccggcgg ctatttgtct
     5641 taaaaatgaa tccac
//
LOCUS       DAOMIV010000073         5637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14526_length_5637_cov_4.804550, whole genome shotgun sequence.
ACCESSION   DAOMIV010000073 DAOMIV010000000
VERSION     DAOMIV010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14526_length_5637_cov_4.804550"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PKV59_02785"
     CDS             complement(<1..504)
                     /locus_tag="PKV59_02785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HQP45709.1"
                     /translation="MNNTALIIVDMVKDFTDPGGLVYYPQNREILPKIVAVLEESRNF
                     GKLIIFLQHCYRKGKPDKNLRTMRPNCIEGTDGDQIDPMLRVDPIKDYVIRKRRYSGF
                     FGTDLDLVLRENEIKNTVIVGTKTNCCIRATVTDAYNLDYNAIVIRDCVATDSEVVNQ
                     VHLSDIEK"
     gene            complement(497..1414)
                     /locus_tag="PKV59_02790"
     CDS             complement(497..1414)
                     /locus_tag="PKV59_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45710.1"
                     /translation="MDLLQSILYDMVYHSTPIILCVIGGVFAYKANVLNIALEGMMLC
                     GAFIVMLVDFLTGNLALAVSASILSVLLLGLIFSFFGITCKGNVIVVGLGINMLVSAI
                     AGFVLKVMNSSNILINSLKVSDFKINIPVIENIPILGSLLSGHPLITYLSFLGIFLMW
                     VLMYKTKFGIYVRVVGENEDAARSLGLKVSFYRYMAILIGAFCCALAGINLSLERLGL
                     FTNDMTAGRGFVAIAAIYCGHGDPLVSAVYAIIFGLARSLSINLSIFAGPAAGLFDVI
                     PYVLMTIVLTVVSIYRYKNIKIRGFRINE"
     gene            complement(1414..2511)
                     /locus_tag="PKV59_02795"
     CDS             complement(1414..2511)
                     /locus_tag="PKV59_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45711.1"
                     /translation="MNRKQKWASFGRSLLSSLVPVIAAFIIGGIIISLLGENPFATYW
                     ILISKSLFTVKGFAYTLHKAAPLILTGLAIAVTFKANIFNMGVEGQLLIGGFFAGIAG
                     ASLTGTSPAVTKIICMAVGMGCGVLFALIPAVLKAYFRVNEMVVTLMLNYAMIKILEF
                     LATGVFRDNGSGYVTTPTIQAGAMFRRLGGSKLTPFFFISLIVFLIMYFVMNRSVLGY
                     EIRAIGLNPVFAEAASMKVRNKIIEIMVISGALSGLAGAGQMMSDQFKYTLTFSGTPG
                     LGWDGMLVSLLGKHDPVGILIAAIFYSALKTGSDKINFYTNVPKEIIAVIQSLIILFL
                     AVQFFDEKTNLFDSLKKKITGRDETTVKEEL"
     gene            complement(2520..4088)
                     /locus_tag="PKV59_02800"
     CDS             complement(2520..4088)
                     /locus_tag="PKV59_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45712.1"
                     /translation="MSIIRTENLTKKFGDFVANDHINISVEENEIKCIVGENGAGKTT
                     LMNMLYGLLRPTSGKIFIREKEVKINSPLDAISLGLGMVHQHFKLVPSLTVYENIFLG
                     TEIEKKTAKGLRLPFLDEKKEIEEVQKLIDRYKFELNPLDKIEDISVGERQRVEILKM
                     LYRNVDILILDEPTAVLTPQEADDLIASLKELRKQGKTIIEITHKLREVMELSDSVTV
                     MKLGKVIGNVKTSETNEQELAQMMVGRDVVLSVANKHKTGGSQKEIYRVNNLCTINEY
                     GKEVLHNISFSLKTGEILGIAGVEGNGQTELVNVLTGVMESTSGTVIANGNDITNKWP
                     DELRNNGIAIIPEDRFAQGLCRGMDLAQNCIAGYHRRPDVCSRGLLKREVMEEKRDRM
                     IRDFDIRVADPNGLIDQLSGGNAQKVIVAREFESKPQILIACQPTRGVDVGSIEFIHT
                     QLLKFRDAGNAVLLISSELSEIMSLSDRVLVMYKGAIIGEIDPQHTTPEEVGLLMAGI
                     EIKPVSAEQKVKQD"
     gene            complement(4198..5349)
                     /locus_tag="PKV59_02805"
     CDS             complement(4198..5349)
                     /locus_tag="PKV59_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP45713.1"
                     /translation="MKKKLWVFVSLVLIAMFAMTGAVSASDLKIGIVCSAAGQNDNGY
                     NQSAIEGAKQVSEELGIDYKVIDATSDVPAALETLAEDGYNLIFSLEYDFEALIKGVG
                     GNKPIAEQYPDTTFVVFNDNPNIDENGNVIHKNVISVLFDVHEASFVAGALSVLVNEN
                     NAVLFGEGYHFTDPAKTRAIGFIGGTNSNGITVFSYGFIQGINYVAQQLGVKYDYYAK
                     YDAGFSDPATGSTVAGTYYDNGANVVFGCAGNVGDGITSKAKEVGKLAIQVDANKDNQ
                     QPGYVLTSVLKNTKVPVYTISKALKDGTLSSMGNLQTYSLSSGATGITDLSVIGEHIQ
                     KTDAAAAKWAEIKQQVSDISAKIGSDIKVVNAAIGEKFDPASCPNVVIK"
BASE COUNT         1618 a         1399 c         1069 g         1551 t
ORIGIN      
        1 tttttcgatg tcagagagat gcacttggtt aacaacctct gaatcggttg ccacgcaatc
       61 acgaatcaca atggcgttgt aatcaagatt gtatgcatcc gtaaccgttg ctcgaataca
      121 gcagtttgtt ttggttccga cgatcaccgt attttttatt tcattttcac gtaaaacaag
      181 atccagatca gttccgaaaa aaccgctgta acgtcgtttg cgaataacat aatccttgat
      241 cggatccacc cgaagcatcg gatcaatctg atcgccgtcc gtcccttcga tacaattggg
      301 gcgcatcgtt cgcagatttt tatccggttt cccttttcga tagcaatgtt gcaaaaatat
      361 gatcaatttt ccgaaatttc gactctcttc aagaacggcg acaattttcg gaaggatttc
      421 ccggttctgc gggtaataaa ccaacccgcc gggatcggta aaatctttga ccatatctac
      481 aattatcagt gcggtattat tcattgatcc gaaagcctct tatcttaata ttcttataac
      541 gataaatcga cacgaccgtc aaaacaatgg tcatcagaac atagggaatg acatcaaata
      601 agccggcagc cggtccggca aaaatgctca gattgataga gagcgaacgt gccaagccga
      661 aaataatggc atagacagcc gaaacgagag gatcgccatg cccgcaataa attgccgcga
      721 tcgctacaaa accgcgtccg gcagtcatat cattcgtaaa taaacccaac ctttccaaag
      781 aaaggttaat gccggcaaga gcgcaacaaa aagcaccgat caggatagcc atatagcgat
      841 agaaggatac tttcaacccc aggctgcgag ccgcatcttc gttttcaccg accacacgga
      901 cataaatgcc gaatttcgtc ttatacatca gcacccacat caaaaaaatg ccgagaaagc
      961 taaggtaggt aatcagcgga tgaccgctca gaaggctgcc gagaatcggg atattctcga
     1021 tgaccgggat attgatttta aagtcactta ctttcagcga attaatcaga atattcgaag
     1081 aattcatcac ctttaataca aagccggcta tcgccgatac caacatatta atacctaaac
     1141 cgaccacaat cacatttcct ttgcaggtaa ttccgaagaa ggaaaaaatc aatcccagta
     1201 acaataccga taaaatcgaa gcgctaacgg caagtgccag atttccggtc agaaaatcaa
     1261 ccaacatcac tataaaagcg ccgcagagca tcataccttc caacgcaatg ttgagtacat
     1321 tcgctttata ggcgaataca ccgccgatta cgcaaagaat gatcggcgta ctgtgataga
     1381 ccatgtcata aagaatactt tgcaggagat ccattacagt tcctccttca cagtcgtttc
     1441 atctcttccg gttatttttt tcttcagact gtcaaataaa ttcgtctttt catcaaagaa
     1501 ttgaacagcc aaaaaaagga taatcaggct ttgaatgacc gctataattt cttttggaac
     1561 attggtatag aaatttattt tgtcgctacc ggtcttcagc gcactgtaaa aaatcgcggc
     1621 aatcaaaatt ccgacgggat catgtttgcc cagcaaagaa accaacatac catcccaacc
     1681 gaggccgggc gtgccggaaa aggtcagtgt atatttgaac tgatcactca tcatttggcc
     1741 tgcgcctgcc aatccggata atgcgccgct gataaccata atctctataa tcttattgcg
     1801 gactttcatg cttgccgctt ccgcaaacac agggttcaat ccgatagctc tgatttcata
     1861 cccgagtacc gaacgattca tcacaaaata catgattaaa aaaacaatca acgaaataaa
     1921 gaaaaatggc gtcaacttcg agccgccgag gcgccggaac atagccccgg cctggattgt
     1981 tggcgttgtc acatatccgg acccgttatc tcggaaaaca cctgtcgcaa gaaattcgag
     2041 aatttttatc atggcatagt tcagcattaa tgtcacaacc atttcattga ctctgaaata
     2101 tgcttttaaa accgccggaa tcaacgcaaa aaggacaccg cagcccattc cgaccgccat
     2161 gcagataatc tttgtgacgg cgggagatgt gccggtcaaa gaagcgccgg caataccggc
     2221 aaaaaaaccg ccgatcagca attgtccctc cacgcccata ttaaagatat tcgccttgaa
     2281 ggttaccgca atagccaagc ccgtcaaaat caaaggggca gctttatgca gcgtataagc
     2341 aaagcccttg acagtaaaca gcgatttgga aattaaaatc caataggtag caaaaggatt
     2401 ttcaccgagt agggagataa taatccctcc gattataaat gcggcaatga ccggcaccag
     2461 tgagctgagc aacgatctac caaaagaagc ccatttttgt ttccgattca ttgattccat
     2521 taatcttgtt tcactttctg ttccgcactg actggtttga tctcaattcc cgccatcaac
     2581 aacccgactt cctccggtgt ggtatgctga ggatcgatct caccgataat tgccccttta
     2641 tacatcacga gaacccgatc ggaaagactc ataatctccg aaagttcgct cgagatcaac
     2701 aggaccgcgt ttccggcatc ccgaaatttc aacagttgag tatgaataaa ttcaatcgaa
     2761 ccgacatcca cgccacgtgt cggctggcag gcaatcaaaa tctgaggctt actttcgaat
     2821 tctctggcga caatgacttt ctgagcattg cctccggata attgatcgat gaggccgttg
     2881 ggatcagcga ctcgaatatc aaaatctctg atcatgcgat cgcgcttctc ttccataacc
     2941 tctcgcttta ataatcctct cgagcagaca tccggacgac ggtggtatcc cgcaatgcaa
     3001 ttttgcgcaa ggtccattcc acgacaaagt ccttgagcaa aacgatcttc cggaataatg
     3061 gcaatcccgt tatttcgcaa ctcatcgggc catttattgg taatgtcatt accgtttgct
     3121 ataacagtac ctgaagtact ttccatcaca cccgtcaata cattcaccaa ttcggtctgg
     3181 ccgtttccct ccacaccggc aatgccgaga atttctcctg ttttcaagga gaaactgata
     3241 ttatgaagca cttccttgcc atattcattt atggtgcaaa gattgtttac gcgatagatc
     3301 tctttctgac tgccgccggt tttatgcttg tttgctaccg aaagaaccac atcacgaccc
     3361 accatcatct gagcaagttc ctgctcattt gtttcggaag tttttacgtt accgatcact
     3421 ttaccgagtt tcatcaccgt cacactgtct gaaagttcca ttacttcacg aagtttgtgt
     3481 gtaatttcaa tgattgtttt gccttgtttc cgtaattctt ttaaactggc aatcagatca
     3541 tcagcctctt gtggcgtcag aacggctgtc ggttcgtcca gaataagaat atctacgttt
     3601 cgataaagca tcttcaaaat ttcaacccgt tgtctttcgc caacggagat atcttcaatt
     3661 ttgtcaagag gattcagttc aaatttatat cgatcaatga gtttttgaac ttcctctatt
     3721 tccttttttt catccaaaaa aggtaagcgt agaccttttg ccgtcttttt ttctatttct
     3781 gttcccaaaa aaatattttc ataaaccgtc aagctcggga ccagtttgaa atgctggtga
     3841 accatcccga gccccaagga tatcgcatcc aaaggactgt ttattttgac ttccttttca
     3901 cgaataaata ttttgccact ggtaggtcgt aataatccgt aaagcatatt cattagggtg
     3961 gttttcccag ccccgttttc tccgacgata cacttgattt cattttcttc cacggagata
     4021 ttgatatgat cattcgcgac aaaatcgcca aatttttttg tcaaattttc ggttcgaatt
     4081 atgctcattc tttcactttg tattcatatg gatcttatgg gacgattttc tttccaaaag
     4141 ggtgcccaaa gatctttggg cactcttttg tttcatcatc ttccttcgga ggaacaatta
     4201 tttgatcaca acattcgggc aggaagcggg atcaaacttt tcgccgatcg ccgcattgac
     4261 aaccttgata tcgctgccga tctttgcgga aatgtcactg acctgctgtt taatctcagc
     4321 ccatttagct gcggcagcat ccgtcttttg aatatgttcg ccgattactg acaagtccgt
     4381 aatacccgtc gcgccggagc tcaaagaata ggtctgcaaa tttcccatgc ttgaaagcgt
     4441 tccgtccttc agagctttcg aaatggtata aaccggcact tttgtatttt tcagaacact
     4501 ggtcagaaca taacccggct gctgattatc cttgtttgca tccacctgga tcgccaattt
     4561 tccgacttcc ttggcttttg aagtaatacc gtcaccgaca ttaccggcac acccgaaaac
     4621 aacattcgcg ccgttgtcat aataggtacc ggctaccgta ctgccggtcg cgggatcact
     4681 gaatccggca tcatatttgg cataataatc gtatttcaca cccaattgtt gagccacata
     4741 attgatgcct tggataaagc cataggagaa aacggtaatt ccgttcgaat tggttccacc
     4801 gataaagccg atggcacgcg ttttggcagg atcggtaaaa tgataccctt ctccgaacaa
     4861 aacagcatta ttttcgttga ctaaaacact caatgcaccg gcaacaaagg aagcttcatg
     4921 cacatcgaat aggacggaaa tgacattttt atgaatgaca ttcccatttt catcaatatt
     4981 cgggttgtcg ttgaaaacaa cgaaagtggt atccggatat tgttcggcga taggcttgtt
     5041 gccgccgacg cctttaatca aggcttcaaa atcatattcc agagaaaaaa tcagattata
     5101 gccgtcttcg gccagtgttt ccagagccgc cggtacatca ctggttgcat cgatcacttt
     5161 ataatcaatg cccaattctt cactgacctg tttcgcacct tcaatcgcag actggttata
     5221 accgttgtca ttctggcctg ccgcagaaca gacaattcct attttcagat ccgatgcgga
     5281 aaccgcaccc gtcatcgcaa acatagcgat gagaaccaaa gacacaaaca cccacaattt
     5341 tttcttcatg ccaatctcct tataataaaa tgatgatgga ttgcttattc acctcttttg
     5401 tccaagccat catatttttt ccgactttat tcaaaaccct tcaagaaatc tccgaattca
     5461 catcaatttt ccgatacctg atgaatataa aatttgaact tatcgctgcg ataatggcaa
     5521 cggaaagtct ccaccggctg ctcgatccca tccactttac cgtaggtagt acaggtaaac
     5581 aacagaatcg gaatatccgg cgtaattccc aacaaccgtg ccgtttcctc atccgaa
//
LOCUS       DAOMIV010000074         5550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_14830_length_5550_cov_4.328844, whole genome shotgun sequence.
ACCESSION   DAOMIV010000074 DAOMIV010000000
VERSION     DAOMIV010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5550
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14830_length_5550_cov_4.328844"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PKV59_02810"
     CDS             <1..521
                     /locus_tag="PKV59_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45714.1"
                     /translation="ETTYYLCVNDLVVEQTSGDIQKLKMPRFVTTRALKWLKPEENLF
                     FLYSTYTGPTFATDIRKRMWEGATVTYMALQTAFYLGFRQVILIGVDHNFQTKGKPNE
                     TVVSEGDDPNHFNPGYFGKGFRWQLPDLETSERAYTMAREAYQKAGREILDATIGGKL
                     QVFPKVAYDSLF"
     gene            528..1358
                     /locus_tag="PKV59_02815"
     CDS             528..1358
                     /locus_tag="PKV59_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQP45715.1"
                     /translation="MSHPHISVVTPSYNQAPFLEKTIQSVLTQKTPEIEYFVMDGGST
                     DGSIDIIRKYESEIDYWVSEKDRGQADGINKGFSLAGGEIFGWINSDDFYLKGAFEKV
                     LAYFDAHPEVDLVYGDVLSVNQEGELINVMRFADYALRDLMAFRIISQPAVFFRRKAW
                     YGAGGMDLRYHFLLDHHLWLRIAARGKIAYLPEPLAAARFYPEAKNRAHAADFGAEAY
                     RIADWLLTDPEMKPYSKGYRRVIQGGADWLNANYLSNGNHLGNLSRIMRKHLRNTLHG
                     "
     gene            1355..1486
                     /locus_tag="PKV59_02820"
     CDS             1355..1486
                     /locus_tag="PKV59_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45716.1"
                     /translation="MIEDRRRVGVTLLMAISPEKATDVFRREAQKRMDAFSTYRDLL"
     gene            1483..3000
                     /locus_tag="PKV59_02825"
     CDS             1483..3000
                     /locus_tag="PKV59_02825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP45717.1"
                     /translation="MNLHTIPSRKKLTGLAETVIVLILMAAGFAVRLYDLTDPPLDFY
                     SPRQLRSAIITRSFYYESLNNSDPTIVEQADKLADLERYEPPVMEWLVSKIDLLIGKE
                     VFWTGRIFSAFFWALGGLFLYRICRRFASFSAGIFSLLIYYFVPFSIIASRSFQPDPW
                     MCFWVLGTAWMLCNWSEKGSWSSAFFSGFIGGMALLVKIYAGFFVAFMLLGIFCASVN
                     RQNRKQRIFQTLLIGAVMLIPSAVYYLIFNPSRSGDFFSFWIVSLSGLIRTSNFYADW
                     IAMVKGLIGLVLPLFGLWGVLFIRNRQVKGLMIGWWLGYIFYGLFVPYQITTHEYYSL
                     ILIPLTAISVCPVSDAIFEKTRQSGKIAFVCSLLLIMAAAGYGAYSACGYLRGTNYVL
                     EPASWRKVGEAIPQNEPFIALTGDYGMRLIYYGWRQPSLTWPDAKDDTLFQLAGTPQA
                     DFDTYFESQTKGMNYFVVTALGELDNQPDLKKRLEFFPVYAAGNGYIIYDLRNKP"
     gene            2997..4514
                     /locus_tag="PKV59_02830"
     CDS             2997..4514
                     /locus_tag="PKV59_02830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP45718.1"
                     /translation="MRNIVARKKLSSIHIIIFILILLCGTFLRFVDFDDPPLDFHPAR
                     QFHSALIARSYFLKDNGNLPGKSADYQHEAEMRGSQEPRIEPPIMERLAAWGYKIVGD
                     ADLRVPRAMSIVFWLIGGIGLFRLCRRFLGITGSLVGFGFFLLFPYTIFASRSFQPDP
                     LMVALMIWSLNALVEWDSHPTIGSAILTGAVAGAAILVKQVCVFPLASAFAFYFFSSS
                     DWKQKIKRVDFWIIVALSVAPVLIYNYWGYFVKGFLVQQYQGRFSLSDWFSPAFYVRW
                     IRKMDEVFGIVVVTAGLVGCLACENRRARLLWIGFFAGYILYGLALPHHIGTHDYYQL
                     PLFPLISFGIGRSAESIFEVVGRLKEGRTLAYVGITVCLILLGAWWSADSVISLNRRD
                     YRAWPERWQSLAAELDPYPGQINTIGIMDDYGAAMIYWGLRTPVIWEQNIEKLSEEEA
                     DTTIRQTMINREYLIVTDLDSFYAQPRLQNWLVRNTKLYAEEPDYLIYDLRGLNE"
     gene            4507..5292
                     /locus_tag="PKV59_02835"
     CDS             4507..5292
                     /locus_tag="PKV59_02835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQP45719.1"
                     /translation="MSEKAVFPFVSIVTPSFNQAEFLERTIQSVLSQDYPNLEYIIID
                     GGSTDGSVEIIRKYENRLAYWVSEKDKGQTNAINKGFNRAKGEIFAWLNSDDIYEPGA
                     IQAAVEALMSDPSLGFVYGDCNFIDSHDRIIGKFDVRQTDLKKLQRGYVHIPQQAFFF
                     RGELWKKVGPLDESIYFAMDYDLWLRLARIAPFRYIPGKVWADFRLHEDAKTITADAS
                     CWPEMIQIRRRDGGSKFSVLFLKYQLRKLIAPLINYRRKKMFQ"
BASE COUNT         1378 a         1103 c         1409 g         1660 t
ORIGIN      
        1 ttgaaaccac ttattacctt tgtgtgaatg atctggttgt cgaacaaaca tccggcgata
       61 ttcagaaatt aaagatgccg cgttttgtca cgactcgggc actgaaatgg ctgaagcctg
      121 aagaaaacct cttttttctt tattcgactt ataccggccc gaccttcgcc accgatatcc
      181 gaaaaaggat gtgggaaggt gccacggtga cttatatggc gcttcagacg gctttttatc
      241 tgggattccg gcaagtgatt ttgatcggtg ttgatcacaa tttccaaacc aagggaaagc
      301 ccaacgagac ggttgtttcc gaaggagatg atccgaatca ctttaatccg ggttattttg
      361 ggaaaggctt ccgctggcaa ttaccggatt tggagacctc cgaacgtgcc tatactatgg
      421 cgcgtgaagc ctatcaaaag gcaggccggg aaatattgga tgctaccatc ggcggaaaat
      481 tacaggtttt tccaaaagtc gcttatgact ccctttttta aaaatcaatg agccatcctc
      541 atatttctgt tgtgacacca tcctacaacc aggcgccctt ccttgagaaa accattcaat
      601 ctgttttgac gcaaaagaca cctgaaatcg aatattttgt aatggatggg ggatcaacgg
      661 acggaagtat cgatattatt cgaaaatatg aatccgaaat cgattattgg gtatccgaaa
      721 aagatcgcgg acaggccgat ggaattaata agggcttttc attggcgggt ggagaaatat
      781 ttggttggat aaattccgac gatttttatc tgaaaggtgc ttttgaaaaa gtgctcgcct
      841 attttgatgc tcatccggag gtcgatttgg tatacggtga tgtactttcc gtaaatcaag
      901 agggagaact gataaatgtg atgcgctttg cggattatgc attgcgggat ttaatggctt
      961 tccgtatcat cagtcaaccg gcggttttct ttcgccgcaa agcttggtac ggcgcgggcg
     1021 gaatggattt acgctatcat tttcttttgg atcatcatct ttggcttcga atcgcagcga
     1081 gagggaaaat agcttatctg ccggaaccgt tggcggcggc ccgtttttac ccggaagcaa
     1141 aaaatcgcgc tcatgccgcg gatttcggag ccgaagctta tcgaatcgcc gattggttgc
     1201 tgacagatcc ggagatgaaa ccctattcga aaggatatcg acgggttatt caaggcggag
     1261 ccgattggtt gaatgcaaat tatctgtcta acgggaatca ccttggaaat ctctcaagaa
     1321 ttatgcgaaa gcatttgcga aatacccttc acgggtgatt gaagatcgtc gtcgagtggg
     1381 ggttacctta ttgatggcaa tcagcccgga aaaagcaacc gatgttttcc gccgggaagc
     1441 acaaaagcga atggatgctt tttccactta tcgggatttg ttatgaatct tcacacgata
     1501 ccttcccgaa agaaattaac cgggttggca gagacggtga ttgttttgat tttaatggcg
     1561 gcaggatttg ctgtcaggtt atatgatttg accgatccgc cgttggattt ctattctccg
     1621 agacagttac gctctgccat catcacccgc tctttttatt acgaaagtct gaacaacagt
     1681 gatccgacca ttgtcgaaca agccgataaa ctggccgatt tggaacgcta tgaaccgccg
     1741 gtgatggaat ggctggtatc caaaatcgat ttactgatcg ggaaagaagt tttttggacg
     1801 ggtcgcattt tcagtgcttt tttctgggcg ttaggcggac tgtttctgta tcggatctgc
     1861 cgtcgttttg cttccttttc agccggaata ttttcgttat tgatttatta ttttgtcccg
     1921 tttagtatta ttgcttcccg atcttttcag cccgatcctt ggatgtgctt ttgggtttta
     1981 ggcaccgctt ggatgttgtg caactggtct gagaaagggt catggtcttc ggcgtttttt
     2041 tccggattca tcgggggaat ggcgttgttg gtaaagattt atgcgggctt ttttgtcgcg
     2101 tttatgttat tggggatctt ctgcgcaagt gttaaccgcc aaaatcgaaa acaaaggata
     2161 tttcaaaccc ttttgatcgg cgcggtcatg ttgattccct cggcagttta ctatttgata
     2221 ttcaatcctt cccgttcggg agatttcttt tcattttgga ttgtttctct ttccggactg
     2281 attcggacct caaattttta tgcggattgg attgcgatgg tgaaagggct gatcggattg
     2341 gtcctgccgc tctttgggtt gtggggagtc cttttcatcc gaaatcgtca ggtaaaaggg
     2401 ttaatgatcg gttggtggct gggatacatt ttttacggat tatttgtccc ttatcagatc
     2461 acgactcatg aatactatag cctgattttg atcccattga cagcgatttc cgtatgtccg
     2521 gtttcggatg cgatttttga aaaaactcgg caaagcggaa agattgcctt cgtttgttcg
     2581 ctccttttga tcatggcggc agccggttac ggcgcctatt cggcttgcgg atatttacga
     2641 gggacgaatt atgttttgga accggcgagt tggagaaagg tcggggaagc gatcccgcaa
     2701 aatgagccgt ttatcgctct taccggagac tatgggatgc ggctgattta ttacggctgg
     2761 cggcaaccgt ccctgacttg gccggatgca aaagatgata cgctgtttca attggctggg
     2821 acacctcagg cggattttga tacttacttt gaatcccaga cgaaaggtat gaattatttt
     2881 gtagtgacag cgctgggaga gctggataat cagccggatc taaaaaaaag attggaattc
     2941 tttcctgttt atgccgctgg aaacggatat attatctatg atttgagaaa taaaccttga
     3001 gaaatattgt cgcacggaaa aaattatctt caatccatat cattatcttt atcctgatat
     3061 tattatgcgg gacttttttg cgattcgtcg attttgacga tccgccgctg gattttcatc
     3121 cggctcgcca attccattcc gcattaatcg ctcgcagcta ttttctgaaa gataacggga
     3181 atttacccgg gaaaagtgcc gattatcaac atgaggcgga aatgcgcggt tcacaggaac
     3241 ccagaatcga accgccgatt atggagagac tcgctgcgtg ggggtataaa attgtcggag
     3301 atgccgacct gcgggtaccg cgagcgatgt caatcgtttt ttggttaatc ggcgggatcg
     3361 gattatttcg attatgccgt cgatttctcg gaatcaccgg cagtctggtc ggtttcggtt
     3421 tcttcctttt attcccctat acgatttttg cgagcagatc atttcagccg gatcctttga
     3481 tggtcgcact gatgatatgg tcgctaaatg cgttggtcga atgggattca catccgacga
     3541 ttgggtcggc gatcctgacc ggggctgtgg cgggagctgc gattttggtc aagcaagtgt
     3601 gcgttttccc gttggcgtcg gcttttgcgt tttacttttt ttcgtcttcg gattggaaac
     3661 aaaaaataaa gagggttgat ttttggataa tcgtggctct ttctgtcgct ccggtgttga
     3721 tttataacta ctggggttac tttgtcaaag gttttttggt acaacaatat caaggtcgtt
     3781 tttctttgag cgattggttt tctccggcgt tttatgtccg ctggattcgg aagatggacg
     3841 aggtgtttgg aatcgttgtc gtaacagccg ggttagtggg ctgtcttgcc tgtgaaaatc
     3901 gaagagcacg tttattgtgg atcggttttt ttgccggata tattctttac ggtttggcgc
     3961 ttcctcacca tatcggaacc catgattatt atcaattgcc tttattcccg ctgatttctt
     4021 tcggaatcgg ccgatcggcc gaatcaatct ttgaggttgt cgggaggtta aaagaaggcc
     4081 ggacgcttgc ttatgtcgga attactgtct gcttgattct gttgggagct tggtggagtg
     4141 cggacagtgt catcagcttg aaccgaaggg attatcgagc ctggccggaa cgatggcaat
     4201 ctctcgcggc tgaattagat ccctacccgg gtcaaatcaa cacgatcgga attatggatg
     4261 attatggggc ggcaatgatc tattggggcc ttcggacacc ggtgatttgg gaacaaaata
     4321 tcgaaaagct ttcggaagag gaagccgaca ccaccattcg ccagacgatg ataaatcggg
     4381 aatacttaat tgtgacggac cttgacagct tttatgcgca gccgcggctt cagaattggt
     4441 tggttcgcaa tacgaagctc tatgcggaag aaccggacta tctgatttat gatctgagag
     4501 ggttaaatga gtgaaaaggc ggtttttccg ttcgtcagta ttgtcacacc ttcgtttaat
     4561 caggcggaat ttctcgaacg taccattcaa tccgttctga gtcaggatta tccgaacctt
     4621 gagtacatca ttatcgacgg aggatcgacc gacggaagtg tcgagattat tcgaaaatat
     4681 gagaaccgtt tggcttattg ggtttccgag aaggataaag gtcagacgaa tgccattaat
     4741 aaaggcttta atcgggcaaa aggagagatt tttgcctggc tgaattcgga cgacatctat
     4801 gagccgggag ccattcaagc cgctgttgaa gctctcatgt cggatccttc cttggggttt
     4861 gtatatggcg attgtaactt tatcgacagt catgaccgta ttatcgggaa attcgatgtg
     4921 cgacaaacgg atctaaaaaa attacagcgc ggatatgttc atattcctca acaagctttt
     4981 ttctttcgag gggagctctg gaaaaaagta ggtccgctgg atgaaagcat ctattttgcc
     5041 atggattatg atttgtggtt aagattggct cgaatcgcgc cttttcgata tataccggga
     5101 aaggtctggg ctgattttcg attgcatgaa gatgccaaga cgattaccgc ggatgcatct
     5161 tgttggccgg aaatgattca gattcgtcgc cgtgacggcg gatcgaaatt ttctgtcctt
     5221 ttcctgaaat atcaactgcg aaaattaata gctcctctga ttaattatcg acgtaaaaaa
     5281 atgtttcaat aaattttccg attaacacct gattttaaat aatttgttta taccaaaaat
     5341 attgacaaaa gtggggcggg tgatatataa taatagtccg ccttagaaaa agggttacgg
     5401 tggtcttgaa aaaaggaaaa aagatcgtaa tgacttttca aaaaagagcc ttgacgagat
     5461 caccgaaaca catataataa gacgttgcgc ctgggaaaca gaggggcgtg aagaaccttg
     5521 acaattgaga agcagcagaa atgaagaaac
//
LOCUS       DAOMIV010000075         5485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15059_length_5485_cov_5.238122, whole genome shotgun sequence.
ACCESSION   DAOMIV010000075 DAOMIV010000000
VERSION     DAOMIV010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15059_length_5485_cov_5.238122"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PKV59_02840"
     CDS             <1..784
                     /locus_tag="PKV59_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP45720.1"
                     /translation="HLIQPDSGEIWFDGTETAHLSENEFRPFRQHMQMIFQDPNSSLD
                     PNMRIFDLISEPLLIFTDMSDRERRERVRELLEVVGLSYNHAERYPHEFSGGQRQRIG
                     IARALSVNPKLIIADEPISSLDVSIQAQILNLLQKLQKQFNLTYFFISHDLSVVQLIS
                     DHVAVMYLGFIVEKAPKKELYQHPLHPYTQALLSAVPIPDPKIKKQRILLAGDLPSNI
                     HLPAGCPFNSRCFRCQDRCREEKPELREIEKDHFVACHFADL"
     gene            complement(835..2091)
                     /locus_tag="PKV59_02845"
     CDS             complement(835..2091)
                     /locus_tag="PKV59_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HQP45721.1"
                     /translation="MSSYPKNWFLDAKYGLFIHFGLYSLLGGEYKGKVTPFFSEWIMN
                     DLNIPLSEYEQLAAQFDPFEFDADKIAESALRWGTRYLCFTAKHHDGFALFGSSCSDY
                     NSVCRTPSRRDYVSELAEACRKKGIVFCLYYSQAQDWHHPDGYVAYHDNQNKNFRRYL
                     DEKCLPQLTELLSDYGPIGMMWFDTPMGMKAEECEEIYQLVKKLQPECLVSGRIGNGL
                     GDYFCTQDNRLPAFPLKRKWEIPGTLNRSFGYKKSDTNWDSAETVIRKLVKIVSRGGN
                     YLLNVGPDGSGKIPQESTKIMDEAGEFLSENGEAFYGAEAVPPYVYETPDIYFTHKPH
                     RLFLTVFHPGQFPELTVALPNIANIPQSAKILRGNRPIAFNYRKTLENDSYWEFFLPP
                     DAENQTVVVICVETQEADVRFEPLDG"
     gene            complement(2137..3108)
                     /locus_tag="PKV59_02850"
     CDS             complement(2137..3108)
                     /locus_tag="PKV59_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45722.1"
                     /translation="MPDKQPLVSVKNLKKYFNVGKNLTLKAVDDVTFDIYPGETLGLV
                     GESGCGKSTLGRTLIRLYHATDGEIWFDGMNLTKDLTSRESFQFTRRAQMIFQDPYAS
                     LNQRMKVMDIIAEGIDAHHLAADKKEREEMVVELLQTVGLQREHANRYPHEFSGGQRQ
                     RIGIARALAVNPDFVIADEPISALDVSIQAQIVNLLQDLQEKRGLTYLFIAHDLSMIK
                     HISDRVGVMYLGSLVELTTADELFANSLHPYTQALLSAIPLADPNLEKSRKRIVLQGS
                     LPSPINLGSGCRFASRCPQVTEVCKEVSPELIEVSPGHFVACHLTDR"
     gene            complement(3098..4105)
                     /locus_tag="PKV59_02855"
     CDS             complement(3098..4105)
                     /locus_tag="PKV59_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45723.1"
                     /translation="MGKILQVENLHVEIKTELGRIQAVRGVSFDLDKKETVAIVGESG
                     SGKTITVKSVMQLLPSTGSITEGSVRLEGKELTTLSEKEMLKIRGSDISMIFQDPMTS
                     LNPTMTVGNQIVEVLKNHRKDLSRDEMKRRAIEIIDLVGISNPETRFKQYPHQLSGGM
                     RQRIIIAIALACDPKVLIADEPTTALDVTIQAQILDLMKELQEKINTSIIIITHNLGV
                     VANIADRVYVMYGGKIVENGSVEDIFYHPQHPYTKGLLAAVPKLHEKTDKLFTILGTP
                     PDLTDPPKGCPFAARCSYTLKVCRQYPPAFTPFENHHESACWLHDPRAKRMINEVRNA
                     G"
     gene            complement(4114..5040)
                     /locus_tag="PKV59_02860"
     CDS             complement(4114..5040)
                     /locus_tag="PKV59_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45724.1"
                     /translation="MENYQDLPLDLFEKAEVNLDEKEHIAKPSLTAFQDGWIRLKKNK
                     AAVVSLFILILIFLFAVFGPIFSSYDYAKTDYHHTFQLPSREHIFGTDQFGRDQFARI
                     WYGTRISLLIAFAAACLDIFVGVTYGAVSALAGGKVDAVMQRIIEILVGIPSLIIIIL
                     LMMVMPAGIGTIIIAMSITGWVNMARLVRAQILKLKSQEFVLAARILGTNSRKIILKH
                     LIPNTMGVIVINTMFTIPSAIFTEAFLSFIGIGLSEPRASLGVLINNGYQVLHNFPHL
                     LIYPAIVIVLIMVCFSILGDGLRDALDPRMRK"
     gene            complement(5044..>5485)
                     /locus_tag="PKV59_02865"
     CDS             complement(5044..>5485)
                     /locus_tag="PKV59_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP45725.1"
                     /translation="FWGTWAHSVLPSIALSFGATAMFARFIRTEMLDVLEQDYIITAR
                     AKGLNQFQVLMAHAVRNSIIPVVTILGPIVVNLLTGSLAVESIFSVPGIGSLFVESIK
                     SNDYATIMGLTIFYSAFYILVVMIVDILYSVIDPRIRIQGNKDE"
BASE COUNT         1536 a         1337 c         1143 g         1469 t
ORIGIN      
        1 ccatctgatt caacccgatt ccggtgaaat ttggtttgac ggaacggaaa cggcccatct
       61 cagtgaaaat gaatttcggc cctttcgcca acatatgcag atgatcttcc aagatccgaa
      121 ttcttctctg gatcccaaca tgcggatctt tgatttaata tccgaaccgt tactgatttt
      181 taccgatatg tcggatcggg aaagacggga acgcgtccgc gagttattgg aagtggtcgg
      241 cttaagttac aaccatgccg aaagatatcc gcatgaattt tccggcggac aaagacaacg
      301 gatcggcatc gcccgtgcct tatcggttaa ccctaagctg atcattgccg acgaaccgat
      361 ttcgtcgtta gacgtatcca ttcaagctca aatattaaac ctgttacaga aattacaaaa
      421 acagttcaac ttaacctatt tttttatttc ccatgacttg agcgtcgttc aattaatctc
      481 ggatcatgtt gcggtaatgt atttggggtt catcgttgaa aaagcaccta aaaaagaact
      541 ttatcaacat ccgcttcacc cttatacaca agcattacta tccgctgtcc cgatccccga
      601 tcccaaaatt aagaaacaac gtattttatt ggcgggggat ctccccagca atattcactt
      661 gcctgcggga tgccctttca attcccgttg tttccgctgt caggatcgat gccgagaaga
      721 gaagccggaa ttacgtgaaa tcgaaaaaga tcattttgtg gcttgtcatt ttgccgatct
      781 gtgaccattt aagcaaataa tcctgtcccg ggatgaatgc ttttcgaaat ttgattagcc
      841 atcaagcggt tcaaaccgca catccgcctc ttgcgtctca acacaaatga cgacaaccgt
      901 ttggttttcc gcatccggcg gaaggaaaaa ttcccagtag ctgtcattct ccaacgtttt
      961 tcgataattg aacgctatcg gtcgatttcc gcgcaaaatc ttggcgcttt gaggaatatt
     1021 ggcaatattc ggtaaggcaa cggttaattc cggaaattgt cccggatgaa agacagttaa
     1081 aaacagtcga tgaggtttat gagtgaaata gatatccggg gtttcgtaaa cataaggagg
     1141 gaccgcctcc gccccgtaaa atgcttcacc gttttcggac aagaattcac cggcttcatc
     1201 cattattttg gtgctctctt gcggaatttt gccgctgccg tcggggccga cattcagcag
     1261 ataattgccg ccgcgactga caatcttcac cagtttgcga atcactgttt ccgcggaatc
     1321 ccagtttgta tccgatttct tatatccgaa tgaacgattc aacgtcccgg gaatttccca
     1381 ttttcgcttc aaagggaaag cgggcaagcg attatcttga gtacagaaat aatcacccaa
     1441 tccgttcccg atcctcccgc tgaccaaaca ttcgggttgt agttttttaa ccagttgata
     1501 aatctcttcg cactcttccg ctttcatccc catcggtgta tcaaaccaca tcattccgat
     1561 cggaccgtaa tcggacagca actcggttaa ttgcggaagg cacttttcat caagatatcg
     1621 gcgaaaattt ttattttgat tgtcatgata agcgacataa ccatccggat gatgccaatc
     1681 ctgagcttgt gaataatata aacaaaagac gattcctttt ttccggcaag cctctgccaa
     1741 ttcggagaca tagtcccgtc gggatggtgt ccggcagaca ctgttatagt ccgaacagga
     1801 tgaaccaaac aaagcaaacc cgtcatgatg tttcgcggta aaacagagat aacgggttcc
     1861 ccaacgaagg gcggattccg cgattttgtc ggcatcaaat tcaaacggat caaattgagc
     1921 ggcgagctgc tcatattcag acagcggaat attcaagtca ttcatgatcc attcggaaaa
     1981 gaacggcgta acttttcctt tgtattcgcc gcccaaaagg gaatacaagc caaaatggat
     2041 aaataatcca tatttcgcat caagaaacca gtttttcgga tatgaactca taaaggaatc
     2101 ctttcgatcg gatttttcgc agttaaaaat cggttttcag cgatccgtaa gatgacaggc
     2161 gacaaaatgc cccggtgaca cctcaattaa ttccggagaa acctctttgc atacttccgt
     2221 cacctgcgga catcgggaag caaatcggca tccgcttccc aaattaatgg ggctgggcag
     2281 acttccctgc agcacaatcc gtttccggct tttttccaaa ttcggatcag ccaaaggaat
     2341 cgcagacaac aacgcttgtg tatacggatg caaagaattt gcaaataatt catcagcggt
     2401 tgttaattcc accagactgc ctaaatacat cacaccgacc cgatcgctga tatgtttaat
     2461 catagataaa tcgtgggcga taaacaagta agtcaatccg cgcttttcct gtaaatcctg
     2521 aaggaggttg acaatttgag cttgaatcga gacatccaat gcggaaatcg gttcatcggc
     2581 aatgacaaaa tccgggttga ctgcaagcgc tcgggcgatg ccgatccgtt gtcgctgtcc
     2641 accggaaaat tcatgcggat aacggtttgc atgttccctt tgcagtccga ccgtttgaag
     2701 cagctccact accatttcct ctcgctcttt cttatcggca gctagatgat gagcatcaat
     2761 cccctcagca ataatatcca tcactttcat gcgttgattc aaagaggcat acggatcttg
     2821 aaagatcatt tgcgcccggc gtgtaaattg aaaactttcc cgggaagtta aatctttggt
     2881 caaattcata ccgtcaaacc aaatttctcc gtcagtcgca tgataaagtc gaatcaatgt
     2941 tcgtccgaga gtcgattttc cgcagccgct ttctccgacc aacccgagtg tttcacccgg
     3001 ataaatatcg aatgtcacat cgtcaaccgc cttcaaggtt aggttctttc ctacattaaa
     3061 atatttcttt aaatttttaa ccgaaaccaa aggctgttta tccggcattt cgcacctcgt
     3121 taatcattct ttttgcccgc ggatcgtgca gccagcaagc ggattcgtga tggttctcaa
     3181 aaggagtaaa ggccggtgga tattgccggc aaacctttaa agtatacgaa catcgagcgg
     3241 caaaaggaca gcctttcgga ggatctgtca gatcaggcgg ggttcccaaa atcgtaaata
     3301 acttgtccgt tttttcatga agtttcggta cggcagccaa caatcctttg gtatacggat
     3361 gttgcggatg atagaaaata tcttccacgc tgccgttttc cacaatcttg ccgccgtaca
     3421 tcacataaac gcgatcggcg atattggcaa ccacgcccaa attatgagta ataataatga
     3481 tagaagtatt aatcttttcc tgcagttctt tcattaaatc aaggatttgt gcctgaatgg
     3541 ttacatccaa agcggtcgtc ggctcatcgg caattaatac cttaggatca caagccaaag
     3601 caattgcgat aatgatccgt tgccgcatac ctccggataa ctgatgggga tattgtttga
     3661 aacgggtctc gggattggaa atccccacca aatcaatgat ctcaatagcg cgtcgtttca
     3721 tctcatcccg acttaaatct tttcgatggt ttttcaatac ttcgacgatt tggttcccga
     3781 ccgtcatggt cggattcaaa ctggtcatcg gatcctgaaa gatcatggat atatccgagc
     3841 cgcgtatttt cagcatctct ttttcgctga gcgttgtaag ctctttcccc tccaatcgaa
     3901 ccgatccttc ggtaatcgaa ccggttgaag gcagcagttg cataacgctt tttaccgtaa
     3961 tcgtcttacc gctgccggat tcaccgacga tggcaacggt ttccttttta tccaggtcaa
     4021 aactgacacc gcgaaccgct tgaattctgc ccaattccgt tttgatttcg acatggagat
     4081 tctccacctg taatattttt cccattcagc ctcctatttc cgcatccgcg gatcaagcgc
     4141 atcgcggagg ccgtctccca aaatactgaa acagaccata atcaaaacga tgacgatcgc
     4201 cggatagatc aaaagatggg gaaaattatg aaggacctga taaccgttat taatcagaac
     4261 ccccaaagat gcgcgaggct cactgagccc gatcccgata aagctgagaa atgcttcggt
     4321 aaaaattgcg ctcgggatcg taaacatcgt gttgatgaca atcacaccca tcgtattcgg
     4381 aatcagatgt ttcagaatga tttttcgtga attcgtaccc aaaattctcg ctgccaaaac
     4441 gaattcctga ctttttaatt tgaggatctg agcccgtacc aatcgagcca tgttgaccca
     4501 gccggtgatg gacattgcga taataatggt tccgataccg gctggcataa ccatcattaa
     4561 cagaatgatg atgatcagac tagggattcc caccaaaatt tcaatgatcc gctgcatcac
     4621 cgcatcaact ttccctcccg ccaatgcgga aaccgctccg taggttactc cgacaaatat
     4681 atctaaacag gcggcggcaa aggcgattaa taatgagatt cttgtaccgt accagatccg
     4741 ggcaaattga tcgcgtccga attgatccgt tccgaaaatg tgttcccgtg acggaagctg
     4801 aaatgtgtga tgataatccg ttttagcgta atcgtagctg ctgaaaatcg gtccgaaaac
     4861 cgcaaaaagg aaaataagaa tcaaaataaa aaggctcaca acggcggctt tattcttttt
     4921 taaacgaatc cagccatcct gaaaagccgt caaactgggc tttgcgatat gttctttttc
     4981 atccagattg acttccgctt tttcgaataa atcaagtggt aaatcctgat aattttccat
     5041 ggcttattcg tccttatttc cctgaatacg tattctggga tcgatgacgg agtagagaat
     5101 atccacgatc ataaccacca aaatataaaa tgcactgtaa aaaatcgtta atcccataat
     5161 tgttgcataa tcattgcttt taatactttc gacgaacaga ctgccgatgc cggggaccga
     5221 aaaaatgctc tccaccgcca aggatccggt caaaagattg accacgatcg ggcccagaat
     5281 cgttactacc ggaataatcg aatttcggac tgcatgggcc atcaacacct gaaattgatt
     5341 caatcccttg gcgcgggcgg tgataatgta atcctgttct aaaacatcca acatctcggt
     5401 tcggataaaa cgggcgaaca tagccgttgc gccaaaggag agcgcaatcg atggcaaaac
     5461 ggaatgtgcc catgtccccc aaaag
//
LOCUS       DAOMIV010000076         5485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15061_length_5485_cov_4.991713, whole genome shotgun sequence.
ACCESSION   DAOMIV010000076 DAOMIV010000000
VERSION     DAOMIV010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15061_length_5485_cov_4.991713"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PKV59_02870"
     CDS             complement(<1..441)
                     /locus_tag="PKV59_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HQP45726.1"
                     /translation="MDIKAILLDFDGTALQRDQVFISCRNMYAIRQAIAKGIEIIPCT
                     GRCEDMFPPQIEAEKRIRYWITASGTRIVDRNEKKVLYQCVFSPEESAQLCEIFENQD
                     IYTEISAEGHIYLEKKINDHLERYPVPSHHVWFVESGRQIPVEQP"
     gene            complement(665..1129)
                     /gene="bfr"
                     /locus_tag="PKV59_02875"
     CDS             complement(665..1129)
                     /gene="bfr"
                     /locus_tag="PKV59_02875"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HQP45727.1"
                     /translation="MKGSEKVIQALNANLKDEMTAIDQYILHAEMCEDWGYSKLHDSI
                     EKRAITEMKHAEALIARILFLEGIPIISELGKMSIGKDVEEMMAFDHKAEHDAIQSYN
                     NAIKVCVEEKDNGTKELFEATLKDEEDHIDWIESQIDQISQMGIQNYLAKQV"
     gene            complement(1252..1500)
                     /locus_tag="PKV59_02880"
     CDS             complement(1252..1500)
                     /locus_tag="PKV59_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45728.1"
                     /translation="MDYSMISKLEKAKRYAKERDRFKIHSLEATVEGSNGPHVVKYKD
                     GKWECDCSFFQGRGRCSHTMSLEIILEGMVDIAYEDQQ"
     gene            complement(1638..2147)
                     /locus_tag="PKV59_02885"
     CDS             complement(1638..2147)
                     /locus_tag="PKV59_02885"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQP45729.1"
                     /translation="METIFAPWRGEYINGIGNHAKNPDNPQECVFCSAIKATDDFERY
                     IIYRGINAFVILNLYPYNNGHMLIIPYNHVSDLSQLNTETHHEMMDLIVKAQKVLQDV
                     YHPHGINIGANIGTAAGAGIDQHLHFHILPRWTADVNFMTTIGQTRVIPEKLDETWKK
                     IRASWQKID"
     gene            complement(2376..3698)
                     /locus_tag="PKV59_02890"
     CDS             complement(2376..3698)
                     /locus_tag="PKV59_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45730.1"
                     /translation="MTKSTARKITSFLFAYKVPVLFILLSIGGILVSGQSLNFILMEL
                     ASRFGRDTFLVLALIIPVVSGMGLNFSIVVGAMAAQIAIFWVMHLLQTVIPTCPPVLA
                     FLLCVLISTPIASLFGYLAGKLFNMMKGAEMIGGLILGYFSDGLYQLLFLFIFGGLIP
                     IANKTLMIATGIGVKNTIDLSSTKKVNGLKYAIDNIWKVPLTEFLQLVFAGVVLYAIG
                     AFLYGKIKNRKVLSGRFWILFGISAILFGVSFIPAFIQFSMIISIPVVTYMLIAVFCL
                     FIQWFLKTRLGQNMRAVGQNKVVANAAGINVDKVRIISIIISTVMAAFGQLIYLQNIG
                     VFTTYGAHKNVALYSIAALLVGGASVYKATIGQAILGIILFHILFIVSPLAGQTLMND
                     AMIGEYFRVFICYGVIAMSLAMHAWTMKNTKGKKVTEPIALESEVKDN"
     gene            complement(3698..4750)
                     /locus_tag="PKV59_02895"
     CDS             complement(3698..4750)
                     /locus_tag="PKV59_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45731.1"
                     /translation="MADRLKSIYHSFGLPRIIIILFFLALLIAGGLLEIPVPSLISDV
                     LVRFGMNGILVLAMVPGIQAGIGLNFGLSLGIVAGLLGGVISIESGFSGFAGLAVAIG
                     IGILLSIVIGIGYGLLLNSIKGSEMTVSTYVGFSAIALMNIFWLILPFHNPDMVWAIG
                     KGLRATIDLSSRYKHILNEFMTFNIPTSAGDIVVPTGLILFFFISCFIVWLFLRSKSG
                     IAMSAAGGNPKFALAAGIDENKTRILGTTISTVLSAVGIIVYAQSYGFYQIYNAPLMM
                     SFAAAAGVLIGGASISRAKISHVIIGTFLFQGLLVLGLPVANKLLPEGNLSEVMRIIV
                     SNGIILYALTKSQGDK"
     gene            complement(4743..>5485)
                     /locus_tag="PKV59_02900"
     CDS             complement(4743..>5485)
                     /locus_tag="PKV59_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP45732.1"
                     /translation="MPGEEVKGVDLKVREGEIFGIGGLAGQGKVGISNGIMGMFPAAG
                     EVIIFGKNVRYSVIGETLKHHTAFVSEDRRGVGLLLDDSIALNIIFSAMQTQNRFLRK
                     FGPLSQINSFEVQSFAKEMIHKLDIRCTGPDQAVGSLSGGNQQKVCLARALTLNPQIL
                     FVSEPTRGIDIGAKKLVLDELLKLNREKGVTIVMVSSELVELRSICDRIAIVSDGKVF
                     KILNPDASDIEFGLAMSGTLAEEELANG"
BASE COUNT         1639 a         1250 c         1034 g         1562 t
ORIGIN      
        1 gggctgctca acggggatct gtcttcccga ttcgacgaac catacatgat gagaaggaac
       61 gggataacgt tccaaatgat catttatttt tttctcaaga taaatatgac cttccgccga
      121 aatttcggta taaatgtctt gattttcaaa tatctcgcat aattgggcag attcttcagg
      181 tgaaaaaacg cactgataca atactttctt ttcgtttctg tcaacgatac gcgtgcctga
      241 ggctgtgatc caatacctga ttcttttttc ggcttcgatc tgagggggaa acatatcttc
      301 acatctgcct gtacagggga taatttcaat cccttttgca atcgcttgtc tgatcgcata
      361 catgtttcga caagatataa atacttgatc cctttgcaat gctgtaccgt cgaaatcaag
      421 taatatggct ttgatatcca aaaatactcc tttaggcaag ataatcaatt taaaaatgtt
      481 tttaacgata aatagattta atcgttattt aataagaata tctgacataa aacaaactgc
      541 gattttcaaa atcctataca aattgtaaaa caagtctttg atcgagtcaa agacttgttt
      601 tttattataa ttccctgcat tacggatttt tcgaagggaa acagataacc tccgaatcgc
      661 aactttacac ttgttttgcc aagtaattct gaattcccat ttgactgatt tgatcgattt
      721 gagattcaat ccaatcaata tgatcttctt catcttttag tgtggcttca aacaactctt
      781 ttgtaccgtt gtctttttct tcgacacaaa ccttaatggc attgttgtaa gattgaatgg
      841 catcgtgttc ggctttatgg tcaaaagcca tcatttcctc aacatctttc ccgattgaca
      901 ttttgccgag ttcactgata atcggaattc cctccaagaa aaggattctt gcaatgagtg
      961 cttccgcgtg tttcatttcg gtgattgcac gtttttcaat actgtcatgg agttttgaat
     1021 atccccaatc ttcacacatt tcggcatgta atatatattg atcgatcgca gtcatttcat
     1081 cttttaaatt cgcattcaaa gcttgaatta ctttttcgct acctttcata atttctttcc
     1141 tccttaatat tttctggttt gctattgttg tagcaaaaac aatttaggaa tatttacatg
     1201 tcaaaaaagc ccgtatggat tcaggcttat aacgcctata ttcaccaatt tttattgctg
     1261 atcctcgtaa gcgatatcaa ccatgccttc gagaataatt tcaagggaca tcgtatggct
     1321 gcaacgaccc ctcccctgaa aaaaactgca atcacattcc catttcccat ccttatattt
     1381 tacgacatga ggtccgtttg atccctcaac ggttgcttcg agagaatgaa tcttgaaacg
     1441 atcacgctct ttggcatatc tttttgcttt ttccagttta ctgatcatcg aataatccat
     1501 ttcttattct tcccccagtc tattgcaaga tattttgaaa aaacgctttg attctatact
     1561 atacctgacc taaagaagat tcgtcaattg tttttcatct tattttaata tgacagaatc
     1621 ataaaataac catttcttca atcgatcttt tgccaagatg cacgaatttt tttccacgtt
     1681 tcatccaatt tttccggaat gacccgagtc tgaccgattg tcgtcataaa attcacatcg
     1741 gcagtccaac gaggcaaaat atgaaaatga agatgttggt cgatgccggc tccggcagcc
     1801 gttccgatat tcgccccgat attaattcca tgcggatgat atacatcctg aaggactttc
     1861 tgcgctttaa cgatcagatc catcatttca tggtgagttt cggtattgag ttgcgataaa
     1921 tcactaacat ggttataagg aataatcaac atatgtccgt tgttgtacgg ataaagattc
     1981 aaaatcacaa aggcattgat tcccctgtaa atgatgtatc gttcaaaatc gtcggttgct
     2041 tttatggctg agcaaaaaac acattcttgc gggttgtccg gattctttgc atgatttccg
     2101 ataccgttga tatattcgcc ccgccacgga gcaaagattg tttccatttt atttttccct
     2161 ccggaatttt gaaaactttc tccgattcaa acatcaggga gtaccttaat tataaaccgt
     2221 aaccaagtga aaaaaatttc ggtcaatcag gctcgatttg aataggagac cgaataaaaa
     2281 gatcggtttt tcttcttgca aaaaggcttt ggcaatccgt tgagatcacc aaagcctttt
     2341 ccaagcacgt ttgaatatca aaagcggtgc ttatttcagt tatcctttac ttcactttcc
     2401 aatgcgatcg gttcggtcac ctttttccct ttcgtatttt tcatcgtcca agcatgcatt
     2461 gccagggaca tggctatgac accatagcag ataaatacac ggaaatattc gccgatcatt
     2521 gcatcattca ttaatgtctg tccggctaac ggagatacga taaacagaat atggaacagg
     2581 ataatgccga gaattgcctg accgatggtt gctttgtaaa ccgaagcacc gccgactaaa
     2641 agcgcggcaa tggaatagag ggccacattt ttgtgtgctc cgtaagtagt gaaaaccccg
     2701 atattctgta aatagatcaa ttgaccgaac gccgccatta ccgtggatat aatgatcgag
     2761 ataatccgta ctttatcaac attgattccg gcagcattgg caacaacttt gttctgaccg
     2821 acagcccgca tattttgccc caatctggtt ttcaaaaacc attgaataaa taggcaaaaa
     2881 accgctatta acatgtaagt gacgaccggg atactgataa tcatcgaaaa ttgaatgaaa
     2941 gcgggaataa aagaaacgcc gaataatatc gcactgattc cgaaaaggat ccaaaaacga
     3001 ccgctgagca cttttcgatt ttttattttt ccatagagga aagcaccgat tgcataaagg
     3061 acaactccgg caaataccag ctgaaggaat tcggtcaaag ggactttcca aatattatcg
     3121 atagcatatt tcaatccgtt cacctttttg gtactggata agtcaatggt attttttacc
     3181 ccgataccgg tggcaatcat caatgtttta ttggcaatgg ggattaatcc gccaaaaata
     3241 aacagaaata aaagttgata gagaccgtcg gaaaaatatc ccaaaatgag accgccgatc
     3301 atttcagccc ctttcatcat attgaataat tttccggcaa gatatccgaa cagggaagcg
     3361 atgggcgtac tgatcagcac acacagcaaa aatgcgagca ccggcgggca agtcggaata
     3421 accgtctgca ataagtgcat tacccaaaaa atggcgattt gggcagccat cgcgccgacg
     3481 acaatactga agttgagtcc cattccgctg acgaccggaa taattaaagc cagtaccaaa
     3541 aaagtatccc gtccgaaacg ggaagccaat tccatcaata tgaaattcaa agattgtccg
     3601 gagacgagta ttcctccgat actcagaaga atgaacagta ccggtacttt atatgcgaac
     3661 aggaaggaag tgatttttcg cgcagttgat tttgtcatta tttatctccc tgtgatttgg
     3721 ttaaagcata caaaatgatg ccgtttgaaa cgatgattct catcacttcg gaaaggttcc
     3781 cttccggcaa taatttattc gccaccggca acccgagtac cagcaaccct tggaaaagaa
     3841 acgtcccgat aataacatgc gaaatcttgg cgcgactgat gcttgcccca ccgatcagaa
     3901 caccggctgc agccgcaaaa ctcatcatca gcggtgcgtt atagatttga taaaaaccgt
     3961 aactttgtgc atatacaatt atgccgaccg ctgataatac ggtagagata gtcgttccta
     4021 aaatacgcgt cttattctca tcgattcctg cggctaatgc aaatttcgga tttccgccag
     4081 ccgcagacat ggcaataccg gatttggatc gcaaaaacaa ccatacaatg aagcaactga
     4141 taaaaaagaa taaaatcagt ccggtcggaa ccacaatatc gcccgcggaa gttggaatat
     4201 tgaaagtcat aaactcattt aaaatatgtt tataccggct gctgagatcg attgtggcac
     4261 gaagcccctt tccgatcgcc cagaccatat ccggattgtg aaacggcaga atcagccaaa
     4321 aaatgttcat caacgcaatc gccgaaaaac cgacgtatgt cgagacggtc atttccgatc
     4381 ctttaatgga attgagcaac agaccatatc cgattccgat gacgatggaa agcagaatgc
     4441 cgattccgat cgccacagct aaaccggcaa aacccgaaaa accggactcg atactgataa
     4501 cgccgcctaa caggcctgct acgatgccga gcgaaagacc gaagtttaat ccgattccgg
     4561 cttgaattcc cggaaccatt gcaagaacca aaattccgtt cattccgaag cggaccagaa
     4621 catcgctgat taatgacgga acgggaattt caagcaaacc tccggcaatc aataaagcca
     4681 agaaaaacag aataataatg attcgaggta agccgaaaga atgataaatt gatttgagac
     4741 gatcagccat ttgccaattc ctcctccgcc agcgttccgg acattgccag cccgaattca
     4801 atatcgcttg catccggatt gagaatcttg aatactttcc cgtccgaaac gattgctatt
     4861 cgatcgcaaa tcgatcgcaa ttcaaccaat tcactggaaa ccataacgat ggtaacgcct
     4921 ttctctctgt ttagttttaa taactcgtcc aacaccaatt tttttgcgcc gatgtcaatc
     4981 ccgcgcgtcg gctcggatac aaaaaggatc tgagggttca gggtcaaagc tctcgccaag
     5041 cagactttct gttgatttcc cccggataaa gaacccaccg cctgatcggg accggtacag
     5101 cgaatatcaa gcttatgaat catttctttc gcaaatgact gcacctcgaa gctgttaatc
     5161 tgagaaagcg gtccgaattt tctgaggaaa cggttttggg tttgcatcgc ggagaaaatg
     5221 atatttaatg cgatggaatc atccaaaagc aacccgactc cccggcgatc ttcggataca
     5281 aaagccgtat gatgtttcag agtttctccg attacagaat atctcacatt ttttccgaag
     5341 ataatcactt cgccggcagc gggaaacatc cccataatgc cgttggatat acccactttc
     5401 ccctgtccgg ccaatccgcc gattccgaaa atttcgcctt cacgaacttt caggtcgacg
     5461 cctttcactt cttcaccggg catat
//
LOCUS       DAOMIV010000077         5485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15064_length_5485_cov_3.897238, whole genome shotgun sequence.
ACCESSION   DAOMIV010000077 DAOMIV010000000
VERSION     DAOMIV010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15064_length_5485_cov_3.897238"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="prfA"
                     /locus_tag="PKV59_02905"
     CDS             <1..817
                     /gene="prfA"
                     /locus_tag="PKV59_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQP45733.1"
                     /translation="KKENLENEIRSMLLPKDPRDSRNVIMEIRAGTGGDEAAIFAADL
                     FRMYSHYAEARHWHIEVMSANEIGLGGYKEIVFMVKGKGAYSRLKYESGIHRVQRVPI
                     TESQGRIHTSAVSVAVLAEVEDVDIQIPESDIRIEVYKSAGAGGQNVQKNSTAVRLIH
                     LPTGMVITCQDERSQLQNKTRAMSILRARLYDMEQQKRQEQLDATRRSMVGTGDRSEK
                     IRTYNYPQSRVTDHRINVSSFNLAGVMDGDLDVFIDELIMREQTEKLSVSEGA"
     gene            814..1677
                     /gene="prmC"
                     /locus_tag="PKV59_02910"
     CDS             814..1677
                     /gene="prmC"
                     /locus_tag="PKV59_02910"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQP45734.1"
                     /translation="MNGSDSVGEWLDWARRELQTSGYSDTPSIEAQWIFEKVTGAHRP
                     ELLSHPEKPLSDTEITKIRDMVEKVKEGVPLPYVLGEWSFFGNTFQITPDVLIPRPET
                     EILVEQAIEWAKNRLMPIRFADVGTGCGCIAVSLLNYFKTARFTGAAIDISDAALRVA
                     RRNIDHYRLNEVLFPIRGNLTECLSSPLDLVCANLPYIPSERCRSLDVAQNEPLLALD
                     GGEDGFDLYRMLLRDLTGKMAKESLILCEIDFSQKREALSLSRQLFPDASVTIKEDYA
                     GLSRLLMIRKS"
     gene            1680..2315
                     /locus_tag="PKV59_02915"
     CDS             1680..2315
                     /locus_tag="PKV59_02915"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQP45735.1"
                     /translation="MKKIYDITSPQIQPVAIQQAIDVLNSGGVVIFPTDTVYGLAASA
                     FNEGAIQRLYRMKERDPQKSIAVLLGNISQIRLIAADFPESARKLAERYWPGGLTLLV
                     SKLDTLPPALSANEKIGIRIPDYPVIQDLIIHTGPLATTSANLSGEPPAKKLSDLPDS
                     LLRQADLILDGGPVKGGIASTVVDCTVRPLLVLREGAISKEEVAAFDSETE"
     gene            2312..3469
                     /locus_tag="PKV59_02920"
     CDS             2312..3469
                     /locus_tag="PKV59_02920"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HQP45736.1"
                     /translation="MNPKQSGIPMRLRIWIVLCMLIISGCSKTDSHRPLSGGITDLFT
                     LKTVTMPALATSRPTKTATPTPTSEKSLTATITPTETSTPLPIVTPTSFFVDISTYPF
                     SASDKLEVIEDITIPDGTMLKPGQMFIKSWRIKNVGNKIWNNNYRLALAYSNPFNTPQ
                     MTHAIFIQPTELIDFAISTWNPRQYNVGVNDTVDLVIPLQAPEQAGDYMVEFFLINSD
                     DEIVPPKIWIRFSVELLPQFATQTAVAIAELTETATPPSERVRVPTPTPQPFDWTGTW
                     LIRNPYSDTGMIPVQAWLTQSGDTVTGFFYDTDEEPVLIEGTVDRENQGFIGKFAQPW
                     QNRAVPVKWRMLINRNQFYAVTDSGETEFGTICGGRNGMAFPEHCSMPPGI"
     gene            3482..4480
                     /locus_tag="PKV59_02925"
     CDS             3482..4480
                     /locus_tag="PKV59_02925"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HQP45737.1"
                     /translation="MIWIPNENTDPAFNLAAEEVILSSLNIKEETLFFYINEPSIIIG
                     RHQNTREEINAEFVGEQRIRIVRRLSGGGAVYHDFGNLCYSFIYPFPDRLTPDFALFT
                     APIVQALQNLGVPAELSGRNDILVAGAKISGNAYYHNRFGSVCHGTLLFDSDMGMLER
                     ALRPKPEKIERKGVRSVRSRVTNLKDYLPDIKDVKELRQAILYSISRTQPDFKEFRLA
                     ENDILKIESLAESRYRSDRWNFGKSPKYNYRVCRQFSFGEVDARLWVEDNRIREIRFF
                     GDFFSSEDPDSFSEKLIGIPFESQAIAQVAQQSDMEKLFPGISAVEFADFLTGKRS"
     gene            complement(4544..5329)
                     /locus_tag="PKV59_02930"
     CDS             complement(4544..5329)
                     /locus_tag="PKV59_02930"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQP45738.1"
                     /translation="MKSRFHLCENAEKLRNFCLSFYAIPLHFIISKCCLNSKEKNSAC
                     EEDPYKKDREQLFDFAAKYMNLKNPDILRACKTVPRHKFVPSREADQAYDDCALPIGC
                     GQTISQPSLVAQMIELSELKPGDKVLEVGTGSGYQTALLAELGYGDVFSVEIIPQLYE
                     RAKKILNELGYTNVKLKLGDGYYGWPEYAPYDAIVVTAAAEKVPPPLIEQLDEGGILL
                     IPIGPRNRSQTLWKLVKENGKLTRKDVGNVIFVPFVGGGIRND"
BASE COUNT         1457 a         1293 c         1268 g         1467 t
ORIGIN      
        1 caaaaaagaa aatttggaga atgagatccg ctcgatgctt cttccgaaag acccgcggga
       61 ttcacggaac gtgatcatgg aaatccgtgc cggtacgggc ggagatgaag ccgcgatttt
      121 tgccgccgat ttattccgca tgtattcaca ctacgcggaa gcccgccatt ggcatatcga
      181 agtgatgtcc gcaaacgaaa tcggcttggg cggatataaa gaaatcgtct ttatggtcaa
      241 gggaaagggc gcttattcac ggctgaaata cgaatcgggc attcatcgcg tccaacgggt
      301 gccgatcacc gaatcacagg gaagaattca tacctcagcc gtctccgttg ccgtattagc
      361 cgaggtcgag gatgttgata ttcaaattcc cgaatcggat attcggatcg aagtttacaa
      421 atccgccgga gccggcgggc aaaatgttca gaaaaactct accgcggtcc ggttaattca
      481 tcttccgacc ggtatggtca taacctgtca ggatgaacgt tctcagttac agaataagac
      541 ccgggcgatg agtattttac gtgcccgtct ttatgatatg gagcaacaga agcgtcagga
      601 gcaattggat gccacacgtc gttccatggt gggcacggga gatcgatcgg aaaagattcg
      661 gacttacaat taccctcaat cacgggtaac tgatcatcgg attaatgtct cttccttcaa
      721 tcttgccgga gtgatggacg gcgatttgga cgtttttatt gatgaattaa tcatgcggga
      781 acaaacagaa aaattatcgg tttcagaagg ggcatgaacg gttccgattc ggtcggtgaa
      841 tggttagatt gggcacgccg ggaattacaa acgagcggtt acagcgatac gccttccatt
      901 gaagcccaat ggatttttga aaaagtgacc ggagcgcatc gtccggaatt gctttctcat
      961 ccggagaagc cgctttccga taccgaaata acgaaaatcc gagacatggt agaaaaagtc
     1021 aaagaaggcg ttccgctacc gtacgtttta ggagaatgga gctttttcgg taatactttt
     1081 caaatcacgc cggacgtatt gattccccga ccggaaacgg aaatattggt cgaacaggct
     1141 atcgaatggg caaaaaatcg cttgatgccg atccgatttg ccgacgtggg aaccggttgc
     1201 ggctgtatcg ctgtttcttt gctgaattat tttaagacag ctcgctttac cggtgcggca
     1261 atcgatatct ccgatgcggc attaagggtc gcccgccgga atatcgatca ttatcggttg
     1321 aatgaggttc ttttcccgat ccgcggaaat cttacggaat gtctctcttc tccattggat
     1381 ttagtctgtg ccaatttgcc ctatattccc tcggaacggt gccggtcatt ggacgtagca
     1441 caaaatgaac cgctgctcgc actggatggc ggagaagatg gttttgatct ttatcgcatg
     1501 ttattacgag acttgacggg aaaaatggca aaggaatcat tgattctgtg cgaaattgat
     1561 ttttctcaaa aacgagaagc tttgtccctt tcacgccagc tgttccccga tgcatccgtt
     1621 acaataaaag aagattacgc cggcctttcc cgtttattaa tgattcggaa atcatgacta
     1681 tgaaaaaaat atacgatatt acttctcctc agatacaacc cgttgccatt caacaggcta
     1741 tcgatgttct gaattcggga ggtgtggtta tttttccgac cgatacggtt tatggattgg
     1801 ctgcttccgc ctttaatgaa ggagcaatcc agcgtcttta tcggatgaaa gaacgtgatc
     1861 cccaaaaatc aatcgcggta ttgctcggaa atatctccca aatccgatta attgccgcgg
     1921 attttccgga aagtgcaaga aagcttgccg aacgttattg gccgggcgga ttgacactgc
     1981 tcgtttccaa actggatacg ttgccgccgg cactttccgc caatgaaaaa atcgggatac
     2041 ggattccgga ttatccggta atccaggatc tgattatcca caccggccct ttggcgacaa
     2101 catccgccaa cctttccggt gaacctcccg caaaaaaatt gtccgattta ccggactctc
     2161 tgctccggca agcggatctg atcttggatg gcggaccggt aaaaggcgga atcgcctcaa
     2221 cagttgtcga ttgtacggtt cgtccgcttc tggttctgcg ggaaggtgcg atttcaaagg
     2281 aagaagtcgc cgcgttcgat tcggagacgg aatgaatcct aagcaatccg gaattccgat
     2341 gcgattgaga atctggattg ttttgtgcat gttgatcatt tccggttgtt caaagaccga
     2401 ttctcatcgg ccattgagcg gcggaattac cgatttattt actttgaaga cggtgacaat
     2461 gccggcgctg gcgacttctc gccccacaaa aacagccact ccgacgccga cttccgaaaa
     2521 atccctcacc gcaaccatta ctccgactga gacttccaca ccgctcccga tcgttacgcc
     2581 gacttccttt tttgtcgaca tatccactta tccattttcc gcaagtgata aattggaagt
     2641 gattgaagac attaccattc cagacggaac catgctgaaa cccgggcaaa tgtttattaa
     2701 aagctggcgg atcaagaacg taggcaataa aatctggaat aacaattatc ggcttgcatt
     2761 ggcatacagc aatcccttca ataccccgca aatgactcac gcaatcttta ttcagcccac
     2821 cgagttgatt gactttgcga tcagcacttg gaatccgcga caatataatg tgggagtgaa
     2881 tgatacggtc gatctggtta taccgcttca ggctcccgag caagccggcg attatatggt
     2941 tgagtttttt ctgattaatt cggacgatga aatcgtaccg ccaaagatct ggattcgatt
     3001 ttcggtggaa ttattgccgc aatttgcgac ccaaaccgcc gttgcgatcg cagaattaac
     3061 tgagacggcg actcctccct ccgaaagagt ccgcgttccg acacccactc cccaaccttt
     3121 cgactggacc ggaacctggt tgatccgcaa tccttattcg gatactggca tgatcccggt
     3181 tcaggcttgg ttgacgcaat ccggagatac cgtaaccggc tttttctatg ataccgacga
     3241 agagcctgtg ctcatcgaag ggaccgtcga tcgggaaaat cagggcttta tcggaaaatt
     3301 tgctcaaccg tggcagaatc gggcggttcc ggtaaaatgg cgcatgctga ttaatcgcaa
     3361 ccagttttat gcggtcaccg acagcggtga aacggaattc ggcacgattt gcggcgggcg
     3421 caacgggatg gcttttcccg aacattgttc gatgccgccc ggaatttgag gtgctgattc
     3481 aatgatttgg attccgaacg aaaataccga ccccgcgttt aaccttgccg ccgaagaagt
     3541 tattctgagt tcattgaata tcaaagaaga aacccttttc ttttatatta atgagccctc
     3601 gattattatc ggcagacacc agaatacccg ggaagaaatc aacgccgaat tcgttggcga
     3661 acagagaatc cgtattgttc gccgcctgtc cggaggcggt gcggtttatc acgatttcgg
     3721 aaacctctgt tatagcttca tttatccttt cccggatcga ttaacgccgg attttgcgct
     3781 tttcaccgca ccgattgtcc aagcattgca aaatttaggt gtaccggctg aattgagcgg
     3841 aagaaacgat attctggttg ccggcgcaaa aatttccgga aatgcatatt accataatcg
     3901 attcggatcg gtctgtcatg gcacattgct tttcgattca gatatgggca tgctcgaacg
     3961 ggcactccgg ccgaaacccg aaaaaattga acggaaaggg gttcggtcag tccgtagccg
     4021 tgtaaccaat ctgaaagatt acttaccgga cataaaagat gtaaaggaat tacgtcaggc
     4081 gattttatat tcgatttctc ggactcagcc cgacttcaaa gaatttcgac tcgcagaaaa
     4141 tgatatcctt aaaatcgaat ccttggccga atccagatat cgttcggatc ggtggaactt
     4201 cggaaaatcg ccaaaatata attatcgggt atgcaggcaa ttcagcttcg gagaagtgga
     4261 tgcccgttta tgggtggaag acaatcgcat ccgagaaata cgatttttcg gtgatttctt
     4321 cagttccgag gatccggata gtttttccga gaaattaatc ggaatcccgt ttgaatctca
     4381 ggcgatcgct caagttgcac aacaatccga catggaaaag ttattcccgg gcatatccgc
     4441 cgttgaattt gccgatttct tgaccggcaa acgcagttag gatgattttt ttatactcac
     4501 aatttaacgg agttgtcgta aataaaggat gttgagttcg cggttaatcg tttcgaatcc
     4561 caccacccac aaaaggcacg aagatcacat ttccgacatc tttccgggtt aatttcccat
     4621 tttccttgac caatttccag agagtctgac ttctgttccg cggaccgatc ggaatcaata
     4681 aaattcctcc ttcatccaat tgttcaatta acggcggagg aactttttca gccgccgcgg
     4741 taaccacgat cgcatcatat ggcgcatatt ccggccagcc gtaatatccg tcacctaatt
     4801 ttaactttac gttggtatag cctaactcat taagaatttt tttagctctt tcgtaaagct
     4861 gtggaatgat ctccacggag aaaacatccc catatcccaa ttccgccagt aatgccgttt
     4921 gataaccgga tccggttccc acttccaaca ccttatcccc cggtttcaat tctgacaact
     4981 cgatcatttg tgccaccaac gaaggttggg aaatggtttg tccgcagccg atcggtaggg
     5041 cacaatcatc atatgcctga tccgcctcac gagacgggac aaacttatgc ctcggaacgg
     5101 tcttgcaggc tcgtaaaata tccggattct ttaaattcat atatttcgcg gcaaaatcaa
     5161 aaagttgttc ccgatccttt ttatacggat cctcttcgca agcgctattc ttctctttcg
     5221 agttcaggca acatttggaa attataaaat gaagcggaat tgcataaaaa gataggcaaa
     5281 aatttcgcaa tttttccgca ttttcgcaaa gatggaagcg ggatttcata atctttctct
     5341 ttcgataagc tgttttgtta aactaattat agggtattcg cgagcgaatc gatactttca
     5401 aaatttttga aaattattcg attgatttca aaataaaaaa atccgcttct ttcaaagcgg
     5461 atttgatttt tgttgcttta aacaa
//
LOCUS       DAOMIV010000078         5415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15309_length_5415_cov_4.552425, whole genome shotgun sequence.
ACCESSION   DAOMIV010000078 DAOMIV010000000
VERSION     DAOMIV010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15309_length_5415_cov_4.552425"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PKV59_02935"
     CDS             <1..643
                     /locus_tag="PKV59_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQP45739.1"
                     /translation="VRLAPLISAVGASIFLETAAQIMFGPNKKTYKNPEILSRGSGLN
                     INMEDGMIIITKTGIFSLVLAILLMIALYFFVQKTKLGKSIRAVSQDKATAQLMGINL
                     DRVTSQTFLISGFLAGAAGVMWGLHNGIFNHYVGFLPGIKAFTAAVLGGIGNIPGAMA
                     GGMILGIVESVGPAFLGIDFQLKDVISFTILILVLILKPSGLFGKSASNVEKV"
     gene            640..2220
                     /locus_tag="PKV59_02940"
     CDS             640..2220
                     /locus_tag="PKV59_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45740.1"
                     /translation="MMNKLLSKAVKNGLVAGVILIFFEMINFTSTIAVILEKMFVSDK
                     KVKEVNPTYMIVIIGLILFLSTASFVRKNHLANEKSAVIKVLVSGLVSGLVSGILVST
                     LNAILGNYLNNGVDLRKYLDALSPDVIRNFLFQTDINNAIRIYMATTVGGAVLGAFAS
                     IVIQKASKLNGVFRSLKKGYNNLQIQKKCEKHPIIKKLFIVFVVIFIFALPMIINAYT
                     ISVLGIVFIYAILGLGLNLIVGLSGQLVFGYAAFYAIGAYTFALLTAPKPLGIEMSFI
                     PAFIIALISSGIGGLLVGLPVMNLRGDYLAIVTLGFAEIIRILINSNILADFTGGPQG
                     VKDIGQPHQPAFLVKILGKPLEENVWFLYLIIISLLVVIYIVYRLQYSRIGRSWEAMR
                     EDETVAQACGVTTSFYKVLAIVIGAIIAGAAGAIYVSRNTYTGPNEYAFMVSVNALAV
                     VVIGGMGSIPGALLGAFIIKGLPELLRDLDNYRMLVFGALLIIMMVIRPEGLLPAKRR
                     KLAVKPQSLEVLKEDLKP"
     gene            2217..3050
                     /locus_tag="PKV59_02945"
     CDS             2217..3050
                     /locus_tag="PKV59_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45741.1"
                     /translation="MTRKPPYVLEAQNITITFGGLTAVNNVSLQVPYQSIISIIGPNG
                     AGKTTFFNCISGFYSINSGIVLFQERPINGIPTDEIADMGISRTYQNIRLFSNMTVME
                     NVMIGQQPRLHANCLDAVLQTYRNRREEKQSIQKASNLLEFVGLSAYADDLAKNLPYG
                     AQRRLEIARALGNNPHLLLLDEPCAGMNPHETKAMIELINTLRSELKITIVLIEHDMK
                     VVMNISDSILVLDHGERIAEGSPEEIQKDQRVIEAYLGSGTKNVIEKYSKRKSEKVHQ
                     S"
     gene            3064..3813
                     /locus_tag="PKV59_02950"
     CDS             3064..3813
                     /locus_tag="PKV59_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45742.1"
                     /translation="MYFEIKDLHVHYRAIHALKGISLTVQKGEIVALIGGNGAGKSST
                     LNSISGLVQTSGGSIIYQDEELIGLGTDIIVQKGIVQVPEGRHIFPKMTVQENLEMGA
                     FLNNNKASVNRDMENIFQLFPRLKERRNQPGGTLSGGEQQMLAIARGLMGHPDLMLLD
                     EPSMGLSPILVEQIFDIIFDINQRGTSILLVEQNAQMALAIADRGYVLETGEIVLNGT
                     GEELMNDPKVQAAYLGGESEEEKRNKGEYYF"
     gene            4040..4933
                     /locus_tag="PKV59_02955"
     CDS             4040..4933
                     /locus_tag="PKV59_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45743.1"
                     /translation="MSNFSCKLFDNLILKSPLILASGISGNSDGLLVRAAKSGAGAVT
                     TKGVTLKYQKGAENPVSTDWGPGLITNLGLSNPGIESMAYMIKTFRNEMGDQTIPIFV
                     NIHGESAEEYAEIARLIAPSHPDLLEIDLPFPDETLAREITHAVIENASGIPISVKIS
                     ADYPKIGQLAKVIEDAGANAISAINSMKGLIVDAYAAKPFLSSGVGGISGPAIKPIAL
                     YCISEIYQAVNIPVIGTGGVLTGLDMAEMMMAGATIVGIGSALYYSGYGAFSQIMNEF
                     ETFMKSEGYQSASELVGKIWR"
     gene            4938..>5415
                     /locus_tag="PKV59_02960"
     CDS             4938..>5415
                     /locus_tag="PKV59_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45744.1"
                     /translation="MEKTILNKEKRSHVFTIDRIRYENEMTSSFIMKESLEGEPGQFL
                     LLWLPGVGEKPFSIAGTHPLVVTVADVGKLTHEIHTLQPGDRVWIRGPFGQGYQAYGT
                     DALLVGGGYGASPLLFLAETLIEKGTRVQVFLGARTKDLLILKDQFEELPLKMYITT"
BASE COUNT         1524 a         1095 c         1276 g         1520 t
ORIGIN      
        1 tgttcgactc gcccccctga tcagtgcagt cggagcgtct atttttcttg aaactgccgc
       61 ccaaatcatg tttgggccga ataaaaaaac ctataaaaac ccggaaatac tttcacgggg
      121 ttccggattg aatattaata tggaagacgg gatgatcatt attaccaaaa ccggtatttt
      181 cagcctcgtc ttggcgatcc tattaatgat cgcgttatac tttttcgttc aaaaaacaaa
      241 attgggaaaa tccattcgtg ccgtttctca agataaagct accgcgcaac tgatgggaat
      301 caatttggat cgcgttacca gccaaacttt tttgatcagc ggttttttgg cgggagcagc
      361 cggtgttatg tgggggctcc ataacggtat tttcaaccac tatgtcggat tccttcccgg
      421 cataaaagcc ttcactgctg cggtactcgg cggaatcgga aacatcccgg gagcgatggc
      481 agggggaatg attttgggaa ttgtcgaatc cgtcggaccc gcttttctcg gaatcgattt
      541 ccagttaaaa gatgtgattt cctttacgat attaattctg gttttgattt tgaaaccgtc
      601 ggggttattt ggtaaatcag catcgaatgt ggagaaagta tgatgaataa attattatcg
      661 aaagccgtaa aaaacggatt ggttgccggc gttattttaa tatttttcga aatgatcaat
      721 ttcacatcca caattgctgt catcctcgaa aaaatgtttg taagtgataa aaaagtcaaa
      781 gaagtcaatc cgacttatat gattgtcatt atcggattaa ttttattctt atcgacggca
      841 tcatttgtcc gtaaaaatca tcttgcgaat gaaaaatccg ctgtgatcaa agtgctggtt
      901 tccggtttgg tatcgggact tgtatcggga atcttggttt cgacactgaa cgcaatcctc
      961 ggaaattatc tcaataatgg ggttgatctg cgtaaatatc tggacgcgct ttctccggat
     1021 gtgattcgaa attttctctt ccaaaccgat atcaataacg cgatccgcat ttatatggct
     1081 accaccgtcg gcggagccgt tttaggggca tttgccagta tcgtcatcca aaaagcgagt
     1141 aagttgaacg gagttttccg atcactgaaa aagggatata acaacctaca aattcagaaa
     1201 aagtgtgaaa aacacccgat tatcaaaaag cttttcatcg tattcgtcgt catttttatt
     1261 tttgccttgc cgatgatcat caatgcatat actatttccg tattgggaat tgtatttatt
     1321 tatgccattt taggcctcgg cttgaatttg atcgtcggat tgtccggaca acttgttttt
     1381 ggctatgcag ccttttatgc gatcggcgcc tatacctttg cgttattgac cgcgccaaaa
     1441 ccgttgggaa tcgaaatgag cttcattccg gcatttatca tcgctttaat cagttccgga
     1501 atcggaggct tattggtcgg attgccggtt atgaatctgc ggggagacta tttagccatc
     1561 gtaaccctcg ggtttgccga gattattcga attctgataa atagcaatat tcttgccgat
     1621 tttaccggcg gccctcaagg tgtaaaagat atcgggcaac ctcatcagcc tgcgtttctg
     1681 gtaaaaattt taggaaagcc attagaggaa aatgtctggt ttctttatct gattattatc
     1741 agcttgcttg tcgtgattta tattgtttac agactgcaat attcaagaat aggacgttca
     1801 tgggaagcga tgcgggaaga cgaaaccgtc gctcaggcat gcggggtcac caccagcttt
     1861 tataaagtgc ttgcgattgt gatcggagca attatcgcgg gagcagccgg agcgatttat
     1921 gtatcgagaa atacctatac gggtccgaat gaatacgctt tcatggtttc ggttaacgct
     1981 ttagccgtcg ttgtgatcgg cggtatgggt tccatcccgg gcgcgttatt gggcgccttc
     2041 attattaaag ggttaccgga attgttgcgt gacctggata attatcgtat gctcgttttc
     2101 ggtgctctct taatcatcat gatggtcatc cggccggaag ggctcttacc tgccaaacgc
     2161 agaaagttgg cggtgaaacc ccaatctctt gaagtattaa aggaggatct taaaccatga
     2221 ctcggaaacc accttatgtt ctggaagccc aaaatataac cattaccttt ggcgggctta
     2281 cagctgtaaa caatgtcagt ttacaggttc cctatcaatc catcatcagc attatcggac
     2341 caaacggtgc gggaaaaacg acatttttca attgtatttc tggtttttat tcgatcaaca
     2401 gtggcattgt tttgtttcag gaaaggccga tcaacggtat tccgacagat gaaatcgccg
     2461 atatgggaat atcacgaacg tatcaaaata ttcgtttatt ttcaaacatg actgtcatgg
     2521 aaaacgttat gatcgggcaa cagccccgtt tacatgccaa ttgtttggat gccgttttac
     2581 agacttatcg aaatcgccgc gaagagaaac aatccattca aaaagccagt aatttgctcg
     2641 aatttgtcgg cttgagtgct tatgcggatg atttggcaaa gaatctaccc tatggtgcac
     2701 aacgtcgttt ggaaattgcc cgggctttgg gaaacaatcc gcatctttta ttgcttgatg
     2761 aaccttgtgc cggcatgaat cctcatgaaa ccaaagcgat gatcgaatta atcaataccc
     2821 ttcggtctga attaaagatc acaattgtcc tgattgaaca tgatatgaaa gtggtgatga
     2881 atatttcaga ttccatcctt gtgctggacc acggagaacg aatcgcggag ggcagtcccg
     2941 aagaaatcca aaaagatcag agagtaatcg aagcttactt gggttcggga acaaaaaatg
     3001 tgattgaaaa atattcaaaa cgcaagtcgg aaaaagttca tcagagttaa taaggaaaga
     3061 tgtatgtatt ttgaaatcaa agatctgcat gttcattatc gcgcaattca tgctttaaaa
     3121 ggcatttctc tgactgtcca aaaaggtgaa atcgtggcgc ttatcggcgg aaacggagcc
     3181 ggaaaaagct ccactctcaa ttcgatatcg gggttggttc agacaagcgg cggttcgatc
     3241 atttatcaag acgaggaatt aatcggtttg ggtacggata tcatcgtcca aaaagggatt
     3301 gttcaggttc cggaaggccg ccatattttt ccaaaaatga ccgttcagga aaatctggag
     3361 atgggcgctt tcctaaataa taacaaagct tcggttaatc gagatatgga aaatattttt
     3421 cagcttttcc cgcgtctcaa agaacggcga aatcaacccg gcggtacgct ttccgggggc
     3481 gagcaacaaa tgcttgcgat agccagaggt ctgatgggac atcccgattt aatgctgctg
     3541 gatgaaccct ccatgggact ttcgccgatt ttggttgaac agatttttga tattatcttt
     3601 gatatcaatc agcgaggcac cagcatctta ctggtcgagc aaaatgcaca aatggccttg
     3661 gcgattgccg atcgcggtta tgtgcttgaa accggtgaga ttgtgctaaa cggtacaggt
     3721 gaagagttaa tgaatgatcc gaaggtgcag gcggcttatt tgggcggaga gtcggaagaa
     3781 gaaaaacgca acaaaggcga atattatttt taacggaatt ctcagtacaa aaagggagcg
     3841 gaaatttccg ctctcttttt gtaaattgaa taaaatttct tttatatgag attttcgaaa
     3901 cggatcgctc attaatcgag atcggaaaga aaagagcggc gattcaaaga tccgaattga
     3961 ttttgatccg gaaaatcacc ccttctgata gaattggttc attgactgac tctaattctg
     4021 gttataaagg accgatagaa tgagtaattt ttcatgtaag ctgtttgata atctgatcct
     4081 aaaatcgccg ttgattctgg catccggcat ttccggtaac agtgacggtt tattggttcg
     4141 agccgccaaa tcaggtgcgg gcgccgttac cacaaaaggt gtcacattga aatatcagaa
     4201 aggtgcggaa aatcctgtct ccacggattg gggcccgggt ctgatcacca atttaggctt
     4261 aagcaacccg ggaatcgaat ccatggccta catgataaaa actttccgaa atgaaatggg
     4321 agatcaaacg ataccgatat ttgtaaatat tcacggtgaa agtgcggaag aatatgctga
     4381 aatcgcacgg ttgatcgccc cttcccaccc cgatttgctg gaaattgatt taccgttccc
     4441 ggacgaaaca ctggctcggg aaattacgca tgcggtcatc gaaaacgctt ccggaattcc
     4501 gatttcggtg aaaatatccg ccgattatcc gaaaatagga caattggcaa aagtcataga
     4561 ggatgccgga gccaatgcca tctcggcaat caattcaatg aaaggattga tcgtggatgc
     4621 ttacgcggca aagccttttc tttcctccgg agtcggcgga atcagcggtc cggcgattaa
     4681 accgattgcc ttatactgta tttcggaaat ttatcaggct gtaaatattc ctgtcatcgg
     4741 aaccggcggg gtattaaccg gcttggatat ggcggaaatg atgatggcgg gagcaacgat
     4801 tgtaggcatc gggtccgccc tgtattattc gggatatggt gcattctccc aaatcatgaa
     4861 tgaattcgaa acatttatga aatccgaagg atatcaatcc gcttcggagt tagtcggaaa
     4921 aatatggcgg taaaccgatg gaaaaaacaa tcttaaataa agaaaaaaga tcacatgtgt
     4981 ttacaatcga tcgaattcgt tacgaaaacg agatgacgag cagcttcatc atgaaagaat
     5041 ccttggaagg tgagccggga caatttcttt tgctctggct gccgggagtc ggtgaaaaac
     5101 cgttcagcat cgccggcacc catcctttgg tcgtcaccgt tgcggatgtc gggaaactga
     5161 cccacgaaat tcatacttta caaccagggg atcgggtttg gatccgagga ccatttggcc
     5221 aggggtatca agcgtacggt actgatgcgc tcttggtagg tggaggatac ggtgcctctc
     5281 cgctgttgtt tcttgcggaa acgctaattg aaaaagggac tcgtgttcag gtgtttttag
     5341 gggcacgaac aaaagatctg ttaattctga aagatcaatt tgaagaactc ccgctgaaaa
     5401 tgtatataac aacga
//
LOCUS       DAOMIV010000079         5405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15348_length_5405_cov_4.137009, whole genome shotgun sequence.
ACCESSION   DAOMIV010000079 DAOMIV010000000
VERSION     DAOMIV010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15348_length_5405_cov_4.137009"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /gene="murB"
                     /locus_tag="PKV59_02965"
     CDS             <1..770
                     /gene="murB"
                     /locus_tag="PKV59_02965"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQP45745.1"
                     /translation="IIKEYQSSGKPFKVVGGLSNILFSDNGFDGLLVINQSDEISFEP
                     QEEKVLVTVDSGAELNRFVKACAEESLTGAEWASLIPGTVGGAIYGNAGAYGGDISHI
                     LRSVQFLTDNNEIVDLRNEDLGFGYRTSKFKSGELKGTILSGTFELSRGNYDEIHEKM
                     AVIAEKRQKFNFHGNGSLGSVFRNPQGNYAGKLITECGFNGKRFGNVKVMDDHGNIFI
                     TYPGVKSSEFIELIHRVHDAVWERFSIDLTTEIEIMN"
     gene            789..1634
                     /locus_tag="PKV59_02970"
     CDS             789..1634
                     /locus_tag="PKV59_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HQP45746.1"
                     /translation="MESIFKKGTDKKDEQPEISLLTMQSHKRYGTPIVSGVKNPSRKK
                     RKNARIVSAVLTVLLSCAAAAFSFLPQFRVIAPEVSGIEQVNMEEIIYYTGLRDIPIY
                     MSNPAQIRQVLLKRYPEIREMNVTVELPQTVRIEMLKRIPVIEWDFGGSKIWIDEDGT
                     ILKESPSASSDLIYVLSNSFPGAKNKNDRKFPEKFSKKMMQSVIAINQVKPQGKKLFY
                     TYDNGYGWETDYGYTLWVGIDDSELTEKMKMAESIEKYFKENEIQPDMLSLEFTQAPY
                     YRYAE"
     gene            1639..2862
                     /gene="ftsA"
                     /locus_tag="PKV59_02975"
     CDS             1639..2862
                     /gene="ftsA"
                     /locus_tag="PKV59_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQP45747.1"
                     /translation="MEKSIVVAIDVGSTKICTLIAQIEDENSIRILGVGIEPSEGIRK
                     GTVVDIPQAANSIAKSVKKAERTSGLEITNAIVSVAGTHISSINNKATVGVVGGVIDQ
                     YDVARVVEAVRAIPIPHNRVIIHLLQRTFSVDGVDGIRSPIGMYGSRLDLEAHIITAS
                     ASSIENLRQCIMTAEIDVNQWVLNPLADGEAILTDIEKQMGVVVCDIGGGTTGLAIYI
                     NGDVWHSNILPVGGGHITNDIAQAFHLPLSQAEEVKKIHGHCIPDFIGDEEYFYCRTF
                     GQDEAAKFSRKELSKIIQMRTQEIFQLVLQEIKRSGYDNLMPAGMVLTGGTSLLPGID
                     QLASDELHMPVRLAKPENITGMIDQLDSPAFSTSVGLLYWAMMMQETVGGQPKKKDVS
                     DTMDKIKKFARYLLP"
     gene            2995..4176
                     /gene="ftsZ"
                     /locus_tag="PKV59_02980"
     CDS             2995..4176
                     /gene="ftsZ"
                     /locus_tag="PKV59_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQP45748.1"
                     /translation="MENNYQNYGESFARIKVVGVGGAGCNAINRMIEEGMQNLEFIAI
                     NTDSQALLASKASQRVRIGDKATRGLGAGGNPEVGHVAAEESRDEIKEALSGADMVFI
                     TAGMGGGTGTGAAPVVAEICREINALTVGVVTRPFSFEGPRREKAALEGIANLREHAD
                     TLIVIPNDRLLQIVDKKVSLKHAFSQADDVLRQGIQGISELITIPGLVNLDFADVKAI
                     MSEGGSALMAIGTGKGDDRARAAAEMAISSKLLDITIDGARGILFNVTGGEDLTLYEV
                     NEAAAIIKESAHPDVNFIFGAVINPDMKEEIRVTVVATGYDQPLPNPTLSNSAVKYSY
                     SESLFNRRPSDSVRTNATPLTEKKDKKIPFSSNKEEDIPEFLKKDELDVPPFMRLMTK
                     G"
     gene            complement(4321..5142)
                     /locus_tag="PKV59_02985"
     CDS             complement(4321..5142)
                     /locus_tag="PKV59_02985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQP45749.1"
                     /translation="MEAEYRDLHAVVESYEKYDEDSRLSADNIRRLEFLTALVYMNKY
                     LEKGNSILDVAAGTGAYALYYAALGMQVTALDITPSYIEILKNKAANQHLQITSFVND
                     ARDLSMFSDNSFDCVFCMGPIYHLPNPEDREKVMRESLRVLKPGGLFYLAYINKHFIF
                     ALLASQNKQYLQEKWYQRIIEQGVMYSTDEDCFWTDTWFTTPGEIEKLAERYHLKKLN
                     HVAQDGVGRLLADEINSMRPEYYKRWAEYHIRTCEEPSILGISNHGLYIGRKNSD"
BASE COUNT         1593 a         1126 c         1310 g         1376 t
ORIGIN      
        1 cgattatcaa agaatatcaa tcttccggaa aacctttcaa agtcgtagga ggattatcca
       61 acattctttt cagcgacaac ggatttgacg gattgttggt tatcaatcaa tcggatgaaa
      121 tatctttcga accacaagag gaaaaagtac tcgttaccgt tgattcggga gcagagttaa
      181 accgatttgt gaaagcttgc gccgaagaat ctttgacggg tgccgagtgg gcgagcttga
      241 ttccgggaac agttggtgga gcgatttacg gaaacgccgg agcttacggc ggagatattt
      301 cccacattct tcgatccgtg caattcttaa ccgacaataa tgagatcgtc gatctgcgaa
      361 acgaagatct cggtttcgga tatcgcacca gtaaatttaa gtcaggagaa ttgaaaggga
      421 ctattctgag cggaactttt gaattatcaa gagggaacta cgatgaaatc catgaaaaaa
      481 tggcagtcat cgcagaaaaa agacagaaat tcaattttca tggaaacggt tccctcggat
      541 ccgtctttcg aaatcctcag gggaattatg ccggtaaatt aattaccgaa tgcggattca
      601 acggcaaacg cttcggaaat gtaaaagtaa tggatgatca cggaaatatt ttcattactt
      661 atcccggagt aaaaagcagt gaatttatcg aacttattca ccgggtgcat gatgccgtat
      721 gggagagatt ttcgattgat ctgacaaccg aaattgaaat aatgaattaa gaccgcagag
      781 aaaaggaaat ggaaagtatt tttaaaaaag gaactgataa aaaagatgag cagccggaga
      841 tatcgcttct gacgatgcaa tcccataaaa gatatgggac acctatcgta agcggtgtga
      901 aaaatccctc ccggaaaaag cgcaaaaatg cgaggattgt ttctgccgtc ttaactgtcc
      961 tgttgagttg tgcggctgcc gccttttcat ttcttccgca atttcgggtg attgccccgg
     1021 aagtcagcgg gatcgaacag gtgaatatgg aagaaatcat ctattacacc ggtctacgag
     1081 atattccaat ttatatgtca aatcccgctc agatccgtca agtgctttta aagcgatatc
     1141 ccgaaattcg cgagatgaat gtgacggtgg aattacctca aaccgtcaga atcgagatgt
     1201 tgaaacgcat tccggtcatc gaatgggatt tcggcggcag taagatttgg atcgacgaag
     1261 acgggacaat cttgaaagaa tcgccttccg cttcatcgga tttaatttat gtgttgtcca
     1321 acagttttcc cggagccaaa aataaaaatg accgcaaatt tccggaaaaa ttcagtaaaa
     1381 aaatgatgca aagcgttatt gcaatcaatc aggtaaaacc gcaagggaag aagttgtttt
     1441 acacctatga caacggttac ggatgggaaa ccgattacgg ctacacgctg tgggtcggaa
     1501 tcgatgattc ggaattaact gaaaaaatga aaatggcgga aagtatcgaa aaatatttta
     1561 aagaaaacga aatacaaccg gatatgctga gcttggaatt cacgcaagcc ccttattatc
     1621 ggtacgcgga gtaagaaaat ggaaaaatcg attgttgtag cgatagatgt cgggtcgact
     1681 aaaatttgca ccctgattgc tcaaattgaa gatgaaaaca gcatccgtat tttgggagtc
     1741 ggtattgaac cttccgaagg cattcgaaag ggaacggtgg tcgatattcc tcaagctgcc
     1801 aattcaatcg cgaaatcggt aaaaaaggct gagcgaactt cggggttgga aatcaccaat
     1861 gccatcgtca gtgtcgcggg aacacatatc agttcaatta ataacaaagc gactgtgggt
     1921 gtcgtcggcg gagtaatcga tcaatacgat gtggcacgtg tcgttgaagc ggtccgggct
     1981 attccgatcc cgcataaccg tgtcattatt catctgctgc aacgaacttt cagtgtagat
     2041 ggcgttgacg gaatccgctc accgatcggt atgtacggct caaggctgga tctggaagcg
     2101 cacattatca ccgcttccgc atcatctatc gaaaatctcc gtcaatgtat tatgacagcc
     2161 gaaattgacg tcaatcaatg ggttttgaat ccgttggcgg acggcgaagc gattttaacg
     2221 gatatcgaaa aacagatggg agtggtggtc tgtgatatcg gcggtggaac gaccggttta
     2281 gctatttaca tcaatggaga tgtgtggcat tccaatattc tccccgtcgg aggcggtcac
     2341 attaccaatg acatcgctca ggcttttcac ttaccgttat ctcaagcgga agaggtcaag
     2401 aaaatccatg ggcattgcat tccggatttc atcggggatg aagaatattt ttactgtcga
     2461 accttcggtc aagatgaagc ggcaaaattc agccgaaaag aactttcgaa aattatacag
     2521 atgcggactc aagagatctt tcaactggtt ctgcaggaaa tcaaacgatc gggttacgac
     2581 aacctcatgc cggcgggaat ggtcctcacc ggcggaacca gccttttacc cgggatcgat
     2641 cagttggctt ccgatgaact tcatatgccg gtgcggttgg caaaaccgga aaacattacc
     2701 ggtatgatcg accagttgga cagtccggct ttttcaacga gtgtcgggct gctttactgg
     2761 gcgatgatga tgcaggaaac ggtgggaggg cagcctaaga agaaagatgt cagcgacacg
     2821 atggataaaa tcaaaaaatt tgcacgatat cttttgcctt aataaggagg gacaaaaccg
     2881 accgctacta aaatttttta taccgtggaa agaataggac aaagtcctga gaagatcaac
     2941 tgaagtcaaa atactatgca atggtattgt caataacgga aagaagagga agaaatggaa
     3001 aacaattatc aaaattacgg tgaaagtttt gcaagaatta aggttgtcgg cgtcggcggg
     3061 gcgggatgca atgcgattaa tcgcatgatc gaggagggaa tgcaaaattt ggagtttatc
     3121 gcgatcaata cggattctca ggctttgttg gcttccaaag catcccaacg ggtccgtatc
     3181 ggggataaag ccaccagagg attgggagcg ggcggtaacc ccgaggtcgg ccacgttgcc
     3241 gccgaagaat ctcgcgatga aatcaaagaa gcgctttccg gggcggatat ggtctttatt
     3301 accgccggta tgggtggcgg aacgggtacg ggtgcggcgc cagtcgtagc agaaatatgc
     3361 cgtgaaatca acgccttgac ggtcggtgtt gtgacccgcc ccttttcttt tgaaggaccg
     3421 cgaagagaaa aagccgcttt ggaaggaatc gccaatctgc gagaacatgc ggacacctta
     3481 attgtgatcc ccaacgaccg attattgcag atcgtcgata aaaaagtctc tctgaaacac
     3541 gctttttcac aagcagatga tgtccttcgt cagggtatcc aaggtatttc cgaattgatc
     3601 acaattccgg ggctggtaaa tctggatttt gccgatgtca aagcgattat gtccgaaggc
     3661 ggatcagcgt taatggcaat cggaaccgga aaaggtgacg atcgagcaag agcagccgct
     3721 gaaatggcta tatccagcaa gttgctcgat attacgatcg acggtgcgcg cggcattctt
     3781 ttcaatgtca ccggcggaga agatctcacg ttgtacgagg tcaacgaagc ggcagccatc
     3841 attaaggaaa gtgctcatcc ggatgtcaat tttatcttcg gagctgttat caacccggat
     3901 atgaaagaag aaattcgtgt tactgtagtt gccaccggct atgatcagcc gttgccaaac
     3961 cctactttat cgaattccgc cgttaaatac agctacagtg aaagcctgtt taatagacgt
     4021 ccttcggatt cagtccgcac caatgccacg cctttgacag agaaaaaaga taaaaagatt
     4081 ccgttttctt ccaacaaaga ggaagacatc cccgaatttc tcaaaaaaga tgaactggat
     4141 gtccctcctt ttatgcggtt gatgacaaaa ggataaatca tgaggaacaa taaggtcaga
     4201 tcagagtatt tgcgtttgat ccgatctgac cttcattcct tataaaatca caggattgga
     4261 taaatcgact tttttcaggc gattctttat caatccgttc ctttgtcgaa agcggatttt
     4321 ctaatcggaa tttttcctgc cgatatataa accgtggttg ctgatcccta aaatggacgg
     4381 ctcttcgcag gtccggatgt gatattccgc ccagcgctta tagtattccg ggcgcataga
     4441 attgatttca tccgccagca aacggcctac accgtcctgg gcgacatggt ttaatttttt
     4501 caaatggtaa cgctcggcca atttttctat ttcacctggg gtggtaaacc aggtatccgt
     4561 ccaaaaacaa tcttcatccg ttgaatacat cacgccttgc tcgataattc gttgatacca
     4621 tttttcctgt aagtattgtt tgttttggga tgccaataaa gcgaaaataa aatgcttatt
     4681 gatataggca agataaaaca atccgccggg tttcaggacc cgcagagatt cacgcatcac
     4741 tttttcccga tcctccggat tcggcaaatg atagatcgga cccatgcaaa atacacagtc
     4801 gaaagaatta tcggaaaaca tcgataaatc tcttgcatca tttacaaagg aagtgatctg
     4861 caggtgctgg ttggcggctt tattttttaa tatctcgata taggaagggg tgatatccaa
     4921 agcggtgact tgcatgccta atgcggcata atagagcgca taagcgccgg tgcctgcggc
     4981 aacgtctaaa atcgaattcc ccttttccag gtatttattc atatatacca aggcagtcag
     5041 aaactccaaa cggcggatat tatccgcaga caaacgggaa tcttcatcgt atttttcata
     5101 tgattcaaca actgcatgta aatcgcggta ttcggcttcc atttttactc ctatccttgc
     5161 tctaattata cacaggtaaa cgaagcgcaa gagaatccct tctgtttcaa catcgcatat
     5221 aaaaatttgt ttcaacgaga aaaccctcgc tctttgggag aaggttagag tgagggaaat
     5281 gaaactcggc gtcccgtaaa ttgtcctgat gtaggggacg gcgtcctcga cgtcccgtga
     5341 attgttttga tgtaggggaa ggcgtcctcg acgtcccgtg aattgttttg atgtagggga
     5401 cggcg
//
LOCUS       DAOMIV010000080         5384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15424_length_5384_cov_4.496153, whole genome shotgun sequence.
ACCESSION   DAOMIV010000080 DAOMIV010000000
VERSION     DAOMIV010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15424_length_5384_cov_4.496153"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PKV59_02990"
     CDS             complement(<1..1292)
                     /locus_tag="PKV59_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45750.1"
                     /translation="MIDSEIQPGEQEILEQCGPEILFEWQTTPPCDHIIENQNCTGLF
                     LRRTGPIISQNEIPEEKSQDSIGSFPPLRFELVDCLPSQLCQQIPEIQIRMVDSSAFT
                     SFPIKIRIRMGTFEGECESSECRVPLRMTGEQGEWLYYWTMNSNGVQSVHHRVKYRVL
                     PDRSDPTKYHFDLLSRSYPDFIPPGSDLWGLFPASYKPVSKMLIKPDSKDDLITNKPY
                     DILCGKMIWHGLVDTSHCPGFGLMGGESANACGLESCSGKLFEWQNKQNDRFYETGKK
                     YNVPPRLVKGMVAQESQFWPESDIEGEYGLGRITILGIKMLLDWYPAYFNQLCYAIFK
                     MQPNRCGSCFSEMETKDQNVLIGSLIAKTNSAEEIDLITAAVKASASQIEQIILNTSE
                     FPLEEITDYESLWKIAVANYYAGSGCVQRAIASTYLYG"
     gene            complement(1719..2534)
                     /gene="murI"
                     /locus_tag="PKV59_02995"
     CDS             complement(1719..2534)
                     /gene="murI"
                     /locus_tag="PKV59_02995"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQP45751.1"
                     /translation="MNKSAPIGIFDSGVGGLTVMRKLIQGMPEENIIYFGDTARVPYG
                     NKSKETIIHYTRQIVRFLESQNVKAIVIACNTASAYALDDIRKETEIPIIGVIEPGAY
                     AAAAATRNGKIGIIGTYATIHSGAYESFLSQINPDFEIYKQACPLFVPFVEEGLFEDD
                     LTDQIIARYLTEIKRENIDTLLLGCTHYPLIKTAIERFMGENVICVDPGLNTAEMLRQ
                     ILTKNDLLNTSFSQEKANYLFYVSDPAARMTEFANQIMPFSIGKIQKINIEEY"
     gene            2733..4031
                     /locus_tag="PKV59_03000"
     CDS             2733..4031
                     /locus_tag="PKV59_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQP45752.1"
                     /translation="MEPGSCTPYEFNTHYLTFLIVFLFLYFLFTVIYSVFRFNRRPDI
                     FSEENEKEKDTENASALSPEQVNVIWLAAKINAVIVSIFAFILINDSFCLYRSQWLLL
                     LSIILIPLVLYFLEALISGIAARNADSWIKYFHGIARLTVFFFKPLYSVYESFKPDNI
                     RNDYTFNEVEAHLREWVNNAPDNAALKEDERKMVRSILHFSDTLIREIMIPRIDMTAV
                     DVETSLEEATSIVLASGHSRLPVYEDDIDNIIGVLYAKDLLKIYTENPENRALRDYLR
                     PALFVPESKKAGDLLSEMQTSGIHMVIVVDEYGGTAGIVSMEDIVEEIVGEIRDEYDD
                     SEEKLINEISENEYSLLGRIDLEDVNEILGTHITRESADTLAGFLYSQIGKVPVGDEE
                     IEVEGWIFRIEELSGNRIRRVHVKKLEEDSPNKEGEQDEE"
     gene            4021..4425
                     /gene="cdd"
                     /locus_tag="PKV59_03005"
     CDS             4021..4425
                     /gene="cdd"
                     /locus_tag="PKV59_03005"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQP45753.1"
                     /translation="MKSENDLRELIDIALAVRGKAYAPYSKYAVGAALRTGSGKIYTG
                     VNVENSSYPLTICAERNAIFHAITEGDSDFETIVVATENGGSPCGACRQVMAEFALDM
                     NVVMVDKSGKIILQATVDELLPEAFTPKSLRK"
     gene            4432..5181
                     /gene="recO"
                     /locus_tag="PKV59_03010"
     CDS             4432..5181
                     /gene="recO"
                     /locus_tag="PKV59_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQP45754.1"
                     /translation="MERSVRVQALVLSHNDFGEADRYVKLLTPDRGKISALAKGVRKM
                     NSRKAGHLEPFTIISAQLVQGKGSSWIISQVSTIESFPGLTDNLESTCHAAYVSELTD
                     RFATEEYSNFELYKLAVETMRRLSKITDHFPVLRYFDFRLLDLAGFRPQLHSCVRCGN
                     EIIEEQQFFSNILGGVVCPKCGMKESGIVSISSRSLKYFRYYQTHNFSQAAAAGWPAD
                     LRQESEIILTNYLTYFAEKKINSQIFLNCIR"
     gene            5230..>5384
                     /locus_tag="PKV59_03015"
     CDS             5230..>5384
                     /locus_tag="PKV59_03015"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45755.1"
                     /translation="MRYGTFDDEKLEYCITQPDTPLPWINYLGTEDFFGLISNTAGGY
                     CFYRDAR"
BASE COUNT         1547 a         1064 c         1159 g         1614 t
ORIGIN      
        1 tatccgtaaa gataggtgct cgcgattgcc ctttggacac atccggatcc ggcataataa
       61 ttggcgaccg caattttcca taaactctcg taatccgtaa tttcttctaa cggaaattcg
      121 gaagtattca gaattatttg ttcaatttgg ctggcacttg ccttgacagc cgcggtaatc
      181 agatcaattt cctcggcaga atttgtttta gcgatcaggc ttccgatcaa cacattttga
      241 tcttttgttt ccatttcgga gaagcagctg ccacagcgat tgggttgcat tttaaaaatt
      301 gcatagcaga gttgattgaa atatgccgga taccaatcca ataacatttt tatccccagt
      361 attgtgattc gccccaatcc atattcccct tcgatatctg attccggcca aaactgagat
      421 tcttgagcta ccattccttt taccagtcgg ggaggaacat tatatttctt tccggtctca
      481 tagaaccgat cattctgctt attttgccat tcaaataatt ttccggaaca agactcaaga
      541 ccacaggcat ttgctgattc tcctcccatt aatccaaagc caggacaatg tgacgtatca
      601 accagtccgt gccaaatcat tttgccgcaa agaatgtcat atggtttatt tgtaatcagg
      661 tcgtctttcg aatccggctt tataagcatc ttggaaaccg gtttataact cgctggaaat
      721 aagccccata gatctgatcc gggaggaata aaatccggat aagaacggct taatagatca
      781 aaatgatact ttgtcggatc cgatcgatcc ggtaaaaccc gatatttcac ccggtgatga
      841 acactttgca ctccgttcga gttcattgtc cagtaataaa gccattcccc ttgttcaccg
      901 gtcatacgaa gcggtaccct gcattcggaa ctttcgcatt ctccttcgaa agttcccatc
      961 cgaatccgga tctttatcgg gaaagatgta aaagcggatg aatcgaccat tcgaatttga
     1021 atttccggta tttgttgaca gagttgactt ggcagacaat cgaccagttc aaatcggagc
     1081 ggtgggaaag acccaatgga atcctgggat ttttcttcgg gaatttcatt ttgtgaaatg
     1141 atcggtccgg ttcttcgaag gaacagaccg gtgcaattct gattttcgat gatatgatca
     1201 catggcggag tcgtttgcca ttcgaaaagg atttcgggac cgcactgttc taaaatttcc
     1261 tgttctccgg gctgaatttc ggaatcgatc atcagtgtac aaactgttag agaggtattc
     1321 caagaggcaa gttgccaaac gatttgttga tcggatgatg ctgcaaatat agagattgaa
     1381 gtacaaaaaa tcaggaagat aaagaacgaa acagaaagaa gaagaaagaa ggatatcctt
     1441 ttctttcctc tcataaaagg tcactcgttc ttcgttacga gatcatttga ttttagaagc
     1501 atgattctat ttttgatcta tctatattta tgatatgaaa tgatcgatcc gataaaacct
     1561 ggaaaattat actcggaacg tacttgaatg aacagggaaa tggaatttaa tgcggcaagt
     1621 taaagaataa tagaaaatac cagaataaaa agactttttc tgaagaatat ttgataagat
     1681 tgataagcaa agttcccgtt gaagacttga gaatcagact aatattcttc gatattaatc
     1741 ttttgtatct ttccgataga aaaaggcatg atttgattgg caaattcagt cattcttgca
     1801 gccggatcgc ttacataaaa caaataattt gctttttctt gagagaaaga agtatttaga
     1861 aggtcatttt tcgtaagaat ctgccgaagc atttcggcgg tgttcaaacc gggatctaca
     1921 caaattacat tctctcccat aaatctctca atggctgtct taattaacgg ataatgcgta
     1981 catcctaaca gtagggtatc gatattttct ctcttaattt ccgttaggta acgggcaata
     2041 atctgatcgg taagatcatc ctcaaaaagt ccttcttcaa cgaatgggac aaataaagga
     2101 caagcttgtt tataaatttc aaaatccgga ttaatctggc ttaaaaagga ttcgtatgca
     2161 ccggaatgta tcgttgcata ggtaccgata ataccgattt tcccatttcg ggttgccgct
     2221 gccgctgcat aagcacccgg ttcgatgaca ccgatgatcg gtatttcggt ttccttccga
     2281 atatcatcta atgcataagc gcttgctgtg ttgcaggcga taacgattgc ctttacgttt
     2341 tgactttcca gaaatcggac gatctgtcga gtgtaatgaa taatagtctc tttggatttg
     2401 ttcccgtatg gaacccgagc ggtgtcccca aaataaatga tattttcttc cggcatccct
     2461 tgaatcagtt ttcgcatgac ggtcaatccg ccgacccctg aatcaaaaat tccgatcgga
     2521 gcacttttat tcattcaatt tctctccttt tcccacttgc aaaaagcttt ttcgagcaat
     2581 ttacaaatta tacgcggaaa acttgattgc caaatttcta ataacattat cattaatgat
     2641 gtaattcaat cagttataat ttttatatcg cacatgattt tgtgtagcga tagtagaaag
     2701 gttgatgtat cggcgtaaac cggtaaaaat taatggaacc tggaagttgt actccatacg
     2761 aattcaatac ccattatctg acattcttaa ttgttttttt atttctgtat tttttgttta
     2821 cagttattta ttccgtattc agatttaata ggcgccccga cattttttca gaagaaaatg
     2881 aaaaagagaa ggatacggaa aatgcttccg ctttatctcc cgagcaagtg aatgttatct
     2941 ggttggctgc aaaaataaat gcggtcatcg tatccatttt tgcgtttatt ctgattaacg
     3001 acagcttctg cctctatcga agtcaatggc tcttattatt gagtatcatt ctgattccgc
     3061 ttgtattgta tttcttggaa gcattgatca gcggtattgc cgcacggaat gccgattcat
     3121 ggataaagta cttccatggt attgcccgtc tgacagtttt tttctttaaa ccgctttatt
     3181 cggtttatga gtcttttaag cccgataata ttcgcaacga ttatactttc aatgaagtgg
     3241 aagcccattt aagagaatgg gtaaataatg ctccggataa tgccgctttg aaagaagatg
     3301 aacgaaaaat ggtccgttct attcttcatt tcagcgatac tctgattcga gagataatga
     3361 ttcctcgtat tgatatgacc gcggtagatg tcgaaaccag tcttgaagaa gcgacatcca
     3421 ttgtattggc ttccggacat tctcgtttgc cggtttatga ggatgatatc gataatatta
     3481 tcggtgttct ttatgcaaaa gatctattaa aaatctatac ggaaaatccg gaaaaccgcg
     3541 cgctgcggga ctatctgagg cctgctttat ttgtgcccga atccaaaaag gcaggtgact
     3601 tattgagtga aatgcaaact tccggaattc acatggtgat tgttgtcgat gaatacggag
     3661 gaactgccgg gatcgtatct atggaagaca ttgtagaaga aattgtcgga gaaattcgtg
     3721 acgaatatga tgacagtgaa gaaaagctga tcaatgaaat cagtgaaaac gaatattctc
     3781 ttttgggtag aatcgacttg gaagatgtga atgaaattct cgggacccat attacgcgag
     3841 aatccgcgga tacacttgcc ggcttccttt actcccagat cggtaaagta ccggtcggtg
     3901 atgaggaaat cgaagtggaa ggttggatat tccgcattga agagttatcc ggaaacagaa
     3961 tcaggcgtgt ccatgtaaaa aaattggaag aagattcgcc gaacaaggaa ggtgaacagg
     4021 atgaagagtg aaaatgatct tcgggaattg attgatatcg cattggcagt ccgcggtaaa
     4081 gcttatgcgc cctattcgaa atacgcggtg ggcgctgcac tgcgaacagg cagcggtaaa
     4141 atctacaccg gtgtgaatgt ggaaaactct tcatatcctc tcactatttg cgcggaacga
     4201 aacgccatct tccatgcgat tactgagggc gattcggatt ttgaaacgat cgtggtagcg
     4261 accgagaatg gcggatctcc ttgcggtgcc tgtcggcagg taatggcgga atttgcgttg
     4321 gatatgaacg ttgtgatggt ggataaaagc ggaaagatca ttttacaggc cactgtggat
     4381 gaacttttac cggaagcttt cactccgaaa tcactcagaa aatagcacgt tatggaaaga
     4441 tctgttcgag ttcaggctct ggttctcagc cataatgact ttggtgaagc ggatcgatat
     4501 gtaaaattat tgacacccga tcgaggaaaa atttcggcct tggcaaaagg agtgcgaaag
     4561 atgaattccc gaaaagccgg tcatttagag ccgtttacta taatttcagc ccagctggtc
     4621 caagggaaag gatcatcttg gattatcagc caagtttcta ccatagagag tttccccggc
     4681 ttgaccgata atttggaatc gacttgccat gcggcctatg tctcggaatt gacggatcgc
     4741 tttgctacgg aggaatattc gaattttgag ctttataaac tggctgtgga aaccatgcgc
     4801 cgtctttcaa aaataaccga tcatttcccg gtgctgcgat atttcgactt cagactgctt
     4861 gatttggccg gctttcgacc gcaacttcat tcctgtgtgc ggtgcggtaa cgaaattatc
     4921 gaagagcaac aatttttttc aaacattttg ggcggagtgg tttgtccgaa atgcggtatg
     4981 aaagaatccg gcatcgtttc catttcgtct cgctcgttga aatattttcg ctattatcaa
     5041 acacacaact tttctcaagc tgcggcagcc ggctggcccg ctgatctaag gcaagaatcg
     5101 gaaatcatcc tgaccaatta tctgacctat tttgcggaaa aaaagatcaa tagtcagatc
     5161 tttttaaact gtattcgttg atcattgatc gataaaaata agagaatcac tttataaacg
     5221 gaggtaaaga tgcgttacgg gactttcgac gacgagaagc ttgaatattg tattacgcaa
     5281 ccggatacac cgctgccttg gattaattat ttggggacag aagatttttt tggattgatt
     5341 tcaaatacgg ctggtggata ctgcttttat cgggatgccc gttt
//
LOCUS       DAOMIV010000081         5299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15737_length_5299_cov_4.852593, whole genome shotgun sequence.
ACCESSION   DAOMIV010000081 DAOMIV010000000
VERSION     DAOMIV010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15737_length_5299_cov_4.852593"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1297
                     /locus_tag="PKV59_03020"
     CDS             32..1297
                     /locus_tag="PKV59_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQP45756.1"
                     /translation="MAVVGLGYVGLPLATVFAEAGYPVTGIDLDPIKTEKVNRGESYI
                     PDIETARIRKLREKKLLQATNDFGVLKDIDLVSICVPTPLRKTGDPDLSFIINAAEKI
                     APFMHRGMAVAFESSTYPGTTRELVLPKLTADNSLRVGEDFFLIFSPERIDPGRKDWT
                     TKNTPKIVGGISEKCTEIAVRWYEKALDTIIPVSSPEVAEMAKLLENTFRMINIGMVN
                     ELAMMCNRLNVDVWEVIEAAATKPFGFMKFTPGPGLGGHCIPVDPHYLSWKLKSLHYT
                     ARFIDLASEINTNMPRYTVQLIQDALNLQKKSLNGSQVLVLGAAYKPDIDDMRESPSL
                     DVMHLLMQKNAVVHYHDPYIPTLRLDDTIMNSVADLNQALTDADCVAILTNHSCYKPE
                     VLLEKSRLIVDTRNALGNAGKNNPKVVRL"
     gene            1300..2274
                     /locus_tag="PKV59_03025"
     CDS             1300..2274
                     /locus_tag="PKV59_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQP45757.1"
                     /translation="MNHIFVTGAAGFIGARICEMLSEEGIQVTGIDNFSPTYDIALKN
                     YRLNKLQQLPEFSFYPGDITDFDLLTQIFEKNKFDAVINIAGIPGVRLSVENPWLFLE
                     ANTKGALNLLEVCRRNDVGKFIQASTSSVYGKDAPFPTPEIADSSHPLQPYSSSKKGA
                     ETMAYAYHYLYGLDVTVTRFFTVYGPAGRPDMAIFRFIKWISEGTPLKVNGDGTQTRG
                     FTYIDDIAQGTILALKKVGYEIVNLGGHESVSINDLIHRLEDIIGKKAIIDYRPIFKA
                     DMLANLADVTKARTLLNWNPRINLDEGLQQTVAWHRQEQSWLQNIKID"
     gene            2279..3670
                     /locus_tag="PKV59_03030"
     CDS             2279..3670
                     /locus_tag="PKV59_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQP45758.1"
                     /translation="MIKKIRGWFSDYVFKRVVRNSAYLITSNLISVFLTIYITRLLGV
                     YNYGVLGVITSYVTNVNRFFSFRMNEMIVRYVSEPYEKMDQDQAGALIKFGALIETCT
                     SIFAFLFLLVTAQWGARIFLNDPTLVQPVLIYGITILTGFAMETANGVLRVINRYRAI
                     AIISLVQNILVAVLVLLAAKLNYGLNAILLAYLIGKVILGVVPVILVLIWLPKFIGRG
                     WWKAPISLIRDKKEILRFTLSTNISSTINLFARDGEILWVSAFLSPLYAGYYKTAMTV
                     INMILTPITPFIDTTYPEMMRAIVNNRWNRLKQLIRKVTALSTTWTVGVGLILILFGK
                     QLLYSELNFFGWRFHVFPAEYLPSYQIILILLIGYGIANILFWNRTLLLSLSKAGTAT
                     KISFYGMLLKIIATVLLVPIGPYYLESVILSGYLAGTVLIMTVIGIKGVNHAQRTVVT
                     QNEALSINDMHTG"
     gene            3724..4872
                     /locus_tag="PKV59_03035"
     CDS             3724..4872
                     /locus_tag="PKV59_03035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQP45759.1"
                     /translation="MKIAVSSTSTIPSTTANSIQVLMACQGLVQNGQEICLWIPGKES
                     PDFDLLREQYGLTCQSFPVLPIRSRKRLHRLDFSRQTEKESRKWGADCIYTWTIQVAL
                     IASRHRLPVIFEVHDLPKGTFGKRWFNEFAKSDTVKRFAFITRALEEEVCAEYPNLKK
                     SDCLIAPNGINPEEYQDLPNSHEAKKEMGFEDRVIASCSGHLYPGRGVELFLHLAREF
                     PEIKFYWFGGTADSVKYYREKAAESGLKNVIFTGFIPKSKIPLAQAASDFLLMPYEKT
                     IAGSSGGNSAAICSPMKMFEYLAAGKPILASNLPVIHEILDPTCAVFCEPENLSFWIE
                     GLKMLLGHPEEAAKLAAAAKRKSSFYSWKNREKTILDALTEGKNANKS"
     gene            4856..>5299
                     /locus_tag="PKV59_03040"
     CDS             4856..>5299
                     /locus_tag="PKV59_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45760.1"
                     /translation="MQINHKKKLPVYIFAIIFLSLLTSATLAYFSPGTFIRGFTTALI
                     FSVPSYLILYFLWKWGGAGKTLAVMMFCSIFLRLGFGLATQNLLPIYGYRDEPQQQGG
                     YLSNDVYRRDEETWLIAQNEKPGFIETITRDYNNDQYGGLAALSVW"
BASE COUNT         1450 a         1186 c         1234 g         1429 t
ORIGIN      
        1 gaagaataag atcgaaaaaa acgaagcccg aatggctgtg gtcgggctgg ggtatgtcgg
       61 gctgcctttg gcaaccgttt ttgcggaagc tggttatccc gtaaccggta tcgatttgga
      121 tccgatcaaa acagagaagg ttaatcgggg cgaaagctat attccggata tcgagacagc
      181 cagaattcgg aaactgcggg aaaagaagct gcttcaagcc acaaacgatt ttggtgtatt
      241 gaaagacatc gatctcgtca gcatttgcgt cccaactccc ttgcgaaaaa caggggatcc
      301 ggatttatct tttattatca acgccgccga aaaaatcgct ccgtttatgc atcgcggaat
      361 ggccgtcgcg tttgaatcca gtacctaccc gggtaccacc cgcgaactgg tcttaccgaa
      421 actgacggct gacaattccc tgcgggtggg ggaagacttt ttcctgattt tttcaccgga
      481 acgaatcgat cccgggcgga aggattggac aacgaaaaac actcccaaaa tcgtcggggg
      541 aatcagtgaa aaatgcacgg aaatcgcggt ccgttggtat gaaaaagctt tagatacgat
      601 catacccgta tcctctccgg aagtagcgga aatggcaaag ttgcttgaaa acacatttcg
      661 gatgatcaat atcggaatgg tcaacgaatt ggctatgatg tgcaaccggc tcaacgtcga
      721 tgtctgggaa gtgattgaag ccgccgcaac aaaaccgttc ggatttatga aattcactcc
      781 cggacccggc ttggggggac attgtattcc agtggacccg cattaccttt cttggaagtt
      841 gaaatctctc cattacaccg cgcggtttat cgatcttgcc tcggaaatca acaccaatat
      901 gccgcgttac accgtccaac tcatccagga tgccttaaac ttgcaaaaga agagcttgaa
      961 cggctctcaa gtattggttt tgggagcggc ttacaaaccg gacattgacg atatgcggga
     1021 aagcccgtct ttggatgtaa tgcatcttct catgcagaaa aacgccgtcg ttcattatca
     1081 cgatccttat atcccgacat tgagattgga tgatactatc atgaatagcg tagcggatct
     1141 gaatcaagct ctgacggatg cggattgtgt tgcgattctc accaatcact cctgttataa
     1201 accggaagtc ttactggaga aaagccggtt gattgtcgat acccgcaacg cgctcggaaa
     1261 tgccggaaaa aataacccca aagttgtcag gttataaaaa tgaaccatat tttcgtgaca
     1321 ggcgccgcgg gatttatcgg tgcgcgaatt tgcgaaatgt tgagcgaaga gggcattcaa
     1381 gttaccggta tcgataattt cagtccgact tatgatattg cgttaaagaa ctatcgtctg
     1441 aacaaacttc aacagctgcc tgaattttcc ttttatcccg gggatatcac agattttgac
     1501 ttactgacac agattttcga aaagaataaa tttgatgcgg ttattaatat tgccgggatt
     1561 ccgggagtgc ggctgagtgt cgaaaatcca tggttgtttt tggaagcgaa taccaaaggt
     1621 gctttaaatc tccttgaagt ctgccgccgc aatgatgtag gaaaatttat tcaagcttcc
     1681 acatccagtg tctacggaaa ggatgcacct ttcccgacac ccgaaatagc cgattcaagc
     1741 catccgctcc aaccgtattc ttccagcaag aaaggggcgg aaaccatggc ttatgcttac
     1801 cattatcttt acggactgga tgttaccgtc acacgcttct ttaccgtcta tgggcctgcg
     1861 ggacgtccgg acatggctat tttccgattt atcaagtgga tatcggaagg aacacctctt
     1921 aaagttaacg gagacggtac ccaaacccgc ggctttactt atatcgatga tattgctcaa
     1981 ggaacaattt tggcattaaa aaaagtcgga tacgaaatcg taaatctggg tgggcatgaa
     2041 tcagtttcga tcaacgattt gattcatcgg ttggaagaca taatcggaaa gaaagcaatc
     2101 atcgattatc gaccgatctt caaagcggac atgcttgcca atctggcgga tgtgacgaaa
     2161 gctcggacat tgctgaattg gaatccgcga atcaatctgg atgaaggatt gcaacaaacg
     2221 gtcgcctggc accggcagga acaaagctgg ttgcaaaata tcaaaatcga ttgactttat
     2281 gatcaaaaaa atacggggat ggttttcgga ttacgttttt aaacgagttg tgcggaactc
     2341 tgcttacctg attaccagca acctgatcag cgtttttctg acaatctata tcacccgatt
     2401 gttgggtgtc tacaattatg gtgtcctcgg agtgattacc agttatgtaa ccaatgtcaa
     2461 tcggtttttc tctttccgca tgaacgagat gattgttcgc tatgtcagtg aaccatatga
     2521 aaaaatggat caggatcaag cgggagcgtt gatcaaattc ggcgctttga ttgaaacttg
     2581 tacttcgata ttcgcttttc tttttctgct tgtgacagcg caatggggtg cccgtatttt
     2641 tctaaatgat cccacattag tccaaccggt ccttatttac ggaattacca tcttgaccgg
     2701 ttttgcgatg gagacagcca acggtgtttt acgggtcatc aatcgctacc gggcgatagc
     2761 gattatcagc ttggttcaaa atattctggt agctgttctg gttcttttag ccgcaaaatt
     2821 aaattacggt ttaaatgcca tcctacttgc gtatttgatc ggaaaagtaa tcctcggggt
     2881 tgtcccggtc atattagtgc tgatctggct gccgaaattt atcggaagag gctggtggaa
     2941 agctccgata agcctgattc gggataaaaa agaaatcctg cgttttaccc tttccaccaa
     3001 tatcagcagt accataaacc ttttcgcaag agacggggag atattatggg tcagtgcctt
     3061 cttgagcccg ctgtatgccg gatactataa aacggcgatg acggtaatca atatgatatt
     3121 gacacccatc acaccgttta tcgatacaac ctaccctgaa atgatgcgag cgatcgtcaa
     3181 taatcgatgg aatcgcctga aacaactgat tcgaaaggtg acagccctct caacgacatg
     3241 gacggtcgga gtcggtctga tattaatcct gttcgggaaa cagttactct acagcgaatt
     3301 gaactttttc ggttggcggt ttcatgtttt ccccgccgaa tatctgccat cctatcaaat
     3361 catcctgatt ttgctgatcg gatacggtat agcaaacatt cttttttgga atcgaacgct
     3421 tttactttct ctaagtaaag caggaacggc aacgaaaatc agtttttacg gtatgttatt
     3481 aaaaatcatc gcgaccgttc ttctcgttcc cataggtcct tattatttgg aatcggttat
     3541 tctttccgga tatctggccg gaacggtttt gatcatgacg gttatcggca tcaaaggggt
     3601 taatcatgcc caacggaccg ttgtcacgca aaacgaagcc ttgagcataa atgatatgca
     3661 taccggttga tcggtaccgg ttccgtaatc tttccgaata ttgaaccaga gtaaaatgag
     3721 tgtatgaaga tcgccgtcag ttccacctca accatcccct ccacaaccgc caattccatc
     3781 caagttttaa tggcgtgtca gggattggtt caaaacgggc aggagatctg cttatggatt
     3841 cccgggaaag aatcacccga ttttgacctg ttacgggaac agtatggatt aacttgtcaa
     3901 agcttccctg ttctacccat ccgatcaaga aaaaggttgc accgattgga tttcagccgg
     3961 cagacggaaa aagaatctcg caagtgggga gcagattgca tctatacctg gactatccag
     4021 gttgcgctta ttgcctcccg gcatcggttg cccgtcattt tcgaagtgca cgatttgccg
     4081 aaaggcactt tcggtaaacg atggttcaat gaatttgcaa aatccgatac ggtgaaacgc
     4141 ttcgctttta tcacacgggc gttggaggaa gaagtctgtg ccgaatatcc caatttaaag
     4201 aaatcggatt gtctgatcgc acccaacggt attaatccag aagagtatca agatcttccg
     4261 aatagtcatg aagcgaaaaa agaaatggga tttgaagatc gagtcatcgc ttcctgcagc
     4321 ggtcatttgt acccgggaag gggcgtggag cttttccttc accttgcccg cgaatttccc
     4381 gaaattaaat tttactggtt cggcggaacg gcggattccg tgaaatacta tcgggaaaaa
     4441 gcagctgaat cgggattgaa aaatgtaatc tttaccggtt ttattcctaa atccaaaatc
     4501 ccgctggcac aggcagcaag tgatttttta ttgatgcctt atgaaaaaac aatcgccgga
     4561 agcagcggcg ggaattccgc cgccatttgc agcccgatga aaatgtttga atatttagcc
     4621 gccggtaaac cgatcctggc ttccaatctt ccggtcattc acgaaatttt ggacccgact
     4681 tgtgccgtat tctgcgagcc ggaaaacctc tctttttgga ttgaagggtt gaaaatgctc
     4741 ctcggtcatc ccgaagaagc ggcaaaactc gccgctgcgg caaagcggaa atcttcgttc
     4801 tatagttgga aaaacagaga aaaaacgatc cttgacgcgt taacggaagg aaaaaatgca
     4861 aataaatcat aaaaagaaac taccggtata tatcttcgca atcatttttc tttcacttct
     4921 tacctcagct acattagcct atttcagccc gggaacattc atccgcgggt ttaccactgc
     4981 gctgattttt tccgtccctt cctatttgat tctttatttt ttatggaaat ggggaggcgc
     5041 agggaaaaca ttagccgtca tgatgttttg ttccattttt cttcgccttg gattcggctt
     5101 ggcaacccaa aatttgttac cgatatacgg ttaccgggat gaaccgcaac aacagggtgg
     5161 ttacctttcg aatgatgttt atcgacggga tgaggagacc tggctgattg cacaaaatga
     5221 aaagcccggt tttatcgaga cgatcacccg agattataac aacgatcagt acggcgggct
     5281 ggctgccctt tccgtctgg
//
LOCUS       DAOMIV010000082         5288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15791_length_5288_cov_4.557615, whole genome shotgun sequence.
ACCESSION   DAOMIV010000082 DAOMIV010000000
VERSION     DAOMIV010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15791_length_5288_cov_4.557615"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PKV59_03045"
     CDS             complement(<1..554)
                     /locus_tag="PKV59_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HQP45761.1"
                     /translation="MMRFDRFTESAQDAAQRAVEILQRYGQNQIDTEHFLLALIEQPQ
                     GTVSQILDEMNVSTAVLSDRLDYMLRTSTKTNIYGGGSGQIFITPNVRRIVDQAYIEA
                     NRLKDEYTSTEHIFLAILNEKGTPVAKLMEEFGITRAKVYEIVVKMHDNKNAPVQNKD
                     SRYKNLQKYSRDLTDQAREGKLDPV"
     gene            complement(724..1425)
                     /locus_tag="PKV59_03050"
     CDS             complement(724..1425)
                     /locus_tag="PKV59_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45762.1"
                     /translation="MDPEVFLDLYNNLETKLKGRGSYYNDRYESPIIRFSNSSEGKPY
                     REELSTIRDIRNLLVHSPLVNGKYPVEPSDEIGAVLEEILEKVEQPKKAMNYATWADK
                     IQKTRLDEEAYPVMRMMELRGFSHVPVVEGEKMIGVFSKSTVFSFHIRNNLTTIQKGM
                     RIKEFGNLLDIENHSNEYFEFAPKDALYTDILELFNTNYKKRQKRLAIVFLTETGNSQ
                     EKLLGLITPWDVLGK"
     gene            1602..2276
                     /locus_tag="PKV59_03055"
     CDS             1602..2276
                     /locus_tag="PKV59_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45763.1"
                     /translation="MSKKQKNNKNSKQNAGDLFGLAMWIFAAFGLEIVLIFIETKIYA
                     VSSLNWTIRQNCLHWALISLFWAIISFLIVRSAKKDYDFDILKRQQNSTLTQILFSVL
                     LVIVGILFVSYMSGGFKPVSEYKKLGSVQFIFQYITYFFKSILVMLAIALGQKAGDLY
                     FQKDQIPWGGYFLAVSWGFIQFIIQNPATGLFTVALSVLFGVLFMINHKNARIVFPMI
                     AIVLML"
     gene            2612..3742
                     /locus_tag="PKV59_03060"
     CDS             2612..3742
                     /locus_tag="PKV59_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45764.1"
                     /translation="MKKSALLAIVVLCLAVSTGVLGFMYSTKGDKTAEEAAPMVQIGV
                     MTNKWAASAHGDSSSEAFRHWDKEGEVPVTCAKCHSSAGMQDYLGADGTAAMTVDNPA
                     KVGSVVDCSACHNEVIQNTTSVVFPSGKEVAAEPSYASCWTCHQGTEAGINGSLDQKI
                     GSTAPDDKGENLGFVNPHYLNVAGIYLGSEANAGYQYEGKTYAARFEHASGVQTCTEC
                     HDPHSLHLADPGLEKCSTCHAEVETWPDQQKIRRTKEDLDGDGNTDESTYEEINGLLE
                     RELQAMVTYSVDVAKVQIGNSDVYPYWFADKNGNGTQEEDEKQYADWTPRLMYAAYNF
                     KLVKTSAGYIHNPTYSAQLLIDSISDLAAGDSAISVDGIERP"
     gene            3761..5134
                     /locus_tag="PKV59_03065"
     CDS             3761..5134
                     /locus_tag="PKV59_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HQP45765.1"
                     /translation="MNTAASRSARRAYIAQLRVKRIFWICLFVVFALFPLVVAAINNT
                     TNGTWRAVPREIGSFAGMLGLSLMILSFIPIMREKKITSAFNLDVVYKFHHSCSILGF
                     WLVVFHLVALWINNQEIGRLVNIFADYPLYMKAGPIALLAAGCLVYFSYFRKELKLNY
                     DYWKIGHSLLAILMVVFGLIHTFGVNYYSGNALIHGYYVFLTVLAAIAILWLRVFSSL
                     SAMCKPYVIREVLIRNNATTELRLEFAGKGNQKPIRYHAGQIAWITVRHSRFSFKEHP
                     FSIASSDIDQNKIGFAIRELGDFTATIKDLKPGEIVYVEGGFGIFNPYILGDEGFVLI
                     AGGIGIAPVMGILRSMADRKDRRHVVLYYGSRDKESIGFYDELNELSKKLNLEVVHVL
                     EKTDDPSFEKGYVTKDVIERHYPKNKDRYDVFICGPGPMVQIVLPALKKIGIPKENIW
                     PEFYDMA"
BASE COUNT         1439 a         1032 c         1179 g         1638 t
ORIGIN      
        1 accggatcca attttccttc tcgagcctga tcggtcagat cacgggaata tttttgcaga
       61 ttcttatatc tggaatcttt gttttgtacc ggagcatttt tgttatcgtg catttttaca
      121 acaatttcat agactttggc ccgtgtaatg ccgaattctt ccattaattt tgcaacgggt
      181 gtcccttttt cgtttagaat cgctaaaaaa atatgttcgg tagaggtata ttcgtctttc
      241 agacgattgg cttcgatata cgcctggtca acaattcttc gaacattggg cgtaatgaat
      301 atttgtccgg atcctccgcc gtaaatgttg gtctttgtac tggttcgaag catgtaatct
      361 aatctgtctg acagaacagc ggtggaaaca ttcatctcat ctaaaatttg agaaacggtc
      421 ccctgcggct gttcgatcag cgccaataaa aagtgctcgg tatcaatttg attttgtccg
      481 taacgttgaa gaatctcgac agcgcgttgg gctgcgtctt gagcactttc tgtaaatcgg
      541 tcaaatcgca tcataatttc tttccatctt tctcgttaaa tcgaaccgaa aaatcagttc
      601 gaatcaccgt tttaatcgtt catttattgc ctaaaaaggt caacgtgagg ttattgtagc
      661 atttcactgt aagaacatta ttagatttac gcaagagtca ggtctgagca gacgcatgga
      721 attttattta ccgagtacat cccaaggggt gatcaaaccc agcagtttct cttgagagtt
      781 gcccgtttcg gtcaggaaaa caattgccaa tcttttctgc cgttttttat agttggtgtt
      841 aaaaagttca aggatgtccg tatacaaggc atctttcggc gcaaattcaa aatactcatt
      901 cgagtgattt tcaatgtcta gcaaattgcc gaattcttta attcgcattc ctttttgaat
      961 cgtagtcaga ttgtttcgga tatggaaact gaagacggtg cttttgctga aaactccgat
     1021 cattttctca ccttcgacaa ccggaacatg agaaaatcct cttaattcca tcatacgcat
     1081 cacgggatag gcttcttcgt ctaaacgggt tttttgaatt ttatccgccc aagttgcata
     1141 gttcatggct tttttgggct gttccacttt ttccaagatt tcttctaaaa ccgcgccgat
     1201 ttcatccgac ggctcaaccg gatatttgcc gtttaccagc ggagaatgga cgagcaaatt
     1261 tcgaatgtct cgaatcgtcg acaattcttc ccggtatggt ttcccttcac ttgaattcga
     1321 aaagcggatg atggggcttt catacctgtc gttataataa cttccgcgac ctttcaattt
     1381 tgtttccaga ttgttgtaga gatcaagaaa aacttcggga tccatgcatt ttgtcctttt
     1441 tcggttattt ggcttttatt tgattatatc tttatgattt tctgttttca gaaatattta
     1501 ggcttttatc aggttgattg ttataaatgc tcaagtttga aattataatt caacaggttc
     1561 gacgtaattt attgaataaa aagggaaata gagcctttgt tatgtccaaa aaacaaaaaa
     1621 ataataaaaa cagcaaacag aatgcgggcg atctattcgg attggctatg tggatttttg
     1681 cagcattcgg attggaaatc gttttaatct ttatcgaaac caaaatttat gcggtttctt
     1741 ctttaaattg gacgataagg caaaattgtc ttcattgggc actgatcagc cttttttggg
     1801 cgattatctc tttcttaatc gtacgcagtg ccaagaaaga ttatgacttt gatattttaa
     1861 aaagacagca aaactcgact ttgacacaaa ttcttttttc cgtccttttg gtaattgtcg
     1921 gaattctttt cgtctcttat atgagcggag gattcaaacc ggtatccgaa tataaaaaac
     1981 tgggatcggt acagtttata tttcaatata ttacctattt cttcaaatca atcttggtca
     2041 tgttggcaat cgctttggga caaaaagccg gagatcttta ttttcaaaaa gaccaaattc
     2101 catggggcgg ttattttttg gcggtttctt ggggattcat tcaatttatt attcaaaatc
     2161 ccgcaaccgg gctgttcact gtggcattat ctgtcttatt tggggttctg ttcatgatta
     2221 atcataaaaa cgcgcgaatt gtttttccga tgattgccat tgttctgatg ctctaaaaga
     2281 gtgatcatta agaaaaagaa agggttgaag agatcttccg atgggaaaaa tgattttaat
     2341 tggtgtcgta atggtgatat tatgtattgt tgccgggaaa attataaaaa gaacgatgta
     2401 aattgacaac gtttttgttt ccgattctat gaagcttcca ttcacatcac aatgccgatc
     2461 aaacattgtg agaatattca ttaataatcg caaaaagcat ttacaagata aaaaggaaaa
     2521 agtataattt gaaaatagcc gtagccaaga ggtatggctt tttatttttg tctgttcaat
     2581 ctataaaatc tcttagtgaa aggggattca tatgaaaaaa tcagctctgc ttgcaattgt
     2641 agttttgtgt ctggctgttt caacaggcgt gcttggcttc atgtattcaa ccaaaggaga
     2701 taaaactgcg gaagaagcgg ctccgatggt acaaatcggg gtgatgacga ataagtgggc
     2761 ggcatccgct cacggagatt catcttccga agcattcaga cattgggata aagaaggtga
     2821 agtacccgtt acctgtgcta aatgccattc gtctgcggga atgcaggatt atctcggagc
     2881 cgatggaaca gccgccatga cggtggataa ccctgccaaa gtgggatcgg ttgtagattg
     2941 ttccgcatgt cacaatgaag tgatccagaa cacaacttcg gtggtctttc cttccggaaa
     3001 agaagtcgca gccgaaccgt cttatgcgtc ctgctggaca tgccatcagg gcacggaagc
     3061 cggaattaac ggatcgctag atcagaaaat cggttcgacc gctccggatg ataagggcga
     3121 aaatctgggc ttcgtcaatc cgcattattt gaatgtggcg ggtatttatc tgggatccga
     3181 agcaaacgcc ggttatcagt atgaaggaaa gacatatgcg gctcgctttg agcatgcttc
     3241 gggtgtacag acctgtaccg aatgccatga tcctcacagc ctgcatttgg cagatccggg
     3301 attggaaaaa tgctcaacct gccatgcgga agtcgaaacc tggcccgatc agcaaaaaat
     3361 tcgccgcacc aaagaagacc tggacggtga cggaaatacc gacgaatcca cctatgagga
     3421 aatcaacgga ttactcgagc gggagcttca agcgatggtg acctattcgg ttgatgttgc
     3481 caaagttcag atcggtaatt ccgatgtcta tccctactgg tttgcggata aaaacggaaa
     3541 cgggactcag gaagaggatg agaaacagta tgccgactgg acaccgcgtt tgatgtatgc
     3601 cgcatataac tttaaactgg tcaagacctc agccggatat atccataatc cgacttattc
     3661 cgcacaattg ttaatcgatt cgatcagtga tttggctgcg ggtgattcgg caatttcggt
     3721 tgacggtatc gagagaccgt aggaagaaag gtaatcaaac atgaatactg ctgccagtcg
     3781 ttcggcaaga agagcttata tcgctcaact gcgagttaaa aggatattct ggatttgcct
     3841 gtttgttgtc tttgccttgt tcccgttggt tgtcgctgcg atcaataaca cgacaaacgg
     3901 gacgtggcgg gcggtgccgc gtgagatcgg ttcttttgcc gggatgttag ggttgtccct
     3961 gatgatcctt tcctttattc cgattatgcg agaaaagaaa attaccagcg cttttaatct
     4021 ggatgtggta tataaatttc atcattcatg ctccatcctt gggttttggc tcgtggtttt
     4081 tcatcttgtg gctttgtgga tcaacaatca ggaaatcgga cgactggtaa atatatttgc
     4141 agattatccg ctttatatga aagccggccc cattgcttta ttggccgccg gctgtttggt
     4201 ctatttttct tatttccgta aggaattgaa gcttaactat gattattgga aaatcgggca
     4261 ttcccttctt gcgatactta tggtggtttt tggcttaatt cacacttttg gggtgaatta
     4321 ttacagcggc aatgcactaa tacacggtta ctatgttttc ttgaccgtct tggctgcgat
     4381 tgctattctg tggctgcgcg ttttctcttc actgtctgcc atgtgcaaac cttatgtcat
     4441 tagggaagtg ctgattcgca acaacgcaac caccgaatta agactggaat tcgccggaaa
     4501 aggaaatcaa aaaccgattc gttatcatgc cggacaaatc gcatggataa cggtgcgaca
     4561 ttcccgcttt tctttcaaag aacatccgtt ctcgatcgct tcctcggata tcgaccaaaa
     4621 taaaatcggc tttgccatcc gcgagttagg cgattttacc gctacgatta aggatctgaa
     4681 acccggtgag atcgtttatg tcgagggcgg ttttggcatc tttaatccat atatattggg
     4741 cgatgaaggt tttgtgttga tcgccggcgg aatcggtatc gctccggtta tgggaatttt
     4801 gcggtcgatg gcagatcgaa aagatcggcg gcatgtggtc ctttattatg ggagtcgtga
     4861 caaagaaagc atcggatttt atgacgaact gaatgaactg tccaaaaaat taaatttgga
     4921 agttgtccat gtcctcgaga agaccgatga tccgtccttt gaaaaaggtt atgtgaccaa
     4981 agatgtgatc gaacgacatt acccgaagaa taaggatcga tacgacgtgt ttatctgcgg
     5041 acccggcccg atggttcaga ttgtccttcc ggctttaaag aagatcggaa ttccgaaaga
     5101 gaatatttgg ccagaattct atgatatggc ttaattgaaa tttttggaaa tgatttaaaa
     5161 gggctgtctg tcgatttcgt cgggcagcct ttttattacg aaagtataat acctaaacaa
     5221 ggtatttttg ccgataaaac gatcgatact ttaggaaaga aaatggatca atacgatatt
     5281 attatcat
//
LOCUS       DAOMIV010000083         5270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_15867_length_5270_cov_3.736337, whole genome shotgun sequence.
ACCESSION   DAOMIV010000083 DAOMIV010000000
VERSION     DAOMIV010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15867_length_5270_cov_3.736337"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PKV59_03070"
     CDS             <1..763
                     /locus_tag="PKV59_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP45766.1"
                     /translation="TVITTNCRLQDLDGRIASRLEDPSLVTRIVIQAPDYRKPMANTV
                     ESDDLSALHRLSNRTFENFDSRVGENLSEEASAQLTNAFNLAYNFAQHPTGWLVFSGP
                     NGVGKTHLAAAIGNYRQHQMDHPLFVAVADLLDHLRATFSPVSTTSYDIAFEKIRNAP
                     LLILDHLDTANATPWAKEKLFQILDHRYLTPLPTVITTVLPIQDIDPNIRSRLVDFQV
                     CQIAQMFQVPMYSRNPQVSILPERTRKGYPSRRGK"
     regulatory      775..902
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1058..1996
                     /locus_tag="PKV59_03075"
     CDS             1058..1996
                     /locus_tag="PKV59_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HQP45767.1"
                     /translation="MRKKTLFYCVVFLTILFSIAMPVSAVRDTDVAEKITVTDDLDRK
                     ITLELPLESVVSLAPSVTEIVCALDLCDELIGVDVASNYPDEVTKKAIVTNYDMSINK
                     ELILSLDPELVIISELTAVDQVKQLEDMGLTVYYLKNPKTLDDLDSYFMKVGEVTGHE
                     EQAEELIAEMKNRIAVVDETIAGATTHPLVFYELDATDVSKPWTASSGTFIDELITRA
                     GGKNVAADLKGEWVQISLEALIRSNPEIIILGDSNYGITPESVIERPGWDVLSAVKNK
                     KIYPINSDIGSRPSPRLVDALEILAGYIHPELFEKK"
     gene            2068..3045
                     /locus_tag="PKV59_03080"
     CDS             2068..3045
                     /locus_tag="PKV59_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQP45768.1"
                     /translation="MSIAGIWVGSVPLGISQVWDILKYRITGGALPAELAGTDRILFV
                     LRIPRTIMMLLTGAALACSGASYQGLFRNPLADPYLIGVSTGASLGAVIAMSVQWPYT
                     KWGTMMTPILAFVVSLLTVWVVFKLGKVSGITGNTGLILSGVAVNSFITAVTSVWMLF
                     SHRELRTSFSWMLGGMTVNGWEPIKIVIPFILIGIIGQFLLSHRLNVLQFGEEQAANL
                     GISVHKTRIGIVFFATLTTAAAISFSGVIGFVGLIIPHVIRILTGGDYRKLIPLSALG
                     GAFFLLLADVIARVIIAPQELPVGIITAIAGAPFFLWLLNRNTRREIER"
     gene            3042..3809
                     /locus_tag="PKV59_03085"
     CDS             3042..3809
                     /locus_tag="PKV59_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45769.1"
                     /translation="MISLSDVSVHYGDQPVVQNISFDIRQGKVMVLIGPNGSGKTTII
                     RGITGSVKLSGGQIKYDGKNSADLSETERAKYLSVVPQNSLLPDGFSVYETVAMGRTP
                     YLGLLGKLSEEDRWRVDEAIRETAIFDLRKKDVRSLSGGEQQRVILARALAQDTPVMI
                     LDEPTTHLDLNHTIGILSLVRNLSREKGTAVLIVLHDLNLAARFADEIIILKQGNILA
                     MGTPEEVLTENNLTAVYHVPMIVFRHDGISTPIVLPK"
     gene            3879..4526
                     /locus_tag="PKV59_03090"
     CDS             3879..4526
                     /locus_tag="PKV59_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HQP45770.1"
                     /translation="MNYKAVFFDLDETLYPMGNESIKVITDRMNEYIRRSLAIPDSEV
                     KNFREDLYKTYGTTAKGLVKNYEVDLYDFMHYVHNVDISPYIQPAPEVRALIEKIQIP
                     KFIFTNSDRFHAIRVLKLLDNYQCFDGIIDFLDVYPYCKPEQEAFEKALAMTGIDSGR
                     GCIFLDDNPTNISTAHQLGLYAIQVGSRARSEEADAFLSRIEDLPQLEIYSEIIT"
     gene            4523..4804
                     /locus_tag="PKV59_03095"
     CDS             4523..4804
                     /locus_tag="PKV59_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45771.1"
                     /translation="MTTKGWIILIALIVVVMGINILLIVYREKIKAYFEKRRKPASKS
                     NQDDSFSKLTKKVKEVKKGDFLFPESEHYKRLRQEMDRLENLERDKDQK"
     gene            4801..>5270
                     /locus_tag="PKV59_03100"
     CDS             4801..>5270
                     /locus_tag="PKV59_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45772.1"
                     /translation="MNQRIIRIIAIVIILISCFVSGFSAGYIIKMLPETVDKINISSE
                     SSLLPKLLSVTEQGTGMKRQQNTDELFKPFWETWDLVHQYYVDQPVDDNSLMEGAIRG
                     MLESLGDPHTRYSDPESYKQESQYIAGEEYEGIGAWGDVSGDYVEITSPMKGSPA"
BASE COUNT         1378 a         1121 c         1295 g         1476 t
ORIGIN      
        1 gacggttatc accaccaatt gccgcttgca ggacttagac ggtcggattg cttcgcggtt
       61 ggaagatccg tccttggtta cccggatcgt gattcaagcg cccgattatc gaaaaccgat
      121 ggcaaatacg gtggaatcgg acgatttgtc ggcacttcat cgtttatcca accggacgtt
      181 tgaaaatttt gattcgcgag tcggagaaaa tctgtccgaa gaagcctccg ctcagttgac
      241 caacgccttc aatttggcat ataactttgc gcagcatccc accggttggc tggtattttc
      301 cggcccgaac ggcgtcggta agactcattt agcggctgcg atcggaaatt accggcaaca
      361 tcagatggat catcccctgt ttgtggcggt ggccgatttg ctggatcatc tgcgggcgac
      421 attcagtccg gtcagtacca cttcctatga catcgctttc gaaaaaattc ggaatgcgcc
      481 gcttttgatt ttggatcatc tcgatacggc aaatgctacc ccgtgggcga aagaaaagct
      541 gttccaaatt ttagaccatc gttatttaac gccgctgccc accgtgatta ccaccgtttt
      601 acccatccaa gatattgacc ctaatattcg cagtcgtttg gttgactttc aggtttgtca
      661 gatcgcgcaa atgtttcagg ttcccatgta cagtcgtaat ccgcaagtct ccattttgcc
      721 ggaacggaca aggaagggat atccttcacg acgcggcaaa taaccataaa gaatcgaaga
      781 ataaatttgg tataattcaa aagccagtgg aaaacaagga aatcggttcg aaaccgatgc
      841 tgacccgcaa ctgtaagaag atcatcttcc aagccagacc tttgttttcg acaaaaatcg
      901 ttcctcgggg attgggaagg catgccgatt agaccccgtc aggggttttc ttttttgaat
      961 atcctcataa cgaacttccc gctctccgat tgaccccgga aaggttcgtt ttttcgcccg
     1021 gccccggaac gagaaaatct atataacgag gtgaattatg cggaaaaaga ctttattcta
     1081 ttgtgttgtt tttcttacga tacttttttc aattgccatg ccggtttcgg ccgtccgcga
     1141 taccgatgtc gctgaaaaaa tcacggtgac ggatgacctt gatcggaaaa ttacgcttga
     1201 acttcccttg gaatccgtcg tttctttggc gccgtcggta accgaaatcg tatgcgcatt
     1261 ggatttatgt gatgaattga tcggagtcga tgttgcttcc aattatccgg atgaagtgac
     1321 gaagaaagcg atcgtcacaa attatgatat gtccattaac aaagagctta ttctttcact
     1381 cgatcccgaa ttggtgatca tttcggaact gacggcggtt gatcaggtaa aacaattgga
     1441 agatatgggc ttgaccgtct attatcttaa aaatcccaag acactggatg acttagattc
     1501 ctattttatg aaagtcggag aagtaaccgg tcatgaggag caggcggaag aactgatcgc
     1561 agagatgaaa aacaggattg ccgtcgtcga tgaaacgata gccggggcaa ccacacatcc
     1621 gctcgtgttt tatgaactgg atgcgaccga cgtttccaaa ccctggacgg cgagcagcgg
     1681 taccttcatt gatgagctga ttacgcgggc cggcggaaag aatgtcgcag ccgatctcaa
     1741 aggagaatgg gttcagatta gtttggaagc gttgattcga tccaatccgg agatcattat
     1801 tctgggtgac agcaactacg ggataactcc ggaaagcgtc atcgaacgac cgggctggga
     1861 tgtcctatcc gcagtgaaaa ataaaaagat ctaccccatt aattccgata tcggatcacg
     1921 cccgagtccg cgtctggtcg atgctctgga aattttagcc ggatatattc atccggaact
     1981 gttcgaaaag aaataagcag aggcacctat tctatgcgca gatcggcatc ccggttaggc
     2041 atttttttgc tcatcggaat attcgttatg agtattgccg gtatatgggt cggtagtgtc
     2101 ccgctcggca tttctcaggt atgggatata ttgaaatatc ggataaccgg aggtgctttg
     2161 cctgccgaac ttgccgggac ggaccggatt ttatttgtct tacgaattcc gcgaacgatt
     2221 atgatgctgt taaccggtgc cgctctggct tgcagcggcg cttcctatca gggattattt
     2281 cggaatccgc tggcggaccc ctatttgatc ggcgtctcaa ccggtgccag tttgggggct
     2341 gtgattgcta tgtctgtaca gtggccttat acgaaatggg ggaccatgat gacgccgatt
     2401 ttagcctttg tggtatcgtt gctgacggta tgggtcgtct ttaaattggg caaggtcagc
     2461 ggaataaccg gcaataccgg attgattctt tccggcgtgg ctgtaaattc ttttattacc
     2521 gccgtaactt ccgtttggat gttgttttct caccgagaat tgcgaacatc gttttcatgg
     2581 atgctgggag gaatgaccgt taacggttgg gaaccgatta agattgtgat tccttttatt
     2641 ctgataggaa taatcggtca gtttttattg tcacatcgtt tgaatgtcct tcaattcggt
     2701 gaagaacaag ccgcaaatct gggaatctcg gttcataaaa cccgaatcgg gattgttttt
     2761 ttcgcgactc tgaccacggc ggccgcaatc tctttttcgg gagtaatcgg attcgtcggc
     2821 ttaatcattc ctcatgtcat ccgtattttg acgggcggag attatcggaa attgattcct
     2881 ttatcggcat tgggcggtgc cttttttctg ttactggctg atgtgattgc gcgggtgatt
     2941 attgcaccgc aggaattacc ggtagggatt atcaccgcca tagccggtgc gccctttttc
     3001 ctttggctgt taaatcgaaa taccaggagg gaaatcgaac gatgatctct ctttccgatg
     3061 tatccgttca ttacggcgat caaccggttg tccaaaatat ttcgttcgat attcggcaag
     3121 ggaaagttat ggtattaatc ggtccgaacg gttcggggaa aacgacgatt attcgcggta
     3181 ttactggcag tgttaaactt tccggcggcc aaatcaagta tgacggaaaa aactcggctg
     3241 atttgagtga aacggaacgt gccaaatacc tgtcggttgt tccgcagaat tccttgctgc
     3301 ccgatggatt ttcggtttac gagaccgtag cgatggggag gacgccgtat ctcggtttat
     3361 tggggaaatt atccgaagag gatcgatggc gagtggatga ggcgattcgt gaaaccgcca
     3421 tttttgacct tcggaaaaaa gatgttcgtt ctctttccgg cggagagcaa caacgggtaa
     3481 ttttagccag agcgttggca caggatacgc cggtgatgat tttggatgaa ccgaccacac
     3541 acctggactt gaaccacacg atcgggattc tttcattggt ccgaaatttg agccgcgaaa
     3601 agggaaccgc cgtgttgatc gttttacatg acctcaattt ggctgccaga tttgcggatg
     3661 aaatcattat tttgaaacag ggaaatatct tggcaatggg aacgccggag gaagttttaa
     3721 ccgaaaacaa cctgaccgct gtctatcatg ttccgatgat tgtctttcgt catgacggta
     3781 tttccactcc gattgtcctg ccgaaatagc ggtttatttc gtcagaattt ccgatatatt
     3841 ccatttaatc gatctccgat tcggttatac ttttttgcat gaattacaaa gctgtttttt
     3901 ttgatttaga tgaaactctg tacccgatgg ggaatgaatc gattaaagtg attacggatc
     3961 ggatgaatga atatatccgg cgttctttgg caatccccga cagcgaagtg aagaattttc
     4021 gcgaagacct gtataaaact tatggaacga ccgccaaagg gttggtaaaa aactacgaag
     4081 tggatttgta tgattttatg cattatgtgc ataatgtcga tatttcgccg tatattcagc
     4141 cggctcccga agttcgggca ctgattgaaa aaattcaaat tccgaagttt atttttacca
     4201 attccgatcg ttttcatgcg attcgtgtat tgaaactctt ggataattat caatgttttg
     4261 acggaattat tgatttcctg gatgtttatc cgtattgtaa accggaacag gaagcctttg
     4321 agaaagcgct cgccatgacc ggaatcgaca gcggccgggg atgtatcttt ctggatgata
     4381 atccgaccaa tatcagcacg gctcatcaat tgggccttta cgcgattcaa gtcggatcgc
     4441 gagcaagatc ggaagaagcc gatgcgtttc tttcgagaat agaggatctt cctcaattgg
     4501 aaatctattc ggaaataata acatgactac aaaaggttgg atcattttaa ttgccttaat
     4561 tgtcgttgtc atggggatta acatattgtt aattgtctac cgtgaaaaga tcaaagctta
     4621 ttttgaaaaa agacggaaac cggcctccaa aagcaatcaa gatgattcct tttcaaaact
     4681 gacaaagaag gttaaagaag taaaaaaagg tgatttcctg tttccggaat ccgaacatta
     4741 caaacggttg cgtcaggaaa tggacagatt agagaatctg gaaagagata aggaccaaaa
     4801 atgaatcaac gaatcattcg aatcattgcg attgtcatta ttttaatcag ttgttttgta
     4861 tccggtttca gcgccggata tatcataaaa atgttaccgg aaaccgtcga caaaatcaat
     4921 atctcatccg aatcgtcctt gcttccgaaa ttattgtcgg tcacggagca aggtacgggg
     4981 atgaaacgcc aacaaaacac ggatgaatta tttaagccgt tttgggaaac ttgggatttg
     5041 gttcatcagt attatgtcga tcaaccggtc gatgataaca gcctgatgga gggcgcgatc
     5101 cgcggaatgc tggaatcttt gggcgatccg catacccgct attccgatcc cgaaagctat
     5161 aaacaggaat cgcaatatat tgccggcgaa gaatatgaag ggatcggcgc atggggtgat
     5221 gtgtccggtg attatgtcga aatcacttct ccgatgaaag gtagtccggc
//
LOCUS       DAOMIV010000084         5221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_16094_length_5221_cov_4.331978, whole genome shotgun sequence.
ACCESSION   DAOMIV010000084 DAOMIV010000000
VERSION     DAOMIV010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16094_length_5221_cov_4.331978"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKV59_03105"
     CDS             <1..614
                     /locus_tag="PKV59_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HQP45773.1"
                     /translation="QNHNVSVIESRPEVLQYVHKELPTDAIFEGEILDPDTLEQAGIK
                     DANVLVASTDDDKVNLLLCYIAKERYNVPRTIARVNNSKHAWLFDEKFHVDYALDQAT
                     IFSMMIEEEISAGHMMVLLQLGKGDFSLVKEVIPADSSVIGKKMMDLNLESVIAAIIR
                     NGNVIPPHGDTVFMEKDEVIALTDREGLNQLRNLFSSQSIDRK"
     gene            854..1834
                     /locus_tag="PKV59_03110"
     CDS             854..1834
                     /locus_tag="PKV59_03110"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45774.1"
                     /translation="MKKISVSVLLFIMIMSVLIVHPVTAEDDIIEIGVLQLVEHASLD
                     AAYRGFVDALKEAGYEDGVNIKIDYQNAQGDQANLMTISERFVKNQVDLILAIATPAA
                     QSVASATTDIPILFTAVTDPVAARLVESNEAPGLNVTGTTDAGPIDKQLELLKELYPD
                     LKTLGIIYNSSEVNSEVQAKQAEEIAKNLGWEVKLGTITSVNDIEQVATSLAGKVEAF
                     YAPTDNTIASAMPNLAKVAEEHSLPIIAGAPEMVEAGGLATVGIDYYKLGAQTGKMAV
                     RILKGEAKPAAMPVESLKDVDVVLNKDYAKAIGYSFPQSVLDKATKIIGE"
     gene            2115..3092
                     /locus_tag="PKV59_03115"
     CDS             2115..3092
                     /locus_tag="PKV59_03115"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45775.1"
                     /translation="MKKISALILFVLVVTALPLGIVSAEDAPIKIGVLQLVEHAALDA
                     AYQGFVDALKEAGFEDGVNIEIDYQNAQADQSNLMTISERFVKNNVDLILAIATPAAQ
                     AVASATTTIPILFTAVTDPVAARLVESNEKPGLNVTGTTDAGPIDKQFALMKELFPDL
                     KTIGIIYNSSEVNSEVQAKQAEEIAKEMGWEVKFGTITSVNDIEQVANSLAEKVDGFY
                     APTDNTIASAMPNLVKVAEEKKLPILGSEPGMVEGGALMTVGIDYFKLGQQTGKMAVK
                     ILKGEAVPAEMPVESLKDVDIVLNQATAKAIGFEFPESVLKKATKVIGE"
     gene            complement(3184..4116)
                     /locus_tag="PKV59_03120"
     CDS             complement(3184..4116)
                     /locus_tag="PKV59_03120"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HQP45776.1"
                     /translation="MFELLFLGTSASAPSIKRNLPSLLVIHDEFRFLIDCGEGTQRQI
                     LTAGCGFKRLNKILFTHSHLDHILGTAGLLSTLLRWESMEDMDIYGGEDTIARVHDLL
                     FSVVLRGNRAPDFLRFHLLSEGILFDEKDFQVSCFPVNHRGSDSFGFRFDKKSRRPFL
                     PEKAEALGIPPGPWRRDLANEERVVLPDGRTIDPDQVLGDLIPGESLVVVGDTGETDS
                     LLPYVQHADALVIEATYTEEEADMAFQYSHLTAKHSARFAKTAGVGTLYLTHISRRYR
                     EKDILEEAQAIFPNTVVARDLAHYQIKKQAQTVK"
     gene            complement(4373..>5221)
                     /locus_tag="PKV59_03125"
     CDS             complement(4373..>5221)
                     /locus_tag="PKV59_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQP45777.1"
                     /translation="LLFLIFSNILNVGLLYFFIVILGTGIKGAAYGTVISETSAGILT
                     FIYLLRSQEKYGISIKGLKPDWQIGKQMINIGLPAGLQTSIFSVGFLLQQNLINSFGP
                     TVIAAYAVVTRIDQFAMLPMNSFAVGITAFVGQNTGARKMNRTIEGIKQTLILSQSLT
                     IALVSILFFFGANVMALFTKDLAVVQSGKLILRTLSAGYILVNTYTVLSGAVRGVGDT
                     VAPLLASFICNVIIRVSLAYFLVKMTGNYQLIFVSISISWTLASAFLWIYYKKGFWQK
                     KIRGII"
BASE COUNT         1505 a         1093 c         1255 g         1368 t
ORIGIN      
        1 aacaaaatca caacgtatcc gtaatcgaat cacgcccgga agttttgcaa tatgttcata
       61 aggaattgcc caccgatgcc atttttgaag gggaaatatt agatccggac acgcttgaac
      121 aagccggaat caaggatgcg aatgttttgg tggcaagtac ggatgatgat aaagtcaatt
      181 tgttgctttg ctacatcgct aaggaacgtt ataacgttcc tcggaccatt gcgcgggtaa
      241 ataactccaa acatgcctgg ctgttcgacg aaaaatttca tgtcgattat gccttggatc
      301 aggcgaccat tttctccatg atgatagagg aggaaatttc ggccggtcat atgatggtac
      361 tgcttcaact cggaaaaggc gatttttcat tggtcaagga agttattccg gctgactcgt
      421 cggtgatcgg caagaaaatg atggacttga atttggaaag cgtgatcgct gccattattc
      481 gaaacggaaa tgtgattccc cctcacggtg atacggtttt tatggagaag gatgaagtaa
      541 ttgccttgac cgatcgggaa ggtttgaatc aattacgcaa tttgttcagt tcacaatcga
      601 tcgacaggaa ataagcgatt gctatggtca tgtcattaag catgtcatga ttttcaaaat
      661 aagggttgaa aacgaaaacg gaatcatgtt ataaaatggg tatgcaaaat attcactcgt
      721 ttttcttctt tttctttttt gccacacctc acaaagttgt gcagcgtgaa ttttttgcat
      781 aaatcaatcc ggtagcaaaa agacctcctg cacaacagga ggtcttttta ttttatggtt
      841 caggagtcat acaatgaaaa aaatatctgt gtcggttctt ttattcatca tgattatgtc
      901 tgttttgatc gttcatcccg taacggctga agatgacata atcgaaatcg gtgtgttgca
      961 attggttgaa cacgcatcac ttgatgcggc ttaccgggga tttgttgatg ctctcaagga
     1021 agccggttat gaggatgggg tcaacatcaa aatcgattat caaaatgctc agggggatca
     1081 agccaatctg atgacgatta gcgaacggtt tgtgaaaaat caggtggatt tgattttagc
     1141 cattgccacc cccgccgcgc agtccgttgc ttccgcgact acggacattc cgattctgtt
     1201 caccgctgtc acggatccgg ttgcggctcg gttagtggaa agcaatgaag ctcccggtct
     1261 gaacgtcacc ggcacaaccg atgccggccc gatcgataaa cagcttgagc tgttgaagga
     1321 actttatccc gatttgaaaa ctttggggat catctataat tcctccgagg tgaattcgga
     1381 agtacaggca aaacaagcgg aagaaattgc gaaaaatctc ggttgggaag tcaaactcgg
     1441 tacgatcact tcagtgaatg atatcgaaca ggtggcaacc tctctcgcgg gtaaggtaga
     1501 agctttctat gcaccgacag ataacacgat cgcttctgcc atgccaaatc tggcaaaagt
     1561 tgcggaagaa cacagtttgc cgatcattgc gggtgctccg gagatggtcg aagcgggcgg
     1621 tttggccacc gtcgggatcg attattacaa attgggtgca caaaccggaa agatggctgt
     1681 ccgaatccta aaaggcgaag ccaaaccggc tgccatgccg gttgagtctc tcaaagatgt
     1741 ggacgttgtt ttgaataagg attatgccaa agcgatcgga tacagcttcc cgcaaagcgt
     1801 gttggataag gctaccaaga tcatcggaga ataagaatga gagatgattt gagatgatga
     1861 ttaccgattt cggcaatcat catctcaaaa aatggatgtc atcaatttgt taaaatcatc
     1921 ttgcataaaa atcgaaaaat gtgctataaa aaagccatgc aaaaaacgaa tcgttcctat
     1981 tatttttacg gttattatta cggaaaatca ccccattcgg tgtggcgtga agtttttgca
     2041 taaatagaat cgtaagcaaa agacctcctg cacaccggga ggtctttttt tattctataa
     2101 ccaggagaaa gatcatgaaa aaaatatcgg ctctcatttt gtttgttctt gtagttacgg
     2161 ctttgccgtt ggggattgtt tcggctgaag atgctccgat taagatcggt gtattgcagt
     2221 tggtcgaaca tgcggctttg gatgcggctt atcaaggatt tgtggatgcc ctcaaagaag
     2281 ccggctttga agacggggtg aatatcgaaa tcgactatca gaatgcacag gcggatcaat
     2341 cgaacctgat gacaatcagt gaacgtttcg ttaaaaacaa cgtggatttg atcctggcta
     2401 tcgctacacc ggctgctcag gcggtggcct ccgcgacaac aacgattccg attcttttta
     2461 cggcggtaac cgatccggta gcagctcgct tggtcgaatc gaacgaaaaa ccggggttga
     2521 atgtgacggg aaccaccgat gcggggccga ttgacaaaca gtttgccctg atgaaagagt
     2581 tattcccgga tttgaagacg atcggcatta tttataattc ttccgaagtc aattccgaag
     2641 tccaagccaa acaggctgaa gaaattgcca aagaaatggg ttgggaagta aagttcggaa
     2701 caatcacttc ggtgaatgac attgaacagg ttgccaactc tctcgcagaa aaggtagacg
     2761 gattttatgc gccgacggat aatacgattg cctcggcgat gccgaatctt gtaaaagtgg
     2821 ctgaagagaa aaagctgccc attttgggaa gcgaacccgg gatggttgaa gggggcgcat
     2881 taatgaccgt cggaatcgat tattttaaac tgggtcaaca aacgggtaag atggctgtaa
     2941 agattttgaa aggggaagcg gttcccgcag agatgcccgt tgagtcgttg aaagatgttg
     3001 acattgtgct gaatcaagcc acagccaaag caatcggttt cgaatttccg gaaagcgttc
     3061 tcaaaaaagc caccaaagtc ataggcgaat gagaacatct cttcagaaag aatccccgaa
     3121 aaagcgggga ttctttttta acgaacagac tctcagacgt tattttgtgc cgagacgtgg
     3181 aaattacttt acggtttggg cttgcttttt tatttgatag tgcgccaagt cacgggcgac
     3241 aacggtattg ggaaagatcg cttgtgcttc ttccaaaata tctttttcgc ggtatcgtct
     3301 ggaaatgtgg gttaaatata acgttcccac accggctgtt ttcgcaaacc gggcagagtg
     3361 tttggcggtc agatgagaat actggaatgc catgtctgct tcttcttccg tataggtggc
     3421 ttcgatgacc agcgcatccg catgttgaac gtaaggcaat aacgaatcgg tttctcccgt
     3481 atcaccgacg acgaccagac tttctccggg aattaaatca cccagtacct gatccggatc
     3541 aatcgttcgt ccatccggta ggaccacgcg ttcttcattt gccaaatcac gacgccaagg
     3601 gcccggcggg attcccaatg cttccgcttt ttcgggaaga aacggccggc gcgatttttt
     3661 atcgaagcga aagccgaacg agtccgaacc acgatgattt accggaaaac aggatacttg
     3721 gaaatctttt tcatcgaaaa gaatcccttc ggaaagcaga tgaaatcgca aaaaatcggg
     3781 agcacgattc ccgcgcagaa caacggaaaa aagcaggtca tggacgcggg cgattgtatc
     3841 ttctccgccg taaatatcca tgtcttccat gctttcccag cgaaggagcg ttgaaaggag
     3901 tccggctgtt cccaaaatat gatccagatg actgtgagta aaaagaattt tattcaatct
     3961 tttgaaaccg catccggcgg tgaggatttg tcgttgagtg ccttctccgc aatcgatcaa
     4021 aaaacgaaat tcgtcatgaa ttaccaataa agacggtaag tttcgtttaa tggagggcgc
     4081 ggaagccgaa gttcctaaga aaagtaattc aaacaaattg gtttatgttt tggcctcaat
     4141 ttctgtcaga tcgaagttat tttaccagac agtgggcaaa gttctcgacg ttttcgggcg
     4201 gatatcatac gaagcagatt tgataccggc agatttcgct gaattaccga tgcggaggtc
     4261 atgtacgctc atgaaattga cttgactttt attttccgat acgctttaat taaaggtggc
     4321 ggacagctta tgggtgaccc cctcatccat aatgtctgat ccgccatgtt ttttaaataa
     4381 tcccgcgtat tttcttctgc caaaaacctt ttttataata aatccaaaga aaagcgcttg
     4441 ctaaagtcca ggagattgaa atacttacaa aaatcagctg ataatttccg gtcatcttga
     4501 caagaaagta agcaagggaa acgcggataa tcacattaca gataaaactt gccaacagcg
     4561 gtgcgaccgt atctccgacg ccgcgaacgg ctccgcttaa gactgtatag gtgttgacta
     4621 agatatagcc ggccgaaagc gtccgcaaaa tcaattttcc gctttggacg accgcaaggt
     4681 cttttgtaaa aagcgccatg acatttgcgc cgaagaagaa aagaatggaa accaaagcga
     4741 tggtcaacga ttggctgaga attaatgtct gtttaattcc ctcaatcgta cgattcatct
     4801 ttctggcacc tgtgttttgc ccgacaaaag cggtaattcc gaccgcaaat gagttcatcg
     4861 gtaacatggc aaattgatcg atccgtgtca caaccgcata ggcggcgatg accgtcggac
     4921 cgaaactatt aatcaggttt tgttggagca aaaaaccgac ggagaaaatg gaagtttgca
     4981 accccgctgg taaaccgata ttaatcattt gtttcccgat ttgccagtcc ggtttgagcc
     5041 ccttaatgga aatgccgtat ttttcttggg atcgcagcaa ataaataaat gttaatattc
     5101 cggcagatgt ttcggatatt accgttccgt aagccgcgcc cttgataccg gttccgagga
     5161 tgacgataaa gaaatataac aacccgacat tcaggatatt ggaaaaaatc agaaagagca
     5221 a
//
LOCUS       DAOMIV010000085         5217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_16118_length_5217_cov_4.312670, whole genome shotgun sequence.
ACCESSION   DAOMIV010000085 DAOMIV010000000
VERSION     DAOMIV010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16118_length_5217_cov_4.312670"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5217
                     /locus_tag="PKV59_03130"
     CDS             <1..>5217
                     /locus_tag="PKV59_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45778.1"
                     /translation="LVIGENRQELNSDQYHGSQTVKIQEDTPVQLISESEMGKEVKNL
                     VLKAENPQMELVKFIIWIRPSAPKTNTSPVQRIDSSDGSSDEFTAKYAELVPDPSSLT
                     GYKIVRYEFDRQIISGEQIMLEWEVKDAETVLLEPLSDQFVSQKGAQFYFPTQSTNFT
                     LSVSDGSRLKSYTLPISIRTEKNVEAVAPKIDLFDANPVTLTQPGKTTLSWSVSGDWT
                     RIQLIASGASFDSIHADTNNENAIPLTISQNSGSILADYLAPTGFLSLNLKENASFIL
                     KAWNGNLSNSSIVNVRVNPSEPPAVLKSPDLKITAIFTDSGNYMVGDTITVSTAFDGL
                     SSIDSIPSGTILISDGLSNCVITLPKNSCDLNLRVAGNRTITAQYSGNASYRSAQTSQ
                     NINVSTENKILLDIRPSLFPEKSIYTVGEEITITAGLPDFLSDQPAATGSLFITDGIS
                     NCLLTLPKNNCELTIRAAGNQMITVNYSGDEDYYPAQAKISIRAENQIPSATPSPQPT
                     LTPIITPTIEPKNLSLKIAGVYPQKSSYHIGDQIDIYIDPDYDALRSDFPKEKIRISD
                     GYATCLFSVESQNHCSLQLIDPTTTKITAAFPGDGFYLPVTSEPWIIKVEEPPKAKTT
                     TSILNLNPVKDKYQIGDIVRVYAATEPDQSSEIPITGTITISDSYSSCAILLPDQKFC
                     DLTLSNGLAKEITAIFSGDDIYASSVSSVTPIQISMVEVSAISVQAMQYSDCDHFMPD
                     PNHVIPLKTDTSVVPEEHYFNLDEQFPIGSGFYINAIVRSKVGHLDTSPGTFNAKICS
                     KEEPDSCVISDTVEAVRNQDDFTQAMGRLEIPPIYRTGKHLLTITFTSDPELYGTETA
                     TFSLWNVQRGQLVLTPHSVPEPDPTYENYLWNNLTATEKNSTDYLFDAYLLKGNNLRC
                     PVALQSSLYEKPFGVTLQVEVDPVLPGATITDSEWKAAILSNGYEAAMVDKLQPNSML
                     WDRNTCRWETNNENWFVHCSDVGISEPSTIRYALDYSDPNYIIPNRQNPADDFSAATI
                     RVAIGKKLAFIHSKELLGALTPNTPYWINYEGSSFSWFASSTACDQEPYRGDYVSNSS
                     IPADKIIHIHLLPLSRSALDSNFKWERIPAEVKIDYTPAENPFAVLSTYHGICGNCSG
                     KPLKNFDYIPNLGCVQTVAGQINLSPEEGTLLAGTACPDGQPKMTGIQSTFCQLEFHT
                     ASEVHFSFSGNDDYQPADRQVALNISGISASGSEQSVRLMSAPLMPNPSSEPQPVTIL
                     PTFTDSLGNVVYEHYVGEKYILRLQSSVPLNENIMLIADLPDIFSTDSLDPDQSSCLK
                     SIDFEEHQLRIAGSNFTGENNTFDCEIVFKQPVSPGTIQTISFILENMNEKTNYRMTP
                     FSWSGIPQSVVKKEVSIEATVFNSNETICDNLSPFCGVMYPSESYQLKLKIALPNPPA
                     ENQKKVIFHIRWPEGTTIRNENSVPNSNCEIGSDNQTSLEISNPSGETNLETSCFFSL
                     NNLLLGPTNSIRISADSELYQIKATELELPTALSKRNITLIPSLQIRFDPSSDSESIE
                     QIENQRISRLYRTDLPDPNRQQNDAAAPAFYTLNISVNGIPVEKSPASDETILVQWSL
                     FDQLAAENDLPSCLIPLGDGDYQMTFHSDWTAGCSFRIPMDFPINSAISPLRIQFLSP
                     LYPTETSISVAPEPFTVETAKAFVTVPNQIRLSENQQIAADINSASAPLSPYAAALIR
                     RLRGDLLGIQWEY"
BASE COUNT         1494 a         1287 c         1040 g         1396 t
ORIGIN      
        1 cttgtcatcg gtgaaaatcg tcaggaattg aactctgatc agtatcacgg aagtcaaacc
       61 gttaagattc aggaagacac cccagtccag ttaatttccg aaagtgaaat gggaaaagaa
      121 gttaaaaatt tggttttgaa agctgaaaat ccgcaaatgg aattggtcaa attcatcatt
      181 tggattcgcc cctctgctcc caaaacgaac acgtcgccgg ttcaacggat agacagttcg
      241 gatggatctt ccgacgaatt taccgccaaa tatgccgagc tggttcccga tccgagcagt
      301 ttaacgggtt ataaaatcgt tcgatatgaa tttgaccggc aaattatttc cggcgaacag
      361 attatgcttg aatgggaagt gaaggatgcc gaaaccgttc tgctggaacc tttatcggat
      421 caatttgttt ctcagaaagg ggctcaattc tattttccga cccaatcaac gaatttcacc
      481 ctctcggttt cagatggaag ccggctgaaa tcttatacct tgcccatttc catccgcacg
      541 gaaaagaatg tcgaggctgt cgcaccaaaa atcgatcttt tcgatgccaa tcctgttact
      601 ctcacgcagc cgggaaagac aacattatct tggtcagtct ccggagattg gacccgtatt
      661 caacttattg cgtccggtgc ctccttcgat tccattcatg ccgacacaaa taatgaaaat
      721 gccattcctt taacgatttc tcaaaacagt ggatccattc tcgccgatta tctcgcaccg
      781 accgggtttc tttctttgaa cctaaaagaa aacgcctctt ttattttgaa agcttggaat
      841 gggaatcttt caaacagttc gattgtcaat gtccgggtaa atccatcgga acctcccgcc
      901 gtattaaaat caccggacct gaaaattacc gcaattttca ccgattcagg caattatatg
      961 gttggagaca caataacagt gagcaccgct ttcgatgggc tatcctcaat agactctatt
     1021 ccaagcggca caattttaat cagtgacgga ctatctaact gcgtcattac gctgccgaag
     1081 aacagttgtg atttgaattt acgtgtcgcc ggaaatcgaa cgataaccgc tcaatattcc
     1141 ggaaatgcca gttaccgctc agcccaaact tctcaaaata ttaacgtaag cactgaaaat
     1201 aaaattcttc tggatatccg accttctcta tttccggaga aatcgatcta taccgtcgga
     1261 gaagagatta ccatcaccgc cggccttcct gattttctat ccgatcagcc tgccgcgacc
     1321 ggatcattgt ttataaccga cggaatctcc aattgcttat tgacgcttcc taaaaacaat
     1381 tgtgaattaa cgattcgcgc tgccgggaat caaatgatca ctgtcaatta ttccggagat
     1441 gaagattatt accctgctca agcgaaaatc agcattcgtg ccgaaaatca gatcccttcc
     1501 gcaacgccga gtccgcaacc gactctgaca ccgatcatca cgccgactat cgaaccgaaa
     1561 aacctctcat tgaaaatcgc cggcgtttat ccacagaaaa gctcttacca tatcggtgat
     1621 caaatagata tttatattga tccggattat gatgcgttaa gatccgattt tccgaaagaa
     1681 aaaatcagga tcagcgacgg ttatgccact tgtctttttt ctgtggaaag ccaaaatcat
     1741 tgttcgcttc aattgatcga tccgaccact acaaaaatca ccgcagcatt cccgggagac
     1801 ggattctatc tccccgtaac atcagagcct tggatcatca aagtggaaga acctccgaaa
     1861 gccaaaacga ccacttcaat tctcaattta aatccggtca aggataaata ccaaatcggc
     1921 gatatcgttc gagtctatgc cgcaaccgag cccgatcaat cctcggaaat cccgatcacg
     1981 ggtacgatta ctatcagcga ttcatacagt tcttgcgcca ttttattacc cgaccaaaaa
     2041 ttttgcgatt tgacactttc aaatggactt gcgaaggaaa ttacggcgat tttttcgggt
     2101 gatgatatct atgcatcttc cgtctcatcc gttaccccga ttcaaatttc catggtcgaa
     2161 gtttcggcaa tctccgttca ggccatgcag tattccgatt gtgaccattt catgcctgat
     2221 ccaaatcatg tgatccctct gaaaacggac acttctgttg tacccgaaga acactatttt
     2281 aatctggacg aacaatttcc gatcggcagt ggtttttata taaatgcaat cgttcgttca
     2341 aaagtcggtc acctcgatac ctctcccgga accttcaatg caaaaatttg cagtaaagaa
     2401 gaaccggatt cctgcgtaat ttccgacacg gttgaagccg tacgaaatca agatgatttt
     2461 acacaggcaa tgggaaggct tgaaatcccc ccaatatatc gtacgggaaa acatctcctg
     2521 accatcactt ttacgagtga tcccgaactt tacggtacgg aaacagccac tttcagcctc
     2581 tggaatgtcc aaagaggtca attggtactg acacctcatt ccgtaccgga gccggatcca
     2641 acctatgaaa attacctgtg gaacaacctg acggcgacgg agaagaattc cacggattat
     2701 ttattcgatg catatttact gaaaggaaat aacctccgat gcccggttgc ccttcaatct
     2761 tcgttgtatg aaaaaccttt cggcgtcacc ttgcaggttg aagtagatcc tgtccttccc
     2821 ggtgcaacaa taaccgattc ggaatggaaa gcagcgattc tttcaaatgg ctatgaagcc
     2881 gcaatggtgg ataaactgca gccgaacagc atgctttggg acagaaatac ctgtcgctgg
     2941 gaaacaaata acgagaactg gtttgtgcat tgcagtgatg tcggaatctc cgaaccgtca
     3001 acgattcgtt atgctttgga ttattcggac ccgaattaca taatcccgaa ccgacaaaat
     3061 ccggcggatg atttttctgc tgctacgata cgagtcgcta tcgggaaaaa attggctttt
     3121 attcattcca aggagctatt gggagcatta actccaaata ctccctattg gatcaattat
     3181 gaaggatcaa gcttcagttg gttcgcatca agcacagcat gcgatcagga accttaccga
     3241 ggcgactatg tttccaattc atccattcca gccgacaaga taattcacat tcatcttttg
     3301 cccctctctc gatcggcatt ggattcaaat ttcaaatggg agagaattcc cgcggaagtg
     3361 aaaattgatt acacgcccgc agaaaatcca ttcgctgttt tgagtactta tcatggtatt
     3421 tgcggaaatt gttccggaaa accgttgaaa aattttgatt atattcccaa tctgggatgc
     3481 gttcaaacag tagccggtca aatcaatcta tctccggaag aaggcacctt gctcgccgga
     3541 acagcctgcc cggacggaca acccaaaatg acgggaattc agtcaacgtt ttgccagctt
     3601 gagtttcaca ccgcatccga agtccacttc tctttttcgg gaaacgatga ctatcaaccg
     3661 gccgatcgac aagtcgccct caacataagt ggaatttccg cgagcggttc cgaacaatcc
     3721 gtccgattga tgtctgcccc cttgatgcca aatccttctt ccgaaccgca gccggtaacg
     3781 atccttccta cattcaccga tagtctcgga aatgtggttt atgagcatta cgtgggcgaa
     3841 aaatatatcc tccgattgca atcttccgta ccgttgaatg agaatatcat gctgattgcc
     3901 gaccttcccg atatcttttc aacagattca cttgatccgg atcaaagcag ctgcctaaaa
     3961 tcgatagatt tcgaagaaca tcagcttagg atcgcgggat cgaatttcac gggagagaac
     4021 aatactttcg actgtgagat cgtcttcaaa caaccggttt ctcccggaac aatccagacc
     4081 atttccttca tcctcgaaaa tatgaatgag aaaacaaatt atcggatgac tccgttcagt
     4141 tggagcggca ttcctcaatc cgtggtcaag aaagaggtat caatcgaagc aactgtcttc
     4201 aacagcaacg aaacaatttg cgataattta agcccctttt gcggagtaat gtatccttct
     4261 gaaagttatc agttaaagct gaaaatagct cttccgaacc ctcccgcgga aaatcagaaa
     4321 aaagttattt tccatatccg atggcccgaa ggaacgacca ttcggaacga aaattcagtt
     4381 cccaactcga attgcgaaat cggttcagac aaccaaacat ctttggaaat ttcaaatcca
     4441 tcgggagaaa caaatcttga gacttcctgc tttttcagtc tgaataatct gttactcgga
     4501 ccgacaaaca gcattcgaat ttccgccgat tcggaactgt atcaaataaa agcgacagaa
     4561 ttggaactcc cgacggctct ttcaaaacgc aatattacct tgatcccctc tcttcaaatc
     4621 cggtttgatc cttcatcgga ttcagaatcg atcgaacaga ttgaaaatca gcggatttcc
     4681 agactttatc gaacagatct tccggatccg aatcgccaac aaaatgatgc agcggcacct
     4741 gctttctata cgttgaatat ttcggttaac ggtatacctg tagaaaagtc acccgcctcc
     4801 gatgaaacca tcctcgttca atggagtttg tttgatcaat tggcagccga gaatgatttg
     4861 ccttcctgtt taatcccgtt gggagacgga gactaccaaa tgacattcca ttccgattgg
     4921 actgcgggtt gttctttccg aattcccatg gatttcccca taaattcagc aatctctcct
     4981 ttgagaattc aatttttatc acctctttat ccgacggaga cttctatatc ggtagccccg
     5041 gaacctttta cggttgaaac agccaaggct tttgtaactg ttccgaacca aatccgtctt
     5101 tctgaaaatc agcaaattgc agcggatata aattctgctt cagcgcctct ttctccgtat
     5161 gcagccgcat tgatcagacg gttgcgggga gatcttttgg ggattcaatg ggaatac
//
LOCUS       DAOMIV010000086         5217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_16119_length_5217_cov_4.236149, whole genome shotgun sequence.
ACCESSION   DAOMIV010000086 DAOMIV010000000
VERSION     DAOMIV010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16119_length_5217_cov_4.236149"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1213
                     /locus_tag="PKV59_03135"
     CDS             161..1213
                     /locus_tag="PKV59_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP45779.1"
                     /translation="MSENNNPSIQMTNTERILEVKHLKKFFPITKGFRKKHVGDVRAV
                     DDLNLYINKGETLGLVGESGCGKTTASRCILRAYEPTAGDILYRTKTGVTVNLSQMSN
                     KELRPLRAEMQMIFQDPYGSLNPRMNIFEIVSEPMLVNGIKNRREREDRVAELLTKVG
                     LRPEYMVRYPHSFSGGQRQRIGIARALALQPNLVVADEPVSALDVSVQAQVLNLLMEL
                     LEEMNLTYLFVAHDLSVVRHICDRVTVMYVGKVVETASTEALYNTPRHPYTEALMASI
                     PIPDPRLQQNREGLPGEVPNPSNPPSGCYFHPRCSYCIDKCKTEMPELKEIEPQHWTA
                     CHRAEELKLEGFHKNE"
     gene            1206..2387
                     /gene="nagA"
                     /locus_tag="PKV59_03140"
     CDS             1206..2387
                     /gene="nagA"
                     /locus_tag="PKV59_03140"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HQP45780.1"
                     /translation="MNDFQAVAIQNSKIIFPEKVEEGKVLLLRDGKIAGIVGRDDFNP
                     EDYLTIDAAGKYTAPGLIDLHVHGCYNHTFNESDPEAFDTILRNTLRYGITTLVPTLV
                     AAPIPNLIQSLCFIDKWKSEQKPGITQITGAYLESPYIAPQAAGAIPASALRKITDGS
                     IDQILELSYAISIFMIAPELEGATDAIRKIKQKGIIAAMGHSMAMESEIIPAIEAGAS
                     HVTHLWSAMSSVVRQGPWRKPGLLEVALVYPQLTTEIIADNRHLPPTLMKLAVKAKGD
                     MVCAVSDALNGAGLPEGSHFFVGENEYEVVDGVGMVADRSCFAGSTTLLGQEIPILVK
                     EVGLSIPEAIRMVTEIPAKILGIADKKGSIRTGLDADIILLDSNFKIHEIFQQGILIQ
                     F"
     gene            2418..3194
                     /locus_tag="PKV59_03145"
     CDS             2418..3194
                     /locus_tag="PKV59_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQP45781.1"
                     /translation="MEPIFTDQWGKLPVKVYETNEMMGEAAALDAREIINSAIAEKGF
                     ANLIIATGNSQLTFLKSLRELPDIDWGKVNVFHMDEYIGLPEGHTASFPYFLRKHFLD
                     YVKVKNFYPVPGNPKTLREDMLAYEKLLRENPADLCALGIGENGHIAFNDPPFADFDD
                     PVYIKKVRLDERSRKQQVGEGHFHSLDEVPMYALTLTIPALRSAKHMLCMVPESRKAE
                     AVYRTKTEPINENCPATILKDTPWCTLYLDKDAAEKILPK"
     gene            3213..3848
                     /locus_tag="PKV59_03150"
     CDS             3213..3848
                     /locus_tag="PKV59_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQP45782.1"
                     /translation="MKKQLFILGDSISIDYTPVLREYLGSEWEVLRKGDIPAPEISGE
                     IDHENGTDSNVVYEYLRLVLPFIQASLILLNSGLHDIKRMPNETDECQVSIERYRKNI
                     EKIIHLIQSEKKQALWVTSTPVDDQQHRKYETSFFRRNDDLVRYNRTAIEIMKKNNIP
                     VFDLYNFTSRINAPLYRDHIHFNNEIINLQAAFLSGCIQTFYSYGTRNNEE"
     gene            3849..5000
                     /locus_tag="PKV59_03155"
     CDS             3849..5000
                     /locus_tag="PKV59_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQP45783.1"
                     /translation="MNSRERLITTMNFKEPDRVPFDMGSTQVTGINEQAYRRLRSAYG
                     LPEKEVVYSDQIQGLALPDDDFIEALGIDIRGLFPLNAHNWNVKVTDGGKYWLYLDEW
                     GITHRKPKENGLYFSIYQEPLKNEKLSPDSIKNHPWPNFKDPQRIAGLKELADLYHSK
                     NYGVVMKDPFAGIFEMSQRICGMDYLLMLMASEPDLAGLLFDKMVELKIDFFSSALPV
                     LADSVDVIALMDDYGTQVSQLISPRMFRNQIKPRWKEVFDCIKQYAPNAKRFFHSCGN
                     VRPLIPDFIEIGVQILNPIHVKATGMDPYQLKKDYGDQLVFWGGGVDTQGILPMGTPE
                     EVREDVKRNLDALMPGGGYVFNTVHNIQADVPTENLVAMIETFKEYGVY"
     gene            5007..>5217
                     /locus_tag="PKV59_03160"
     CDS             5007..>5217
                     /locus_tag="PKV59_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45784.1"
                     /translation="MLKSDQFLPLEFVFNPNWWNKTAGISFDQDFYLNPKKREENDVI
                     MRRTLFEKFGEFGFGEENPKPRPIAG"
BASE COUNT         1617 a          997 c         1172 g         1431 t
ORIGIN      
        1 attcctcatc cgttcaataa accttccggt tgcccgtttc accctcgttg caataactgg
       61 attcgcggaa aatgtgaaaa atcactccct ccggagtacg tggtaggaga aggtcaaagc
      121 gccagttgtt tcttatatga aggatcggag gaggtgatac atgagtgaaa ataataatcc
      181 gtcgattcaa atgacgaata cagagagaat tcttgaagtg aaacatttaa aaaaattttt
      241 cccgattaca aagggcttca ggaagaaaca tgtcggagat gttcgggcag ttgatgatct
      301 caatttatat attaataaag gtgaaacttt aggattagtc ggtgaaagtg gatgcggaaa
      361 aaccacggct tctcgctgta tccttcgagc ctatgaaccg acggcgggag atattcttta
      421 tcgaacaaaa acaggtgtaa cagtaaattt gtcgcagatg tccaataaag aattacgacc
      481 gcttcgtgcc gaaatgcaaa tgatttttca agacccatat ggctctttga atccgcgaat
      541 gaatatcttt gaaattgtca gcgaacccat gctggtaaac ggtataaaga atcgacgaga
      601 gagagaggac cgcgttgcgg aattgttaac aaaagtcggg ttacgtccgg aatatatggt
      661 tcgatatccg cattcattca gcggcggcca acgccagcgg atagggattg cacgtgcttt
      721 ggcgttacag ccgaatttgg ttgtcgcaga cgagccggta tccgcattgg atgtttcggt
      781 tcaagcacaa gttttaaatc ttctgatgga acttttggaa gaaatgaatc tgacctatct
      841 gttcgttgct catgatttgt cggtcgtacg ccatatttgt gaccgggtta cggtgatgta
      901 tgtcgggaaa gtggttgaga ccgcttccac ggaagcgtta tataatactc cccgccatcc
      961 ttatacagaa gcactaatgg catccattcc gattccggat cctcgtctgc aacaaaacag
     1021 agaaggtctt ccgggcgagg ttccgaatcc ttcgaatccg ccgagcggtt gctattttca
     1081 tccccgctgc agctattgca tcgataaatg taaaaccgaa atgccggagc taaaagaaat
     1141 agaaccgcaa cattggaccg cctgccaccg agcggaagaa ctaaagttgg aaggatttca
     1201 taaaaatgaa tgattttcaa gccgttgcca tacaaaacag caagattatt tttccggaaa
     1261 aagtcgaaga aggaaaagtt ctcttattac gggatggcaa aattgccggg attgtcggtc
     1321 gggatgattt taatcccgaa gattatttga cgatcgatgc cgcggggaag tataccgctc
     1381 ccgggttaat cgatttgcat gtccatgggt gttataacca tactttcaat gaatctgatc
     1441 ccgaagcatt tgatacgatt cttcgcaata cacttcgata cggtataacg acgcttgtac
     1501 cgacacttgt tgccgctccg attccaaatt tgattcaatc gctttgtttt atcgacaagt
     1561 ggaaatcaga acaaaaaccc gggataacac aaataacagg cgcttatctg gaaagtcctt
     1621 atattgcccc tcaggcggca ggtgcgattc cggcatctgc tttacggaaa ataacggacg
     1681 ggtcaatcga ccaaatcctt gaattaagct atgccatttc gatttttatg attgctccgg
     1741 aattggaagg cgcaacggat gcaattcgca aaattaagca aaaaggaatc attgccgcca
     1801 tggggcactc gatggccatg gagtcagaaa taattccggc gattgaagcc ggtgcatccc
     1861 atgtaaccca tctatggagt gccatgtctt cggttgtcag gcaggggcct tggcgaaaac
     1921 cgggtttatt ggaagttgcg ctggtttatc ctcaactgac gacagaaatt attgcggata
     1981 atcggcattt accgccgaca ttgatgaagc tagcagtcaa agcaaaaggt gatatggttt
     2041 gtgccgtatc ggatgcttta aatggcgccg gtctgcccga aggatcacat tttttcgtcg
     2101 gtgaaaatga atacgaagtg gttgacggag taggaatggt agccgatcga agctgttttg
     2161 cggggagtac gacgttatta ggtcaggaaa ttcctatttt ggtaaaggaa gtcggattat
     2221 caattcctga agccattcgt atggttaccg aaataccggc aaaaattctt ggaatcgccg
     2281 ataaaaaagg atcgattaga accggtttgg atgcggatat tattttgctg gactcaaatt
     2341 ttaaaattca tgagatattt caacaaggaa ttctgattca attttaaaaa ttataaagaa
     2401 atataaagga aacaaaaatg gaaccaatat ttacggatca atggggaaag ttaccggtca
     2461 aagtttatga aacaaatgag atgatgggag aagcagcggc actggatgcg cgagaaataa
     2521 tcaattctgc gattgctgaa aaaggttttg caaatttgat tattgcaacc ggaaattctc
     2581 agctgacatt cttaaagtca ctcagagaat tacccgatat tgattgggga aaagtaaatg
     2641 tatttcatat ggatgaatat atcggtttgc cggaaggaca tacagccagt tttccatatt
     2701 ttttgcggaa acactttttg gactatgtaa aagtaaaaaa tttttacccg gttcccggaa
     2761 atcccaaaac tcttcgagaa gatatgctcg catatgaaaa attactaagg gaaaatccgg
     2821 cagatctttg tgcattggga atcggcgaga acggtcatat cgcctttaat gatccacctt
     2881 ttgctgactt tgatgatccg gtctatatta aaaaagtcag gcttgacgaa agatcacgga
     2941 aacaacaagt aggggaaggt cattttcatt ctctggatga agttccgatg tatgccttaa
     3001 ctttaaccat accggcttta cgatcggcaa aacacatgtt atgcatggtt cccgagagca
     3061 gaaaagccga agctgtatat cgaacgaaga cagaaccgat caacgaaaat tgcccggcta
     3121 ctatcttaaa agatactcct tggtgcacac tttatttaga caaagatgcg gcggaaaaaa
     3181 ttctaccgaa atagacagga tattactgct ttatgaagaa acaattattt attctcgggg
     3241 acagtatttc gattgactac acaccggtct taagagaata ccttgggagt gaatgggaag
     3301 tgcttcgcaa aggggatatc cctgcgccgg aaatttcagg ggaaatagat catgaaaacg
     3361 gaaccgattc gaatgtcgtt tacgaatatc tccgattggt cttaccgttt attcaagcat
     3421 ccttaattct tctgaattcc ggcctccatg atatcaaacg gatgccgaat gaaacggatg
     3481 aatgccaggt atccatcgaa aggtatcgaa aaaatattga aaaaataatt catctgattc
     3541 aatccgaaaa aaaacaagcc ctgtgggtca caagtacacc ggtagatgat cagcagcatc
     3601 gaaaatatga aacttcattc tttcgacgaa atgatgactt agttcgatac aaccgaacgg
     3661 caattgaaat aatgaagaaa aataatattc ccgtgtttga tttatataac ttcacctcaa
     3721 gaataaatgc tcctctttat cgagaccata ttcattttaa taatgagata atcaatcttc
     3781 aagccgcctt tttatcgggt tgtatacaaa cattttacag ttatggaaca agaaataatg
     3841 aggaatagat gaattctcgc gaacgattaa ttacaacaat gaatttcaag gaaccggatc
     3901 gtgtgccttt tgatatggga agcacacaag ttaccggaat aaatgaacag gcatacagac
     3961 gactgagatc cgcttatggc ttgccagaaa aagaagttgt ttattccgat caaattcagg
     4021 gactggcttt accggatgac gattttatcg aggctctcgg aattgatatc cgtggacttt
     4081 ttccattaaa cgctcataat tggaatgtta aggttacgga tggcggaaaa tattggttat
     4141 atcttgacga atggggaata actcaccgta aacccaaaga gaatggttta tatttttcga
     4201 tctatcaaga acctctgaaa aatgagaaat tatctcccga ttccattaaa aatcaccctt
     4261 ggccgaattt taaagaccct cagcgaatcg ccggattaaa ggaactggct gatttatacc
     4321 actcaaaaaa ttacggcgtc gtgatgaaag atccttttgc aggcattttt gaaatgtctc
     4381 agcggatttg cggcatggac tatttgttaa tgttgatggc aagtgaacct gatcttgcgg
     4441 gtctcctctt cgataaaatg gttgaattga aaatcgattt cttttccagt gcattacctg
     4501 ttttggccga ttcagtggat gttattgcat tgatggatga ttacggaact caggtttctc
     4561 aattgatttc accccgaatg tttcgcaatc agattaaacc tcgttggaag gaggtttttg
     4621 attgcattaa acaatatgca cccaatgcca aacgcttctt ccattcgtgc gggaacgtca
     4681 ggccgttaat tccggatttt atcgaaatcg gcgtccaaat attaaatcct attcatgtta
     4741 aagcaaccgg aatggatccc tatcaactca aaaaagatta tggagatcaa ttagtatttt
     4801 ggggcggagg agttgataca cagggaattt tgccgatggg tacacctgag gaagtcagag
     4861 aagatgtaaa aagaaatctt gatgctttaa tgcccggagg cggttatgtt tttaatacgg
     4921 ttcataacat tcaagcggac gtgccgacag aaaatctggt tgcaatgatt gaaacattta
     4981 aagaatatgg agtctattaa atcaaaatgc taaaatccga ccaatttctt ccgcttgaat
     5041 ttgttttcaa tccgaactgg tggaataaaa ccgccgggat ttcctttgat caggattttt
     5101 atttgaatcc aaagaagaga gaagaaaatg acgtaattat gcgccggaca ttatttgaaa
     5161 agttcggaga atttggtttc ggtgaagaaa atcccaaacc gcgacccatt gccggat
//
LOCUS       DAOMIV010000087         5105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_16579_length_5105_cov_3.544950, whole genome shotgun sequence.
ACCESSION   DAOMIV010000087 DAOMIV010000000
VERSION     DAOMIV010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16579_length_5105_cov_3.544950"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /locus_tag="PKV59_03165"
     CDS             complement(<1..148)
                     /locus_tag="PKV59_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQP45785.1"
                     /translation="MNLKPLGNRLVVEPIEEEEMTAGGIVLPETAKEKPQKGKVISAG
                     PGDRN"
     gene            350..1096
                     /gene="trmD"
                     /locus_tag="PKV59_03170"
     CDS             350..1096
                     /gene="trmD"
                     /locus_tag="PKV59_03170"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQP45786.1"
                     /translation="MKFDVFTLLPGVFEDYLNSSILLKALENQLIEVAVHDIREWAPG
                     KHHVTDEPPYGGGGGMVMKPEPVFNAVEAVLGDKPDCPVILLTPQGKVFDQKTAQRLA
                     REPHLALICGRYEGFDERIRVHLATEEISIGDFVLTGGELPALVLIDAISRNIPGVLG
                     DPDGAMTDSHSEGLLEYPHYTRPAVFRDWEVPSILLSGNHAAIENWRREQSLARTLER
                     RPELLDRAELSETDRQILQKLGWKPKERSA"
     gene            complement(1118..2230)
                     /gene="tgt"
                     /locus_tag="PKV59_03175"
     CDS             complement(1118..2230)
                     /gene="tgt"
                     /locus_tag="PKV59_03175"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQP45787.1"
                     /translation="MNSDNFPFEIEGKDGKARAGVFYTPHGSFQTPIFAPVGTQATVK
                     SLTPVQVEEAGATLILANTYHLFLRPGDDLIAEMGGLHRFMDWQKPILTDSGGFQVFS
                     LDGMRKIDEDGVTFKSYIDGSVHRFTPEISIAVQENLGADIIMAFDECANPKDYEYNI
                     RSMDRTHRWLQRCIDAKTNDKQALFGIVQGGIFPDLRTKSAEFVSSFDLPGFAIGGLS
                     VGETKEEMNAVLDIVPEILPENKPRYLMGVGSPEDLINGILRGVDIFDCVLPTRLARH
                     GSAMSFTGRINLNNQQYARDPRPIVEGCDCYTCRHFSRSYIRHLLISKEMLSGTLISI
                     HNIHTLEELVRQSRRAILSGTFSRYAEDFLTAYGSN"
     gene            complement(2309..2707)
                     /locus_tag="PKV59_03180"
     CDS             complement(2309..2707)
                     /locus_tag="PKV59_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQP45788.1"
                     /translation="MLNIGIKGTKTETVTNENTAAHLGSGDLQVYGTPAMIALMESAA
                     LESVQPYLEPGTTTVGVRLEVEHLAPSPLGMKISCESTLIEIDRRMLTFEVSVFDEKG
                     EIGRGIHKRFVVQRDAFLQKALARKANGSS"
     gene            complement(2726..3649)
                     /locus_tag="PKV59_03185"
     CDS             complement(2726..3649)
                     /locus_tag="PKV59_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HQP45789.1"
                     /translation="MDIRYAILGLLQNTRLSGYDLKKAMARSPLLYWSGNNNQVYRTL
                     AELEKEDFVVGEVEKKDTLPLKKFYSITENGKKELQRLSRDFPEMPEIRKPFSIQLLF
                     GGDLSKTEAESLLDRYADEIEGTIAMASDENPLADPTPYEAVLSKLATDNIRRFYESE
                     LLWVEKVRSEALPLLPDESDACRKKEYDMKMETIKVQKNGKDYVKVISGKINNEKDGL
                     ALVSACIENDTNRLLLPPEALSDAFYDLSTQLTGLVLQKLVNYRIRAAAVIEPAKVRG
                     KFKDFMIETNRGQMFRLFEDFKQAEDWLLKD"
     gene            3911..>5105
                     /locus_tag="PKV59_03190"
     CDS             3911..>5105
                     /locus_tag="PKV59_03190"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQP45790.1"
                     /translation="MTKYIFVTGGVVSGLGKGITAASLGRLLKARGLKVAAQKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERFIDEDLNKYSNLTTGKVYWNVLNKE
                     RNGEYLGETIQVIPHITNEIKSFVYSVGQKTNADVVITEIGGTAGDIESQPFLEAIRQ
                     IGLEVGQENCLFIHVTLVPYLKSSGEQKSKPTQHSVRELQSMGIFPKIIVLRCDEPIE
                     PGIVQKISLFCNVKPDCVIENQTIPVLYEAPIMLEKSHFSEIVCRELNINAPDINLTE
                     WNKMLDRIRLCDKVVKIGLVGKYVKLHDAYLSVMEALQHAGYELGTKIKIKWIDSETL
                     KPGNYEKVLSDCAGIIIPGGFGNRGIEGKILAARYARENNVPYLGLCLGMQVAVIEFA
                     RNVCGL"
BASE COUNT         1328 a         1141 c         1221 g         1415 t
ORIGIN      
        1 cattgcggtc gcctggaccg gcagaaatga ctttcccttt ctggggtttc tcttttgcgg
       61 tttcgggaag gacaatccct ccggcggtca tttcctcttc ctcaatgggt tctacaacta
      121 atcggttacc taatggtttt aaattcattt tattcctcca actatggctt ttattgttct
      181 ttttcttaaa tttagcactc tttaatactg agtgctaatg cacgacatat aataatccgc
      241 taaaaagaga ttgtcaaggt gaaatttatg attctaataa aactcttaca tttcttatga
      301 tcacattttc tctatttaca aagttttcct tcgaccataa aatgggggta tgaaattcga
      361 tgtgtttacg ttactccccg gtgtatttga ggattacctc aacagcagta ttttattaaa
      421 agcactggag aatcagttga ttgaagtggc cgttcacgat attcgtgaat gggcgccggg
      481 aaaacaccat gtgacggatg aaccgccgta cggcgggggt ggcggaatgg tgatgaaacc
      541 agaaccggtt ttcaatgccg tggaagcggt tttaggagat aaacccgatt gtccggttat
      601 tttattgacg cctcagggga aggtattcga tcagaaaaca gctcagagat tggctcggga
      661 acctcatctg gcattgatat gcggccgtta tgaagggttt gatgaacgga ttcgtgtaca
      721 cttggcgacg gaagaaatat cgatcggtga tttcgtgtta accggagggg aattgcccgc
      781 gttggtcctg atcgatgcga tcagccgaaa tataccgggg gtattaggcg atccggacgg
      841 agcgatgacc gattctcatt cggaagggtt attagagtat ccgcactata cgcgtcccgc
      901 ggtcttccgc gactgggaag taccttccat actgttaagt ggaaaccatg ccgccatcga
      961 aaactggcgt cgcgaacaat cgctggcacg cactttggaa cgccggccgg aattattgga
     1021 tcgggccgag ttatcggaaa ccgatcgaca aatattacag aaattaggtt ggaaaccgaa
     1081 agaacgatcc gcctgaaaaa tcgtttcgat tgcggagtta attcgaaccg tatgcggtga
     1141 ggaaatcttc cgcgtatcgg ctgaaggtac ccgaaaggat cgcacgccgc gattggcgaa
     1201 cgagttcttc cagtgtatga atattatgaa tggaaattaa ggtgccgctg agcatttctt
     1261 tggaaattag aaggtggcgg atataactgc gtgaaaaatg ccggcaggtg taacagtcgc
     1321 aaccttcgac gattgggcgg ggatcgcggg cgtattgttg attgtttaaa ttgatgcgcc
     1381 ccgtaaaact catggctgaa ccgtgtcttg ccaaccgagt cggcagaacg caatcaaaaa
     1441 tatccactcc gcgcagaatt ccgttaatca aatcttcggg ggaaccgacg cccatcaaat
     1501 agcgcggttt attttcgggc aaaatttctg ggacgatatc caaaacggca ttcatttctt
     1561 cctttgtttc gcccacggac agacccccga tcgcaaaccc gggtaaatca aaggagctga
     1621 caaattccgc ggattttgtt cttaggtcgg ggaaaatacc gccttgcacg attccaaaca
     1681 gcgcttgttt atcattggtt ttcgcatcga tgcagcgctg cagccagcgg tgggtgcgat
     1741 ccatagagcg gatgttatat tcataatctt tcggatttgc gcattcatca aaagccatta
     1801 taatgtccgc gcctaaattt tcctgaacgg ctatggatat ttccggggtg aaacggtgaa
     1861 cggatccgtc gatgtaactt ttaaaggtta cgccgtcctc atcaatttta cgcattccgt
     1921 ctaatgagaa aacctgaaaa ccgccggagt cggtcaggat gggtttctgc caatccataa
     1981 agcgatgcag ccctcccatt tccgcgatca aatcatctcc gggacggaga aacaggtgat
     2041 aggtatttgc caaaatcagc gtggcgcccg cttcttccac ttgtaccgga gtcaatgact
     2101 tgacggttgc ctgtgttccg accggagcaa aaatcggtgt ctgaaacgaa ccgtgcggtg
     2161 tgtaaaatac cccggctcgg gctttgccgt ctttgccttc tatttcgaag ggaaaattat
     2221 ccgaattcat aacagcggca attgtaccat ctgtgtcaat ttaaaggttt tttccgggaa
     2281 gtcgtaatta tttttattcc gcttgttatc aggaagaacc gtttgctttc cgcgccagag
     2341 ctttctgcaa aaaagcatcc ctttgaacga caaatcgctt atgaattcct ctgccgatct
     2401 cccctttttc atcaaagaca ctgacctcaa aggtcagcat gcggcgatcg atttcgatca
     2461 gcgtgctctc gcaggaaatt ttcattccca gcggagaagg ggcgaggtgc tcgacttcca
     2521 atcggacccc gaccgttgtt gtaccgggct cgagatacgg ctgcacactc tcaagcgcgg
     2581 cggactccat taatgcaatc atagccgggg taccgtagac ttgcaaatct ccactgccga
     2641 gatgggcggc ggtattttcg tttgtgaccg tctccgtttt tgtgcctttt ataccgatat
     2701 tcagcattgt ttcatccttt ctgatttaat ctttcaacag ccaatcttcc gcctgcttaa
     2761 aatcttcgaa caaacggaac atttgtccgc ggtttgtttc gatcatgaaa tccttgaatt
     2821 ttccgcggac tttcgcaggc tcgatcaccg cagccgctcg aatccgatag ttgacaagtt
     2881 tttgtaagac caatcccgtc aattgtgtgg aaagatcata aaacgcatcc gaaagtgctt
     2941 ccggcggcaa caacaaacgg ttcgtgtcgt tttcaataca agcagacacc aatgccaacc
     3001 catccttttc gttattgatc ttgccggaaa tgactttcac ataatctttc ccgttttttt
     3061 gtacttttat cgtttccatt ttcatatcat attctttctt tcggcatgca tcggattcat
     3121 cgggtaggag cggaagcgct tccgatcgca ccttttcaac ccataacaat tcggactcgt
     3181 agaaccggcg gatattatcc gtcgccaact ttgacaagac cgcttcgtaa ggagtgggat
     3241 cggcgagagg attttcatcc gatgccatcg caattgttcc ttcgatttcg tccgcatatc
     3301 gatccaacaa agattctgcc tcggtcttcg agagatctcc gccgaacagt aattggatcg
     3361 agaaaggttt gcgaatttcc ggcatttcag gaaaatcccg ggaaagtctt tgcaactctt
     3421 ttttaccgtt ttcggtgatc gaatagaact ttttcaacgg aagcgtatcc ttcttttcga
     3481 cctcgccaac cacaaaatcc tctttttcaa gctccgcaag cgtgcgatag acttgattat
     3541 tatttccgga ccaataaagc agaggcgaac gggccatcgc ttttttaaga tcatagccgg
     3601 acaagcgggt attctgcagc aatcccaata tggcatatcg gatatccaat tccgtttctc
     3661 ctttcaagat atatgttaat aataacatat tatctttatg caatcaaaaa atcggtccga
     3721 gagaatttct tcggttaaat gatctgctgt ttcggaattc ggattttccc caatttatca
     3781 gaaaaatatt gattttaaaa ttgaatcggg taaaatcaaa aaggtacaca tttttctgta
     3841 aattgttggc gaattgagcg tttgtaccga cttaatcgaa ttatttaagc ttgcatcggg
     3901 agaaaaatcg atgacgaaat atatttttgt gacgggaggt gtggtttccg gtttagggaa
     3961 aggaattacc gccgcatctc tcggcagatt gttaaaagcg cggggtctga aagtggctgc
     4021 acaaaaactg gatccgtata tcaacgtgga ccccggcaca atgagcccgt ttcagcacgg
     4081 agaagtcttt gtgacagagg acggtgcgga gacagatttg gatttgggac attatgaacg
     4141 gtttatcgat gaggatctta acaaatattc caatttaacg accggcaagg tttactggaa
     4201 tgtcttgaat aaagaacgga acggtgaata tctcggtgag acgattcagg tgatcccgca
     4261 tatcacgaat gaaatcaaat cctttgtcta ttccgtcgga cagaaaacaa acgccgatgt
     4321 ggtaataacc gaaatcggcg gaaccgcggg ggatatcgag agccagccgt ttctggaagc
     4381 gattcgtcag atcggtttgg aagtcggtca ggagaactgt ctgtttattc atgtgacatt
     4441 ggttccttat ctcaagagtt ccggagagca gaaatccaaa ccgacgcagc attccgtccg
     4501 tgaattacaa tcgatgggaa ttttcccgaa aattattgtt ttgcgctgcg atgaaccgat
     4561 cgagcccggg atcgttcaga agatttcgtt gttctgtaac gttaagccgg attgtgtgat
     4621 cgaaaatcaa acgattccgg tgctttatga agcaccgatt atgcttgaaa agagccattt
     4681 ttccgaaatt gtctgccggg aattgaatat aaacgctccg gacatcaatc tgaccgagtg
     4741 gaataaaatg ctggatcgga tccgtctctg tgacaaggtc gtcaagatcg gtctggttgg
     4801 gaaatatgtc aaactgcatg acgcctacct ttccgtgatg gaagcgttac agcatgccgg
     4861 atatgaattg ggaacaaaaa ttaaaatcaa atggatcgat tcggagacgt taaaacccgg
     4921 aaattacgaa aaagttcttt cggattgcgc cggcatcatt attccgggcg gtttcggcaa
     4981 tcgcggaatc gaagggaaga tcttggcggc tcgctatgcc cgagaaaata atgtcccgta
     5041 tttgggatta tgcctgggca tgcaggtagc cgtaatcgag tttgcgcgca atgtgtgtgg
     5101 attgc
//
LOCUS       DAOMIV010000088         5043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_16841_length_5043_cov_4.020850, whole genome shotgun sequence.
ACCESSION   DAOMIV010000088 DAOMIV010000000
VERSION     DAOMIV010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16841_length_5043_cov_4.020850"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PKV59_03195"
     CDS             complement(<1..461)
                     /locus_tag="PKV59_03195"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQP45791.1"
                     /translation="MKLYEYQSKGFFKKYGIPIPAGHVTSIAREAKQIAEEIGLPVVV
                     KAQVLAEGRGMAGGIRLAKTPEETEIFASEILSAKIHGLPVQKVLIDEALSIEKEYFI
                     LISIDRAKKRPLLMMYDSGTIYIEAAANALFENLDRVYIDPLIGLQRYQIH"
     gene            complement(480..1538)
                     /locus_tag="PKV59_03200"
     CDS             complement(480..1538)
                     /locus_tag="PKV59_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQP45792.1"
                     /translation="MARIFDVVEYPSEMTDEIVHRFPETGVADLRFGSQVIVRESQRA
                     IFYRDGKALDQLGPGRHTISTANIPLLTALLGVAFNHRTPFTAEIYFVSMREFADVKW
                     GTSQPIIVRNPGVGLGISLLQGYGTYSFQVSDPQQFVTQIVGASQSYSLSAIEDRLRA
                     MLLSKLQDTLGVITGEQNADVMKLIGLVEEIGAAVRAKAQDDFAAIGLALKSFYIASL
                     KPSEKSAEELRAMGMLDMDVYTQLQAADALREAAKNPSGGAGLTAGIGAGIGLGDIIA
                     NTYRNQPPAQSNASAAADQPATAESGEVLTPAEAAKLLKVSEEDVIASIESGELKARK
                     IGSAYRISRESLNEFLKG"
     gene            complement(1542..2093)
                     /locus_tag="PKV59_03205"
     CDS             complement(1542..2093)
                     /locus_tag="PKV59_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45793.1"
                     /translation="MENLNDLVKSGKNSFQELMEKIPGIKGYKAKEDRRDTDKIIREE
                     IAKKFEEEWSRISAIQRDLIKAGNLNAVSDLESAAIKIRQFTDRIKTAPYGYAGLLDA
                     IKVDDAALTRLYDYDLYLLKMADTVKASVDEVEKAVASDPDNIKNAISQLTKAAQDSL
                     TAFNQRTEVIIGASDLNSGKSGE"
     gene            complement(2081..2236)
                     /locus_tag="PKV59_03210"
     CDS             complement(2081..2236)
                     /locus_tag="PKV59_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45794.1"
                     /translation="MRVLSATDKVKLGRTAVLTGSDLRKLLIIERMNNLKEKYAGMED
                     KEDKWKI"
     gene            complement(2248..3474)
                     /locus_tag="PKV59_03215"
     CDS             complement(2248..3474)
                     /locus_tag="PKV59_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQP45795.1"
                     /translation="MKKRFWLQISILLIFLTVTNGFAEDDHLSAVKTNRILYFGVAPD
                     FFPFVYHNEAGELTGLDIDLMQEIGRRMGVAVENIEIAYEGLPAALAVHQVDVIGGAL
                     EPGEMSSEMLDFSRIYFLGNYGLMILGNSEIPDSIREEDLYWMRLGVEKGGAMDQFVK
                     TKLIGRGLIDFQQVIYFDTVAEAVDALNWNEVDLILMARNTYKRQYEIDGSYRFISPE
                     WLKENYAFASYKGSNLIPEINRLLDLIMADGTAQKLAGKYGNLISSHPIAVSPDRPEE
                     NQEEMSSAKNGEEPCLNAMRFLADVTVPDGTKIPRDSEFEKTWRVYNIGTCTWTKDYF
                     LKYKDGASLGVQRIPVVEEVSPGSDYEFTAVFKTPSETGMTRTSWQMMTPEGKSFGQT
                     VWVNIEVTDDGFSKSN"
     gene            3627..4811
                     /gene="larC"
                     /locus_tag="PKV59_03220"
     CDS             3627..4811
                     /gene="larC"
                     /locus_tag="PKV59_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HQP45796.1"
                     /translation="MKILYCDCYSGISGDMFLAAMLDAGYPLEDLEKVFSLIELPEYQ
                     KVSFRKVQKGAISAGQVTFVLREDPHHHTGDLIHGHSHRSFADIRKLITGSNLPESIV
                     AKSLRIFEKIAQAESSVHNVPVDEVHFHEVGATDSILDIVGAAAALDYFGIDQIFSSP
                     LPLGNGEVMTQHGLLPIPAPATAWLLRDMHAKIKPSSVTKELVTPTGAGILAAFAEFD
                     QPEMTLTHYGTGAGSADFPHPNILRVFIGDSEAESESCIELSTNLDDMTPEQLGSVLN
                     RILEAGALDAFFTPIQMKKNRPAVQLSVIANRADEDKISELILRQTTTLGVRVHAVRR
                     HKADRKIVTLETEFGPVRAKTKIMNGETLSVHPEYDECERISAQTGLPLLEVQRRILK
                     HV"
BASE COUNT         1295 a         1296 c         1080 g         1372 t
ORIGIN      
        1 tgatgaatct gataacgctg caaaccgatc aacggatcga tgtaaacccg atccaagttt
       61 tcaaacaaag cgtttgcggc tgcctcgata taaattgttc ccgaatcata catcatcaac
      121 agcggtcgtt tttttgcccg atcgatggaa atcaaaataa aatattcttt ttcaatggac
      181 agcgcttcgt caatcaggac tttctgaacg ggaagcccgt gaatcttcgc cgaaagaatt
      241 tcggaagcaa aaatctctgt ctcttcgggg gttttggcta aacgaatccc gccggccatc
      301 ccccggccct cggctaagac ttgggctttg acgacgaccg gcaatccgat ttcttcggca
      361 atttgttttg cttcccgtgc gattgatgta acatgtccgg ccggaatcgg tattccgtac
      421 tttttaaaga aacctttaga ctgatactca taaagtttca ttgttttttg gttgtccgct
      481 tatcctttca gaaattcatt cagagattcc cgactgattc gataagctga gccgattttt
      541 cgtgctttca attctcccga ttcgatggaa gcgataacat cctcttccga tactttcaat
      601 aattttgccg cttcggcggg agtcaatact tcaccggatt ccgcggttgc tggttgatcg
      661 gcagcggctg aagcgttact ttgtgcgggg ggttggttgc gatacgtatt tgcgataata
      721 tctcccaagc cgataccggc accaattccg gcggttaatc ccgcaccgcc gctcggattt
      781 ttcgcagcct cgcgcagtgc atccgccgct tgaagttggg tatatacgtc catgtcgagc
      841 attcccatcg cccgcagctc ttccgccgat ttttcggagg gtttcaaact ggcgatataa
      901 aaactcttca gcgccaaccc gatggcagca aaatcatctt gagctttcgc ccggacggcc
      961 gcaccgatct cttccaccaa cccaatcagc ttcatcacat ccgcattttg ttccccggtt
     1021 atcacaccca aggtatcctg caatttggag agcagcatcg ctcgcagccg atcttcgatc
     1081 gcactcaacg aataggattg tgaagcgccg acgatctgag tgacgaactg ctgcggatca
     1141 ctcacctgaa aactgtaggt cccataccct tgcaataaac tgatgcccag ccccacgccg
     1201 gggttccgaa cgatgatcgg ttgtgaagtc ccccatttga catcggcgaa ttcccgcata
     1261 gagacaaaat aaatttctgc cgtaaacggc gtccgatgat taaatgcaac gcccagtaaa
     1321 gcggttaaaa gcggaatgtt agccgtagaa attgtatggc gtccgggtcc caattgatcc
     1381 agcgcctttc cgtctctgta aaatatcgcc ctttgggatt cgcgaacgat gacctgagaa
     1441 ccgaatctta aatcggccac cccggtctcg gggaaacggt gaacaatttc atccgtcatt
     1501 tcacttggat attcaactac atcaaaaatg cgagccattc tttattctcc tgattttccc
     1561 gaattaagat cacttgcacc gattatgact tcggtacgct ggttaaatgc cgtcaggcta
     1621 tcctgcgcgg cttttgtcaa ctgactgatc gcgtttttaa tattatccgg atcggatgcg
     1681 accgcttttt ccacttcatc tacggaagct ttgacggtat ccgccatctt cagtaagtac
     1741 aaatcataat catatagccg tgtcaaggcg gcgtcatcca cttttatggc atccaacaat
     1801 ccggcgtatc catagggggc tgtcttgatt cgatcggtaa attgccgaat tttaatcgcc
     1861 gcactttcca aatcggaaac cgcattgaga tttccggctt taatcaaatc acgttgaatc
     1921 gccgaaatcc tggaccattc ttcctcgaat tttttggcaa tttcttcacg aataattttg
     1981 tctgtatcac ggcgatcttc tttcgcctta tatcccttaa tcccagggat tttttccatt
     2041 aattcctgga aactgttctt cccggacttg acgaggtcat ttaaattttc catttatcct
     2101 ccttatcttc catccccgca tatttttctt ttaaattgtt cattctttcg ataatcaata
     2161 atttccgtaa atcgctgcct gttaagacag ccgttcttcc caattttacc ttatccgtcg
     2221 cagacaaaac tcgcaccaag cgccgtgtca attgcttttt gaaaacccat catccgtaac
     2281 ctcaatattg acccaaacgg tttgtccgaa ggatttccct tctggagtca tcatctgcca
     2341 actcgtcctt gtcattcccg tttcggaggg agttttaaag acagcggtaa attcatagtc
     2401 ggaaccgggg gaaacttctt cgacaaccgg tatacgttgt acgcccagag acgctccgtc
     2461 cttatatttc agaaaataat cttttgtcca ggtgcaggtt ccgatgttgt agacccgcca
     2521 ggttttctcg aattcgctgt cgcgagggat ttttgtcccg tccggaaccg ttacatcagc
     2581 aagaaaacgc atggcattca gacagggttc ttctccgttt ttcgcagagc tcatttcctc
     2641 ttgattttct tccggacgat ccggcgaaac cgcaatcgga tgggaagaga ttaaattccc
     2701 gtacttaccg gcgagttttt gcgccgtgcc atccgccatg atcaaatcca ataaccgatt
     2761 gatctcgggt atcaaattcg aacctttata agaagcaaaa gcataatttt ccttcagcca
     2821 ttcgggcgaa ataaagcgat aagaaccgtc tatttcatat tgtcgcttat aggtattccg
     2881 agccatcaaa atcaaatcca cttcattcca attcaaagca tcgaccgctt ctgcaacggt
     2941 atcgaaataa atcacctgct gaaaatcgat cagtccgcgt ccgatcagtt tggtcttgac
     3001 aaactgatcc atcgcaccgc ccttttcaac accgagacgc atccagtata agtcttcctc
     3061 tctgatcgaa tcggggattt ccgaatttcc taagatcatc aatccataat tgcccagaaa
     3121 ataaattcgg gagaaatcca acatttcgct cgacatttca ccgggctcca atgcaccgcc
     3181 gatcacatcg acctgatgaa ccgccagagc ggccggcaga ccttcataag cgatttcgat
     3241 gttttcgacc gcgacgccca tccggcgccc gatttcttgc attaaatcga tatccaatcc
     3301 ggtcaattca ccggcctcgt tatgataaac aaaagggaaa aaatcggggg cgactccaaa
     3361 atacaggatt cgatttgttt tcactgccga cagatgatca tcttccgcaa agccgttcgt
     3421 aacagtcaga aagatcagca aaattgagat ttgaagccag aacctttttt tcaaaaccag
     3481 ctccttttct taaatcgaaa cccggaaaaa tccgctcaaa aaaagacatt cataattaga
     3541 ttatagcatt ttgcactaaa tcaggttaac attatgcgaa ataaaagaaa tcggcagcaa
     3601 caaatatctt tgatcaagga ctcgttatga aaattcttta ttgcgattgt tattccggaa
     3661 tcagcggtga catgtttttg gcggcgatgt tggacgccgg atatcccttg gaggatcttg
     3721 aaaaagtctt ttcgcttatc gaacttccgg aatatcaaaa agtcagtttt cgaaaagtgc
     3781 aaaaaggagc catcagtgcc ggtcaggtaa cgttcgtcct gcgggaagat ccccatcatc
     3841 ataccggtga tctcattcac gggcattcgc atcgttcctt tgccgatatt cgcaagctga
     3901 ttaccgggag caatcttccc gaatcgattg tcgccaaaag tctgcggatt tttgagaaaa
     3961 tcgcccaagc cgaaagttcg gtccataatg ttcccgtcga tgaggttcat ttccatgagg
     4021 tcggtgcaac ggattcgatt ctggatatcg tcggcgcggc ggcagccctg gattatttcg
     4081 gaatcgatca aatcttttcc tctccgctgc ctctcggcaa cggagaagtc atgacgcaac
     4141 acggtctgtt gccgattccg gctcccgcaa ccgcttggtt gttacgggat atgcatgcca
     4201 aaattaaacc ttcttccgtc accaaagagc tggttacccc taccggagcc ggcattttgg
     4261 ctgccttcgc cgaatttgac caaccggaaa tgactctgac tcattacggc accggtgccg
     4321 gttcggcgga ttttccccac ccgaacattt tgcgtgtgtt tatcggggat tctgaagcgg
     4381 aatccgagtc ctgcatcgaa ctctccacca atctggatga tatgacaccg gaacaattgg
     4441 gatcggtttt aaatcggatc ctggaagcgg gagcgttgga tgcatttttc acgccgattc
     4501 agatgaaaaa aaatcgcccg gccgttcaat tgtcggttat cgccaaccgt gcggatgagg
     4561 ataaaatcag cgaattaatc ctgaggcaga caaccacgtt gggcgtacgc gttcatgccg
     4621 ttcgcagaca caaagcggat cgcaaaatcg tcactttgga aacggaattc ggacccgtcc
     4681 gcgcgaaaac aaaaatcatg aacggggaga cgctttccgt tcatcccgaa tatgatgaat
     4741 gtgagcgtat ttccgcacag acgggacttc ctttactgga agtgcaacgc cgaatcttaa
     4801 aacacgtttg atcctgttca aaagctgata cggggatgaa cgccgacggg atacagggag
     4861 ttgtcgcctg agaagcttaa atgtttctta aatcaggtgc ctcatgtttg cgaatcttcg
     4921 gcattcgagc atccagatat ccgatgggat agtcgattct tttcatcgat tggaaatgag
     4981 cgaaaacgga cggacggatt gacaaccctt attaaaaaag aatacaataa gaaaaaggca
     5041 cca
//
LOCUS       DAOMIV010000089         5005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_17029_length_5005_cov_4.550303, whole genome shotgun sequence.
ACCESSION   DAOMIV010000089 DAOMIV010000000
VERSION     DAOMIV010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17029_length_5005_cov_4.550303"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /locus_tag="PKV59_03225"
     CDS             <1..1190
                     /locus_tag="PKV59_03225"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQP45797.1"
                     /translation="SELLIIFGTVLFIALSRLFSFEGIFTASRSPILSVSLPWIAGWF
                     ILFFIFYYFGGSKPKKLFSLFIGFILLILILLKTPRLALQVSRFFRNLAGQSAELATA
                     NDLRWFGFSYIAFRLLSVLIDARKGRKWVATPGDFLIYVCFPPALAAGPIDRLDHFDK
                     ELNSVPHPFPEDFREGIPRIATGLFKKFILADSLALVALSSQNAVLIKHTGWAWASLF
                     AYSLQLYFDFSGYSDVAIGLAHLIGIKLPENFNHPYLKPDIAKFWNSWHITLTQWIRS
                     YTFNPLVRKLRKKKDPAFPQWFMILITQSVTMGLIGLWHGVTLNFFIWGIWNALGLFF
                     HQLYADHTKNQLMVISEEHSIAFRIYTIGSTLLTFLFVSLGWIWFVLPNIDSAALFFE
                     RLF"
     gene            1198..2301
                     /locus_tag="PKV59_03230"
     CDS             1198..2301
                     /locus_tag="PKV59_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45798.1"
                     /translation="MKKILSFTLKVFFFLIILLALYYLIQPEPFLGKISLYNFIFPGR
                     ERLPFGENPRVAYNFSLYNLNAMFASHEISGVQEQDNSFRVITIGDSSVWGTLLKPEE
                     TLAGQLDGKKIKIHGAERKLEVYNLGYPTLSLAKDVLILQRGMAYKPDLILWFTTMES
                     FPNEKQIESPLLAKNSEEFAQLLTSYGEIPSWMPKSSPESFWEKTLFSQRRALMDLIR
                     LQLYGFMWAATGIDQDYPADYPMAQVDFDRDNSFHGVDGIYPNDQMAWELLNVGMRIA
                     GNVPVILINEPILISDGENSDIRYNFYYPRQAYDVWRKDLADRCTEEKWKCLDLWDLL
                     SQSDFTNSAIHYNSEGGQKVVDTILDVLPESLQ"
     gene            2311..3180
                     /locus_tag="PKV59_03235"
     CDS             2311..3180
                     /locus_tag="PKV59_03235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQP45799.1"
                     /translation="MKKPPSCSKNLFFLILSLPFFFSVPSFAQSIEDRRPSDFGRTWQ
                     DLPIVPELNENSLRILKAGIAAGHNPHAFSKVGDCDTSTDWYLADFDKDSRYYNLGEY
                     RDRFEPVLSYYKGSFSHRSLAAKPGFSAASVLSNYWLDFNSCEPDELPLTCEYRIQNP
                     LIVLISLGTNDGYNPPAFKENLRTIIEITLSENRLPILMTKADNVEGDFSINQDIADL
                     AQEYDLPLWNFWAAIQDLPNHGLVEDGIHLTFYKNDFADPNAFNYAWTYRNLTALQLL
                     EKLMQATEKLQNK"
     gene            3190..3435
                     /locus_tag="PKV59_03240"
     CDS             3190..3435
                     /locus_tag="PKV59_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HQP45800.1"
                     /translation="MIEIVDILIDKIKNEILNQPNRKLKSDTPLISSGLIDSFHLVDL
                     ALMIEDTFGVHIEDYELRSDIFDTVDDLALLIDSRMS"
     gene            complement(3462..3971)
                     /locus_tag="PKV59_03245"
     CDS             complement(3462..3971)
                     /locus_tag="PKV59_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQP45801.1"
                     /translation="MGDYHSRLHQLAGSFPLVEFFALTYITDPQGNLLLIRKNENSCW
                     ELPGGAMQPDESVFECLQRTVLSVTNVFIEKGMLIQLFSDGELNYIDEDSANITPVYA
                     VFIPTSVHGSLRISREKPVEFRFFASWNIPTDKIFPPMLGAIQYYRQHFPEGIPLQPL
                     DLDQFAGYG"
     gene            complement(3973..4755)
                     /locus_tag="PKV59_03250"
                     /pseudo
     CDS             complement(3973..4755)
                     /locus_tag="PKV59_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1312 a         1063 c         1068 g         1562 t
ORIGIN      
        1 gatcggagct tttgatcatt ttcggaacgg tattgtttat cgcattatcg cgtctattca
       61 gttttgaggg aatttttacc gcatcccgtt ccccgatcct ttcggtttcg cttccatgga
      121 tcgcgggctg gtttatcctg ttttttattt tttattattt cggtggttca aaaccgaaaa
      181 aacttttttc attattcatc ggattcattc tgctgattct gattttgtta aaaacacccc
      241 ggttagcttt acaagtcagc agatttttca gaaatttagc gggtcaatct gctgagctgg
      301 cgacggcaaa cgatttacgt tggtttggat tttcttacat tgcctttcgg cttttatccg
      361 tgttaatcga tgcccggaag ggtcgaaaat gggttgcgac acccggtgat ttcctcattt
      421 atgtctgttt tcctcctgcg ttggcggccg gacccattga ccgattggat cattttgata
      481 aagaactcaa ctcagttcct catccttttc cggaagattt cagggaggga ataccgcgta
      541 ttgcgaccgg cttatttaaa aagtttattc ttgccgattc attggcactg gtcgctttat
      601 cttctcaaaa tgccgtgttg ataaagcata ccggatgggc ttgggcttct ctgtttgcat
      661 acagtcttca actttatttt gatttttcgg gttattccga tgtcgccatc ggcctcgctc
      721 atctgatcgg aatcaaatta ccggagaatt ttaatcatcc ttatctgaag ccggatatcg
      781 caaaattttg gaacagttgg catattaccc tgactcaatg gattcgttct tatacattta
      841 atccgctcgt gcgtaaatta cgaaagaaaa aagatccggc ttttccgcaa tggtttatga
      901 ttctgataac gcaatcggta acgatgggat tgatcggact ttggcatgga gtcactctta
      961 attttttcat atggggcatc tggaatgctt tgggattgtt ttttcaccag ctatatgcgg
     1021 atcatacaaa aaaccaactg atggttattt cggaagagca ctcgatcgct tttcgaatct
     1081 atacgatcgg atcgaccttg ttgacattcc tctttgtttc attgggttgg atctggtttg
     1141 tgttaccgaa tatcgattcg gcggctcttt tcttcgaaag gttattctga ggctttcatg
     1201 aaaaagattt tatcttttac tctaaaagta ttctttttcc tgatcatatt gcttgccctc
     1261 tattatctca ttcagcccga accgtttttg gggaaaatca gtctatataa tttcatattt
     1321 cccgggcggg aacgccttcc gttcggggaa aacccgcggg tggcctataa tttcagtctt
     1381 tacaatttga acgcgatgtt cgcatcccat gaaatttccg gtgtacaaga acaggataat
     1441 tctttccgcg tcatcaccat cggtgattct tccgtttggg gaacactcct gaagcccgag
     1501 gaaacacttg caggacaact ggatgggaaa aaaatcaaga ttcacggtgc cgaaagaaaa
     1561 ttggaagttt ataatctcgg ttacccaacc ctatctctgg caaaagacgt gttgatttta
     1621 cagcgcggga tggcttacaa accggatctt attttatggt ttaccactat ggagtcattt
     1681 ccgaatgaaa aacagatcga atcccctttg ctcgcaaaga attcggagga atttgctcaa
     1741 ctgttgacat cttacggtga gattccgagt tggatgccga agtcttctcc cgaaagcttt
     1801 tgggagaaga ctttgttttc ccagagacgg gcactgatgg acctgatccg tttacaactc
     1861 tacggtttca tgtgggctgc gactggaatc gatcaagatt atccggcgga ttaccctatg
     1921 gctcaagtgg attttgatcg ggacaattct tttcacggag tggacggaat ctatccaaac
     1981 gatcaaatgg catgggaatt gttgaatgtc gggatgagaa tcgccggtaa tgttccggtg
     2041 atcttaataa acgaaccgat tttaatcagt gatggtgaaa attcggatat ccgctataat
     2101 ttttactatc cccgtcaggc ttatgacgtt tggcggaaag atttggctga tcgatgtacc
     2161 gaagaaaaat ggaagtgcct ggatctttgg gatttattgt cccaatctga tttcacaaac
     2221 agcgcaatcc attataattc tgaaggagga cagaaggtag tcgatacgat tttggatgtc
     2281 ctccctgaaa gtttgcaata aaaaataaaa atgaaaaagc ctccctcgtg tagcaaaaat
     2341 ctcttttttc tgattctttc gctgcccttc tttttttcgg ttccatcttt tgcacaatcc
     2401 atcgaagatc gtcgtccttc cgattttggc agaacttggc aagatttgcc gatcgtaccg
     2461 gaattaaatg agaattcgct gcgaattttg aaagccggaa tcgctgcggg tcataatccg
     2521 catgcttttt caaaggtcgg tgattgcgat accagtacgg actggtattt ggcggatttt
     2581 gataaagatt cccgatacta taatttgggc gaatatagag atcgatttga acctgtttta
     2641 tcttattaca agggatcatt ttctcatcga agtttagccg caaaacccgg tttttcggct
     2701 gcttcggtat tgtccaatta ttggctggat tttaattctt gcgagcccga tgagcttccg
     2761 ctcacctgcg aatatcgcat tcagaatcct ctgattgtat tgatctcgtt gggtacaaat
     2821 gacggatata atccgccggc attcaaagaa aatttaagga caatcattga aatcacttta
     2881 tcggaaaatc ggttgccgat tctgatgacc aaagccgata atgtcgaggg agatttttcc
     2941 atcaatcaag atatcgctga tttggcacag gaatatgatt tgccgctctg gaatttttgg
     3001 gctgccattc aagatttacc gaatcacgga cttgtagagg atggtattca tttaactttt
     3061 tataagaatg attttgccga tccgaatgca tttaattatg catggaccta tcgtaatttg
     3121 acagcattgc aactgcttga aaagctgatg caggcgacag agaagttgca aaataaataa
     3181 gaggaataaa tgattgagat agttgatata cttattgaca agatcaaaaa cgagattctg
     3241 aatcaaccca atcgaaaatt aaaatcggat acaccattaa tcagcagcgg tctgattgat
     3301 tcatttcatt tagtcgattt ggcgttgatg atagaagata cattcggtgt tcatatcgag
     3361 gattacgaac tgcgatccga tatatttgac accgtggatg atcttgcgtt gctgattgat
     3421 tcccgcatgt cttaatccat taattttctg tttcaggcga atcatccgta acccgcaaat
     3481 tggtccagat caagcggttg aagcgggatt ccttctggaa aatgttgccg gtaatattga
     3541 atcgctccga gcattggcgg aaagatttta tcggtgggaa tattccaact ggcaaagaaa
     3601 cgaaattcaa caggtttctc acgagaaatg cggagcgaac cgtgcacact ggtcggaata
     3661 aagaccgcat aaacgggtgt aatatttgcg gaatcttcat caatataatt taattcaccg
     3721 tctgaaaaaa gttgaatcaa cattcctttt tcgataaata cgttcgtaac ggaaaggacg
     3781 gttcgttgaa gacattcgaa aacactttca tccggctgca tcgccccacc cggtaattcc
     3841 caacagctgt tttcgttttt tcgaattaat aacaaattcc cttgcggatc ggtaatataa
     3901 gtcaacgcaa aaaattccac caaaggaaag ctgcctgcta attgatggag gcgagaatga
     3961 taatcaccca tattatgaga aatattccct taaaactcgg ctccggaccg gatgccgtaa
     4021 tttccttagc gccttggcct caatttggcg aacgcgttcg cgggttatat taaatttctt
     4081 gctcacttct tccagagtct gctcttctcc gtcgatcaaa ccgaatcggt actccaagac
     4141 ttgacgctct cgttccgtca aaatgtccag cgttttcatc acctgatctt ttaaaatttc
     4201 cttggcagcc gcatccatcg gctccaacgc atcttcatct tcaagaaaat ctccgaattg
     4261 accgctttct tcatctccga tcgggtgttc cagcgatatg ggctcttcag cggattgcag
     4321 cagattctga actttgttta tcgctatcga taattttttc tgaagatccg ggctcagcgg
     4381 tttgttttgc tctcgacagg tccttattct ttcgctgtcc tcgggatcca aaaaatgagc
     4441 ttcgatggca agttcttcca tacttggatt tcggccgagc tcttgaacca actttcgctg
     4501 gatcttcatg accttgttta cggcatcaac catatgtgcg gggatacgaa taatgcgagc
     4561 ctgttctgcg atgtaacggc tgattgcttg ccgaatccac caagtggaat aagtgctgaa
     4621 tttgaaccct ctgcgcggat caaatttatg gacggcgcgc aacaatccta tgtttccttc
     4681 ttgaattaaa tccaaaaacg aaattcctct tccgagatat cgttttgcaa tactgacaac
     4741 cagacgtaaa tttgcgcgga tcaaaatctg atttgccact tcggattggg tctgaacctt
     4801 gttaatatgg gtaatcatat caaaggaaac cggcaacatt tcgattagag ttttctgatc
     4861 gggaagtaca ttgccgtttg ccttcaagta ttcaagaagt ttatcctatg ctttcaggca
     4921 gcagataaag acccagaaaa atatcataaa ccggatcgac gaccttatac caatccggat
     4981 cggaacccca tcttccgtta tccag
//
LOCUS       DAOMIV010000090         4907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_17499_length_4907_cov_3.953421, whole genome shotgun sequence.
ACCESSION   DAOMIV010000090 DAOMIV010000000
VERSION     DAOMIV010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17499_length_4907_cov_3.953421"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /gene="mvk"
                     /locus_tag="PKV59_03255"
     CDS             complement(<1..840)
                     /gene="mvk"
                     /locus_tag="PKV59_03255"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HQP45802.1"
                     /translation="MSEAPAKVILFGEHAVVYGEPGIAIPISALKTSAFFENNIHNAF
                     SIQSAPLGMDLNYLELADDHPLKKLIILLMNEFGITSLPEKKLRIESAIPIASGLGSG
                     AACSVAVIRAFAEEYQKSISTQRISEIAFEIEKIYHGTPSGLDNTVISFGVPIYFIKN
                     QPFQFIQIKKPLNLLIVDTEIESNTAEVVKDIRDHYATFSPLIHEIGDISRTAKTALE
                     DGDIKLIGELMNKNHDLLKRLTVSSPELDYLYEIAIEQGAIGAKLTGSGRGGNIIALV
                     EEED"
     gene            complement(850..1725)
                     /locus_tag="PKV59_03260"
     CDS             complement(850..1725)
                     /locus_tag="PKV59_03260"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HQP45803.1"
                     /translation="MDRIKPYLKAMRINQWTKNAFIFAPLIFDGKLTHFPSFLRILIG
                     TIIFSVLYSGIYVFNDIVDIEADRLHPRKKNRPIASGTISIKNARIFSIALIASALLC
                     GGFLQSEFLLILVLVLFINIIYTFFLKRIPIIDVIAIGVLFLLRLVAGVVLITVKMFS
                     PWLYLVTFMLSLYLGFGKRRSELAALTGNSSQTRPVLNGYSLELLDQLITIVSSVTIM
                     AYSLYTFSGPTLPGNNFMMITIPIVIYGIFRYHYLIQIKHAGGAPEEVLLRDRPLQAT
                     ILIYLLAVCIGLYLY"
     gene            1909..2700
                     /locus_tag="PKV59_03265"
     CDS             1909..2700
                     /locus_tag="PKV59_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HQP45804.1"
                     /translation="MKAEKKRLDVLVQENGLAESRSLAQKLIMAGKVRVNDNMVIKPS
                     TLCSVDDRISLDAGPRFVSRGGEKLSGALEAFGADLPNGKICADVGASTGGFTDCLLQ
                     YGAKKVYAIDVGYGILHWKIRNDPRVVSMEKTNARDLKSLPEPVELVTVDASFISLQI
                     LLPVIRNWFGDSGGTVISLIKPQFEAGKKEAAKGAGVIRDPEIHKKVLMETLGKAEEM
                     GYGIYGLIRSPLKGPKGNTEFLVWLQTVPNEFNIKEAVQNTVENS"
     gene            complement(2786..3100)
                     /locus_tag="PKV59_03270"
     CDS             complement(2786..3100)
                     /locus_tag="PKV59_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HQP45805.1"
                     /translation="MYESKSKSPETDELCRAILSLKSVEECYRFFDDIFTIREIKDIS
                     QRLQVAKLLRSGAIFNEIVKKTGASTATIARVNRCLNYGADGYKMILNRLDEEEKKEI
                     KE"
     gene            complement(3195..3346)
                     /locus_tag="PKV59_03275"
                     /pseudo
     CDS             complement(3195..3346)
                     /locus_tag="PKV59_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016078581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="polar amino acid ABC transporter ATP-binding
                     protein"
     assembly_gap    3347..3446
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3447..3751)
                     /locus_tag="PKV59_03280"
     CDS             complement(<3447..3751)
                     /locus_tag="PKV59_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP45806.1"
                     /translation="MEIFSINDVRKSFSTQTDVLCGMSLSVQKGEVVAIIGPSGSGKS
                     TLLRCATLLETMDSGELVYEGIKAAWNGPDGNVVYAPEETLKKIKSKFGLVFQNFNL"
     gene            complement(3760..4413)
                     /locus_tag="PKV59_03285"
     CDS             complement(3760..4413)
                     /locus_tag="PKV59_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HQP45807.1"
                     /translation="MGLGLMLNKLSEGMATSVLIFVLTLLFSLPLGLLVALMRMSRHK
                     VLQNLMRIYISVMRGTPLMLQLLVVYFGPYYIFGIKLSPDYRFWAVIIAFVINYAAYF
                     AEIYRGGIASVPSGQYEAAKVLGYSSSQTFLKIIFPQMIKRIIPPITNEVITLIKDTS
                     LAFAVAVTEMFTVAKALASAEKTMTAYVFAGVFYYVFNLIVAFVMENIEKRLNYYRL"
     gene            complement(4506..>4907)
                     /locus_tag="PKV59_03290"
     CDS             complement(4506..>4907)
                     /locus_tag="PKV59_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQP45808.1"
                     /translation="DKVVAVQADSAGLYAIQDEKNKELLDSFKELLVVPQYNEAFMDL
                     EAGAVDAIILDIDVAKFQIKGKESKYRILDEYLQKEEYGVGFKLGNTELRDKVQEALN
                     EMAKNGTFAKLSEKYFGYDSCIMAACLANEK"
BASE COUNT         1460 a          999 c          982 g         1366 t
ORIGIN      
        1 atcttcttct tcaaccaatg cgatgatgtt gccgcctcgt ccagaaccgg taagtttggc
       61 gccgattgcc ccttgctcta tagcgatttc gtataggtaa tcaagctccg gtgacgaaac
      121 ggttaatcgc ttcagcaaat catggttttt attcattaat tcaccgatca gcttgatatc
      181 accatcctcc aaagctgttt ttgccgttcg agaaatgtct ccgatttcat gaatcagagg
      241 cgaaaaggtc gcgtaatgat cccgaatatc tttcacaact tccgccgtgt tgctttcaat
      301 ttccgtatct acaatcaaga ggtttagcgg tttcttgatt tggatgaatt gaaacggttg
      361 gtttttaata aaataaatcg gtactccgaa actgatcacc gtattatcca atcccgacgg
      421 ggttccgtga taaatttttt caatttcaaa agcaatttcc gaaatccgtt gggtgcttat
      481 cgatttttgg tattcctcgg caaaggctcg aattacagca acagaacagg ctgcacccga
      541 tcccaaaccg gacgcaatcg gaatagccga ttcgatccgt aatttctttt ccggtaacga
      601 ggtaattcca aactcattca tcagcagaat gatcaatttt ttcaacggat gatcgtctgc
      661 aagttcaaga taattcaaat ccatcccgag cggtgcggat tgaatcgaaa aagcattatg
      721 gatattgttt tcgaaaaaag cgctcgtttt aagcgctgaa atagggatag caatacccgg
      781 ttccccgtaa acaacggcat gttcaccgaa taagattact ttagccggtg cctcactcat
      841 attaacctat taataaagat aaagtccgat gcaaacagct aagagataaa tcaaaattgt
      901 cgcttgtaaa ggtcgatctc ttaagagaac ttcttcggga gctccgcccg catgtttgat
      961 ttggattaag tagtgatatc tgaatatccc ataaatgaca atcggaatcg tgatcatcat
     1021 aaaattattt ccgggcaaag taggtccgga aaatgtatat aacgaatatg ccataatcgt
     1081 gacgcttgaa acaattgtta ttaattgatc caataattcg aggctataac cgtttaagac
     1141 cggacgagtt tgtgaagaat taccggtaag tgccgccaat tcggaacgac gtttcccgaa
     1201 gcccaaatac agagaaagca taaaagtgac caagtataac caaggagaaa acatcttcac
     1261 cgtaattaat acaacgcccg cgaccagtct caaaagaaat aagactccga tggcaatcac
     1321 atcgataatc gggattcgtt tcaagaagaa agtatagatg atattaatga agaggaccag
     1381 aactaagatc aaaaggaatt ccgattggag gaaaccgccg cataaaagtg ccgatgctat
     1441 caaagcgatt gaaaagatcc gagcattctt aattgatatt gttccggatg cgatcgggcg
     1501 attcttttta cgcggatgaa gacggtccgc ttcaatatcg actatatcgt tgaaaacata
     1561 aattccgctg tagagaacgc tgaaaatgat cgttccgatc agaattcgca gaaatgaagg
     1621 aaaatgggtt aatttcccgt caaaaatcaa cggagcaaaa ataaatgcat tcttggtcca
     1681 ttgattaata cgcatagctt tcaaataagg ctttattcga tccatagcat gtatattata
     1741 atcggtgaga catgaacgat gacattttgt gaaattgaga tatggccgag cgaataattt
     1801 cggtgtaaaa catattctat taaaaatcgc gccaaaaaat gatcgtttct gaaatttgtt
     1861 tattgagctt tatcacttta tggcttataa aatcaatgga gtctccccgt gaaagctgaa
     1921 aagaagaggt tggatgtttt ggttcaggag aacggcttgg cggaaagccg gtctttagcc
     1981 caaaaattga tcatggccgg aaaagttcga gtgaacgata atatggttat caaaccgtca
     2041 actctctgtt cggtcgatga tcggatctcg cttgacgccg gaccgcgttt tgtttcacgt
     2101 gggggagaga aattatcagg tgccttagaa gctttcggtg cagatctccc caacggaaaa
     2161 atttgcgccg atgtcggagc atccaccggc ggatttacgg attgtctctt gcaatatggg
     2221 gcaaagaaag tctatgcgat tgatgtcgga tacggaatct tgcattggaa gatccgaaat
     2281 gatccgagag ttgtctccat ggaaaagacg aatgctcgtg atttgaagag cttaccggaa
     2341 cccgttgaac tggtaaccgt tgatgcgtcc tttatttctt tacaaattct tttgcccgtt
     2401 atccggaatt ggtttggcga ctccggcgga acggtaatca gtctgatcaa accgcaattt
     2461 gaagccggga aaaaggaagc cgcaaagggt gccggtgtga ttcgtgaccc ggaaattcat
     2521 aaaaaagttt taatggaaac gctcggaaaa gcggaagaaa tgggatacgg aatatatgga
     2581 ttgatccgtt ctcctttaaa aggtccgaaa ggaaatacgg aatttttagt ttggctgcaa
     2641 actgtaccca atgaatttaa tattaaagaa gcggttcaaa atacggtgga aaacagttaa
     2701 tagtagagag caaaaaaaat ctctcctgta aagagtaaaa aaggcttcta tacgataact
     2761 gccggaattt ttttcaaagg agaaattatt ctttgatttc ttttttctcc tcttcatcca
     2821 aacgattcag aatcatttta tatccgtccg caccataatt caggcagcgg tttacccggg
     2881 caatggttgc cgtggatgcc ccggtttttt ttacaatttc attgaagata gctcctgatc
     2941 ggagcagttt ggcaacctgt aacctttgag aaatatcttt tatttctcgg atagtaaaaa
     3001 tatcatcgaa aaagcgataa cactcttcga ctgatttcaa tgaaagaatg gctctacaaa
     3061 gctcatccgt ttcggggctt ttcgatttac tttcgtacat aatgaaacct cgctgtttgt
     3121 taaagattta atttgcgaag aaaagctttc gtccgttcgt tttcaggatt tcttataatt
     3181 ttatctgaat ttccttcttc aatgataaca ccggcgtcca taaaaacaac ccgattggca
     3241 acgatccgcg caaattcgat ttcatgggtt acgatcacca ttgtcatttt ttcttcggcg
     3301 aggtctcgaa tgacatgcaa aatttcaccc gtcaattcgg gatccannnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa gattgaagtt ttgaaaaacc aaaccgaatt
     3481 tactctttat ttttttaagg gtttcttccg gagcataaac gacatttccg tcgggtccgt
     3541 tccatgcggc ttttatccct tcgtatacca gttcacccga atccatcgtt tccaacaaag
     3601 tcgcacagcg taacagcgtt gatttccccg atccggaagg cccgatgatg gcgactactt
     3661 ctcctttctg aacggataaa ctcattccgc ataaaacatc ggtttgggtc gaaaaacttt
     3721 tcctgacatc attgatactg aatatttcca ttcaatcgct cacaatctat agtagtttag
     3781 tcttttttca atattttcca taacaaatgc aacaatcaga ttaaaaacgt aataaaaaac
     3841 tcccgcaaac acataagcgg tcatcgtctt ttcggctgac gcaagcgctt ttgcgactgt
     3901 aaacatttcc gttaccgcga ccgcaaatgc taaagaggtg tccttgatca acgtaatcac
     3961 ttcgttcgta atcggtggga taattcgctt gatcatctga ggaaaaatga ttttcaagaa
     4021 agtctgtgaa gacgaatatc ccaagacttt cgcggcttca tattggccgg acggcacaga
     4081 tgcgatgccg cctcgataga tttccgcgaa atacgcggca tagttgatga caaaagcgat
     4141 aattacagcc caaaaacggt aatcgggaga aagtttgatc ccgaaaatat aataaggccc
     4201 gaaataaacg accaaaagtt gcaacattaa tggcgttcca cgcataacgg agatgtaaat
     4261 tcgcatcaga ttctgtaaga ctttgtgacg gctcatcctc atgagagcca ctaaaaggcc
     4321 gagcggaagg ctgaacaaca aagtcaggac aaatatcagg accgaagttg ccatcccttc
     4381 ggataactta ttgagcatta accccaaacc cataaaaatt ctccccgaaa tatcgattcc
     4441 aaatattgag ggcacttccg attaaaagtg ccctcaataa aggaagagat gtaattgtca
     4501 atttcttatt tttcatttgc caggcaggca gccataatgc aggaatcata accgaaatac
     4561 ttttcggata attttgcaaa ggtaccgttt ttagccattt cattcagtgc ttcttggact
     4621 ttgtctctga gttcggtatt tccgagcttg aatccgacgc cgtattcttc tttttggaga
     4681 tattcatcca agattcgata tttggattct ttgcctttaa tttgaaactt cgcaacatca
     4741 atatccaaaa taatggcgtc aacggcacct gcttcaaggt ccataaaagc ttcattatat
     4801 tgagggacga ctaaaagttc tttaaatgag tctaacagct ctttattctt ctcatcctga
     4861 atggcataaa gtccggctga atccgcttga actgcgacaa ctttatc
//
LOCUS       DAOMIV010000091         4907 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_17500_length_4907_cov_3.378195, whole genome shotgun sequence.
ACCESSION   DAOMIV010000091 DAOMIV010000000
VERSION     DAOMIV010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17500_length_4907_cov_3.378195"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PKV59_03295"
     CDS             <1..375
                     /locus_tag="PKV59_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQP45809.1"
                     /translation="KFQGLRQAVDIPGKTLEKLYTCFSMHIQTMQRYRALPNLVLADT
                     LWDDVPELRERMYASHHKQQQMTAELIDNPAFAQVLKTYCPMGRAGKEGELDGAVVYL
                     ASDASSFTTGQILNVDGGWTAI"
     gene            592..1287
                     /locus_tag="PKV59_03300"
     CDS             592..1287
                     /locus_tag="PKV59_03300"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HQP45810.1"
                     /translation="MPEKKSNAKIKITKNGPYLVSGNIPIHEKIIVPTGHTYKLVEGR
                     ELPQAENYALCRCGKSKNPPFCDGSHLIEPFNGKETASREPYTDRSELLEGPGVDLLD
                     DGRCAFARFCHRKNGTTWDYVQNSNTEEAIREAIQAAGECPSGRLTAVTKEGELSEPD
                     YDPEIDIVEDPERGVSAGIFVKGKIPIESEDGEIYELRNRVALCRCGHSRNKPFCDAS
                     HVPFGFSDGHVPE"
     gene            1460..2797
                     /locus_tag="PKV59_03305"
     CDS             1460..2797
                     /locus_tag="PKV59_03305"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HQP45811.1"
                     /translation="MIFLLRKTMDLLIAFLVLIVSVLFSLFQKFSLLIPLGIGLICFI
                     SVGLYRHYPLRSLFKMALEGAKNSLVVIRVLVLIGCLTALWRACGTISFFVVFGVKLI
                     TPHIFILVAFLLSSLVSYAMGTSFGVAGTVGVILMSIARAGGVNEYIAAGAILSGVYF
                     GDRTAPTSSCANLVASLTKTDLYDNVKRMLKSAALPFVICIFLYAGLSWKNPLSSADT
                     SLVSVLENNFSLSVWTLIPAVILLILPLFRIPVRIAISISILFSFAVSMGSQQMGFED
                     TFHAMLFGYKPSDPTLTGILSGGGLIPMLTTILIVALSSTYSGIFNGTNMISGIREKL
                     FDLSEKIGRFPTVVATSILSCAVFCNQTMGIIFTHEMVGKIYQEKKIDRSTFMLDIAN
                     STTLIAVLIPWSIASSVPLTWMGVNFKALPYAFLLYLIPLFYGIQEQHKSKQH"
     gene            2913..3338
                     /locus_tag="PKV59_03310"
     CDS             2913..3338
                     /locus_tag="PKV59_03310"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HQP45812.1"
                     /translation="MPWTTKNYPNSWKNFDPLLRKKAIDIANAMLADGYKEEDIIPIA
                     TSQAQKWYENATPAELKELEEKDVTKHTKPKGPSGARLMEKDVIVRYQPEQKQWEVIS
                     DGALQADSLHHTKKEAVIRAREIAGKRGTRVIEKKKSES"
     gene            3644..>4907
                     /locus_tag="PKV59_03315"
     CDS             3644..>4907
                     /locus_tag="PKV59_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQP45813.1"
                     /translation="MALAVFLFVLTYILLLALPKYRAYVALVSAILFISTGIISPVKA
                     VEGIDWNVLMMITGTMGTVYLFCQSRMPEKMADVILERMPDVKWVIVSLALFAGIISA
                     FVDNVATVLMVAPIALTVAKRLKISPVPIIIAIAVSSNLQGAATLVGDTTAIMMGSQA
                     GMDFIDFFFFHGRPSMFWFTEVGAVVSALILLWQFRDKNEKVTDVLNTEVNDYVPSIL
                     LFGTVLLLIVASFINPKPKITNGLICMSIMLIGIVFEGIKNHGLADSFRETFGSIDFF
                     TLLLLASLFMVIAGIKEAGVIDSLAALFVRLSGNSLILIYSLILWGSVLISAFIDNIP
                     YVATMLPVVAGIASYMQIDPTLLYFGLLSGATLGGNLTPIGASANITGIGILRKNGYE
                     PSTQDFMKISVPFTLSAVTSAYILIWVFW"
BASE COUNT         1305 a         1064 c         1101 g         1437 t
ORIGIN      
        1 aaatttcagg gactacgcca ggccgtagac attccgggca aaacgcttga aaaactgtac
       61 acctgctttt ccatgcatat ccagaccatg caacgctaca gggcgctgcc caacctggtc
      121 ctggccgata ccctctggga cgatgtgccg gaactgcgcg agcgcatgta cgcctcgcac
      181 cacaagcagc aacaaatgac cgccgaactg atcgataacc cggcgttcgc tcaggtactg
      241 aagacctatt gcccgatggg cagagccgga aaagaaggtg aattggacgg agcggtagtt
      301 tacctggcat cggatgcttc cagcttcaca accggtcaaa tcctaaatgt cgacggcggt
      361 tggacggcta tctaaacaga tttccgaaag gaaacaaatc tatcggataa aaaagaagcg
      421 gttgcctaaa aagcaactgc tttttttcat ccgaaactca gagaacagcg tttcgatgcc
      481 acatcggata atatctggaa ttgtctttaa tccggctaag actttacgcg gcattataat
      541 cagaagcgtt caattaacgt tttgagatgt gaatgaaaag gatgatttta tatgccggaa
      601 aagaaatcga acgccaaaat taaaataaca aagaacggtc cctatcttgt atcggggaac
      661 atcccgatcc atgagaaaat cattgttccc accgggcaca catacaaatt agtcgaggga
      721 agagagttgc cgcaggcaga aaattacgcg ctttgccgtt gcgggaaatc aaaaaatcca
      781 cctttctgtg acggttctca tctgatcgag ccttttaatg gaaaagagac cgcttcccga
      841 gaaccttata cggatcgttc cgaattactc gaaggaccgg gagttgatct gctggatgac
      901 ggcagatgtg cctttgcccg attttgtcat agaaaaaacg gcacgacctg ggattatgta
      961 cagaattcaa atacggaaga ggcaatcaga gaagcgattc aggccgccgg tgaatgccct
     1021 tccggaagat taaccgccgt aaccaaagaa ggcgagttgt cggagccgga ttatgatccg
     1081 gaaatcgata tcgttgaaga cccggaaagg ggtgtgagtg ccggcatctt tgtgaaagga
     1141 aaaatcccca tcgaatccga agacggtgaa atttatgagc taaggaaccg agttgcctta
     1201 tgcagatgcg gacattctcg aaacaaacct ttctgcgatg cctcccatgt tcctttcgga
     1261 ttttcagatg ggcatgttcc ggaatagtct tttggaagcg aacgagaaat gcattcgata
     1321 aaaaaagtat ccggaaaaat ccttttactt gattttcccg tcataagttc cataaaacgg
     1381 gttcgatgat cgatacattt ctgagtcgat acggaaggaa acgagtcaat cagccgatat
     1441 tcgtcaaccg acccggcgat tgattttttt attaaggaaa acaatggatc tgttaatcgc
     1501 ttttcttgtg ctgatcgttt ccgttttatt cagtctgttt caaaaatttt ccttactcat
     1561 tccattggga atcggtttga tctgcttcat ttcggtcggt ctttatcgcc actatccgct
     1621 tcgttcttta tttaaaatgg ctttggaggg tgcaaaaaac tcccttgtcg tcatccgggt
     1681 tttggtttta atcggttgcc tgacagcctt gtggcgagct tgtggaacga tttctttctt
     1741 tgtggttttc ggcgtgaagc tcattacgcc ccatattttc attttggtag ccttcttgct
     1801 ttcttccctt gtttcttatg ccatgggaac cagcttcggc gtagccggca cggtgggagt
     1861 gattttaatg tcaatcgccc gagctggggg tgtcaacgaa tatattgccg ccggagcgat
     1921 tttatccggt gtgtatttcg gagaccgaac ggcgccgaca tcctcttgcg ccaatttggt
     1981 tgcttcgctt acgaagaccg atctgtacga taatgtgaag cgcatgctga aatccgccgc
     2041 gcttccgttc gtgatttgta tctttcttta cgccggttta tcttggaaga atccgctttc
     2101 aagcgcggat acatccttgg tcagcgtttt ggaaaacaat ttttcgctct cggtttggac
     2161 ccttatcccg gctgttatct tattaatttt gccattgttt cgcattccgg taagaatcgc
     2221 catttcaatc agtattttat tttccttcgc ggtcagtatg ggaagtcagc aaatggggtt
     2281 tgaggatact tttcatgcga tgctgttcgg ttacaagccc tcagacccca ctttgaccgg
     2341 tattttatcc ggaggcggat tgattccgat gcttaccacc attcttatcg ttgcgttatc
     2401 gagcacatat tctggcattt ttaatgggac gaatatgatt tcaggaattc gggagaaact
     2461 attcgatctc tccgaaaaaa tcggccgttt ccccacggtg gtagcgacga gcattctcag
     2521 ctgcgcagtt ttttgtaatc aaacgatggg cattatattc acacatgaaa tggtcggaaa
     2581 aatctatcaa gagaaaaaaa ttgatcgaag cacttttatg ttggatatcg ccaattcaac
     2641 cacgctgatt gccgtactga ttccttggtc aatcgcttct tcggttccgt tgacttggat
     2701 gggtgtgaat ttcaaagcgt taccttatgc ttttctgctt tatttaatcc ccttattcta
     2761 tggaatacag gaacaacata aatcaaaaca acattaaaca cttcaaaata atattcttta
     2821 cattcttttg tcgataaatc caggtatttt tttgattaca attaaaaaga actcccttta
     2881 taaaaaagga gttgtcaaaa aggaggtctg ttatgccttg gacaacaaaa aattatccga
     2941 atagttggaa aaactttgat ccgctgttgc ggaaaaaagc gatcgatatc gcaaatgcca
     3001 tgttagctga cggctataaa gaagaggaca tcatccccat cgcgaccagc caggcgcaaa
     3061 aatggtatga aaatgccact cccgctgaac tgaaagaatt agaagagaaa gatgtcacca
     3121 aacataccaa accgaagggt ccttccggtg cacgcctcat ggaaaaagac gtaattgtcc
     3181 gatatcaacc cgagcaaaaa caatgggaag tcatttccga tggcgcactg caggcggata
     3241 gtctgcatca taccaaaaag gaagccgtca tccgtgcccg agaaatcgcc ggaaagcgtg
     3301 gcactcgtgt gattgagaaa aagaaatccg aaagctgaaa gcagccggaa aataaattct
     3361 ttttcaccgt aggaaccggc aggctgtcga ttgacagcct gcctatttaa atgtttttgt
     3421 cggagaagaa aatgatttat tctttttatg aataaatcat ttttttagaa tgattctcac
     3481 cggtaaaatc gatccgttat tgaaagttat ttgaagtggt ataattcgcc cgcgaatgga
     3541 atatttaaga ttttatttta tatcgcctct tcggaaatca ggtaatttta ttccccaatt
     3601 tgatatttgc tcaatcgata aagattaata aaaaaaggaa aatatggcac ttgctgtttt
     3661 cttatttgta cttacataca ttcttctttt ggcacttccg aaatatcgag cttatgtggc
     3721 attggtttcc gccatccttt tcatcagcac cggcatcatc tctccggtga aagctgtcga
     3781 aggcattgat tggaatgtgc tcatgatgat taccggtacg atgggtacgg tttatctttt
     3841 ttgccaatcc cgaatgcccg aaaaaatggc ggatgttatt ttagagagaa tgccggatgt
     3901 caaatgggtc attgtcagtt tggctttgtt cgccggaatt atctccgctt tcgtggataa
     3961 tgttgccacc gttttgatgg ttgctccgat cgcattgacc gttgcaaaac gactgaaaat
     4021 ttcaccggtt ccgatcatca tcgcaattgc cgtttcttct aaccttcaag gggcggcgac
     4081 tctcgtcggg gataccacgg cgattatgat gggcagtcaa gccggtatgg attttattga
     4141 tttcttcttc ttccatggtc gacccagcat gttttggttc accgaagtcg gagcagttgt
     4201 ttccgcactc atcttgcttt ggcaatttcg ggataaaaac gaaaaagtca ccgatgtgtt
     4261 gaataccgaa gtgaatgatt atgtgccttc gattttgtta tttgggacgg tgttattgtt
     4321 gattgtcgcc tcatttataa atcccaagcc gaaaataacc aatggcttga tttgtatgag
     4381 cattatgttg atcggcattg tattcgaagg aattaaaaat catggattag ccgattcttt
     4441 ccgagaaact ttcggatcga ttgatttttt cacattacta cttttggcaa gcctctttat
     4501 ggtaattgcg ggtattaagg aagccggcgt aatcgacagt ttggcggcac tttttgtcag
     4561 gttatccgga aacagcctga ttcttatcta ttctctgatt ttatggggct cggtgttgat
     4621 atccgcgttc atcgataaca tcccttatgt cgcaacaatg ctccctgtcg tagccgggat
     4681 tgcttcttat atgcaaatag atcccactct cctctatttc ggacttcttt ccggcgccac
     4741 gctgggcggt aatttaacgc ccatcggcgc atctgcgaat ataaccggaa tcgggattct
     4801 gcgaaaaaac ggctatgaac cgtccaccca agattttatg aaaatcagtg ttccttttac
     4861 tttatcggca gtcacttcag cgtatattct gatttgggtt ttctgga
//
LOCUS       DAOMIV010000092         4904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_17506_length_4904_cov_4.434523, whole genome shotgun sequence.
ACCESSION   DAOMIV010000092 DAOMIV010000000
VERSION     DAOMIV010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17506_length_4904_cov_4.434523"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1503
                     /locus_tag="PKV59_03320"
     CDS             208..1503
                     /locus_tag="PKV59_03320"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQP45814.1"
                     /translation="MSDEIVQTKLENGLTVLLKEIHSAPIISHWIWYRVGSRNEVPGK
                     TGISHWVEHMQFKGTAKYPAGVLDQAISRGGGIWNAFTYLDWTAFFSTMPKDKIALPI
                     DLEADRMQNSLFLPEEVESERTVILSEREGSENEPLFRLNEAIDKAAFDRHPYRFEII
                     GEKSDLLTLTRDDLYTHYRQFYAPGNAVLTMAGDFDANEMLDRLAAVYQSVSARPIPT
                     VTIQPEKAIPEERHVELSGPGDTTYLRIVWRAPAGRDPDIFPLAVLDSILTGPSSLNM
                     FGSGSISNKTSRLYQPLIDRQIAVAFSGDYNATIDPYLYMMTVIMHPDHDPQEAVRII
                     DEEISRIQSGDISEQELKKAVKQARALFAYGSENITNQAYWMGYTEMFSSYSWFTEFV
                     SSLNKVTVSDVAKSASTYLNSAHRVIGIYRPYPEESEES"
     gene            1500..2789
                     /locus_tag="PKV59_03325"
     CDS             1500..2789
                     /locus_tag="PKV59_03325"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQP45815.1"
                     /translation="MKNIQSIPGSDDILRIELPNQIVLLARENSLSPSVVISGYLPGG
                     SVLDPVDRSGLAIFLSLALMRGTETNSFEQIFRKLEDNGASLGFNAGFHSFSFAGQSL
                     AEDLPVMLDLLNDILRHPIFPENHLTQIRDQMLTALAIRSQDTAEMANVAFDQALFGS
                     HPYGRDKLGTPESVQSVSRSDLVRHHAQYFKPQGMVISIVGGIEPEKAADLVRKYFSD
                     WEAGPQAIPNLPDFPKVTKPEKGSATHIEIPEKSQLDLVIGSIAPERKSPQYQPARLG
                     NCILGEFGMMGRIGRVVRDENGLAYYAGSDVNCLPLGGSWEISAGINPENLDRTVDLI
                     RSEIQKFVSTPVEPNELSDVQSYYLGRMPLQLESNAGIANELLSIERYQLGLDYLRRY
                     GDIIRAVTREQILETAATYLDPDRLTLATAGTLRGKA"
     gene            2786..3157
                     /gene="acpS"
                     /locus_tag="PKV59_03330"
     CDS             2786..3157
                     /gene="acpS"
                     /locus_tag="PKV59_03330"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQP45816.1"
                     /translation="MTTILRSGIDLIEIERIEKLKPEIKARFLQRVYTSAELDLCGDR
                     DERLAGRFACKEAVSKALGTGIGTVHWQEIEILTDDQQMPILYLHGNAAALAKSLGLS
                     IWSVSISHVKNYAVAIAIASD"
     gene            3207..3869
                     /locus_tag="PKV59_03335"
     CDS             3207..3869
                     /locus_tag="PKV59_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQP45817.1"
                     /translation="MTIKILALSDVETGLIYSERSDEYFPAVDLIVSCGDLPMSYLDY
                     AATTLNAPLYFVLGNHNSLIPVSKTSNYPYNSSGYNLHCSNYFYEKRLLIAGVEGSVK
                     YNNGPQQYSQSEMWTNVFSLVPALLRNKLLHGRYLDLFVSHAPPWQIHDQQDLPHNGI
                     RAFRWLDKVFKPKIHLHGHIHVYRSDTVRETVYYNTKVINCYGYREIFFDEPTDNLPK
                     ES"
     gene            3934..>4904
                     /locus_tag="PKV59_03340"
     CDS             3934..>4904
                     /locus_tag="PKV59_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45818.1"
                     /translation="MTDKLLVLFGIGCIFVFLFASCSILTGLQEVDPQSATLTALVTP
                     TSVQPTLDLKGNKVLEDRSCLIFDSPSISTFADAEDGGIFAWAGNKNLLAFVVPENRY
                     WAWFSGDAEIVDFEKDYKNPTIMNTTGLKVFGDFAFNPSATKIAFVVLRTSEKIYTVV
                     VATLSSNLQTTIDLFTDTSARTDNYSSSKSVIQWENDDEVTVTTSCGIDCDQIYRINT
                     VTGTTRLIDEVRKKGHTGRDFPQNVPEYDEREYPAMNQPNWSPDLSMVFYTDDQNKTW
                     ILNDKTKEQFELPITADKVLQTAWSHDAQFIAIRFAENLSVYKVNCK"
BASE COUNT         1378 a         1100 c         1146 g         1280 t
ORIGIN      
        1 catcaaccga ttctgtgccg gaagttcaaa tcgaagccgt cagccaacaa taatccgaaa
       61 atcaatttcc gagattcggg atatcataaa aaaagtctgc ggaacctaca aaaaggttcg
      121 cagactttta ttttgtttta gaatagaatg gttaatttaa gtcttaagtc ggacgacgga
      181 aaacccaaag gaggaaaccg caatttcatg agcgatgaaa tcgtgcaaac aaaattggaa
      241 aacggtctga ccgttttatt aaaagaaatt cacagtgctc cgattatcag ccattggatc
      301 tggtatcgtg tcggatcgcg aaacgaagtc cccgggaaaa cgggaatctc tcattgggtg
      361 gagcatatgc agtttaaagg gaccgccaaa tatccggcag gtgtgctgga ccaggcaatt
      421 tcgcgcggcg gaggcatctg gaatgcattc acctacctgg attggacggc tttcttttca
      481 accatgccga aggataaaat cgctttaccg attgatcttg aagccgatcg tatgcaaaac
      541 agcctgttcc tgccggaaga agtggaatcg gaacgcaccg ttatcctttc cgaacgcgaa
      601 ggatcggaaa atgaaccgct tttccgatta aatgaagcga tagacaaggc tgcatttgat
      661 cgtcatcctt atcgttttga gatcatcgga gaaaaatccg atttgttaac tcttactcgg
      721 gatgatcttt atacgcatta ccggcaattt tatgctccgg ggaatgcggt cctgacaatg
      781 gccggcgatt tcgatgcgaa cgaaatgttg gatcgattgg ccgccgtata tcaatccgtt
      841 tcagccagac ccattcccac ggtgacgatc caacccgaaa aagccattcc cgaggaacgg
      901 cacgtcgaac tctcaggacc cggggataca acctatcttc gcatcgtatg gcgggcaccg
      961 gcaggcagag atccggatat ctttccgttg gctgtgttgg acagtattct gaccggtccc
     1021 agcagtctga atatgttcgg aagcggatcg atttcaaata aaaccagccg cttatatcaa
     1081 cccttaatcg atcgacagat tgccgtcgca ttcagcggag attacaatgc cacgatcgat
     1141 ccttaccttt atatgatgac cgttattatg catccggatc atgatcctca ggaagctgtt
     1201 cggataattg acgaagaaat aagtcggatt caatccggag atatcagcga gcaagaattg
     1261 aaaaaagcgg taaaacaagc ccgtgcgtta ttcgcttacg gatcggaaaa catcaccaat
     1321 caagcctatt ggatgggtta tacggaaatg ttctcttctt attcctggtt tacggaattc
     1381 gtatcgtctt tgaataaggt gaccgtttcg gatgtcgcca aatcggcttc aacctatctc
     1441 aactcggctc atcgggtgat cgggatttat cgcccttacc cggaagaaag tgaggaatca
     1501 tgaaaaatat ccaatcaatt ccggggagtg acgacattct gcgaatagaa cttccgaatc
     1561 agatcgtact ccttgccaga gaaaacagcc tcagcccatc cgttgtgatc agcggatacc
     1621 ttcccggcgg atccgttctg gatccggtcg atcgaagcgg tctggcaatt tttctgtctc
     1681 tcgcattgat gcgcggaacc gaaacaaata gttttgaaca gattttccgc aagttggaag
     1741 ataacggtgc atctttgggt ttcaacgccg gcttccattc tttcagtttc gcgggacaat
     1801 ccttggcgga agatttgccg gtcatgcttg acttactcaa tgatatttta cgccacccaa
     1861 tatttccgga aaatcacctg acccaaatcc gtgaccaaat gctgaccgct ttggcaatcc
     1921 gttcacagga taccgctgaa atggcaaatg tcgcctttga ccaagcttta ttcggttccc
     1981 atccgtacgg gcgagataaa ctggggacac cggaaagtgt gcaatccgtt tcacgatctg
     2041 atttggtacg tcaccatgct cagtatttca agccgcaagg aatggttatc agcatcgtag
     2101 gcggaatcga acccgagaag gcggcagatt tggttcggaa atatttttcg gattgggaag
     2161 cgggacctca ggccattcca aatttgccgg attttccgaa agtcacaaaa cccgaaaaag
     2221 gaagcgccac acacattgag attccggaaa agagtcagtt ggatctggtg atcggcagca
     2281 tcgcaccgga gcgaaaatct ccgcagtatc agccggctcg cttgggaaat tgcattttgg
     2341 gagaattcgg aatgatgggg cgtatcggaa gagttgtccg tgatgaaaat gggttggcat
     2401 attatgccgg ttccgatgta aattgtttgc cgttaggcgg gtcttgggaa atttccgccg
     2461 gaatcaatcc ggaaaatctc gatcgaaccg tcgatctgat tcgttccgaa attcaaaaat
     2521 ttgtcagcac accggtcgaa ccgaatgaat tatccgatgt tcaatcatac tacttaggaa
     2581 gaatgcccct gcaattggag agcaacgccg gaatcgcaaa cgaactgctg tcgatcgaac
     2641 gatatcagtt gggactcgat tatttacgcc ggtacggaga catcatccgg gcggtaacac
     2701 gggaacagat tctcgaaacc gccgcgactt atctggatcc ggatcgactt actttggcaa
     2761 cggccgggac tttacgggga aaagcatgac aacgattctg cgatccggaa tcgatctgat
     2821 cgaaatcgaa cggattgaga aattaaaacc ggagataaaa gctcgttttt tacaaagagt
     2881 ctatacctcg gcggagctgg atttatgcgg ggatcgcgat gaacgattgg cggggcgatt
     2941 tgcctgtaaa gaagccgttt ccaaagcgct cggtaccggc atcggaaccg ttcattggca
     3001 ggagattgaa atcttgacag atgatcaaca aatgccgatt ttatacttac acggcaacgc
     3061 ggccgctttg gcaaaatctc tgggattgtc aatttggtct gtaagtatca gtcatgtcaa
     3121 aaactatgcc gtggcaatag cgatcgcttc cgattgaatc gacaaaatcc gcaagatttt
     3181 tatttcattc atcatataat gaatggatga cgataaaaat tttagcgctt tcggatgtgg
     3241 aaaccggctt gatatacagc gaacgttccg atgaatactt ccccgctgtg gatttaatcg
     3301 tcagttgcgg ggatttaccg atgagctatc tggactacgc agccacaaca ttaaacgccc
     3361 cgctgtattt tgtattaggc aatcataaca gcttgattcc ggtcagtaaa acctccaatt
     3421 acccttataa ttccagcgga tataaccttc attgttccaa ttacttttat gaaaagcgct
     3481 tattgattgc cggtgtcgag ggatcggtga aatacaacaa cggtccgcaa caatattctc
     3541 agagcgaaat gtggacaaat gtattttcat tggtaccggc tcttttaaga aacaaactgc
     3601 ttcatgggag atatcttgat ctattcgtat ctcatgcccc gccttggcag attcacgatc
     3661 agcaagactt gccgcataac ggaattcgtg catttcgttg gttggataaa gtatttaaac
     3721 cgaaaattca tttgcatggg cacatccatg tttatcgatc cgacacggtc cgggaaactg
     3781 tttattacaa caccaaagtc atcaattgtt acggttatcg tgaaattttc tttgatgaac
     3841 cgacggataa tctgccgaag gaatcatagc aaaaattaaa gttgtaattt ttttacaagg
     3901 atgagttaaa cttttcgcat gcgaatcaat aaattgacag ataaattgtt agtgctgttt
     3961 ggcatcggat gtattttcgt cttccttttt gcttcttgca gcatactcac aggtttacag
     4021 gaagtggatc ctcaaagtgc gacattaaca gctttggtaa cacccacttc cgtccagccg
     4081 acacttgatc tgaaagggaa taaggtactg gaagatcgtt cctgtttaat attcgacagc
     4141 cccagcatat ccactttcgc ggatgccgag gacggaggaa tttttgcctg ggcgggaaat
     4201 aagaatttgc ttgctttcgt tgttccggaa aaccgctatt gggcatggtt ttccggagat
     4261 gcggaaatcg tggattttga aaaagattat aagaacccga ccattatgaa taccaccggg
     4321 ctgaaagttt tcggagattt cgcatttaac ccaagcgcca ccaaaattgc ctttgttgtt
     4381 ttgcgtacct cggagaagat ttataccgta gtggtagcga cgctttcgtc aaacctccag
     4441 acgaccattg acttatttac cgatacatcg gctcgtacgg ataattacag cagctcgaaa
     4501 tcggtcatcc aatgggaaaa tgatgatgaa gttaccgtta cgaccagttg cggcatcgat
     4561 tgcgatcaaa tttatcggat caatacggtg accggcacaa cccggttgat cgatgaagtc
     4621 cggaaaaaag ggcacaccgg cagagatttt ccgcaaaacg ttccggaata tgacgaacgt
     4681 gaatatccgg caatgaacca acctaactgg tcaccggatt taagtatggt gttttatacg
     4741 gatgatcaaa ataagacttg gattttaaat gataaaacca aagaacaatt cgaacttccg
     4801 attaccgccg acaaagtcct ccagactgcc tggtcccatg acgctcaatt cattgccatc
     4861 cgttttgccg aaaacttatc cgtatataaa gtgaattgta aata
//
LOCUS       DAOMIV010000093         4880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_17653_length_4880_cov_3.436684, whole genome shotgun sequence.
ACCESSION   DAOMIV010000093 DAOMIV010000000
VERSION     DAOMIV010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17653_length_4880_cov_3.436684"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKV59_03345"
     CDS             <1..430
                     /locus_tag="PKV59_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQP45819.1"
                     /translation="VGAILRTADGAGIRKIFLCGITPTPENPKVLKTSLGAENAIRWK
                     HSWNILEVVEQLKSEGFLILCLEGGKRSINLFQNIGKILSGQPVALFIGNEINGIDPV
                     VLEKADFSVFLPMQGEKESLNVATAFGIAAYLLRYGKTIK"
     gene            427..3021
                     /gene="mutS"
                     /locus_tag="PKV59_03350"
     CDS             427..3021
                     /gene="mutS"
                     /locus_tag="PKV59_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQP45820.1"
                     /translation="MSEEFTPIRQQYLEIKKQYPDTIVFFRLGDFYETFDEDAETTSR
                     ELDLVLTSRNVAKGVRVPMAGIPYHAVENYLSRLIDKGYHVAICEQIGDQPTKGLFER
                     EVVRVVTPGTLIEPGLLKSDSNNYLMAACVENDKIGIAYADITTGEFAVTQIQSADIT
                     ADLQSEITRLNPAEILVAQSFDLPLSENLHITRVPDYNFEPLRCTELLRDHFQVATLD
                     GYGLKENSIPVRAAGCLLQYLQDNQPSSIKLLSSISFYSLDDFMVLDSSTRRNLELTE
                     SLRNNELKGSLLGVIDCTVTPMGKRVIRQWVSKPLTNQNGIQNRLDAVESFFNDGMIR
                     AEVRESLKGMNDMERLINRISGGMAIPRDLTALRNSIRKLPEIIEIIEPILPALTPII
                     QKIYPLEAVKQILESAVADDPPATLMNTGVIRAGYSEELDRIHQESGHAREWMANLES
                     SERERTGIKTLKVSYNKVFGYYIEISRGSADQAPPEYIRKQTLVNAERFITPEMKEYE
                     ALVLNAEERIREIENRLFYQICAEISQYATEILTTARGIATLDVLAALAESAVRNKYV
                     KPEINNSLEIEINEGRHPVVEQTLPQGTRFIPNDIKLGGNSFIHVITGPNMSGKSTFL
                     RQTAIIVLMAQMGSFVPASSAKIGLVDRIFTRIGAQDEIHAGLSTFMVEMVEAANILN
                     NGTDRSLLILDEIGRGTSTYDGLSIAWAIIEYIHNAPGLKSRTLFATHYHELTSLANS
                     LPGVKNYNVAVTENAGEVVFLHKIIPGAADRSYGIHVAQIAGLPKPVISRANEILKQL
                     ESDSVKQMEIRETPSYQLTLFPETNPIIDTLDRLDVNSMTPIEAINILYEWKQRFGKS
                     DSNKKKTG"
     gene            complement(3144..4100)
                     /locus_tag="PKV59_03355"
     CDS             complement(3144..4100)
                     /locus_tag="PKV59_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQP45821.1"
                     /translation="MSIVCSGSVAYDYLMRFPGYFRDQILPDHLDSISLSFLVDDMVR
                     LQGGVAPNVCYSLALLGEKPILFAAVGEDFDQYRKWLEERGVDCRFARVIPGKFTASF
                     FSNTDLANAQIASFYTGAMKNSADLKVSELKDEKVDLFLISPSDPDAMIGYARQCKEL
                     GIPFGFDPSQQIVRMNREMIREGLDGAKFLFCNEYEFELLKKHSEWSEEEVLDRVDCA
                     VITFGEKGSRIYADGSVTTVPVYPPERIVDPTGVGDAYRSGFLTGYYKGLDWELCGKM
                     GALASTYVLEQKGTQSHRYDRETFIERFRKLWDDGGRLDCLL"
     gene            complement(4123..>4880)
                     /locus_tag="PKV59_03360"
     CDS             complement(4123..>4880)
                     /locus_tag="PKV59_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP45822.1"
                     /translation="EPYESPELKNICDEFKLDSTFGALPFDYGHVCINYDTTYFTEHN
                     LAIPTSLDDLISEPYKGLLVVENPTSSGPGQAFFLTTVAEYGEDGFVDYWNKLLANDV
                     QIDNDWSSAYYTSFTAGGGNGPRPMVVSYDTSPAAVPFYSETPMETAPTAAVLADGTC
                     YRTIEFLGILKGTKNREIAEKFVDAMLGKRWQEDLPGQMFVYPVNREAVLPEVFAKFA
                     IQPENPVSLDSALIEEKYEDWIQTWNDEVLVNY"
BASE COUNT         1376 a         1172 c         1070 g         1262 t
ORIGIN      
        1 tgtcggagcc attctcagaa ccgctgacgg agcgggtatt cggaaaattt ttctttgcgg
       61 tatcacccct actcccgaaa atccgaaggt cttaaagaca tccttgggag cagaaaatgc
      121 gatccgatgg aaacactcat ggaatattct ggaagttgtt gaacaattaa aatcggaagg
      181 tttcctgatt ttatgcctgg aaggcgggaa gcgttcgatt aatttattcc aaaatattgg
      241 taaaatttta tccggacaac ccgttgctct gtttatcgga aatgaaatta acggaatcga
      301 tcccgttgtc ttggaaaaag ctgatttcag tgtatttctt cccatgcagg gagaaaaaga
      361 atctctgaat gtcgccactg ctttcggcat tgcagcttac cttttacgtt acggaaaaac
      421 aattaaatga gcgaagaatt tactcccatt cgacaacaat accttgaaat taaaaaacaa
      481 taccccgata caatcgtttt ctttcgattg ggagattttt atgaaacctt cgatgaagac
      541 gccgaaacaa cttctcgtga actggatttg gtgctgactt ctcggaatgt tgcaaagggt
      601 gtacgggtgc cgatggccgg catcccttat catgccgtcg agaactatct gtctcgattg
      661 atcgataagg gctatcatgt cgcaatttgt gagcaaatcg gggatcagcc cacgaaaggt
      721 ctctttgaac gggaagtggt gcgtgtggtg actccgggca ctttaattga acccggatta
      781 ttaaaaagcg attccaataa ttatctgatg gcagcctgtg tcgaaaatga caagatcggg
      841 attgcatatg cggatatcac caccggtgaa tttgccgtca ctcagattca aagtgcggat
      901 attaccgctg atttacaatc ggaaataacc cgtctcaatc cggcagaaat attggtcgca
      961 cagtcttttg acctgccgct ttccgaaaat ctgcacatta ctcgcgtgcc ggattataac
     1021 ttcgaaccgt tacgctgtac cgaactgctc cgggatcatt ttcaagtcgc cacgctggac
     1081 ggatacggtc tgaaagaaaa tagtataccg gtcagagccg ccggctgctt attgcaatat
     1141 ttacaagaca atcaaccttc aagcataaaa ctgctttctt caatttcttt ctattctttg
     1201 gatgatttca tggtgcttga ttcatctaca cgcaggaact tggagttaac cgaatccctg
     1261 cgtaataacg agctcaaagg ttcgctgttg ggtgtaatcg attgcaccgt cactccgatg
     1321 gggaaaaggg ttattcggca atgggtcagt aaaccgctca ccaatcaaaa tgggattcaa
     1381 aatcgtttgg atgcggtgga atcattcttt aatgacggga tgatcagagc ggaagtgcgg
     1441 gaaagcctga agggaatgaa tgatatggaa cgtttgatca accgaatttc gggtgggatg
     1501 gcaatcccgc gtgatcttac cgctctgcgt aattcaattc gcaaacttcc ggaaatcatc
     1561 gagattatcg aaccgatttt gccggctctg acgccgatca ttcagaaaat ctacccgttg
     1621 gaagccgtta agcaaatatt ggaatcggct gtcgcggatg atccgcctgc cactctgatg
     1681 aacaccggtg tgattcgcgc aggttattcg gaagaattgg atcgaattca tcaggaatcc
     1741 ggccatgctc gggaatggat ggctaatctg gaaagcagcg aacgggaacg gaccggtatc
     1801 aaaacgctga aagtcagcta taacaaagtt ttcggatatt atatcgaaat ctctcgcggg
     1861 agcgcggatc aggcaccacc cgaatatatt cggaagcaaa cactcgtgaa tgccgaacgt
     1921 ttcattacac ccgaaatgaa agaatatgaa gcgctcgtgt tgaacgcgga agagcgcatt
     1981 cgcgaaatcg aaaatcggtt gttctatcaa atctgtgccg aaatcagtca atatgcaacg
     2041 gaaattctga ccaccgcaag agggatcgcc acattggacg ttttggctgc tttagcggaa
     2101 tccgccgttc gcaataagta cgttaaaccg gaaattaata acagcctgga gatcgaaatt
     2161 aacgaaggaa gacatccggt cgtcgaacag acacttcccc aaggaacccg ttttatccca
     2221 aatgacataa aattgggagg aaattccttt attcacgtca tcaccggtcc gaacatgagc
     2281 gggaaaagca cttttttacg tcaaaccgca atcatcgttc tgatggctca gatgggttct
     2341 tttgtccccg catcttccgc aaaaatcggc ttggtcgatc ggatattcac ccgaatcggt
     2401 gcccaggatg aaattcacgc cggattatcg acatttatgg ttgaaatggt cgaagcagcc
     2461 aacatattaa ataacgggac cgatcgcagc ttgctgatcc ttgatgaaat cggacggggg
     2521 accagcacgt atgacggcct ttccatcgct tgggcgatta tcgagtacat tcataacgcc
     2581 cccggtctga aatccagaac attattcgct acccattatc atgaattgac aagtcttgcg
     2641 aattccttgc cgggcgtaaa aaattacaat gtggccgtca ctgaaaatgc gggagaagtt
     2701 gtatttctac ataagatcat tcccggtgcc gcggatcgtt cttacggtat tcatgtcgct
     2761 caaatcgccg gcttgccaaa accggtcatt tccagggcaa acgaaatttt gaaacaattg
     2821 gaatcggatt cggtcaagca gatggaaatt cgagagacgc cgtcttatca gttgacttta
     2881 tttccggaaa ctaatccgat tatcgatacc ttggatcgat tggatgtcaa cagcatgacg
     2941 ccgattgaag caattaacat cctttacgaa tggaaacaac gtttcggaaa atcggattca
     3001 aataaaaaaa agaccgggta acctcggtaa ccggtcactc ggtctcgcat cattctccaa
     3061 agatcatttc gctcaatgct ctattctctt tttttttctg tctttgcact aagtaaaaac
     3121 cattttccaa cgaaaagccc ggtttataag aggcaatcca atcgtccgcc gtcgtcccat
     3181 aattttcgaa aacgttcgat aaaagtttcc cgatcataac ggtgagattg tgttcccttt
     3241 tgttccaaaa cataagtgga cgccagagct cccatcttcc cgcataattc ccaatccagt
     3301 cccttataat aaccggtcaa gaaaccggat cgataagcat cgcccactcc ggtcgggtcg
     3361 acaattcgtt cgggcggata aacgggcacc gtcgtaaccg atccgtcggc ataaatacgc
     3421 gatccttttt ctccgaacgt aatcacggca caatccactc tatcaagaac ttcttcttcg
     3481 ctccattcgc tatgtttttt cagaagctca aattcatatt cattgcaaaa caagaatttt
     3541 gcgccatcca acccctcgcg aatcatctct ctgttcatgc gaacaatttg ctgagacggg
     3601 tcgaatccga acggaatccc aagttctttg cattgccggg catacccgat catggcatcc
     3661 ggatcggatg gtgaaataag aaacaaatca accttctcat ctttcagctc ggatactttc
     3721 agatcggctg aattcttcat tgcccccgta taaaatgaag caatttgagc gttagccaaa
     3781 tccgtattgg aaaagaaaga agcggtaaac ttacccggta tgacccgtgc gaagcggcaa
     3841 tcaacgcccc gctcttccag ccattttcga tactgatcaa agtcttcacc gaccgctgca
     3901 aacaagatcg gcttttcccc caacaaagcc aggctgtagc agacattcgg agccacaccg
     3961 ccttgaagcc gcaccatgtc atcgaccaaa aaagacaggc tgatcgaatc caagtgatcc
     4021 gggagaattt ggtctcgaaa atatcccggg aaacgcatca gataatcata tgctaccgaa
     4081 ccgctgcaga ctattgacat ttataatttc tcttctttcc gtttagtaat tcaccaatac
     4141 ttcgtcgttc caggtttgaa tccaatcttc gtatttttct tcgatcaatg ccgaatccaa
     4201 tgaaaccgga ttttcgggtt ggatcgcgaa ttttgcaaag acctcaggca aaaccgcttc
     4261 tcggttaacg gggtacacaa acatctgtcc gggtaaatcc tcttgccaac gtttccccag
     4321 catggcatcc acaaatttct cggcgatttc acgattcttg gttcccttta atattcccaa
     4381 aaattcgatc gtccgatagc acgtaccgtc tgccagaacg gcagccgtcg gagccgtctc
     4441 catcggagtc tccgaataaa aaggaacggc agccgggctg gtatcatatg aaacgaccat
     4501 cggtcgcgga ccgtttccgc ctccggccgt aaaactggtg taataagccg aagaccaatc
     4561 attatcgatt tgcacatcgt ttgccaacag tttattccaa taatcgacaa atccgtcttc
     4621 gccgtactcg gcgacggtgg tcaggaaaaa agcctgtccc ggacccgaag aagtcggatt
     4681 ttcaacgacc aaaagccctt tgtacggttc cgaaatcaaa tcgtcgagag aggtaggtat
     4741 cgctaaatta tgctctgtga aataagtggt atcgtaatta atacagacat gcccataatc
     4801 gaacggtaac gcaccaaagg tggaatccag tttaaattca tcacaaatat tcttcaattc
     4861 cggcgattca tatggttcaa
//
LOCUS       DAOMIV010000094         4863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_17742_length_4863_cov_5.040765, whole genome shotgun sequence.
ACCESSION   DAOMIV010000094 DAOMIV010000000
VERSION     DAOMIV010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17742_length_4863_cov_5.040765"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..2156)
                     /gene="fusA"
                     /locus_tag="PKV59_03365"
     CDS             complement(84..2156)
                     /gene="fusA"
                     /locus_tag="PKV59_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQP45823.1"
                     /translation="MAREYPLEKYRNIGVIAHIDAGKTTTTERILFYTGKTYRVGSVD
                     EGTTVTDWMDQERERGITIVSAAVTAEWKGYRINLIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDATQGVEPQSETVWRQADKYNVPRICFINKMDRTGASYHDSVESIRNRLGANPV
                     EMQIPIGSEGNFSGVIDILKQKAYSWEDELGINMIEFDIPADKVEEAQAAREQLVEYI
                     AGLDDDLTNRFLEGEELTNEMLIAGLRRAVIAGKCNPCYCGSSLKNKGVQLLLDAVVD
                     LLPSPLDIPPTKGTSLDGSKEIWLKPDDSAPVGSLVFKIVTDPYVGRLAYFRVYSGTV
                     KQGQMLLNPVKGKKERIGRLIRMYADRRDDVDEVYAGDIAAVLGLKDTFTGDTLCDPH
                     HPVVLESITFPEPVISVAIEPKTLADQEKIGLALQKLSEEDPTFRVRTDENTGQTIIS
                     GMGELHLAIIVDRLMREFRVRANVGKERVAYKETIRKAVEKHSYKYAKQTGGHGQFAH
                     VVLTVEPIDANLGVQFENKIVGGVIPKEYIPAVEKGVREAAESGVLGGYPVTGIRVSL
                     VDGSFHEVDSSEMAFKLAGSICLKECLELADPVLLEPIMKVEITIPEEYLGDVLGQIS
                     SRRGEIRGTDIHGGNSQIVHSMVPLGEMFGYATTLRSATQGRGVFAMEYDHYQAVSDA
                     TAQKILNI"
     gene            complement(2180..2647)
                     /gene="rpsG"
                     /locus_tag="PKV59_03370"
     CDS             complement(2180..2647)
                     /gene="rpsG"
                     /locus_tag="PKV59_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQP45824.1"
                     /translation="MRRTKPEKREVTPDVRYNSAVVTNLINRIMHNGKRSNAVRMVYS
                     AMDIVSQKTGKDGLEVLETALRNVGPVMEVRPRRVGGATYQVPMEVSANRRQTLAMRW
                     IIDASRSRTGKAFDECLAGELLDAYNNTGSAIRKREETHKMAEANRAFSHYRV"
     gene            complement(2663..3082)
                     /gene="rpsL"
                     /locus_tag="PKV59_03375"
     CDS             complement(2663..3082)
                     /gene="rpsL"
                     /locus_tag="PKV59_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQP45825.1"
                     /translation="MPTINQLIRKGRKTRSVKSKAPALQYTYNSSNQRRTKVAEGAPQ
                     KRGVCTNVRATTPKKPNSALRKIARVRLSNGFEVTAYIPGEGHSLQEHSVVLVRGGRV
                     RDLPGVRYHIVRGTLDTTGVAKRGQGRSKYGVKRAKK"
     gene            3567..>4863
                     /locus_tag="PKV59_03380"
     CDS             3567..>4863
                     /locus_tag="PKV59_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HQP45826.1"
                     /translation="MKKSFLFVVSILLISTLILTGCGSKATETPKPATKVVEEATTAP
                     SATTAPSATTEPSATTSSQADAAVEAVENIVSTAANSAETAIENAEKTVGEAAATAVD
                     TAKTAVESVEKSGSEAGASDVMKAKGVADKSRCDAEGGCAVIKPGDTIKIGMGGPLTG
                     EYAMYGVDISDSEKISISDLKPLEGFEWELLAEDTVGSPETAVSIANKWVTDDTFVAV
                     AGNVLTGESAAIIPIMEKYYIPMLSPSATGANLTENNKVFNRIVFQDAEQGKFAAEFI
                     VNTLGYKKVAVLHDGMDYGKGIADTVRDTLVKLGVEPVAYEAITPGEADYSAVLTSIA
                     SKKPEIIYYGGYTAELSVIANQLGQVGLAGTPVFSDDGAFGAEFIEKAGKNAEGCYGT
                     SSVPADSEAKKAFDKKYKETLGREPGSKSTFPWFAYDVIQ"
BASE COUNT         1317 a         1233 c         1012 g         1301 t
ORIGIN      
        1 ccatggtccc cacattcaca tgtggtttct ttcgctcata atgctgtttt gccataaaac
       61 aaattctcct cagaaaactt atattatata tttaatattt tttgtgcagt tgcatccgaa
      121 actgcttgat agtgatcata ttccatcgca aacacacccc ggccctgtgt ggcagagcgt
      181 agtgtcgtcg cgtatccgaa catttctccc aacggaacca ttgaatgaac aatttgcgaa
      241 tttccgccgt ggatatccgt tccgcgaatt tctccgcgcc ggctgctgat ttgtcccaat
      301 acgtctccca aatattcttc cggaatcgta atttcgactt tcataatcgg ttccaacaat
      361 acgggatccg ccaattccaa acattctttc agacaaatac tgccggccag tttgaatgcc
      421 atttcactcg aatcgacttc atgaaacgag ccgtccacca acgatactcg tattccggta
      481 accggataac cgcccaacac accggattcc gccgcttccc ggacgccttt ttccaccgcc
      541 ggaatatatt ccttcgggat tactcctccg acaattttat tctcgaattg gacgcccaag
      601 ttcgcatcaa tcggctcgac cgtcagaacg acatgagcaa attgcccgtg accgccggtc
      661 tgtttcgcat atttatagct gtgcttttcc accgcttttc gaatcgtctc tttataggca
      721 acccgttctt tcccgacatt cgcccgaact ctgaattcac gcatcaaccg atcgacgata
      781 atcgccaaat gcagttcgcc cataccggaa ataatggtct gtcccgtatt ttcatccgtt
      841 cggacacgaa acgtcggatc ttcttcagat aatttttgca gagccaatcc gattttttcc
      901 tgatcggcca atgttttcgg ttcgattgcc accgaaataa ccggttccgg aaaagtgatg
      961 gattccaata caaccggatg atgaggatca caaagcgtat cgccggtaaa tgtgtccttc
     1021 agccctaaga cggcggcaat atcccccgca tatacttcat ctacgtcatc tctgcgatca
     1081 gcgtacattc ggataagcct gccgatccgt tcttttttcc ctttgaccgg atttaaaagc
     1141 atttgacctt gctttacagt cccagaataa acccgaaaat atgccaaccg tccgacatat
     1201 ggatcggtga caattttgaa taccaaagaa cccaccggtg cggaatcatc gggttttaac
     1261 caaatttcct ttgaaccgtc caggctggtc ccttttgtcg gcgggatatc taaaggtgac
     1321 ggaaggagat cgacaacggc atccaacaaa agttgaacac ctttgttttt taaggaggat
     1381 ccgcaataac aggggttgca tttccccgca atgaccgccc ggcgcaatcc ggcaatcagc
     1441 atctcatttg tcaattcctc gccttcaagg aaacggtttg ttaaatcgtc atccaatccg
     1501 gcaatgtatt ccaccaattg ttcacgggcg gcttgtgcct cttcgacttt atccgcaggg
     1561 atatcgaatt caatcatatt gatgcctaat tcgtcttccc atgaataagc cttttgcttc
     1621 aaaatgtcaa taacgccgga aaaatttcct tccgacccga tcgggatttg catttcaacc
     1681 ggattcgccc ccaaacgatt tcgaatggat tcgaccgaat catgataaga agcacctgtc
     1741 cgatccattt tattgataaa gcagatacgc ggcacattat atttatcggc ctgtcgccag
     1801 accgtctcac tttgcggctc aaccccttga gtcgcatcaa aaacaacaac tccgccatcg
     1861 aggacacgta aagaacgttg cacttccgca gtaaagtcga tatgtccggg agtatcgatc
     1921 aaattaattc gatatccttt ccattccgct gtaaccgcag ccgaaacaat cgtaattcct
     1981 cgctcccgtt cctgatccat ccagtccgtt acagttgtac cctcatcgac tgaaccaacc
     2041 cgataagtct ttccggtata aaacagaatt cgttcggttg ttgtcgtctt gcccgcatcg
     2101 atatgagcaa taacgccaat atttcgatac ttctctaacg gatattcacg agccatatag
     2161 gattactcaa ttctttttct caaactctgt aatgagaaaa cgcacggtta gcttccgcca
     2221 ttttatgggt ttcttcgcgt ttccgaattg cgctgccggt attgttataa gcatcgagta
     2281 attcacccgc aaggcattca tcaaatgctt taccggtgcg agaacgtgat gcatcaataa
     2341 tccagcgcat ggccagagtc tgtcttcgat ttgccgatac ttccatcgga acttgatagg
     2401 ttgcaccgcc gacacgccgc gggcgaactt ccatcacagg accgacattt cgtaaagcag
     2461 tttccagaac ttcaagtccg tctttccccg tcttttgact gacgatatcc atggcactgt
     2521 ataccattct gactgcgtta cttcgttttc cgttatgcat aatacgattg attaaattcg
     2581 tcacaacagc actgttataa cgaacatccg gggtaacttc ccgcttttct ggtttagtac
     2641 gtcgcattca acttctccta atttatttct tagcccgttt tacgccgtat tttgaacggc
     2701 cttgtccacg tttcgctaca ccggtcgtgt ccaatgtacc gcgaacaata tgataacgaa
     2761 caccgggtaa atctctgaca cgtccacccc gaaccagaac gacactgtgt tcctgaagag
     2821 aatggccttc accgggaata taggcagtca cttcgaaacc gttggacaaa cgaacacggg
     2881 caatcttccg caaagccgaa ttcggcttct tcggcgtagt cgcacgaaca ttggtgcata
     2941 caccacgctt ttgcggtgcg ccctcagcta ctttagtacg acgctggttg ctggaattat
     3001 aggtgtactg caacgccggc gccttacttt tcactgatcg ggtcttccga ccttttctga
     3061 tcaattgatt aatcgttggc acttcaaaaa gcctccataa ctaaaagata cttaattttt
     3121 ctttccctcg gctgtttcta tttttatgag tgcaacccga ggttatttac acacaataaa
     3181 tactaagccg gtttgacacg gattagccaa aagattatat cacattcaaa tcaagtgagg
     3241 aaatattccg ctccgccgaa agaaaataat gacagaaaaa gtggagtaat caatatcccg
     3301 acagattgat aaaaaccatt ctcagaaaat cataaaataa attgtaataa gaaattcaga
     3361 ttgacttgac agtcattttt ttcttgagta atattctgaa ttactgcggg aggcataatt
     3421 aatccgcaaa agatactaca agtgtccttt tgaattgaat gttaatcgaa taagagaaaa
     3481 ataaacctaa tttgcaaaaa catgggtgaa atatattaat tcaacaattt taaagggcaa
     3541 atctaaattt tttcaaggag agaaatatga aaaaatcgtt cttgtttgta gtcagtatat
     3601 tgcttatctc taccttaatt cttaccggat gcggaagtaa agccacagaa actcccaaac
     3661 cggcaactaa ggttgtagag gaagccacca cagcgcccag tgccacaacc gcacccagcg
     3721 ccacaaccga acccagtgca accacgagtt cccaggcgga cgccgccgtg gaagctgtgg
     3781 agaatattgt ctcgaccgca gcgaattctg ctgaaacagc tatagagaat gcggaaaaaa
     3841 ccgtgggtga agctgctgcc accgctgttg acaccgcaaa aacagccgtt gaaagtgtcg
     3901 aaaaatccgg cagtgaagct ggcgcatccg atgtgatgaa agccaaaggc gtggctgata
     3961 agtcccgctg tgacgcggaa ggtggttgcg cggtgattaa accgggagat accattaaaa
     4021 tcggtatggg aggtcctctc accggtgaat acgccatgta tggtgtggat atttccgaca
     4081 gcgagaaaat atcgatttcc gatctgaagc ctctggaagg attcgagtgg gaacttttgg
     4141 cggaagatac cgtcggctca ccggaaacag ctgtttccat cgctaacaaa tgggtgaccg
     4201 atgatacgtt tgtcgcagtt gccggtaatg tattaaccgg tgaatccgcc gcgattatcc
     4261 cgattatgga aaagtactac attccgatgc tttctccttc cgccaccggt gccaatctga
     4321 ccgaaaacaa taaagtattc aaccgaattg ttttccaaga tgccgaacaa ggcaagtttg
     4381 ccgcagaatt catcgtcaat acattaggct ataagaaggt tgcggttttg catgacggca
     4441 tggattacgg aaaaggaatt gccgataccg ttcgcgatac gctggttaaa ttaggcgttg
     4501 aacccgtcgc ttatgaagcg atcactccgg gcgaagcaga ctactctgca gttttgacct
     4561 cgattgcttc caagaaaccc gaaattatct actacggcgg ttatacggct gaattatccg
     4621 tcattgccaa ccaattaggg caagtcggtc tggcgggtac acctgtcttc agtgatgacg
     4681 gcgctttcgg agcggaattc atcgagaaag ccggaaagaa tgccgaaggt tgctacggta
     4741 catcctctgt tccggctgat tcggaagcga aaaaagcatt cgacaagaaa tataaagaga
     4801 ctctcgggcg tgaaccggga tcaaaatcga ctttcccatg gttcgcttat gatgttattc
     4861 agg
//
LOCUS       DAOMIV010000095         4860 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_17759_length_4860_cov_3.198543, whole genome shotgun sequence.
ACCESSION   DAOMIV010000095 DAOMIV010000000
VERSION     DAOMIV010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17759_length_4860_cov_3.198543"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKV59_03385"
     CDS             complement(<1..339)
                     /locus_tag="PKV59_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HQP45827.1"
                     /translation="MFENLTERLNKIFAGLTRRGKLSAEDVDAAMREVRIALLEADVH
                     YSVVKTFVANVRERAIGTEVSEALNPGQMVIKIVNEELIKTLGPAEKLNLSGEKPHVI
                     MLVGLQGSGKT"
     gene            562..2964
                     /locus_tag="PKV59_03390"
     CDS             562..2964
                     /locus_tag="PKV59_03390"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45828.1"
                     /translation="MRFGFFDDAKREYVVEQVDVPVSWTNYIGTKNMCGVFNHTAGGY
                     LFCGSPEYHRITRFRPNGVPMDFPGHYVYIRDDDTGEFWTISWQPAGLPLDKAKYQCR
                     HGLSYSVYSCEYREISASQKLFVAIDDPLEIWDVNIQNQSDCQRHLSIFSYLEFSFHH
                     IAMDNQNFQMSLYAAGSRYQDGIIEYDLFYEEDGYQFFTADFEPDGFDCVRDRFIGPY
                     RTEKNPIAVESGHCSSSFEKGGNHCGTLRKQLSIDPNEKIRLIFLLGEGPLEEGRKIR
                     KKYADPHEVDRQFNLLRTFWDEKCSKLQIQTPDDGMNSLINIWTLYQSEINVTFSRFS
                     SFIEVGGRTGLGFRDSAQDAMSIPHSDPVKCKSRIVELLRALTSEGYGLHLFDPAWFE
                     PQQAKKPFKSPTIIPVPEKSSMIHSVDQACADDALWLIGAIHNYLKETGDFDFVWEEI
                     TYADKGSGSVYDHMKRILDFSEKQVGKHGICKGLRADWNDCLNLGGGESALVSFLHYW
                     ALTQFSELADFLGEKKDAEHYRHVAEHVRQVCEEVLWDDGWYIRGITADGRKIGCHQD
                     VEGKVHLESNAWAVLSGVASRERGEQCMNAVEEYLFTPYGLMLNAPPYTKPDDGIGFV
                     TRVYPGLKENGSIFSHPNPWAWAAECTLGHGDRAMRYYDALSPYKQNDQIEIRQAEPY
                     SYCQFIVGKAHSAFGRARHPFMTGTAGWAYYAATQYMLGIKPGFTTLSIDPCIPESWD
                     GFRVKRIWRGAEYDITVENPDNVQKGVKALFCDGLPVAEIPLKPCGTSTNVKVVMGKQ
                     TI"
     gene            2961..4394
                     /locus_tag="PKV59_03395"
     CDS             2961..4394
                     /locus_tag="PKV59_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HQP45829.1"
                     /translation="MIHFGTGGWRAIIGDEFTKSNIQLLSYSIGDLMEKTGTANLGLC
                     IGYDRRFLSKEAAHWAAEVFAARQIPCYVIDRSSPTPLIMFTVKQMNLPYGMTITASH
                     NPAIYNGVKFFTAGGCDANEDVTNEIEFLTQKNAEVKIPFLDYDEGIRSGIIKEINPL
                     NSYIDSILSLVDTEAIKKARLRIALDPMYGVSQTSLQTILLTARCEVEVIHDRHDTLF
                     GGRLPAPNIHTLGTLVNFVLDHHCDLGIATDGDADRLGVIDDKGQFVHPNKLLVLLYY
                     YLLKYRGWRGPAVRNNSTTHLLDKVAAGFGQACYEVPVGFKYVSAKMEETGAIIGGES
                     SGGLTVKGHIRGKDGIYAATILTEMVAVTGKHLSELYKEITDQFGEVFNSDADFKLTP
                     QNKERLQNILYVENKLPDFPYEIDHVSYRDGCKVYFKNSGWVIVRFSGTEPLIRLCAE
                     MSDQKSADQLISVFSDFLGLESELQSQ"
     gene            complement(4427..>4860)
                     /locus_tag="PKV59_03400"
     CDS             complement(4427..>4860)
                     /locus_tag="PKV59_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQP45830.1"
                     /translation="SRMSEINEALQFMTDTLARQSPAASDDKIDDLSTASANSFIVRQ
                     ALAYIEENFSRKLTLQDVADYCYVSQWHLSKLLNKVTGQSFYDLLNKARIDAAKRLLE
                     DPKLRISDISEMVGYTDNGHFSRLFKKMVGISANDYRNQVK"
BASE COUNT         1352 a          982 c         1118 g         1408 t
ORIGIN      
        1 tgtttttccg gatccctgta aacccaccag catgattacg tgcggctttt ctccgctgag
       61 gtttaacttt tcggcgggac cgagtgtttt tattaattct tcattgacaa tcttaatgac
      121 catttgtccg ggatttaaag cttcggatac ttccgttccg atcgctcgtt ccctgacgtt
      181 tgcgacaaat gttttcacga cagaataatg aacatccgct tcgagcaatg caatccgaac
      241 ttcacgcata gcggcatcga catcttcggc gctcaatttt ccgcgccggg tcaatcccgc
      301 aaatatttta ttcaatcgtt cggtcagatt ttcaaacatt ttttctgttt attcaaccat
      361 atctaaaaaa ataatcggtc ggttcccgac agcgagtcaa ttttagcgta aaacggctcc
      421 tctgtaaagg acacccacgc aattgtacta taacaaccgg attcaataat tgacagtatt
      481 atccatttct ttttctttcc gaaaatttta taatcggaat atattttcat aattcaaatt
      541 ttccgattcc gaggatttat tatgcgcttt gggttttttg atgatgccaa aagggaatat
      601 gtggtagaac aggttgatgt tcccgtttcg tggaccaatt atatcgggac aaaaaatatg
      661 tgtggtgtgt tcaatcatac agcgggggga tatctgttct gcggttcacc ggagtatcac
      721 agaattaccc gattccgacc aaacggtgtc ccaatggatt tccctggtca ctatgtctat
      781 attcgagatg atgatacggg agaattttgg acaatttcat ggcagcctgc cggattaccg
      841 cttgataaag caaaatatca atgccgccat gggctttctt actccgttta ttcctgcgag
      901 tatcgggaaa tctcagcttc gcaaaaatta ttcgtcgcaa tcgatgatcc tttggaaatt
      961 tgggatgtaa atatacaaaa tcaatccgat tgtcagcgtc atctcagtat cttttcctat
     1021 cttgaattct catttcatca tatcgcgatg gataaccaaa attttcaaat gagcctgtat
     1081 gccgccggat caagatatca agacgggatc attgaatatg atctctttta cgaagaagat
     1141 ggttatcagt tttttaccgc cgattttgaa ccggatggtt tcgattgtgt gcgggaccgc
     1201 tttattggac cgtaccgcac cgaaaaaaat ccgattgcgg tggaatcggg tcactgttcc
     1261 tcaagctttg aaaaaggagg caatcactgt ggaactctgc ggaaacagct ttcaatcgat
     1321 ccgaacgaaa aaatccggct aatctttttg ttgggggaag gtcctttgga agagggcagg
     1381 aaaattcgaa agaaatacgc ggatcctcat gaagtagatc gccaatttaa tctcttaaga
     1441 actttttggg atgaaaaatg ttcgaaactg caaattcaaa ctcccgacga tggaatgaat
     1501 tcgttaatta atatttggac cctttatcaa agcgaaatca atgttacatt ttctcgattt
     1561 tcatctttca ttgaggtggg cggacgtacg ggtttgggtt ttcgggacag cgcacaagac
     1621 gcaatgagta ttccccattc cgatccggtc aaatgtaaga gtcggatcgt cgaactttta
     1681 agggctttga catcggaggg gtatgggttg cacttatttg atcccgcttg gtttgaaccg
     1741 caacaagcga agaaaccgtt taaatcgccg actattattc ccgttccgga aaaatcaagc
     1801 atgattcatt cggtggatca agcttgcgcg gatgacgcat tatggttgat cggagcgatt
     1861 cataattatc tgaaagaaac cggagatttt gattttgtat gggaagaaat aacttatgcg
     1921 gacaaaggat ccggatcagt ttatgaccat atgaagcgaa ttcttgattt ttccgaaaag
     1981 caggtgggga aacatgggat ttgcaaagga ttacgtgccg attggaacga ttgcctgaat
     2041 ctcggcggcg gagagagtgc gttggtcagt tttcttcatt attgggcact gactcaattt
     2101 tcggaattag ccgacttttt aggcgaaaaa aaggatgccg aacattatcg gcatgttgcc
     2161 gaacatgtca ggcaagtgtg cgaagaagtg ctttgggatg acggttggta tattcgagga
     2221 ataaccgcag acggaaggaa aatcggctgt catcaagatg tcgaaggcaa agttcatttg
     2281 gaaagtaatg cctgggcggt tttgtcgggt gtcgcctcca gggaaagggg agaacaatgc
     2341 atgaatgcgg tagaggaata tttattcact ccctacgggt taatgctgaa tgccccccct
     2401 tacacaaaac ccgatgacgg tatcggattc gttaccagag tttatccggg actgaaagaa
     2461 aacggttcca tattttcgca tcccaaccca tgggcgtggg ctgcggaatg taccttgggt
     2521 cacggggaca gagctatgcg ctattacgat gccctttctc cgtataagca aaatgatcaa
     2581 atcgaaatca ggcaggcaga gccctattca tattgccaat ttattgtcgg gaaagcgcat
     2641 tcggcattcg gaagagccag gcatccgttc atgacgggga cagccggatg ggcttattat
     2701 gcggcgacac aatatatgtt ggggatcaaa cccggattca ccacactttc gattgatccc
     2761 tgtattccgg aatcatggga tggattccgg gtcaaaagga tatggcgggg agcggaatat
     2821 gatattacgg tggaaaatcc cgacaatgtt caaaaagggg tcaaagcgct gttttgcgat
     2881 ggcctcccgg tagccgagat accgcttaaa ccatgcggta caagcacaaa tgttaaagta
     2941 gttatgggga aacagacaat atgatccatt tcggaaccgg cgggtggcga gccattatag
     3001 gagatgaatt tacaaaaagt aacatccagc tgctttcata ttcaatcggt gatcttatgg
     3061 aaaaaaccgg tacagccaat ttgggcttat gtatcggata tgaccgccgc ttcttatcga
     3121 aagaagcagc ccattgggct gccgaagtct tcgcagcgcg acaaattcct tgttatgtga
     3181 tcgatcgttc atcccctacg cctttgatca tgtttacggt caaacaaatg aatttaccct
     3241 acgggatgac tattacggca agtcataatc ctgctattta taacggtgtg aaatttttta
     3301 ccgccggtgg atgcgatgca aatgaagatg ttacaaatga aattgaattc ttgacgcaga
     3361 aaaatgccga agtaaaaatt ccgtttcttg attacgatga gggaatccga tccggaatca
     3421 tcaaagaaat aaatccgctg aatagctata tcgatagtat cctttccttg gtagataccg
     3481 aagcgataaa aaaagccaga ttgagaatcg cattggaccc catgtatggc gtaagccaaa
     3541 ccagtttaca gaccatctta ttaaccgctc gttgcgaagt ggaagtgatt catgatcgac
     3601 atgatacgct gtttggcggt cgacttcccg ctccgaatat tcatacgctc gggacattgg
     3661 ttaatttcgt tttggatcat cattgtgatt tgggcattgc aacagacggt gatgccgatc
     3721 gcctgggagt aatcgatgat aaaggacaat ttgttcatcc gaataaactt ttggtcctgc
     3781 tttactatta tttgttgaaa tatcgcggtt ggcgaggtcc tgccgttcga aataattcta
     3841 caacccactt attggataaa gttgcggctg gattcggtca agcctgttat gaagttccgg
     3901 tcgggttcaa atatgtttcc gcaaaaatgg aggaaaccgg tgcgattatc ggcggtgaat
     3961 catccggggg tttaaccgtc aaaggacata tccgcgggaa agacggtatc tatgccgcca
     4021 cgattttaac agaaatggtc gcggttaccg gaaagcattt atccgaattg tacaaagaaa
     4081 tcaccgatca gtttggtgaa gtatttaatt cggatgccga ttttaaattg actccgcaga
     4141 ataaagaaag gttgcaaaat attttgtacg tcgaaaataa attaccggat tttccgtacg
     4201 aaatcgatca tgtttcttac cgagacggtt gtaaggtata tttcaaaaat tcaggctggg
     4261 ttattgttcg tttttcggga acggaaccct tgattcgctt atgcgcggaa atgtccgatc
     4321 aaaaatccgc tgaccaattg atttctgtct tttcagattt tttaggattg gaatcggaat
     4381 tgcaatctca ataatcgaga attctgaatc agccgaatcg atttctttat ttaacttgat
     4441 ttcgataatc gtttgcactg atccccacca tttttttaaa tagtcgtgag aaatgcccgt
     4501 tatcggtata tcctaccatt tcagaaatat cacttatacg caatttagga tcttccaata
     4561 atcgctttgc cgcgtcaatt cgggctttat tcaataaatc atagaaactt tgaccggtca
     4621 ctttgtttaa caattttgat aaatgccatt gggagacata acaataatcc gcgacatcct
     4681 gaagtgtcaa tttccgagaa aaattctctt cgatgtaggc taaagcttgt ctgacgatga
     4741 agctgtttgc ggaagcggtg cttaaatcat cgattttgtc gtctgatgcg gcaggagatt
     4801 gtcttgcgag tgtatcggtc ataaattgta aagcttcgtt aatttcactc atacgcgacg
//
LOCUS       DAOMIV010000096         4823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_17954_length_4823_cov_4.747273, whole genome shotgun sequence.
ACCESSION   DAOMIV010000096 DAOMIV010000000
VERSION     DAOMIV010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17954_length_4823_cov_4.747273"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKV59_03405"
     CDS             <1..196
                     /locus_tag="PKV59_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45831.1"
                     /translation="IEFLVETGTDIEFGARPLKRAIQHELQDPLAMEILNGKYVQGDT
                     IQVDLAPEGDHLVFSKKQTS"
     gene            354..983
                     /gene="upp"
                     /locus_tag="PKV59_03410"
     CDS             354..983
                     /gene="upp"
                     /locus_tag="PKV59_03410"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQP45832.1"
                     /translation="MSNVTITNHPLIEHKLSILRDVHTNPTVFRHLVNELGALLCYEA
                     TRDLPLCPVEVQTPLSMTTAYEMKESIALIPILRAGLGMVEGIWDLIPNAHVFHIGFY
                     RNEKTLQPVEYYNKLPKDHPFNISFILDPMLATGGSAIATTNIVKKWGAQTIKFVGII
                     GAPEGIKALQEAHPDVDIHLAAIDDHLNDKGYIVPGLGDAGDRQFGTVF"
     gene            complement(990..1541)
                     /locus_tag="PKV59_03415"
     CDS             complement(990..1541)
                     /locus_tag="PKV59_03415"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQP45833.1"
                     /translation="MYSDKLALFPKTILTQRLQLRPITRADENDIFEYASNPEVARYM
                     SWSAHRTLADTEEFLKYIDQVESEKLQIDRGIVWRENNKMVGTIAFVSIDLALRVVEL
                     GYCLSNQYWGRGIIVEAANAMIDAAVRTLDIHRIEAECEIDNFKSERVMQKLGMEQEG
                     VMRKRLPVHGVYCDAKLYAKVVG"
     gene            1817..2413
                     /locus_tag="PKV59_03420"
     CDS             1817..2413
                     /locus_tag="PKV59_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQP45834.1"
                     /translation="MTNPVITIGPRATLETAKDLIDKKSIHSLPVVDRQGRLMGLITK
                     DRLEDFIQKAKQADEPMPLVEDVMVQQVLTVTENTPIEEAARIISDYDVKSLPVVRGN
                     FVVGIITASSLIRIFLELTGARDQGVRLTIMVEDVPGKILAVIQKIVELNGNISTFST
                     YYSDEKNYRIMTLKISGVEKYKLKQEIAPIVYKIIDIR"
     gene            2623..>4823
                     /locus_tag="PKV59_03425"
     CDS             2623..>4823
                     /locus_tag="PKV59_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQP45835.1"
                     /translation="MIALDIETTGLDPNLDAVTEIAALRFKGARVEDTFQTLVNPNRP
                     IPSNITQLTHITNEMVRNAPQIGEVIGKVAQFVGNDIIVGHNISFDLNFLRLKGILQK
                     NLSYDTYDLASILLPDAPRYNLGVLAQRMGIIVRDEHRALADCYTTYQVFNRLIERAE
                     SLPAELLADIVNLSEGIHWNEAELFRQIIQKRIQNGEKIKPIYKITFPTFLNESGNNV
                     DPLKPSENPIPLEEDEMAAILEYGGAFSKYFPNYEQRSQQIEMLRTVTTAFSKGKHMF
                     IEAGTGTGKSFAYLIPAFHWAKQNGERVIVSTNTINLQEQLIRKDIPELNNVLQEDFR
                     AAVLKGRSNYLCPRRVQMLHSHGVENADELRVLSKVLVWLYEGGKGDRGEINLNGPID
                     REIWERLNADFEGCRPNQCPHYRDGQCPFYAAKAKALKSHVIIVNHALLLTDMAYGND
                     VLPEYKYLIIDEGHHLESAATGALSVRITQTDLSHQLQELGGYGRGLLGRMDNKLAEK
                     LKPDEYQLFVNATTFISENTALAEEALKSLFETFQFFMKEAREGNPVTVYGQQLRITS
                     AIRTLAGWADIEIIWDKTEKYIRELIDSAEKIDRSLMNLDDIDEELRELIDSFHGGLK
                     KLSESKKIVNNLIVKPDPETIYWIEISPVNNRLSLNSAPLHVGNLIEKMLWHEKESII
                     VTSATLTADNSFDYIRDRLSADEAEELVVGSPFDYENSVLLFIPNDIPDPN"
BASE COUNT         1447 a         1026 c         1014 g         1336 t
ORIGIN      
        1 gatcgagttt cttgtcgaaa cagggacaga tatcgaattc ggtgcccgtc ctttgaaacg
       61 tgcgatacaa catgaattgc aggatccttt ggcaatggaa attctgaacg gaaaatatgt
      121 tcagggtgac acaattcaag tggatttagc acccgagggc gatcatctgg ttttttcaaa
      181 aaagcagacc tcataataag atcaacaaaa aaaccgacca aagaatgaaa ttggtcggtt
      241 tttttataat tattttcctt tatgggttac ttttataagg tcaagaataa atgatcgctt
      301 tgtttccacc cctgttataa ttgtttccga tctaaaccta acggaggctt tttatgtcca
      361 acgtaacgat tacaaaccac ccattgatcg aacataaact ctcgatattg cgtgatgttc
      421 acaccaaccc gacggttttt cgccatttgg taaatgaatt gggcgctttg ttgtgctatg
      481 aggcaactcg tgatttgccg ctttgcccgg tagaggttca aaccccctta tccatgacca
      541 cagcttatga aatgaaagaa agcatcgcat taatccccat ccttcgcgcc ggactcggaa
      601 tggtcgaggg catttgggac ttaatcccaa acgcccatgt ttttcacatc gggttttatc
      661 gcaatgaaaa gaccttacaa cccgttgaat actacaacaa gctgcccaag gatcatccgt
      721 tcaatatatc tttcatcctc gatccgatgc ttgccaccgg cggttcagcc atcgcgacca
      781 cgaatatcgt gaaaaaatgg ggtgcacaaa ccattaagtt tgtcggaatt atcggagcgc
      841 cggaaggaat caaagcactt caggaagctc atcccgatgt ggatattcac ttggcggcaa
      901 tcgacgatca tttgaatgat aagggatata ttgtcccggg gttgggagac gccggcgatc
      961 gccaattcgg tacggtcttt taataaagat taaccgacaa ccttcgcgta gagtttggca
     1021 tcacaataaa ccccatgaac agggaggcgc tttcgcatta caccttcctg ctccatcccc
     1081 aatttttgca ttaccctttc ggatttaaaa ttatcaattt cacattcggc ttcgattcgg
     1141 tgaatatcca acgttcgaac cgccgcatca atcatggcgt tcgcagcttc tacgataatt
     1201 cctctccccc aatattggtt ggaaaggcaa taaccgagtt caaccaccct taaagctaag
     1261 tcaatcgaaa caaaggcaat cgtccctacc attttgttat tttcacgcca gacaatccct
     1321 cggtctattt gaagtttttc ggattcaacc tgatcgatat actttaaaaa ttcttctgta
     1381 tcagctaatg tacgatgagc tgaccaagac atataacgtg ctacttcagg attggaggca
     1441 tattcaaaaa tatcattctc atccgcccgc gtaataggcc gaagctgtaa ccgttgggtc
     1501 agaatggttt tgggaaataa tgccaattta tcagaataca taaattcctc ttatttcatt
     1561 aaagtcgaaa tgacgtatta taaaaacagc gtattgaagt atgtttcaat acgctgtagc
     1621 gataacttgc gatgtcacaa ggatttatta taaaagaaat aaacatgcta aaatagtgga
     1681 acgtcatata atccttatga cttttgttat atcatttcgt gacaacttgt tgtattttac
     1741 caccgttgcc aaaatatgac actcaaaaaa ataaaactga ttggagaaaa atcgaaaaat
     1801 gttggtcggc gctcgaatga caaatcctgt aattacaatt ggaccgcgag ctacgttgga
     1861 gacagccaaa gatctgattg acaaaaaatc gattcattcc cttcccgttg tagatcgtca
     1921 gggcaggctg atgggattaa ttaccaaaga cagattggaa gattttattc aaaaagccaa
     1981 gcaagccgat gaacctatgc cgttggtgga agatgtcatg gttcagcaag tattgaccgt
     2041 tacggaaaac acccccatag aggaagcggc acggatcatc agtgattatg acgttaaatc
     2101 cttgccggtc gttcgcggta attttgtcgt cggaattatt acagcgtcct ctctgattcg
     2161 tatctttctt gaacttaccg gcgcccgcga tcaaggggtg cgtttgacta taatggttga
     2221 agacgtcccc ggaaagattt tggcggtgat tcaaaaaatc gtcgaattga acggaaatat
     2281 ttctactttt tccacctatt attcggatga gaaaaattac cgcatcatga ctctgaaaat
     2341 cagcggcgta gaaaaatata aattaaaaca agaaatcgct cccattgttt ataaaataat
     2401 cgatattcgc taaaagcgac tgatttttgg tctcttttat ttattatgga ctttataagg
     2461 tcttatttta tccttttatt aagtacggaa aaaaatgcaa tccaaggttt ctcgaaggta
     2521 taatttgaat aagcgatgaa tcatcgcgca tcaacagccg atacaacata attttttgaa
     2581 tcgggagttg caaaattcgg attagaaagc agaaacaaat gaatgattgc tctggatatt
     2641 gaaaccaccg ggttagaccc caacctggat gcggttacgg aaattgccgc attacgtttt
     2701 aaaggggctc gggtggaaga tacttttcaa actcttgtaa atccgaaccg gccgattcct
     2761 tccaatatta cccaattgac ccatattacc aatgaaatgg tccgtaacgc accgcaaatc
     2821 ggtgaagtga tcggaaaagt cgcacaattc gttgggaatg atatcattgt cgggcataat
     2881 atcagtttcg acttgaattt cctccggctg aaaggaattt tacaaaaaaa cctttcatat
     2941 gatacatatg atttggcatc catcctgctt cccgatgcac cccgttataa tctcggcgtt
     3001 ttggcgcaga gaatgggcat tatcgtccgt gatgagcacc gagcgttggc ggattgttat
     3061 accacctatc aggtattcaa tcgtttgatc gagagagccg aatctttacc ggcagaactg
     3121 ttggcggata tcgtaaatct ttccgaagga atacattgga atgaagcgga attatttcgc
     3181 caaatcattc aaaaacggat tcaaaacggc gaaaaaatca aaccgattta taaaataact
     3241 tttccgacct tcctgaatga gagcgggaac aacgtcgatc ctctgaagcc ttcggaaaat
     3301 ccgatacccc tcgaagaaga tgaaatggcc gcaattcttg aatacggcgg agcattcagt
     3361 aaatatttcc cgaattatga acaacgatct caacagattg agatgctgcg aacggttacc
     3421 actgcttttt ccaaagggaa acacatgttt attgaagccg ggaccggaac cggcaaatca
     3481 tttgcctatt taatcccggc atttcattgg gcaaaacaaa atggtgaaag ggttattgtt
     3541 tctacgaata cgataaacct tcaggaacaa ttgatccgaa aagatattcc cgaactgaac
     3601 aatgtccttc aggaggactt ccgtgcagct gtcttaaaag gacgttccaa ctatctttgc
     3661 ccccgaagag ttcagatgct tcattcgcat ggggtggaaa atgcggatga attacgtgta
     3721 ctttccaagg tcttggtttg gttatatgaa ggcgggaaag gcgaccgcgg cgagattaac
     3781 ttgaacggcc cgattgatcg cgaaatctgg gaacgcttga acgccgattt tgaaggatgc
     3841 cgtcccaatc aatgccccca ttaccgtgac ggacaatgtc cgttctatgc agccaaagcc
     3901 aaagctttaa aatcgcatgt cattattgtt aatcatgcac tccttttgac ggatatggca
     3961 tacggaaacg atgttttacc ggaatacaag taccttatta ttgatgaagg gcatcatctt
     4021 gaatcagccg ctaccggagc attgagcgtc cgtataaccc aaacggattt atcccatcaa
     4081 ctgcaggaac tcggcggtta cggacggggc cttttgggaa gaatggataa taaacttgcg
     4141 gagaaattaa agccggacga atatcaattg tttgtgaacg cgacaacctt tatttcggaa
     4201 aataccgctt tggctgaaga agctctcaaa tcattatttg agacttttca gttttttatg
     4261 aaagaagccc gtgaagggaa ccccgttacg gtttacggac agcaattgcg gattacttcg
     4321 gctatacgta cgcttgcggg atgggctgat attgaaatta tttgggacaa aaccgaaaaa
     4381 tatatccggg aattaattga ttccgcggaa aaaattgatc gttccctgat gaatttggat
     4441 gatatcgatg aagaacttcg agaactgatc gactcgttcc acggtggatt gaaaaaatta
     4501 tcggaatcca aaaagatagt aaataatctg attgttaaac cggatccgga aacgatttat
     4561 tggatcgaaa tatcgcccgt gaacaaccgg ttatcgttaa attccgcccc tctgcatgtc
     4621 ggaaatttaa tcgaaaaaat gctttggcat gaaaaagaaa gcatcattgt cacctctgcg
     4681 acgctgacgg ctgataattc ttttgattat attcgcgacc ggttgtccgc tgacgaagcg
     4741 gaagaattag tcgtcggctc acccttcgat tatgagaact ccgttttgct ttttattccc
     4801 aatgacattc ccgatcccaa tca
//
LOCUS       DAOMIV010000097         4793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_18131_length_4793_cov_5.696285, whole genome shotgun sequence.
ACCESSION   DAOMIV010000097 DAOMIV010000000
VERSION     DAOMIV010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18131_length_4793_cov_5.696285"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PKV59_03430"
     CDS             complement(<1..920)
                     /locus_tag="PKV59_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP45836.1"
                     /translation="MILLSMENIQKKFFGKYANKNVHLQVEKGKILALLGENGAGKTT
                     LMNILYGLYQPDGGKIFWHGEEVKFESPKDAIERKIGMVHQHFMLVPTLNVSQNITLG
                     LKETGYPLTDRKAVNRKIKSLSDQYGLIVDPEAMVSDLSVGEQQRVEIMKLLYRDAEL
                     LILDEPTAVLTRQETIGLFEVLKRLSQDGRSVILITHKIPEVMEIADMVTVMRDAESI
                     ITVPITEVDEASLAEYMIGRPLNIVEIPPRIEKHDQGFILRNLNLEEKSQAKSATVSF
                     EIEAGEILGIAGVEGNGQKELAESILGIRH"
     gene            complement(986..2101)
                     /locus_tag="PKV59_03435"
     CDS             complement(986..2101)
                     /locus_tag="PKV59_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP45837.1"
                     /translation="MKKSLSILLFVVLIGSMLISGCSSGAKSTPTTAAVLTSTETTEV
                     KSEKEATSSVIPSAESKTVETGSGAKKLKVALILSGPANDQGWNATALEGLQAAEKEF
                     GVETSKMENVDIADSEAAFRDYAAQGFDLIIGHGYQFGEPAKKVSADFPNSYFVATEA
                     NSQSENMASYVMSCEQAAYLMGMLCAGMSKTKVIGVVGGFEQPSITKELEAFKLGAKE
                     VVPDIKVLEVYINSFVDASLGKEAAMSMIRQNADVLYHVANQAGTGVIMAAQENGLLA
                     CGNSYDQSSIAPDTVMASTVYRMPKVITTAVNQVKTGTFKGGVYHLGFADDVVDITGY
                     GKFEDKIPQDLKDKIAAKKEAIRTGEFVVPVIEKPTK"
     gene            complement(2126..3520)
                     /locus_tag="PKV59_03440"
     CDS             complement(2126..3520)
                     /locus_tag="PKV59_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQP45838.1"
                     /translation="MDEKILIRNAESIVSCDENDTVYHDADILIENGFIKEIGKNIST
                     DDSKIIHAEHKIIYPGLINTHHHFFQTFVRNLITIDYPNMLVVEWLDKIYRIFQMINS
                     DVIYYSSLAAMADLLKHGCTCAFDHQYCYTKATGKSPVDRQMEAAELLGIRYHAGRGT
                     NTLPRSEGSTIPENMLETTDEYLKDCERIIDLYHDPQPGSMHQIVLAPCQPINSYKET
                     FTETIALAREKGVRMHTHLGEGENEIMLQRWGKRSLAWCEEIGFVGPDVWYAHFWEGL
                     PEEDAVLAKYGCGVSHCPAPAILGGFPILDIKRMQQSGITISLGCDGSATNDSSNLLD
                     SMRIGYLMQTWHSKQRNGCISAYDMLKIATVSGAKTLGRSDLGSLEKGKCADLFMVDT
                     DVPELIGTNHDHKNILARVGLTGPVWLTMVAGKIVFEEGHLVGVDESKLARRGEEVCD
                     RVIREPFKEFFIVS"
     gene            complement(3741..3857)
                     /gene="rrf"
                     /locus_tag="PKV59_03445"
     rRNA            complement(3741..3857)
                     /gene="rrf"
                     /locus_tag="PKV59_03445"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(3922..>4793)
                     /locus_tag="PKV59_03450"
     rRNA            complement(3922..>4793)
                     /locus_tag="PKV59_03450"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1205 a         1254 c          899 g         1435 t
ORIGIN      
        1 ttgtgccgaa ttcccaatat cgattcggcc aactctttct gtccattccc ttcgacaccg
       61 gcaatcccca atatctcgcc cgcttctatt tcaaacgaaa ccgtcgctga ctttgcttgg
      121 ctcttttcct caagatttaa gttccgcaga ataaagcctt gatcatgttt ttcaattctc
      181 ggaggtattt caacaatatt cagaggtcgc ccgatcatat attcagctaa acttgcttcg
      241 tcgacttctg ttatcggaac ggtaataata ctttcagcat ccctcatcac cgtaaccatg
      301 tcggcgattt ccattacttc cggtatttta tgagtgatca gaatgacgga acgaccgtct
      361 tgagagagcc tctttaatac ctcgaacaaa ccaattgtct cttgtcgcgt caaaaccgcg
      421 gtcggctcat ccaaaatcag caactcggca tctcgataaa gcaacttcat gatttcaaca
      481 cgctgttgtt cgccgacgga taaatcgctg accatcgcct ccggatcgac gattaatccg
      541 tactgatccg ataaactttt tatcttccga ttaactgctt tccgatcggt taatggatag
      601 ccggtctctt ttaatccgag agttatattt tgagatacat tgagagtggg caccagcata
      661 aaatgctggt gcaccattcc gatttttctt tcgatcgcat ctttgggaga ttcaaacttt
      721 acttcttcgc cgtgccaaaa aattttaccg ccatccggtt ggtaaagacc gtagagaata
      781 ttcatcaagg ttgtctttcc ggcgccgttt tcgccgagta aagcaagaat cttccccttt
      841 tctacctgaa gatgaacatt tttgttggca tactttccaa agaatttttt ctggatattt
      901 tccatagaca gcaatatcat tttttcctcg acttttaccg tcatctgttc actcaggcaa
      961 gaactatttc ttcaaatctc ataacctatt ttgtgggttt ttcgataacc ggaaccacaa
     1021 attcaccggt tcgaatagct tcttttttcg cggcgatttt atcttttaaa tcctggggaa
     1081 ttttatcttc gaatttaccg taaccggtta tatccaccac atcatccgcg aaacccaaat
     1141 gatatactcc gcctttaaag gtccctgttt ttacttgatt cacagcggtg gttattactt
     1201 tcggcattct gtaaaccgtc gatgccataa ccgtatccgg agcaatactg ctttgatcat
     1261 atgaattacc acaggccaaa agaccatttt cctgtgcagc cataatcaca ccggtgccgg
     1321 cttggtttgc aacatgatac aaaacatctg cattttggcg gatcatgctc atcgcagctt
     1381 cttttcccag cgaagcatcg acaaatgaat tgatgtaaac ttcaagaact ttgatatcgg
     1441 gaacgacttc ctttgcaccc aatttaaacg cttccaattc cttggttatg gaaggttgct
     1501 cgaaaccgcc gacgacaccg attactttgg ttttactcat cccggcacaa agcatgccca
     1561 ttaaatatgc cgcttgttca caactcatca cataagaagc catgttttcg gattgtgaat
     1621 ttgcttccgt ggcgacaaaa taggaattcg gaaagtctgc ggatactttt ttggccggtt
     1681 caccgaattg atagccgtga ccgatgatca aatcaaaccc ctgagcggca taatctcgaa
     1741 aagctgcttc actgtcagct atgtcgacat tttccatctt tgaggtttca actccgaatt
     1801 ctttttcggc tgcctgcaag ccttccaatg cggttgcatt ccaaccttgg tcatttgcgg
     1861 gaccggacag aattagtgca actttcaatt tcttcgcacc gcttcccgtt tcgactgttt
     1921 tgctctcagc ggacgggata acagatgacg tcgcttcttt ttcggatttg acttcggtag
     1981 tctctgtcga agttaggact gcggctgttg tcggtgtact ctttgctccc gaggaacaac
     2041 ctgaaataag catactgcca atcaatacga caaataacaa aattgataaa cttttcttca
     2101 tttctccctc tcatctaata aacaattacg aaactataaa gaattcttta aacggttcac
     2161 gaattacccg atcacatacc tcctctcctc tccgcgcaag ttttgattca tccacaccca
     2221 ccagatgacc ttcttcaaat acaatctttc cggctaccat ggtgagccat accggtccgg
     2281 tcaatccgac cctcgccaat atatttttat ggtcatgatt tgtcccgatt aattcgggga
     2341 catcggtatc aaccataaat aaatcagcgc attttccttt ttctaaactc ccgagatcgc
     2401 ttcgcccgag cgttttagcc cctgaaaccg tcgcgatttt cagcatgtca taagctgaga
     2461 tacatccgtt cctctgtttg ctgtgccagg tctgcatcaa atatccgatt cgcatggaat
     2521 ccaataaatt ggaggaatca tttgtcgcgg atccgtcaca accgaggctg attgttattc
     2581 cggactgttg cattcttttt atgtccaaaa tggggaagcc gcctaaaatc gccggcgccg
     2641 gacaatgcga gacaccgcat ccgtattttg cgagaaccgc atcttcttcc ggtaaccctt
     2701 cccaaaagtg agcataccaa acatccgggc cgacaaatcc gatttcctcg caccacgcca
     2761 aagaacgttt cccccaacgt tgaagcatga tctcattttc cccttcaccc agatgagtat
     2821 gcatccgaac ccctttttcc cgtgccaaag caatcgtctc ggtaaaagtc tccttataag
     2881 aattgatcgg ctggcacgga gccaaaacaa tttggtgcat cgaacccggc tgaggatcat
     2941 gatataaatc gatgattcgt tcacaatctt ttaaatattc atctgtcgtt tccaacatat
     3001 tttccggaat cgtgctgcct tcgcttctcg ggagcgtatt tgtccctcgt ccggcatgat
     3061 aacggattcc caaaagttcg gctgcttcca tctgtctgtc cacgggtgat ttaccggtcg
     3121 cttttgtgta acaatactga tgatcaaatg cacaggtaca accgtgcttt aacaaatcag
     3181 ccatagctgc caaagaacta taataaatga cgtcggaatt aatcatctga aaaatgcggt
     3241 aaattttatc caaccactct accacaagca tattgggata atcgatcgta attaaatttc
     3301 gaacaaatgt ttgaaaaaaa tgatgatggg tatttattaa tccgggataa ataattttgt
     3361 gttccgcatg aatgattttc gaatcgtctg ttgaaatatt ctttccgatt tcttttatga
     3421 atccgttttc gattaagata tctgcatcat gataaaccgt atcgttttcg tcacaggaga
     3481 caatcgactc ggcattacgg atcaggattt tttcatccat ataaatctcc tcagttttta
     3541 aaatgtcatt tttattcgca tgtaacttag tatttatgat tctacaacct atagttacta
     3601 taggtgcaag gattcatgta gttcaacaaa aaaactctta agagttttaa caactcttaa
     3661 gagttttcgt tatgtcgttt caattcaatt tatattgcgt gattcaaaga ttcaattcgc
     3721 cattaaaaca aaaagaatca tgttggcgat aacctactct cccaggaggt cgccctccaa
     3781 gtaccatcgg cgttgacgcg cttgacttcc gggttcgaga tgtttccggg tatacccacg
     3841 tccctttcgt caccaacatg actcttcttt ccgtctacag tatgtacctt caggtaggaa
     3901 aacctacctg ttccttacat cacgtcctaa gccttcgatc attagtaccg gtcagctcaa
     3961 tacatcactg tactttcact cccagcctat ctagcacgtc gtctccgtgc gatcttattc
     4021 attcctgatg agagaattta tctcgaagca agtttcccac ttagatgctt tcagcggtta
     4081 tctctgccaa acgtggctac tcagctgtgc aatcgacatt acaactgacc caccagaggt
     4141 ttgtccaccc cggtcctctc gtactagggg cagctcttct caattctctt tcgcccacag
     4201 cggatagaga ccgacctgtc tcacgacggt ctgaacccag ctcgcgtacc gctttaaccg
     4261 gcgaacagcc ggacccttgg gaccttgtac agccccagga tgcgatgagc cgacatcgag
     4321 gtgccgagcg aagtcgtcga tatgaactct tggacttcac cagcctgtta tccccggggt
     4381 agcttttatc cggtaagcca cggccattcc actctgtacc gtgggatcac taagccctac
     4441 tttcgtatct gttcgacgcg ttggtcttac agtcaagctc ccttctgcct ttacactctc
     4501 tgacaggttt ccattctgtc tgagggaacc tttgggcgcc tccgttaccc ttttggaggc
     4561 gaccgcccca gtcaaactgc ccgcctgaca cggtcccttc tccagttctt ggattaaggt
     4621 tagagcccaa atatcatcag ggtggtattt caccgccgac tccaccaagg cttgcgccct
     4681 gatctctcag tctcccacct atcctacaca gataatacct aaactcaatg ccaggttaca
     4741 gtaaagctcc acggggtctt tttgtcctgc tgcgggtagg ccgcatcttc aca
//
LOCUS       DAOMIV010000098         4768 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_18248_length_4768_cov_4.723955, whole genome shotgun sequence.
ACCESSION   DAOMIV010000098 DAOMIV010000000
VERSION     DAOMIV010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18248_length_4768_cov_4.723955"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..1893
                     /locus_tag="PKV59_03455"
     CDS             370..1893
                     /locus_tag="PKV59_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQP45839.1"
                     /translation="MAVKPLSSTPSHPIQIISHDTVYSGSSEKGVCSWKGIPYAKSTA
                     GEGRFRPPQIPEKVDEIVADQFGPACPQHKHLNVPKNENCLNLNIWARFGAKRRPVLF
                     YIHGGSFMSGTGSDPVFDGQELVAAENIVVVTINYRLGVLGILDFSFLGDRFTPNCAI
                     LDVCMALRWTYEHIADFGGNPDNITVMGESAGGSIVSLLPTLPEAAQYISKCVISSGV
                     PTEFWTKSQAQQMAQAYMKFMGISSAEELLAISAERIGDSTQDFTQYTKLGAASYEPS
                     VDGKIIRDFPLVSVQQAEFKKIPMWIGMTRDEMSFLTFSILSRRWGLAKLMEEGTDEE
                     SAALREKIIKIYDETYDKDDARIQLYSDMVIRAPLIWYAEEASQTTHVWFYRMDWSSR
                     IMDKLGIKAFHSSDLPFFFGNFNFPANRIYYQGKEEKKIVKAIERSIQRDLATFMDSG
                     TLPWETATEGFIGKCYGDEIRYEAIMPKEIRDYWRQTTYHERSFISAWQSNNTGTNQ"
     gene            complement(1911..2525)
                     /locus_tag="PKV59_03460"
     CDS             complement(1911..2525)
                     /locus_tag="PKV59_03460"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HQP45840.1"
                     /translation="MEISINPRLNKRIQIALNVLKVLSGVLILIMAIYGYRNNLFTSQ
                     ENLTHFIKRFGAWGPFILTVYIAIQVIIPVLPGSLMLVVSPLLFGAFRGFLYSYIGIL
                     IGSVLAFLLAKRYGQVVLSIFVNEKYRQIFDKYTSKKQFTINFALAILLPGFPDDYLT
                     YLAGTTSMSLRTFLIIIVPCKIPGILLYSLGIPSLYKYISKHIF"
     gene            complement(2782..3441)
                     /locus_tag="PKV59_03465"
     CDS             complement(2782..3441)
                     /locus_tag="PKV59_03465"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HQP45841.1"
                     /translation="MNAIVVGGGNVGIYITRLLRENGNQVKIIDYNETITKKLEAEFG
                     KENVFLGNGSNPAVLERTGIEKADVLLAVTGADEANLAVGTLAKYEFGVRKVIGKVNN
                     PLNAWLYTKQFGIDMVINQAELISKIAMEQIDFEDMTILLRLSEEKYSIVQFEIKGNS
                     PSVGKCISDLGLPEESLVISISRNKKLQICKGNTILQAGDHLIALVKDSVNEEFKKHF
                     Q"
     gene            complement(3445..4107)
                     /locus_tag="PKV59_03470"
     CDS             complement(3445..4107)
                     /locus_tag="PKV59_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HQP45842.1"
                     /translation="MEKNNVIIIGCGRMGAGLALRISARGQDVTVIDSNPEAFWRLES
                     PKYTGGKIVGIGFDRDILIKAGIETAYGVVSCTSKDETNAVIGRMARLVFRVPVVIAR
                     LYDPLNVEIYNRLGIRAISTTTWGIDKALNLLSHNPLDTVMEFAHNDVSLIRVEANAM
                     MEGHTVHDFFRVAQVQVVVIERGNSSFIPTFGTKLEAHDVLFVCVDRSQINAFKHSIG
                     ME"
     gene            complement(4109..>4768)
                     /locus_tag="PKV59_03475"
     CDS             complement(4109..>4768)
                     /locus_tag="PKV59_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HQP45843.1"
                     /translation="KFLTYILNSNMPEAVPSAFFLFSRGLIPLPLTVMQILTIDLGTD
                     MTPALGLGTEPPEPGIMEKPPRKLSESLLNRNVIVKAFLWYGMLESVFAMAAYFFVNW
                     RNGWPALPLAADGFVYRQATTMTLAAIVFCQIGMVMNCRTDFESIFKVGLFKNKLVIR
                     GIIVEIGLILMIVYVPFLQRIFGTEPLFLSDWLFLSTLPFIIIAIEEIRKALIRKKRK
                     A"
BASE COUNT         1373 a         1005 c         1054 g         1336 t
ORIGIN      
        1 ggtcctttca gaaaaatccg attgtttggt aaatgagaag agacttttgg caagcctgga
       61 agggggtagg caagagggct tccctgagaa ttgttgccta tccttacaac aattcgaatg
      121 tctgaaataa ggatttcaga cgcttgaggt cctttcagaa aaatccgatt gtttggtaaa
      181 tgagaagaga cttttggcaa gcttggaagg gaaagaaaga ggaatgtcga gtcaagttga
      241 caatcacgat ttataaggct tccgcaagga tttgatattt ggtaaaatta tcctaaccgt
      301 ttggttaaat tgtttccgaa aacggatcgt gtcaaaaccg gatatttatt cagaaatcag
      361 cgctatctta tggctgtcaa accactcagt tcaaccccat ctcaccctat tcaaattatc
      421 agtcacgata cggtttatag cggcagttcg gagaaaggag tatgctcttg gaaaggaatt
      481 ccgtatgcga aatcaacggc gggagaaggc cgtttccgtc cgcctcagat tcccgaaaaa
      541 gtcgatgaaa tcgttgcgga tcagttcggt ccggcttgcc cgcagcacaa acatttaaat
      601 gttcctaaaa atgaaaattg tctgaacttg aatatctggg cgcggttcgg tgcaaagagg
      661 cggccggttt tgttttatat tcacgggggt tcatttatga gcggaaccgg ttctgatccg
      721 gtctttgacg gacaagaatt ggttgcggcg gaaaatattg tcgtggtgac gattaattat
      781 cgcctgggtg tgttgggcat cctcgatttt tcatttttgg gtgatcgttt tactcccaat
      841 tgcgcaatcc ttgatgtttg tatggcgctt cgttggactt atgaacatat cgccgatttc
      901 ggcggaaatc cggataatat aacggttatg ggcgaatctg ccggcgggag catcgtaagt
      961 ctgcttccta cgttacccga agcggcacaa tatatttcca agtgcgttat ttcaagcggg
     1021 gttccgacgg agttttggac gaaaagccaa gctcaacaaa tggcacaagc ttatatgaaa
     1081 tttatgggga tctcttccgc agaagaactg ctggcaatct ctgccgaacg gatcggagat
     1141 tccacacagg attttacaca atataccaaa ttgggtgcgg catcgtatga accgagcgtt
     1201 gacgggaaaa ttatacggga ttttccgctt gtatctgttc agcaagccga gtttaaaaaa
     1261 atacccatgt ggattggtat gactcgggac gaaatgtctt ttttgacatt ttccatcctt
     1321 tcccgccgtt gggggctggc aaaactcatg gaagagggga cagatgagga gtcggctgcg
     1381 cttcgagaaa agattataaa gatttatgac gaaacttatg ataaggatga cgcaagaatt
     1441 cagttatatt cggacatggt gattcgcgca ccgttaattt ggtatgccga agaagcctct
     1501 caaaccactc atgtctggtt ctatcgaatg gattggagca gtcgaataat ggataaactc
     1561 ggtataaagg catttcactc ttccgattta ccgtttttct ttggtaattt taatttcccg
     1621 gctaaccgaa tatattatca ggggaaagaa gaaaaaaaga ttgtaaaagc gatcgagcgc
     1681 agcattcaaa gggatttggc tacttttatg gattccggaa cactaccctg ggaaactgca
     1741 acggagggat ttatcgggaa gtgttacggt gatgagatcc gctatgaagc gataatgcca
     1801 aaagaaattc gtgattattg gaggcaaaca acttatcacg aacgatcttt tatttccgcg
     1861 tggcagtcaa acaacacggg aacaaatcaa taaattcctt gaatttagaa ttaaaagatg
     1921 tgctttgaaa tatatttata taaggacggg atccctaaac tataaagaag gatgcccggg
     1981 attttgcacg gtacgatgat gatcaaaaaa gttcttaatg acatggaagt cgtaccggcc
     2041 aggtaagtca gatagtcatc cggaaacccg ggaagaagta tagccagcgc aaaattgatt
     2101 gtaaattgtt ttttgctggt gtatttatcg aaaatctgcc ggtatttttc atttacgaaa
     2161 atggaaagaa cgacttgccc gtatcgcttt gcgagcaaaa aagcgagaac ggaaccgatt
     2221 aagattccaa tatagctata gagaaaacct ctaaaagcac cgaataacag agggctcaca
     2281 accagcataa ggctgccggg caaaacaggg atgataacct gtatcgcgat atagacggtc
     2341 aaaataaacg gtccccaggc gccgaaccgc ttgataaaat gagtcaaatt ttcctgtgaa
     2401 gtgaacagat tatttcggta gccatagatt gccatgatta aaataagaac acctgaaagc
     2461 accttgagca cattcaatgc aatctgaatt cttttattta atctcgggtt gatagaaatt
     2521 tccatcggcc ttatccgttt ccttgtttta tttttatcat agaatttgaa aaaaaccata
     2581 ccttttcaaa acttaatctt ccgaaactta tgattgtcaa tgaatctgct ccttgtagaa
     2641 tcgatatacg tttttccgcg gagacttgtt gcagaagaaa gattaagatg atattaacga
     2701 aaaaaggaga ggaaaagagg gcatttttct ttaaaattgt tctgccctct ttatgatgac
     2761 cgaatatctt atcgtttact tttattggaa atgtttctta aattcttcat ttaccgaatc
     2821 tttaactaaa gcaatcaaat gatcgccggc ttgaaggatg gtattccctt tgcagatttg
     2881 tagtttttta ttgcgagaaa tcgaaatgac caaagattct tccggaaggc ctaaatccga
     2941 tatgcatttg ccgaccgaag gagaatttcc cttgatctcg aattgcacga tggagtattt
     3001 ctcttcgctc agccttaaaa gaatggtcat atcctcaaag tcaatttgtt ccatggcaat
     3061 cttgctgatc aattctgcct gattgataac catatcgata ccgaattgct tcgtataaag
     3121 ccaggcgttc aatgggttgt tgacttttcc gataactttt cgcaccccga attcatattt
     3181 tgccaaggtt ccgacggcaa gattggcttc atccgcaccg gtgaccgcca acagtacatc
     3241 cgctttttcg attcccgttc tttcaaggac cgccgggttg cttccgttgc ctaagaaaac
     3301 attttctttg ccgaattccg cttcgagctt ttttgtaatg gtctcattgt aatcaatgat
     3361 tttcacctga ttcccgtttt ccctgagcag acgggtaata tagataccga catttccgcc
     3421 acccacaaca attgcgttca tgtgctactc cattccgata ctgtgtttaa acgcattgat
     3481 ctgactgcga tcaacgcaaa cgaaaagcac atcgtgagct tctaacttcg tgccaaaagt
     3541 gggaataaac gaggagttcc cgcgttcaat gacaaccacc tgaacctgtg cgactctgaa
     3601 aaaatcatgg acggtgtgcc cttccatcat ggcgttggct tccacacgaa tcaaagaaac
     3661 atcgttatga gcgaattcca taacggtatc cagcggatta tggctgagca gattcagcgc
     3721 tttgtcaatt ccccaagttg tggtagaaat tgcacgaatt cccaaccggt tgtaaatttc
     3781 gacattcaac gggtcgtaca gacgggcaat tacgaccggt actcggaaaa ccaaccgtgc
     3841 catcctgccg atgaccgcat ttgtctcatc tttgctggtg caacttacga cgccgtaagc
     3901 tgtttcgatc cccgctttta ttaaaatatc gcggtcaaaa ccgattccga caatttttcc
     3961 gccggtgtat tttggggatt ccaatcgcca aaaagcttcc gggttcgaat caattaccgt
     4021 gacgtcttgg cccctggcag aaattcggag tgccaatccg gcacccattc ttccgcaacc
     4081 gataatgatg acattattct tttccatttt aggctttcct ctttttcctg ataagcgctt
     4141 tacgtatctc ttcgatcgca atgatgatga atggcaatgt ggataaaaac agccaatcac
     4201 tcaagaaaag cggttctgtt ccgaaaattc tctgcaaaaa aggaacataa acaatcatta
     4261 aaatgagtcc gatttcaaca ataataccgc gaatcactaa tttgtttttg aataagccga
     4321 ctttgaaaat cgattcgaag tcggtccggc aattcattac cataccaatc tggcaaaata
     4381 caatcgctgc caaggtcatt gtggtggctt gtcgataaac aaacccgtct gcggcaagcg
     4441 gaagtgccgg ccaaccgtta cgccaattga caaagaaata ggctgccatc gcaaaaacag
     4501 attccaacat tccataccac aaaaatgctt ttacgatcac gttgcgattc aacaaacttt
     4561 cactcaattt tcggggcggt ttttccataa ttccgggttc gggaggttcc gttcccaacc
     4621 ccagtgcggg tgtcatatcc gttcccagat cgatcgttaa gatctgcatg acggtcagcg
     4681 gcaaggggat taatccgcga gagaacaaga aaaaggcgga tggtacagct tcgggcatgt
     4741 tactgtttag gatatacgtc aggaattt
//
LOCUS       DAOMIV010000099         4760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_18283_length_4760_cov_4.514559, whole genome shotgun sequence.
ACCESSION   DAOMIV010000099 DAOMIV010000000
VERSION     DAOMIV010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18283_length_4760_cov_4.514559"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="PKV59_03480"
     CDS             complement(<1..1800)
                     /locus_tag="PKV59_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45844.1"
                     /translation="MNTETTNRQSMPRHGGGRRGRMTGEKARDFRGTMIKLIKYLSVY
                     RNSILIVVLFAAISTVFTIIGPKILGQAITKLFEGSIAQLTGTGSIDFDAIGKILLTV
                     AGLYLTSAAFSYGQGWIMAGVSAKITYRFRKDIAEKINRMPLGYFESTNHGEILSRVT
                     NDVDTVSHTLNQSLSQIITSSITVIGVLIMMLSISWLMTLVALAIIPLSLLVVSLIVK
                     QSQKYFKQQQDYLGHVNGHVEEMYGGHIVIKAFNGEEKSIQRFNQLNDKLYEAAWKSQ
                     FFSGMMMPIINFIGNIGYVAVCILGGYLAVQKTITVGDIQAFIQYVRNFTQPITQLAN
                     ISNVLQQTAAAAERVFEFLNEAEESPETENPVDPETVRDRVEFKNVRFGYNPQKIIIK
                     NFSANVDSGQKIAIVGPTGAGKTTIVKLLMRFYDVTDGAILIDEHNVKDFYRKDLRQM
                     FGMVLQDTWMFNGTIIDNIRYGRPDVSDEEVIAAAKAAHIDHFIRTLPEGYNFILNEE
                     TTNISQGQMQLLTIARAILANPKMLILDEATSSVDTRTELLIQKAMDHLMANRTSFII
                     AHRLSTIRDADLILVMRDGDIVEQGKHKELLARG"
     gene            complement(1797..4043)
                     /locus_tag="PKV59_03485"
     CDS             complement(1797..4043)
                     /locus_tag="PKV59_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP45845.1"
                     /translation="MQRLLKYLKPFIFLLILAIALLFLQANADLALPDYLSKIVNVGI
                     QAGGIENAIPQAIRQTEMERLMLFFSAEEKERVLNDYTLVDQSSPDYNRYLSQYPALK
                     DGAIYVLNPIDQAETDWLNPVMAKALLVVYGIEQGMADPTKVTALGQMNFNGFDISKL
                     PPGTDLFKYMGQMPPAALSQISESINKRLEAMGSSSLIQAAAAPIQAEYAALGMDVGA
                     VSHKYILNTGKLMLLLTLLSVLCNIGVGFLSSRIASGFSRNLRKAVFSKVESFSSGEF
                     EKFSTASLITRTTNDITQIQMVVIMMVRMVFYAPIIGVGGVMRAINKDSSMWWILALA
                     VGILILLIIIIFNIAVPKFKIIQSLIDRLNLITRENLSGMMVIRAFNRQDFELKRFDK
                     ANRDLTSTSLFVNRVMAVMMPIMMLVLNGISLLIIWVGGKQVAASNMQVGDILAFMQY
                     GMQIIFAFLMLSVMFIILPRAQVSANRIADVLETEPSVVDPENPQSFPPDGKGLLEFR
                     NVSFRYPDAEEYILHDISFVAQPGKVTGIIGPTGSGKSTLVNLIPRFYDVTEGAILID
                     GVDIRNVTQHELRKRIGFVPQKSSLFSGTIESNLRFGNENAPEELLYKAAEIAQATEF
                     IEAKPEKMDAEISQGGTNVSGGQKQRLSIARALVKQAPINIFDDSFSALDFKTDAALR
                     QALKQQAVPGTLLLVTQRVASIMNADQIIVLDEGRIVGKGKHQDLMKTCEIYKDIALS
                     QLSMEELA"
     gene            complement(4124..4573)
                     /locus_tag="PKV59_03490"
     CDS             complement(4124..4573)
                     /locus_tag="PKV59_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQP45846.1"
                     /translation="MINQIHSLLSAFTTWTGLSMNYSMHGFVRFIRENGLSLSQISTL
                     FQLYHCGTSAVSQISSNLDISNAAVSQLLERLVQQGLITRSENPLDRREKQLVLTNKG
                     YELLRDSAKACSAWQEKLISTLTPEEQNKVSEALEILIDKTKQLPNA"
BASE COUNT         1335 a         1033 c         1007 g         1385 t
ORIGIN      
        1 tccgcgagcc aaaagttctt tgtgctttcc ctgctcaaca atatctccgt ctcgcataac
       61 taaaatcaaa tcagcgtcac gaattgttga aaggcgatgg gcaataataa aactggtgcg
      121 gtttgccatt aaatgatcca ttgctttttg aatcaacaat tcggtacgag tatcgaccga
      181 gctggttgct tcatccaaga tcagcatttt tggatttgcc aaaattgcgc gtgcaatggt
      241 taataattgc atttgtcctt gcgaaatatt tgttgtttcc tcatttaata taaaattata
      301 accctccggc agcgtacgaa tgaagtgatc gatatgagct gctttcgctg cggcaataac
      361 ttcctcatcg cttacatccg gccgaccgta ccggatatta tcgatgatgg ttccgttgaa
      421 catccaagtg tcttgtaaga ccattccaaa catttgccgg agatctttac ggtagaaatc
      481 cttcacatta tgttcgtcaa ttaaaatcgc cccatcggta acatcataga agcgcatcag
      541 taatttaaca attgtcgttt tccccgctcc cgtcggtcct acgatggcga tcttttgacc
      601 ggaatccaca tttgcggaaa agtttttgat gatgattttt tgcgggttgt aaccgaaccg
      661 aacgtttttg aattcaactc gatctctgac tgtttccgga tctaccggat tctctgtttc
      721 gggcgattct tccgcttcat ttaagaactc aaagacacgt tccgctgctg ccgctgtctg
      781 ttgcaggaca tttgaaatat ttgcgagctg tgtgatcggc tgggtaaagt ttcggacata
      841 ttgaataaat gcttgaatat caccgactgt aatggttttt tggaccgcca gatatccccc
      901 gaggatacaa acggctacgt aaccgatatt gccgataaaa ttaatgattg gcatcatcat
      961 tccggagaaa aattgtgatt tccatgcggc ttcatagagt ttatcgttca gttgattaaa
     1021 tctttgaatg ctcttttctt ccccattaaa agctttgatt acgatatgcc ccccatacat
     1081 ttcttccacg tgtccgttga catgacccaa ataatcttgt tgctgcttaa agtatttttg
     1141 agattgttta acaatcaggc tgacgaccaa caaggagaga gggatgattg ccaaagccac
     1201 caatgtcatc agccatgaaa tggatagcat cattatcagc acaccgataa ccgtaatcga
     1261 tgaagtgata atttgcgaca gactttggtt cagtgtatgg cttaccgtat cgacatcatt
     1321 ggtaactcgg gaaagaattt cgccgtgatt ggtgctttca aaataaccga gaggcattcg
     1381 gttaatcttt tcggcaatat cctttcggaa ccggtaggta atttttgcgg atactcccgc
     1441 cattatccaa ccctgaccgt agctgaaggc ggcagaggtt aaatatagtc ctgcgacggt
     1501 taaaagaatt ttaccgattg catcgaaatc aatacttccc gttccggtta attgggcgat
     1561 tgagccttca aataattttg taattgcttg gcctaatatt ttggggccga tgatggtaaa
     1621 tacggtggaa atagcggcaa acagaacaac aatcaaaatt gaatttcgat aaacactcag
     1681 atatttaatg agtttaatca tcgtgccgcg aaaatcacgg gctttttcac ctgtcatgcg
     1741 acctcttcga ccaccgccat ggcgtggcat agattgtctg tttgtggtct ctgtgttcat
     1801 gctagttcct ccatacttaa ttgcgataaa gcaatgtctt tgtagatttc acaagttttc
     1861 atcaggtctt ggtgttttcc tttaccgacg atacgtcctt catccaatac aatgatttga
     1921 tcagcattca taatgctggc cactcgttga gtcacaagca atagagtgcc gggtacggct
     1981 tgctgtttca gtgcctgtcg cagagcggca tccgtcttga aatccaaagc cgaaaagctg
     2041 tcatcaaaga tgttaatcgg tgcctgcttg actaaagcgc gagcaatcga taatcgttgt
     2101 ttctgtccac ctgatacgtt tgtgcctcct tgggagattt ccgcatccat cttttccggt
     2161 tttgcttcga taaattcagt tgcttgcgca atctctgcgg ccttatagag cagctcttcc
     2221 ggtgcattct catttccgaa acgcagattt gattcaatcg tgcccgaaaa taaagaactt
     2281 ttctggggga cgaaaccgat ccgtttacgc agctcatgct gagtcacgtt ccttatatcc
     2341 acgccgtcga tcagaatagc gccttcggta acgtcataaa aacgtgggat caggttcacc
     2401 aatgtcgatt tacccgaacc ggtcggaccg ataataccgg tgactttacc cggttgagcg
     2461 acgaaagaga tatcgtgtaa gatatattct tcggcatcag ggtaacgaaa cgagacattt
     2521 ctgaattcca acaaaccttt cccatcgggc gggaaagatt gcggattctc cggatcaacc
     2581 acagaaggtt ccgtttcaag gacatccgca atacggttgg cagatacttg agcccgcgga
     2641 agaatgataa acatcaccga aagcatgaga aaagcgaaaa tgatctgcat tccgtactgc
     2701 ataaaggcta aaatatcgcc tacctgcata tttgaagccg ccacctgttt cccacctacc
     2761 caaataatca gaagtgaaat tccgttcaaa accagcatca taatcggcat catgaccgcc
     2821 atgacgcggt tgacaaagag actggttgag gtcagatccc gattggcttt atcaaaacgt
     2881 ttcaactcaa agtcctgtcg gttgaaagcc cgaatcacca tcattccgga gagattctcc
     2941 cgcgtgatca gatttaagcg gtcaatcaaa ctttgaataa tcttgaattt tggaacggca
     3001 atattgaaaa taataataat taataaaatc aatataccca cggcaagcgc aaggatccac
     3061 cacatagaag agtccttgtt aatcgcacgc atcactcccc cgacaccgat gatcggtgca
     3121 tagaaaacca tccgtaccat cataataact accatttgaa tctgggtaat atcatttgtg
     3181 gttcgtgtaa tcaatgaagc tgtcgagaat ttttcaaatt ccccactgga aaaactttcc
     3241 acttttgaga aaacagcttt tcgtaaattt cgggagaatc cggaggcgat tcgtgacgaa
     3301 agaaatccaa cgccgatgtt acagagcacc gaaagaagtg tcaacaataa cattaatttt
     3361 ccggtattta gaatgtattt atgagaaaca gcgcctacat ccatccccaa ggccgcatat
     3421 tcggcttgga tcggtgccgc agctgcctga atcaagctgc tgcttcccat ggcttccaaa
     3481 cgcttattga tgctttcact aatttgcgaa agagctgccg gcggcatttg ccccatgtat
     3541 ttaaagagat cggttccggg cggaagtttt gaaatatcaa aaccattaaa attcatctgt
     3601 ccgagagcgg taactttggt aggatcagcc atcccctgct cgataccgta gaccaccaga
     3661 agcgctttcg ccatgaccgg atttaaccaa tccgtttcgg cttgatcgat cggattaaga
     3721 acataaattg caccgtcttt cagagcggga tattgggata ggtagcgatt gtaatccggt
     3781 gaagattggt ccaccagggt gtagtcattt aatacccgct ccttttcttc ggctgagaag
     3841 aaaagcatca aacgttccat ttccgtttgc cgaattgcct gcgggattgc gttctcaatt
     3901 cctccggcct gaatgccgac attgacgatt ttcgataagt aatcaggcaa tgccagatcg
     3961 gcgttcgctt gtaaaaaaag caatgcgatc gcaagaatga gcagaaaaat aaatggtttt
     4021 aaatatttta ataaacgttg catttagaac tccgttatta ggaaatcaag attagtgaag
     4081 aacgggagat atttttttga cataggtttt cataatcgga taattatgcg ttcggaagct
     4141 gtttcgtttt atctatgagg atttccagtg cttccgatac tttattttgt tcttcagggg
     4201 ttaaggtaga aataagtttt tcttgccatg cactgcaggc ttttgcacta tcccttagca
     4261 attcatatcc tttatttgtc aacacaagct gtttttcacg acggtcgaga ggattctctg
     4321 atcgtgtgat taaaccctgc tgaacaaggc gctcaagcag ttggctgaca gcggcatttg
     4381 aaatatctaa attggaactg atttgagaaa ccgccgaggt accgcaatga taaagttgaa
     4441 aaagggtcga aatttgagat aaggaaagtc cgttttcgcg gatgaatcgc acgaatccat
     4501 gcatggaata attcatcgac agacctgtcc aagtggtaaa agcggataac aaagaatgta
     4561 tttggttaat catacttaat ccttaagcga gcttatatat aagtcaagct caatgatttg
     4621 tcaagtatga gaaaacgggt ataagagata tttttgtgat taaataggaa agatgtttga
     4681 attgattcag agttgtcttg aattattgat ccttatttat cgcctctatt ataagaattt
     4741 gattgcaata aaggtgcttt
//
LOCUS       DAOMIV010000100         4654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_18891_length_4654_cov_4.907806, whole genome shotgun sequence.
ACCESSION   DAOMIV010000100 DAOMIV010000000
VERSION     DAOMIV010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18891_length_4654_cov_4.907806"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..775
                     /locus_tag="PKV59_03495"
     CDS             146..775
                     /locus_tag="PKV59_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQP45847.1"
                     /translation="MAKKSRDQKDNREIPEKDERAENTEINDENVGVDKNPDFSAPDG
                     CEEVLVLSKAEVDEMKEKLHRSQQKEKENLESWQRERADFINYKKRIDREQEILQQNY
                     KAELLKKYLVILDDLELAMRNKPEEGEDPTNWIRGIELILRKFESINQNEGLKKIEAE
                     GQEFDPRYHQAISHEEDPNVESGKIIEVLQNGYMLGDRVIRPALVRVAK"
     gene            787..2706
                     /gene="dnaK"
                     /locus_tag="PKV59_03500"
     CDS             787..2706
                     /gene="dnaK"
                     /locus_tag="PKV59_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQP45848.1"
                     /translation="MGKIIGIDLGTTNSVMAVMVGGEPTVIPTAEGERICPSVVAVNK
                     NHERLVGRVARNQAITNPENTIFSIKRFMGRKYDDPEVQKTLSRIPYKVTKAPNGDVR
                     VVLDGKEYSAPEISAMILAKMKADAEAYLGEKITQAVITVPAYFNDSQRNATKDAGKI
                     AGLEVLRIINEPTASSLAYGLDKKTNELIAVYDLGGGTFDISILDVGDGVFQVRSTSG
                     DTFLGGDDFDQRIMDYLIDEFKKANNMDLRQDRQALQRLKEAAEKAKIELSSTMQTEI
                     NLPYITADATGPKHLVMTLTRAKVEQLTSDLIARSLEPVRNALRDANLKTSDINEVVL
                     VGGMTRMPAVQEAVKREFGKEPNKSVNPDEVVAVGAAIQAGVLGGEVNDILLLDVTPL
                     TLSVETMGGVATPMIERNTTIPAKKTQIYSTAADSQTSVEIHVLQGERPMAADNKSLG
                     KFILDGIPPARRGVPQIEVTFDIDANGILKVTAVDKATNRSQNITISASSGLTDAEVE
                     RMRKEAEIHAEEDRKRKEGIEIKNNADNAVYTAEKLLEENGDKIPADMKSNIQSKAGE
                     LKNILNGTDFDTMKRMTDELNKMIQDIGNIMYQQQQPGAGQSQSDQGSGASGQNPPTG
                     NDGNDDVVDGEFRSM"
     gene            2841..3989
                     /gene="dnaJ"
                     /locus_tag="PKV59_03505"
     CDS             2841..3989
                     /gene="dnaJ"
                     /locus_tag="PKV59_03505"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQP45849.1"
                     /translation="MVKDRDYYEVLGVGRNASSDELKTAFRNLARKYHPDINKDPGAE
                     ETFKEINEAYQVLSDPDKRAAYDRYGKAGVSGVGNGFDYSQVDLSDILGDLFGFGGFG
                     GFGGFGSTQYSSRQRNAPRRGADLQTTVTLKFEEACFGVDKEVQFSRDEKCSRCHGTG
                     AEPGTSPQRCYTCNGTGEIKTNRQTMFGSMVQVSTCPTCNGRGEIISTPCTQCHGRGL
                     ERKTVKKVVSIPAGVDTGTRIRLSGEGQPGDNNGPSGDLYVDIRVNPHKYFHRKDADI
                     QLDMNINIAQATLGDEIDVPTIDGTEKLKIPAGTQPGRVFTLKGKGIPHLRGGGRGDQ
                     KVVINVEIPNSLTPEQRDLMEQLAGTMGTEVIPQEKSFWDRLKDALNG"
     gene            3982..>4654
                     /locus_tag="PKV59_03510"
     CDS             3982..>4654
                     /locus_tag="PKV59_03510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQP45850.1"
                     /translation="MADPAWLEISVVVDSETAEAVAEVLSRYAENGVVVEQGVDYNDA
                     EDVGTPFGPCRVTGYLPVDETTEEKRQRILEGLWYLGRITAVPEPEFKFIEDEDWMAA
                     WKKFYHPILIGEKLLILPAWMPSIEPERIAIKIDPSMAFGTGTHPTTQLCLAMVEKYI
                     VPNEDIIDMGCGSGILSIGALLLGARHALGVDIDNESMQSSRENAENNGVADRLAKEI
                     PGAYRP"
BASE COUNT         1410 a         1014 c         1241 g          989 t
ORIGIN      
        1 gatcggacca atgcgtatgc catacagccg aaacatcccg acggttcgat ttattgccgg
       61 attgctcagc gatcttgttt cggaatcgct cggataaaaa aaacctattc aagcggatag
      121 aataaaaaaa ttacagggaa tagatatggc gaaaaagagt agagatcaaa aagataacag
      181 agaaattccg gaaaaagatg agcgtgctga aaatactgaa atcaacgatg aaaacgttgg
      241 agttgacaaa aaccctgatt tttcagcacc cgacggctgc gaagaagtgc tcgtcctttc
      301 taaagccgaa gtggatgaga tgaaggaaaa gcttcatcgt tctcagcaaa aggaaaagga
      361 gaatctggag agttggcaaa gagagcgcgc agattttata aattataaaa aaaggattga
      421 ccgtgagcag gagatcttac agcaaaatta taaagctgaa ctgctgaaaa aatacttggt
      481 catcttagat gatttggaat tagctatgcg aaataagccc gaagagggag aggatccgac
      541 caattggatc cgagggatcg aactgattct gcgaaaattc gaatcaatca atcaaaacga
      601 aggattgaag aaaatcgagg cggaagggca agaattcgat ccgagatatc atcaggcaat
      661 ttcgcacgaa gaagacccga atgttgaatc gggaaaaatt attgaagtat tacaaaacgg
      721 atatatgtta ggagaccgcg tcatcagacc cgctctggta cgcgtagcga aataggagaa
      781 taaaatatgg gaaaaattat aggtattgac ttaggaacta caaactcagt tatggctgtc
      841 atggtcggtg gcgagccgac agtaattccg accgcagagg gagaacgaat ttgcccgtcg
      901 gttgttgctg tcaataagaa tcatgaacga ttggtgggac gtgttgcccg taaccaggca
      961 ataaccaatc cggaaaacac gattttctca atcaaacggt tcatgggacg aaaatacgac
     1021 gatccggaag tccaaaagac attaagccga attccgtaca aagttacaaa agcacccaac
     1081 ggagatgtga gagtggtgtt ggacgggaaa gaatattcgg caccggaaat ttcagccatg
     1141 attttggcaa aaatgaaagc tgacgccgaa gcatatttgg gcgaaaaaat tactcaggcg
     1201 gttattacgg ttcccgctta ttttaatgac agtcagcgga atgcgaccaa agacgccgga
     1261 aaaatcgccg gcttggaagt gctgagaatc attaatgaac cgactgcttc ttcactggct
     1321 tacggcttgg ataagaagac caatgaattg attgcggttt atgacttggg tggcggtaca
     1381 ttcgatatct ccatcctgga tgtgggggac ggtgtattcc aggtacggtc tacatcgggc
     1441 gataccttct tgggcggcga tgacttcgat cagcgtatta tggattatct gatcgatgaa
     1501 ttcaagaaag caaacaacat ggacttgcgc caggatcggc aagcgctgca acggttgaag
     1561 gaagcggctg agaaggcaaa aatcgaactc tcatcgacga tgcagacgga aatcaactta
     1621 ccgtatatta cagccgatgc caccggtccg aaacacctgg tgatgacctt gacacgtgca
     1681 aaggttgagc aattaacttc cgatctgatc gcacggagtt tggaacctgt ccgcaatgca
     1741 ttacgggatg cgaatctgaa gaccagcgat atcaatgaag tcgttttggt tggcggtatg
     1801 acccgtatgc cggcagtcca ggaagcggta aagcgtgaat tcggtaagga accgaacaag
     1861 agcgttaacc cggatgaagt ggtggcggtc ggtgcggcca ttcaagccgg agtcctcggc
     1921 ggagaagtga acgatattct gttattggac gtgacacctc tgaccctgtc ggtcgaaacg
     1981 atgggtggag ttgccacacc gatgatcgag cggaatacta ccattccggc gaaaaagact
     2041 cagatctact cgactgcagc agactcacaa acttccgtcg aaattcacgt cctgcaaggc
     2101 gagcgtccga tggcagccga caataagagc ctcgggaaat tcatcttgga cggaattccg
     2161 ccggctcgac gcggcgttcc gcaaattgaa gtcacctttg atatcgatgc aaacggtatt
     2221 ttgaaagtga cggcggtgga taaggctacc aaccgcagcc agaatattac gatcagcgcg
     2281 tcatccggac tgaccgatgc cgaagtggag cgaatgcgta aggaagccga gatccatgcg
     2341 gaagaagacc gaaaacgcaa ggaaggaatc gagatcaaaa acaatgcgga taacgccgtc
     2401 tatacagccg aaaagttgct ggaggaaaac ggggataaaa tcccggccga tatgaagtcc
     2461 aacatccaat cgaaagccgg tgaattgaag aatatcctca acggaacgga tttcgatacg
     2521 atgaaacgga tgacggatga attgaacaag atgattcaag acatcggaaa catcatgtat
     2581 cagcaacagc aacccggtgc cggacagtct caatcggatc aaggcagcgg tgcatcaggc
     2641 caaaatcctc cgacagggaa cgacgggaat gatgatgttg ttgacggtga attccgcagc
     2701 atgtaatcac ctagttcggt aaaaagaata atcgctgtac cgcgaacctt tataataata
     2761 aggatacggt acagcgattt gttgttccgt catccccggt ttttaccggg aagtttttat
     2821 gatcgagaag tggtaaatta atggtaaaag atcgagatta ttatgaagtc ttgggagtcg
     2881 gcagaaatgc cagctctgat gaattaaaaa cagctttccg aaacctcgca agaaaatatc
     2941 atccggacat taacaaagac cccggggcag aggaaacatt caaagagatc aacgaagctt
     3001 accaggtcct ttccgacccg gataaacgag cagcatacga tcgatatggg aaagccggcg
     3061 tcagcggcgt agggaacgga ttcgattatt ctcaggttga tctctccgat atcctcggtg
     3121 atctcttcgg attcggagga tttggaggct tcggaggctt cggttccaca caatattcat
     3181 cgcgccaacg caatgccccg cgaagggggg cggatctgca gacgaccgtt actttgaaat
     3241 ttgaagaagc atgcttcgga gtcgataaag aagttcaatt ttcccgcgat gaaaagtgtt
     3301 cgcgctgtca cggaacggga gccgaaccgg ggacttcgcc ccaacgatgt tacacctgta
     3361 acggtaccgg tgaaattaaa acaaatcgtc aaaccatgtt cggttctatg gtgcaggttt
     3421 caacctgccc cacctgtaac ggacggggag agatcatcag caccccctgc acgcaatgcc
     3481 acggacgcgg tctcgaacgg aaaaccgtaa aaaaggtggt atctatcccc gccggagtgg
     3541 atacaggcac acgcattcga ttgtcgggag aaggccaacc gggtgataac aacggaccca
     3601 gcggagatct ttacgtggac atccgcgtca accctcataa gtatttccat cggaaagatg
     3661 cggatattca actggatatg aacatcaata tcgctcaagc gactctcggt gatgagatcg
     3721 atgttcctac tatcgacggc acggaaaaac tgaaaattcc cgcaggaacc caacccggga
     3781 gagtatttac cttaaaagga aaaggaattc ctcatttgcg gggcggcgga agaggagatc
     3841 aaaaagtggt cattaacgtt gaaataccga attctctgac acccgaacaa cgggatttga
     3901 tggaacaatt agccggaacc atggggacgg aagtcattcc tcaggaaaaa agtttttggg
     3961 atcgtctcaa ggacgcgtta aatggctgat cccgcctggc tcgaaatcag tgttgtcgta
     4021 gacagtgaga cggcggaagc cgttgccgaa gtgttatccc gctacgccga aaacggcgtt
     4081 gtcgtggaac agggcgtaga ttataacgat gcggaagatg tgggaacgcc gttcggtcct
     4141 tgtcgggtaa ccggttacct tccggtggat gaaacgacgg aagaaaaacg gcagcggatt
     4201 ctggaaggac tttggtattt agggcgcatc accgccgttc cggagccgga atttaagttt
     4261 attgaagacg aagactggat ggcagcatgg aaaaaattct accatccgat tctgatcggc
     4321 gaaaagcttt tgatccttcc cgcttggatg ccatccattg agcccgaacg catcgcgata
     4381 aagattgatc ccagcatggc tttcggaacc ggcactcatc ccactactca gttatgcctg
     4441 gcgatggtag aaaagtacat cgttcccaat gaggacatca tcgatatggg gtgcggttca
     4501 ggaatactct ccatcggagc gttactactc ggcgcccgtc acgctttggg agtggatatc
     4561 gataatgagt ccatgcaaag cagtcgtgag aacgccgaga ataacggagt ggcggacagg
     4621 ctggcgaagg aaatacccgg cgcataccgt ccaa
//
LOCUS       DAOMIV010000101         4652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_18901_length_4652_cov_3.642811, whole genome shotgun sequence.
ACCESSION   DAOMIV010000101 DAOMIV010000000
VERSION     DAOMIV010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18901_length_4652_cov_3.642811"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="PKV59_03515"
     CDS             complement(<1..747)
                     /locus_tag="PKV59_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45851.1"
                     /translation="MDEKGSSELSRVLSDVTLFSAVGGGIRLRNYQRNVAEAVVESVI
                     KQQGKSFVVMFPRQSGKNELQAQIETYLLLVFSREGGEIVKISPTRKPQSINAMRRLE
                     RTLRRNIFTKDLWKKESGYSYRVGNAAIFFLSGAPESNIVGATASLLLELDEAQDILP
                     SKYDHEIAPMAASQNATRVFWGTAWTEDSLLSREMQSAGKAERIDPADRRVFRIGADL
                     VTKEVPAYGKFVAEQIRLLGRDHPLVRTQYF"
     gene            complement(852..1115)
                     /locus_tag="PKV59_03520"
     CDS             complement(852..1115)
                     /locus_tag="PKV59_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45852.1"
                     /translation="MAAGKQKFTLEDLIMMLGDQMNAVYRMADEALPLAEQLKLLDML
                     GKTCSRLASLIEQQRKTQGDDSSRLNTVLQEVLSDLRKQGDHE"
     gene            complement(1329..1637)
                     /locus_tag="PKV59_03525"
     CDS             complement(1329..1637)
                     /locus_tag="PKV59_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQP45853.1"
                     /translation="MPDFGQPFSGLANERKLSEQELIRSIRFMVAAEYEAIQLYMQLA
                     ESTDNKLAIDVLKDIADEERVHAGEFLRLLKELAPDEEDFYQDGAQEVEEMIEKLRKK
                     "
     gene            complement(1743..3371)
                     /locus_tag="PKV59_03530"
     CDS             complement(1743..3371)
                     /locus_tag="PKV59_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQP45854.1"
                     /translation="MRTAIYNGKIYLDRGEFAEAVLIEGDTVLKVGTTAEILASPDLS
                     EKIDCEGRTVIPGLNDSHMHLMMIGEALEQLSLENCKSVAELITASKAYLAQHPEAAK
                     KGLYGRAWNQDYFIDEKRIPNRHDLDQISTEIPVVLERICGHILVTNTKAIEMLGLKA
                     GSPQYKGGVFHLEEDGTPNGVFTEDACRYPLGLIQESTLDEKRMLLLKAMEYAVSRGI
                     TSVQSNDLSYYIKDHNGFIRMIHSLYEEGVAPLRYRLQACFQSPEAFAESFENGLYSH
                     ASDLSDDYFAIGPLKLFKDGSLGARTAMVRHPYLDDPENPDNRGVETTDAATMDRYCA
                     IAAKHGVQVVTHVIGDRAVGDTLDSYEKVLIDGENKYRNSLIHCQLTTLEDLKRIARM
                     GILVQYQPIFLNYDHQICEARVGKELASTSYAFRTLQDLGGKISYGTDSPVEDCNPFE
                     NIYCAVTRKGLDGQPPEGFYPQECVDVYTAVDAYTIGSAYCEFAEDRKGRIRAGYLAD
                     MVILDKDIFEIPVDEIKNVRPVRTIVGGKTVYRS"
     gene            complement(3392..>4652)
                     /locus_tag="PKV59_03535"
     CDS             complement(3392..>4652)
                     /locus_tag="PKV59_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQP45855.1"
                     /translation="MRTGMSQGLSLLAWVLGGLISLLGGLCFAELGASIPRAGGMTVY
                     LNEAFHPLIGFLNAFSGLLIGSPGSIAAIAIALPTAMRTFIPISDSGIKLIAILLIVG
                     LTVFNYFGIRPGSRLQSASMIAKLIPIGIILFASLFLGKVSPDLSMVPEAGPVGFGKL
                     VSMVAFAVIATLWAYEGWTNLNNVTEEVKNPRRNLPLALIIAIGGITVLYTLFNYGIY
                     RVLPFDQIKAFIESGNYYLGTEAAKATLGNAGAVVVTVAMVLSMFGSLNGCILAFPRT
                     YYAMAEEGHFFHAFAELHPKYKVPATGLIVQCIISVILVLVRNLDQLTSLVIFTSVLY
                     NMLTVLAVIRLRKKYPEIPRPFKIWGYPFTVILTALIFLGLVINTFIEDPVSAITGMV
                     IPVIGTVVYFLFDRYRKKEAAAAAQKN"
BASE COUNT         1242 a         1240 c          935 g         1235 t
ORIGIN      
        1 aaaatattgg gtgcggacca acggatgatc tcttcccaaa agacgaattt gttcggcgac
       61 aaattttccg tatgccggca cctctttcgt caccagatcc gctccgattc ggaaaacacg
      121 ccgatcagcc gggtcgatcc gctccgcttt tccggctgat tgcatttccc gtgaaagaag
      181 cgaatcctcc gtccatgcgg ttccccagaa tacccttgtg gcgttttgag aggctgccat
      241 cggtgcgatt tcatggtcat atttggatgg gagaatatcc tgagcttcat ccaattcaag
      301 caacagagaa gcggtcgcac ccacaatatt gctctcgggt gctccgctca ggaagaatat
      361 cgcggcattt cctacccggt aactatagcc cgattctttc ttccataagt ctttcgtgaa
      421 gatgttccgc cgtaacgtgc gttcgagcct gcgcatggca ttaatacttt gcggcttccg
      481 ggtcgggctg attttaacga tttctccgcc ctcccgagaa aaaaccaata ataaatatgt
      541 ttctatctgt gcctgcagct catttttgcc gctctggcgc gggaacatca ccacgaaaga
      601 cttcccctgt tgcttgataa cagactccac gaccgcttcc gccacattcc gctgataatt
      661 tcttaagcga ataccgcccc cgaccgcact gaacaaagtg acatcggaca gcactcggct
      721 taattcactc gatcccttct catccatgtt cgatattatt ttccgatttt cgggaagaaa
      781 cgatcctcgc agccgttcga ccggtatccg ctttcgactt ttggttcagc ggtttcgaaa
      841 cagacaatcc tttattcatg atctccctgc ttccttaagt cgctcagcac ctcctgtaaa
      901 accgtattca aacggcttga atcatcaccc tgcgttttcc gttgttgttc aatcaagctc
      961 gccaatcggg aacaggtttt tccgagcata tccaacagtt tcaactgttc tgccagcggc
     1021 aacgcttcat ctgccatccg gtaaaccgca ttcatctgat ctcccaacat catgatcaaa
     1081 tcttcgagag taaacttttg tttcccggct gccatttttg aatccttttc tcatcgctat
     1141 attagaactt taattctatt tctgaggata acggaatccg aagacacccg caactaccaa
     1201 aaagcggagg ataaaggaga ctgtccgaat aactcggaca atcacaatcg gaattttgtt
     1261 ttcatgcatc ctggaaccaa tacataaaaa aagagtgcat cgggtgatgc actcttttat
     1321 actgcaaatt actttttacg caatttctca atcatttctt cgacttcttg cgctccgtcc
     1381 tgataaaaat cttcttcatc gggcgccaat tctttcaaca gtcgcagaaa ttcaccggcg
     1441 tgtactcttt cttcatccgc aatatctttc aaaacatcaa tggcaagttt gttatcggtc
     1501 gactcggcga gctgcatata caactggatt gcttcatatt cggcggcgac catgaaacga
     1561 attgaacgga ttaattcctg ttcactgagc tttcgttcgt ttgcaagccc ggaaaacggt
     1621 tgtccaaaat ctggcatttt cttttcctcc tctttcttaa acgaatttta caaaacctgt
     1681 caaaaattat aatccgcctt ttcaatcgcc caaataaatc ataacggctt gcctcggaat
     1741 aatcaggatc ggtaaaccgt ctttcccccc acaatggtcc gcacgggacg gacattctta
     1801 atttcgtcga ccgggatttc aaaaatatcc ttatcaagga tcaccatatc cgccaaataa
     1861 ccggctcgga tccgtccttt acgatcctca gcaaattcac agtaggcgct gcctatggtg
     1921 taagcatcga cagccgtata gacatccaca cattcttgcg gataaaatcc ttccggaggt
     1981 tgcccatcca gccctttacg agttacggca caatagatat tttcaaacgg attacaatct
     2041 tctaccggcg aatccgttcc gtaggagatt ttccctccca aatcctgtag tgtccgaaac
     2101 gcataggaag tcgaagccag ttccttcccg acacgcgctt cacagatttg atgatcataa
     2161 ttcaaaaaaa tcggttgata ttggactaag atccccattc ttgcgatacg cttcaaatct
     2221 tccagcgtgg tcagttggca atgaatcagg ctgttccgat atttgttttc accgtcgatc
     2281 agaacttttt cgtaactgtc gagcgtatca ccgaccgcgc gatcaccgat gacgtgcgta
     2341 accacctgga ctccatgctt tgcggcaatt gcgcaatatc gatccattgt tgccgcatca
     2401 gtcgtctcaa cacccctgtt atcgggattt tccgggtcat ccaaataagg atgccgtacc
     2461 atcgcggtgc gggcacctaa actgccgtcc ttgaacagct tcaacggtcc gatggcaaaa
     2521 taatcatcac tgaggtcgga agcatgagaa tacagcccgt tttcaaaact ttccgcgaaa
     2581 gcttccggcg attgaaagca tgcctgcaaa cgataacgaa gcggagcaac tccctcttca
     2641 tacagactgt gaatcattcg gataaatccg ttatggtctt taatataata actgagatcg
     2701 ttcgattgaa cgctggtgat tccgcgggaa acggcatatt ccattgcctt cagcaacaac
     2761 attcttttct catcaagagt cgattcctga attaacccca gaggatagcg gcaggcatct
     2821 tccgtaaaaa cgccgttagg agttccatct tcttcaagat gaaacacacc tcccttatat
     2881 tgaggagatc cggctttcaa acccaacatt tcgattgctt tggtatttgt aactaaaata
     2941 tgcccgcata tccgttctaa taccaccggt atttcggtgg aaatttgatc caggtcgtga
     3001 cggttgggaa ttcgcttctc atcgataaaa taatcctgat tccaagctcg tccgtatagc
     3061 cctttctttg cggcttccgg atgttgagcc agataggctt tggaagcggt gattaattcc
     3121 gcgaccgatt tgcagttctc caaggataat tgctctaacg cttctccgat catcatcaga
     3181 tgcatatgag aatcgttcag gcccggaata accgtccggc cttcgcaatc gattttttcc
     3241 gaaaggtccg gcgaagctaa aatttcggct gtcgtcccga ccttcaaaac ggtatcccct
     3301 tcaatcaata ccgcttcggc gaattccccg cggtctaaat agatttttcc gttataaatt
     3361 gctgtacgca ttttttcctc cgtttaacct tttaattctt ctgtgcggcc gcggcagctt
     3421 ctttctttcg atatcgatca aaaaggaagt aaacgaccgt accgattacc ggaatgacca
     3481 tccccgtaat cgcacttacc ggatcttcga taaaagtatt gattaccaaa ccgaggaata
     3541 tcaatgccgt caggattacc gtaaacggat agccccagat tttaaaagga cggggaattt
     3601 ccggatattt tttccgcaac cgaataacag ccagtaccgt cagcatatta tagagaacgc
     3661 tggtaaatat caccaagctg gtcagctgat ccaaatttct taccaaaacc aaaatcaccg
     3721 aaataatgca ttgaacaatc aaccccgttg ccgggacctt atatttcgga tgaagttcgg
     3781 caaaagcatg gaaaaagtgt ccttcctccg ccatcgcata ataagtccgg ggaaaagcca
     3841 aaatacagcc gttcagcgat ccgaacatgg acaaaaccat tgcgacggtc accaccaccg
     3901 ctccggcgtt tcccagcgtt gctttagccg cttccgtacc gagataataa ttccccgatt
     3961 cgataaaagc cttgatctga tcaaacggca acacccgata gattccgtaa ttaaataagg
     4021 tatataaaac cgtaattccg ccgattgcga tgattaaagc cagaggcaaa ttccggcgcg
     4081 ggttctttac ttcttccgtg acattgttga gatttgtcca accttcataa gcccacagcg
     4141 tcgcaatcac ggcaaatgcg accatgctga caagcttacc gaaaccgacc ggtcccgctt
     4201 cgggaaccat gcttaaatcg ggagaaactt ttcccaagaa taatgaggca aagagaatta
     4261 tgccgatcgg aattagtttt gcgatcatgg aagcgctttg caatcggctt cccggtcgaa
     4321 ttccgaaata attaaaaacg gttaatccga caatcagaag aatcgcaatg agcttgatac
     4381 ccgaatcact gatcggtata aaagttcgca tggcggtggg taacgcaatg gcaatcgccg
     4441 caatcgagcc cggacttcca atcagtaaac cactgaaggc gttcaaaaaa ccgataaggg
     4501 gatgaaatgc ctcatttaga tagacagtca taccgcccgc acggggaatc gaagcgccta
     4561 gttcggcaaa acacaatccc cccaataaag agatcaatcc tcccaatacc catgccagca
     4621 gcgagagtcc ctgactcatc ccggtccgca tt
//
LOCUS       DAOMIV010000102         4644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_18953_length_4644_cov_5.128568, whole genome shotgun sequence.
ACCESSION   DAOMIV010000102 DAOMIV010000000
VERSION     DAOMIV010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18953_length_4644_cov_5.128568"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PKV59_03540"
     CDS             complement(<1..1194)
                     /locus_tag="PKV59_03540"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQP45856.1"
                     /translation="MKPEVKRYTAEVGGKTITFETGKLAAQAGGAVTIQLGESVVFSA
                     ATMGDEVHEEQDFFPLTVEYEERMYAGGRIPGSFFRREGRPAGDTILIGRLVDRPIRP
                     LFSQDIRNEVQVILYSLSADFENQLDILAINAASASLAISDIPWDGPVGAVRVGLING
                     EYVINPTFQQMEDSDLDLRIAGTADAILMVEAGSNEVSNEAMVGAIEAGHRAIQPLIE
                     AQLKMAAEVGKPKREVKRSCIPQTTVDTVFEKYNQSMETLLDAPHDKHELADRLAELK
                     ADAVEFFAGEDSEKQSEYGNAFEEAYRKVIRSRILDRHLRPDGRALNEIRPIWCEVDV
                     YPRAHGTGLFTRGETQVLTSATLGTPRDAQEIDSLSPVENKRYIHHYNFPPFCTGEVK
                     RLGGQS"
     gene            complement(1408..1680)
                     /gene="rpsO"
                     /locus_tag="PKV59_03545"
     CDS             complement(1408..1680)
                     /gene="rpsO"
                     /locus_tag="PKV59_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQP45857.1"
                     /translation="MALTKEEKAEIFKNFGLHEGDTGSVEVQIAMLTKRIADLTNHLK
                     TSKNDESSRRGLLKMVGQRRRLLAYLRKNDYASYVALNEKLGLRVK"
     gene            complement(1810..2625)
                     /locus_tag="PKV59_03550"
     CDS             complement(1810..2625)
                     /locus_tag="PKV59_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45858.1"
                     /translation="MPAVNLLNLRKEITSLLWEFTDPESFRDKLAQLLDHYRIMSFRS
                     GSGVLTSYQTPELHVPQLVLHELELALYQTIREQPQAALTLCDSLWNDVNLEMHTIAT
                     RMLGQIPIEYMDQVLTRINEWTQKGLKPSELKVFFRNAAGLIRQKQPEKLMRSAQDWC
                     AEPDNKNRERGIQLLIYLVEDPAFINLPAVYTALEKMLPDADAVLQPDLTELIGLLLS
                     RSEIETSFFVRQMLENPDSGKIIRRIARKLLPKIPESSRESIQNLLRTSQKES"
     gene            complement(2659..4095)
                     /gene="proS"
                     /locus_tag="PKV59_03555"
     CDS             complement(2659..4095)
                     /gene="proS"
                     /locus_tag="PKV59_03555"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQP45859.1"
                     /translation="MAEDKLTSRSENYSDWYNQVVLRADLADYAPVRGCMVVKPYGWA
                     LWENIQRELDRRFKETGHVNAAFPLLIPKSFFEKEKEHVEGFAPELAVVTIGGGEELE
                     EPLIIRPTSETIIGYMYSKWIKSYRDLPVLINQWANVVRWEMRTRLFLRTLEFYWQEG
                     HTAHATAEEAQEETLRMLDVYADFAINEAAVPVIKGRKSETEKFAGAINTYSIEAMMG
                     DTKALQAGTSHYLGQNFAKAFDIKYLDQNNTLQYCWTTSWGLSTRFIGAIIMTHGDDQ
                     GLILPPRLAPYQIVIVPIYKNDQERSVVMEYIEKVQSQLKGFRYRLDDRSELTPGFKF
                     NEWELKGVPLRLEIGPKDVEKHAIMAARRDIPGKAGKISLSIENLDLKIENLLNEIQQ
                     SLLEKATEFRDQNIHFADSYDELKQIVENGWAEAWWCGSAECEAKIKEETKATTRCMP
                     LDEKIRGKGKCVYCGAEAEKRIYFSKAY"
     gene            complement(4139..4360)
                     /locus_tag="PKV59_03560"
     CDS             complement(4139..4360)
                     /locus_tag="PKV59_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45860.1"
                     /translation="MNSEEIDFSRSEIEIFWDGILSRERATILSTFLSVTLAEQESCL
                     KHLNRMAHEEGWHEEQRKSAQIALDVLNR"
BASE COUNT         1187 a         1141 c          939 g         1377 t
ORIGIN      
        1 actctgcccg cccagcctct tcacttcacc ggtgcagaac ggcggaaagt tgtagtgatg
       61 aatatatcgt ttattttcca caggtgagag tgaatcaatt tcctgtgcat ctcgcggtgt
      121 acccagcgtg gcgctggtca acacctgagt ttctccgcgg gtaaataagc cggtaccgtg
      181 agctcgcgga taaacatcca cttcacacca aatcggacga atttcattca atgcacgccc
      241 gtccggtcga agatgtcgat ccaagatacg gcttcggatg actttgcggt aagcttcttc
      301 gaaagcattt ccatattctg actgcttttc cgaatcttcg ccggcaaaaa attcaaccgc
      361 atcggctttc aactcggcaa gccgatcagc caattcatgt ttgtcatgtg gcgcatccag
      421 caaagtctcc atcgattggt tatatttttc aaaaaccgta tcgaccgtag tctgcggaat
      481 gcaggagcgt ttcacttcgc gcttgggttt gcccacttcc gcagccattt tcaattgagc
      541 ttcgatcaga ggttggattg cccgatgacc ggcttcgatc gctccgacca tcgcctcgtt
      601 gctgacttca ttcgacccgg cttctaccat caaaatggca tctgccgtgc cggcaattcg
      661 gagatccaaa tccgaatctt ccatttgctg aaaagtcggg ttaatcacat attcgccgtt
      721 gatcaatccg acacggacgg caccgaccgg tccgtcccat gggatatccg agatagccag
      781 agatgccgaa gcggcattga tcgccaaaat atccagttga ttttcaaaat cggcggagag
      841 gctatagagg ataacctgaa cttcgtttcg aatatcttgt gaaaacaaag gccggatcgg
      901 acgatcgacc aatctgccga tcaggattgt atcaccggcg ggacgtcctt cacgccggaa
      961 aaaagaaccc gggatgcgac ctccggcata cattcgttct tcatactcta cggtcaatgg
     1021 gaagaaatcc tgttcttcat gaacttcgtc ccccatcgta gctgcggaaa atacaaccga
     1081 ttcacctaat tgaatcgtta cggcaccacc tgcctgagcc gctaattttc cggtctcaaa
     1141 cgtgatggtt ttaccgccca cttcggcggt atatcttttt acttctggtt tcattcttgt
     1201 tccatttctt tcaccaggcc agggactgaa aaaaaacaag aaaaacatct gctttcccat
     1261 ttttttttgg tttcagcaga tgatttgatt ctgatctttt ttcaatgcct aaccatggtg
     1321 aatactgata aataataaag gtttatacct cttttttaaa aaagagcaga tgatttcaat
     1381 ccacctgctc tctgactcgc tgattcgtta tttaaccctc aggcctaatt tttcatttaa
     1441 ggccacataa cttgcataat cgttttttcg caggtaagct aacaaccggc gacgctgacc
     1501 gaccattttc agcaatccac gacgtgagct ctcatcgttt ttgcttgttt taagatgatt
     1561 cgtcaaatcg gcgattcttt tggtaagcat ggcaatctgc acttccacag atcctgtatc
     1621 accttcatgc aatccaaaat ttttaaatat ttctgctttt tcttccttcg ttaaagccat
     1681 gacaatgctc ctctgattaa ttttttgcat gtctccaaaa attcaagcat ttacctgaat
     1741 tcggacaagt ctttacaaaa ttctaccatt aattatcgat ctttttataa atcgatgatc
     1801 ccggtaaact caactctctt tctgcgaagt ccttaaaaga ttttggatcg attcccggga
     1861 agattctgga attttcggaa gtaactttcg tgcgatccgg cgaataatct ttccgctgtc
     1921 cggattttcc aacatttggc gaacaaaaaa agaggtttca atttcggatc ggctcaataa
     1981 caaaccgatc aattcggtta aatcgggttg taagacggcg tcggcatccg gaagcatctt
     2041 ttccagcgcg gtatagactg ccggaagatt aatgaatgcc ggatcttcaa ccaaataaat
     2101 caataattgg atcccgcgtt cccggttttt attgtcgggt tccgcacacc agtcttgagc
     2161 tgatctcatc agtttttccg gttgcttttg tcggatcaga ccggctgcat tccgaaaaaa
     2221 gactttcagt tcggaaggct ttaatccttt ttgggtccat tcatttatac gagttaaaac
     2281 ctgatccata tattcaatcg gaatttgccc caacattcgg gtcgcaatgg tatgcatctc
     2341 caggttcaca tcattccata gcgaatcgca aagagtcagt gcagcctgag gttgttcccg
     2401 aattgtctga taaagcgcta attccaattc atggagaacg agttgaggaa catgcagttc
     2461 cggtgtctga taagaagtta agacaccgga gcccgatcga aaagacataa tccgataatg
     2521 atcgagcaat tgcgccaatt tatcccggaa tgattccgga tcagtaaatt cccaaagcag
     2581 ggaagttatt tccttacgta aatttaacag atttacggcg ggcatatcgg ggttccctca
     2641 attgcatgtg atgttctatc aatatgcttt ggaaaagtaa attcttttct ccgcttccgc
     2701 accgcaatag acacattttc cttttccccg tattttttca tccagcggca tgcagcgggt
     2761 ggtggctttt gtctcttctt ttattttggc ttcgcattcc gccgagccgc accaccaagc
     2821 ttccgcccag ccattctcaa cgatttgttt cagctcatca tatgaatcgg caaaatgaat
     2881 attttgatct ctgaattccg tagccttttc aagcaaagac tgttgaattt cattcagcag
     2941 attttctatt ttcagatcca gattctcgat tgagagagag atctttcccg ctttccccgg
     3001 gatatcccgt ctggctgcca taatcgcatg tttctccaca tctttcggcc cgatttccaa
     3061 gcgcagaggg acacctttta attcccattc gttgaattta aatccgggag ttaattcgct
     3121 tcgatcatcc aaccggtatc gaaaaccttt tagttgggat tgcacttttt cgatatattc
     3181 catcacaacc gaacgttctt ggtcattttt gtaaataggc acgataacga tctggtaagg
     3241 agccaatctg ggaggcaaaa tcaagccttg atcatcgccg tgagtcatga taatcgcgcc
     3301 gatgaagcgg gttgaaagtc cccacgaggt cgtccaacaa tattgcaacg tattattttg
     3361 gtccaggtat ttgatatcaa atgccttggc aaaattttgg cccaaataat ggctggtacc
     3421 tgcctgtaag gcttttgtat cccccatcat ggcttcgatg gaataagtat tgattgcgcc
     3481 cgcaaatttt tcggtttcgc tttttctgcc tttgataacc ggtacggcag cttcattgat
     3541 ggcgaaatca gcataaacat ccagcatccg taatgtttct tcctgcgctt cttccgcggt
     3601 cgcatgagcg gtatgtcctt cctgccaata aaactccagt gttcgcaaaa acaaacgggt
     3661 gcgcatctcc caacggacga cgtttgccca ttgattaatc aagaccggca gatcacgata
     3721 gctcttgatc cattttgaat acatatagcc gataattgtt tcggaagtcg gacggataat
     3781 taacggttct tccaactctt cccctccgcc gattgtgaca accgccagtt ccggtgcgaa
     3841 gccctccacg tgttcttttt ctttttcaaa aaaagacttg ggaattaata acgggaaagc
     3901 ggcattcaca tggccggtct ccttaaagcg tcgatccagt tcacgctgaa tattttccca
     3961 tagcgcccat ccgtacggct tgacgaccat gcaaccgcgg accggtgcat aatccgccaa
     4021 atcagcccgt aaaacgacct ggttatacca atcggaataa ttttcgctcc gtgaagtaag
     4081 tttatcctct gccatttttc ttttccgttt tccttcgttc gaatttcagc ccattatact
     4141 atcggttcaa tacatccaaa gcgatctgtg cagatttacg ctgttcctcg tgccatcctt
     4201 cttcatgcgc catacggttt aaatgtttca gacaactttc ctgttcggca agtgtaacgg
     4261 acaaaaatgt tgaaagaatg gttgcccttt ctcttgacaa tattccgtcc caaaaaattt
     4321 ctatctccga ccggctaaaa tcgatttcct ccgaattcat cgctgttctc ctttcttgaa
     4381 tgaatccatt ttatcatttc accgaattat ccaaaggtca atgcaagtgg taccacctta
     4441 agaacaattc cgatgaagtg atcgttttct gctaaaaatt tacacctatt ttcttagaat
     4501 agcgtataaa tagaaaggag ggattatata taaatgactg ctacaatcga tgattacgga
     4561 ttggtttttt cgggtggagg cacacgcggt gcctacgaaa tcggtgcatg gaaggcactg
     4621 aatgagttgg gaataaaaat acag
//
LOCUS       DAOMIV010000103         4636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_18990_length_4636_cov_3.870989, whole genome shotgun sequence.
ACCESSION   DAOMIV010000103 DAOMIV010000000
VERSION     DAOMIV010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18990_length_4636_cov_3.870989"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKV59_03565"
     CDS             complement(<1..392)
                     /locus_tag="PKV59_03565"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQP45861.1"
                     /translation="MEFYSYGFVIGPFLIHYYSLCFLAGVFSATLLASRTGKKRGIAP
                     DTIWSCLFWLLIAGIIGARLWHVFLPSKSSGLTLSYYLHNPVKILRFGTAGWEFPERF
                     SEDISGYGCFAGNMDSKSANSPTQSLPG"
     gene            complement(420..1058)
                     /locus_tag="PKV59_03570"
     CDS             complement(420..1058)
                     /locus_tag="PKV59_03570"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQP45862.1"
                     /translation="MNENISNNSSNQPEKPKTFSDQCRIWFRGFGETCSRFLLKIGLT
                     ANTVTIIGCAGHIIAAYLAAMGHFTWAGILLVFFAVTDFFDGTMARLSTGGKGTRFGA
                     VLDSCTDRYAEFFIFGGLIYYYAVHEDYLTMMVAYIAIMGSILVSYTRAKGEIERLDM
                     KLGIMSRLERYLFLVPCLLFNIPFIAMWAIAIGSHITAIQRIQYMRKELKEN"
     gene            complement(1077..3251)
                     /locus_tag="PKV59_03575"
     CDS             complement(1077..3251)
                     /locus_tag="PKV59_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQP45863.1"
                     /translation="MPEKENKQENEPQKPSGSRLKSESLIPDILALGCIVTGLLLWVG
                     FTGKTDGRFILRMRNFFDDWIGTGRIIFVVLFLGLGTGIFLSRYTTVKIRIPLKKIGL
                     IILLFFILLALASNSDHFSGGKIGDTLFGQLTAVIGEIPATILLWGIIAVLFYFLFDL
                     NRPLSESAVFIKKKVAHFCASKPIRSSDEVTLFRRRLMKENIEKEENDPLEKNITNHP
                     IDSVIGGNEIKGKSKPRTNRLPPINLLEPEMGFLNKNIDVQSRIDEIESAMEEFGTPV
                     KVIGYSIGPAIIQYQVVPGALEKNGSHGTVIPKKIRVAQVAQMERDLAVRMGVANLSI
                     QAPVPGENFIGIDIPNPDSLKVRLRPLMESREFQSKTSPLNVALGRDISGKPVVVDLE
                     QMPHLLIAGTTNSGKSILIRSMAVCLVMNNTPETLRLIMIDPKRVELFRFNGLPHLLG
                     KVETEYDRSISVLGWAVQEMKNRYKLFETVGVRKLSLYNQYAVRNGQKPLPYIVIFID
                     ELAEIVKGADKLGQEYIDTLASLARATGMHLVVATQRPDTTVITGKIKTNIPARIALN
                     VASAIDSRVIMGKQGAEKLLGRGDMYFVDPSLNVPIRIQGPLLTDQEIDSVVALWKKL
                     SPKPVEEAEAPWEEMIEDAEKEVNGKEEKDLKAAIRLVCRTRKASASYLQGKMNISFP
                     KASRLLDRMEQIGVVGPIQVGGKAREVLWSEEEADSYGENAI"
     gene            complement(3308..>4636)
                     /locus_tag="PKV59_03580"
     CDS             complement(3308..>4636)
                     /locus_tag="PKV59_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45864.1"
                     /translation="VSALGVLVGTFPLMIYGSGFLLRHELGFWLVNLIANILILLMVL
                     LLGYSVITFCVSWSYRVVRLRLMEWILRGPVTAGLTLGLVTIIRRSSGTLNINVGGWT
                     SLLMVVSIVVLEYLITLLMPLFERNSISGYGNQDYQIVSGLENMMIFSSELETYLESI
                     ISALCDIYQAKGVFVAVTDSMGNVENLIHVGETSLSDPIIFSEKIEALQGFTDEPITD
                     DGSILLPVFYRDLTENKTWLLAIIGITDPAVTGEESEPQSALKVAADKIRIVLWQRWY
                     LGRTFNVLRELSNDEKLGSYRKIGLLNTAEVLDQKSDYELEEVSTWVRDALTHYWGGP
                     RLTENPLIRLDIVQQEMNGDSNNATNALRTILKKAIDQIRPSGERSISGEWTLYNILD
                     LKFIEGLKVKEVAKKLAMSEADLYRKQRVAIEELAKTIIRMEKEHNSSHL"
BASE COUNT         1293 a         1192 c          921 g         1230 t
ORIGIN      
        1 caacccggga gagattgtgt cggtgaattt gctgattttg aatccatatt tcctgcaaaa
       61 caaccataac ccgatatatc ctccgagaat cgctccggga attcccaacc cgccgttcca
      121 aacctaagga ttttgacggg gttgtgtaaa taatagctta gcgtaagtcc cgaggatttg
      181 gaaggcaaaa acacatgcca aagccgggca ccgataatac cggcgatcaa aagccaaaac
      241 agacaactcc aaatcgtatc cggtgcgatt ccgcgctttt ttccggtccg gcttgcgaga
      301 agtgtcgcac tgaagactcc ggctaagaag cataaactat aataatgaat aagaaacggc
      361 ccgatcacaa acccatatga ataaaattcc aatggtcccc gcctgacttt tttgttggtc
      421 taattttctt tcaattcttt ccgcatatat tgaatgcgct ggatcgccgt gatatgagat
      481 ccgatcgcta tcgcccacat ggcaataaac gggatattaa acaacagaca gggaaccaag
      541 aaaagataac gttccagtcg tgacatgatg ccgagcttca tatccaatcg ttcgatttcg
      601 ccttttgccc gggtataaga taccaggatc gaccccataa tggcaatata cgccaccatc
      661 atagtcagat aatcctcatg aaccgcatag taataaatca aaccgccgaa aatgaagaat
      721 tcggcatagc gatcggtaca ggaatccaga accgctccga atcgggtgcc cttcccgccg
      781 gtggaaagcc gagccattgt tccgtcaaag aaatcggtaa ccgcaaaaaa taccaagaga
      841 atacccgccc aggtgaaatg tcccatggca gctaaatatg ccgcgataat atgcccggca
      901 cagccaataa ttgtcacggt atttgcggtc agaccgatct tcagcagaaa tcgagaacag
      961 gtttcgccga aacccctgaa ccatattcga cattgatcgc tgaaggtttt cggtttttca
     1021 ggttggttcg atgaattgtt tgaaatattt tcgttcataa tgtcttctgt ctccttttat
     1081 atggcatttt ctccgtaaga atccgcttct tcttcagacc ataatacttc acgcgctttc
     1141 ccgccgacct gaatcggacc gaccacacca atctgctcca ttcgatccaa cagccggctg
     1201 gctttcggaa aactgatatt catttttccc tgtagatacg aagcgctcgc tttccgtgtc
     1261 cggcaaacca ggcgaatcgc cgctttcaaa tccttttctt ctttaccgtt tacttccttt
     1321 tccgcgtctt cgatcatttc ttcccaagga gcttccgctt cttcgacggg tttcggcgat
     1381 aattttttcc ataaggcaac gaccgaatca atttcctgat cggtcagtaa aggtccctgg
     1441 attcgaatcg gcacattcag actgggatcg acaaaataca tatcccccct gcctaataat
     1501 ttttccgcac cctgtttccc cataatcact ctcgaatcga tcgccgaagc aacatttaat
     1561 gcgattcgcg cgggaatgtt ggtcttgatt ttgccggtaa tcactgttgt atccggtcgt
     1621 tgggttgcga caaccaagtg cattcccgtc gcccgtgcca acgatgccaa tgtatcgata
     1681 tattcctgac ccaatttatc cgcgcctttg acaatttccg ccaactcatc gatgaaaatc
     1741 acgatataag gtaatggttt ctgcccgttt cgaaccgcat attggttata taaagataat
     1801 ttgcgaactc ccaccgtctc aaataattta taacggtttt tcatttcctg aactgcccag
     1861 cccaaaaccg aaatgcttcg gtcatattcg gtttcgactt taccgagcaa atgaggcaat
     1921 ccgttgaaac ggaaaagttc cacccttttg ggatcaatca taatcaatcg caacgtttcc
     1981 ggcgtgttat tcatgaccag acaaaccgcc atcgaacgga tcaaaatgga tttaccggaa
     2041 ttggtggtgc cagcaatcaa cagatggggc atctgttcca aatcaaccac caccggttta
     2101 ccggaaatat ctcttccgag tgcgacattc aacggtgatg tcttggattg aaactcccgg
     2161 ctttccatta aagggcgcag acgaactttc agggagtccg gattggggat atcgatcccg
     2221 ataaaatttt ctcccggcac cggtgcctga atcgaaagat ttgccacgcc cattcgaaca
     2281 gccaaatccc gctccatctg cgcaacctga gcgacccgga ttttcttggg gattaccgtg
     2341 ccgtgacttc cgttcttttc caaagcgccc ggaacaacct gatattggat aatcgcgggt
     2401 ccgatactgt aaccgataac tttcaccgga gtgccgaact cttccatggc ggattcaatc
     2461 tcatcgattc gtgactgaac atcaatattc ttatttaaaa atcccatttc cggttccagc
     2521 aaattaatgg gcggcaaccg gttggttcgc ggcttgcttt ttccctttat ttcatttccg
     2581 ccgataacgg aatcgatcgg atgattcgta atgttttttt ccaatggatc gttttcctct
     2641 ttttctatat tttctttcat taatcgtctg cgaaaaagtg taacctcatc ggaggatcgg
     2701 atcggttttg aagcgcagaa atgtgcaact ttctttttta taaaaaccgc cgattcggac
     2761 aaagggcgat tcaaatcaaa aagaaaataa aataggacgg caatgatacc ccataaaaga
     2821 atcgtcgcgg ggatttcccc gatcaccgcg gtaagctgac cgaacaaagt atccccgatt
     2881 tttcccccag agaaatgatc actgtttgaa gcgagtgcca gcaatatgaa aaacaacaga
     2941 ataattaagc cgattttttt caacggaata cggattttta cggttgtata ccggctgaga
     3001 aaaatgccgg ttccgagccc gagaaaaagc acaacaaata tgattcttcc cgtcccgatc
     3061 caatcatcaa aaaaattccg cattctcaaa ataaaacggc cgtcggtttt tccggtaaag
     3121 cccacccata acagcaagcc agtcacaata cagccgagcg cgaggatatc cggaattaaa
     3181 gattcgcttt tcagccgcga gcccgaaggc ttttggggtt cattctcttg cttgttctct
     3241 ttttcaggca ttctctttat ctgtcaacgg ataaatgaat caaattttat atcaaaccaa
     3301 aaacacctta caggtgggaa gaattatgtt ccttttccat acgaataatc gttttcgcca
     3361 gttcttcgat tgcgacccgt tgctttcgat atagatcagc ttcggacatc gccaactttt
     3421 tcgccacttc tttcaccttt aaaccttcga tgaatttcag atccaaaata ttatacagag
     3481 tccactctcc cgaaatagac cgttcgccac tcggacggat ctgatcaatc gcttttttca
     3541 aaattgtacg caaagcattt gtcgcattat tgctgtcacc gttcatttcc tgttgaacga
     3601 tatccaatcg gattaaggga ttttcggtca accgtggtcc gccccaataa tgggttaacg
     3661 catctcgaac ccacgtgctg acttcttcca gctcgtagtc ggatttttga tctaatactt
     3721 ccgcggtgtt taataaaccg atcttacgat acgaacctaa cttttcatcg ttggagagtt
     3781 ctcgtaaaac attgaatgtt cgtcccaaat accaacgttg ccataaaaca attcgaattt
     3841 tatccgccgc aacttttaaa gccgattgcg gttccgattc ctcgccggtg acagccgggt
     3901 cagtaatacc gataatcgcc aacaaccagg ttttattttc ggtaagatca cgatagaaga
     3961 ccggcaacaa aatgctgccg tcatccgtaa tcggttcgtc ggtaaatccc tgaagcgctt
     4021 cgattttttc ggaaaaaata atcggatcag atagactcgt ttcaccgaca tgaatcagat
     4081 tctcaacatt gcccatggaa tccgttacgg caacaaagac ccctttcgcc tggtatatat
     4141 cgcatagggc gctgataatc gactccaaat aggtttccag ctccgaggaa aatatcatca
     4201 tattttcgag accggaaaca atttgatagt cctgatttcc gtagccggaa atgctgtttc
     4261 tttcaaacaa cggcatcaat aaggttatca gatattccag cactacgatc gaaacgacca
     4321 tcaaaaggga cgtccatccg cctacattga tattcaacgt tcccgagctg cgccggatga
     4381 tcgttacgag tcccaaagtc agcccggccg tcaccggtcc acgtaaaatc cattccatca
     4441 ggcgcaagcg aaccacccga taggaccagg aaacacaaaa agtgattacg gaataaccca
     4501 acaaaagcac catcaacagg atcagaatgt tggcgattaa attgaccagc caaaatccaa
     4561 gttcatgccg cagcagaaag ccggatccgt aaatcatcaa aggaaatgtc ccgaccaaaa
     4621 ctcctaacgc gctgac
//
LOCUS       DAOMIV010000104         4605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_19183_length_4605_cov_3.848132, whole genome shotgun sequence.
ACCESSION   DAOMIV010000104 DAOMIV010000000
VERSION     DAOMIV010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19183_length_4605_cov_3.848132"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="PKV59_03585"
     CDS             <1..689
                     /locus_tag="PKV59_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45865.1"
                     /translation="GQHPPRLIPEMIQLAENGYDIVQTQRLDDEEKSSFKKRTSKWFY
                     QLINQIGDTKTLPGGADFRLITKQVLDNLLAMPEYHRFLRGMVSWLGFRSVVIPFHPE
                     ERLAGESKYSLKKMLKLGSDAVFSFSLVPLYVGLSAGGIFFILAIIEVIYVLSFWISG
                     RSAELTPGWSSLMFMMLIIAAVLMILLGFIGIYVGYIFQEVKHRPVFVIKEKKVHPNN
                     QKEQSEEENL"
     gene            686..1699
                     /gene="galE"
                     /locus_tag="PKV59_03590"
     CDS             686..1699
                     /gene="galE"
                     /locus_tag="PKV59_03590"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQP45866.1"
                     /translation="MKFLVTGGAGYIGSTVVSALEDAGHQAVILDSLVTGRIEFTRGK
                     IFYLGDIADRKILTQIFADHPDIFAVLHCAALIVVPESVEKPYEYYRENVAKSNEMFH
                     ILYTLGCQRIVFSSSAAIYDKAENFMVTEDSPLAPNSPYSRTKYMMEMILQDTSNAYN
                     MRGIALRYFNPIGADPKMRSGMHVKEPSHVLGKLVDTALGKQDAFTVTGTNWPTRDGS
                     GIRDYIHVWDLAQAHVKALAEFDQVFERTGDPNINFIPINLGSGKGVTVFELVNAFER
                     VYGKKINIRTAPPRPGDVAGAYANADRAKKLLGWETQLSIEDGIRTALEWGKIRESIL
                     HWD"
     gene            1831..2367
                     /locus_tag="PKV59_03595"
     CDS             1831..2367
                     /locus_tag="PKV59_03595"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQP45867.1"
                     /translation="MQPSLTHGLNILYTGNGKGKTTAAMGLLFRAWGNNMSVAGIQFI
                     KSPEIVKGEMIACRRLGIPFETLGKGFVFEKFDTTIHAEAARSAWDFTREVILHRNLD
                     MLLLDEITYLFVYKWLDCNEFIQWIRENKPPTMHLILTGRNAPQELIEFADLVTEMKE
                     IKHPYTTQRIPAQIGIEY"
     gene            2415..3311
                     /locus_tag="PKV59_03600"
     CDS             2415..3311
                     /locus_tag="PKV59_03600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HQP45868.1"
                     /translation="MNLSKKRTIIFIIITFVITWAAEFMLWKGGGLKNPRVQIYLSII
                     MMIPMIGSFLTRWITKEGLENLWLRLDFKANIKTYLIAWFGPALLILFGSALYYLIFP
                     AKFDPEMSVLRQSLNALGEQQTAELTTQKLLLSILSQVLLALIMSPILNAIPCLGEEI
                     GWRAYLLPKLADFLPRKKAVVISGVVWGIWHAPIIAMGYNYGENYPLFPWLGIIGMIL
                     FCIFVGSFLAWLTFRMESALPAAIAHGAVNGMASVGIFFLRGTPEPFLGPYPVGIVGG
                     FGFIVIGILCYLNIEKAKPLQS"
     gene            3463..4437
                     /locus_tag="PKV59_03605"
     CDS             3463..4437
                     /locus_tag="PKV59_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45869.1"
                     /translation="MMKNKKQLFVLMILVAMFAIGIATVQAEGIEPYPSYTITSVTVD
                     QSVSILTLNFPADTEFIVRMKDLNSVSDYIDVAKFNTQSGGSFTATFPIPAELVGKKS
                     IGMLLSNNNGIEIPSTFSNEAMTETVATVCDYSVLPSFTIDSVVKGKSITVTTGTFPA
                     NQSFTVKMGYYAAGAPAAHHHCGGHDCPTSNPWDPWMDVDGTPEPEMYPIPDPSFSWM
                     FGPMIPGCGTSGCVNFHLGGATPHAGLIPTYFVGFEVGTYDSADGSPQTVSYDIPEEL
                     KGYSPIIVRFEDKGSCGFYGYNYFWNADFPVTSSESVPEVQIETVSQQ"
BASE COUNT         1239 a          978 c         1085 g         1303 t
ORIGIN      
        1 acggacaaca cccgccgcgc ctcattccgg aaatgattca attagcggaa aacggctatg
       61 atatcgttca gactcaacga ttggatgatg aagaaaagag ctcctttaaa aaaagaactt
      121 ccaaatggtt ctatcagttg ataaatcaaa tcggtgatac caaaacgtta cccggaggag
      181 ccgattttcg tttgatcaca aaacaggtat tggataattt actggcgatg ccggaatatc
      241 atcgtttttt gcgcggcatg gtgtcatggt tgggttttcg atcggtcgtc ataccttttc
      301 accccgaaga aagattggcg ggagaatcaa aatattctct gaaaaaaatg ctcaagctcg
      361 gttcggatgc ggtcttttct ttttcacttg tcccccttta tgtcggactt tcagccggcg
      421 gcatattctt cattctggcg atcatcgaag tcatttatgt cctgtcattc tggatttcgg
      481 gtagatcggc ggagttaact cccggctgga gctcattgat gtttatgatg ctgattatcg
      541 ccgcggtttt gatgattttg ctcggattta tcggaattta tgtcggatat attttccagg
      601 aagtaaaaca tcgaccggtt tttgtgatca aagagaaaaa agttcatccg aataatcaaa
      661 aagaacaatc agaagaggaa aatttatgaa atttttagtt accggcggag ccggttatat
      721 cggatcgaca gtcgtttctg cgctcgagga tgcggggcat caagctgtga ttttagattc
      781 cttggttacc ggccgaatag aatttacccg cggaaagatt ttttatttgg gagatattgc
      841 ggatcgcaaa atcctgacgc aaatttttgc agatcatccg gatatttttg ccgtgttaca
      901 ttgtgcggct ctgattgtcg ttccggaatc cgttgaaaag ccttatgaat actatcgtga
      961 gaatgtcgca aaatccaatg aaatgtttca tatcctctat accttgggtt gccaacgtat
     1021 cgtattcagt tcttcggccg ctatttatga taaagcggaa aatttcatgg tgaccgagga
     1081 ttcgccgctg gctcccaaca gtccctattc cagaacaaaa tatatgatgg aaatgatttt
     1141 gcaggatacc tccaatgctt ataatatgcg ggggatcgca cttcggtatt tcaatccgat
     1201 cggagccgat ccgaagatgc gcagcggcat gcatgtcaag gaaccttctc atgtcctcgg
     1261 aaaattggtg gatacggcat tgggcaaaca ggatgctttc accgttacgg gtacaaactg
     1321 gccgacacga gacggctcgg gtattcgaga ttacattcac gtttgggatc tggcgcaagc
     1381 ccatgtcaaa gcgttggctg aatttgatca ggtttttgaa cgaaccggcg atccgaacat
     1441 caattttata cccattaatc tcggttccgg caaaggcgta accgtttttg aactggtgaa
     1501 tgcttttgag cgggtttatg ggaagaagat taacattcgt acagcgccgc cgcgtcccgg
     1561 agacgttgcg ggagcgtacg ccaatgcgga tcgggcaaaa aaattgctgg gttgggaaac
     1621 tcaactgagc attgaagacg gaattcgcac cgccttggag tggggaaaga tacgagagag
     1681 tattttacat tgggattaat cgcctgaact aatcaaaagg aagagagggc gctctatatt
     1741 tcgaaacaga atataagagc gccctttcga ttgaatatgg ctatatcgct tataattgga
     1801 accgacgaac agaaaggcac ggcaataacg atgcaaccaa gtttgaccca tggattgaat
     1861 attctttata ccgggaacgg gaaaggcaaa actaccgcag cgatgggatt actttttcga
     1921 gcttggggga ataatatgag tgtcgccggt attcagttca ttaaatcacc cgaaattgtc
     1981 aagggcgaga tgatcgcttg ccgacggttg ggtattcctt ttgaaacact cgggaaagga
     2041 tttgttttcg aaaaatttga tacaaccatt catgcggaag ctgcccgttc cgcttgggat
     2101 tttactcggg aagtaatcct gcatcgaaat ttggatatgc tgctgttgga tgagattacc
     2161 tatctattcg tttataaatg gctggattgt aatgaattta ttcaatggat tcgggagaat
     2221 aaacctccga ccatgcactt aattctgacc ggtcgaaacg cgccgcagga actgattgaa
     2281 tttgcggatt tggtaacaga gatgaaagaa attaaacacc cttataccac ccaacgcata
     2341 cccgcccaga tcggtattga atattaatga aagcttcgta tcgatatatc agctgacagg
     2401 aaggaaccaa tcaaatgaat ctatccaaga aaaggacgat aatttttatc atcatcacgt
     2461 ttgtgatcac atgggctgcc gaattcatgc tttggaaagg cggcggattg aaaaatccga
     2521 gagttcaaat ctacctttcc attattatga tgattccgat gatcggttct tttctgaccc
     2581 gttggattac caaagaagga ttggagaatc tatggctgcg attggatttt aaagcgaata
     2641 tcaagaccta tttgatcgct tggttcggac cggctttgtt gatcctgttc ggttcggcgc
     2701 tctattacct gattttcccg gcaaaatttg atcccgaaat gtctgtttta cggcaatcgt
     2761 tgaacgcgtt gggggaacag caaacagccg aacttacgac acagaaattg cttttatcca
     2821 tcctttcgca ggttttgttg gcgctgatca tgtcaccgat tttaaatgcg atcccatgtc
     2881 tcggagaaga aatcggttgg cgcgcttatt tattgccgaa actggcggat tttcttccgc
     2941 ggaaaaaagc ggttgtaatc agcggagttg tttggggaat ctggcatgct cccattatcg
     3001 cgatgggata taactacggg gaaaattatc cattgttccc ctggctcgga attatcggga
     3061 tgattttatt ttgtattttt gtgggttcgt ttctcgcctg gcttacattc cgaatggaga
     3121 gtgctttgcc ggctgcgatt gcccatggag ccgtcaacgg aatggcgtcc gtcgggattt
     3181 tcttcctcag aggaaccccc gaaccgtttt taggacccta tccggtggga atcgtcgggg
     3241 gattcggttt cattgttatc ggcattttat gttatctgaa tattgaaaaa gcgaagcctt
     3301 tacaatcctg atatcaattt ggttcgtttc ttgcaccatt attctacgaa ggcaatcagc
     3361 ttattttgaa tagaagctga ctggaaattg tatacgaagc agctcgatcc tgactgcttg
     3421 gattgtataa cactttgtat tatttatatg ccggaggcaa taatgatgaa aaacaaaaaa
     3481 caacttttcg ttcttatgat attggttgcg atgtttgcaa ttggtatcgc aacagttcaa
     3541 gcggaaggga ttgaacccta tccttcttac accattacca gtgtaacagt cgatcaaagt
     3601 gtcagcattt taacccttaa tttccccgct gacacagagt tcattgtccg catgaaagac
     3661 ttaaattcag tcagcgatta tattgacgtc gcaaaattca acactcaatc cgggggcagt
     3721 tttacagcca catttcctat tcccgcggaa ctggtcggga agaaaagcat cggtatgctg
     3781 ctgagcaaca ataacggaat tgaaattccc agcacattca gcaacgaagc catgacggaa
     3841 accgttgcga ccgtttgcga ttacagcgtt cttccttcct tcacaatcga cagtgtcgtt
     3901 aaaggaaaga gcatcactgt caccaccggt accttccccg ccaatcagtc cttcacggta
     3961 aaaatgggtt attatgctgc cggagctccc gctgcgcacc atcattgtgg cggacacgat
     4021 tgccccactt ccaatccttg ggatccatgg atggatgtcg atgggactcc agaaccggaa
     4081 atgtacccga ttcccgatcc atctttttct tggatgttcg gaccgatgat cccgggttgc
     4141 ggaacaagcg gctgtgttaa tttccatctt ggcggtgcaa caccgcacgc cggtttgatc
     4201 ccgacttatt tcgtcggatt cgaagtcggt acctacgatt ccgccgacgg ttcaccgcag
     4261 actgtttcct atgacattcc ggaagagttg aaagggtaca gcccgattat cgtccgattc
     4321 gaagataagg gttcctgcgg attctacggt tacaactatt tctggaatgc agatttcccg
     4381 gtcacatcat cggagtctgt gccggaagtc caaatcgaaa ccgtcagtca acaataattc
     4441 gaaaatcgat tttcaagaaa cagtattatc atataaaagc ctgcggaacc tacgaaaagg
     4501 ttcgcagact tttattttgt cttagaatag aaagattgat ttatgccgga caaaggtata
     4561 acccaaagga ggaaatcata ctttcatgag tgatgaaatc gttca
//
LOCUS       DAOMIV010000105         4587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_19280_length_4587_cov_4.244263, whole genome shotgun sequence.
ACCESSION   DAOMIV010000105 DAOMIV010000000
VERSION     DAOMIV010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19280_length_4587_cov_4.244263"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2021)
                     /locus_tag="PKV59_03610"
     CDS             complement(<1..2021)
                     /locus_tag="PKV59_03610"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQP45870.1"
                     /translation="MYRGPRIVNWSPKLQTAVSDLEVEYSEEPGTLYYFKYMLADGSG
                     DYLPVATTRPETILGDTAVAVHPEDERYQKYIGKTALVPIVGREIPIIADEYVDREFG
                     TGVVKITPAHDPNDYKIGETHHLEMINILTKDAKINEAGAPYTGMDRFECRKKIWADM
                     KEAGLVIREEPYMMNIPRSQRSGEIIEPMISTQWFLKMKPLAEPALEAVRNGSITIIP
                     DRFTKVYYNWLENIQDWCVSRQLWWGHRIPVWYCQDCGEVSASRTDLEVCPKCGSKNI
                     EQDPDVLDTWFSSWLWPFSTLGWPEETPDFKYFYPTTMMETGYDILFFWVARMIMSGL
                     YFTGKIPFSQVYLHGLVRDEHGMKMSKTKGNVVDPLTVMDQFGTDALRFTLLVGSTAG
                     NDTNLSVKKVEANRNFANKLWNAGRFVIGALDKAPSRADDGSPIQPTLADRWIWARLQ
                     NLITDVERLFDSHQYGEAGRQIYDFFWSEYADWYLEIAKIQLSRGGNTAFNTARLLVR
                     ILDTCLRLLHPFTPYVTEALWEHLKTAAIDQSEAYAPAHGWEEALIIAKWPEAESIGE
                     SDQLAVKDMLMIQDVIRAIRNIRTEKNVKPGTLIPATIVTGEKTAVFESQRDAIATLA
                     HLDPDQLEILPHLDTPPADRISTAASGIGIFLEIEEEIDGVHMHDVGVA"
     gene            complement(2021..2563)
                     /locus_tag="PKV59_03615"
     CDS             complement(2021..2563)
                     /locus_tag="PKV59_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQP45871.1"
                     /translation="MDGTNINQHELPKTYDFAANEEKLYRWWEEQGYFKPWNDPNKPD
                     FNPDHTPYVISIPPPNVTGELHLGHAMFVSMEDLMIRYHRMKGMPTLWVPGTDHAGIA
                     TQMVVERALNAQGIKREDLGREKFIEKVWEWKEKYHDRIVAQIRRLGASCDWDRERFT
                     LDEGLRKLYAKSLFDFTKTD"
     gene            complement(2993..3400)
                     /locus_tag="PKV59_03620"
     CDS             complement(2993..3400)
                     /locus_tag="PKV59_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45872.1"
                     /translation="MIWIVPLIFVLVAGYIFWWNSNENKAIPDTDAVDLFKEAQRILS
                     NSVFKRNSLFSRPTAQFGYPINAGHPNGQPPAQTNETPKEIETPGDSAQSGSTDHSSG
                     IEKLPSSSDSEEPVSHEDKSEDQNKDNSSHPDS"
     gene            complement(3397..>4587)
                     /locus_tag="PKV59_03625"
     CDS             complement(3397..>4587)
                     /locus_tag="PKV59_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HQP45873.1"
                     /translation="PVLHYNLGMNITIDTMNVTLADTPKTLAKVAAWLEKCSEVAVDT
                     ESNSLYVYYEQVCLLQASSEENHAILDTLALKDLSSIAPIFSSKSILKVFHASEYDIM
                     CLKRDFGFEFNHIFDTMIAARILGRKEVGLAAMLESELGIRLEKKYQKANWGIRPIPE
                     DMLKYAIHDTDYLIELKDKLEQELSERNLTELAQEDFDRVCKTPSGQYAPETTNWWKI
                     AGNNHQLTVQQIGMLQAVCEWRERIAKNMNIPVFKVMQNHVMLELCLNPPQSEEELAN
                     IKGMRKSLIEKEGKNLMKIFKTTDRDIRCIPRPPKTKIPCDSTLQRREHLRNWRKETG
                     IKYDVPSDVILPRDIMEEIICKNPQNEAQLQSIMVDLPWRYRQFSTEIIQLLNKLEQQ
                     KEFA"
BASE COUNT         1153 a         1093 c         1001 g         1340 t
ORIGIN      
        1 tcggcgacgc cgacgtcgtg catgtgcacg ccatcgattt cttcttcgat ttccaaaaag
       61 atacctatac cggaagccgc ggttgagatc cgatccgcgg gcggtgtatc caaatgcgga
      121 agaatttcca gttgatcggg atcgagatgt gccaatgttg caattgcatc ccgttgcgat
      181 tcaaaaacag ctgttttttc gcccgtgacg atcgtcgccg gaatcaatgt tcccggtttc
      241 acattttttt cggtacgtat attgcgaata gcccggatta catcttggat catcaacata
      301 tctttgacgg caagttgatc cgattcaccg attgattcag cttcaggcca tttggcgata
      361 atcaaggctt cttcccaacc gtgcgccggc gcataagctt ccgattgatc gatggccgcc
      421 gtttttaaat gttcccataa tgcctcggtt acatacgggg taaagggatg taacaatctg
      481 aggcaggtat ccaaaatccg caccaataaa cgagcggtgt taaatgccgt atttcctccc
      541 cggcttaatt ggattttggc gatttccaaa taccaatccg catattcgct ccagaagaaa
      601 tcataaatct gccgaccggc ttctccgtat tgatggctgt caaacaaacg ttcaacgtca
      661 gtaatcagat tttgtaaacg cgcccaaatc caacgatctg ccaaagtcgg ctggatcggg
      721 ctgccgtcgt ccgctcgact cggcgcttta tcgagcgctc cgatcacaaa tctgccggcg
      781 ttccagagct tgttggcaaa atttcgattc gcttccacct tcttgacgct caggtttgta
      841 tcattcccgg cggtcgatcc caccagtaag gtgaaacgta acgcatccgt cccgaattga
      901 tccataacgg tcaaaggatc aaccacgttt cccttggttt tgctcatttt catcccgtgt
      961 tcatcccgaa ccaatccgtg cagataaact tggctaaagg ggatcttgcc ggtgaaatag
     1021 agaccgctca taatcattcg ggcgacccag aaaaacagaa tatcatatcc ggtctccatc
     1081 atagtggtcg gataaaaata tttaaaatca ggcgtctctt ccggccagcc gagagttgaa
     1141 aaaggccaca accacgaaga aaaccaggta tccaatacat ccggatcctg ttcgatattc
     1201 ttactgccgc atttcggaca aacttccaaa tccgtccgcg aagcggaaac ttctccgcag
     1261 tcctgacaat accaaaccgg gatacgatgc ccccaccata gttggcggct gacgcaccaa
     1321 tcctgaatat tttccaacca gttgtaataa actttggtaa accggtccgg aatgatggtg
     1381 atactgccgt tgcgaacggc ttcaagcgcc ggttccgcga gcggtttcat cttgagaaac
     1441 cattgagtcg aaatcatcgg ttcgatgatt tcgccggatc gttgagaacg cggaatattc
     1501 atcatatacg gttcttctct gatgaccaac ccggcttctt tcatatccgc ccaaatcttt
     1561 tttcggcatt caaagcggtc cattccggtg taaggggcgc ccgcttcgtt aatcttggca
     1621 tccttggtca aaatattaat catttccagg tgatgagttt caccgatttt atagtcgttg
     1681 ggatcatgtg cgggagtgat tttcacaacg cccgtaccga attcacgatc gacatattca
     1741 tccgcaataa tcgggatttc tcgcccgact atggggacaa gcgctgtctt gccgatatat
     1801 ttttgatacc gttcatcttc cgggtgaacg gcgacagcgg tatcgccaag gattgtttcc
     1861 ggacgggtcg ttgccaccgg caaataatct ccgcttccgt ccgccaacat gtatttgaaa
     1921 taataaagtg ttccgggttc ttcggaatat tcgacttcca gatcggagac ggcagtctga
     1981 agcttgggag accagtttac gattctcggt ccgcgataca tcaatccgtc ttcgtaaaat
     2041 cgaacaaaga cttcgcgtac agctttcgaa ggccttcatc caaggtaaat cgttcacggt
     2101 cccaatcaca ggaggcaccg agacgacgga tctgggctac gattcgatca tgatatttct
     2161 ctttccactc ccatactttt tcgatgaatt tttctcttcc cagatcttct cgtttaattc
     2221 cctgcgcatt taaagcccgt tccacaacca tttgtgttgc gataccggca tgatcggttc
     2281 ccgggaccca aagtgtgggc attcctttca ttcgatgata gcgaatcatt aaatcttcca
     2341 tagaaacaaa catggcatgc ccgaggtgca actcacccgt cacattcgga ggagggatgg
     2401 atataacata aggagtatgg tcgggattaa aatccggttt attgggatcg ttccacggct
     2461 taaaatatcc ttgttcttcc caccatcgat ataacttttc ttcattcgct gcaaaatcat
     2521 atgttttcgg cagttcatgt tggtttatat ttgttccatc catttttcct cctattcaga
     2581 aaaaacactt tcgtccgaaa gaggacgaaa gtgtttaatt tccgcggtac cacctcaatt
     2641 cacaccggtt ccaagaaacg ggtgtgctct cttgtctgag attgataacg gttctccgcc
     2701 gtgctgttct actggctata aatcggatag cgttcttcag caaatcttat cggatgacag
     2761 ttgtcgtata tttccctcgg gattctcagc tgcagccctt ctctgtcagg gtatttcgac
     2821 aatttaatcc gacatctctt acgatatatt gaggcaaatt atacagaaag tacggcttac
     2881 cgtcaaccgg agattgatga aaggtgatac ttatttggtc gacgttttct gtggtgattg
     2941 aataccggcg gataagggat aaaagcgaaa acaaaggaat ggatatcctc aatcaggaat
     3001 ctggatggga tgaattatct ttgttttgat cttccgattt atcttcatgg ctaacaggtt
     3061 cttccgaatc cgaagaagag ggtaatttct cgattccgga tgaatgatcg gttgatccgg
     3121 attgggcaga gtcgccggga gtctctattt ctttcggcgt ttcatttgtt tgtgcaggag
     3181 gttgtccatt cggatggccg gcatttatcg ggtaaccaaa ctgagcggtg ggtcgtgaga
     3241 aaagggaatt tcgcttgaaa acggaattcg aaagaattcg ttgggcttct ttgaaaaggt
     3301 caacggcatc ggtatccgga attgctttat tttcgttgct gttccaccaa aagatatacc
     3361 ccgcaacgag gacaaaaata agcggaacaa tccaaatcat gcgaactcct tttgttgttc
     3421 taatttattc aacagctgaa taatttcggt tgaaaattgt ctgtagcgcc atggaagatc
     3481 aaccataatc gattgaagtt gtgcttcgtt ttgcggattt ttacaaataa tttcctccat
     3541 gatatcacga ggaagaatta catcggacgg aacatcatat ttgatcccgg tttcttttcg
     3601 ccagtttctc aggtgctcac gcctttgtaa tgttgaatcg cacgggattt ttgtctttgg
     3661 cgggcgggga atacaacgaa tgtcacgatc ggtggttttg aagattttca ttaaattttt
     3721 cccttctttt tcgatcaggg atttccgcat tcctttgata tttgccaatt cttcctccga
     3781 ttgcggagga ttcaagcaaa gctccagcat aacatgattt tgcattactt tgaaaacggg
     3841 gatattcatg tttttggcga tccgttcgcg ccactcgcag acagcctgca acatgccgat
     3901 ttgttgcacg gtcagttgat gattgtttcc ggcgattttc caccaatttg tcgtctccgg
     3961 ggcatattgt ccggacggag ttttacaaac ccgatcaaaa tcttcctgcg ccagttccgt
     4021 cagattccgt tcagatagtt cttgctccaa tttgtctttc agttcaatta aataatccgt
     4081 atcatgaatt gcatatttca gcatatcttc gggaatggga cgaattcccc aatttgcttt
     4141 ttggtacttt ttttccaaac gaattccgag ttcggattcc agcatcgcgg caagaccgac
     4201 ttcttttcgc cccagaatcc tcgctgcgat cattgtgtca aagatatgat taaattcgaa
     4261 gccgaagtca cgtttcaaac acatgatgtc gtattcggat gcatgaaaaa ctttcaagat
     4321 cgacttcgaa gaaaaaatcg gagcgatcga acttaagtct tttaatgcca aagtatctaa
     4381 aatcgcatga ttttcttcac tggaagcctg taataagcaa acttgctcat aatatacata
     4441 aaggctgttt gattcggtgt cgactgccac ttcggaacat ttttccaacc atgcagcgac
     4501 ctttgccaaa gttttcggtg tatccgcaag cgtgacattc attgtgtcta tggttatatt
     4561 catgcccaaa ttataatgca gtaccgg
//
LOCUS       DAOMIV010000106         4545 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_19554_length_4545_cov_4.111359, whole genome shotgun sequence.
ACCESSION   DAOMIV010000106 DAOMIV010000000
VERSION     DAOMIV010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4545
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19554_length_4545_cov_4.111359"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PKV59_03630"
     CDS             complement(<1..581)
                     /locus_tag="PKV59_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HQP45874.1"
                     /translation="MKNSVPENILVKGKELYDGIKRLGDIPEAYFDPVRRDSMERLSR
                     LKDSRKGERCFIMGNGPSLKNTDLSKLKNEYTFGLNRIYLAFPEMGFETTYYLCVNDL
                     VVEQTSDDIQKLKMPRFVTTRALKWLKPEENLYFLYSTYTGPTFATDIRKRMWEGATV
                     TYMALQTAFYLGFKQVILIGVDHNFQTKGKPNET"
     gene            complement(667..1644)
                     /locus_tag="PKV59_03635"
     CDS             complement(667..1644)
                     /locus_tag="PKV59_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQP45875.1"
                     /translation="MKHTIILSAPYMMSSVERFRKVIEGYYGINMIVPEVKQKLTEDQ
                     IISYAGQFDGTICGDDVYSARVIEACVPRLKVISKWGTGIDAIDKEAATRLGVVVGNT
                     LNAFTIPVSETAIGYMLSFFRAIPFMDDSMREGIWNKPSTTTLSENTIGIVGVGNIGQ
                     AVVRRLRAFGCRILVNDIAEIKPDFLIENRIESVSLPELLSRSDIVTMHTDLNPTSYH
                     LINRETLAMMKPTAILINTSRGPIVDEAALAEALENGKLGGAALDVFEVEPLPDDSPL
                     RKFKNVLLAPHNSNYSPMACERVHWNTIRNCLIPLGYDVSKLEELKKTI"
     gene            complement(1772..2446)
                     /locus_tag="PKV59_03640"
     CDS             complement(1772..2446)
                     /locus_tag="PKV59_03640"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HQP45876.1"
                     /translation="MKIVAFVPMRHNSVRVEGKNYRMLAGKPLYHYIMEALLAVPEIN
                     EVVVDTDSPVVMEGLTKDFPTVKSLLRPEALRADDVPMNEILIYDTSQVQADYYLQTH
                     STNPLLKSETISKGIRSFLEKTYLYDSLFSVTRVQKRFWDQLARPINHNASILLRTQD
                     LPPIYEENSCMYLFTREILMRKRHRIGDRPMMFEIPAIEAQDIDDETEFNITEMLMKL
                     RQQGKL"
     gene            complement(2459..4138)
                     /locus_tag="PKV59_03645"
     CDS             complement(2459..4138)
                     /locus_tag="PKV59_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45877.1"
                     /translation="MAVDSSKRSVFMRIRDRRRIYDTDGANSRKFHEINRYSSQRVRK
                     DEQPVLFFNASTRLTRTSLNAAFAWLAANSLQSTGVPVVFMTCMRGLNPCLLATDREN
                     PKKELPCQSCIRLSKSMYNGLQTVPLEFHEDPILFNEVEGLTYHELESYVHEGVPLGE
                     LTLPSLRWIMRRHHLEDVPNALPLQRLYIRSAWSTYLLAKEWISNNHPRAIVVFNGLQ
                     YPEATVKWIGKQNGIPTFTHEIGLMPETAFFTEGEATACPINLPADLEMTPERNKKLD
                     DYLESRMKGSFQTAGVRFWPEMEGLTPEFWKMVRMFRQVVPVFTNVVFDTSQKHANVV
                     FDNMFEWLDSVKSLIEKNADTLFVIRAHPDELRPGKESLETVTDWVVGNHLTHLTNVL
                     YIAPDQYFSSYDLIRIAKFVMVYNSTIGLEAAAMGAAVLSGGKSRYTQVPITYFPQSK
                     EAFIQKAQDMLDADEWVVPDEFQQNARKFMYYQFFHTALDFSEFLSPDPVWNGYVKLK
                     DFKPESLLPENNETMRVIQDGILRGKPFVYPHSFEKDPQLINSSVQMKNDK"
     gene            complement(4149..>4545)
                     /locus_tag="PKV59_03650"
     CDS             complement(4149..>4545)
                     /locus_tag="PKV59_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HQP45878.1"
                     /translation="DQIRQSVSLFGNLDNLIITHATSAYPCKPEELNLRMIPKLIEEF
                     DCPIGYSGHEVGLIPSVISVALGACLVERHFTLDRAMWGTDQAASVEPQGMEKLVKYI
                     RVTEASLGDGIKKVYDDELPSLKKLRRVQ"
BASE COUNT         1261 a         1096 c          903 g         1285 t
ORIGIN      
        1 gtctcattgg gttttccctt ggtttggaaa ttatggtcga caccgatcaa aatcacctgt
       61 ttgaatccca gataaaaagc cgtttgaagc gccatgtaag tcactgtcgc gccttcccac
      121 attctttttc gaatatcggt ggcaaaagtc gggccggtat aagtcgaata aagaaaatag
      181 agattttcct caggtttcaa ccatttcagt gctcgggttg taacaaaacg cggcatcttt
      241 aatttttgaa tatcgtcgga tgtttgttcg acaaccagat cattcacaca aaggtaataa
      301 gtggtttcaa agcccatttc cggaaaagcc aaataaatac ggttcaagcc aaaagtatac
      361 tcattcttca atttggaaag atcggtattt ttcaaactgg gcccgttccc catgataaag
      421 caacgctccc ctttacggct gtctttcaat cgactcaaac gttccatgga atcccgacga
      481 accggatcga aataagcttc cggaatgtca ccgagtctct ttatcccgtc gtaaagctct
      541 ttccctttca ctaatatgtt ttcgggaaca gaatttttca tattcgtttt tgatgtcgac
      601 ataactcact caataaatcg gggaaaattt gtaaatcatt ttccccgata tcggaaatga
      661 aattgcttag atcgtctttt tcagttcttc cagtttggaa acatcataac ccaaaggaat
      721 caaacagttg cgaatcgtat tccaatgaac gcgttcacaa gccatcggac tgtaatttga
      781 gttatgcgga gccaacaaca cattcttaaa cttccgcaac ggagaatcat cgggcaaagg
      841 ttccacttca aaaacatcca atgcggctcc gcccaatttc ccgttttcaa gcgcctcggc
      901 taaagcggct tcatccacga tcggaccccg tgaggtattg atcaaaatcg ctgtcggttt
      961 catcatcgcc aatgtttcac gattgatcag atgatagctg gtgggattca gatccgtatg
     1021 catggtgaca atatccgaac ggcttaacaa ttccggcaat gaaaccgact cgatccgatt
     1081 ttcgatcaaa aaatccggtt taatttcggc gatatcattt accaagatcc ggcaaccgaa
     1141 tgctcttaaa cggcgtacta ccgcttgtcc gatattcccg accccgacaa tccctattgt
     1201 attttctgac agagtggttg ttgaaggctt attccaaatt ccttcccgca tcgagtcatc
     1261 cataaaagga atcgcacgga aaaacgaaag catatatccg atcgctgtct ccgaaacggg
     1321 aatcgtaaaa gcattgagtg tatttccgac gacgactccc aatcgggtag cggcttcttt
     1381 atcgatggca tcaatgcccg ttccccattt ggaaatcact ttcaacctgg gaacacaagc
     1441 ttcgatcacg cgggcgctgt aaacatcatc cccgcagatc gttccgtcaa attgaccggc
     1501 gtaggagata atttgatctt cggttaattt ttgttttact tccggaacga tcatattgat
     1561 cccataatat ccttcaatta cttttcggaa tctttccact gatgacatca tataaggtgc
     1621 ggatagaatg attgtatgtt tcattatttt tttattctcc tgaagaaatt ttctgccaca
     1681 gaacgataac gtctgtggaa aatgcatacg gcagaaccgt cacaacatca tttttgtttt
     1741 tcagtaaaag tcattatatc ccgaaaaaaa cttacaactt tccttgttgt cgcagcttca
     1801 tcaacatttc cgtgatatta aactccgttt catcatcaat atcctgcgct tcgatggccg
     1861 gaatttcaaa catcatgggg cgatcaccga tccgatgacg tttgcgcatc aatatttcac
     1921 gagtaaacaa atacatacag gaattctctt catagatcgg cggtaagtct tgagtgcgca
     1981 gtaagatcga tgcattatga ttaatcggcc tcgctaactg atcccaaaat cttttttgaa
     2041 cgcgcgtgac ggaaaataag gaatcatata gataagtttt ttccaaaaag gatcgaattc
     2101 cttttgaaat tgtttcggat tttaaaagcg gatttgtgct gtgagtctga agataataat
     2161 ccgcttgaac ttggcttgta tcatagatta aaatttcgtt catcgggaca tcatccgctc
     2221 gtaacgcttc gggacgcagc aatgatttga ctgtcgggaa atccttggta agcccttcca
     2281 tgacaaccgg actgtcggtg tcgaccacca cttcattgat ttcaggaacc gccagcagcg
     2341 cttccataat atagtgataa agcggcttcc cggctaacat tctgtaattc tttccttcga
     2401 ccctgaccga attatgtcgc attggaacaa aagcaacaat tttcataaat tttctccttt
     2461 atttatcgtt cttcatctga acggatgaat tgatgagctg cgggtctttt tcaaacgaat
     2521 gagggtagac gaacggcttt cctcgcaaaa ttccatcttg aataacccgc atcgtctcat
     2581 tattttccgg taacagcgat tccggcttga aatcttttaa tttcacgtaa ccgttccata
     2641 cgggatcggg agataaaaac tcggaaaaat ccaatgcggt atgaaagaat tgataataca
     2701 taaatttgcg tgcgttttgt tgaaattcgt ccggaacaac ccattcgtcc gcatccagca
     2761 tatcctgcgc tttttgaatg aaagcttctt tcgattgcgg aaaataggta atcggtacct
     2821 gcgtatatcg cgattttccg ccgctcaaaa ccgccgcccc cattgccgcc gcttccaaac
     2881 cgatggtgga attgtaaacc ataacaaatt tggcgatccg aatcaaatca tatgagctga
     2941 aatattgatc cggagcgata tagaggacat tcgtcaaatg ggtcaggtgg tttccgacaa
     3001 cccaatcggt aactgtttcc aaggattcct ttcccggacg aagttcatcc ggatgcgctc
     3061 gaattacaaa aagagtgtcc gcattctttt cgatcagtga cttgaccgaa tccaaccact
     3121 cgaacatatt atcgaaaaca acattggcat gtttttgact ggtatcaaag accacgttgg
     3181 taaacaccgg tacaacttgc cgaaacatcc gcaccatttt ccaaaattcg ggtgtcaatc
     3241 cctccatctc cggccaaaag cgaacgcctg ccgtttgaaa agaacccttc atccgacttt
     3301 ccagataatc atccaatttt ttatttcttt ccggtgtcat ttccagatcg gccggcaagt
     3361 ttatcggaca agccgtagct tcgccttccg taaaaaacgc tgtttccggc attaatccga
     3421 tttcatgagt gaatgtcgga attccgtttt gttttccgat ccatttcacg gttgcttccg
     3481 gatactgtaa cccattaaaa accacaatcg cccgcggatg gttgttcgat atccattctt
     3541 tcgccaggag ataagtactc catgccgatc gtatatatag tcgttggagt ggcaaggcat
     3601 tgggaacatc ttccaagtga tggcgtctca taatccaacg tagagacggc aatgtcagct
     3661 cacccagagg gaccccttca tggacatagg attccaactc gtgataggtt aacccttcga
     3721 cttcattaaa gaggatcgga tcctcgtgaa attccaacgg tacggtttgt aacccgttat
     3781 acatgctttt actcagacga atacaacttt gacagggaag ttcttttttc ggattttcac
     3841 gatcggttgc caacagacac ggattcagtc cgcgcataca ggtcataaat acgaccggca
     3901 cacccgtaga ttgcaaacta ttcgcagcca accaggcgaa agcggcattt aaactggtac
     3961 gcgtcaatcg cgtcgaagca ttgaagaaaa gaaccggttg ttcatccttc cggactcttt
     4021 gtgaagaata acggttaatt tcatgaaatt tacgagaatt tgcgccatcc gtatcgtaaa
     4081 tgcgccgacg atcgcgaatt cgcatgaaaa cagatcgctt acttgaatcg actgccatgg
     4141 aagacaactt attggactcg gcgcaatttt ttcaggctgg gcaattcgtc atcatacact
     4201 ttcttgattc cgtcgcccaa agaagcttcc gtaacccgaa tatacttcac cagcttttcc
     4261 attccctgag gttctacgga agcggcttga tccgttcccc acattgcccg atccaaagta
     4321 aaatggcgtt ctaccagaca agctcccaat gccaccgaaa taacggaagg aatcaacccc
     4381 acctcgtgcc cagaatatcc gatcgggcag tcaaattctt cgattaattt tgggatcatc
     4441 cgcaggttaa gttcttccgg tttgcaggga taagcactgg tcgcatgagt aataatcaga
     4501 ttatccaaat tcccgaacag actcaccgat tgtcggattt gatcc
//
LOCUS       DAOMIV010000107         4523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_19710_length_4523_cov_3.879588, whole genome shotgun sequence.
ACCESSION   DAOMIV010000107 DAOMIV010000000
VERSION     DAOMIV010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19710_length_4523_cov_3.879588"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="ruvB"
                     /locus_tag="PKV59_03655"
     CDS             complement(<1..736)
                     /gene="ruvB"
                     /locus_tag="PKV59_03655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQP45879.1"
                     /translation="MDKKYLSVAKRHFSMASADIIIIMDKDRLINPGIISSDDRVDLA
                     LRPKRLNEIIGQDQIRENLSILLTAAKQRNEPLDHVLFYGPPGLGKTTLAHVLANEMG
                     VSIKVTAGPAIERAGDLAAILSNLHEGDILFIDEIHRLNRAIEEILYPAMEDSVLDIM
                     VGKGPSARSIRLKLPRFTVIGATTRLSLVSSPLRARFGAVYRLDYYDQPAMEKIIENA
                     AVKMNVTVDDGGIKEIAVRSRGTPRIG"
     gene            789..1760
                     /locus_tag="PKV59_03660"
     CDS             789..1760
                     /locus_tag="PKV59_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HQP45880.1"
                     /translation="MYGYNFAKHPVLDQKMICLWSENDHVRKTEAKLLPEFGSNLCSY
                     SVDGIEFLHQAPMPVIGSKFFGIPVLYPFPNRIRNCRFTFDGTEYVLPDNDGGRSLHG
                     LVYDTPFQFETPIVTDDDISVKTKLEILPGHPIYKLFPIANSLELTYILGADGLTIQV
                     KVSNLDQEKRFPFGFGIHPYFNVLGLKSQVKLYVPAKKWMEAVNLIPTGRLIDLDKSP
                     ADLSKPTKLSGLNLDEVWYGMEPKKPQAIIYEALGKKMVLYASDIFTHSVTYTPPFES
                     FLCVENQTNSTDCHNLYAKGFEEEAHLLILKPGESISGTIRFCVEDI"
     gene            1909..3204
                     /locus_tag="PKV59_03665"
     CDS             1909..3204
                     /locus_tag="PKV59_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45881.1"
                     /translation="MGKKKQMNSIGSGLNFGVDPEALDFSQDTWSWKGDIPNHPIQSD
                     GFSYDIVDENEIDRNAISLGIIGMIIIVYNAKSAKIKEEDVLPAFREVIGTRNWNTIS
                     EKEANQMAKTAISKLVDEETSGDVFDLASYAPLEIKRVLLRKLFVIARYRLDPEYRTE
                     AERRITEDIVPSIFADPQIELALLTGLIVKPKPKQEDKYTRQEEKAEESESVPSEEVS
                     EIKGEEELIIPDRDAEEALQERLKKLSKDQPVKKAKKEKPTTEKPEEEYIPFSDMEKE
                     LAGSSSPAAEPFNDGFDEFNKPPIAPAEKIPAVKTGSQTQQPKPSQVPPPPMPNIPRP
                     PIPPVPGTSHYPQTRPESSGYPSYPQPGESRSGYPAQSGPGTFPYPPQPQQPPTRGAY
                     PPPPYHAPYPQGNEMPPQQPPYTEPPAIEHTQPRKPKSR"
     gene            complement(3294..4358)
                     /gene="hypE"
                     /locus_tag="PKV59_03670"
     CDS             complement(3294..4358)
                     /gene="hypE"
                     /locus_tag="PKV59_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HQP45882.1"
                     /translation="MTGLNISEGFTCPAPIQAYDRIVSGHGSGGKMTADLIEKTFRKY
                     LESDGLAQGNDAANLPISRQAKRIAVTTDSHIVSPLFFPGGDIGALSVCGTVNDLLTS
                     GAVPQWLTAGFILEEGFSLSDLEKILASMRERSEEAGIQIVAGDTKVVEHGKGDGIFM
                     NTSGVGWIIDDFQPGGEKAQTGDTIILSGTIGDHGMAVLCARGELGFESDIRSDVAPL
                     NEIVNSLRRAAPHTHVLRDPTRGGLSTTLNEIALQSHVSVEIDETAVPIRPEVRSICD
                     LLGFDPLYVANEGKLVIFVPEVEAQDALTAIRASRYGENAAVIGRVIHGDRSPVTLRT
                     AFGTGRILESLPGEMLPRIC"
     gene            complement(4345..>4523)
                     /locus_tag="PKV59_03675"
     CDS             complement(4345..>4523)
                     /locus_tag="PKV59_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HQP45883.1"
                     /translation="HESDVCIAGAILRGLKTPPECPAFAASCTPEHPLGAAMVSGEGT
                     CSAYYRYRRNHDRA"
BASE COUNT         1239 a         1103 c         1088 g         1093 t
ORIGIN      
        1 agccgattct gggtgttccg cgcgaacgga cggcaatctc tttgatgcca ccgtcgtcaa
       61 cggtcacatt catcttaaca gctgcattct cgatgatctt ttccatcgcc ggctgatcat
      121 aataatccaa gcgataaacg gcgccgaaac gagcccgcag cggcgaactg accagcgaaa
      181 gacgggtggt ggcaccgata acggtaaaac gtggaagttt taaacgaatc gaacgggcgg
      241 aaggtccttt gccgaccatg atatccaaaa ccgaatcttc catcgccgga taaagaattt
      301 cttcgatggc gcgatttaag cgatgaattt catcgatgaa taaaatatcc ccttcatgaa
      361 gattggaaag aatagcagcc aaatccccgg ctcgttcgat tgccggaccg gcggtaacct
      421 taatgctgac acccatttca ttggccaata cgtgcgccag tgtggtcttt cccaaacccg
      481 gcgggccgta aaataaaaca tgatccaaag gctcattccg ttgcttggcg gcggtcaata
      541 atatggaaag attttcacga atttgatcct gcccgataat ttcatttaag cgcttcggac
      601 gcaacgccaa atccactcgg tcatcggatg agatgatgcc cggattaatc aaccgatctt
      661 tatccatgat tataattata tcagcggaag ccatcgagaa atggcgtttt gcgacagaaa
      721 gatacttttt atccattcgg accttgccag tgttttcggg gtcatacaat cggaaaggaa
      781 caatactaat gtacggttac aattttgcga agcatccggt attggatcaa aaaatgatct
      841 gcctttggtc ggagaatgat catgtgcgga agacggaagc caagttatta ccggaattcg
      901 gatccaattt gtgcagttac tcggtggatg gaattgagtt tctgcatcaa gctccaatgc
      961 cggtgatcgg gtcaaaattc ttcggcatac ccgttttata tccgtttccg aaccgcattc
     1021 ggaattgccg gttcactttt gacggaacgg aatatgtgtt gcccgataac gacggcggtc
     1081 gttctctgca cggacttgtt tatgatacgc cgtttcaatt tgagacaccg atcgtcacgg
     1141 acgatgatat ctctgtgaaa accaaattgg aaattttacc cggacatccg atctataaac
     1201 tgtttccgat tgcaaacagt ctggaactca cctatatttt gggcgcggac ggattgacca
     1261 ttcaggtgaa agtatccaac ttggatcaag aaaaaagatt tcctttcggt ttcggaattc
     1321 acccttattt taatgtgctc ggtttgaaga gtcaggtgaa gttatatgtt ccggcgaaga
     1381 aatggatgga agcggtcaat ttgattccca cgggcagatt gatcgacttg gacaagagtc
     1441 cggctgattt aagcaagcct acaaagctca gcggattgaa tttggatgag gtgtggtacg
     1501 gaatggaacc gaagaaacct caggcaatca tttatgaagc gctcggcaaa aagatggttt
     1561 tatatgcgtc ggatattttc acccacagcg tcacttatac gccgccgttc gaatcgtttc
     1621 tgtgtgtcga aaatcagacc aattcaaccg attgtcataa tctttatgcg aaaggcttcg
     1681 aagaggaagc gcatttattg attctgaaac ccggtgaaag tatcagcgga acgatccggt
     1741 tttgtgtgga agatatctga aatccgattg ttcggtaaat tttgctgatc gatatttacc
     1801 ggatgagttg ttcggaaaag tgaaaatcag cggaagattt atttgttaca attagaaaaa
     1861 ccggcaggat tgaaaaatat tgagaaatcc gctgattaag aggtaaatat ggggaaaaag
     1921 aaacaaatga attcaatcgg ctcgggattg aattttgggg tagacccgga agcacttgat
     1981 ttttcacagg atacctggtc gtggaaaggt gatattccca atcatccgat tcaatccgat
     2041 ggcttttcct atgatattgt cgatgagaac gaaattgatc gcaatgcgat ttctctcggc
     2101 atcatcggaa tgatcattat cgtatataac gcaaagagcg caaaaattaa agaagaggat
     2161 gtccttccgg cttttcgtga agttatcggt acccgcaact ggaatacgat ttccgaaaaa
     2221 gaagccaatc agatggctaa aaccgctatt tcgaaattag tggacgaaga aacgagcgga
     2281 gacgtatttg atttggcatc ctatgccccg cttgaaatca aacgggtatt actgcggaag
     2341 ctttttgtga tcgcgcgtta tcgattggat ccggaatatc ggaccgaagc cgaacgccga
     2401 atcacggaag atatcgttcc gtccattttt gctgacccgc aaattgaatt ggcgctatta
     2461 accggattga ttgtcaaacc gaaacccaaa caggaagaca aatatacgcg gcaggaggaa
     2521 aaagccgaag aaagcgaatc cgttccttcc gaagaagtat ccgaaatcaa aggtgaagaa
     2581 gaattgatca ttccggatcg ggatgcggaa gaagcgttac aggaacggct aaaaaaactg
     2641 tccaaagatc agcctgttaa gaaagccaaa aaagaaaaac cgacaacgga aaaaccggaa
     2701 gaagaataca ttccgttttc ggatatggag aaagaattgg ccggatcatc ttcacctgct
     2761 gcggaaccgt ttaatgatgg tttcgatgaa ttcaataaac cgccgatcgc tcccgccgaa
     2821 aagattcctg cggttaaaac cggcagccaa acccaacaac cgaaaccttc acaggttcca
     2881 ccgcccccca tgcccaatat tcctcgtcct ccgatcccgc cggtgcccgg aacgtctcat
     2941 tatccgcaga cgcgaccgga atcgagcggt tatccttcct atccgcaacc gggggagagt
     3001 cgctccggat atccggctca atccggtccg ggaacttttc cttatcctcc gcaacctcaa
     3061 cagccgccga cacgcggtgc ttatcctccg cccccttatc acgcgccgta tccgcaggga
     3121 aatgaaatgc cgccgcaaca gccgccttat acggagccgc cggcgattga gcatacccaa
     3181 ccccgaaagc cgaaatccag ataagagttt acggataaac aaccgccccg aattttttcg
     3241 gggcggttgt ttatccgtat tttgcatgtc cgtctttcgc cgatagtatc caatcaacag
     3301 attctcggca gcatttcacc cggcagagat tcaagaattc gaccggtacc gaaagcggtt
     3361 cgaagcgtta ccggcgaccg atcgccgtgg atcacacgtc cgattacggc ggcattttct
     3421 ccgtagcgag aggcgcggat tgccgtaaga gcatcctgcg cttcgacttc gggaacaaaa
     3481 atgaccagtt ttccttcatt ggcaacatac agaggatcaa atcccaataa atcgcaaatc
     3541 gatcttactt cgggacgaat cggaacagcc gtctcatcga tttcaaccga tacatggctt
     3601 tgaagagcaa tttcattcaa cgttgtactt aaccctccgc gggtcggatc ccgtaaaaca
     3661 tgggtatgcg gagcggctcg gcgtaaagaa ttcacgatct cattcaaagg ggcgacatcg
     3721 gaacgaatat cggattcaaa acccaattca ccgcgagcgc acagcactgc catcccgtgg
     3781 tcaccgatgg ttccgctcag aataatggta tctcccgttt gcgctttttc gcctcccggt
     3841 tggaaatcat caatgatcca accgacaccg ctggtgttca taaaaattcc gtctcctttg
     3901 ccgtgttcga cgactttggt atcaccggcg acgatttgaa ttccggcttc ttccgaacgt
     3961 tctcgcatcg atgccaatat tttttcaagg tccgaaaggg agaagccttc ttccagaata
     4021 aatcccgcgg ttaaccactg cgggaccgca cccgaggtta gcaaatcatt aaccgtaccg
     4081 caaacggaca gcgctccgat atctccgccg gggaaaaaaa gcggagaaac aatatgagaa
     4141 tcggttgtca cggcgatcct tttcgcctgt cgggagatcg gcagatttgc ggcgtcattc
     4201 ccctgcgcca atccgtcgct ttccaagtat ttgcggaatg tcttttcgat cagatcggcg
     4261 gtcatttttc cgccgctgcc atgcccggac acaatccgat catatgcttg aatgggggcg
     4321 gggcaggtga atccttccga aatgttaagc ccggtcatga tttctccggt aacgataata
     4381 agcggaacag gtcccctcgc cggagaccat cgcggctccc aaagggtgtt ccggcgtgca
     4441 agaagcggca aacgcgggac attccggtgg cgttttcaaa ccccgcagaa tggccccggc
     4501 gatgcagacg tcggattcat ggg
//
LOCUS       DAOMIV010000108         4517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_19742_length_4517_cov_3.880099, whole genome shotgun sequence.
ACCESSION   DAOMIV010000108 DAOMIV010000000
VERSION     DAOMIV010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19742_length_4517_cov_3.880099"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKV59_03680"
     CDS             <1..472
                     /locus_tag="PKV59_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45884.1"
                     /translation="NENWQLFLEGSLHIIVTKLTTFRHSQNADIDAIPLPLFNGSNSY
                     PLVNTWNVVLSNSNPEIQNLAVKLAEKFADPRFNDQWTQSAGYLPVRSIGQTLWQNDS
                     FYEKMITICENGELIQTDQILNKITPVINEAVSSVIKTGATPEDAAEKAIDAIN"
     gene            485..835
                     /locus_tag="PKV59_03685"
     CDS             485..835
                     /locus_tag="PKV59_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45885.1"
                     /translation="MSKNSKSQRRRFNWILFLIICILVVFAFILSESDKIEHASQTSR
                     TIQMGLLQTAEPTIILPTPTPFPSDYPKLPAEYFENEDQTDGVILGGTVLVLIILIGT
                     MVFIQKEHQKKNEK"
     gene            825..2183
                     /locus_tag="PKV59_03690"
     CDS             825..2183
                     /locus_tag="PKV59_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQP45886.1"
                     /translation="MRNKNRPLFYENKKKTWPYVVFILITIVILIIWGAQVPVIRQTL
                     GRSVLDGISYGKSLILPVSTVNPLNYRIQTYYPTLDLPSKEVIFAENLEDINSSEPYL
                     EITFTPTPTPGVLWVIEDPEKSSTNDSATLSEEVNDRLEMPIFEMADYLNDGPASLST
                     FLRFYEKPESQYLVSEKIKPDYFDPNVSLNEMLSYSEQKYPDLSGIIRENGNIQILAS
                     LLDSHFPVLIRLQYQRQQSAWKGDDLWDARYVVISGIDSKNKIVYFSDPLKGNEQTLS
                     FDRLMEDWYPFRREYLVIYPVDREESLALLLNSDWDEEENSKRALEKFQTDVTMVPDN
                     QFAWFNAATLLIENNHNEEAWDSFRTALQVGIPQRYLLYDFSLYDAAFKNGLAEELRD
                     RTAAMLKINNHSEENWLWNGWAYTLLQDKNQAEKCFRKVLDINPNNSDGQYAMEYLKQ
                     YE"
     gene            2204..3613
                     /locus_tag="PKV59_03695"
     CDS             2204..3613
                     /locus_tag="PKV59_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45887.1"
                     /translation="MEKKNNNKTIIIVVITVIVLIGCLCLSAAALGVYFLNHRIDKNN
                     EELIYETAVPETSPEDQTALPEVTPDNETDGTEINNTEITQKQQEIIKRAEKIRGLKA
                     QGDFNITFLTKEELRDQLINDMYQDTTKQDFQDEHDILTLLGFIPAEMDLESFYLDFY
                     TEQIAGYYDEDANVMYLIKGGSETDNSLTLAHEYTHFLQFDHFNPAKTLNYNDEYCEK
                     NQEYCFVLGSVFEGDATLTEMLLAAQPDLGLYDERMVEQDTSVFDNAPQYFQNYLLFP
                     YTYGFDFVSSVYRKGGFSAVNDLYSNPPLSAEQIMHPEKYSADQPVNVLFEPFQRNLD
                     ESCDLVYDNVLNEADLLWLLNSGYEKSWRISDKSAKKAAAGWGGGAFQFARCDGKPMF
                     FGKTVWDTVNDAKEFSQSLIDYNDKRWGKSKINGSWTGTDGEQIYVSVQDDKVFYMIA
                     PAEFSPDSLIDLIETGQGL"
     gene            complement(3695..>4517)
                     /locus_tag="PKV59_03700"
     CDS             complement(3695..>4517)
                     /locus_tag="PKV59_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQP45888.1"
                     /translation="KPLNETDLNIILNRALSDPERGYGKLNVKLDDDARRLLLDLAGG
                     DARNLLNGLELAVESTKPDENGIIHITLKIAEESIQKRALQYDKNGDMHYDTISAFIK
                     SVRGSDPDAALYWAAKMLLAGEDPHFILRRLIVLASEDIGLADPNALQVAVAAATAFD
                     YIGMPEGYYPIGEAILYLATAPKSNSVGAIFKAMDHINEQGTGQVPLPLMDASRDAKG
                     LGHGQGYLYPHEFPNHYVKQQYLPDGVKTDFYHPGEQGYEKKIKEWMEYLKKNSE"
BASE COUNT         1335 a          990 c          939 g         1253 t
ORIGIN      
        1 gaatgagaat tggcaattgt ttttggaagg atctttacat attatcgtca ccaaactgac
       61 caccttccgg cacagccaaa atgccgatat cgatgccatc ccgcttccgt tgtttaacgg
      121 aagcaatagc tatcctctgg taaatacatg gaatgtggta ctcagcaaca gcaatccgga
      181 aattcagaat ctggcggtaa aactggctga aaaatttgcg gatccgcgct ttaatgatca
      241 gtggactcaa tccgccggat atcttcctgt ccgctctatc ggacagactc tatggcaaaa
      301 tgacagtttt tacgagaaaa tgatcacgat ttgtgaaaac ggagaattga ttcaaaccga
      361 ccaaattttg aataaaatca cgcccgtcat caacgaagca gtatcctccg tcattaaaac
      421 cggagcgact cccgaagatg ccgccgaaaa agcaatcgat gcaatcaatt aagttgggat
      481 gaacatgtca aaaaactcga aatcgcagcg aagaagattt aattggatcc ttttcctcat
      541 tatttgtatt ttggtggttt ttgctttcat cctctcggaa tcggataaaa ttgaacatgc
      601 ttcccaaaca tcgcgaacga ttcaaatggg tttattgcaa accgccgaac cgacaatcat
      661 tttaccgact ccgactccat tcccctcgga ttatccgaag cttccggctg aatattttga
      721 aaacgaagat cagacagacg gcgtaatctt aggcgggacg gttctggtct tgattattct
      781 catcggaaca atggtcttca tccaaaaaga acatcaaaaa aagaatgaga aataaaaatc
      841 gtcctctttt ttatgagaat aaaaaaaaga cctggcccta tgtcgttttt atcctgataa
      901 ccatcgttat cctgatcatc tggggagctc aggtgcccgt tattcgacaa accttgggac
      961 gatctgtttt ggatggcatc tcttacggaa aaagcctgat tttgcctgtc agtacggtta
     1021 acccccttaa ttatcgcatt caaacctatt atccgaccct tgatctgccg tcgaaagaag
     1081 tgattttcgc cgagaatctg gaagatatca attcatccga accttatctt gaaattacct
     1141 ttacacccac accgacaccg ggtgttttat gggtcatcga agatcctgag aaatcaagta
     1201 ccaatgattc ggcgacactt tccgaggaag tgaatgatcg actcgaaatg ccgatatttg
     1261 aaatggcgga ttacctgaat gacggaccgg cttctctcag cacattcttg agattttatg
     1321 agaaaccgga aagtcagtat ttggtgagtg aaaaaataaa gccggattat tttgatccca
     1381 atgtatctct caatgaaatg ctgtcttaca gcgagcaaaa atatcccgat ctttccggta
     1441 tcatacggga aaacgggaac atccaaattc tggcttcttt actcgatagc cattttccgg
     1501 tgctgattcg actgcagtac caacgacagc aatccgcctg gaaaggcgat gacctttggg
     1561 acgctcgtta tgttgtaatt tccgggatcg attcaaaaaa caaaatcgtt tatttttccg
     1621 accctttaaa agggaacgaa caaacgcttt cttttgatcg attgatggaa gactggtatc
     1681 cgttccgtag agaatatctg gttatctatc ctgtcgatcg cgaagaatcc ctcgctctat
     1741 tgttgaattc ggactgggat gaagaggaaa attcaaaaag ggctttggag aaatttcaga
     1801 cagatgtgac catggttccg gataatcaat ttgcctggtt caatgccgct actttgctga
     1861 ttgagaataa tcacaatgaa gaagcgtggg attcttttcg taccgcttta caagtcggca
     1921 ttcctcagcg ttatctttta tatgattttt ctctgtacga tgcagctttt aagaacggat
     1981 tggctgaaga attacgtgat cgcacagctg ccatgctaaa aatcaacaat cattcggaag
     2041 aaaattggtt atggaacggt tgggcttata ctttattaca agataaaaac caagcggaaa
     2101 aatgctttcg aaaagtcttg gatattaatc cgaacaacag cgacggacaa tatgcgatgg
     2161 aatacctcaa acagtatgaa taaagaaatg ggaaggactc aaaatggaaa agaagaataa
     2221 caataagacg ataataattg ttgtcattac cgtcatcgta ttaatcggtt gtctctgtct
     2281 gtcagccgct gctctcggag tctatttcct caatcaccgg atcgacaaaa ataatgaaga
     2341 acttatttat gaaaccgccg ttcccgagac ttcacccgaa gatcaaaccg cacttcccga
     2401 agttacccct gacaacgaaa cagacggaac cgaaatcaat aatacggaaa ttacccaaaa
     2461 acaacaggaa attatcaaaa gagccgaaaa gatacgggga ttaaaggcac aaggtgattt
     2521 caatattact tttctgacaa aagaagaatt acgagaccaa cttattaatg acatgtatca
     2581 ggatacgacg aagcaggact tccaagacga gcatgatatt ttaacgcttc tcggttttat
     2641 tcccgcagaa atggatcttg aatcatttta cctggacttt tatacggaac aaatcgcggg
     2701 atattatgac gaagatgcaa acgtaatgta cttaatcaaa ggaggatccg aaaccgataa
     2761 ctcattgact ttagcccatg aatatactca cttcctgcaa tttgatcatt ttaatcctgc
     2821 gaaaactctg aattacaacg atgaatattg tgaaaaaaat caggaatatt gttttgtgtt
     2881 gggatctgtt tttgaagggg acgcaacatt gacggagatg cttttagccg cacaacccga
     2941 tttggggtta tatgatgagc gcatggttga acaggatacg tccgtttttg ataatgcgcc
     3001 gcaatatttc caaaattatc ttcttttccc ttatacctat ggatttgatt ttgtttcttc
     3061 ggtttatcga aagggcggtt tttcggctgt gaatgatttg tattcgaatc cgcctctcag
     3121 cgccgaacaa atcatgcatc ccgaaaaata ttccgccgac cagccggtta atgttctgtt
     3181 cgaacccttt caaaggaatc tcgatgaaag ttgtgatttg gtctatgaca acgtcttaaa
     3241 tgaagcggat ttattatggt tgctgaacag cggctatgaa aaaagttggc gaatttccga
     3301 taaatccgct aaaaaagccg cggcaggatg gggtggcgga gctttccagt tcgcacgatg
     3361 tgacggcaaa ccgatgtttt tcgggaaaac cgtctgggat accgtcaacg atgccaagga
     3421 attttctcag agtctgatcg attacaacga caagcgctgg ggaaaaagta aaataaacgg
     3481 ctcttggacc ggaactgatg gagaacaaat ttatgtttcc gttcaggatg acaaggtgtt
     3541 ttatatgatc gctccggccg aattttcacc ggattcattg atcgacctga ttgaaaccgg
     3601 tcagggatta taaaacggaa aaagcggaaa atatttccgt ttcatccata atccgaatca
     3661 aataatcaac atttcgagaa gaaaagaatt cggcttactc cgaattcttt ttgagatatt
     3721 ccatccattc tttgattttc ttttcatagc cttgttcgcc gggatgataa aaatccgttt
     3781 tcaccccgtc ggggaggtat tgttgtttca cataatgatt gggaaactca tgcggatata
     3841 aatatccttg tccgtgtccc aaccctttcg catctcggct ggcatccatt aacggtaaag
     3901 gaacctgccc ggtgccttgc tcatttatat gatccatcgc cttgaaaatc gcgcccaccg
     3961 aattagattt cggtgcggtc gccaaataga ggattgcctc tccgatcgga tagtatcctt
     4021 ccggcatacc gatatagtca aaagcggtag ccgccgccac tgccacctgc agcgcattgg
     4081 gatcagccag tccgatatct tcgctggcta acacaatcaa tctccgtaaa ataaaatgcg
     4141 gatcttctcc cgccaacaac atttttgccg cccaatacaa agccgcgtcg gggtcgctgc
     4201 cgcgaacgga tttgataaat gccgaaatgg tatcgtagtg catatcgccg tttttatcat
     4261 attgaagcgc ccttttttgt atggattctt cggcgatttt gagcgttata tgaatgatgc
     4321 cgttttcatc cggcttggtg ctttcgaccg ctaattccaa accgttcaaa agattccgcg
     4381 catcccctcc ggccaaatcc aacaacaacc ggcgtgcatc atcatccaat ttcacattca
     4441 gctttccgta tcctctctcc ggatccgata aagcccgatt caaaataatg ttgagatcgg
     4501 tttcattcaa cggtttc
//
LOCUS       DAOMIV010000109         4503 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_19819_length_4503_cov_3.888264, whole genome shotgun sequence.
ACCESSION   DAOMIV010000109 DAOMIV010000000
VERSION     DAOMIV010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19819_length_4503_cov_3.888264"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="PKV59_03705"
     CDS             complement(<1..685)
                     /locus_tag="PKV59_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HQP45889.1"
                     /translation="MIGTIINVAGILVGTFFGLFFGKRINEQIRNTIISGIGIFTLFL
                     GLSMFLKSEDSVVCLIGLVIGTIIGELTNIERGLENLGGWFQSFVDKNTRSDYGKDRQ
                     KFIQAFLTATMLFAIGPVSILGSIQDGLTGDYQLLLIKSILDGVTSIVFATTMGIGVA
                     FSVIPIFIYQGTITLLAEQAQSIMNDSMIAGMTAVGGVMLGAIAISSLLNIKKIRVSS
                     MIPALFITPF"
     gene            complement(766..2460)
                     /locus_tag="PKV59_03710"
     CDS             complement(766..2460)
                     /locus_tag="PKV59_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP45890.1"
                     /translation="MKKIRWQLVIIFLTGLVVGILLLSDTSNGPIQMFESQPTMGGIY
                     REGLVGNIQRMNPLLSFYNNADQDVCRLVYSGLIKFDNRGLPVSDLAAGWAISMDGTS
                     YNFALNPNAKWHDGQPVTADDVVFTYEMLKKGNGYIPDDLIDFWNKIQIKAIDSQTVQ
                     FVLASAYAPFLDYLSVGILPKHIWGNMTFEQMVDSKVNIQPIGSGPFILDDLILNGSN
                     ITGIILKNNALYYGKKPYLEEIHINYYQDSILAFQAYQQGLVDGISYISEDVLESALT
                     EPDLALYTSRLPIQTMVFMNLDNGNVDFFQDKNVRKAMLMGLNRSKIVNNILHGQAII
                     AHGPILYGNWAYYDNIPKVEYDSYKAKELLKSSGYILANEQDQVRSKDGKTLNFTLLY
                     PDDDKHRQIAESIQQDWAAINILVNLEAVPYDELINVRLEERDYEAALIDLNLSDFPD
                     PDPYPFWDQTQVSTGQNYSQWNNRTISQYLETARVETDQAERERLYRNFQVVFAEELP
                     ALPLFNPVYNFAVDKQVKGVSIGPIYRTADRFQNIESWYLVSRTTLSDEFTGKNSE"
     gene            complement(2594..2815)
                     /gene="secG"
                     /locus_tag="PKV59_03715"
     CDS             complement(2594..2815)
                     /gene="secG"
                     /locus_tag="PKV59_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HQP45891.1"
                     /translation="MKNFLDIALIITSIATVICVVLQNRGSGLGSIAGGEPGAVFSAR
                     RGIEKVLFYMTIGFSALLIIFVLVRILAF"
     gene            2912..3976
                     /locus_tag="PKV59_03720"
     CDS             2912..3976
                     /locus_tag="PKV59_03720"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HQP45892.1"
                     /translation="MEEITVNLHNHSDISDGTGNYYEIAQAGLNAKIDVLIITDHNVV
                     VRDKDSYYYREGNKLLLLIGEEINDPIGVPGNHLIILNSSREYASAASDPQTVIDSVN
                     KEGGITILAHPFENAKKQLHMKSFPWSDWSVHDFSGLEIYNFMSQFKCGVTNFFSLPN
                     HYLRPINFQMGADENTLAKWDELLCMGYRVSGFAGSDSHRFQKKVGPKTFKIFDYELT
                     CRALNNHVYIPKPLVGDVEEDKKVIYAALRNGCFFVGADMITSTAGFSFRADTETGIY
                     YPGARIPIGNSATLKIRIPEKSICRLISNGKVYKQWESVQSVPITITEPGYYRVECYL
                     PYKNKLRGWIYSNPIYFFKG"
     gene            3985..4485
                     /locus_tag="PKV59_03725"
     CDS             3985..4485
                     /locus_tag="PKV59_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQP45893.1"
                     /translation="MTTTNNVIRFLSSKKIDFQIFESEPIRRSAMETAALYHLDPMIV
                     YKTIVAFNPIRKKYLLCIIPAPYEVNLKKLASAIGEKKAAIVTQHEAEVLTGLEAGGI
                     SPFALYHKGFQIWVHETIRDQEKISVSGGQRGLSILLSTSDFIQILQPKIADIADIQQ
                     DIQSSE"
BASE COUNT         1391 a          909 c          867 g         1336 t
ORIGIN      
        1 gaaaaggggt tataaaaaga gcaggaatca tgctggatac acgaattttt ttgatattta
       61 ataagctcga tattgcgatt gccccgagca taacgccgcc taccgctgtc attccggcaa
      121 tcatggaatc attcataatc gattgggctt gttccgcaag aagtgttatc gtcccttgat
      181 aaatgaaaat cgggattacc gaaaaagcaa caccgattcc catggtggtt gcaaatacaa
      241 tagaggttac cccgtctaat atggatttaa tcaataacaa ttgataatcg cctgtcaaac
      301 catcctggat ggatcccaaa atactgaccg gtccgatcgc aaaaagcatt gtggcagtca
      361 gaaatgcctg tataaatttt tgtcgatcct ttccgtaatc cgatcgggta tttttatcta
      421 caaaagattg aaaccaacct cctaaatttt ccaatcctct ttcgatattc gttaattctc
      481 cgatgattgt tccgatgact aaaccgatca ggcacactac actgtcttcg gatttcagaa
      541 acatgctgag acctaaaaaa agagtaaata ttcctatccc ggaaataatg gtatttctga
      601 tttgttcatt aatccgtttc ccgaaaaaaa gaccgaagaa ggttccgaca agtattcctg
      661 caacattaat aattgttccg atcatagaat tattttccga atataagttt atttacgaaa
      721 tcgaatcgat caaatgattg tttttctttc cgaataaatt ggatgttatt cggaattttt
      781 cccagtgaat tcatccgaaa gtgtcgttct tgaaaccaga taccaggatt ctatattttg
      841 gaatcggtct gctgtacgat atatgggacc gatactgact cccttaactt gtttgtctac
      901 cgcaaagtta taaaccgggt tgaacaaagg cagagccggt aactcttccg caaaaaccac
      961 ctgaaagttt cgataaagcc gctctctttc ggcttgatct gtttctacgc gagcggtttc
     1021 caaatattga ctgattgttc ggttattcca ttgggaataa ttttgaccgg tcgatacttg
     1081 agtctggtcc caaaaaggat aaggatcagg atccggaaag tcggataaat tcaaatcaat
     1141 caaagcagct tcataatccc gctcttccaa tcgaacattg attaattcgt catatggaac
     1201 agcttccaga ttcaccaaaa tattaattgc cgcccagtct tgttgaatac tttcggcaat
     1261 ttggcgatgc ttgtcatcat cggggtataa caacgtaaag ttaagcgttt tcccgtcctt
     1321 tgaacgaact tgatcctgct cgttcgcaag aatgtatccg gaagatttta acagctcttt
     1381 ggctttgtaa gaatcgtatt cgactttcgg gatgttatcg taatatgccc agttaccgta
     1441 cagtatcggt ccgtgagcaa tgattgcctg tccgtgaagg atgttattca cgatttttga
     1501 gcgattcaac cccattaaca ttgcctttcg aacattttta tcctgaaaaa aatcgacatt
     1561 gccgttatcc aaattcataa aaaccatagt ttgaatcggt aatctggacg tatatagcgc
     1621 taaatccggt tctgttaaag cggattcgag tacatcctcg gaaatgtagc tgattccgtc
     1681 aaccaatcct tgctggtatg cctgaaaagc caaaatgctg tcttgataat aatttatatg
     1741 gatctcttcc aagtatggct ttttcccata atagagagcg ttatttttta aaataattcc
     1801 ggtaatatta cttccgttca gaatcagatc atccaaaatg aaaggaccgc tcccgatcgg
     1861 ctgaatgtta acttttgaat ctaccatctg ttcaaaagtc atatttcccc agatatgttt
     1921 tggcaggatg ccgacagata aataatccaa aaacggagca tatgccgatg cgaggacaaa
     1981 ttgaactgtt tgggaatcga ttgctttaat ttgaattttg ttccaaaaat cgattaaatc
     2041 atccggaata tatccgttcc ccttttttaa catctcatag gtaaaaacca catcatcagc
     2101 ggtaacgggt tggccgtcgt gccattttgc attcggattc aatgcgaaat tataggaagt
     2161 gccatccatt gaaattgccc agcccgctgc aagatccgaa acgggtaatc ctctgttatc
     2221 aaattttatc aacccgctat aaactaatcg gcagacgtct tggtccgcat tattgtaaaa
     2281 agacagaagg ggattcattc gctgaatatt tcccaccaag ccttctcggt aaataccacc
     2341 catcgtcggt tgcgattcaa acatctgaat cggtccgttt gatgtgtcac tgagaagtaa
     2401 aatgcctact accaatccgg tcaggaaaat aattaccaac tgccatctga tttttttcat
     2461 aagactattt tacctgtatc ggctatgaat ctcattactt tttatctttt gattaatatt
     2521 ttgtgagtaa aagtctaaaa atcgaaaaaa agggtgtctc tcgatatttg agacaccctt
     2581 gaacctgagt gaattagaaa gcaagaattc tgacgagaac gaaaattatt aaaagggcgc
     2641 taaatccgat ggtcatgtaa aaaagtactt tttcaatccc tcttcgggca gaaaaaacgg
     2701 caccgggttc tccacccgca atacttccca aaccactgcc gcggttctgt aatacgacac
     2761 aaatgaccgt cgctatcgaa gttataatga gcgcaatatc taaaaagttt ttcattcatc
     2821 ccctcccgca aaatcttaac ggtatgatta tatctatatt acatgaaaac ggcaagaaga
     2881 atgagagagg ttgtgttatt gtccggcgca aatggaagaa atcacagtaa acttacataa
     2941 tcattcggat atttcagatg gtacgggaaa ttactatgag atcgcacaag caggtttaaa
     3001 cgcaaaaatc gatgtcctta tcattacaga tcataatgta gtcgttcggg ataaagattc
     3061 ctattattac cgggagggaa ataaattatt attgttaatc ggtgaggaaa taaatgatcc
     3121 tatcggagtc cccggtaatc acttgattat tttaaacagt tcacgagagt atgcatccgc
     3181 tgcatccgat ccgcaaacag tcatcgattc ggtcaataaa gaaggtggga taacaatact
     3241 ggctcatcct ttcgaaaatg caaaaaagca gttgcatatg aaaagttttc cttggtctga
     3301 ctggagcgta catgattttt cgggtttgga aatatacaac tttatgagcc agttcaaatg
     3361 cggtgtcaca aatttctttt cattaccgaa ccactactta cggccgatta atttccagat
     3421 gggagcggat gaaaacacac ttgcaaaatg ggatgaatta ttatgcatgg gatacagagt
     3481 cagtggattc gcaggatcgg attctcatcg ctttcaaaaa aaagtcggtc cgaaaacttt
     3541 taagatcttt gactatgaat tgacctgtcg tgcattaaat aaccatgttt atattcccaa
     3601 accattagtc ggtgatgttg aagaagacaa aaaagttatt tatgcagcct tacggaatgg
     3661 ctgttttttt gtaggggcgg atatgatcac ttccactgca ggcttcagtt ttcgagccga
     3721 taccgaaacc ggcatatatt atccgggagc acgaattccg attgggaata gtgctacatt
     3781 aaagattcga attccggaaa aatctatctg ccgattaatt agcaacggaa aagtgtataa
     3841 acaatgggaa agtgttcaat cggttccgat aacaattaca gaacccggct attatcgagt
     3901 cgaatgttat ttaccctata aaaacaagtt gagaggatgg atttattcca atccgattta
     3961 ttttttcaaa ggataatttt ttcgatgact actacgaaca atgttattcg attcctttct
     4021 tcaaaaaaaa tcgattttca gatttttgaa tccgaaccga ttcggcgaag cgcgatggag
     4081 acagccgctc tctatcattt ggatcctatg attgtatata aaacaatcgt cgcatttaat
     4141 ccgatccgga aaaaatattt gctttgtatc attccggcac cctatgaagt aaatttaaaa
     4201 aaattagcgt cggcgattgg tgaaaaaaaa gcggccatcg tcacgcaaca tgaagctgaa
     4261 gttttaaccg gtcttgaagc cggagggatt tctccttttg cgttgtatca taaagggttt
     4321 caaatttggg ttcatgaaac tatcagggat caggaaaaaa tctcggtttc cggcggccag
     4381 cgcggattat ccatccttct atcaacgagt gatttcattc agattttgca accgaaaatt
     4441 gccgatatag ccgatattca acaagacata caaagttctg aatgataaaa acaacccgat
     4501 ata
//
LOCUS       DAOMIV010000110         4484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_19955_length_4484_cov_5.139761, whole genome shotgun sequence.
ACCESSION   DAOMIV010000110 DAOMIV010000000
VERSION     DAOMIV010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19955_length_4484_cov_5.139761"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PKV59_03730"
     CDS             <1..786
                     /locus_tag="PKV59_03730"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQP45894.1"
                     /translation="DEALQIRSENKVIPYAGGTDLMIKAEDVPYLFLNKLAELQQISV
                     DGDMIRIGAGCTYTSLLDSPITPKVLKEAIRLIAAPAIRNLGTVGGNICNASPKADSA
                     LIFYVSDSKLRLRSVKGERILPIEDFYVGRGKTVLQPDELLIEILMPKKYLDNYYYQK
                     VGARQALAISRVAFVGLFGMEDGKITCCSTAYGSVSDTILRFRNLEAMLLGKTIAEAK
                     ELKGSFLAEYDKAIVPIRGRVSADYRKRVCMNLLEDYLTQNGI"
     gene            1004..2032
                     /locus_tag="PKV59_03735"
     CDS             1004..2032
                     /locus_tag="PKV59_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HQP45895.1"
                     /translation="MDQFLPTGKIKPELLKDIIQRAPVFDHRIVLGPGIGLDCGVIDG
                     GDRYLVMKTDPITFATEEIGWYAVQISANDIATTGAKPEWMMITALLPEKSTTEQLIL
                     EITDQIYQAAAEINISLVNAHTEITVDLNRPILVCSLIGEAEKDRLVTPKGVRPGNEL
                     LLTKGIPIEGTALLAKEFPNLLKEVLSEDELIEAKAFTHIPGIGVTKDAQIALQNGTV
                     TGMHDPTEGGLASALWEFSTASGYALEINPERIPIPEISAKICKRFHLNPLNTIASGA
                     LLLSVEAQDSAKIIQALEKADIPCSKIGLVTDREEVSVSDIKNKNLLPWPEQDDITKV
                     FQHENRLF"
     gene            complement(2024..4351)
                     /locus_tag="PKV59_03740"
     CDS             complement(2024..4351)
                     /locus_tag="PKV59_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HQP45896.1"
                     /translation="MIPSTSNSFNSNVLFSIQIILAQYPILQDRISEAMFNRLVQDGY
                     TTFAEFEERVRDFALMSQRREGLQNPFGQEGPNIWENRLQRVRAQLINVEFSQHYSIN
                     VFNSIVNDVIQNRVVEKQPLFMWHNLEFASEETIFDQALAIERMPAAERKEYESKLTG
                     AKVVLIRRLLSEQLPYVEIAKNYFSIQDLIEISKHRIGKGCIGGKSAGMLLARRILQG
                     AEDEEIRSSATAKESFFIGADEYYSFLSINDRLDLLDFRYDNEDQYWSRYPEIRESFK
                     NTNFSKEIIESLERVVREFQGKPFIVRSSSLLEDGFNYSLSGLYESHVIANQGSVSEG
                     LNKLLDAIRDIYAGTFDPRVLTYRLRNGLIDYPESMGILIQVITGNKQGNYLFPDISG
                     VGASKSPFIWQGNSKNTSTDEGYIRVVCGLGTRAIKRSGYDFSQIVVLNDPDKIRTVF
                     SIDDPIYSQEMIDVINLKNNQFESLDVTSIVNEKYNLFPWMAQIYKDGSLQPVVRGMN
                     PDRYVINFNELIKKSSFTKLLRDILSILEKAYRRPVLIEFTILVNLSNGALPSFQVQI
                     DKCRPVNISDRMKGQWTFEEIDPNSKFLETDLYVQNGLLKNIDYVVWIDSHELAKFAG
                     NNPHTAAGLIRNLNKAMENQRFILMSNDRFGTSDMGGGLPVAYSDITNAQAIVEISDS
                     QKGKNADPMVGSQFYQNLLEAGIFTISINLERKGCFFDQAFLETQLNQLTSWIDQPAA
                     VKAVQVLPVKGYRGFEKLNIAFDQNRGKTTAYFSK"
BASE COUNT         1272 a          983 c          994 g         1235 t
ORIGIN      
        1 gatgaagcct tacaaatacg ttcggaaaat aaagttatcc cttatgccgg cggaacggat
       61 ttaatgatta aagcggagga cgttccttac ctttttttga ataaattagc ggagttgcaa
      121 cagatatcag tcgatggcga catgattcga atcggtgccg gctgtaccta tacatctcta
      181 ttggacagtc cgatcacccc gaaagttttg aaagaggcga ttcgtctgat cgcggcaccg
      241 gccatccgca atctcgggac ggtcggagga aatatttgca atgcgtcgcc aaaagccgac
      301 agcgccttga ttttttatgt gtcggattca aaattacgtc ttcgcagtgt caaaggcgaa
      361 agaattttgc cgatcgaaga tttttatgtt gggcgcggaa aaaccgtctt gcaacccgat
      421 gaattattaa tcgaaatctt aatgccgaaa aagtatttgg ataattacta ttatcaaaaa
      481 gtgggcgccc gccaggcgtt agccatctca cgagttgcct tcgtcggttt attcgggatg
      541 gaagacggga aaattacctg ttgttcgacc gcttatgggt cggtcagcga taccatcctg
      601 cgatttcgga atcttgaagc catgctgctg ggaaaaacga tcgcggaagc aaaagagctg
      661 aaaggttcat tccttgcgga atatgataaa gcgattgttc ccattcgcgg acgggtatcg
      721 gcggattatc ggaagcgtgt ctgtatgaat ttattggaag attatttgac acaaaacgga
      781 atctgatgct tggaagtgat cagcggggtt aataaagaac cggactcgat taagtccggt
      841 tctttatttg aaaattttat tttcaagttg aacagcgaaa ttttaaggga tttgctaaaa
      901 ggaatcgaaa ttcggtgacc ttcaggtttt tattcgtttc cggagagatt ctgttttcat
      961 aatgttatac ttgcctctgc gaataaactt agggagctgg attttggatc aatttttgcc
     1021 gaccggtaag atcaagccgg aattattaaa ggatattatt caacgggcgc cggtttttga
     1081 ccatcgtatc gtactcggac ccgggatcgg attggattgt ggcgtaatcg atggcggaga
     1141 ccgttacctg gttatgaaaa cagatccgat cactttcgca accgaagaaa tcggttggta
     1201 cgcggttcaa atcagcgcaa atgatatcgc aacgacgggt gcgaaacccg aatggatgat
     1261 gataacagcc ttgttgcccg agaagtcgac aaccgagcag cttattctcg aaattaccga
     1321 tcaaatctat caggctgccg cggaaatcaa tatttcatta gtgaatgctc atacggaaat
     1381 cacggttgat ttaaatcggc cgattcttgt ttgttccctg atcggcgagg cggagaagga
     1441 ccgtttggtt acaccgaaag gggtgcggcc ggggaatgaa ttactattaa caaaagggat
     1501 cccgattgaa gggacggctt tattggcaaa agaatttccg aatcttctga aagaagtatt
     1561 gagtgaagac gaactgatcg aggcgaaagc attcacccat attcccggaa tcggagtaac
     1621 caaagatgcg caaattgcgc ttcaaaacgg aaccgttacc ggtatgcacg atccgacgga
     1681 gggcggtttg gcttccgctt tgtgggaatt ctcgaccgct tcgggttatg cgttggaaat
     1741 caatcccgaa agaattccca ttccggaaat atccgcaaaa atatgcaagc gatttcattt
     1801 aaatccgttg aataccattg catccggcgc attgctgctg tcggtggaag cgcaggacag
     1861 cgcaaaaatt attcaagcgt tggaaaaagc cgacatcccc tgctcgaaaa tagggctcgt
     1921 cacagatcgg gaagaggttt ccgtctcgga tattaagaat aaaaacctgc tcccttggcc
     1981 ggagcaggat gatattacga aagtttttca gcatgaaaac cgtttatttt gaaaaataag
     2041 cggttgtttt ccctcgattt tgatcaaaag cgatattgag tttttcaaat ccccgatatc
     2101 ctttgacagg caagacctga accgctttta ccgctgcggg ttgatcaatc catgaagtca
     2161 attggttaag ttgtgtctcc aaaaaagcct gatcaaaaaa gcaacctttc cgttccaaat
     2221 tgatactgat cgtaaagatg cccgcttcca ataaattttg ataaaattgt gagccgacca
     2281 tcggatcggc gtttttgcct ttttgcgaat cggagatttc cacaattgcc tgagcattgg
     2341 taatatcgct ataagcgacc ggcaaaccac cccccatatc ggaagtgccg aaacgatcat
     2401 tgctcatcag aataaatcgc tgattttcca ttgccttatt caggtttctg atgaggccgg
     2461 cagctgtatg cgggttattt ccggcgaatt ttgccaattc atgagaatca atccaaacaa
     2521 cataatcaat attcttcaac aacccgtttt gaacatacaa atcggtttca aggaattttg
     2581 aattcggatc gatttcttcg aaagtccact gtcctttcat tcgatcggat atattcaccg
     2641 ggcggcattt atcgatctgc acctggaaag agggcaatgc cccgttcgac agattgacca
     2701 aaatcgtaaa ttcaataagg acaggccgtc tgtacgcctt ttcaaggatt gaaagaatat
     2761 cccgcagaag cttggtaaat gaacttttct tgatcaattc gttgaaatta atgacataac
     2821 ggtccggatt cattcctcgg acaaccggct gcaaggaacc gtctttataa atctgagcca
     2881 tccaagggaa caggttatat ttctcgttga caatacttgt tacatccaac gattcaaatt
     2941 gattgttttt caaattgatg acgtcgatca tttcctgaga ataaatcggg tcatcgatcg
     3001 agaaaaccgt gcggatttta tccggatcat tcagaacaac tatttgcgaa aaatcatatc
     3061 cgcttcgctt aatggcacgg gttcccaacc cacaaaccac tcgaatataa ccttcgtccg
     3121 tagatgtatt ctttgaattt ccctgccaaa taaacggact tttgcttgcg cccactcccg
     3181 agatatccgg gaaaagataa tttccctgct tatttccggt aattacctga atcaggatcc
     3241 ccatgctttc ggggtaatca atcaaaccgt tacgaagacg ataagtcagc acgcgcggat
     3301 caaaagtacc ggcataaata tcgcggatcg catctaacag tttatttaat ccctcggaaa
     3361 ccgatccctg attggcgatc acatggcttt catagagtcc gcttaaagaa taattgaacc
     3421 cgtcttccaa aaggctggaa gatcggacga taaacggttt cccttgaaat tcccgcacaa
     3481 cccgttcgag actttcgata atttcttttg aaaaattggt atttttaaag ctctcgcgta
     3541 tttccggata acgactccag tattggtctt cattgtcata acgaaaatca agcaaatcca
     3601 atcgatcgtt gatggaaaga aaagaataat attcatccgc accgatgaaa aacgactctt
     3661 ttgccgtagc ggaactgcga atttcctcat cctcggcgcc ttgtaaaatt cgacgggcta
     3721 acagcattcc ggctgacttt ccgccgatac atccttttcc gatccgatgc ttgctgatct
     3781 cgattaagtc ctgaatggaa aaataatttt tggcgatttc cacataaggc aactgttcac
     3841 tcaaaagacg gcgaatcagc actactttgg cacccgtcaa ctttgattca tattcttttc
     3901 gctcggctgc cggcatccgc tcgatggcta aagcctgatc gaaaatcgtt tcttccgaag
     3961 cgaattcgag gttgtgccac ataaaaagag gttgtttttc aacaacccga ttttggatga
     4021 cgtcattaac gatggaattg aatacattta tggagtaatg ctgagaaaac tcaacattaa
     4081 tcaactgagc gcggacacgt tgaaggcgat tctcccaaat attcggtcct tcctgcccga
     4141 acggattttg cagtccctct ctccgttggg acatcaaggc aaaatcacgg actctctcct
     4201 caaactcggc aaaagtagta tatccatcct gtacaagccg attaaacatg gcttcactaa
     4261 ttctgtcctg caatatcgga tattgcgcca gaataatttg aatcgaaaac aatacattcg
     4321 aattaaatga gttgctggtc gacggtatca tttctgtttg tcatttttat cccaaatgca
     4381 tcggcataat cacgtattgg aattcattct cattgtcgag agggacaatc aaccccggcg
     4441 aaacatttgt attggtgcgc agagaaacgt tgggtgtggt gatc
//
LOCUS       DAOMIV010000111         4483 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_19964_length_4483_cov_5.315041, whole genome shotgun sequence.
ACCESSION   DAOMIV010000111 DAOMIV010000000
VERSION     DAOMIV010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19964_length_4483_cov_5.315041"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PKV59_03745"
     CDS             complement(<1..272)
                     /locus_tag="PKV59_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45897.1"
                     /translation="MIKVLHVITDLNTGGAEKMLAQVIPVLKSGGIEQSVVCLRSTGP
                     VAKIIEENGICVTCLNMKPAVPVTALFGDLKKQLQTIVPILFILGF"
     gene            complement(438..923)
                     /locus_tag="PKV59_03750"
     CDS             complement(438..923)
                     /locus_tag="PKV59_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQP45898.1"
                     /translation="MGKTNRGKIVLSKIFTLSNKIQILGDRLETGITIKQFQLLEVLS
                     KNDPAPMTITELSEALGSSRQNIKKMALLLEKQNYLVLSKDDTDGRIVRVALTEEGKN
                     SLQQQAESDKEFINQLFDGFDDKLLKNMSKGFDKLSKNILLMEEKTNNPGVPKETIKP
                     F"
     gene            1269..1964
                     /locus_tag="PKV59_03755"
     CDS             1269..1964
                     /locus_tag="PKV59_03755"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQP45899.1"
                     /translation="MRDQEKILLASGSQRRRELLSWLTDRYEIIPSDIDESPLPGELP
                     QVYCQRIAEQKALAIPSRTETQNSFVLAADTIVYSQNRIIGKPKDEQDAFQILTFLQG
                     QIHHVCTAVTLYRNGKTVSTFCDTSVRMNPLSQATIRSYIATGDPMGKAGAYAIQNTD
                     FNLVESVEGCYTCVIGLPLCHVEKLFSFFDYSFEKNVIECCKSHIHYNCVVDQSEMLR
                     KSLFNQKIIERNV"
     gene            1961..4222
                     /locus_tag="PKV59_03760"
     CDS             1961..4222
                     /locus_tag="PKV59_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQP45900.1"
                     /translation="MKRFIICFIFLCVFTAGCGPKVSDESTKTPEITTVMPSDTPLPE
                     PVVMITSAPEARTTANTFLEFWKADDFVSMYNMLSQVSRDAIPFEDFETKYKEAETNL
                     TLQSLNCEVTSALTNPKTAQIGYHATYQTALAGTFSRDMVMNLVFEANDWKIQWDDGM
                     IMPELSGGNKIEIDIDVPTRGPIYDIDGVPLAAETEAYAIGVVPASVPSNRWNGLINE
                     LSRLTGKTTFVITQLMEEANQYDYVVIGEVPAAVVEERMEAISSYEGVYLNSYTASRF
                     YYEGGSAPHLVGYVQPIGEDEAETMKRQGYRIDERIGRIGIEKWGQDRLDGERGASVY
                     VVDSAGNPITRLYKSDPVPAETIYTTFKEDFQYDLQRAIAGFRGAIVVLERDTGRVLG
                     MASSPTFDPNLLDFNNYNSFYSANLLYNDDRPMYNRATQGQYPLGSVFKIITMSAALE
                     SGVYHVNDTYDCGYEFTELPGITLTDWTLDHDVAASGKLTLSEGLMRSCNPWFYKIGL
                     ELFRQKGADYLPSMARGFGLGSPTGIGEVEEESGNVETPPDEYSSVQMGIGQGTLLVT
                     PLQVARFVAAVGNGGTLYRPQVVEQIVDYEGNVTYAFKPEEQGKLPVSKENLELVQQA
                     MLSVTNNPRGTAYSTFKYIKYPVYGKTGTAQTSPGEDPHAWFAGYTDRKLEGKPDIAV
                     AVLVENMGDGSVYAAPIFRRVLELYFDGKASMLYNWEAGVYITKTPTPTPTITPTPEP
                     TKAPWQITPEVTP"
     gene            4235..>4483
                     /locus_tag="PKV59_03765"
     CDS             4235..>4483
                     /locus_tag="PKV59_03765"
                     /inference="COORDINATES: protein motif:HMM:NF028055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45901.1"
                     /translation="MTLQGRVIRYQSGFYTVMTDERIVTCKIRGKLKENFRQTDLVAV
                     GDLVEFTQLQDGTGVIECILPRKNELIRMISGIKSEYRQ"
BASE COUNT         1271 a          956 c          964 g         1292 t
ORIGIN      
        1 ataaagccaa gaatgaataa gatcgggacg atcgtctgca gctgtttttt taagtcgccg
       61 aaaagcgctg taactggaac agccggtttc atgttgagac aagtcacaca aatgccgttt
      121 tcttcgataa ttttggcaac cggaccggta cttctcaaac acacaacaga ttgttcgata
      181 ccgccggatt tcagaaccgg aatcacttgt gcgagcattt tttctgcacc gccggtattt
      241 aagtcggtta taacgtgcaa tactttaatc attctgattt atattgtaaa ggaaattaac
      301 cggcgagcgg aatatccgaa taaattggtt ggcatggata actatttttt tacaaatgat
      361 cgacgaatcg cccgatttaa attcattttc ctgatttcat aacgaatttt tcaacccgat
      421 aacaaacgga agtcaattta aaaaggtttt atggtttctt tgggaacacc cggattattt
      481 gttttttctt ccatcaaaag aatattttta gataatttgt caaatccttt cgacatgttt
      541 ttcagcaact tgtcatcaaa tccatcaaaa agttggttga taaattcttt gtccgattcc
      601 gcttgttgtt gtagactgtt tttcccttct tccgtcaagg caactctgac aattctgcca
      661 tccgtatcat ctttgcttaa aaccaaataa ttttgtttct caaggagtaa agccatcttt
      721 tttatatttt gtcgggaact tcccagtgct tccgacaatt ccgtaatcgt cataggagcc
      781 ggatcatttt ttgaaagcac ttccagcaat tggaattgtt taatcgttat tccggtttcc
      841 agtcgatcac ctaatatttg aattttattg gaaagcgtga atattttaga caatacgatt
      901 ttgccgcgat tcgttttacc catatgtcgc cctcctgtca aaagggaacc tattatcttt
      961 ataaaacaag tatacctaat gaagcgcccc gtaacaatac attaattttt tacgcctctt
     1021 gcttttattc cgtttttcat cttcatgtgc aatttcaaat aaggtataag gtcccacaaa
     1081 tcaaatagat tgaccgggcc ttcataatca aatcaactcc ataaaaatcg attcaccggt
     1141 ttatcctgtt tttaccgatt cccacataga tacctcattt tgtcatcgat ctttaaatac
     1201 gacatccttt cttgactacc cgttggcatt catttatcgg aacattcaat tgtgttagaa
     1261 ttttccttat gcgagatcaa gaaaaaattt tgttggcgtc cggctcccaa cgcagaagag
     1321 agttgctctc atggttaacc gatcgttatg aaattattcc ctctgatatt gatgaaagtc
     1381 cgctgcccgg agaacttccg caggtgtatt gtcaaagaat cgcggaacag aaagcgcttg
     1441 ctattccttc cagaacggaa acgcagaatt cttttgttct tgcagccgac accattgttt
     1501 attcacaaaa tcggatcatc gggaaaccca aagatgagca ggatgcattt caaatactga
     1561 cctttttaca aggacaaatt catcatgtct gtactgccgt aacattatat cggaacggga
     1621 aaactgtcag tactttctgc gacacatcag tccgtatgaa tcccttatcc caagccacta
     1681 tccgatctta catcgcaacc ggtgacccta tgggtaaagc gggtgcttac gcaatccaaa
     1741 ataccgattt taatttggtg gaatccgttg aaggatgtta cacatgcgtt atcggtctcc
     1801 ctttatgcca tgtggaaaaa ttgttttcat tctttgacta ttcctttgag aagaatgtta
     1861 tcgaatgttg caaaagccat attcactaca attgtgtagt ggatcagtct gaaatgttac
     1921 ggaaatcctt attcaatcaa aaaattatcg aacgaaacgt atgaaaagat tcatcatttg
     1981 ctttattttt ctttgtgttt ttaccgccgg atgcggtccg aaagtttccg acgaatcgac
     2041 aaaaacgcct gagataacca cggtgatgcc gagtgataca cctctgccgg aaccggtggt
     2101 tatgataacc tccgctccgg aagcgagaac aactgcgaat acattccttg aattttggaa
     2161 agcggatgat ttcgtcagta tgtataacat gctttcccaa gtcagcagag atgctattcc
     2221 gttcgaagat tttgaaacca aatataaaga agctgaaacc aacttgactt tacaaagttt
     2281 aaattgtgag gtgacctccg ctttaactaa ccctaaaacc gcgcaaatcg gatatcatgc
     2341 gacctatcaa accgcgttag cgggaacttt ctcgcgcgat atggtaatga atttggtttt
     2401 tgaagcgaat gattggaaaa tacagtggga tgacgggatg atcatgcccg agctgagcgg
     2461 tggaaacaaa atcgaaatag atatcgatgt accgacgcga ggaccgattt atgacatcga
     2521 cggtgttccg cttgccgcag aaacagaagc atatgcaatc ggggttgtgc ctgcttccgt
     2581 cccatcgaac cgttggaatg gcttaattaa tgaactttca cgactgaccg gtaaaacgac
     2641 ttttgtgatc acacagctga tggaagaagc taatcagtat gattacgtgg tcatcggtga
     2701 agtccccgca gcggttgttg aagaacgaat ggaagccatt tccagttatg aaggtgttta
     2761 tctcaattct tatacagcaa gccgttttta ctatgagggc ggatctgctc cgcatttggt
     2821 cggttacgtt caacccatcg gcgaggatga agccgaaacc atgaaaagac aaggatatcg
     2881 gattgatgaa cgaatcggaa gaatcgggat cgaaaaatgg ggtcaggatc gtttggatgg
     2941 cgaacgagga gcttctgttt atgtcgttga ttcagccggg aacccgatca cccgtttata
     3001 taaaagcgat cccgtccctg ccgaaacgat ctatacaacc ttcaaagaag atttccaata
     3061 tgaccttcaa agggcgatcg ccggatttag aggcgcaatt gtcgtgttgg agagggatac
     3121 cggcagagtt ttaggaatgg cttcttcacc gacttttgac ccgaacttgt tggattttaa
     3181 taattacaat tctttttaca gtgcaaattt gctttataac gatgaccgtc ctatgtacaa
     3241 ccgtgctacc cagggacaat atcctttggg gtccgtgttt aaaataatca ccatgtcagc
     3301 cgctttggaa tccggcgtat atcacgtcaa tgacacttac gattgcggtt atgaatttac
     3361 ggaattgccc ggaatcactt taaccgactg gacactcgat catgatgtcg cggcaagcgg
     3421 taaactcacg ctttcggaag gtttgatgcg ctcctgtaat ccttggtttt ataaaatcgg
     3481 tctggagctt tttcggcaaa aaggtgcgga ttatttaccc agtatggcaa ggggattcgg
     3541 cctcgggtcg cctaccggca tcggtgaggt ggaagaggaa tccgggaatg tcgaaacacc
     3601 tcccgatgaa tattcatccg ttcaaatggg tatcggacaa gggactttat tggtcacacc
     3661 gcttcaagtc gcccgttttg tggcagccgt cggtaacgga gggacactat atcgtcctca
     3721 ggttgtagaa cagattgttg attacgaagg aaatgttact tacgctttta aacccgaaga
     3781 acaaggcaaa ttaccggttt caaaggaaaa tttggaatta gtccagcaag caatgctgag
     3841 tgtgacgaat aatccgcgcg gtaccgccta ttcgactttt aaatatatta aatatccggt
     3901 ttacgggaaa accggtacgg ctcagacatc acccggagaa gatccgcatg cttggtttgc
     3961 cggatatacc gatcgaaaac tggaaggaaa accggatatc gcggtggccg ttctggtgga
     4021 gaacatgggg gatggttcgg tttatgcggc tccgattttc cgcagagttc ttgaacttta
     4081 ttttgacgga aaagcatcca tgctttacaa ctgggaagcg ggggtttata taacgaaaac
     4141 accgactcct acgccaacga tcactccgac accggaaccg acgaaagcgc cttggcaaat
     4201 aacacctgag gttacccctt aaatcgaccc gcgaatgact ctgcaaggac gcgtgattcg
     4261 ttatcaatcc ggattttata ccgtcatgac cgatgagagg atcgtgactt gtaaaatacg
     4321 cggtaaatta aaggaaaact tccgtcaaac agatttggtt gccgtcggtg atttagtcga
     4381 gtttacgcag cttcaagatg gaacaggtgt tatcgaatgt atccttccac ggaaaaatga
     4441 actaattcga atgatttccg gtatcaaatc ggaatataga caa
//
LOCUS       DAOMIV010000112         4474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20016_length_4474_cov_4.105454, whole genome shotgun sequence.
ACCESSION   DAOMIV010000112 DAOMIV010000000
VERSION     DAOMIV010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20016_length_4474_cov_4.105454"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..1703)
                     /locus_tag="PKV59_03770"
     CDS             complement(48..1703)
                     /locus_tag="PKV59_03770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HQP45902.1"
                     /translation="MKQEELQFIPLGGLGEVGRNMMVFGYEDRYIIIDCGVQFPDNSM
                     IGVDYIIPDMQFLQGKEDKILGIIITHAHEDHIGGLPHLFDVLNNVPIYATPLTAGMI
                     EVRLARAGKLTKTKINVKKAGEEFDLGKFHIETFHMTHSIPDSIGLAITTPAGLIVVS
                     GDYKFDPTPIDNWPSDFAKLAELGTRNVLALFSDSTNSERPGTTPSEKDINKAFEEVF
                     VNARGRVIISSFASLISRLQQLSTIARRHHRKICIVGTSMVDNVNLAKRLGYLDLPDE
                     WVVTLEEALHLPDKEVLILCTGSQGEPTSIMGRLSTGKYYAFSIKEGDTVMLSSNPIP
                     GNEERVSAVINRLYRMGANVIYNSLLSVHVSGHACQEEMKLLINLVKPEYFIPVHGEL
                     RHLYNQAKIGELMGIPKNRIFVVENGQTIKFHHGKARLAEQVPASMVLVDGTNVGDMN
                     LDIMHQRERLADSGVIVITLMLDKDHHGLIRDPQIIHYGLMSGKETPDIEEDLRRTII
                     EYCGKKNSTGTNNIEKNIEQIVRKFIYNTTHRSPWVIVNPFYF"
     gene            complement(1707..2639)
                     /locus_tag="PKV59_03775"
     CDS             complement(1707..2639)
                     /locus_tag="PKV59_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQP45903.1"
                     /translation="MEYKDYYKILGVDKNASNEAIRKAYRDLAKKYHPDHNPGNKAAE
                     EKFKEINEAYEVLKDKDKRARYDQLGSSYSAWQQQGGNSANYNWSDWFSTAPGGQGTY
                     YATDFENMGDFSDFFSQIFGGMGSSRRSRTSRSTGFGQSYTPTKPAPVEYTLPITLKE
                     AFTGTERILTIGNKRIEAKIPAGAKTGTKVRLAGVMGEESGNADLILNIKVEDDPQFE
                     RVNDDLYVDVPIDLYTAILGGEVIVPSINEKIVLKIPAGTQPGQLIRMAGKGMPKLKG
                     KGTRGDLYARIKVQIPRNLTEKQKKLFEELRKEK"
     gene            complement(2945..3826)
                     /locus_tag="PKV59_03780"
     CDS             complement(2945..3826)
                     /locus_tag="PKV59_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQP45904.1"
                     /translation="MIFEKVSEHIWYMPREDETDRPALGYVNGGKTSLMVDSGNSPEH
                     ISYFYGVVRSMGMPDPGFVVLTHWHWDHVFGLTGSPAKSVAHVLTNERLQIMQRWDWS
                     DSSISARVSAGVETQFAFDMIRKEMPSRHSFKVKQADISFEVQCAIRLEDLECQLVHV
                     GGPHSDDSIVVYVPQEKVLFLGDCAYPDIYNNNSLRLSELSSLILRLEQFEADLFIPS
                     HSDPKSKNQFMSELKSLEKIGIAVGDLTDPALLPALLEKELGHQPTKEEIESAEHFVI
                     GNQLSNRNLMDHPFFFK"
     gene            complement(4049..>4474)
                     /locus_tag="PKV59_03785"
     CDS             complement(4049..>4474)
                     /locus_tag="PKV59_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45905.1"
                     /translation="EPLEDPLLLDDDESEADLSNVLEEGEGVNSKSIAYNACGKQRIE
                     INFNKPDIYNITWYVTNAGSAVWNSDKYRVVNSGLSDNLVMMGTDITVPATNPGEEAM
                     VSMSVKVKDVADQSDRWLKYYLTDGNESFCEVYFSLPIF"
BASE COUNT         1183 a         1016 c          886 g         1389 t
ORIGIN      
        1 catttgcatc cattgacaga aaaaaacgga ttgtttctgt gtatatttca gaaataaaac
       61 gggtttacga tcacccaggg cgagcgatgt gtcgtattgt aaataaattt ccgaacaatt
      121 tgctcgatat ttttttcgat attattcgtt ccggtcgaat tctttttacc gcaatattcg
      181 ataattgttc ttcgcagatc ttcttcgata tcgggagttt cttttccgga cattaagccg
      241 taatggataa tttgcggatc gcgaattaaa ccgtggtgat ctttatccaa cattaaggtt
      301 attacgatga cgccgctgtc agccaagcgt tcccgctggt gcatgatatc gagattcata
      361 tctccgacgt tcgttccatc caccaaaacc atggaggcgg gaacctgctc ggctaatcga
      421 gcttttccgt gatgaaactt tatggtttgc ccattttcca cgacaaagat gcggtttttc
      481 ggaattccca tcagttcacc gatttttgcc tgattataaa gatggcgaag ttcaccgtga
      541 accggtatga aatattccgg tttcactaaa tttatcagca atttcatttc ttcctggcag
      601 gcatgtccgg agacatggac ggagagaagc gaattgtaga tgacatttgc tcccattcga
      661 tacaaccggt taattacagc cgaaaccctt tcttcgtttc cggggatcgg attggaggaa
      721 agcattacgg tatcgccttc tttgatgctg aaagcgtagt actttccggt ggagagtctt
      781 cccataatag aggtgggttc gccctgagaa ccggtgcaaa ggatcagtac ctctttgtca
      841 ggtaaatgta gagcttcttc gagagttacg acccattcgt ccggcaaatc caagtagcct
      901 aacctttttg ccaagttgac attatccacc atactggttc cgacaataca aattttccga
      961 tggtgtcgcc tggcaattgt ggaaagttgt tgcaagcggg aaattaatga tgcaaaactg
     1021 gaaataatga cccgacctct tgcgttgaca aaaacttctt cgaaagcttt attgatatct
     1081 ttttccgaag gggttgttcc gggccgttca gagttggtag aatcgctaaa taaagctaat
     1141 acatttcgtg ttcccaactc ggctaatttc gcgaaatccg aaggccaatt atcaatcggt
     1201 gtcggatcga atttatagtc cccgctgacg acaatcagac ccgccggagt ggtaatggcc
     1261 aaaccgattg aatccggaat cgaatgcgtc atatgaaaag tttcgatatg aaactttccc
     1321 aaatcaaact cttctccggc ttttttaacg ttaatttttg tttttgtcag ttttccggca
     1381 cgggctaacc tgacttcgat cattcccgct gtcaacgggg ttgcataaat cggaacatta
     1441 ttcaatacat cgaataaatg cggtaatccg ccgatatgat cctcatgagc atgagtgata
     1501 ataattccga ggattttgtc ttcttttcct tgcagaaatt gcatatccgg aatgatgtaa
     1561 tctactccga tcatggaatt atccggaaac tgcacgccgc aatcgatgat gatatatcgg
     1621 tcttcatagc cgaataccat catattccgc ccgacttcac ctaatccgcc gagcggaata
     1681 aattgcagtt cttcttgctt cattctttat ttctcttttc ttaattcttc aaataacttt
     1741 ttttgtttct cagtcaagtt gcgcgggatt tgcactttga tacgagcata taaatcaccg
     1801 cgcgttcctt tgccttttaa ctttggcatt ccttttcccg ccatccggat taattgaccg
     1861 ggttgggttc cggcgggaat tttcaatacg attttttcat tgatggatgg aacaatcact
     1921 tctccaccca gaattgcggt atataaatcg atcggcacat caacgtacaa atcatcatta
     1981 acacgctcga actgcgggtc atcttccact ttgatgttca gtatcagatc cgcatttcct
     2041 gattcttcac ccattactcc ggccagtctg acttttgtcc cggtttttgc accggccggt
     2101 attttcgctt caatgcgttt gttcccgatc gtcaggatac gttccgtacc ggtaaaagct
     2161 tcttttaagg taatcggaag cgtatattct accggagcgg gtttggtcgg agtgtaactt
     2221 tggccgaatc ccgttgaccg agaggtcctt gaccggcggc tggagcccat tccaccgaag
     2281 atttgggaga agaagtcgga gaaatcgccc atattctcaa aatcggtagc ataataagtc
     2341 ccttgtccgc ccggtgcggt tgaaaaccaa tcgctccaat tgtaattcgc actattgcca
     2401 ccctgttgct gccaagcgct gtaagacgag cccaattggt cataacgcgc tcttttgtct
     2461 ttatctttta aaacttcgta agcttcatta atctctttaa atttctcttc cgcagctttg
     2521 tttcccggat tatgatcggg atgatatttc ttagccaaat cccgataggc tttgcgaatt
     2581 gcttcattcg acgcattttt atctacgccg agtattttat aataatcttt atattccata
     2641 ctcctccact ttctgtttca ttcctatccc aatcattcaa atcggtttgt caatagaaac
     2701 aggtctttaa gcagagattt ttttacacat atctccattt ctaccatatt ttacaatcgg
     2761 attatttgat ttttataaat tatatggaaa tcaaaataca aacagaggtg ttattcttca
     2821 aaatttcaga atgttcgccg atcatttcat gcggattcga ttaaaaacaa ttacggaaaa
     2881 ccgatttatt atgggtttta atcgaaaagc aacctttttt gcgatgaatt tttgcttctt
     2941 ggccttattt aaagaaaaac ggatgatcca tcaaattccg attggaaagt tgattcccga
     3001 taacaaaatg ttctgccgac tcgatttctt cttttgtcgg ctgatgtccc aattcttttt
     3061 cgagtaaagc aggcagaagg gcgggatctg tcagatcacc gactgcaatg ccgatctttt
     3121 ccagtgactt caattcggac ataaattgat ttttactctt cgggtcactg tgggaaggaa
     3181 taaacaagtc agcttcgaat tgttctaatc gtaagattaa ggaacttaat tcggacaagc
     3241 gcagactgtt attgttataa atatccggat atgcacagtc acccaaaaac aggacttttt
     3301 cctggggaac ataaacaacg atcgaatcat cgctgtgggg tcctcccaca tgtaccaact
     3361 ggcattcgag atcttccaat cgaatcgcac attgaacttc aaaagaaata tccgcctgtt
     3421 tgactttaaa agaatgtctt gaaggcattt cctttcgaat catgtcaaag gcaaattgtg
     3481 tttcaacgcc tgccgacaca cgggcactga tggagctgtc tgaccaatcc cagcgttgca
     3541 tgatttgcaa tctttcattt gtcaggacat gggcgacgga tttggctgga gatcccgtca
     3601 atccaaaaac atggtcccaa tgccagtgag tcagtacaac aaatccggga tcgggcattc
     3661 ccatgcttcg tacaacgccg taaaaataag agatatgttc cggtgaattt ccggaatcaa
     3721 ccattaaact ggtcttaccc ccattcacat acccgagggc cggccggtct gtctcatctt
     3781 ctctcggcat gtaccaaata tgttcactga ctttctcaaa tatcattttt ccctacctaa
     3841 tcctgttcta atggcaaatc caattttagc ttatccctga aaaactgaca aatgaagaaa
     3901 attttgtctt cgaatattct ataaataatt tccaaaattc gatttattct gattgtttcc
     3961 acaaagaaat ggattgataa aaaatctctt taaactgcaa aaaccccgaa gaaaggggtt
     4021 ctgacgggga ttttgcagtt tctgggtttt agaaaatagg aagggagaaa tagacctcgc
     4081 agaatgattc gtttccgtct gtcaggtaat attttagcca tcgatccgat tggtcagcta
     4141 catcttttac tttaaccgac atcgatacca ttgcctcttc tcctggattt gttgccggga
     4201 cggtgatatc cgtccccatc ataacgaggt tatcgctcaa acccgaattc actacccgat
     4261 atttatccga gttccaaacc gcagaccccg cgttggtgac ataccaagta atattgtaga
     4321 tatccggctt gttgaaattg atctcgattc gctgcttgcc acaggcattg tacgcgatgg
     4381 atttcgaatt tactccttct ccttcttcga gtacgttcga taaatccgct tcggattcat
     4441 cgtcatctaa aagaagagga tcttccaaag gttc
//
LOCUS       DAOMIV010000113         4436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20264_length_4436_cov_4.000457, whole genome shotgun sequence.
ACCESSION   DAOMIV010000113 DAOMIV010000000
VERSION     DAOMIV010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20264_length_4436_cov_4.000457"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKV59_03790"
     CDS             <1..203
                     /locus_tag="PKV59_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45906.1"
                     /translation="SNFRNDRRHYALVDILFPNKMNALFTRNIGVFRAFLFFPNCKTR
                     EKETDHESFQKQIMIRYFICGK"
     gene            complement(206..415)
                     /locus_tag="PKV59_03795"
     CDS             complement(206..415)
                     /locus_tag="PKV59_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HQP45907.1"
                     /translation="MNRLGKFITGAAIGGLIGIGAILLFAPDKRDEWFDEIQEKTQDI
                     LVEFNRAAQEYKEEMEEDLKARKGA"
     gene            complement(576..1595)
                     /locus_tag="PKV59_03800"
     CDS             complement(576..1595)
                     /locus_tag="PKV59_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQP45908.1"
                     /translation="MPISPYLPIVVVGTLRRDFVIPPGGKPLNDIPGGHLLHTAVHLH
                     HWENRIGLVSRIGPDFPQEWLDHLTELGMDVHGIKRTALPIEHRFFIRYDGNYQKSTL
                     YPLAQYANEGLPFPKELLGYNVQTNKIDSMNAKTDETIIARDIPENYLESTFIHLCPM
                     DYMTHNLLTQHFAQFGPKRITLRAGKGYMVPQFWHAIPLLVNSLTAFITSISGLKSLF
                     SETRETDIWKMAKIISEWGPEYVIIEQDNFSNLLWDRNHQARYRLGPYQNKVVNLIGI
                     HDAFCGGFIGELKKTYDPVKALIAGNVASSIAMEANHPFYALDTIPGLHEARADYLYS
                     KLELL"
     gene            complement(1598..1786)
                     /locus_tag="PKV59_03805"
     CDS             complement(1598..1786)
                     /locus_tag="PKV59_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45909.1"
                     /translation="MSDKNWKRNTLLIGAGIGLLTGLLSAYLRIRKAESNNEIITFKA
                     KDTAKIGTAVFDFMKKFL"
     gene            complement(1793..2215)
                     /locus_tag="PKV59_03810"
     CDS             complement(1793..2215)
                     /locus_tag="PKV59_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45910.1"
                     /translation="MDQSDQTNRKQEQPRQKKHSLVLATVVIFIAFAVIASLMIYFNR
                     DHPAGVIKTANYLVAIIFFILFVISIGLTFIIVKVIQRVDYWLGTLPQFTKQANLQIE
                     SLRSKVAGMTDDITEPIITAEAKFRALHDLFKTKKPRS"
     gene            complement(2434..3699)
                     /locus_tag="PKV59_03815"
     CDS             complement(2434..3699)
                     /locus_tag="PKV59_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HQP45911.1"
                     /translation="MANITKEDALTYHQLNGVPGKISVVPSKPLETFIDLSLAYTPGV
                     AFPVLEIAEKPETVYDYTDKGNLVAVISNGTAILGLGNRGSLASKPVMEGKGVLFKKF
                     ADIDVFDIEVDTTDKERFIDTVAAIGNTFGGINLEDIKAPECFEIEQRLQQMLSIPVF
                     HDDQHGTAIILGAALLNALEIVNKKIERVKIVINGAGAAGISCARHLIRLGVKRENLF
                     LCDSTGLVYKGRQDHMNPQKEEFANGDHPAKLDEVIRDADVFCGLSTADVLTAEMIKT
                     MAKDPCIFGMANPNPEIKYEIAREARPDAIIATGRSDYPNQINNVLGFPYIFRGALDV
                     RASKINEEMKLAASYALAELAHLPVPDYVLKAYEIPSLEFGRDYIVPKAFDKRAKWKV
                     SAAIAEAAMKSGVAGIQIDIEAYQKRLGK"
     gene            complement(3825..>4436)
                     /locus_tag="PKV59_03820"
     CDS             complement(3825..>4436)
                     /locus_tag="PKV59_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45912.1"
                     /translation="NLKWLILSALFLGMTAASKIIYCVIAFAILAAAIESGVRQRNIW
                     KELFGSLVLFGIIALGSFFIFNPSVWYDPIGRISMMIGFHENYQVQESDIYPWWQPIV
                     WVTRSVAHHSDRFAPKSPLGKSPEHFFFSADELIFILACIGFFKIPREYRIYFYWFIF
                     GLLFLFIWGTKWVHYACVVTAPLCIAAYFGSKKVSVWLNRINP"
BASE COUNT         1292 a          965 c          885 g         1294 t
ORIGIN      
        1 attccaactt tcggaacgat cggagacatt atgcgctggt agatatactt ttcccgaata
       61 aaatgaacgc tctttttacc cgtaatatcg gtgtatttag agcgttttta ttttttccca
      121 actgtaaaac tcgggaaaaa gaaacggacc atgaatcgtt tcaaaaacag atcatgatcc
      181 gttattttat ctgcggaaaa taatattaag cacctttacg ggctttcaag tcttcttcca
      241 tttcttcttt atattcttgt gccgcccgat taaattcgac aaggatatct tgggtttttt
      301 cctgaatttc atcaaaccat tcatctctct tgtcaggagc aaaaagcaaa atcgccccga
      361 tgccaattaa tccgccgatc gcggctccgg taataaattt ccctaaacga ttcattttgt
      421 tctccttctg ccaatggtat tggttatttt aatgcaaatt atgcaaaagt gttcatccac
      481 ctattcatca agctaataaa gatttcattt tcggatctat ggaaaaatca ttctgccggt
      541 ttcaattgag aaaccgataa taaatttgaa aacctttata gtaattcaag tttactatat
      601 aaataatcag cccgtgcttc atgtaatccc gggatcgtat ccaatgcata aaaaggatga
      661 ttggcttcca tagcgatgga agatgcgaca ttgccggcga tcaatgcttt gaccggatca
      721 taggtttttt tcagttcgcc gataaatcct ccgcaaaatg catcatgaat gccgatcaga
      781 ttaacaactt tgttttgata aggtcccaat cgatatcgtg cctgatgatt tctatcccac
      841 agcaagtttg agaaattatc ttgctcgatt atgacatact cgggtcccca ttcggaaatg
      901 atttttgcca ttttccagat atccgtttct cttgtctcgg aaaacaagct tttcagaccg
      961 gaaatcgagg taataaaagc ggtcaagctg ttgaccaata acggaatcgc atgccaaaat
     1021 tgcggcacca tatacccttt tccggctctt aaagttatcc gtttaggacc gaactgcgca
     1081 aagtgttgtg tcagcaagtt gtgcgtcata taatccatcg gacaaagatg aataaaagtg
     1141 gattccagat aattttcggg aatatctctg gcgatgatgg tttcatcggt tttagcgttc
     1201 atactgtcga ttttattcgt ttgaacgtta taacctaata actctttcgg aaagggcaaa
     1261 ccttcgtttg catactgagc caaaggataa agggtggatt tctggtaatt tccgtcatat
     1321 cggataaaaa aacgatgttc aatcggtaaa gcggtccgtt taatcccgtg cacatccatg
     1381 cctaattcgg tcaaatgatc caaccattcc tgcgggaaat cgggtccgat gcgagaaact
     1441 aagccgattc gattttccca gtgatgcaaa tggacggcgg tatgcaacag atgcccaccc
     1501 ggaatatcat tgagaggctt gccacccgga ggaattacaa aatctcttct taatgtcccg
     1561 actactacga tgggtagata aggggagatg ggcattctca gaggaatttt ttcataaaat
     1621 caaatacagc cgtgccgatt tttgccgtat cttttgcttt aaatgttatg atttcgttgt
     1681 tcgattccgc ttttcgaatt cgaagatacg ccgaaagcaa accagtcagt aaaccgatgc
     1741 cggcacctat tagcagcgtg ttgcgtttcc aatttttatc actcataatg atctaactcc
     1801 gaggtttttt ggttttaaaa aggtcatgaa gagctctgaa ctttgcttcc gctgtaatga
     1861 tcggctctgt gatatcatcc gtcatacctg ccacttttga tctcaatgat tcgatctgta
     1921 aattcgcttg tttggtgaac tgcggcaatg ttccgagcca atagtcgacc cgctgaatca
     1981 ctttaacgat tataaaagtc aatccgatgc tgatcacaaa caagatgaag aaaataatcg
     2041 ctacaagata gttcgctgtt ttgattactc ccgcggggtg atcacggttg aaatatatca
     2101 ttaaagaggc tatgactgcg aaagcgataa aaataacaac tgtcgcaagg acaagggaat
     2161 gctttttttg tctcggctgt tcttgtttcc gattcgtctg atcagattgg tccatggtac
     2221 tccgtatttc tcaattatac tgaaaaacac cagcctgatt aaaaacttat ttgcgggata
     2281 gcttaaaaaa cctgtcgaat tgttttttat cggatgggga tcatgatgag aactgcaatc
     2341 ttttaaaaag aatcgcatcc ccgatccaat gatccgtcag aggatgcgat tcttctttgg
     2401 gaaagaattc cgttcagttt ttagagaata aaatcattta ccgagtctct tttgataagc
     2461 ctcaatatca atttgaattc cggcgacacc ggatttcatt gccgcttcgg caatagccgc
     2521 tgaaaccttc catttcgccc gtttatcaaa agccttggga acaatataat cgcggccgaa
     2581 ctccaaagat ggaatctcat aagctttcaa gacataatcc ggcaccggaa gatgagccag
     2641 ttctgccaac gcataggagg cggctaattt catttcttcg ttgatcttgg aagcgcggac
     2701 atccagtgca ccccgaaaaa tataagggaa acccagtaca ttattaattt gattgggata
     2761 atcggaacga ccggtggcga taatcgcatc cggtctggct tcccgagcta tctcatattt
     2821 tatttccgga ttgggattcg ccatcccaaa aatacaagga tcttttgcca tggttttaat
     2881 catttcagcc gttaaaacat ccgcggtaga aaggccgcaa aacacatcgg catctcgaat
     2941 cacttcatca agtttcgcag gatgatcacc gttagcgaat tcttcttttt gcggattcat
     3001 atgatcttgc cggcctttat aaaccaaacc cgtcgaatca cataagaaaa gattttcgcg
     3061 tttgacaccc aaacggatta aatggcgggc gcaggaaatt ccggctgccc cggcaccgtt
     3121 tatgacgatt ttaacccttt cgatcttttt gttgacgatt tccaacgcat tcaataaagc
     3181 agcgcccaga atgatggccg tgccgtgttg gtcgtcatgg aaaaccggaa tactcagcat
     3241 ttgctggaga cgttgttcga tttcaaaaca ttccggtgct ttgatatctt ccagattgat
     3301 tcccccaaaa gtatttccga tggctgctac cgtatcaata aaccgttctt tatccgttgt
     3361 atcgacttcg atgtcaaaga catctatatc ggcgaatttc ttgaacagaa cacccttgcc
     3421 ttccatcaca ggtttggaag ctaaggatcc gcgatttccc agccctaaaa tagcggtacc
     3481 gtttgaaatt acagctacga gattcccttt atcggtgtag tcataaaccg tttccggttt
     3541 ttcggcgatt tcaagcaccg gaaaagcaac tcctggggta taagcaagac tcaaatcgat
     3601 aaatgtctca agcggttttg atggaacaac cgatattttg ccggggactc cgttcagttg
     3661 atgataagtc aaagcgtctt cttttgtgat atttgccata aggttacctc aattgtcaat
     3721 agtaatgaaa aagatatcga tcattttatc atgtaaacgt ttacaaacca ataactttca
     3781 ttttcaaata cgaaatcgtc acattattct ctcgcattcg atcgttacgg attaattcgg
     3841 ttcagccaaa ccgaaacctt ctttgacccg aaataagctg cgatgcatag cggtgcagta
     3901 acgacacaag cataatgaac ccatttggtt ccccagataa aaaggaaaag aagcccgaaa
     3961 atgaaccaat aaaagtaaat ccgatattct ctcggaatct tgaaaaatcc gatacatgcc
     4021 aggatgaaaa tcagttcgtc agcggagaaa aagaaatgtt cgggactttt acccaaggga
     4081 cttttcggtg cgaatcgatc tgaatgatga gcgacggatc gagtgaccca gacaatcggt
     4141 tgccaccatg gataaatatc actttcttgg acctgataat tttcatgaaa accaatcatc
     4201 attgaaattc tgccgatcgg gtcgtaccaa acagaaggat taaaaataaa gaaactgccg
     4261 agggcgataa ttccgaagag cacgagtgag ccgaacaatt ctttccatat attccgttga
     4321 cgaactccgc tctcgattgc ggctgccagg atggcgaaag caattacaca ataaataatc
     4381 ttggatgctg cagtcattcc gagaaaaagt gctgaaagta ttaaccattt caaatt
//
LOCUS       DAOMIV010000114         4411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20404_length_4411_cov_3.677916, whole genome shotgun sequence.
ACCESSION   DAOMIV010000114 DAOMIV010000000
VERSION     DAOMIV010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20404_length_4411_cov_3.677916"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..1125
                     /locus_tag="PKV59_03825"
     CDS             67..1125
                     /locus_tag="PKV59_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP45913.1"
                     /translation="MKQKIFFVVCFLALFCLTFGSVFAEPVKITDILGREVTIEVPPA
                     LAVITGKKTSTITEVPFMFEYGKGHVAVPSESQDGGKFISFLENPLPELKDATVESVA
                     AMKPDVIFLKSYTREDAGIAYEETGIPVVYLSLESIDDYHTDVMNVGKIFGEEKRAEE
                     ILAYYDDIVKRVTEKASAIKEKKNVLLLQYSESNGAYSLKVAPAGWLQTTMVEMAGGE
                     PVWKEIGLNANSWTDVSFEQIAAWDPDVILVVNYFSDPKVSVEQLKNSTGWDQIRAVK
                     ENQIFPFAKDTFSWDSSSPRWGLGLLWTFKTLYPESAEDIDIDAEMLKFYRWFGLSDE
                     SIQANLIDLFHAEPQLTK"
     gene            complement(1216..2058)
                     /locus_tag="PKV59_03830"
     CDS             complement(1216..2058)
                     /locus_tag="PKV59_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQP45914.1"
                     /translation="MEKIPDWLKIWSELAEIRNTHFALQEEGQSDHWSENARKFSDRV
                     RERWQEPDTSRDFLKSVLFKNPESTLLDIGAGTGNWAIYLSPYAKKITALEPSAGMRA
                     VLEENLMLSEPKVDNVTVIAGKWPETDVPEHDFSLCSHAMYGIPNFEEAVRKMNAITR
                     KNCFLLIRAPLLNDPLAQIAQKVWGQPNDSPNFTVAYNALIQMGIYPDVLFEENRHIH
                     PKVYPSYESAFSDIKSRFGLENDSQFDTFIRSVLKDHLVQSENQWILQHNVKVGLISW
                     KSIR"
     gene            complement(2065..3195)
                     /locus_tag="PKV59_03835"
     CDS             complement(2065..3195)
                     /locus_tag="PKV59_03835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP45915.1"
                     /translation="MNSTDRNPKILIICMTRRGGLLQFSDCLVKNLAQLCPVAVVTAK
                     NAEHSEADWDQNTIAKFVLDMGEGKKGTFKKLFSLDTWKTLSKALNEFKPDIVHITSA
                     QEWNPFIGFYIRFIRRKPLVYTIHDVTYHEGIPAYFQITESLFRKIPNDLIVLTQKGK
                     EQLIQKGKKADRILVVPHGVYDFFTLYSKAEIEPQPQILFFGRIEPYKGLEILLKAAE
                     KVLKEHPDWKLHVAGGGDLTPYQQYLSNPQFIVTNRFLSNEEVALAMQQASIVALPYL
                     SASQSGVIPTAFAFRKAVIATNVGGIPDMVKNMETGILIPPNNVPALEKALNLLIESP
                     ELRRRLGENGYRFAHEELGWGAIAKKHVAFYRNILDRKKKGK"
     gene            complement(3205..>4411)
                     /locus_tag="PKV59_03840"
     CDS             complement(3205..>4411)
                     /locus_tag="PKV59_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45916.1"
                     /translation="FDFPEKQVLGEKTFIGYCLVCLLGFLLTALFLFLITRHQSTPNQ
                     KVAIQLIITGLIALILAGQPFWLTKSYLSFVFPNSRYTLPFLLGMSFFWTGILLLFPL
                     HRPAVRISICLLSAVMIGLSSGSHFMVANEYRRDWTLTKDFFHQLKWRIPAIEENTVV
                     ITNQLPIRFSTDNSLTAPLNWIYAQDYEKDSMPYMLYTNVKREVTLSKFEKGKKIYQE
                     YLSAKFEGNTEDAVSIYYNAPGCVHVLDPEVDVLNQTIPVIDRDAALLNNYSRILTDV
                     PYEPLDPVLFGEEPSKNWCWYYEHADLARQQKNWELVTKLGDEALLLDDHPNDPMERL
                     PFIEGYANSSQWDQAYRQTEEAMKVTPVMNNPLCALWNRIQNETIHSDEKEVTIQNVN
                     RLLDCSFLN"
BASE COUNT         1223 a          914 c         1000 g         1274 t
ORIGIN      
        1 taaaacacac tctgatctaa actttaataa ataaggattt attaattaag tattacggag
       61 ggaaatgtga aacaaaagat attttttgtg gtttgttttt tggcgttatt ttgcctgacg
      121 ttcggatcgg tctttgccga accggtcaag attacggaca ttttaggtcg tgaagtaacc
      181 atagaagttc ctcctgcgct ggcagtgata acgggtaaaa agacttctac catcaccgag
      241 gtcccgttca tgttcgaata cggaaaggga catgtggctg ttccgtccga aagtcaggat
      301 ggcgggaaat ttatatcttt tttggaaaat ccgcttccgg aactgaagga tgctacggtg
      361 gaatcggtgg cggccatgaa acccgatgtc atttttctga aaagttacac gcgggaagac
      421 gccgggatcg catatgagga aaccggtatt ccggtcgtct acctcagctt ggaatcgatc
      481 gatgattatc atacggatgt tatgaatgta gggaagatat tcggagagga aaaacgggct
      541 gaggagatct tggcttacta tgatgatatt gtcaaacgcg tcacggaaaa ggcctccgca
      601 atcaaagaaa agaaaaacgt cctattgctt caatactccg aatcaaacgg tgcgtattcg
      661 ttgaaggtcg ctccggcagg ttggttgcaa accacgatgg tcgaaatggc cgggggagaa
      721 ccggtatgga aagaaatcgg gttaaatgcc aattcttgga cggatgtcag ctttgagcag
      781 attgctgcct gggatccgga tgtcatttta gtggtgaact atttttccga tccgaaggta
      841 agtgttgaac aattgaaaaa ctccacaggg tgggatcaaa ttcgtgcggt aaaagaaaat
      901 cagattttcc cgtttgcaaa agacaccttt agttgggatt catcttctcc tcgttgggga
      961 ttgggactgc tctggacatt taaaacgctt tacccggaat ctgcggaaga tattgatatc
     1021 gatgcggaaa tgcttaagtt ttacagatgg tttggactga gtgatgaaag cattcaggcg
     1081 aatttgattg atctatttca tgccgagccg caactgacga aatagagcga agaaaagatt
     1141 aatactcaaa aaggatgtcg aaaaggcatc cttttttatt gaaatgatcg aatgaatacc
     1201 gattccctcc gtaagttaac ggatactttt ccaagatatc aaaccaactt ttacattatg
     1261 ctgaaggatc cattgatttt cactctgaac aagatgatct ttaagaacgg aacggataaa
     1321 ggtatcaaat tggctgtcat tttcaaggcc gaatcggctt ttaatatcac taaaagccga
     1381 ttcgtaggaa ggatagactt tgggatgaat gtgtcgattt tcttcgaata agacatcggg
     1441 ataaattccc atctgaatta aggcattata tgcgacagtg aaattggggc tgtcattggg
     1501 ctgtccccaa actttttggg cgatttgggc taatggatca ttgagcaacg gcgcgcgaat
     1561 cagtaaaaaa cagttctttc gggttatggc gttcatcttc cgaacagctt cctcgaaatt
     1621 cgggattccg tacatagcat gggaacatag tgaaaagtca tgttccggca catcggtttc
     1681 cggccattta cccgctatga ctgtgacgtt atcaactttt ggttcggaca gcatcagatt
     1741 ttcttcaagg acagctctca tccccgcaga gggctctaag gcggtgattt tttttgcata
     1801 gggagacaga taaatcgccc agttacctgt gccggcgccg atatccagca aagttgattc
     1861 cgggttcttg aataaaaccg attttaggaa atcacgtgag gtatcgggct cttgccaccg
     1921 ttctctcaca cgatcagaga attttctggc attttcgctc caatgatccg attgtccttc
     1981 ctcttgtaag gcaaaatgag tgtttcggat ttccgccaat tcgctccata ttttcagcca
     2041 gtccggtatt ttctccataa tcctttattt ccccttcttt ttccgatcta aaatatttcg
     2101 gtagaaagcg acgtgctttt ttgcgattgc tccccatccc agttcttcat gagcaaaacg
     2161 ataaccgttt tctcctaaac gccttcgcaa ttcaggggac tctatcaaca gattcaaagc
     2221 tttctcaagc gcgggaacat tattgggcgg tatcaatatc cccgtttcca tattttttac
     2281 catatcggga ataccaccga catttgttgc gataacagcc tttctaaagg caaaagcggt
     2341 aggaatgact cccgattggc ttgcggaaag ataaggtaaa gcaacaatgg aagcctgttg
     2401 catcgcgagg gctacttctt cattactgag aaaacgattt gtgacaataa attgcggatt
     2461 tgaaagatat tgttgataag gtgttagatc tccgcccccg gcaacatgaa gtttccaatc
     2521 cggatgctct ttcaaaacct tttcggctgc tttcagtaag atttccaatc ctttatacgg
     2581 ttcaatccgg ccgaaaaata gaatctgcgg ttggggttcg atttcagctt tgctgtaaag
     2641 cgtgaaaaaa tcatagacac cgtgcggaac aaccaagatc cggtccgctt ttttcccttt
     2701 ttgaatcagc tgttcttttc ctttttgggt taatacaatg agatcgttcg ggattttgcg
     2761 aaacaatgat tcggtaattt ggaaataggc gggaatccct tcgtgatagg ttacgtcatg
     2821 aatggtgtaa acaagcggtt tcctccgaat aaatcgaata taaaatccga taaagggatt
     2881 ccattcctgc gcagacgtaa tatgtacaat atccggtttg aattcgttca acgctttgga
     2941 taaggttttc catgtgtcga gactgaataa tttcttaaaa gtaccttttt tcccttcacc
     3001 catatccaaa acgaatttgg caatcgtatt ttgatcccaa tccgcttcgc tgtgttccgc
     3061 attctttgcc gtaacaacgg caacgggaca aagttgtgcc agatttttga ctaaacagtc
     3121 gctaaattgg agcaatccgc cccggcgggt catacaaatt atcaggattt tcggatttcg
     3181 atcggtagag ttcatctatc ctcgtcagtt gagaaagctg caatctaaca gccggttgac
     3241 attttgaatt gtaacttcct tctcgtcgga atgaatcgtc tcattttgaa ttcgattcca
     3301 caaagcacag agcggattgt tcattacggg tgtcactttc atcgcttctt ccgtctgccg
     3361 gtaggcttga tcccattgtg agctgttagc gtaaccttcg ataaaaggaa gacgttccat
     3421 cggatcgttc ggatgatcat ccaacaacaa agcttcatcc cctaattttg tgaccagctc
     3481 ccaatttttt tgttgacggg caagatcggc gtgttcataa taccagcacc aatttttaga
     3541 cggttcttcg ccaaataata ccggatcaag cggctcataa ggcacatccg tcagaattcg
     3601 gctgtaatta ttcaaaagag ccgcatcccg atcgatcacg ggaattgttt ggttaaggac
     3661 atccacttcc gggtcgagaa catggacgca gccgggagca ttgtaataga tgctgacggc
     3721 atcttcggtg tttccttcga atttggcgga aaggtattct tgataaatct tttttccttt
     3781 ttcaaatttg ctgagtgtta cctctcgttt tacatttgtg tagagcatat acggcatcga
     3841 gtctttttca taatcctgcg catagatcca atttaaggga gctgtcaaag aattatctgt
     3901 actaaagcgg atgggaagct ggtttgtaat gactaccgta ttttcttcaa tggcgggaat
     3961 tcgccatttc aattgatgaa agaaatcttt tgtcagtgtc caatcccgcc gatactcgtt
     4021 ggcaaccatg aaatgggatc cgctgcttag accgatcatg accgctgaga gcaagcatat
     4081 tgaaattctg accgccggcc ggtgcaatgg gaataaaagc agaataccgg tccagaagaa
     4141 agacattccg agcaaaaaag gcaaagtgta acggctgttc ggaaaaacaa aggaaagata
     4201 agattttgtc aaccagaaag gctgtcctgc caagatcaac gcaatcagtc ccgttattat
     4261 taattgaatc gcaactttct gattcggggt tgactggtgc cttgtgatca aaaaaaggaa
     4321 aagagcggtt aataagaatc cgagtaagca aaccagacag tagcctataa aagttttttc
     4381 cccaagtact tgtttttcgg gaaaatcgaa a
//
LOCUS       DAOMIV010000115         4404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20449_length_4404_cov_4.371350, whole genome shotgun sequence.
ACCESSION   DAOMIV010000115 DAOMIV010000000
VERSION     DAOMIV010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20449_length_4404_cov_4.371350"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKV59_03845"
     CDS             <1..309
                     /locus_tag="PKV59_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45917.1"
                     /translation="PDGIQSFSLDFGKGTDPEEWYVITNGINRQIFNPEKIGEWYLYG
                     MENGLYTLRLYVTSPIGTIAERRIVLSIDIPTKQDAETILVPVYGTEESIPDFFENYP
                     "
     gene            complement(366..1223)
                     /locus_tag="PKV59_03850"
     CDS             complement(366..1223)
                     /locus_tag="PKV59_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45918.1"
                     /translation="MSGKSYYFFGKVSKFHLLIMIIIVGFAVLLSGCQPKEEEIKSTV
                     ESAINATLKNIPTSTKLPSYTPNPTFTKLPTSTAYPTLTKLPTYTPPATYTVYPTFTK
                     LPTYTPLPTNTIYPTFTKLPTYTPLSTYTKLPTYTPLATYTNLPTYTPLPTGTPRNTY
                     TPLSTYTAYPTYTIYPTSTKLPTYTAQPTYTPVIRIVQVTPTVDSSILKADKTDGFYL
                     IGPEIAPGIWRTEPNKTKCYWKITGVKGEIISNYLGSGGGTIYIPKDAYQIEIQNCGK
                     VTFVQSINN"
     gene            complement(1394..1897)
                     /gene="nrdG"
                     /locus_tag="PKV59_03855"
     CDS             complement(1394..1897)
                     /gene="nrdG"
                     /locus_tag="PKV59_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HQP45919.1"
                     /translation="MCKVRLSGIVPESVCDGPGLRTVLFFQGCCHHCIGCHNPQTWPF
                     DGGEEFTVSDLLRTLPDSPLITGITFSGGDPFYQAEAAALIAKELHNRGKNIWAYTGF
                     KWEELLMESNSSRMKFLLECDVLVDGPFNNKEVKIGLIFRGSLNQRIILVKESFFKNR
                     IILWEPH"
     gene            complement(1908..4010)
                     /gene="nrdD"
                     /locus_tag="PKV59_03860"
     CDS             complement(1908..4010)
                     /gene="nrdD"
                     /locus_tag="PKV59_03860"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HQP45920.1"
                     /translation="MKNQYDKKDLLITENSLIALSEINCHSVKGEFEVIQKRDGRIVP
                     FDIEKIAVAIFKAAQAVGGSDQVLAHELAQEVIIYLQKETDKSKIPTVEGVQDAVEKI
                     LIETGHARTAKAFILYRSQRSRIREGKSELMDTVADILVETSRENANVGNSPSAKMLQ
                     IASAASRQYYLSRLIPEDISQAHRNGDIHIHDLDYYSKTLTCIQIPLRKLLHEGFNTG
                     HGYIRPPKRIGSAAALAAIILQSSQNDMHGGQSFAHFDRDMADFVFNADENEIYQAME
                     GLVYNLNSMHSRAGSQVPFSSINLGCDTSEAGRAITRNLLSAYEKGLGHEETPIFPNI
                     IFRIKKGINFDPQDPNYDLFKLAIRVSSSRMNPTFSFMDAPFNKPFDMEVAYMGCRTR
                     VISDRHGEPVSDGRGNLSFTTVNLPRLAIRAAGNIQKFYLLLEEVMTLTSRQLLHRFQ
                     IQSHLKVKDMPFLMGQGLYLGSEKLGSQDEIKEVIRHGTLSIGFIGLAETLIALTGYH
                     QAQSNDAQKLGLEIIEFMRKMTDYFSEKFDLNFTLLATPAEGLSGRFISIDKEKFGLL
                     PGVTDKDYYTNSFHVPVYYPISSFDKILIEAPYHAFTNAGHISYVELDAPPLYNPEAI
                     EAIIRHMNESGIGYGGINFPIDECPVCGFHGVIEGNCPVCSNTDIRRIRRITGYLSSI
                     DRFNDAKKAELKDRRPHK"
     regulatory      complement(4270..4393)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01689"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin-variant (AdoCbl-variant)
                     riboswitch is found in the 5'-untranslated regions of
                     vitamin B12 (Cobalamin) related genes in bacteria; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01689"
BASE COUNT         1284 a          841 c          901 g         1378 t
ORIGIN      
        1 cccgatggaa tacaatcttt cagccttgat tttggaaaag ggacggatcc ggaagaatgg
       61 tacgtgatca cgaacggaat taaccgtcag atttttaatc cggaaaagat cggcgaatgg
      121 tatttatatg ggatggaaaa cggactttat actttaaggc tatatgttac aagcccaatc
      181 gggacgattg ccgaacgaag aattgtattg tcgatcgata ttcctacaaa acaggatgcc
      241 gaaacgatat tggtacccgt ttatgggacg gaagaatcaa tacctgattt ttttgaaaat
      301 tatccataaa ataaacaggc caaattcttt tagggcctgt ttatttatct tatttgtcag
      361 gaatgttaat tatttattga ttgcacaaaa gtgacctttc cacaattttg gatttcaatt
      421 tgatatgcat ctttgggaat atatatcgtt ccgcctccgc tgcctaaata attggaaata
      481 atctcccctt taactccggt tattttccaa taacattttg ttttattcgg ttcggtcctc
      541 caaattcccg gagcaatttc cggaccgatc aagtaaaagc catccgtctt atccgctttt
      601 agaatactac tgtcgactgt cggtgtaact tgaacaatcc gaataaccgg agtgtaagtc
      661 ggttgagcgg tataggtagg taatttagta gaggtcggat atatcgtata agttggatag
      721 gctgtgtaag tggaaagggg tgtgtaagtg tttcgggggg taccggtcgg caatggagta
      781 tatgtcggca aatttgtata cgtagccaag ggagtataag ttggaagttt ggtatatgtt
      841 gacagcggcg tataggtagg taatttcgtg aaagtcggat agatcgtatt ggtcggtagc
      901 ggcgtatagg tcggtaattt tgtaaaagtc gggtagacgg tataagtagc cggcggagta
      961 taggttggta attttgtaag cgtgggataa gccgtacttg tcggcaattt tgtaaaagtc
     1021 ggatttggag tataggatgg taattttgta gaagtcggaa tattttttaa ggtcgcatta
     1081 attgctgatt ctacagtcga ttttatttct tcttccttgg gttgacaacc ggaaagtaaa
     1141 acggcaaaac caacgatgat gatcattatc agcagatgga attttgagac ttttccaaag
     1201 aaataataac tttttccaga cattttgtct cctttctaaa ggccaatcaa ctgtaaatct
     1261 ttttggactt ttaaatttta ccaaaatatt tcatcatgaa agaaaactct tgttatcctc
     1321 gatcttaatc aggataaaag gcatgaaaac atatgacaaa aaacactgaa atagtggtaa
     1381 ttatattctc tgattaatgt ggttcccata aaatgatcct atttttgaaa aaactttcct
     1441 ttaccaggat aattctttgg ttcaaagatc cgcgaaaaat cagccctatt ttgacttctt
     1501 tattattaaa tgggccgtct accagtacat cacattcgag aagaaatttc atgcgggacg
     1561 aatttgattc cattaataat tcttcccatt taaaacctgt atatgcccaa atgttttttc
     1621 cgcgattatg taactctttt gcgatcagcg cggcagcctc ggcttgataa aacggatcac
     1681 ctccactgaa agtaattcct gttattaatg gggaatcagg cagcgttctg agcaaatccg
     1741 aaaccgtgaa ttcttctcct ccgtcgaaag gccaggtttg aggattgtgg cagccgatgc
     1801 aatggtggca gcatccttga aaaaacagta cagtacgaag tccgggtcca tcgcaaacac
     1861 tttccgggac aattcccgat aatcgaactt tacacataat attattccta tttatgcgga
     1921 cgacgatctt ttaattctgc tttctttgca tcattaaatc gatcgatgga agagagataa
     1981 cctgtgatac ggcggatccg ccggatatcg gtattgctgc ataccgggca gtttccttcg
     2041 attacgccat gaaagccaca aacgggacat tcatcaatcg ggaaattaat tcctccgtag
     2101 ccgattccgc tttcattcat atgtcgaatt attgcttcga tcgcttccgg attataaagc
     2161 ggcggtgcgt ctaattccac ataactgata tggccggcat ttgtgaaagc atgatagggt
     2221 gcttcaatta aaattttatc gaatgatgaa atcggataat aaaccggaac atgaaagcta
     2281 tttgtataat aatctttgtc ggttacgccc ggcaataaac cgaatttttc tttatcgatc
     2341 gagataaaac gtcctgaaag tccttcggca ggcgttgcca atagtgtgaa attcagatca
     2401 aacttttccg aaaaataatc cgtcattttc ctcataaact ctattatttc cagtcctaat
     2461 ttttgagcat cgtttgattg agcttggtga tatccggtta atgcaatgag tgtttccgct
     2521 aatccgataa atccaatgga aagtgtcccg tggcggatca cttctttgat ctcatcttgg
     2581 ctgccgagtt tttccgatcc caaatataga ccctgtccca tgagaaaagg catatctttt
     2641 actttaaggt gtgattgaat ttgaaatcga tgcagaagtt gacgagaggt aagtgtcata
     2701 acttcctcca gaagaagata aaatttctga atatttcctg cggcacgaat ggcaagtcgc
     2761 gggagattga cagtggtaaa agataagttt cctcgtccgt cacttaccgg ttctccatgt
     2821 cgatccgaaa taacgcgtgt gcggcagccc atataggcta cttccatatc aaaaggcttg
     2881 ttaaacggtg cgtccatgaa ggaaaacgtc ggattcatcc gggaagaaga aactcttatt
     2941 gccaatttaa aaagatcata atttggatct tgcggatcga aattgatgcc ttttttaatt
     3001 cgaaatataa tattagggaa gatcggtgtt tcttcgtgcc ctaaaccttt ttcataagct
     3061 gacaagagat tgcgggtaat cgctcgtccg gcttcgcttg tatcacaacc cagattaatt
     3121 gaactaaacg gaacttgaga accggcgcgg ctatgcattg aatttaagtt atatactaat
     3181 ccttccattg cctgataaat ttcattttcg tccgcattga aaacaaaatc cgccatgtca
     3241 cgatcaaagt gagcaaagga ttgaccgccg tgcatatcat tttgcgaact ttgcaaaata
     3301 atagccgcaa gagcagcggc cgatccgatt cgtttcggag gacgaatata tccatgtccg
     3361 gtattaaagc cttcatgcaa tagttttctt aaaggaattt gaatacatgt caacgttttt
     3421 gaataataat ccaagtcatg aatatggata tcgccgttgc gatgcgcttg agaaatatct
     3481 tcgggaatca atctggaaag atagtattgt ctgcttgctg cggaagcaat ttgaagcatt
     3541 tttgctgaag gtgagttacc gacattcgcg ttttctcggc ttgtttcaac aagaatatcc
     3601 gccacggtat ccattaattc acttttccct tcgcgaatac ggctccgttg ggaacgataa
     3661 aggataaatg cttttgcggt tcgggcatga ccggtttcaa ttaatatttt ttcaacagca
     3721 tcctgaacac cttcaacggt cggtattttc gatttgtccg tttctttctg taaatatata
     3781 atgacttctt gagccaattc atgtgctaaa acctgatcgc ttcctccgac agcttgagcg
     3841 gctttaaata ttgccacagc tattttttca atatcaaagg gaacgattcg tccgtccctt
     3901 ttttgaataa cttcaaattc tccttttact gaatgacagt taatttcaga caaggcaatt
     3961 aaagaatttt cagttataag cagatctttt ttatcatatt ggtttttcat tttatgcctc
     4021 atttacgtgt ttcgtcgaat ttctttttaa tcgatcaaaa taaattctta agtttcaaat
     4081 attatgcaaa atggagcaaa taaaaactgg cgttttactt gccagcattc aaaattcagc
     4141 gagaactttt gtaaaaaaag ttttcgtatt atataaataa tcattgaaat tatgagaaca
     4201 taagcaactc ctcgtaaatt gattaaaaaa gctcacccta atgaaggatg agctttatga
     4261 gaaagttgct ttacaactgt ttttgctcaa ccctttaacg cgaagggcgt tttaaccaca
     4321 gacaggcatt cgggctttaa ccgttgcgcg acagcgccgg aatttcaccg gactttccct
     4381 gatatttcaa tcatccacga caga
//
LOCUS       DAOMIV010000116         4385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20574_length_4385_cov_4.648037, whole genome shotgun sequence.
ACCESSION   DAOMIV010000116 DAOMIV010000000
VERSION     DAOMIV010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20574_length_4385_cov_4.648037"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PKV59_03865"
     CDS             <1..1135
                     /locus_tag="PKV59_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP45921.1"
                     /translation="NSVTVRDNGRGIPTDIHPQMKISALEVVLTTLHAGGKFGGGSYK
                     VSGGLHGVGSSAVNALSEWMEAKVWRDGKIYFQRYNRGKPEERVKVIGECPEDQTGTE
                     ISFLFDKEIFKEEPGYRYETLVNRFREMAFVTKRTKIHLVDEKDNKETTFYFQGGISS
                     FVHYLNKDNVPLHPIVHAEKTIENVGVEVALQYTDVYTETVYSFANTINTIDGGTHLT
                     GLRTAITRVINDYARSANLLKESDPNFSGDDTREGLTAVVSIKHPDPQFESQTKVKLM
                     NTEVSTYVQQVIGECFKDFLENNPAAAKAIVNKCLTSSRARDAAKKARDLVIRKSALE
                     SMTLPGKLADCSGGITFHNIKFRLFGISSGTVSQFSLESMTLP"
     gene            1139..1828
                     /locus_tag="PKV59_03870"
     CDS             1139..1828
                     /locus_tag="PKV59_03870"
                     /inference="COORDINATES: protein motif:HMM:NF013878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HQP45922.1"
                     /translation="MADCSGRNPEQTELYIVEGDSAGGSAKQGRDRGFQAILPLRGKI
                     LNTERARLDKILSNNEVKAMISAIGCGIGENFDIKGLRYGRIIIMTDADVDGSHIRTL
                     LLTFFFRYMPELVEQGHLFIAQPPLYRTTYKGVHRYAYSDAEQAAILKELGSGSEKAS
                     IQRYKGLGEMNAEQLWETTMNPAYRTLLKVTVDDASQAAKTFEMLMGDEVPPRTRFIQ
                     THAHEVRNLDI"
     gene            complement(1982..3040)
                     /locus_tag="PKV59_03875"
     CDS             complement(1982..3040)
                     /locus_tag="PKV59_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP45923.1"
                     /translation="MKSANNIGFGIIGLGMIAEFHAKAIENIEGCKFVAGYDHTPAHS
                     ESFCALHGGTPYTDLEKFLADPDLDIVTIATPSGFHLEGAVRAANAGKHIIVEKPLEI
                     STERCDKIIEAAVKNNVRMCTVFPSRFHDSARLIKEAVRQGRLGKIVLADAQIKWYRS
                     QEYYDSGGWRGTWKFDGGGVLMNQGIHAIDLLQWVMGDVSEVTAYTATLAHERIEVED
                     TAVAILKFSDGALGMIEGTTAAYPGFLKKLEICGSKGSVMMEEESITKWEFSEPMPED
                     QKIRERFQNNTQTGGGAGDPKAISFEGHRKLFSAFVDAVIRNKPFDLEGNEGRKSVEI
                     IENIYKSAKNGVSVKFRS"
     gene            3407..>4385
                     /locus_tag="PKV59_03880"
     CDS             3407..>4385
                     /locus_tag="PKV59_03880"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQP45924.1"
                     /translation="MAVKHNAEYENSELYKIRHSTAHIMAEAVLEKFPEGKVAIGPAI
                     ADGFYYDFDLPRALTPEDLEEIEHRMKKIIKGNHPFIKEVISAEEAKKIFADQPYKIE
                     LIEGLEKGGMDEDGNPTDEKPEISIYKQDKFVDLCRGPHVSNTSEINPNAIKLLNVSG
                     AYWRGDEKRPMLQRIYGTAWETEAELKDYLWKQEEAKKRDHRKLGKELDLFSVSDEVG
                     QGLILWHPKGAKVRKLIENYWSEEHEKWGYDFVYTPHIGKSQLWETSGHLGFYSENMY
                     SPIDIEGTKYYIKPMNCPFHLYIYKSNLRSYRDLPIRYAEMGTVYRYERS"
BASE COUNT         1243 a          925 c         1056 g         1161 t
ORIGIN      
        1 taacagtgta actgttcggg ataacgggcg cggaatcccg acggatattc atccgcaaat
       61 gaaaatttcc gcattggaag ttgtgctgac tacgttgcat gcgggcggta aattcggcgg
      121 cggatcatat aaagtctccg gcgggctaca tggtgtcggt tcttcggcgg tcaatgcatt
      181 gtccgaatgg atggaagcca aagtttggcg ggacggtaag atttattttc aacgttacaa
      241 tcgcggaaaa ccggaagaac gggtgaaggt catcggcgaa tgcccggaag atcagaccgg
      301 tacggaaatc agtttcctgt ttgataagga aatatttaaa gaagaacccg gataccggta
      361 tgaaaccttg gtaaaccgct ttcgggagat ggcttttgtc acaaagcgga ctaaaatcca
      421 tttggtggat gaaaaagaca ataaagagac cactttttat tttcagggtg gaatcagttc
      481 atttgttcat tatctgaata aggataacgt cccgcttcat ccgattgttc atgcggaaaa
      541 aacaattgaa aacgtcggag tggaagttgc cctgcaatat acggatgttt atacggaaac
      601 ggtttattcg ttcgccaaca ctatcaatac aattgacggc ggaacccatc tgaccggatt
      661 acgaacggca attacgcgtg taatcaatga ttatgcccga agcgccaatc tattaaagga
      721 atccgatcca aatttttcag gtgatgatac gcgggaaggg ctgacggcgg tggtcagcat
      781 caagcatccc gatccgcaat ttgaaagtca gaccaaagtg aaattgatga acaccgaagt
      841 ttcgacatat gtgcagcagg tgatcggtga atgcttcaaa gattttttgg agaataatcc
      901 cgcagctgcc aaagcgatcg tcaataaatg tcttacttca tctcgggctc gcgatgctgc
      961 caaaaaagcc cgtgatttgg taatccgaaa atcagcctta gaaagtatga cgcttcccgg
     1021 aaaattggct gactgttccg gcggaatcac cttccacaat ataaagttcc gtctgttcgg
     1081 gatttcttcc ggaacagtca gccaattttc cttagaaagt atgacgcttc cctgaaaatt
     1141 ggctgactgt tccggaagaa atcccgaaca gacggaactt tatattgtgg aaggtgattc
     1201 cgccggcgga tcggcaaagc agggacgtga tcgcggtttt caggcgattt tgcccctccg
     1261 tggaaaaata ttaaataccg aacgcgccag gctggataag attttatcga ataatgaagt
     1321 gaaagcaatg atttcggcca tcggttgtgg gatcggtgag aattttgaca tcaaagggtt
     1381 gcgatacggc cggatcatta ttatgaccga tgccgatgta gacggaagcc atattcgaac
     1441 attactgctt accttctttt tccgctatat gccggaattg gtcgagcaag gacatttatt
     1501 tattgcccaa ccgcctttgt atcgaacgac ctataaaggt gtacatcgat atgcttactc
     1561 cgatgcagag caagcggcaa ttctgaagga actcggcagc ggatcagaaa aagcttccat
     1621 tcaacgctat aaagggttgg gagaaatgaa tgcggagcag ttatgggaaa caaccatgaa
     1681 cccggcatat agaacgcttc taaaggtcac cgttgatgat gcttcccagg cagccaagac
     1741 cttcgaaatg ctgatgggtg atgaagttcc gccaagaaca cggtttattc agacccatgc
     1801 gcatgaagtc aggaatttgg atatttgaaa ttcaggtaaa cgaggctaaa aacagatcgt
     1861 ttttgatcag aaaatgacac tcccggaatt cggaaatcaa aaccgaagtt cgggagtgtt
     1921 ttatgattgt tttgtgtctt taactttttt cttcccccga tttcatgact gggtttcaac
     1981 tttatgatcg aaattttacc gaaacgccgt tttttgcact tttgtagata ttttcaatga
     2041 tttctaccga ttttcgacct tcatttcctt ccagatcgaa aggcttattc cggataacag
     2101 cgtccacaaa ggcgctgaaa agcttgcgat gcccttcgaa actgatcgct ttcggatcac
     2161 cggctcctcc gccggtttgg gtattgtttt gaaatcgttc gcggattttt tgatcttccg
     2221 gcatcggttc ggaaaactcc catttggtga tactttcttc ttccatcatc acactgcctt
     2281 tggatccgca tatttctaat tttttaagga atccgggata cgccgctgta gtcccttcaa
     2341 tcattcccaa agccccatcg gaaaatttca ggatcgcgac agcggtatct tctacctcga
     2401 ttctttcatg agccaatgtg gcggtataag ctgtaacttc ggatacgtcg cccatcaccc
     2461 attgaagcag atcaatggca tgaattcctt gattcattaa aacaccgccg ccgtcaaatt
     2521 tccaagttcc tctccaaccc cccgaatcat aatattcttg cgaccggtac catttaattt
     2581 gggcatcggc taatacgatt tttcccagcc tgccttgcct gacggcttcc ttgattaaac
     2641 gggcggaatc atgaaagcgg gaggggaaga cggtacacat cctgacattg tttttaacag
     2701 ctgcttcaat aatcttgtca caacgttcgg tggatatttc caagggtttt tcaacgataa
     2761 tatgtttgcc tgcattcgct gcgcgaacag ctccttcaag gtgaaaaccg gacggagtcg
     2821 ctattgttac aatatcaagg tcgggatcag ccagaaattt ttccaaatcc gtataagggg
     2881 tcccgccatg gagagcgcag aaggattcgg aatgagccgg cgtatgatca tacccggcga
     2941 cgaatttgca gccttcgata ttttcaatgg cttttgcgtg aaattctgcg atcatgccca
     3001 aaccgataat accgaaaccg atattgtttg ctgatttcat gaggactcct tatagtgaaa
     3061 aaatgcttta tcgaattatt tttatctgcg gtaactcatt gtgtcataat gttttacaaa
     3121 gagcgacaga tcatgtataa ttttatccgg aagcgttgaa cagaacaatg cgtcagacca
     3181 gtcgcacaga gagaccggaa agaaggtgag aatcggtccg aaggagtctg acgaaaacca
     3241 tctgggagct cgctgccgaa ctgattaagg cagccggtga aataccgata tcatttatct
     3301 gagcgggtcg ctttatgaga gtgatcaagc tgggtggaac cacgagactt tacagactcg
     3361 ccccggtaat ggggtgagtt ttttttattt ttattacgga ggatatatgg ctgtaaaaca
     3421 taacgctgaa tacgagaatt cggaattgta taaaatcagg cattccactg cccatattat
     3481 ggcggaggct gtccttgaaa aatttcccga agggaaagtg gcgatcggac cggcaattgc
     3541 ggacggtttc tactatgatt ttgatcttcc gagagcgctg acgcccgaag atttggaaga
     3601 gatcgaacat cggatgaaaa aaattatcaa gggcaatcac cccttcataa aggaagtcat
     3661 cagtgcggaa gaggcaaaga agatttttgc cgatcaacct tataaaatcg agttgattga
     3721 agggcttgaa aaaggcggga tggatgagga cgggaatccg accgatgaaa aacctgagat
     3781 atctatttat aaacaagata agtttgtcga tctttgccga ggtcctcatg tttccaatac
     3841 ctccgaaatt aatcccaatg cgattaaatt attgaatgtt tccggtgctt attggcgggg
     3901 agatgaaaaa cgcccgatgc tgcaaagaat ttatgggacg gcatgggaga ccgaagcgga
     3961 gctgaaagat tatctgtgga aacaggaaga agccaagaaa cgcgatcacc gaaaacttgg
     4021 gaaagaactg gatttgttca gcgtcagtga tgaggtgggg cagggtttaa tcttgtggca
     4081 tccaaaaggc gctaaggttc gaaagctgat cgaaaattat tggagcgagg aacatgaaaa
     4141 atggggatat gatttcgtat ataccccgca tatcggaaaa tcccagctct gggagacatc
     4201 cggtcacctc ggtttctata gcgagaatat gtattctccg attgatatcg agggaacgaa
     4261 atactatatc aagccgatga actgcccgtt ccatctgtat atttataaga gcaatttacg
     4321 ttcttatcgc gatttaccga tccgatacgc cgaaatggga acggtttacc gctatgaacg
     4381 aagcg
//
LOCUS       DAOMIV010000117         4383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20590_length_4383_cov_4.944085, whole genome shotgun sequence.
ACCESSION   DAOMIV010000117 DAOMIV010000000
VERSION     DAOMIV010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20590_length_4383_cov_4.944085"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1829
                     /locus_tag="PKV59_03885"
     CDS             261..1829
                     /locus_tag="PKV59_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQP45925.1"
                     /translation="METILSLYERTLSEKNPDTTAIRWVDSHLNMTPITWRMLENESN
                     NRAIYLTQLGCRPGHHVFIFLESSIEIWYFFWAALKCGAVPCVLFPDFGSDALGARLQ
                     SGRADWIITDRWNEKNIGAIRSCFSLKKIILLEEPSGIPDTRMALFHRESTTNDSFPL
                     YPAIETDPAFIVFTSGSTGFPKAVVHTHRCAESILRSMQNVLQVRNDDIYWCNAHPAW
                     ITGTVYGVIGPLITDVESVQYSGNFHARRWMPILQNQEVSVWYSAPTAFRSLMTQPIE
                     FFTKFDFSNLREIFSIGEPLNPAVYYWGKEIFQREIRDTWFQTEAGTIRIANLPGKLI
                     KPGYMGMPVDDALPVILDEQGKECSAGQVGRLCLKAGWQSCFTDYFGQHDAYLSKFQN
                     GYYQTGDLAFQDMDGYFCFVGRDDDVINTSGHLVGPFEVESVLLEQPEIAEAGVVAAP
                     DPLLYEKVAAFIVLKTGVEWTSELETRLAVAVTTRLSTQATPRQFIVVNALPKNPAGK
                     ILRNELRKRLRSQS"
     gene            complement(1893..3098)
                     /locus_tag="PKV59_03890"
     CDS             complement(1893..3098)
                     /locus_tag="PKV59_03890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQP45926.1"
                     /translation="MKKLSAGNLFWNQTIEPFACFNPLQNDLETDVLIIGGGMSGNLC
                     AHEIAGRNHSVVVCEKDQLAMGSSSASSGILQYLNDEMLYRLIESIGEKKAVLFYQMC
                     LDSMKDLINLSRKMEQYGGLMPRKGVFFASAKEDVPELDKEYKALSDHYFPVSYLDHA
                     ELMRIYGIDRPAALETSMNAEVNPYQFLQTLMKLNVSLGVQYYEKTGIKKVKTDKDQI
                     IATTKNGNRIQAKYVVFATGCSVIYPELKKKMKVVNTYTFASSVSKRPLWQDHVLFRE
                     TRPPYLFFRMTEDNRVIAGETDKKADSPEKKKGAIDKRNREILERVQSLMPNLDVKVE
                     YSWNTLSADSKDHIPFIGPSKKDPRRIYLIGVGGNGLVHSMAGASIIADYIDGIKNPY
                     AGVVKAKRK"
     gene            complement(3215..>4383)
                     /gene="pnp"
                     /locus_tag="PKV59_03895"
     CDS             complement(3215..>4383)
                     /gene="pnp"
                     /locus_tag="PKV59_03895"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQP45927.1"
                     /translation="AQEIDSLSPVENKRYIHHYNFPPFCTGEVKRLGGQSRREIGHGA
                     LAERALVPVIPSEEDFPYTIRLVSEVMSSNGSSSMASVCGSTLALMDAGVPIKAPVTG
                     IAMGLIKEGDDYVILSDIQGAEDHLGDMDFKVAGTEKGITALQMDIKIRGITTEIMRK
                     ALDQANEGRHFILGKILEVIPAPRKDLKPHVPRLITVQVPIDKIGAIIGPGGKNIRTL
                     QEATNTKVDIDENGLVYISATNVGDSDAARDQILSMIETAIVGNIYTGKVVRVTDFGA
                     FVEILPNTDGMVHISQLDSERVNKVEDIVNVGDEITVMVTGIDENGKIRLSRQAVLEG
                     WTLDQAVAADSKKPSSGHRPGNSDRSRHNSDHKSGNRGDDDRRHSGGGSYKKQR"
BASE COUNT         1142 a         1075 c          952 g         1214 t
ORIGIN      
        1 cgtcggggac ggcgatcccg ctccgaatcc tccctcaccc tgaccctctc cccaagggcg
       61 agggaaattg cacaagcgaa actgttggca aaaatttgcc tcgcatcaga aaatattccg
      121 atcgaaatga gacaaatgaa attatccgtt acaataactg cagatcgatt atcccgcaac
      181 ccgtgaattt atattcgtta tttttgtcca attatcggaa ggggattaaa tcgataaatt
      241 aattcaaaaa gaagcgaact atggaaacaa ttctctctct ttatgaacga acactttcag
      301 aaaaaaatcc ggatacgacg gcaattcgct gggtagacag tcatctgaat atgacaccga
      361 taacctggcg tatgctcgaa aacgaaagca acaatcgggc aatctatctg acacaactgg
      421 gttgccgtcc cggacaccat gtttttatct tccttgagag ttctatcgaa atttggtatt
      481 tcttctgggc cgctttaaaa tgcggagcgg ttccttgtgt cctattcccc gatttcggct
      541 ccgatgccct cggagcccgc ttacaatccg gtcgggcgga ttggatcatt accgatcgtt
      601 ggaatgaaaa gaatatcggt gcgattcggt catgtttctc gctgaaaaaa ataattctgc
      661 tcgaagaacc ctccggtata ccggacactc gaatggcttt gtttcatcgg gaaagcacaa
      721 ccaatgactc ttttccgctt tatccggcca ttgaaaccga tccggcgttt attgtcttta
      781 cctcggggtc gaccggattt ccgaaggcgg ttgttcatac ccatcgatgt gccgaatcga
      841 tattgcgttc catgcaaaac gtgctccagg ttcggaacga tgacatttat tggtgtaacg
      901 cgcatccggc ttggattacc ggcaccgtat acggtgtgat cggccctttg attaccgatg
      961 tggaatccgt tcaatacagc gggaatttcc atgcccgacg atggatgccc attcttcaaa
     1021 atcaggaagt atctgtttgg tattcggcgc cgaccgcttt tcgttctctg atgactcaac
     1081 cgattgaatt ttttacaaaa tttgatttca gcaatttacg ggaaatattc agtatcggcg
     1141 aaccgctgaa tcccgccgtt tattattggg ggaaagagat ctttcaacgt gaaatccgcg
     1201 atacatggtt tcagacggaa gccggcacga tccgtattgc gaatcttccc ggtaaattaa
     1261 ttaaacccgg ctacatgggc atgccggtgg atgacgcatt acccgtcatc ctggacgagc
     1321 aggggaaaga gtgttcggcg ggtcaagtcg ggagactttg tctcaaagcc ggttggcaat
     1381 cttgttttac cgattatttc ggtcagcatg atgcatatct ctccaaattt caaaacggct
     1441 attaccaaac cggagatctg gcttttcagg atatggacgg atatttttgt tttgtcgggc
     1501 gggatgacga tgttatcaac acttccggac atttagtcgg cccttttgaa gtggaaagtg
     1561 tgttgctcga acaaccggaa attgccgaag cgggtgtggt ggctgcgccg gatccgctgt
     1621 tgtatgagaa agtagctgca tttattgtat tgaagacagg ggtggaatgg acatccgagc
     1681 tcgagacccg tctggcagtt gccgtaacta cccgtctctc gacccaagct acgccgcgac
     1741 aattcatcgt cgtcaatgct ttaccgaaaa atccggccgg gaaaattctg cgtaacgaat
     1801 taaggaaaag actccggtcc caatcttaaa ataattcccc cgggcagccg gcaaaaacgg
     1861 aacggatgct cagaaaaaat caaaggcggg atctattttc gctttgcttt taccacaccg
     1921 gcgtacggat ttttgattcc gtctatataa tccgcaatga tacttgctcc ggccatcgag
     1981 tggacaagac catttccgcc gacaccgatc agatagatcc ggcgcggatc cttttttgaa
     2041 ggaccgatga agggaatatg atctttggaa tcggcggaca gcgtgttcca actgtattcg
     2101 actttgacat ctaaattcgg catcaaactc tgcacccgtt ccaaaatctc ccgatttctt
     2161 ttatctatgg cacctttctt tttttccggc gagtcagcct ttttatccgt ttctcccgcg
     2221 atcacccgat tgtcttccgt catgcggaaa aataaataag gaggtcgtgt ctcgcggaat
     2281 aatacgtgat cctgccagag cggtcgtttt gaaaccgaag aagcaaaagt ataagtattt
     2341 acaactttca ttttcttttt taattccggg tagataaccg aacatccggt cgcaaaaaca
     2401 acatattttg cttgaattcg gttcccgttc tttgttgttg ctatgatttg gtccttatcc
     2461 gttttcactt tctttattcc ggttttttcg taatattgaa ctccgagaga aacattcagt
     2521 ttcattaatg tctgcaaaaa ctgataggga ttcacttccg cattcatcga cgtttcgagc
     2581 gcggcaggtc tgtcaatacc gtaaatccgc atcaactcgg catgatccaa ataagaaacc
     2641 ggaaaataat gatcggataa ggctttgtat tctttatcga gttcggggac gtcttctttg
     2701 gcgctcgcaa aaaagacccc ttttcgcggc attaatcctc cgtattgttc catttttcgg
     2761 ctgaggttaa tcaaatcttt catactgtca agacacattt gataaaaaag cacggctttt
     2821 ttttctccga ttgattcaat cagtcgatat aacatctcat cattcaggta ctgaaggatt
     2881 ccggaacttg ccgaagagct acccatcgct aactgatctt tctcacaaac aacaacggaa
     2941 tgattgcgac cggctatttc atgtgcacag agatttccac tcattccgcc accgataatc
     3001 aaaacatctg tttcgagatc attttgaagc ggattaaaac aggcaaaagg ttcaattgtc
     3061 tgattccaga acagattccc cgcggataac tttttcatag gccctccatc cgacataaat
     3121 tctattattt ataaatcata cccttcaaat aaaaaaaaga gcaacctctt tttgaaaggt
     3181 tgctcaaaaa tatgatcgct tttgaaaaaa tgaatcagcg ttgtttctta taggatccgc
     3241 cgccgctatg tcgacgatca tcatcaccac gattcccgct cttatggtcg ctgttgtgcc
     3301 tgctgcgatc actgttaccg ggacggtgtc cggaagaagg ctttttggaa tcagccgcaa
     3361 ccgcttgatc caaagtccac ccctccaaaa cggcttggcg ggaaaggcga attttaccat
     3421 tttcatcgat accagtcacc atgacggtga tctcatcgcc cacattgaca atatcctcca
     3481 ctttattaac tcgttcggaa tccaactgcg aaatatggac catgccgtcg gtattgggaa
     3541 gaatctcaac aaaagcgcca aaatcggtaa cacgcactac tttaccggtg tagatattgc
     3601 cgacgatcgc ggtttcgatc atggaaagaa tctgatcacg agcggcatcc gaatctccca
     3661 catttgtggc ggaaatataa accaacccgt tttcatcgat atccactttt gtattcgttg
     3721 cttcctgcaa tgtgcgaata ttctttcccc cggggccgat aatcgcgccg atcttatcga
     3781 tcggaacctg taccgtgatc aatcgcggaa catgcggttt caagtctttg cggggagcgg
     3841 gaatcacttc cagaatctta cccaagataa aatgacgacc ttcatttgcc tgatccaacg
     3901 ctttacgcat tatttcggtc gtaattccgc ggattttgat atccatttgg agcgcggtaa
     3961 tccctttttc ggttccggcg actttgaaat ccatatctcc caaatggtct tcggcacctt
     4021 gaatatcgga aagaattaca taatcgtccc cttctttgat caaccccatt gctataccgg
     4081 tgacgggtgc tttaatcgga acgcccgcat ccatcaatgc caatgtagat ccacagaccg
     4141 atgccatcga actggaaccg ttggagctca tcacttcgga aaccaagcga atggtataag
     4201 gaaaatcttc ttcggaaggg ataacgggga caagcgcccg ttccgccaaa gcaccgtgtc
     4261 cgatttcgcg tcgactctgc ccgcccagcc tcttcacttc accggtgcag aacggcggaa
     4321 agttgtaatg atgaatatac cgtttatttt ccacgggcga aagcgaatca atttcctgcg
     4381 cat
//
LOCUS       DAOMIV010000118         4379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20614_length_4379_cov_4.781452, whole genome shotgun sequence.
ACCESSION   DAOMIV010000118 DAOMIV010000000
VERSION     DAOMIV010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20614_length_4379_cov_4.781452"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PKV59_03900"
     CDS             <1..1093
                     /locus_tag="PKV59_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP45928.1"
                     /translation="PYLGILIEYPFQNDETIFTKNTVQLREPSLPQPVNLGVIGAGNY
                     ANATFLPILKSAGKSIRLVTIVSNRGASAREAAEKFGFEKAGVNAETVLNDPDINTVV
                     ILTRHNLHSSMVIEALNRQKNVYCEKPLALDFDSLQAVYQARQANPKPILMVGYNRRF
                     APLAVKLKNFLANCAEPLAMHYTVNAGFIPLTHWTQDIEIGGGRILGECCHMLDFMLF
                     LTQSRPLQVNTIALPNNGRYQDDNVSIQVKFENGSIGTVAYLANGDKSYAKERLEVFG
                     GGKIAILNDFKTLETWDRGNHHRTNSPLRIDKGHAASWQAFVSAIINGEHEPIPTEQL
                     FYSSLTSLAAVDSLHSNEIVAVPDLNIFL"
     gene            1134..3128
                     /locus_tag="PKV59_03905"
     CDS             1134..3128
                     /locus_tag="PKV59_03905"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HQP45929.1"
                     /translation="MMNPISKKLKALRSFGIKRLWYYGTYTLKVKSGFFQKRSIPKNS
                     KNNKKTVAFSPLPNGRKDEILPFLLADSKSILESAGEIITVGYRPYSGNIIRSLDLVP
                     PHKDLFWTRIPPRENLDIKDIWEPARFCWVTALCRAYVLTGDRIYPEFFYRQVQTFIE
                     SNPPYYGENWVSAQEAAIRMISVTFAAGILQPSLPDKNSTFPDLPAFIALHATRIMQT
                     RSYAKAQNNNHRLSEAAGLMTAACVLPKSPDASKWFSTGWKDFQNALNSQILPDGAYI
                     QYSVNYHRLMLQLVLWVDSLLKTQKIAWPANLIRKIRRSVLWLAKHTDEFSGQACNTG
                     HNDGSLLFALGGNYNDFRPTLQTAAVVFLGKPLFPPGPWDEMRIWLGCYNRAGVDPDS
                     QFESTFYSQNVQRISAGNQWGILHTAGFKDRPAHADQLHVSLWKGGKCMALDAGTYRY
                     TAAPPWNNRLKSAFVHNCLTIDCQEPMIDAGKFLWLDWDQARILDQTEFRKIAEHSGY
                     KRLGLKHSRILEKTRQDWLAVDKVLPIRDPNSPRVPNTLFHGNLSEKKHLFRLHWLLP
                     EQKFEVLKENNHWCFLSKDVKIIFSANTIQLNLTIIRAGQQIFPTSSSAESSDPFRDI
                     CGWYSPTYGIHIPALSLILTAETAAPFVICSRWKFLPPGL"
     gene            3133..4341
                     /locus_tag="PKV59_03910"
     CDS             3133..4341
                     /locus_tag="PKV59_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP45930.1"
                     /translation="MHILLIHQAFVTVKEAGGTRHAELAHWLIQKGHQVTVITSPVSY
                     LTGENQPDIRKKQIDRIDGIDVYYCYTSGGLHKGFFPRVMSFFSFMFSSIKTAKKVQN
                     IDIVWGTSPNLFQAFAAWVIAKQQKKKFLLEIRDLWPDFAVGMGVLKNRFLIQASLWL
                     EKFLYAHADRIIVNSPGFIPHIQKISGKTALLVPNGADPDLFVSTDGGFSFRREHQLE
                     EKFVVMYTGAHGPANDLFTVLKAAELLREDDRIRIVLVGSGKEKDLLIKQKEEKGLNN
                     VLFVPPVPKEKIADVIAAADAGLAVLKAIPLFTTTYPNKVFDFMAAGKPVLCQIDGVI
                     RDLVEQYDAGLFVKPGDPQALKEAILLLAGNPEDCRRMGQNGIRTIREHFSRKRIAED
                     FERILFDLLI"
BASE COUNT         1181 a         1015 c          982 g         1201 t
ORIGIN      
        1 accttatctc gggattctga ttgaatatcc gttccaaaat gatgagacaa tttttacaaa
       61 aaatactgtt caactgcggg aaccgtctct tcctcaaccc gttaatctgg gcgtcatcgg
      121 tgccggtaat tatgccaatg ccacttttct tccgattttg aaaagcgcgg ggaaatcgat
      181 ccggcttgtc actatcgttt ccaatcgagg tgccagcgcc cgtgaggcag ccgagaaatt
      241 cggattcgaa aaagcgggtg tcaatgccga aactgtcctt aatgatcccg acataaacac
      301 ggttgtcatt ctgacacgtc acaatcttca tagttccatg gtcatcgaag cgcttaaccg
      361 tcaaaaaaac gtctattgtg aaaaaccgct ggcacttgat tttgattcct tacaggctgt
      421 ttatcaagcc agacaagcca acccgaagcc gattttgatg gtaggttaca atcgtcggtt
      481 tgccccgttg gctgttaaat taaaaaattt cttggctaac tgcgcggaac cccttgccat
      541 gcactatacc gtcaatgccg ggtttatccc gctcactcat tggacacagg atatagaaat
      601 aggcggaggt cggattctcg gcgaatgctg ccatatgctt gactttatgc tctttttaac
      661 ccaatcacga ccgctgcagg tcaatacgat tgctttgccc aataacggac gttatcagga
      721 tgataatgtc agcatccaag taaagtttga gaacggttct atcgggaccg tggcatatct
      781 ggcgaacggc gataaaagct atgcgaaaga acgtttggag gtctttggcg gcggaaaaat
      841 cgcgatccta aatgatttta aaacgctcga aacttgggat cgcgggaacc atcatcgcac
      901 caattcaccg ctgcgaatcg ataaaggaca tgcggcttcc tggcaggctt ttgtctcggc
      961 aatcataaac ggggagcatg aaccgattcc cacggaacag ctcttttatt catcattgac
     1021 ttcgctcgcc gcagtggatt cgttgcacag caatgagatt gtggcagttc ccgatctgaa
     1081 tatctttctc tgattatgaa acagtttgaa gctgattgat aagacaaaac caaatgatga
     1141 atccgatctc aaaaaaatta aaagcgctcc ggtcatttgg aataaaacgt ctctggtatt
     1201 acggaacata tacattaaag gtgaaaagcg gtttttttca aaagcgatct attccgaaaa
     1261 acagtaaaaa taacaaaaaa accgttgctt tctctcctct tccgaacggt cgaaaagatg
     1321 aaatcctgcc ttttcttctt gcagattcga aaagcattct ggaatctgca ggcgaaatta
     1381 tcacggtagg ctacaggccg tattccggca atatcattcg gtctttggat ctggttcctc
     1441 cgcataagga tcttttttgg acccggatac cgcctcgaga aaatttagat atcaaggata
     1501 tttgggaacc ggcaagattt tgttgggtga ccgcactttg cagagcttat gtcttgacag
     1561 gtgatcgaat ttaccctgaa tttttttatc gacaggttca gactttcatt gaatcgaatc
     1621 ctccgtatta cggtgagaat tgggtatcag cccaggaagc agctatccgc atgatttcgg
     1681 ttactttcgc ggcgggtatt ttacagccct cgcttccgga caaaaattca accttcccgg
     1741 atttgccggc ttttattgcg ttacacgcaa cgcggatcat gcaaacccgc agttatgcaa
     1801 aggcacaaaa taacaaccat cgattaagtg aggcagccgg tttgatgacc gccgcttgcg
     1861 ttcttccgaa atcaccggat gcttcaaaat ggttctcgac cggatggaaa gattttcaaa
     1921 atgcactgaa ttctcaaatc cttcccgacg gtgcctacat tcaatacagt gtcaattatc
     1981 accggttaat gctgcagctt gttctttggg tggattcatt gttgaagacc caaaaaattg
     2041 cttggcccgc aaatttgatt cggaagattc gtcgatcggt gctttggctg gcaaaacata
     2101 cggatgaatt cagcggccaa gcttgtaata ccggccacaa tgacggatct ctgctttttg
     2161 ctctgggcgg aaactataac gactttcgcc ccaccttgca aaccgctgcc gtcgtctttc
     2221 tcggaaaacc tcttttcccg cccggaccgt gggatgaaat gcgaatttgg ctcggttgtt
     2281 ataatcgcgc gggcgtcgat ccggattctc aattcgaatc cactttttat tcacaaaacg
     2341 ttcaaaggat ttctgcaggg aatcaatggg gaattctgca tacagccggt ttcaaagacc
     2401 gacctgccca tgcggatcaa ttgcatgttt ctctttggaa gggcggtaaa tgtatggctt
     2461 tagatgccgg tacctaccgt tataccgctg ctccgccatg gaataatcga ttaaaatccg
     2521 cgttcgttca taattgtctg acgattgatt gtcaggaacc gatgatcgac gccggaaaat
     2581 ttttatggct ggattgggat caggcacgga ttctggatca aacggaattt cgaaaaatcg
     2641 ccgagcacag cggctataaa cgattaggat taaaacattc acgaattctc gaaaaaaccc
     2701 gacaagattg gttggcggtg gataaggtct taccgatccg agacccgaat tcgccacgag
     2761 tcccaaacac tttgtttcat gggaatcttt cggaaaagaa acatctgttc cgcctgcact
     2821 ggctgctgcc tgaacaaaaa tttgaggttc tgaaagagaa taaccattgg tgttttcttt
     2881 cgaaagatgt aaaaataatt ttttccgcca atacaattca actgaatcta acaattattc
     2941 gagcgggtca acaaatattc ccgaccagtt cttccgccga aagttccgat ccatttcgtg
     3001 acatctgcgg ttggtactct cccacctacg gaattcatat tcccgccctt tctctgattt
     3061 taaccgcgga gaccgctgcc cctttcgtta tctgctcgcg ttggaaattc ttaccaccgg
     3121 ggttatgact gtatgcatat ccttttgatt catcaagcat tcgtgacggt taaagaggcg
     3181 ggaggaaccc gccatgcgga attggctcac tggttgattc aaaaaggtca tcaagtgact
     3241 gtaatcacga gtcccgtcag ctatttaaca ggagaaaacc aaccggatat ccgaaagaag
     3301 caaatcgacc ggatagacgg aattgatgtc tattattgct atacaagcgg aggattacat
     3361 aaaggttttt tcccgcgggt aatgtctttc ttcagcttca tgttttcatc gataaagacc
     3421 gcaaaaaagg tgcaaaatat tgatatcgtt tggggaactt ctcccaatct gtttcaggca
     3481 tttgccgcat gggtaattgc gaaacaacag aaaaagaaat ttttgttgga aattcgagat
     3541 ttatggcccg attttgccgt cggaatgggt gtattaaaga acagattcct tattcaagct
     3601 tcgctttggc ttgaaaagtt tttatacgca catgctgacc ggatcatcgt caattcaccc
     3661 ggatttattc cccatattca aaaaatcagc ggaaaaacag cccttctggt tcctaacggc
     3721 gctgatcccg atctgtttgt ttcgaccgat ggcggctttt cgttccgacg ggagcatcaa
     3781 cttgaagaga aattcgtcgt aatgtatacg ggagcacacg gacccgccaa cgatctattc
     3841 acggtattga aagcggcgga attgcttcga gaggatgatc ggattcgcat cgttttagtc
     3901 ggcagcggga aagaaaagga tcttctgatc aaacaaaaag aagaaaaagg gttgaataat
     3961 gtattgttcg ttcctcccgt ccccaaggaa aagatcgcgg atgtcatcgc cgctgccgat
     4021 gccggtttag cggttttaaa agccattccg ctttttacca ccacctaccc caataaggtc
     4081 tttgacttta tggcggccgg caagccggtg ctttgccaaa tcgatggcgt tatccgagat
     4141 ttggtcgagc aatacgatgc cggtcttttt gtcaagccag gagatcccca agctctgaaa
     4201 gaggcaattc ttttacttgc cggcaatccg gaagactgta gacgcatggg gcaaaacggc
     4261 atccggacga ttcgggaaca tttttcccgt aaaagaatcg cggaagattt cgaacggatt
     4321 ctattcgatc ttttaatata atgatttatt ttctatccgt cataccttcc atcccccat
//
LOCUS       DAOMIV010000119         4364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20720_length_4364_cov_4.477141, whole genome shotgun sequence.
ACCESSION   DAOMIV010000119 DAOMIV010000000
VERSION     DAOMIV010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20720_length_4364_cov_4.477141"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..712
                     /locus_tag="PKV59_03915"
     CDS             146..712
                     /locus_tag="PKV59_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQP45931.1"
                     /translation="MHAADLSPHQLDASVTVVGTGMVGLLVIQALRAYGYGKIIAVDV
                     DSKRLELAKQMGADDTVISDENALEKIRMLSGGEGTDAAFEVVGADATFNLAVNSVHK
                     GGSVTLVGNISQKTNFPLQSVVTRQIRLQGSCASSGEYPACLNMIARGSFNVNPIISA
                     TAPLSEGPEWFDNLYYQKVNALKVILNP"
     gene            758..3991
                     /locus_tag="PKV59_03920"
     CDS             758..3991
                     /locus_tag="PKV59_03920"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HQP45932.1"
                     /translation="MNKTVVIASDHAGFELKQNIIETVEEAGCKVIDVGAFSKESSDF
                     PDFVQLGAEKIKSGKADAGIFLCGSGVGVCIAANKIPGIYASVCHDTYSAHQGVEHDG
                     MNVLCLGARIIGPELANELVKAFLGAAFNNKPNQIRRFNKIKQIEAGDLYLINRIEAL
                     RDIGQSVWLDQANRCQLKNGELVKMIEKGQVSGLIMNSGNMCSAILNSDDYASALVPM
                     MLAGWNCRKIFDKLLVDDVRSAAGIFKDLYISSGGKEGYVTLTLDPELSHNIEKTVSE
                     TKRIWTAVNRPNILIGIPATEEGLEAAKQLVTEGININFTMIVSEKSYSGAVDAYLSG
                     LETRLKEGKPIDKIYSTASVDVAEIDHIVNKFLDQAVDPVDPDKSTATRGLKMKAALI
                     ICRRIFNLFLETNQSGRMQNLIAEGAEIQHLLWSSLCHKEPSFKDTYYTDSLIAENTI
                     LSVHPKLMDAVIDHGQIIPSLRVPHEEDNQIIEEFEKLGIHFESLTERVQEEIMESYK
                     SAFESMLKELDKKSDRVKKSLGCLYTPVFENFKKLEEDSIIQRIFAKDPTVWTFDTQA
                     YPEIRNRLGWLDAHQNIAKSIDEYKSIAAGLKKDGIHKILLMGMGGSSLAPEVMALTF
                     KNNDGMALEIIDSTDPVQVLAADASHPPKETVYIVSSKSGGTAEVQAFLDYFFERAKT
                     ELADQAGSHFIAITDPGTQLERKAKEMGFRNIILADPSIGGRFSALSAFGVFPAVLMG
                     INPETIIEQATRMAKICAPSNSVGCNEGAALGVYMGTAALNGRDKFTILTDPEFSAFG
                     SWLEQLIAESTGKMGKGIVPIDIEPKLAPSEYAEDRAFVYVETSGTQTDFVNELIAAG
                     QPVLTIKLNDLNELFAEFYRWEIAVAVACSLLGVNAFDQPNVQDSKNRTVAKITEYKE
                     KGQLTDFPACWEKDGLSVYFNTDIPDLKQADDIKYLIKAFIRQAIKGKDYIAINAYLP
                     RNEKMSADLQNVRAWILKETQCATTLGFGPRFQHSTGQLHKGGANNGLFIQLIADASQ
                     DIEIPNEGITFSILEKAQALGDFEALLAKDRRAIRINLGSKAVTDLL"
     gene            4140..>4364
                     /locus_tag="PKV59_03925"
     CDS             4140..>4364
                     /locus_tag="PKV59_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45933.1"
                     /translation="MKHQTRLCINPDCTLRFTIPLSENISIRCPRCGSGTIPIDEGYD
                     SNLIPQRKQDESELPTVVAVLDNIRSALNVG"
BASE COUNT         1258 a          912 c         1042 g         1152 t
ORIGIN      
        1 tcgtgtttta ggcgtgtcct gtgacgaata tcgtcgggac ggtgcgttcg ccgaataggt
       61 tcccgttcct cagcacatac tctatgcgtt accggatgaa gtcgatttta atcaagccgc
      121 cctcgtagaa ccgctttccg tcgccgtgca tgcggcagat ctaagtccgc atcagctcga
      181 tgcatcggta acggtagtcg gaacgggaat ggtcggttta ttggtgattc aagctttacg
      241 agcgtatggc tacggaaaaa tcatcgccgt tgacgttgat tccaaacgat tggaattggc
      301 aaaacagatg ggcgcggatg atacggtaat ttccgatgag aacgcactcg agaaaattcg
      361 catgttatcc ggaggagaag gaacggatgc cgcttttgaa gtggtcggtg ctgatgcaac
      421 gttcaacctg gccgtcaata gtgtgcataa aggaggttca gtcacccttg taggaaatat
      481 ttcgcagaaa acgaactttc cgcttcaatc cgttgttacc cgacaaatcc gattgcaagg
      541 gtcctgtgca tcttccggag aatatccggc atgtttgaat atgattgccc gaggttcttt
      601 taatgtgaat ccgattatca gcgctacagc tccgttatca gagggtccgg aatggtttga
      661 taatctttat tatcaaaaag tgaacgcatt gaaagtgatt ttgaatccgt aacaaaattg
      721 aaagataggt ttttttgtca gataatggga gattcctttg aataaaacag tagtaattgc
      781 cagcgatcat gccggatttg agctgaaaca aaatattatt gaaacagtcg aggaagccgg
      841 ctgtaaagtg atcgatgtgg gagcattttc caaagaaagt tcggatttcc ccgatttcgt
      901 tcaactggga gccgaaaaaa ttaaatcggg gaaagcagac gccggcattt ttctctgcgg
      961 atcgggagta ggtgtttgta tcgccgccaa taaaatcccg gggatctatg catcagtttg
     1021 ccatgatacc tattctgctc atcagggagt ggaacatgac ggaatgaatg tcttgtgtct
     1081 cggcgccaga atcatcggac ccgaattggc aaacgagttg gttaaagcat ttctcggtgc
     1141 cgctttcaat aataagccga atcaaatcag gcggttcaat aaaatcaaac aaatcgaagc
     1201 cggagacctt tacctgatca accgaatcga agcattacgg gatataggac aatctgtctg
     1261 gttggatcag gctaatcgct gccaactgaa aaacggcgaa ttggtcaaaa tgatcgaaaa
     1321 aggtcaggta agcggtttaa taatgaattc cggtaatatg tgcagcgcaa tcttgaacag
     1381 cgatgactat gcgtcggctt tggttcccat gatgcttgcc ggctggaatt gccgcaaaat
     1441 cttcgataaa ctcttggttg atgatgttcg gtccgctgcg gggattttta aggatcttta
     1501 tatcagcagc ggcggaaaag aaggatatgt cactctaacc ctggatcctg aattgtcaca
     1561 taatatcgaa aaaaccgtgt ccgaaacgaa acggatttgg actgcggtga atcggcctaa
     1621 tattttgatt ggcattccgg ccaccgaaga aggtttggaa gccgcaaaac agttggtaac
     1681 ggaagggatc aatatcaatt ttacaatgat tgtttccgaa aaaagctatt cgggtgccgt
     1741 tgacgcctat ctttccggtt tggaaacgcg attaaaagaa gggaaaccta tcgataaaat
     1801 ttattccaca gcttctgtcg acgttgcgga aatcgatcat atcgtcaaca aatttttgga
     1861 tcaggctgtc gatcctgtcg atccggataa gtcaaccgct acccgtggat tgaaaatgaa
     1921 agccgcattg atcatttgcc gccgaatctt caatcttttc ctggaaacca atcaaagcgg
     1981 cagaatgcaa aatctaattg ctgaaggcgc tgaaatacaa catcttttat ggtcatctct
     2041 ctgccataag gagccttcat tcaaagacac ttattatacg gattcgttaa tcgcagaaaa
     2101 tacgatccta tccgttcatc cgaagttaat ggacgccgtt atcgatcatg gacagatcat
     2161 accatccctt cgagtaccgc atgaggaaga taaccaaatt attgaagaat ttgaaaagct
     2221 cggtattcat ttcgaatcgc ttacggaacg tgttcaggag gaaatcatgg aatcttataa
     2281 aagtgccttt gaatcaatgt taaaagaatt agacaaaaag tcggatcggg tgaaaaaatc
     2341 attgggatgc ctttacactc cagtctttga gaattttaag aaactcgaag aagattcgat
     2401 tatccagcga atattcgcaa aagaccccac cgtttggaca ttcgatacac aagcctatcc
     2461 cgaaattcgg aatcgattgg gatggttgga tgcgcatcaa aacattgcca aatcaatcga
     2521 cgaatataaa tcaatcgctg ccggcttgaa aaaggacggc atccataaga ttttgctgat
     2581 ggggatggga ggatcttcgc tggctcccga agtaatggca ttaaccttca aaaataacga
     2641 tgggatggca ttggaaataa tcgattccac cgatccggtt caagtcttgg cggccgacgc
     2701 atctcatcct cccaaagaaa cggtctatat cgtttccagc aaatcgggag ggacggcaga
     2761 agttcaggca tttttggatt atttcttcga aagagccaaa acagaattgg ctgatcaggc
     2821 cggatcccat ttcattgcca ttactgatcc cggtactcag ctggaacgca aagccaaaga
     2881 aatgggattt cggaatatca ttttggcgga tccctccatc ggaggacgat tctcggcatt
     2941 atccgcattc ggagtttttc cggctgtttt gatgggaatc aatccggaaa caattatcga
     3001 acaggctacc cgcatggcaa aaatttgcgc tccttccaat tctgtcggat gtaatgaggg
     3061 cgctgctctg ggcgtttata tgggcacggc tgccctgaac ggcagagata aattcactat
     3121 tttgaccgat ccggaatttt cagctttcgg ctcctggttg gaacagctga tcgccgaaag
     3181 caccgggaaa atgggcaaag gaattgttcc gatcgatatc gaaccgaaac tggctccttc
     3241 tgaatatgcg gaagatcgag catttgttta cgtggagact tccggtacac aaacagactt
     3301 tgtcaatgaa ttgatcgcag ccggtcaacc ggttctcacg ataaaattga atgatttgaa
     3361 cgaattattt gccgagtttt atcgttggga aatcgccgtt gcagtagctt gctcgctgtt
     3421 gggtgtgaat gcgtttgatc aaccgaatgt gcaggacagt aaaaatcgta cagtcgccaa
     3481 gattacggaa tataaagaaa aagggcaact gacagatttt cccgcttgct gggaaaaaga
     3541 cggcctcagc gtttatttca atacggatat accggatttg aaacaggcgg atgatataaa
     3601 gtatctgata aaggctttca ttcggcaggc gataaaaggg aaagattaca ttgcaatcaa
     3661 tgcttattta cccagaaatg aaaagatgtc ggcagatttg caaaatgttc gggcttggat
     3721 tttgaaagaa actcaatgtg ccactaccct cggtttcggt ccccgttttc agcactccac
     3781 cggacaatta cataaagggg gtgcgaataa cggtctgttt atccaactga tcgcggacgc
     3841 gtcgcaagat atcgaaattc ccaatgaagg gattactttt tcaatcttgg aaaaagctca
     3901 ggctttaggt gatttcgaag ctctgttggc gaaagatcgt cgagccatcc gaatcaattt
     3961 gggatccaaa gccgtaacgg atctcttata ggaaagagaa ttgtaaaaag gcggaggaac
     4021 ctctttagat cctctgcctt ttaaccttta taaccgttat ttgtctattc gatcaacgat
     4081 ccgaataaga atgatcgaaa acggaatgaa attctgtctt gactttatga atcaaagcga
     4141 tgaaacacca aacacgcctc tgcattaatc ccgattgtac tttacgcttt acgattccgc
     4201 tttcagagaa tatttcgatc cgctgtccgc gatgcggcag cgggacgatt ccgatagatg
     4261 agggttacga ttcgaacttg ataccccaac gaaagcaaga tgaatccgag ttgccgactg
     4321 tggtcgcggt tttagataat attcgaagtg ctctcaatgt cgga
//
LOCUS       DAOMIV010000120         4344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20867_length_4344_cov_4.943810, whole genome shotgun sequence.
ACCESSION   DAOMIV010000120 DAOMIV010000000
VERSION     DAOMIV010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20867_length_4344_cov_4.943810"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PKV59_03930"
     CDS             <1..828
                     /locus_tag="PKV59_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45934.1"
                     /translation="QYVCDFCPVVSSAQITQPEPGFNRLTVDSGINVYIENPEGQKTG
                     YDWKTGKTYAEIPDLEINRTMRQTSAQLPVNFPYYLWLNAPDLHSWATFDVSFTSQGT
                     VLYLQNVLESYEYPNIIYKPGDSKQEGNSNTETFEVVAFPDRLPTVQFVISNEQGECR
                     FDLNILFKGMVDPNLKVDIQIFHNPKTGRFGINIYPAKDAETEQYLQTTFTISGDFYL
                     YTDQDEKHIAINNETPIEMRVNGNISIDYQAWQKNGTFFINGDLDGDSIVEIAKEFQ"
     gene            1112..1465
                     /locus_tag="PKV59_03935"
     CDS             1112..1465
                     /locus_tag="PKV59_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQP45935.1"
                     /translation="MPIYSYQCDNCDYEFDIFQHFSDEALTECPKCHKNTLRKLYTPV
                     GIVFKGSGFYATDHRSPSGMMPSPHEHEAKESSGSESAETEGSKSVESSQSVEKPKSE
                     SHVSKTKEKAEVKRG"
     gene            1500..1718
                     /locus_tag="PKV59_03940"
     CDS             1500..1718
                     /locus_tag="PKV59_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45936.1"
                     /translation="MAGEKDGKENNKAGKSGTGKESAECRKEEAQQNDKHLRDCDCCF
                     VDFLDDHNDDSILASEHYQSELINLIRF"
     gene            1797..2828
                     /locus_tag="PKV59_03945"
     CDS             1797..2828
                     /locus_tag="PKV59_03945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQP45937.1"
                     /translation="MKSTGTNLLESDRFFEEFQKAVAYRRKLVLSDPLRCCRLFNGFY
                     EGNPAFSLDLFAATLVIQSYSEGTVSPENWLAFIQKAVNPMIPGIKNVLLKEHFSDDI
                     EKRNGTILSGGPLDERIREWGVEYAIDLQLNRDSGFYLDNALLRKWLIDHCEGKRVLN
                     TFSYTGSLGMAALAGGAIRIVQTDLNEKFLNQFHKTAEINGVSAERYKNIAGDFFKIM
                     ALMRKEEQLFDIVILDPPFYSKTRNGIVDLQKNFINLINKIRPLVADGGKIIAINNAL
                     YVSGSEMMDRLEEAFDDGYIQIDERIPVPESFYGGDLSRAKLPADPFPFDHPTKIVTL
                     NVKRKDGRR"
     gene            2840..4192
                     /locus_tag="PKV59_03950"
     CDS             2840..4192
                     /locus_tag="PKV59_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45938.1"
                     /translation="MQKRKFRNISVSTILLFGIFFLIGCKVQVYDLQPVIATGTPVLS
                     AAKESQTLVMRNTSGPLPAVTNTPPIHIAPTRISESVPIEILELVMINGGSGWGIGKV
                     ANGQDKIIVKTTNGGANWKNVSSSQAVYENAGKNKEISAWFLDSNQAWVVYWDNVNWS
                     STNQIPVWKTTDSGGNWEKSILPTEGYSLQYFKDVQIRFIDPQIGWVFAKMGKTEERE
                     YIGLFTTHDGGKSWNTMVSPDSVNLPSKGGKNGAVFRNATEGWISGSNAKDDPGSLLW
                     HSIDGGNTWNKQVLPQYLAEGIPSDLLSNRQYSCSLTPPKFVDYLYQYAWMKMVCQGG
                     ELAEPLGLLYWTYDGGNSWRVYQLPAAEGSLVFYGIYQGWYSKTAAPGSSYPYEILGT
                     SNGGENWNLIAQTAWKSNLQFITASIGWGVVEYEGHYAMVKTGDGGFTWEQIFPVVDP
                     "
BASE COUNT         1327 a          805 c         1033 g         1179 t
ORIGIN      
        1 caatatgtct gtgatttttg tccggtcgtt tcatccgcac aaatcaccca gcccgagccg
       61 ggattcaatc gtttaacagt tgattctgga attaatgtgt atattgaaaa tccggaaggg
      121 caaaaaacgg gatacgactg gaagaccgga aaaacgtatg cggagattcc cgatttggag
      181 atcaaccgga caatgcggca gacttcagcc caattaccgg taaattttcc ttactatctg
      241 tggttgaatg cccctgatct gcatagttgg gcaacgtttg atgtcagctt tacttcacaa
      301 ggaaccgttc tctatttaca gaatgtattg gaatcttatg agtatccgaa tatcatttac
      361 aagccgggag attctaaaca ggaggggaat tccaataccg agactttcga ggtggtagct
      421 tttccggacc gtttaccgac tgttcaattc gtgatcagta atgaacaagg agaatgtcgt
      481 tttgatttga atattttatt taaagggatg gtcgacccca atttgaaagt tgatattcaa
      541 atttttcata atcccaaaac aggtcgattt gggatcaata tttatccggc aaaagatgcc
      601 gaaacggaac agtatctgca aactactttt acgatcagcg gcgattttta tctttacaca
      661 gatcaagacg aaaaacatat tgccatcaat aatgaaacgc cgatcgaaat gcgggtaaac
      721 gggaacattt ccatcgatta tcaagcttgg caaaagaacg ggacgttttt cataaacggt
      781 gatttggacg gagattcaat cgttgaaatc gccaaagagt ttcaataaat cgagtatatt
      841 tctgacagaa tattatccgg aatatttgca tacctcattc tcggtctgag cgaaaccgtt
      901 gatgaaacat ctaaaatgaa ggaatatgta ttagagaaat atcagagatc gtccggacga
      961 taaaacatgt cgtaaaatta aacgaaaatt gataatttga tataatagaa cgcgtattgg
     1021 atttttttat cgtctatgct gattcaaaaa tacaaaaaaa agataaaagg ataaattaat
     1081 ttcaatacga ttgaatgatc gaggtttgaa aatgccaatt tattcttatc aatgtgataa
     1141 ttgtgattat gagtttgata tctttcaaca tttttcggat gaagctttaa ccgaatgtcc
     1201 gaaatgtcat aaaaatacct tgcggaagct ttatacaccg gtagggatcg tgtttaaggg
     1261 atcggggttt tacgctaccg atcaccgttc accatccgga atgatgcctt caccgcacga
     1321 acatgaagcg aaagaaagct ccggatccga atccgccgaa acggaaggtt cgaaatccgt
     1381 tgaaagctct caatcggttg aaaaaccgaa atcggaatca cacgtgagca aaacgaaaga
     1441 aaaagcggaa gtcaaaagag gataatccga ggaagctgag atcgaaaaaa tgcttccggg
     1501 tggcaggtga aaaagatggc aaagaaaata acaaagcagg aaagagcggt acaggaaaag
     1561 aatcggcaga atgccgcaaa gaagaagcgc aacagaacga taagcatctt agggattgtg
     1621 attgctgttt tgttgatttt ctcgatgatc ataacgacga ttcgatttta gcttctgagc
     1681 attatcaatc cgaattgata aacctcattc gattttgaat gaggtttttg tggttaaatt
     1741 tttgagataa caaaaaaatc tatagaatat aaagattttt tgagagagat gataggatga
     1801 aatcaacggg aacgaatttg ttggaatcag atcgcttttt tgaggagttt caaaaagcag
     1861 tggcttatcg ccggaaattg gttttatccg atcccctgcg gtgctgtcgc ctgtttaatg
     1921 gattctatga gggaaatccg gctttcagcc ttgatttatt tgcagctaca ctggtaattc
     1981 aatcttattc cgaaggaact gtttcgcccg aaaattggct ggctttcatc caaaaagcgg
     2041 tgaatccgat gattcccgga ataaaaaacg tcctactgaa agaacatttt tctgacgata
     2101 tcgaaaaaag aaacggcacg attttatccg gcggaccttt ggatgagcga attcgggaat
     2161 ggggtgtgga atatgcgatc gatttacaat tgaacagaga cagcgggttt tatttggata
     2221 acgcattatt gcggaaatgg ttaatcgatc attgcgaagg gaaacgggta ttgaatacat
     2281 tttcttatac cggaagcctt gggatggcag ctttagccgg cggagcaatt cgcatcgttc
     2341 aaacggatct gaatgagaaa tttcttaatc aatttcataa gacagccgag atcaacggag
     2401 tttccgccga aagatataaa aacatagccg gtgatttttt caaaattatg gctcttatgc
     2461 gaaaagaaga gcaacttttt gatattgtaa ttttggatcc gccattctat tcaaaaacac
     2521 gaaacggcat tgttgattta caaaaaaatt ttatcaacct tattaacaag attcgccctt
     2581 tagtggcgga tggaggtaag atcatcgcta tcaacaatgc actttatgtt tcgggctcgg
     2641 agatgatgga ccggctggaa gaagcttttg atgacgggta tatccaaatc gatgagagaa
     2701 tcccggtacc ggaatctttt tatggcggag atttatcccg ggcaaaattg cctgcggatc
     2761 cttttccttt cgatcacccg acgaaaatag ttaccttgaa tgtcaagcgg aaggatggca
     2821 gaagataaaa ggagtgctta tgcagaaaag aaaatttcgg aatatttctg tttctacgat
     2881 tctgcttttc ggcatcttct ttctgatcgg ttgcaaagtt caagtttatg accttcaacc
     2941 ggttatcgcg accggtacac cggtactttc cgcagcgaaa gaaagccaga cacttgtcat
     3001 gcgaaatact tccggacctc ttcccgccgt aaccaacacc ccgccgattc atatcgctcc
     3061 gacgcgaatc agtgaaagtg ttccgatcga gatattagaa ttagtgatga tcaacggagg
     3121 gagcgggtgg ggaatcggca aggttgcgaa cggccaagat aagatcatag tgaaaacaac
     3181 caacggcggc gcaaattgga agaatgtatc ttcttctcaa gcggtttatg agaatgccgg
     3241 taaaaataaa gagatatcag cctggttctt ggattcaaat caggcctggg tggtttattg
     3301 ggacaatgta aattggtctt ctaccaatca gattcccgta tggaaaacga ccgatagcgg
     3361 tggaaattgg gaaaaatcga ttctgccgac agaaggatat tccctgcaat attttaagga
     3421 tgtacaaatc cgttttatcg atccacaaat aggttgggtt ttcgccaaaa tggggaaaac
     3481 cgaagaacgg gaatatatcg gattattcac tacccatgat ggcgggaaaa gttggaacac
     3541 gatggtgtcg ccggattccg taaacttacc ttccaaagga ggaaaaaacg gagctgtatt
     3601 ccgcaatgcg acagaaggat ggatcagtgg cagcaatgcc aaagatgatc ctggcagttt
     3661 attatggcat agcattgacg gcggtaacac gtggaataag caggttttac cacaatatct
     3721 tgccgaaggc attccatcgg atctgttaag caatcgtcaa tattcatgca gcttgacgcc
     3781 gccgaaattt gtggattatc tttatcagta tgcctggatg aaaatggtct gtcagggagg
     3841 tgagctggcc gagcctcttg ggctgcttta ttggacctac gacggtggaa attcctggcg
     3901 ggtttatcaa ttgcccgctg cagagggatc attggttttt tatggaattt atcaggggtg
     3961 gtattcaaaa acggcagcac ccggttcaag ctatccctat gaaattttgg gaacgtcaaa
     4021 cgggggagaa aattggaatc tcattgcgca gacagcctgg aagagcaatt tgcagtttat
     4081 taccgcttcc atcggatggg gcgtcgtcga atatgaagga cattatgcta tggtgaaaac
     4141 cggcgatgga ggattcacct gggaacaaat ttttccggtt gtcgatccgt aaatcggttt
     4201 gcgtggatag gtagaattgc aggtagatag ggggatttgg tttttcatcc tgtgtagggg
     4261 acggcgtcct cgacgtcccg tggttgcgga aataatgtag gggtcggttc caaaccgacc
     4321 cttttttgta accatgcccg aaag
//
LOCUS       DAOMIV010000121         4344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20869_length_4344_cov_3.956400, whole genome shotgun sequence.
ACCESSION   DAOMIV010000121 DAOMIV010000000
VERSION     DAOMIV010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20869_length_4344_cov_3.956400"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PKV59_03955"
     CDS             complement(<1..1000)
                     /locus_tag="PKV59_03955"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQP45939.1"
                     /translation="MLVEMKGIVKTFGPVVAVDHVDFTVYPREIRGLLGENGAGKTTL
                     MNVLAGTFPPTEGEIYIDGKKVQFSNSLQAIKMGIRFIHQEISLCNDLKVFENMFLAE
                     EILLPNGLLNKKEMIKRASEVFNRMQVNINPEELVWNLQPAEKQLVEIARALLFKSDL
                     IIMDEPSTALTTKDIERLFVIMRQLKSEGVSFIYISHKMPELFEVCDTFSVMRDGKLV
                     AEGNFSDVNEQEITELMIGRQLAEEEFSHKPCYRTDEIALSVRNLSGPGFQNISFDLH
                     RGEIIAITGLQGSGRDSLADALFGAIPSSGEIEIFGKKINPESGIIERMKSGIAMVP"
     gene            complement(1085..2140)
                     /locus_tag="PKV59_03960"
     CDS             complement(1085..2140)
                     /locus_tag="PKV59_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQP45940.1"
                     /translation="MSKKLFVLSVVFVLVLALTGSVLAADTFKIGVVTPDADHGFTGE
                     SVAHARAELEAKSKELGFEYKFEVGGEAAKQIAALETILEWKPDVIVLWPLEGQQLKN
                     IAMTIMDSGVKLIIYDRLIDGFKPTAEIMGDNESIGKMMGEYLLKYFDEQLKVGETIT
                     YLRFIGDSSTVSIQRSKGMDDVIAASAYKDQFKQIQENYQTDWSNAKAQEQMENWLNT
                     ADPKEIEDLDLIVTHDDEVVDGVVVALNNYYNSNPSAKLNVKLITGVGGRRETLATFD
                     KPVANGIDLVTYYFSPSFIREAIRLGIAAAKGEQYQGQDINGQLFLIPSIEIDKTNVD
                     EFRNSEAFKERYSISLN"
     gene            complement(2194..3225)
                     /locus_tag="PKV59_03965"
     CDS             complement(2194..3225)
                     /locus_tag="PKV59_03965"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP45941.1"
                     /translation="MTVTIFDVAKEANVSASTVSRVLNNKSTISENTVRRVYEAAERL
                     GYEPNILAQSFRKQETRTILILTPNMTNPYYSNILSGISDTTRSLFYSSFICKTDWNR
                     EEMSNILGMMKKRHADGAILLACEMGEEDWLLRYAKDNPIILCSEFNPDTSIPHISID
                     NYKATVEAIEYLISLGHTKIGTISSVNKYLSTQLRLKGYKDALAAAGIEFHPEYVEYA
                     SKDYSYRSGCASARKLLSLEDRPTAIFCISDVLAIGAINTAAEMGIKVPEDLSVIGFD
                     DVEQTAMFSPKLTTVAQPCYELGKKSAELLITAFNQSEDVPLETVLPYQLILRESTGS
                     PVKIKEESI"
     gene            3481..>4344
                     /locus_tag="PKV59_03970"
     CDS             3481..>4344
                     /locus_tag="PKV59_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQP45942.1"
                     /translation="MSFVHLHLHTEYSLLDGFTKINKLMTRVKEMNMPAVAITDHGTM
                     FGVVEFFFAAKEAGIKPIIGLEGYLSKGSMTSRDPKEKTSSHLLLLAKDMTGYKNLLQ
                     IASASQLEGYYYHPRIDHEYLAEHAEGLIATSGCMAAELPRMIINGESEAAKSQLDWY
                     LQVFGRENFYIELQDHDVPEIKKLNNVLLRLGKEYDIPFVATNDTHYVDPEDWKYQDI
                     MLAIQTGAKLTDSNRFRMSDKSYYLRSPEEMEKLFGYIPGAIENTLAIAERCNVDLSS
                     KGHHLPIFPLET"
BASE COUNT         1166 a          990 c          951 g         1237 t
ORIGIN      
        1 gcggcaccat ggctatgccc gacttcattc gctcgatgat tccgctttcc ggattgatct
       61 ttttaccaaa gatctcgatt tctcccgaag aagggatcgc gccgaaaagc gcatcggcta
      121 aagaatctcg tccggatccc tgcaaacctg tgatggcgat aatctctcct ctatgcaaat
      181 cgaaactgat attttgaaag cccggaccag aaagatttcg gactgaaagg gcaatttcgt
      241 ccgttcgata acaaggttta tgagagaatt cttcttcggc gagttgtcga ccgatcatca
      301 actcggtgat ctcctgctca tttacatccg aaaaattccc ctccgcaacc aattttccgt
      361 ctcgcataac cgaaaaagta tcacatactt caaacagttc aggcatttta tgcgaaatat
      421 aaataaaact gacgccttcc gatttcaact ggcgcataat gacaaacagc cgttcgatat
      481 ccttggtcgt caaagctgta gacggctcat ccataataat caggtcactc ttaaacaaca
      541 gagcacgggc gatttcaacc agttgctttt cggcaggttg caaattccaa acaagttctt
      601 ccggattgat attaacctgc attcggttaa aaacttcgga tgcccttttg atcatttcct
      661 tcttatttaa gaggccgttc ggcagaagaa tttcttctgc caaaaacata ttttcaaaaa
      721 ctttcaaatc gttacagagg ctgatttcct ggtgaataaa acgaattccc atcttaatcg
      781 cttgcagcga attggagaat tggactttct ttccgtcaat atagatctcc ccttcggtcg
      841 gcggaaaagt tcccgcaagc acattcatca gagtcgtttt accggcgccg ttttctccca
      901 acagccctcg tatttccctc ggatagaccg taaaatctac atgatccacg gcgaccaccg
      961 gtccgaaagt ttttacgatt cctttcattt ctacgagcaa ggcaggttct ccttatactt
     1021 tattgaaaaa ctgccggtct aaaaaaaccg gaccggcagt aactgcttga aaagataaaa
     1081 atggttagtt gagtgaaatg ctatagcgtt ccttgaaagc ctcactgttg cggaactcat
     1141 cgacattggt cttgtcaatt tcaatggacg ggatgaggaa gagttgccca tttatatctt
     1201 gtccctgata ttgttcgcct ttagcggcgg caatacctaa gcggatggct tcacgaatga
     1261 aggacgggct gaaataatag gttaccagat cgataccgtt tgcaaccggt ttatcgaagg
     1321 ttgccagcgt ttctcgtctg ccgcctacac ccgtgatgag tttcacgttc agttttgccg
     1381 aaggattgct gttatagtag ttattgaggg caactacgac accgtcgaca acctcatcat
     1441 catgggttac gatcaaatcc agatcttcga tttccttcgg atcagccgtg ttcagccagt
     1501 tttccatctg ttcctgtgct tttgcgtttg accagtcggt ctgatagttt tcctgaatct
     1561 gcttgaattg atctttgtat gcggatgcgg caatcacatc atccataccc ttcgatcgct
     1621 ggatggaaac ggtcgagctg tcaccgataa agcgcaggta ggtgattgtt tcgccgactt
     1681 tgagttgttc gtcgaaatac ttgagcagat attcgcccat catttttccg atactctcat
     1741 tgtcgcccat aatttcggcg gtcggtttga aaccgtcaat cagacgatca tagatgatca
     1801 gtttcacacc ggaatccata atggtcatgg caatgttctt taattgttga ccttccaacg
     1861 gccaaaggac gatgacatcc ggtttccact caagaatggt ctcgagagcg gcaatctgtt
     1921 tagctgcttc gccgccgact tcaaatttat attcgaagcc gagttctttg gactttgctt
     1981 cgagttctgc acgggcatga gcaacgctct cgccggtgaa accatgatcc gcgtccggag
     2041 tgactacacc gatcttgaat gtgtcagctg ccaggacgga acctgttagt gcaagcacta
     2101 agacgaaaac aacggataac acaaaaagct ttttagacat tttttccctc ctaaaagatt
     2161 tactgcgttt ttgtgaacga tttgaagtgc ttttcaaatc gattcctcct taatttttac
     2221 gggacttccg gtcgattctc ttaggattaa ttgatacggg agaaccgttt caagcggaac
     2281 gtcctcggac tgattgaatg ccgtgatcag caattcagcc gattttttcc ccaattcata
     2341 acatggttga gcgactgtgg ttaatttcgg gctgaacatc gccgtttgct cgacgtcatc
     2401 gaacccgata accgataaat cttccggtac tttgataccc atttcggcgg cagtattgat
     2461 ggcaccgatt gccagaacat ccgaaatgca gaatatagcg gtcggccgat cttccaatga
     2521 cagcagcttt ctggcagaag cgcaaccgct tctgtaactg taatccttgg atgcatattc
     2581 gacatattcg ggatggaact caattccggc ggctgccaaa gcatctttgt atcctttcaa
     2641 gcgcagttga gtggacaaat atttgttgac gctgctgatc gttcctattt ttgtatgacc
     2701 gagcgaaatc agatactcga tggcttccac cgtcgctttg taattgtcga tggaaatatg
     2761 aggaatactg gtatccgggt taaattccga acagagaata atcggattat ctttcgcata
     2821 gcgcaaaagc caatcctctt cccccatttc gcatgccaat aaaatagcac cgtccgcgtg
     2881 ccgtttcttc atcattccga gaatattact catttcttcg cgattccaat cggttttgca
     2941 gataaacgag ctgtaaaaaa gggatcgtgt cgtatcactg atgcccgata gaatattgga
     3001 ataataagga ttggtcatat tgggggttaa aatcagaatt gttctcgttt cctgtttgcg
     3061 aaaagattgc gccagaatat tcggttcata tcccagcctt tcggcggctt cgtagactcg
     3121 tctgaccgta ttttcgctga tggtgctttt attattcagc acccgcgaga cagtcgaggc
     3181 ggacacgttc gcttccttcg ctacatcgaa tatggtaacc gtcattttat tcccttgttc
     3241 gttacttctt tcgcttattg tgcaatcgtt tgcataaatt attctatata ctccggcggc
     3301 atttgtcaat tgccaaaatg tcatcatttg aaacatgacg aaagtcataa aataagtaga
     3361 tcgaaataat tgaaaaacaa gacggaattt ttctgaaatc ggcggaatat aaaaaaagca
     3421 aaagcggatg cccgaagaaa aatatactaa aatatgaata gctttggagc atttaaaact
     3481 atgtcatttg ttcatcttca tcttcatacc gaatattccc ttttggacgg gttcacgaaa
     3541 atcaacaaac tgatgacccg tgtcaaagaa atgaatatgc ctgccgtcgc aatcacagac
     3601 cacggaacga tgttcggagt cgtggaattt ttcttcgcgg caaaggaggc gggtattaaa
     3661 ccgatcatcg ggctggaagg atatctttcc aaaggatcga tgacctctcg tgatcccaag
     3721 gaaaaaactt cttcccattt gctgttgttg gcaaaagaca tgaccggata caagaatttg
     3781 ttgcagatcg cgagtgcctc ccaattggag ggatattatt atcatccgcg catcgatcat
     3841 gaatatcttg cggaacatgc ggaagggtta atcgcgactt cgggatgtat ggcggcggaa
     3901 ctgccgcgga tgatcataaa cggagagagt gaagcggcta agtcgcaatt ggattggtat
     3961 cttcaggtat tcggacgaga aaatttttat atcgaattgc aggatcacga tgtcccggaa
     4021 ataaaaaaac tgaataatgt tttattacgt ctgggaaagg aatatgatat cccgtttgtg
     4081 gcgacaaatg acacccatta tgtcgatccc gaagattgga aatatcagga tattatgctc
     4141 gccatccaaa ccggtgcgaa actgacggat agcaaccggt tcagaatgtc ggataagtcc
     4201 tattatttgc gttctccgga agaaatggag aagctgttcg gatatatccc gggtgcgatt
     4261 gaaaacacat tggcgatcgc ggaacgctgt aatgtcgatt taagcagcaa agggcatcat
     4321 ctgccgattt ttccgttgga aacg
//
LOCUS       DAOMIV010000122         4339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20899_length_4339_cov_4.333567, whole genome shotgun sequence.
ACCESSION   DAOMIV010000122 DAOMIV010000000
VERSION     DAOMIV010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20899_length_4339_cov_4.333567"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="PKV59_03975"
     CDS             complement(<1..1623)
                     /locus_tag="PKV59_03975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQP45943.1"
                     /translation="MEKEEIQKEIPYWNYPQEKITEMLKSDPDSGLTEAEAENRLRET
                     GLNVLKERQKLTPLDLLLNQFKSPILLILLIATVISAALGNWVDAIIIFVIILFSAVL
                     SFVQEFNADKAAEMMQMRLKIKTDVIREGKKKSVPAEVIVPGDIIELSAGSIIPADAI
                     ILRAEDFFVNQSVLTGETFPVEKTAGTSAENAELEAMNNVVFMGTTVSSGYARCIVMK
                     TGSATAFGQVAKRMVLKPPATEFERGVNKLSYLLTIVMLVLVLIIFAANIYFRRPVLD
                     SLIFSIALAVGLTPQLLPAIININLSKGSQAMAKRGVIVRRLEAIENFGSIDILCTDK
                     TGTLTEGAVTLSSALDVQGKESEDVLRSAVLNAGLQAGMVNQLDEAILKKESLPESGI
                     EKLDEIPYDFIRKRLSVLVREGDEISLIMKGSVKDVLAVCSQARMDQQTVPLTSELIH
                     QIEEKMAEWNSEGNRVLGVAVKSVREHTHNLTKEDETDMVFIGFLLFFDQPKADAIQT
                     IVDLSELGINLKIITGDNVNAAKFIANAVGMKK"
     gene            complement(1664..2791)
                     /locus_tag="PKV59_03980"
     CDS             complement(1664..2791)
                     /locus_tag="PKV59_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQP45944.1"
                     /translation="MNPKIVVAGHVCVDLTPVIPHQAVEKIDQILIPGKLINTDGLSV
                     SAGGVVSNTGLALKKLGANAELMGKIGEDFLGDLLLDIFRRYGCEQGMIRSRDSATSY
                     TLVLAIPGLDRIFLHDPASNDSFCYDDLNFAHIQDATVFHFGYPPLMKRIYRNNGEEL
                     VRIFKKVKEYGVATSLDMAAIDPNSDAAKEDWQKLIREIIPYVDFFLPSMEELCSMIN
                     PPLFTEWTRRAKGKDLTEVISPKEVEPLADQLLSWGAKAVLIKCGAPGILLKTGSVSQ
                     IRQIGGNLITDPDEWANLSHFEMSYRPEKILSGTGAGDTCNAAFLLSVCRGYPWRRCL
                     QLAAGTGASCVEGYDSLSGLRSFEELEKKIDNGWQKVEWIH"
     gene            complement(2975..3601)
                     /locus_tag="PKV59_03985"
     CDS             complement(2975..3601)
                     /locus_tag="PKV59_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQP45945.1"
                     /translation="MKVLLINGSPHEKNCTYTALKEVADAIEKEGIETEIFHVGNKPI
                     SGCIVCDYCKRTRKNKCSIDTDSVNEALEKSKEADGFIFGSPVHFASASGAITSFMDR
                     IFRAGIYLEYKPAAAVVSCRRGGATSTFDQLNKYFTIRNMPVVSSQYWNMVHGNTPDE
                     VRQDLEGMQTMRTLGRNMAWMLKCIEAGKAAGISIPVAEKPVVTNFIR"
     gene            complement(3626..4210)
                     /locus_tag="PKV59_03990"
     CDS             complement(3626..4210)
                     /locus_tag="PKV59_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQP45946.1"
                     /translation="MENLPSRDQAWDLLTEYISSETLQRHVLTVEGVMRHFAGLYHED
                     EDEWGIIGLLHDLDYELYPDEHCIKVQEILREKGFPEEFIHAVASHGYGICCDVEPVT
                     NAEKVLYTIDELTGLIYAAALMRPSKSVMDLETKSVMKKFKSPAFAANVNRDLIREGA
                     EKLGMPLEDVITETISGMREIAPLIGLEGEITDK"
BASE COUNT         1187 a         1084 c          801 g         1267 t
ORIGIN      
        1 ttttttcatt cctacagcat tggcaataaa cttcgcggca ttaacattat ctccggtaat
       61 aattttcaaa ttaatcccca actcggagag atcgacgatg gtttgaatcg catcggcttt
      121 cggttgatca aaaaataaga gaaaaccgat aaaaaccatg tccgtttcat cttcttttgt
      181 caagttatga gtgtgttccc ggactgattt aaccgctaca cccaagactc gattcccttc
      241 ggaattccat tcggccatct tttcttcgat ttgatgaatt agctccgatg tcagcggaac
      301 cgtctgttga tccatacgag cttggctgca gacagccaaa acatctttaa ccgatccttt
      361 catgatcaaa gaaatttcat cgccttcgcg aaccaaaacg ctcaaacgct tgcggataaa
      421 atcatatgga atctcatcta atttttcaat tccggattcc ggcagacttt cttttttcaa
      481 aattgcttca tccaattggt tcaccattcc ggcttgtaat ccggcattca atacggccga
      541 tcgaaggaca tcttccgatt cttttccctg tacgtccaga gcgctgctta acgtaaccgc
      601 accttcggtc agtgtccccg ttttatccgt gcaaagaata tcgatgctgc cgaagttttc
      661 aatagcttcc agccggcgta cgatgacacc cctttttgcc attgcttgag aacctttgga
      721 gagattaata ttgataatcg caggtaagag ctgcggtgtt aatccgacgg ctaaggcgat
      781 cgaaaaaatt aacgaatcga gaaccggacg gcgaaaataa atatttgcgg caaaaatgat
      841 caacactaaa accaacatca caatagtcaa caggtaactg agcttattga caccgcgctc
      901 gaactcggtt gcgggtggct tcaaaaccat tcgtttggcg acctgcccga aagccgttgc
      961 cgaccccgtc ttcataacga tgcatctggc atatccggaa ctgaccgtcg tacccataaa
     1021 aacgacatta ttcatcgctt ccaactcggc attttcagcc gatgttcccg ctgttttttc
     1081 aacaggaaaa gtttcgccgg ttaatacgga ttggttcaca aagaaatctt ccgctcggag
     1141 gataatcgca tctgcgggaa taatactgcc ggctgaaagc tcgatgatat ctccgggcac
     1201 aatcacttct gccgggacag actttttctt accctcccga atgacatcgg tcttgatttt
     1261 taatcgcatt tgcatcattt cagccgcttt atcggcattg aattcctgga caaaagagag
     1321 aactgcgctg aacaagataa tcacaaagat aatgattgcg tccacccaat tccctaatgc
     1381 cgcggaaata acggtcgcga ttaataaaat caacaaaata ggacttttaa actgattaag
     1441 aaggagatcc aacggggtta atttttgcct ctctttgaga acattcaatc ccgtttctct
     1501 taatcggttt tcggcttcag cctccgtcaa ccccgaatca ggatcacttt ttaacatttc
     1561 ggttattttt tcctgcgggt aattccaata aggaatttct ttttgaattt cttctttttc
     1621 cattttcgat acctcccttt tgtgttctcg gattaaactc tgtttaatgg atccattcga
     1681 ccttctgcca accgttatca atcttttttt ccagttcctc aaaagatctc agaccgctga
     1741 gtgaatcata tccttcgaca caagaagcac ccgtcccggc ggccaactgc aaacagcgtc
     1801 gccagggata tccgcggcaa accgatagca aaaaagccgc attacaggta tctcccgctc
     1861 ccgtgcccga gaggattttt tccggtctgt aactcatttc gaaatgggac aaattcgccc
     1921 attcgtccgg atcggtgatc aggtttcctc cgatttgtct gatttgagaa acggaaccgg
     1981 tttttaataa aattccgggt gcaccgcact tgattaagac cgcttttgct ccccatgaca
     2041 gcaattgatc cgccaacggt tcaacctctt tcggcgatat cacctcagtc aaatctttac
     2101 ccttcgcccg tctcgtccat tccgtaaaaa gcggtggatt gatcatgctg cacaattctt
     2161 ccatacttgg caaaaagaaa tccacatatg ggataatctc ccgaatcaac ttttgccaat
     2221 cctcttttgc ggcatcggaa ttcggatcga tagctgccat atccaatgac gtggcgacac
     2281 cgtattcttt aactttctta aatatccgaa ccagttcttc accgttattc cggtaaatac
     2341 gtttcatcaa tggagggtat ccgaaatgaa aaacagttgc atcttgaata tgcgcaaaat
     2401 tcaaatcatc ataacaaaaa gaatcattgg aagcgggatc atgaagaaaa attctgtcca
     2461 atccgggaat tgccaaaaca agcgtgtaag aggttgccga atccctcgaa cgaatcatgc
     2521 cttgttcgca tccgtaccgc cgaaaaatat ccagcagcag gtctcccaaa aaatcctctc
     2581 cgattttccc catcagctcc gcattcgcgc ctaatttttt caaagctaaa ccggtattgg
     2641 agaccacacc gcctgccgat acagataatc cgtcggtatt gattaatttt cccggtatga
     2701 gaatttgatc gattttttcg accgcttgat gggggataac cggtgtgaga tctacacaaa
     2761 catggccggc tacaacgatt tttggattca tttatactcc gattggttat tccgaatagg
     2821 taaataatct acaaaacaat tatatctgct gaacgacaag ccaagcaaca attcaagttg
     2881 agcgggttta cgaaaaatga aacccatcga atgaaattaa aataagagaa ccgctatcgt
     2941 tttggatggc ggctctcttc gtttccataa gacatcagcg gataaaattt gtcacgacgg
     3001 gtttttccgc tacaggaatc gagataccgg cggcttttcc ggcttcgata catttcaaca
     3061 tccaagccat atttctaccg agagtacgca ttgtctgcat tccttccaga tcttgccgta
     3121 cttcatccgg cgtatttcca tggaccatgt tccaatattg cgatgagacg accggcatgt
     3181 ttcgaatcgt aaaatattta ttcagttgat caaacgttga ggttgcgcct cctctgcggc
     3241 agctcacaac cgccgcagct ggtttatatt ccaaataaat gccggcacga aatattcgat
     3301 ccataaaact ggtaatggct ccagatgccg aagcaaagtg gacgggagag ccgaagataa
     3361 atccgtcggc ttccttcgat ttctccaaag cttcattgac tgaatcggta tcgatggaac
     3421 acttgttttt tcttgttcgc ttacaataat cgcagacaat gcaaccgctg atcggtttat
     3481 tcccgacatg aaatatctcg gtttcaattc cttccttttc aatcgcatca gcgacttcct
     3541 tcaaagccgt ataagtgcaa ttcttttcgt gaggacttcc attaatcagc aatactttca
     3601 tcttggttta ctctcctttt ctcctttatt tatcggtaat ttccccttcc aaaccgatca
     3661 atggggcaat ttctcgcatt cccgaaatcg tctcggtaat cacatcttcc aatggcattc
     3721 ccaatttttc cgccccttca cgaatgagat cccgattgac attggccgca aatgcgggag
     3781 atttaaactt tttcataacc gattttgtct caaggtccat cacactttta gatgggcgca
     3841 ttaaagcggc cgcataaatc aaaccggtca gttcatcaat tgtatataac actttttcgg
     3901 cattcgtgac cggctcgaca tcgcaacaaa tcccataacc atgacttgca accgcatgaa
     3961 taaactcttc cgggaaacct ttttctctca ggatctcctg aacttttata caatgttcat
     4021 cgggatacag ctcataatcc aaatcatgca acaatccgat aattccccat tcatcttcgt
     4081 cttcatggta aagtccggca aaatgacgca tcacaccttc aaccgttaaa acatgcctct
     4141 gtaatgtttc gctcgaaata tattccgtca ataagtccca agcctggtcc ctggaaggaa
     4201 gattctccat aatctttatt ctgtcctctg ctttctgtaa atctttttct attatacatt
     4261 cagattaatg tgaattttta tgtcctgtaa ttagagttgt aaaaagaaat attataaatc
     4321 taattttcct agtcatcta
//
LOCUS       DAOMIV010000123         4327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20981_length_4327_cov_4.141152, whole genome shotgun sequence.
ACCESSION   DAOMIV010000123 DAOMIV010000000
VERSION     DAOMIV010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20981_length_4327_cov_4.141152"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2524
                     /locus_tag="PKV59_03995"
     CDS             <1..2524
                     /locus_tag="PKV59_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQP45947.1"
                     /translation="GKSGVETARFIGELPEVERICVEFCYTFMPVQDFVSYPFQLIDI
                     HVKHAVMLLNTVPWLKNIPEDIFLNFVLFYRVNNEYIEDYKNRFFEELWPRVRNKSDR
                     DAILEVNYWCYEKATYKESDDRTASPLTVIRRTYGRCGEESTLLVSALRSVGLPARQC
                     YTPRWAHCDDNHAWVEAYSDGKWHYLGACEPEPVLDKGWFTAAASKAMLIHSKVFNSD
                     MKGELIAGRTPVFCMINNLKTYAKTTCLNVFVRKDNRPFAGAVVRCEIVNYSELYPLL
                     TAETDENGFASFVLGRGDIILHVHDGNHFLMKKVDLRRQSEITVDFDEAVENKPGNFD
                     FELIPPAEDLSNPVEISRETAEMHEKRLEHCNRVRLQTEMSFAENPVLKNLKLDSDWD
                     KSLVKQYLTDAKGNFDEIIDFLQCDSFTTADKAAILNTLREKDFVDITSETLKEYLEA
                     SLPYKGLFPEDVYIHSVLAPRIENEMILPQRKQLKAYFDRLSEPIHSASQLWDYLRRA
                     VILQDEYGYDSLVCDAKNILSTGICDSHSLDILFVSTARSLGIPARINSSTSQKEFYD
                     GEKYCGIGEDSKPVDRNNSRLIIRNQSGKDLNYFSQISIGKFSAGVYQSLVYWNQTIG
                     ETWELPVEAGEYRVITTVRQIDGSVKCKVYFISVPENQSVVLPVDLAKEDILGKLKYA
                     KIENVPLMSESGENTDLYSELREDYTILAFADPGKEPTEHLFNEWIRLAEKYRSPDLG
                     VMIVLDDRRGLQNPTLQKVLQAIPGTRVLIMDNPRYLKEFHLLMGVGDERKPFVLVIN
                     NHREGLYAFSNYNVGTGQTLLNIISEDSSGRKSEKKPSGNIK"
     gene            2581..4314
                     /locus_tag="PKV59_04000"
     CDS             2581..4314
                     /locus_tag="PKV59_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP45948.1"
                     /translation="MKKLSLFILIISIVLIGLTGCSGSKESTTTAPATVSTESVSEPT
                     VEATAETEKETVATEETVATEPVTSGKPETASGTITVYDTNEIRTLVQWAASDTQSFT
                     VLNNVFEGLYRLGIDHQPEPALATGYTVSDDKLTYTFTLRDGIQWSNGTPITAKDFVF
                     AWLKQMGPDATNGYSFIMNDYIVNGTEYNEGKVKAEEVGVKAIDDKTLEVNLKTPTPY
                     FIRLTTLSMYFPLNEEFVTSQGDQYGVKAENMIYSGPYVITSYDPVVGTTLEKNEKYW
                     DAANVAVKNVNIKIIKDQSAALNAYKAGELSKVLLSSTDVAVYKNDPEFHSQSEFRTT
                     YLQFNTTAKGLDNVNIRKALGYSIDRSILADTILADGSAAAEGLVSFGVAGDGTSTFR
                     ELNGNLSPFDPEKAKEYWAKGVEELGEAPQLTLLIADDSVTKTVATFVQSQFKDNLGI
                     DVAIDSKTSKARNELMDNNNYQFAITAWGADYDDAMTYLDLWTNGTPYRGNYSNETYN
                     KLIADAKKETDEAKRLTMMLDAEKILLDTDAVISPLYYRGFAYLQKSNIENLVTHPFG
                     NPIEFKYARIK"
BASE COUNT         1267 a          879 c         1023 g         1158 t
ORIGIN      
        1 tggtaagtcc ggagttgaaa cagctcggtt catcggagaa ttgccggaag ttgaaagaat
       61 ttgcgtggag ttttgttata cattcatgcc ggttcaagac tttgtgtctt atccttttca
      121 attaattgat attcatgtga aacatgcggt catgttgttg aataccgttc catggctgaa
      181 gaatattccg gaagatattt ttctgaattt cgttctcttc tatcgcgtaa ataacgaata
      241 tatcgaagat tataaaaatc ggttctttga agaactgtgg ccccgtgtcc ggaataaatc
      301 cgatcgggat gccatcttag aagtgaatta ttggtgttat gaaaaagcta cttataaaga
      361 atcggatgac cggacagcct caccattaac ggttattcga aggacatacg gtcgctgcgg
      421 agaagaaagc acattgctgg tcagcgcttt acgcagtgtc gggctgccgg caaggcaatg
      481 ttacactcct cgttgggctc actgtgatga taatcacgcc tgggttgaag catattcgga
      541 cgggaaatgg cattatctcg gtgcgtgtga acccgaaccc gttttggaca aaggttggtt
      601 taccgcggca gcttcgaaag cgatgttgat tcattccaaa gtctttaatt cggatatgaa
      661 aggtgagttg atcgccggtc gcacccctgt tttttgtatg ataaacaacc tgaaaacgta
      721 tgcaaaaaca acctgtttga atgtttttgt gagaaaagac aaccgtccat ttgccggagc
      781 cgtggtccgg tgtgaaattg tgaattacag tgaactatac ccgttattaa ccgccgaaac
      841 cgatgaaaac ggcttcgctt catttgtgct cggcagaggg gatatcattt tacacgttca
      901 tgacggaaat catttcctga tgaaaaaggt tgatctcaga cggcagagcg aaatcaccgt
      961 tgattttgat gaagccgttg aaaacaaacc ggggaatttt gattttgagt tgattccacc
     1021 cgctgaggat ttatcgaatc cggttgaaat ttcgcgggaa acagcagaaa tgcatgagaa
     1081 acgtttagag cattgcaacc gggttcggtt gcaaaccgaa atgagctttg cggaaaatcc
     1141 ggtgctgaaa aatctcaaac tcgattcgga ttgggacaaa tctttggtga agcaatacct
     1201 tacggatgcc aaaggaaatt tcgacgagat aattgacttt ttacagtgcg actccttcac
     1261 gacagcggat aaagcggcaa ttctgaatac tttgcgcgaa aaggactttg tcgatatcac
     1321 ttcagaaaca ctgaaagaat atttggaagc ttctcttcct tataagggct tatttccgga
     1381 agatgtctat attcattccg ttttggcacc tcggattgaa aatgaaatga ttttgccgca
     1441 gcggaaacaa ctgaaggcgt attttgaccg attgtcggaa ccgattcatt cagcttccca
     1501 gctatgggat tatttacgaa gggctgttat tcttcaggat gaatacggtt atgattcttt
     1561 ggtttgtgac gcaaaaaata tcctttcaac cggaatttgc gattctcatt cattggatat
     1621 cttgtttgtc agtactgcaa ggagtttggg aatccctgcg cgaataaaca gttccaccag
     1681 ccagaaagaa ttttatgacg gcgaaaaata ttgcggaatt ggtgaagatt caaaaccggt
     1741 cgatcgaaac aattcccgac tgattatccg aaaccaaagc gggaaggatt tgaattattt
     1801 tagccaaatc tcgatcggaa aattttccgc gggggtgtat caaagcctgg tttattggaa
     1861 tcaaacaatc ggagagacat gggagttacc ggtcgaagcc ggtgagtatc gtgtaattac
     1921 aactgtacgc caaatcgacg gcagcgtgaa atgcaaggtg tattttatat cggtaccgga
     1981 aaatcagagt gttgttctac cggttgattt ggcgaaagag gacattctcg gcaagctgaa
     2041 atatgccaaa atcgaaaatg tcccattaat gagcgaatcg ggtgagaata ccgatctgta
     2101 ttccgaattg cgagaggatt ataccattct tgcctttgcc gaccccggca aagaacccac
     2161 cgaacatctg tttaatgagt ggatccgttt ggcggaaaag tatcgttcgc ccgatttagg
     2221 ggtcatgatt gttttggatg atcgccgggg actccaaaat ccgacactgc aaaaagtgtt
     2281 gcaagccatc cccggaacca gagttttgat tatggataat ccccgttatc tgaaagagtt
     2341 ccatctgttg atgggagtgg gagatgaaag aaaaccgttc gttttggtga tcaataatca
     2401 tagggaggga ttatatgcct tcagtaacta taacgtcgga acaggacaaa ccctgctgaa
     2461 tatcatcagc gaagattcgt cggggcggaa atcagagaag aaaccatcgg gaaatataaa
     2521 atgatttttc aaacggaaaa taaaatgcca tccgcatttt aaatatatta aggagaaaaa
     2581 atgaagaaac tatcgttatt tattttgatc atcagcatcg ttctgatcgg attgacgggc
     2641 tgctccggtt caaaagaaag tacaacaacc gcaccggcaa ccgtttcaac cgaatcggtt
     2701 tcggaaccga ccgtagaggc gacagccgaa actgaaaaag aaaccgttgc gacggaagaa
     2761 actgtggcga cggaaccggt aacttccggt aaaccggaaa cggcgtccgg aacaattacc
     2821 gtttatgaca ccaatgaaat tcgaacactg gtccaatggg ctgcttccga tactcaatcc
     2881 tttaccgttt taaacaatgt ctttgaaggt ttgtatcgcc tcggaatcga tcatcaaccc
     2941 gaaccggctt tggcgaccgg ttataccgtt tccgatgaca agttgactta cacttttacc
     3001 ttacgcgacg ggattcaatg gtcaaatggg actcccatta ccgccaaaga ttttgttttt
     3061 gcttggttaa agcaaatggg tccggatgcc acaaacggtt attcgtttat catgaatgac
     3121 tatattgtca acggaaccga atacaacgag ggcaaagtca aagcggaaga ggttggcgtc
     3181 aaggcaatcg atgataagac gttggaagtt aacttgaaaa ccccgactcc ctattttatt
     3241 cggttgacga cattgagcat gtatttcccg ttgaatgagg aattcgtcac ttcccaggga
     3301 gatcaatacg gtgtaaaagc cgagaatatg atttattccg gtccctatgt gatcaccagc
     3361 tatgatcccg tcgtcggtac gactctggaa aagaatgaaa aatattggga tgcggcgaat
     3421 gtggcggtca agaatgtgaa tatcaaaatt atcaaagacc aatcggccgc attaaatgca
     3481 tataaagccg gagaattatc caaagtctta ttgtcctcga cagatgtggc ggtttataaa
     3541 aatgatccgg aatttcattc ccaaagcgag ttccggacta cctacctgca attcaacacg
     3601 actgccaagg gactggacaa tgtcaacatt cgtaaggctt taggttattc gatcgatcgt
     3661 tcgatccttg ccgataccat tcttgccgac ggttccgctg ctgcagaagg actcgtttcc
     3721 ttcggtgttg ccggtgacgg aacatcgact ttccgcgaat taaacggaaa cctctctccg
     3781 ttcgatcccg aaaaagcgaa agaatattgg gcgaagggtg ttgaagaatt aggcgaagcg
     3841 ccgcagctca ctttactcat tgccgatgat tcggtgacaa agacggttgc cacgttcgtt
     3901 caaagccagt ttaaggataa tttggggatt gatgtcgcaa tcgattcaaa aaccagtaag
     3961 gcccgcaatg aactgatgga taacaataac tatcaatttg ctattaccgc ttggggcgcc
     4021 gattatgatg acgcaatgac ctatctggac ttatggacaa acggaacgcc ttatcgcggc
     4081 aattactcta acgaaacata taacaaacta attgcggatg cgaaaaaaga aacagatgaa
     4141 gcgaagcggc tgaccatgat gctcgatgcc gaaaagattt tattggatac cgatgccgta
     4201 atttcgcctc tttattatcg cggatttgct tacctgcaga agagtaatat cgagaatttg
     4261 gtaacccacc cctttggaaa tccgattgag tttaaatacg ccagaattaa atagtctttt
     4321 cgttttt
//
LOCUS       DAOMIV010000124         4327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_20982_length_4327_cov_4.128979, whole genome shotgun sequence.
ACCESSION   DAOMIV010000124 DAOMIV010000000
VERSION     DAOMIV010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20982_length_4327_cov_4.128979"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PKV59_04005"
     CDS             <1..657
                     /locus_tag="PKV59_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQP45949.1"
                     /translation="AAKSGRRSSEISLMAVTKKKSAEVIRCLIDLNIRYFGENYPDET
                     LGKIEAFKETEQAIRLCMIGHLQSRKINIVAEHFDEYHSLDRLKTAVRLNQILKIINK
                     TMPVFLEINIGNEDSKSGWSSENGNLSDQLLKDLEVIQTLSQLKVDGLMALPPISENP
                     EESRPYFVRLRYMLEKLNDNYGYSLHHLSMGTSIDYPIAIEEGATFVRIGTALTGPRI
                     "
     gene            680..931
                     /locus_tag="PKV59_04010"
     CDS             680..931
                     /locus_tag="PKV59_04010"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQP45950.1"
                     /translation="MSNFFVILVSTFVNIMSILLLIYVVISWFVPPYNEFRMKLDQLM
                     NQFLDPIRRIMPTIGMFDFSPIILMLLLQFFESIVKIIF"
     gene            complement(1002..1931)
                     /gene="argF"
                     /locus_tag="PKV59_04015"
     CDS             complement(1002..1931)
                     /gene="argF"
                     /locus_tag="PKV59_04015"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQP45951.1"
                     /translation="MKKKDFLSIADFSESELIHILDVAQALKKELKEGGNKPILKGKS
                     MALVFQKPSLRTRVSFEMGMHQLGGYAFYLSPDEVGLGKREAVKDVAMVLAGYVDAIM
                     ARVFQHQHLIELAEFSGIPVINGLSDYNHPCQAMADALTIIEEFGKLKGINIAFIGDG
                     NNVAVSLMDIATKLGANFSIANPKGYDMPEEAISAAKEFAKLSGSRLVFSNDPYEIVK
                     NAQVIYTDTWISMGQESEAEERKKAFIPYQVNQKLVDAAPKDVIVMHCLPSHRGQEIT
                     DEVQDGPHSRVIPQAHNRLHAQKGIMAYLLADL"
     gene            complement(1928..2821)
                     /locus_tag="PKV59_04020"
     CDS             complement(1928..2821)
                     /locus_tag="PKV59_04020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQP45952.1"
                     /translation="MFILWSLIDMMAKSLQSKLVLISGGSSGIGLATAKRMAREGASF
                     LILARDEEKLTNARELILREAVSNDQFVETLSVDMRNYEELRNKLVPILDRLGTPDYA
                     MHSAGVVYPALFEDITIEQFHWMMDTNYFAAVNLFQIIIPRMKARRSGHVIAMGSASS
                     FIAIWGYSAYSGSKYAIRGLMETLRCELKPFNIQTSIVFPPDTDTPQLAYENQFKPAI
                     TKEVSGTIKPLSADFVADKIYRGVMKNQYQIIPDRDTQLTYKAAHILGTGIFKVLDMV
                     TDKAIKKYGLESKNIYTEENK"
     gene            complement(3123..>4327)
                     /locus_tag="PKV59_04025"
     CDS             complement(3123..>4327)
                     /locus_tag="PKV59_04025"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HQP45953.1"
                     /translation="LEMTQALSNQGYDVSAAENLIPEGIQLFEIGDIDNLRVLTAKLL
                     NALNNAPKIADHPYHQHEYPMSWEEIYRAMPISSQASSSRPEWKNTFKNKIYQGWIGQ
                     LAGGSFGTAIEGYIGSQIELIYGEVDSYITTPETTNDDVVYELAFLDAFAKNGRYVTS
                     QQVGLEWIKQIQFGWSAEWIAIRNLNMGIFPPESGRFLNFYSDWIGAQMRGMVCGMLA
                     PAWPVEAARLAFTDAVVSHSNNGVYGEIAAAVMTALSFEMENPRDIIQETLKYLPAKS
                     EYVEKYLIVLDILRTEEDPNIARGRIEPHFIRYNWIHSYPNMAADLYALWYGNGDFSE
                     SMKLLAKSGNDVDCNAGLVGNILGIIRDVPRKWSDPIGDLLETYIKGKERLSIRHLAE
                     KTSELAQQ"
BASE COUNT         1233 a          892 c          830 g         1372 t
ORIGIN      
        1 gccgctaaaa gcggtagaag atcctcggaa atttctctga tggctgtgac aaagaaaaaa
       61 tcggcggagg tgattcgatg cctgattgat ttaaatattc gatatttcgg cgaaaattat
      121 ccggatgaaa ctctcgggaa gatagaagca tttaaagaaa cggaacaggc aatccgtctt
      181 tgtatgatcg gtcatttaca gagtcgaaaa attaatattg tcgcggaaca ttttgatgaa
      241 taccattctc tggatcgttt gaaaaccgct gttcgattaa atcaaatttt aaaaataatt
      301 aataaaacga tgccggtttt tctggaaata aacataggga acgaagacag caaatccggt
      361 tggagctctg aaaacggaaa cctgtccgat caattattaa aagacttaga agtcatccaa
      421 actctctctc aactaaaggt tgacggattg atggcacttc ctccaatttc ggaaaatccc
      481 gaagagagtc gtccctattt tgtacgattg cggtatatgc ttgaaaaatt gaatgataat
      541 tatggctatt cgttgcatca tctttcaatg ggaaccagca ttgattatcc gattgcgata
      601 gaagaagggg caacatttgt ccggatcggg actgcattga caggccccag gatataatat
      661 ttcaaataag aggaaataaa tgagtaattt ttttgtaatt ttagtttcga cttttgtaaa
      721 tatcatgtcg atattgttat taatctatgt ggtaatttcc tggtttgtac ccccttataa
      781 tgaatttcga atgaaattag atcaattgat gaatcaattc ttggatccca ttcggcgaat
      841 tatgcctact atcggaatgt ttgatttcag ccccatcata ttaatgcttt tattgcaatt
      901 ttttgagtct atcgtaaaaa ttatcttctg aataaatgga agcaaaaaag agctgcactt
      961 atttactgaa gagtgcagct cttttttcga tgaaaataat gttataaatc agcaagtaaa
     1021 taagccataa tccctttttg ggcatgcaaa cgattgtgag cttgcggaat aacacgggaa
     1081 tgaggaccat cttgaacttc atcggtaatt tcctgtccac ggtgagaagg taaacaatgc
     1141 atcactatga catctttagg cgcggcatct actaattttt gattgacttg ataaggaata
     1201 aaggctttct ttctttcttc tgcctccgat tcctgcccca tactgatcca agtgtcggta
     1261 taaataacct gtgcattttt cacaatttca tatggatcgt tggaaaaaac aagcctgctt
     1321 ccgcttagtt ttgcgaattc ctttgcggct gaaattgcct cttcaggcat atcatatccc
     1381 ttgggattag cgattgaaaa gtttgctcct aattttgtcg ctatatccat taatgaaacg
     1441 gccacgttat ttccgtcgcc gataaatgcg atattgatcc ctttcaattt gccgaattct
     1501 tcaattatgg ttaacgcatc ggccattgcc tggcaaggat ggttataatc ggacaaccca
     1561 ttaataaccg gaataccgga aaattcggct aattcaatta aatgttggtg ttggaatacc
     1621 cgtgccataa ttgcgtcgac atatcccgct aaaaccatcg ccacatcttt taccgcctct
     1681 cgtttcccga ggccgacctc atccggtgaa agataaaatg catagccgcc caattgatgc
     1741 attcccattt cgaaactgac tcgggttctt aaagatggct tttgaaaaac caaagccata
     1801 ctttttcctt ttaaaatcgg cttgtttcct ccctctttta attccttttt caatgcttgt
     1861 gcgacgtcta atatatgaat taattcggat tctgagaaat cagcgatcga taaaaagtct
     1921 ttttttttca tttattctcc tcggtataaa tgttttttga ttccaaccca tattttttaa
     1981 ttgctttgtc tgtaaccatg tccaatactt taaaaattcc agttccgaga atatgtgcgg
     2041 ctttataagt taattgagta tctcggtccg ggataatttg gtattggttt ttcataactc
     2101 ctcgataaat tttatcggct acgaaatccg ctgaaagcgg tttaattgtc ccgctaacct
     2161 ctttggttat ggccggctta aactgatttt cgtatgcaag ttgcggggta tccgtatccg
     2221 gtggaaaaac gattgaagtt tggatattaa acggctttaa ttcacaacga agtgtttcca
     2281 tcaatccgcg aattgcgtat tttgatccgc tataagccga atatccccag atggcaataa
     2341 aagaagaagc ggagcccatc gcaataacat gtcccgaccg tctggctttc atcctcggaa
     2401 tgataatttg aaaaaggttg actgcggcaa aataatttgt atccatcatc caatgaaact
     2461 gctcgatcgt tatatcctca aaaagagcgg gataaacaac tccggcggaa tgcatcgcat
     2521 aatccggcgt tcctaaacga tccagtatgg gaaccaattt atttcttaat tcctcataat
     2581 ttctcatgtc gacggataat gtttcaacga actgatcatt cgaaaccgct tctcgaagga
     2641 tcaattctct ggcatttgtc aacttttctt catctcgtgc cagaattaaa aaagaggcac
     2701 cttctcttgc catcctttta gcggttgcga gaccgatacc gcttgaaccg ccggaaatca
     2761 agaccaattt tgattgaagt gattttgcca tcatatcaat caatgaccac aggatgaaca
     2821 atttccgccg gagcaggatg aacaacctga cgaggaggaa ttttccgatg aaccttggct
     2881 gtaaaatttt gaaattaatc gtttcgtgtt attgctgccg catttctgac attttgcggg
     2941 atcattcgct ttagcaatat cccggattag ctcaaatttt gtattacaat cttgacagta
     3001 atattcataa atcggcataa tttgatctcc tttctgagta tctgaaaata aatccgcttt
     3061 ttgttcaatc tttaaataaa tattctcgcg aaattattat aagactaaat cttttggtcg
     3121 atttattgtt gtgccaattc ggatgttttt tcggcgaggt gtcggatcga taatcgttct
     3181 ttccctttga tatatgtttc gagtaaatcg ccgatcgggt cagaccattt tcggggaaca
     3241 tcccggataa ttcccaagat atttccgacc agcccggcat tgcaatccac gtcatttcct
     3301 gatttggcaa gcagtttcat ggattcggaa aaatctccgt tcccgtacca cagagcgtat
     3361 aaatcagccg ccatattcgg ataagaatga atccagttat agcggataaa atggggttca
     3421 attcttccgc gtgcaatatt cgggtcttct tccgttctaa gaatatccaa aacgatcaga
     3481 tatttttcaa catactcgct ttttgccgga aggtatttaa gagtctcttg aatgatgtct
     3541 cgcggatttt ccatttcaaa tgacaaagct gtcataaccg ctgcagcaat ttctccatag
     3601 acaccgttgt ttgaatggga aacgaccgca tcggtgaatg ccaatcgtgc cgcttcgacc
     3661 ggccaggcag gggctaacat cccgcaaacc attcctcgca tttgagcgcc gatccaatcc
     3721 gaataaaagt ttaaaaatcg gccggattcg ggcggaaaaa ttcccatatt cagatttcga
     3781 atcgcaatcc attccgctga ccaaccgaat tgaatttgtt taatccattc aagtccgact
     3841 tgttgtgaag ttacatatct gccgttcttt gcaaaagcat ccaagaaagc aagttcatag
     3901 accacatcat catttgtcgt ttccggagtt gttatatagg aatcaacttc tccgtaaatt
     3961 aattcgattt gtgatccgat atacccttcg atcgctgtcc cgaatgagcc tccggctaac
     4021 tgacctatcc atccttgata tattttattt ttaaatgtat ttttccattc ggggcgagat
     4081 gaagaagctt ggctgctgat aggcatcgcc cgatagattt cttcccaaga cattggatat
     4141 tcatgttgat gataagggtg gtcagcgatt ttcggtgcat tatttaaggc atttaataat
     4201 tttgcggtta aaactcgtaa attatcgata tcaccgattt cgaataattg aatcccttct
     4261 gggattaaat tttctgccgc agatacatca tacccttgat tacttaatgc ttgggtcatt
     4321 tcaagat
//
LOCUS       DAOMIV010000125         4309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_21126_length_4309_cov_3.362012, whole genome shotgun sequence.
ACCESSION   DAOMIV010000125 DAOMIV010000000
VERSION     DAOMIV010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21126_length_4309_cov_3.362012"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            606..1418
                     /locus_tag="PKV59_04030"
     CDS             606..1418
                     /locus_tag="PKV59_04030"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HQP45954.1"
                     /translation="MTPIVYDKKDPKRLMDEEIQTVLEKFPLISLNEMENVQLLDRID
                     RKYVLPIQKITDVLNDLTEDYRALFVRGRRLNHYRTLYFDTEDFLLYNLHINDRKNRY
                     KVRSREYVDSQSSFLEVKEKTNKGRTVKSRIPIDFPISQMTSEMENWVNSQIPMEDLH
                     FEPKLWTNFTRITLVGINAPERVTVDFNLTVFNDFRAEQMNDIVIAEVKTEKTDQHSP
                     FVEKMKMMHIHPQEFSKYTIGVSEIYDGMKKNKIKPTLLKISKIKREAQFYE"
     gene            1411..2043
                     /locus_tag="PKV59_04035"
     CDS             1411..2043
                     /locus_tag="PKV59_04035"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HQP45955.1"
                     /translation="MNNPIMILIGFVYNLLVAMILIRFIYYPKSSVQSHVFTFLAFNT
                     VIFFVINFLSSTEMGIGMGFGLFAVFSLLRYRTDPLPIREMTYLFIIVALPIMNATGI
                     ADNNWAGMVIADGLILLTIWILEKGWGFHYEISKTIIYEKIDLIVPEKYDELKADLEK
                     RTGVKINKIAIGKIDFMRDIAYLEVFSDQDQKYNWNRRMEAATASDIDDD"
     gene            2064..2750
                     /locus_tag="PKV59_04040"
     CDS             2064..2750
                     /locus_tag="PKV59_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQP45956.1"
                     /translation="MARKVLVIDDTRNIQIMLRDFLESQGFEVEIAGDGIEAIDSIER
                     SQPDLILLDIMMPNMDGYQFISQLRKTSDLPVIMITAKQQEADIVHGFELGADDYITK
                     PFKLRELLMRMRAVLRRSTPREEEKPITIIGDLQLDRSRHKVTLGDEPVELTPLEFFV
                     LDILMDSQGKVVRKEDLCIRLIENGFSGSESTLKIHIRNIRMKLRDELDQPKYIETVF
                     GIGYRFLEGS"
     gene            2750..4192
                     /locus_tag="PKV59_04045"
     CDS             2750..4192
                     /locus_tag="PKV59_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP45957.1"
                     /translation="MKKNIRSKMIFSYLLIALITVIVVSLLVRWNSGQSLMNMVVEQQ
                     TANMKEAVQTYYEENGTLDGFFDRYVRNGFASGLPAPEAVNSDSDAVAQMKPVIKPQD
                     NPQNSPDIRGLHGLVDAQYRALIPTMGYEPGEIVPENMMKDAVPVEYDGEIIAWILPD
                     TQLKFKLSNEEQLYLERTTRAIIFAGIIGVCLAILMGFLFSKRILGPVQRLTKASKAL
                     AAGNLDQTVPVTSEDEIGQLTQTFNQMSTDLVRADLQRKRLTADITHDLSTPIQIISG
                     YIEMAEDEGIEINSNRLQIIKSELQRLKRLVDDLTTLSQVEAGGLDFQFERIRPAEML
                     ERVYQTYLPILKKQNIRFVYDVSEDCPDILADEDRMIQVLHNLIENALRYTPENGTIS
                     LKAYFDEFVRIEVRDSGAGIDSEDLPYVFDRFYRADKSRNVNSGKMGLGLAICKALII
                     AQKGKISAESEGIGKGTTFLILFPPYNGME"
BASE COUNT         1328 a          831 c          984 g         1166 t
ORIGIN      
        1 cggtttgccg gatttgctct tgtagggacc ggcttgatga tttacgctgc cgaattgctg
       61 tcagccagaa aatcacatta tgaaaagaac gattcgaatg aaacccaaca ataaaaaagg
      121 aaagcggttc cggtgataaa tctgacgccg gccttggtcg gatacggagg cgtatcagac
      181 aatacccaac tccgtcggtc cacgcctgcc ccctttcgga aaaagcgggc aaataagcgc
      241 cgtataacga acaatgaaac cggggatacg tcgaagaaga gtgtggtttc tcccgggaac
      301 gcgtgtatgt cacacaagat tgagtgggcg gcttaaccgc accgttgaag gagagcgaac
      361 ctacggagat gagcgatcct gtcaccgttt tctgaaaaat tcacgcgatt actgccacgt
      421 cagattttcg gggttgttcc tttcttaaat gccccgattc aaggctggtg tacttctttt
      481 tattcctata caaaaagtaa accatttccg ctttccgaaa cattattatc tatggtaatt
      541 aggatgaagg gagaaaaaca gatatgaact catttcttag agaaaacctt aaattaacgc
      601 aaacgatgac accaatcgtt tatgacaaga aagatcctaa acgtttgatg gacgaagaaa
      661 tacagacggt gctggaaaaa ttccctctga ttagtttaaa tgagatggaa aatgtccagt
      721 tgttagacag aatcgaccgt aaatatgtac tacctattca aaaaatcacc gatgttttga
      781 atgatcttac agaggattat cgagcgttgt ttgtgcgggg cagaaggttg aatcattatc
      841 ggacgctata ttttgatacc gaagatttcc tgttgtacaa cttacatatc aacgatcgta
      901 aaaatagata taaggtacgc agtcgagaat atgtcgatag tcaatcctct tttcttgagg
      961 taaaagaaaa aacaaataaa ggaagaaccg tcaagagccg aattccgatt gattttccga
     1021 tcagtcaaat gacaagcgag atggaaaatt gggttaacag tcaaattccc atggaagatc
     1081 tgcactttga accaaagctt tggacaaatt tcacacggat tactttggtg ggaatcaatg
     1141 ctcccgaacg tgtgacagtg gattttaatc taacggtttt caatgatttt cgcgctgaac
     1201 agatgaacga tattgttatc gcggaagtga agacagaaaa aaccgatcag cattcacctt
     1261 ttgtcgaaaa aatgaaaatg atgcatatcc atccgcagga gttcagcaaa tatacgatag
     1321 gagtcagtga aatatatgac ggaatgaaga agaacaaaat aaaaccaacc ttgttgaaaa
     1381 tttcgaaaat aaaaagagag gctcaatttt atgaataacc caataatgat tctgatcggg
     1441 tttgtttata accttttggt ggcgatgatt ctgattcgat ttatctatta cccgaagtct
     1501 tccgtacaat cacatgtctt tacctttttg gcattcaata ctgtaatttt ctttgttata
     1561 aactttttaa gcagcacgga aatgggcatc gggatgggtt tcggactttt cgccgttttt
     1621 tcattgctgc gatatcgtac cgatccttta cctattcggg aaatgactta tctgtttatc
     1681 attgtcgctt tgccgattat gaatgctacg gggattgcag acaataactg ggctggaatg
     1741 gtcattgccg acggattgat tctgttgact atttggattc ttgaaaaggg atggggattt
     1801 cactatgaga tttccaaaac catcatatac gagaaaattg atttaatcgt tcccgaaaaa
     1861 tatgatgaat taaaagccga ccttgaaaaa aggaccggcg taaaaattaa taagattgct
     1921 atcgggaaga tcgattttat gagagatatc gcttatcttg aagttttttc ggatcaggat
     1981 cagaagtaca attggaacag acggatggaa gcggcaactg cttcagacat tgacgatgat
     2041 taacgatgtt tgggagagaa taagtggcac gcaaagtgtt ggttattgat gatacccgta
     2101 atattcagat catgctgcgt gattttctgg aaagtcaggg cttcgaagta gaaatcgccg
     2161 gtgacggaat cgaagccatc gattcgattg aaagatcaca gccggacctg attttgctcg
     2221 atatcatgat gccgaatatg gatgggtatc aatttatcag ccaattgcgg aaaacttccg
     2281 atctgccggt tattatgatt accgcaaaac agcaggaagc ggatatcgtt cacggttttg
     2341 aactcggagc ggatgattat attaccaaac ccttcaagct gagagaactg ctgatgcgta
     2401 tgcgggcggt tttgagacgt tccactccaa gagaagagga aaaaccgatc acaatcatcg
     2461 gcgaccttca attggatcgc agtcgccata aagtcacttt aggggatgag ccggtcgaat
     2521 tgacaccctt agaattcttt gttctcgaca tcctgatgga ttctcaaggc aaagtcgtga
     2581 ggaaagaaga tctgtgcatc cgcttaatcg agaacggctt cagcggatcc gaatcaacat
     2641 taaaaattca cattcgaaat attcgaatga agctccggga tgagcttgat caaccaaaat
     2701 atatcgaaac tgtttttgga atcggatatc gttttctgga aggttcctga tgaaaaagaa
     2761 tattcgaagc aaaatgattt tttcttattt actcattgcc ctgatcacag tcattgtcgt
     2821 ttctttactg gttcgctgga attccggaca atccttgatg aatatggttg tagaacagca
     2881 aacagccaat atgaaggaag cggtccaaac ttactacgaa gaaaacggaa cattggacgg
     2941 cttttttgat cgttatgtcc gaaacggctt cgccagcggt ttgccggcac cggaagcggt
     3001 gaattccgac agcgatgcgg tcgcgcaaat gaaaccggtc attaaaccgc aagacaaccc
     3061 acagaattca cctgatattc gcggtcttca cggtttggtc gatgcccaat atcgggcgtt
     3121 gattccgaca atgggttatg aaccggggga gatcgttccc gaaaacatga tgaaggatgc
     3181 ggtccctgtc gaatatgacg gagaaatcat cgcatggatt ttacccgata cacaactcaa
     3241 atttaaactg agcaatgaag agcaactata tctggaaagg acaacgcgag cgatcatatt
     3301 tgcgggaata atcggtgttt gtttggcgat cttaatggga tttctatttt ccaagcgaat
     3361 cttgggaccc gttcagcgat taacaaaggc ttcgaaagct ttagcggcgg ggaatcttga
     3421 tcaaaccgtg cccgttacct ccgaagacga aatcggacaa ttgactcaga cctttaatca
     3481 aatgagtaca gacttggtcc gggccgattt gcaacgaaag agactgaccg ctgatattac
     3541 tcatgattta agcacaccga tacagattat ttcgggatat attgaaatgg ctgaagatga
     3601 aggcattgag ataaactcca accgattaca aatcatcaaa tcggaattgc agcgtctgaa
     3661 acggcttgta gatgatttaa ccacattatc tcaagtggaa gcaggcgggt tggattttca
     3721 atttgaaagg atccgtcccg ccgaaatgct ggaacgggtc tatcagactt acctgcctat
     3781 tttgaaaaag cagaatattc gcttcgtcta tgatgtttcg gaagattgcc ctgatatctt
     3841 agccgatgaa gatcggatga ttcaggtcct ccataatttg attgaaaatg ccttaagata
     3901 cacacccgag aatggcacaa tctcactcaa agcatatttt gatgaattcg tgagaattga
     3961 agttcgagac agcggggcgg gaatcgattc ggaagatttg ccctatgttt ttgatcgttt
     4021 ttatcgtgcc gacaaatccc gaaatgtcaa cagcggcaaa atggggttag gattggcaat
     4081 ttgtaaagcg ctgatcattg cgcaaaaagg aaagatttct gccgaatcag aaggaatcgg
     4141 caaaggaacg acttttctca ttttgtttcc tccgtataac ggaatggagt aaataagacg
     4201 agacgaacct ttaacaaaat gccaggatcg gaaaagaagc gggagacatt aaataaaagt
     4261 cggcgatatt ttccaatatc accgactttt ttctatgact tattaattt
//
LOCUS       DAOMIV010000126         4305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_21147_length_4305_cov_4.132000, whole genome shotgun sequence.
ACCESSION   DAOMIV010000126 DAOMIV010000000
VERSION     DAOMIV010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21147_length_4305_cov_4.132000"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PKV59_04050"
     CDS             <1..596
                     /locus_tag="PKV59_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45958.1"
                     /translation="EIAEKIERSFLVNVAMNSKHEINGVFCGEIRHAHQAGCDFVKQN
                     VMVPVPKQFDIVIGTNSGYPLDLNLYQTVKGISAAARIVKEGGTILIASECSDGIPEE
                     GMFKTLLKETNSPQAFFDKLREPEFLVQDQWQVQILMQILQKAKVCLYSDRLSDEQIR
                     ACHLIPIHSIEEFCRKEGKGKSICIMPEGPLTIPYLT"
     gene            790..1896
                     /locus_tag="PKV59_04055"
     CDS             790..1896
                     /locus_tag="PKV59_04055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HQP45959.1"
                     /translation="MTLKVGMLTSGGDCQGLNSAMRGVGKRLFENDPQTEVYGFLDGY
                     AGLMQSNYRVMKPSDFSGILTLGGTILGTSRQVFKTMNDPLYPNRSDGPTKLEGMIRT
                     YKDLNLDGLVILGGNGTHKTANLLSENGLNVITLPKTIDNDLWGTDMTFGFQSAVNIA
                     TEVIDGIHSTASSHGRVFIIEVMGHNVGWMTLHAGIAGGADVILIPEIPYDPLAVAAA
                     VNKRAREHKRFSIIAVAEGAKTKEEAKMSKKEYAEKIGNLPFLSVAYRLASILDKLTV
                     QEVRCTVPGHFQRGGSPCAYDRVLTTALGVAAADWVIQKKFGVMVGMVNNEIVGIPLK
                     DVAGKVKSVPTNHPLIQSARNIGIYFGDEDLENG"
     gene            1889..3436
                     /locus_tag="PKV59_04060"
     CDS             1889..3436
                     /locus_tag="PKV59_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45960.1"
                     /translation="MAKPLIVAAIDVDSHDVIMKIAGLKSDEPPKIIDEVQRTIPMGA
                     DTYSTGKISQNLLNQTIEVLKTFNKKMEEYKVNTVQAAATSAFREAANQLFVVEQIRR
                     LTGIEVEVLSSGMDSYYHQIALSETIPDFTEMIQTNVLIVNIGSGSIQLTLFNDGKYI
                     NTQNFRLGPLRIRELLGDLERHSTDFGGLMTEYISGELNYYKTFGPKRATYQNLIVLG
                     GSSRYIKHVAGWTSKKQGQIKKEDFESFFEKLKSGNPNDLPRLSQVPTEQETLMLPTC
                     IVIDEVLQFTGIDQLYISDVDLTTGMLYKMANELQKYRFKRDPDINRIESAIYLANRY
                     RTDKKHSQHVSHLAVGLFDLTVKIHGLPKIARVYLEMAAILHNIGKYVSMENDGVRSY
                     NIIQSNELIGLNERELEIVALTACFHNGRVHPNDPMLQKLTSDEKLMTLKLISILALA
                     NSLDAGHQQKIEIIKTRVEDDRLEIFISSDKDFTLELWNFNKHTGIFQALFGLHPEIK
                     LLKSKKI"
     gene            3500..>4305
                     /locus_tag="PKV59_04065"
     CDS             3500..>4305
                     /locus_tag="PKV59_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45961.1"
                     /translation="MYGDFFDNDQSFLSRIDDLIDFNNVDLPAIEEFPSRFINRELSW
                     LEFNERVLEEARDSENPLFERLNFLSITGSNLDEFFMIRVASLKDQYNAGYTLPDPAG
                     LLPEKQLELISDRTHEMVRKQYSTLLRSIVPALEKNRIEIINSSELNPTQKDYVKRYF
                     ESTVFPVLTPLAVAAGSPFPLIANKTINLIVRVEIPTAKNQKGQRMPFAVVQIPAILP
                     RLLQLPSEEGDGQFILLEDVVSEHINRLFSGVKIGTISCFRIMRNADLDI"
BASE COUNT         1284 a          851 c         1006 g         1164 t
ORIGIN      
        1 tcgagatagc cgagaaaatt gagagatcat ttttagtgaa tgtcgcaatg aattccaaac
       61 atgaaatcaa tggagttttt tgcggagaaa ttcgtcatgc acatcaggcc gggtgtgatt
      121 ttgtcaaaca aaatgtgatg gtgccggtcc ccaaacaatt tgatattgta atcggaacga
      181 attcgggtta cccgctggat ttaaatcttt atcagacggt taaaggaatc agcgcagctg
      241 ccagaattgt gaaagagggc ggaaccattc tgattgcttc ggaatgcagt gacggaattc
      301 ccgaagaagg aatgttcaag acgcttctca aagagaccaa ttcacctcag gcttttttcg
      361 ataagctccg ggaaccggaa ttccttgttc aagaccaatg gcaggtccag atcctcatgc
      421 aaattttaca gaaagcgaaa gtctgtcttt actccgacag attatcggac gaacaaattc
      481 gggcttgtca cttgatcccg attcattcta tcgaggaatt ctgccgcaag gaaggaaaag
      541 gaaaatccat ttgtattatg cctgaaggtc cgttgacgat tccttatttg acataaaaaa
      601 ataaaataat catctgaagt tgattatgaa aagcgaaaaa gaaaacaaaa acgaattagg
      661 aggaacgatc aatcaacccg atcgttttat tacaaagatt cggaaatcct gaggttcggc
      721 agaatttaat atctgaattt ataatttata tagagacgaa ttgttccttt agatgaaagg
      781 ggtaaaaaaa tgacattaaa agtcggaatg ttaaccagcg ggggggattg ccaagggctt
      841 aattctgcca tgcgcggggt cggaaaaaga ttattcgaaa atgaccctca gaccgaggtt
      901 tatggctttt tggatgggta tgccggtttg atgcagagta attatcgtgt gatgaaaccc
      961 agtgattttt ccggtattct gactttgggc ggaacgattt tggggacatc ccgtcaagta
     1021 tttaagacga tgaatgatcc tttgtatccc aaccgcagtg acggaccgac caaattggag
     1081 gggatgatac gcacctacaa agatctaaat ttggacggat tggtgatcct cggtggaaac
     1141 ggtactcata agactgccaa cctgctttcc gaaaatggat taaacgtcat aacattgccc
     1201 aaaaccatcg ataatgattt atggggaacc gatatgacct ttggtttcca gagtgcggtg
     1261 aatatcgcaa cggaggtgat tgacgggatt cactccactg catcctccca tgggcgggta
     1321 tttattattg aagtgatggg gcataatgtc ggctggatga ctttgcatgc cgggatagcc
     1381 ggcggtgccg atgttatatt gattccggaa atcccttatg atccactggc tgtagctgca
     1441 gcggtgaaca agcgtgcccg tgagcataaa cgcttctcga ttattgcggt agcggaaggt
     1501 gcgaaaacaa aagaagaagc aaaaatgagc aaaaaggaat atgcggaaaa gatcgggaat
     1561 cttcctttct tatccgttgc ttaccgattg gcttcgatct tggataagtt gacggttcag
     1621 gaagtccgct gtacggttcc cggacatttc caacgcggtg gttctccttg tgcttacgat
     1681 cgggtattga cgaccgcttt gggtgtcgct gccgctgatt gggtcattca gaaaaaattc
     1741 ggtgtcatgg tcgggatggt caataatgag atcgtcggaa ttcccctgaa ggatgttgcc
     1801 ggaaaagtga agtctgtacc aacgaatcat cccttaattc aatcggcgcg aaacatcgga
     1861 atttatttcg gagatgaaga tttagaaaat ggctaaacca ctgatcgttg cggcaattga
     1921 tgtcgattcc catgatgtga tcatgaaaat tgccggattg aaatccgatg aaccccctaa
     1981 gataatcgat gaggtacaac gtaccattcc gatgggcgcc gatacgtatt caaccggaaa
     2041 aatcagtcaa aatttattga atcaaacgat cgaagttctc aagacattca ataaaaagat
     2101 ggaggaatac aaagtcaata cggttcaggc tgccgccacc agtgcctttc gggaagccgc
     2161 taatcagctt tttgttgttg aacaaatccg cagattgacc ggcattgagg tggaagtcct
     2221 ttcgagcgga atggattcat attatcatca gatcgcctta tcggaaacga ttcctgattt
     2281 tacggaaatg atccaaacca atgttttgat cgtaaatatc ggatccggca gtattcagct
     2341 gactttattt aatgacggga aatacatcaa tactcagaat tttcggctgg gaccgttacg
     2401 aattcgtgaa ctgctgggag atctggaacg gcactcgacg gatttcggcg gattaatgac
     2461 cgagtatatt tcgggagaac tgaattacta taaaaccttc ggaccgaagc gggcaactta
     2521 tcagaatctg atcgttttag gcggatcatc ccgttacatt aagcatgtag ccggttggac
     2581 gagcaaaaag caagggcaaa tcaagaagga agattttgaa agcttttttg aaaaattaaa
     2641 aagcggtaat ccgaatgatc tcccgcgact ttctcaggta cctaccgaac aagagacatt
     2701 gatgctgccg acttgtatcg ttattgatga agtattgcag ttcaccggaa tcgatcagct
     2761 gtatatttcg gatgtcgatc tgacaaccgg aatgctttac aaaatggcga acgaactgca
     2821 aaaatatcgg tttaaacgag atccggatat caaccggata gagtcggcaa tttatttggc
     2881 gaatcgttat cgcacggata aaaaacattc gcaacatgta agtcatttgg cggtcggtct
     2941 gtttgatttg acggtcaaga ttcacggatt gccaaaaatt gcgcgtgtat atcttgaaat
     3001 ggctgctata ttgcataata tcggcaagta tgtcagcatg gaaaatgacg gtgtgcgaag
     3061 ttacaatatt atccaatcca atgaattgat cggtctgaat gaaagagaac tcgaaattgt
     3121 tgcgctaacc gcttgctttc ataacggaag ggttcatccg aatgatccca tgctgcaaaa
     3181 actgacttcg gacgaaaaat taatgactct caaactgatt tccattttag ctttggcaaa
     3241 ctctttggat gcgggtcatc aacagaaaat tgaaattata aaaacgcggg tggaagacga
     3301 tcgcttagaa atatttatca gttccgacaa ggattttact ttggagttat ggaatttcaa
     3361 taaacatacc ggcatattcc aggcattatt cggattgcac cccgaaatta aattactcaa
     3421 atcaaagaaa atataatttt acttttttca gtcgactttc gaatttgacc ggcttcatca
     3481 aaaaaggaac agaagacaga tgtacggaga tttttttgat aatgatcaat cattcctatc
     3541 gcggatagat gatttgattg attttaataa tgtcgattta cccgccatcg aagaatttcc
     3601 gagccgtttt atcaaccggg aattgagctg gctggaattt aatgaacggg tgcttgaaga
     3661 agcgagagat agcgaaaatc cgctgtttga acgtttgaat tttctttcca tcaccggttc
     3721 caacctggac gaatttttta tgatccgagt cgcttcgctt aaggatcagt acaatgccgg
     3781 ctacacctta ccggatccgg ccggcttatt gccggagaaa caactggaat tgatctcgga
     3841 tcggactcac gaaatggtcc gaaaacagta ttctactctt ttgcgatcga tcgtccccgc
     3901 attggaaaag aaccgaatcg aaataatcaa ttcgtccgaa ttgaatccga cgcaaaaaga
     3961 ttatgttaag cggtatttcg aatcaaccgt tttcccggtg ctgacgccgt tggcggttgc
     4021 tgccggctcg cctttcccat taattgccaa taaaaccatt aatttgattg ttcgggtaga
     4081 aatcccgacc gcgaagaatc aaaaaggtca gaggatgcca tttgccgtcg tacaaattcc
     4141 cgcaatcctt ccccgtcttt tacagctgcc ttccgaagaa ggggacgggc aattcatctt
     4201 gttggaagat gtcgtatccg aacatatcaa tcgattattc agcggagtaa aaatcggaac
     4261 gatttcctgc ttccgaatta tgcgaaatgc ggatttggac attga
//
LOCUS       DAOMIV010000127         4245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_21550_length_4245_cov_4.267542, whole genome shotgun sequence.
ACCESSION   DAOMIV010000127 DAOMIV010000000
VERSION     DAOMIV010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21550_length_4245_cov_4.267542"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKV59_04070"
     CDS             complement(<1..474)
                     /locus_tag="PKV59_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP45962.1"
                     /translation="MKITLKQIAREAGVSQATVSRLVNQNTGVSSELEEKVTNAAKKL
                     GVELPRKSSAVKSDQTKNKVIGLLVTDLSNPYFQVIMRGVIDEARILNYGVQIFETYE
                     DIRSEEEAIKTIERFKLDGAILSASRISENKLINFHKNTKIPIALMNRYVKEPEIC"
     gene            complement(658..1584)
                     /locus_tag="PKV59_04075"
     CDS             complement(658..1584)
                     /locus_tag="PKV59_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQP45963.1"
                     /translation="MAYLKIISSTFSLFVAIAVGFFAGKKDFLTESSTTELSRFVIRI
                     LFPIYLFRSAVTYINKENFLSAPLYTFLNVIVMIAMWFISRQIVKKANISPDRIPMFE
                     FTSLAGNTGFFGLTFATLLFGSEEIMAAILYDFGGSLVIFMILVPFLLDNNHIHHPIN
                     FLKEPNVIAILSGLILGILGVKIPEFLAPSFNLLSQITLPLALILCGAQLASIKISKE
                     FHFSQVLYVILIKMILMPLLLFVIVLWIPLDGQMKQFLVVDAALPSGISMVNFAKTYH
                     QDSDFAAVAVFATTAFSVIWLPFLLIVLSSIF"
     gene            1728..>4245
                     /locus_tag="PKV59_04080"
     CDS             1728..>4245
                     /locus_tag="PKV59_04080"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HQP45964.1"
                     /translation="MVSNLPIKNHFNKILACCFLLPFLIFIAILAFEKVYPFGSNSLL
                     YSDLSDQFIGFLSELARIYKGEGNLFYTFHAAGGINFFPTFVHYCSSPLYLLLIFFPE
                     ERMIDAVTVIILLEISLSGLTFGYYLGKRFNCLNKPIIVPFAVCYALSGYSIAFYFLF
                     SFQNAIILLPLVCWQIEKIISRDQWGGCVILYFLLIVSNYYCGYMASLFSCFYFFIRI
                     ISTDLSISRKEKIRKTGLILISIILATGLSAFLWLPLFRALKENADLIGQPFPIHHFN
                     FSAFELFPKLFIGIGDGVKANLPYIYCGILPLISGCLFFLNKQIPKKTKIFSGALYII
                     LILSFTDQILNFIWHGFDYPNWFHYRFSFVFSFLLLTCSFEFLLKMHINNLEIILCGV
                     FLIFGLLSFHQIYPDRIKLYQIFINLFFIVVYSLFLLIVNRGKRNRYIPYFLWIFCSI
                     ECFLNGILLYKENSVVLADRNSYLDYHRKYADLIDRYSSKIPGDFYRIEKTDRRSMSN
                     DSIELGYNGLSLYTSILNKKFQALTQSLGITSINYWIRYEGSTIFTDSLFGIKYLFSD
                     YALNRDYDEIESFVWSNPYAFPMFFYSDSALNSVGSSAYENPVLFQEAILEGIAGETG
                     RFYESVPIQDISLDNLVYSDTEKDQVRVLNPAQPSSITIRFKTSRISPYYLFIPQVIG
                     GYELLINGTSVDIDSYMSNGIQYLLNLYPYLKERESSVIQINFHRQDWQEFQLPSVYE
                     FDIDRFSILTKKIQEDAPQVIQTGFNSFKILLNQEANNLILVSSIPYESGWQVFLDGK
                     RIKYDDLLSGLLCIKNIPEFAQEIQLKFIPPGFRIGLVISI"
BASE COUNT         1278 a          769 c          735 g         1463 t
ORIGIN      
        1 gcagatttcc ggttctttca catagcgatt catcaaagcg attggaattt ttgtattctt
       61 atggaaatta atcagcttat tttctgaaat acgcgaagcg gataaaatcg caccgtctaa
      121 tttaaaacgc tcgattgttt taatcgcttc ttcttcggat cttatatctt cataagtttc
      181 gaatatttga actccgtaat ttaaaattct ggcttcatcg ataacgcccc tcataattac
      241 ttgaaaatag ggattggaaa gatcggtgac caacaatccg ataactttat tcttggtttg
      301 atcggatttc actgccgagc tttttctcgg caactccacg ccaagttttt ttgccgcgtt
      361 cgtgaccttt tcttctaatt ccgaactaac accggtgttt tgattaacta aacgtgacac
      421 cgttgcctga gaaactcccg cttctcgtgc gatttgttta agcgttatct tcatttttcc
      481 tttgaaataa ttacagaaat ttcaaatgtc actaattata aatattataa gggttttgaa
      541 gaaaaaacca atctgaacaa agtcatgaat gaattatgac aggttgggac ttttaagctt
      601 ttttatgaaa tatttacgat aagggtgaaa taatttctga aataattaat tgatttatta
      661 aaatattgaa gacaaaacga tcagtagaaa cggaagccat ataaccgaaa aagcggtagt
      721 cgcaaaaaca gctaccgctg cgaaatcaga atcctggtga tatgtctttg cgaaatttac
      781 cattgaaatt cccgatggta aagcagcatc aactacaaga aactgcttca tttgcccatc
      841 caaaggaatc cataaaacaa tgacaaacag gagtaatggc atcagaatca ttttgattaa
      901 tatgacatat agtacttgag aaaaatggaa ttcttttgat attttaatgg atgccagttg
      961 tgctccgcat aaaatcaaag ccaaaggtaa agtgatttgg ctcagcaaat taaaggaagg
     1021 cgctaagaat tcaggaattt tcaccccaag tattcccaat atcaaaccac ttaaaatggc
     1081 aataacgttc ggctctttca gaaaatttat aggatgatgt atatgattgt tatctaaaag
     1141 aaatggaacg agaatcataa aaattactaa cgatccaccg aaatcgtaaa gaatcgcagc
     1201 cattatttcc tcggaaccaa agagaagtgt ggcaaaagtc aacccgaaaa agccggtatt
     1261 cccagcaagg gatgtgaatt caaacatcgg gattctatcc ggcgaaatat ttgctttttt
     1321 cacgatctgt ctggaaataa accacattgc tatcatcaca atcacattta agaatgtata
     1381 aagcggagcc gaaaggaaat tttccttatt gatatacgtc acagcggacc gaaacaaata
     1441 aatcgggaat aagattcgaa tcacaaatcg tgaaagttcg gttgtggagg attcggttag
     1501 aaaatctttc tttccggcga aaaaaccgac tgcgatggct acaaaaagtg aaaaagtgga
     1561 agatattatc ttaagataag ccatttcgtg ggagaatttt aacacgaatt gcggtctttt
     1621 tacgatttgc gaattgctct gtaaaatgga tttctcggat attattaatt tagtgattgc
     1681 tatcagccgc attttaatag tttgtgccat atgaaaagaa taaagaaatg gtctcaaatc
     1741 tgccgataaa aaatcatttc aataagattt tagcttgttg ttttcttttg ccctttctga
     1801 tttttattgc catcctcgct tttgaaaaag tttatccttt cggaagcaat tctcttctct
     1861 atagtgattt atcagaccaa tttatcgggt ttttatccga attagccaga atttataaag
     1921 gagaaggaaa tcttttttac actttccacg ccgccggagg gataaacttc ttcccgacat
     1981 ttgttcatta ttgctcaagt ccgctttatc tccttctgat tttctttccg gaagagcgaa
     2041 tgatcgatgc ggtcacagtg attattttat tggaaatttc attaagcgga ttaacctttg
     2101 gatactacct cggaaaacgc ttcaactgtt taaataaacc gataattgtt ccttttgctg
     2161 tttgctatgc tttgtctggg tattcgattg ctttttattt tcttttctca tttcaaaatg
     2221 caatcatttt attgccgctt gtatgctggc aaatcgaaaa aatcatttcg agggatcaat
     2281 ggggcggatg cgtgatccta tactttcttt taattgtgtc taattattat tgcgggtata
     2341 tggcaagtct tttttcttgt ttttattttt tcataaggat catcagtacc gatctgtcga
     2401 tttctcggaa agaaaagatt cggaaaaccg gactcattct catttccatt attctggcaa
     2461 ccggtttgag cgctttttta tggctgccct tattcagagc attaaaagaa aatgctgatt
     2521 taatcggcca accgtttcca attcaccact ttaatttttc ggcttttgag ttatttccca
     2581 agctttttat cggaatcggc gacggagtca aagcgaactt gccctacatt tattgtggaa
     2641 ttttgccatt gatatcaggt tgcctcttct tccttaacaa acagattcca aagaagacaa
     2701 aaattttttc gggtgcactt tatatcattc ttatcctttc atttacggat caaattctca
     2761 attttatctg gcatggattt gattatccca attggtttca ttatcgtttt tcttttgtct
     2821 tttctttctt attgttgact tgttcttttg aatttttgct caaaatgcat ataaataatt
     2881 tagaaattat cctatgcggt gtctttttaa tttttggctt gctttctttt catcaaattt
     2941 atcccgacag aataaagctt tatcaaattt tcatcaatct gttttttata gttgtttatt
     3001 cactattttt actgatcgtg aaccgcggaa aacgaaatag gtatattcct tatttcttat
     3061 ggatattctg ttctattgaa tgctttttga acggcattct cctttataag gaaaattcgg
     3121 tggttctggc ggatcgtaat tcttatttgg attatcatcg caaatatgcc gatctgatcg
     3181 atcgttattc atcaaaaatt cccggagatt tttatcgaat tgaaaaaacg gatcgacgtt
     3241 ccatgtcaaa tgattcaatc gaattgggat ataacggcct ttcgctctat acctcaatat
     3301 taaacaaaaa atttcaagcg ttgactcaat ctctgggcat tacttcgatt aattattgga
     3361 ttcgatatga gggatccact atattcacag attcgctttt tggtattaag tatctctttt
     3421 ccgattatgc tttgaaccgt gattatgatg aaatcgaaag ttttgtttgg tcgaatccgt
     3481 atgcatttcc gatgttcttt tattcagatt ccgcattaaa ttcagtcgga tcttccgctt
     3541 atgaaaaccc cgttttattc caagaagcaa tattagaagg aatagcagga gaaaccggcc
     3601 gattttacga aagtgtgccg atacaagata tatcgctcga taacttggta tattcagata
     3661 cggaaaaaga tcaggtacga gttttaaatc ccgctcaacc ttcttcgatc accattcgat
     3721 ttaaaacttc aaggatttct ccatattatt tgtttattcc acaggtgatt ggcggatacg
     3781 aattattaat caacggaaca tcggttgata tagattccta tatgagtaac ggaatccaat
     3841 atttattaaa tctttatcca tatttaaaag aaagagaatc ctccgttatc cagattaatt
     3901 ttcatcggca agattggcaa gaatttcagc ttccatctgt ctatgaattt gatatcgatc
     3961 gtttttcgat tcttacgaaa aagattcaag aagatgctcc acaggttata caaaccggat
     4021 ttaattcatt taaaattctt ctgaatcaag aagcaaacaa ccttattctg gtttcctcga
     4081 ttccctatga atcgggatgg caagtttttc tcgacggaaa aagaataaaa tatgacgatt
     4141 tactttccgg tttactctgt attaagaata ttcccgaatt tgcacaggag attcaattaa
     4201 aatttattcc gcccggattt cgaatcggtt tagtgatttc tatcc
//
LOCUS       DAOMIV010000128         4227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_21696_length_4227_cov_3.454698, whole genome shotgun sequence.
ACCESSION   DAOMIV010000128 DAOMIV010000000
VERSION     DAOMIV010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21696_length_4227_cov_3.454698"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..604)
                     /locus_tag="PKV59_04085"
     CDS             complement(125..604)
                     /locus_tag="PKV59_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45965.1"
                     /translation="MIGHLGFSYVGLVYLLMLWIPNAIWSKNKPIDYRPDRENRVLLI
                     FERAGQVCCTCCVLIFTDLNITPFSGRSLWLIASFFSMILYELNWIRYFLNEHTEKNF
                     YRSFCGIPVPAASLPVLAFLLLGIYGNVIWLILSSILLGIGHIGIHIRHLRAIEKVL"
     gene            complement(624..1421)
                     /locus_tag="PKV59_04090"
     CDS             complement(624..1421)
                     /locus_tag="PKV59_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQP45966.1"
                     /translation="MKRQKIRKFLLLISFLLFPVTLYYFSPVLIIQGSFSGIVTGSLI
                     LFAIQFVVSLFWGRAFCGWACPGGSLQEYCSQVIDKKVSDKKTDRIKWIIWMPWIFTI
                     IFGFISQGGIKSVNFFYMTDHGISVSGITELVVYLFFVFLITILALTVGKRGMCHSIC
                     WMAPFMILGSRIKNRFGYPSLHLEANSEKCVKCGVCSKNCPMGLNVEEMVRTNTLQND
                     ECILCGGCVDLCAKNVLRLTFKKAEKPNTKKAYILDTTEDPSKTVSI"
     gene            complement(1421..1999)
                     /locus_tag="PKV59_04095"
     CDS             complement(1421..1999)
                     /locus_tag="PKV59_04095"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQP45967.1"
                     /translation="MTQKNNTAYIILGLLTHKDSSGYDLKKEIDLKISRFWNIGYGQL
                     YPTLKMLEEDGSIIGKPEKSEKGPDRIIYSITEKGKKKLTDWLSLPNEKEYVRYEILL
                     KLFFGNLSGPTNTIQRIETFQKQHQEDLQLMQSFKTNLESVFQENADHLYYYLTVLFG
                     EHIYKAYLDWADEALAFINKNLLSEENKKESS"
     gene            complement(2188..2961)
                     /locus_tag="PKV59_04100"
     CDS             complement(2188..2961)
                     /locus_tag="PKV59_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HQP45968.1"
                     /translation="MKKHVKIAIFLSLLITVIAGCSSSNQKASEKKYRVATDPVWPPF
                     EMVDEKTKEITGFDIDLMNAIAENQGFAVEYVNVNFDALIPGVGTCQYDAGASAITIT
                     DERKKQMLFSDPYVNIGQVMVVREDVNDLVNKEDFAGKVIGAQTGTTGAIMAEEWQTE
                     GLVTFRGYDTIDLAYMDLKNGQIDGVLGDNTMAEAYVKSMGGLKIVGDQYSSEELAFA
                     VCKDNKDLLDKINAGLKALKESGKFDEIYKKYFEQSVNK"
     gene            3200..3877
                     /locus_tag="PKV59_04105"
     CDS             3200..3877
                     /locus_tag="PKV59_04105"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HQP45969.1"
                     /translation="MKNSEKKIRIFLIISLITLLIVGFVPAYQEETPIISIDVDALTA
                     KIEAALSKQTDQKIADLELRISSLEEKLKAYETRITTLEKLASLPTITPAPKGTPTET
                     PIPTRTPNPTGYECSTELLSPYYYGQFNPGSEFMFQVRITNTGSKTWGNDVTIKYSDG
                     LKAEVEEHYAYALPVASVAPDESIEISIPMKAPTEKKNDGKYQSYYVLNNGKEDFCEF
                     SYSIYVP"
     gene            complement(3943..>4227)
                     /locus_tag="PKV59_04110"
     CDS             complement(3943..>4227)
                     /locus_tag="PKV59_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45970.1"
                     /translation="DTLTFYNDSQSIDLTKKHVFQINMNRSPDTSIYLMDRENQSLLN
                     TTIKLNLIRNLVPDRFEGDGDGKVVTAFGFIGQFQVVWASQDQLFVGALN"
BASE COUNT         1330 a          927 c          729 g         1241 t
ORIGIN      
        1 ggtaataact cattgaggat catttatcgg aaagaaaagc atccgtaaaa ttttccgata
       61 agccttttca atacagggaa aatggtaaag aaatagtaac tcgcttgctt gtgcaaatcg
      121 attcttacaa gactttctct atcgctctta aatgccgaat gtgtattccg atatgtccga
      181 tcccgagtaa aatgctcgac aaaatcagcc aaataacatt tccgtagata cctaacaaca
      241 agaaagctaa aacgggtaaa ctcgcagcgg gaacgggaat tccacaaaaa ctacgataaa
      301 aattcttttc cgtatgttca ttcaaaaaat agcgtatcca attaagttca tacaaaatca
      361 tggagaaaaa cgatgcgatc aaccataaac ttctgccgga aaacggagta atattcaaat
      421 cagtaaaaat aagaacgcag caagtacagc acacttgtcc ggcacgttca aatatcaata
      481 aaactctgtt ttcccggtcc ggacgataat ctatcggttt attcttactc catattgcat
      541 tcggaatcca aagcatcaat aaataaacca aacctacgta ggaaaaacct aaatgtccta
      601 tcatcagaat gcctgaaaga aaactatatt gaaacggttt tggacggatc ttccgtagta
      661 tccaagatat atgctttctt agtgttcggt ttctccgctt ttttaaaagt caatctcaaa
      721 acatttttag cacacagatc aacacacccg ccacaaagga tgcattcatc attttgcaaa
      781 gtatttgtcc ggaccatttc ttcgacattc aagcccatcg gacaattctt agaacaaact
      841 ccgcatttca cgcacttttc cgaattcgct tccaaatgaa gagatggata tccaaaccga
      901 tttttgattc tacttccgag gatcatgaac ggagccatcc agcaaatcga atggcacatc
      961 ccgcgcttac cgaccgtaag cgccagaatg gtaatcaaga aaacaaagaa aagatagaca
     1021 accaattccg ttatgcccga gaccgaaatt ccgtgatcag tcatgtagaa gaaatttacg
     1081 ctctttattc cgccttgtga gataaaacca aaaattatcg taaatatcca aggcatccag
     1141 ataatccatt ttattcggtc tgttttttta tcgcttacct ttttatcaat aacctgtgag
     1201 caatattcct gaaggctccc gcccgggcaa gcccaaccgc aaaaagctct tccccaaaaa
     1261 agagaaacaa caaactggat cgcaaacaga atcaaacttc ccgtaacaat gcccgaaaag
     1321 cttccttgta taatcagtac gggagagaaa taatacaagg tgacaggaaa taataaaaat
     1381 gaaattaaaa gcagaaattt acgaattttt tggcgcttca tcatgagctc tctttcttat
     1441 tttcttccga tagcagattt ttatttataa atgccaaggc ttcatctgcc caatcgagat
     1501 aagctttata aatatgttct ccgaacaaga ctgtcaggta ataataaaga tgatctgcat
     1561 tttcctgaaa gacgctttcc aaattcgttt taaatgactg catcagttgt aaatcttcct
     1621 gatgctgttt ttgaaatgtc tcgattcttt gaattgtatt tgtcggaccg ctgagatttc
     1681 caaaaaataa ttttaggaga atttcatatc gcacatattc tttttcattg ggtaatgata
     1741 accagtcggt cagctttttc ttcccttttt cagtgatcga ataaataatg cgatccggac
     1801 ctttttccga tttctcaggc ttaccgataa ttgaaccatc ttcttccaac attttcaatg
     1861 tgggatacaa ctgaccgtaa ccgatgttcc aaaaccttga aatcttaaga tcgatctctt
     1921 ttttcaaatc atacccgctt gaatctttat gggtcaacag accgagtatg atataagccg
     1981 tattattttt ttgagtcatt tctcaacctc ttcgaaaatc tgacataact atatcaaatt
     2041 gatatagtta tgtcaagagt gttatcccta aattcgtttc ggatgaataa aaaaggtcgg
     2101 aaagatatca gccgatatct ttccgaccca actgtgggaa agccaaaaac tttcccgatc
     2161 ttccgctcga ttggtgcggc ttttacatta tttatttaca gattgttcga aatacttttt
     2221 atagatttca tcgaattttc cggattcctt caacgctttc aagccggcat tgattttatc
     2281 cagtaaatct ttgttatctt tacagaccgc gaaagccaat tcttcggatg aatattgatc
     2341 gccgacaatt tttaatccgc ccatcgattt cacataggct tccgccattg tattgtcgcc
     2401 caaaacaccg tcgatttgac cgtttttcag gtccatgtaa gccagatcga ttgtgtcata
     2461 gcctctgaat gtaaccaacc cttccgtctg ccattcttca gccatgatag cgccggttgt
     2521 tccggtttga gcaccgatca cctttccggc aaaatcttct ttattcacca gatcattcac
     2581 atcttctctg acaaccatta cctgaccgat attgacatag ggatcggaga aaagcatttg
     2641 tttcttccgt tcatcggtaa tcgtaatcgc agacgcaccg gcatcatatt ggcaagtccc
     2701 gacgcccgga atcagtgcat cgaaattcac gttgacatat tcaacggcga aaccttgatt
     2761 ttcggcaata gcgttcatca agtcaatatc gaaaccggta atttctttcg ttttttcatc
     2821 caccatttca aaaggcggcc aaaccggatc cgttgcgact cggtactttt tctctgatgc
     2881 tttctgattg cttgaagaac atccggcgat aaccgtaatt aataaactga gaaagatagc
     2941 tatttttaca tgctttttca taatattctc ctctttgaat aaaaagctgt tggattaaat
     3001 ttatcggatt ttagcataga aaaaataatc tttataaatt tatgacaaac tttttggggg
     3061 aggacggtag attttgtcct ttatccgatt ttattaattg aattttcaac gaattatttt
     3121 ataaatattt ttgtcaaacc gataaatatg atatgctaag attaatttgg attttacttt
     3181 tgacaaaaag gagtagatta tgaaaaattc cgagaaaaaa atacgaatct ttctgataat
     3241 atcgttgatc acgttgctga ttgtcggctt cgtaccggct tatcaggaag aaactccaat
     3301 catttccatc gatgtggatg ctttaaccgc aaaaatcgaa gcagcccttt ccaaacaaac
     3361 agaccaaaaa atagctgatc ttgaacttcg aatttcttct ctcgaagaga aattgaaagc
     3421 ttatgagacc agaatcacca cactggaaaa attagccagc ctgccgacga tcacacccgc
     3481 gcccaaagga actcccacgg aaacgcccat acccacgcgt actccgaatc cgaccggata
     3541 tgaatgttca accgaactcc tcagcccata ttattacgga cagttcaacc cgggctccga
     3601 atttatgttc caggttcgaa ttaccaacac cggttccaag acttggggaa atgatgtcac
     3661 aattaaatat tcggacggtt tgaaggcgga agtcgaagaa cattatgctt atgcgctgcc
     3721 ggtagcctcg gtcgcgccgg atgaatccat tgagatcagc attccgatga aggctcccac
     3781 ggaaaagaaa aatgacggga aatatcaatc ctattatgtt ctgaataacg ggaaagaaga
     3841 tttttgcgaa ttcagttaca gcatatacgt tccgtaagca ttcgtacgat ccgacgaatc
     3901 ataaaaggga aatgataaga acatttccct tttatgattc gtttaattca acgcaccgac
     3961 aaaaagctga tcttgagatg cccacaccac ctggaattga ccgataaatc caaaagcagt
     4021 aaccaccttt ccgtccccgt ctccttcaaa gcgatccggt acgagattac gaatcaaatt
     4081 taatttgatc gtagtattca aaagcgattg attctctcga tccatcaaat aaattgacgt
     4141 atccggagaa cgattcatgt ttatttgaaa aacgtgcttt tttgtcagat caatgctttg
     4201 cgaatcgtta taaaatgtca gcgtatc
//
LOCUS       DAOMIV010000129         4222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_21731_length_4222_cov_4.334293, whole genome shotgun sequence.
ACCESSION   DAOMIV010000129 DAOMIV010000000
VERSION     DAOMIV010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21731_length_4222_cov_4.334293"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PKV59_04115"
     CDS             <1..451
                     /locus_tag="PKV59_04115"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45971.1"
                     /translation="QPQRISPGKFIDSEGKEIGQHDGIPFYTIGQRKGLKISAKNPLY
                     VIAKDVIRNEIVLGQKEDLRVNKVVADDINWICNEQTVDSFDCFAQFRYKSKAVPVSV
                     RITEEKKMIISLKDDFFDGVACGQIAVLYDTEGEVIGSGRILKTERK"
     gene            451..711
                     /locus_tag="PKV59_04120"
     CDS             451..711
                     /locus_tag="PKV59_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45972.1"
                     /translation="MILPAIIFSFIMATLYACIFHLIRGGNLFTLFIYIVVSNIGFFG
                     GQYIAELMGIKFLTLGTINFGVGTIVSIGLLLIGGWISRPIE"
     gene            764..991
                     /locus_tag="PKV59_04125"
     CDS             764..991
                     /locus_tag="PKV59_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HQP45973.1"
                     /translation="MSEKLTGTVKWFNTSKGYGFIAVPGQKDIFVHYLDIRSDGFKNL
                     SEGQQVEFIPVESSKGLRATEVHVIDKEKKE"
     gene            complement(1002..2360)
                     /gene="trmFO"
                     /locus_tag="PKV59_04130"
     CDS             complement(1002..2360)
                     /gene="trmFO"
                     /locus_tag="PKV59_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HQP45974.1"
                     /translation="MVKMFDMGSNNQSDLCIIGGGLAGCEAAWTAAQRGVHVILYEMR
                     PQKMTEAHVTGNLAELVCSNSLGSNLIDRASGLLKEELRFLGSLLIDIAEKTKLPAGN
                     ALAVDREAFSIAIENAVLSHPNIQVIRDEVKYIPDDLPVIIASGPLTSESLCKDIVRL
                     TGEENLYFYDAIAPVVYADSIDFKIAFRASRYDFDNSEEGDYINCPFSKEEYDRFVDL
                     LTHAERIELKDFEKDILSGQQKFFEGCLPIEILAARGKDALAFGPLRPTGLKDPHTGH
                     RPRAVLQLRQDNLAGDLYNLVGFQTNLRYSEQERVFRNIPGLENAVFARFGQMHRNTF
                     LSSPGLINEHLQLISDPRIFFAGQLAGVEGYMGNIGTGLAAGLNAAAFIHGDPLPLFP
                     IETMLGSMIHYLANSEKKNFQPVKANFGILPPLENPPRSKQERGRQYAIRSLDTLKKL
                     RS"
     gene            2582..3991
                     /locus_tag="PKV59_04135"
     CDS             2582..3991
                     /locus_tag="PKV59_04135"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45975.1"
                     /translation="MYQRQKQELRQQTSAHLAQTMTILSMSADEIREKIASELSVNPA
                     LELTDERICPTCHRLLPDNGICPNCCTLKSEDEESIVFVSPRDDFAVENYAVHSELSH
                     EPDMDSAQHEDLPTYVLRQIGPDLEGDEKMIAAYLLTSLDEDGFITSSVLEIAQYYHV
                     LPSKIEEVIQLIQHADPLGVGSRNPQEALMVQIQHLSGLIDIPDKTLDVIRDSYDLLI
                     HGKYREISKIHDLTTKQINTIEKFIGENLNPYPGRAYWGEKGIDNNVDNNTYHFPDIL
                     IYYLNDNPKNPLVVEIIMPINGNLRVNEFFKRESKNAPEDKVEEWKNDIDRASLLIKC
                     LHQRNNTMVRLMEVLVDLQGNFIRYGDSNIVPITRAQISKLLDVHESTISRAVANKTV
                     MLPNKKVVPLSAFFDRSLNVRTIIKDLVKEEKKPLTDAKIVEVLTGMGIDIARRTVAK
                     YRAMEGILPAHMRNSANLR"
     gene            3997..>4222
                     /locus_tag="PKV59_04140"
     CDS             3997..>4222
                     /locus_tag="PKV59_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45976.1"
                     /translation="MENISIENSFEVKTICDSFLYSCITPAFIWAVQEGRLISANPMG
                     VEFLNSFRSSVDNAELSDLIRNWKEIPDEDH"
BASE COUNT         1293 a          815 c          892 g         1222 t
ORIGIN      
        1 gcaaccgcaa cggatttcgc cgggaaaatt tatcgattcg gaaggaaaag aaattgggca
       61 gcatgacggg attccttttt atacaatcgg acagcgaaaa ggattaaaaa tatccgcaaa
      121 gaatccgcta tatgtcatcg caaaagacgt aattcgtaat gaaattgttt tgggtcaaaa
      181 agaagatttg agagtaaata aggtggttgc cgatgatatt aattggatct gcaacgaaca
      241 aacggttgat tccttcgatt gttttgccca gttccgatat aagtcaaagg cggtgcccgt
      301 ttctgttcga ataaccgagg aaaagaaaat gatcatttct ttaaaagatg acttctttga
      361 cggagtggct tgcggacaaa ttgcggttct atatgatacg gaaggcgaag tcataggcag
      421 tggtagaatt ttgaaaacgg aaagaaaata atgatactac ccgcaattat tttcagtttc
      481 ataatggcta ctttatatgc gtgtatcttc catttaatca gaggcgggaa tctttttacc
      541 ctttttattt acattgttgt ctctaacatc ggcttttttg gcggtcaata tattgccgag
      601 ttgatgggaa taaaattttt aacactcgga acgattaatt tcggagtggg aacaattgtc
      661 agtatcggct tactattaat tggcggatgg atcagcaggc cgattgagta aatgcgatat
      721 ccgtcggact ttttatcaag taagaatttc aggagatcat attatgagtg aaaaattaac
      781 cggcacagtc aaatggttta atacgagtaa gggatacgga tttatagcgg ttccgggtca
      841 aaaagatatt tttgttcatt atttggatat ccggtcggac ggatttaaaa atctgtcgga
      901 aggtcaacag gttgaattta ttccggtaga aagttcaaaa ggactacggg caactgaagt
      961 tcatgttatc gataaagaga aaaaagaata attcaaccgg ctcacgatcg aagttttttt
     1021 aatgtatcta aagagcgaat tgcatattgc cttccccgtt cctgctttga tcggggaggg
     1081 ttctctaagg gaggtaatat gccgaaattc gccttcaccg gctgaaaatt ctttttctct
     1141 gaatttgcca agtaatgaat catcgagccg agcattgtct ctatagggaa aaggggtaac
     1201 ggatctccgt ggatgaatgc ggctgcattt aatcccgcag ccaaaccggt tcctatattt
     1261 cccatatatc cttcaactcc ggccagttgc ccggcaaaga atattcgcgg atcggaaatt
     1321 aattgcagat gttcattgat caagccgggg gaagaaagga atgtattccg atgcatttga
     1381 ccaaagcggg caaaaacggc attttccagc ccgggaatat ttcgaaatac tctttcttgt
     1441 tcggagtagc gaagatttgt ttgaaaacct accaggttat ataaatctcc cgcgagatta
     1501 tcttgtcgta actgcaaaac cgcccgtgga cgatgcccgg tatgtggatc tttcaatccg
     1561 gtcggacgaa gaggaccaaa tgccagcgca tctttccctc tggctgccaa tatttcaatc
     1621 ggtaaacagc cttcgaaaaa tttctgctga ccggaaagga tatctttctc aaaatctttt
     1681 aactcaatcc gttcggcatg agtgagcaaa tcgacaaacc gatcatattc ttcttttgag
     1741 aatggacagt ttatataatc tccttcttcg gagttgtcaa aatcataacg agaagctcga
     1801 aaagcaatct taaaatcgat cgaatcggca taaacaaccg gggcgattgc atcataaaaa
     1861 tagagattct cttctccggt caagcggaca atatctttac ataacgattc ggaagtcaac
     1921 ggtccgcttg cgatgattac cggtaaatcg tcgggaatat acttcacttc atcacggatt
     1981 acctgtatat tcgggtgact caaaacagca ttttcaatcg cgatcgaaaa tgcttcacga
     2041 tccacagcta atgcattccc tgcgggcaat tttgtttttt ccgcaatatc gatcagaagt
     2101 gagcccaaaa aacgaagttc ttctttcaat aagccggaag cgcgatcgat caggtttgat
     2161 cccaaagaat tggaacaaac caattccgcc agatttccgg ttacatgggc ttcggtcatt
     2221 ttttgtggtc tcatttcgta aagaatgaca tgaactcctc tttgtgcagc agtccatgca
     2281 gcttcacaac cggctaagcc cccgccgatt atacataaat cggattgatt attgcttccc
     2341 atatcgaaca tttttaccat ggattttctc cttgatcgcg ttgtaataag aatattcaat
     2401 aaacggaata ttcagaatta aaaatcatag cgactccttt taaactcttt tgaagaatac
     2461 ttgagtctgg tacaattctt gtgctgtggt ataaaacttg cacctatagt ttaaacgaat
     2521 ggtttctgaa taataacgaa tacggatttc tgaaacttgc cggaataatg aggaataaat
     2581 tatgtatcaa cgtcaaaagc aagaattacg tcagcaaact tcagcacatt tagcacaaac
     2641 catgacaata ctcagtatgt ctgccgatga aattcgcgaa aaaatagcca gtgaattatc
     2701 agtaaatccg gctttggaac tgactgatga aagaatttgt ccgacctgtc atcgtttgct
     2761 tcccgacaat ggtatttgcc ctaactgttg caccttaaaa tccgaagatg aagaaagcat
     2821 tgtttttgtc agcccgcgtg atgattttgc ggttgaaaat tatgcggtcc attcggaatt
     2881 atctcatgaa ccggatatgg attccgcgca gcatgaagat ttgccgactt atgttttacg
     2941 acaaataggt cccgatctcg agggtgatga aaaaatgatc gcggcttatt tactgacaag
     3001 tcttgatgaa gacggattta taacttcctc tgtattggag attgctcaat attaccatgt
     3061 acttccttca aaaatcgagg aggttattca attaatccag catgcggatc cccttggtgt
     3121 cggttcgcgc aatcctcaag aagctttaat ggttcaaatc cagcatttat ccggattaat
     3181 cgatatcccc gataagactt tggatgttat tcgtgattct tatgatttat taatccacgg
     3241 gaaataccgg gagatttcaa aaatccatga tctcacaacc aaacaaatta atacgattga
     3301 aaaatttatc ggtgaaaatc tgaatcctta tcccgggcgt gcttattggg gggaaaaagg
     3361 aatcgataat aatgtagaca ataataccta tcactttccg gatattttaa tttattactt
     3421 gaacgataat ccaaagaatc cgcttgtagt agagataatt atgccgatca acgggaatct
     3481 gagagtgaat gaatttttca aaagggaatc gaaaaatgcg cccgaagata aggtggagga
     3541 gtggaaaaat gatattgacc gtgcttcgct gcttattaaa tgtctccacc aaagaaataa
     3601 tacaatggtg cgattgatgg aagttttggt cgatctccaa gggaatttca ttcgatacgg
     3661 agacagcaat attgttccga ttacccgcgc acagatatcg aaattgttag atgttcatga
     3721 atctacaatc agtcgagcgg ttgcgaataa aacagttatg cttcccaata aaaaagttgt
     3781 ccccttgtca gcattttttg atcgaagtct gaatgtccga acaattatca aagatttggt
     3841 caaagaagaa aagaaaccac tgactgacgc aaaaattgtc gaagtattaa ccgggatggg
     3901 aatcgatatc gcccgcagaa cggttgccaa atatcgtgcc atggaaggca ttttaccggc
     3961 tcatatgaga aacagcgcta atttgagata agacgaatgg aaaatatctc gattgaaaac
     4021 tcttttgaag tcaagaccat ttgcgactca tttttatatt cttgtatcac tcccgctttt
     4081 atttgggctg ttcaagaagg acggttgatt tcggcaaatc ccatgggagt tgaatttctc
     4141 aattcattcc gttcttcggt tgataacgcg gaactttctg atctgattcg caactggaaa
     4201 gagatacccg atgaagacca tc
//
LOCUS       DAOMIV010000130         4210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_21827_length_4210_cov_3.426233, whole genome shotgun sequence.
ACCESSION   DAOMIV010000130 DAOMIV010000000
VERSION     DAOMIV010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21827_length_4210_cov_3.426233"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1163
                     /locus_tag="PKV59_04145"
     CDS             186..1163
                     /locus_tag="PKV59_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HQP45977.1"
                     /translation="MKRKSIFLSLIVIILICGCSNRKSPDSSSQSDRSISQSNNPYLA
                     DYQGESSNYNEAAYAPKMASLESQSNSEVTRLIVKTADMTIEVQDTSQALEKISNIAT
                     QFGGFVVQSNTWKESEYEEQSLLYGEITIRVKAENFDETIRMIENLTPNSEKNVLSKV
                     ISGNDITSDYIDTKSRLTSLENTRTKLNEIMATATTAEETLAVYKEIADIESQIEVLK
                     GQIKYMEESAALSSVTVKIHPIQPEKTIEVKEWSISEILKGAVQDLIDSAQDFGEWLI
                     YFVISVLPFLIIIFVPIYFIVRACSRRRERNKQKKADSKKTTDQKDNQP"
     gene            1256..3751
                     /locus_tag="PKV59_04150"
     CDS             1256..3751
                     /locus_tag="PKV59_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQP45978.1"
                     /translation="MAARISEKEKEQLKHAGEIVYENITPEKDPVYTPIPSFVSDKVI
                     SLLSARGIRFLYQHQLLALEAEFNKNNVILSTGTASGKSLCYQIPIMEYLLRDPDSTM
                     LLIFPTKALTQDQRKNLASLIPEKIQEIAIYDGDTPPYQRAAIRRNARVILTNPDMLH
                     NGMLPFHPSWARFFENLKLVVLDEAHIYRGVFGAHTANVIRRLIRVHKHYTQSSLSPQ
                     FILCSATLSNAKQLAEKLVGSEVTEFSQDTSGNGVREIFFLNPPIVDEALHLRAGSIY
                     TAAKVAEIMEQKNRQVLLFCQSRQSVESTVRRLRDIGINADGYRSGYLSKERRKIENG
                     LKEGSNRCVVATNALELGMDIGGMDSILSIGYPGSIASYYQRMGRAGRNHQPSDFIMI
                     ASQNPIDQYLMKHPEYITEQKAEPALIDPDNLLILYQHLQCALSEVPFSFSESYGGLT
                     IDETQELLESFCSMGIARKSSGHFYWLESSSPQHTISLRNSGLDRISIVSQDFNGKKV
                     PVGDIDRPSAYWMVHEGAIYFHNGESYRIDELNLDENTAFARPIPAEYTTEADKKTTI
                     LTKNVLMKNSVQNADLSAAEVTVTTQVIGYKKRDIETNQILGSYPLDLPQEKLETKAF
                     ILNLSNQFREELRINSHWTNDPNQYGPNWFKIRKTILERDHFQCQLCSVSGTEQTLHV
                     HHIIPFRNFTNPVDANQPENLTTLCHTCHDRVEAAVKMRSGLTGFASAFRQISALFLE
                     CDANDLNVISDPACAEYDGKATIYLYETIPGGIGLSQNIFYNFIKIVSAVKDLIENCD
                     CEDGCPGCVGPAGEIGIGGKEEAFAIASGLLLV"
     gene            3773..>4210
                     /locus_tag="PKV59_04155"
     CDS             3773..>4210
                     /locus_tag="PKV59_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45979.1"
                     /translation="MGDILSQLRQLGVTLGMPENQDFVNERHSSTLDALKITFPDGIV
                     GKNEFGSYFINRKIYPMPMMHGCIELNDPLILTDNLLSILGIDSIDKEKVLAMDTETS
                     GLSVASSAFVFMIGMGYFYEDSYIIDQLILPDLGEEKAFLAQIE"
BASE COUNT         1328 a          837 c          871 g         1174 t
ORIGIN      
        1 attccctgac ggatcaaaaa gagatctacc cttaatggga aaagaccaac ttgccgagct
       61 tatcatggag gaaataaaca aaatcataat gaaacaatga caagaaaggt tatatccttt
      121 ggaactttga ctccattaat ttcgttttcg ggtaaaagaa gttatagaaa tattatggag
      181 taaaaatgaa aagaaaatcc atctttttaa gcttgatagt aatcattctg atctgcggat
      241 gcagcaacag aaaatctccg gattcctctt cccaaagtga tcgctccatt tctcaatcaa
      301 ataatcccta cttagctgat tatcaaggag aatcttcgaa ttacaatgaa gctgcatatg
      361 cgcccaaaat ggcttcgctt gaaagtcaat caaattcgga agtgactcga ctgatcgtaa
      421 aaacagcgga tatgacgatc gaagtccaag ataccagtca ggcgttggaa aaaatcagca
      481 acatcgccac acaatttggc ggatttgtcg ttcaatcgaa tacttggaaa gaatcagaat
      541 atgaagaaca atcccttctt tatggcgaaa ttacaatccg cgtaaaagct gaaaacttcg
      601 acgaaactat ccgaatgatt gaaaatttga caccaaattc cgaaaaaaat gtgttgagca
      661 aggttatttc gggaaatgat attaccagcg attatattga tacaaaatca aggctgacca
      721 gtcttgaaaa caccagaaca aaattaaatg agatcatggc aactgccacc accgccgaag
      781 aaacattagc ggtttataaa gaaatagcag atatagaatc tcaaattgaa gttctgaaag
      841 gacaaattaa atacatggaa gagagtgctg ctctttcttc cgtcactgta aaaattcatc
      901 cgattcaacc cgaaaaaaca atcgaagtca aagaatggtc aatttccgaa attttaaagg
      961 gcgctgtcca agatctaatt gattcggcac aagattttgg agaatggcta atttactttg
     1021 ttatttctgt cctaccattt ttaatcatca tatttgttcc gatctatttt atcgttcgag
     1081 cttgctcgag aaggagagag aggaataaac agaaaaaagc agacagcaaa aaaacgaccg
     1141 atcaaaaaga taatcaaccg taagatcaat acaaatccgc tcattttttg aagatcccga
     1201 aggaaaattt tgggatcttt tgtttgttga tcccggtgat aaaatcattg gtaaaatggc
     1261 tgcacgaatt tccgaaaaag aaaaagaaca gttaaagcat gcgggtgaaa ttgtttatga
     1321 aaacatcaca cccgaaaaag acccggtata tacgccgata ccttccttcg tttcggataa
     1381 agtaatctcc ttgctttcgg cgagaggcat tcgattttta tatcaacatc aattgttggc
     1441 attagaagct gaatttaaca agaataatgt aatcctttcg acgggaaccg ccagcggaaa
     1501 aagtctttgt tatcaaattc cgatcatgga atatctatta agggatcccg attctacgat
     1561 gttattaatc tttccgacga aagccttgac gcaggatcaa cggaagaact tggcatcctt
     1621 gattccggaa aaaatacagg aaattgccat ttatgacggt gatacgccgc cataccagag
     1681 agccgcaatt cgtagaaatg cgcgtgttat tctcaccaat ccggacatgt tacataacgg
     1741 aatgcttcct tttcatcctt cgtgggcacg gttttttgaa aacctaaagc tggttgtttt
     1801 ggatgaagca catatctatc gcggggtttt cggcgctcac acggcgaatg tcattcgaag
     1861 gttaatcaga gttcacaaac attatactca gtcttcttta tctcctcagt ttatcctctg
     1921 ttcggcaact ttatcaaacg ccaaacagtt ggcagagaaa ttggtcggct ccgaagtgac
     1981 agaattctct caagatactt ccggaaacgg agttcgggaa atattttttt taaatcctcc
     2041 gatcgtcgat gaagcgcttc atcttcgagc cggttctatc tatactgcgg caaaagtcgc
     2101 ggaaataatg gaacagaaaa atcgccaagt cttactgttc tgtcaatccc gccagtcggt
     2161 cgaatctact gtgcgaagat taagggatat cggtatcaat gccgatggat atcgttcagg
     2221 ctatttatcg aaagaacggc gaaaaatcga gaacggattg aaagaaggct caaatcgttg
     2281 tgtggtcgcc accaatgctt tagagctggg gatggatatc ggcggtatgg acagtattct
     2341 ctcgattggg tatcccggat cgattgcttc atattatcag cggatgggaa gagccggcag
     2401 aaaccatcaa ccctccgatt tcatcatgat tgcatctcaa aatccgatcg accaatatct
     2461 gatgaagcat ccggaataca ttaccgaaca aaaagcggaa ccggcattaa ttgatccgga
     2521 caatcttttg attttatacc aacatcttca atgtgcttta tctgaagttc ctttttcttt
     2581 ttccgaatcc tacggcggtc tgacaattga cgaaactcag gaattgttag agagtttttg
     2641 ttcgatggga atcgcccgaa aaagttccgg acatttttat tggcttgaat catcttctcc
     2701 ccaacatacg atttctttga gaaacagcgg attagatcga atttcgattg tctcacagga
     2761 tttcaatggg aaaaaagttc cggtcggtga tatcgatcgt ccttcagcat attggatggt
     2821 tcatgaagga gctatttatt tccataacgg agaaagttat cgcatcgatg agttgaactt
     2881 agatgaaaat acagcatttg cccgcccgat cccggctgag tatacgaccg aagcggataa
     2941 aaaaacaacc attttgacaa agaatgttct tatgaaaaac tctgtacaaa atgcggattt
     3001 atcggctgcg gaagtcacgg tcactacgca agtgattggt tacaaaaaaa gagatatcga
     3061 gacaaatcag attttgggaa gttatccttt agatttaccg caagaaaaat tagagaccaa
     3121 ggcatttatt ctgaatttga gcaatcagtt tcgcgaagaa cttcgaatca acagccattg
     3181 gacaaatgat cccaatcaat atgggccgaa ttggtttaaa attcgcaaaa cgatcctcga
     3241 acgagaccat tttcaatgtc agctttgttc tgtttccgga accgaacaaa ccctccatgt
     3301 tcatcatatc attccgtttc ggaactttac gaatccggtt gatgccaatc aaccggaaaa
     3361 tctgacgacc ctttgtcata catgtcatga tcgagtcgaa gcggccgtga aaatgcgcag
     3421 cggattaacc ggctttgctt cggctttccg ccagatttca gctctcttcc tcgaatgcga
     3481 cgccaacgat ctcaatgtga tcagtgatcc tgcttgcgcc gaatatgatg ggaaagcgac
     3541 aatttatctt tatgagacaa tcccgggagg gataggcctt tcacaaaata tcttttataa
     3601 ctttatcaaa atcgtaagcg cggtgaaaga tttaatcgag aactgtgatt gcgaagacgg
     3661 ttgcccggga tgtgtcggtc ctgcaggaga aatcggcatc ggcggaaaag aagaagcttt
     3721 tgcaatcgca tccggtttgc ttttggtctg acctgtttgt tacaattaat atatgggtga
     3781 tattctttct caattgaggc agttgggtgt aacacttgga atgccggaaa accaagattt
     3841 tgttaatgag aggcattcaa gcacacttga tgcgcttaaa attacctttc cggacggtat
     3901 tgtcggtaaa aatgaattcg gttcatattt tatcaaccgt aaaatttatc caatgccgat
     3961 gatgcacgga tgcatcgaac tgaatgatcc gctcatttta accgacaatt tattatcgat
     4021 tttgggaatc gattcaattg ataaggaaaa agttttagca atggataccg aaacgagcgg
     4081 tttatcggta gcttcttccg catttgtttt catgattgga atgggctatt tttatgaaga
     4141 cagttatatc attgaccagc tgattttacc cgatttaggt gaagaaaaag cctttttagc
     4201 tcaaattgag
//
LOCUS       DAOMIV010000131         4179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22040_length_4179_cov_3.489573, whole genome shotgun sequence.
ACCESSION   DAOMIV010000131 DAOMIV010000000
VERSION     DAOMIV010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22040_length_4179_cov_3.489573"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PKV59_04160"
     CDS             complement(<1..222)
                     /locus_tag="PKV59_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45980.1"
                     /translation="MNRINMHNLAWLEDPEIFAVNRIAAHSDHDLIDEAGNIIPPFSL
                     NGKWDFLYYEKPDDVDFEVIFDSNHPADFK"
     gene            complement(238..1056)
                     /locus_tag="PKV59_04165"
     CDS             complement(238..1056)
                     /locus_tag="PKV59_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP45981.1"
                     /translation="MKRKFRVSRIFLYLFLIIVSFICVFPFYWMIVGSTNTPIQIIAG
                     KMTFGSHFVENFHTLFNNYNMLRILMNSLKISGISVILILLIGGMAGYGFQMYKSNLK
                     EKLYGFFLLTMMVPFATLMIPLFKIVTKLNLLDTHFAVIIVSAVSAFMIFFFRQSFVN
                     YPTEILEAARVDGAGEFRIFFNIFVPSMKSTFAAAAIYSFITSWNSYLWPLIVLQSND
                     KKTTTLLISMMSSSYTPEYGVIMLAIVVATLPTIIIFFAFQKQFVQGMLGSVKQ"
     gene            complement(1053..1982)
                     /locus_tag="PKV59_04170"
     CDS             complement(1053..1982)
                     /locus_tag="PKV59_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP45982.1"
                     /translation="MGKKLSMKGWLFISPALILLAVFLVYPIIYSFYLSFTSTKGIIS
                     SFVGFKNYIRLTKDPMFLLALKNTGIILVIQVPIMLISALILANILNDRKLAGRGIFR
                     TAIFLPSVTSLIAYTILFKMLFSFDGIINRLLLSTGIVDHPLEWLTTPKLAVFVLILA
                     MLWRWTGYNTVFYLSAMQNISYEIYEAAEIDGASKTQTFLRITLPLLKPMILFTTIMS
                     TIGTLQLFDEPMNLSQGGVTAATMGPGNCFLTLSVYIYNLCFKYVPNFGYAATVSYVI
                     VFIIAALTVLQFWVGQSNEEKDAKKRLEKEGKA"
     gene            complement(2053..3321)
                     /locus_tag="PKV59_04175"
     CDS             complement(2053..3321)
                     /locus_tag="PKV59_04175"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQP45983.1"
                     /translation="MKKTFVCLLVIALFATVFGAVSAENSDGKITVWAWDPSFNIAIM
                     KEAAARYEAINPDVKIDIVEMAKADVEQKLNTILAAKTTEGLPEIVLIEDYNAQKYLQ
                     YYPGSFADLTTKFNYDDFAAYKVGVMTLDGKVYGVPFDSGVVGFFYRSDILAEAGFKA
                     EDLKNITWDRFIEIGKTVKEKTGKYMLGFQNTDGGIMRIMMQSAGKWYFDEEGKPALI
                     GNQALVEAFELYKKIVDSGIAKPTNNWNEWVGTINTGESASVTSGVWIVGSIKAAKDQ
                     SGLWDVAPVPRLSTVDSKNASNLGGSSWYILDKSADKDAAIDFMQKIYAGDKDFYQTI
                     LINNGAVGTYLPAATGEAYSTPDEFFGGRAIYEDLSAWMKEIPPVDYGIYTYEADAAI
                     MAQMEAVCAGTMTIDDAIKAAEAQLENQIQ"
     gene            3572..>4179
                     /locus_tag="PKV59_04180"
     CDS             3572..>4179
                     /locus_tag="PKV59_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP45984.1"
                     /translation="MATMRDIAKAAGVSPATVSRVLNHDPTISVTTATKLRILNVAEE
                     LEYVKKNGRKASAKERLNIAIVDWYSEADLAEDPYYLYLMTAVEKYCASQNINTFRVV
                     NVNGVYLNSVDLKVDGMIAIGRFLISEVEQLSKITSNIIFLDSSPDEERFDSIMVNTK
                     LGTKQALDYLFSLGHRKIAFIGGEVVGNNREKTLDSRKDMFVS"
BASE COUNT         1220 a          894 c          899 g         1166 t
ORIGIN      
        1 cttaaaatca gctggatgat ttgaatcaaa aataacttca aaatcaacgt catcgggttt
       61 ctcataatac aaaaaatccc atttcccatt taaagaaaac ggcggaataa tatttcccgc
      121 ttcgtcgatc agatcatgat ccgaatgggc tgcgatacga ttaacggcaa aaatttccgg
      181 atcttccagc catgcgaggt tgtgcatatt tattcgattc atatcttcaa tcgcccctta
      241 ttgtttgacg gaaccaagca taccttgcac aaactgcttt tggaaggcaa agaaaataat
      301 gatggtcggc agagtggcaa caacgatggc gagcatgatg actccgtatt cgggtgtata
      361 tgaggaggac atcatggata tcaggagcgt tgtcgttttc ttatcattgg attgcagtac
      421 gatcaatggc cacaggtagg aattccagct ggtgataaaa gaatagattg ccgccgcggc
      481 aaaggttgat ttcatcgaag ggacaaaaat attaaagaaa atccgaaatt cacccgcccc
      541 atccacgcgt gccgcttcta agatttccgt cggataattt acgaaactct gccggaagaa
      601 gaaaatcata aacgcgctga ctgcggaaac gatgatgacg gcaaaatgag tatccaataa
      661 gttcagtttg gtaacaattt taaaaagcgg gatcatcaag gtggcaaagg gaaccatcat
      721 cgtgagcaga aaaaaaccat aaagtttttc tttcaggttg gatttataca tttggaagcc
      781 atatcccgcc atcccgccga tcaataggat caggatcaca ctgattccgc tgattttcaa
      841 tgagttcatc aagatacgta acatattgta attattgaat aacgtatgga agttttcaac
      901 gaagtgcgat ccgaaggtca tttttccggc gatgatctga atcggcgtat ttgtagaacc
      961 gacaatcatc caataaaaag gaaaaacgca aataaaagaa acaatgatca gaaataaata
     1021 cagaaaaatg cggctcactc gaaattttct tttcatgctt ttccttcctt ttccaaacgt
     1081 tttttggcat ctttttcttc attgctttgt ccgacccaaa attgcaggac ggtcagggca
     1141 gcgataataa agacgatgac gtaagaaacc gttgcggcat atccgaaatt ggggacatat
     1201 ttaaagcaca aattgtagat gtatacgctt aaggtcagga agcaattccc gggccccatc
     1261 gttgccgcgg tcacccctcc ttgcgaaagg ttcatcggct catcgaacaa ttgcagtgtg
     1321 ccgattgttg acataattgt cgtgaaaagg atcatgggtt tcaataaagg caaagtgatt
     1381 cgcaaaaagg tctgcgtctt actggctccg tctatttccg cagcctcata tatttcatag
     1441 gatatatttt gcatagccga aaggtaaaac accgtgttat atccggtcca tctccaaagc
     1501 atggccagta ttaatacaaa aacagctaat ttgggcgttg tcaaccattc caggggatga
     1561 tcgacgattc ccgtggaaag gagcagccgg ttaataatcc cgtcaaaaga aaaaagcatc
     1621 ttaaataaga tggtataagc aattaaggat gtaacggaag gcagaaaaat ggctgttctg
     1681 aaaatgccgc gtccagccaa ttttcgatca ttgagaatat ttgccagaat taaggcgctg
     1741 attaacataa tcggcacctg tatcaccaag ataattcccg tattttttag tgccaacaaa
     1801 aacatgggat cttttgtcaa tcgaatgtaa ttcttaaatc ccacaaaaga actgatgatg
     1861 ccttttgtgg atgtaaaaga caggtaaaac gaatatatga tcggatatac cagaaaaacc
     1921 gctaaaagta tcaatgccgg actgataaac aaccagcctt tcatgctgag cttttttccc
     1981 attttgtatt gatcctttat ttaaagtgat tgccacccac cggcgaggtg agtggcaatc
     2041 caaagaatta tgttattgaa tttgattttc aagctgtgct tcggctgctt taatggcgtc
     2101 atcgatcgtc atggtgccgg cgcagacagc ttccatctga gccataattg ccgcatcggc
     2161 ttcataggtg tagattccgt aatcgaccgg aggaatttcc ttcatccaag cgctcagatc
     2221 ttcgtagatc gctctgccgc cgaaaaattc atccggagtt gaataagctt caccggtcgc
     2281 ggcgggcaga taggtgccta cggcaccgtt gttgatcaga atcgtctgat aaaagtcctt
     2341 gtcgccggca taaatcttct gcatgaaatc aatcgcggca tctttatcgg ctgatttgtc
     2401 gaggatgtac cagctcgaac cgccgaggtt ggatgcattt ttactgtcaa cggtgctcag
     2461 tcgcggtacc ggcgcaacat cccataagcc gctctggtct ttggctgcct taatggatcc
     2521 gacaatccaa acaccgcttg tcacggatgc gctctcaccg gtgttgatgg ttccgaccca
     2581 ttcgttccag ttgttggtgg gtttggcaat tcccgaatca acaatctttt tatacagctc
     2641 gaaagcctcg accaatgcct gattgccgat caatgccggt ttgccttctt cgtcaaaata
     2701 ccattttccg gcggattgca tcataatgcg cataataccg ccgtccgtat tctggaagcc
     2761 caacatatat ttgccggtct tttcttttac ggttttgccg atttcgataa atcggtccca
     2821 agtgatattc ttaagatctt ccgctttgaa gccggcttcc gccaagatgt ccgaacgata
     2881 gaaaaatccg actacaccgg aatcaaacgg aacaccgtag acttttccgt ccagcgtcat
     2941 gacgccgact ttatatgcgg cgaaatcatc gtaattgaat ttagtcgtca gatccgcaaa
     3001 tgatcccgga taatattgca gatatttctg agcgttatag tcttcaatta gtacgatttc
     3061 cggcagccct tcggtggttt tggcggccag aatcgtattc aatttctgct caacgtccgc
     3121 ttttgccatt tcgactatat caatcttaac gtcgggattg attgcctcgt agcgggcagc
     3181 cgcttctttc atgattgcaa tattgaaact gggatcccat gcccagacgg taattttacc
     3241 gtctgaattc tcagcgctga ccgcgccgaa aacggtcgca aacagcgcaa ttacaagtaa
     3301 acagacaaaa gtttttttca ttttccctcc tggatttctg aaagtatatt ctttcacaaa
     3361 agcgcttgtg taccttgaat ttaatcgttt tacggcggta ggtcaatatt atttagtaat
     3421 atatatcttg ttacattcgt actaatttac taatcaagag ataaatgttt actaaatact
     3481 ttcgggtagt tatataatac tcataaagat atggacaaaa taaaaaaaac aggtaccata
     3541 tagaacgatt aatgaattcg gagggttttc tatggcgaca atgcgagata tagctaaagc
     3601 tgcaggtgtt tctcccgcta ctgtctcgcg tgttttaaat catgatccga caatcagcgt
     3661 caccaccgcc acaaaattaa gaattttaaa cgttgccgaa gaattggaat atgtaaagaa
     3721 gaacggtcga aaggcttccg ccaaagagcg attaaatatt gcgattgtcg attggtacag
     3781 tgaagcggat ttggcggagg acccgtatta tttgtatttg atgaccgccg tcgagaaata
     3841 ctgtgcctcg caaaatatca atacttttcg ggttgtgaat gtgaacgggg tttatctaaa
     3901 ctccgtggat ttaaaagtcg acggtatgat tgccatcggg cgctttctga tcagcgaagt
     3961 ggaacaatta tcaaaaatta cgtccaatat tattttcctt gattcttctc cggatgaaga
     4021 acgatttgat tccatcatgg taaacacgaa gttgggaacc aaacaagcat tggattatct
     4081 gttttcactc gggcatcgaa aaatcgcctt tatcggcgga gaagtggtgg ggaataatcg
     4141 cgaaaagacg ctggacagcc ggaaagatat gtttgtttc
//
LOCUS       DAOMIV010000132         4174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22081_length_4174_cov_3.686574, whole genome shotgun sequence.
ACCESSION   DAOMIV010000132 DAOMIV010000000
VERSION     DAOMIV010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22081_length_4174_cov_3.686574"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PKV59_04185"
     CDS             complement(<1..991)
                     /locus_tag="PKV59_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP45985.1"
                     /translation="MKKNLFWIFVSVLFFSVFFNGTKVEAISFDKVVYLINGSLGDNS
                     FYDSGQAGIDQLKKDYGVEARTIECGFDAGKYEPSLDAAVHYADVIFVISYGYEDVLK
                     EYADKYPDKIFVNLDTVVENDEHTITSVDYIEEESAFLAGIVAALVTTSQEIPNVNPQ
                     KIIGAVGGDTDPVVQSFIFAYENGAKYIDPEVKNETKYLGDWEDTSKAKQATLQLYDM
                     GADIVFQIAAAAGMGVLQASGERGLYSIGVDTNQNAIVPGHVVASDIKDVGKSILNVY
                     KTIKDGTYKPGEVLSYGLESGTVDVVFENNNDVLPKSIIDKVSDVKQMIINGDL"
     gene            complement(1000..2196)
                     /locus_tag="PKV59_04190"
     CDS             complement(1000..2196)
                     /locus_tag="PKV59_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQP45986.1"
                     /translation="MKKKVLILTADAGFGHRSAANAIASALSKETKFEIETRIENLLD
                     SPKTPAPLRNTQTGYDKIVTESPKFYEFSYESSDKRLPTNVAKIGLSAMLYGPFSKTM
                     KEFNPDVLVNTYPLYQGPAQTWYLLNEDAKKAISKSKKADEDFKSSSKHKHIPMITII
                     TDLVSVHLIWMSTLPDIYCVATHEMKEQIAGFGVDRERILCTGIPVNPVFAEEKRSKR
                     ELREQLGWNPEKTTIIAIGSKRVSNMMDYLDLINHSGFNIQLIMVAGGDETLYQEMQN
                     TTWHVPAKIYNFTKELPVMMLASDIVVTKAGGLVTSESLAASLPMILIDVLPGQEEGN
                     ALYIQENNAGVHAKKPIDILHTLTHWFENDGYGLRKFTENARKIGKPRSSLDIADLIV
                     EKLSES"
     gene            2337..2972
                     /locus_tag="PKV59_04195"
     CDS             2337..2972
                     /locus_tag="PKV59_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQP45987.1"
                     /translation="MEPDKIKTPFSFNKAAIIAIAAYIGVQMIADISSLQIVSIFGLA
                     LDAGTFIYPISFTLRDIAHKILGKEHTKTLIYSAAGINLFMAGYFFFVSVLPVDLSAG
                     GSENWIKVLTPVWRITIASIIAELCSELIDTEIYSWWVEKITRKHLWARVLISNGVSV
                     PVDSLLFAFIAFYGMIPLPAVWEIFIGNVVIKYLVTLVSVPLIYLGKETEQ"
     gene            2996..>4174
                     /locus_tag="PKV59_04200"
     CDS             2996..>4174
                     /locus_tag="PKV59_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQP45988.1"
                     /translation="MKTIRNDLRNIAIIAHVDHGKTTLVDAMLKQAKVFRANQQVEER
                     VMDSNDLERERGITILSKNTAISIPDPETSEMVKINIVDTPGHADFGGEVERVMNMVD
                     GALLLVDAAEGPMAQTRFVLKKALAMGLRVIVVINKMDRKDADPERTLNKIFDLFIDL
                     GATDEQADFPIIYADGIKGKAGYTPELSENLQPLFQTILRKINPPEVEIDQPFQMVVS
                     NIFYDEYRGLGVIGRIHRGKLKAGMNLSRIKPDGTIVPENARYLYTYSGLNKVETQEA
                     SAGEIISIAGLNEISIGDTLSDPNYPEALPVISVEEPTVKMTFGVNSSPFAGREGKFS
                     TSRKLRERLFSELRNNVALRVEETDTTDNFVVSGRGELHLAILIETMRREGYEFQVSR
                     P"
BASE COUNT         1180 a          880 c          853 g         1261 t
ORIGIN      
        1 taagatcccc gttgatgatc atttgtttta catcggaaac cttgtcgata atcgatttcg
       61 gcaaaacatc attattgttt tcaaatacca cgtcaaccgt gccggattcc aagccatatg
      121 aaagaacttc tccaggcttg taagttccgt ctttgattgt tttataaaca ttcaaaatcg
      181 atttccccac atccttaata tccgaagcga ctacatggcc gggaacgata gcgttttggt
      241 tggtatcaac gccgatcgaa tataacccgc gttctccgga tgcctgaagc acacccattc
      301 cggctgcggc ggcaatctga aagacaatat ccgcgcccat atcgtaaagt tgcaaagttg
      361 cctgtttcgc tttggatgta tcttcccaat cgccgagata tttggtctca ttcttcactt
      421 ccggatcgat atattttgcc ccgttttcat aggcaaaaat aaatgattga acgacaggat
      481 ccgtgtctcc accgacggct ccgattattt tttgcggatt gacattcggg atttcctgac
      541 tggttgtaac caacgcagcg acaataccgg caagaaaagc actttcctct tcgatatagt
      601 ccaccgatgt tatggtatgt tcatcatttt cgacgaccgt atccagattg acgaatattt
      661 tatccggata cttgtcggca tactccttaa gaacatcctc atatccgtat gatatgacaa
      721 aaattacatc tgcgtaatga acggcagcgt ccaacgaggg ttcgtatttc ccggcatcaa
      781 aaccgcattc tatggtacgg gcttctactc cataatcttt tttcagttga tcgataccgg
      841 cttgaccgga atcataaaaa gagttatcac ctaaagatcc gttgatcaga taaacaacct
      901 tatcaaacga aatcgcttcg actttggttc cgttgaagaa aacggaaaaa aataaaaccg
      961 acacgaaaat ccaaaataaa ttctttttca ttttttctcc tatgattctg ataatttttc
     1021 tacaataagg tcagctatat ccagacttga ccttggctta ccgatttttc tggcgttttc
     1081 cgtaaatttc cttaatccat atccgtcatt ttcgaaccag tgcgtcagcg tatgcaaaat
     1141 gtcgatcggt ttttttgcat ggacacctgc attgttctct tggatgtata aagcatttcc
     1201 ctcttcctga cccggcaaaa catcgattaa aatcatcggc agggaggctg caagcgattc
     1261 gctggtaaca agcccccccg ctttcgttac aacaatatcc gatgcaagca tcatcacagg
     1321 taactctttt gtaaaattat agatttttgc cggaacgtgc caagtcgtat tttgcatctc
     1381 ctgatagagt gtttcatctc cgccggcgac cattatcaat tggatattga atccgctatg
     1441 gttgataaga tccaaataat ccatcatatt tgagacacgc ttgcttccga tcgcaatgat
     1501 ggtagttttt tccggattcc accccaattg ctctcttaat tccctttttg atcttttttc
     1561 ctcggcaaaa accggattaa cgggaatgcc ggtgcataga atccgctctc gatcgactcc
     1621 gaaaccggcg atttgctctt tcatttcatg ggtagcgaca caatagatat cgggcaatgt
     1681 gctcatccag atcaaatgaa cggaaacaag atccgtaata attgttatca tcggtatatg
     1741 tttatgcttt gaagatgatt tgaaatcttc atccgctttt tttgatttac tgatcgcttt
     1801 ttttgcatcc tcattgagaa ggtaccaagt ttgagcgggg ccttgataca atggataagt
     1861 atttacaagg acatccggat tgaattcttt cattgttttt gaaaatggac catacagcat
     1921 cgcagagaga ccgatctttg ccacatttgt cggaaggcgt ttatcgctgc tttcataact
     1981 gaattcataa aatttcggag attccgtaac aattttatcg tatcccgttt gagtatttcg
     2041 gagaggtgcc ggtgtcttgg gtgaatccaa taaattctcg atccttgttt caatctcaaa
     2101 ttttgtttct ttcgacaatg cgcttgcaat tgcatttgct gcacttctgt gcccgaatcc
     2161 ggcatccgcg gttagtatta aaaccttttt tttcatccgt tcattatcct tttcagggat
     2221 tttcttattt tgtcttcagt ttaccagaaa aatataacct attatcgatc ggtcatcatt
     2281 attttttgac cggaaattgc cggcaaaccc aaaactcatt ataatccacc gttggtatgg
     2341 aaccggataa aataaagacg ccattctcat ttaacaaagc ggcaattatt gcgattgcgg
     2401 cttacatcgg cgttcaaatg atcgccgata tcagcagctt gcaaattgtt tcgatattcg
     2461 gattggcttt ggatgccggt acttttattt acccgatttc ttttacactc agagacattg
     2521 cccataaaat attagggaag gaacatacaa aaacgcttat ttattccgca gcgggaataa
     2581 atttatttat ggccggatat tttttctttg tttccgttct tccggttgat ttatccgctg
     2641 gtggaagtga aaattggatc aaagttttaa cgccggtgtg gcgaataact atcgcttcta
     2701 ttattgcgga actttgttcc gaattaatcg atacggaaat ttacagttgg tgggttgaaa
     2761 aaattacaag aaaacatctg tgggcacgcg ttttgatctc caacggagtg agtgttcctg
     2821 ttgactctct gctttttgcg tttatcgctt tttatggaat gatcccgtta ccggctgtat
     2881 gggagatttt tatcgggaat gtcgtaataa aatatctcgt tacgcttgtg agtgtacctt
     2941 taatctattt aggaaaagag acagagcaat aaactctgat ttgaattata aaaaaatgaa
     3001 aacaattaga aatgaccttc gaaatatcgc gattatcgct catgtcgatc atggaaaaac
     3061 cacacttgtc gatgcaatgc tgaaacaagc gaaagtattt cgtgccaatc aacaggttga
     3121 agaaagagtg atggattcca atgacctcga gcgagagcgc ggaattacca ttctgtcaaa
     3181 gaataccgcc atctcgatac cggaccccga aaccagtgaa atggtaaaga taaatattgt
     3241 cgatacaccc ggccatgcgg atttcggagg agaagtcgaa cgggtcatga atatggtcga
     3301 cggtgcactg ttgttagtgg atgccgcaga aggaccgatg gctcaaactc gtttcgtttt
     3361 aaaaaaagca cttgctatgg gtttgagagt aattgttgtg attaataaaa tggatcgaaa
     3421 agatgccgac cccgagcgaa cattaaacaa aatatttgat ttgtttattg atttgggagc
     3481 cacggatgag caagccgatt ttccgatcat ttacgcggac ggaataaaag gaaaagccgg
     3541 atatacgccg gaattgtccg aaaatttaca gccgctgttc caaacaattt taagaaaaat
     3601 caatccgccg gaagtggaaa tcgaccagcc gtttcaaatg gtggtcagta atatatttta
     3661 tgacgaatat cgcggattag gggtcatcgg aagaattcac cgcggaaaat taaaagccgg
     3721 tatgaattta tccagaataa agccggacgg tactatcgta ccggaaaatg ccagatatct
     3781 ctatacttac agcggattga ataaagtcga aacgcaggaa gcctccgcag gtgaaattat
     3841 ttctatcgcg gggcttaatg aaatttccat cggggatacc ctatccgatc cgaattatcc
     3901 ggaagcatta cctgttattt ccgtggaaga accgaccgta aaaatgacat tcggtgtaaa
     3961 ttcctctcct ttcgccggga gagaaggaaa attcagtaca tccagaaagt tacgggaacg
     4021 tcttttttcg gaattgcgaa acaatgttgc attgagagtc gaagagacag atactaccga
     4081 taattttgtt gtctcgggtc ggggagagct tcatttagct attcttatcg aaacaatgcg
     4141 cagagaagga tatgaatttc aagtttccag accc
//
LOCUS       DAOMIV010000133         4141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22319_length_4141_cov_3.251346, whole genome shotgun sequence.
ACCESSION   DAOMIV010000133 DAOMIV010000000
VERSION     DAOMIV010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22319_length_4141_cov_3.251346"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="PKV59_04205"
     CDS             <1..991
                     /locus_tag="PKV59_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQP45989.1"
                     /translation="ADELSYDQYNILSATSLGPRFAADNLLTAWLKIKEPDRCHGFAG
                     FNYPETGAPEAEDLLKQAQEFHALGFDGIKMMDGKPGIRKKIGKPLDDPLYDPMFDYL
                     EQQNWPVLYHVNDPIEFWTWELMPEWAKAMGDRVFYGNGEYPSKDTIEAEAIHVVEKH
                     PRLRIIFAHFFFIADDLEKAAGYFERFPNLMFDITPGWEMFESFANRYEESRDFFRKY
                     ADRIIFGTDTISDHWRTTVGNLRRVLETDDRFVSFEENCHGLNLDEKTLRQIYIENYH
                     KFLPAKPAEIKIDEIIAYADRLSGRIAAEAPGETPAILAEIKNCRQELDQLRD"
     gene            1001..2353
                     /locus_tag="PKV59_04210"
     CDS             1001..2353
                     /locus_tag="PKV59_04210"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45990.1"
                     /translation="MKLTVIGGGGVRSLFLAKSIALEARELNIDELVLMDTNDQKLEI
                     FGSLAMEAAIRLAPHLKVSLTTDAREAVRDADYVITTIRVGEDEARVADEKIALDLHL
                     LPQETTGACGFSFAMRSIPVLADYCGLIRDIAKPTVQVFNFTNPAGLVSQALRDAGFD
                     FTYGICDAPAGLLSQIADLENVSTDHLDMDCYGLNHLSWFPAVRLDGKDITQELIRDD
                     RLYHETDMKFFEPALAQSYQALFNEYLYYYYYREQAVENIRNAKQTRGQLIEQVNRQM
                     ILDFSEHPHLSFEERLKIFEHYYGIREDAYMASETGKKREKTFRFDLTAPDNGGYAGV
                     ALNFIRIRQQGNPGRMILSVPNRGSLPFLEQNDVAELSCIVDSDGVHPDPPPNFHPVA
                     CELIRRMKLYERWAAEAIRCRNFDLSVRALQIHPLIESYSLAKSLATSFFKLNEPWIK
                     "
     gene            2370..3341
                     /locus_tag="PKV59_04215"
     CDS             2370..3341
                     /locus_tag="PKV59_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQP45991.1"
                     /translation="MSFVAGIGRANIDLLYSGLSHIPREGEEVYSKDFDLQLGGGPVA
                     TMINLQRLGVPVKVLTFLGKDMFSAFAEQRLHEYGIEAINLYGGGKIPVNISTAMITG
                     RDRTFVSYNQLQQTTADQLKIAESEIEGAKIVEMQPGFLELYRKLKQKGTLLVLDMGW
                     EDDLSFEKYRDYFELADYYTPNLKEALYLTGTDNPLDALIILSQWFTKPIVKIGDQGC
                     LIKDEEGIWQIPHIDEFESTDATGAGDAFLSGFIYGLYHEADFRTAILYGNIMGGKAV
                     TEVGCLTASLSEQELLHYADRYLNRIARISEKSHYTSISPVNPSSSI"
     gene            3381..>4141
                     /locus_tag="PKV59_04220"
     CDS             3381..>4141
                     /locus_tag="PKV59_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45992.1"
                     /translation="MRKYAGLIKIILLVWVIGSLSACTLPSESQIVSTPYDPVSDLVK
                     TIQSMSSRTPYPTGTPYPTHTPYPTYSPEEIATFYARNIPAVTPTVRILRETAGQNVI
                     LVNPGLWSRDLSKYAFCEDRFAVKIGYRQPYFAYAVGNEVSKSQFLLVNLMVWNISDS
                     TIPLIFHKQFKAVGTYRGEKAEYISYGPAGISASNRWGVPFLSSQAIPPGVEMETWLG
                     FDVNPHATDWKLVFEADDSSDFSCRFSLDMPQPEFS"
BASE COUNT         1108 a          847 c         1044 g         1142 t
ORIGIN      
        1 ggcagacgaa ttgtcttatg accaatataa tattttaagc gcgacctcat tgggtccaag
       61 atttgccgca gataatctgt tgactgcatg gctgaaaatt aaagagcccg accgctgtca
      121 cggatttgcc ggtttcaatt atccagagac gggtgctccc gaggcggaag atttgttgaa
      181 gcaagcgcag gaatttcatg cattgggttt cgacggaatt aaaatgatgg atggaaaacc
      241 ggggatccga aaaaaaatcg ggaagccatt ggatgatccg ctgtatgatc cgatgtttga
      301 ttatcttgag caacagaatt ggccggtact ttaccatgtt aacgacccga tcgaattttg
      361 gacttgggag ctgatgcccg aatgggcaaa agcaatgggc gaccgtgttt tttacggcaa
      421 cggcgaatat ccttccaaag atacgatcga agccgaggct attcatgtgg tcgaaaagca
      481 tccccggttg cgaattattt ttgctcattt tttctttatc gcagacgatc ttgaaaaggc
      541 tgccggatat ttcgaacgat ttccgaatct gatgtttgat atcacacccg ggtgggagat
      601 gtttgaaagt tttgccaacc gttatgaaga gtcgcgggat tttttcagga agtatgccga
      661 tcgaattatt ttcggaacgg atacgatttc cgatcattgg agaacaacag tcggcaatct
      721 gcggcgagtg ctggagacgg atgatcgttt tgtttccttt gaagagaact gtcatgggtt
      781 gaacttggat gaaaaaacat tacggcagat ttatatcgaa aattatcata aattcttacc
      841 cgcaaaacct gccgaaataa agattgatga aatcatagct tatgcagatc gcctttccgg
      901 tcggatcgca gccgaagcac cgggggaaac gccggcgatt ttagcggaaa taaaaaattg
      961 caggcaggaa ctcgatcagt tgagagatta agaggggaat atgaagttaa ctgtcatcgg
     1021 tggtggcggc gtccgatcgc tatttttagc gaagagtatc gcgttggaag cccgggagct
     1081 gaatattgat gaattggttt taatggacac caatgatcaa aaattggaga ttttcggttc
     1141 gctggcaatg gaagcggcga ttcggttagc ccctcatttg aaagtttcgt taactacaga
     1201 tgctcgagaa gcggtccgag atgcggatta tgtgattact acgattcgtg taggcgaaga
     1261 tgaagcccgt gtggcggacg aaaaaattgc cctcgacctt catttgcttc cgcaagaaac
     1321 gacaggcgcc tgcggctttt cgtttgcgat gcgctccatt ccggtgttgg cggattattg
     1381 tggcttgatt cgggatatcg caaaacccac cgtgcaagtt ttcaatttta ccaatcctgc
     1441 cggcttagtt tctcaggcat tgagggatgc cggctttgat ttcacctatg gcatatgtga
     1501 tgcaccggcc gggttgcttt ctcagatagc ggatttggag aacgtttcga cggaccatct
     1561 ggatatggat tgctacgggt tgaatcatct ctcatggttt cccgccgttc ggctggacgg
     1621 aaaagatatt acgcaggaat tgattcggga cgatcggtta tatcatgaaa ctgatatgaa
     1681 attctttgaa ccggcattgg cacaatccta tcaggcatta ttcaacgagt atctgtatta
     1741 ttactattat cgggaacaag cggttgagaa tattcgaaat gcgaaacaga cccgcggaca
     1801 gttgatcgaa caggtcaatc gtcagatgat tctggatttt tccgagcatc ctcatctctc
     1861 atttgaagaa cgtttgaaaa tctttgaaca ctattacgga atccgcgaag atgcctatat
     1921 ggcttccgag acggggaaaa agcgagaaaa gacatttcgc tttgatttga ctgcgccgga
     1981 taacggcggt tatgcggggg tagccctcaa ttttattcgc atcaggcagc agggtaatcc
     2041 gggacgaatg atattaagtg tcccgaatcg cggatcatta ccgtttttag agcaaaacga
     2101 cgtggcggaa ttgagctgta ttgtcgattc ggacggcgtt catccggatc cgccgccgaa
     2161 ttttcatcca gtagcttgtg aattgattcg gcggatgaaa ctttatgaac gttgggcggc
     2221 ggaagcgatt cgctgccgaa attttgacct gtctgttcgg gcgttgcaaa tccatccatt
     2281 aatcgaatct tattcgcttg caaaatcatt ggcaacgtca ttttttaaat tgaacgaacc
     2341 atggataaaa taaatcgggg gaagaaagaa tgagttttgt cgccggaatc ggcagagcaa
     2401 atattgacct tctttattcg ggattatcac atattccgcg tgaaggggaa gaagtctatt
     2461 caaaagattt tgatcttcaa ctgggcggag gaccggttgc gacgatgatc aatttacagc
     2521 gactgggcgt gccggtaaaa gttttaacct tcttgggaaa agatatgttt tcggcattcg
     2581 ccgaacaacg gctgcatgaa tatggcatcg aagcgatcaa tctgtacggc ggcgggaaga
     2641 ttccggtgaa tatttccacc gcgatgatta ccggccgaga tcgaactttt gtttcttaca
     2701 atcagcttca gcaaactacc gccgaccaac tgaaaattgc cgaatcggag atagaaggag
     2761 ccaaaattgt tgagatgcaa cccggatttc tggaattata tcgaaagctc aaacaaaaag
     2821 ggacgctgtt ggttttggat atgggttggg aagatgatct cagttttgaa aaataccgtg
     2881 attattttga actggctgat tattatacgc ccaatctcaa agaagcgctc tatttaaccg
     2941 gcacggataa tccgttggat gcattaataa ttctttctca atggtttacc aaaccgattg
     3001 tcaaaatcgg agatcaagga tgtctgatta aggacgagga aggaatctgg cagatcccgc
     3061 atatcgacga attcgaatcg acggatgcca ccggagcggg agatgcattt ctcagtggtt
     3121 ttatttacgg tttataccat gaggcggatt ttcgcacggc gattttatat ggaaatatta
     3181 tgggaggaaa agcagttaca gaggtgggct gcctgaccgc ttctttgtcc gagcaagaat
     3241 tgctgcacta tgcggaccgc tatctgaatc ggatcgcccg gatcagtgaa aaaagccatt
     3301 atacttcgat ctccccggta aatccgagct catcgatttg ataaaaatga gcataggttt
     3361 ctcgtttata atccttttat atgcgaaaat atgcggggtt gatcaaaata atccttttgg
     3421 tatgggtaat agggagcctt tcggcttgta cgcttccgtc tgaatcccaa atcgtttcga
     3481 caccgtatga tccggtatcc gatttggtga aaaccattca atcgatgagt tcaagaaccc
     3541 cttatccgac cggaactccc tatccgacac acactcccta tccaacctat tctcccgaag
     3601 agattgcgac tttttatgcg agaaatattc cggcggtaac tcctaccgtt cggattttgc
     3661 gagaaaccgc cggacaaaat gttattttgg tgaatccggg tctttggtct cgcgacctca
     3721 gtaaatatgc attttgcgaa gatcgatttg ccgtaaagat cggctacaga cagccttatt
     3781 ttgcttacgc ggtagggaat gaggtatcca aatcgcagtt tttgttggtc aatctgatgg
     3841 tctggaatat ttcagattca accattccgc ttattttcca caaacaattt aaagcggtcg
     3901 ggacttatcg aggagaaaaa gcggaatata tttcttacgg ccccgcagga atcagtgcca
     3961 gtaaccggtg gggagtgcct tttttaagca gccaagcgat tccccccggt gtggagatgg
     4021 aaacatggct cgggtttgat gtcaatcctc acgcaacgga ttggaaattg gttttcgaag
     4081 ccgacgattc gagtgatttt tcctgtagat tttcgttgga tatgcctcaa ccggagtttt
     4141 c
//
LOCUS       DAOMIV010000134         4134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22377_length_4134_cov_4.060064, whole genome shotgun sequence.
ACCESSION   DAOMIV010000134 DAOMIV010000000
VERSION     DAOMIV010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22377_length_4134_cov_4.060064"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PKV59_04225"
     CDS             <1..320
                     /locus_tag="PKV59_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP45993.1"
                     /translation="MVLNTLSTAVMVRLGKTYSNLMVDVQPSNQKLIDRQRRIVAEAC
                     GIDPIRAEELLSQCNREAKTAIVVQKTGISPEEARLILSQNDGFVEKSIESVLSRKDR
                     NSK"
     gene            484..1164
                     /locus_tag="PKV59_04230"
     CDS             484..1164
                     /locus_tag="PKV59_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HQP45994.1"
                     /translation="MNQNLMIGSVFTAGILSFFSPCILPLLPVYFGILTNTSTPENSI
                     EAKKDTGRNLLKTVLFILGIGFVFILLGFGAGSLAPLFSNPLFHRIGGLIIILFGLNQ
                     MGVLRLNFMEKWNAPGFNSSGKNQYLSAFFLGLIVSLGWTPCIGPILASVLILAANSG
                     TAIRGAWYMTFYTLGMAIPFVVIMLFADFLLIRIKKGGQWLPVVRKIGGALLVITGLL
                     LFFNRLNG"
     gene            1178..2749
                     /gene="msrAB"
                     /locus_tag="PKV59_04235"
     CDS             1178..2749
                     /gene="msrAB"
                     /locus_tag="PKV59_04235"
                     /inference="COORDINATES: protein motif:HMM:NF010625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HQP45995.1"
                     /translation="MKNKKFAVLFWMLILILIISGCSSSSKSGENPNEIDLVDLDGQR
                     FKLSEQLGKKVYIKFWASWCPICLEGLPELEKLASESNDFEVVTIVSPGLGKEMDTNE
                     FREWFAGLENNHFRVLLDEGGKIATKYGVHGYPTSFFVSANGTIKVFPGEIKNEMVKA
                     IFKSNLLDGESDPVQENMQKEQEEMEPNSENDAIRGSANSQPSAEAEIYLAGGCFWGV
                     EAYFEKIDGVKEVTVGYANGKTENPSYEDLVYNHSGHAETVHVIYDPSRVTLDQLLQY
                     YFRIIDPTSINRQGNDRGVQYRTGIYYVDESDKAVIETRIAMEQKKYDKKIQVEVTPL
                     IRFDLAEDYHQDYLKKNPNGYCHIDLSLADEPLIDETAYPKPSDSAIKGMLTEEQYNV
                     TQYSGTEKPFTNAYWNTFDDGIYVDIVTGEPLFSSLDKFDSDCGWPSFSKPISDEVVN
                     YHTDTSYGMVRTEVRSRSGDSHLGHVFDDGPAESGGQRYCINSAAVRFIPVSKMEAEG
                     YGYLLKLFDPSKKVD"
     gene            complement(2859..3284)
                     /locus_tag="PKV59_04240"
     CDS             complement(2859..3284)
                     /locus_tag="PKV59_04240"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQP45996.1"
                     /translation="METFWPIILIVISNIFYNITTKSTPADANPFLSLTVTYLVGAIL
                     TFLMFWATATEKGLSSNISRLNWTGYCLGIIIVGLETGYIYLYRAGWNISLGSLVANI
                     LLAISLVIIGALFYHEEISARQLIGMLFCIIGLIFVNKK"
     gene            complement(3330..4028)
                     /locus_tag="PKV59_04245"
     CDS             complement(3330..4028)
                     /locus_tag="PKV59_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQP45997.1"
                     /translation="MVNHTFTDENSIQRNHYPVKIGNLFKLSFGEEVGNSVSHGVSAM
                     ILLFALPACAIYGYQKGGKLLAFGYSIYIISLLLMFLASCLYHAMDFNSKHKQVFRIL
                     DHSAIYIAIAGTFTPVCLSVVGGWLGIVVLICQWVTAIFGILFKSLSNKNLPKASLTI
                     YLVMGWSAILLLPAIIRKQLWGFLAFILLGGLLYTVGTIFYSKKEKGWFHFIWHLFVI
                     GASAAHFVAIIFYI"
BASE COUNT         1251 a          790 c          931 g         1162 t
ORIGIN      
        1 aaatggttct gaacacactt tccacagctg tgatggtacg gttggggaaa acttacagca
       61 acctgatggt ggatgtgcag ccgagcaatc aaaaactgat tgatcggcaa cgccggattg
      121 tagccgaagc ttgcggaatc gatccaatca gggccgaaga actgctctct caatgcaatc
      181 gtgaagcaaa aacagctatt gttgttcaga agacaggaat atctcccgaa gaagccagat
      241 tgattttgtc gcagaacgat ggttttgtgg aaaaatcgat tgagagcgtt ttatcgcgaa
      301 aagatcgaaa ttccaaatga agtgaatctt tagaaagttt tcttatctta aaggttctat
      361 tcttccggaa atagggagaa tgaacctttt tttgaattga tcatctttaa atgataaaaa
      421 gatattaata tttcctttcc ttccgtccga tgctttaaaa taaaatttta gaaaggtttg
      481 attatgaacc agaatctgat gataggtagt gtctttacag ccgggatctt gtcttttttc
      541 agtccctgta ttcttccttt actgcctgtt tatttcggaa tcctgacaaa tacttccact
      601 cccgaaaatt caatcgaagc gaagaaagat accggaagga acctgttgaa aaccgttctg
      661 tttattttgg gaatcgggtt tgtctttatt ttactcggat tcggtgccgg cagtcttgct
      721 ccgttgttta gcaatccctt atttcatcga atcggcggat taatcataat tttgttcggg
      781 ctcaatcaaa tgggtgtgct tcgcttgaat tttatggaaa aatggaacgc accgggtttt
      841 aattcatcgg gaaagaacca atatttaagc gctttttttc tcggattaat tgtcagcttg
      901 ggatggactc cttgtattgg tccgatttta gcttcggtct tgattctggc tgcgaacagc
      961 ggaactgcga ttcgaggagc ttggtacatg actttctata cactcggaat ggcaatcccc
     1021 tttgtggtca tcatgctgtt tgctgatttt ttgttgatac gaattaaaaa ggggggacaa
     1081 tggttgccgg tcgtccggaa aatcggcggg gctttgttgg tgatcaccgg actgttactg
     1141 ttttttaatc gattaaacgg ttgatgaaag gttgtaaatg aagaacaaga aatttgcagt
     1201 tcttttttgg atgttgatct tgattttaat catttccggt tgtagtagta gttcaaaatc
     1261 cggcgaaaac cccaatgaaa ttgatttggt tgacctggac ggacaacggt ttaaattatc
     1321 cgagcagctt gggaagaagg tctatattaa attttgggca agttggtgtc cgatatgttt
     1381 agaaggactg ccggaactag aaaaactggc ttccgaatcg aatgattttg aggtggtgac
     1441 aatcgtgtca ccgggactcg gcaaagaaat ggacacgaat gaatttcggg aatggtttgc
     1501 gggtttggag aataaccatt ttcgggtttt attggatgaa ggcggaaaaa tcgcaacgaa
     1561 atatggtgtg cacggctatc cgacttcttt ttttgtgagc gccaacggga cgattaaggt
     1621 attcccgggt gaaatcaaaa atgaaatggt gaaagctatt tttaaatcaa atctcttgga
     1681 tggagaaagt gatcccgttc aagaaaatat gcagaaagag caggaagaaa tggaacccaa
     1741 ttcagaaaat gatgccattc gtggctcagc aaattctcaa ccttccgccg aagcggaaat
     1801 ttatcttgca ggcggatgtt tttggggagt ggaagcttat tttgaaaaga ttgacggtgt
     1861 aaaagaagtg acggtaggtt atgcgaacgg gaaaaccgag aaccccagtt atgaggattt
     1921 ggtgtataac cactccggtc atgctgaaac ggtgcatgta atctacgatc caagccgagt
     1981 gacattggat cagttgctgc agtattactt cagaattatc gatcccacca gtatcaacag
     2041 acaagggaat gatcgcggag tgcaatatcg taccggcatt tattatgtcg atgaatccga
     2101 taaagcggtg attgaaactc ggatcgccat ggaacagaaa aaatatgata aaaagattca
     2161 ggtcgaagta actcccttga ttcgttttga tttagccgaa gactatcatc aagattatct
     2221 gaaaaagaat ccgaacggat actgtcatat tgatctttcg cttgccgatg agccgttgat
     2281 tgatgaaacc gcttatccga agccatcgga ttccgcgatt aaagggatgc tgacggaaga
     2341 acaatacaat gtcacacaat acagcgggac tgaaaagcct tttaccaacg catattggaa
     2401 tacttttgat gacggaatct atgtggatat cgttaccgga gaaccgcttt tctcttcgct
     2461 ggataaattt gattccgatt gcggctggcc cagcttctca aaacctattt ccgatgaagt
     2521 ggtcaactat cacaccgata catcctatgg gatggtccga accgaagtcc gcagccgcag
     2581 cggcgattct catctgggac atgtttttga tgacggaccg gcagaatcag ggggacagcg
     2641 gtattgcatc aatagtgccg ctgttcgatt tattccggta tcaaagatgg aagcggaagg
     2701 ctacggatat ttattgaaat tgtttgatcc ttcaaaaaaa gtcgattaat cataccgaag
     2761 aggaatgaaa cagacagctt cattcctctt tttagacaat caataggatt catcgatcct
     2821 tttgaattct caataagaat tcaatttttg ttcataaatc attttttgtt gacgaagatc
     2881 aaaccgatga tgcaaaaaag catgccgatc agttgccggg cagaaatctc ctcatgataa
     2941 aaaagggctc cgatgatcac gagagaaatt gccaagagga tatttgctac caaagagccg
     3001 aggctgatgt tccatccagc ccgataaaga taaatatatc cggtttccag gccaacgatg
     3061 atgattccca gacaataacc ggtccaattt aatcggctga tattggaaga gagccctttc
     3121 tctgtggcgg ttgcccaaaa cataagaaaa gtaagtatcg ctcccacaag atatgtgacg
     3181 gtcagagata aaaaaggatt ggcatccgcc ggagtggatt tggtggtgat gttgtaaaaa
     3241 atgtttgaga taacaatcaa aataatcggc caaaaagttt ccacgatttt cccttctctc
     3301 aatgataaag tgtcaaatcc tgtttgtttt cagatataaa aaataatagc gacaaaatga
     3361 gcggctgatg cgccgataac aaacaaatgc cagatgaaat ggaaccaacc cttttctttc
     3421 ttggaataga aaatcgttcc gactgtgtac agcaaaccac ccagaagtat aaaagctaag
     3481 aacccccaaa gctgtttacg aatgatcgca ggcaaaagca agattgccga ccatcccatg
     3541 accagataaa ttgtcagaga agctttcgga agattcttgt ttgaaagcga tttgaataaa
     3601 attccaaaga tagccgtaac ccattggcag attaaaacga ctatacccag ccatcctccc
     3661 acaaccgata gacagaccgg tgtgaaagtt ccggcaatgg cgatatatat cgcactatga
     3721 tccaaaatcc gaaaaacttg tttatgtttg gaattaaaat ccatcgcatg gtaaaggcag
     3781 ctggcaagaa acatcaaaag aagactgata atgtagatac tgtaaccaaa tgcaagcaac
     3841 tttccgcctt tttgatagcc atatatggca caggcaggga gcgcgaataa tagaatcatg
     3901 gccgaaacgc cgtgactgac cgaatttccg acctcttcgc cgaacgataa tttaaaaagg
     3961 ttcccaattt ttaccgggta atggttcctt tgaatcgaat tttcatcggt aaatgtatga
     4021 ttgaccattt tttctcctga tcctattgca agtaaacgga aaagctcaaa atatcaagcg
     4081 aacagttttc cttatttgaa tggtgatatt ctttcttccc ggattttttc atcc
//
LOCUS       DAOMIV010000135         4132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22385_length_4132_cov_4.404709, whole genome shotgun sequence.
ACCESSION   DAOMIV010000135 DAOMIV010000000
VERSION     DAOMIV010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22385_length_4132_cov_4.404709"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /gene="alr"
                     /locus_tag="PKV59_04250"
     CDS             complement(<1..698)
                     /gene="alr"
                     /locus_tag="PKV59_04250"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQP45998.1"
                     /translation="MEKRYDTWVEIDLNAIRHNIQTIALIAGKPVMAIIKANAYGHGM
                     IPVGKAAIAAGCPFVAVARFGEAQELRLAGITTPILVLGYIHPKDIEEAARESIRATL
                     FSYEQMRQYEETLRGTWKKLPVHVKVDVGMGRLGEQAEDGLRFLQNAASSAVFDIEGL
                     YSHFSKADMLEDDATGIQFSRFDRLIREAEKAGLRPRIVHLANSAGAVFHPEICKYDM
                     VRGGIAIYGLDPSDS"
     gene            1066..2460
                     /gene="pepV"
                     /locus_tag="PKV59_04255"
     CDS             1066..2460
                     /gene="pepV"
                     /locus_tag="PKV59_04255"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HQP45999.1"
                     /translation="MKEQIRAKFEAYWDEMMADLAALVAVNSVRGEETTEFPFGKEPA
                     RALSIALRQGERMGFAVENVDNYAGTIDLGDAEEMIGVMAHLDIVPAGDGWTDDPFTL
                     TFKNDRIYGRGVVDDKGPVIAALYGMRIIRDLNLPLKKKIRLIVGTNEEQGSGCMKYY
                     AQHRPVPSCGFTPDADYPLIHGEKGNFWTWLSFPAEETPILEISGGNAFNIVPAECVV
                     LLDGSQVSADELKKSIRTEDTEGYEPQFMHDAKGNIKMVVRGHAAHGSTPEKGVNAIV
                     SAAKILCNAFGERAGKMMHFIAESIGRETDGASLGLKVSDDVSGSLSLNLGRIIYSTE
                     QHRLAIDIRYPVKLSLAEMDANYRKMAEAFGLTYEVTEAAEPHYVPKDSPVVQKLLAV
                     YRQVTGDQTADAFTIGGGTYAKALGKNFVAFGPEFPGSEPMNVHDADEHILKELFLDH
                     CVVCTLGMAALAGE"
     gene            complement(2546..3325)
                     /locus_tag="PKV59_04260"
     CDS             complement(2546..3325)
                     /locus_tag="PKV59_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46000.1"
                     /translation="MFYRKHRKIILPLAVLPIVLILWELYIRIFNVPNYLLPKPYDLF
                     VSLITLFTSGGVWKHIAVTLKEILVGSVIGAAIGLVLGYIMAKVKLIEKMVMPFVIIV
                     QTAPKISLAPLFILWMGLGIESKAALVILVVSFPIMINEVTAIRSIDKNAYDLMKVLK
                     ANRWQVFRHIELPYSMEMLLSGVKLALTQAMTGAVIGEMIGAKAGLGYLLTLGSETYD
                     IKMILNAVILLSVMGLLFYLISEYIERKALYCKELDRSVIG"
     gene            complement(3342..>4132)
                     /locus_tag="PKV59_04265"
     CDS             complement(3342..>4132)
                     /locus_tag="PKV59_04265"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46001.1"
                     /translation="AAAADIVKNVASGNVEFGFPNADAVVAARAQGIPIKVVNTTYQQ
                     GLGAIIFNSNSGIKTPADLKGKKVAVTSLGSANYFQLEAAMKSVGMSIDDVQVEIVGT
                     GAILNALTEGQVDAIVFSKLRTIELNYAGFPAAEIPCDQFLPSFGNVLVAGEKLVNEK
                     PEIVQGFNKALIKGIEYVIDGHVEEAVDYSIEKMAPTFAEKRDVVIKIINEVFIPTLW
                     QSDYTKEHGIGASDVKKWQALIDSSKEYGTIKESFDAKDLLVLN"
BASE COUNT         1096 a          963 c          996 g         1077 t
ORIGIN      
        1 gagtctgaag ggtccaagcc atagatcgca attccgcccc ttaccatatc atatttgcag
       61 atttccggat gaaagacggc tcccgccgaa tttgccaaat ggacaatgcg gggccgcaac
      121 ccggcttttt cggcttcgcg tatcagtctg tcgaaacggc tgaattggat gccggtggcg
      181 tcgtcttcca gcatatcggc ttttgaaaaa tgggaatata gcccttcgat atcaaaaaca
      241 gcggaggaag cggcgttttg gagaaaacga agcccgtctt cagcctgttc acctaaccgc
      301 cccataccga catccacttt gacgtgaacg ggtaactttt tccatgtccc gcgcagcgtt
      361 tcttcatatt gtctcatttg ctcgtagctg aaaagggttg cgcggatact ttcccgagcc
      421 gcttcttcga tatctttcgg atgaatatat ccgagaacca gaatgggcgt ggtaattccc
      481 gccagtcgca gttcctgtgc ttcgccgaaa cgagcgacag ccacgaacgg acaaccggcc
      541 gcgatagccg cctttccgac agggatcatc ccatgcccgt acgcattggc tttgataatc
      601 gccatcaccg gtttcccggc gatcagcgca atggtttgaa tgttatgccg aattgcattc
      661 agatcgattt caacccatgt atcgtatctt ttttccatat tcatctttcc cgattcaata
      721 aaactttttg caacactccg attcggattc gcagataccc gtccggaacg gaatgttcaa
      781 agaagataaa aaattgtgcc ggaatggaga tttcaaattc tcagagaaag gaatgaatcg
      841 attttcttca aaataatcca tattttgatt ttaccattgc gtcggggatt cggaaaaaac
      901 cggcaaggag taattccgac gatgaaacag tagtcctttt ttttgtggga aaacgaatga
      961 aatttttcaa cgtttgagaa acatcccatc aaacgacgtc ggaatcaacc ctttcacctg
     1021 aaattctatt tataatttct atattacacc tacaaggaga cagagatgaa agagcagatt
     1081 cgggctaagt tcgaagcata ttgggatgaa atgatggcgg atctggcggc attggttgcc
     1141 gtcaacagcg ttcgcgggga ggaaacgacg gaatttcctt ttggcaaaga acccgcccgg
     1201 gcattatcga ttgcccttcg acaaggcgaa cgaatgggat ttgcggtgga aaatgtcgat
     1261 aattatgccg ggacgattga tctgggcgat gcggaggaaa tgatcggagt gatggctcat
     1321 ctggatattg tcccggccgg tgacggttgg acggatgacc cctttacgct gacattcaaa
     1381 aatgaccgaa tctacggaag aggtgtcgtg gatgataaag ggccggttat cgccgctctt
     1441 tacggaatgc ggattattcg agatttgaat ctgccgctta agaaaaaaat ccgattgatt
     1501 gtcgggacaa acgaagaaca gggttccggc tgtatgaaat attatgcgca gcaccggccc
     1561 gtcccctctt gcgggtttac accggatgcg gattatccgt tgattcacgg tgaaaaggga
     1621 aacttctgga catggctgtc ttttccggcg gaagagactc ctattttgga aatcagcggc
     1681 ggaaatgcct ttaatattgt tccggcggaa tgcgttgttc ttctggatgg ttcgcaagtc
     1741 tctgcggacg aattaaaaaa atccatacga acggaagata ccgaagggta cgagcctcag
     1801 tttatgcatg atgcaaaagg aaatatcaaa atggttgtcc gcggacacgc cgctcacggc
     1861 agtacgccgg agaaaggggt taacgcgatt gtctccgcag ctaaaatcct gtgcaatgct
     1921 ttcggggaga gggccggcaa aatgatgcat tttatagccg aatcgatcgg cagagaaacg
     1981 gacggcgctt cattgggatt aaaggtgtcc gatgatgtat ccgggagttt gagcctgaat
     2041 ctcggcagaa tcatctattc cactgaacaa caccggctgg cgattgatat ccgctatccg
     2101 gtcaaactca gtttggcgga aatggatgcc aattatcgga aaatggcgga agccttcggt
     2161 ttaacctatg aggtaaccga agcggcggag ccgcattatg ttccgaagga cagtccggta
     2221 gtgcaaaaat tattggcggt ttatcgtcag gtaaccggcg atcagacagc ggatgctttt
     2281 acgatcggcg gcgggactta tgcgaaagca ctcggaaaga attttgtcgc attcgggccg
     2341 gaattccccg gttcggagcc gatgaatgtc catgatgccg atgagcatat tctcaaggaa
     2401 ttattcctgg atcattgtgt cgtctgtaca ctcggtatgg cggcgcttgc cggagaataa
     2461 aaggaaatcg gatccttcat taagaaacga gccttgcgga atttcttttc acaaggctcg
     2521 tctgattttt cttgaaagaa aaatcttaac cgatgacact ccgatcgagt tccttacaat
     2581 acaacgcttt tctttcgata tattcggaaa tcagatagaa caagagaccc atcacactca
     2641 acaaaatcac cgcattcagg atcatcttga tatcataagt ctcgctgccg agcgtcaata
     2701 aatatcccaa gccggcttta gcgccgatca tctctccgat cacggcgccg gtcatggctt
     2761 gagtcaaagc cagtttcact ccggagagca gcatctccat ggagtaagga agctcaatat
     2821 gtcgaaaaac ctgccagcgg ttggctttca gcaccttcat cagatcatag gcgtttttgt
     2881 cgatggaacg gatggcggtc acttcattta tcatgattgg aaaagaaaca accaggatca
     2941 ccaacgccgc tttggactca atgcccaatc ccatccaaag gatgaataaa ggtgccagtg
     3001 atattttcgg ggcggtttgc acgatgatca caaacggcat caccatcttt tcaatgagct
     3061 tcacttttgc cataatgtat cccagcacca aaccgatcgc ggctccgatt accgatccga
     3121 ctaaaatttc ttttaaggta acagcgatat gtttccatac tcctcccgag gtgaacaggg
     3181 tgatcagaga aacgaacaga tcgtaaggtt tgggcagcag ataattcggc acattgaata
     3241 tcctgatgta cagttcccag aggatcaaaa cgatcgggag aaccgccaaa ggaaggatga
     3301 ttttccgatg tttgcgataa aacatgcccg ctctcatttg attaatttaa gaccaataaa
     3361 tctttggcat cgaaagattc tttgattgtt ccgtattctt tggaactatc aatcaaagcc
     3421 tgccattttt tcacatcgga agcgccgatt ccgtgctctt tggtatagtc gctttgccat
     3481 aaagtcggga taaatacttc gttgatgatt ttgatcacaa catcccgttt ttcggcgaaa
     3541 gtgggtgcca tcttttcaat ggaataatct acggcttctt ccacatgtcc gtcaataaca
     3601 tattcaatcc ctttgatcag cgctttattg aacccctgta cgatttcggg cttttcgtta
     3661 acaagctttt cacccgcgac aagaacattt ccaaaagaag gcaggaattg atcacaaggg
     3721 atttcggcgg cgggaaaacc ggcataattc aattcgatgg ttctgagttt tgaaaagaca
     3781 atggcatcta cctgaccttc cgtcaaggcg ttcaaaatgg cgcctgtgcc gacgatttcg
     3841 acttgaacgt catcgattga cattccgacg ctcttcatgg ctgcttccaa ttggaaataa
     3901 ttagcgctgc caagactggt aacggctacc ttcttccctt tcaggtctgc cggggtttta
     3961 ataccggaat tcgaattgaa gataatggca cccagccctt gttgataggt agtgttaaca
     4021 actttaattg gaattccttg cgctctcgct gcgacgacgg catcggcatt cgggaagccg
     4081 aattccacgt ttccggaagc gacatttttt acgatatctg ccgctgcggc gt
//
LOCUS       DAOMIV010000136         4129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22409_length_4129_cov_3.537064, whole genome shotgun sequence.
ACCESSION   DAOMIV010000136 DAOMIV010000000
VERSION     DAOMIV010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22409_length_4129_cov_3.537064"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PKV59_04270"
     CDS             <1..426
                     /locus_tag="PKV59_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46002.1"
                     /translation="QEIDEALLDEKVPRLVMQPIVENAIEHGIAPHRNGDLILRIYRQ
                     EEKIVMEVENSGELTDSDKEAVDRLLNWDGNDTKEIKPGRLGIRNVNLRLKFLYGKEC
                     GLTITSLPNQRTLARIILLNQSNSSVSLYNPDNQTKLYK"
     gene            525..1784
                     /locus_tag="PKV59_04275"
     CDS             525..1784
                     /locus_tag="PKV59_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46003.1"
                     /translation="MKKRSIFIVGLVIIFSLMIASVAFAEEITVVTSYGGDDGNRGNY
                     EAAVKMYEEQTGNKVNDESNTSNEEWKAKIMTDFETGAEPDVLFFFNGVDSNPFVKAG
                     KVVSIDEIREVYPDYASNMKDSMLGASPVDGKNYSVPVNGYWEGLFVNKKVLDEAGVA
                     VPGADYTWEQFLNDCEKIKTAGFTCVAVSLQEVPHYWFEYTVFNHGSVANHLDIPKTA
                     DDAVAQKWAAGLEDIKVLYEKGYLPANTLTATDAETFQLMADHKAAFAIDGSWKIGWF
                     QSNADDINNFTVTYVPAQGDRAATDIIGGLSMGYYITRKAWNDPEKQKACVDFVGMMT
                     SDDVVSTFGATAVTALKKGTTPPQDADALVTAALAMTKGATGISPAVQDLLTGEARAD
                     LFANVKNVVTGKITATDAIASMISFNQ"
     gene            1887..2777
                     /locus_tag="PKV59_04280"
     CDS             1887..2777
                     /locus_tag="PKV59_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP46004.1"
                     /translation="MSSTIYKNKLPLIIFLVPALILMTIFLYYPFIMNIFNSFSDISG
                     LGTAAKGLNDPWFKNYIRLSQDPNMITAVKNTMILMLSTIIFQVGIALLLAIFVDSIK
                     VGSQFFRTVYFFPLVISATALGLLFNLVFLYDGGMLNQILQKFFGFTQNIDWKDENHF
                     MLTMLSPVMWQYVGFYFVIIVTGLSNIPQEIFEAASLDGAGFWKRTYYISLPMIRNVL
                     ITCLILAITGALKVFDLPWVMMGSGIPMDESWLTGTYMYNQTFLRSDVDYGSTIAVVI
                     VILGVVISQIANKIFKEKEY"
     gene            2779..3666
                     /locus_tag="PKV59_04285"
     CDS             2779..3666
                     /locus_tag="PKV59_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP46005.1"
                     /translation="MGNKRFSSKRIHSYSFTTLFSYTVLIIWAITTIYPFVWVLINSF
                     RVKGQIRSDSFSIPWPWLDSFTLDNYSTAFERSNVFRAYGNSLIISIVVTLFVSLFAG
                     FAAYGLVRYSFRGKEILHSLIIASMMFPVFSTIIPVYRMEVHWGIASTGKLPLSLLST
                     ILPQIAGNLSFAIVVLMGFIQSIPIDLEESAFIDGCNVFQIYFRIILPVAKPSFATVA
                     IFTFLWSYNDLFTQMFFLRRPKERTITLLLNEINSQAGVNYGLMAAAVVIVVVPVLIV
                     YVLLQKNIIKGMVAGAIKG"
     gene            3706..>4129
                     /locus_tag="PKV59_04290"
     CDS             3706..>4129
                     /locus_tag="PKV59_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQP46006.1"
                     /translation="MYKVVIVDDEEIIVRGLQSVVNWNSYKCEVVATAFDGISGAIAV
                     RTYLPDILITDIKMPNRDGLTMLAGLRSEFPAMQVTILTGYADFDFAQKAIKLGVSRL
                     LLKPSRMSEINEALQFMTDTLAKRSPVASDDKIDDLSTA"
BASE COUNT         1177 a          755 c          897 g         1300 t
ORIGIN      
        1 caagaaattg atgaggcgtt attggatgaa aaagtacctc gcttggttat gcagccgatc
       61 gtcgaaaatg caatcgaaca cggaatagcc cctcatcgaa acggcgatct aatattaaga
      121 atctatcggc aggaagagaa aatcgttatg gaagttgaaa atagcggaga attgaccgat
      181 tctgacaaag aagctgttga tcgtctttta aattgggatg gaaatgatac aaaggaaatt
      241 aagcccggcc gtcttggtat tcgaaatgta aatttacgct tgaaatttct ttatggaaag
      301 gaatgtggat tgacgattac ctctcttccc aatcagagaa cgctagcaag aatcatttta
      361 ttgaatcaat cgaattcttc cgtaagcctt tataatcccg ataatcagac aaaattatac
      421 aaataaaaac aagttttact atgtaacaaa catcttggtc aatctacaat aaatatacaa
      481 atattcaaac cgttttcata acggtaaaag aacgggagaa agaaatgaag aaaagatcta
      541 tatttatcgt tggattggtg attattttta gtttaatgat tgccagtgtc gcttttgccg
      601 aagagatcac tgtagttacc tcttatggcg gtgatgatgg aaaccgcgga aactatgaag
      661 cagcggtcaa aatgtatgag gaacaaacgg gaaataaagt caatgatgaa tcgaacactt
      721 ctaacgaaga gtggaaggcg aaaatcatga ctgattttga aacaggtgcg gaacccgatg
      781 ttctgttttt cttcaacggt gtggattcca atccgtttgt gaaagccgga aaagtcgttt
      841 caatcgatga aattcgtgag gtttatccgg attatgcttc gaatatgaag gatagcatgt
      901 tgggcgcttc tccggtggat gggaaaaatt attcggtacc ggtaaacgga tattgggaag
      961 ggttgtttgt caacaaaaaa gtactagacg aagccggtgt cgcggtaccg ggagcggatt
     1021 atacctggga acaattcctg aatgattgtg aaaagattaa gactgccgga tttacctgtg
     1081 tcgcggtttc tcttcaggaa gttcctcatt attggtttga gtacaccgta ttcaatcatg
     1141 gttcggttgc aaatcattta gatattccga aaactgccga tgatgccgtc gctcaaaaat
     1201 gggctgccgg attggaagat atcaaagttc tctatgaaaa gggatattta ccggcaaata
     1261 ccttaactgc aactgatgca gagacttttc agttgatggc tgatcataaa gcggcatttg
     1321 ccatcgatgg cagttggaaa ataggctggt tccaatctaa tgcagacgat attaataatt
     1381 ttactgtgac atatgtcccc gctcagggtg atcgtgccgc aaccgatatc atcggcgggc
     1441 tttcgatggg ttattacatt actcggaaag cttggaatga tcccgaaaaa caaaaggctt
     1501 gcgtggattt cgtcggtatg atgacctctg atgatgtcgt ttccactttc ggagcaaccg
     1561 ctgttacggc attaaagaaa ggaacaactc ctcctcaaga tgcagatgct ttggtaacag
     1621 cagcgcttgc gatgacaaaa ggtgcaaccg gaatttcgcc tgccgttcag gatcttttga
     1681 ccggtgaggc tcgggcggat ctttttgcaa atgttaagaa tgttgtcacc ggcaaaataa
     1741 ccgctacgga tgcgattgct tcaatgatct cattcaacca ataaaaaata tcaaaatgtg
     1801 ataaaaaatg agggacagac agggatgcct gtccctcatt gataataaaa ccgaaatcat
     1861 tcaaaaaata ttaaagaaaa aacataatga gttcaacgat ctacaaaaat aaacttcctt
     1921 taattatttt cttagtgccg gcactgattc tgatgaccat ctttttatat tatccgttca
     1981 tcatgaatat attcaacagc ttttcggata tttcaggttt aggaactgcc gctaagggat
     2041 tgaatgatcc ttggtttaag aactatattc gattatctca ggatcccaat atgattaccg
     2101 ccgtaaaaaa cacgatgatc ctgatgcttt cgacaatcat ttttcaggta ggaatcgccc
     2161 tccttcttgc gatttttgtg gattccatca aagtaggatc tcagtttttt cgaacagttt
     2221 atttcttccc tttggtgatc agtgccacgg cattgggatt attgtttaat cttgttttcc
     2281 tttatgacgg tgggatgtta aatcaaatac tccaaaaatt ctttgggttt actcaaaata
     2341 tcgattggaa agatgaaaat cactttatgc tgacgatgct gtctccggtt atgtggcaat
     2401 atgttggatt ttattttgtc atcatcgtca ccgggctcag taatattccg caggaaatat
     2461 ttgaagctgc ttcattggac ggggccggat tttggaaacg gacatattat atttccttac
     2521 cgatgatccg aaatgtcttg attacctgtt tgattttggc aattaccggc gctttgaaag
     2581 tttttgattt accgtgggtg atgatgggtt cgggaattcc gatggatgaa tcctggttga
     2641 cggggactta tatgtataat caaacgttct tgcgttcgga tgtagattat ggttcaacta
     2701 ttgctgtcgt aatcgtcatt ctgggtgtag ttatttctca aatcgcaaat aagattttta
     2761 aggaaaaaga atattagtat gggaaacaag cgattttctt cgaaaagaat tcattcatac
     2821 agttttacga ctttattttc ctataccgtt ctgatcattt gggcaatcac aacgatttat
     2881 ccttttgttt gggtactgat caattctttt cgagtcaaag ggcaaattcg ttcggattct
     2941 ttttccattc cgtggccgtg gttagattct tttactttgg ataattattc caccgctttc
     3001 gaacgctcaa atgttttccg agcgtacgga aacagcctca tcatttcgat tgtcgtaaca
     3061 cttttcgtaa gtctttttgc gggatttgcc gcatatggat tggttcgata ttcatttcga
     3121 gggaaagaaa tccttcattc attaatcatt gcaagcatga tgtttccagt attttctacc
     3181 attatcccgg tttatcgaat ggaagtccat tgggggattg ccagcactgg caaactgcct
     3241 ttgtctttat tgagcacgat tttgccgcag atcgcgggaa atctttcctt tgccatagtc
     3301 gtattgatgg gattcattca atccattccg attgatttgg aagaatccgc atttatcgat
     3361 ggttgtaatg tttttcagat ttattttcga atcattcttc cggttgcaaa accctctttc
     3421 gcgaccgtgg caatttttac ttttctttgg tcctataacg acctttttac tcagatgttc
     3481 tttttacgca gacccaaaga acggacaatt actctccttt taaacgaaat caattctcaa
     3541 gccggagtaa attatggatt gatggccgca gcggtagtga ttgttgtcgt tcccgttttg
     3601 attgtttatg tattgttgca aaagaatatt atcaaaggga tggttgcggg tgccattaaa
     3661 ggttgatttt cgtggtttgt aataagatct cgtataattt ttattatgta taaagttgtc
     3721 atcgtcgatg atgaggaaat tattgttcgc ggactgcaaa gtgttgtaaa ttggaattct
     3781 tacaaatgcg aagtcgtcgc aacagctttt gacggtattt ccggagctat tgcggttcga
     3841 acctatctgc cggatatttt gattacggat atcaaaatgc cgaacagaga cggcttgaca
     3901 atgctggcgg gacttagaag tgaatttcca gccatgcagg taacaattct gaccggttat
     3961 gccgattttg attttgctca aaaagcgatc aagctcggtg tcagccgtct tttattaaaa
     4021 ccgtcacgta tgagtgaaat taacgaagct ttgcaattta tgaccgatac actcgcaaaa
     4081 cgatctcctg tcgcatcgga cgacaaaatc gatgatttaa gcaccgctt
//
LOCUS       DAOMIV010000137         4103 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22592_length_4103_cov_3.348320, whole genome shotgun sequence.
ACCESSION   DAOMIV010000137 DAOMIV010000000
VERSION     DAOMIV010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22592_length_4103_cov_3.348320"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PKV59_04295"
     CDS             complement(<1..900)
                     /locus_tag="PKV59_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP46007.1"
                     /translation="MAIKNTFMKKSARKEARTGYLMIAPLMIGLLIFSVRSFFVNFYY
                     SFTNKSAFGNPKFIGLANYEKLFHSDKFYAALGNTFLYVLLCVPFVIAISLLLAVLLN
                     KGINLTGFYRTLIFIPAVTMPAAIGLIWKWILNYEFGLLNAIIRILGGQPQAWLSDPN
                     YVLLSVSAVLIWADVSLRMIVFLAGLQNIPKSLYEAAEIDGANNRTKFFKITLPMLSP
                     TIFFVTLMEIIGVFQIFDFIYLMIPMNSSGMPAARSLVSYFYEEAFVRNNRGYGSAIT
                     VILFVIIFIITMIQMKLQKKWVFT"
     gene            complement(1141..2415)
                     /locus_tag="PKV59_04300"
     CDS             complement(1141..2415)
                     /locus_tag="PKV59_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQP46008.1"
                     /translation="MLKKVKNVSLILCIVLLLGIFSGANAAAEKTKISFMLWDEVQSP
                     VFETIIGKFMEANPDIEVELQLTPWNQYWTKLDAAAGAGTAPDVFWMNVFMPKYTDAG
                     ILEPLDSYIEKDGLDKSAWVPAMMELYSDKGVQYGMPKGMDVVVVAYNKGIFDKYGVE
                     YPKEGWTWDEFVAKGAELRDKIAEKGGQEYPLAMEIDEPQPSYLQLLVQEGLKIYSDD
                     GLTTDLNDPKAVKPFADIVKLMDDKILPEYKILSDTKATDLFISEKAAMLYVGSWKSS
                     VLDSASFAKNIGLIPMPTRENNHCALGGISYSMSASSKNKEAAWKLISYLSGPESNRI
                     QAEARIEIPAYIAVQDAYGPTFQNIDGSIFIEQSKSAVKYPVHRAIATTTQIISDNAA
                     AIFSRTVTPEEGVAKMAKEVQAAIDEYNATMK"
     gene            complement(2468..3475)
                     /locus_tag="PKV59_04305"
     CDS             complement(2468..3475)
                     /locus_tag="PKV59_04305"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP46009.1"
                     /translation="MDKIDIRFIASQAQVSPATVSRVLNKTKTVSPELRTRVLNTIEK
                     YDYRPNQVARGLINHKTRLIGIVTPKVSNAFHAELISQIEAEADRAHYNVIISNILGN
                     FENEKKTFRIMKDRQVDGIILLHENTPDEMRELEEIVDFPIVLASVRVKDSLLPTVAI
                     DDERAAFEAARYLCQLGHVRIAGVFSDSYSTGTLRRKGFLEGLQSWGIDRNSISLYTT
                     ECSFGGGIAVATEIKNQKNLPTAVFFASDEMAIGAINYFQDHGIRVPEDISVFSFDDI
                     ELSGFVRPRLSTIHQPIQDIGRKATDLLIKLIDEKPLAQREFLLPHQVIVRDSCANPR
                     D"
     gene            3744..>4103
                     /locus_tag="PKV59_04310"
     CDS             3744..>4103
                     /locus_tag="PKV59_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46010.1"
                     /translation="MFFRYPISDVHIHVFRQEDAFDVIKMADELSYDQYNILSATSLG
                     PRFAADNLLTAWLKIKEPYRCHGFAGFNYPEMGAPEAEDLLKQAQDFHALGFDGIKMM
                     DGKPGIRRKIGKPLDDPL"
BASE COUNT         1149 a          894 c          848 g         1212 t
ORIGIN      
        1 cgtaaatacc cattttttct ggagtttcat ttgaatcatg gtaatgataa aaatgataac
       61 gaacagaatg accgtaatag ccgaaccgta tcctctgtta ttgcggacga aggcttcttc
      121 gtaaaaataa cttaccaaac ttctggctgc cggcattccg ctgctgttca tgggaatcat
      181 caaatagata aaatcgaaaa tttgaaagac cccgataatt tccatcaatg tcacgaaaaa
      241 tatcgtggga gaaagcatcg gtaatgtgat tttaaaaaat tttgtccggt tatttgctcc
      301 gtcaatttca gctgcttcgt agagggattt gggtatgttt tgcagcccgg ccagaaaaac
      361 aatcattcgc aaactgacat ccgcccagat taacaccgcc gataccgata aaagaacata
      421 attcggatca gaaagccaag cttgcggttg tcctccgagt attcgaatga tcgcattcaa
      481 taaaccgaat tcataattga gaatccactt ccaaattaaa ccgatggctg cgggcatcgt
      541 aaccgcggga ataaaaataa gcgtccgata gaaaccggtc aggttaatcc ctttattcag
      601 taaaaccgcc aaaagaagag aaatcgcaat cacaaaaggg acgcataaga gcacataaag
      661 gaaggtattc cccagcgcag cataaaactt atccgaatgg aataatttct cataatttgc
      721 gaggccgata aatttcggat tcccgaatgc ggatttattg gtaaaactgt aatagaaatt
      781 gacaaaaaac gaacggaccg aaaaaatcag aaggccgatc atcagcggag cgatcatcag
      841 ataaccggtt cgagcttctt ttcgagctga tttcttcata aaagtatttt ttattgccat
      901 aactgatata ccatcctatg aaaatcaacg gagcggatca aatcattcgg gtttcaaata
      961 ttaatgataa tgcttctcgg tatcagaaaa acacccctaa ataaactgcc ggtccggttg
     1021 attctcttga aataacggac acttcggact ttttgagaag cggtttgata acgatggcag
     1081 caaatatgaa taaatgcgcg gctgccgaaa aatcggcagc cgctgcaatt ctcgaagagt
     1141 ttatttcatg gttgcattgt attcatcaat ggcagcctga acttcttttg ccattttcgc
     1201 tacgccttct tcaggagtca ccgtcctgga gaaaattgcc gcagcattat ccgaaattat
     1261 ttgggttgta gtggcaatcg cccgatgtac cgggtatttg accgctgatt tgctttgttc
     1321 gataaagata ctgccgtcga tattttggaa ggtcggtccg taagcatctt gtaccgcgat
     1381 ataagccgga atttcgattc tggcttccgc ttgaattctg tttgattcgg gtccgctcaa
     1441 ataggaaatt aatttccagg ctgcttcctt attctttgaa gaagcgctca tcgaataact
     1501 gattccgccc aacgcacagt gattattttc gcgggtcggc atcgggatta atccgatatt
     1561 tttggcgaaa gaagcgctgt ccaagacaga agatttccag gatccgacat acaacatggc
     1621 ggctttttcg gatatgaaca agtcggtggc ttttgtatcc gataagattt tgtattccgg
     1681 taaaatctta tcatccatca gtttgacgat atccgcgaac ggttttaccg ctttgggatc
     1741 gttcaaatcg gtcgttaaac cgtcgtcact gtatattttt aatccttcct gaaccaacag
     1801 ttgcaaataa gacggttgcg gttcatcgat ctccattgcg agcggatatt cctgccctcc
     1861 tttttcggca atcttgtcgc gcaattcggc tccctttgct acaaattcat cccaagtcca
     1921 accttctttt ggatattcga cgccgtattt atcaaaaatg cctttattgt atgcgacgac
     1981 aacgacatcc atccctttcg gcattccata ttgaactcct ttgtcgctgt acagttccat
     2041 catggcgggc acccaggctg atttatcgag gccgtctttt tcgatataac tgtccaacgg
     2101 ttccagaatt cccgcatccg tgtatttggg cataaaaacg ttcatccaaa acacatccgg
     2161 agccgtaccg gcgcctgctg cggcatccaa tttcgtccaa tattgattcc acggagtaag
     2221 ctgcagctcg acttcgatat cggggttcgc ttccatgaat ttcccgatta tggtctcaaa
     2281 taccggactt tggacttcat cccatagcat aaatgaaatc ttggtttttt cggcggcagc
     2341 atttgcaccg ctgaaaattc ctaaaagcag gacaatacaa agaattaagc ttacattttt
     2401 aacttttttc aacatttttt cctccttaaa tgtttttatc cttttgataa tcggttaatt
     2461 gactgaatta atctctggga ttcgcgcaac tgtctctgac aattacctga tgcggcaata
     2521 aaaactcccg ttgtgccaaa ggtttctcat cgattaattt gattaaaaga tccgtcgctt
     2581 ttcgaccgat atcctgaatc ggttgatgga tcgttgaaag acgggggcgg acaaaaccgg
     2641 ataactcgat atcatcgaaa ctgaataccg aaatatcttc cggaacgcgg attccatggt
     2701 cttggaaata gttaatcgct ccgattgcca tctcgtcact ggcgaagaaa acggcggtgg
     2761 gaagattttt ttgatttttt atttcggtcg caaccgcgat tcctccgccg aaagaacatt
     2821 cggttgtata caacgaaatt gaattccgat caatccccca actttgaagt ccttctaaaa
     2881 accctttccg gcgaagcgtg ccggtcgagt aagaatcgct gaaaacaccc gctattcgta
     2941 catgaccgag ctgacaaaga taacgggcgg cttcgaaagc cgctctttca tcatcgattg
     3001 caacagtcgg caaaagactg tctttaaccc gaacactcgc cagaacgatc ggaaaatcga
     3061 cgatttcttc taattcacgc atttcatccg gtgtgttttc atgtaataaa ataattccgt
     3121 ccacctgccg atctttcatt atccgaaatg tctttttttc attttcaaaa ttccccaaaa
     3181 tattggaaat aatcacgttg taatgagccc gatcggcttc cgcttcgatt tgggaaatca
     3241 attccgcatg gaatgcattg gaaaccttgg gcgtgacgat gccgatcaat ctcgttttat
     3301 ggttaatcaa accgcgagcg acctgattgg ggcgataatc atatttttcg atcgtattca
     3361 aaacccgtgt tctgagctcg gggctgactg tttttgtttt atttaaaacc cgtgaaaccg
     3421 ttgccggtga aacctgagct tgtgatgcga tgaatcgaat atcgatttta tccatttatc
     3481 ttctctcacc ttttaaaaca agaaatcgta tcggacttta taaaattcat tatataggaa
     3541 aaccttttcc tgtcaagaaa ataaaggaac gaatttaatc acagcttttt tatgagttat
     3601 tgcgcggaat attcaagaat ttagttgttt tttactatac attcttgaat ccttttaggg
     3661 ttgacaggaa acccttttca cgtatgattg agttaattaa agcgaattcg attttgtccg
     3721 ctctgtaaaa agacggaggt tttatgttct ttcgttatcc gatttcggat gttcatatcc
     3781 atgtgtttcg tcaggaggat gcgtttgatg ttatcaaaat ggcagacgaa ttgtcttatg
     3841 accaatataa tattttaagc gcgacctcat tgggccccag atttgccgca gataatctgt
     3901 tgactgcgtg gctgaaaatt aaagaacctt accgctgtca cggatttgcc ggttttaatt
     3961 atcctgagat gggtgctccc gaggcggaag atttgttgaa gcaagcccag gactttcatg
     4021 cattgggttt tgacggaatt aaaatgatgg atggaaaacc ggggattcgg agaaaaatcg
     4081 ggaagccgtt ggatgatccg ctg
//
LOCUS       DAOMIV010000138         4086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22745_length_4086_cov_4.611015, whole genome shotgun sequence.
ACCESSION   DAOMIV010000138 DAOMIV010000000
VERSION     DAOMIV010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22745_length_4086_cov_4.611015"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="PKV59_04315"
     CDS             complement(<1..1266)
                     /locus_tag="PKV59_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQP46011.1"
                     /translation="MKNKSENRSMTEGIIWKQLLFFAIPLFLSSILQQFYGIIDSLFV
                     GNFVGDLALAAVGSTGSLVNLLVGFFLGLSAGAGVVIANSYGAGDAVRMEKAKHTAIL
                     VAAICGVALTLLGWILTPVLLRFMNTPDGVYELAIVYLRLYFLGMIPMLIYNVGAGIM
                     RSIGNSREPMMILLFCGIFHIFADYLFITLWKMGVAGAAYATGISQLLSAVWVMWRLI
                     RSDGPDRLEWQRMKIDMPVLGSIIRVSVPAGFQSSMYSIANLMIQSYINMFGSVAMAG
                     SAAYFKIDNFVFSPVTAMGLAVTTFVSQNVGAGRFDRIQRAIRNALMMAFSFSIFLAV
                     IVILFRYPILGWFTKSPAVIETGILFIFYLIPFYPIHNLTEVLSGTIRGAGNATTPMV
                     ITIFCIGIFRIVWVLIMTHFWFDLRMVIVR"
     gene            complement(1905..2660)
                     /gene="nagB"
                     /locus_tag="PKV59_04320"
     CDS             complement(1905..2660)
                     /gene="nagB"
                     /locus_tag="PKV59_04320"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQP46012.1"
                     /translation="MKIKFIPAKDAVEAGRLGADIFTQTMEENPACVFGLATGSTPLS
                     LYSELVLRYSQGKLDFSLVRTVNLDEYTGLPADHPQSYHQFMYDHLFSKINIHPRNTH
                     LPNGMADDLESACREYEKMIESMGGIDLQLLGIGLNGHIGFNEPSDYFPKYTHVVDLT
                     ESTREANKRFFTDIREVPTQAITMGIGTIMSARKILLLCTGQKKAEILHEAMFGKVRP
                     QVPASILQFHPKVIVIGDEEALSVIRRDHPDFL"
     gene            2825..3421
                     /locus_tag="PKV59_04325"
     CDS             2825..3421
                     /locus_tag="PKV59_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQP46013.1"
                     /translation="MKDLLKSIQERRSIYSIAKKSPISDERIRELVSLAVMYTPSAFN
                     MQSQRAVLLLGNHHEKLWSITKDTLRGIVPPEQFKSTEKKIDGFAAGYGSILFFDDTE
                     TVADFQHKFPLYADNFSVWTEHSNGMLQYIAWTLLEAEGFGASLQHYNPLIDEKVKSA
                     WDIPASWKLIAQMPFGLPTAPADKKEFVPIEKRFKAFE"
     gene            3538..>4086
                     /locus_tag="PKV59_04330"
     CDS             3538..>4086
                     /locus_tag="PKV59_04330"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQP46014.1"
                     /translation="MKTNIEIIHPLPPETYIRHALFDFNGTFSLIREGWQEIMIPMMV
                     DLLMQTPEHEEEAEVTRVVKEYVTQLTGKQTIYLMMQLAEEINKRGGTAESPDVYKDI
                     YQTKLLERIQYRREGLRNGNIQPEEYLIPGSVEILQALKSRGVKCYLVSEMDEKYVLE
                     DADLLQLTPYFDEIHGTLDDYVK"
BASE COUNT         1296 a          891 c          791 g         1108 t
ORIGIN      
        1 tctgacgatc accatccgaa ggtcgaacca gaaatgggtc atgatcaata cccaaacgat
       61 gcgaaatatg ccgatacaaa aaatcgtaat taccatcggt gtggttgcgt tgccggcacc
      121 ccgaatggtc ccgctgagca cttcggtcag gttatgaatc ggataaaacg gaatcagata
      181 aaagataaat aaaattcccg tttcaatcac cgccggactt tttgtaaacc aacctaaaat
      241 cggatagcgg aataagataa cgatgacggc aagaaaaatc gaaaaagaaa aggccatcat
      301 taatgcgtta cggatcgccc gctgaattcg atcgaaccgg cctgcaccga cattttggct
      361 tacaaaagtt gtgaccgcca atcccatcgc cgtcaccggc gaaaacacaa agttatcgat
      421 cttgaaataa gcggctgatc ccgccatggc aaccgatcca aacatgttga tataagattg
      481 aatcatcaaa ttcgcaatcg aatacatgga agattgaaat ccggcgggga cacttacacg
      541 gataatggaa cctaacaccg gcatatcgat cttcattcgc tgccattcaa gccgatccgg
      601 accgtccgat cggatcagac gccacatgac ccaaacagcg gaaagaagtt gagaaatccc
      661 ggttgcatat gcggcaccgg ccactcccat tttccaaagc gtaataaaaa gataatccgc
      721 aaagatatga aatatcccgc aaaacaacaa aatcatcatt ggttcacggg aatttccaat
      781 tgaccgcata attccggctc cgacgttata aatcaacatc ggtatcattc ccaaaaaata
      841 cagccttaag taaacgatcg ccaattcata cactccgtcc ggcgtattca taaagcgtaa
      901 cagaaccggc gtaagaatcc aacccagcaa cgtcaatgcg acaccgcaga ttgcggctac
      961 caatatagcc gtatgttttg ctttctccat cctgacggca tcaccggcac cgtaactgtt
     1021 tgcgatgacg actccggctc ccgccgataa tccgaggaaa aaaccgacca ataggttaac
     1081 caacgatccg gttgaaccga cggcggccaa tgccagatca ccgacaaaat taccgacaaa
     1141 aagggaatcg ataattccat agaattgttg cagaatactg cttagaaata agggaatcgc
     1201 aaaaaaaagg agttgcttcc aaataattcc ttctgtcatt gagcgattct cagatttatt
     1261 cttcattccg caacttttta tctgccctta tctttaatcg atttcttttc tgaaaacagc
     1321 aaaaaaacag gaaacaaatt cctttttgaa ttgtatcagg aaccttcaat tcctcatgat
     1381 tatattcatt ttctttcgat tgataggacc accaaagcta agagaataaa aaacatcctc
     1441 cgattgattc atcgaaagat gtttgatttt ttccgcaccg tgcccggttt atctcccctt
     1501 tgggagacaa accttttcac ttttttcctc agttttcaaa atgataatta ctgagaaaac
     1561 aggcaaaaat tgccacagca ataacagaaa tcgtagttaa catgatttcc ttcctttccc
     1621 caataaatgg gcaataaaca accgaaaagg agctgctttc gttgaaaagc cgattgtttc
     1681 tttcaaagga agagactgat ttcgttcctt tgtttctcct attcggtttt caatgtataa
     1741 aagcgatatg acttttgtca tattattaat attacatttg tcacatcgct ttctatatta
     1801 cgcttaattt catcgattgt caaatgaaat gtcataaaaa aatcagacaa gccgtcaaat
     1861 cgagcctgtc tgatatttat gaaaacaatt tttttacaaa tttcttacaa aaaatccgga
     1921 tgatcccggc ggatgacact cagtgcttct tcatcgccaa tgacaattac cttgggatga
     1981 aattgtaaaa tggaagccgg aacctgaggc ctgacctttc caaacatcgc ttcatgcaga
     2041 atctccgctt tcttttgccc cgtgcataac aataaaattt ttcttgcgct cataattgtt
     2101 ccgattccca tggttatggc ttgggtcggg acttccctga tatcggtaaa aaagcgtttg
     2161 tttgcttcac gggtactttc cgtcaaatcg actacatggg tatattttgg aaaataatcg
     2221 gaaggttcgt tgaaaccaat atgaccgttg aggccgattc cgagtaattg caaatcgata
     2281 ccccccatac tttcgatcat tttttcatat tcccggcagg cactctccaa atcatcggcc
     2341 ataccattcg gaagatgtgt atttctggga tgaatgttaa ttttcgaaaa aagatgatca
     2401 tacataaatt gatgataact ttggggatga tcagcaggta agccggtgta ctcatccaaa
     2461 ttcaccgtcc gaactaatga aaaatctaac tttccctggc tatatctcaa aacaagttca
     2521 ctgtataacg aaagcggagt cgacccggtc gccaatccga acacacaagc gggattttcc
     2581 tccattgttt gagtaaagat gtctgccccc aagcgaccgg cttctaccgc atctttcgca
     2641 gggataaatt ttattttcat gacaaatcct ccaaaaattt tgacctttaa taagaattga
     2701 ttgattatca ttcctaaaag ggattaggat aaagataaac aattgaccaa atcataacgc
     2761 aagaaagaat tattgttaaa tcgtcaattg gaatctataa atttttgatt cggaaaggat
     2821 ctttatgaaa gacttgctca aaagtattca ggaacgcaga agtatttatt ccatcgctaa
     2881 aaaatcaccg atttcggatg aaagaatccg ggaattggtt tctctggctg tcatgtatac
     2941 tccttccgct tttaatatgc agagtcagcg agcggttttg ttgctcggga atcaccatga
     3001 aaaattatgg tcgataacaa aggatacttt acgcggtatc gttcctccgg aacaatttaa
     3061 aagcaccgaa aaaaagatcg atggttttgc tgccggatat gggtcaattc ttttttttga
     3121 cgataccgaa acggtagccg attttcaaca taaatttccg ctttatgcgg acaatttttc
     3181 cgtttggacc gaacactcca acggcatgct gcaatatatt gcctggacgc tgttggaagc
     3241 ggaaggattc ggtgcctctt tgcaacatta caacccgttg attgatgaaa aagtaaaatc
     3301 cgcgtgggac attccggcat cttggaaact cattgcgcaa atgccgtttg gattgccgac
     3361 ggctccggcc gataaaaaag aatttgttcc gattgaaaaa agatttaaag catttgagta
     3421 aataaaattt attttgatat cataatacta atttctcata aaataacgga gagaatcaaa
     3481 ataaaagtcg gaagcgatta gaattaaagc caaaaattag aaggaaatcc gttttagatg
     3541 aaaacgaata ttgaaatcat tcatccgctg cctcccgaaa catacatacg ccatgctctg
     3601 tttgacttta atggtacttt ttcactgatt cgtgaaggtt ggcaggaaat tatgattccg
     3661 atgatggttg atttactgat gcagacgccg gaacatgaag aggaagccga agtcacgcgg
     3721 gtggtaaagg aatatgtcac tcaattaacc ggaaaacaaa ccatttacct gatgatgcag
     3781 ctggccgagg aaatcaacaa acgtggcgga acagccgagt ctcccgatgt ttataaggat
     3841 atttatcaaa ccaaattact ggaacggatt cagtatcgcc gtgaagggct gcggaacggc
     3901 aatatccagc ccgaggaata tttgatcccc ggatccgtag aaattcttca agcccttaaa
     3961 tcccgaggtg tcaaatgtta tctggtatcc gaaatggatg aaaaatatgt attggaagac
     4021 gctgaccttc ttcaattaac cccttatttt gatgaaatcc atggcacgtt ggatgattat
     4081 gttaaa
//
LOCUS       DAOMIV010000139         4083 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22764_length_4083_cov_4.113208, whole genome shotgun sequence.
ACCESSION   DAOMIV010000139 DAOMIV010000000
VERSION     DAOMIV010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22764_length_4083_cov_4.113208"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PKV59_04335"
     CDS             <1..1129
                     /locus_tag="PKV59_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQP46015.1"
                     /translation="PVFMLDEIDKLGNDYRGDPSAALLEVLDPEQNSRFSDHYLEVDY
                     DLSKVMFITTANSIEMIPDALLDRMELIEFPGYIEEEKLIIAKDYLINRQTEENGLEP
                     QDINFTDDAIKRIIREYTYEAGVRNLEREIGKICRKIARLKAEGKEYEREISAESVGK
                     YMGPPYFFQSEKEKEDEVGVATGMAWTPNGGDITTIEVLVFDGKGNLQVTGQIGDVMQ
                     ESAQAALSFVKSRAKQFNIPKDYFEKTDVHIHIPEGAVPKDGPSAGVTLAIALISAIT
                     NTPVKKDIAMTGEVTLRGNVLSVGGIKEKVLAAHRLGIKTIIIPERNEKDLEELPQQI
                     KEELTITSVNHMDQIIPLAFGNIQPKRKTEVRAKKDKKDES"
     gene            complement(1215..2573)
                     /locus_tag="PKV59_04340"
     CDS             complement(1215..2573)
                     /locus_tag="PKV59_04340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQP46016.1"
                     /translation="MPNQEEKKENTHPLLLNKERKSRHEKEMKQMKWLKIGGITVFAI
                     VILVLVFGAFYEYQYLPNKTIAKVQDKKITVKEFQEAVRYQRVNLISNYNYMAQLYQS
                     FGMQMDEETKSQYETQLSEPYAGILGQQVYNTMVNQLILEYGAKAAGITVSDAEVDNE
                     IQSMWSYYPNGTPTPRPSETPFANTPTVSEEQLKLLNYTETPTPTTEISDLTSLKESG
                     PEANTPIVTVTPTLSPTATQTEVSSESPTPVLSPTPSLSPTTYTEEMFQNNFNRQFED
                     NPYYSKEFFRKQVYYQLLEQKVRDMLAKDIEPEAEMVWARHILVSTEDEAKKVIERLN
                     NGEDWNALAAEVSIDTSNNTKGGDLGWFMKGVMDPDFEKAAFEQEVGTYSKEPVKTQY
                     GYHVIQVVGHEVRPLTSTDRSNAIDRAYKNWLENAKSNLKIETGSDWVDFVPVEPTLS
                     DL"
     gene            complement(2649..2906)
                     /gene="rpsR"
                     /locus_tag="PKV59_04345"
     CDS             complement(2649..2906)
                     /gene="rpsR"
                     /locus_tag="PKV59_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQP46017.1"
                     /translation="MDHRGNRRFIARPKFCQFCSEKNTKIDYKNADLLRRYITEEGKI
                     RPRRQTGTCAKHQRQIAAAVKRARYIALLPYTSEPYQDVVR"
     gene            complement(2979..3266)
                     /gene="rpsF"
                     /locus_tag="PKV59_04350"
     CDS             complement(2979..3266)
                     /gene="rpsF"
                     /locus_tag="PKV59_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQP46018.1"
                     /translation="MRKYEIVIIANPDLDEESINGIVEKVSGWIKDSGGEIEKVDDWG
                     KRRLAYRIQKQREGHYVLITANIEPSAMKEISNNLRFVESVLRSMITLVEE"
     gene            complement(3429..3803)
                     /locus_tag="PKV59_04355"
     CDS             complement(3429..3803)
                     /locus_tag="PKV59_04355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQP46019.1"
                     /translation="MSSKFIRNRNVSFRNAFNGIIHGFVDEINFKIHLFFTILAVLLS
                     VILKISLTEWCLIFIVIGMVWSTELINTAIERAVDLYTLEKHPLAKQSKDIAAGAVLI
                     SAITSVMIGVIIFLPKILIQFF"
     gene            complement(3793..>4083)
                     /gene="ybeY"
                     /locus_tag="PKV59_04360"
     CDS             complement(3793..>4083)
                     /gene="ybeY"
                     /locus_tag="PKV59_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQP46020.1"
                     /translation="TDVLTFQSEGEIDPETNIPYLGDILICIERASEQAEISGHSLEN
                     EVELLLIHGLLHLIGFDHDTPANKDLMWEKQTDYLNKFNIHLGRKPGDDFEF"
BASE COUNT         1171 a          824 c          829 g         1259 t
ORIGIN      
        1 tccagtgttt atgttggatg agattgacaa actcggcaat gattatcgcg gagatccgtc
       61 tgccgcattg cttgaagtac tggatccgga gcaaaacagt cgcttctcgg accattattt
      121 ggaagtggat tatgatctct cgaaagtaat gtttattacg actgcaaatt cgattgaaat
      181 gattccggat gcgttgttgg atcggatgga gttgattgaa ttccccggat atatcgaaga
      241 agaaaaatta atcattgcca aagattattt gattaaccgt cagacggaag aaaatggatt
      301 agagcctcag gatatcaatt tcacagatga tgccattaag agaatcattc gggaatatac
      361 ctatgaggca ggtgtcagaa accttgaacg ggaaatcggc aaaatatgcc ggaaaatagc
      421 cagattaaag gctgaaggaa aagaatacga gcgagaaata tcggctgaat ccgtcggaaa
      481 atatatggga cctccttact ttttccaatc cgaaaaggaa aaggaagatg aggtcggagt
      541 cgcgaccgga atggcgtgga caccgaacgg cggcgatatt accacgattg aagtattggt
      601 ttttgacgga aaagggaatt tgcaggttac gggacaaatc ggtgatgtga tgcaggaatc
      661 agcccaagct gctttatcat ttgtgaaatc gcgggcgaaa caatttaata ttcccaaaga
      721 ctattttgag aaaacggatg tgcatatcca tattccggaa ggagcggttc cgaaagacgg
      781 tccgagtgcg ggagtgactt tagctatcgc tttgatttcc gccattacca atacgcccgt
      841 aaaaaaagat attgcgatga ccggtgaagt aacacttcgc gggaatgttt tgtcggttgg
      901 cggcataaaa gaaaaggtgt tagccgctca ccgattagga atcaaaacga ttattattcc
      961 cgaaagaaac gagaaggatc tcgaggaact tcctcaacag atcaaagaag aattgacaat
     1021 tacttcggtc aaccacatgg atcaaattat tcccttggct tttgggaata ttcaaccgaa
     1081 aaggaagact gaagtgcggg ccaagaaaga taaaaaggac gaatcctaag cattatctgt
     1141 ttttttacga ttgatatcaa gaaacaaaaa cacccgaaaa attgggcgtt tttgtttctg
     1201 gtgatatgaa aagattataa atcagataac gtcggctcta ccggaacaaa gtcaacccaa
     1261 tccgatccgg tttcaatttt caaattgctt ttcgcatttt ccaaccaatt tttataagct
     1321 ctgtcgattg cattcgatcg atcggtgctg gttaacggtc tcacttcgtg accgaccact
     1381 tgaataacgt gatatccata ttgtgtctta accggttcct tgctatatgt gccaacttct
     1441 tgttcgaaag cagccttttc aaaatccgga tccatgacac ctttcataaa ccagcccaaa
     1501 tctccaccct tggtattatt cgaagtgtct atagagactt ccgccgccaa tgcattccaa
     1561 tcttcgccgt tattcaaccg ctcgataact tttttggctt catcttccgt ggaaactaag
     1621 atatgtcttg cccaaaccat ctccgcttcg ggctcaatat cttttgcgag catatctctt
     1681 actttttgtt cgagaagctg atagtaaacc tgctttcgga aaaattcctt ggaataatag
     1741 gggttatctt caaactgcct attgaaatta ttttggaaca tctcttctgt atacgtggtt
     1801 ggggataagc ttggggttgg actcaaaacg ggagtcgggc tttcactgct gacctctgtt
     1861 tgagttgcgg tagggcttaa agtcggagta accgtcacaa tcggtgtgtt cgcctcggga
     1921 ccgctttctt tcaaggaagt aaggtcggat atttcagttg tcggagtcgg ggtttccgtg
     1981 taatttaata gcttcagttg ctcttcggat acggtcggtg tattcgcaaa gggtgtttcg
     2041 gaaggtcttg gagtgggagt tccgttggga taataactcc acatggactg aatctcattg
     2101 tccacttcgg catcacttac ggtaattccg gctgcttttg ccccgtattc caagattaat
     2161 tgattaacca tcgtattata aacttgttgg ccaagaatac ctgcatatgg ttcggatagc
     2221 tgagtctcat attgagattt tgtctcttca tccatttgca tcccaaaaga ttggtaaagt
     2281 tgagccatgt aattataatt gctgatcaaa ttcactcttt gataacggac agcttcttga
     2341 aattccttta cggttatttt tttgtcttga acttttgcga tcgtcttatt cggtagatat
     2401 tgatattcat aaaatgcacc gaaaaccaat accaaaatga caatggcaaa gacggtaatt
     2461 ccgcctattt ttaaccattt catttgcttc atttcttttt cgtgtcgcga ttttctttct
     2521 ttgttcaaga gcaaaggatg cgtattttct tttttctctt cttgattcgg cataatttat
     2581 taactcctta gcaaaatagg gcacagagaa cttccctatg ccctattctt tctttggcga
     2641 atgaatcctt aacgaacaac gtcttggtaa ggttcggaag tgtaaggcag caatgcgata
     2701 taacgtgctc tctttaccgc agcagcgatt tgtcgttgat gctttgcaca ggttcctgtt
     2761 tgacggcggg gacgaatttt accttcttcg gtaatatagc gccgcagcag atctgcattt
     2821 ttataatcta ttttggtatt tttttctgag caaaactgac agaattttgg tctggcaata
     2881 aaccttcgat ttcctctgtg atccatttgc tccgtattta tttcttcact cataattact
     2941 cctgcaaccg gtcgagatca ccggtaaaga taaaaatttt attcttcgac taatgtgatc
     3001 atcgatcgaa gaaccgattc aacaaatcgc aaattatttg aaatttcttt cattgctgaa
     3061 ggttcaatat tcgcggttat cagaacataa tgtccttcac gctgtttctg aatacgataa
     3121 gcgaggcggc gttttcccca gtcatccact ttttctattt caccgccgct gtcttttatc
     3181 cagccactga ctttttcgac aattccgttt atggattctt catcgagatc aggattggca
     3241 atgattacga tttcgtattt tcgcattgct aattcctcct ttccttggga ttgttaccga
     3301 tatcgataat cggtaatgga aagattgcct ttcggcaaat caaacgccca aattgtaaca
     3361 tatttttaca aaatagtgac tgccttgctc gatttgtgaa tcaaaatgat gagtcaataa
     3421 aacgaaattt aaaaaaattg tatgagaatc ttcggaagga aaattattac tccgatcata
     3481 acacttgtaa tcgcagatat taatacagct ccggctgcga tgtctttgct ttgttttgca
     3541 agaggatgtt tttccaatgt gtataaatct accgcccgtt cgattgccgt attaatcaat
     3601 tccgtcgacc aaaccattcc gatgacaatg aaaatcaggc accattcagt caaagaaatc
     3661 tttagaataa cgcttaataa aacagctaaa atcgtaaaaa agagatgaat tttaaaatta
     3721 atctcatcga cgaaaccatg aataattccg ttaaatgcat ttcgaaaaga aacattccta
     3781 tttcgaataa acttagaact caaaatcatc tcccggtttt cttccaaggt gaatgttaaa
     3841 cttattcaga taatctgttt gcttttccca cataagatct ttattcgcag gtgtatcatg
     3901 gtcaaatccg attaaatgaa gcaacccgtg gatcagtaat aattcgactt cattttcaag
     3961 agaatgaccg gatatttcag cctgttcgga agctctctcg atacaaatca aaatatcccc
     4021 caaataaggt atgttcgtct cgggatcgat ctccccttcc gattgaaatg ttaaaacatc
     4081 ggt
//
LOCUS       DAOMIV010000140         4080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22785_length_4080_cov_4.048696, whole genome shotgun sequence.
ACCESSION   DAOMIV010000140 DAOMIV010000000
VERSION     DAOMIV010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22785_length_4080_cov_4.048696"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..804)
                     /locus_tag="PKV59_04365"
     CDS             complement(28..804)
                     /locus_tag="PKV59_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46021.1"
                     /translation="MENRIEKDLELINTYLDHEMNDQDLLNFAERLSQEENLKQDLAE
                     IIQVRNQLRKLPMKRPPQNYILTRSMAQEVRKPNLLERFFPIFRTAAVFACIAFVLTF
                     ILPLSSISSSTKTEPMLAKKSVEIDLIDKSDEANSEKVVTPEVFSEESVNPLADSELM
                     VQAFPSKGVRGGNPKLEYLTNAERMIPDDRSPVSTPEISSQPISGEAAQKEIQNLING
                     VSEEETTISAETIIRLVSIGILAVSLFWIAITLIKRRQWA"
     gene            complement(809..1426)
                     /locus_tag="PKV59_04370"
     CDS             complement(809..1426)
                     /locus_tag="PKV59_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HQP46022.1"
                     /translation="METDESKLIKGAIQGNLNAFNQLVMIYQTAVYNTALRIVYDEDR
                     ANDLAQTAFISAWQHMDSFKGDSFKPWILRITINACYDELRRQRRHPSVPLEPVSTED
                     DEENEDVHWLMDPSPQPSQTTETNEMIQAVENCLNRLPFDYRSVLLLIDVQELDYQEA
                     SEVLDKPLGTVKSRLFRARQKMRDCLKEKGELFSSEDRYFDRKDE"
     gene            complement(1473..>4080)
                     /gene="polA"
                     /locus_tag="PKV59_04375"
     CDS             complement(1473..>4080)
                     /gene="polA"
                     /locus_tag="PKV59_04375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQP46023.1"
                     /translation="HIAIAFDTGKTFRNDLYAGYKATRAKMPDDLRPQIDRIRELTDI
                     FGFPRLEMEGYEADDVLGSTAQIAVKDGYDVKIVTGDKDLLQLVTDKILVELSGNKLS
                     ETKEYDVHAVKEYLGILPEQVVDYKALVGDPSDNYPGVPGIGPKTAVTLLNQYGTLDS
                     IYEHLSEIKESIAQKLKANKESAYLSRELAKIRTDLSLDINLALGATDRIDFPAVEKF
                     FKELEFKSLIPKLRTLAPTFQPIQDQLSLFGLSNADDEQPESAHEARCHIIDSEQDLI
                     RMIEKISQANSLSLDTETTGTDPMDCHLVGISLSVETGMGYYIPVGHLTGEPQLDLEK
                     VRQSLNPLFLREDIVKVGHNIKYDLIVLRNHGFEVKGKLFDTMVAGWVIDPESKNLGL
                     KAMAFDLLGINMQSIDTLIGKGSNQLTMANVSIKDAAPYAAADADMTLRLVPILKETL
                     KKRETEKIFDELEMPLIPVLTDMEMAGIRVDRDFLKEMSKSLEIRLSEIEKDIYAGVG
                     YEFNINSTQQLSRAIFETLKLEPPGRKKKTTSGLYSTSADVLEEMRGSHPIIDLILEH
                     RELSKLKNTYVDALPLTINRRTGRVHTSFNQTGAVTGRLASSSPNLQNIPTRTELGRK
                     VRTAFIADPDHVLLSVDYSQIELRIVAHMAQDEGMLAAFRAGQDIHATTAAAIYGCDI
                     SQVTKEMRRHAKAINFGLIYGMSPYGLSQSANLSRKEAEEFVDAYFKKFPRIKEYLDS
                     IKIQAAEKGYVETMMGRKRYFPNLGQQKNYSLRTREEREAINAPIQGTAADIMKLAMI
                     QVHDKLKKSVLQAQILLQVHDELLLECSQKDLDETRELVQDTMENVVTLSIPLLTEAR
                     SGLNWGDLTPMP"
BASE COUNT         1074 a          941 c          832 g         1233 t
ORIGIN      
        1 aaacaagtat cggcgaaacg gattatttta tgcccactgc cttcttttta tcagtgtaat
       61 agcaatccaa aacaggctga cagccaaaat accgatcgaa accaaacgaa tgattgtttc
      121 tgcggatata gtcgtttctt cttcggatac accgtttatt aaattttgaa tttctttttg
      181 tgccgcttct ccggaaatcg gctgtgagct tatttccggt gtcgataccg gtgaccgatc
      241 atccgggatc atccgttccg cattggtcaa gtattccaac ttcggatttc cgccccttac
      301 ccctttcgaa ggaaatgctt gaaccattaa ctcggaatcg gctaacggat taacggattc
      361 ttcgctgaat acttcgggtg taacgacttt ttccgaattc gcttcatcac ttttatcaat
      421 aagatcgatt tcgactgatt ttttcgcaag catcggttcg gtttttgtcg atgaggagat
      481 cgaagacaat ggaagaataa aagtcaatac gaatgcgata caagcaaaaa cagctgccgt
      541 tcgaaatatc gggaagaaac gttcgagtaa atttggcttt cgaacttcct gtgccatact
      601 gcgtgtcaaa atatagtttt gcgggggcct tttcatcggc aatttccgca gctggttacg
      661 tacctggatg atttctgcca aatcttgctt cagattctcc tcttgcgaaa gtcgttcggc
      721 aaaattcaat aaatcttgat cattcatttc atgatcaaga tatgtattga tcagttccag
      781 atctttttct atccgatttt ccattgtcct actcatcttt cctatcaaaa taacgatctt
      841 cggaagagaa aagttcacct ttttctttaa gacaatccct catcttttga cgggcgcgga
      901 ataggcggct tttaaccgtg cccaacggtt tgtccaatac ttcggacgct tcctgataat
      961 ccaattcctg aacatcgatt aataacaaca cggagcgata atcaaaaggt aagcggttca
     1021 aacagttttc aacagcttga atcatttcat ttgtctccgt tgtctgagaa ggctgaggag
     1081 aaggatccat caaccaatga acatcttcat tttcttcgtc atcttcggtc gaaaccggtt
     1141 ccaaagggac agacggatgc cggcgttggc ggcggagctc atcataacaa gcattgattg
     1201 tgattcgtaa aatccatggc ttaaaagaat cacctttaaa agagtccata tgttgccagg
     1261 ctgagataaa agcggtttgt gccaggtcat tggcacggtc ttcatcataa acgatacgga
     1321 gagcggtgtt ataaacagct gtctgataga tcatcactaa ctgattaaaa gcattcaaat
     1381 ttccttgaat ggctccttta atcagtttcg attcgtccgt ttccattttt tctttcatct
     1441 gtaaagcgca tagaacacct tgcgtttatc gatcagggca tcggtgtcag atccccccaa
     1501 tttaagccgc tgcgcgcttc agtcaataac gggatggaca gtgtcacaac attttccatt
     1561 gtgtcttgaa ccaattctcg cgtttcatcc aagtcctttt gagaacattc aagcaataac
     1621 tcgtcatgaa cttgcagcaa aatttgggct tgcaagacgg attttttcag tttgtcatgc
     1681 acttgaatca tggctaattt cataatatcg gcagcggttc cctgaatcgg tgcattgatg
     1741 gcttcccgct cttcacgagt gcgtaaactg taattttttt gctgtccgag attggggaag
     1801 tatcgttttc ttcccatcat ggtttcaaca taacctttct cagcggcttg gattttaatg
     1861 gaatccaaat attctttaat tcgggggaat tttttaaagt aagcatctac aaactcttcc
     1921 gcctctttcc gggaaaggtt cgccgattga cttaagccat agggactcat gccatagatc
     1981 agtccgaaat taatggcttt ggcatgtcga cgcatttctt tggtaacctg ggaaatatcg
     2041 caaccgtaaa ttgctgcggc agtcgtcgca tgaatgtctt gtccggctcg aaatgccgcc
     2101 aacattcctt catcctgagc catatgagcc acaatccgta attcgatctg gctgtaatcc
     2161 accgaaagta aaacatgatc gggatcggcg atgaaggctg tccgaacttt cctgcccaat
     2221 tcggtacgag tcggaatatt ctgcaaatta ggtgaagaag atgccagacg tccggtcact
     2281 gcgccggttt gattgaaaga agtatgaacc cgtcctgttc tgcgattgat tgtgagagga
     2341 agcgcatcaa cataggtgtt ctttaattta gaaagctcac gatgttccaa aatcaaatca
     2401 atgatgggat gagagccccg catctcttct aaaacatcgg cggaagtgga atataaccct
     2461 gaagtagttt tctttttgcg accgggcggt tccaatttga gagtttcaaa aatggctctt
     2521 gacagctgtt gagttgaatt aatgttgaat tcgtagccga cgccggcata aatatctttt
     2581 tcgatttccg aaagccgaat ttcgagtgat ttggacattt cctttaaaaa gtcacgatcc
     2641 acccgaatac cggccatttc catatccgtc aataccggaa tcagcggcat ctccaattca
     2701 tcaaaaatct tttccgtttc tcgttttttt aaggtctctt tcaagatcgg taccaaccgc
     2761 agagtcatat cggcatcagc cgcggcatag ggagccgcat ctttaatcga tacgtttgcc
     2821 attgtcagct gattgctgcc ctttccgatc aacgtgtcga tcgattgcat attgatcccg
     2881 agcaaatcaa atgccatggc ttttaagccg agattttttg attcgggatc aatcacccat
     2941 ccggctacca tggtatcaaa cagctttccc ttgacttcga agccatgatt tcgtaaaaca
     3001 atcagatcat atttaatgtt atgaccgact ttgacaatgt cttcccgcag gaacaatgga
     3061 ttcaaagatt gtcgaacctt ttccaagtcg agttgcggtt ctcccgtgag atgtcccacc
     3121 ggaatgtaat atcccatccc tgtttcgacg gaaagcgaaa tccccaccaa atggcaatcc
     3181 atcggatcgg tccccgtcgt ttcagtatcc aaactcaaag aatttgcctg tgagattttt
     3241 tcaatcattc ggatcaaatc ctgctccgaa tcgatgatat ggcaacgggc ttcatgggca
     3301 gattcgggtt gttcatcgtc tgcattcgaa agtccgaaca aggataattg gtcttgtatc
     3361 ggttgaaaag tgggagcaag cgttcttaat ttcggaatca gcgatttgaa ttcgagttct
     3421 ttaaagaact tttcaaccgc aggaaaatca atccgatccg ttgcccccaa tgccagatta
     3481 atatccaatg ataaatccgt ccggattttt gccaattctc ggcttaaata ggcactttct
     3541 ttatttgctt tcagcttttg agcgatcgat tctttgattt cggataaatg ttcataaatc
     3601 gaatccaatg ttccgtattg attcagcaaa gttaccgctg tttttggacc gatcccgggt
     3661 acgcccggat aattgtccga cggatcgccg acaagggctt tatagtcgac aacttgttcc
     3721 ggcaaaattc ccaaatattc ttttaccgca tgcacgtcat attcttttgt ttcagaaagt
     3781 ttatttccgg ataattccac cagaatttta tccgtcacca attgcagtaa atctttatca
     3841 ccggtgacaa tttttacatc gtagccgtct ttgaccgcga tctgtgccgt actgccaagc
     3901 acatcatcgg cttcgtaacc ttccatttcg agccgaggaa acccaaaaat atccgttaat
     3961 tcccgaatac ggtcaatttg cggacgcaaa tcatccggca tttttgcccg ggtcgcttta
     4021 tatcccgcat ataaatcgtt tcggaaagtt tttcccgtat caaaggcaat ggcaatatga
//
LOCUS       DAOMIV010000141         4078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22795_length_4078_cov_4.609744, whole genome shotgun sequence.
ACCESSION   DAOMIV010000141 DAOMIV010000000
VERSION     DAOMIV010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22795_length_4078_cov_4.609744"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PKV59_04380"
     CDS             <1..990
                     /locus_tag="PKV59_04380"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQP46024.1"
                     /translation="LLCRFFPALEAIPGWKMTILILGLITILISSLMAIFATDLKRVL
                     AYSTVSQLGYMFAAIGAGSVLACQFHLLSHAIFKALLFLCAGAIIHSCGTRDMYKMGG
                     FFKKIPFISVTFIIGSLALAGIPVLNGFWSKELILESIHSGTPIIIYILMLFSVILTA
                     LYTIRCVWLVFFGEPRSDLHVHDAPFLMALPLGILSLGTITSWLVFEPFSRSLSNSLP
                     LYGIPKETLSELVSKIFGDPYTFLALGMVAIGILIWFLRNKLPLHNSITRKIKSISLN
                     SFGFEAINNGVVNGINAASEAIRNIQTGSLNWNIFGMLIGLICILIALMIGGL"
     gene            998..2500
                     /locus_tag="PKV59_04385"
     CDS             998..2500
                     /locus_tag="PKV59_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQP46025.1"
                     /translation="MNPLFWLLITPIICALILYVVGRTIPPDDESSPNKAAWLSLFSI
                     LLTVVPFVLTAKKFTEEGPVSLTAGMITLTFDGISLLMTSVLLVLSAMVVLFSAQYMV
                     AEDHEEKFYGQIMILMSSIMGLTTATDIFNLYVWFELMAVSTYTLVSFYRNRTESLES
                     GIKYLVQSAAGSVLVILGTSILFGLTGEVKISAIRESILTSSPAVIAATACLLIGYGV
                     KCAIVPMHFWLPDAHSQAPAGISALLSGIVIETGLIALLRALSAVSHAHLPLGTIFLV
                     IGCINMFIGTFSALRQKEIKRMLAFSSVSQMGYILLGIGFAISFPASAQNAEGIFTHI
                     FNHALMKGLAFLSAGALVYCIKVSKGNHEPLLLDDLNGVSAKYPLYSLAFTISMLALG
                     GLPLFAGFISKWQILASGIAAQKSWVAFLIALAGINSVLSLGHYAPPINRIYRHVSNN
                     NEVAISPKFQQAMIIPIIILTAAVILLGFFPGITAGITKVASETLYTMFY"
     gene            2513..2827
                     /locus_tag="PKV59_04390"
     CDS             2513..2827
                     /locus_tag="PKV59_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46026.1"
                     /translation="MDILLSPPIGFLIYAIIGTLLVFYGKKTAPVEDTKDDQKRTLYG
                     SGEVAPETRGVPGYKPFLLISLFFALLHLGVLIMGSSNLSVTSLVYTVVLMLGLLALM
                     LG"
     gene            2870..3454
                     /locus_tag="PKV59_04395"
     CDS             2870..3454
                     /locus_tag="PKV59_04395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQP46027.1"
                     /translation="MNKTIDLSDIDPNAKGSEKTIENRIRNWARINSPWAIHYNSGSC
                     NGCDIELLTLITPRYDVERFGVKLQGSPRHADILIVTGSVTLQSKDRLIRIYNQMPEP
                     KFVLGLGSCAISGGVFQNCYSVVQGGVSNVIPVDVFVPGCPPRPEAMINGLIILLDIA
                     RGKRQKSDYSQPMIIPSPEQDSAQTADKKESEAE"
     gene            3454..3945
                     /locus_tag="PKV59_04400"
     CDS             3454..3945
                     /locus_tag="PKV59_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQP46028.1"
                     /translation="MEKEELLNQFQEIFTDLAIKTEIPAIQPNRVDVYINSNDLLKAV
                     QKLVEHDWGYLITISAYDTADENGTPQIGLVYQFGEQNVIGTLRFFLPYSNLTVESIC
                     PIIPSATLYERECMELFGIDIRNTPDRSKLILPENWPDGVFPLRKSFSGLQDNPETKS
                     GEE"
BASE COUNT         1099 a          808 c          891 g         1280 t
ORIGIN      
        1 ctcctttgcc gtttctttcc cgctctggaa gcaatccccg gttggaaaat gacaatccta
       61 attctcggtt tgatcacgat cttgatttca tctctgatgg caatctttgc aacagattta
      121 aagcgggtcc tcgcttattc gactgtaagc caactgggtt atatgttcgc cgcaatcgga
      181 gccggatcgg ttcttgcctg ccaatttcat ctgctttctc atgcaatttt caaggcatta
      241 ctttttcttt gtgccggcgc tattatccat tcctgcggga cacgagatat gtataaaatg
      301 ggcggttttt ttaagaaaat tccgtttatc agcgtcactt ttattatcgg atcattagct
      361 cttgccggaa ttccggtctt aaacggattt tggtcaaagg aattaatcct cgaatccatt
      421 cattccggga caccgattat tatttatata ttaatgttgt tctctgtaat tcttaccgct
      481 ttatatacga tccgttgtgt atggttggtg ttcttcggag aaccgagatc cgatttgcat
      541 gtacatgacg caccttttct catggcttta ccactcggta ttttgtcatt gggaacgatt
      601 acatcttggc ttgttttcga accgttcagc cgatcccttt caaacagttt acctctctac
      661 ggtattccga aagaaacttt gtcggaattg gtcagtaaaa tctttgggga tccgtataca
      721 tttttagctt taggaatggt ggcgatcgga attctgattt ggtttttgag aaataaatta
      781 ccgttacata atagcattac tcggaagata aagtcgattt cgttgaacag ttttgggttt
      841 gaagcaataa ataacggtgt tgtaaacgga ataaatgcag ccagtgaagc gatccgaaat
      901 attcagaccg gctcattaaa ttggaatatt ttcgggatgc tgatcggatt gatttgtatt
      961 cttattgcac ttatgatcgg agggctgtaa catgccgatg aatccgcttt tttggctttt
     1021 gataactcca ataatttgcg ctttaattct ttatgttgtc ggacgaacca tcccgccgga
     1081 tgatgaatca agccccaaca aggcagcatg gttaagctta ttttcaattc ttttgaccgt
     1141 tgttcctttt gtgctgacag caaaaaaatt tacggaagaa ggacctgttt ctttaaccgc
     1201 aggaatgatt accctcacat ttgacggaat cagccttctc atgacttcgg tcctgttagt
     1261 gttgtcggct atggttgtat tattttccgc tcaatacatg gtggcagaag atcatgaaga
     1321 gaaattctac ggtcaaatta tgattctgat gtccagtatt atggggttga caaccgcgac
     1381 agatatattc aacttgtatg tttggttcga attaatggct gtttcgactt acacattggt
     1441 cagtttttat cggaaccgta cagaatcatt ggaatccggc attaagtact tagttcaaag
     1501 tgcggctggt tcggtcttag taatattagg aacttcgatt ctttttggat tgacgggaga
     1561 agtcaaaatt tcggcaatca gagaaagcat tctaacctca agtccggcgg taatcgcagc
     1621 aacagcttgt ttactgatcg gctacggagt gaaatgtgca atcgtcccta tgcatttttg
     1681 gctgccggat gcccattctc aagctcccgc gggaatcagt gcattacttt ccggcattgt
     1741 gatcgaaacc ggtttaatcg cattgttacg cgctttgagt gccgtcagcc atgctcatct
     1801 cccgctcggg actatttttt tggtgatcgg atgtatcaat atgtttatcg gtactttttc
     1861 tgcccttcga cagaaggaaa tcaagcggat gcttgcattt tcttctgtca gccagatggg
     1921 atacatcctt ttgggaatcg gctttgcgat ttcttttccc gcaagcgctc aaaatgcaga
     1981 gggaattttt actcatatct tcaaccatgc tctaatgaag gggttagcct ttctttccgc
     2041 aggtgctctg gtatattgta taaaagtatc gaaaggaaac cacgaaccgt tattattgga
     2101 tgatctaaac ggggtttccg cgaaatatcc tttatattct ctggctttta caatctcaat
     2161 gctggcattg ggaggattgc ccttatttgc gggctttatt tcaaaatggc aaattctggc
     2221 aagcggaata gctgctcaaa agtcttgggt tgccttttta atagcacttg ccggtattaa
     2281 cagtgtttta tctttaggac attacgcacc tccgattaat cgtatttatc ggcatgtatc
     2341 aaacaataat gaagttgcca tttcaccaaa gtttcaacaa gcgatgatta ttccgatcat
     2401 catcttaacc gcagcagtca ttcttctcgg tttcttcccg ggtattaccg ccggaataac
     2461 caaagtcgca agcgaaactt tatatactat gttttattag gaaataaata ttatggatat
     2521 tttactgagt cctccgattg gttttctgat ctatgcgatt ataggcacct tattggtttt
     2581 ttacgggaag aagaccgctc cggtcgaaga tacgaaagac gatcaaaaaa ggactttata
     2641 cggaagcggt gaggttgcac cggaaaccag aggggttccg ggttataaac cttttttgct
     2701 gatttctctc ttttttgcac tacttcattt gggagtgctg attatgggca gcagcaatct
     2761 tagtgtgact tcgctggtgt atacggttgt attaatgtta ggtttattgg ctctgatgtt
     2821 aggctgattt ggcagacaga gatatatttt aaaacggaag aatcataaaa tgaacaagac
     2881 catcgatctt tcggatatcg atccgaatgc aaaaggctct gagaaaacaa ttgaaaacag
     2941 aattcgtaat tgggcgagaa taaacagccc ttgggcaatt cattataaca gcggatcttg
     3001 taacggttgc gatatcgaac tattaacttt gattaccccc cgttatgatg ttgaaagatt
     3061 cggagtgaaa ttacagggga gccctcgtca tgccgatatt ttaatcgtca ccggttccgt
     3121 gactcttcaa tcaaaagatc gcctcattcg aatatataat caaatgccgg aaccgaaatt
     3181 cgttttggga ttgggttcct gcgcgatcag cggaggtgta ttccaaaatt gttatagtgt
     3241 tgttcaaggg ggagtgagca acgttattcc ggtagatgtc tttgtcccgg gctgtcctcc
     3301 cagaccggaa gcaatgatta atggattgat catcctcctc gatattgcca gagggaagcg
     3361 acagaaatcg gattacagtc agcctatgat tatcccctca cccgaacaag attctgctca
     3421 gacagcggat aaaaaagaaa gtgaagccga ataatggaaa aagaggaatt actgaatcag
     3481 tttcaggaaa tatttaccga tttggcaata aaaactgaaa ttccggcgat acaaccgaat
     3541 cgggtggatg tttatattaa ttcgaacgat ttattaaaag cggttcaaaa attggtcgaa
     3601 catgattggg gatatttaat tactatctcg gcttacgata cggctgacga aaacggtacc
     3661 ccgcaaatcg gattggttta ccaattcgga gaacaaaatg tgatcggaac attacgtttc
     3721 tttttgccgt attcaaactt aacggtcgaa tcaatatgcc cgattattcc ttcggcaacc
     3781 ctttatgaaa gggaatgtat ggaattattt ggtattgata tccgaaatac accggatcgc
     3841 tccaaactca tattaccgga aaattggccg gatggagttt ttccgcttcg gaaatctttt
     3901 tccggattac aggataaccc ggaaacaaaa tctggagaag aataatgagc gatgaaacag
     3961 taaacaactt caataataat ggtcagcaaa agtatatcgt tcctatcgga ccgcaacatc
     4021 cggcgttgaa ggaaccggga cattttgaat tcaccgtaga tggggaaatt gtcgtagg
//
LOCUS       DAOMIV010000142         4074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22834_length_4074_cov_3.933814, whole genome shotgun sequence.
ACCESSION   DAOMIV010000142 DAOMIV010000000
VERSION     DAOMIV010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22834_length_4074_cov_3.933814"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /gene="holA"
                     /locus_tag="PKV59_04405"
     CDS             <1..796
                     /gene="holA"
                     /locus_tag="PKV59_04405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQP46029.1"
                     /translation="NPLALASGKDGNKKLLRLLESVPPTTHIVLVITDDFERKDWLIF
                     GKTSFLRKWAEKNSEKAEIIEKRKPAVGMMRDWIIKKTIAMGGQIEPQAAQILVTAVG
                     NDTRAAKQELEKLLLYVNYGRPIDSMDIQELVTGSASVSIFDMVDALASGNAKEAFRT
                     LDLLMQDQEIPVLFAMIIRQFRLLIQTREIMDEKGNIATVQDELNQHPFVAEKLWRQA
                     SRFRFLQLKSIYRKLVEMDFAFKTSQPDSRAALDVLIADISLMVQD"
     gene            complement(780..1895)
                     /locus_tag="PKV59_04410"
     CDS             complement(780..1895)
                     /locus_tag="PKV59_04410"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HQP46030.1"
                     /translation="MDHKILTILLIVFTVITGCSSSVWADQEQKVINPLTGIETDPNW
                     LSYPPFIIPYTKFPDKFRSSAGLSFAPWVFEMYSGDGESRPMALFYGSIPESTTGVTP
                     YIGSVSSAVLGLESLRKQYMAVLITAGNPDLVKKAGISNLETWYGESGSEKYPDLPVQ
                     QFIEIMEKWKKRLGSPDTEGISLPFSAAIPEGSRSGKTLFIRYAVFNQILWEFDEADG
                     LYHRSQNSVSDPNPVKDIDVLNNQQITAQNVILLFADHSLIEDGVNFTVNFNYVKKNP
                     ALIFRDGLCYQAFWTTKAEEYEIQNQKLRPIRFLEADGDAFHLKPGKSWVHVVQINNP
                     VYEIAEPTGNQTEDGSGHWKVPYISVKPNEKNLNPVP"
     gene            complement(2002..2073)
                     /locus_tag="PKV59_04415"
     tRNA            complement(2002..2073)
                     /locus_tag="PKV59_04415"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2039..2041),aa:Asn,seq:gtt)
     gene            2469..3413
                     /gene="ppk2"
                     /locus_tag="PKV59_04420"
     CDS             2469..3413
                     /gene="ppk2"
                     /locus_tag="PKV59_04420"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HQP46031.1"
                     /translation="MAKEKKKKKTKIDQKIGSGQTELSNEKKENEKKKDVISAMPTSK
                     STSIPCGMPYTKPPLSKKEYEKELEKLQIELVKLQGWIKEKGLKVVVIFEGRDAAGKG
                     GVIKRITELLNPRIVRVVALPVPTEKEKTQWYFQRYVAQLPAAGEMVLFDRSWYNRAG
                     VERVMGFCTEEEVTEFFRSAPEFERMLIRSGIILIKYWFSVSDEEQEKRFQDRINDIT
                     KRWKLSPMDLAARSKWVEYSKAKDEMFYYTDTKQSPWYVVNADDKQKARLNCIHHLLS
                     LIPYEDLTPEPVVLPPRQSDEGYVRPPISDQTFVPEIY"
     gene            3629..>4074
                     /locus_tag="PKV59_04425"
     CDS             3629..>4074
                     /locus_tag="PKV59_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46032.1"
                     /translation="MNARKIPFLLFLFVIFLIPFSAEAAGDQTNPNLFPVTVAKNIDP
                     GLIDAINEGDSGGEIGLALIGSDTTVGYYKKCSTDAYFYFIVANNSSATVGTVLPRWA
                     KIDGRAYFTDSSAYLCKVLAAGATPSNNFADADSGSACILQNNQLRV"
BASE COUNT         1271 a          862 c          875 g         1066 t
ORIGIN      
        1 caacccgctt gctctggcaa gcggaaaaga cggaaataaa aaactgctcc gcttgttgga
       61 gtccgttcct cccacgaccc atattgtttt agttattacg gatgatttcg agcgaaaaga
      121 ctggctgatt tttggaaaga cctcattcct acggaaatgg gccgaaaaaa acagtgagaa
      181 ggctgaaatt attgaaaaac gaaagccggc tgtcggaatg atgagagatt ggattataaa
      241 gaaaacaatc gctatgggag gacaaatcga accgcaagcc gctcagatat tagtcactgc
      301 ggtcggaaat gatacgcggg cagccaaaca ggaactcgag aaactgcttc tatatgtaaa
      361 ttacggcaga ccgattgaca gtatggatat tcaagaattg gttaccggtt cagcatccgt
      421 cagtattttt gatatggtcg atgctttggc atcgggaaat gcaaaagagg cgttccgaac
      481 tttggactta ttgatgcaag atcaggaaat tccggtttta tttgccatga ttatcagaca
      541 gtttcgctta ttgatccaaa cccgtgaaat tatggatgaa aaaggaaata tcgcaaccgt
      601 tcaagatgaa ctaaaccaac atccgtttgt tgccgaaaaa ctctggcggc aggcaagtcg
      661 gttccgattc cttcaattaa aatctattta tcggaaattg gttgaaatgg attttgcatt
      721 taaaacaagc caaccagatt cgagagccgc cttggatgta cttatcgccg atatttccct
      781 tatggtacag gattaagatt cttttcgttg ggtttaacag atatatacgg gaccttccag
      841 tgtccgctgc cgtcttctgt ctgatttccc gtcggttcgg caatttcata aaccggatta
      901 tttatctgaa caacgtggac ccagcttttc cccggtttca gatgaaacgc atccccgtca
      961 gcctctaaaa agcgaatcgg gcgtaacttt tggttttgaa tttcatattc ttccgctttg
     1021 gttgtccaaa aagcctgata acacaatcca tcccgaaaaa ttaacgccgg attcttttta
     1081 acataattga aattaacagt gaaattaaca ccgtcttcaa ttaatgaatg atccgcaaag
     1141 agcaaaatga cattttgagc agtaatttgc tgattattaa gaacatcgat atccttaacc
     1201 ggattcggat cggaaaccga gttttgagat cggtgatata aaccgtcggc ttcgtcaaat
     1261 tcccaaagaa tttgattaaa aacggcataa cggatgaaca gtgtctttcc cgaccggctg
     1321 ccttccggaa tcgccgctga aaagggaagc gatattccct ccgtatcggg agaacccagt
     1381 cgctttttcc atttttccat aatttcaatg aactgttgaa ccggcaaatc cggatatttt
     1441 tccgaaccgg attcaccgta ccaagtctca aggttagata ttcccgcttt cttgacaaga
     1501 tcgggatttc cggcggttat caataccgcc atatattgct ttcgtagcga ttctaatccg
     1561 agaacagccg aagatacgga accgatataa ggtgtaacgc cggttgtcga ttccggaata
     1621 ctcccataaa acagagccat cggtcgcgat tccccgtctc cggagtacat ttcaaaaacc
     1681 caaggagcaa agctcaatcc cgcggaagat ctgaatttat ccggaaattt tgtgtaagga
     1741 ataataaacg gaggataact taaccaatta ggatcagtct cgattccggt cagcggattg
     1801 atcacctttt gttcctgatc cgcccaaaca ctggaagaac agcctgtaat caccgtgaat
     1861 acaatcaaca aaattgtcaa aatcttatgg tccattttgg ttctttctat catgaaatta
     1921 aataaccttc gctcgcatct aagaattgta caacagattc tttaaacaca aatacctccg
     1981 actgatcgga ggtatttttt gctcctcgac ctggattcga accgggaacc taacggttaa
     2041 cagccgctcg ctctgccgtt gagctatcga ggatcaacgc ctaaattata gcaactttta
     2101 aaagcctgtc aaggacgacc ggcgattctg tgttgttatt cagcaataat gtcatcccga
     2161 tgaaatacag gcgattttgt catcccttcc gaaaaataaa ggatccccaa cggaatgaaa
     2221 atcaaaatgg atatcaaaga agcacgacaa atcacagtta ttgaacaagc catagaaagg
     2281 aagtttagcg accgagatgc tgcacagcta ttagagtggt cggcagcata tagtaaatga
     2341 tttttcactt taccgctgcg agaaagcgct tatctttttt tctgatcgta tcggaagtac
     2401 aattaaatca tcagcagacc ggataataaa taagtcattt ttctttccca atgaaagtcg
     2461 aggctattat ggcaaaagaa aaaaagaaga aaaaaacgaa aatcgatcag aaaataggat
     2521 cgggacaaac agaactatca aatgaaaaaa aggaaaacga aaagaaaaaa gacgtaattt
     2581 ccgcaatgcc aacctcgaaa agcacaagta tcccgtgcgg tatgccttac acaaaacctc
     2641 ccctcagcaa aaaagaatat gaaaaagaac tggaaaaatt gcaaatcgag ctcgtaaaac
     2701 tacaggggtg gattaaggaa aagggactga aagtcgtcgt aatctttgaa ggacgggacg
     2761 cagccggaaa aggcggagtg attaaaagga ttaccgagtt actaaacccg cggattgttc
     2821 gtgtcgtcgc cttaccggtc ccgaccgaaa aggaaaaaac gcaatggtat tttcaacgat
     2881 atgtcgctca attgccagcg gcgggagaga tggttttgtt tgatcgaagt tggtataacc
     2941 gggcgggagt ggaacgggtt atgggatttt gcaccgaaga agaggtaacg gaatttttcc
     3001 gcagtgcccc cgaattcgaa agaatgctga ttcgctccgg catcattctg atcaaatatt
     3061 ggttctccgt aagtgacgaa gaacaggaaa aacgattcca ggatcggatt aatgatatta
     3121 ccaaacgttg gaaactcagc ccgatggatt tggcggctcg cagcaaatgg gtggaatatt
     3181 ctaaagcaaa agatgaaatg ttttattaca ccgacacaaa acaatccccc tggtatgtgg
     3241 tcaacgccga tgataaacag aaagcgcgcc tgaactgtat tcatcatctg ctgagcctga
     3301 tcccttatga agatttgact ccggagccgg ttgttttgcc gccccgacag tctgatgagg
     3361 gatatgtcag gccgcctatc agtgatcaga cattcgttcc tgaaatttac tgatttatac
     3421 taccgcatat agttgcgaaa aaaataaacg caaaagattc tgtttgttta aacaaatgga
     3481 gtcttttctt tctaatccga gccgtccgat tataaaaata aacgattgaa attatcgatt
     3541 gaacaaatat tttcataatt ggcatgaatg atgcacattt tatgattaat aagattttaa
     3601 aatattccat caaataagga tgcagcttat gaacgcccga aaaattcctt ttctcctttt
     3661 tctttttgtc atattcctga tccctttttc cgctgaagcc gccggggatc agacaaaccc
     3721 aaatttattt ccggtcacag ttgcaaaaaa tatcgatccg ggactcattg atgcgatcaa
     3781 tgaaggagat tccggaggcg aaatcgggtt ggcccttatc ggaagtgaca ccacagtagg
     3841 atactataaa aaatgttcga ccgatgcata cttttatttc atcgttgcga acaacagcag
     3901 cgcaactgtg ggtacggttc tgccgagatg ggctaaaata gacggtcggg catattttac
     3961 agacagtagc gcttatctct gcaaggtact tgcagcaggc gctacaccaa gtaataattt
     4021 cgccgatgcc gacagcggat cggcttgcat tcttcaaaac aatcaactga gggt
//
LOCUS       DAOMIV010000143         4067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22882_length_4067_cov_4.345962, whole genome shotgun sequence.
ACCESSION   DAOMIV010000143 DAOMIV010000000
VERSION     DAOMIV010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22882_length_4067_cov_4.345962"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PKV59_04430"
     CDS             <1..742
                     /locus_tag="PKV59_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46033.1"
                     /translation="NGTVKSDKTPKADMSYLPNGMTYQEFELASPPLPAPLRLNPLEG
                     SLDDHLNKRKMTTLSERTNFSYGFYGETYSTSLEYDTEGNRDPYYPTRSNLTIYSNGY
                     EFYSFWFPHVESPFFYNWGIVNYGGDWYLPIRMTVDINPDPDITELIYSDRTIMSQMN
                     FDKSFNYIRSFGFSIIGYNLFYFYQRPGGYGIAINRVDYPLNFDEIIFGLVGDYQDLN
                     PFYSDTLITFFGLRGNTWYYVELTAPEE"
     gene            923..2344
                     /locus_tag="PKV59_04435"
     CDS             923..2344
                     /locus_tag="PKV59_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HQP46034.1"
                     /translation="MSLKIKFGTDGWRAYIAEDYTFDNCRRCAQGFANYLLKKDKKGE
                     CVVVGYDKRFHSENFAAACAEVMAGNGFNVLLTQEATPTPVISYAVVDKKAIGAINIT
                     ASHNPPSDNGFKVRDATGGAIDPQGLLEIEAGIPDDMSDVRRIPLNDGLESGRIEFFD
                     ASVNYISHLKDLIDLEPIRKSGLKILVDPMWGNGSGWFKRLLGGDKTEIIEIHNERNP
                     IFPEMKRPEPIPPNIDVALKKTVELGADVLLITDGDADRCGLGDENGVFLNQLQVMAL
                     LTLYLLEIRKERGAIVKTLSTTTMLNKLAKIYGVPIYETGVGFKYVAPKFKETDAIIG
                     GEESGGYAFRGNVPERDGILANLYFLDFMVKTGKTPSQLLQWLFEIVGPHYYERIDTS
                     FKGNAEEKKKFILDHIPEKLGGIKVTGLDTTDGFKFMMEDGGWMLIRFSGTEPIIRVY
                     TETTHEDCVDAILKDGLKVAGIE"
     gene            2328..3125
                     /locus_tag="PKV59_04440"
     CDS             2328..3125
                     /locus_tag="PKV59_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQP46035.1"
                     /translation="MRELNNTDYWIDTHCHLNLPDFLPDQAAVIDRARQANVRRMIVP
                     GIDPESSETAKKMAEAYPAVFFAVGVHPNESADWDDSRLQDLKILATHPKCVAVGEIG
                     LDFYREFCPPPQQLRSLHAQLELAEEIGKPVILHCRQAFEVLWPILQDWHRHHSKNIG
                     VLHAFEESPEAAAEVTAQGFYVGIGGAYTYKKKKHLSEVLKTVSLDKILLETDSPYLT
                     PTPYRGQRNEPMYIPLIAEKVASTRNISTEALSEIVRENNLRLFGNL"
     gene            3364..3696
                     /gene="trxA"
                     /locus_tag="PKV59_04445"
     CDS             3364..3696
                     /gene="trxA"
                     /locus_tag="PKV59_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQP46036.1"
                     /translation="MINEPIHVTDDAFEKTVLKSEIPVVVDFWASWCGPCRMVLPLME
                     ELAKEYAGKLIVAEVNTDENSARAMDYNVTSIPTLLFIKNGEVKYRHTGTLSKAKLEE
                     YANNLLAA"
     gene            complement(3765..>4067)
                     /locus_tag="PKV59_04450"
     CDS             complement(3765..>4067)
                     /locus_tag="PKV59_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQP46037.1"
                     /translation="SVLFHDWMYPSRMYFTDKLVGMGAQIVLCDPHRCIVQGPTRLYP
                     EKLESPDIRAGMALLLATLAVPGKSVIRNISQIERGYERVDEKLRALGARIERVEA"
BASE COUNT         1161 a          847 c          940 g         1119 t
ORIGIN      
        1 gaatgggacg gtaaaatctg ataaaacccc gaaggctgat atgagttatt tgccaaacgg
       61 aatgacctat caggaatttg aacttgcttc acctccttta ccggctcctc tccggctcaa
      121 tccgctggaa ggatcactcg acgaccattt aaataagcgt aaaatgacaa ccctcagcga
      181 acggacgaat ttttcctatg gtttttacgg tgaaacctat tccacttcat tagaatatga
      241 tactgaaggg aaccgggatc cctattaccc gacacgttcc aacctaacaa tctattccaa
      301 cggatatgaa ttttacagtt tttggttccc gcatgtcgaa agtccgtttt tttataattg
      361 gggaattgta aattacggcg gggattggta tttgccgatc cggatgacgg ttgatatcaa
      421 tccggatccc gatatcacgg aattgatcta ttctgaccgc acgattatga gtcaaatgaa
      481 ttttgataag tcattcaact acatccgttc tttcggcttt tcgatcattg gttacaattt
      541 attctatttt tatcaacgtc cagggggata tgggatagcg atcaatcggg tggattatcc
      601 actcaatttt gatgagatta ttttcggatt ggtcggtgat tatcaggatt taaacccatt
      661 ttattcggat acgctgatca ctttcttcgg cttgcgcggg aatacatggt actatgttga
      721 gctgaccgca ccggaagagt aatagtgtag taatacagga ttgaagtatg gcgaaaggat
      781 acggatcgga aaagcttgtt tcgttttcct ttcgttcata agttttaatg atttttattg
      841 atttttttct cggacggcac aagtataatt aatcagaaat ttggaaaaat tctgctatct
      901 cgaataacta cggaggaatc gcatgagtct gaaaatcaaa ttcggtacgg atggctggcg
      961 ggcatatatc gccgaagatt atacatttga taattgccgg cgctgcgccc aaggtttcgc
     1021 gaattatttg ttgaaaaagg acaaaaaagg agaatgcgtt gttgtcggat atgacaagcg
     1081 ttttcattcg gaaaatttcg cagccgcctg cgcggaagtc atggcgggaa acggatttaa
     1141 tgtccttttg actcaggaag cgactccgac acccgttatt tcctatgcgg tcgtcgataa
     1201 aaaagcgatc ggagctatca atattacagc ttcccataac cctccttcgg ataacggatt
     1261 caaagtccga gatgcgaccg gtggtgcgat tgacccgcag gggcttttgg aaatagaagc
     1321 cggtataccg gatgatatga gcgatgtcag acgaattccg ctgaatgacg gtctggaatc
     1381 cggaagaatt gaattcttcg acgcttctgt caactatatc agtcacctaa aagatctgat
     1441 tgatttggaa ccgattcgaa aatccggttt aaaaatcctc gtcgatccga tgtggggaaa
     1501 cggatcgggc tggttcaagc gattgctggg tggagataag acagaaatca ttgaaattca
     1561 caatgaacga aatccgatct ttccggaaat gaaacgcccc gagcctattc cgccgaatat
     1621 tgatgtggct ctgaaaaaga cggtcgaatt gggtgccgat gttcttctga tcaccgacgg
     1681 ggatgcagat cgctgcggtt tgggagatga aaacggcgtt ttcttgaacc aactgcaggt
     1741 aatggctctt ttaaccctat atttactcga aattcgcaaa gagcggggag cgattgtcaa
     1801 gaccctttcc acaacaacga tgctcaataa attggctaag atatatggtg tcccgattta
     1861 tgaaaccggt gtaggattca aatatgtcgc gcctaaattt aaggagacag atgcaatcat
     1921 tgggggagaa gaatcgggcg gatatgcctt ccgcgggaat gttccggaac gggatgggat
     1981 cctcgctaat ctttatttcc ttgattttat ggtaaaaacc ggtaaaaccc catctcaatt
     2041 gcttcaatgg ttgtttgaaa ttgtcggtcc ccattattat gaacgaatcg atacctcctt
     2101 taaaggaaac gccgaagaaa agaaaaaatt tattctcgat catattccgg aaaaattagg
     2161 cgggattaaa gtgaccggat tggataccac ggatggcttt aaattcatga tggaagacgg
     2221 cggttggatg ttgattcgat tttccggtac cgaaccgatt attcgggttt atacggaaac
     2281 tactcatgaa gattgcgtag acgcgatttt gaaagacgga ttaaaagttg cgggaattga
     2341 ataataccga ttattggatc gatactcatt gccatctgaa tctgccggac tttttgcctg
     2401 atcaagcagc ggtcatcgac cgagcccggc aggcaaatgt ccgcaggatg attgtgccgg
     2461 ggatcgatcc ggaaagcagt gaaacagcaa aaaaaatggc agaagcgtat cctgccgttt
     2521 tttttgcggt cggcgttcat cccaatgaat cagccgattg ggatgattcc cggctccagg
     2581 atctgaaaat tttggctacc catccgaaat gtgtcgccgt cggagaaatc ggattggatt
     2641 tctaccgtga attttgtcct cccccgcaac agttgcgatc gctccatgcc caattggaat
     2701 tggcggaaga aatcggtaag cctgttatcc tccattgccg tcaggctttt gaagtgcttt
     2761 ggccgatatt gcaagattgg catcgacatc attcgaaaaa tatcggggtt ctgcatgcat
     2821 tcgaagaatc accggaagcc gcagcagagg tgaccgcgca aggtttctat gtgggaatcg
     2881 gcggagcgta cacctataag aaaaagaaac atctttcgga ggttctgaaa acggtcagtt
     2941 tggataaaat tttacttgag accgactcgc cttatctgac acccactcct tatcgcgggc
     3001 aacggaatga accgatgtac atcccattga ttgcggaaaa agtggcgtcg actcggaata
     3061 tctcaaccga agcgctttcc gaaatcgtcc gggaaaacaa cctcagatta ttcggaaatt
     3121 tataatcgga ttttgaaaaa attctgactt ttgacaatcc tttttcatat tccgttgaag
     3181 agtatgacgg aataagtcaa acagccatcg atctaacttt tttctgcatt ttttgcattt
     3241 gcaaaataat taaagatttg ttaatcctga taacccaata aaaaaatacg gtaaaatttg
     3301 aaagtgagtt cattttaagc tgaatttgat tcagcgattt ggaaggcaga aaggtaaaat
     3361 atcatgatca atgaaccgat tcacgttacg gatgacgctt ttgaaaaaac agtccttaaa
     3421 tcagaaatcc ccgttgtggt tgatttttgg gcgtcttggt gcggaccttg cagaatggtt
     3481 ctccctttga tggaagaact tgcaaaggaa tatgccggta aattaatcgt tgcggaagtc
     3541 aatacggatg aaaacagcgc ccgggcgatg gactataacg tcacatctat tccgaccctc
     3601 ttattcatta aaaacggtga agtaaaatat cgccataccg gaactttatc caaggcgaaa
     3661 ttggaagaat acgccaataa tttattagcc gcataatttc aacacgaaat gaaaaggagc
     3721 tgtttcgaag cagctccttt ttttttatct ttcaaacaac tttttcaggc ttcgacccgc
     3781 tcgatgcgtg ctcctaaagc ccgcagtttt tcatccactc gctcatatcc gcgttcgatt
     3841 tgagaaatat tgcggataac ggattttccc ggaacagcca acgtcgccag caacaacgcc
     3901 atccccgcac ggatatccgg cgattccaat ttttcggggt acaaacgggt aggtccctga
     3961 acgatacaac gatgcggatc gcagagaacg atttgagcgc ccataccgac caatttatcg
     4021 gtaaaataca tacggctcgg atacatccaa tcatgaaaga ggacgct
//
LOCUS       DAOMIV010000144         4065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_22899_length_4065_cov_3.701995, whole genome shotgun sequence.
ACCESSION   DAOMIV010000144 DAOMIV010000000
VERSION     DAOMIV010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22899_length_4065_cov_3.701995"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PKV59_04455"
     CDS             <1..813
                     /locus_tag="PKV59_04455"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HQP46038.1"
                     /translation="EQPYYSLHRIWRSISLFNADLNLDEYEPTQEYPLFVKPSKPITP
                     EMLINVLKDTYEGTDLDEYGKTEIPYGTIIDPVTGHYRYSPAWSKSRIIGCPQTITSW
                     VTQSRSWMPNEIGGLIWAGLAATASSPHIPFYACHKDTPSAYKIGIAGDNSVYLPDSA
                     YWLFENIGNIMNLFYDGTVDLVKPVWEDFDHRRYEFQNSIEQIALNLYKEDKQKMIDF
                     LTSYSNGVALEALEVGQDMLAKLFTRLAMVNNPQTTRGYENPAEWKKSGFIY"
     gene            953..1846
                     /locus_tag="PKV59_04460"
     CDS             953..1846
                     /locus_tag="PKV59_04460"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQP46039.1"
                     /translation="MILRAHHIGIAVEKLDQAISTYDKLFGYKATDIRNNQGKGFQLD
                     ARIMMPNRTWLHLVENWNPESRVNKFLQQHGEGLEHIALETTDIEEEVAYLRSIGVPV
                     FEDKIFNAADGFEAFIYPDAAIGFTVELIQPHVTSYYWQPEKVSNPNLLGLQHIGVAV
                     KNVVEAANKFEKLFRIKSSDLRTDQHYGTQKDIMIEPGNDRLWLHLVESTDPENRVTQ
                     FMAQHGEGLEHLAFEVNDIRRAVKTVKKAGVSLTANKIYLDREDGFEAFVDSKNNHGV
                     TVELIEPYITSRGYRYKVEDF"
     gene            1925..2629
                     /locus_tag="PKV59_04465"
     CDS             1925..2629
                     /locus_tag="PKV59_04465"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HQP46040.1"
                     /translation="MKILVLNPNTSEAVTQRIAETAKKAAYPDTEIVVTGIPHGPESL
                     ESFYDEAIATPYILQSVKNANEAGYDAVINAAFCDPGIEALKEISDIPVFGLEECSFS
                     AALLFGNKFAILTEQKHKEAVKSREVRKHGLESRFAGVKALNMGVVEIANNPLKVKEA
                     GIKAARELIENGAEVIILGCSSMAGYSEDLEKELKVPIIDPVATTVKMAEAFTALGLR
                     HAKIGLYSKPKIQKMN"
     gene            2686..3693
                     /locus_tag="PKV59_04470"
     CDS             2686..3693
                     /locus_tag="PKV59_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQP46041.1"
                     /translation="MTKVVIADSGTKEQIANWHDFGIRYELDVVMPSTPDEEELFRLL
                     PDAEVLVTQKVTIDERMMNAAPKLRLIQKMGSRADNIDLEAAKRRRINVSVVSLPGSV
                     AVAEHAMMLILACAKKLILAHELTVKGAYRDLGIEPKLTSQKSHGFQWMKIPDLQELN
                     GSTLGIFGFGDIGNEIAKRAHAFEMEILYFKRHPLPEKMEEELGVKYVSKEELFKRSD
                     FLVMISPLTPETEKAAGAKEFALMKPTAYFINCCRGGVVDEEALVETLKNHRIAGAGL
                     DVFVYEPIPYDHPLLSLDNVTLTPHIAGGKGNAAERQIYTVLENIQKYLKNEPLKNLI
                     C"
     gene            3792..>4065
                     /locus_tag="PKV59_04475"
     CDS             3792..>4065
                     /locus_tag="PKV59_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46042.1"
                     /translation="MVEAIKIGNISVMPGEMKRGAIAIGKNMYGIERELPVIVYRGLH
                     EGKCLWITGSTHGDEPEGPFSIKLLMDRPELDPAKMSGTLVLVPALN"
BASE COUNT         1340 a          678 c          912 g         1135 t
ORIGIN      
        1 gaacagcctt attatagtct tcatcgaatt tggagaagca tcagtttgtt caacgcagat
       61 ttaaatttag atgaatatga acctactcaa gagtatccgc tttttgttaa accttccaaa
      121 cccataactc ctgagatgct aattaatgtt ttaaaggata cttatgaagg aactgatttg
      181 gatgaatacg gaaaaactga aataccctat gggactatta tagatcccgt taccgggcat
      241 tatcgatatt cgccggcttg gagtaaatcc cgaatcatcg gatgtccgca aacgattacc
      301 tcttgggtaa cccaatctcg cagttggatg ccgaatgaaa tcggtgggct gatctgggcg
      361 gggttagcag ccacggcgag cagtccgcat atcccatttt atgcctgtca taaagatact
      421 ccttcggcat ataagattgg catagccgga gataacagcg tttatttacc tgattcagca
      481 tattggcttt ttgaaaatat cgggaacatc atgaatttat tttacgatgg cacggttgat
      541 ttagtgaaac cggtctggga agattttgat cataggagat atgaatttca aaattccatc
      601 gaacaaatcg ctttgaacct ctacaaagaa gataagcaaa aaatgattga tttcctgact
      661 tcctattcaa acggcgttgc tcttgaagca ttggaagtcg gtcaggatat gttagcgaag
      721 ttgtttacaa gattagcaat ggtaaacaat cctcagacga cgcgtggata tgaaaatccg
      781 gctgaatgga aaaaatcagg ttttatttat tagtcatgta aaagaatcgg ttttgatatg
      841 aatatccaat cggaaacaaa taatgtctat acctgaaaaa aagcgaatat cttagataaa
      901 aaataataac gcggaagatt gatttttcaa aaatatattt aaggaggaac caatgatttt
      961 aagagctcat catatcggga ttgcggtgga gaaactggat caggctatat ctacttatga
     1021 taagttgttc ggatataaag caacggatat caggaataat caaggaaaag gatttcaatt
     1081 ggatgcaaga atcatgatgc cgaatcgaac atggctgcac ttagttgaaa attggaatcc
     1141 ggaatctcga gtaaacaaat tcttacagca acatggtgaa ggattggaac atatcgctct
     1201 tgagactact gatattgaag aagaagtagc gtatttacga tcaataggtg tcccggtatt
     1261 tgaagataaa atttttaatg cagcggatgg attcgaagct tttatctatc cggatgcggc
     1321 catcggtttt acggtcgaat tgattcagcc tcacgttaca agttattatt ggcagccgga
     1381 aaaagtttca aatccgaact tattgggatt gcagcatatc ggtgttgcgg tgaaaaatgt
     1441 agttgaggcg gcaaataagt ttgaaaaact ttttagaatc aaatcctccg atctgcggac
     1501 agatcaacat tacggaactc agaaagatat catgattgaa ccgggaaatg accgattatg
     1561 gcttcatctt gttgaaagta ccgatccgga aaatcgtgta acacaattta tggctcaaca
     1621 tggagagggg ctggaacatc tggcatttga agtaaatgat attcggagag ctgtaaaaac
     1681 agtcaaaaaa gcgggtgttt ctctgacagc aaataaaatt tatctcgatc gagaagatgg
     1741 atttgaagcc tttgttgatt caaaaaataa tcatggggtc accgtagaac taatcgaacc
     1801 atatattaca agtcgcggat atcgctataa agttgaagat ttttaagtgt tttctataat
     1861 tttcaatttt aaataaatgg ttgtttgctt taagagatat tgttttataa ggagccgaat
     1921 aaaaatgaaa attttagttt tgaatccgaa cacgtcggaa gcggttactc aaagaatcgc
     1981 ggaaacagcc aaaaaagcag cttatccgga taccgaaatt gttgtgaccg gtattccgca
     2041 tggaccggag tcacttgagt ctttctatga tgaagcgata gccactcctt atatcctcca
     2101 atctgtgaaa aatgcaaatg aagccggtta tgatgctgtc attaatgccg ctttttgcga
     2161 tcccggaatt gaggctttaa aagaaatttc ggatataccc gttttcggat tggaagaatg
     2221 ttccttttct gctgcccttc tttttggaaa taaatttgcg attttgacgg aacaaaagca
     2281 taaagaagct gttaagtcac gggaagttag gaaacatgga ttagaaagtc gtttcgccgg
     2341 tgtaaaggct ttaaacatgg gtgtcgtgga aatagcgaac aaccctctga aagttaaaga
     2401 agccggaata aaagcagcca gagaactgat cgaaaacggt gcggaagtca ttatcttggg
     2461 ctgttcatcg atggcgggtt attcagaaga tttagaaaaa gagttaaaag tcccgatcat
     2521 tgatcctgtt gcgacaacgg ttaaaatggc cgaggctttt actgctttgg gtctgagaca
     2581 tgcaaaaata ggactttatt cgaagccgaa gatacaaaaa atgaattgac agaaatgtaa
     2641 tttaaagaaa ttcatgtaat taaaagaaag aggataaacc gacacatgac aaaagtggtt
     2701 attgccgata gcggtacaaa agaacaaatt gccaattggc atgattttgg aatccgatat
     2761 gaattggatg tggttatgcc ctcaactccg gatgaagaag aattattccg cctattgccg
     2821 gatgccgaag ttttagtcac tcaaaaggta actattgatg aacgtatgat gaatgccgca
     2881 ccgaaattaa ggcttattca aaaaatgggg agtcgggctg ataatatcga tttagaggcg
     2941 gcaaagcgac gacgaattaa tgtttccgtt gtctcattac cgggatccgt tgcggtagct
     3001 gaacatgcaa tgatgctgat cttagcatgt gcaaagaaac tgatcttggc tcatgaactg
     3061 accgtaaaag gtgcttatcg tgatttagga attgaaccga aacttacctc acaaaaatct
     3121 catggttttc aatggatgaa aattcccgat ttgcaggaat taaacggatc aacgttgggc
     3181 atttttggct ttggcgatat cggtaatgaa attgctaaac gagcccatgc ctttgaaatg
     3241 gagatacttt actttaagag acatccatta ccagagaaaa tggaagaaga attgggtgtc
     3301 aaatatgtca gtaaagaaga attgttcaaa cggtctgatt tcttggtgat gatcagtccc
     3361 ttgacaccgg agacagaaaa agctgcgggt gcgaaagaat tcgctttaat gaaaccgaca
     3421 gcttatttta ttaattgttg ccgtgggggt gtcgtcgatg aagaagcact tgttgaaacg
     3481 ctaaaaaatc atcgaatcgc aggagccggt ttggacgttt ttgtctatga gcccattcct
     3541 tatgatcatc cgttactttc tttagataat gtgacgttaa ctccccatat tgccggagga
     3601 aaaggaaatg cggctgagcg tcaaatttat acggtcttag aaaacattca aaaatattta
     3661 aaaaatgaac ccctaaagaa tttaatttgc taatttgaaa taaggaatca gatcataaaa
     3721 agataaaaag tcttatctga tttccatgca aaaagaagat atttaaaaat cgaaaaagag
     3781 gagaaaatag tatggtcgaa gcaataaaaa tcgggaatat ttcagtgatg cccggcgaaa
     3841 tgaagagagg cgcaatagca atcggtaaaa atatgtatgg aattgaaaga gaattgccgg
     3901 tcattgtcta ccgcggcttg cacgaaggaa aatgtttatg gattacaggc agtactcacg
     3961 gtgatgagcc ggaaggacct ttttcaatta agcttttaat ggataggcct gaattggatc
     4021 ccgccaaaat gtccggcaca ttggtacttg tacctgcttt gaatg
//
LOCUS       DAOMIV010000145         4045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23057_length_4045_cov_4.446115, whole genome shotgun sequence.
ACCESSION   DAOMIV010000145 DAOMIV010000000
VERSION     DAOMIV010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23057_length_4045_cov_4.446115"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="PKV59_04480"
     CDS             complement(<1..1609)
                     /locus_tag="PKV59_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQP46043.1"
                     /translation="MPYPAVDLKKVRTVPLPARHNRVATDDFIFPSTPVAEFDSPDLK
                     EVAERILAARRAGRPLIWMQGAHVIKSGLAPILIDLLEKGYVQHVASNGAATIHDFEI
                     ALLGHTSEDVADSIKDGTFGMAEETGLLMNTAIQNGAKRGMGIGESLGELIGTDDRFA
                     QYRDVSVLYHTWRLGIPYTVHVAVGTDIIHQHPAVDFAAIGWGSGQDFKIFCKSVCDL
                     EGGVFLNVGSAVIGPEVFLKALSIARNLGNPLKVFTTANFDLIDLGDYRAPLGEDKVQ
                     YYYRPRKNVVNRPVMLGGKGFHICGNHKVTLPNLYQLLTANGETFPVESKRISLPMTE
                     MIDPSVRLILDEAIRLHPQIENVKEPIAQAYQLIRRSYSCGGTLFVAGNGGSMADALH
                     ISGELSKAFVVKRHMPAALAARLKKSEEGSVLAENLEEAQRVMVLGLNPVLRSAIEND
                     FQHPNLAVAQELYANAKPGDVMISISTSGNAENLHYAALLAKELGVYVICMTGSDGGK
                     MRKDADILIAVNQSQTDEIQNLHILIYH"
     gene            complement(1829..2779)
                     /locus_tag="PKV59_04485"
     CDS             complement(1829..2779)
                     /locus_tag="PKV59_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46044.1"
                     /translation="MKKVTLIIVLAMLLSVCSVAMAAEKSYTIALITMDSMDQHWLKV
                     KAGAEAAVADLVKEGHDVKLVFDAPATKVDSVVQANMVENQITNKADAILLAPMDAEA
                     LVPAIEEVYDAGIPMILIDTLANTKKFHVIYQTDNGAAARLAADELGKLVDGKGEIAI
                     INAQAGSQTTMTRESEFRKEIAEKFPDIKIVGVQYCDGDKQKALNYAEDFMMKNPNLA
                     GFYACNEGSTVGTGNAVDQAGKAGVIKVVGFDFSDEVKDLVSRGVIQATMVQNPWVMG
                     NEGVKAAWKLINGEKVADEEAFVVDTGVTVVTKDNLDEVK"
     gene            complement(2837..3808)
                     /locus_tag="PKV59_04490"
     CDS             complement(2837..3808)
                     /locus_tag="PKV59_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46045.1"
                     /translation="MSQATVEVPTKQKAKMSRQKRQILSTLAALLMLFLFFTLANRNF
                     ISMMNILNILKQATPIIIIAIGQTFVLITAGIDLSIGSVIACSGVVAAIFMRDGMPVW
                     ISMVAGLLFGVLVGVINGSLIVYGKLPAFIATMGTMTTVRGLALFLTQGIPIGSLPKE
                     FTQIGTQRTFNVPNQVYIMAILAIIFGFVLSKTKIGRYTYAMGSNYEATRLSGVNVNL
                     TLIIVYTISGFLAAWAGMIMTANVISAAPTAGDGYELDAVASSVIGGASTLGGEGIIP
                     GTVLGALVIATLRNGLNLSGASPFIQKMIIGIVIIAAVFIDRFKRED"
     gene            complement(3821..>4045)
                     /locus_tag="PKV59_04495"
     CDS             complement(3821..>4045)
                     /locus_tag="PKV59_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016083521.1"
                     /note="with RbsBCD acts to import ribose into the cell;
                     RbsA contains 2 ATP-binding domain; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HQP46046.1"
                     /translation="PMRGIDVGAKVDIYNLMNQLKEQGIGVVFVSSELPEILGMADRI
                     VVMCEGRVTAELDADEANQEIIMQYATQYM"
BASE COUNT         1123 a         1053 c          796 g         1073 t
ORIGIN      
        1 tatgatagat cagaatatgc aggttctgaa tttcatccgt ctgcgattga ttcacagcaa
       61 ttagaatatc ggcatctttt cgcatctttc cgccatccga tccggtcata caaatcacat
      121 aaactcccag ttcctttgcc aataatgccg cataatgcaa attttcggcg tttccactgg
      181 tgctgattga aatcatcaca tctcccggct tggcattcgc gtaaagttcc tgagctacgg
      241 caagattcgg atgctgaaaa tcattttcga tcgcagaccg taaaaccgga ttcaagccta
      301 agaccatcac ccgttgcgct tcttcaagat tttccgctaa aacggaaccc tcttcggact
      361 ttttcaaccg agctgcaaga gccgccggca tgtgcctttt tacaacaaag gctttggaaa
      421 gttctccgct gatatgcagc gcatccgcca tactgccgcc gtttccggca acaaacaggg
      481 ttcctccgca ggaatacgac cgtcggatca actgataggc ttgagcaatc ggttctttga
      541 cattttcaat ttgaggatgc agcctgatcg cttcatctaa aatcaaccgt acagacggat
      601 caatcatttc cgtcatcggc agagaaattc tctttgattc cacgggaaag gtctcgccat
      661 ttgccgtcaa taattgatat aggttcggca aagtgacctt atgatttccg caaatatgga
      721 atccttttcc gcccaacatt accggtcgat tcaccacatt cttgcgcgga cgatagtaat
      781 actgaacttt atcttctccc agaggagcac gataatcgcc cagatcgatc agatcaaaat
      841 ttgcggtggt aaatactttc agcggattcc ccagattccg tgctatggaa agggctttca
      901 gaaaaacttc cggaccgatt accgccgaac cgacattcag aaatactccg ccttccaaat
      961 cacaaaccga tttgcagaaa attttaaaat cctgacccga accccatccg atagccgcaa
     1021 aatccactgc cggatgttga tgaataatat ccgtcccgac agccacatga acggtatagg
     1081 gaattcccaa acgccaagta tggtaaagga cactgacatc gcgatattgg gcaaatcgat
     1141 catcagttcc gatcaactca cccaaagact caccgattcc catccctcgt ttggcaccgt
     1201 tttgaatcgc ggtattcatc agcaaacccg tctcttcggc catcccgaat gtgccgtcct
     1261 taatggaatc cgccacatct tcgcttgtat gtcccaacag cgcaatttca aaatcatgga
     1321 ttgtcgctgc tccgttgctc gcgacatgtt gcacatatcc tttttccaaa agatcgatca
     1381 aaatcggtgc cagaccggat ttgatcacat gcgcaccttg catccaaatt aagggacgac
     1441 ccgcccggcg ggctgccaaa attctttcgg cgacttcttt taaatcggga gaatcaaatt
     1501 ccgcaacagg tgtcgacgga aaaatgaagt catcggtcgc aactcgatta tgacgtgccg
     1561 gaaggggcac tgtacggact ttctttagat caactgcagg ataaggcata tacgtttctc
     1621 cgtgggatat cgaacaaaaa cgatatcata aattttttct ttcttcaaaa gtatcaacta
     1681 tctgtcaaag cgaaatcgat tcttttcatt gagcttttca attttcctct ataaaaagaa
     1741 tttttattcg ctgatattat tggaaaagca tatcattaaa acaaagaacc cggcaaaatt
     1801 gccgggctct ttatgttcga aaaacagatt atttgacttc gtccaaatta tctttggtta
     1861 caacggttac accggtgtcg acaacaaacg cttcttcatc agcgactttc tcaccgttga
     1921 tgagtttcca agcggctttc acaccttcgt tacccatgac ccacggattt tgaaccattg
     1981 tcgcctgaat cacgccacgg gataccagat cctttacttc atcggaaaag tcaaagccta
     2041 ccactttaat gacaccggct tttccggcct gatcaaccgc gttaccggta ccgacagtgg
     2101 agccttcgtt acaagcataa aaaccggcca ggttcggatt cttcatcatg aaatcttccg
     2161 catagttcaa agccttctgc ttgtcaccgt cgcagtattg gacaccgacg atcttaatat
     2221 ccgggaattt ctcggcaatt tccttgcgga attcggattc tcgagtcatg gttgtctggg
     2281 atccggcctg agcattgatg atggcaattt cgccctttcc gtcaaccaat ttgcccagtt
     2341 catccgctgc taagcgagcg gcggcaccgt tatccgtttg gtaaatcaca tggaattttt
     2401 tggtattcgc cagggtatca atcaaaatca tcgggatgcc agcatcatat acttcttcaa
     2461 ttgccggaac caatgcttct gcatccatcg gagcgagcag aatggcatcg gctttgttgg
     2521 tgatttggtt ttctaccata ttagcctgaa ccaccgagtc cactttggtt gcgggagcat
     2581 caaacactaa tttaacgtca tggccttctt ttaccaaatc ggcaaccgca gcttcggcac
     2641 cggccttcac tttcagccag tgctgatcca tggaatccat ggtgatcaat gcgatggtat
     2701 agctcttttc ggcagccatt gcaacactgc aaacagagag taacatcgca agaacaataa
     2761 ttaatgtaac ttttttcatt ctttccctcc taatatattt ttgaaaactg caaaatgcag
     2821 tttatctact actattctaa tcttctctct taaatcggtc tataaaaaca gcggcgataa
     2881 tcacgattcc gataatcatc ttttgaataa aaggagaagc acccgacagg tttaaaccgt
     2941 tccgtaaagt tgcgatcacc aaagctccca aaaccgtccc gggaataatc ccttccccgc
     3001 cgagcgtact ggctccgccg atgacggaag acgctaccgc atccaattca tagccgtcac
     3061 ccgccgtcgg agcagcggaa ataacgttgg cagtcataat cataccggcc caagctgcca
     3121 aaaatccgct gatggtataa acgataatca acgtcagatt gacgttcaca ccggataagc
     3181 gggtggcttc atagttcgaa cccatcgcat aggtatagcg tccgattttg gttttcgaca
     3241 aaacgaaacc aaaaataatc gccaaaatcg ccataatata aacctgattc gggacgttaa
     3301 aagtccgttg cgtcccgatt tgggtaaatt ctttcggtaa gctcccgatc ggaattccct
     3361 gcgtcaggaa taacgccaag ccccggacag tcgtcatcgt acccatggtg gcaataaatg
     3421 ccgggagttt tccatagaca atcaacgaac cgtttattac accgaccaag acaccaaaaa
     3481 gtaagccggc gaccatcgaa atccagaccg gcattccgtc ccgcatgaaa attgccgcaa
     3541 caacacccga acaggcaatc accgaaccga ttgacaaatc aatgccggcg gtgatcaaaa
     3601 caaaagtctg cccgatggca atgataataa tcggagtggc ttgctttaga atattcagga
     3661 tattcatcat ggaaataaaa tttcgattcg ctaatgtaaa gaaaagaaac aacattagca
     3721 aagctgccag ggtactgaga atttgccgct tttgacgtga cattttcgct ttttgcttgg
     3781 ttggaacttc tactgttgct tggctcataa tttacctcat ctacatgtat tgagtagcat
     3841 attgcatgat aatttcttga tttgcttcat cagcgtctaa ttctgcggta accctgcctt
     3901 cacacataac cacaatccga tcagccattc ccaaaatttc aggaagttcg gaagagacaa
     3961 aaaccacgcc aattccctgc tctttcaatt gattcataag gttgtaaata tcaaccttcg
     4021 ccccgacatc aatcccccgc atcgg
//
LOCUS       DAOMIV010000146         4022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23217_length_4022_cov_2.891354, whole genome shotgun sequence.
ACCESSION   DAOMIV010000146 DAOMIV010000000
VERSION     DAOMIV010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23217_length_4022_cov_2.891354"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..1049)
                     /locus_tag="PKV59_04500"
     CDS             complement(129..1049)
                     /locus_tag="PKV59_04500"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQP46047.1"
                     /translation="MNKGLEVFSDSSERLNYNFPDFPLYVRKGTLHQFLRFAAECHWH
                     PDLEFISVLEGFMEYFIDGQIVRINSGSGIFVNSKRLHYGFSADQTDCSYIVVAVHPS
                     LLGENTYPGKAYLKEKFGPLAEDYLILDSTVSWQVEILRSMSELFDEVNQNSPNMLRL
                     LSEAVSICASVGDHIKKNSSQHPDDQSWMIFWKMVDFIHHNYDNKISLKNISEVGSVS
                     KSQCCKLFNKYVGQPPNVYLNAYRILKGCEMLQETNRSICEIALACGFQTASYFSYVF
                     RITKGENPQEYRRRIKNEPKHETELSGMDR"
     gene            1167..2552
                     /locus_tag="PKV59_04505"
     CDS             1167..2552
                     /locus_tag="PKV59_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQP46048.1"
                     /translation="MITELRSENEFDHKFNRDLFTLVLPIALQNLISAAVISADVIIL
                     GMINQSAMAAVSLAGQITFVLTLFYYGFSTGVGIQTAQYWGKKDIGTIGNILNIALIF
                     SESISILFFLLSVSIPEMLMSMFTNDSELIHYGAIFLRTASVSYLAMGLSQMYLSAIK
                     SMEKAGISAAISSFALILTIAFDALTVLVLFPHQPEKAIIGVGISTVFARFVELGLCL
                     LHSKRNNDIRIHLPRPSTIEKTLLKDFIKYTLPVQANYLVWGGALTVTTTIIGHVDSD
                     MVAANSITSAVRNLATVLCSGIAGGGAVLIGKYLGKDDLSSARKAGERLNIYALFFGV
                     VAGMTILISKPLILHLVSLNNTAADYLNGMLIISAYYCVGKSINSTNIGGIFPAGGDP
                     EFGFWTDTIVMWGIVLPLSYLGAFVWHIRPVLLYAIISLDEVIKLPVSIIRFRKYKWV
                     KNITRDPEPVG"
     gene            2621..3220
                     /locus_tag="PKV59_04510"
     CDS             2621..3220
                     /locus_tag="PKV59_04510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HQP46049.1"
                     /translation="MTTMRERINSGKLFSDNCEGLPEDRENAKRKMIALNATGPDDLE
                     TRFRLIKEIFGGKADVWIEPPIYFCYGKNIEIGEGCYINFNCNFVDDGKITIGNHVMF
                     GPAVIVATVSHPINPNFREYMYADPVTIEDNCWIGAGTIICPGVTIGENSVIGAGSVV
                     IKDIPSNSVAVGNPCKVLRTIDENDMKYYGKGREISLDD"
     gene            3299..3508
                     /locus_tag="PKV59_04515"
     CDS             3299..3508
                     /locus_tag="PKV59_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46050.1"
                     /translation="MFPLFAVYLLLWEKEIEIEEERRKNHLAIFVPVEREELKIGRIT
                     DRRGRWKHICQSIRIRIGKIKTISK"
     gene            complement(3688..>4022)
                     /locus_tag="PKV59_04520"
     CDS             complement(3688..>4022)
                     /locus_tag="PKV59_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46051.1"
                     /translation="KKDFDVRFFTPLSDEYRIVVTYHDAERKFTAGVDDNNQGGAKFD
                     YFVDTGQFGNEWCSDKNQTVEEYLIKAYNDPTIKDVFQHSIDLMVQYIHDTFGMTVEK
                     LYALPTGE"
BASE COUNT         1170 a          788 c          889 g         1175 t
ORIGIN      
        1 ctatacgtca ttgctattcc cgatcggaaa actcagaaaa agcaaaagca aatgattttc
       61 aataaagttt tagagtgaca cctctaaaaa acccagaata acaagaattc gtatagaatg
      121 atttcaacct accgatccat gccggacaat tctgtttcat gcttcggttc atttttaatt
      181 cttctgcgat attcttgagg gttttctcct tttgttattc gaaaaacata tgaaaaatag
      241 cttgcggttt gaaatccgca agcgagcgcg atttcgcaaa tcgatcgatt tgtctcttga
      301 agcatttcac aacctttcag gatgcggtaa gcattcagat agacgttggg aggttgcccg
      361 acatacttat tgaacagttt gcagcattgg ctcttgctga cagagccgac ttccgaaata
      421 tttttcagtg agattttgtt atcataattg tgatgaataa aatcaaccat tttccaaaag
      481 atcatccaac tttgatcatc gggatgttgg ctcgagttct ttttaatatg atctccgacc
      541 gatgcgcata tggaaaccgc ttcggaaagc agccgcagca tattcggtga attctggttt
      601 acctcgtcaa ataactcaga cattgaccga aggatttcca cctgccaaga aactgtgcta
      661 tctaaaatca ggtaatcctc ggcaagcggt ccgaactttt ctttcaaata ggcttttccg
      721 ggataggtat tttctcccaa taaagaagga tgaaccgcaa ccacgatata ggaacaatct
      781 gtttgatccg ctgaaaagcc ataatgcaga cgcttgctgt tgacaaagat tccgcttccg
      841 gaatttattc gcacaatttg cccatcgatg aaatactcca taaatccttc aagtaccgag
      901 ataaattcaa gatccggatg ccaatgacat tcggcggcaa accgcaagaa ttgatgtaaa
      961 gttccttttc gcacgtataa aggaaaatcc ggaaaattgt aatttaatcg ttccgatgaa
     1021 tcagaaaaga cttccaatcc tttgttcatg gcttccttta tatctgtttg aatacgattt
     1081 tgatagaatt ctatcctatt ttgggagtaa ttaagcggcg aatctcatac aattttgata
     1141 ggttcatcat tccaaaaagg caaacgatga ttacggaatt aagaagcgag aacgaattcg
     1201 atcataaatt caacagagac ctttttactt tggtcttgcc gatcgccttg caaaatctaa
     1261 tatccgcagc ggtgatctca gccgacgtaa tcattttggg gatgatcaat caatcagcga
     1321 tggcagccgt ttcattggcc ggacaaataa cttttgtatt aactttattt tattatgggt
     1381 tttcaaccgg agtcggtatt caaaccgctc agtactgggg gaaaaaggat atcggaacca
     1441 ttgggaatat ccttaatatt gcacttattt tttctgaaag catttcgatt ttgttctttc
     1501 ttttgtcggt atcgattccc gaaatgctga tgagtatgtt tacgaacgac agcgaattaa
     1561 ttcactacgg agccatattt ttacgcacgg cttctgtttc atatcttgcc atgggcttgt
     1621 ctcaaatgta cctgagcgca atcaagagca tggaaaaggc aggtatcagc gcggcgatca
     1681 gttcgtttgc tctgatactt acgattgcct ttgatgcttt aaccgttttg gtactattcc
     1741 cgcaccaacc cgaaaaagcg atcatcggtg tcggaatctc aacggttttc gcgcgatttg
     1801 tcgagcttgg gctgtgcttg cttcattcaa aaagaaataa cgatatccgg attcacttac
     1861 caagaccaag tacgatcgaa aaaactctgt tgaaggattt tatcaagtac actttaccgg
     1921 tccaagcaaa ttatttggtc tggggcggtg cattgacagt tacgacgacg attatcggtc
     1981 atgtcgactc cgatatggtt gcggcaaatt caatcacatc cgctgtcaga aatcttgcga
     2041 ctgttttatg cagcggaatc gccggcggag gagctgttct gatcggaaaa tatttaggca
     2101 aggatgactt gtcttcggca agaaaagccg gggaaagact gaatatctac gcacttttct
     2161 ttggggtggt ggccggaatg acaatcctga tatcaaaacc gctgatttta catcttgtaa
     2221 gtctgaacaa taccgccgcc gactatctca atgggatgtt gatcatcagc gcctattact
     2281 gtgtcggaaa atcaatcaac tcgacgaaca tcggtggcat ctttccggcc ggcggagatc
     2341 ccgaattcgg tttttggact gatacgatcg tgatgtgggg gatcgttttg ccgttaagtt
     2401 atttgggcgc ttttgtttgg catattcgtc cggttctgtt atatgcaatt atcagcttag
     2461 atgaggtaat caagttgccg gtatcgatca ttcggttccg aaaatataaa tgggtcaaaa
     2521 atattacccg tgatcctgaa cctgtgggat agaagagtaa tgacgagagg ctgtttttgc
     2581 aaaccggtaa taaaaaattt agtgacaagg agtgagaaaa atgacgacga tgagggaaag
     2641 aataaattcg ggaaagttat tttcagataa ttgtgaagga ttgcccgaag atcgtgaaaa
     2701 tgccaaaaga aaaatgattg cactcaatgc gaccggtccg gatgatttgg aaacgcgatt
     2761 tcgtctcatc aaagagatct tcggcgggaa agccgatgtt tggatcgaac cgccgattta
     2821 tttttgttac ggaaaaaata ttgaaatagg tgaaggctgc tatatcaact tcaactgtaa
     2881 ttttgtggat gacggaaaga tcacaatcgg gaatcatgtc atgtttggac cggcggtcat
     2941 tgtcgcaaca gtcagccatc ccatcaatcc gaattttcgc gaatacatgt atgctgatcc
     3001 cgtaacgatt gaagataact gttggatcgg ggcgggaacg atcatttgcc cgggcgttac
     3061 gatcggagaa aacagtgtaa tcggcgcggg gagtgttgtg ataaaggata ttccttccaa
     3121 ttctgtagcg gtcgggaatc cttgtaaggt actccggacg atcgatgaaa atgatatgaa
     3181 gtactacgga aaaggacgcg aaatctcatt ggatgattga gatgatgaga ttgaatggtg
     3241 atgattccgc cattcacgag ccgatgaagc acattctgta agttatcgga ggagttctat
     3301 gtttccgctt tttgccgttt atcttcttct atgggaaaaa gagattgaaa tagaggaaga
     3361 gagaagaaaa aatcatctgg cgatttttgt ccccgtagaa cgagaggagc taaaaatcgg
     3421 acggataacc gatcggcgcg gaagatggaa gcatatctgt caatcgataa gaatcagaat
     3481 cgggaaaata aaaactattt ccaaataatg tcgccgtata tttcatttta ggagtcggat
     3541 ggcagtttcg aggaaatctt taatgatttt ccgtaaaaag aaacgttcgg tttaagtgac
     3601 agaggaaatt cttatctttt ttgattcaaa aggattgatc gataactgta cattttattt
     3661 gagattaaaa ccggagtcct ttacacttta ttcacccgta ggtaatgcgt atagcttctc
     3721 aaccgtcatc ccaaaagtat catgaatgta ttggaccatt aggtctatgg aatgctgaaa
     3781 gacatccttg atcgtggggt cattataagc tttgataaga tattcttcta ccgtttggtt
     3841 cttatcgctg caccattcgt tgccgaactg gcctgtatcc acgaaataat caaatttcgc
     3901 gccaccctga ttattgtcat caactccggc agtgaatttt ctttccgcat catgataggt
     3961 gacaacgatg cggtattcat cacttaaagg agtaaaaaag cgaacgtcaa aatccttctt
     4021 tc
//
LOCUS       DAOMIV010000147         4018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23250_length_4018_cov_3.588695, whole genome shotgun sequence.
ACCESSION   DAOMIV010000147 DAOMIV010000000
VERSION     DAOMIV010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23250_length_4018_cov_3.588695"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKV59_04525"
     CDS             <1..453
                     /locus_tag="PKV59_04525"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQP46052.1"
                     /translation="WDSGKSIEQVRDWLEENKLKMNHWFTVDDLHFLERGGRVSASSA
                     WFGSALNIKPVMNMDDAGHLIPREKIMGRNKAIKRLYEKMKEMAVEPQNQRIFISHGD
                     CMEDVEKLEKLIHDNLGVNDFTVNYVGNVIGSHTGPGVLALFFLGNHR"
     gene            complement(606..3344)
                     /gene="alaS"
                     /locus_tag="PKV59_04530"
     CDS             complement(606..3344)
                     /gene="alaS"
                     /locus_tag="PKV59_04530"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HQP46053.1"
                     /translation="MEKKLSGNEIRQQFIDYFVERGHTAVRSASLVPAEDQTLLFTNA
                     GMVQFKDVFLGTDVRPYKRACDSQKCMRVSGKHNDLDDVGRDDFHHTFFEMLGNWSFG
                     DYYKKEAITWAWDLLTNVWGLDKDILWATCFRDEKDEIPTDEEAFEIWNTQPGMNKDH
                     VLYFGRKENFWEMADTGPCGPCSEIHIDLGESHCNKKGIPGHICHVNGDCGRFLELWN
                     LVFIQYNRLNETTLNPLTQHFVDTGMGFERIVSILQKTDSNYKTDLFLPMIREIQKLT
                     KVSDDEMYSNFTPYRVIADHARASAFLIADGVVPGNIGRNYVCRMIIRRAVRFGYQLG
                     LKEPFMSKVAEKVIENYGDAYPELIRMKESILHSVSWEEERFNKTLENGINQLNEIIE
                     EMKRSGQKIIDGETCFNLYSTHGLPLEITYDIVREYGLDVDREGFKKASEAHRIASGA
                     GKIFGKMGGEDAEQYGMHLSFLKESKLLGEEGVICDPYQTKDVEAKLIMALVDGQRVE
                     KIEKGQFAELIVSQTNFYMEMGGQVGDNGWIRSEDGTFEFKVEGIRRPVAGLTIHYGK
                     AESGSISVGETVKVVVDKQRRKDIMRNHTATHLLHAALREVLGEEARQAGSLVAADHL
                     RFDFNSSRALTNEELSKVETIVNEKILEGIPVTTTIEKLDDAINEGVTAIFNEKYGEI
                     VRVVRVEDDDELFSAELCGGTHVSNTGEIGLFMIMSEGSVSTGIRRIEAITGRTANMT
                     ARKNTQIVKNLASILNASTEEIFEKVENLQALSNKYQKEIVQLQTQIALKDFKKFWEN
                     AQKIKDANVLTAMISDSTIESLRNMADQFKAENPNAVVVLGTNTDDGQVQFIAAVSDS
                     LIQRGINAGEIIRNISALTGGKGGGRPNMAQGGGKDITKAQEALDAAVKMVREKLR"
     gene            3650..4018
                     /gene="thpR"
                     /locus_tag="PKV59_04535"
                     /pseudo
     CDS             3650..4018
                     /gene="thpR"
                     /locus_tag="PKV59_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
BASE COUNT         1154 a          889 c          707 g         1268 t
ORIGIN      
        1 tgggattcgg gaaaaagtat tgaacaagta agggattggc tcgaagaaaa taaactcaaa
       61 atgaatcatt ggtttaccgt tgatgatctt cattttttgg agcgcggagg tagagtatca
      121 gccagctccg cttggtttgg cagtgcatta aatattaaac cggttatgaa catggatgat
      181 gccgggcatt tgattcccag agaaaagatt atgggaagaa acaaagcgat caaacgatta
      241 tatgaaaaaa tgaaagaaat ggcggtagaa cctcagaacc aacggatttt catcagccat
      301 ggtgattgta tggaagatgt ggaaaaactt gagaaattaa ttcatgataa tttgggggtc
      361 aatgatttta ccgttaatta tgtcggcaat gttatcggtt cgcatacagg acccggtgtc
      421 ttagctttat tctttttggg aaatcatcga taagtaaaaa aaataaacaa attaagatta
      481 acaagaatga gggcaccata aaagtttgat agcgccctca tttttatatt aaatgtttta
      541 ttattcggtt gaataaagtt tcttcggtat ccttgccgat taacaatttt tcttcacaaa
      601 ttaatttatc gaagcttttc ccgaaccatt tttacagcgg catccaacgc ttcctgcgct
      661 ttggtgatat ctttcccgcc tccctgtgcc atattgggac gtcctccacc tttacccccg
      721 gtaagggctg aaatatttcg aatgatctcc ccggcattga ttccgcgttg aatcagtgaa
      781 tcagaaacag cagcaataaa ttgaacttgt ccgtcatctg tattcgtgcc taagacgaca
      841 acagcattgg gattttctgc cttaaattga tcagccatat ttctcagcga ttcaattgtt
      901 gaatctgaaa tcattgcggt taatacattc gcatctttaa ttttttgtgc gttttcccaa
      961 aacttcttaa aatcttttaa tgcaatttgt gtttgaagtt gaacgatttc tttttgatac
     1021 ttattcgaaa gtgcttgcaa attttccact ttttcgaata tttcttctgt ggaggcattt
     1081 aagattgatg ccaaattctt cactatttgt gtattctttc gtgcagtcat atttgcagtg
     1141 cgtccggtaa tagcttcaat acgtcggatg cctgtcgaaa cactgccttc gctcattatc
     1201 atgaacaatc cgatttcacc ggtattgctt acatgagtgc caccgcataa ctcggcagaa
     1261 aaaagttcat catcatcttc cactctgact acgcgaacta tttcaccgta cttttcattg
     1321 aatattgcag tcacgccttc atttatagca tcatctaatt tttcgatagt ggttgttacc
     1381 ggaatacctt ccaaaatctt ttcgttaaca attgtctcga ctttgcttaa ttcttcattt
     1441 gtcagagctc tacttgaatt gaagtcaaat cgtaaatgat cggcagcaac caaagatcct
     1501 gcttgccttg cttcttcacc gagtacttct ctcagagcag catgtaatag atgagttgcg
     1561 gtatggtttc gcatgatatc cttgcgacgc tgcttatcga caaccacctt gacagtctct
     1621 cctaccgaaa tacttcccga ttctgctttc ccgtaatgaa tggtaagtcc ggcaaccggc
     1681 ctacggattc cttccacctt aaactcaaaa gttccgtctt ctgaacgaat ccacccgttg
     1741 tctcctactt gcccgcccat ctccatataa aaattcgtct gcgaaacaat cagttcggca
     1801 aattgacctt tttctatttt ttcaacacgc tgtccgtcta ccagagccat gatcaatttg
     1861 gcttctacat ccttggtctg ataaggatcg caaattacgc cttcttcacc caacaacttt
     1921 gactctttca aaaaggacag atgcatccca tattgttccg catcctctcc ccccattttt
     1981 ccaaaaatct tcccggcacc ggaagcaatg cgatgagctt cacttgcttt tttaaatcct
     2041 tcacgatcaa catccaaacc gtattcacgg acgatatcat aggtaatttc aagaggaaga
     2101 ccgtgcgttg aataaagatt gaaacaagtt tctccatcga ttattttttg accgctcctc
     2161 ttcatttcct caataatttc gttgagttgg ttgattccgt tttcaagtgt tttattaaat
     2221 ctctcttcct cccaggatac cgaatgaaga atggattctt tcattctaat taattccgga
     2281 taagcatctc cataattctc aatcaccttt tccgcaactt tactcatgaa cggttctttc
     2341 aagccgagtt ggtaaccaaa tcgaacagct ctgcgaataa tcatccggca aacatagttt
     2401 ctgccaatat tcccgggaac aacaccgtcc gcaatcagaa aagcagaagc gcgagcgtga
     2461 tcagcgatca ctcgataagg cgtaaaattc gaatacatct cgtcatcgct tactttggtt
     2521 aatttttgaa tttcacgaat catcggcaaa aatagatccg tcttataatt tgagtctgtt
     2581 ttttgcagaa tcgatacaat tctttcaaat cccatacccg tatcaacgaa atgctgagtt
     2641 agtggattta atgtagtttc attcaggcgg ttatattgga taaacaccaa attccataat
     2701 tccaagaaac gaccgcaatc tccgtttaca tgacaaatat gacccgggat gcctttttta
     2761 ttgcagtggc tttcacccaa atcgatgtga atctcactgc aaggcccaca tggtcccgtg
     2821 tcagccattt cccagaaatt ttccttccgt ccgaaataca ggacgtggtc cttattcatt
     2881 ccgggctgag tattccaaat ttcaaaggct tcttcatcgg tgggaatctc atctttttca
     2941 tctctgaaac atgtagccca aagtatatct ttgtctaatc cccatacatt ggtcaaaaga
     3001 tcccatgccc atgtaatcgc ttcttttttg taataatctc cgaaagacca atttcccagc
     3061 atttcaaaaa aagtgtgatg aaaatcatca cgtccgacat catccaaatc gttgtgtttt
     3121 cccgagactc gcatacactt ttgactgtca caagcacgct tatagggtct tacgtccgtt
     3181 ccgagaaaaa catctttaaa ttgaaccatc cccgcattcg taaatagaag agtctgatcc
     3241 tcagccggaa caagcgatgc agaccgtacc gctgtatgac ctctttcaac aaagtaatca
     3301 ataaattgtt gccgaatttc attcccgcta agcttttttt ccataagaga ctctccggta
     3361 atcctgtttg ttattcatct tttttgctga attattataa accagcagat atcgatagtg
     3421 aataaaaaga gcgattaaac agacaatttt ttcggttgaa tttctccctt ccttactctt
     3481 tattcttgca atattcattt aatatcagta tggaaaaaag gtataatttg cattcagttt
     3541 agttcgaggc tgtttataaa atcatgatga aacaaaatgc gactcttcgt ctttttattg
     3601 ctatagagct tcctgagatg attcgaaaac cactctcaga cagcttgaag gactctgtca
     3661 ttatcaaaaa aaagaagcac gattaacctc caaagagaac cttcatatca cattgaagtt
     3721 tttaggagat gtacaacaag acaaaagggg tgatcttcag acagcaattg cgaattcaat
     3781 cggcggaatc ggtcctttcg aactcaaaat tcattccgga ggcgttttcc cgacgataaa
     3841 aaagccgcgt attctttggg ccggatttga tatttcgaaa gaattatccg ttttggtgaa
     3901 caaccttgag aatgagtttg aaaaaatcgg atattcgaaa gatttaaaac catttcaacc
     3961 gcacataacg ttggctcgaa ttccggatta tgtatctaca atgattcctg tcgaaaaa
//
LOCUS       DAOMIV010000148         4005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23349_length_4005_cov_4.370886, whole genome shotgun sequence.
ACCESSION   DAOMIV010000148 DAOMIV010000000
VERSION     DAOMIV010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23349_length_4005_cov_4.370886"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="PKV59_04540"
     CDS             complement(<1..759)
                     /locus_tag="PKV59_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQP46054.1"
                     /translation="MITSIQNSKIQWVKKLLTEKKERQESGWYVMEGIRLSEEALQKE
                     IPIQFALYSSPLTNRTENFIKALQKAGIEAEEIPPQLMRSVSDTTTPQGILIVVKAQP
                     LPIQPDPSLVLILDGIHDPGNLGTIFRTAAAAAVDLILLAPGCADPYSPKVVRSGMGA
                     HLKIPFSQSDWREIDDFFQVHPHIFPLASDMKGGKSLWEEDLRRPAAIIIGSEAEGIC
                     KEAEQLARGKIFIPMSPGTESMNAAVAAGILMFEA"
     gene            954..1256
                     /locus_tag="PKV59_04545"
     CDS             954..1256
                     /locus_tag="PKV59_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQP46055.1"
                     /translation="MKIKPEDARKMLESENPPALIDVREPDEFASGHIPNAVNVPLGD
                     VVNGVIRLGIPKDQPVMVYCRTGRRSSEAASKLQGAGYQKIYDLGGITDWPYEIVK"
     gene            complement(1273..3360)
                     /locus_tag="PKV59_04550"
     CDS             complement(1273..3360)
                     /locus_tag="PKV59_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46056.1"
                     /translation="MKQKKSEKIKQITLSLIMAVISVQCALSIWRRYQEFLSKGSIVP
                     SFLLICTFWIVGILFIYAILWRPELLKMFLFLKKLPDPIRRMGSFFICLLPIFLYTFH
                     RWSEPFGNPWIRIFLYTVIILAAAWLLTKEKGISGGALIASMLAFSVSFLLINQFRDL
                     TDYPFSVGWSEGNRFWDYSVLFGRRLYNWPQDKPIPAYIDLGRQSLWGAIFLLPKVTI
                     RMMRAWNDFLFTIPYALLGWALLNYRSVTQKKYLLAGSLWAMIFLNQGPIYTPLVLAA
                     ILVALGHKLPLWLGCLFTFLAGAYAVMSRSTWIVAPAIYGGMLTLADTYDKKILSQKR
                     RWFQAILIGLFGIAGAFSYLNQYSIRAFFSESSVTEKSTVTVPLNYGEEYQSVDTSPK
                     ETEASESVPAMLTPAWFQYYLSRQPLLWNRLWPNETYKLGIVPGLLMAVLPLVLLLIF
                     WDHEKHWNLSGMQRLVLGCILFAFLCVGLIVSVKIGGGSNLHNLDMFLIALLIIAAMA
                     WNAGMGEWLIDKVHSGHWINWIILAAIILPVLPAMLTVEPKKYPDPSTTGEALAKVQQ
                     VIAQHAQEEILFMDQRQLLTFGYVPKIPLIADYEKKWMMDEAMADNAEWFKPYIHDLK
                     NQRFAVIISEPLQIKFQGANRNFSEENDLFVKWVSIPTLCYYEPLETFEEQGVQILVP
                     RKSVLVQEGVTCP"
     gene            complement(3357..>4005)
                     /locus_tag="PKV59_04555"
     CDS             complement(3357..>4005)
                     /locus_tag="PKV59_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQP46057.1"
                     /translation="YSIIEVEDQALKDILAERCDHQPFIATAPWLLLFVADYQRWFDY
                     YKVSNAPALCAEFGRVPRTPQEGDFLLACMDTLIAAQNAVIAAEALGLGSCYIGDIVE
                     HYEQIRDLFRLPRYAAPIVLLCFGYPTEDQKNRKPVERFDKKYIVHKNQYPSFSDQDL
                     LNMFPKEDIIPNNPLGRLPQGQRNYIRKFTADFSIEMSRSVREMLKNWQQTNPDS"
BASE COUNT         1152 a          902 c          900 g         1051 t
ORIGIN      
        1 cgcttcaaac atcagaatac cggcagccac tgctgcattc atcgattccg tcccaggact
       61 catcgggata aaaattttgc cccttgccaa ctgttccgct tctttacaga ttccttccgc
      121 ttcggatccg atgatgattg cagctggccg gcggaggtct tcttcccaaa gagattttcc
      181 tcccttcata tccgatgcaa gcggaaaaat gtgcggatga acttggaaaa aatcatcgat
      241 ttcccgccaa tcggattgag aaaaaggaat tttcaaatga gctcccattc cggagcgaac
      301 cacttttggc gagtatggat cggcgcatcc tggggcgaga aggattaaat cgaccgccgc
      361 tgcggcagcc gtccgaaaaa tcgttcccaa attgccgggg tcgtgtattc cgtccaaaat
      421 gagcacaaga ctcggatcgg gctgaatcgg caacggttgc gctttgacaa cgattaatat
      481 gccttgggga gtcgtcgtat ccgatacgga tcgcatcagt tgcggaggaa tttcttccgc
      541 ttcaattccg gctttttgca gtgctttgat gaaattttct gtacgatttg tcaagggcga
      601 tgaatacaga gcaaattgga tgggaatttc tttttgcaga gcttcttcgg acaatcggat
      661 tccttccatg acataccagc ccgattcttg gcgttctttt ttttctgtta aaagtttttt
      721 tacccattgg attttcgaat tctgtatcga tgtaatcata tatattcaac ccaaatcatt
      781 tctgatgtta gaatttgcca tcattatgca tcaatcctat ttccccaaag ggcgccatga
      841 aaagactttt tctatttttt attgtaatct atatctcaat ctttctcggg ggttgttcgg
      901 aacgaggcac gattgaaggt caaacaaaac cggaagaaga ggtaacagtt gttatgaaaa
      961 tcaaaccaga agatgcaaga aaaatgcttg aaagtgagaa tccaccggct ctaatcgatg
     1021 ttcgcgaacc ggatgaattt gcgagtgggc acataccgaa tgctgttaat gtcccgttgg
     1081 gagatgtggt aaatggggtc attcgattgg gaatcccgaa agatcaaccg gttatggttt
     1141 attgccgcac gggaaggcgg agttcggaag ccgcttcaaa attacagggt gcgggatatc
     1201 agaagattta tgatctggga ggaatcaccg attggccgta tgaaatcgta aaataagcat
     1261 ggtgtcttct gtttatggac aggttacccc ctcctgaacc aatacacttt tacgcggcac
     1321 aaggatttga acaccctgtt cttcgaatgt ctccaacggc tcataatagc atagggtcgg
     1381 gatagaaacc catttaacga acaggtcatt ttcttcgctg aagtttcggt ttgccccctg
     1441 aaatttaatt tggagcggtt cgctgatgat aaccgcgaaa cgttgatttt ttaaatcgtg
     1501 gatatatggt ttgaaccact cggcattatc cgccattgct tcatccatca tccatttttt
     1561 ctcataatcg gcgattaaag gaattttggg aacatatccg aaagtcagca actggcgctg
     1621 atccatgaaa agtatctctt cctgtgcatg ctgggcaata acctgctgca ctttggccaa
     1681 tgcctcgccg gttgtactcg gatccggata ttttttcggt tcaacggtta acatggccgg
     1741 cagaaccgga aggatgattg ccgccagaat aatccagttg atccaatgtc cggaatgaac
     1801 cttatcgatt aaccattcac ccattccggc attccatgcc atcgctgcga tgatgaggag
     1861 agcaatgaga aacatgtcta aattatgtaa attggacccg ccgccgattt tgaccgatac
     1921 gatcagcccc acacataaga aagcgaacag gatacagcct aaaaccaagc gttgcattcc
     1981 gctgaggttc caatgttttt catgatccca aaatatcaat aaaaggacca aaggcaggac
     2041 tgccatcaag agtcccggta cgattccgag cttgtatgtt tcattcggcc agagccgatt
     2101 ccaaagcaaa ggttggcggg ataaataata ttgaaaccat gccggggtaa gcatcgccgg
     2161 aacgctttcg gaagcttccg tctctttcgg agatgtatcg accgattgat attcctcccc
     2221 ataattcaac ggtaccgtaa cggttgattt ttcggttacg gatgattcgg aaaaaaacgc
     2281 tctgatggag tattgattca aataagagaa agcgcccgct atgccgaaca agccgatcag
     2341 aattgcctga aaccatcttc ttttttgact gagtattttc ttatcataag tatcggcaag
     2401 ggttaacatt ccgccgtaaa ttgccggagc aacgatccaa gtggagcggg acattaccgc
     2461 ataagccccg gcgagaaatg tgaacaagca tcccaaccaa agaggaagtt tatgtcctaa
     2521 tgcgaccaat attgcggcaa gaaccaaagg tgtatagatc ggtccctgat tcaaaaaaat
     2581 cattgcccaa agcgagcctg ccagcagata ttttttttga gtgacggacc ggtaattcaa
     2641 taatgcccaa cccagcaacg catacggaat ggtaaaaaga aaatcattcc aggcacgcat
     2701 cattcgaatt gtcactttgg gcagcaaaaa gatagccccc cataagcttt gacgcccgag
     2761 atcgatataa gcaggaatcg gtttatcctg cggccaatta tataagcgtc gtccgaaaag
     2821 aacggaataa tcccaaaagc gatttccttc cgaccaaccg acgctgaacg gataatcggt
     2881 aagatcacga aactgattga tcaataaaaa tgaaacactg aaagccaaca ttgaagcgat
     2941 caatgccccg ccggatattc ccttttcttt tgtaaggagc catgcggcgg caagaatgat
     3001 caccgtatac agaaatatgc ggatccaagg atttccaaac ggttcactcc aacgatgaaa
     3061 tgtatagaga aaaatgggaa gcagacaaat gaaaaatgac cccattcgtc tgatcggatc
     3121 agggagcttt tttaaaaaca agaacatttt cagcagctcc ggtcgccata agattgcgta
     3181 aataaaaaga attcccacaa tccaaaaagt acaaatcagc aaaaacgaag gaacgatgct
     3241 gcctttggac aagaattcct gatatcgtct ccagatgctt aacgcacatt ggacggaaat
     3301 gacagccatt atcaggctca gagtgatttg tttgattttt tcggattttt tttgtttcat
     3361 gaatcggggt ttgtctgttg ccaatttttc aacatctcgc ggaccgaacg ggacatttcg
     3421 attgaaaaat cagcggtaaa tttgcgaata taattccgtt gaccctgtgg taaacgcccc
     3481 aaggggttat tcgggataat atcttctttc ggaaacatgt tcaacaggtc ttgatcggaa
     3541 aaagacggat attgattttt atgaacaata tattttttat caaaccgctc aaccggtttt
     3601 cggttttttt ggtcttccgt cgggtatccg aagcacaata agacgatcgg agcggcatat
     3661 cgtggcaagc ggaaaagatc ccgaatttgt tcataatgtt cgacaatatc accgatataa
     3721 cacgaaccca agccgagtgc ctccgctgcg atgactgcat tttgcgcagc gatcagggtg
     3781 tccatacaag ccagcaaaaa gtcaccttct tgcggagttc ggggaactct gccgaattcg
     3841 gcacacagcg ccggagcgtt tgaaacttta taataatcaa accagcgttg atagtccgca
     3901 acgaataaaa gcagccacgg tgcagttgcg ataaacggtt gatgatcgca cctctccgca
     3961 aggatatctt tcaaggcttg gtcctcaact tcgatgatag aataa
//
LOCUS       DAOMIV010000149         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23570_length_3977_cov_5.067313, whole genome shotgun sequence.
ACCESSION   DAOMIV010000149 DAOMIV010000000
VERSION     DAOMIV010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23570_length_3977_cov_5.067313"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..2579)
                     /locus_tag="PKV59_04560"
     CDS             complement(72..2579)
                     /locus_tag="PKV59_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQP46058.1"
                     /translation="MAGIDRFTQRARRVLSLAHQEADKNNETIIGTEHLLIGLLLVEG
                     STAGKVLQDLDVSADRVREALSKMEPKTEILGIPEAGQEEALSAEVQDILKRAVIQAS
                     KNGQVYVSTEHLLLSLIETENSRAVAVLKRLGISPEQIRKQLKRVLEEGNSAPASNST
                     EEVKPKPVKKQRTDKTTSNSPLIDQLATDLTAKAAAGKLDPVVGRETEIARVIQVLAR
                     RTKNNPALVGEPGVGKTAIVEGLAQAIVEGNVPAQLLNKRVLQLDVGSMVAGTMYRGQ
                     FEDRMKRVIEELKNTKAILFIDEIHMLVGAGSSGSAVDAANILKPALSRGEVQVVGAT
                     TSDEYRKNIESDAALARRFQQILVNEPTPEETIQILKGLRPRYEEHHHLNISDEALEA
                     AVKLSVRYVSERFLPDKAIDLIDEASSRVRMYKNPSTALTDDYMAELRKTRKDGLLAI
                     EQGDADLAASLYERGLVLEDRIEEASKIWNREEAPVVTAEDIAEIVSMWTGIPVMQMA
                     EEESERLLRMEEELGKMIIGQNEAISEISHAIRRSRSGLKDSNRPIGSFLFLGPTGVG
                     KTELTKALAKYLFGSEEAMIQLDMSEFMERHTVSRLTGAPPGYVGYEEAGQLTEAIRR
                     HPYSIVVFDEIDKANPEVLNMLLQIMEEGHLSDARGKKVDFRNTIIVMTSNIGADVIR
                     RQNNFGFNLIEGKDEEEQESYEEMRKKLMDQLKKSFRPEFLNRLDNIVVFRMLNLEDM
                     QQITEIEIGKVNQRLADKQITLTVSESALEKLTRDGFDPEMGARPLRRVIQQQIEDPL
                     SDALLAREFSNGDQVLITLDDKNEIAFRKVEPQREEL"
     gene            complement(2749..3768)
                     /locus_tag="PKV59_04565"
     CDS             complement(2749..3768)
                     /locus_tag="PKV59_04565"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HQP46059.1"
                     /translation="MPTHEPLILEYRNEVIDNVHMGYVCIVDENSKVLFHIGDPSEYV
                     YYRSASKPIQALPVIMRDLDKKYGITEEESVIFSASHSGEPFHIAILESIFAKAGLKE
                     EMLIMKPTYPGYFPAKLELLKKDLPPRKFYHNCAGKHAGAMLLQRELGGKVEDYWRPD
                     SPAQKEIKRAVMALSETNEDNTKVGIDGCGVPVFAVGIKNIAAGYKNLACIDTIRDEK
                     LREAAARYLPRINRYPHMIRGTGYLCSLMNRDSNLISKDGAAGVYGCGMRKERLGISF
                     KMSSGEDTVWPLILKSIFTQIGGQDPQTLEMLDKLRPDSVFNDNDLVVGHYESLICLD
                     RKPIR"
BASE COUNT          969 a         1043 c          760 g         1205 t
ORIGIN      
        1 atctcggtta ggggtgcggt gctttttagc agccatctgc aaaattttat ctcatttgca
       61 gcgttttcag attacaattc ttctctttgc ggttcaacct tccgaaaagc aatttcgttt
      121 ttgtcatcca aagtaatcaa tacttggtct ccgtttgaaa attctcttgc caataaggca
      181 tcggataacg gatcttcgat ttgttgctga atcactctgc gcagcggacg ggcacccatt
      241 tcgggatcga aaccatcccg ggttaatttt tccaaggcgc tttcgctgac agtcaaagtg
      301 atctgtttat cggctaatct ttggttcact ttgccgattt cgatctccgt aatctgctgc
      361 atatcttcca aattcaacat tcggaagaca acaatgttgt ccagacggtt gaggaattcg
      421 gggcggaaag actttttcag ctgatccatc agctttttcc gcatctcttc atagctttcc
      481 tgctcttcct catcttttcc ttcgatcaga ttgaagccga aattattctg gcgccggata
      541 acatccgcac cgatgttgga tgtcatgaca atgatcgtat tccggaaatc gaccttcttc
      601 ccgcgagcat ccgaaaggtg tccctcttcc atgatttgca acagcatatt caacacttcc
      661 ggattcgctt tgtcgatttc gtcaaaaacc acaatggaat atggatgtct gcgaatagct
      721 tccgtaagtt gtccggcttc ttcataaccg acatatcccg gaggcgctcc tgtcaaccgg
      781 ctaacggtat gcctttccat aaattcggac atatccaatt ggatcatcgc ttcttcgctg
      841 ccgaaaagat attttgccag tgctttcgtc agttccgttt tgccgacacc cgtcggtccc
      901 aggaacaaga acgaaccgat cggtcgattt gaatccttca gaccggagcg tgatcgtcgt
      961 atcgcatgtg aaatttccga tatcgcttcg ttttgcccga taatcatctt accgagctct
     1021 tcttccatac gcagcaagcg ttcggattct tcttccgcca tttgcataac cggaatccct
     1081 gtccacatgg aaacaatttc cgcgatatct tcggcggtta caaccggtgc ctcttcccga
     1141 ttccagatct tactggcttc ttcgatccga tcttccaata caagacctct ttcatagagc
     1201 gacgctgcca aatcggcatc gccttgttcg atcgccagca atccgtcctt tctggttttg
     1261 cgcagctcag ccatataatc gtcggtgagt gccgtggaag gatttttata catgcggact
     1321 cttgaagaag cttcatcaat caaatcaatt gctttatccg gtaaaaagcg ttcggaaaca
     1381 taacgtacgg ataatttgac cgccgcttcc aaagcttcgt ccgaaatatt caaatgatga
     1441 tgctcctcat accgcgggcg taatcctttc aagatttgaa tcgtttcttc gggtgtcggt
     1501 tcattgacca ggatttgttg gaaacgcctc gccaacgccg catcactttc gatgtttttc
     1561 cgatattcgt cggatgttgt cgcaccgacc acctgaactt ctccgcggga taacgccggt
     1621 ttcaatatat tcgcagcgtc gaccgcagaa ccggaagatc cggctccgac caacatatga
     1681 atttcatcga taaacaatat cgctttggta ttttttaatt cttcaattac ccgcttcatt
     1741 cgatcttcaa attgcccgcg atacattgtg ccggcaacca tagaaccgac atccaactgc
     1801 aatacccgct tgttcagtaa ttgcgccgga acgtttcctt ccacgatcgc ctgggctaaa
     1861 ccctccacaa tcgcggtttt accgacaccc ggctctccga ccaatgccgg gttatttttc
     1921 gttcgcctgg caaggacttg gataacccga gcaatttcgg tttctctgcc gacaaccgga
     1981 tccagtttcc ccgccgcggc ttttgcggtt aaatcggttg ccaactgatc gattaacggt
     2041 gaatttgaag tcgttttatc cgtcctttgt ttctttaccg gtttcggttt tacttcctct
     2101 gtcgaattgg atgccggtgc agaattccct tcttccaaca cccttttcag ctgcttacga
     2161 atctgttcgg gactgatgcc gagccttttt aacaccgcaa ccgcccgtga attttcggtc
     2221 tcaattaaac tgagtaataa atgctccgta ctgacataaa cctgtccatt tttagaggct
     2281 tgaattaccg cacgctttaa aatatcctga acttcggcgg ataatgcctc ttcctgtccc
     2341 gcttcaggaa ttccaagtat ttcagtcttc ggctccatct tggataaggc ttcccgtacc
     2401 cgatctgcgc ttacatccaa atcctgcaaa actttcccgg cggtagagcc ttcaaccaat
     2461 aaaagcccta tcagaagatg ttccgttccg attatcgttt cgttattttt atcagcttct
     2521 tgatgagcaa gacttaatac ccgcctcgct ctttgtgtaa atcgatctat tcctgccatt
     2581 ttttatctca ttttcttact ataaccgata taactcatca gttcttcgtt attaatttta
     2641 acagaataat tttcaaaact atcctgccga attgtaccag agttcatatc taatatacgt
     2701 ttgaaagggc agtttcgttt gttagaaatc tattaatttc tccgaaattt atcgaatagg
     2761 cttccgatcg agacaaatca gggattcata atgtcccacg accaaatcat tatcattgaa
     2821 cacactatcc gggcgcaatt tatccagcat ttccaatgtt tgaggatcct gtccgccaat
     2881 ttgagtgaag atgcttttta atatcaacgg ccaaaccgta tcttccccgc tgctcatttt
     2941 gaatgatatc cccagccgct ctttccgcat accgcatccg taaacgcctg cggctccatc
     3001 ctttgaaata agattggaat cccgattcat caggctgcaa agatagcccg tcccgcgaat
     3061 catatgcgga taacgattga tacgcggcaa atatctggca gccgcttccc gcaatttttc
     3121 atcccgaatc gtatcgatac aagctaaatt tttatagccg gcagcgatat tcttaattcc
     3181 gaccgcaaag accggcacac cgcaaccgtc aatgccgacc tttgtattgt cttcattcgt
     3241 ttcactcagc gccataaccg ctctttttat ttccttttga gcgggagaat ccggccgcca
     3301 ataatcctcc acctttcccc ctaattctcg ctgcagaagc attgctccgg catgttttcc
     3361 cgcacaatta tgataaaact tgcgcggcgg taaatctttc ttcaataatt ccaacttcgc
     3421 cggaaaataa cccgggtaag ttggcttcat tatcaacatt tcttccttca gtccggcttt
     3481 ggcaaaaatc gattccaata tggcgatatg gaaaggctcg ccagaatgcg atgccgaaaa
     3541 aattacactt tcttcctctg taataccgta ttttttatct aaatcacgca tgatcaccgg
     3601 taaagcttgg atcggcttgg atgccgagcg gtaatacaca tattcggacg gatcaccgat
     3661 atgaaacaaa acttttgaat tttcatcaac gatacagaca tatcccatat ggacattatc
     3721 gataacttca tttcgatatt ccaaaatcaa aggttcatga gtgggcattt taatctcctt
     3781 atacagattt tgatccggct gagctgaatc aaatcataat tcgcatctct atgccaatta
     3841 tggcacgagt atattttttg tgtgccggtt ctcccgccaa acaagtcctg ccaacaggat
     3901 taccgtaaga atcgttatgg ccatcccgat caataaaccg ttggtatagg aaggatcgtg
     3961 gtgatttgcc actgttt
//
LOCUS       DAOMIV010000150         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23572_length_3977_cov_4.020398, whole genome shotgun sequence.
ACCESSION   DAOMIV010000150 DAOMIV010000000
VERSION     DAOMIV010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23572_length_3977_cov_4.020398"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..1359)
                     /locus_tag="PKV59_04570"
     CDS             complement(49..1359)
                     /locus_tag="PKV59_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HQP46060.1"
                     /translation="MSKPTIISSPEILLPNKNVDLTKWATIACDQFTSNKAYWDEVES
                     IVGDSPSTLKITFPEYYLGKIDEAKKIQEIQDTMYRYLRDNLFVTHNGMILVERQIHN
                     GLRYGLLTAIDLEEYDYNRTSTSWIRATEGTVAERIPPRVRIREGAPLEVPHILVLID
                     DDQKRLIEPLIAQKESLPVLYDFDLMLNSGHVKGYSLADPELEQNIMDVIAKCQSDEE
                     ITRKYGPVSEKNRILFAIGDGNHSLATAKAIWEKHKSEWGMNHPARYALVEIENLHDP
                     VLEFEPIHRALFHETKSLISLLGDYFQDSMDVTEMKSMEQLFADIDRTPDGVQAFGVL
                     ESGKYYKVVLNRCSSALCVGSVQQFLDRCKSDHGFEEIDYIHGKEDLIHISSLEGNAG
                     IYLPPVQKNGFFQSIINDGALPRKTFSMGEAHEKRFYIECRRIL"
     gene            complement(1507..1581)
                     /locus_tag="PKV59_04575"
     tRNA            complement(1507..1581)
                     /locus_tag="PKV59_04575"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1544..1546),aa:Pro,seq:cgg)
     gene            2110..3468
                     /gene="ssnA"
                     /locus_tag="PKV59_04580"
     CDS             2110..3468
                     /gene="ssnA"
                     /locus_tag="PKV59_04580"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HQP46061.1"
                     /translation="MLIYNAKVVTWEKPNRILENSGVYIKDGVIQEVGSSDLLLKKYP
                     DEEKINADDQLLMPGSICAHTHFYGAYSRGLAIPGKPAKDFPEILDNLWYKLDRSLDE
                     ESTRLSAEVFIIDAIKSGCTTLFDHHASPEFIDGSLDVIADVVDKSGIRASLCYEVTD
                     RNGKDGTKAGIRENVRFLKRVKSGYTLDGRLSGTFGIHACLSVDEDTLSDCAAAIPEG
                     FGFHVHLAESQEDEWDSLYRFNQRCGKRLYDHHILGDKTIVAHAVHVDAAEMEMLKET
                     GTWISHQPRSNMNNAVGAAQVESMLANGMNVCLGNDGFTFDMWSEWKAAYFLHKVMNR
                     DPRRMNGYDVQEMAIYNNSRLAGQSFGYGEKFGKIVPGAPADIILVEYRPFDDLNAGN
                     IPWHIIFGFNERMITTTIVNGKILMKNRELTTMDEKEICARSLKKYPEVWKKFNAYHG
                     VK"
     gene            complement(3525..>3977)
                     /locus_tag="PKV59_04585"
     CDS             complement(3525..>3977)
                     /locus_tag="PKV59_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46062.1"
                     /translation="LSADYFLLFTEQYGLGITLFCGVHIARLYLFSTLKKRTEIIFWE
                     AFFASLIFFVCRSFIIAIASFYGFLILTVTIMSFRKLTFLADRYPVSKYLLRLGMILF
                     CLCDICVAVYNFPGSSYFISRLSGFLIWFFYLPAQLLLSFLIPPPLSK"
BASE COUNT         1233 a          757 c          863 g         1124 t
ORIGIN      
        1 aatttatatc gaaagtttca tacaattatt ttttgagcaa attccagcct ataaaatacg
       61 tctgcattca atataaaacc gcttttcatg tgcttcaccc atcgaaaaag tcttacgcgg
      121 taaagcaccg tcattaataa tactctgaaa aaacccgttt ttttggacgg gcggaagata
      181 gataccggca ttgccttcca aagaagaaat atgtattaaa tcctctttcc catggatata
      241 atcgatttct tcaaaaccgt gatccgactt acatcgatcc aagaattgtt ggactgagcc
      301 gacacataac gcagaagaac agcggtttag tactacttta taatattttc ccgattcgag
      361 aactccgaaa gcttgaacgc catccggcgt acgatcgata tcggcaaaca actgttccat
      421 ggatttcatc tcagtgacat ccatcgagtc ttggaaataa tctccgagaa ggcttattaa
      481 tgattttgtt tcatggaaaa gagcacggtg aatcggttca aattccagta ccggatcatg
      541 cagattttca atttcaacca aggcatagcg tgcgggatga ttcattcccc attccgattt
      601 atgtttttcc cagattgctt tagcggtcgc cagggagtga ttcccgtcac cgatcgcgaa
      661 gagaatcctg tttttttcac tcaccggacc atattttctg gtaatttctt catccgattg
      721 gcatttggca attacatcca taatattttg ttccagttcg gggtctgcta atgaatagcc
      781 tttcacatgt ccgctgttta gcatcaggtc aaaatcgtat agaaccggaa gagattcctt
      841 ttgggcaatc agtggttcta tcagtcgctt ttggtcatca tcgattaata cgagaatgtg
      901 aggcacctcc agaggagcac cttctcgaat tcgcacgcga ggcggaatac gctccgcaac
      961 cgttccttcc gttgctcgga tccaactggt cgaagtgcga ttgtaatcgt attcttccaa
     1021 atcgatggct gtaagtaacc cataacgtaa tccgttatga atttgtcttt caaccaatat
     1081 catcccgttg tgcgtaacaa ataagttatc tctcaaatat cggtacatcg tatcctgaat
     1141 ctcttgaatc tttttcgcct catcgatttt tccgagataa tattcgggaa atgtgatttt
     1201 caacgttgag ggagaatccc ccacgattga ttccacttca tcccaatagg ctttattgga
     1261 cgtgaattgg tcgcaagcga tggttgccca ttttgtcaga tcgacatttt tgttggggag
     1321 taaaatttcg ggactgctga tgatggttgg tttgctcatt tctttttccg ctccgatgaa
     1381 tgattattcg aatttcaagt aattgtaacg taaagatctg atccgtaaag gttttttgcc
     1441 tattgtaaga caattcatta aaatggaaat ttaaataaaa aacaccttgt ttagaggtgt
     1501 tttgtgtcgg ggcgggcgga tttgaaccgc cgacctcacg gacccgaacc gtgcactcta
     1561 tccggactga gccacgcccc gaacgacgtc cgttattata atctaccttt ttgattttat
     1621 gcaatgtcgg gagaaggggt gtgataattc taagtgtatc gagtgaaaga atttgatttg
     1681 gttcacagag aaagatattt cgtatacatc atagaaaaag ttgaatcgcg ccgctttggt
     1741 tcatcgagca ggtctttaaa aattttccat caacttcata acatcttttt atgccattat
     1801 tttttgacca aagaattata aatatctcgt tatacatgaa agatgtaatg agaaatagtg
     1861 ataaaaataa ccaataaaag attgtaagac aacttggaaa atgctacaat gaaaatacgg
     1921 aggtcggtgt attttattta catcggcgtt ggtgattccc atcgaaaagc gccgccggga
     1981 gactgtgagg agctgatgga ttttatccga tttactgaaa gactttttat acgaaataaa
     2041 gctcttctcg ttataaaaag tcttttaatt tcctgaagtc caaaattcaa gagaaaaaag
     2101 gagaaaataa tgttaatcta taacgctaaa gtagttactt gggaaaaacc caatcggatt
     2161 cttgaaaatt ccggtgttta tatcaaagat ggagtgattc aagaggtcgg ttcaagtgat
     2221 ttactgctga aaaaatatcc tgatgaggaa aaaataaatg ccgatgatca attattaatg
     2281 cccggtagta tttgcgctca tacccatttt tacggagcat attcaagagg attggcgatt
     2341 cccgggaaac cggcgaaaga ttttccggag atattggata atctttggta taaactggat
     2401 cgatctttgg atgaagaaag cacgcgttta tcagcggaag tatttatcat agacgcaatc
     2461 aaatccggtt gtacaacgct atttgatcac catgcatcac cggaattcat cgacggatcg
     2521 ttggatgtta tcgctgatgt ggttgataag agcggtatcc gggcttctct atgttatgaa
     2581 gtaacagacc gaaacggaaa agacggaacc aaggcgggaa ttagagaaaa tgttcgattt
     2641 ctgaagagag tcaaatccgg ttataccctt gacggacgtc tttcgggaac tttcggtata
     2701 catgcctgtt tatcggtaga tgaagacacg ctttcggatt gcgccgctgc gattccggaa
     2761 ggttttggat ttcatgttca tctcgccgaa tcccaggaag acgaatggga cagtttgtat
     2821 cggttcaatc aacgatgcgg gaaacggctt tatgatcatc acattttggg agacaagacg
     2881 attgttgcac atgcggtgca tgttgatgcc gctgaaatgg agatgttgaa ggaaacaggt
     2941 acatggatct ctcatcaacc gcgctcaaat atgaataatg cggtaggagc ggcgcaagtt
     3001 gaatccatgt tagccaatgg gatgaatgtc tgtctgggaa atgacggctt tacttttgat
     3061 atgtggagtg agtggaaagc cgcatatttt ctgcataaag taatgaatcg ggatccgcga
     3121 agaatgaacg gttatgatgt acaggaaatg gcgatttaca acaattcccg actcgcggga
     3181 caatccttcg gatacggaga aaaatttggc aagattgtcc ccggagcacc tgcggatata
     3241 atcctagtcg aatatcgacc atttgacgat ttgaatgcgg ggaatattcc ttggcacatc
     3301 attttcggat tcaatgaaag gatgattacg acaacaatcg tcaacggaaa gattttgatg
     3361 aaaaatcgcg aattgaccac aatggatgaa aaagaaattt gtgccagatc cttaaagaaa
     3421 tatcccgaag tttggaaaaa atttaatgct tatcacggcg tcaaataaaa tagaataaaa
     3481 atcaggaata aacaatccgc caaagatgga atttggcgga ttgtttattt tgataggggt
     3541 ggcggtatca ggaaagagag taacaactga gccggtaaat agaaaaacca tattaagaag
     3601 ccgcttaacc gcgaaataaa ataacttgaa ccgggaaaat tgtatacggc aacacagatg
     3661 tcacagaggc aaaacaaaat catgcccaac cgaagcaaat attttgaaac ggggtagcga
     3721 tccgctaaaa atgtcagctt ccgaaaagac attattgtca ctgtcaggat taaaaaccca
     3781 tagaacgaag ctatagcgat tatgaaggat cgacatacaa aaaagatgag agaagcaaag
     3841 aatgcttccc aaaaaataat ttcagttctc ttcttaagcg tcgaaaaaag atataagcgc
     3901 gctatatgaa ctccgcaaaa aagcgtaatt cccaaaccgt attgttcggt aaaaagaaga
     3961 aagtaatcgg cgctgag
//
LOCUS       DAOMIV010000151         3977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23574_length_3977_cov_3.848037, whole genome shotgun sequence.
ACCESSION   DAOMIV010000151 DAOMIV010000000
VERSION     DAOMIV010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23574_length_3977_cov_3.848037"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKV59_04590"
     CDS             complement(<1..724)
                     /locus_tag="PKV59_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46063.1"
                     /translation="MKELFPDLVRAAISTTANVLLMMTLLQPRYSKKITLLTMLGILA
                     ADLGTAIYCYLSGNLTLLSKLDIVLFAVLCFAVRPLFKDTFMQWLFSYLTVQNISDVV
                     IILSFIGSRHLPYPVYANSLLRFILFGVFLLILSRRVRPLYRQAVEHWTAYFAVALSI
                     YLTFNYYVLSSVDIVVTLTEQAIPLLLVIFISLAAYGSIFLSLKNLQQEFQIKEENRK
                     MQAERDYLQLAARNMSRRLELMA"
     gene            complement(724..1458)
                     /locus_tag="PKV59_04595"
     CDS             complement(724..1458)
                     /locus_tag="PKV59_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HQP46064.1"
                     /translation="MLRIAICDDMADELQILLSLTNQYISTNHLDVDVKGFTHPDQLL
                     TAIIIESFHLYILDVVMPMISGLELGKEIRRLDQEAQIIFVTTEPQFALQAYAANPIN
                     YLIKPIQRQQFFDTLTLVVSKADFAEEQTFAVKTAEGLRVIRLRDIVCCEYRCHAVIF
                     SLTNGEEILSRTFRENFSNYASTILKDRHFLQCHSSFVINLRRVERFTKENFTLTGGK
                     MIPIAAKQYPTVRDTYMDYLTGIGNR"
     gene            complement(1769..3340)
                     /locus_tag="PKV59_04600"
     CDS             complement(1769..3340)
                     /locus_tag="PKV59_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HQP46065.1"
                     /translation="MNKKILSVFLVLSVLLTVVFSAGAQDTAAETVKLTILGTNDIHS
                     YTADTPDKVAEGETAKTGSIGYAKIAAYKKQVAQNGPVLLFDSGDATHGQVIATLLEG
                     ESIIWLMNEAGYDAMTPGNHDFNYGYQKLIDFNYMADFPILSGNLVYADSQMSVLPEY
                     TIFDVDGIKVGVFGISTPETLYKSSPVNTEGLQFIDPYEAGKYYADFLRPHVDLVVCL
                     SHLGLDAGSEFTSEGLANAVPGIDVIIDAHSHTELPEGKLVGNTLIAQAGEHGRFIDQ
                     VEIELAAGQASTDRMTVVSKSAKLITYEDLLNLEPDPDVLEINTEIEKQINEISSVVI
                     GKTTVDLDGERETNRKQETNLGDLIAQAMLLETGADVAITNGGGIRASIPAGDVTLGQ
                     VITTFPFGNYVVTIEATGQDLIDAMENGLSDYPELKGAFPQIAGMRVVFDPSKPAGSR
                     VVELTVDGKPVDPAATYVVATNNFMAIGGDEYTMFADDKLLGEYSAFDEILADYLTTY
                     GTEGLEVDGRISTVQ"
     gene            3540..>3977
                     /locus_tag="PKV59_04605"
     CDS             3540..>3977
                     /locus_tag="PKV59_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQP46066.1"
                     /translation="MMQTTPLAARMRPRSLDEFVGQEHILGEGKLLRRAIESDKLFSS
                     VILFGPPGTGKTTLAQIIAAQSKAYFESVSAVLAGVADLRKVIVDAQERRKLYRTRTL
                     LFVDEVHRWNKAQQDALLPHVENGLITLIGATTENPYFEVIKAL"
BASE COUNT         1080 a          931 c          874 g         1092 t
ORIGIN      
        1 ccgccatcaa ttccaagcgt cgagacatat ttcgggccgc taattgcagg taatctcgtt
       61 ccgcctgcat tttccgattc tcctctttaa tttgaaactc ttgctgaagg ttttttagag
      121 agagaaaaat agatccatat gcggcaaggc tgataaaaat cactaaaaga agcggtattg
      181 cttgctccgt cagagttaca acaatatcaa ccgaagacaa aacatagtaa ttgaacgtga
      241 ggtaaatgct taatgccaca gcaaagtatg ctgtccaatg ctcaaccgcc tgccggtaca
      301 acgggcgtac ccgtcgggat aaaatcaaca gaaaaacacc gaacaggatg aaacgcagca
      361 gcgagttggc ataaaccgga taaggaagat gtcttgagcc gataaaactg aggataatca
      421 caacatcgct gatgttctgt acggttagat aggaaaacag ccactgcatg aaggtatctt
      481 taaataatgg tcgaacagca aagcatagaa ccgcaaacaa aacaatatct agctttgaaa
      541 gcaaggttaa atttccgctg agataacaat aaattgccgt gcccaaatca gcagccaaaa
      601 tacccagcat agtcaacagt gtaatctttt tggaatatct cggctgtaaa agcgtcatca
      661 tcagaaggac gttggcggta gttgagattg ccgcccgcac caagtcggga aaaagttctt
      721 tcattaacgg tttcctattc ctgtcagata gtccatatat gtatctcgaa ctgtaggata
      781 ttgcttcgcc gcgatgggta tcattttccc gccggtcaat gtgaagttct cctttgtgaa
      841 acgctccacc cggcgcagat tgattacaaa tgaggagtga cattgtaaaa aatgcctgtc
      901 cttcagaatt gttgaggcgt aatttgagaa attctctcga aatgtccggc tgagtatttc
      961 ttcaccgttt gtcaaactga agataacagc gtgacagcga tattcgcagc agacgatgtc
     1021 ccgcagtctg ataacccgca gtccttcagc agttttgacg gcaaatgttt gctcttccgc
     1081 aaagtctgct ttagagacga ccagtgttaa agtatcaaaa aactgttgtc tttggattgg
     1141 tttaataaga tagtttatag gattcgctgc ataggcttgt aaggcaaatt gcggctccgt
     1201 agtaacaaag ataatttgtg cctcctgatc caagcggcgt atttctttac ccaattccaa
     1261 cccgcttatc atcggcatga caacgtctaa gatataaaga tgaaagcttt ctatgatgat
     1321 agcggtgagt aattgatcgg gatgagtaaa cccctttaca tcgacatcaa gatggttagt
     1381 tgagatgtac tgatttgtca acgagagaag gatttgcagt tcgtctgcca tatcgtcgca
     1441 gattgcaata cggagcatga cagtggcctc ttttcaatgg ggatttgaaa atattttatc
     1501 attgtttttt tctccgtctc cggaaacccc ggtgtctcaa cttttttggt tttctcccta
     1561 ctacttcgac tccataccga tcgaaccgtt ccgtaaacca gatatcatat tcacgaaaag
     1621 aagacaggac gtttctaaac cgcaccttat cccatatgtc taaaaaaaag ggcgaacagt
     1681 cacaactgcc cgcccccgga aaaagatcct gccccgattt tctttttgtc cctataacaa
     1741 catcatttaa aatcgggctc tgttccgttt attgaaccgt agagattctt ccgtcaactt
     1801 caagcccttc ggtgccgtat gtcgtgagat aatccgccag gatttcatca aaagcggaat
     1861 attcaccgag caatttatca tccgcaaaca tggtatattc atccccgccg atcgccataa
     1921 aattgttggt agcaaccaca taagtcgccg ccggatcgac cggttttcca tccaccgtga
     1981 gttcgacgac acgactgccg gccggtttgg aaggatcgaa tacgactcgc attcccgcga
     2041 tctgagggaa tgcgcctttc agctccggat aatcactcag accgttttcc atggcatcga
     2101 tcaaatcctg tccggtggct tcaatcgtta caacatagtt gccgaacggg aatgtggtga
     2161 ttacctgacc taacgtcaca tcgccggcgg gaatcgatgc tcgtattcct ccgccgttgg
     2221 taatcgcaac gtccgctccg gtttcaagca acattgcctg agcgatcaga tctcccaagt
     2281 tggtttcttg tttccggttt gtttcccgtt cgccgtccaa atccaccgtg gtttttccga
     2341 tcaccacgga tgaaatctca ttaatctgtt tttcgatctc ggtattgatt tcaaggacat
     2401 ccggatccgg ttcaagattt aagagatctt cataggtgat gagttttgcg gattttgaca
     2461 caacggtcat ccggtcggtc gaagcttgcc cggctgccag ttcgatttca acctgatcga
     2521 taaagcgtcc gtgttcgcct gcttgagcga ttaaggtatt ccccaccaat ttcccctccg
     2581 gtaattccgt gtgactgtgg gcatcgatta tgacatcgat tccgggaacc gcatttgcca
     2641 agccttcact ggtaaactca ctgccggcat caagccccaa atgagacagg caaacgacaa
     2701 gatcgacatg aggacgcaga aaatccgcat aatattttcc cgcttcatag ggatcgataa
     2761 attgcagacc ttcggtgttc accggtgagg atttatataa tgtttcgggg gtggaaattc
     2821 caaagacacc cactttgatt ccgtcgacat caaaaatcgt gtattcggga agaacactca
     2881 tttgtgaatc ggcataaacc agattcccgg aaaggatcgg gaaatccgcc atgtagttga
     2941 aatcaatcaa tttttgatac ccgtagttga aatcatgatt tccgggagtc atcgcgtcat
     3001 aaccggcttc attcatcaac caaatgatcg attctccttc caagagagtg gcaataacct
     3061 gcccatgggt ggcatcaccg ctgtcaaaaa gcaataccgg accgttttgg gcaacctgtt
     3121 ttttataagc ggcaatttta gcatatccga tggatccggt cttagctgtt tccccctcgg
     3181 cgactttgtc cggtgtgtct gccgtatagc tgtggatatc atttgtgcct aaaatggtta
     3241 attttacggt ctccgctgcg gtatcctgcg caccggccga aaaaacaacg gtgagcagaa
     3301 ctgatagaac caagaaaact gataagattt ttttattcat ttctccttct ttctcctttt
     3361 tatcgattta aaataggttg ctcagccctt tgtagaatca aataaagaat atttgattat
     3421 taatagtata ccttcaatac cttgaatatg ttgatccgct tttcatctgc ggaattttta
     3481 gccgtagagt ataatttttt tgagatatgg acctttttga ctatacggat cgggaaaaca
     3541 tgatgcaaac aacccccctg gctgcacgga tgcgcccgcg gagtttagat gagtttgtcg
     3601 gacaggaaca tattctggga gaaggaaaat tacttcgaag ggcaatcgaa tccgacaaac
     3661 tcttttcctc cgtcatcttg ttcggtcccc cggggacggg gaaaaccaca ttggcgcaaa
     3721 tcatcgccgc tcagtcgaaa gcttattttg aatcggtttc ggctgtcttg gcaggagtcg
     3781 ccgaccttcg taaagtgatt gttgacgcac aggaacggag gaaattgtat cggacccgga
     3841 cactcctttt tgtggacgaa gttcatcgtt ggaataaggc acagcaggat gctctgctgc
     3901 cgcatgtcga aaacgggttg atcactttga tcggcgcgac caccgaaaac ccctattttg
     3961 aggttattaa agcatta
//
LOCUS       DAOMIV010000152         3973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23597_length_3973_cov_3.382083, whole genome shotgun sequence.
ACCESSION   DAOMIV010000152 DAOMIV010000000
VERSION     DAOMIV010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23597_length_3973_cov_3.382083"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..825)
                     /locus_tag="PKV59_04610"
     CDS             complement(<1..825)
                     /locus_tag="PKV59_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQP46067.1"
                     /translation="MRKEFWRSFDFWLFGDIVILCVFGIVMIQSALAGNLEVSVNSQI
                     IYVCLGFVLIMILTLIDYHYLESLANLLYFGIILVLLTLNLFGQATFGSARWLQIGPF
                     SLQPSEFAKTILIIRLAKYFSDHANSKHDMRWILKSFALTFGIVFFILLQPNMSTSIV
                     MMVIWFSMLWVSKIPTKYLIILGTVGVILLIGITIWIFVSPDTIPFIQEYQLNRLRNF
                     FNPDENARYGDKYNVDQAVISLGSGKLFGQGYGNGTQVRLRFLKVRHTDFIFSVLGE"
     gene            complement(873..2966)
                     /gene="mrdA"
                     /locus_tag="PKV59_04615"
     CDS             complement(873..2966)
                     /gene="mrdA"
                     /locus_tag="PKV59_04615"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQP46068.1"
                     /translation="MNEFNREKLDFKNRRFVAFYVVMGAIILFFIYRLVVIQLVEGDY
                     YLSLADENRISIVKEQTTRGIIYDRNGVVLARNVPTYDIVITPANLPTDEGDIQAIYR
                     KLSEILDIPVNNGVINEETVRLYSECGTDFGISQIIYIANSLTPYAETAIKCDVPEET
                     AMIVQELAANMPGVGVRVNSIREYPTGYDTSEVIGFLGPIPDTVADEMESRGFILNKD
                     KYGYAGVESSLQDLLSGKNGRRVVEKDVAGLETRDLEPPKDPVPGYNVKLTIDVRLQN
                     VARAAMLDTMDFYNRISVTGPLTHDGAAIAMNPQTGEILALVSEPTFENNRMTRYIPA
                     YYYQQLSLDQSKPLLNHATQVAQPPGSVFKIVTGIGAINEKVVSPDFHINCPGHIFIQ
                     NSYSPNDPGSEQEYVCHLRTGHGQVDYLHALAWSCNLYFNKVGGGYKDEVPEGLGIDR
                     LGQYAHALGYGEISGIELTGEETGMIPTRTWKRVNKGESWATGDTYLASMGQGYISAS
                     PLQVLGSFVTVANKGIHIKPTIIKEVLDDRGNVVLPFTPKMLWDITKDPKIMVYDENG
                     EETGKYKTVDPTVIDLTRQGLRLVTQPGGTASEAFAGDTHQSSAKTGTAEYCDDIAMA
                     KDACKFGNWPSHAWTVAYAPYDNPEIAILVFVYNGKEGAYMAGYPVRRILDNYFLLKE
                     YDAERTGETSGSLIQ"
     gene            complement(2966..3469)
                     /locus_tag="PKV59_04620"
     CDS             complement(2966..3469)
                     /locus_tag="PKV59_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46069.1"
                     /translation="MKEIISSFVILILGGILQGSLFSRIHILNGTGDVIMVMLIVWSL
                     NPYTRYSWIWLLLGALIMTYLSALPLYGYFFIYGIIWLFIRFLKSRFWQMPIILMIFV
                     TITGSIISAVISYFVLYINDISPNLKTTVTQIAVPSLVLNLIYCFPIYGLLNDFANSV
                     CYKEESS"
     gene            complement(3487..>3973)
                     /gene="mreC"
                     /locus_tag="PKV59_04625"
     CDS             complement(3487..>3973)
                     /gene="mreC"
                     /locus_tag="PKV59_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQP46070.1"
                     /translation="AAAVIGVDPSPFLQYILIDKGSDDGIAYNMPVVTEKGLVGRISA
                     VTASAARVQLITDAGSLVNAHVVEADADGVVRGSVTADLTIEMVSPEAELQAGQIVQT
                     SGLGGNFPAEVVIGQILNVNKLENELFQSASIQPAEDFSNLQAVLVVANFRPSNVEPL
                     E"
BASE COUNT         1226 a          941 c          716 g         1090 t
ORIGIN      
        1 ctcacccaat accgaaaaaa taaagtccgt atgacgaacc ttcagaaacc gtaaccgaac
       61 ctgtgtacca ttgccgtaac cttggccgaa taattttccg gatcctaacg aaataaccgc
      121 ttggtctaca ttatatttat caccgtatct ggcgttttca tccggattaa agaaatttct
      181 cagccggttt aattgatatt cctgaataaa cggaatcgta tccggcgaaa caaaaatcca
      241 gatggttatc ccgatcaaca agatcactcc gacggttccg aggatgataa ggtatttcgt
      301 cggaatttta ctcacccaca acattgagaa ccaaattacc atcatcacga tagaggtact
      361 catatttggt tgcagcaaaa tgaaaaaaac tattccgaaa gttaaagcaa agctttttag
      421 aatccatcgc atatcgtgct tcgaattagc atgatccgaa aaatatttag ccaagcgaat
      481 aatgagaatt gtttttgcaa attcagaggg ttgtaatgag aagggtccta tctgtaacca
      541 tctggccgat ccaaaagtag cctgcccgaa taaattcaaa gttaacaata ccaagataat
      601 cccaaaatac aacaaattag ccagtgactc aagatagtga taatcgatca aagtcaaaat
      661 catgatcagg acaaatccca aacatacata aatgatctga ctgtttacac tgacctcaag
      721 gttacccgct aaggcagatt gaatcatcac aataccgaaa acgcaaagga tgacaatatc
      781 cccgaaaagc caaaagtcaa aactacgcca aaactctttt ctcattcgtt cttaatcttt
      841 ttcaaacctt atctttttta tacgcataaa tcttattgaa tcaaagaacc cgacgtttcg
      901 ccggtccgct cggcatcata ttctttcaaa agaaaatagt tatccaaaat tcttcgtacc
      961 ggatatcctg ccatataagc gccctctttt ccgttataaa caaatacaag tatcgctatt
     1021 tccgggttgt catagggtgc ataagctacc gtccaagcgt gcgaaggcca gtttccgaat
     1081 ttgcatgcat ccttcgccat cgcaatatca tcgcagtatt cagcggttcc ggtcttagct
     1141 gaagattgat gtgtatcacc ggcaaaagct tcgctggcgg ttcctcccgg ttgggtcact
     1201 aatcgcaatc cttgccgggt tagatcaatt accgtcggat caaccgtttt atatttcccg
     1261 gtctcctctc cgttttcatc ataaaccata attttggggt cttttgtgat atcccataac
     1321 attttcggag taaaaggaag aacaacattt ccgcgatcat ccaatacttc tttaataatt
     1381 gtgggcttaa tatgaattcc tttattagcg acggtaacaa acgaaccgag aacctgcaaa
     1441 ggagaagcgc tgatataacc ttgccccata gaagccaaat aagtatcacc tgtcgcccaa
     1501 gattcccctt tattcaccct tttccaggtt ctggtcggaa tcataccggt ctcttctccc
     1561 gttaattcaa ttccggaaat ttctccatat cccaatgcgt gagcatattg tccgagccgg
     1621 tcgattccta atccttcagg aacttcatct ttgtatccgc cgccgacttt attaaaatac
     1681 aagttacatg accaagcaag cgcatgcaaa taatcaacct gcccatgacc ggtccgtaaa
     1741 tggcatacat attcttgttc cgaaccggga tcattcggag aatagctgtt ttgaataaaa
     1801 atatgtccgg gacaattaat atggaaatca ggcgaaacca ctttctcatt gatagcgcca
     1861 ataccggtaa caattttaaa tacggagccc ggaggttgag ccacctgagt cgcgtgattc
     1921 agtaacggtt tggactgatc caacgatagt tgctgataat aataagccgg gatataacgg
     1981 gtcattcgat tattttcgaa agtcggttct gagactaaag ccagtatttc tcccgtttgc
     2041 ggattcatgg caatcgcagc tccgtcatgt gttaaaggac cggtgacact gatccgatta
     2101 taaaaatcca tcgtatccaa cattgcggct ctcgcgacat tttgcaaacg gacatcgatt
     2161 gtcagtttca cattgtagcc cggaaccgga tcctttggcg gttccaaatc ccgagtttcc
     2221 aagccggcaa catctttttc aacgactcga cgcccgttct tcccggataa caaatcctgt
     2281 aaggaagact caacacccgc ataaccatat ttatccttat ttaatataaa gccgcgactt
     2341 tccatctcgt cggcaaccgt atcgggtatg ggaccgagaa atcctatcac ctcggaagta
     2401 tcataccccg ttggatattc tcgaatcgaa ttgacccgaa ccccgacgcc gggcatattt
     2461 gccgctaatt cctgaacaat cattgcggtt tcttccggca catcgcattt aatcgcggtc
     2521 tccgcgtaag gagtcaacga gttagcaata taaataattt gagatattcc gaaatccgtc
     2581 ccgcattcac tgtaaagccg cacagtctct tcattgatta caccgttatt tacaggaata
     2641 tcgagtattt cggatagctt tcgataaatg gcttggatat ctccttcatc agtcggaaga
     2701 tttgccggtg taataacaat gtcatacgta ggtacattcc tggccaggac tactccgtta
     2761 cggtcataaa taattccgcg ggtagtttgt tcctttacaa tcgatattcg attttcatcg
     2821 gctaaactaa gataatagtc tccttctacc agttgaatga caaccaatct atatataaaa
     2881 aagaggataa tagcacccat caccacataa aatgctacaa atcttcggtt tttaaaatcc
     2941 agtttttctc gattaaattc attcattatg aagattcttc cttataacaa acagaattcg
     3001 caaaatcatt cagcagtcca taaattggga aacaatagat tagatttaat accagtgacg
     3061 gcaccgcaat ttgagttacg gtggttttca aattcggtga aatatcgttg atatacaata
     3121 caaaataaga aatgacagct gaaataatcg aacctgttat tgtcacaaat atcattaata
     3181 tgataggcat ttgccaaaag cgacttttca gaaaccttat aaacagccaa ataattccat
     3241 aaatgaaaaa atatccatat aaaggtaaag ctgacaaata agtcattatc aaagctccta
     3301 aaagaagcca aatccatgaa taacgggtat acggattgag tgaccaaaca atcaacatta
     3361 ccatgatcac atcaccggta ccgttcaaaa tatgtattcg actgaacaag gatccttgta
     3421 ggattccacc taagatcagg ataacaaatg aggaaataat ttctttcatt gaatcgacaa
     3481 ctatctctat tccaaaggtt ccacattcga agggcggaaa ttcgccacca ccaaaactgc
     3541 ctgaagatta ctaaaatcct ctgccggttg aatggatgcc gattgaaata gttcattttc
     3601 cagcttattc acatttaata tctgcccgat caccacttca gcaggaaaat ttccccccaa
     3661 tcccgaagtt tgtacaattt gtccggcttg caattccgcc tcgggagata ccatttcaat
     3721 tgtaaggtcg gcagtcaccg aacctctgac gactccatcc gcatccgcct cgacaacatg
     3781 ggcgttcact aacgaaccgg catcggtgat taattgaact ctggcggcgg acgcggtgac
     3841 ggcgctaatt cttccaacca atcctttctc tgtaacaacc ggcatgttat aagcaatccc
     3901 gtcatcggag cctttatcaa ttaggatata ttgcaaaaaa ggacttggat cgacaccgat
     3961 tacggcagcc gca
//
LOCUS       DAOMIV010000153         3964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23659_length_3964_cov_4.253773, whole genome shotgun sequence.
ACCESSION   DAOMIV010000153 DAOMIV010000000
VERSION     DAOMIV010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3964
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23659_length_3964_cov_4.253773"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..1026)
                     /locus_tag="PKV59_04630"
     CDS             complement(274..1026)
                     /locus_tag="PKV59_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HQP46071.1"
                     /translation="MNWEILISLIMSYLIGSIPFGWIIVWIKKHQDIRYHASGKMGTS
                     NVIRMTDVPTGIITFFLDLFKGYAGVWVVRLIMDPAPAWMEAVGGFLAVLGHVYSIFI
                     VEKRRDGRYYFRGGAGGMTSMGAAIALWPPVILYSGIPSLLIYFIFGYASVATMSINV
                     VALICFIVRAAFGLDPTWWYAVYAFFTLLLVLKALRPNIRRLRRGEERVMGFSIRGKI
                     KAKKEAERAKSSSDKFAVSPDKKKTDQVKTRS"
     gene            complement(1043..1858)
                     /locus_tag="PKV59_04635"
     CDS             complement(1043..1858)
                     /locus_tag="PKV59_04635"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46072.1"
                     /translation="MNEENQIGNDYSILTVIILLVYVLSTFITSYIVTIPIHIGNFSL
                     ILNWKMIDLITPGAGLLAGIGVFGLVDERSSKSKAEKMLHSVIPFATTVSLGIILRNV
                     AMSAGWVMILILGGVLLYLVFMSECIICNPADPRISVVSILLTGLAYATFVITAIGVR
                     SNISRLILEFPLILLSSFILAMRILSLYESVKDIEIKAAIISLITAEAAAAFHYWPIQ
                     PLSYSLLLFLGFYILTNIVIILPQKGSLRNAFKSQLWILAVSIILMVFIEIQK"
     gene            2084..2614
                     /locus_tag="PKV59_04640"
     CDS             2084..2614
                     /locus_tag="PKV59_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46073.1"
                     /translation="MPKKKILLFILIGVIVFSFFVIACQTRISDSGYSLKTVGKKIDS
                     NIYILDVTMLGPVTSIKDLSAEGTAKQYSDIYGYGNMRLWQDGKGLVSVHVNSINPKL
                     DYVQPGTDIVLKTTDLKILAIAEGYRFEVKCRNDYEPVASLEDNEITSERYDTYELDY
                     CRMTTPEITLEKNIEE"
     gene            2616..2969
                     /locus_tag="PKV59_04645"
     CDS             2616..2969
                     /locus_tag="PKV59_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQP46074.1"
                     /translation="MDRIILKDLLARGRIGISDKERERPQDILINIEMQTDIHPAGLS
                     DNLLDCVDYSETAKAVLALVEDNNRKTLEALTTDIADYCLQHPLVRSVKVRVEKTAAV
                     RFAKSAGVEIEREKK"
     gene            2994..3683
                     /locus_tag="PKV59_04650"
     CDS             2994..3683
                     /locus_tag="PKV59_04650"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HQP46075.1"
                     /translation="MLKIIAFILFAFLICGCDSIVLSTPSPTPDFEKTVYWLVDEAVS
                     KASTQIMFDVDQKLATMEAKQPSLIENITETPTPTASVVAAPFRGTSVPEALRKKENS
                     VVENTPASCIDSLKFVEDITIPDKTVVKAGKPFTKTWKIQNTGTCTWTEAYSVVFSDG
                     DKMSALDRTGLPVGTSVKPNDTIEVSVYMIAPEQSGSYAGYWLFESPSGIRFGAGSNR
                     DKPIWVKVEVK"
     gene            3680..>3964
                     /locus_tag="PKV59_04655"
     CDS             3680..>3964
                     /locus_tag="PKV59_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46076.1"
                     /translation="MKKFALLSGLIFLSLVSGCTYHSPEPINLDATIQAGISSALEEK
                     DLLDQAKLDQSQQTQNTQIAAEVDRKVSSLATEVYLDMQENVLPEIGGTPT"
BASE COUNT         1262 a          855 c          789 g         1058 t
ORIGIN      
        1 gatgaggatg acttttatga ctccgatgaa gactttgatg atgattttta ataaagaatg
       61 atcaacttat catttgccct tatggagact aaataagtta aagcaaagat tgattacttt
      121 gttttaactt attttcatca gctatattac ccaattagta catttcctca aatgatattg
      181 cctgctatct cctctttata caagtatcga atttactcat attaatcaga tatgaaataa
      241 gatttccagt cattaattta tttttattct taattaagat ctggtcttaa cttgatccgt
      301 tttcttctta tccggtgata ccgcaaattt atcggaggaa gattttgctc tttcggcttc
      361 ttttttcgct tttatttttc cccggatact gaaacccatc acgcgctctt ccccgcgtcg
      421 taatcgtctg atattcggcc gaagagcttt cagaaccagc aaaagcgtga aaaatgcata
      481 aaccgcatac caccaagtcg gatccagccc gaatgccgcc cgaacaataa aacaaatcaa
      541 cgccacgaca ttgatcgaca tggtggcgac agaggcatat ccaaaaataa aataaatcaa
      601 aaggctcgga attccgctgt aaaggatgac gggaggccac aaagcaatcg ccgcccccat
      661 cgatgtcatt ccgccggcac ctccgcgaaa atagtacctg ccgtcacgcc ttttttcgac
      721 aataaatatc gaataaacat gaccgagcac ggctaaaaag ccgccgaccg cttccatcca
      781 tgcgggagcc ggatccataa tcaaccttac cacccaaaca ccggcatacc ccttaaaaag
      841 atcaaggaaa aaggttatga ttccggtagg cacgtccgtc atccggatga cattcgaagt
      901 gcccattttc cctgatgcat gataccgaat atcctgatgc ttcttaatcc aaacaatgat
      961 ccaaccaaat gggattgaac cgatcaaata gctcatgatt aaagatatga ggatttccca
     1021 attcatttca attccttaaa gactattttt gaatttcaat aaataccatt aatataatcg
     1081 ataccgcaag gatccaaagc tgacttttaa aagcatttcg caaggaaccc ttctgtggaa
     1141 gtattataac gatattggtt aaaatataaa aacctaaaaa caataaaagg ctgtaagaaa
     1201 ggggttgaat cggccaataa tggaaggcgg ccgccgcttc cgcggtaatt aacgaaatga
     1261 tcgcggcttt tatttcgata tctttaacgg actcatacaa agataagatc ctcatcgcca
     1321 gaataaatga agacagcaga atcaacggga attccaaaat cagcctggaa atattggatc
     1381 gtacaccgat ggcagtaatt acaaatgtcg cataagccag accggttaac aggatcgaaa
     1441 ccaccgaaat tcgcgggtca gcgggattgc aaatgataca ctcggacata aacactaaat
     1501 aaagcaggac accgccaagg atcaaaatca taacccaacc cgcagacata gcaacatttc
     1561 tcagaatgat tcccaatgaa accgttgtcg caaacgggat aacggaatgg agcatttttt
     1621 ccgctttgct tttcgacgac cgttcatcga ctaaaccaaa tacaccgatc cctgccagca
     1681 accctgcgcc gggtgtgatt aagtcaatca ttttccaatt tagaatcaga gaaaaattac
     1741 cgatgtgaat ggggatcgtt acaatatagc tggttataaa agtgctcaat acataaacca
     1801 gcaggataat cactgtcaaa atgctatagt cgttcccgat ttgattttct tcattcatat
     1861 tcaagcttaa ttatatcgaa tcaaattttc ttatttgcat acaatcaact ccgataaatc
     1921 gaaaacatcc tctccttccg gataaaagtc cgcgtcttat ttttcggttg aaaaagtttg
     1981 caatcttttc aaaaaatcag ctaaacttat tttgagattt gtatcacttt ttcgcttaaa
     2041 tcgagcggaa actaatagaa tagaatcaaa cggggagcaa aaaatgccaa agaaaaaaat
     2101 attactcttt attctgatcg gagttatcgt tttctctttt ttcgttatcg cttgtcaaac
     2161 ccgcatcagc gacagcggat acagtttgaa aacagtcggg aaaaaaattg acagtaatat
     2221 ttatatactg gatgtaacaa tgcttggacc ggttacgagt attaaggatt taagtgccga
     2281 aggaacagcc aaacaataca gtgatatcta cggatatggg aatatgcgct tatggcaaga
     2341 tggaaagggg ttagtctccg ttcatgtcaa cagcattaat ccgaaactcg attatgttca
     2401 acccggtacc gacattgttt taaagaccac cgatttgaag attttggcta tcgcggaagg
     2461 gtatcgcttt gaagtaaaat gcagaaatga ttatgaaccg gtcgcgtctt tggaagataa
     2521 tgaaataaca tccgaacgtt atgatacgta cgaattggat tattgtcgga tgacaactcc
     2581 agaaataacc ttggaaaaaa atatcgagga ataaattgga tcgaattatt ctcaaagatt
     2641 tactggcacg cggtcggatc gggatttcgg acaaggaaag agaacgtccg caggatattc
     2701 tgatcaacat cgagatgcaa accgatatac acccagccgg gctatcggac aatttattgg
     2761 attgtgtgga ttacagtgaa accgccaaag ctgtattggc tttggtcgag gacaataatc
     2821 gaaagacatt agaagctctg acgaccgaca ttgctgatta ttgtcttcaa catccgttgg
     2881 tccgatcggt aaaagtgcgc gtggaaaaaa ccgccgctgt ccgatttgcc aaatcggcag
     2941 gagtggaaat cgaaagagaa aaaaaataat tcaaaatgca agtcaatccg ctaatgctta
     3001 aaatcattgc ctttattctt ttcgctttct tgatttgcgg atgtgattcg attgtgcttt
     3061 cgaccccttc tcccacaccg gatttcgaga aaacagtcta ttggttagtg gatgaagcgg
     3121 tctctaaagc ttccactcag ataatgtttg atgttgacca aaagctcgcg acaatggaag
     3181 caaaacaacc gagcctgatt gaaaatataa ccgaaacccc gactccgacg gcatccgttg
     3241 tcgcagcacc gtttcgggga acttcggtac cggaggcttt aagaaaaaaa gaaaacagcg
     3301 tagttgaaaa tacacccgct tcctgcatcg attctttgaa atttgtcgaa gatatcacaa
     3361 tccccgacaa aaccgtagtg aaagccggta aaccctttac gaagacctgg aaaattcaaa
     3421 ataccggcac ttgtacctgg accgaggcat attccgttgt attcagcgac ggtgataaaa
     3481 tgtccgcatt ggatcggacc ggtttaccgg tcggtacttc tgttaagcct aatgatacaa
     3541 ttgaagtatc tgtttatatg attgcccctg aacaaagcgg atcctatgcc ggatattggt
     3601 tattcgaaag cccttccggc atccgttttg gagccggttc aaaccgggat aagccgattt
     3661 gggttaaagt ggaggttaaa tgaaaaaatt tgcgctttta agtggattaa tattcctttc
     3721 tttggtatcg ggatgtactt atcattcacc ggaacctatc aatttggatg ccaccattca
     3781 ggccggtatc tcctccgctt tggaagaaaa ggatctcttg gatcaagcga aattggatca
     3841 atcccaacag acacaaaata cccaaatcgc agccgaggtg gaccggaaag taagcagcct
     3901 ggcaaccgaa gtttatctcg atatgcagga aaacgtcctt cccgaaattg gcggtactcc
     3961 gact
//
LOCUS       DAOMIV010000154         3935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_23919_length_3935_cov_5.165722, whole genome shotgun sequence.
ACCESSION   DAOMIV010000154 DAOMIV010000000
VERSION     DAOMIV010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23919_length_3935_cov_5.165722"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PKV59_04660"
     CDS             complement(<1..236)
                     /locus_tag="PKV59_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQP46077.1"
                     /translation="MNETIQTLMNRKSVRKFTDQSVSDDTKQIILEAALHAPSAGAMM
                     LYSIIEVEDQALKDILAESCDHQPFIATAPWLLL"
     gene            342..821
                     /gene="rnhA"
                     /locus_tag="PKV59_04665"
     CDS             342..821
                     /gene="rnhA"
                     /locus_tag="PKV59_04665"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF001236.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQP46078.1"
                     /translation="MNDKRTKVTIYADGACLGNPGPGGYGVILICGDQKKELSGGAAL
                     TTNNRMELTAVIEGLKALKRPCEVLLYSDSKYVVDMVEGKWPQRWQKNNWMRNKKEPA
                     KNVDLWEELLRLLEIHSVTMKWVKGHAENALNNRCDELAVKAAESFKNSNHNNLSSR"
     gene            872..2083
                     /gene="xseA"
                     /locus_tag="PKV59_04670"
     CDS             872..2083
                     /gene="xseA"
                     /locus_tag="PKV59_04670"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQP46079.1"
                     /translation="MQNFDFFSFADFALSVSEVNQQVKDLLESDPVLEDISVKGEISN
                     LSRPVSGHVYFTLKDEKSSLKCVMWRTVAAEFQELFKNGAAVIVHGRIGVYERDGLYQ
                     LYADAVYPVGRGKLFEEFLRLKEKLEKEGLFDSGRKKPLPYMPERIGVVTSSSGAAIQ
                     DIFNTIRKRWPLAEIYLSPASVQGEDAPAELIKAYKKLVEMNPDVILIARGGGSLEDL
                     WAFNDETLVRTIASSKIPVISGVGHETDFTLLDFAADFRASTPTAAAVAATPDRLEII
                     QAVDFLTVKMNRLMREKLDKTAEKINQLNRRFELAAPLNQIRQEQKILEQIHIRLNLW
                     ADRYFKQQRELLIGLSRRLESAGPSSVLNRGYALIEDENGKIIVSASCFYSDEKVTIR
                     MKDGDRDAMIL"
     gene            2099..2329
                     /gene="xseB"
                     /locus_tag="PKV59_04675"
     CDS             2099..2329
                     /gene="xseB"
                     /locus_tag="PKV59_04675"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQP46080.1"
                     /translation="MENSSEKLTFKEAFEQLQKIVEQLESETVELEQAMELYKAGMQL
                     SKYCSSLLEQAELKIRTIGQTTVNGDKDSVSD"
     gene            2316..2768
                     /locus_tag="PKV59_04680"
     CDS             2316..2768
                     /locus_tag="PKV59_04680"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HQP46081.1"
                     /translation="MFLTEFFGNPAVVSGLAAMMLAQLLKLLLNYALNKKWDWVLLIE
                     SGGMPSSHSAMVTAIAVSIGLWEGFDTAAFAAAFGLMVIVTYDSANVRWQSGLHAQKI
                     NQLLRDVFSGQPINDQLLKEVIGHTPRQVYIGVILGILVALAIDSLWV"
     gene            2783..3805
                     /locus_tag="PKV59_04685"
     CDS             2783..3805
                     /locus_tag="PKV59_04685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQP46082.1"
                     /translation="MNKRIHVLGGAAIILGAILVGLILSVFLSNNHRVRASQTTGLPT
                     VTLISEMIPTHTTVLPTETMTPSPVVPSAIPTVQPTALSAADWKNWPILPERVSTKMI
                     DVYRAGQENGNKANRFSKVGDSNSIMPSFLGCFDYGENGYKLGKYAALEETIKQFQWS
                     FSRESRATANGITAMQLDTYHWYEDDVCWPYESATSCEYRLWQPSIAFIALGTNDVYM
                     PLAEFDKHMRSLVQKSIDRYVVPILVTKADNLEGDGSFNQAIAQIALDYEVPLWNLWR
                     AMDPLPGHGLRENDVHPTFNNTSLCDFSGDDLQTYGWTVRNLTGLQALDRVWHLLNQG
                     MTSSSQ"
BASE COUNT         1115 a          726 c         1010 g         1084 t
ORIGIN      
        1 aataaaagca gccacggggc agttgcgata aacggttggt gatcgcaact ctccgcaagg
       61 atatctttca aggcttggtc ctcaacttcg atgatagaat aaagcatcat tgcgccggca
      121 gagggcgcat gcaaggctgc ctctaggatg atttgcttgg tatcatctga aacagattga
      181 tcggtaaatt ttcggacgga tttccgattc atgagagttt gaattgtttc gttcataggc
      241 ttattttaat cgttattcga ttttattggg ttgatgatca aaaaatgcgg aatcataaag
      301 gatgctttta gagaagagta agaggagatg atccgatatc gatgaatgac aaacgaacca
      361 aagtaaccat ttatgcggat ggtgcttgtc tggggaatcc cggtccgggt ggatacgggg
      421 tgatattaat ttgcggcgat caaaagaaag aactttcggg tggcgccgct ctgaccacga
      481 ataatcgaat ggaattaaca gccgtgattg aaggtctgaa ggcattaaaa cgtccgtgcg
      541 aagtgctttt atattcggat tcaaagtatg tggtggatat ggttgaagga aaatggccac
      601 agcgctggca aaaaaataat tggatgcgaa ataaaaaaga gccggctaaa aatgttgatt
      661 tgtgggagga actcttacgt ttactggaaa tccattccgt cacaatgaaa tgggtaaaag
      721 ggcacgcgga aaatgcgctg aataaccgtt gcgatgaact ggcagtgaaa gcagccgaaa
      781 gttttaaaaa ttcgaatcat aacaatttgt catcccgata gatattatca aaagaatttc
      841 aagtgaaaac taccggtgat atactggttt tatgcaaaat tttgactttt tttcctttgc
      901 cgactttgcg ctcagtgtat cggaagtgaa tcaacaggtg aaagacttgt tggaaagtga
      961 tccggtgctt gaggatattt ctgttaaggg agaaatatcc aatttatcca gaccggtttc
     1021 cggtcatgtt tatttcactt taaaggatga aaaatccagc ttaaaatgtg ttatgtggcg
     1081 gactgttgct gccgaatttc aggaattgtt taaaaacggt gcggctgtga ttgttcatgg
     1141 ccggatcggt gtctatgaaa gagacgggct atatcaactc tacgcggatg ctgtttatcc
     1201 cgtcggtagg gggaaacttt ttgaagagtt cttgagattg aaagaaaagc ttgaaaagga
     1261 aggactgttc gattccggca gaaaaaagcc gctgccttat atgcccgaaa gaatcggtgt
     1321 tgtcacgtcc agcagcggag cagcgatcca ggatatcttc aatacgattc ggaaacggtg
     1381 gcctttggct gaaatatact tgagtcccgc atccgttcaa ggcgaggatg ctcccgcaga
     1441 actgatcaaa gcatataaaa aattggtgga aatgaatccg gatgttattt tgattgccag
     1501 aggcggcggt tctttggagg atctttgggc atttaatgac gagacgctgg tgcgaacgat
     1561 cgcgagcagc aagattcccg ttatcagcgg agtcgggcat gaaacggatt tcacactgct
     1621 cgatttcgcg gctgattttc gcgcgtctac tccgaccgcg gcggctgtcg cggctacacc
     1681 ggatcgattg gaaattatcc aagccgttga ttttctgacg gttaaaatga atcgattgat
     1741 gcgtgaaaag ttggataaga cagcggaaaa aatcaatcaa ttaaaccgac gatttgagct
     1801 ggctgctccg ttgaatcaga ttcgacagga acaaaaaata ttggaacaga tccatattcg
     1861 gctgaatctt tgggctgatc gatattttaa acaacaaagg gaactattaa tcggtttgag
     1921 ccgtcgtttg gaaagtgccg gcccgagttc cgttttaaac cgagggtacg cattaatcga
     1981 agatgaaaac ggaaaaataa ttgtttccgc ctcatgtttc tattcggatg aaaaagtgac
     2041 tattcggatg aaagacggag atagggatgc gatgattttg taaggacggg ataaaattat
     2101 ggaaaattct tcggaaaaat taacatttaa agaagctttc gagcaacttc aaaaaatagt
     2161 ggaacaactc gaaagtgaaa cagttgaatt agaacaagcg atggagttat ataaggcggg
     2221 tatgcaatta tcgaaatatt gcagcagctt attggagcag gctgagttga aaattcggac
     2281 gatcggtcaa accaccgtaa acggagataa agacagtgtt tctgactgaa tttttcggta
     2341 atccggcggt tgtttccgga ttggctgcca tgatgcttgc gcagctatta aaattgttac
     2401 tgaattatgc attaaacaaa aaatgggatt gggttttact gattgagagc ggcggaatgc
     2461 cgagctccca ttccgcaatg gtcacagcca ttgcggtatc gatcggatta tgggaaggat
     2521 ttgataccgc tgcttttgcg gcggctttcg ggttgatggt tattgtgact tatgattcgg
     2581 ccaatgtgcg atggcaatcg gggctgcatg cacagaaaat taatcaatta ttacgggatg
     2641 ttttcagcgg acaaccgatc aatgatcaat tgttgaaaga agtcatcggt catactccgc
     2701 ggcaggttta tataggggtt attcttggaa ttttggtggc gttggcgatc gattctttgt
     2761 gggtatgaag cagacaaaca gaatgaataa aagaattcat gttttaggcg gcgcagcgat
     2821 tatcctcggt gcgattctgg taggattgat tttgtctgtt tttctgtcaa acaaccatcg
     2881 cgttcgtgct tctcaaacaa ccggcttgcc gaccgtaacc ctgatttcgg aaatgattcc
     2941 cacccatacg accgttttgc cgaccgaaac gatgacgccg agtccggttg tcccttctgc
     3001 gataccgacg gttcaaccga ctgcgttgag cgctgcagac tggaaaaact ggccgattct
     3061 tcccgaaagg gtcagcacaa agatgatcga tgtttatcga gcggggcagg aaaatggcaa
     3121 caaagcaaac cggttcagca aagtcggtga cagtaacagc attatgcctt cttttttggg
     3181 atgctttgat tacggtgaaa atgggtataa attaggcaag tatgccgctc tggaagaaac
     3241 gatcaagcaa tttcaatggt ctttttcgcg cgaatcccgg gcgacagcca acggtattac
     3301 ggcgatgcaa ctggatacct atcattggta tgaggatgat gtctgttggc cttatgaatc
     3361 cgcaacgtct tgtgaatacc ggttgtggca accatccatt gcttttatcg cattgggaac
     3421 gaatgatgtc tacatgccgt tagccgaatt tgataagcac atgcgttcgt tggttcaaaa
     3481 atccatcgat cgatatgtgg tgccgatttt agtgacaaaa gcggataatc tggaaggaga
     3541 cggcagtttc aatcaagcaa ttgcacaaat tgccttggat tatgaggtgc ccttgtggaa
     3601 tttatggcgg gcgatggatc cgttacccgg gcatgggctt cgtgaaaatg atgttcatcc
     3661 cacatttaac aatacgagcc tttgtgattt cagcggcgat gatttgcaga cttacggctg
     3721 gacggttcgt aatttaaccg gtttgcaagc tttggatcga gtctggcatt tgctgaatca
     3781 gggtatgaca tcttcctctc agtgaaaaaa gcttatatgt ttcatatctg caaataaaga
     3841 aaaaggttct tgctgaaccg ttttataatt taaaaatcaa tctttggtat attcaacgga
     3901 tgttctgcgc tcggtatccg tggcaaatat gttcg
//
LOCUS       DAOMIV010000155         3925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_24017_length_3925_cov_3.761499, whole genome shotgun sequence.
ACCESSION   DAOMIV010000155 DAOMIV010000000
VERSION     DAOMIV010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24017_length_3925_cov_3.761499"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PKV59_04690"
     CDS             complement(<1..1082)
                     /locus_tag="PKV59_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQP46083.1"
                     /translation="MYKVNINGQDYLSEKDMALMDFLRDVVGITSVKNGCHEGACGAC
                     TVIIDGKTYRSCTQKLSRLEGKKVQTIEGFTQRERDVFSYAFAAVGAVQCGFCIPGMV
                     VCGKNLTDNNPDPTVNDVRLAIRNNICRCTGYAKIVEAILLAAKMLREDLPVPEVKQT
                     GRIGERTMRIDAKIKALGQAKYADDFHFDGMLYGKNVFSKYARAKVLSIDVTPALKLP
                     GVVAVFTAKDIPGHRYIGHLSQDWPGLIDAGEETKFIGDSVAIVVAESLEQAVAGVQA
                     VAVEYEELTPVRNPEEAMAPDAPQIHGEGYEHFGKFIIPKNNLYDHEEVIRGDADAKI
                     ASSRYVAEGTFILPPTEHAFMEPETSV"
     gene            1524..2393
                     /locus_tag="PKV59_04695"
     CDS             1524..2393
                     /locus_tag="PKV59_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HQP46084.1"
                     /translation="MIDWIIRFVKGAIVGIGAILPGLSGGVLTVIFGIYDPLIRFLAD
                     IRKDFVKNVRYFLPIGIGVAVGILLFSTVVSAAFGKYQAQFVCLFIGFVLGTFPSLFK
                     KAGLKGRNGKHYVIMFVTAILLFLLLLSGQQVLTNVQPNFLVWIGSGAIFAFGFIVPG
                     LSPSNFLIYFGLYDKMADGIKNLDFGVLIPLAIGAVLCILLFAKLMAFLFDRYYSGMY
                     HFILGTVIGSSIAIFPAVVFPAFTAEGLAASGLSFIGSLAFCLILLIIGIIISWQFGK
                     LEERVESQKAAEI"
     gene            complement(2514..2900)
                     /locus_tag="PKV59_04700"
     CDS             complement(2514..2900)
                     /locus_tag="PKV59_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46085.1"
                     /translation="MDTKKLIYWMPRLLSVLWILILLLTSSQTIPQNSALSENIKWVI
                     RNNQTAIAVALLLLIGWRYEHVAGVCFILLAVFNSVMVTFTDPGSKWYVIGKMLPVTL
                     PGFIISVLFFIAYAQKVYDEMDNEGK"
     gene            complement(3060..>3925)
                     /locus_tag="PKV59_04705"
     CDS             complement(3060..>3925)
                     /locus_tag="PKV59_04705"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF018165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HQP46086.1"
                     /translation="NLDQISVRIAENYAGSDLPLYGSSLRLPERKAVIELLLDFQKLL
                     FPAYFGDPTLIRLPPEHYTHLLLERIRISLRQQIELAMEESPENSKRAQQICKQLISR
                     LPAIQELLEKDLTATFEGDPAAQTKEEIVFCYPGFFAIFVYRIAHELYLSKVPLIPRM
                     MTEYAHGKTGIDINPGAVIGEYFFIDHGTGIVIGETTIIGDHVRLYQGTTLGALSPAS
                     GQANRGVKRHPTVGNNVIIYSGSTILGGDTIIGNNVIIGGNVFITESVPDNTRVTMKS
                     PELTFREVKDL"
BASE COUNT         1015 a          882 c          866 g         1162 t
ORIGIN      
        1 acagaggttt ccggctccat aaacgcatgt tcggtcggcg gaaggatgaa agtgccttcc
       61 gcaacatatc tggaggatgc gatttttgca tcggcatcac cgcggatcac ctcttcatga
      121 tcatatagat tatttttcgg gatgataaat tttccgaaat gttcatatcc ttcgccgtga
      181 atttgcggag catcgggtgc cattgcttct tcaggattac ggacgggtgt taattcttca
      241 tattctaccg caaccgcctg aacaccagcc accgcttgtt ccaaactctc cgcgaccacg
      301 atcgccaccg aatcgccgat gaattttgtt tcttctccgg cgtcgatcaa accgggccag
      361 tcctgagaaa gatgaccgat ataacggtgc ccgggaatat ctttggcagt aaaaaccgca
      421 accacgccgg gaagtttcaa tgcgggtgta acgtcgatcg aaaggacttt tgcccgcgca
      481 tatttactga aaacgttttt cccgtagagc atgccatcaa aatgaaaatc atcggcgtac
      541 tttgcctgcc ccaaagcttt tattttggcg tcgatacgca tggtccgttc gccaattcga
      601 ccggtttgtt tgacttcggg aacaggcaga tcttcccgaa gcattttggc tgccaacagg
      661 atcgcttcga cgattttcgc atatcccgtg cagcgacaaa tgttattccg aatagccaat
      721 cgcacatcat tgactgttgg gtccggattg ttatctgtca gattcttccc gcaaacgacc
      781 attcccggga tacaaaaacc gcattgaaca gcaccgactg ccgcaaacgc ataggaaaat
      841 acatcccgtt cgcgttgggt aaaaccttcg atcgtctgga cttttttccc ttccaatctg
      901 gaaagttttt gtgtgcaaga acggtatgtc ttcccatcaa taatcacggt acaggcaccg
      961 caggcacctt cgtgacagcc attttttacg gatgtgattc ccactacatc ccgtaaaaaa
     1021 tccattaaag ccatatcttt ttcggataaa taatcctgac cattaatgtt tactttgtac
     1081 attttttccg ctttcaaata actaagtatt tcgattattt gcgcttcata cagatacatc
     1141 cgggatgagc ggttcaggaa acggttggga caattcctac tatacaaact ccggacggga
     1201 aactttccgc ctttttgcct gtcctccgaa gcagcaggag aatcaccaaa aaagagaaga
     1261 aagcttcctt tcattaatcc cgaattccgg gattgattta attattattt gattcgatat
     1321 ttcctcaaat cttctttaat tatacctaaa caccgaactg attcggtatc ccttttacaa
     1381 gaccatgtca ataatttaca aggatatttt ctgatgattc ggaaatgccg gatatcctga
     1441 atcagacgaa tcaatttcct aaatatcatt tcttttgata caattcacac tcgattgaat
     1501 aagaaatact tttggaggcg acgatgatcg attggataat tcgctttgta aaaggcgcaa
     1561 tagtggggat tggggcgatt cttcccggac tatcgggagg agtcttgacg gtcatttttg
     1621 gtatttatga tccgttgatt cgatttctgg cggatattcg taaggatttt gtaaaaaatg
     1681 tccgatattt tctgccgatc ggcatcggcg tcgctgtcgg aattttattg ttttccacgg
     1741 tggtttcggc ggcattcggt aaatatcagg cacaattcgt ctgtcttttt atcggttttg
     1801 tcttgggaac ttttccctcc ctgtttaaaa aagcgggctt gaagggcaga aacggaaagc
     1861 attatgtcat tatgtttgtt accgctattc tcctgttctt attactatta agcgggcagc
     1921 aggtattgac caatgtacaa ccgaatttcc ttgtatggat cggcagcggc gcgatattcg
     1981 catttgggtt tattgtcccg ggattatccc cttccaattt tctgatatat ttcggacttt
     2041 atgacaaaat ggcggacggt attaagaatt tggattttgg cgtgctgatt ccgctggcta
     2101 tcggcgcggt actttgtatt ttattgtttg ccaaactgat ggcattcctt tttgatcgat
     2161 attattcggg aatgtatcac tttattctcg gaacggttat cggatcttcc atagcgatat
     2221 ttccggcggt ggtatttccg gcgtttaccg ctgagggatt agcagccagc ggcctttcgt
     2281 ttatcggttc tttggcattt tgcctgatct tgttgattat cggcattatc atctcctggc
     2341 aattcggcaa actggaagaa agagtcgaga gccaaaaagc ggctgaaatt tagcgaatat
     2401 tttgtttgaa aaagaaagaa ccttgccggt tggaattggc ggcaaggttc ttttctatat
     2461 aaaaattcac cattcgattt ttcggaagag cggattcgaa agagcttttt cggtcacttc
     2521 ccttcgttat ccatttcatc atagactttt tgcgcatagg ctataaaaaa cagcacgctg
     2581 atgatgaaac cgggtaacgt gaccggaagc atttttccga tcacatacca tttgcttccc
     2641 ggatcggtaa acgtaaccat gacactgtta aaaacagcca aaaggataaa gcaaacaccg
     2701 gcgacatgct catatcgcca gccgattaat agcagcaatg ccacagcaat ggcagtttga
     2761 ttatttctga tgacccattt aatattctca gaaagcgcac tattttgagg gattgtttga
     2821 gatgatgtca ataataaaat aagaatccat aagacggata ataaccgcgg catccaataa
     2881 atcagtttct ttgtatccat tttttcaccc ggttcagaat ttcggaataa cctttctgat
     2941 tataagagac aaggaagcgg gatgtcaatt caaaacgacc gattattttg aattgaaaaa
     3001 ggggagtttt ctcccccttt tttcaattcc gtcctcaaaa atatccgaaa ttttcgggct
     3061 tacagatctt tcacttcacg aaaagtcagt tccggtgatt tcatggtcac tcgggtgtta
     3121 tcgggtacag attcggttat aaaaacattc cccccgatta taacgttatt tccgatgatc
     3181 gtatccccgc ccaaaatggt tgaaccggaa tagataatga cattattccc gacggtaggg
     3241 tgacgtttga cacctcgatt ggcttgcccg ctggcgggtg acagcgctcc cagagtcgtc
     3301 ccctgataga gacggacatg atcaccgatt atggtggttt caccgataac gatccccgta
     3361 ccgtgatcga tgaaaaaata ttctccgatg actgcgcccg ggttgatatc gatccccgtt
     3421 ttcccgtggg catattcggt catcatccgc gggatgagcg ggactttgct caaatataat
     3481 tcatgtgcga tccgataaac aaaaatcgca aaaaagcccg ggtagcagaa gacaatttct
     3541 tccttggttt gggcggcggg atcgccttca aaagtagcgg tcaggtcttt ttccagcaat
     3601 tcctgaatcg caggcaggcg gctgatcaat tgtttacaaa tctgttgtgc acgtttggaa
     3661 ttttccggac tttcctccat ggcaagctcg atttgttgcc tgaggctgat ccgaatgcgt
     3721 tccagtaata aatgggtgta gtgctccgga ggtaaccgga tcaaggtggg gtcgccgaaa
     3781 tacgctggga atagtaattt ctgaaaatca agcagcaact caattacggc ttttctttcc
     3841 ggcagccgaa gagaggatcc gtacagaggc aggtccgatc cggcataatt ctccgctatt
     3901 cggacagaaa tttgatccag attgc
//
LOCUS       DAOMIV010000156         3899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_24265_length_3899_cov_3.476847, whole genome shotgun sequence.
ACCESSION   DAOMIV010000156 DAOMIV010000000
VERSION     DAOMIV010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24265_length_3899_cov_3.476847"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="PKV59_04710"
     CDS             complement(<1..435)
                     /locus_tag="PKV59_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46087.1"
                     /translation="MALLYPVLSILESLFAGLLLWKPAESGGISRLRLIFMILIVLIV
                     VVVLSGWLFRLKILQRSEKIGTACFFLSAACFFIAFTTRFYFDNPALDFTVAYLKQAL
                     PFILLTAILSLQTFIGIVYYYSKEKGSVSRAFKQLFWGGRIVW"
     gene            complement(445..1947)
                     /locus_tag="PKV59_04715"
     CDS             complement(445..1947)
                     /locus_tag="PKV59_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46088.1"
                     /translation="MDQTNQTKTIMISADHGLAIVYFLQTDVVKTLLEKGFRVVILTD
                     DGVTEKMRERFQFSNLFFEGLRLKDAKIYFETVDHNQQYWIHFLRWMGGSDQVNIHAI
                     DSHLRQMAFETSRRGKLLMPFIKTYIRKLRKSADARKRLVRKQNRYTTNLYGDLFDRY
                     QPNLVIASTAGWRLDRYLLREAAARGIETAFAVIGWDNPSSYRLPGAPVQWVSCWSEI
                     QKQELTLGADWLPERVHVGGIPTYDGYFRKIWRLSRETYFKYHNLDPDRKLISFACSF
                     VTFSPNYQDIEALVNIISQEKLSYPAQLLIRLHPNHFIPGSLFEEEAERIRRLIKDKP
                     YIHLVEPVALGGDLGFYSGEDMPEKDSMMAWSDVFCTVYSTMVVETAIHDRPIISVCI
                     DAPGGWKKPGKFSLSLTEIGEWPTHERFRAAKAGKVVYNEEQLTEALNFYLAHPEADS
                     AERKQFVSDECTFTDGTAGIRTAEYFAAVAENARRHSELIYHFDPLPPAD"
     gene            complement(2026..2985)
                     /locus_tag="PKV59_04720"
     CDS             complement(2026..2985)
                     /locus_tag="PKV59_04720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HQP46089.1"
                     /translation="MRIGQNPAKFVNNVAKPARVTVAILNYIPFISGYYAELPQVLHA
                     CLDSIAESTADVEYDLMVFDNGSCPEVKDLLRDELEQGRIRYLFLSEKNLGKGGAWNI
                     ILNGAPGEIIAYADNDCLFSPGWLSKSIEILETYPNVGMVTARPFRTNPQFYTATLDW
                     AEKTEDVKLESGQFVPFEVFREFDLSLGQTEEEIRSHYEATCDHLLTYHGVQAIAGAS
                     HWQFTAYKKVLSEFLPFQMDRPMGQVKELDLRMNEKGLLRLMPVQPYVMNMSNTLADA
                     AKEKAQSKKAAGRNRLLDMPPVKKTLLGLYNQIFRWYYDDRQK"
     gene            complement(3064..>3899)
                     /locus_tag="PKV59_04725"
     CDS             complement(3064..>3899)
                     /locus_tag="PKV59_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46090.1"
                     /translation="WKQLGEDVDIVLIGEDIGVQEAAEQLGVRYIAEVKRNESGTPLL
                     SDIFALAKDTSDSPLLAYVNADIILFPDFLEQARRMMEKEKKFLLVGQRWDLDIPRKI
                     DFSNNWQGKLRKELADRGRRHPAGGSDYFIFPRTCFEKIPDFAIGRSGWDNWMFYEAR
                     KQGWKLIDCTKAIDIIHQDHDYAHLPNGQSHYRLPESKHNVELAGGKRTIFTLLDCDW
                     QLESNGNLVRPPRTKKKILREMEIFPLIKLKSRVLGEVGFAVFHPEKAYREFRSRKKK
                     D"
BASE COUNT         1090 a          970 c          774 g         1065 t
ORIGIN      
        1 ccaaacgatt cttccacccc aaaataactg tttgaaagcc cgtgagacgg aacctttttc
       61 cttagagtaa taataaacaa tgccgataaa agtttgtaat gacaaaatgg ccgtcaggag
      121 aataaaaggc aacgcctgct tcaggtaagc gaccgtaaaa tcaagcgctg ggttatcaaa
      181 ataaaatcgg gttgtaaacg cgataaagaa acaagccgca gaaagaaaaa agcaagctgt
      241 gccgattttc tccgatcgct gcaagatttt taatctgaaa agccaacccg ataaaacaac
      301 aacaacgatc agcacaatca gaatcatgaa gatcaaccgc agtcgtgaaa tcccacccga
      361 ttcggcgggt ttccaaagaa gtaaacccgc aaaaagagat tcaagaatcg atagaacggg
      421 atagagcaat gccataatct tgtttcaatc agcgggaggc aacggatcga agtgatagat
      481 cagctccgaa tggcgccgcg cattttccgc cacggcggca aaatattcgg ctgtccgaat
      541 tcccgcggtt ccgtctgtaa aagtacattc atctgaaaca aactgttttc gctccgcgga
      601 atcggcttcc ggatgggcca aataaaagtt cagagcttcc gtgagttgtt cttcgttgta
      661 gacgactttt cccgcttttg cggcacgaaa tcgctcatgg gtcggccatt ctccgatttc
      721 cgttaaggat aaactgaact ttcccggctt tttccaccca cccggtgcgt cgatacacac
      781 actgataatc ggccggtcat gaatagcagt ctcaactacc atcgtcgaat agacagtgca
      841 aaatacatcc gaccaagcca tcatcgaatc tttttcaggc atatcctcgc cggaatagaa
      901 accgagatct ccgcccaacg caaccggttc aaccaaatga atatacggtt tatctttgat
      961 cagacgacga atccgttcgg cttcttcttc gaacagtgaa cccggaataa aatggttggg
     1021 atgcagacga attaataatt gagcgggata agatagtttc tcctgagaaa tgatattcac
     1081 caacgcttca atatcctgat aattcggcga aaaagtcaca aagctgcagg caaacgaaat
     1141 cagtttgcga tccggatcaa ggttgtgata tttgaaatat gtttcacggc tgagccgcca
     1201 tattttgcga aaatatccgt cgtaggtggg aattccaccg acatggactc gttccggcag
     1261 ccaatcggct ccgagtgtca attcttgttt ttgaatttcc gaccaacaac ttacccattg
     1321 cacgggtgct ccaggcaatc gataactgct gggattatcc caaccgatca cagcaaaagc
     1381 cgtttcgatt ccgcgcgccg ccgcctcgcg taaaagataa cgatccaacc gccaacctgc
     1441 cgtactcgct ataaccagat tcggttgata tcggtcaaat aaatccccat aaagattggt
     1501 ggtatagcga ttctgttttc gcaccaatct ctttcgcgcg tctgccgatt ttcgtaattt
     1561 acgaatatag gttttaataa acggcatcag caattttcct cttcgggatg tctcgaatgc
     1621 catctggcgt aaatggctgt caatcgcatg gatattgact tgatcggaac ctcccatcca
     1681 tcttaaaaaa tgaatccagt attgttgatt atgatcaacc gtttcaaaat aaatcttcgc
     1741 atctttgagt cgcaatcctt cgaagaacag gttggaaaat tggaatctct ccctcatttt
     1801 ttccgtcacg ccgtcatccg ttaggataac gacccgaaag cctttctcca ataacgtttt
     1861 gacgacatcc gtttgaagaa aataaacgat cgccagccca tgatcggcac tgatcatgat
     1921 cgtttttgtc tgattcgttt gatccattat ctttcctttt tcccgcattc cgtcgcctat
     1981 cgtcttgaga aacaggtatt ccccttaaaa cttcccatgg caatcttatt tttgtctgtc
     2041 atcatagtac cagcggaaaa tttgattata taatcccaac agagtttttt taaccggagg
     2101 catatccaac aggcgatttc gtccggctgc tttctttgat tgggcctttt ctttagctgc
     2161 atctgccaaa gtattactca tattcatcac atacggctga acaggcatca aacgcaacag
     2221 ccctttctca ttcattctta aatccaactc tttaacctgt cccattggac gatccatttg
     2281 aaacggcaaa aactcagata acaccttttt atatgcagta aactgccaat gcgaggcacc
     2341 ggcgattgct tggacaccgt gataggtcag caaatgatcg caagtggctt cataatgcga
     2401 acgaatttcc tcttccgttt gccccaaact cagatcaaat tcgcggaaca cttcaaaagg
     2461 gacgaattgt ccggattcca attttacatc ttccgtcttt tcagcccaat ccaatgttgc
     2521 ggtatagaat tgagggtttg tacgaaaagg acgggctgtc accattccga cgttcggata
     2581 ggtttcaagg atttcaatcg atttggaaag ccatccgggt gaaaaaagac aatcattatc
     2641 ggcataggcg ataatctctc ccggagcgcc atttaagata atattccaag cccccccttt
     2701 cccgagattt ttttcggata agaaaaggta ccggattctt ccctgttcca attcatcccg
     2761 caataaatct ttaacctcgg ggcagctgcc gttgtcaaaa accatcaaat catattcgac
     2821 gtctgcggta ctttcggcaa tgctgtcaag acaggcatgc aagacttggg gtaattcggc
     2881 ataatagccg ctgataaacg gaatgtaatt caaaatcgcg accgtcaccc gagccggttt
     2941 cgcgacattg ttaacaaatt ttgccggatt ttgaccgatc cgcataattt tcctttttat
     3001 ttcccgttct cgtgaaagcc cgatcagggc acttcacgaa aaatttttta attcgaaaag
     3061 atctcaatct ttcttttttc tcgagcgaaa ttcacgatag gctttttccg gatgaaacac
     3121 ggcaaaaccg acctcgccca atactcttga tttcaatttt atcagtggga agatttccat
     3181 ttcccgtagg atctttttct ttgttcgagg aggacgtacc aaatttccgt tcgactccaa
     3241 ttgccaatca caatccaaaa gcgtgaaaat cgtgcgcttt ccaccggcta attccacgtt
     3301 atgtttcgat tccggcaacc gataatggga ttgaccgtta ggcaaatggg cgtaatcatg
     3361 atcctgatgg atgatatcga tcgcttttgt acagtcgatc agcttccagc cttgtttccg
     3421 tgcttcataa aacatccaat tatcccaacc cgaacgtccg atcgcaaaat ccgggatttt
     3481 ttcgaaacaa gtgcgcggga aaatgaaata atcactgccg ccggccgggt gcctgcgtcc
     3541 gcgatctgcc agttctttcc gaagctttcc ttgccaatta tttgaaaaat caatttttcg
     3601 aggtatatcc aagtcccaac gctgcccgac taaaagaaat ttcttttcct tttccatcat
     3661 tcgtcgagct tgttcgagaa aatccgggaa aagaataata tctgcattca catatgccaa
     3721 tagtggagaa tctgaagtgt ctttagcaag tgcgaaaata tcgctcagaa gtggtgtacc
     3781 ggattcgttt cgttttactt cagcaatata ccgcactccc agctgctcag ccgcttcctg
     3841 aacaccgata tcttcgccga tcaaaacaat gtctacatcc tctcccaatt gtttccacg
//
LOCUS       DAOMIV010000157         3890 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_24347_length_3890_cov_3.407040, whole genome shotgun sequence.
ACCESSION   DAOMIV010000157 DAOMIV010000000
VERSION     DAOMIV010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24347_length_3890_cov_3.407040"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /gene="malQ"
                     /locus_tag="PKV59_04730"
     CDS             <1..930
                     /gene="malQ"
                     /locus_tag="PKV59_04730"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQP46091.1"
                     /translation="YANSKGITIVGDIPIFVSMDSSDVWSNPHLFYLDSEGHPTVVAG
                     VPPDYFSPTGQLWGNPLYKWPEHERTDFDWWVQRIKATLLTADLIRLDHFRGFNGYWE
                     VPSGELTAIKGRWVEGAGSALLKKLKDELGDLPIIAEDLGEITQDVVDLRDSFGLPGM
                     KILQFAFSDDARNLFLPHNYIQNCIAYTGTHDNDTAIGWYQTAPESEKDFARRYLMSN
                     GEDIAWKMVRCVWSSVAVYAIAPLQDILQQGPEARMNFPGKAEGNWCYQFKPSDLTPE
                     LAFRIRELNTIFNRIPEINRIPIQRPIIKYMKP"
     gene            complement(1015..2034)
                     /locus_tag="PKV59_04735"
     CDS             complement(1015..2034)
                     /locus_tag="PKV59_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQP46092.1"
                     /translation="MSQKSRFIVIILIVLLLLLIILIYGVFVNVQSGVGFLFIPSKSY
                     TPTITATQTITPTITFTPSKTLDLNLLKTNAVNTYVAVNLIQTQTQLSIATSTGTPDV
                     SVIRTEAVNRAITELTETEMVVRETEKWMPQINSKGFTEKILGKDQSIMVLIDDVSRS
                     FWIDKYEVTNASYQKCIKDGNCSIIDSNVGEEISQDTFNSLPVVNVSWNHAKDYCEWA
                     GKRLPTKDEWLEAAGVDKDFLYPWGKRVTEGMPVNANEENGKPVAADTYPEGCSFYGV
                     CNLLGNVWEWVASSNPEPDSEQQSIMGGGWRTFSGDLNNRIEGKMNKTESADDVGFRC
                     ASDIE"
     gene            complement(2072..3775)
                     /locus_tag="PKV59_04740"
     CDS             complement(2072..3775)
                     /locus_tag="PKV59_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46093.1"
                     /translation="MERNIPRDASESIDFYLRTIYSVLRSKSDTRISVFEEAHSGMDS
                     MMHPDARSDFPDYNAFIYSILRLPECLIKVQRIILGQNIKMFAEQGYSDIEKWEVVSA
                     RARRRYCLYNNSDTLACFITSRSDIDDLIPEITAFQIEWNKFHYLLMKVPDEYLKNID
                     PKNETEYRFIAEAILSPVEDLNRLYTIWGKKTGTWLLEMKRRVSELRVKLLDSSSIQY
                     SRSANSWMKNILQEMPDIVDRPIYFVSSNTHSITNMISGYALSKESELIDFLKKMDDQ
                     SLFHEWDLISRNKLSAKKENLLYYVLKKYQQTPAGAYTVKEQQEIENGGGIFRISGYN
                     TFDIETQIIELSKIKTENIDPRIKLDNIEKLKKSEAILFNIDYPLGMTAFNVMTKFAE
                     EFEDVRGIYIMGKAASLNGIYGDVIIPTAVHDIHSQNTYLFQNCFTASDIEPWLVYGS
                     ILDNQRAVSVFGTFLQNHRFVETLYQGGYTDIEMEAGPYLSAIYEMVRPDRHPTNEFI
                     TLYNNRFDLGILHYVSDTPMSKGKNLGAGTLSYFGMDSTYATSIAILRRIFTIESHFI
                     K"
BASE COUNT         1160 a          788 c          730 g         1212 t
ORIGIN      
        1 tacgcaaatt caaaaggaat cacgatcgtt ggtgatattc cgatttttgt atccatggac
       61 agttcggatg tgtggagcaa tccccattta ttctatttgg attcagaagg gcatccaacc
      121 gtcgttgccg gagttccacc cgattatttt tcacccactg gacaactttg ggggaatccg
      181 ttatataaat ggcccgaaca tgaaagaacc gattttgatt ggtgggttca gcgcattaaa
      241 gcgacactgc ttaccgcgga tttaatccga ttagatcatt tccgggggtt caacggatat
      301 tgggaagtgc catccggaga attaaccgca attaaaggtc ggtgggttga aggggcggga
      361 tcggctctat taaaaaagtt gaaggatgaa ttgggtgatc ttccgataat cgctgaggat
      421 ctgggagaaa ttacacaaga tgttgtggat ttacgggatt cattcggatt gcccggaatg
      481 aaaattctgc aatttgcttt ctcagatgat gccaggaacc ttttcttgcc gcacaattat
      541 attcaaaact gtattgcata tacgggtaca catgataacg acaccgcaat cggttggtat
      601 caaaccgcgc cggaatccga aaaagacttc gcaagaagat accttatgtc gaacggagaa
      661 gatatcgcat ggaaaatggt tcgctgcgta tggagttcag tcgcggtata tgcgattgct
      721 cctttacaag atattttaca acaagggccg gaagcaagaa tgaacttccc cggtaaagcc
      781 gaaggaaatt ggtgttatca atttaaacca tcggatttaa ctccggaatt ggctttcaga
      841 atcagggaac taaatactat ttttaataga attccggaga ttaatcgaat tcccattcaa
      901 aggccgatca taaaatatat gaaaccgtaa taaaaaaatc gaatcgatag agaggttggg
      961 ggatattatt ctcaaaacct ctctattttt taatcatgcc aaaatttttc aatcttattc
     1021 tatatccgac gcacatctga aaccgacatc atcagctgat tctgttttgt tcattttccc
     1081 ttcaatcctg ttattcagat cgccagaaaa ggtccgccat ccgccgccca taatcgattg
     1141 ctgctcggaa tcaggctccg gatttgacga agcaacccat tcccatacat ttcccaataa
     1201 attacataca ccgtaaaagc tgcacccctc aggataggta tccgcagcta ccggctttcc
     1261 gttttcttcg tttgcgttta ccggcatccc ttccgttact cgttttcccc acggataaag
     1321 gaaatcctta tcgactccgg ctgcttccaa ccattcatct ttagtcggaa gtcttttccc
     1381 tgcccattcg caataatctt ttgcgtgatt ccaagataca ttgacgaccg gtaaagagtt
     1441 aaacgtatct tgagagattt cttctcccac attggaatcg ataattgagc agttaccgtc
     1501 tttaatacat ttctgataag atgcgttggt tacttcatat ttatcaatcc aaaaagatcg
     1561 agaaacatca tcgatgagaa ccattatgct ttgatctttt ccgagaatct tctcggtaaa
     1621 tccttttgaa ttgatttggg gcatccactt ttctgtttcc cttacaacca tttccgtttc
     1681 ggtcaattcg gtgatcgctc tgtttaccgc ttccgttcta ataacagaaa catcgggtgt
     1741 cccggttgat gtggcgatgc ttaattgggt ttgagtttga atcaaattaa cggcgacata
     1801 tgtattcaca gcatttgttt tcagcagatt caaatcgagt gttttactgg gagtaaatgt
     1861 tatagtcgga gtaatggttt gtgtagcggt tatggttggc gtataagact ttgacggtat
     1921 aaaaagaaag cccacaccgg attgaacatt cacaaaaacg ccataaatta aaatgatcaa
     1981 aagcaaaagg agaacaatca aaataataac gatgaatcga cttttttgac tcattaaaag
     2041 aatcctttat tcagattttg gttcaaaaat cctattttat aaaatgactt tcgatagtaa
     2101 atattcgcct gagaatcgcg atagaagttg cataagtcga atccattcca aaataggata
     2161 gtgtaccggc gccgagattt tttcctttac tcatcggagt atctgaaaca taatgcaaaa
     2221 ttcctaaatc aaaacgatta ttatataagg ttataaattc atttgtcggg tgacgatccg
     2281 gccgtaccat ttcataaata gcggaaagat acggtccggc ttccatctca atatccgtat
     2341 atccgccctg atataaagtt tcgacaaaac gatggttttg aagaaaagtt ccgaatacgg
     2401 aaaccgcccg ttgattgtct aagatagatc cgtagaccaa ccaaggttca atatcagaag
     2461 ccgtgaagca attctgaaaa agataggtat tctgcgaatg aatatcatga accgcggtag
     2521 gaatgatgac atctccatag attccattta atgatgcggc ttttcccata atgtagatgc
     2581 cgcggacatc ttcaaattct tccgcaaact ttgtcataac attaaaagcg gtcatgccta
     2641 acggatagtc aatattaaaa agaatagctt cactcttttt caatttttcg atattatcca
     2701 atttaatacg cggatcaata ttctctgttt ttattttcga taattcaatg atttgtgtct
     2761 caatatcaaa agtattgtat ccggatatgc gaaatatccc cccaccgttc tcaatttcct
     2821 gttgttcttt gactgtatat gctcccgcgg gagtttgttg atattttttt agaacataat
     2881 aaagaagatt ctcttttttt gcagaaagtt tatttcggct gattaaatcc cattcatgaa
     2941 aaagggactg gtcgtccatt tttttcagaa aatcaattaa ttcggattct ttggagagag
     3001 catatcccga gatcatattg gtaatactat gcgtgttgct tgagacaaaa tatatcggcc
     3061 gatcaactat atcaggcatt tcttgtaaaa tattcttcat ccaactatta gctgatctcg
     3121 aatattggat agaggaactg tccaataact taactctcag ctcagaaaca cgccttttca
     3181 tctcaagcag ccatgtccct gtctttttcc cccaaattgt atacaaccga ttgagatctt
     3241 ccaccggaga tagaattgct tccgctataa aacgatattc cgtttcgttt ttaggatcaa
     3301 tatttttcaa gtattcatcg ggaaccttca ttaaaagata atgaaactta ttccattcaa
     3361 tttggaaggc ggtaatttcc ggaattagat catcgatatc agatctgctg gtaataaaac
     3421 aagccaatgt atcggaattg ttataaagac aatatcttcg tcttgctctc gcactgacca
     3481 cttcccattt ttcgatatca gaatagcctt gttcagcaaa cattttaata ttttggccga
     3541 gaataatacg ttgaactttt atcaaacatt ccggcagccg aaggatacta tagataaaag
     3601 cattgtaatc cggaaaatcg gatcgagcat cgggatgcat catcgaatcc attccggaat
     3661 gtgcttcttc aaatacgctg attctcgtat cagacttgga acgcaagacg gaataaattg
     3721 tgcgcaaata aaaatcaatt gattctgatg catctcgtgg gatattcctt tccatattcc
     3781 tcctttttcg attcccaaag ctaatatttt aaagactatt aaatcacatt tatataattc
     3841 ttttttactt attttattat atggattttt ttcatgaaga tccgatccga
//
LOCUS       DAOMIV010000158         3866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_24545_length_3866_cov_3.452637, whole genome shotgun sequence.
ACCESSION   DAOMIV010000158 DAOMIV010000000
VERSION     DAOMIV010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24545_length_3866_cov_3.452637"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PKV59_04745"
     CDS             complement(<1..458)
                     /locus_tag="PKV59_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HQP46094.1"
                     /translation="MKEDLLNIDVDEEDDDENYPAIARLIELGRQKSYVTIDDILALF
                     PDAEQDVEQLEEAFSALLSAGIPYSEESNLEEPPEEEIDLAEEENEIIPNISMDDLAN
                     IDTDDTIGLYLKEVSRVPLLTADEEVELAQRIERGRMAREELARVNVTPER"
     gene            complement(692..1540)
                     /locus_tag="PKV59_04750"
     CDS             complement(692..1540)
                     /locus_tag="PKV59_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQP46095.1"
                     /translation="MTRIIGDTTSGLTPEQAKEIGIDYIPQIVIFGDKSYRDDYEIDP
                     VTFVKMLEASKELPKTAAPPPSLYTPIFEKLRENKEDALVLAPSVKVSGTYRSAMTAK
                     NDFPDVDIRVIDTGVIGGPVAMELIEAKKMADQHRSLDEIENYINLLNKNVRFYAVVD
                     TLEYLYKGGRIGGASKLFGDALQIKPILTLKDGQVEAFEKQRTQKKALLRIVDLVQAE
                     CPKGGNHGLISLSLNVNLCQEELSFFRKEFKERFGFENVPVFNAPPAFMVHAGPRVVL
                     VSFFTI"
     gene            complement(1542..2552)
                     /locus_tag="PKV59_04755"
     CDS             complement(1542..2552)
                     /locus_tag="PKV59_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HQP46096.1"
                     /translation="MKFEPINKIPGFKIGHAQDSEALTGCTVILCDEGCVGGVDQRGG
                     SPGTRETDLLRPLHMVETVNAILLTGGSAFGLDAAGGVMKYLEEHHQGVNVGPTVVPI
                     VPTAVLMDLTIGSYSIRPDQAMGYQACLNASADSPKQGNYGAGTGGTVGKILGMDHCM
                     KGGIGHAACELEPGIWVGAIVAVNSLGDVVDPDNRNILAGAQLVDESAADKNNKILFA
                     DTLSILSGKTNQKSLETVPVNNTVIGCVLTNVKLSKDEANKLAQCGQNGLVLSIQPAH
                     TMFDGDTMFAISSQRKNGNIHAVTAFAPIVVAQAIVNAIRFADSVGGIPAARDFLSTE
                     RE"
     gene            2708..3334
                     /locus_tag="PKV59_04760"
     CDS             2708..3334
                     /locus_tag="PKV59_04760"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQP46097.1"
                     /translation="MKNVQKFLEKNVFKQFILLIITAIYILINNNSVQAQIPTLNIPT
                     VTGTDQGVVVTVKNDPANPQINLRAGPGTEYDKVGTMVIGQKAVAKGRTEGGTWLLIE
                     YPGAPGGYAWVYSTYVDYLGDLPIVTIPNTPTPRVTNTIDPTLAAQFIITVEPTRLPT
                     FTQPAPLAIPTFPQSSSTYESSVPMGLIIVSCAILGIIIGLFTLAQKR"
     gene            3364..>3866
                     /gene="ricT"
                     /locus_tag="PKV59_04765"
     CDS             3364..>3866
                     /gene="ricT"
                     /locus_tag="PKV59_04765"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQP46098.1"
                     /translation="MAGNSNPVVVGIRFGKLSKIYNFDATGLDEIHLKDLVVVETCKG
                     KQIGEVVKIYQDYEMKDEPLKKVERIATAGELVSNEFWNLRKKEVLEYCEKRAAELNL
                     KDIRILNAEFNYDGSYMILTYSSPTEEKVDLKSLKYDIQKSFSINNIEIKQIGPRDVA
                     KVIGGMGA"
BASE COUNT         1172 a          813 c          736 g         1145 t
ORIGIN      
        1 cgttccggag tgacatttac tcgggcaagt tcttctcttg ccattctacc gcgctcaata
       61 cgttgggcaa gttcaacttc ctcatccgca gtcaacaatg ggactcggct gacttctttt
      121 aagtaaaggc cgatcgtatc atcggtatct atattggcga gatcatccat tgaaatattc
      181 ggaatgatct cattttcttc ttcggcgagg tcaatttcct cttcgggagg ttcttccaga
      241 ttggactctt ccgagtatgg aattcctgcg ctaagcaatg cggaaaaagc ctcttcaagt
      301 tgttcgacat cttgttcggc atccgggaat aatgcgagta tatcgtctat agtgacgtat
      361 gatttctgcc ggcccagctc aattaatctg gcgatagccg gataattctc atcatcatct
      421 tcttcatcaa catcgatatt gagtaagtct tctttcattt tgttttgata tcctctatca
      481 gtaatgtact ctacacagaa tacacttatt tttaataaaa ttcaatccct aaaatttgat
      541 tttttcacgg tttcccttaa aataatctaa aaatgcgcat agccacttat ttatcatacc
      601 acaatatatc aaacttagaa tatatattct attatatttt cattaaaaaa ctgccgttct
      661 atgacggcag cttgaaaatc actaagaaat attaaatggt aaaaaatgaa accaaaacga
      721 ccctgggacc cgcatgaacc ataaaagcag gaggagcatt aaaaacggga acattctcaa
      781 atccaaaacg ttccttaaat tcttttctga agaaagacaa ttcctcttga cataaattca
      841 catttaaaga aagtgaaatt aaaccatgat tcccaccctt cgggcattcg gcttgaacca
      901 aatcaacaat acgtaaaagg gcttttttct gagtcctttg tttttcaaaa gcttcaactt
      961 gtccgtcttt caaggtgagg atcggtttaa tttgtaaagc gtcgccaaaa agtttagatg
     1021 ctccgccgat tctgcctcct ttataaaggt attcaagcgt atcgacaacc gcataaaaac
     1081 gaacattctt atttaataaa ttgatatagt tttcgatttc atccagcgaa cgatgctgat
     1141 ccgccatttt tttcgcttcg attaattcca tcgccacagg accgccgata acacctgtat
     1201 cgataacacg gatatcgaca tcagggaaat cattcttggc tgtcatcgca cttcgataag
     1261 taccactcac tttaactgat ggagccaata ccaaagcatc ttctttattt tctcggagtt
     1321 tttcaaaaat aggggtataa agcgaaggag gcggtgctgc tgtctttggc agttccttcg
     1381 atgcttccaa catttttaca aatgtgaccg gatcgatttc ataatcgtca cggtaagatt
     1441 tatcaccgaa gataacaatt tgaggaatat aatcgatgcc tatctctttc gcttgttcag
     1501 gagttaatcc tgacgtcgta tcacctataa tgcgtgtcat tttattccct ttcagtagat
     1561 aaaaaatctc tggcagccgg tattccacca actgagtcag caaatcgaat cgcatttact
     1621 atcgcttgcg caacaacgat cggcgcaaat gccgtaacag catgaatgtt tccattcttt
     1681 ctctgtgatg agatagcaaa cattgtatcc ccgtcaaaca tagtatgagc gggttgaata
     1741 ctgagaacca atccattctg tccgcattga gccaatttgt ttgcctcgtc ttttgaaagc
     1801 tttacgtttg tcaaaacaca accgataacg gtattattta ccggaacagt ttctaaagat
     1861 ttttgatttg tttttccgga caagattgac agcgtatccg caaataatat tttgttattt
     1921 ttgtctgctg cggattcgtc gaccagttgg gcaccggcga gaatgttacg attatctgga
     1981 tccactacat cacccaatga attaaccgcg acaatcgctc ctacccaaat ccccggttcg
     2041 agttcgcatg cggcgtggcc gattcctccc ttcatacaat gatccattcc caatattttt
     2101 ccaaccgttc cgccggttcc ggcaccataa ttcccttgtt tgggactatc cgccgaagca
     2161 ttcaaacaag cctgatatcc catcgcttga tcgggtcgga tcgaataact cccgatcgta
     2221 agatccatca gaaccgcagt cggaacgatc ggaacgacag tcggtccgac atttacacct
     2281 tgatgatgct cttcaaggta tttcatgacg cctccggctg catctaaccc aaaagcagag
     2341 cctcccgtaa gtaaaatcgc attaacagtt tcaaccatat ggagggggcg tagtaaatcg
     2401 gtctctcttg tgcccggtga tccgcctcgt tgatcaacgc cgccgacaca accttcatcg
     2461 caaagaataa ccgtgcaacc cgtaagtgct tccgaatctt gtgcgtggcc gatcttgaat
     2521 ccaggaattt tgttaatcgg ttcaaacttc attgaaacct tttcgatgga attaaaattt
     2581 ataaaacatt cctagattat aacaagattt ctaaaaaata agagaaacac ctgaatattt
     2641 tcgaaaagct gttcaaatga aatatagtaa aattaacttc gaaaaaaatt atatgggatt
     2701 ttattttgtg aaaaatgttc aaaaattttt agaaaaaaat gtatttaaac aatttattct
     2761 attaataata acggcaatat atatattaat aaataataac tctgttcaag cccaaattcc
     2821 tacgctgaac attcctacgg taaccggtac cgatcaaggt gtagtcgtta cggttaagaa
     2881 tgatccggcg aatcctcaaa ttaatttaag ggctggtccc ggtacagaat atgataaagt
     2941 cgggacgatg gtgatcggcc aaaaagccgt cgcaaaaggg cgtaccgaag ggggaacttg
     3001 gctgctgata gaatatccgg gagctccggg gggatacgct tgggtttatt cgacttatgt
     3061 tgattatttg ggtgatttgc cgattgtgac catacccaat acgcctactc ctcgtgttac
     3121 aaataccatt gatccgactt tagcggcaca atttatcata acagtggagc cgacccgtct
     3181 tccgactttt acgcaacctg ctcctttagc aattcctact tttccgcaaa gcagcagtac
     3241 ctatgaaagc tccgtcccga tgggattgat tattgtatcc tgcgcgattt taggaattat
     3301 cattggttta ttcactttgg cgcaaaaacg ctgataataa aataagttct tactcgaggt
     3361 gatatggcag gaaatagcaa tccggtagta gtcggcattc gatttggaaa actatcaaaa
     3421 atttacaatt ttgatgcgac cggtttggat gaaatccatc tgaaagatct ggttgtagtt
     3481 gaaacgtgta agggaaaaca aattggtgag gtcgttaaaa tttatcaaga ttatgaaatg
     3541 aaagatgaac ccttaaaaaa agtggaaaga attgcgaccg caggagaatt agtatcgaat
     3601 gaattttgga atttaaggaa aaaagaagta ttggaatatt gcgaaaaaag ggcggcggaa
     3661 ttaaatttaa aagatattcg aatactgaat gctgaattta attatgacgg atcttatatg
     3721 atcttaactt attcaagtcc gacggaagaa aaagtcgatt tgaaatcatt gaaatatgat
     3781 atccaaaaaa gtttctcaat caataatata gaaatcaaac aaattggtcc tcgagatgtc
     3841 gcaaaagtta tcggaggcat gggagc
//
LOCUS       DAOMIV010000159         3847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_24701_length_3847_cov_4.393196, whole genome shotgun sequence.
ACCESSION   DAOMIV010000159 DAOMIV010000000
VERSION     DAOMIV010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24701_length_3847_cov_4.393196"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..725)
                     /locus_tag="PKV59_04770"
     CDS             complement(174..725)
                     /locus_tag="PKV59_04770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQP46099.1"
                     /translation="MSENPYIDPTAHVSAKAKIGKNTRIWINVQIREDAEIGEDCVIG
                     KDVYIDHGVKIGNRVKIQNGVSLFNGVTVEDDVFIGPNAAFTNDYYPRAFNRDWEVSE
                     TLIKKGASIGANATIICRHTLGEYCMIGAGSVITSDVEPYTLVVGNPARAIGKICRCG
                     TRLFNDLVCPKCGFDLKKELELK"
     gene            complement(744..1829)
                     /locus_tag="PKV59_04775"
     CDS             complement(744..1829)
                     /locus_tag="PKV59_04775"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQP46100.1"
                     /translation="MIPIAQPLIGEEEQTAVHEVLESGMLASGNIVRAFEEEFADYLG
                     IEHCITTTSGTTALEVALRALGIGPGDKVLTTPFSFIASTNSIVYTGATPVFADIRPD
                     TFNINPEKIEEALRADSDIKALLIVHLFGQACNMDQIIPLVEKYHLLLIEDCAQSHGA
                     MWKGKPVGTFGNVGCFSFYPTKNMTTGEGGAIVTHDANLAETCRLLINHGMAVRYHHD
                     LIGYNYRMTNIAAAIGRCQLRKLDRFNQIRRLNGAILTEGIHHSDITTPFVQDDAFFV
                     YHQYTLRIKNDKRDQFTDYLNANNIGYGIFYPLSIPEQKCYQRFGFNTVWPVTDLIKN
                     QVVSIPVHPGLSEEDLDMIVQVVNKFQ"
     gene            2014..3420
                     /locus_tag="PKV59_04780"
     CDS             2014..3420
                     /locus_tag="PKV59_04780"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009252629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HQP46101.1"
                     /translation="MTQENQVSLEKITSLSKRRGFVYPTSEIYGGLTSSYDYGPLGAE
                     LLRNIRNLWWREMITCREDMIGIDSQILLHPETWVASGHVSAFNDPLVEDKVTHKRYR
                     ADHLIESWLEKNPQDEEVVVAGMGLTEIADFIEKHKVKTPDGNDVFPPKAFNIMFQTS
                     IGVVEGEKMPVYLRGETAQGIFTNFSNILNSTRVQLPFGVGQIGKSFRNEITTGQFIF
                     RTLEFEQAEIEYFFDPEKTDWHELFQDWQSQMWNFIHGTLGIREDKLRWRRHSDQERS
                     FYSKETYDLEYEFPFGFKELWGIAYRTDYDLKQHMDHSGKDLRYRDPNDPSRVFIPHV
                     IEPAVGVNRILMMALCDAYWEDIANNRIVMRFKPNLAPYKAAVFPLVKNKPEIVGKAR
                     EVFHMLAKKMGTNWDDRGNIGKRYFYQDEIGTPFGITIDYQTLEDDTVTVRDRDTMKQ
                     ERVPIHDLANKLLGIIEE"
     gene            3441..>3847
                     /locus_tag="PKV59_04785"
     CDS             3441..>3847
                     /locus_tag="PKV59_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46102.1"
                     /translation="MKDKKLLRIILYIGLIIATLLAYNSDLFSYYDSYTVNHNFDPKV
                     DSKGNYLLTPVISLKKGVYEINFEGNSNGRGNGFYILSGNQIILQDEFLSGEIKETIS
                     FDVEQPSQQIQIGAVYNPESNGFSINRVAIFSK"
BASE COUNT         1157 a          734 c          794 g         1162 t
ORIGIN      
        1 cccggatatg atcggataat gcaaaattct ttcggatcgg tttatgttcg agtgggcatg
       61 cgagatgcac taaaaaaaga ctgccgatgc gaatttccct gatgattttt tatacggata
      121 aacacttttt cgttcgagaa gaatttatat tacccgcggt ttaaaagaat tttctacttt
      181 agctctaatt ctttttttaa atcaaacccg cattttggac acaccaggtc attaaataga
      241 cgagtcccgc aacggcagat tttaccgatc gctctcgccg gatttccgac aaccaaggta
      301 tatggttcca catcagaggt aattaccgat cctgcgccaa tcatacaata ttcgcctaag
      361 gtatgtcgac aaataattgt cgcattggca cctatagatg ctcccttttt aattagtgtt
      421 tccgaaactt cccaatcacg attaaaagct cgcgggtaat agtcattcgt aaaagctgca
      481 ttaggaccaa taaagacatc gtcctctacc gtaaccccgt tgaatagcga tacgccgttt
      541 tgaattttga cccgatttcc gattttcacg ccatgatcaa tgtaaacatc tttcccgatg
      601 acacaatctt ctccgatttc cgcatcttcg cgaatttgaa cattaatcca tattctcgtg
      661 tttttaccga tttttgcttt cgctgaaaca tgggcagtgg gatcaatata agggttctct
      721 gacattcaaa cgctcctaaa aacttattgg aatttattga caacctgaac aatcatatct
      781 aaatcctctt cggataaacc ggggtgaacc ggtatggaaa ccacttgatt ttttatcaaa
      841 tccgtgacgg gccaaacagt attgaatcca aatcgttgat agcatttttg ttccggaatg
      901 gaaagcggat aaaatattcc gtatccgatg ttatttgcat tcaaataatc cgtaaattga
      961 tcccgcttat cattctttat tcgtaaggta tattggtgat agacgaaaaa agcatcatct
     1021 tgaacaaacg gcgtggttat atccgaatga tgaattcctt cggtcagaat tgcaccattc
     1081 aacctgcgaa tttgattgaa ccggtctaat tttcttaatt gacatcttcc gatcgcggca
     1141 gcaatattgg tcatccgata gttatagccg attaaatcat gatgatatct caccgccata
     1201 ccatgattga ttaataatcg acaggtttca gccagattgg catcatgagt gacaattgct
     1261 ccgccttctc cggtggtcat atttttggtc ggataaaaac taaaacaacc gacattgcca
     1321 aatgttccga ccggctttcc tttccacatt gccccatgag actgagcaca gtcttcgatt
     1381 aataaaagat gatatttttc tacaagcggg ataatttgat ccatattgca agcttgaccg
     1441 aaaagatgaa caatcaacaa cgcttttata tccgaatcag ctcgaagtgc ctcttcgatt
     1501 ttctccggat taatattaaa agtatccggt cggatatcgg caaaaaccgg agtcgctccg
     1561 gtatatacaa tggaattggt agaggcaata aatgaaaaag gcgtggttaa tactttatct
     1621 ccggggccga tgcctaaagc tcgcaaagca acttcaagag cggttgtccc tgaggtagtc
     1681 gtaatgcaat gctcgattcc gagataatcg gcaaattcct cttcgaatgc acgaacaata
     1741 tttcctgatg caagcattcc gctttccaaa acttcatgaa cagctgtttg ttcttcttca
     1801 ccgatcagtg gttgtgcaat tggaatcatt tttttatttc cttggaatag atttcagctt
     1861 tttatcaaaa aaattatatc agcgttctgc ggaatgaaag aatttataaa aagaaatatg
     1921 tagtgaactt attaataatc gatcataata gcaagtttat gatgtcgcta tgtgataaaa
     1981 tttgtgggat aaaattcggc aaaggacttt aagatgactc aagaaaatca ggtttcattg
     2041 gaaaaaatta cttctctttc taaacgacgg ggtttcgttt atccgacttc ggaaatttat
     2101 gggggattga ccagttctta tgattacgga cctttagggg cggaattgtt acgaaatatt
     2161 cgcaatttat ggtggcgaga aatgatcaca tgtcgtgaag acatgatcgg cattgattcg
     2221 caaattctgc tccatccgga gacatgggta gcatcggggc atgtgagtgc ttttaatgat
     2281 ccgttagtcg aagataaagt cacccataaa cgatatcgcg cggatcattt aatcgagagt
     2341 tggttggaaa agaatccaca ggatgaagaa gtagttgttg cgggaatggg tctgactgaa
     2401 atcgcggatt ttatcgaaaa gcataaagtt aaaacgccgg atggaaatga tgtatttccg
     2461 cctaaagcat ttaatattat gtttcaaact tccatcggag tcgttgaagg tgaaaaaatg
     2521 cctgtttatc tccgcggaga aacagcgcaa ggaattttta cgaatttttc gaatattttg
     2581 aattccactc gggttcagct tccgtttgga gtgggacaaa tcgggaagag ttttcgcaac
     2641 gaaattacga ccggacaatt tatttttcga actttagaat ttgaacaggc cgagattgag
     2701 tatttttttg atcccgaaaa aacagattgg catgagctgt ttcaagattg gcaaagccag
     2761 atgtggaatt ttattcatgg gacattgggt atccgggagg ataaattacg ttggcggcgg
     2821 cattctgatc aagaacgttc tttttacagt aaagagacat acgatttgga atatgaattc
     2881 ccctttggtt ttaaagaatt atggggaatt gcctatcgga cagactatga tctgaaacag
     2941 catatggatc actcggggaa agatttacga tatcgtgatc caaatgaccc gagtcgggtt
     3001 tttattccac atgtcattga accggcagtc ggagtcaacc ggattttgat gatggcctta
     3061 tgcgatgctt attgggaaga tatcgcaaat aatcggattg tgatgcgctt caaaccgaat
     3121 ttggctcctt ataaggcagc ggttttcccg ttggtaaaga ataaaccgga aatcgttgga
     3181 aaagcccgcg aggttttcca tatgctggct aagaaaatgg ggaccaactg ggatgaccgg
     3241 ggcaatatcg gtaagcgcta tttttatcag gatgaaattg gcactccctt tggaattacg
     3301 attgactatc aaacattgga agatgatacg gttaccgttc gagatcggga taccatgaag
     3361 caggaacgtg ttccgattca tgaccttgca aacaaattac ttggtatcat tgaagaataa
     3421 tataaataag gaataaataa atgaaggata agaaactttt gcgaatcatc ctatatatag
     3481 gattgattat tgcaaccttg ttagcttata attccgatct gttttcttat tatgattcct
     3541 atacagtaaa tcataatttt gatccgaaag ttgattctaa aggtaactac cttctcacac
     3601 ccgtgatttc actcaaaaaa ggtgtctatg agattaattt tgaggggaat tcaaacggca
     3661 gaggaaatgg tttttatatt ctcagtggta accaaattat tcttcaagat gaatttctga
     3721 gcggcgaaat caaagagaca atttctttcg atgtcgaaca gccttctcaa caaattcaaa
     3781 tcggcgctgt ttataatccc gaatcgaacg gattttctat aaaccgtgtc gcaatttttt
     3841 caaagca
//
LOCUS       DAOMIV010000160         3842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_24740_length_3842_cov_5.007394, whole genome shotgun sequence.
ACCESSION   DAOMIV010000160 DAOMIV010000000
VERSION     DAOMIV010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24740_length_3842_cov_5.007394"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="PKV59_04790"
     CDS             complement(<1..1449)
                     /locus_tag="PKV59_04790"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQP46103.1"
                     /translation="MNIPLSWLKDFVDVDLPLEELARVMTMAGLEVDEIHLMGLPGPE
                     EDRHGFKFTGLSWPEDKFVVAEIREVNKHPDADKLVLCRLEDGTGEHTILTGAPNLYP
                     YVGKGRLEQPIKVAYAREGAILYDGHKPGFELTKLKKTKIRGFETKSMVCSAKELGIS
                     EEHEGIIILEPDAPTGVPLARYMGDAVFVIDILPNMARNACIKGVAREVAAQLNLPLK
                     TPKRTVPPEGRPIDGLASVRIEDPEMNPRFILGYAEGIRIQPSPSWVQRRLSAAGMRP
                     INSVVDATNYVMLELGQPLHSFDYDALVKRNHGQAPAIVTRHAYNGEKLMTLDKTERD
                     LDESMMLVTDQAGALSIGGVMGGLESEIVPETTRVLLESAAWNFINIRKTSSKLRLNT
                     EAGYRNSRGVHPAIAEEAARLCLAYIVEWSGGQVASGLIDNYALPVKDSVNHLSENDI
                     VRLLGVEIPLAIAAEYLSRLEFRCRLSDDGTTL"
     gene            complement(1449..2489)
                     /gene="pheS"
                     /locus_tag="PKV59_04795"
     CDS             complement(1449..2489)
                     /gene="pheS"
                     /locus_tag="PKV59_04795"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQP46104.1"
                     /translation="MAENDLLRQLESFERDSLAALEKITDEADLTKWRVANLGKSSFV
                     MQVFSKLGEAPKEDRPKIGQAANQVRERLETALAEKAEFLNRKSLEQKLTQERLDISL
                     PGRQPRKGRLHPANQNMREILAVLSGMGFEIFRSPEIETDEYNFELLNIPAYHPARDM
                     WDTFYTTDPGVLLRTHTSPGQIHAMRTFAPEPIRVALPGMCFRYEQVSARSEVQFNQI
                     ELLAVGKHITFGDLKGTLQDFAHRMFGENVRTRFRPSYFPFTEPSAEMDVECFLCGGK
                     GCSMCKNSGWLEILGCGMVHPTVLKNGGYDPEIYTGYAAGLGPERITILRHNVEDIRY
                     FWGNDIRFLEQF"
     misc_binding    complement(2530..2770)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2933..3391
                     /locus_tag="PKV59_04800"
     CDS             2933..3391
                     /locus_tag="PKV59_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQP46105.1"
                     /translation="METEKEQIIEKIKILSLQKRNAVFRIAAANGLYPGQLPILEYII
                     DNEGCTQKEIAKKMRVSPASIASSTKRLQRSGLIQKKVDEGNLRQNMLFVTEKGRELA
                     ENCRNELSLFQDAFFDGFSEEELTRLSDILDHINRNISQGAKYHSKSFNH"
     gene            3431..>3842
                     /locus_tag="PKV59_04805"
     CDS             3431..>3842
                     /locus_tag="PKV59_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQP46106.1"
                     /translation="MSLKKLFPYIGQFKKQSRLAPLFVALEVVFDILIPFMMSKIVDV
                     GIASQDVSYIIRMCLLMVGAALLALVCGTLGTRFATLSATGFAMNLRRALFSKIQDFS
                     FYNIDKFSTASLTTRLTTDIQFIQTMYQMLLRMFI"
BASE COUNT         1020 a          977 c          796 g         1049 t
ORIGIN      
        1 cagcgtcgtc ccgtcgtccg ataatcggca ccgaaactca agtcgagata aatattccgc
       61 cgcgatcgct aacggaatct caacgccgag caaacggaca atatcatttt cgcttaaatg
      121 atttaccgaa tccttcacgg gcaaagcata attatcaatt aaaccggaag cgacctgacc
      181 tccgctccac tcgacaatat acgccagaca taatcgagcc gcttcctccg cgatagccgg
      241 atgaacgccg cggctgttgc ggtaaccggc ttcggtgttc aatcgaagtt tagaggatgt
      301 cttccgaata ttgataaaat tccaggcagc cgattccaat aaaacacgcg tggtttccgg
      361 cacaatctcg ctttccagac cacccatgac accgccgatt gaaagggcgc cggcttgatc
      421 ggtgacgagc atcatgcttt catccaaatc tcgttccgtt ttatccaacg tcatcagttt
      481 ttcgccgtta taagcatgtc tggtgacaat cgccggagct tggccatgat tccgttttac
      541 caaggcgtca tagtcgaacg aatgcagcgg ttgtcccaat tccagcatca catagttggt
      601 tgcatccacc acactgttga tcggacgcat cccggctgcg ctgagacgac gctgaaccca
      661 tgacggactg ggttgaatcc gaattccttc cgcatatccc aagataaaac gcggattcat
      721 ttcgggatct tcaattcgga ccgaagcaag cccgtcaatt ggccgtcctt ccggtgggac
      781 agtccgtttg ggagtcttca ggggaagatt gagttgtgcc gccacttctc gcgcgacacc
      841 tttgatgcag gcattccgcg ccatattcgg caaaatatcg atcacaaaaa ccgcatctcc
      901 catgtaccgg gctaagggaa ctccggtggg ggcatccggt tcgaggataa tgattccttc
      961 atgctcttcg gatatgccta attcttttgc ggaacaaacc attgattttg tttcaaatcc
     1021 gcgaattttc gttttcttaa gcttggtcag ttcaaatccc ggtttatgtc catcataaag
     1081 aatcgctcct tcgcgagcat aagcgacttt gatgggctgt tccagccggc cttttccgac
     1141 ataaggatat aagttgggag ccccggtcaa tatcgtatgt tcccctgtcc catcttccaa
     1201 ccggcacaaa accagtttat ccgcatcggg atgcttattg acttcccgaa tctcggccac
     1261 cacaaattta tcttccggcc aggataatcc ggtaaattta aaaccgtgac gatcttcctc
     1321 gggccccggt aaacccatca gatgaatttc atccacctca agccctgcca tcgtcataac
     1381 ccgtgccagt tcttccaaag gaagatccac atcaacaaaa tcttttaacc aagaaagagg
     1441 tatattcatc agaattgctc caggaatcga atatcatttc cccagaaata acggatatct
     1501 tcgacattat gccgaaggat cgtaattctt tcggggccca atccggctgc ataaccggta
     1561 tagatttccg gatcgtaacc gccgttcttc aagacggtcg gatgtaccat gccgcagccc
     1621 aaaatctcca gccaaccgga atttttacac atcgagcagc cttttcctcc gcagaggaaa
     1681 cattcgacat ccatctcggc actgggctcg gtaaacggga aataagaagg gcggaatcgg
     1741 gtccggacat tctctccgaa catacggtga gcgaaatcct gcagggttcc ctttaggtcg
     1801 ccgaaagtga tatgtttccc gaccgccaac agctcgatct gattgaactg gacttccgat
     1861 cgggcactta cctgttcata gcgaaaacac atccccggca acgctactcg gatcggttcc
     1921 ggcgcaaaag ttcgcatggc atgaatttgt cccggagaag tatgcgttcg cagcaaaact
     1981 cccggatcgg tcgtgtagaa ggtatcccac atgtctctgg cgggatgata tgccggaata
     2041 ttcaacaact cgaaattgta ttcatcggtc tcaatctccg gcgaacgaaa gatttcgaat
     2101 cccattccgg aaagaaccgc taaaatttca cgcatattct gattggcggg atgcaatctt
     2161 ccctttcgcg gctgacgccc gggcagggat atatccaaac gttcctgcgt gagtttttgt
     2221 tcaaggcttt tgcgatttaa aaattccgct ttttccgcca atgcggtttc taatctttct
     2281 cgaacctgat tggctgcctg tccgatcttc ggccgatctt ccttgggtgc ttctcccaat
     2341 ttgctgaaaa cttgcatgac aaaggaagat tttcccaaat ttgccactcg ccacttggtc
     2401 agatcggctt cgtcggtaat cttttccagc gcagccaaag aatctctttc aaaagactcc
     2461 aattgccgca ataaatcatt ttccgccata gttcctctca agatctaaaa ataaaaactc
     2521 ccgttcctaa tcagggacgg gagaagtccg cggtaccacc cttattgatt gatatccgat
     2581 tatcaatccg ctcgtttccc gttaacgccg ggcctgcgtt ccgaactact gaatccgtaa
     2641 aaaaggatcg ttcccccgga aagctctggc gtgaacttca atcttcttcc ttccatgaaa
     2701 cagtctcagt ctttgctgtt tcttccctgt cggtttccga aaatctactt ttcgccttca
     2761 tcgcatttct taaacttttc ttattataaa tgcaaaaaaa gacatcttgc ctcgtttttt
     2821 cacaaaagtg atgaatatct ctgtgtcact tttttagcgc gattcttttt caagatacac
     2881 tataataagt aatcgattaa ttgttgcact ttatataaca gtaaagaaaa ttatggaaac
     2941 cgaaaaagaa caaatcatcg aaaaaatcaa aatcctttcg ttacaaaaaa gaaatgccgt
     3001 ttttcgaatc gccgccgcaa acggtctcta tccgggacaa ctcccgatat tggaatatat
     3061 catcgataac gagggatgca cccaaaaaga aatcgcaaaa aaaatgagag tgagtccggc
     3121 ttcgattgca tcttcaacca aaagattaca acgatccgga ttaattcaaa agaaagtgga
     3181 cgaggggaat ttacgtcaaa acatgctatt tgtgaccgaa aaagggcgcg aattggctga
     3241 aaattgccga aatgaattaa gcctttttca ggatgctttt ttcgacggtt tctcggagga
     3301 ggaattaact cgactttccg atattctgga tcacattaac cggaatattt cacaaggcgc
     3361 aaagtatcat tccaaatctt ttaatcatta aagaatcgtt ctttatcgga cattattcga
     3421 aggggaatct ttgtcactaa aaaaattatt tccttacatt gggcagttta aaaaacaatc
     3481 caggcttgcc cctctttttg tcgctttaga agttgttttt gacattttga taccgtttat
     3541 gatgtcaaaa attgttgatg tcggtatagc cagccaggat gtcagctata tcatccggat
     3601 gtgcttatta atggtgggag ccgcgttact cgcactcgtc tgcggcacat tgggaacgcg
     3661 gttcgcaaca ttaagcgcta ccggctttgc catgaacttg cgaagagctt tattttccaa
     3721 gattcaggat ttttcgtttt acaacattga taaattttcg accgcttctt taacaacccg
     3781 gttgacgacg gatattcagt ttattcagac catgtatcaa atgcttcttc gcatgttcat
     3841 cc
//
LOCUS       DAOMIV010000161         3822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_24925_length_3822_cov_5.635254, whole genome shotgun sequence.
ACCESSION   DAOMIV010000161 DAOMIV010000000
VERSION     DAOMIV010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24925_length_3822_cov_5.635254"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..502
                     /locus_tag="PKV59_04810"
     CDS             68..502
                     /locus_tag="PKV59_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HQP46107.1"
                     /translation="MENTVETFHAIGYECPKVGVLACIEKVNPKMPETVDGNALKEMN
                     RRGEISGCIVEGPISYDCAVSREIAELKDFRSPCAGDCDILLAPNIHAGNIMGKMLAV
                     TCGAKLAGFVVGAKCPVVMTSRGSSAEEKYLSIVISALVSQK"
     gene            513..1589
                     /gene="buk"
                     /locus_tag="PKV59_04815"
     CDS             513..1589
                     /gene="buk"
                     /locus_tag="PKV59_04815"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HQP46108.1"
                     /translation="MERLLILNPGSTSTKLAVYDDETPVYTESIVHTPEELAPFDCVA
                     DQYAFRRDLVLRSLEQHRIPLNSLTAIVSRGGLLPPIEAGAYRINEDMVWQLRNRPRN
                     EHASNVGAMIAYDIANRLGIPAFIYDGVTVDELTPINKITGLPSMQRKGMGHNLNTRA
                     AAMRYAREQGKNYRDITVIVAHLGGGISVNLHHNGRIEDFITDEEGPFSPERTGGLPM
                     FDVIRECFEPGATYKGMMKLVKNQGGLMAHLGTTDSQKVEERIAAGDEHAKLIYDAMI
                     LNIAKNIGKIAPGVCGTIDAILLTGGIAHSDYVTEGIKHYVSFLAPVIVYPGEDEMNA
                     LALGGLRVLRGEESAKTFHRVENL"
     gene            complement(1717..2931)
                     /gene="metK"
                     /locus_tag="PKV59_04820"
     CDS             complement(1717..2931)
                     /gene="metK"
                     /locus_tag="PKV59_04820"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQP46109.1"
                     /translation="MSTFMNSPKYLFTSESVTEGHPDKICDQISDAILDACLEQDPYS
                     RVACETSVKTGFVLVYGEITTQAFVNFDELVRKVVLDIGYDSSEKCFDGHTCGILSAI
                     STQSPDIALGVDKAQEAKRGEFGEVDFEEVGAGDQGMMFGYACNETPVYMPMPIYLSH
                     RLTEKLSEVRKNGTLPWVYPDGKSQVTIEYSYGKPKRVDTVVISTQHSASMRDKHDEI
                     EAAVRKYVIDPVLPTELVDRDLKVFVNPTGEFIIGGPQGDSGLTGRKIIVDTYGGMGR
                     HGGGSFSGKDCTKVDRSASYATRWVAKNIVAAGLAERCEIQISYAIGVAKPISINVET
                     FGTGKISDEEIVRLIDRNFDLRPGAIIKRLNLRRPIYRKTSNYGHFGREDADFTWEKL
                     DMAEALRRDAGL"
     regulatory      complement(2995..3098)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3218..>3822)
                     /locus_tag="PKV59_04825"
     CDS             complement(3218..>3822)
                     /locus_tag="PKV59_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HQP46110.1"
                     /translation="GETVAKALRKRFFDAWYFDNKEAAVAQALSLIPEGTSVSWGGSM
                     SIVEVGLKDKIQNGKYKLIDRDTAKTPEERIEIMRQALLADTFLMSANAISEDGQLFN
                     VDGNGNRLAALLYGPKQVIVIAGVNKIAHTAADALERARTIAAPTNISRFDVATPCKI
                     TGTCSDCTTAESQCAQIVQTRICRPAGRIKVILVGTKLGY"
BASE COUNT          964 a          973 c          921 g          964 t
ORIGIN      
        1 ataatacatt ttttctgttg cagatcggga taggtaacca ttccgccgtc caccgaaaaa
       61 atgtattatc gaaaacaccg tcgagacgtt ccatgccatc ggatacgaat gtcccaaagt
      121 gggagtgctg gcttgcatcg agaaagtaaa tcccaaaatg ccggaaacag tagacggaaa
      181 cgcattgaaa gaaatgaatc ggcggggaga aatcagcgga tgtatcgtcg aaggaccaat
      241 ttcctatgat tgtgcggtat cgcgtgagat cgccgagctg aaagattttc gcagcccctg
      301 cgcgggtgat tgcgatattt tgctggctcc caatatccat gccggcaata ttatgggaaa
      361 aatgctggca gtcacctgcg gtgcaaagct ggcgggtttt gtggtcggcg cgaaatgtcc
      421 ggtcgtgatg accagccgcg gttcctcagc ggaagaaaaa tatctgtcga tcgtcatcag
      481 tgctctggtt tcgcaaaaat aggagaaatg ttatggaacg tctgctgatt ctaaaccccg
      541 gatccacctc caccaaattg gctgtatatg atgacgaaac accggtgtat acggaatcca
      601 ttgttcatac gcccgaagaa ttagcccctt ttgattgcgt ggcagatcaa tatgcctttc
      661 gccgtgattt ggtgctgcga tccctcgaac aacaccggat tccgctgaac tcgctgacgg
      721 caatcgtctc tcggggcgga ttgcttcctc ccattgaagc gggagcttat cggatcaacg
      781 aggatatggt ttggcaatta agaaatcgcc cgcgtaacga gcatgcttcc aatgtcggtg
      841 ccatgatcgc ttatgatatc gcaaaccggt tgggaattcc cgcttttatt tatgacgggg
      901 ttacggtgga tgaattgaca cccatcaata agataaccgg tctgccgtcc atgcaacgca
      961 aaggcatggg gcataatctc aatacacgag ctgcagcgat gcgttatgcc cgggaacagg
     1021 gaaaaaacta tcgagatatt accgtcattg tcgcccattt gggaggaggc atttcggtga
     1081 atctgcatca taacggacgg atcgaagatt ttatcacgga cgaagaggga ccgttttctc
     1141 ccgaacgcac cggcggttta ccgatgtttg atgtcattcg ggaatgcttc gaaccgggcg
     1201 caacctacaa aggcatgatg aagttggtga agaatcaagg tggtctgatg gcgcatctgg
     1261 gaacgaccga cagccaaaaa gtggaagagc gaatcgccgc gggggatgaa catgccaagc
     1321 ttatttacga tgccatgatc ctgaacattg ctaaaaatat cggaaaaatc gctcccggcg
     1381 tctgtggaac aatcgacgcc attcttttga cgggcgggat cgctcattcc gattatgtca
     1441 ccgaagggat caaacattat gtttcttttt tagctcccgt aatcgtttat ccgggggaag
     1501 atgaaatgaa cgcgctggct ttgggaggcc tccgagtttt acggggagaa gaaagcgcca
     1561 aaacattcca tcgtgtggaa aacctgtaag gatagagaaa cagcccggca tatagaggaa
     1621 cattcttcgc gatttgcaga tataaaaatc cccatttccg gttggcaaac cgaaaatggg
     1681 gattgctttt taattttgca aaaatcgctt cttgcttcag agtccggcat cgcggcgtaa
     1741 agcttccgcc atatccagtt tctcccaagt gaaatccgca tcttcacgac cgaaatggcc
     1801 gtaattcgaa gtcttgcgat aaatcggacg gcgcagattc agccgtttga taatcgcgcc
     1861 cggccgtaaa tcaaaatttc gatcgatcag ccgcacgatt tcctcgtccg atatcttccc
     1921 cgttccgaag gtttcgacat tgatgctgat cggtttcgcc acaccgatgg cataggaaat
     1981 ctgaatttcg cagcgttccg ccaagccggc tgcgacgata tttttagcca cccaacgggt
     2041 cgcataactg gctgaacgat cgaccttggt acagtcttta ccgctgaacg atccgccgcc
     2101 gtgccgtccc ataccgccgt aagtatccac aatgattttc cggccggtca agcccgaatc
     2161 tccctgcgga ccgccgataa taaattcacc ggtggggttg acaaatacct tcaggtctcg
     2221 gtcgaccaac tctgtcggta ataccgggtc gatcacatat ttgcgaacgg cagcttcgat
     2281 ttcatcgtgc ttatcccgca tggatgcgga atgttgcgta ctgatgacaa ccgtgtccac
     2341 ccgtttcggc tttccgtagg aatattcgat cgtcacctga cttttgccgt cgggatagac
     2401 ccaaggtaaa gtcccgtttt tacggacttc ggagagtttt tcggtcagac gatgggagag
     2461 ataaatcggc ataggcatgt aaaccggtgt ctcattacag gcataaccga acatcatccc
     2521 ctgatcgccg gcgccaacct cttcgaaatc gacttctccg aattctcccc gtttcgcttc
     2581 ctgcgcttta tccactccca gcgcaatatc cggtgattgt gtggaaatcg cagaaagaat
     2641 cccacaagta tgcccatcga agcatttttc actcgaatca tagccgatgt cgagaaccac
     2701 tttgcgaacc agctcatcaa aattgacaaa cgcctgtgtg gtgatttctc cgtaaaccag
     2761 aacaaaaccg gttttgacgg atgtttcaca ggcaacccgt gaatacgggt cttgttccag
     2821 acaagcgtcc aatatcgcat cactgatttg atcgcagatt ttatccggat ggccttctgt
     2881 gacggattcc gaagtaaaaa ggtacttcgg ggaattcata aatgttgaca taacgatatc
     2941 ctccaataaa acacccccga gtttccaggg gggtgcgatt ggtcatcaca gaccctccta
     3001 tcttccagat ttttcattca ctctgccgga gttggcacct tgataatcag gttgccgagg
     3061 tttcattggg ccggtccctc cacctctctg gataagagcg ccattcaggg gcttataaat
     3121 ttttaattct tcctattaga acataaagaa caatgattgt caaacacttt cacaaaaatt
     3181 gctgacagtc tctgacgatt attggaatcc ttgatgatta atatccgagc ttggtaccga
     3241 ccaaaatcac tttgatcctt ccggccggcc ggcagatgcg ggtttggaca atttgagcgc
     3301 actggctctc cgccgtcgta cagtccgaac aagtaccggt gattttgcac ggtgtcgcta
     3361 cgtcaaagcg ggaaatgttc gtcggtgccg caatcgtccg agcccgttcc agcgcatccg
     3421 cagccgtatg agcgatttta ttcacgccgg caatgacaat gacctgcttc ggtccgtaca
     3481 gcaatgcagc gagacggttg ccgttcccat ccacattaaa cagttggccg tcttcactga
     3541 tagcgttggc actcatcaaa aaggtatcgg ccaacagcgc ctgtcgcata atctcaattc
     3601 gttcctcggg agtttttgct gtatcacggt caatcagttt atattttccg ttttggattt
     3661 tatcctttaa acccacttct acgattgaca tcgatccgcc ccaagagaca gaagtccctt
     3721 ccggaatcag gctcagcgct tgagccactg ctgcttcttt gttatcgaaa taccaggcat
     3781 caaagaacct cttgcgcaac gccttggcaa ccgtttcacc ca
//
LOCUS       DAOMIV010000162         3804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25098_length_3804_cov_2.809816, whole genome shotgun sequence.
ACCESSION   DAOMIV010000162 DAOMIV010000000
VERSION     DAOMIV010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25098_length_3804_cov_2.809816"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PKV59_04830"
     CDS             <1..798
                     /locus_tag="PKV59_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46111.1"
                     /translation="DDINEDECRYGDLVAVSWAALQADLKKGNQTLAEKLISENRFTA
                     SDYQKALESANTNAAETVSVSSFETLESVLLNPDVRSVTVDSIGTPLIITGELTIPKG
                     VTVTINRGNDFYIENNLINNGTIEVTGADSVSPNFINYSVMAIQKGGKVTNNGKIVLQ
                     PSVLADTEDRGPVGGQLRIFDGTFENNGSVYLKAGKVNTHGGMVAVIEGPFTNNALII
                     VDGFQIDIAGTFTNNADGIIINNTYISTAKGGTFINNGSLLGEKIVE"
     gene            926..2503
                     /locus_tag="PKV59_04835"
     CDS             926..2503
                     /locus_tag="PKV59_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46112.1"
                     /translation="MKRNKEPVKPNENLKSVLEFLEISNLALAKALDLDPSLISRYLS
                     GERRLFAASAQMNAIADFIIKRCDQLRDIQWLTDQFHSAGLLTDVSATGQFKQNLILW
                     LASDGEILRKNFGLAKPVDIGHFAPREKNRSISTMDEVQIGGLSIVSELFQILQNLPQ
                     KSVIDIFISNDRIGTITNQDISNTLLKQIEKNDLHVRMIVCVSGDTQAMSQILNTYIS
                     ALVSGHIRLSVIFAVTQTVINQMTLLIPDRYVLLITETNGSTAPPVAVPVRNSQFITD
                     MGRNFEMAWHYGQPLLKLYDDNFSRNILEILFTEFCSPGPLDVVKDSINPMYMPVKAY
                     DRFLKTRGHHQDEFSWRSAEFARFKKGMDNVLAADVPFREILSMSRLHDIVSKGSCRM
                     AGLYFMERGYIDLDTEGCIAILNGYIEYLKNVPNFNLVIVDDLNQLHRNNCWQLKQNH
                     HLAINYWNGPEPIMIYSNQIMLLREFQTHFEMLWEQGQKSLPNRANVIAILQNIIDQL
                     IENFGTEQKDNQDSYPI"
     gene            2555..3526
                     /locus_tag="PKV59_04840"
     CDS             2555..3526
                     /locus_tag="PKV59_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46113.1"
                     /translation="MKKHMMVIGLISLFYIFITCGATMHRSFKTQDVSVSAGENFKFG
                     DETYALEKWIKMPKYDSESKTGYGLSFLQKEGVMPVQLSISTNGSPSAYASLLDMKLE
                     IDGENLEPENISFENVKDVPDYTARIIFEFAVPNQSKLPDKGTLFYTNKKIQKEIDLS
                     ALEAPEKNIEISAENPEILNFCGNWKLSSIIFHTPDTGNGIFEIDATLDISDGFEYGR
                     DLTLSKDMSLESDVDIDALIDAISELPFIVNDFNLKDDTGWSYSDGKMKTIPDGTELM
                     ASYNEKDEVLLLVHSGKVDILSQKTDKNAVKSGPVLMEITAVFKKAN"
     gene            3541..>3804
                     /locus_tag="PKV59_04845"
     CDS             3541..>3804
                     /locus_tag="PKV59_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46114.1"
                     /translation="MERKVLCPVCGLSNDFVSPRRMRCRSCGNNFEITENDFQNVKFI
                     EINDGYHSWQSQPTAYHTIYACLSYPEYCDLCIHKNDLFCRNGK"
BASE COUNT         1194 a          776 c          817 g         1017 t
ORIGIN      
        1 gatgatatca atgaggatga atgccgttat ggagaccttg ttgcggtctc atgggctgct
       61 ttgcaagcgg atctcaagaa agggaatcaa acgcttgccg aaaaattaat ctcggaaaac
      121 cgattcaccg caagtgatta tcaaaaagca cttgaatctg cgaatacaaa cgccgcggaa
      181 accgtttctg tctcttcttt tgaaacatta gagtccgtgc tgctaaatcc ggatgtccga
      241 tcggttaccg tcgattcgat cggcacccca ttaataatca ccggcgaatt gacaattccg
      301 aaaggtgtaa cggtgacgat aaaccgggga aatgattttt atatcgaaaa taatttaatc
      361 aacaacggaa caatcgaggt aacgggagca gattccgttt ctccgaattt tataaactat
      421 tccgtgatgg cgatacaaaa gggtggcaag gtaacgaata acggaaaaat agtgctccag
      481 ccttccgtcc tcgcagatac agaagatcgc ggaccggtcg gaggccaatt acgtatcttc
      541 gatggtactt tcgaaaataa cggctctgtt tatctgaaag ccggaaaagt gaacactcac
      601 ggcggaatgg tagcagtcat tgagggaccc tttaccaaca acgcattgat catcgtagac
      661 ggattccaaa tagatatagc tggaacgttt acaaataatg cggatggaat cattatcaac
      721 aatacgtata tttccacggc aaaaggcggc acgttcataa ataatggctc gcttttaggc
      781 gaaaaaatag ttgaataagt aaattcgacc ggtaaccgtc ttcgcaagga aaaaaacgga
      841 atgagttttt ccgttatttc atcgaaagat cagccgaagg tggaaaataa cgatcatggc
      901 accgataaag ggatctaaag aatacgtgaa aagaaataaa gagcctgtaa aaccaaacga
      961 aaatctaaaa tcagtgcttg aatttttgga gatttcaaat ctggcgcttg caaaagcgct
     1021 ggatttggat ccctccctta tcagcagata cctttcggga gagagacgct tgtttgcggc
     1081 aagtgcgcag atgaatgcaa tagccgattt catcattaaa cgctgcgatc agttgcggga
     1141 tatccaatgg cttacggatc aatttcattc ggcgggacta ctcaccgatg tttccgcaac
     1201 cggtcaattc aaacaaaatc ttattttgtg gttagcgtcg gatggtgaga ttctccggaa
     1261 aaattttggc ctcgccaaac cggtcgatat cggacatttc gctccccgag aaaaaaatcg
     1321 aagcatttca acaatggatg aagttcaaat cggcggtctg tcgatcgtat cggaactttt
     1381 tcaaattctt caaaatcttc ctcaaaaatc cgtcatcgat atatttattt ccaatgatcg
     1441 gatcggtacg atcaccaatc aagatatttc aaacacatta ttaaaacaaa tcgaaaaaaa
     1501 tgatttgcat gtccgtatga tcgtttgtgt atccggagat actcaagcaa tgtcccaaat
     1561 tttgaatacc tatatcagcg cgctggtttc gggacatatt cgtttatccg ttatctttgc
     1621 ggtaacgcaa accgtgatca atcaaatgac tctcttgatc cccgatcggt atgttctgtt
     1681 aattaccgaa acaaacggca gcacagcacc gccggttgcc gttcccgtcc gaaattcgca
     1741 atttatcacc gatatgggac gaaactttga aatggcatgg cattacggac agccgttgct
     1801 caaactttat gacgataact tttctcgcaa tattctggaa attcttttta ccgagttttg
     1861 ttctcccggg ccgcttgacg tggtcaagga cagcatcaat cccatgtata tgccggtaaa
     1921 agcttatgac cggttcctga aaacgcgcgg acatcatcag gatgaattct cttggcggag
     1981 cgcggaattt gcccgcttta aaaagggaat ggataatgtg ttagctgcag acgtcccgtt
     2041 tcgagaaatc ttgtcgatgt cccgtctcca tgatatcgtc tcgaaaggtt cctgccgtat
     2101 ggccggcctt tattttatgg aacgcggtta tatcgatctc gatacggaag gttgtatagc
     2161 aattctcaac ggatatatcg aatatctcaa aaatgtgcca aatttcaatc tggtgatcgt
     2221 tgatgatctg aatcaacttc atcgcaacaa ttgttggcag ctgaagcaga atcaccacct
     2281 tgcaatcaat tattggaacg gtccggaacc gataatgatc tattccaatc agattatgct
     2341 cctgcgggaa ttccagactc attttgagat gctttgggaa caggggcaaa aatccttacc
     2401 gaaccgggca aatgtaatcg ctattttaca gaacattatc gaccagttga ttgaaaattt
     2461 tggaacagag caaaaagaca atcaggattc atatccaata taaatttcat cttttcaacg
     2521 gatgaaaatc gatataaaca aaaaggaaca aaaaatgaaa aagcatatga tggttatagg
     2581 gttaatcagt cttttctata ttttcataac ctgtggtgcg accatgcatc gatcttttaa
     2641 aactcaggat gtttcagtct ccgccggtga gaatttcaaa ttcggggatg aaacctatgc
     2701 cctcgaaaaa tggataaaaa tgccgaaata tgactcggaa tcaaaaacag ggtacggcct
     2761 cagctttttg caaaaagaag gggtgatgcc ggtccaatta tccatttcga caaacggttc
     2821 accgagcgct tatgcaagcc tgcttgatat gaaattggaa atcgacgggg aaaatcttga
     2881 acccgaaaat atcagtttcg aaaatgtcaa agacgtcccc gattatacag cccgcattat
     2941 ttttgaattt gcggtaccga atcaatcaaa attacccgac aaagggacac ttttttatac
     3001 aaataaaaaa atacaaaaag aaattgatct ttccgcgttg gaagcaccag agaaaaatat
     3061 tgaaatatcc gccgaaaatc cggaaatatt gaatttttgc ggaaattgga aattatcatc
     3121 gattattttt cacactcccg acacagggaa tggcatcttt gaaatcgacg caacattgga
     3181 catttcggac ggttttgaat atgggcgcga tttgacactc tcgaaggaca tgtcgttaga
     3241 atcagatgtc gatatagacg cactgattga tgcgatttcc gagttgcctt ttattgtgaa
     3301 tgatttcaat ctgaaggatg ataccggctg gtcgtattcc gatggaaaaa tgaagacaat
     3361 tccggacgga actgagttaa tggcgtctta caacgagaag gatgaagttc ttcttttggt
     3421 gcacagcggc aaagttgaca ttttatccca aaaaaccgac aaaaacgctg ttaaatccgg
     3481 cccggtatta atggagataa cagcggtttt taaaaaagca aattagagaa tggggatgaa
     3541 atggaaagaa aagtgctttg ccctgtttgc ggattaagca acgacttcgt ttctccaagg
     3601 aggatgcgat gccggagttg cggaaacaat tttgaaatta ccgaaaatga ttttcagaat
     3661 gtgaaattca tcgaaataaa cgacggttat cacagttggc aatcccaacc cacagcttat
     3721 catacgatct atgcctgttt aagttatccc gaatattgcg atctctgtat ccataagaat
     3781 gacctttttt gccgaaacgg caaa
//
LOCUS       DAOMIV010000163         3791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25217_length_3791_cov_3.563437, whole genome shotgun sequence.
ACCESSION   DAOMIV010000163 DAOMIV010000000
VERSION     DAOMIV010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25217_length_3791_cov_3.563437"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PKV59_04850"
     CDS             <1..939
                     /locus_tag="PKV59_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQP46115.1"
                     /translation="VSPACDLAKMGYEVTIFEALHLPGGVLVYGIPEFRLPKSVVQKE
                     IDKLKDLGVKIILNAVAGRSFTIDELFEHGFEAVFIGSGAGSPKFLNIPGENLSGVYS
                     ANEFLTRINLMKSYQKDSATPIVNSKKVAVVGGGNVAMDAARCAKRLGAEEVYIVYRR
                     SEDELPARKEEVEHAKEEGIIFRLLNNPVEVIGNEKRFVTGLKCVKMELGEPDASGRR
                     KPIEKPGTEFVLDVDTVIIAIGTSPNPLIKMTTEGLKTNDHGGIIIDEETGATSREGV
                     FAGGDAVTGAATVILAMGAGKKAAAGIDKFIREKKS"
     gene            1210..2430
                     /locus_tag="PKV59_04855"
     CDS             1210..2430
                     /locus_tag="PKV59_04855"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQP46116.1"
                     /translation="MSNQFQIRVHSGSLRGQAFVINKQEFKIGRDPSCDIVLTESSVS
                     RVHLLIYPQPYDAIVLVDNNSTNGTTVNNVQVTAPVQIAENDVIVLGGEVALVLEKVP
                     SMQNAYPPYQTPNYNPAYPNAPQQQIPSYQPPAWDTNYNPSFQQQNQNPYVPVNPVNI
                     PVSGIPNQEINMNRNDHEMESLNSEEQVNNDSDHIPSSPKNEENAESVKQYGFNPYES
                     QQRNSFYPNPNMPFQPNSPQPQSPYPGQVPPAYYPPYPTNYENRPQDPYISQQGYNPQ
                     SGQYNPQASQYPSQYPPSQQYPPYPAPYGADSYQQQPYPYQGYPQTPSQQYPAGYYQQ
                     QPYGGYGPAAEYGQADNLQDENAKKKKLYIILGIVLVLILAIIGFIIYIDSNYLWCDV
                     FPFLWSPEACAIYP"
     gene            2680..3024
                     /locus_tag="PKV59_04860"
     CDS             2680..3024
                     /locus_tag="PKV59_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46117.1"
                     /translation="MNSIDLNPEIISQSDNFIAWKVDEPDGETTYHLDINNLTVHFFV
                     EEWEQFIEFKNLFINTEKDKTGELAQTEDYYLSCEKTENGDLVYSMEIIGATLYFFED
                     DWTEFKELVRNL"
     gene            complement(3033..>3791)
                     /locus_tag="PKV59_04865"
     CDS             complement(3033..>3791)
                     /locus_tag="PKV59_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQP46118.1"
                     /translation="VVMLNFWAQWSKPSLEYKKILESIVIHSIGQIRLAHVNVDTSPL
                     LSSKFSVNTLPTIKIILMGNIAGELIGYQPEFRIMSLLNSIQIPDPIQLNIEKANALL
                     KEGDYQSAADLFREIIELRGQDPEAQYGLAVADIHLGKAIDAYYLLKDFPASKRYNDA
                     EKLLPLAKALSQYDEGKLPENSDSDFAFVAAMRFVMANKYYQAIDGLLEIIKQDRNYG
                     NGIAHKAVLALIELLNDDDPQKNLYRSELASILF"
BASE COUNT         1219 a          716 c          733 g         1123 t
ORIGIN      
        1 gtaagcccgg cctgtgacct tgcgaaaatg ggatatgaag taacgatatt tgaagcgctt
       61 caccttccgg gaggtgtctt ggtctatgga atacctgaat ttcgtcttcc gaaatcagtc
      121 gttcaaaaag aaattgataa attaaaagat ctgggagtaa aaattatttt gaacgctgtc
      181 gccggccgat cgtttacgat tgacgaatta tttgaacatg gttttgaagc agttttcatc
      241 ggttcgggtg ccgggtcgcc aaaattcttg aatattccgg gcgaaaattt gagcggtgtc
      301 tattccgcca atgaatttct tacccgaatc aacttaatga aatcttatca aaaagacagt
      361 gccacaccga ttgtaaattc caaaaaagtt gcggtggtag gcggaggaaa tgtcgcgatg
      421 gacgccgctc gctgtgcaaa aagattggga gcagaagaag tctatattgt atatcgtcgt
      481 tccgaagatg aattacccgc ccggaaggaa gaagttgaac atgcaaaaga ggaaggaata
      541 atctttagat tactgaataa tccggttgaa gtgatcggta atgaaaaaag atttgtcacc
      601 ggtttgaaat gcgtcaaaat ggaattgggt gagccagatg cttccggaag aaggaaaccg
      661 atcgaaaaac cgggaacgga attcgttctc gatgtggata cggtaattat tgcaatcgga
      721 acttccccga atccattaat caaaatgacc accgaagggc tcaaaacaaa tgatcacggg
      781 ggaattatta tagacgaaga aaccggtgcc acatctcgcg aaggagtttt tgccggcgga
      841 gacgccgtta cgggagctgc taccgttatt ttagcaatgg gtgccggaaa gaaagcagcc
      901 gccggaatcg ataaatttat cagagaaaag aagtcttaat cattcttctc tatatgctgt
      961 tccgaggaat attaaaatgc agccttccaa agaagctgca ttttattttg ctttttggca
     1021 aatattttgt gattgataat ttgattaagg aaaaacatgg tacgatattt tttaaatgga
     1081 atttatcatt cttcaatcta tcacttatat tgcattagaa tctttacaaa aatcgttatg
     1141 ttataattgc tcgaaataac tacaaagatt tgtagtgaat gacggaatga aaatgggagt
     1201 aagaaattta tgagtaatca attccaaatc cgtgtacact ctggctctct gcgcggtcaa
     1261 gcttttgtca tcaataagca agaatttaaa atcggaagag atccttcttg cgacattgtt
     1321 ttgaccgaga gttctgtttc aagagtccat ttattgattt atccgcaacc ttacgatgca
     1381 attgtgttag ttgacaataa ttcaaccaac ggaacaactg ttaacaatgt gcaggtcaca
     1441 gcccctgttc agatcgctga aaatgatgta atcgttttag gaggagaagt cgctttggtt
     1501 ttggagaaag tgccttcgat gcaaaacgct tatcctccgt accaaacgcc gaattataat
     1561 ccggcatatc ccaacgcacc ccaacaacag attccgtcat atcaacctcc tgcctgggat
     1621 acaaattaca atccatcttt ccagcaacaa aatcagaatc catatgttcc ggtcaatcct
     1681 gtaaatatac ctgtttccgg aattcctaac caagaaataa atatgaatcg caatgatcat
     1741 gagatggaga gtttaaactc agaagaacaa gttaacaatg attctgatca tattccgtct
     1801 tctccgaaaa atgaagaaaa tgcggaatct gtcaaacaat atggattcaa tccttatgaa
     1861 tcgcaacaaa ggaattcatt ttatccaaat ccgaacatgc cttttcaacc caattcaccg
     1921 caacctcaat ctccttatcc gggacaggta cctccggctt attatccgcc atatccgaca
     1981 aattacgaaa atagaccaca ggatccatat atttctcaac agggatataa tccccaatct
     2041 gggcaatata acccccaggc atcacaatat ccatcacaat atcctccgtc tcaacaatat
     2101 ccgccgtatc cggctcctta tggcgctgat tcatatcaac aacagccata tccttatcag
     2161 ggctatccgc aaacgccgtc acaacaatac cctgcgggat attatcagca acaaccatat
     2221 ggaggttatg gaccggcagc ggaatatgga caagccgata atttacagga tgaaaatgct
     2281 aaaaagaaga agttatacat tatcttaggc attgtattag ttttaatctt agcgattatc
     2341 ggtttcataa tttacatcga cagtaattat ctctggtgtg atgtatttcc gtttttgtgg
     2401 agtccggagg cttgtgcaat ttatccttaa tagtcgataa tcgaatttga aaaactctct
     2461 tgtatgaacc aagcttcaaa cagagagttt ttcaagagga aattttgttt ctcaaaatga
     2521 gctaaaaatt tgaatggtaa atattttgga tgtaagtgaa atcccatcca ttatttgtaa
     2581 tattttcgtt ggcaagtcga atatcaaaca aaagttaatt tataagtgct ttttcggata
     2641 attatttagt caaattacaa gaacagataa agaggtatta tgaattcgat agatttaaat
     2701 cctgaaatta tttcacagag tgataatttt attgcatgga aagtcgatga accggatggt
     2761 gaaacaactt atcatctgga tataaataat ctgactgttc atttttttgt cgaagaatgg
     2821 gaacaattca tcgaatttaa aaatcttttt attaatactg aaaaggataa aacgggcgaa
     2881 ttggctcaaa cggaagatta ttatctcagt tgcgaaaaaa cggaaaacgg agatttagtt
     2941 tacagtatgg agataatcgg agccacactc tatttctttg aagatgattg gacagaattt
     3001 aaagaactcg ttcgaaattt ataaattcat tttcagaaca ggattgaagc cagttctgaa
     3061 cgatataggt ttttttgcgg atcgtcatca ttaagcaatt cgatcaatgc aagcactgct
     3121 ttatgagcaa tgccgtttcc gtaattgcga tcctgtttaa taatctcaag taaaccgtca
     3181 atcgcttgat agtatttatt tgccatcaca aaacgcatcg cagcaacaaa agcaaaatcc
     3241 gaatcagaat tttcaggaag ttttccttcg tcatattggg ataatgcctt tgctaaagga
     3301 agtaattttt cggcgtcatt gtacctttta cttgctggaa aatccttcaa aagataatat
     3361 gcatcaatag cttttcctaa atgaatatct gccactgcca atccatactg tgcttccgga
     3421 tcttgacctc ttaattcgat aatttcacgg aataaatccg cggctgattg ataatcgcct
     3481 tcttttaata atgcattggc tttttcaata tttaattgaa tgggatccgg aatttgtatt
     3541 gaattcaaca gactcatgat ccggaattcc ggttgatagc ctattaattc tcccgcaata
     3601 tttcccatta gaattatttt gattgtcggc aaggtattga cagagaattt ggatgagagt
     3661 aatgggctgg tatctacatt tacatgagct aatctgattt gccctattga atgaatgaca
     3721 atcgattcca atattttctt atattccaaa ctcggttttg accactgggc ccaaaaatta
     3781 agcataacga c
//
LOCUS       DAOMIV010000164         3786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25272_length_3786_cov_4.262396, whole genome shotgun sequence.
ACCESSION   DAOMIV010000164 DAOMIV010000000
VERSION     DAOMIV010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25272_length_3786_cov_4.262396"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            412..1422
                     /locus_tag="PKV59_04870"
     CDS             412..1422
                     /locus_tag="PKV59_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQP46119.1"
                     /translation="MKKKIQIVIPMAGYGSRLRPLTWSKPKPLINLAGRTSLDYLLDE
                     FTSLENEFDPEYIFIISPNGWQIKAYMDKHYPNLKCNYVVQEEMKGQSHAIFLAKDLI
                     SGPMIMTFSDTLLEGDLGFLADEDADGIAWVQWNPEPQRFGVAITDENNRIERFIEKP
                     STDEYKMVIIGFYYFKEGQKLIEAIEQQIKENISLKNEYYIADAINIMIQKGAKFTIH
                     ETKLWLDTGVPETVFSTNRHFLDHGHDNTETAMKRKGVAVVPPVFIAEDATVESSVIG
                     PYVSIASGCHIQNSVIENSIIAENTTITGEVIKDSLIGEKVSLKSTPTAYFIGDNSII
                     EK"
     gene            1475..2623
                     /locus_tag="PKV59_04875"
     CDS             1475..2623
                     /locus_tag="PKV59_04875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQP46120.1"
                     /translation="MYNNLSNRAKTIKPSAIRKYFAVPDDVISLGIGEPDFDSPACVI
                     EAGVHALQSGKTHYTANSGLTELREAISDDLYRRYRVRYDPNSEIIATVGCSEALFLA
                     LAALIDPGDEVIVITPCFVAYQASVLLASGVVVEVPCKFENNFDLDIAAVEAAITPKT
                     KAILLGFPCNPTGAVASREELQKLCDIAVAHNLAIVSDELYDQLVYGIEHVCVPSLNG
                     GFERTMLVGGFSKNYAMTGFRIGYLVAPAPLMDGAYKIHQYLIMSAPTIAQYGALAAI
                     EHGQEDAKRMHDEYDRRRHLVVDGLRSIGLPTVEPKGAFYVFPQISGTGLSSEDFANR
                     LLEKEKVAVIPGNGFGAGGEGFVRISYATSYEKIEQALERMGRFVKSL"
     gene            2795..3760
                     /locus_tag="PKV59_04880"
     CDS             2795..3760
                     /locus_tag="PKV59_04880"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46121.1"
                     /translation="MYGYEYTNHLILNEQMVNLWQEEDGKKVAEVWVLPGYGANLCRF
                     KVDNIDYLYPAPQKLVDIRHYGTPVLYPTPGIVRGEKFTFDGSLYRFPANRGSIFRHG
                     FAMGQKFLFSEPIVSEDGVTLKTSLGIFRDHPLYYLFPIANRLDIDFTLKNRSVRIDI
                     TVTDLDTQKRFPFGFGLHPYFNLIGPREENLIQVPVKKWLDQAENQLIDPNDSPIDFR
                     SPTTVPETAIDEVWTGLTSEASPSIIYQSVGKRLVLHATDDFTHFVTYAPENAPYVCL
                     ENWTCSPDAHNLYAGEKNDVAHLLILNPGEVFTGSVQFSIEPLNN"
BASE COUNT         1048 a          807 c          849 g         1082 t
ORIGIN      
        1 cctcacccga ttgctacctg ttgttttttc ccaaaataaa aaaaatatga tctatttcct
       61 atttaagata aattcgagat gatttccgct ttttccataa attttcatac aaaacacaaa
      121 tcccgacgat ttttttgtta ttcggaaccg gtttattctt gttttttaac cgattcggac
      181 ctgcatttaa acggcttatg cataatctgc tctttttcag aatagcaaat cgaaaaacag
      241 gtttgaacgc aaccgtgcat tttacgtttt tttgttgatg gtaaataccg aatattaaga
      301 aattctgttt ggattgggta taattcgttt attcttaata cgtcattcat atgaaaacgg
      361 atcgaagtcc gagcgataaa ggacaaaaat atctttaaac agggagctga aatgaaaaaa
      421 aagatacaaa ttgtaattcc gatggcagga tatggttccc gacttcgccc gctgacctgg
      481 agcaaaccca aaccattaat caacctggcc ggacgaactt ctttggatta tcttttggat
      541 gaatttacca gtcttgaaaa tgagtttgat cccgaatata tctttatcat ttcaccgaac
      601 ggttggcaaa ttaaagccta catggacaaa cattatccga acctgaaatg caattatgtg
      661 gttcaggaag agatgaaagg tcaatcgcat gctattttcc tggcaaagga tttgatcagc
      721 ggcccgatga ttatgacttt ttcggataca ttgcttgaag gcgatctcgg ctttcttgct
      781 gatgaagacg cggatggaat tgcttgggtt caatggaatc ccgagcctca gcggtttggc
      841 gtagccatta ccgatgaaaa taaccgcatc gaacgtttca ttgaaaagcc ctctaccgat
      901 gaatataaaa tggttatcat cggtttttat tatttcaagg aagggcaaaa attgatcgaa
      961 gcgatcgaac aacaaatcaa agagaacatt tcccttaaaa atgaatatta catagccgat
     1021 gcgattaata tcatgatcca aaaaggggcc aagtttacga tccatgagac caaactctgg
     1081 ctggatacgg gtgtaccgga aactgtcttc tccacaaacc gccatttctt ggatcacggg
     1141 cacgataata ccgaaacagc catgaaacgc aagggggttg ccgtcgttcc tccggtattt
     1201 atcgcagagg atgcgaccgt cgaaagttct gtgatcggcc catatgtttc gattgcttcc
     1261 ggatgccata ttcaaaacag tgtgattgaa aattcgatca ttgcggagaa tacgacgatc
     1321 accggtgaag ttataaagga ttcgttaatc ggtgaaaaag tcagtttgaa gagtaccccc
     1381 actgcttatt ttatcggaga taactccatc atcgagaaat aacatttaat atcctatttt
     1441 ctgtcccaca tttcatatcc cggagggatc caaaatgtac aacaatttat ccaatcgagc
     1501 caaaacgatc aagccgtccg ccatccgtaa atattttgcg gttccggacg atgtaatttc
     1561 cttgggaatc ggcgaaccgg atttcgattc tcccgcctgt gtcatcgaag ccggtgttca
     1621 cgcccttcaa tccgggaaaa cgcattacac agctaattcc ggcctcaccg aattgaggga
     1681 agcgattagt gacgacttat atcggcgcta ccgtgtgcgt tatgatccga acagtgaaat
     1741 tatagccact gtcggatgtt ccgaagctct ttttctggca ctggcagctc taatcgatcc
     1801 gggagatgaa gtgatcgtca tcactccctg ttttgttgct tatcaggctt ccgtgctgtt
     1861 ggcttcgggt gttgtcgttg aggttccttg taaattcgaa aataattttg acttggatat
     1921 tgcggcggta gaagctgcga tcacccctaa aacgaaagcg attttattgg gattcccttg
     1981 caatccgacg ggtgcggtcg cctcgcggga agaattgcag aaactttgtg atattgcggt
     2041 cgcacataat ctcgccattg tgtcggatga actatatgat caattggttt atgggataga
     2101 gcatgtttgt gtaccctcat tgaacggcgg ttttgagcga acgatgctgg tgggaggttt
     2161 ttccaagaat tatgcaatga ccgggtttcg aatcggttat ttggtggcgc ccgctccgct
     2221 gatggacggc gcttataaaa ttcaccagta tttgattatg tcggccccga cgattgcaca
     2281 atatggagct ctggctgcga ttgaacacgg acaagaagat gcaaaacgca tgcacgatga
     2341 atatgatcgc cggcgtcact tggttgtcga tggattgcgc agcatcggtt tgccgaccgt
     2401 tgagccgaaa ggggcttttt acgtctttcc gcaaatttcc ggaaccggtt tatcgagcga
     2461 agattttgcc aaccgattat tggaaaaaga gaaagtggcg gttatccccg gcaacggctt
     2521 cggagcggga ggagaaggtt ttgtccgtat ttcctatgct acttcctatg aaaaaatcga
     2581 acaggcactg gaaagaatgg gtcgttttgt aaaatcgctg taatattttc tatagcgctc
     2641 cttttgcgga cggtttccga tggatatcct catatcgatc gaaaactgtc cgtttttctt
     2701 taacaagttc catcaagctt tttaaaacgg ttctttgtca ttcaaacaac cggaattgaa
     2761 tagactactg ataaaattga gggtcggggg tatcatgtac ggttatgaat atacaaatca
     2821 tttgatctta aacgaacaga tggtaaacct atggcaggaa gaggacggga agaaagtcgc
     2881 ggaagtctgg gttcttcccg gatacggagc aaatctctgc cgttttaaag ttgacaatat
     2941 tgactatctt tatcccgctc cgcaaaaatt agtagacatc cggcattacg ggacgccggt
     3001 actttacccg acacccggga ttgtccgcgg agaaaaattt acatttgacg gatcgctata
     3061 ccggtttccg gcaaatcggg gaagtatctt tcgccatggt tttgccatgg ggcaaaaatt
     3121 tctattttcg gagccaatcg tcagcgaaga cggagtcacg ctgaaaacaa gtctcggaat
     3181 atttcgtgat catccgcttt attatctttt tccaatcgcc aaccgactgg atatcgattt
     3241 taccttgaaa aatcgctccg tcagaatcga tatcacggtg actgatttgg atacacagaa
     3301 acggttccct ttcgggttcg gtttgcatcc ttattttaat ctgatcggtc cgcgggaaga
     3361 aaacctgatt caggtaccgg taaaaaaatg gttggatcag gcggaaaatc aattaatcga
     3421 tccgaatgac agcccgattg attttcgctc tccgacaact gttccggaaa cagccatcga
     3481 tgaggtttgg acgggtttaa cttcggaagc ttcgccatcg ataatttatc aatccgtcgg
     3541 gaagcgtttg gttcttcatg caaccgacga ctttacgcat tttgtcactt acgcacccga
     3601 aaacgctcct tatgtttgtc tggaaaactg gacctgctct ccggatgcgc ataatttata
     3661 tgccggtgaa aaaaacgacg tcgctcactt actgattctg aatccgggcg aagtatttac
     3721 cggaagtgta caattttcaa tcgaaccgct gaataattaa aaacctattt tccggcaaaa
     3781 atatcc
//
LOCUS       DAOMIV010000165         3771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25422_length_3771_cov_3.652583, whole genome shotgun sequence.
ACCESSION   DAOMIV010000165 DAOMIV010000000
VERSION     DAOMIV010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25422_length_3771_cov_3.652583"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKV59_04885"
     CDS             <1..193
                     /locus_tag="PKV59_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46122.1"
                     /translation="NLRRRFRTQNMLKSSVMKSKTNAVPIRRVITFRPNVPVSKAKIT
                     EKDNDYWWIRSFFPFVTRK"
     gene            235..>1124
                     /gene="pepT"
                     /locus_tag="PKV59_04890"
     CDS             235..>1124
                     /gene="pepT"
                     /locus_tag="PKV59_04890"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: protein motif:HMM:NF003976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HQP46123.1"
                     /translation="MKTTDRFLEYVKINTASDPDSTGTPSTKIQFHLAESLEKEMNAL
                     GLTEVTLSDQCVLFGSLPATPGYEKVPVIGFIAHMDTSPDFSGEGVNPQIIPDYDGKN
                     VPLGKSGKVLSVKNFPRLKKLKGCTLITTDGTTLLGADDKAGIAEIMTACEEIIEKKI
                     PHGKIRIGFTPDEEIGRGTENFDIRAFGADYAYTSDGDEEGTINFENFNACAATFEIH
                     GVNVHPGSAKNIMVNAALIACEINQMLPSGEIPSKTENYEGFYHLCDIAGDVEKATLK
                     YIVRDHSKEIFHARKESLKL"
     assembly_gap    1125..1224
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1434..2651
                     /locus_tag="PKV59_04895"
     CDS             1434..2651
                     /locus_tag="PKV59_04895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQP46124.1"
                     /translation="MATFFLILIYASFISLGLPDSVLGSAWPMMKIELHAPDDFAGYI
                     FMVVAGGTIISSLFSSRFIKRFGTGVVTFVSVLMTATALFLFHFAPSYYWFLIFAIPL
                     GLGAGAVDSGLNEFVAEHYESRHMSWLHSFWGLGAMTGPIIISKLFASGQTWRNGYLI
                     ISIIQFGLAAILFLSLPLWKKVKEREKIQQDLNPPIESDLAQTKKSSGVSVLLKHPGV
                     FPVLISIFLYCGVEQTMMLWGATYLIASRNVTPATAAAWISLFLAGITIGRFLSGFAT
                     FKLSNKMMIRLGELIILIGLTLLIFPLPSPFVQAGIFIAGLGCAPIYPCMLHETPVRF
                     GKEHAQEIMGIQMAVAYLGSTLVPPTLGAIGTRTTMIMIPFVLFAFTGIMLFSCEEFN
                     RIQVKIDSVMKSS"
     gene            complement(2716..>3771)
                     /gene="hcp"
                     /locus_tag="PKV59_04900"
                     /gene_synonym="priS"
     CDS             complement(2716..>3771)
                     /gene="hcp"
                     /locus_tag="PKV59_04900"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HQP46125.1"
                     /translation="LTLETGSYGVKGMALLDAANTETYGNPEITKVNLGVRKNPGILI
                     SGHDLRDLEMLLEQTKGTGVDVYTHSEMLPAQYYPFFKKYDHFAGNYGNAWWKQGTEF
                     ESFNGPILMTTNCIIPVKDSYKDRLFTTGATGYPGCKHIPGEIGEEKDFSEIIALAKT
                     CPAPVEIESGEIIGGFAHNQVEALADKIVDAVKSGAIKKFIVMSGCDGRMKSRSYYTE
                     FAEALPKDTVILTSGCAKYRYLKLPLGDINGIPRVLDAGQCNDSYSWAVIALKLKEIF
                     GAADINDLPIAFNIAWYEQKAVIVLLALLSLGIKNIHLGPTLPAFLSPNVAKVLVENF
                     GIAPISSVEEDVKILCA"
BASE COUNT          995 a          877 c          785 g         1014 t
ORIGIN      
        1 aaatctccgg cgcagattcc gaactcaaaa tatgctaaaa tcaagcgtaa tgaaaagcaa
       61 gacaaatgcc gttcccatta gaagagtgat cacatttcga ccgaatgttc ccgtttcgaa
      121 agcaaaaatt accgaaaaag ataacgatta ttggtggatt cggtcttttt ttccgttcgt
      181 cacgcggaag tgattctttc aacggcattc gtctccgaaa aacattgagg cataatgaaa
      241 acaacagacc gatttttaga atatgtaaaa ataaataccg catccgatcc cgattccacc
      301 ggcacaccca gcacaaaaat acaattccat cttgccgaat cgcttgaaaa agaaatgaac
      361 gctctcggtt tgaccgaagt caccctttcc gatcaatgtg ttttattcgg aagtcttccg
      421 gcaacccccg gctatgaaaa agtgccggtg atcggtttca tagcgcatat ggataccagt
      481 ccggattttt ccggggaagg ggtgaatccg cagattatcc ccgattacga cgggaaaaat
      541 gttcctttgg ggaaaagcgg caaggtctta tccgttaaaa attttcctcg tttgaaaaaa
      601 ttaaagggat gtacgttaat cacaacggac ggaaccacat tattgggtgc ggacgataag
      661 gccggcatcg ccgagattat gactgcctgc gaagaaatca tcgaaaaaaa gattcctcat
      721 gggaaaatcc gcatcggttt cacaccggat gaagaaatcg gacggggaac ggaaaatttc
      781 gacatacggg cattcggcgc cgattacgca tatacttcgg acggagatga ggaaggaacg
      841 atcaattttg aaaactttaa cgcatgtgct gcgacttttg aaattcacgg agtcaatgtc
      901 cacccgggtt ccgccaaaaa cattatggta aatgcggctt tgatcgcctg tgaaatcaac
      961 caaatgctcc cctccggaga aattccttcc aaaacagaga actacgaagg cttttatcat
     1021 ctgtgcgata tcgccggcga tgtcgagaaa gcaaccttaa aatatatcgt cagagatcac
     1081 tcaaaagaaa tttttcacgc ccggaaagaa tcattaaaac tgatnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnntggaaa tctgtacacg aatcatcatt gaaattgtta
     1261 aaattgccgc ggaaaagaaa taatctttcc ccaactctat ttacggcgat ttttaattgt
     1321 tacaaaattt atatgtaaat taaatatatt aaaaaatcaa tcaaaataat ttccggacag
     1381 aacggatcgt ttttcctgtc ttttccggaa agaacgaacg gaaatatttc ataatggcaa
     1441 cttttttcct gatcttgatt tatgcttcct tcatcagtct cggcttgcct gattcggtac
     1501 tcggatcagc ctggccgatg atgaaaatcg aactgcatgc gcccgatgat tttgccggtt
     1561 atatttttat ggtggtagcc ggcggaacga ttatttccag tttattcagc agtcgtttta
     1621 tcaagcgttt cggcaccggc gtagtcactt ttgtaagcgt tctgatgacc gcaaccgcac
     1681 tcttcctttt tcactttgcc ccttcttatt attggttttt gatctttgcg attccgctgg
     1741 ggttgggcgc cggtgcggtc gattccggct taaatgaatt tgtggcggaa cattacgaat
     1801 cacgtcatat gagttggctg cacagttttt ggggattggg cgccatgacc ggccccatca
     1861 ttatctcaaa attgttcgca tccggacaga cttggcgaaa cggttatctg attatctcga
     1921 tcatccagtt tggtcttgcc gcaatccttt tcctgtcatt gccgctttgg aaaaaagtaa
     1981 aggagcggga aaaaattcaa caggatttga atccgccgat cgaatcggat ttggcgcaaa
     2041 cgaaaaaaag cagcggagtt tccgttctgc tcaaacatcc gggagtcttc ccggtactga
     2101 tttcaatctt tttgtattgc ggagtcgaac agacaatgat gctttgggga gccacttact
     2161 tgatcgcctc acgcaatgtg acacccgcca ccgccgccgc ttggatttct ttgtttcttg
     2221 ccggaattac catcggacgg tttctctccg gttttgcgac gttcaaactg agtaacaaaa
     2281 tgatgatccg tttgggcgaa ttgattattc ttatcggctt aactttactg atttttccgc
     2341 tgccttcacc cttcgttcaa gcaggcattt ttattgcggg gttgggctgc gctccgattt
     2401 acccctgcat gcttcatgag acaccggtcc gtttcgggaa ggaacatgcg caggaaatta
     2461 tgggaatcca gatggctgtc gcttacttgg gttccacttt ggttccccct accctcggtg
     2521 cgatcggcac tcggacgacc atgatcatga tcccttttgt cctttttgct ttcaccggaa
     2581 tcatgctctt ttcatgcgag gagttcaatc gaattcaggt gaaaatcgat tctgtgatga
     2641 agtcttctta aggttccgct tagagataat caaaacagcc ggcaaaccac gccggctgtt
     2701 ttgattgtat tacaactatg cgcaaagaat ctttacatct tcttcgacac tcgaaatcgg
     2761 tgcgattccg aagttttcaa ccaatacttt cgcaacattc ggtgataaaa atgcgggaag
     2821 tgtcggaccg agatggatat tctttatccc aagagacagc aatgccagca gaacaatcac
     2881 cgccttttgc tcataccaag caatattgaa tgcgatcgga aggtcattga tatccgccgc
     2941 tccaaagatt tccttcaatt tcaatgcaat aaccgcccat gaataagaat cattgcactg
     3001 ccctgcatcc aaaacacggg ggattccgtt gatgtcaccc aagggtaact taaggtaacg
     3061 atatttcgcg caaccggaag taagaatcac ggtatctttt ggcaaagctt ccgcaaattc
     3121 cgtgtagtag gaacgagatt tcatccgtcc gtcacatccg ctcatgacga taaacttttt
     3181 aatcgcgccg gatttgacgg cgtccacaat cttatccgca agagcttcca cctgattatg
     3241 agcaaatccg ccgataattt cgccgctttc gatttcgacc ggagccggac aggtcttcgc
     3301 caaagcaata atctccgaaa aatctttttc ttccccgatt tccccaggaa tatgcttgca
     3361 gcccggatat ccggtggcac ccgtagtgaa taaccgatcc ttgtagctgt ccttcaccgg
     3421 aataatacag ttggtggtca tcaaaatcgg cccgttgaaa ctttcgaatt cggtaccctg
     3481 tttccaccaa gcattcccgt aatttccggc aaaatgatca tatttcttga aaaacggata
     3541 atattgagcg gggagcattt ccgaatgagt atacacatcg accccggtcc cctttgtctg
     3601 ttcaagcaac atttccaagt cccgcaaatc atgacccgag atcaaaatcc ccggattttt
     3661 gcggacaccc aaattcactt ttgtaatctc cggattcccg taggtctcgg tgttagccgc
     3721 atccagcaaa gccattccct tgacaccgta actgccggtc tcaagtgtta a
//
LOCUS       DAOMIV010000166         3766 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25470_length_3766_cov_4.321477, whole genome shotgun sequence.
ACCESSION   DAOMIV010000166 DAOMIV010000000
VERSION     DAOMIV010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25470_length_3766_cov_4.321477"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..235
                     /locus_tag="PKV59_04905"
     CDS             86..235
                     /locus_tag="PKV59_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HQP46126.1"
                     /translation="MKRFSPYESGQGILEYVLIIILVGVIIYVLLTLLGPAITQFIQD
                     LAAPV"
     gene            254..979
                     /locus_tag="PKV59_04910"
     CDS             254..979
                     /locus_tag="PKV59_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQP46127.1"
                     /translation="MVNTHLRQNQKTITWEILDWIFPPSCCGCGIKGAVICPSCRKSL
                     TLLEEPVCQICGKPFDPLDGCKTCRSQEMRFTSARAAFLYQGIVREAIHQLKYSGNLE
                     ISQVFANYLLSVYLKQNWEPDFVIAVPLSKEKMNERGFNQSEWIAKPLAKAIRKPYLK
                     GALRKKQDTDSQVGLGRTERIENLRGAFQAEPILVRGKNILVIDDVMTTGSTFNECAG
                     TLKASGAKDVFCLSVATTDSLKD"
     gene            1018..1584
                     /gene="raiA"
                     /locus_tag="PKV59_04915"
     CDS             1018..1584
                     /gene="raiA"
                     /locus_tag="PKV59_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQP46128.1"
                     /translation="MEKKSIIFAKNMEVSDRVEEYINKKIVKIEKFLPDVDDMKVDLA
                     YVKSARNAADRYVAQITIRGKGYILRTEERAEDIFAAFDSSLDKMLRQIDRFKGKRIR
                     GRNEKVTALTGETTGEKLEGEEEEQPLIVRRKKFNLVPMNEQEAIEQMQLLGHDEFFV
                     FYNGETASVNVLYKRHDGTYGLIDCVVG"
     gene            1873..3639
                     /locus_tag="PKV59_04920"
     CDS             1873..3639
                     /locus_tag="PKV59_04920"
                     /inference="COORDINATES: protein motif:HMM:NF018647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HQP46129.1"
                     /translation="MAEKNDQYHLITEYLKVSIPADPAGCIEITNHLFDLTISSYEIW
                     INGKKIDLSLCGKDICFPLFSEMGSTMRLPAPMPMSDQDDVKIGLNTVEIGRLTTGNR
                     IPAPEKPQSKHKWRVLRLFARDFDEDLMVDSQAIIGRRENQTLGFSFFPFDNRQTYSL
                     WFNSENAELQPAVVSMLKDIRPQSLGFRLPVDFGRMEADDTALKCFLKLCKTLEASPH
                     IRIDLNHYCGREITEVAQSAFGSLRSEDWWKPVWYFDFFVGDGFESGISAEETADKIN
                     DLSERLRSLNPDIKIILTGADPSLGSAVRWNEEMVTKCCRKIDIFGIQWLFPGVKGWL
                     RGKGPFETELATGYSAALDQALRITHGMLQNASPDQSIPIALSCWSYLRAPSQNTADY
                     RINFSKQDCFFHASQLNSIIRNSDFVSLAENGPLIGNLGLIQAEGDRIWGSAAYHLQK
                     LYLDSREIVLKVATIRDKPMQAYSWSGIPGLIEGRDIPYIDVLATRSADASNLTLLVL
                     NRHHRKRAHVRVSFRNFADLRPMEAKILRSGKFSFQNTSSKPETVYCASVPLGKYKAM
                     DHVNLDLPPYGIISMTLTASNK"
BASE COUNT         1076 a          789 c          896 g         1005 t
ORIGIN      
        1 gacggacgga ttgacaaccc ttattaaaaa agaatacaat aagaaaaagg caccataatt
       61 tattaccgga caaagggtaa acaatatgaa aagattttca ccgtatgaaa gtggacaggg
      121 aattttagaa tatgtgttaa tcattatctt ggtcggagta atcatttatg tcctgctcac
      181 acttctcgga ccggctatca ctcaattcat acaagatttg gcagcgcctg tttaaaaggg
      241 ataatatgcg ggaatggtga atacccatct tcggcagaac caaaaaacaa ttacatggga
      301 aatcctggat tggattttcc cgccgtcatg ctgcggatgc ggaatcaaag gagcagtcat
      361 ttgcccgagc tgccgaaaat ccttaacttt gctcgaagaa cctgtttgcc aaatttgcgg
      421 aaagcctttc gatccgctgg atgggtgcaa aacctgccgg tcgcaagaga tgcgttttac
      481 ttccgccaga gccgcctttt tatatcaggg tatcgtcaga gaagcgatcc atcagttaaa
      541 atattccggc aatctggaga tttcacaggt atttgcaaat tatttattat ctgtatactt
      601 aaagcaaaat tgggaaccgg attttgtaat cgccgttccg ttgtcaaagg aaaaaatgaa
      661 cgagcgcgga tttaatcagt ccgaatggat cgccaagccg ctggcaaaag cgattcggaa
      721 accgtatcta aagggagccc tccgcaaaaa acaagacacc gattctcaag tcggattggg
      781 ccgtacggag cggatcgaga acctgcgggg agcttttcag gcggaaccca tcctggtgag
      841 aggaaaaaat attttggtaa tcgatgacgt gatgacgacc ggctcaacgt tcaacgaatg
      901 cgccggaact ttaaaagcat ccggtgcgaa agacgttttt tgcttaagtg ttgcgacgac
      961 agattcatta aaagattgaa gtacgaataa ttatttattc aggaggaaca caagctcatg
     1021 gaaaagaaat ctattatttt cgcaaagaac atggaagttt ctgatcgggt tgaggaatat
     1081 atcaataaga aaattgtaaa aattgagaaa tttcttcccg atgtggatga catgaaagtc
     1141 gacctcgctt atgtcaagtc agccagaaat gccgccgatc gatatgtcgc tcagattacc
     1201 atccgcggca aagggtatat tcttcgtaca gaggaacgcg ccgaagatat ttttgccgct
     1261 tttgacagtt cactggataa aatgcttcgt cagattgatc gtttcaaagg gaagcgaatt
     1321 cgcgggcgga atgaaaaagt taccgcgctt accggagaaa ccaccggaga aaaattggaa
     1381 ggggaagaag aggagcaacc gcttattgtc cggcgtaaga agtttaatct ggtgccgatg
     1441 aacgagcagg aagccatcga acagatgcag ctcctcggac atgatgaatt ctttgtcttt
     1501 tataacggtg aaaccgcttc ggtaaatgtg ctctataaac ggcatgacgg cacttacgga
     1561 ttaattgatt gtgttgtcgg gtagtaaacc gaattgatca ccgatcatca aggcgcctcc
     1621 ggacggaagc gcctttcttt tttaaaccga ttcggttttc ggaaataaaa atcgggcaaa
     1681 ttttgtcgaa aacttcggat cgatacgaca ggttgtccaa attcaaacgc ttatataaaa
     1741 atgatccggt gcctgaagtc ttccttattg attattgcgg acattttatg atcggcgacc
     1801 gatctcaaag ccggaccaaa tctcctcgat tcgtcgtttt ttcggttaaa cttaaaaaag
     1861 gggagccgcc atatggctga aaaaaacgat caatatcatc tcattaccga atatttaaaa
     1921 gtctcgattc cggcagatcc tgccggttgt atcgagatca caaatcatct gtttgatttg
     1981 acgatttcat cttatgaaat ctggattaac ggaaagaaaa ttgatctctc gctttgcgga
     2041 aaagatattt gcttcccgct cttctccgaa atgggaagta ccatgcgcct tcccgcaccg
     2101 atgcccatgt cggatcaaga cgatgttaaa atcggtctga atacggttga aatcggacga
     2161 ttaacgacgg ggaaccgtat tccggcaccg gaaaaaccgc aatcgaaaca taagtggaga
     2221 gttttacgtc tctttgcgag agatttcgac gaagatctaa tggtcgattc acaggctata
     2281 atcggccggc gtgaaaatca aacgttggga ttctcttttt tcccttttga taatcgccaa
     2341 acctattcat tatggtttaa ttccgaaaac gcagaactgc agccggcggt cgtatccatg
     2401 ctcaaagata tcagacctca atcgttggga tttcgcttac cggtggattt cggacgtatg
     2461 gaagcggatg acacggcgtt gaaatgtttt ttgaaattat gcaaaacgct tgaagcttct
     2521 ccgcatatac gaatcgacct caaccattat tgcggcagag aaatcacgga agttgcgcaa
     2581 tcggcattcg ggtcactccg gtctgaagat tggtggaagc ccgtttggta ttttgatttc
     2641 tttgtcgggg acggctttga atccggtatt tccgcggaag agactgcaga caaaataaat
     2701 gatctttcgg aacggcttcg ctctctgaat cccgacataa agataatttt gacgggtgcg
     2761 gatccgtcat tgggatcggc cgttcgctgg aacgaagaga tggttacaaa atgttgccgg
     2821 aaaatcgata tctttgggat ccaatggttg ttcccggggg taaaaggatg gttgcgtgga
     2881 aaaggcccgt ttgaaacaga attggccacg ggatattccg ccgcgctgga tcaggcgttg
     2941 agaataacgc acggaatgct gcaaaatgct tctccggatc aatcgattcc gattgcgctg
     3001 agttgttggt cctatctcag agcaccctcg caaaataccg ccgattatcg gattaatttt
     3061 tcaaagcagg attgtttctt tcatgcttct cagctcaaca gcattatccg aaattccgat
     3121 tttgtttcgc tggcggaaaa cggtcctttg atcggaaatt tagggttgat acaggctgag
     3181 ggagatcgaa tttggggatc cgccgcatat catttgcaga agttgtattt ggatagccgg
     3241 gaaatcgtct taaaagttgc tacgatacga gacaaaccga tgcaggccta ttcttggtcc
     3301 ggtatacccg gtttgatcga aggaagagat attccttata ttgatgttct tgcgacccgt
     3361 tccgcggacg cgagcaacct gacgctgttg gttcttaacc gtcaccatcg aaaacgggcg
     3421 catgtacggg tcagtttcag gaattttgcg gatttacgac cgatggaagc aaaaatcctg
     3481 cgctcgggaa aattttcgtt tcagaatacc tcctccaaac cggagactgt ctattgtgct
     3541 tccgttccgc tggggaaata taaagcaatg gaccacgtca atttggattt acccccctac
     3601 ggaattattt ccatgacatt aaccgcttcg aataaataag tttataaccc cgacaaccct
     3661 cttcgaataa tcgagcggat ttttcggtta ttcggcgcct ttgatggttc tatattttca
     3721 atggatccgg cgaatatctg gacaactatt gaaaaaatgg tataaa
//
LOCUS       DAOMIV010000167         3764 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25486_length_3764_cov_5.052575, whole genome shotgun sequence.
ACCESSION   DAOMIV010000167 DAOMIV010000000
VERSION     DAOMIV010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25486_length_3764_cov_5.052575"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..138
                     /locus_tag="PKV59_04925"
     tRNA            64..138
                     /locus_tag="PKV59_04925"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:98..100,aa:Val,seq:cac)
     gene            complement(202..552)
                     /locus_tag="PKV59_04930"
     CDS             complement(202..552)
                     /locus_tag="PKV59_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HQP46130.1"
                     /translation="MSIPASETVILITRNGMGEGTPELQLKLISVYLKLIDENNILPA
                     AICLYTDGVKLATNGSPVIDTLKSLEKKGVRLILCSTCLNFYNLVDQVAVGIVGGMSD
                     IIDAQFKAEKVISI"
     gene            complement(754..1647)
                     /locus_tag="PKV59_04935"
     CDS             complement(754..1647)
                     /locus_tag="PKV59_04935"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQP46131.1"
                     /translation="MPENNSPIFCNYDESDYQNRFWGEGKRAYEDASEAIALERLLPK
                     SGRLMLEIGAGAGRNTLRYRGYERIVLMDYALTQLQQAQQRLGKSDRFIYVTADVYHM
                     PFADGLFDGATMIRVLHHLSEINSALAEIQRVMQYQSVFILEFANKRNLKAIFRYLTG
                     KQDWNPFSPEQVEFAELNFDNHPDTVRKVLTENGFIPEKVLTVSNLRIESLKKEKNLG
                     WMLKLESALQYLASPFKLSPSIFIRNRQSLEKPDAVEWEFFKCPNCHSYEIVEESEKV
                     HCHSCGRDYPIIDGIYDFRLK"
     gene            complement(1678..1777)
                     /gene="ffs"
                     /locus_tag="PKV59_04940"
     ncRNA           complement(1678..1777)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKV59_04940"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1864..2487)
                     /locus_tag="PKV59_04945"
     CDS             complement(1864..2487)
                     /locus_tag="PKV59_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HQP46132.1"
                     /translation="MASKSNDFYFNAFVEQSEFSIQIVEKVKNIFENYNTINLRTSMD
                     EVHLLEHTADIKHHEIMEKLSKEFIPPIEREDIVVLTQLLDDLTDYLEDIPILLYTYH
                     VKTIDQAMMQFMDVIDQSVHQVMKLLSVFSDFKKDLTIHKYIIDINRLEENGDILFHE
                     FMYKVFEEEHDARYLIGQKEMFTKFEGILDTAEDIANEVGNIIMKNS"
     gene            complement(2509..3561)
                     /locus_tag="PKV59_04950"
     CDS             complement(2509..3561)
                     /locus_tag="PKV59_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HQP46133.1"
                     /translation="MSISFSEFIYLISHNIPLLITVILTLSVIIVNGWTDAPNAIATC
                     IGTRSLKPQYALIMAAVFNFLGVFVMTILNSTVAQTVYKMVDFGTNSSEATLALCAGM
                     VAIVLWATVASFMGIPTSESHALIAGLSGAAIGLHGSFAGINGSEWIKVIYGLVLSTV
                     MGFGFGFLAYKLVKLIFQSIDRRRTQKFFRHAQVFASGAMAFMHGAQDGQKFIAVFML
                     GIALSQGETGQSVFVVPLWLMILCSLVMSLGTSIGGYSIIKSVGMNMVKLEPFQGFAS
                     DIAAASCLLISSLFSLPVSTTHTKTTAIMGVGAGKRISSVNWGIVKDMVWAWVLTFPG
                     CGLIGFLVVKLFIYLF"
BASE COUNT         1137 a          862 c          725 g         1040 t
ORIGIN      
        1 aagaaatcgg cggaactttc ttgtcagttc cgaaaaggac tgctataatg ctgtttgcac
       61 ctggggcgca tagctcagtt ggttagagcg cttgtatcac acacaagagg tccgggggtt
      121 cgagtccctt tgcgcccact tttttcttcc tttttgtcct acaatcgatc catttgattc
      181 accacagcag tgaaagttgt tttatatgga aattactttt tcggctttga attgggcgtc
      241 gataatatcg ctcatacctc cgacaatccc aaccgcgacc tgatcgacca agttgtaaaa
      301 gttcaggcag gtggaacaca gaattaagcg cacgcccttc ttttcgaggg attttaaagt
      361 atcgatgacc ggtgatccgt ttgtcgccaa ctttacgccg tcagtataaa gacaaatcgc
      421 tgccggtaga atattatttt catcgatcaa cttaagataa accgaaatca gttttaattg
      481 cagttcggga gtcccttcac ccatcccgtt ccgggtaatt aaaataaccg tttccgacgc
      541 aggaattgac atatgattct ccttatagat ccgattgccg gttgagattt tttataaaga
      601 accgaccgaa tttcaaacgg caatcatcca aataattgaa aatacttggg aaatattata
      661 cccgactaat actcattcat aaaatatttt tattttaaat ttaagaaaga agtcttatcg
      721 atttttttga gtggatagaa caatgatcga aattcatttc aatcggaaat cataaatgcc
      781 gtcgataatc ggataatccc gaccgcaaga atggcaatgc actttttcgg actcttcgac
      841 aatttcgtag ctatggcaat tcggacattt aaaaaattcc cattcgactg cgtccggttt
      901 ctccaacgac tgtcgattgc gaatgaaaat actcggactc agcttgaacg gggaagctaa
      961 atattgcagg gcggattcca gcttcaacat ccagcccaga tttttttctt tcttcagcga
     1021 ttcgattcgt aaatttgaaa cagtcaatac cttttccggt ataaacccgt tttctgtcag
     1081 tacttttctt acggtatcgg ggtgattatc aaagttcagt tcggcgaact ccacttgttc
     1141 gggagaaaaa ggattccaat cctgtttacc ggttaaatat cgaaaaatcg ctttcaaatt
     1201 tcttttattg gcaaattcca aaataaaaac ggactgatat tgcataaccc gttggatctc
     1261 agccaaagcc gaattaattt cactcagatg atgcagcacg cgaatcatcg tagccccgtc
     1321 gaacagcccg tccgcaaacg gcatatggta aacatcggca gtaacataga taaaccggtc
     1381 ggactttccc aatcgctgtt gagcttgctg cagctgagtg agggcataat ccatcagaac
     1441 aatccgttcg tatccccgat aacgtaacgt attccgcccg gctccggcac cgatctccaa
     1501 catcaatcgg ccggatttcg gcagcaagcg ttccaaagca atggcttcgg aagcgtcttc
     1561 atatgctcgc ttcccttcgc cccaaaatcg gttctggtaa tcggattcgt cgtagttgca
     1621 aaaaattgga gaattatttt cgggcataaa tccttcatca atcgattaaa aagtgcgtcc
     1681 ggacaggttt gccggcggcg cccgaatgat ccatcttatc gctgcttcct ctcggacctg
     1741 acgaggttcg atggtatccg ccgcgccggt cccgcccgag cgccttttaa gaatacccta
     1801 agcgattatt catgtcaata taaagatcac aaaagaaagt tacatccgat atccagtcat
     1861 ccgtcaggaa tttttcataa tgatattccc gacttcattg gcaatatctt cggccgtatc
     1921 caaaatccct tcaaatttcg taaacatttc tttctgaccg atcagataac gagcgtcatg
     1981 ttcctcttcg aataccttat acataaactc atggaaaagg atatcgccat tctcttcgag
     2041 ccggttgata tcgataatat atttatgaat cgtaagatct tttttgaaat cgctgaaaac
     2101 actcaaaagt ttcatcacct gatgaaccga ttgatcgatg acatccataa attgcatcat
     2161 tgcctgatcg atcgtcttaa catgataggt gtaaagcaaa attggaatat cttcgagata
     2221 atcggtcaag tcatctaaaa gttgtgtgag tacaacaata tcttctcttt caatcggagg
     2281 aataaattcc ttggacagtt tttccattat ttcatgatgc ttgatatccg ccgtatgttc
     2341 cagtaagtga acttcatcca tagaagtacg caaattgatt gtgttatagt tttcgaaaat
     2401 atttttgacc ttctcaacaa tttggatgga aaattcagat tgttcaacga aagcattaaa
     2461 gtagaaatca ttactttttg aagccatgat atccattcct ttcccacatc agaataaata
     2521 gataaacagc tttactacca gaaagccgat caacccacag cccggaaatg ttaaaaccca
     2581 agcccagacc atatccttaa caattcccca attcacagac gaaatccttt tccccgcacc
     2641 tactcccata atcgctgtgg ttttcgtgtg tgtggtactg acgggaaggc tgaaaagaga
     2701 agagatcagc aaacaactgg ctgcggcaat atccgaggca aagccttgga atggttcgag
     2761 ttttaccata ttcatcccga ccgatttgat aatgctgtac ccaccaatcg aggtgcccaa
     2821 agacataacc aatgaacata aaatcatcaa ccacaaagga acaacaaaaa cggattgccc
     2881 cgtttctcct tgagacaatg cgatgcccag cataaagaca gcgataaatt tttgtccgtc
     2941 ttgagctccg tgcataaaag ccattgcgcc gcttgcaaaa acctgggcat ggcggaaaaa
     3001 tttttgcgtc ctccggcgat caatgctttg aaaaatcaat ttcaccaatt tataggctaa
     3061 gaaaccgaaa ccgaaaccca taaccgttga caaaaccaat ccgtaaatga ccttgatcca
     3121 ttcacttccg ttgattccgg caaagctgcc atgcagaccg atagcggctc ccgaaagacc
     3181 ggcgatcaat gcatgacttt cgctggttgg aattcccata aaagatgcga cggtcgccca
     3241 aaggacaatc gcaaccattc cggcacacag cgccagagtg gcttcgcttg aattggtccc
     3301 gaaatcaacc attttataaa cagtttgagc aacggtagaa ttcaaaatag tcatcacaaa
     3361 gacgcctaaa aaattaaaga ccgcagccat aatcagggca tattgaggtt tcagagagcg
     3421 ggtaccgata caagttgcga tagcattcgg tgcatccgtc caaccgttca caatgatcac
     3481 actaagtgtc aaaataacgg ttatcagaag gggaatatta tgagaaataa ggtagataaa
     3541 ctcggaaaaa gatatggaca ttacaatacc gatttttgca tttgattatt tgatatgaaa
     3601 cgctgtttat tttaatgcaa taataacacg taaagcgaac ttgctgacgg ttttctacac
     3661 tattctgttt ttcaggcgga aatgggacaa aaaatgaaac ggtactattc tataaattcg
     3721 ttcgctatcg ggctaataaa agccgatatc atccaaattt gaca
//
LOCUS       DAOMIV010000168         3762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25510_length_3762_cov_4.743458, whole genome shotgun sequence.
ACCESSION   DAOMIV010000168 DAOMIV010000000
VERSION     DAOMIV010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25510_length_3762_cov_4.743458"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="PKV59_04955"
     CDS             <1..579
                     /locus_tag="PKV59_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46134.1"
                     /translation="AGLLPWQLFENSLRNASKSLVANRNMITKIYFPRIVLPLSSVLA
                     CIVDFFVALPILIGFMIYYQYSPNIYALLIPYYLLLTVITAFGVSLWFSAMDVMYRDI
                     GYIVPFISQLWMYLTPIAYGSGIISNPKLQFIYNLNPMVGVVNGFRSSILGEYIKLPI
                     QSMILSAVISILILVSGLFYFRRMEKQFADVI"
     gene            591..1859
                     /locus_tag="PKV59_04960"
     CDS             591..1859
                     /locus_tag="PKV59_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46135.1"
                     /translation="MNDYAVEVHDIGKRYRIGAAPAAYRTFRETLTEPFNKSKSKHHG
                     KMTDDNSFWALKDISFNVKQGQALGIIGRNGAGKSTLLKILSRVTEPTVGSAVIKGRV
                     GSLLEVGTGFHPELTGRENIYLNGAILGMRRTEIDRKLDEIIAFSEVEEFIDTPVKRY
                     SSGMYLRLAFAVAAHLEPEVLVVDEVLAVGDAEFQRKCLGKMTDVTNEGRTILFVSHN
                     MSAILRLTNETIVLEKGKMVMRAPSVEAVDYYLSHGFSQGGERFWTAEEISKDAAPFT
                     PLAIRILTDRNHVSETVRSTEPFQVEFEYRLDAPITGLRVGIYLISTRGEFIFTSFDV
                     DDPEKFTQYGTREKGKYRSRCTIPADLLNEGKYVIGVNASTFKIKRYFHEEQALTFNV
                     DGTGAPGTQWPETRSGMIRPRLNWEIEGID"
     gene            1859..3490
                     /locus_tag="PKV59_04965"
     CDS             1859..3490
                     /locus_tag="PKV59_04965"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46136.1"
                     /translation="MQSNYKQTISKILGEIPLTAEFYFQLKKQSEPGTRYSLKNIQAR
                     LPQMLADIDQFKTPNAHPEKILVFTSLHYWIEEAATIALYFAAQGHHVTFGYLPYSDW
                     QNDVNKFDLRRQDLYTKKVFEQAAEYMDIVSFLPIHPSYKPIPDELKQIIEEVSRYDA
                     QYTLQVEDVDKEHPLYKMRLERNESFARMLYPWLSANKPDHMIIPNGTIQEMGIAYRM
                     AKWLKIPVVTYEFGEQRKRIWIAQNREVMRQETDDLWEVRKNIPVETEELKKVKAMFS
                     ARQHADIWENFTRRWQGSDRVGTEKIKQELKLDDRPIVFMATNVLGDSLTLGRQTFSR
                     NMAEWIERTVQYFVERTDSQLVIRVHPGEQLTHGTSMVDVVHDLLPRLPEHIHLITPT
                     DKTNSYDLVDVATVGLVYTTTMGLEMAMNGVPVIVAGQTHYRGRGFTYDPDNWVNYYK
                     ILGSILDDPQKSILTEEQVKLAWTYAYSFFFDYPRPFPWHLVKMWDDYDKRPFSYVLS
                     KEGQAEYKSSFDYLIGKPMDWKKILAEKKNETDVE"
     gene            3487..>3762
                     /locus_tag="PKV59_04970"
     CDS             3487..>3762
                     /locus_tag="PKV59_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46137.1"
                     /translation="MSGSAPKNEVRHFYDEIGWKLESDGFYQNARYEDLRPVSADYIH
                     RCHLRLVPYFQNGGKYFLDAGSGPIQYREYLAYSEKFEFRVCLDISIT"
BASE COUNT         1040 a          742 c          945 g         1035 t
ORIGIN      
        1 gcaggccttc tcccctggca attatttgaa aattctttac ggaatgccag taaatctttg
       61 gtagcaaatc ggaatatgat cacaaaaata tattttccgc gaattgtcct tccgctgtca
      121 agcgtgctgg cttgtattgt ggactttttc gtcgcattgc ctattttgat cggatttatg
      181 atttattatc agtattctcc gaatatctat gcattactga ttccgtatta tttgcttctg
      241 accgtaataa ccgcattcgg tgtcagctta tggttttcgg cgatggatgt gatgtatcgc
      301 gatatcgggt acattgtccc gtttatcagc cagttgtgga tgtacttaac gccgatcgct
      361 tacggatcgg gaatcatttc gaaccccaag ctgcaattta tttataattt gaacccgatg
      421 gtcggtgtgg tcaacggatt tcgttcttcc attttgggtg aatatataaa attgccgatt
      481 caaagcatga tattaagtgc cgttatttcc attttgattc tggtcagcgg gctgttctat
      541 ttccgaagaa tggaaaaaca gtttgcggat gttatttaag cggagttgcc gtgaatgatt
      601 atgccgtaga agttcatgat attggtaaga ggtaccggat cggagcggct cctgccgctt
      661 atcggacttt ccgggaaact cttacggaac cattcaacaa atccaaatcg aaacatcacg
      721 ggaaaatgac ggacgataat tctttctggg cattaaaaga tatttctttt aatgtgaaac
      781 agggacaagc actcggtatc atcggtcgga acggtgcggg aaaaagtact ttattaaaaa
      841 tcctttcgcg ggtgacggag ccgacggtcg gctcggcggt tatcaaaggt cgcgtcggat
      901 ccctgctgga agtcggaact ggttttcatc cggaattaac cggaagggaa aacatttatc
      961 tgaacggcgc cattttgggg atgaggcgta ccgaaatcga tcgaaaattg gatgaaatta
     1021 tcgctttttc agaagtggaa gaattcatcg acacaccggt caaacgctat tcgagcggaa
     1081 tgtatctgag actggctttt gccgtcgccg ctcatttgga accggaagtt ttggtggttg
     1141 atgaggtttt ggctgtcggg gatgccgaat ttcaacggaa gtgcttgggt aaaatgaccg
     1201 atgttaccaa cgaaggtcgc acaatcctct tcgtgagtca taatatgtcc gcgatcctgc
     1261 ggttaacgaa cgagacgatt gtccttgaga aagggaaaat ggtcatgcga gctccctctg
     1321 tggaagcggt ggattattac ctgtctcacg gattttctca gggtggggaa cgattctgga
     1381 cagcggagga aatttcgaag gatgcggctc cgttcacccc gctggcaatc cgaattttga
     1441 ctgatcgaaa ccatgtctcg gaaacggtgc gttcgaccga accttttcaa gttgaatttg
     1501 aataccggct ggatgctccc attaccggct tgcgggtcgg aatttactta atcagtaccc
     1561 ggggagaatt tatatttacc agttttgatg tggatgatcc ggaaaaattt actcagtacg
     1621 gtacccgaga aaaagggaaa taccggagcc gttgcactat tccggcggac ttgttaaatg
     1681 aaggaaaata tgtgatcgga gtcaatgcca gtacttttaa gattaaacgc tattttcacg
     1741 aagaacaagc tctgacgttt aatgtggatg gaacgggtgc gccggggacg caatggcctg
     1801 aaacacgatc gggcatgatc cgaccgcgat taaattggga aatagaggga attgactaat
     1861 gcaatcaaac tataaacaaa cgatcagtaa aattttgggc gaaattcccc ttaccgctga
     1921 attttatttt cagttgaaaa aacagagcga acccgggaca cggtattcgc tcaaaaatat
     1981 tcaggcacgt cttccgcaga tgttggccga tattgatcaa tttaaaacgc cgaacgctca
     2041 tcctgaaaaa atacttgtat ttacgtcact tcattattgg attgaagaag cggcgaccat
     2101 cgctctttat ttcgccgcgc aagggcatca cgtcacgttc ggctatcttc cttattcgga
     2161 ttggcagaac gatgtcaata aatttgattt aagaagacag gatttatata caaaaaaggt
     2221 tttcgaacaa gcggcggaat atatggatat tgtttccttt ctgccgatcc atccttccta
     2281 taaaccgatt ccggatgaat tgaaacagat catcgaagaa gtctctcgct acgatgccca
     2341 atataccttg caggttgagg acgttgacaa agaacatcct ctttataaaa tgcgactcga
     2401 aagaaacgag agtttcgcca gaatgttata tccttggctg tcagccaata aacccgatca
     2461 tatgatcatt ccgaacggga cgattcagga aatggggatc gcttaccgaa tggcaaagtg
     2521 gttaaaaatt ccggtagtca catatgaatt cggtgagcaa cggaaacgaa tttggatagc
     2581 ccaaaatcgg gaagttatgc gacaagagac cgacgatctg tgggaagttc gcaagaatat
     2641 cccggtggaa accgaagaat taaagaaagt gaaagccatg ttttcggcac gacagcatgc
     2701 cgatatttgg gagaacttta cccgtcgttg gcaagggagt gaccgagtcg ggacggaaaa
     2761 aattaaacaa gaactgaagt tggatgaccg gccgattgtt tttatggcga caaatgtatt
     2821 gggggacagc ctgacgctcg gacgacagac attctcccgg aatatggcgg aatggattga
     2881 aaggactgtt cagtattttg tggaacgaac ggattcccag ttggtgattc gagtgcatcc
     2941 gggggaacaa ttaactcacg ggacttccat ggtggatgtc gttcatgact tattgccgcg
     3001 attgccggaa catatacact tgattacccc gaccgataaa acgaattctt atgatttggt
     3061 ggatgtggca accgtcgggc tcgtctatac cacaacgatg ggattggaaa tggcgatgaa
     3121 cggcgtaccg gtgattgttg ccggtcagac ccattatcgc ggcagaggat tcacttatga
     3181 tcccgataat tgggtgaact actataaaat cttgggttcc attctggatg atccgcagaa
     3241 atcgattttg acggaagagc aggtaaaatt ggcatggact tatgcttata gcttcttttt
     3301 tgattatccg cggcctttcc cgtggcattt ggtgaaaatg tgggatgatt atgacaaacg
     3361 gccgttttct tatgtgttga gtaaagaagg tcaagcggaa tacaaatcca gctttgatta
     3421 tctgatcgga aagccgatgg attggaaaaa gattttagct gagaaaaaga atgaaacgga
     3481 tgtggaatga gcggatccgc tcccaaaaat gaggtaagac atttttacga tgaaatcgga
     3541 tggaaactgg aatcggacgg tttctatcag aatgcccgat atgaagattt aaggccggtt
     3601 tcggcggatt atattcatcg ctgtcattta cgtctggtac cttatttcca aaacggcgga
     3661 aaatattttc tggacgcggg ttcggggccg atccaatatc gggaatattt ggcttattcg
     3721 gaaaagtttg aatttcgggt ctgcctggat atttcgatta cc
//
LOCUS       DAOMIV010000169         3760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25525_length_3760_cov_4.670175, whole genome shotgun sequence.
ACCESSION   DAOMIV010000169 DAOMIV010000000
VERSION     DAOMIV010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25525_length_3760_cov_4.670175"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKV59_04975"
     CDS             <1..390
                     /locus_tag="PKV59_04975"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HQP46138.1"
                     /translation="FFYGHPQNRFWKVISRVLNWKLAESINEKKEMLISNRIALWDVI
                     ASCEIIGADDGSIRNPKPNPIEKILLQSEVKAIFSTGKKSFHLYRKLIYPRTAVPSNY
                     LPSPSPANCKISLDELVRAYDAILPWL"
     gene            515..1186
                     /locus_tag="PKV59_04980"
     CDS             515..1186
                     /locus_tag="PKV59_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46139.1"
                     /translation="MNRTTDISQPILSVSGIRKKFGPRTVLKSISLDLKPGDCTVLQG
                     DNGVGKSTLLKILAGTLKPDEFDFIRINGEDSYNSPSYRKMIGSVNHQLMAYGDLDAL
                     DNLRFTAGLYGIKTNHQTILELLDRFDLSARVHDPVRTYSRGMQQRLSICKALLIRPI
                     LFLMDEPYTGLDQKSRTMLNEVILEQKSLSKAILITSHEPETLKSLATNFIKLEKGIL
                     IQENS"
     gene            1183..1857
                     /locus_tag="PKV59_04985"
     CDS             1183..1857
                     /locus_tag="PKV59_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQP46140.1"
                     /translation="MKSTFQTIRALIRKDMQIESHTREIFTVMLVFALSILFILNFAL
                     EQNVKVRTGSASGLIWIILILAGQLGLNQIMGIEKQGNMIEALLASPVPRSAIYLSKT
                     ISAFVFMFLMALIIHPLYCIFYNVPMISPDFILVSALGCWGYAALGVLISAMTIQTRT
                     RDILLPVLFYPLIIPLVVTTVKVTGSILSGENFSSLLSSLYQILAFDVIFTASGILGF
                     ELIVED"
     gene            1863..2561
                     /gene="ccsA"
                     /locus_tag="PKV59_04990"
     CDS             1863..2561
                     /gene="ccsA"
                     /locus_tag="PKV59_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQP46141.1"
                     /translation="MNMQIPKKLKILDWFSFFLILFTCWLIFYKTPVEMNMGLVQKIF
                     YFHVAYAWIGMVSFLVAAISAIIFLRKGLFIYDAVTESAVEIGLVFTLIAIITGSIWA
                     RPIWNTWWTWDPRLTTTSIMALIYAGYLFLRGSLDNPEKRRKITSIYSIIGCLSVPLT
                     FFSIRGVRSIHPVVFGGDTPDFNMDSQMLRTMLIAIAVFSIFYIAVMWHRIRLAELKE
                     EIEQLKLTSEEDHD"
     gene            2554..2727
                     /locus_tag="PKV59_04995"
     CDS             2554..2727
                     /locus_tag="PKV59_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46142.1"
                     /translation="MTEIPNTLNYMVAGFLVVIIGTLIYIGSLIVRWKKYSKLKVELE
                     NLLDHSKKQQKGN"
     gene            2734..>3760
                     /locus_tag="PKV59_05000"
     CDS             2734..>3760
                     /locus_tag="PKV59_05000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP46143.1"
                     /translation="MRSESSKKVLFVSGIFLIAFILRFASLGTIPLSEDEAILALQAK
                     SLASGIPDYGIAQPLYVLITAFIFKFFGPTDFTARLFPALMGIFLVSIPFFFSEKISQ
                     KKKYLFAFCLAIDPALVAWSRQADSLIPLIFLIALAIILFRRGSEKTAVALAALSFLG
                     GERFFPFLAAAGIIFALIYWINRYFLKLDFKRSEETRKQVNKQNLLIFLSVLIVAGMG
                     LLAFPEGINGIGNGIINGFKINVNHDFRSVGFVPLLIAFFVYEGSAVLLFLIKYIKSI
                     SKKEKPQVIAMSCMILFAAILSIFNQGILIIPWISLPLWFFSTDVILSFRPIKKTEID
                     LPMAIAIL"
BASE COUNT         1015 a          758 c          785 g         1202 t
ORIGIN      
        1 tttttctacg gacatccgca aaatcggttt tggaaagtaa tcagccgcgt tcttaattgg
       61 aaacttgcgg aatcaattaa cgaaaaaaaa gaaatgctta tttcaaatcg aatcgcttta
      121 tgggacgtca ttgcatcatg cgaaattatc ggcgctgatg acggcagtat cagaaatccc
      181 aagcctaacc cgatcgaaaa aatattactg cagagcgagg tgaaagccat attttctacc
      241 ggtaaaaaat cttttcatct ttatcggaaa ttaatttacc caaggacggc ggtcccgtcg
      301 aattaccttc cttcaccgag tccggcaaat tgtaaaatct cattggatga attagtacga
      361 gcatatgatg cgattcttcc atggttgtaa aaaggatctg aaacaatgga atcgaaattc
      421 tttgaattag ctgtttggat tattctggct gtcggaacaa gtcttttttt gatctggccc
      481 tttttgaaag attcgaataa ataattctca ctcaatgaat agaacgacag atatttcaca
      541 gcctatttta tccgtttccg gcattcgaaa aaaattcggt ccgaggacgg ttttgaaatc
      601 gatttcactg gacctgaaac cgggtgattg tacggttctt cagggtgata acggagtcgg
      661 taaaagcact ctgctgaaaa ttctcgccgg aacattaaaa cccgatgagt tcgattttat
      721 tcggataaac ggagaagatt cttataattc tccttcttat cgaaaaatga tcggctctgt
      781 taaccatcag ctgatggcat acggcgactt ggatgcgctt gataacctgc gatttaccgc
      841 gggtctgtac ggtatcaaaa caaatcatca aacgatcctc gagcttttgg atcgatttga
      901 cttaagcgcc agagttcacg atccggtacg gacttattca cgcgggatgc agcaacggct
      961 ttccatctgc aaagctcttt tgatccggcc gattttattt ttaatggatg aaccgtatac
     1021 cgggttggat caaaaaagtc gaaccatgct gaatgaggtc atacttgagc agaaatcctt
     1081 atccaaagcg attctgatta cctcccatga accagagaca ctgaagagcc tggcaacaaa
     1141 ttttataaaa cttgaaaaag ggatccttat acaggaaaat tcatgaaaag cactttccag
     1201 acgatccggg cattaatccg caaagacatg caaatcgaaa gtcatactcg ggaaatattt
     1261 actgtgatgc ttgttttcgc cttatcgatc cttttcatct taaattttgc gcttgaacaa
     1321 aatgtaaaag ttcgtaccgg cagtgcctct gggctcattt ggattattct gattcttgcc
     1381 ggacaactcg gattaaatca aattatggga atcgaaaaac aggggaatat gatcgaagcc
     1441 ttgttggctt caccggtccc ccgttccgct atctatctga gtaaaacgat ttccgctttt
     1501 gtctttatgt tcctcatggc attgattatt catccgcttt attgtatttt ctataatgtc
     1561 ccgatgatct ctcccgactt catcttggtt tccgctttgg gttgctgggg atatgcagcc
     1621 ttaggcgtat tgatttcggc gatgacaatt caaaccagaa caagagacat ccttttgccc
     1681 gttctgttct atcctctgat cattccattg gttgtaacga ccgttaaggt gaccggatcg
     1741 attctttccg gagagaattt ttcctctctt ttatcttctt tatatcaaat tttagcattc
     1801 gatgttattt ttacggcaag cggcattctg ggttttgaat taatagtgga ggactaaata
     1861 aaatgaacat gcaaattcca aaaaagctca aaattctcga ttggttttct ttctttttga
     1921 ttcttttcac ttgttggctt attttttata aaacgcccgt ggaaatgaac atgggactcg
     1981 ttcaaaaaat attctatttc catgtcgcat atgcttggat cggaatggtc agctttctgg
     2041 tagcggcgat cagtgcgatt atttttttgc gaaaaggcct tttcatctat gatgcggtga
     2101 ccgaatccgc cgtcgaaatc ggactggttt tcacattgat agcgatcatc acaggatcca
     2161 tctgggcgcg acccatttgg aatacctggt ggacatggga tccgcgcttg acgacaacca
     2221 gtattatggc gcttatttat gccggttatc tttttttgcg cggctcgttg gataatcccg
     2281 aaaaaaggag aaaaatcact tccatataca gcattatcgg atgtctgagc gtgccgttga
     2341 ccttcttttc gattcgcgga gtccggtcca ttcatccggt cgtattcgga ggagatacgc
     2401 ccgacttcaa tatggattca caaatgcttc gaacgatgtt gattgcgata gccgtttttt
     2461 caatttttta tattgcggtc atgtggcatc gaatccgctt ggcggaactg aaagaagaaa
     2521 tcgagcaatt aaaattaacc tcggaggaag atcatgactg aaattcccaa tacactaaac
     2581 tatatggttg ccggatttct tgttgttatt atcggaacat tgatatatat cggttcatta
     2641 atcgtaaggt ggaaaaaata tagtaagctt aaggttgaac ttgaaaatct gttagatcat
     2701 tcaaagaaac aacaaaaagg aaattaatcg gctatgcgaa gcgaaagcag caaaaaagtt
     2761 ctttttgtaa gcggaatttt tctgatcgca tttattttac gttttgcttc cctcggaaca
     2821 attcctttgt ccgaggatga agcgattttg gcattacaag ctaagtcact ggcttcggga
     2881 attccagatt acggcattgc acagcccctt tatgttttaa taaccgcatt tattttcaaa
     2941 ttcttcggtc cgacggactt tactgctcgt ttatttccgg cgttgatggg aatttttctt
     3001 gtttcgattc ctttcttctt ttctgaaaaa ataagccaaa agaagaaata cctgtttgct
     3061 ttctgcttag ctatcgaccc cgctttggtt gcctggtccc ggcaagcgga ttctttgatc
     3121 ccgctgatat tcctgattgc attagcaatt atattattcc gaagaggttc agaaaaaaca
     3181 gccgttgctt tggcagcgct ttcttttctc ggcggagaac gctttttccc gtttctggca
     3241 gctgcgggaa tcatttttgc gttgatttat tggataaata ggtatttttt gaaattggat
     3301 ttcaagcgat cggaagagac aaggaaacaa gttaacaaac agaatttatt gatttttctg
     3361 tcggttctca tcgttgcggg gatggggctc ttagcttttc cggaagggat caacggcatt
     3421 gggaacggaa taatcaacgg cttcaaaatc aatgtcaatc acgattttcg atcggtcggt
     3481 tttgttccgt tgctgatcgc cttttttgtt tatgaaggct ccgcggtcct gctttttctt
     3541 attaaataca tcaagagtat ttcgaaaaaa gagaagccac aggttatcgc aatgtcatgc
     3601 atgattttat tcgctgcgat cctttcgatt tttaatcaag ggattctgat tattccttgg
     3661 atcagccttc ctctatggtt tttttcaacg gatgtcattt tatcgttcag acccattaag
     3721 aagactgaaa tcgatctgcc gatggcgatt gcaattttaa
//
LOCUS       DAOMIV010000170         3719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25911_length_3719_cov_3.179039, whole genome shotgun sequence.
ACCESSION   DAOMIV010000170 DAOMIV010000000
VERSION     DAOMIV010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25911_length_3719_cov_3.179039"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKV59_05005"
     CDS             complement(<1..242)
                     /locus_tag="PKV59_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQP46144.1"
                     /translation="MKTHEYADYVKAAELQPNNACVIVNIGAIKFTLKEYALALEDFN
                     TAIRLQPDLVLAHYNRATVFNNMGQYKEAIAECSET"
     gene            complement(460..3141)
                     /locus_tag="PKV59_05010"
     CDS             complement(460..3141)
                     /locus_tag="PKV59_05010"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HQP46145.1"
                     /translation="MDYYLIGTDEVFKKVSSSPQGLTQAEAEKRLSEYGRNVLVQPPK
                     PTIWQRLLEQVSDPMVIMLIVAAVISGITSTIRGESIADVFIIMAVVVINSVLGVYQE
                     NKAEEAIEALQKMTAATSKVLRDGRKVVIPSSELVPGDVIILEAGDAVPADGRIISSA
                     SLKIEESSLTGESEPVSKFIDILHLENSDTVPLGDRKNMCYMGSTVVYGHGEAVVTAT
                     GMNTEMGKIAGALGEAENELTPLQRKLNDLSGILTKLVIGICVFIFIFTLIRNGNFSG
                     PVILDTFMIAVSLAVAAIPEGLVAVVTIVLSVGVTNMSRRNAVIRKLTAVETLGSAQV
                     ICSDKTGTLTQNKMTVVDTFGDDMQLLSIAMALCNDAELETSPEDLNNPEKIEVSGEP
                     TEAALVIYAAKNGLDKNKLKKEYPRIGEAPFDSKRKMMSTVHRHDGKIIQFTKGAPDE
                     VLTKCTKWLHNGSVIPLTDELRNEIMEANNAMAANALRVLAGAMKEYPAEPKSYEAEE
                     IENDLTFLGLMGMIDPIRPEVIGAIKECSEAGITPIMITGDHKATAIAIAKQLGILES
                     EDQAITGAQLDHISDEEFEKMIENIKVYARVQPEHKTRIVKMWKKKGMVTAMTGDGVN
                     DAPSIKSADIGVGMGITGTDVTKNVADMVLADDNFATIVSAVEEGRRVYDNILKVIQF
                     LLSSNLAEVIAIFVATLIGFQILRPVHILWINLITDSIPALALGVEKAEGDLMKRKPR
                     DPNSHIFADGMGFDIGYQGVMIAIVTLVSYFVGHYLEAGRWEITQSPDGVTMAFLTLS
                     MAEIFHSINMRSRRNSIFSMHSINKFLILAMVIALILTTGVIEIPSIAALFEFETISL
                     NEYMTAMALAISVIPIVEIVKYFQRKRNSNYNGTVNK"
     gene            complement(3400..>3719)
                     /locus_tag="PKV59_05015"
     CDS             complement(3400..>3719)
                     /locus_tag="PKV59_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQP46146.1"
                     /translation="VLPAEKGRDSVTEYFTKESFPNHTLIEAHPLTGRTHQIRVHMAF
                     LKCPIVGDVLYGYRKKSVPIHRHFLHAARLTILLPGKESPMTFEAPLPPELEDVLNDL
                     RSA"
BASE COUNT          982 a          907 c          756 g         1074 t
ORIGIN      
        1 atcgtttcac tgcattcggc aatcgcttct ttgtattgcc ccatattgtt gaaaacggtt
       61 gcgcggttgt aatgagcgag aaccaggtcg ggctgcagac gaatggctgt attaaaatct
      121 tcgagagcta acgcatattc tttgagagta aacttaatag caccaatatt tacaattaca
      181 catgcattat ttggctgtaa ttcggcagct tttacataat cggcatattc atgagttttc
      241 atggattcat ctccttttcc ccttctaatt atacagggaa tccctcttcg aaccgcagat
      301 atccacggac cttgcaaacc tttcggagtc ttccaaaaat cggaataatt ctgcgaaaac
      361 cgagaatcct ttgggttcga aaagctttct atctctgaaa agaaaagggt acaaacggtt
      421 tttccgctgt accctttttt tatttttttg ataatcgaat tacttgttca cggtaccgtt
      481 ataatttgaa tttcgtttgc gttggaaata tttgacgatc tcaacaatcg ggataaccga
      541 gattgccaac gccatggccg tcatatattc gttgagcgat atcgtctcaa actcgaataa
      601 tgcagcaatt gaagggattt cgatcacacc ggttgtcaga atcagagcga ttaccatcgc
      661 taaaatcagg aatttgttga ttgaatgcat cgaaaaaata gaattccgac gggagcgcat
      721 gttgatcgaa tggaagatct ccgccatgga taaggttaaa aatgccattg ttaccccatc
      781 cgggctttgg gtgatttccc atcttccggc ttccagataa tgaccgacga aataactgac
      841 gagggtcaca atcgcaatca taacgccctg atacccgata tcgaatccca tcccgtccgc
      901 aaagatatgc gagtttggat ctctcggttt ccttttcatc agatctcctt cagctttttc
      961 aacccccagc gctaaggcgg gaatcgaatc cgtaatcagg ttgatccaaa gaatatgaac
     1021 cggcctcaaa atttgaaaac cgatcagcgt agccacgaaa attgcgatga cttccgcaag
     1081 gttagaagaa agcaagaatt ggatcacttt cagaatattg tcataaaccc ggcgaccttc
     1141 ctcgacagcc gatacgatgg ttgcaaagtt atcgtctgcc aaaaccatat ccgcgacatt
     1201 cttggtcaca tccgttccgg taatccccat tccgacaccg atgtccgcac ttttaatgga
     1261 aggcgcatcg ttaacaccgt caccggtcat cgcggtgacc atcccctttt ttttccacat
     1321 ttttacgatt cgggtcttat gttccggttg cactcgggca tacaccttga tattttcgat
     1381 cattttctcg aattcttcat cgctgatatg atccaactga gctccggtaa ttgcctgatc
     1441 ttcgctctca agaatgccca attgtttcgc gatcgcgatc gcggtcgctt tatggtcacc
     1501 cgtgatcatg atcggcgtaa ttccggcttc gctgcattcc ttgatcgccc cgatcacttc
     1561 cggtcgaatc gggtcaatca tccccattaa tcccaggaag gtcaaatcgt tttcgatttc
     1621 ttccgcttcg tatgacttcg gttcggcggg atattccttc atcgcaccgg caagaacccg
     1681 caaagcattt gccgccatcg cattattggc ttccatgatc tcatttcgta actcatcggt
     1741 taacggaatc acagatccgt tatgaagcca tttggtacat ttggtgagca cttcatcggg
     1801 tgcacctttg gtaaattgaa taattttgcc gtcatgccgg tgaaccgttg acatcatttt
     1861 tcgtttggaa tcaaaaggag cttcaccgat tcgagggtat tcttttttaa gcttattttt
     1921 gtcaaggccg ttctttgccg catagattac caaggcggct tcggtcggct ctccggagac
     1981 ttcgatcttc tccggattgt tcaaatcctc aggagaagtc tccaattcgg catcgttgca
     2041 taaagccatg gcgatcgaga gcaactgcat atcatcgcca aatgtatcaa ctaccgtcat
     2101 tttattttgg gtcaaagtgc ccgttttatc cgaacagatc acttgcgcgg accctaatgt
     2161 ttccacggcg gttaatttcc gaatgaccgc atttcgacga gacatgttgg tcacaccgac
     2221 cgaaagaaca atcgtgacaa ccgccaccag tccttcggga atcgcagcga cagccaaact
     2281 gaccgcgatc ataaacgtgt ccaaaataac cggacccgag aaatttccat tgcgaattaa
     2341 ggtgaaaata aagataaaaa cacaaatccc gattaccagt ttggtaagga ttccgctcaa
     2401 gtcgtttaat tttctctgaa gaggggtcag ttcattttcg gcttcaccca aagcaccggc
     2461 gatttttccc atctcggtgt tcattccggt agccgttacc accgcttccc catgtccgta
     2521 aacgaccgtg cttcccatat aacacatatt ttttcgatca cccagcggaa ccgtatcgga
     2581 attttccaaa tgcaggatat cgataaactt actcaccggt tctgattcac cggttaacga
     2641 ggattcttcg attttcaaac tggcgctgct gataatccgg ccgtcagccg ggacggcatc
     2701 gccggcttcc aaaatgatca catcccccgg aactaactcc gagctcggaa tgaccacttt
     2761 tctcccgtcc cgtaaaacct tggaagtggc tgccgtcatc ttctgtaacg cttcaatcgc
     2821 ttcttccgct ttattttcct gataaacgcc caaaaccgaa ttgatgacta ctacagccat
     2881 aataataaat acatctgcga tggattctcc ccgaattgtc gaagtaatcc cggaaatgac
     2941 tgcggcaaca atcaacataa taaccatcgg gtcggaaact tgttctaaca agcgttgcca
     3001 aatagtcggt ttcggcggtt gaacaaggac attcctaccg tattccgaca gtcttttttc
     3061 agcttcagct tgtgtcaacc cttgtggcga actggaaact tttttaaaaa cttcatcggt
     3121 gccaattaag taataatcca taaaatttgt ccttttcaga atattatgtt tgattctata
     3181 aaaaaacaag actcatgaaa ctttaataga agtttcatga gtcttgttat ttctgattga
     3241 ccgggttttt ggccgtgctg acggtttcct cacgtacgaa tggcgcacgt taactactcc
     3301 ctcatggata attcttctct attatattac ataatcttta aaacagtcag agtgttttca
     3361 taataaataa ttatcaattc tatttatgaa tcgaatgaat caggccgatc gtaaatcgtt
     3421 cagaacatct tccagttcgg gaggtagggg cgcttcaaaa gtcatcggag attctttacc
     3481 gggcaacaga atcgtcaaac gtgccgcatg aagaaagtgt ctatgaatcg gcacggattt
     3541 ttttcggtat ccatacaaca catctccgac aatcgggcat ttcagaaaag ccatatgaac
     3601 ccgaatttgg tgggtgcgtc ccgttaaagg atgagcctca atcagggtat ggttgggaaa
     3661 tgattctttg gtaaagtatt cggttacgga atcccgacct ttctcggcgg gtaatacag
//
LOCUS       DAOMIV010000171         3718 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_25923_length_3718_cov_3.784876, whole genome shotgun sequence.
ACCESSION   DAOMIV010000171 DAOMIV010000000
VERSION     DAOMIV010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25923_length_3718_cov_3.784876"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKV59_05020"
     CDS             <1..391
                     /locus_tag="PKV59_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HQP46147.1"
                     /translation="ATNRDHILASENGWLPGGGVMVSAVETCTQKKPIVIGKPQPYMI
                     EIAMEKFGLDKSEVVAVGDRYDTDIIGGINAGCHTVMVLSGVDTKESVSSFQKQPTLI
                     CKDLEEFAEILIRQKNNLDPINRTDKL"
     gene            388..1419
                     /gene="rlmN"
                     /locus_tag="PKV59_05025"
     CDS             388..1419
                     /gene="rlmN"
                     /locus_tag="PKV59_05025"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQP46148.1"
                     /translation="MKTCFFDLNYEELLSILKEWQEPEYRAKQLWNLVYRNLNFVPQN
                     FSNIPKELREKLDETFEFQPIKIIRNVSSSNQQANKYLLGLTDGKKIEAVLMRYRERT
                     TICISTQVGCAMNCAFCATGQMGFTRNLTSGEIAGQVFFFVKELEKEDRKLTNLVLMG
                     MGEPFHNYANVMKGIDLINDPEGFNFGERRITISTVGIIPMIEKFTKAKRQINLAVSL
                     HAPTNTIRNQIIPINKKYPIEDLLAACKAYTDYTGRRITFEYAMISGLNDSIEQAVQL
                     SKLLRGMLCHVNLIALNPTRKYDRTGSSRESINNFYSILDKAGIPCSIRLNRGVDIGA
                     GCGQLLAQA"
     gene            1473..2390
                     /gene="ftsY"
                     /locus_tag="PKV59_05030"
     CDS             1473..2390
                     /gene="ftsY"
                     /locus_tag="PKV59_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQP46149.1"
                     /translation="MAEFLSKWMKGLDKTRKVTFSRLASVLGTNEIDEYLWDDLEAIL
                     IQADIGVETSNEIIESLKKTVLDQGLYKSSDLVAYLKNELIQRLPESRDPLDESEKIK
                     PYVILMVGVNGSGKTTTTAKLAKKYTDAGKKVMIAAGDTYRAAAIEQLQVWGNRLNIP
                     VIAGKEGGDSAAVAFDAVQAAIARNMDILIIDTAGRLQSRYNLMEELKKVHRVIGKAL
                     PGAPHAVWIVLDATTGQNALIQAKAFKEAVKVNGVILAKLDNSAKGGMAFAITQELDL
                     PIIYAGLGEKADDLERFNREDFVNGIIGD"
     gene            2400..3479
                     /gene="prfB"
                     /locus_tag="PKV59_05035"
     CDS             2400..3479
                     /gene="prfB"
                     /locus_tag="PKV59_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQP46150.1"
                     /translation="MQESIIKMNSIKQKLNELTAHLDLETKKKHLSDLQKESQKPDLW
                     DNQDKAQNLLKEINDLQDEISDWEGLSEKVDDTIELLEIGDTSIGEELQNDINSIEKE
                     VRQKEIQVLLSGKYDSGNAILAIHAGAGGTEAHDWAAMLQRMYLRWAETHHYTADIVD
                     FTPGEEAGTKTITMSITGKYAYGYLKSEKGTHRLVRLSPFDAAHRRHTSFAMVEVLPE
                     APDTEEINIPPEDIRIDVFKSSGAGGQNVQKNSTAVRLLHIPTGIIVTCQNERSQTQN
                     RENAMKVLKSRLLEIKHQEHEKELGELRGDYVKAEWGSQIRSYVLHPYQMVKDHRTDY
                     EVGNAQAVLDGDLDGFMEAYLNYKK"
BASE COUNT         1197 a          704 c          854 g          963 t
ORIGIN      
        1 tgcgacgaat cgggatcata ttttggcatc cgaaaacggc tggcttccgg gcggtggcgt
       61 aatggtcagc gcggtcgaaa catgtacgca aaagaagcct attgtgatcg ggaaaccgca
      121 gccctatatg attgagattg ctatggaaaa attcgggttg gataaatcgg aagtagtcgc
      181 ggtgggcgat cgatatgata cggatattat cggaggtatc aatgccggct gtcatactgt
      241 aatggttctt tccggagttg atacaaagga atcagtttca agttttcaaa aacaacccac
      301 gctgatctgt aaagatttgg aagaattcgc agaaatttta ataagacaaa aaaataatct
      361 tgacccaata aacagaacgg ataaactatg aaaacttgtt tctttgattt gaattatgaa
      421 gagcttcttt ccattctgaa agaatggcag gaaccggaat accgggcaaa acaactttgg
      481 aaccttgttt accgaaattt aaattttgtt cctcagaact tcagtaacat tcctaaggaa
      541 cttcgcgaga aactcgatga aacatttgaa tttcaaccga ttaagataat ccgtaacgta
      601 tcttcttcta atcaacaggc aaataaatat ttactcggac ttacggacgg aaagaaaata
      661 gaagcagtct tgatgcgtta tcgcgaacga acgaccatat gtatttctac gcaggtaggc
      721 tgcgcaatga attgtgcttt ttgtgcaacc ggacagatgg gctttacaag aaacctgact
      781 tcgggagaaa ttgcggggca ggtttttttc ttcgttaagg aactggaaaa agaggaccga
      841 aaattaacca atcttgtgtt aatgggaatg ggagagccct ttcataacta tgcgaatgtt
      901 atgaaaggga ttgatttgat aaatgacccg gaagggttta atttcggaga acgaagaatt
      961 accatctcaa ccgtggggat tattccgatg attgagaaat ttacgaaagc aaaaaggcag
     1021 ataaatcttg ccgtctccct ccacgcaccg acaaatacca ttcgaaatca aattattccg
     1081 atcaacaaaa aatatccgat cgaagatctt cttgcagcct gtaaggcata tacggattat
     1141 accggacgaa gaatcacttt tgagtatgcg atgatcagcg gcttaaatga ttcgatcgaa
     1201 caagcagtcc aactcagtaa attgttgagg gggatgcttt gccatgttaa tcttattgca
     1261 ttgaatccga ctcggaaata tgatcgaacc ggctcttcaa gggagagcat taataatttt
     1321 tattccattt tagacaaagc cggcattcct tgttcaatca gactcaaccg cggagtggat
     1381 atcggcgcag gatgtgggca attacttgct caagcatgat ataatctatc cgcttaatac
     1441 agggaaagaa tttttttgat tgtagaggaa taatggctga atttttatca aaatggatga
     1501 aaggtcttga taaaactcgg aaagttactt tttcaagact ggcatccgtt ttaggaacga
     1561 atgaaatcga cgaatatctg tgggatgatt tggaagccat tctcattcag gcagacatcg
     1621 gcgtcgagac ttcaaacgaa ataatagagt ccctaaaaaa gacggtttta gatcaggggt
     1681 tatataaatc atcggacttg gttgcttatc ttaagaatga actgattcaa aggcttcccg
     1741 aaagccgtga cccgttggat gaatccgaaa aaatcaaacc ttatgtgatt cttatggtcg
     1801 gtgtcaatgg atcagggaag acaaccacaa ccgccaaact cgcaaaaaaa tatacggacg
     1861 ccggcaaaaa ggttatgata gcggccggtg atacctatcg agcggcggca attgagcaac
     1921 tccaagtttg gggcaatcga ttgaatatcc ctgtcattgc aggcaaagaa ggcggcgatt
     1981 ctgctgcggt ggcttttgat gcggttcaag cagcgattgc ccggaacatg gatattctga
     2041 taatcgatac tgccggacga ttgcaatccc gttacaattt gatggaagaa ctgaagaaag
     2101 ttcaccgtgt aatcgggaaa gcgctccctg gagcgcccca tgcggtttgg atcgtattgg
     2161 atgcaacaac cgggcaaaat gcccttatcc aagcgaaagc ttttaaagaa gcggttaaag
     2221 ttaatggtgt catcctggct aaattggata attccgcaaa aggaggaatg gcatttgcaa
     2281 ttactcaaga attggatttg ccaattattt atgccggatt aggtgaaaaa gcggatgatt
     2341 tggagagatt taatcgcgaa gattttgtta acggaataat cggagattaa taagcaaaga
     2401 tgcaggaatc tattattaag atgaattcca tcaaacagaa actcaatgag cttaccgctc
     2461 atttggatct ggagaccaag aaaaaacatt tatcggactt gcagaaagaa tcccaaaaac
     2521 cggatctttg ggataatcaa gataaagctc aaaatttgtt gaaagaaata aacgacttac
     2581 aagatgaaat ctcggattgg gaaggattat ccgaaaaagt ggatgacacg attgaattat
     2641 tggaaatcgg tgatacatcc atcggagaag agcttcaaaa cgatataaac tcgattgaaa
     2701 aagaagtccg acaaaaagaa atccaagtct tactttccgg aaaatatgac agcggtaatg
     2761 ccattctcgc gattcatgcc ggtgccggcg gtaccgaagc acatgattgg gctgcgatgt
     2821 tgcaacggat gtacttgcgt tgggcagaaa cgcaccatta tacagcggat attgttgact
     2881 ttactcccgg tgaagaagcc ggtaccaaaa cgatcacaat gtcaatcacc ggtaaatatg
     2941 cttacggata tctgaaatcg gaaaaaggca ctcatagatt ggttaggctc tcgcctttcg
     3001 acgcagctca ccgacggcat acttcttttg ccatggtaga agtattaccc gaagcaccgg
     3061 ataccgaaga aataaacatc ccaccggaag atattcgcat tgacgtattt aagtcttccg
     3121 gggcgggcgg tcagaacgtt caaaagaact caactgccgt tcgattattg catatcccga
     3181 ccggaattat cgtgacctgt cagaatgaac gttcacaaac ccaaaatcgc gaaaatgcga
     3241 tgaaagtgtt gaaatccaga ctgttggaaa taaaacatca ggaacatgaa aaagaactcg
     3301 gtgaattacg aggtgattac gtcaaggcgg aatggggaag tcaaattcgg tcttatgttc
     3361 tccaccccta ccaaatggta aaagatcatc ggaccgatta tgaagtcgga aatgcccagg
     3421 ctgtcttaga cggagatttg gatggcttca tggaagctta tctcaactat aagaaataat
     3481 atgagataat acttaaacat ctcatatatc taaagcgtca aaatacattt aaaggatcaa
     3541 tcctatgaag aagacgtttt ccgaattcta tgaatttatt gccaaaggca atgtaatcgg
     3601 tctggctatc ggcgtgatca tgggcacagc gttcagcgcc atcgttaatt cattaattaa
     3661 cgatgttctg atgcctgtta tcggtttgct gacttccggg gtcgatttta aaagctgg
//
LOCUS       DAOMIV010000172         3691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_26187_length_3691_cov_4.632288, whole genome shotgun sequence.
ACCESSION   DAOMIV010000172 DAOMIV010000000
VERSION     DAOMIV010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26187_length_3691_cov_4.632288"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="PKV59_05040"
     CDS             <1..302
                     /locus_tag="PKV59_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46151.1"
                     /translation="RTVHRLSQFLRITGCREGDIIDESVICRAILHPQGGFKNAPQGC
                     CKIAVLNQADTAERIKHAENISNYLLKHGVDEVWITSLDPETDKNKRGIIKELKK"
     gene            299..1129
                     /gene="yqeB"
                     /locus_tag="PKV59_05045"
     CDS             299..1129
                     /gene="yqeB"
                     /locus_tag="PKV59_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HQP46152.1"
                     /translation="MNQKNCHPREITAFRGAGDLASGIALRLWRSGYRVILSELPCPL
                     CIRRSVAFANAVYEGHMAVEGIESILIHDIAEAETIWAEDKIPLLIDPDISQIKELNP
                     SVIIDARIMKSYRDDSHLSDAPLVIGLGPGFVAGENAHAVIETNRGHDLGRVFWKGSA
                     EPDTGTPGMIGGEGPKRVQKAPASGIFTPKVEIGDFVKEGDLLALVGDTEIRSPLTGM
                     VRGVIYPGIFVNKGLKIMDIDPRGERRYCFTASDKANALGGSVLEAILTWNVRESARQ
                     "
     gene            complement(1089..2123)
                     /locus_tag="PKV59_05050"
     CDS             complement(1089..2123)
                     /locus_tag="PKV59_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HQP46153.1"
                     /translation="MREILQAIVDAHAGREDLVMATIIDNVGSSPRSAGTKMLIKPDL
                     SIIGTIGGGKLEANAILAAKEVFQSKKSNLFHFILNGEDAAKSDMICGGSGDVLLVFL
                     PWDDPETTLVFEKALDAAVGNQEGWLITQFRENGVDTNKFCFVDRQGEKTGNLIINGE
                     FLSDMKGTPDQIAIHSDLLLNSEILVEKIKERSKLYIFGGGHVAKETARLADEVSFET
                     IVLDDRAEFANKERFPNSKIMVVPDYKSLPDLPIDHQSFVVILTRGHLGDYDVLKEML
                     KTDAFYIGMIGSIHKRNLIYERLLNEGVSQKEIDRVHSPIGLPINGETPTEIAISIIA
                     ELILERSKLK"
     gene            complement(2164..2715)
                     /locus_tag="PKV59_05055"
     CDS             complement(2164..2715)
                     /locus_tag="PKV59_05055"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQP46154.1"
                     /translation="MSRKQDPLSVEFLLLGIIRKQPIHAYDLDKMLKEKEEFSVVWRF
                     NQSQLYAILDKLEKNNLIQSEITQGSAFPFRKIYTLTQEGEDAFQAWKVRPVEKPREL
                     RSDFLVKLYFLKDEPYQIFESVIKQQIMLCNQWIELQNTHLKNQPDEAVYLRMVYQFR
                     LESLRSNVTWLNECLSIRKNEED"
     gene            2967..3155
                     /gene="rpmB"
                     /locus_tag="PKV59_05060"
     CDS             2967..3155
                     /gene="rpmB"
                     /locus_tag="PKV59_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQP46155.1"
                     /translation="MAKCEICGKTTVFGHNRSFSQRATNRAFYPNLQRVTVYENGQRV
                     QKTLCTKCIRTMAKLPKE"
     gene            complement(3224..>3691)
                     /locus_tag="PKV59_05065"
     CDS             complement(3224..>3691)
                     /locus_tag="PKV59_05065"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQP46156.1"
                     /translation="DVHLMIIQPEKHIERFIEAGADILTVHCENNPNIHSTLLNIRKM
                     GASPAIALNPGTPAEMVEPLLPFLDMVLVMTVNPGFGGQEFIPEMLEKMRKIRKWIDE
                     KQLNVRIQVDGGINHETAGQCFAAGADTFVAGTSIFRNSNGYAAGIRELKNLC"
BASE COUNT         1050 a          823 c          792 g         1026 t
ORIGIN      
        1 agcggacagt ccatcgtttg tctcaatttc tgagaataac cggatgccga gaaggggaca
       61 ttatcgatga atccgttatc tgccgtgcca ttcttcaccc gcagggggga tttaaaaacg
      121 caccgcaagg atgttgcaag atcgcggtat tgaatcaggc tgacactgcg gaaagaataa
      181 agcacgctga gaatatttca aattatttac tgaaacacgg cgtagatgaa gtctggatca
      241 caagtctcga tcccgaaacg gataaaaata aacggggaat tataaaggaa ctgaaaaaat
      301 gaatcaaaaa aactgccatc cgagagaaat caccgccttt cgcggagcgg gtgatttggc
      361 aagcggaatt gctttgcgtt tatggcgttc cggttaccgc gttattttga gtgaattgcc
      421 ttgtccgtta tgcattcgac gcagtgtggc gtttgccaat gcggtatatg aaggccatat
      481 ggcggtcgag ggcatcgaaa gcatcctgat ccacgatatc gccgaagcgg agacgatttg
      541 ggcggaagat aaaatcccgc tattgattga tccggatatt tctcaaatca aagaattaaa
      601 tccttcggtc attatcgatg cgcgaattat gaaatcttat cgggacgatt ctcatttgag
      661 tgatgctccg ttagtcatcg gattaggtcc gggtttcgtt gccggagaaa atgcacatgc
      721 ggtaatcgaa acaaatcgcg ggcatgattt aggtcgggtt ttctggaaag gcagcgccga
      781 gccggatacc ggaactcccg gaatgatcgg cggtgaaggt ccgaaacggg ttcaaaaagc
      841 tcccgccagc ggcatcttta ctccgaaagt cgaaatcggc gatttcgtca aagaagggga
      901 tttattggca ttggtcgggg ataccgaaat tcgcagcccg ctgaccggca tggttcgcgg
      961 tgtcatctat ccgggaattt tcgtgaataa aggcttgaaa ataatggata tcgatccgcg
     1021 cggagagcgc cggtattgtt tcacagcttc agacaaagcc aacgctctgg gcggatctgt
     1081 tttggaggct attttaactt ggaacgttcg agaatcagct cggcaataat gctgatcgca
     1141 atttccgtgg gggtttcacc gttgatcggc aatccgatcg gagaatgaac ccgatcaatc
     1201 tctttttgtg agacgccttc gttcaataag cgttcataaa tcaaatttcg tttatgaata
     1261 ctgccgatca ttccgatata aaatgcatcg gtcttcaaca tttccttaag cacatcataa
     1321 tcgcccaaat ggccgcgagt caaaatcacc acaaaacttt gatgatcaat cggaagatcc
     1381 ggtaagcttt tataatcggg aacgaccatt attttcgaat tcggaaaccg ttccttattg
     1441 gcgaattcgg ctcgatcgtc cagtacaatt gtctcaaaag aaacctcatc ggcaagccgt
     1501 gcggtttctt tggcgacatg accgccgcca aaaatgtaca gcttgcttcg ttctttaatt
     1561 ttttccacca gaatttccga attcaggaga agatcggaat gaatggcaat ctgatccgga
     1621 gttcctttca tatccgagag gaattccccg ttgataatca aatttccggt tttttctccc
     1681 tgcctatcta caaaacaaaa cttatttgta tcgacgccgt tttctcgaaa ttgcgttatc
     1741 agccatcctt cttggttccc gacagctgcg tccagtgcct tttcaaatac aagggttgtt
     1801 tccggatcat cccaggggag gaaaaccaac agaacatctc ccgatccgcc gcagatcata
     1861 tcggatttcg ccgcatcttc accgttcaaa atgaaatgaa aaagattgct cttcttcgac
     1921 tgaaaaacct ctttggcagc cagaattgca ttcgcttcga gttttcctcc gccaattgta
     1981 ccgataatcg aaagatcggg tttaatcaac atttttgtcc ctgccgaccg cggagaagag
     2041 ccgacattat ctatgattgt cgccatcacc aaatcttccc gtccggcatg ggcatcgaca
     2101 atcgcctgta atatttctcg cattatttat cctttaaatt gaaatatgag aaccccggtc
     2161 tgtttaatct tcttcgttct tccgaatgga cagacattca ttcaaccaag tgacgtttga
     2221 ccgcaaactc tccaatcgaa attgatagac catcctgaga taaacggctt catccggttg
     2281 gtttttcaaa tgagtgtttt gtaactcgat ccattgatta caaagcataa tttgttgttt
     2341 aatcactgat tcaaaaattt gataaggttc atctttcaaa aaatagagct tgaccaaaaa
     2401 atccgaacga agctcacgcg gtttttcaac cggtcgaact ttccaggctt gaaaagcatc
     2461 ttcgccttct tgcgttaagg tataaatttt tcggaaagga aacgcggatc cctgagtaat
     2521 ttccgattgg atcagattat ttttttccaa tttatccaaa atcgcatata actggctctg
     2581 attaaatcgc caaacgacac tgaattcttc tttttctttg agcattttat ccaaatcgta
     2641 agcatggatc ggttgcttgc gaatgatacc caacaataaa aactcaacgg aaagcggatc
     2701 ttgtttacgg gacatggctg atactcctct ttcttaacaa ttttatcaaa tatttgttca
     2761 attaataagg attttttaat atcgatctat gcaaaatcct gttttatcaa caaaatcctc
     2821 aaatttgaaa atattatggt aaaatcattt tctgctgggt cgaagggaca acagcattcg
     2881 atcgaaatcc cgatttcgta aatagtttag ggttcgtatc acaatcggga gtcctgaaga
     2941 acaaacggaa ggaaaggaag ataaaaatgg caaaatgtga aatatgtggg aaaacgactg
     3001 tattcgggca caacagaagt ttttctcaac gggcaacaaa ccgtgcattt tatcccaatt
     3061 tgcaacgtgt taccgtttat gaaaacggac agcgtgtcca gaagaccctg tgcacgaaat
     3121 gcatccgcac catggcaaaa ttaccaaaag aatgattcga aacgcaaact gatcgctcca
     3181 agcagatccg atgacttgga gcgatttttt atttaaacca gtctcaacaa agatttttta
     3241 attcgcgaat acctgcggca taaccgttgc tgttccgaaa gatggacgtt cccgcgacaa
     3301 aagtatcggc acccgcggca aagcactgcc cggcagtttc atggttgatt cctccgtcca
     3361 cttgaattcg gacattcaat tgtttttcat caatccattt acgaatcttt cgcatttttt
     3421 ccaacatttc cggtatgaat tcttgtccgc cgaaaccggg attaacagtc atcaccaaca
     3481 ccatgtcaag aaaaggcaac agaggttcaa ccatttccgc aggtgttccg gggtttagcg
     3541 caattgccgg gctggcaccc attttacgaa tatttaagag agtggaatga atattcggat
     3601 tattttcgca atggacggtc aaaatatccg ccccggcttc aataaatcgt tcgatatgtt
     3661 tttcaggctg gataatcatc agatggacat c
//
LOCUS       DAOMIV010000173         3668 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_26415_length_3668_cov_3.849433, whole genome shotgun sequence.
ACCESSION   DAOMIV010000173 DAOMIV010000000
VERSION     DAOMIV010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26415_length_3668_cov_3.849433"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKV59_05070"
     CDS             <1..989
                     /locus_tag="PKV59_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46157.1"
                     /translation="VTDPVLAQMLQTFPPENLNSVVLKLPAVKTVTFTATATETETVT
                     PTVTLTPMPPTGTFTSIPTLTVTNTFTAVPTATVRPASPTPWPSPTPYQIIIVVVTAT
                     PKPTMPVWITSTPIRWVTATPIYWYTATPYWIYRTATPIPYVPPVWNYPTAYTYPTKS
                     GGNTYPIWTPYTPKASSTPKPTVNPASLFEFTYVTEKMSADNIPVKWNYTGMKEYMIN
                     YLTADRTLYPGFAMEWTEASKVCNASACTFEIKSIPDQLLKQGSFYIQLQAKDSNGRI
                     YQSDPIGLQVAGQTTPQVTGTPKSEEPEKKPNFIVRFFRWLFGPIIRLFGGK"
     gene            993..1796
                     /locus_tag="PKV59_05075"
     CDS             993..1796
                     /locus_tag="PKV59_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HQP46158.1"
                     /translation="MDFFVYLSKFLPTLVYPAGILFISLILALAFSKHPKWQKRMVIF
                     ALIVLFVCGNPFPTALLTRNLENSFLPFDGAQKAKVIVVLGGGTESKEYPRQTVEISG
                     AGDRILYAADLWKEGYAEYLLFGGAYYELLSGEKRSVASDMAEVAESLGIPKDKIILQ
                     EKSLNTYEEAVEDAAVLKKMNVDEVILVTSATHMRRAAGCFEAQGLKVIPAATDYGYT
                     DKSWENLMTLNWEKLYTYIIPQSSNMSSLETALKEYIGIFVYRLRGWME"
     gene            2050..>3668
                     /locus_tag="PKV59_05080"
     CDS             2050..>3668
                     /locus_tag="PKV59_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46159.1"
                     /translation="MKQFRTALIVMITCIVFFSTASLVAADETGYLRVAYPSTSLSTI
                     DQALVSDNSQYVESMNVGVLRQNEFSGALEFGMATDQQMSEDGTVFTYKLLENVPWVR
                     YNPATDQVEKVLDCDGNVRMVKAQDFVYAVERVLRPSTAADYAYILSLIKGAEEYNTA
                     PEGTVSFDTVGVKALDDYTIQYTFKDPGVFNINILSMWMIMAQPSWIIEGDACNEKLG
                     EKWVETGNYQSYGPFTLKEWVHDSNLTLIKNPYWPGTESVPQAKLPGVHIAILEDSAA
                     LAEFEAGNMDVAKVPISDYDRIVSDPVLSKMAVEVPGDIGTEFLTLNPTVPPTDDVRV
                     RKALSMSVDKQAIVKTLKTGKVASIFIHPALSGAPHEEDFPELGIDYDPEGAKQLIQS
                     YCDEKGIEPKDITISFIYPTREDLKIYVEAIQFMWQNTLGINVNLKNTEWDVFKVERM
                     ESKENVYRSTWTNDYMDANNFTADMFLCGATIQKIADWPSKDCTDVSNPAYQDYAQTV
                     IAAGKETDPAKRAALYAHSEEIIVSQEVIIIP"
BASE COUNT         1052 a          770 c          844 g         1002 t
ORIGIN      
        1 tggtcaccga tccggttctg gcgcagatgc ttcagacatt tccgccagag aatctgaatt
       61 cggtcgtttt gaaattgccg gcagtcaaga ccgtaacatt taccgcaacc gccaccgaaa
      121 ccgaaaccgt tactcctacc gttacattaa ctcccatgcc gccgaccggc acatttacat
      181 cgataccgac tctaaccgta accaacactt ttaccgccgt tccgacggca accgttcgtc
      241 ccgcttcgcc gactccatgg ccatctccga ctccgtatca aattattatt gtcgttgtca
      301 cggcaacgcc taaaccgacc atgccggttt ggattacatc aacaccgatt cgttgggtga
      361 cggctactcc gatttattgg tatacggcga caccatattg gatttatcgg acagcgacac
      421 ccattcctta tgtgccgccg gtatggaact atccgacagc ctatacctat ccgacgaaaa
      481 gcggcggaaa tacctatccg atttggactc cttatactcc gaaagcctca agcactccga
      541 aaccaaccgt gaacccggct tctttattcg aatttactta tgttacggaa aagatgtcgg
      601 cggataatat tccggtgaaa tggaactaca ccggtatgaa agagtatatg atcaattatt
      661 tgactgcgga tcgcacttta tatcccggat ttgcgatgga atggacagaa gcttcgaaag
      721 tgtgtaatgc ctctgcgtgt actttcgaaa taaaatcaat tcccgatcag cttttgaaac
      781 agggttcatt ctatatacaa cttcaggcga aagattcaaa cggaagaatt tatcaaagcg
      841 atccgatcgg tttgcaggtg gccggacaaa caacgcccca ggttaccggt acaccgaagt
      901 cagaagaacc tgaaaagaag ccgaatttta ttgtaagatt cttccgatgg ctgttcggac
      961 cgattattcg attattcggc ggcaaataag ggatggattt tttcgtctat ctctcgaaat
     1021 tcctgccaac gctggtatat ccggccggta ttttgtttat cagcctaatt ctggcgttgg
     1081 ctttttccaa acatccaaaa tggcagaaaa ggatggtcat ttttgcatta attgtgttat
     1141 ttgtatgcgg aaatcccttt cctaccgcgc tattaaccag aaatctcgag aacagttttc
     1201 tcccttttga cggcgcccaa aaggcgaagg tcatcgttgt gctgggcggg gggacggaat
     1261 ccaaggaata tccgcgccaa acagtcgaaa tcagcggtgc cggcgatcgg attctttatg
     1321 ccgcagatct ttggaaggaa ggatatgcgg aatatttgtt attcggcgga gcttattatg
     1381 agctattatc gggcgaaaaa cgatcggtcg cttcggatat ggcagaggtg gcggaatcgt
     1441 tgggaattcc gaaagataaa ataattctgc aagaaaaatc actgaacact tatgaggaag
     1501 cggtagaaga tgccgctgtt ctgaaaaaaa tgaatgtgga tgaagtaatc ttggtgactt
     1561 ctgccactca tatgcgacgg gcggcgggat gctttgaagc tcaagggctg aaggtgattc
     1621 cggctgccac cgattatggt tataccgata aatcgtggga aaatctgatg acgctcaatt
     1681 gggaaaaact atatacttac attattcctc aatcttctaa tatgagttct ctcgagacgg
     1741 ctttgaaaga atatatcggg attttcgttt atcgtctcag aggctggatg gagtgatgat
     1801 aaaaaacgtc tttattcctg tgagagagta gtttccgaat caatcattta cgaaaacacg
     1861 agattcctgt tttatccctc gacactgaaa aagaactgcc tgtggaacaa atcctattgt
     1921 tcatcatttt gtaattgaaa tcatcttaac tttttaaaaa agattattat caaaaaattc
     1981 ttacaaaatt ctgtattaaa aaatgtgtat gccgaaaggc gtttaattta taaatatcgg
     2041 aggaaagcta tgaaacagtt tcggactgcg ttaatcgtta tgattacctg tattgttttc
     2101 ttttcaacgg catctttggt cgctgctgat gagaccggct atttacgtgt agcgtatcca
     2161 agcacgtccc tttcaacgat cgatcaggca ttggtcagcg ataatagcca atatgtcgaa
     2221 tcaatgaatg tcggtgtcct gaggcaaaat gaattctcgg gagccttgga attcggtatg
     2281 gctaccgatc aacaaatgtc ggaagatgga acggttttca cctataaatt gttagagaat
     2341 gttccctggg tacgatacaa tccggctacg gatcaagtgg aaaaggtatt ggattgtgac
     2401 ggaaatgtaa gaatggtaaa agctcaggat tttgtctatg cggttgaaag agtgctccgc
     2461 ccatctactg ctgcggacta cgcttatatc ctgagcctga tcaaaggggc ggaagaatat
     2521 aatacggctc ccgaaggaac cgtttctttt gatacggtag gggtgaaagc gttagacgat
     2581 tatacgatcc aatacacgtt caaagatcca ggggtattta atatcaacat tctttcgatg
     2641 tggatgatta tggctcaacc ttcatggatt atagaaggag atgcctgtaa tgagaaactg
     2701 ggagaaaaat gggtagaaac cggcaattat cagagttatg gaccttttac acttaaagaa
     2761 tgggtgcatg attccaatct gaccttgata aaaaatccct attggcccgg gactgaatcg
     2821 gttcctcaag caaaattacc cggtgttcat atcgcgattt tggaagattc agccgctttg
     2881 gctgaatttg aagccggcaa tatggatgtg gcaaaggtcc cgatttcgga ttatgatcgt
     2941 atcgtttcag accccgtgct ttcgaagatg gcggttgaag ttccgggtga tatcggtacg
     3001 gaatttttga ctcttaatcc gacggttccg ccgacggatg atgttcgtgt tcggaaagca
     3061 ttaagcatgt ctgttgataa gcaggctatt gtaaaaacat taaaaaccgg taaagtcgcc
     3121 tcgattttta ttcacccggc attatcgggt gcacctcatg aagaagattt cccggaactc
     3181 ggaatcgatt atgatcctga aggggcaaaa caattaattc aatcctattg tgacgaaaaa
     3241 ggaatcgaac cgaaggatat tacgatcagt ttcatttatc cgacgcgtga agatctcaaa
     3301 atctatgtgg aagccataca attcatgtgg caaaatacat taggaattaa tgtaaatcta
     3361 aaaaatacag aatgggatgt attcaaagtg gagcgcatgg aaagcaaaga aaacgtttat
     3421 cgctcaacgt ggaccaacga ttacatggat gccaataatt tcacggcaga tatgttcttg
     3481 tgcggagcga cgattcagaa aattgccgat tggccttcca aagattgtac ggatgtcagt
     3541 aatcccgcct atcaggatta tgctcaaacc gttattgctg ccgggaagga gaccgatccg
     3601 gcaaaacggg ctgcccttta cgctcattcg gaagaaataa tcgtctcgca agaagttatc
     3661 atcattcc
//
LOCUS       DAOMIV010000174         3655 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_26560_length_3655_cov_3.334167, whole genome shotgun sequence.
ACCESSION   DAOMIV010000174 DAOMIV010000000
VERSION     DAOMIV010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26560_length_3655_cov_3.334167"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKV59_05085"
     CDS             complement(<1..331)
                     /locus_tag="PKV59_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46160.1"
                     /translation="MTTMTIFISPKPFNREHIATIQWNAIQSWKQLGEDVDIVLIGED
                     IGVREAAEELGVRYIAEVKRNESGTPILSDIFTLVRDTSDSPLLAYVNADIILFPDFL
                     EQARRMME"
     gene            complement(328..1104)
                     /locus_tag="PKV59_05090"
     CDS             complement(328..1104)
                     /locus_tag="PKV59_05090"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HQP46161.1"
                     /translation="MKTTLIIQARMGSSRLPGKVMKNLSGKPMLEWVIKRGEQAKKID
                     ETVVATTTDPSDDAVADWCEVNSVPCYRGSTYDVLDRYYQTALWRGTDRIVRVTADCP
                     LIDPNVIDDVVSLYETTGADFAANRLPPPWHRTFPIGLDVEVVSFPLLERAWKEADKP
                     FEREHVMPWFYTPEGRFKVAILDHDPDCGMHRWTADTPDDFELLQEIFRRLPDPLHAS
                     WTEVLELVERFPELEQINETTHAKQVDAIDERILRDKKSL"
     gene            complement(1108..2241)
                     /locus_tag="PKV59_05095"
     CDS             complement(1108..2241)
                     /locus_tag="PKV59_05095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP46162.1"
                     /translation="MKILYFSRDYCPHDYRFLKAIHDIGHEVFFLRLENSGRNLESRE
                     LPEAVHEAEWRWREIPYDPRNADAILSDLKEIFDQIQPDVIHSGPLTDVSWLTAEADL
                     HPHTAMSWGFDLMHDIEADPRLKQNAAIALHQADWFLGDCYVERDTAIPLGLKKDHAT
                     IFPWGIDPKQFCKGNSTVRRELAEEDEFLLLSTRSLEPNYRVDITLSAFIAAAAKEPK
                     LKLAILADGSQFNLLRNIADAAPKEIRDRILWLGRKPNDQLIDYYRAADLYLSASITD
                     GSSVSLLEAMACEIPVLVSDIPGNLEWVEDGRTGFLFKTGDSAQMTDKILFCYQNRGE
                     MQNITASARRLIEEKADWEKNKYRLTEAYRNAVTVCNSRYDSK"
     gene            complement(2243..3310)
                     /locus_tag="PKV59_05100"
     CDS             complement(2243..3310)
                     /locus_tag="PKV59_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HQP46163.1"
                     /translation="MELKFGNQTVGENHPVYFIADIAANHDGDLNRAKMLIHLAKESG
                     ADAAKFQNFKAPKIVSDFGFKNLGGQKSHQAKWQKSVFEVYQDASVPDRWSEELKEAC
                     EKEGILYFSSPYDFESIDWLDRYIPVYKIGSGEIDWLESLERIASKQKPVLLATGAAD
                     MSDVTRAVETILKINPQLVLMQCNTNYTASLENFKYIHLRVLETYHRLWPDLILGLSD
                     HTPGHATVLGAVALGAKVIEKHFTDDNERIGPDHKFAMNPKSWEEMVLNTRQLEAALG
                     SPEKKINENEKETAVIQRRCLRAARDIKAGERLTRDMIDVLRPATPGSLKPYEISAVI
                     GKKVLSNLPYGKEITRQDLED"
     gene            complement(3322..>3655)
                     /locus_tag="PKV59_05105"
     CDS             complement(3322..>3655)
                     /locus_tag="PKV59_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HQP46164.1"
                     /translation="YDLFHIGHLTLLKNAKGLYHVFSPEFLNKYEFGVNIAGKFGFDP
                     NLIQPVSWQAGGLDAPRSPYLIMNTDKLREFLGESLPDQETCLNHFYEAEKSGLPELI
                     RSFRTDNS"
BASE COUNT          944 a          925 c          771 g         1015 t
ORIGIN      
        1 tttccatcat tcgtcgagct tgttcgagaa aatccgggaa gagaataata tccgcgttca
       61 cgtatgccaa aagtggagaa tccgaagtat ccctcacaag tgtgaaaata tcgctcagaa
      121 tcggcgtacc ggattcattg cgttttacct ccgcaatata tcgcactccc aattcttcgg
      181 ccgcttcccg aacgccgata tcttcgccga tcaaaacaat gtctacatcc tctcccaatt
      241 gtttccacga ttgaatggca ttccattgaa tcgttgcgat atgctcgcga ttaaacggtt
      301 tgggagagat aaaaatcgtc atggtcgtca taatgatttt ttatccctga ggattcgctc
      361 gtcgattgca tcaacctgtt tggcatgggt tgtttcgttt atctgttcca attcggggaa
      421 tcgctcaacc agttcaagca cttccgtcca ggatgcatgc aacggatcgg gtaaccgccg
      481 gaaaatttcc tgtaataatt cgaaatcatc cggcgtatcg gcagtccatc gatgcattcc
      541 gcaatccggg tcatgatcca gaatcgccac cttaaaacga ccttcggggg tataaaacca
      601 cggcatgaca tgttcgcgct caaaaggttt atccgcttct ttccacgctc tctctaataa
      661 aggaaacgaa accacttcga catccaatcc aatcggaaag gttcgatgcc aaggaggggg
      721 taaacgattc gccgcgaaat ccgctccggt cgtttcatat aacgagacga catcatcaat
      781 tacattcgga tcgatcaaag gacaatccgc ggtaacccgc acgatgcgat ccgtccctct
      841 ccaaagagcg gtttgataat aacgatcgag gacatcataa gtcgatcctc gataacaggg
      901 caccgagttg acttcacacc aatcggcgac ggcatcatcg gaaggatcgg tcgttgtggc
      961 gaccaccgtt tcgtcaattt tttttgcctg ttctccgcgc ttgataaccc attccagcat
     1021 cggcttaccg gataagtttt tcatcacttt cccgggtaaa cggctggatc ccattcgggc
     1081 ttgaatgatg agtgtcgtct tcatttgtta tttcgaatcg tatcggctgt tacagactgt
     1141 aacggcattc cggtaagctt ccgtcagccg atatttattc ttctcccaat ccgctttttc
     1201 ttcgatcagt cttcgtgcgg aagcggtaat attttgcatt tccccacgat tttgatagca
     1261 gaaaagaatt ttatcagtca tttgggcaga atcgcctgtt ttaaacaaaa aacctgtcct
     1321 tccatcctcc acccattcca aatttcccgg aatatccgaa accagaacgg gaatttcaca
     1381 tgccatcgct tctaataggg atacggaaga tccgtcggtg atcgatgcac ttaaataaag
     1441 atccgccgcc cgataataat cgatcagctg atcattcggt tttctgccga gccacaaaat
     1501 gcgatcccta atttctttgg gagcggcatc cgcaatattt cgtaataagt tgaattggct
     1561 gccatccgct aaaattgcca atttcagttt cggttctttt gcggcagcgg cgataaatgc
     1621 ggaaagtgta atatccaccc gataattcgg ttcaagggac cgtgtactca gcaacaggaa
     1681 ttcatcttct tcagccaatt cccgccgaac ggtactgttc cctttgcaaa attgcttggg
     1741 atcgattccc caagggaaaa tcgtcgcatg atcttttttc agtcccaaag ggattgccgt
     1801 atctcgttcg acataacagt cacccaggaa ccaatcagct tgatgcaggg caatcgcagc
     1861 gttttgtttc agtctcggat cggcttcgat atcatgcatc aggtcgaacc cccatgacat
     1921 cgccgtatgc ggatgaagat ctgcttcggc cgtcaaccat gaaacatctg ttaatggtcc
     1981 gctatgaata acatcgggtt ggatttggtc aaaaatctct ttcagatccg acaagatcgc
     2041 gtccgcattt cgaggatcat aagggatttc ccgccaccgc cattccgctt cgtgaaccgc
     2101 ctccggcaat tcacggctct ccaaatttct tcccgagttc tcgagccgaa gaaagaaaac
     2161 ctcatgaccg atatcatgaa tggcttttag aaaacgataa tcatgagggc aataatcccg
     2221 cgaaaaatag agaattttca agttaatctt ccaaatcctg cctggttatt tcttttccgt
     2281 aagggagatt ggatagcact tttttgccga tcactgcgga aatctcgtag ggtttcaagg
     2341 agccgggtgt cgcgggacgt aagacatcaa tcatatctcg agtgagccgt tcacctgctt
     2401 tgatatcccg agccgctctc aggcaacgtc gttgaataac ggctgtttcc ttttcgtttt
     2461 cattgatctt tttttccggt gaacccaacg cggcttccag ttgacgggtg ttgaggacca
     2521 tttcctccca ggatttcgga ttcatggcga atttatgatc cggaccgatg cgctcattat
     2581 cgtcggtaaa atgtttttca atgactttgg cgcctaatgc aactgctccc aaaaccgtcg
     2641 catgtcccgg cgtatgatcc gataaaccca aaattaaatc cggccaaagc cggtgatacg
     2701 tctccaaaac ccgaagatgt atatatttga agttctcgag actggcggtg taatttgtat
     2761 tgcattgcat caaaacaagc tgcggattga tcttcaaaat cgtctcgacc gcccgtgtga
     2821 catcgctcat atccgctgcg ccggttgcca acagtaccgg tttctgtttg gatgcaatcc
     2881 gctcgagcga ctcaagccag tctatttctc cggaaccgat cttatagaca gggatatatc
     2941 gatccagcca atcaatcgat tcaaaatcat aaggcgatga aaaatacaga atgccctctt
     3001 tttcacaggc ttccttcagt tcctcggacc accggtccgg aacggaagca tcttgataaa
     3061 cctcaaaaac acttttttgc cattttgctt ggtgggattt ctgtccgccg agatttttaa
     3121 atccgaaatc ggagacgatt ttcggcgctt tgaaattttg aaacttagcg gcatccgcac
     3181 ccgattcctt tgccaaatga atcagcattt tggcacggtt caaatctccg tcatgattcg
     3241 ctgcgatgtc cgcaataaaa tatacggggt gattctctcc aaccgtttga ttgccgaatt
     3301 tcaattccat aattcctctt tttagctgtt atccgtccga aaagatcgaa tcaattcggg
     3361 caaacctgat ttttcggctt cataaaaatg attcaaacag gtttcctggt ccggtaaact
     3421 ttctcccaaa aattcccgaa gtttgtcggt attcataatc agataaggag atcgcggtgc
     3481 atccaacccg cccgcttgcc atgaaaccgg ttgaattaaa ttcggatcaa aaccgaactt
     3541 cccggcaatg tttactccga attcgtattt gttgagaaat tccgggctaa aaacatgata
     3601 gaggcccttt gcattcttaa gcagggtcaa atggccaata tgaaacaaat catat
//
LOCUS       DAOMIV010000175         3639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_26724_length_3639_cov_5.633929, whole genome shotgun sequence.
ACCESSION   DAOMIV010000175 DAOMIV010000000
VERSION     DAOMIV010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26724_length_3639_cov_5.633929"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..968)
                     /gene="cmr4"
                     /locus_tag="PKV59_05110"
     CDS             complement(102..968)
                     /gene="cmr4"
                     /locus_tag="PKV59_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HQP46165.1"
                     /translation="MYTYQAYLIETLTNLHVGSGGEQIGIVDNQIQRDPVTGFPSIYA
                     SSLKGSLRDHVKKNSNDSDKNTVIDIFGGSLDLEADEKKKQRTLEPGKVVFFDAHLLT
                     LPFRSSQKVYYNCTCPEILDNFTEQVETFNIPIKQTGKPSFGNDTSDFRVFDLVNPNL
                     EIEDFSVGNLTPGSENIDSFLTPVAGIIKTNFALFQNNIFTEICKTGLPVIARNSLDE
                     NGISQNLWYEEFLPRRTRLWFILGFPEDYENKEWLGERICADNIVQIGANFSIGYGYC
                     RIHKLPFPEVQS"
     gene            complement(970..2115)
                     /locus_tag="PKV59_05115"
     CDS             complement(970..2115)
                     /locus_tag="PKV59_05115"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HQP46166.1"
                     /translation="MQYLITFKPYSTFFFGTFKSFGEGYYAESSRFPLQTSIVGCLRK
                     TILKQNGWLDQKGNYPDKKYRNQYEQITGSVATVSMDSSDLNLGIIEKVSPVFIVRQD
                     PGHFCPDDFLFPVPQDIDYRYDPVEKDEEGNKLIIGFDYLAYQNESNAEEGSEIVTTK
                     IRNPKNDPPDYLGDRDFWEKYIRHEEIPYHPGCESGNIFLNVVQQGIARDENRQTVEG
                     AFYEKHAFCLAKGYSFGVIAVLNGETPFKKEKDDVFLGGDRSLFQMQIKPITAPYELR
                     YTGHPVMNRILGSEPHYEPPDDDNKYVCISALYGDQEPLDLEHALILSLEANRFLEYQ
                     KKKTDSYCGIPHSSVLYPKKGKSFIYKGYDMATKIGYNFIIKAKKGF"
     gene            complement(2118..>3639)
                     /gene="cas10"
                     /locus_tag="PKV59_05120"
     CDS             complement(2118..>3639)
                     /gene="cas10"
                     /locus_tag="PKV59_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HQP46167.1"
                     /translation="LERELQKNKKIEFLTPFIDNKENDRRAGLYPDRFVLESSLSPEE
                     IRNTIKTANEKALKHFIQIINNLGSLPAITFFKKKEIGNILREYLQTNMVVLPAEKIA
                     GKNVIETFWDYLDATEEHRHFKLGKSEKTCERCHTLLSVATAKVMDNNKKKDFDLCPL
                     CLLKLFTLEVVDVQNKISGWHRYPSTGEISAVELVEPFRKNDGTYQDELQNYFDKHDE
                     IEFDDIEFRQIVNKEIKPYHKYIAILDADGDKIGELAKKVKDPTAFSEMLFNFGKESY
                     EICSDYSRSGKGIGEPIYIGGDDVLAFLPVAYRKSEKDITTIIDLAMDLNKAFKVQMK
                     STEVGFSIGISIAYYKHPLAVTLNKSREMLKIAKAQDGKDTLAIHLKKHSGNDAQVVF
                     SFKESERLKEFNGLLKAVLLSEEDLMGSIHYNLLRFKNLLIQIETKEQLTNFFDNNFS
                     DQKKAGYTGIDHVQNMFEMALGLNSVAIPANSREKAVNEVLDQLWFIKFLVGKEEE"
BASE COUNT          987 a          777 c          688 g         1187 t
ORIGIN      
        1 ctcgtcattc gataatttat ccggatgctt taatttttca cttcgccatt gctgggttat
       61 ttcaatggcg atcggaatcc agtcttcgat gtttttccgg ttcatgattg cacctccgga
      121 aaaggtagtt tatgaatccg gcaatatcca taaccgatgg agaaattggc tccgatctgg
      181 acaatattat cggcgcagat tctttccccc agccattctt tattttcata atcctcagga
      241 aatcctaaaa taaaccacag ccgggttctt cgtggcagga actcctcata ccacagattc
      301 tgactgatcc cgttttcatc caggctgtta cgggcgataa ccggcaaacc ggttttacag
      361 atttcggtaa aaatattatt ctgaaaaagc gcaaaattag ttttgattat tccggcaact
      421 ggcgttaaaa aactatctat attttcacta cccggagtca gatttccgac actgaaatct
      481 tctatctcca aattcggatt aaccaaatcg aacacccgaa aatccgatgt gtcattcccg
      541 aaagatggct tcccggtttg ttttatcgga atattaaaag tttccacctg ctcggtaaaa
      601 ttatccagga tttccggaca ggtgcagtta taatatactt tttgagaaga ccggaaaggc
      661 aaggttaaca gatgcgcatc gaaaaaaacg actttgccgg gttcaagagt gcgttgcttc
      721 tttttttcgt ctgcctcaag gtctaaagag cccccgaaaa tatcaataac agtgtttttg
      781 tcagagtcat tagaattctt cttaacatga tctctgagcg agccttttag actcgaagca
      841 tatattgatg gaaaaccagt aaccggatcg cgctgaattt ggttatccac aataccgatc
      901 tgttccccgc cgctaccgac gtgcaggttg gtaagggttt caatgagata agcttgatat
      961 gtgtacatat taaaatcctt tcttcgcttt tattataaaa ttatatccta tcttcgtagc
     1021 catatcgtag cctttataaa taaatgattt tcctttttta ggatatagaa ccgaactatg
     1081 aggaataccg cagtaggaat cggttttctt tttttgatat tcaagaaaac gattggcttc
     1141 cagagaaagg atcaacgcat gttctaaatc cagcggttcc tgatctccat acaaagccga
     1201 gatacagaca tatttattat cgtcgtcagg tggttcatag tgtggttctg atcccagaat
     1261 ccgattcata accggatgac cagtatatct tagttcataa ggagcggtaa tcggcttaat
     1321 ttgcatttgg aacaacgatc ggtcgccgcc caaaaagaca tcgtcttttt ctttcttaaa
     1381 tggggtttct ccgttaagca cggcaatcac tccaaaagaa tagcctttcg ccagacagaa
     1441 ggcatgtttt tcatagaagg caccctcaac agtttgccgg ttctcatcac gggcaatgcc
     1501 ctgctgaacg acatttaaaa agatattgcc cgattcacaa ccgggatgat aaggtatctc
     1561 ttcatgacga atatactttt cccagaaatc ccggtcacca agataatcag gcggatcgtt
     1621 tttcggattg cggatcttcg ttgtgactat ttctgatcct tcttctgcat tcgattcatt
     1681 ttggtatgcc aggtaatcaa aaccaataat taatttgttt ccctcttcat ccttttcgac
     1741 tggatcgtag cgataatcaa tatcttgtgg aaccggaaac agaaagtcgt ccgggcagaa
     1801 atgtccgggg tcctgccgaa cgataaagac gggtgagact ttttcaataa ttcccaaatt
     1861 cagatcagac gaatccatag aaactgttgc aacactcccg gttatttgtt catattgatt
     1921 tcggtatttc ttgtccgggt agttgccttt ctgatccaac cacccgtttt gtttcaggat
     1981 agtttttcgc aggcaaccga cgatactggt ctggagtgga aaccggctcg attcggcata
     2041 ataaccttcg ccaaatgatt tgaacgtccc gaagaaaaag gtactgtacg gcttgaaagt
     2101 aatcagatat tgcattttca ttcctcctcc ttgccgacta aaaacttgat aaaccaaagt
     2161 tgatcaagta cttcattgac agctttttca cgactgttcg ctgggatagc gacactattt
     2221 agtcccagcg ccatttcgaa catgttttga acatggtcaa ttccggtata acctgctttt
     2281 ttctggtcac tgaaattatt atcgaagaaa ttcgttaact gttccttcgt ttcgatctgg
     2341 atcagcagat ttttaaacct cagcagattg tagtgaatcg aacccatcag gtcttcttca
     2401 gaaagtaata ctgcttttaa taatccatta aattctttga gtctttcgga ttccttaaat
     2461 gaaaacacta cttgcgcgtc attgccggag tgttttttaa ggtggatagc cagtgtatcc
     2521 ttgccatcct gcgctttagc gatctttaac atctcccgcg acttattgag tgtaacagcc
     2581 agcgggtgtt tgtaataggc gatgctgatg ccgatgctaa aaccaacctc tgttgatttc
     2641 atttgtactt taaaggcttt attcagatcc attgcaagat caattatagt cgtaatatcc
     2701 ttttcagatt ttcgatatgc aactggcaaa aatgccagga catcgtcgcc accgatatag
     2761 atcggctcgc ctattccctt tcctgatctt gaataatctg aacagatttc ataagactct
     2821 ttcccaaagt tgaataacat ttctgaaaat gctgttggat cttttacttt ttttgctaat
     2881 tcacctattt tatcgccatc ggcatccaga attgcaatat atttatgata gggtttgatt
     2941 tccttattaa cgatttgtct aaattcaata tcgtcaaact caatttcatc atgcttgtca
     3001 aagtaatttt gcaattcatc ttgatatgta ccgtcatttt ttcgaaaagg ttcaaccagt
     3061 tccaccgcac tgatttcacc ggtagatgga tatcgatgcc atcccgagat tttattctga
     3121 acgtcaacaa cctctaaagt aaataacttt agcagacata gcggacataa atcgaagtct
     3181 ttttttttgt tattatccat caccttcgca gtagcgactg atagtagggt atgacatcgc
     3241 tcgcaggttt tttcactttt accgagtttg aaatggcgat gttcttcggt tgcgtctaag
     3301 taatcccaaa atgtctcaat gacatttttt ccggctatct tttcagccgg aaggacaacc
     3361 atattggttt ggagatattc ccgcagaata ttgccgattt cctttttttt gaaaaaagtt
     3421 atcgcaggca gactaccgag gttgtttata atttgaataa aatgtttgag agctttttca
     3481 ttggccgttt taattgtatt tcgtatttct tccggtgata atgatgattc caatacaaac
     3541 cggtccgggt agagtccggc tctgcggtca ttttctttgt tatcaatgaa tggagtcaga
     3601 aattcaattt ttttattctt ttgtaattct ctttctaac
//
LOCUS       DAOMIV010000176         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_26844_length_3627_cov_4.764278, whole genome shotgun sequence.
ACCESSION   DAOMIV010000176 DAOMIV010000000
VERSION     DAOMIV010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26844_length_3627_cov_4.764278"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PKV59_05125"
     CDS             <1..694
                     /locus_tag="PKV59_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46168.1"
                     /translation="NDLKGKIISFGFDKPAENNGDPVIYCENMAIKREMNGEIENLIR
                     LDQIRLRGRHNIANTMAAFAVSTAAGFGIEAMHSAVVNFTGVEHRLQYVRNFHGADWY
                     NDSIATAPERTLAAVRSFDEPLVMLLGGRDKKLPWDELATEIHHHAHAAILFGEAAPL
                     IKKALERNKTSESELEIIQVDSLEEAVDAAASVCREGDVVLLSPGGTSYDAFRDFEER
                     GKAFTEWVMRLT"
     gene            673..1911
                     /locus_tag="PKV59_05130"
     CDS             673..1911
                     /locus_tag="PKV59_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HQP46169.1"
                     /translation="MGNAADVEFLKTKQARVSTKLLIDVPILVTVIILLILGLLFLYS
                     ASWNFALQEYDNAGYIVIRQMIWVILGLVLATILTFMDYHFIRNIAQLIILGTIFLLT
                     LVLFIPSSIQGATRTVLNGSVQPSELAKIALIIYLAYWLSNKQDKLSTLVWGFFPVIS
                     IIGIISVLVLLQPDVSATITIIFLGAVMFYVAGGNPKHLGVILLLALFIFLCGYFLFD
                     KVSTRINDYVNGLKDPSLASYHIQRSTEAILRGGLTGVGLGKGVVKLTGLPVAWTDSI
                     FTVILEETGLLGGTFIILLYLVLIWRGYEISVNAPDTFGKLLAAGVTLWIGIEAFINI
                     GVMVNIVPFAGNALPLISSGGSSLICTLAGIGILMSVSRSSAIEALKKERKTPDALID
                     LRGGNGGWRISSLGRGSRKR"
     gene            1835..2938
                     /gene="murG"
                     /locus_tag="PKV59_05135"
     CDS             1835..2938
                     /gene="murG"
                     /locus_tag="PKV59_05135"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQP46170.1"
                     /translation="MRLLICAGGTGGGVYPALAVVQERDKNHSDAILWVGSEKGMEYS
                     LVKHAGLEFRGIAAAGIHGVGWKKLPKNIASLVRGFFDSRRILKDFKPDVLFFTGGYI
                     GIPMAFAGIHIPQLIFIPDIEPGLSLKVLARLADTIAVVCDDSKKYLPSHKKIAVTGY
                     PTRAELRKWTKENARNLFGLSDLKPVLFIFGGSKGARSINQAVANHIHELLNLCQIIH
                     ITGQSDWDYVCKVREHLDPAEAKDYHINPYLHEEMGAAFAAADLVISRAGASTLGEFP
                     LFGLPAILVPYPYAWRYQKVNADYLAEKGAAIIIEDAMLDKEIVGTVGDLMQNRDKLA
                     EMKKAMSSLAIPDAAKNIAVLLEELAISKSEQS"
     gene            2941..>3627
                     /locus_tag="PKV59_05140"
     CDS             2941..>3627
                     /locus_tag="PKV59_05140"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HQP46171.1"
                     /translation="MKIHDGMKIHIIGIGGTGMSAIALVLAEAGAIVTGSDRSRSDIV
                     SKLEDAGIKVFLGHRSENIGNVDLVIYSSAIPKDNPELREAQRRGIPVKRRIEFLNDL
                     LEDRDVIAVAGTHGKTTTTAMIAWLFSESGSEPGFIIGSTPKNLGKNAALGKSKEFVI
                     EADEYDNMFLGLFPSVAILTRVEHDHPDCFPTMDLYLNAFRKFISQVKPGGIILMNAD
                     DPNQQLLKDKM"
BASE COUNT          955 a          762 c          932 g          978 t
ORIGIN      
        1 aaatgatctg aaaggaaaaa ttatcagctt cggatttgat aaaccggcag aaaacaacgg
       61 cgatccggtt atctattgcg aaaatatggc gatcaaacga gagatgaacg gtgaaattga
      121 aaatctgatc cgcttggatc aaattcgatt gcggggaagg cacaatattg ccaatacaat
      181 ggcggctttt gccgtatcta ccgcagccgg attcggtatc gaagcgatgc attccgccgt
      241 cgtaaacttt accggcgtgg aacatcgttt gcaatacgta cggaattttc acggagccga
      301 ttggtataac gattccatcg ctaccgcacc ggaacgaact ttggctgcgg tgagaagttt
      361 tgatgaaccg ttagtgatgc ttctgggagg acgagataaa aaattaccgt gggacgaatt
      421 ggcaaccgaa attcatcatc atgcacatgc ggcgatattg ttcggagaag cggcaccttt
      481 aatcaaaaag gcgttggaac ggaacaagac ttccgagtcg gaattggaga ttattcaggt
      541 cgattcgctc gaagaagcgg tagatgctgc cgcatctgtt tgtcgggaag gggatgtagt
      601 ccttctgtct ccaggaggga ccagttatga cgctttccgt gattttgaag aaagaggaaa
      661 ggcttttacc gaatgggtaa tgcggctgac gtagaatttc taaaaaccaa gcaagctcgt
      721 gtctcaacca agctgctgat cgacgttccg attttggtaa cggttattat tttattgatt
      781 ctgggcttgt tatttctgta ttccgcgagt tggaactttg cattgcagga atatgataat
      841 gccggttata tcgtcatccg acagatgatc tgggtgatat tgggattggt tctggccacc
      901 attctgacct tcatggatta ccattttatt agaaatattg cccagctgat tattctgggg
      961 acaatatttc tgctgacgtt ggttcttttt atcccatcca gtattcaggg tgctacccgc
     1021 accgtgttaa acggctctgt tcaaccttcc gaactggcaa aaatcgctct gattatttat
     1081 cttgcatatt ggttaagcaa taagcaggat aaactgagca cgcttgtttg gggatttttt
     1141 ccggttatct ccatcattgg gattatttcg gtactcgttc tcttacaacc ggatgtcagc
     1201 gcaacaatca cgattatttt cctcggtgcg gttatgtttt atgtcgcggg agggaacccc
     1261 aaacatttgg gagtgattct gctgcttgcc ctttttatat tcttatgcgg ttacttcctt
     1321 tttgataaag tcagtacgcg catcaatgat tatgtgaacg ggctgaaaga tccgagtttg
     1381 gcttcttatc atattcaacg atcgactgaa gccatcctga ggggaggttt aaccggagtc
     1441 ggtttaggga aaggagtcgt gaaactgacc ggattaccgg tggcttggac ggacagtatc
     1501 ttcaccgtaa ttttggaaga aaccggctta ttgggcggca cttttatcat tcttctgtat
     1561 ttagtcttga tttggcgcgg ttatgaaata tccgtgaatg caccggatac attcgggaaa
     1621 ttactggcag cgggagtaac gctttggatc ggtattgaag cctttattaa tatcggagtc
     1681 atggtgaata tcgttccctt cgcagggaat gcattgccgc tgatcagcag cggcggatca
     1741 agtcttatct gtacgttggc gggaatcgga atactgatga gtgtttccag atcgtcagcc
     1801 atcgaagctt tgaaaaaaga aaggaaaaca ccggatgcgc ttattgatct gcgcgggggg
     1861 aacggggggt ggcgtatatc cagccttggc cgtggttcaa gaaagagata aaaaccatag
     1921 cgacgccatt ctctgggtcg gatccgaaaa aggtatggaa tattcactcg taaaacacgc
     1981 cggattggaa ttccgcggaa ttgccgccgc cggaatacat ggtgtcggct ggaaaaagct
     2041 gccgaaaaat atagccagtc tggtccgtgg tttcttcgat tcccgccgga ttctgaaaga
     2101 ttttaaaccc gatgtactct tctttaccgg cggttatatc ggcattccga tggctttcgc
     2161 cggaattcat attccgcagc tgatttttat tccggatatt gaacccggtt tgtcattgaa
     2221 agttctggca agactggctg atacgatcgc agtggtgtgc gacgattcaa aaaaatattt
     2281 gccttcccat aaaaagattg cggttacggg atatccgact cgagccgagt tgaggaaatg
     2341 gacgaaggaa aacgccagga atttgttcgg cctgtccgat ctgaaaccgg ttttgttcat
     2401 tttcggcgga agcaaaggag cgcgctctat caatcaggcg gtcgcaaacc atattcatga
     2461 actgctgaac ttatgccaga ttatccatat caccgggcaa tcggattggg actatgtttg
     2521 taaggttcgt gaacatctgg atccggcaga agcaaaggac taccacatca atccatacct
     2581 gcatgaagaa atgggagccg cgtttgccgc agccgatttg gtcatttccc gtgcgggcgc
     2641 ttccactttg ggagaattcc ccttgtttgg attgccggcg attttagtcc cctaccccta
     2701 tgcttggcgt tatcaaaaag tgaacgccga ttatttagcg gaaaaagggg cggcaataat
     2761 aattgaagat gccatgctgg ataaggaaat cgtcggtacc gtcggagact tgatgcaaaa
     2821 tcgggataaa ttagcggaaa tgaaaaaagc aatgagcagt ttggcgattc cggatgctgc
     2881 aaaaaatatc gctgttttgc tggaagaatt ggccatatca aagagtgaac agagttgaag
     2941 atgaaaatac atgacgggat gaagattcat attatcggca tcggcggaac cggaatgtca
     3001 gccatcgcat tggtactggc agaagccgga gcaattgtca ccggctccga ccgttctcgt
     3061 tccgatattg tgtcgaaatt ggaagatgcc ggaatcaaag tttttctcgg acaccggtcg
     3121 gaaaatatcg ggaatgtcga tctcgtcatc tattcttccg cgattcccaa ggataacccg
     3181 gaactgcggg aagcgcaacg aagaggtatc cccgtcaaac gtcgtatcga attcttaaat
     3241 gatctgttgg aggatcggga tgtgatcgct gttgccggga cacacgggaa aacaaccacc
     3301 accgctatga tcgcttggct gttcagcgaa tcgggatcgg aacccggatt tattatcgga
     3361 agcaccccga aaaatctggg gaagaatgcg gctttgggga agagcaaaga atttgtgatc
     3421 gaagccgatg aatacgacaa tatgttcctc gggctctttc cttccgtcgc aatattaacc
     3481 cgcgtcgaac atgaccatcc ggattgcttc ccgactatgg atctgtattt gaatgccttc
     3541 cgaaaattta tctctcaagt caagcccggc ggaattattc tgatgaatgc cgatgatccg
     3601 aatcagcaat tattaaaaga caaaatg
//
LOCUS       DAOMIV010000177         3625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_26877_length_3625_cov_4.480952, whole genome shotgun sequence.
ACCESSION   DAOMIV010000177 DAOMIV010000000
VERSION     DAOMIV010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26877_length_3625_cov_4.480952"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKV59_05145"
     CDS             <1..887
                     /locus_tag="PKV59_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQP46172.1"
                     /translation="YVIGPLTEKRDGTQVPYVMPDRCPSCGQPLEHLENEVAVYCVNN
                     ACPAQLVRNVEHFASREAMDINGLGIKIVEQIVQNGLIKDVGDLYSLKKEDLLSLEGF
                     GDKKAENLITAIENSKKQPLSRLINALGIRGVGEVMAITLAEHFPSLDQLKNATADDL
                     MQIEGIGPNVAQAILDWFSRDTNQMVLKKLQNAGVWPVNMEAAESDKAPQVLAGKTIV
                     VTGTLSHFTRDEIKDFILKHGGKPVESVSNKTSFVIVGENPGSKRDKAIELGIPLYTE
                     QMFLDMVGIEKNGQRTID"
     gene            1007..1255
                     /locus_tag="PKV59_05150"
     CDS             1007..1255
                     /locus_tag="PKV59_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HQP46173.1"
                     /translation="MGLVSWLVVGGLAGWIASKIMGKDAKMGLMANIAVGIIGALVGG
                     FVLQIFGIGGVTGINLRSVLVAILGSCILLYIMNKINR"
     gene            complement(1353..1733)
                     /locus_tag="PKV59_05155"
     CDS             complement(1353..1733)
                     /locus_tag="PKV59_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HQP46174.1"
                     /translation="MEQKFLYCKHCGKVLIPGVDRDTPTICCGEEMVILKANSTDAAQ
                     EKHVPVVKFTDNKIEVAVGSVLHPMTPEHFIQFIYLQTEKGGQLKKLNPGDEPKAVFL
                     IGEDKPLVVYEFCNLHGLWKADVI"
     gene            complement(1807..>3625)
                     /locus_tag="PKV59_05160"
     CDS             complement(1807..>3625)
                     /locus_tag="PKV59_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HQP46175.1"
                     /translation="YIDPVQVNENGVLVEKGGFARIKTIIDSVKTQNENTFVLDGGDF
                     SMGTLYQSVFSEKALELRLLGLMGYQATTIGNHEFDYRSDGLAKMLDAAKASNDPLPI
                     IVQSNINWEASQGEYTEMLHKSMENYGVKKYAIIQAGDLKAAVFGLMGKEAASYAPTS
                     GLIFDDIVESSKEIVNEIKKNENVDLIIALSHSGTNSDSKSSEDEILAKEVPEIDVII
                     SGHSHTYMDNPILIGDTAVVSAGEYGRFVGNLKLSKKENNRWKVDQFTPIPVDESIEP
                     DPDLQKTIDHYRESLNSYIQLFGFDSYDQIIARNPYPFDSVQALYKDPVDNPLGNLIS
                     DSYVYEIKKVEGENYIPVDVAIVPVGIIRSTLNQGDIHVRDAYEILSLGIGEDGISGY
                     PLINVYLTGAELRMVAEIDASLSDLMSGTKLYWSGLYSEFNPHRMILNRVVDVKLIGE
                     DGSLKDLDDKKLYRVVAGLYSGQMLGAVESMSKGLLQLKPKDKDGNPLEGFSSVIIKN
                     QSGMEVKEWYAFADYLSSFEKIDGISTIPAKYSKSEGRKVINDSTGIKDILRNPSAPF
                     LKVTGILLLAILIIVLIIILLVKLIRRITRKQSNKIAEK"
BASE COUNT         1074 a          723 c          746 g         1082 t
ORIGIN      
        1 cctatgtaat cggacctctg acggaaaaaa gggacgggac tcaggttcct tatgttatgc
       61 ctgatcgatg tccttcctgc ggacaacctt tggaacatct tgaaaatgaa gttgccgttt
      121 attgtgtcaa taatgcctgt ccggctcaat tggtgcgaaa tgtagagcat tttgcttcac
      181 gcgaagcaat ggacatcaac ggattgggca taaagattgt cgaacagata gtgcaaaacg
      241 gattgataaa agatgtgggc gatctctaca gcctaaaaaa agaagatttg ctttccctcg
      301 aaggattcgg agataagaaa gcggaaaatc tgatcacggc gattgaaaat tcaaaaaaac
      361 aacctttgtc acgattaatc aatgcattgg gaattcgagg agttggagaa gtgatggcaa
      421 ttacattggc tgaacatttt ccttcattag atcaattaaa aaatgccaca gccgatgatc
      481 tgatgcaaat cgaagggatc ggtccgaatg tcgctcaagc catcttggat tggttttcgc
      541 gtgatacaaa tcagatggtt ttaaaaaaat tacagaacgc aggtgtatgg cctgtcaata
      601 tggaagccgc tgaatccgat aaggctccac aagttttagc gggaaaaaca attgtcgtaa
      661 caggaacttt atctcatttt acccgcgatg aaataaaaga ttttattttg aaacacggag
      721 gaaaaccggt tgaaagtgtc agcaataaaa cttctttcgt gattgtcggc gaaaatcccg
      781 gatcgaaacg ggataaagca atcgaattgg ggataccgct gtataccgag cagatgtttc
      841 tcgatatggt cggaatagag aaaaatggtc aaaggacgat tgactgatgc ttttcgaaat
      901 atcatataaa aaaataatca tttgatcgaa taaattgtgt tacgataaat tacaaataat
      961 acggaatcgt gccgagtata tttaattcaa cgaaagagga ggaactatgg gacttgtttc
     1021 atggttggtt gtcggaggct tagccggttg gatcgccagt aagattatgg ggaaagatgc
     1081 caaaatggga ttgatggcta atattgcagt aggaattatc ggcgcattag tcggtggatt
     1141 tgttctgcaa atttttggaa tcggcggtgt aacgggcatt aatctgagaa gtgttttggt
     1201 agcgattttg gggtcttgta tattgcttta tattatgaac aaaataaaca gatagatttt
     1261 atcttttcaa tggaaaagac ttctactatt aaaaagcacc gcttccattc ggagcggtgc
     1321 tttttttaaa gattgattca atatgaaaag aattatatga cgtcagcttt ccatagacca
     1381 tgaaggttgc agaattcata aacaaccaac ggcttatctt caccgatgag gaaaaccgct
     1441 ttcggctcat cgccgggatt taatttcttc agttggccgc ctttctccgt ctgcaaatag
     1501 ataaattgga taaaatgttc gggagtcatc ggatgtaaaa cacttccgac ggcgacttcg
     1561 attttattat ctgtgaactt gacaacgggg acatgttttt cttgagcagc atcggtacta
     1621 tttgctttca gaatcaccat ttcttccccg cagcatattg tcggagtatc tcggtcaact
     1681 cccgggataa gaacttttcc acaatgttta caatataaga atttttgttc catatttccc
     1741 tcctcaaacg aaatgatttg gtatataaaa ttcaaccaat attaagtata tcaaaaattt
     1801 ccgatcttac ttttctgcga ttttatttga ctgtttgcgg gttattctcc gaatcagttt
     1861 tactaagaga atgatgatta aaacaatgat gagaatggcg agcagcaaaa tccctgtgac
     1921 tttcaggaac ggagcgctcg gatttcgtaa aatatccttg ataccggtac tgtcattgat
     1981 aacttttcga ccttcgctct tggaatattt ggcgggaatt gtcgaaatcc catctatttt
     2041 ttcaaaggaa ctcaaataat cggcaaaagc ataccattcc tttacttcca ttcccgattg
     2101 atttttaatg attacggaac tgaaaccttc caaaggatta ccgtctttat ctttcggttt
     2161 tagctgcaat aaaccttttg acatggattc gacggctccc agcatttgac cggaatacaa
     2221 tccggcgacc acacgataaa gtttcttgtc atccaaatct ttcagtgaac catcttctcc
     2281 gattaattta acatccacta ctcgattcaa tatcatccgg tggggattga attcgctgta
     2341 aaggccgctc caataaagtt tggtaccact catcagatca gagagcgatg catcgatttc
     2401 cgctaccatt cgaagctctg cgccggtcag gtaaacgttg atcagcgggt atccggaaat
     2461 accgtcttcc cctattccca gtgataatat ctcataagca tctcgaacgt ggatatcccc
     2521 ttgatttaat gtcgaacgaa taattccgac cggaacgatc gcaacatcaa ccgggatata
     2581 attttctcct tcaactttct ttatttcata aacataggaa tcggaaatca aatttccgag
     2641 agggttatca acgggatctt tgtataacgc ttgcaccgaa tcaaacgggt aaggatttct
     2701 ggcgattatt tggtcataac tgtcaaatcc aaaaagttgg atatatgaat tcaatgactc
     2761 acggtaatga tcgattgttt tttgcaggtc aggatcgggt tcaatacttt catcgaccgg
     2821 aataggagta aattgatcaa ctttccagcg attgttttcc tttttggaaa gtttaagatt
     2881 tccgacaaat cgaccatatt caccggctga aacaacagcg gtgtccccga tgagaatcgg
     2941 attgtccata taggtatgtg aatgtccgct gatgattaca tcaatttcag gaacttcttt
     3001 ggcaagaatt tcatcttccg aactcttgga atcggaattc gttccggagt gggacaaggc
     3061 aataatcaaa tccacatttt catttttctt aatttcattg acaatttcct tggaactttc
     3121 gacaatatca tcgaaaatta agccgctggt cggtgcgtaa ctggcggctt ctttacccat
     3181 caaaccaaac actgcggctt tcagatcacc ggcttgaatg atcgcgtatt ttttaacacc
     3241 gtaattctcc attgatttat gcagcatttc ggtatattca ccctgagaag cttcccaatt
     3301 gatattggat tgaacgatga tcggcaaagg atcattactg gctttggcag catccagcat
     3361 ttttgccagc ccgtcactgc gataatcaaa ttcatgattc ccgatggtag ttgcttgata
     3421 ccccattagc cccaataatc gtaattccaa cgctttctcg gaaaaaacag attggtaaag
     3481 ggtacccatc gaaaaatcgc cgccatccaa gacgaaggta ttttcatttt gagttttaac
     3541 cgaatcaata attgttttta tgcgagcaaa gccacccttt tcaacgagta ctccgttctc
     3601 attcacttgc acgggatcga tatag
//
LOCUS       DAOMIV010000178         3610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_27036_length_3610_cov_4.250352, whole genome shotgun sequence.
ACCESSION   DAOMIV010000178 DAOMIV010000000
VERSION     DAOMIV010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27036_length_3610_cov_4.250352"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PKV59_05165"
     CDS             <1..613
                     /locus_tag="PKV59_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46176.1"
                     /translation="FEDSQPEDAISEKPVEEVQIPYTESDIHPEEKNIGSDFSFKPES
                     GRKNETSETISKEDLSSEDEPLIHYQTIYKLGDDFFDETFSLDEGDKFIGECGIGIAE
                     TINNTDPKAVTAFEIWLFDREDSQTPTHFLLSDYAYNNDELLERLKNKGRFDLIERNR
                     DYEVKTQNLRMIVTIKDMEYGTESSDKKSYFNKVTFDVQVWKI"
     gene            complement(675..788)
                     /locus_tag="PKV59_05170"
     CDS             complement(675..788)
                     /locus_tag="PKV59_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HQP46177.1"
                     /translation="MSYQSPIGSALYQRKVNDVVIAETPNGKIKFKILEIK"
     gene            complement(797..1144)
                     /locus_tag="PKV59_05175"
     CDS             complement(797..1144)
                     /locus_tag="PKV59_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46178.1"
                     /translation="MPTETSYLTSEGAQKLQEELEFLKGPRRAELAKRLRFAIEQGDL
                     SENADYSAAKEEQAFVEGRIQELETILSNVKIIDDLKREKGVVNLGSTVRIRKKISSR
                     KNIKLSDRRKRIR"
     gene            complement(1156..2310)
                     /locus_tag="PKV59_05180"
     CDS             complement(1156..2310)
                     /locus_tag="PKV59_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HQP46179.1"
                     /translation="MNYASALNTFRKEEVAIAPYGIRSKDSKGRQFPEEDHAFRSCFQ
                     RDKDRIIHTTSFRRLEYKTQVFINDEGDYYRTRLTHTLEVAQIGRTLARALGGNEDLV
                     EAICLAHDMGHPPFGHSGESALAKMMVNSGSFNHNHQTLRIVTKLEKRYADFDGLNLC
                     YETLEGIAKHETEFNFSDLLEYNPDSRGHLEAQIANVADEIAYSAHDLDDGLRSGIIT
                     VDMCEDLLIWKTIAEEIHWVGPKLADLDRHRFINALINLAVTDTFYTSQEKITSAKIK
                     SVDELQRLPYNVVGNSESWLIQNRELKDFLFNNLYRHYRVVRMNLKADMIITRLFEAF
                     RKEPSLLPKHIQTTIEERGTDTAICDYIAGMTDRYAIEQYQKLFEPSLLP"
     gene            complement(2559..3362)
                     /locus_tag="PKV59_05185"
     CDS             complement(2559..3362)
                     /locus_tag="PKV59_05185"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HQP46180.1"
                     /translation="MITLLKLGGSLITNKEKPHSVRTETIRRVGFEIKEALDSDPDIK
                     LILGHGSGSFGHVPASRYHTLDGVQTSSEWRGFAEVHAEADELNKITVSILRGCGLPI
                     LGFSPLSSVFTRKRKVTRWNIDPIFQSILHGLIPLIYGDTVLDSALGGTILSTEELFA
                     ALAIKSPKPVRILLAGIEEGIWKDYQKKDSLIKEMDLSDPENTERDFIKQSAYTDVTG
                     GMFSKVQSMEELIRKNLIQEALIFSGEKQGNIKAALLGGTPGTKIFRSV"
     gene            complement(3359..>3610)
                     /locus_tag="PKV59_05190"
     CDS             complement(3359..>3610)
                     /locus_tag="PKV59_05190"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HQP46181.1"
                     /translation="ALEDGDIKLIGELMNKNHELLKRLTVSSPELDSLYEIAIEQGAI
                     GAKLTGSGRGGNIIALVEEEDQLAYLRQALQNKGAKVLN"
BASE COUNT         1004 a          803 c          743 g         1060 t
ORIGIN      
        1 ttttgaggat tcgcagccag aagacgcaat ttcggaaaaa cctgttgaag aagtgcaaat
       61 tccgtatacc gaatcggata ttcatccgga agaaaaaaac atcggttcgg atttttcgtt
      121 taagcccgaa agcggtagaa aaaacgaaac ttccgagacg atttcgaagg aagatctttc
      181 atccgaggat gaaccgctga ttcactatca gacgatttac aaactgggcg atgatttttt
      241 cgatgaaact ttttctctcg atgaaggaga taaattcatc ggagaatgcg gaatcggaat
      301 tgccgaaacg atcaacaata cggacccgaa agcggttacg gcttttgaga tctggctgtt
      361 tgatcgggaa gattctcaga ccccgactca ttttttgttg agtgattatg cttataataa
      421 cgatgaattg ttggaacgat tgaaaaataa gggcagattc gatctgatcg aacgaaatcg
      481 agattatgag gtgaagactc aaaatttgag gatgatcgta acgattaagg atatggaata
      541 cggaacggaa agcagcgata agaaaagcta ctttaataaa gtgacatttg acgtccaagt
      601 gtggaagata taaactaatc aaaaaagagc ctgcgtaaaa tgccgcaggc tcttttttct
      661 gttaaccaga ttgattattt gatttcgaga attttaaatt tgatctttcc attcggcgtt
      721 tcggcgatca cgacatcatt taccttccgt tggtaaagtg ccgatccgat gggtgactga
      781 taggaaatta aaccgttcag cggatccgct tcctgcggtc cgacaatttt atattcttcc
      841 ggctcgaaat cttcttccta attctcaccg tcgaacccaa gttgaccact cctttttccc
      901 gcttcaaatc gtcgataatt tttacgttag ataagattgt ttcaagctct tggattcttc
      961 cttcgacaaa agcttgttcc tcttttgctg ccgaataatc cgcattttca gagagatctc
     1021 cctgttcaat cgcaaaacgc aaccgtttgg caagttcagc ccgccggggt ccttttaaaa
     1081 attcgagttc ttcctgaagt ttttgagcac cttccgaagt taaataggat gtttctgtag
     1141 gcattttata ctcctttatg gcaggagaga gggttcaaat aatttctgat attgttcgat
     1201 agcatatcga tcagtcatcc ctgcgatgta gtcgcagatg gcggtgtctg ttcccctctc
     1261 ctcaattgta gtttgaatat gtttcggcaa aagtgaaggt tccttacgga aagcctcaaa
     1321 taacctggta atgatcatat ccgctttcaa attcattctg acaactcgat aatgacgata
     1381 caagttgtta aaaagaaaat ctttcagctc acggttttga atcaaccagg attcactatt
     1441 acccactaca ttataaggca aacgttgcaa ctcgtccact gatttaatct tcgcggaagt
     1501 aatcttttcc tgtgaggtat agaaggtgtc cgtgacagcc agattgatta atgcattaat
     1561 aaaacgatgc ctgtctaaat ctgccaattt tggacccacc cagtggattt cctccgcgat
     1621 tgttttccaa atcagcagat cctcgcacat atcaaccgtg atgattcccg agcgtaagcc
     1681 atcatccaaa tcatgagcgg aataagctat ttcatcggcg acattggcaa tttgagcctc
     1741 cagatgccct cgcgaatccg gattatattc cagcaggtcc gagaaattaa actccgtctc
     1801 atgcttggcg attccctcca aggtttcata acaaagattt aatccgtcaa aatcggcata
     1861 tcttttttcc agtttggtta cgatccggag cgtttgatga ttgtggttga aagagccgga
     1921 attgaccatc atttttgcca atgcagactc accggaatga ccgaaaggag ggtgccccat
     1981 gtcatgcgca aggcagatcg cttctaccaa atcttcattc cctccgagtg cccgagccaa
     2041 tgttcttccg atctgcgcga cttcaagtgt atgagtcaga cgagtacgat aataatcgcc
     2101 ttcatcattt ataaatacct gcgtcttata ttccaatcgt ctgaacgaag tagtatggat
     2161 gattcgatct ttgtcccgtt ggaagcaaga gcgaaaggca tgatcttctt cgggaaattg
     2221 ccgccctttt gaatcttttg aacggatccc gtagggcgcg attgcaacct cttcttttct
     2281 aaaagtattt aaagcagatg catagttcat tttcttaaac cctttatcga ttcatcgtcg
     2341 gttccggtca ttttatcgtc aatataccaa aaagcgatga actctgatca tacatgaatg
     2401 ttgttcatcc tataccggat tcacatttat tatcacttaa tgcttcttaa ttttgccacg
     2461 atttaaagga taaaccgaaa acttctcact ccggccggaa tgaaaggtat aaattcgtcg
     2521 aatcatcatt ccgcaaaaga acagatagaa ggtgccgatt aaaccgaccg aaagattttt
     2581 gttccgggcg ttcctcctaa aagagccgct ttgatatttc cttgtttttc tccggaaaat
     2641 attaaagctt cctgaattaa attctttctg attaattctt ccatcgattg aactttggag
     2701 aacatgccac ccgtcacatc ggtataagcg gattgtttaa taaagtctct ttccgtattc
     2761 tcgggatcgg aaagatccat ttctttgatt agagaatctt tcttttgata atctttccat
     2821 atgccttctt cgatacccgc caacaagatc cgtaccggtt tcggcgattt gatcgccagc
     2881 gccgcaaaaa gctcttcggt ggaaagaatg gtaccgccga gtgcggaatc aagaacggta
     2941 tccccgtaaa tgaggggaat taagccatgc aaaatggatt gaaaaatagg atcgatattc
     3001 catcgggtta cctttctttt ccgggtgaaa acagagctca acggcgaaaa gccgagaatc
     3061 ggcaatccac agcctctgag gatcgatacc gtaatcttgt tcaactcatc tgcttcggca
     3121 tggacttctg caaagccacg ccattcactc gaggtctgaa ccccgtcgag ggtatggtaa
     3181 cgagaagcag ggacatgacc gaatgaaccc gatccgtgtc ctaaaatcag tttaatatcc
     3241 ggatccgaat ctaaagcttc tttaatctcg aatccgactc ttcgaatggt ctctgtacgg
     3301 acggaatggg gtttctcttt atttgtaatt aacgaaccgc ctaatttcaa caatgtgatc
     3361 aatttaagac ctttgccccc ttgttttgca aagcttgtcg aagatatgcg agctgatctt
     3421 cttcttcaac caatgcgatg atattaccgc ctcgtccgga accggtaagt ttggcgccga
     3481 ttgccccttg ctctatggcg atttcgtata gggaatcaag ctccggggac gaaacagtta
     3541 atcgcttcag cagctcatgg tttttattca ttaactcacc gatcagtttg atatcaccgt
     3601 cctccaaagc
//
LOCUS       DAOMIV010000179         3595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_27208_length_3595_cov_3.428249, whole genome shotgun sequence.
ACCESSION   DAOMIV010000179 DAOMIV010000000
VERSION     DAOMIV010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27208_length_3595_cov_3.428249"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..951)
                     /locus_tag="PKV59_05195"
     CDS             complement(49..951)
                     /locus_tag="PKV59_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQP46182.1"
                     /translation="MNFKKITDSYHFYAIITILCWAVAPVLTRLCHRHFSAMPIGFLR
                     YLISSFLIILLLIIKKTKFPLIKDLPWLIASAATGFSLYMVMFNFGTQYVESATTSVM
                     MATSPLITALLSFFLFKETLSKNQWLAILIEFIGIIILTVVGNGFSLNIGTLWLFGAS
                     FLLSVYNLIQRILTKKYSGFQVTAFSILIGTLMLSFSAPQSFRELKTAPPNQIFYLLI
                     LGIFTSTIAYISWSTAFQKAEKTSQVSNYMFFNPLLSGILAFFIAGEIPDSTTYIGGV
                     LILLGMWLFNHGQAIQPKAKIHSH"
     gene            1320..2825
                     /locus_tag="PKV59_05200"
     CDS             1320..2825
                     /locus_tag="PKV59_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46183.1"
                     /translation="MKKNILYVVLFLLIFAVLPVNAAGDTLVIGVATDMTGMDPHQQT
                     AFTSMKVLEMMYGQLVEVNENMEVVPSMAESWEWSEDGMHLTMHLVPEMTFHDGSPVT
                     SADVKYSFERILNEETAAAARSKFTAISSIDTPDDLTVVFNFGEKNVAILVDMASTNA
                     AILSKAFMENGGDLSTTEMGSGPYMMKKWEPDNKIEMVAYEKYFMKGLPKIKNLEFRI
                     IPSEDSILAGIRTGEIDFAVINEPATAMIASKDKNVVVMEAPALSYHVLQLNPSREVF
                     KNVKVRQAIACAIDRQEVLDVASLGEGEVTPPAVQPFYKANLDDLFCYKKDLEKSKAL
                     LAEAGNPQVEFTIMAAADEPPTAVAEAQNIQDQLSEIGIKVNIEVLELGVYVDRWLAG
                     DFDACTALNGGGSDPNGTFWRYWHSTGNLQKVSGYSSPELDKLLEEGRSYTDPEVRKG
                     YYLEVQKNLAENAPWVWLYVGYEYRVMVPSLKGFVPTPNGSLWSLRQVYFE"
     gene            2925..>3595
                     /locus_tag="PKV59_05205"
     CDS             2925..>3595
                     /locus_tag="PKV59_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46184.1"
                     /translation="MRNYIFRRLLETIPTLLGISIAIFLIMHMIPGDTISALIGTQYQ
                     LTEAQAESLRAYYGLDKSLPDQYISWLLNALQGNFGLSVRTGQPVMKEILARFPLTFE
                     ITMLTIILGTILGILFGIIAAVYRGSVMDMIVRIFGMIGLATPSFWLGTMIIYFLSVH
                     FGIMLNAGNYVPFSQDPIQNLKQVISPVICMGFSFSASVMRTTRSSLLEEINKEYALT
                     SRAKG"
BASE COUNT         1042 a          647 c          864 g         1042 t
ORIGIN      
        1 gtacatagtg aacttttttg atgggaaaaa atatctgtat tctttttatc agtgagagtg
       61 tatttttgct tttggctgta tcgcttgtcc gtgattgaat aaccacatcc ccaataggat
      121 caatacacct ccgatatatg tagtgctgtc cgggatttca cctgcgataa agaaagccaa
      181 tataccggat aacagcgggt tgaaaaacat ataattactg acctgcgaag ttttttcggc
      241 tttttgaaaa gcggtggacc atgagatata agcaatggtg ctggtaaaga ttcccaatat
      301 caacagataa aaaatctgat ttggaggagc ggttttcaat tcacggaaac tttgcggtgc
      361 ggaaaatgat agcataagtg tcccgatcag gatgctgaaa gcggttacct ggaatccgct
      421 gtattttttt gtcagaattc gttgaattaa gttgtagaca cttaataaaa aagaagctcc
      481 aaatagccag agcgttccga tatttaacga gaaaccgttc cccacgacag tcaatataat
      541 aattccgata aattcgatca gaatcgccag ccattggttt ttagacaagg tttccttaaa
      601 aagaaaaaag gataaaagag ctgtgatcaa cggagaagtt gccatcatga cactggtggt
      661 ggctgattcc acatactgtg tgccaaaatt aaacataacc atatatagag aaaagcctgt
      721 agcggcagaa gcgatcagcc agggaagatc ttttatcaaa ggaaattttg tctttttgat
      781 tatcaacaga agaatgatca aaaaggagga tatcagataa cgaagaaatc cgatcggcat
      841 tgccgagaaa tgccggtggc ataagcgggt taagaccggc gcgactgccc agcataggat
      901 cgttatgatg gcatagaaat gataactgtc tgtgattttt ttgaagttca tcctatttcc
      961 cagagtaagt atcttattaa atgtttggat cgggatcctg aaaacctaaa ttacggaaga
     1021 attatgtcgg ttttgtttcg gtgttttgag tgaatttggc atttcttccg acccggtaag
     1081 ttttcgataa aaaaccgaac tgttcatata aaaagtacaa caaattataa tggaagaata
     1141 ttacggagaa attaacttga ttggataatc tctccccctg caaactgaaa aagaacgcag
     1201 gagaatcgaa cagggtcgag ggcgagccgg tcggcttcat ttgacaaaaa gaaaattttc
     1261 agataaggtt ttatcagtac ggtttcgttc tatattgaaa agaattaaaa ggagaaaaga
     1321 tgaaaaaaaa tattctttat gttgtcctgt ttttgttgat ttttgcggtt ttgccggtta
     1381 atgccgcggg tgatacactg gttattggtg tagccacgga tatgaccggt atggatcctc
     1441 atcaacaaac ggctttcact tcaatgaaag ttcttgaaat gatgtatggc caactggttg
     1501 aagtaaatga aaatatggaa gttgtgccga gtatggcgga atcgtgggag tggagtgagg
     1561 acgggatgca tctgacgatg catctggttc cggagatgac atttcatgac ggttcgccgg
     1621 taacttctgc ggatgtgaaa tattctttcg aacgcattct gaacgaagag accgctgccg
     1681 cagcccgttc aaaatttacg gctatttctt ccatcgatac tccggatgat ctaacggttg
     1741 tttttaactt cggcgagaaa aatgtcgcca ttttagtgga catggcttcg accaatgcgg
     1801 cgattctatc gaaggctttt atggaaaacg gcggcgatct gtcaacgacc gaaatgggat
     1861 ccggtccgta tatgatgaaa aaatgggaac cggataataa aatcgaaatg gttgcctatg
     1921 aaaaatattt tatgaaaggg ttgccgaaga ttaaaaacct ggaattcaga attattcctt
     1981 ccgaagattc gattctggcc ggaattcgta caggtgagat tgatttcgca gtgattaatg
     2041 agcctgctac tgccatgatc gcttccaaag ataaaaatgt tgtcgtgatg gaagctccgg
     2101 ctctttctta ccatgtatta cagttaaatc cgagcagaga agttttcaag aatgtgaaag
     2161 ttcggcaggc aattgcctgt gccattgatc ggcaggaagt cttggatgtt gcctctttgg
     2221 gtgaagggga agtaactccg cccgccgtac agccctttta taaagcgaat ttggatgacc
     2281 tgttttgcta taaaaaagat ttggaaaaat caaaagcttt gttggcggaa gcgggaaacc
     2341 cccaagttga atttacgatt atggctgccg cggatgaacc gccgaccgct gttgcggaag
     2401 cacaaaatat tcaagatcaa ttgtccgaaa tcggcataaa agtaaatatc gaggttcttg
     2461 aattgggcgt ttatgtggat cgctggttag ccggtgattt tgatgcctgc accgctttaa
     2521 acggcggagg atccgatccg aacggaacat tctggcgcta ttggcattcg actgggaacc
     2581 tacaaaaagt ttcgggttat agcagtccgg aacttgataa acttttggaa gaaggacgtt
     2641 cgtacaccga tcccgaagtt cgaaaaggtt attaccttga agttcagaag aatttagccg
     2701 aaaatgcccc ttgggtttgg ttgtatgtcg ggtacgaata tcgggtgatg gttccttcgt
     2761 tgaaaggatt tgtccccact cccaacggtt ctctctggtc gttaagacag gtatattttg
     2821 aataattccg gtgtttttga accggagcgt ttttgcacta aaaatttatc ttccggctca
     2881 ttcagacgaa tgagccggaa ataaggtttc ttccgaataa tggtatgaga aattacattt
     2941 ttagaagatt attggagacg atacctacat tattggggat atctatcgcc atttttttga
     3001 tcatgcatat gattccgggt gatacgatca gtgcgctgat cggaacacaa taccaactga
     3061 ccgaagcaca ggctgaatcg cttcgggcgt attacggatt ggataaatca ttgccggatc
     3121 aatatatcag ctggctgttg aatgcgttgc aaggtaattt cggattatcg gttagaaccg
     3181 gtcagccggt tatgaaagaa attttagccc gattcccatt gactttcgag ataacgatgt
     3241 tgacgatcat tctggggacg attttgggga ttctttttgg gattatcgcg gctgtgtatc
     3301 ggggttccgt gatggatatg attgttcgga ttttcggaat gatcgggttg gctactccga
     3361 gtttttggtt aggaacgatg atcatttatt tcctttcggt tcatttcgga attatgctaa
     3421 atgcgggaaa ttatgttccg ttcagtcagg atccgataca aaatctaaaa caggtgatct
     3481 ctcccgttat ctgtatggga ttttcattca gtgcttccgt aatgcgtaca acgcgttctt
     3541 ccctgctgga agaaatcaat aaggaatatg cactgacttc cagagcgaag ggtaa
//
LOCUS       DAOMIV010000180         3580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_27344_length_3580_cov_4.432057, whole genome shotgun sequence.
ACCESSION   DAOMIV010000180 DAOMIV010000000
VERSION     DAOMIV010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27344_length_3580_cov_4.432057"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..898)
                     /gene="infC"
                     /locus_tag="PKV59_05210"
     CDS             complement(134..898)
                     /gene="infC"
                     /locus_tag="PKV59_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQP46185.1"
                     /translation="MVYCLFGFSVGIYQNSADNKEGVIISTQDFRVNETIRVPEVRVI
                     DDKGNNVGVIPIKEALQLARDADLDLVEVSPNADPPVCRVMDFGKFLYERTKKEKEAR
                     KTQTKIEVKEIRLRPKTNEHHRSFKTRDARRWLEDGMKVRVTVRFRGREITYPELALE
                     DLKEIAEELSDIAAIEQLPAMEGRVMSMVLAPNIKKVVRKKTESSSAAVNTAKNSKNS
                     KKSSLPKEMPEEEIDSDDIEADEEEDDEIVSDTDLE"
     gene            complement(1006..1851)
                     /locus_tag="PKV59_05215"
     CDS             complement(1006..1851)
                     /locus_tag="PKV59_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HQP46186.1"
                     /translation="MEINQTKTVIETINENYDQIFSAEKKVADFILSFPELAVNANVS
                     ELANYSNVSDATVIRFCKHIGYQGYYQLRIILSRDLGRKQVYESEDLSGEDQSVNGLF
                     KMFASNFLKIGQNLDDETILACASVIRNCNQAHVIAVGNTSPLALYMGFRLGRLGIKS
                     SYGMIPEYFMNHINLAEKNDVVIAISQSGCSKLVVQGMELAKEKGLVTIAITAHKYSP
                     VSRLADYLLLSTVGEKSFYYYKSYSHLYEAGVVEALLYYVTNEDKINDKSADKPEIIL
                     SESKL"
     gene            complement(1870..3054)
                     /locus_tag="PKV59_05220"
     CDS             complement(1870..3054)
                     /locus_tag="PKV59_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-dependent glucokinase/phosphofructokinase"
                     /protein_id="HQP46187.1"
                     /translation="MTKDIVLGLGNNIDYEIEWDSAIFENLVHEFGIEYSEIHPVGEV
                     QTQRDLIISILGYMKEGKGGECFVRSSQIIEDFSKAFRKKITLGGTSVRAAIAMSKIG
                     YSSALHLVTMNDDVRRLLPPDCEYICSAPEENSYPHLIIQSTQNHTIRVQDKIIRPKQ
                     ANRIIYDNDLDNILMRLDPRLSQLLLNAKVFLISGFNAMQDQSLLEDRLEKLLISMKN
                     LPKDALVFYEDACFYNKNFSRIVRDKLLGHIQIFSLNEDEFEGYIGRKINLLDPLEVL
                     QSLEVLCKLIPVPKIVLHTQYWALAYGRDADSLKKALKGGITMGGTRYRFGDDFTRQN
                     YFEIEKSAPQKEGAQFSDRINAMKQGEISCLPCVEVNDKNVTTIGLGDAFVGGFLPAL
                     VH"
     gene            complement(3076..>3580)
                     /locus_tag="PKV59_05225"
     CDS             complement(3076..>3580)
                     /locus_tag="PKV59_05225"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HQP46188.1"
                     /translation="EAAHAVNVSVEGELGTIGSTDAQAEQGMNKIIYTNPADAVKFVR
                     ETGIDSLAIAIGTSHGLYPKGMKPELKLDLLKEIKSIISIPLVLHGGSNNRDDEIREA
                     VNLGINKINISSDIKAAYFMKMREVLNDLSLREPNVIQPSCIAAMKEVAYQKIDLFLA
                     TGKASLY"
BASE COUNT          996 a          813 c          609 g         1162 t
ORIGIN      
        1 cttcagcttt aattttcctg ttgtctgctt ccgtggcacg gccgactcct tcatataaaa
       61 tcaaatgagc caataatggc tttcatttac ttagcgggct tattatactc ttattttctg
      121 ttttaggcac agattattcc aaatctgtgt cggaaacaat ttcatcgtcc tcttcttcat
      181 ccgcctcaat atcatccgaa tcgatttcct cttccggcat ctctttcggt aaagaagatt
      241 tttttgaatt cttcgaattc tttgcggtat taacggcagc agaagagctt tccgtctttt
      301 ttcgaacaac ctttttgatg ttcggagcaa gcaccatact cattactctt ccttccatcg
      361 ccggcagttg ctcgatagct gcaatatcac ttaattcctc ggcaatttcc ttcaaatctt
      421 ctaatgccaa ttcaggataa gtaatttcgc gtccacgaaa ccttacagtc acgcgcacct
      481 tcattccgtc ctccaaccaa cgtcttgcat ctctggtctt aaaggaacga tgatgctcat
      541 ttgttttagg ccgcaaacga atctctttca cttctatctt cgtttgggtc tttcgcgctt
      601 ctttttcttt tttagtccgt tcatagagaa attttccgaa atccattacc cgacaaaccg
      661 gcggatcggc gttgggtgat acttccacca aatccaaatc cgcatctctt gccagctgta
      721 acgcttcctt gatcgggatt accccgacat tatttccctt atcgtcaata acacgcactt
      781 ctgggactct gatcgtctcg ttaacacgaa aatcttgtgt actgataatt actccttcct
      841 tattgtcggc tgaattctga tagattccga ctgaaaatcc gaataaacaa tataccacat
      901 ccttaatata tccgcaaacc aaaaatgatt attgccattt ttgaaatgta ttttatgaag
      961 agttctcaat ctttaaaatg atttctcaaa attgaaatcg attttttata attttgattc
     1021 cgaaagaatt atttccggct tatccgcgct tttatcattg attttatctt catttgttac
     1081 ataatataat aatgcctcga ctactccggc ttcatataaa tgagaatatg atttgtaata
     1141 ataaaaagat ttttcaccga cggtagataa gagcaaataa tctgccaatc tgcttacggg
     1201 ggaatattta tgagcggtaa tcgctatggt aaccaatccc ttttctttcg cgagttccat
     1261 accttgaaca accagtttcg aacagccgga ttgagaaata gcaattacaa catcattctt
     1321 ttcggcaaga ttgatatgat tcataaaata ttcggggatc atcccgtatg agctttttat
     1381 ccccagcctt cccaatcgaa atcccatata aagagccaga ggacttgtat ttccgactgc
     1441 gatcacatgc gcctggttac aattcctgat aacgcttgca caggctaaaa tcgtctcgtc
     1501 atcaaggttt tgtccgattt tcagaaaatt agaggcaaac attttgaaca aaccattgac
     1561 ggattgatct tctccgctca ggtcttcact ctcataaacc tgttttcgtc ccaagtctct
     1621 tgagaggata attcgtaact gataatagcc ctgatatccg atatgcttgc agaatcgaat
     1681 gacagtcgca tcgctgacat tgctgtaatt cgccaattct gagacatttg cattaacagc
     1741 cagttcggga aaactcaaaa taaaatccgc cactttcttt tctgcggaaa agatttggtc
     1801 ataattctca ttaattgttt cgataacagt tttagtctgg tttatttcca ttgacccctc
     1861 tgaatagaat taatgaacca gtgcaggtag aaatccaccg acaaaggcat ctccgagtcc
     1921 gattgtagtt acatttttat cattaacttc cacacatggc aaacaagaga tttcaccttg
     1981 tttcattgca ttaatccgat ctgaaaattg agccccttct ttttgaggag cgcttttttc
     2041 tatttcgaaa tagttctgac gggtaaagtc atcgccgaat cgatagcgag tacctcccat
     2101 ggttattccc ccttttaaag cttttttgag agaatcggca tcccttccat aagctaacgc
     2161 ccaatattgg gtatgcaaaa cgatttttgg aacaggaata agtttacata agacttctaa
     2221 agattgtaaa acttcaaggg gatccagcaa atttattttt cgaccgatat aaccctcgaa
     2281 ttcatcttcg tttaaactga atatttgaat gtgccccaac aatttatctc tgacaattcg
     2341 gctgaagttc ttattgtaaa aacaggcatc ttcataaaaa accaacgcat ctttcggcaa
     2401 gtttttcatg ctgattaata atttttccaa ccgatcttcc aataaacttt gatcttgcat
     2461 ggcattaaat cccgatatca ggaaaacttt cgcattcagc aacaactgac tcaagcgcgg
     2521 gtctaagcgc atcagaatat tatcgagatc gttatcataa ataatacggt tggcctgttt
     2581 cggtcgaatg atcttatctt gaacccggat ggtatggttt tgagtagatt gaataatcag
     2641 atgcgggtaa gaattttctt ccggagcgct gcagatatat tcgcaatccg gcggtaacaa
     2701 ccgccggaca tcatcattca tggtgactaa gtggagggct gaactgtatc cgattttact
     2761 cattgcgatt gcagccctca ccgatgtccc ccctaaggta atctttttcc gaaaagcttt
     2821 tgaaaaatct tcgattattt gcgaagatcg gacaaagcac tcccctcctt ttccttcttt
     2881 catataaccc aagattgaaa taattaaatc tctttgagtt tgaacctctc cgaccggatg
     2941 gatttccgaa tattcgattc caaattcatg aaccaaattt tcaaaaatag ccgaatccca
     3001 ttcgatttca taatcaatat tatttcccaa accaagaaca atatcttttg tcatatttac
     3061 gtatgaaatc aggaattaat ataaggatgc tttcccggtg gcaagaaaga gatcgatctt
     3121 ttgataggct acctctttca tcgcagcaat acaggaaggt tgaatcacat tgggttcccg
     3181 taagctgaga tcattcaaaa cttcccgcat tttcataaaa taggctgctt tgatgtcgct
     3241 ggaaatattg attttattaa ttcccagatt gaccgcttcc cgaatttcat cgtcacgatt
     3301 gtttgaaccg ccatgcaaaa ccaacgggat cgaaatgatc gatttaattt ctttcagcaa
     3361 atctaacttt aattcgggct tcattccttt tggataaagg ccgtgagatg ttccgattgc
     3421 gattgcaagc gaatcgattc cggtttcccg aacaaattta accgcatccg cgggattggt
     3481 ataaatgatt ttattcattc cctgttcggc ttgagcatcc gttgatccga ttgttcctaa
     3541 ttccccttct accgaaacat ttacggcatg cgctgcttcg
//
LOCUS       DAOMIV010000181         3554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_27633_length_3554_cov_2.506145, whole genome shotgun sequence.
ACCESSION   DAOMIV010000181 DAOMIV010000000
VERSION     DAOMIV010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27633_length_3554_cov_2.506145"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1128)
                     /locus_tag="PKV59_05230"
     CDS             complement(169..1128)
                     /locus_tag="PKV59_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HQP46189.1"
                     /translation="MRGKEDPRLIARVARLYYEEGVKQNEIAEKLGLSQAAVSRLLAQ
                     GQKDGIIKVVVNIPRGVYTEIEEKLVEKYGLKDAVVADCSLADDGQGLAREIGYCTAS
                     YLENILRPNDIIGVSSWSTYLLAAVNAMTPINRKTGIQVVQILGGLGNAQSQSHSTHL
                     TSHLSEVVNGTPFFLPVPAVVKSAEIYKTIITDKSIQNIFAMFPFVNTALVGIGNMNP
                     SSLLAASGNVLSEEDSRILRKHGAIGDVLLHFYDKMGNEIDESSLTNHVISMSLSQLK
                     KVERAIGVAGGESKFDAILGALRGRLINILITDQFTAKRLLEA"
     gene            1305..2129
                     /locus_tag="PKV59_05235"
     CDS             1305..2129
                     /locus_tag="PKV59_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQP46190.1"
                     /translation="MTIKELKEKSILYRETTLRIIRDAQSGHTGGDLSCIDILNVLYN
                     EILNISPDRVNDPDRDRYVQSKGHAGEALYAVLSENGFMPKEWTKNVGGYLSPATGHP
                     TKNIPGIEQNTGALGHGLPMAVGMAIAGKMDGRAYRVFTLMGDGELAEGTNWEASMAA
                     SHYQLDNLIVIVDRNGLQISGTTEEVMALEPLRAKFESFGFCVREVDGNDVAALCSVL
                     KEVPFEKGRPSLVIAHTIKGKGVSFMENQVQWHHAVPTDEQFRQAILELENERMAL"
     gene            2051..3082
                     /locus_tag="PKV59_05240"
     CDS             2051..3082
                     /locus_tag="PKV59_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQP46191.1"
                     /translation="MASCGADGRTVPAGDFGTGERKDGALMLRPVEKGIANLECMASV
                     LTAHAEKDSSIIVVTSDSRSSGKVSGFARQFPRQLVEVGIAEQDSVGISAGLASCNKK
                     VFTVSPGSFLSARALEQVKNDVAYSQNPVALIGISSGVSYGGLGATHHSIHDLAVLQA
                     IPGLEVIVPADNNETEAALVSYLENPRPVYIRFGKKAMPRFYQAPGEVKIGKVSVLNE
                     GDDLLILAIGEPLWPAYDAAVALESSGVRCSLASVHTLCPIDRDTIIELASHSRGIIT
                     VEEHSVYGGLGSTVAALLMEAGIYRPMKIIGFPREEMVTGSQADLFRHYGIDAENIRK
                     TAMDLLNRN"
     gene            3093..>3554
                     /locus_tag="PKV59_05245"
     CDS             3093..>3554
                     /locus_tag="PKV59_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQP46192.1"
                     /translation="MTTILAIDQSTSATKAMLFNEQAELTEQVSIRHQQYFPKPGWVE
                     HDAEEIYQNLLRAVKELKEKAPQTFREISWLSITNQRETIVVFDQKSGKPLSHAIVWQ
                     DRRAETFCATLRNSGMGEVVHKKTGLIVDTYFPAGKLTWMKNNIPEVAEGLE"
BASE COUNT          970 a          780 c          890 g          914 t
ORIGIN      
        1 aaatataaaa tgttatagct tttaaaataa attccggagc ctatgacaat cccgataatc
       61 atagcgatcg cggtaaacaa cccatacttt cgttgttcca tatgtttcct ttcggacgga
      121 attaaaataa atgaaatata aaaacccgta caattataat ggtgattttt aagcttccag
      181 caagcgtttt gcggtgaatt gatcggtaat taaaatatta atcaacctgc cgcgtaacgc
      241 tcccaagatg gcatcgaatt tggattctcc gcccgctacc ccgatcgctc gttcgacttt
      301 tttaagctgg gataaactca tggaaatcac atgattggtc aggcttgatt catcaatttc
      361 attccccatt ttgtcataga aatgtaacag aacgtcaccg atcgccccat gtttgcgcag
      421 gatgcgtgaa tcttcttccg ataagacatt tccgctggca gccaacaggc tggaagggtt
      481 catgttgccg atcccgacca atgcggtatt tacaaaagga aacatggcga agatattctg
      541 aatggacttg tctgtaatga tggttttata aatttcggcg gatttgacaa ctgccggtac
      601 cggcagaaag aagggtgttc cgttgaccac ttccgataag tggctggtca aatgagtgga
      661 atggctctgt gattgggcgt ttcccaagcc tcccaaaatc tggacgactt gtatgccggt
      721 ttttcggtta atcggggtca tcgcattgac agcggcaagc agataggtgc tccaagaaga
      781 gacaccgata atgtcattgg gacgaaggat gttttccaga taacttgccg tgcaatatcc
      841 gatttctctg gctaaacctt ggccgtcatc cgccaacgag cagtccgcca cgacggcatc
      901 tttcaaaccg tacttttcaa ccaatttttc ttcgatttcc gtataaacgc ctcgcgggat
      961 gttaacgacc actttaatga tgccgtcttt ctgtccctga gccaacagac gggagactgc
     1021 cgcctgcgaa agcccgagtt tttcggcgat ttcattctgt ttgacacctt cttcgtaata
     1081 gagacgcgcc acgcgggcaa ttaacctggg atcttctttt cctctcattg agttctcgtt
     1141 ttcctgtcgg ttttcaatgt gacaaatgtt acaattattt aaaagattga ataaatattc
     1201 aaaaacgatt gattattcag ttttaaaaag gtatgatcaa ggtaaagaat aatcaccgca
     1261 cactgaataa atattcaaaa gcattttatc acgattgggg cattatgact ataaaagaac
     1321 tgaaagaaaa atcaattctt tacagagaaa caacactcag aatcatccgc gatgcgcaat
     1381 cgggacatac cgggggagat ctttcctgta tcgatatcct gaatgtcctc tataacgaga
     1441 ttctcaatat atcacctgac agagtgaacg atccggaccg cgatcggtat gtacaatcca
     1501 aaggacatgc gggagaggct ttatatgccg ttctttccga aaacggcttt atgccgaagg
     1561 agtggacgaa aaatgtcggc ggatatcttt caccggcaac cggacacccg acgaaaaata
     1621 ttccgggaat cgaacagaat acgggtgctt tggggcacgg tctcccgatg gcagtcggta
     1681 tggcgattgc cggaaagatg gacggaaggg cgtatcgcgt ttttacccta atgggagacg
     1741 gtgaattggc ggagggtacc aattgggaag cctccatggc ggcgtctcac tatcaattgg
     1801 ataatttaat cgtcattgtc gatcgaaacg ggcttcaaat ttccgggacg acggaagaag
     1861 ttatggcgct ggaaccgttg agagccaagt ttgaatcatt cggtttctgt gtcagagaag
     1921 tcgacggaaa tgatgtggca gccctttgtt ccgtattaaa agaggttccg tttgaaaaag
     1981 gcagaccgag tttggttatc gctcacacga taaagggaaa aggggtcagt tttatggaaa
     2041 atcaggtcca atggcatcat gcggtgccga cggacgaaca gttccggcag gcgattttgg
     2101 aactggagaa cgaaaggatg gcgctctgat gttaagaccg gttgaaaaag ggattgccaa
     2161 cctcgaatgc atggcatccg ttctgacggc gcatgccgaa aaagactcaa gtatcatcgt
     2221 agttaccagt gactcccgca gttccggaaa ggtaagcggt ttcgcccggc aatttccgcg
     2281 acaattggtt gaagtcggca tcgcagaaca ggattcggtc ggcatatcgg ccggactcgc
     2341 ctcttgcaat aaaaaagtat ttaccgtttc cccgggcagt tttctctctg cgcgagcttt
     2401 ggaacaggtt aaaaatgatg tcgcatattc tcaaaatccg gttgctttga tcggaatcag
     2461 ttccggtgtg agttacggcg gtttgggcgc gactcatcac tcgattcatg atctggctgt
     2521 cttgcaggca atccccggtt tggaggtgat tgttcccgcg gataataacg aaacggaagc
     2581 ggctttggtc tcttacttgg aaaatccccg accggtttat attcggttcg gtaaaaaagc
     2641 gatgccgcgc ttctatcagg cgccgggcga ggtgaaaatc ggaaaggtct ccgttctgaa
     2701 tgaaggggac gacttgttaa tccttgcaat cggtgaaccg ttatggccgg catacgatgc
     2761 ggcggttgcg ctggaaagca gcggcgttcg ctgttcctta gccagcgtcc acacgctttg
     2821 cccgatcgat cgggatacga ttattgaact ggcttcacat tcccgtggaa tcataaccgt
     2881 tgaagaacat tcggtttacg gcggtttggg aagtacggtt gccgcgttgc tgatggaagc
     2941 cggaatttat cgaccgatga aaattatcgg attcccgcgg gaagaaatgg tcaccggcag
     3001 ccaggccgac ttgttccgcc attatggaat cgatgcggag aatatccgca aaaccgcgat
     3061 ggatttatta aacagaaact gagtcatcgg ctatgacaac catattggca atcgatcaaa
     3121 gtacttccgc caccaaagcg atgttgttta atgagcaggc ggaattaacg gaacaggtca
     3181 gtatccggca tcaacaatat ttcccgaaac caggatgggt tgaacatgat gccgaagaaa
     3241 tctatcaaaa tctgctccga gccgtcaaag aattaaagga aaaagcaccg cagacttttc
     3301 gagagatcag ttggttgagc attaccaacc aacgggaaac gattgtggtt tttgatcaga
     3361 agagcggaaa gccgttgagc catgcgattg tttggcagga tcgccgcgct gaaacattct
     3421 gtgcgacttt gcgaaattcc ggaatggggg aagtggtcca taaaaaaacc ggtttaattg
     3481 tggatactta ttttcccgcc ggtaaattaa cctggatgaa gaacaatatc cccgaagtgg
     3541 cggaaggatt ggaa
//
LOCUS       DAOMIV010000182         3533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_27901_length_3533_cov_3.592007, whole genome shotgun sequence.
ACCESSION   DAOMIV010000182 DAOMIV010000000
VERSION     DAOMIV010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27901_length_3533_cov_3.592007"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1487
                     /gene="ppk1"
                     /locus_tag="PKV59_05250"
     CDS             <1..1487
                     /gene="ppk1"
                     /locus_tag="PKV59_05250"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQP46193.1"
                     /translation="IGTISCFRIMRNADLDIDDEDAADLLSEIETQVRQRRWGEVIRL
                     EIQSGYDEEILPILTENFEISDRDLYEIPGPLDVTFLSKLPSFFNDRQDLFYPPFTPQ
                     PSPAFYGKDDYFSVIREKDVILNHPYEDFDTVIELIRRAAEDPNVLAIKQTLYRVGGQ
                     SPIITSLADAAIRGKQVLVLVELRARFDEENNIQWAKKLEQAGCHVIYGLVGLKTHSK
                     ITLIVRNEEDGIRRYVHLATGNYNETTARIYADLGLLTCSEPFGRDATDFFNMISGYS
                     MPLSWRKLIPAPRWLRIDTLNRIEQEMKNAREGKPAAIVAKINSLVDPEIIDALYRAS
                     QAGVRITLIVRGICCLRPGIKNLSENIHVRSIVGRYLEHSRIIYFYNDGQEDLFLSSA
                     DWMPRNLDRRIELQFPVEEKSARMRVFHVLYVQLMDTDRARIMEPDGTYHRVDRRRKE
                     AFDSQVRLCHDAIEAAASLNPEPRMMDRYIPSEVNATAVFKDDK"
     gene            1594..2787
                     /locus_tag="PKV59_05255"
     CDS             1594..2787
                     /locus_tag="PKV59_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQP46194.1"
                     /translation="MKKKETDIKSRDIRIIRIYFLVLIGGMGAANPFLNLYMSRQHFS
                     GAEIGLVISIGSVIGLFAAPFWTRIHMESRYPIKMLSVSLIMSGIATVLFGYQTAFLG
                     MAVFYCLRTLFNAGHFTTADMLALRAIDGTRVGYGSIRLWGSLGWSFVVLLTGWLNGK
                     FSIRSGFFLYAAMNLIAVFVLAQLSPQNRSASAPVNAGVSRYFSGIVDLFRNPALSGF
                     GLMTIITAIGNLGVLNYETIYLDKLGASDSIIGVACMVSAVVEVPMMLISDKMIRRFG
                     PIKVIRTSLFVYSAVRLVVLLFPSVTTVILMRALGGIAFSLLTIGQVVFMNMTLHSEK
                     AGTALVIFTITIPTLINILLTPLFGSIYDNLGAGWLYLISMSGYLLGALIYNRYYQKH
                     PVFKN"
     gene            2942..>3533
                     /locus_tag="PKV59_05260"
     CDS             2942..>3533
                     /locus_tag="PKV59_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQP46195.1"
                     /translation="MRHCQLKILFILSVMILIGWLMSVEIIFAADNENCQSAYLQAGN
                     CVYKTFNNGRLRIRTSPDTSTNVNIIANLDEGTLMTVLSGPICNSGYVFWKVRTANGI
                     VGYVAEGDNYQRWLTPIDPVNCNYLNQKTVPSEIPSDSKGKYCPLRNCACSYLRVGDY
                     VKVSDSGPNAIRSDPDLHPADNIIYRAPSGSGMQIIE"
BASE COUNT          914 a          707 c          867 g         1045 t
ORIGIN      
        1 aaatcggaac gatttcctgc ttccgaatta tgcgaaatgc ggatttggac attgacgatg
       61 aagacgcagc tgatttactg agtgagatcg agacgcaagt tcggcaacga cggtggggtg
      121 aagttattcg tctcgaaatt caaagcggat atgacgaaga gattctgcct attcttacgg
      181 aaaattttga aatcagcgac cgggatctgt atgaaatccc gggaccgttg gacgtgactt
      241 tcttatcgaa gctaccttct tttttcaacg atcgtcagga ccttttttac cctcccttta
      301 ctcctcagcc ctcgccggct ttttacggaa aggatgatta tttttcggtt atccgggaga
      361 aagatgtgat cttgaatcat ccttatgaag attttgatac cgtaatcgaa ttgattcggc
      421 gagcggcaga agatccgaac gtgcttgcga tcaagcaaac cctttatcgg gtgggtggcc
      481 aatcaccgat tatcacttct ttagccgatg cggctatccg cggaaaacag gtcttggtat
      541 tggttgagct gcgggctcgg ttcgatgaag agaataatat tcaatgggcg aagaagttgg
      601 aacaagccgg ttgccatgtg atctacggat tggtcggctt gaagacgcac agcaaaatta
      661 cgttaatcgt acggaatgaa gaagacggga ttcgacgcta tgtgcacctt gctaccggca
      721 actataatga aacaaccgcc agaatatatg cagatttagg gcttctgact tgttccgaac
      781 cgttcggtcg cgatgccacg gattttttca atatgatttc cggttattct atgcccttat
      841 cctggcggaa gttgattccg gcgccgaggt ggctgcggat cgatactctg aatcggattg
      901 aacaggaaat gaaaaatgct cgggaaggaa aaccggctgc catagtggca aagattaatt
      961 cgctggtaga tccggaaatt atcgatgctt tgtatcgcgc ttcgcaagcg ggggtgagaa
     1021 ttaccttaat tgttcgcggt atttgctgcc tacgcccggg cattaagaat ttatcggaaa
     1081 atatccacgt ccggagcatt gtcggacgat atctcgaaca ttcacggatt atttattttt
     1141 ataatgacgg tcaggaagac ctgtttcttt cgagcgctga ttggatgccg cgaaatttag
     1201 atcgccgaat cgaattacaa ttcccggtcg aagaaaaaag tgcacggatg cgggtatttc
     1261 atgttttata tgttcagctg atggatacag atcgagccag gataatggag ccggacggga
     1321 cgtatcatcg ggtggatcgt cgtcgtaaag aagcgttcga cagtcaggtt cgattatgcc
     1381 atgatgccat cgaagctgcg gcttcattga atcccgagcc gaggatgatg gatcgttata
     1441 ttcccagtga ggtaaacgcg accgcggttt tcaaagacga caaataaatt atagaaaaca
     1501 acgatgattt attgacacga tttccttgag atgagatcat tttttattcg gattccctta
     1561 aaaataatgg attatctgat acattaaagc aatatgaaga agaaagaaac ggatattaaa
     1621 agccgtgata tcaggattat ccgcatctat ttcttggtct tgatcggcgg aatgggtgca
     1681 gccaacccgt ttcttaatct atatatgtcc cgtcaacatt tttcaggtgc tgagatcgga
     1741 ttggttattt ctatcggttc ggtcatcggg ttgtttgccg ctccattttg gacgagaatt
     1801 catatggaga gccggtaccc gattaaaatg ctttccgttt cactgattat gagcggaatc
     1861 gcgacggtac tattcggtta tcagacagca tttttgggga tggctgtttt ttattgtctg
     1921 cgtacgttgt ttaatgccgg acattttacc accgcagaca tgctggcttt acgggcgatc
     1981 gacggcactc gagtcggcta cgggagcatt cgtttatggg gttcattggg atggtctttc
     2041 gtggtgctgc ttaccggttg gctgaacggc aaattctcga ttcgatccgg cttttttctg
     2101 tatgcggcaa tgaatttgat cgccgtattt gtactggcac aattaagtcc acagaatcgt
     2161 tctgcatcag ccccagtaaa tgccggtgtt tcgcgatatt tcagcggaat tgttgattta
     2221 ttccgtaatc cggcactatc cggtttcggc ttgatgacaa ttatcaccgc aatcggtaat
     2281 ttgggtgtct tgaattatga aacgatttat ttggataaac tgggtgccag tgacagcatt
     2341 atcggtgtcg cctgtatggt gagtgcggtg gttgaggtgc cgatgatgct catcagcgat
     2401 aaaatgatcc gtcgattcgg accgataaaa gtaatccgta cttcattatt tgtttacagt
     2461 gcggtgcggc tggtggtgtt gttattcccc agcgttacaa ccgttatttt aatgcgtgcg
     2521 ttgggtggaa tcgctttcag ccttttaacg atcgggcaag ttgtatttat gaatatgact
     2581 ttacattcgg agaaggctgg aacggcattg gtgattttta caattaccat tccgacactc
     2641 atcaatattc ttttgacacc tttattcggc tcgatctacg ataatttggg agccggttgg
     2701 ttatatctta tcagtatgtc agggtatttg ctcggtgcgc ttatttacaa tcgttattat
     2761 caaaaacatc cggtatttaa aaattaatat ttgttgaaat aaccgaatat taaatatttc
     2821 ggcagacact cgatgataaa cttgcctatt aaagtcagtt tacattgcga agttgcaacc
     2881 attccgccgg taaaaacgtc tttgaaatga agaataaatc gatcggagag cggaggttcc
     2941 tatgcgacat tgtcaattga aaatcttgtt tatcttatcc gttatgatat taatcggatg
     3001 gttgatgtcg gtggaaataa tttttgcggc ggacaacgaa aattgtcaaa gcgcatattt
     3061 gcaagcggga aattgcgttt ataaaacctt caataacggt cgcctccgca tccgaacttc
     3121 accggatact tctacaaatg taaatattat tgccaatttg gatgaaggaa cattaatgac
     3181 tgttctttcc ggtccaatct gtaattcggg gtatgttttt tggaaagtga gaacggcgaa
     3241 tgggattgtc ggttacgtgg cggaaggaga caactatcaa cgttggctca cgccgatcga
     3301 tccggtgaat tgtaattacc tgaatcaaaa aaccgttccc tcggaaattc cctccgattc
     3361 gaaaggaaaa tattgtccgt tgaggaattg cgcctgttct tatctcaggg tcggcgacta
     3421 tgtcaaagtt tcggacagcg gccccaatgc gatccgaagc gatcccgatt tgcatccagc
     3481 cgataacatt atttatcgcg ctccttccgg aagcggcatg caaattatcg aag
//
LOCUS       DAOMIV010000183         3523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28004_length_3523_cov_4.084487, whole genome shotgun sequence.
ACCESSION   DAOMIV010000183 DAOMIV010000000
VERSION     DAOMIV010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28004_length_3523_cov_4.084487"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..772
                     /locus_tag="PKV59_05265"
     CDS             218..772
                     /locus_tag="PKV59_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46196.1"
                     /translation="MISEWNKSIWLTRSAFMPGQNWLVNGAILKGEKMALVWETLAYP
                     ADMQAMSFLLAKFDFVVAAYSHADWDHCLGTNGFSFSNVIAQQNAIRRFKFELPATLK
                     KLNAKYSKAWEDVCPVPPDFVFSDKLNLDFGSFRLELSSFPDIHPMGWWAISPNIRCS
                     WPVTVLKKFLSLMIRKKFLPGLNT"
     gene            977..1870
                     /locus_tag="PKV59_05270"
     CDS             977..1870
                     /locus_tag="PKV59_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQP46197.1"
                     /translation="MNSAEKSALQKKSNLILLLTAIIWGSGFIPQKICANSMTAFAFN
                     GFRYLIGSLSVLIITHFRLPENRRAWRITFLSGTILFLAADLQQFGIRYTTVGNTGFI
                     TTIYVVLVPFMGWLLFRQKITRSAFWAAAIALIGMYLLSTSGKALSTISAGDGIVFIG
                     SVFWAIHILVVGKAMGRVDAMRFSAGQFLVCAFWNLLCWLIFDRGNLSGVRESIPLLL
                     YTGIVVIGGGFTLQAVGQKHADESQAAIILGLEAVFASFFGILFFRESFSFIQAAGAA
                     LIFISVVISVKGTPNLELPPI"
     gene            1999..3462
                     /locus_tag="PKV59_05275"
     CDS             1999..3462
                     /locus_tag="PKV59_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46198.1"
                     /translation="MEQNKKITWFEGIVNPEIEEANPLLSWIWCGFLFLLGMFLWGYF
                     LNFGKIPFDFHDWAEVNAPRIAFLKDAVTKGVLPLHMQDASALRGVTDRYMALPDVLL
                     SPQILLLKWLDVGDFILIHTWLLYAIGFWGLYRLKKSMHLSLFGFTWFFLLFYFNGHI
                     TSHYAVGHITWGGYFLFPWFVYLVIRMINGDHSWKWVAKISFLLFFIFLQGSFHQFIW
                     CLLFLAFLALTGRKNLFTVLKAGIFSCLLSMVRIIPPALQASAFDDDFLGGFTSPIQL
                     IRSFIRIIPPSESLNSAVTGGNLGWWEFDHYIGVSGALFLLIFGILWLKNRRKETGYP
                     AILVPMIVLTFFSIGKFYGIFRLLPIPLFSGERVSARFLILPLVFLMAEAVYAMQQSM
                     EIRKFDVLSVSIPLLILLFGLPEFWSHLETWKVLNAFQAFPYTYTDLTIKTVANHPDP
                     SYTNGLLIGMAGTILTAILLAGLVWREDRHIKNMLVP"
BASE COUNT          844 a          698 c          846 g         1135 t
ORIGIN      
        1 gcggataaag cataggtcaa atccagaatg gtccaagcat cctcttcaaa acaaactttt
       61 tccagataga ggacagattc caaatcccgt atggaagcat gcagaatcac gatagccata
      121 agctgaattt tatcctaaga atgatccttg ttaagaaccg tttcagacaa ataggagagt
      181 tctttgacga cttttccatt ataatcagcg aatgccgatg atttcagaat ggaataaatc
      241 catatggttg acacgcagcg ctttcatgcc cggtcaaaac tggttggtta acggagcaat
      301 tctgaagggg gagaagatgg ctcttgtatg ggaaacgctg gcttatccgg cggatatgca
      361 ggcgatgagt ttccttttag ccaaatttga ttttgtcgtc gccgcttatt ctcatgcgga
      421 ttgggatcac tgcttgggga caaacggatt ttctttttcc aatgttatcg cacagcagaa
      481 tgcaatccgt cgctttaaat tcgaactacc tgcgacgctt aaaaaactta atgcaaaata
      541 ctccaaagca tgggaagacg tctgccctgt tcctcccgat tttgtttttt cggacaaatt
      601 aaatctggat tttggctcct tccgcctgga attgtcctcc ttcccggaca tacacccgat
      661 gggatggtgg gctatatccc cgaatataag atgctcctgg ccggtgacag tgttgaagaa
      721 attcctgtca ttaatgatcc gaaagaagtt ccttcctgga ttaaacacct gaagaaatgg
      781 ttaaccgagc cggatattca gtcggttttg ccggggcacg gcgaaattac cgacttatca
      841 ctgcttgaga agaatctgaa atacctgatt gctttgacgg aaaaatcgcc cttggatgtt
      901 gagacggaca acccgtttta tctggatgtt catcaggaaa attgcaaatt gatggggtgc
      961 tctgttggaa aatgaaatga attccgcgga gaaatccgcg ctgcaaaaaa aatccaatct
     1021 gatccttttg ctgacggcga ttatttgggg cagcggtttt attccacaaa aaatctgtgc
     1081 gaattcaatg accgcttttg cattcaacgg gtttcgttat ctgataggtt ctttatcggt
     1141 tcttatcatt actcattttc gccttcctga aaatcggagg gcttggcgca ttacattctt
     1201 atccggtacc attcttttcc tggcagccga tttacaacaa tttggtatcc gatatacgac
     1261 ggtgggaaat accgggttta ttacaacaat ttatgtggtg ttggtgcctt ttatgggatg
     1321 gctgcttttt cggcaaaaga taacccgaag tgcattttgg gcggcggcga ttgcgttgat
     1381 cggaatgtat cttctatcca cttcggggaa agctctcagt accatatccg ccggagatgg
     1441 aattgtcttt atcggctctg ttttttgggc aattcatatt ttagtggtcg gcaaggcgat
     1501 gggaagagtc gatgccatgc gattttcggc aggtcaattt ttggtatgcg ccttttggaa
     1561 tcttctgtgc tggctgattt ttgatcgagg gaatctcagc ggtgtcagag aatcgattcc
     1621 tttgctctta tacacgggaa tcgttgtgat cggcggcggc tttacattgc aagcggtggg
     1681 tcaaaagcat gcggatgaat ctcaagccgc aatcattttg gggctggaag ccgtttttgc
     1741 ctcttttttc ggaattttgt ttttcagaga atctttttcg tttattcaag cggccggtgc
     1801 ggctttgatc ttcataagcg tcgtcatttc ggttaaggga acgccgaatt tagaattgcc
     1861 tccgatatag tttttttgat gaagtttctt ttttgaatga acggacggaa ttgaaaagcg
     1921 atcaggaata ttttctttgt gcagccggtg aagaaaaaag tttgcttaac tgattcaaca
     1981 aatttacaat cattaaatat ggaacaaaat aaaaaaatca cttggttcga aggaattgtt
     2041 aatccggaaa ttgaggaagc aaatccgtta ttaagttgga tttggtgcgg atttctgttt
     2101 ctgttgggga tgtttctatg gggatatttt cttaatttcg gaaaaattcc ttttgatttt
     2161 cacgattggg ctgaggtaaa tgctcccaga atcgcatttc tgaaagatgc cgtgacgaag
     2221 ggtgtgttac cgctgcatat gcaggacgcg agcgctttgc gcggcgtgac ggatcgttat
     2281 atggcgcttc cggatgtatt gctcagcccg cagatcctat tgctgaagtg gttggatgtc
     2341 ggtgatttta ttctgataca tacttggctg ctgtatgcca tcggtttttg ggggctgtac
     2401 cgcttgaaaa aaagcatgca tctttccctt ttcggtttta cctggttttt ccttttgttt
     2461 tatttcaacg ggcatattac ttcgcattat gccgtgggac atatcacgtg gggcgggtat
     2521 tttctcttcc cgtggtttgt atatttggtt attcggatga tcaacggtga tcacagttgg
     2581 aaatgggtgg cgaagatttc atttctgttg ttttttatat ttctgcaagg gtcttttcat
     2641 caatttatct ggtgtctgct ttttttagct tttttggcgc ttaccggccg aaagaatctg
     2701 ttcacggttt tgaaagcggg aatattttct tgtttgcttt ccatggtaag gattattccg
     2761 ccagcactgc aggcatcggc tttcgatgat gattttttag gcggctttac ctctccgata
     2821 caactcattc ggtcgtttat tcggatcatt cccccatccg aatccttaaa ttcggcggtt
     2881 accggcggta atctggggtg gtgggaattt gaccactata tcggtgtatc cggagccctt
     2941 tttctcttga tattcggcat attgtggctg aaaaacagac ggaaagaaac cggctacccg
     3001 gcgatcttgg ttccgatgat tgttctgact tttttttcaa tcgggaagtt ttacggaata
     3061 ttcaggctgc tgccgattcc gctgttttcc ggtgaacgtg taagcgcgcg ttttctgata
     3121 ttgccgctgg tctttctgat ggcggaagct gtctatgcca tgcaacaatc gatggaaata
     3181 cgaaaatttg atgttttatc ggtatcgatt cctttgctga ttcttctatt cgggcttccg
     3241 gaattttgga gccacctgga aacatggaaa gtactgaacg cttttcaggc ttttccgtat
     3301 acctataccg atttgaccat aaaaacagtg gcaaatcatc ccgatccttc ttataccaat
     3361 ggtttactga tcgggatggc tggtacgatt cttaccgcaa tcctgttggc aggacttgtt
     3421 tggcgggagg accgacacat aaaaaatatg cttgtgccat aattggcata gagttgtgaa
     3481 ttatgatttg attcagccaa gctggatcaa atctgtataa gga
//
LOCUS       DAOMIV010000184         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28017_length_3522_cov_3.628209, whole genome shotgun sequence.
ACCESSION   DAOMIV010000184 DAOMIV010000000
VERSION     DAOMIV010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28017_length_3522_cov_3.628209"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1249
                     /locus_tag="PKV59_05280"
     CDS             362..1249
                     /locus_tag="PKV59_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46199.1"
                     /translation="MDISRLRKQIAERDSYSFLETVDVFSGKRTQLKEFDFVIEAPNW
                     TKDGRFLVYNSMGKIFRFELSTGTIEEIDTGFATDCNNDHVLSPDNAQLGISHFTNED
                     AVSRIYILPFTGGNPRLVTEKGPSYLHGWSPDGKRLAYCAERNGQFDIYTISVAGGEE
                     TQLTDQPALDDGPEYSPDGRSIWFNSARTGLMQLWRMNTDGSDQTQMLHEEANCWFPH
                     ISADGKWVVYIAYRKGDVAPGDHPANKDVELRLIPSEGGESKTIARLFGGQGTINVNS
                     WAPDHHTVAFVSYKLKGSD"
     gene            1275..2414
                     /locus_tag="PKV59_05285"
     CDS             1275..2414
                     /locus_tag="PKV59_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP46200.1"
                     /translation="MAIQPVKTAIVGCGVISEAYLETMTRKFKILNVVGCCDLNQRKA
                     EIRAQKYGIRVLSMEEILADPSIELVVNLTTPTAHYGVIKQLLEGGKHVYTEKVLSVE
                     LEQAAELVKIADQKGLYLGAAPDTFLGSAIQTARYVVDSGMIGEISSCACTLTRDSEL
                     FSRAFPFTAQPGGGIGFDVGIYPITALLSILGPVAEVSGIIQTRKPERYQLSPEHFGE
                     PFRIECENLMSGMLRFHCGTIGTLLFDANSIFTQPEKPNLILFGTLGILYMADPNQFG
                     GDVKVILKGNNEPFVMQQSHAFSGECRGLGVAEMAWSLRRGRKNRANKEMAFHALEVL
                     HGIVRSSETRMSIVMQSTFEKMPPIPRGYSGQNFFDFIEEAGIAL"
     gene            2554..3336
                     /locus_tag="PKV59_05290"
     CDS             2554..3336
                     /locus_tag="PKV59_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HQP46201.1"
                     /translation="MALLQINFYSETLHRKVPVQVILPTDVSPGNTEPYKTLYLLHGL
                     TENCMAWTSNTRIERWATERNLAVVMPSGENSFYVDHPFGSSCFGDFGAYIGQELVEM
                     TRKMFRLSTDRADTFIAGLSMGGFGALRNGLKYHETFGYIACLSGAIHIFEYPFDDPD
                     RNIIGEDACFGDIRAAALTDKNPRVAAEILFEKKRTDPTVSFPKVYIACGTEDYLLKA
                     NRQFAQFLRINGVSVTYEEGPGKHDWDFWESYIQRVLEWLPI"
BASE COUNT          982 a          737 c          885 g          918 t
ORIGIN      
        1 agctgaagtt gtctccgtaa ccgatatatt tcgtatagct attttctaat aacagctttt
       61 cactttagaa tttcggattg atatccgaat caaacaaaag acagaggaaa tattttcctt
      121 ttcccgattc caaaaatatc ggaaagttgg ggttacaatt tattaataga taaaaaatca
      181 gtatattcgt ttcactggtt gaatcgcttt ttattcggaa cacggcataa ataaaataaa
      241 accgtatgta ttttgaacgg gttttttcaa tgattcagca accaacagcc ccggcgtttc
      301 tatctccatt ccaatgaaga taatttcgca caactattat gaaaatcgga gtacaaacaa
      361 tatggatatc agtcgtttac gcaaacaaat cgcagagcgg gattcctaca gctttttaga
      421 gaccgttgat gtctttagcg gaaaaagaac ccaactgaaa gaatttgatt ttgttatcga
      481 ggcaccgaat tggacaaaag acggtcgatt tttggtctat aacagtatgg gcaaaatctt
      541 tcgatttgag ttatcaaccg gtacgatcga agagattgac accggttttg caaccgattg
      601 caacaacgac catgttttat ctcccgataa tgctcaactg ggaatcagcc attttacaaa
      661 cgaagatgcc gtttctcgaa tatatatttt gccattcaca ggcgggaatc cgcgtttggt
      721 aaccgaaaaa ggtcccagct atttgcatgg ctggtcaccg gacggcaaac ggcttgccta
      781 ttgcgccgaa cgaaacggac aatttgatat atacacgatt tctgtcgcgg gcggagagga
      841 gacgcaattg accgaccaac ccgccttgga tgacggtccg gaatattctc cggacgggag
      901 atcgatttgg tttaactcag cacgtacagg actgatgcag ctctggcgga tgaatacgga
      961 cggatccgat caaactcaaa tgttgcatga agaagcaaat tgttggtttc cgcatatttc
     1021 ggcggatggg aaatgggtag tttatatcgc ttatcgaaaa ggcgatgttg cgcccggcga
     1081 tcatccggca aacaaagacg tggaattacg actgatcccg tccgaagggg gcgaatcgaa
     1141 aacgatcgcc cgtttgttcg gcggacaagg gacgatcaat gtgaactctt gggcaccgga
     1201 tcatcatacc gtagcttttg tttcatacaa acttaaggga agcgattaat cttctgagga
     1261 aagaggcaaa tagaatggca attcaaccgg taaagaccgc tatcgtcgga tgcggggtga
     1321 tcagcgaagc ttatttggaa accatgacgc gaaagtttaa gattctcaat gtcgtcggat
     1381 gctgtgattt gaaccaacgg aaggctgaga taagagcaca aaaatacggc atccgggtac
     1441 tcagcatgga agaaatcctg gctgacccaa gcattgaact tgttgtcaac ctgaccacgc
     1501 ccacagcgca ttacggtgtc attaaacagc tgttggaagg tggcaaacat gtttatacgg
     1561 agaaggtttt gtctgtggaa ttggaacaag ccgccgagtt ggtcaaaata gccgatcaaa
     1621 aagggttata tctgggagcg gctcccgaca cctttctggg ttcagcgatc caaacagcac
     1681 ggtatgttgt ggacagcggc atgatcggcg aaatttcttc ctgtgcatgc accttgacgc
     1741 gtgacagtga actgttcagc cgcgcctttc ctttcactgc tcaaccgggc ggaggaatcg
     1801 gttttgatgt gggcatctat cccatcactg ccttattgag cattttgggt ccggttgccg
     1861 aggtgtccgg aattattcag actcgcaagc cggagcgata tcagctttca ccggaacatt
     1921 tcggtgagcc tttccgcatt gaatgtgaaa atctaatgag cggtatgctg cgatttcatt
     1981 gcggaacgat cgggacactt ttgttcgatg cgaattcgat ttttactcaa ccggagaaac
     2041 ccaatttgat tttgttcgga acgctgggaa ttctgtatat ggcagatccc aatcagtttg
     2101 gcggtgatgt caaggtgatc ttaaaaggaa ataacgaacc tttcgtgatg caacaaagtc
     2161 atgctttttc cggcgaatgc cgcggactcg gcgtagccga aatggcttgg tcattgcgac
     2221 ggggaagaaa aaatcgggcc aacaaagaaa tggcatttca tgccctggaa gtattgcacg
     2281 gtattgttcg cagtagtgag acccgaatga gcattgtgat gcaatccacc ttcgaaaaga
     2341 tgccgcctat cccgcgaggc tattcgggtc aaaacttttt tgattttatc gaagaggcgg
     2401 gaatagcgct ttagcgatca tccggtcgtg ctgcagggat tttcaaagag ccggttgcat
     2461 ataaggggaa gcgtgtccta tagaaatgat cggcaaatca cccattgatg ggacggccga
     2521 tcgatcagac cgaaagaaag gagaaaagac gcgatggctt tgcttcaaat aaatttttac
     2581 tccgagacac tgcatcgaaa agttccggtg caggtaatcc ttcccacaga cgtgtcaccg
     2641 gggaatacag aaccgtataa gacattatat ttacttcatg ggctgacgga aaactgtatg
     2701 gcttggacat ccaatactcg gattgagcgt tgggcaaccg agcggaattt ggccgtagtg
     2761 atgccgtccg gggaaaattc gttttatgtg gatcatccgt tcggcagttc ctgtttcggg
     2821 gatttcggtg cttatatcgg gcaggaattg gtcgaaatga cacggaaaat gtttcgtctt
     2881 tcaaccgatc gggcagatac gtttattgcc gggctttcga tgggcggatt cggtgcgctg
     2941 cgaaacggtt tgaaatacca tgaaactttc ggatatatcg cttgcctctc cggcgccatt
     3001 catatttttg aatatccgtt tgatgatccc gaccgtaata tcatcggaga agatgcctgt
     3061 ttcggtgaca ttcgagccgc ggcgctgacc gataaaaatc cgagagtggc tgcggaaatc
     3121 ttattcgaaa agaagcgaac ggatccgacc gtttctttcc cgaaagtata tatcgcctgc
     3181 ggtacagagg attatttatt gaaggcaaat cggcaattcg ctcaattttt acggataaac
     3241 ggagtctctg tgacttatga ggaaggaccg ggaaagcacg actgggattt ttgggaatcc
     3301 tatatccaac gggtgcttga atggcttccg atctaaaatc attcaaaaga tgaaaggaaa
     3361 tagggaatta aaatggaaaa acaaactgca ggaagaaccg ctctcggtac ctttgcacca
     3421 aaatttgccg aactgaacga tgatgtgctg ttcggagaag tatggtcaag agaagaccag
     3481 ctttcgttgc gcgatcgaag cattattacg gttacggttt tg
//
LOCUS       DAOMIV010000185         3513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28120_length_3513_cov_4.064488, whole genome shotgun sequence.
ACCESSION   DAOMIV010000185 DAOMIV010000000
VERSION     DAOMIV010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28120_length_3513_cov_4.064488"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="PKV59_05295"
     CDS             <1..1485
                     /locus_tag="PKV59_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46202.1"
                     /translation="TPLSFYQATITYTIKSGILWSDGETLSAQDSVYSWRLAGDPAIP
                     GSKKMFNLTKSYKATDDQTVVWQGVPGFIPQKASDVFWLPLPEHVMGNMKAADILVDE
                     GINRTPIGWGAYKIAEWIPGDQITVVRNAAYRNTETGVAPFFDKVIFHFYGKAGDNNL
                     AALKSGSCDIVDTSVDLLPNLEPILEDVRDGKISAYIQPQTVWEQLTLNFAPLNQKGF
                     GYFADVGVRKGIARCLDREALIRQVLYGQSEIPQGFYPSQHSLFAGNLTDTEFDPVAG
                     RALLEDAGWIDSDGDSTTPRVSSGVEGVPDGTEFAVTLETSESAERIKTAELIRDQLA
                     DCGISVTVQAEDQAKIYAQGPEGSVFGRKFDMAMFAWASGDQSPCSLYSSSQIPTEEN
                     QWIGLNVGGYRSAEYDSACNIAQNASPMEEDYLGKAEKVQQLFAEDLPVIPLYFYPAI
                     GLSGNRICGLKNSIGMRSLLYNIESLTRSDSNCAVSQWKNIYSE"
     gene            1550..1774
                     /locus_tag="PKV59_05300"
     CDS             1550..1774
                     /locus_tag="PKV59_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQP46203.1"
                     /translation="MEHKKLYRSQKDRFIAGVCGGLAKYFGIDATIIRVAWIVLIFTT
                     GFIPFVLIYILCALIIPLEPTSPKDVTIDQ"
     gene            complement(1856..3145)
                     /locus_tag="PKV59_05305"
     CDS             complement(1856..3145)
                     /locus_tag="PKV59_05305"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HQP46204.1"
                     /translation="MNNEEINPFELAQKQFDHVADMLELPEDVRSYLRWPRKEFHVRV
                     PLRMDNGELKLFNGYRIQHNNALGPNKGGLRFYAGETVDTVRALATWMTWKCAVANIP
                     LGGGKGGINVDPSTLSEAEKERLIRGYVRLLWKNFGPRVDIPAPDMGTNAQMMCWFMD
                     EYSQQMGQYVPGVVTGKPVGAGGSLGRNSATGYGVVVCIREAMKRLGYNPKGMTASIQ
                     GFGNVGQYSATNFVEMLGGIVKCVSCWDRNDKKSYTYYKKEGVDPRFLMSITDQYGMI
                     NKGKAQEAGYEILDGDAWLSMDVDVLIPAALEGQIRADNVEKISPKLKILAEAANGPT
                     MTEADEVIKNRGYFLIPDFLCNSGGVTCSYFEGVQNDANFYWSEEEVENKLDDKMTIA
                     FNSVYQMAEDRKVYMRDAAYMVAIQRVVTAMRMRGWV"
BASE COUNT          957 a          763 c          796 g          997 t
ORIGIN      
        1 acgccactga gcttctatca agcgacgatc acatatacaa taaaatccgg tattttatgg
       61 tcggacggtg aaacattatc agcacaggat tcggtttatt catggagact ggccggtgat
      121 ccggcaattc ccggttcaaa aaaaatgttc aacctgacga aaagctataa ggcgactgat
      181 gatcaaacgg ttgtctggca gggagttccg ggttttattc cgcaaaaggc ttcagatgtg
      241 ttctggttgc cgttgcccga acacgtgatg ggaaatatga aagcggcgga tatcctggtt
      301 gacgaaggaa tcaaccggac accgatcggt tggggtgctt acaaaatagc ggaatggatc
      361 ccgggggatc agataaccgt tgtccgaaat gcggcatatc ggaataccga gaccggtgtg
      421 gctccttttt ttgataaggt gatatttcat ttttatggaa aagccggtga caataatctg
      481 gcggcattaa aaagcggcag ctgtgatatt gttgatacgt ctgttgatct tctgccgaat
      541 ttagaaccga tcctggaaga tgttcgggac ggaaaaattt ccgcatatat ccaacctcaa
      601 acagtgtggg aacaactcac actgaacttt gctcctctta atcaaaaagg gttcggttat
      661 tttgccgatg taggcgttcg aaaagggatc gcccgctgtt tggatcgcga agcactgatt
      721 cgacaggttt tatatggtca gagtgaaatt cctcaggggt tttatccttc gcaacacagc
      781 ctgtttgccg gaaatctgac tgataccgaa ttcgatccgg tagcaggtcg ggctttgctg
      841 gaagatgccg gatggatcga ttcggacggg gattcgacta caccgcgtgt ctcttcgggt
      901 gtggagggag ttccggacgg aacagaattt gccgtcactt tagaaacctc cgaatctgcc
      961 gaaagaatca aaacggctga attaattcgt gatcaattgg cggattgcgg aatttcggta
     1021 accgttcaag cggaggatca ggcgaaaatc tatgcgcagg gaccggaagg atctgtattt
     1081 ggccgaaagt ttgacatggc aatgtttgct tgggcaagcg gcgatcaatc tccctgttcc
     1141 ctttactcga gcagccaaat tccgaccgag gagaatcaat ggatcggttt aaatgtcggc
     1201 ggttaccgat ctgccgaata tgattcagct tgtaatatcg ctcagaatgc ttctccgatg
     1261 gaagaagatt atttaggtaa agccgaaaaa gttcaacaat tatttgcaga ggatttgccg
     1321 gtaattcctc tctattttta tccggctatc ggattgtccg gtaatcggat ctgcggattg
     1381 aaaaacagta tcggaatgcg cagtcttctt tataacattg aatcgcttac acggagcgat
     1441 tcaaactgtg cggtctctca atggaaaaat atctattccg aatgataatc ggaatatttc
     1501 gaaacaatct ggcgataaat acgtatatca gttagacagg agaaaaaaca tggaacataa
     1561 aaaattatat cgaagtcaga aagatcgatt tatcgcaggt gtatgtggcg gtttggccaa
     1621 gtatttcgga attgatgcga caattattcg ggtggcgtgg atcgtattaa tattcacgac
     1681 aggttttatt ccctttgttt tgatttatat cctttgcgca ttaattattc cgttggaacc
     1741 aacctcgcca aaggatgtca caatcgatca gtaattcata aaaagcggat tggttataaa
     1801 aaagcctatt gccgaatggc aacaggcttt ttttcatcaa gcaattatga gggctctata
     1861 cccatccgcg cattctcatg gcggtaacga cccgttgaat cgcaaccatg taagccgcgt
     1921 cgcgcatata tactttgcgg tcttcggcca tttgataaac ggaattaaag gcaatcgtca
     1981 ttttgtcatc caacttattc tcgacttcct cttcgctcca atagaagttg gcatcatttt
     2041 gaacaccttc aaaataggag caggtcacac cgcccgaatt acacagaaaa tcgggaatca
     2101 ggaaataacc gcggtttttg atcacttcgt ctgcttcggt catggtgggt ccgttggccg
     2161 cttcggcaag gatttttagc ttcggactaa tcttttcgac attatccgct cggatttggc
     2221 cttcgagagc agccggaatc agtacatcta catccatact gagccaggca tctccgtcca
     2281 ggatctcata tccggcttct tgggccttcc ctttattgat cattccgtat tgatcggtga
     2341 tactcatcaa aaatcgcgga tccacaccct ctttcttgta ataggtgtag cttttcttat
     2401 cattgcgatc ccagcaggaa acgcatttga cgatgccgcc caacatttct acaaagtttg
     2461 tggcactata ttgaccgaca ttgccgaaac cttgaatgga tgcggtcatt cctttgggat
     2521 tgtagccgag tcttttcatt gcttcccgaa tgcagactac aacgccatat cccgtagccg
     2581 agtttcttcc gagtgacccg ccggcgccga ccggtttgcc ggtgactacg ccgggaacat
     2641 actgtcccat ttgttgagaa tattcatcca tgaaccagca catcatttgt gcgttggtac
     2701 ccatatccgg tgcgggaata tccactctcg gtccgaaatt cttccagagc aaccggacat
     2761 aaccgcgaat caggcgttct ttttccgctt cactgagcgt ggaaggatcc acattgattc
     2821 cacctttccc gccgccgagc gggatattgg caacggcgca tttccaagtc atccaagttg
     2881 cgagagcgcg gacggtatcg accgtctctc ccgcataaaa tcgtaaaccg cctttattag
     2941 gacctaacgc attgttgtgt tgaatacgat agccattaaa cagcttcagt tcgccgttat
     3001 ccattcgaag cggaacacgg acatgaaatt ctttccgtgg ccaacgtaaa tacgagcgaa
     3061 cgtcttccgg caattcaagc atatccgcga catggtcgaa ttgtttctga gctaattcaa
     3121 acgggttgat ttcttcattg ttcatcctta acgatctcct tttacagcat taaataatta
     3181 tgatttgatt tgtttgatat aagtttatat tcgttttctg ataagagtaa ttgatagatt
     3241 gccgataaat gtcagacatt tatcacttgt cagtatacca tatctgccga actctttttg
     3301 cttgatctgt cacaaataaa attatagaat agataaaatt tttccataag gaaaataaat
     3361 agtaataaat tatctgaaaa aatcgaataa ataattgtcc gtatgactta ttctccatat
     3421 ggttttgtaa aattaaggtg cttttgtgat aaaaggaaat tgtgaaaata aaaacaaata
     3481 ctttccataa agcaaagatt ctgccggaag cgt
//
LOCUS       DAOMIV010000186         3509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28174_length_3509_cov_3.737116, whole genome shotgun sequence.
ACCESSION   DAOMIV010000186 DAOMIV010000000
VERSION     DAOMIV010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28174_length_3509_cov_3.737116"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /gene="glpK"
                     /locus_tag="PKV59_05310"
     CDS             complement(<1..1220)
                     /gene="glpK"
                     /locus_tag="PKV59_05310"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQP46205.1"
                     /translation="MTKYIAAIDQGTTSSRCILFNQAGEIVSSDQMEHDQILPKPGWV
                     EHNPIQILRNVETIFENAVRKADAEPGDIAAIGITNQRETTVVWNRYTGIPYQNAIVW
                     QDTRTAALCDVLSRRHGGQDRFREKTGLPISVYFSGIKLPWLFDQVDGLYEEALRGNL
                     MFGTIDSWLIWNMTGGITGGKHYTDVTNASRTMLMNLKTLSWDSEILEELDIPVQILP
                     EIKPSSGYFGAYHKNGAVIPISGVFGDQQAALFGQTCFSAGEGKNTYGTGCFLLLNTG
                     SVPVESKNGLITTVGFKIEDRPPVYCLEGSVANAGSLVQWMRDNLKMINSSAEIEALA
                     REVPDNGDVYIVPAFSGLFAPYWRSDARGIIAGLTRFSNRGHLARAVLEATAFQTMEV
                     VEAMKKDSGVDLSML"
     gene            1316..2323
                     /locus_tag="PKV59_05315"
     CDS             1316..2323
                     /locus_tag="PKV59_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQP46206.1"
                     /translation="MESIIYHDEKVIDVYLSIPFSSYLTLFGASMLPNFKIGVGTIAW
                     GNQLIWDYGIAYDEQSLYEVFSRSLQNGIRFFSTSEAFTDGDSERILGRFAAKYPEQA
                     FISTKYVPRPWRIRRSDFLRAMNASLARLQLRRLDLYQIGQPTGLMGIPMLVECAAEA
                     LDTGMVKNIGLSGFKANEIEQFSDLLNRLGGAVSCVETEYNLLNREIETNGVLKICQD
                     LHIPIIAQCPLAMGLLTGKYLNEPIVNGLRRKIMQRYPANGLNGLIRLMNLIGSEHEG
                     RNSAQVALNWLIQKKVIPIPGIKNVEQLDQNRTVIEWDLKPEEVTRLDDYSAGLAVGT
                     E"
     gene            complement(2382..3164)
                     /locus_tag="PKV59_05320"
     CDS             complement(2382..3164)
                     /locus_tag="PKV59_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HQP46207.1"
                     /translation="MAISFNSFRSGQYQIETDLYQGPLDLLLDLIEKAELDITVLSLA
                     QVTDQFLEYIKNMEGDNPAEVSAFVVIAARLIQIKSSALLPHSETDIRELDEDSGEAL
                     AQQLILYRRFKQLADWLAQREDLNYHSYERMGTPDIGIEAPLDLSNLTVIHLATIAGR
                     LFAKKPEQPELATMISQPRITIGQRIQLLIGKVREKRKISFRKLLSSGSKVEAVVTFL
                     AMLELIKRNVISVNQDNLFADIEIRASDESDREQSLVSEFGE"
BASE COUNT          943 a          840 c          817 g          909 t
ORIGIN      
        1 agcatactca aatccactcc ggaatctttt ttcatcgctt ccacaacctc catggtttgg
       61 aaagccgtag cttccagaac cgctcgggcc aaatgtccgc ggttggaaaa tcgagtcagc
      121 ccggcgatga ttccgcgtgc gtcgctgcgc cagtacgggg cgaacaaacc ggaaaaagcg
      181 ggaacgatat aaacatcgcc gttgtccgga acttcccgtg ccaaggcttc gatttctgcg
      241 gacgagttga tcatcttcag attatcccgc atccactgca ccaaggatcc ggcattggca
      301 accgaacctt ccagacaata aaccggaggg cggtcttcga ttttaaaacc gacggtagtg
      361 atcaatccgt ttttgctttc caccggaacg gatccggtgt tgagcagcag aaaacagccg
      421 gttccatacg tatttttccc ttcgccggcg ctgaaacagg tctgaccaaa cagagcggct
      481 tgctggtcgc cgaaaacacc gctgatggga ataacagcac catttttatg ataagcgccg
      541 aaataaccgg aagacggttt gatctctggt agaatttgaa ccggaatgtc caactcttcc
      601 aggatttcgc tatcccaaga aagcgttttc aggttcatca acattgtccg agaagcgtta
      661 gtgacatcgg tgtaatgttt gccgccggtt attccaccgg tcatattcca gattaaccag
      721 gaatcgattg tcccgaacat taaatttccg cgcaaagctt cctcataaag accgtcaacc
      781 tgatcaaaaa gccagggtaa cttaatcccc gaaaaataaa ccgaaatcgg caatccagtt
      841 ttctcacgaa accgatcctg accgccatgc ctgcgagata acacatcaca gagagcggca
      901 gtgcgagtat cttgccaaac gatggcgttc tgataaggga ttccagtata tcgattccaa
      961 actacggtcg tttcacgttg gttggtaatg ccgatcgcgg cgatatctcc cggctccgca
     1021 tcggctttgc gaacggcatt ttcgaaaata gtttcgacgt tccggagaat ttgaatcgga
     1081 ttgtgttcca cccaaccggg ttttggcaga atttggtcat gttccatttg gtcgctcgaa
     1141 acgatctctc cggcttgatt aaacagaata caacgactgc tggtagtgcc ttggtcgatt
     1201 gccgcaatat atttcgtcat ggatgcccct ttttcccccg cttttaaaac cttgagattc
     1261 tattccaatt ataaagagat gttttatggt tccgagcgga aaccttttat aatgattgga
     1321 aagtatcatt tatcatgacg aaaaagtgat tgatgtatat ttatcaatcc cgttttcaag
     1381 ttatctgact ttgttcggag cgtccatgct gccaaatttt aaaataggtg tcggaacaat
     1441 tgcctgggga aaccaactga tttgggatta cgggatcgct tatgacgagc agtcgctgta
     1501 cgaagtgttc agccgctctc ttcaaaacgg aattcgtttt ttcagcacca gtgaagcatt
     1561 tacggacgga gacagcgaac ggattctcgg cagatttgcg gcaaaatatc ccgaacaagc
     1621 tttcatatcc accaagtatg tgccgcgtcc atggagaatt cgccgcagtg attttttacg
     1681 ggcgatgaat gcttcgcttg ctcggttgca gctgcgccgg cttgaccttt atcaaatcgg
     1741 tcagccgacc ggattgatgg gcattccgat gctggttgaa tgcgcggcgg aagcgcttga
     1801 taccggtatg gtgaaaaata tcgggctgtc cggtttcaaa gcgaatgaaa ttgaacaatt
     1861 cagtgatctg ctgaatcgat tgggaggtgc ggtctcttgt gttgaaacgg aatataatct
     1921 tttgaatcgc gagatcgaga ccaacggagt gcttaagatt tgtcaggatt tacatattcc
     1981 gattattgca caatgtccat tggcgatggg tttgctgacc gggaaatatc tcaacgaacc
     2041 gatcgttaat ggtctgagac ggaaaattat gcagcgttat ccggcgaacg gattaaacgg
     2101 attaatccgt ctgatgaacc tgatcggatc cgagcacgaa ggtcgtaaca gcgcacaggt
     2161 agcattgaat tggctgattc aaaaaaaggt tatcccgatc ccgggtatca aaaatgttga
     2221 gcaattggat caaaaccgaa cggttatcga atgggacttg aagccggaag aagtcaccag
     2281 actggatgat tattcggccg gtttggctgt cgggacggaa tgaacgcatt actcctaaaa
     2341 atcgcatctg tatgattcca atcggtggga aaaaggaaaa attattctcc gaattcggag
     2401 accaaagatt gctcccgatc gctttcatcc gaggcgcgaa tttcgatatc ggcaaaaaga
     2461 ttatcctgat taacggaaat aacatttcgt ttgatcagtt cgagcatcgc cagaaaggtg
     2521 accaccgcct ccactttcga tccggatgat aagagttttc ggaaagaaat cttccttttc
     2581 tcgcgaactt tgccgatcag cagttggatg cgttgcccaa ttgttatgcg cggctggctg
     2641 atcatggtcg ccagctcggg ttgttccggt tttttggcga ataacctgcc ggcgatagtg
     2701 gcaagatgaa taaccgtcag gttactcaaa tccaatggcg cttcgatacc gatatccggt
     2761 gtacccatcc gctcatagct gtgataattt aaatcttccc gttgtgccaa ccaatctgcc
     2821 aactgtttaa accggcggta caaaatcaat tgttgcgcca atgcttcgcc ggaatcttca
     2881 tccaattccc gaatgtccgt ttccgaatga ggcagaagtg cggatgattt aatttggatt
     2941 aagcgggcgg cgatcaccac aaaagcggaa acttcggcgg gattatcccc ctccatgttc
     3001 ttgatatatt ccagaaactg atctgttacc tgtgccaagg aaagaaccgt aatatccagc
     3061 tccgcttttt caatcaaatc caacagcaaa tcaagcggtc cctgatagag atcggtttct
     3121 atctgatatt gtccggaacg gaaagaattg aaagaaatgg ccatctggtt tgaattttaa
     3181 cacagccgtc ggaagcgaca cccgaaagca cagaaaaagt gtaaaaaaga gggaagccct
     3241 ctcacatctc cttcccggca ggcttgccga aaatcaacca tcactccggc tacccttcgg
     3301 ataacatatt caagagcatt ctactaatca acttcattcg aaaatctcat ttcttcaaaa
     3361 agggacttga gagggaagcc ctctcatatc tctcccagca ggcttgccga aaatcaagtc
     3421 ccatcccggt ttgcggtcgc accacctgtt caacgacatt ctgataactg atttcattcg
     3481 agaaccccat tttttcaaaa agggacttg
//
LOCUS       DAOMIV010000187         3495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28349_length_3495_cov_2.991279, whole genome shotgun sequence.
ACCESSION   DAOMIV010000187 DAOMIV010000000
VERSION     DAOMIV010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28349_length_3495_cov_2.991279"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1176)
                     /gene="metA"
                     /locus_tag="PKV59_05325"
     CDS             complement(253..1176)
                     /gene="metA"
                     /locus_tag="PKV59_05325"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HQP46208.1"
                     /translation="MPVNIPDNLPAAETLENENIFVMKGQRATHQDIRPLQILILNLM
                     PTKIETEIQLLRLLSNSPLQINVEFLQMVTHESKHTSQDYLDYFYKTYDQISQNKYDG
                     FIITGAPVENIPFEAVDYWEELCKLMSWSRRNVFSTMHICWGAQAGLYYHYGINKYPL
                     DKKISGIFSHYPVVRNHPLLRGFDEIFYMPHSRYTAVRREDVEAHPELQVLAVSNEAG
                     LAISATRDGRQIFIQGHAEYDVMTLANEYRRDLKAGINPVMPENYFPGNSPKAAPIMY
                     WRSHANLLFTNWLNYYVYQQTPYDIDKISQV"
     gene            complement(1179..2468)
                     /locus_tag="PKV59_05330"
     CDS             complement(1179..2468)
                     /locus_tag="PKV59_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HQP46209.1"
                     /translation="MAANHEWKFETKQLHVGQEQADPASDARAVPIYQTTSYVFQNTD
                     QAAARFSLAESGNIYGRMTNTTQEIFEKRMAALENGTAALAVASGAAAVTYSFLNLAK
                     AGDHIVSAATIYGGTYNLLQHTLPDYGISATFVNADEPGGFEKAIQPNTKAVFIETIG
                     NPNATLIDIEKVAEIAHAHRIPLIVDNTFATPYLVRPIEYGADIVVHSATKFIGGHGT
                     AIGGVIIDSGKFDWAGSGKFPEITEPNPSYHGISFVEAAGPAAFAARARAILLRDTGA
                     TLAPLHAWLFLQGLETLSLRVERHVENALKVVEFLHAHPKVERVNHPSLPDHPHHQLY
                     RKYFPHGGASIFTFNVKGDEKAAKQFIDRLQVFSLLANVADVKSLVIHPASTTHAQLN
                     AQELAEQDIQLNTVRLSIGTEHIDDILYDLNQALEGI"
     gene            complement(2686..>3495)
                     /locus_tag="PKV59_05335"
     CDS             complement(2686..>3495)
                     /locus_tag="PKV59_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQP46210.1"
                     /translation="AHMPRVDAIGVSSAGIFINNETKVASLFLKVPPEDFKKKVRTIY
                     IDAAREIGENIPLVVANDGDVTALAGSMSLQKNGVLGIAMGTSEAGGYVDLEGKITGW
                     MNEFAFSPIDLSEYAMVDEWSGDKGCGVKYFSQDGVIKLCPTAGIDLKEEETPAEKLK
                     TVQMLMEENDPRAVKIFDSIGVYLGYALKLYSMFYEGNEVLLLGRVVSGKGGERMLDM
                     ANKVLRTEFPNLNLNVSLPSEKTRRVGQAVAAASCQRSKRRKNNCHKVSKI"
BASE COUNT          885 a          902 c          750 g          958 t
ORIGIN      
        1 ataacgacgg ttggttgtcg gaagacggtt ggattatcgt tcaaatccat ccggttaaac
       61 tggaaccgtt agagcttaac aatttcattc gtttcgacga acgaaaatac ggcagcaccg
      121 ctttgctgtt ctaccggcgc aaaccgctcc caacgaatgc gacttagtta ccgcacatga
      181 cgatgcaaac gtccgattct ccgctgattg aagagaccgt ccgataattt tccttttcgt
      241 accgatacac cgttatacct gactgatttt atcgatatca tacggcgttt gctgataaac
      301 ataataattc agccaatttg tgaataacag gttggcatga ctgcgccaat acataatggg
      361 agcagctttg ggactgtttc cggggaaata attttccggc atcactggat tgattccggc
      421 tttcagatcc cgacgatatt cattcgccag tgtcatcacg tcatattcgg catgtccctg
      481 aataaaaatc tgtcgtccgt cgcgagtagc cgatatcgcc aaccccgctt cgttcgagac
      541 cgccaaaacc tgcaattccg ggtgtgcttc cacgtcttcg cgccttaccg cggtataccg
      601 ggaatgaggc atataaaaga tttcgtcgaa tccgcgcagc aacggatgat tgcgcaccac
      661 gggataatga gaaaatattc ccgaaatctt cttatccaag ggatatttgt tgatcccata
      721 atggtaatat aatcccgcct gggctcccca gcaaatatgc attgtggaaa acacgtttct
      781 gcgtgaccaa ctcatcagtt tgcacagttc ttcccaataa tcgaccgctt caaaaggaat
      841 attttcgacc ggtgcgccgg tgatgatgaa accgtcatat ttattttgac tgatctgatc
      901 atacgtctta taaaaatagt ccagataatc ctgagaagta tgtttggact cgtgcgtcac
      961 catttgtaag aattcgacat tgatctgtaa aggactgtta ctcaataatc gcagtaattg
     1021 aatttcggtc tcgattttgg tcggcattaa attcaatatc agaatttgca acggccggat
     1081 atcctgatgc gttgcccgtt gccctttcat gacaaaaata ttttcatttt ccagcgtctc
     1141 ggcagccgga agattatccg gaatattcac aggcataatt aaatcccttc caatgcttga
     1201 ttcaaatcat acagaatatc atcgatatgt tcggtcccga tggaaagccg aacagtattc
     1261 aactgaatat cctgttctgc caattcctga gcatttaatt gggcatgggt tgtgctggcg
     1321 ggatgaatga ccaggctttt cacatccgct acattcgcca acagagaaaa tacttgcaaa
     1381 cgatcgataa attgtttggc agccttttca tcgcctttga cattgaatgt gaaaatcgaa
     1441 gcaccgccgt gaggaaaata ttttcggtaa agttgatggt ggggatgatc gggaagggac
     1501 ggatggttca cacgttccac tttcggatga gcatgcaaga attcgacaac cttcagcgca
     1561 ttttcgacat ggcgttccac cctcaggctg agtgtttcca aaccctgaag aaacagccag
     1621 gcatgcagcg gcgccagcgt cgcacccgtg tcccgtaaca ggatcgcccg tgccctggcc
     1681 gcaaaagccg ccggaccggc cgcttcgaca aaactgatcc cgtgataact ggggttgggt
     1741 tccgttattt cgggaaattt tccgctgccg gcccaatcaa acttgccgct gtcaatgatt
     1801 acaccgccga tcgccgtacc gtgaccgccg ataaattttg tcgcggaatg aaccacaatg
     1861 tccgctccgt attcgatcgg ccgtaccaaa taaggtgtgg caaacgtatt atccacaatc
     1921 aacgggatgc gatgagcatg agcgatttct gccacctttt cgatatcaat caatgtcgca
     1981 ttggggtttc caatcgtctc gataaagacg gctttggtat tgggttgtat cgctttctcg
     2041 aaaccgccgg gctcatcggc atttacgaaa gttgcggaaa ttccgtaatc cggaagcgta
     2101 tgttgcaaaa gattataagt tccgccgtaa attgtcgctg cagagacaat atggtcgccg
     2161 gcttttgcta aattcaggaa gctgtaagta acggcggcag cacctgaagc cacagccaat
     2221 gcagccgtac cgttttccaa agcggccatc cgcttctcaa atatttcctg cgtggtattg
     2281 gtcattcgtc cgtagatatt cccgctctca gccaggctga atcgggcggc cgcctgatcg
     2341 gtattttgaa atacataaga cgtcgtctga tagatcggca ccgctcgggc atccgaagcg
     2401 ggatcggcct gctcctgccc aacatgcaat tgttttgttt cgaatttcca ctcatgattt
     2461 gccgccattt ttaacctctt tcttttcgat cgaaaacctt tccgaaaatt aatataaaaa
     2521 aggacctcaa taagcgatat cgaggtcctt ctcatcttct ctgcatagcc ctgactaagc
     2581 gatcagatca agtcacctcg cttctgtacc atgggcatgg gcatcaaata aatttggttt
     2641 gtcatagtaa aaataagttt atattccccg tatcattttt gtcaatcaga ttttcgaaac
     2701 cttatgacaa ttatttttcc ttcttttcga tctttggcaa ctggcagccg cgaccgcctg
     2761 cccgactctc ctcgtttttt ccgaaggcag agatacatta agattcaaat tcggaaattc
     2821 ggttcgcaag accttgtttg ccatatccag cattctctct ccgcccttgc cggaaaccac
     2881 ccgccccagc agaagcactt cattcccttc gtaaaacata ctgtagagtt tcagagcgta
     2941 tcccagataa accccgatcg agtcaaaaat tttcaccgca cgcggatcat tttcctccat
     3001 cagcatttga actgttttca gcttttccgc cggcgtttcc tcttctttca aatcgatccc
     3061 ggctgtgggg cacagcttaa tgacaccatc ttggctgaag tattttacgc cgcagccctt
     3121 atctcccgac cattcgtcaa ccattgcata ctcggaaaga tcgatcggtg aaaaagcgaa
     3181 ttcattcatc caacccgtaa ttttgccttc gaggtccaca tatccaccgg cttcgctggt
     3241 tcccatagca atccccaaca ccccgttttt ctgcagactc atcgaaccgg ccaaggcggt
     3301 cacatctccg tcgttggcaa ctaccaatgg gatattttct ccgatttcac gcgcggcatc
     3361 gatataaatt gtacggacct tttttttgaa atcttccggc ggaaccttca agaacagcga
     3421 ggcgactttc gtctcgttgt tgataaaaat tccggctgat gaaacgccga tcgcatcgac
     3481 acgcggcata tgcgc
//
LOCUS       DAOMIV010000188         3485 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28463_length_3485_cov_4.000583, whole genome shotgun sequence.
ACCESSION   DAOMIV010000188 DAOMIV010000000
VERSION     DAOMIV010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28463_length_3485_cov_4.000583"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /gene="feoB"
                     /locus_tag="PKV59_05340"
     CDS             complement(<1..1786)
                     /gene="feoB"
                     /locus_tag="PKV59_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HQP46211.1"
                     /translation="MIDSHIDSEQDEFIPKADHTHVGTIADLKSDNNPNGLRLTFALA
                     GNQNSGKTTLFNYLTGSNQHVGNWPGVTVEKKTGKVKKYPGITLVDLPGIYSLSPYTD
                     EEMITRNYLLDEKPDAIINIVDANNLERNLYLTLQLAELGIPMVIALNMMDEIAANGD
                     VIDVEGLERDLGIPVVPIVARNGSGVEDLIRRAIGVAMHREQPPIRDICEGEVHKAIH
                     SIGHIIEPKARARGYSARFVSTKLIEGEDPIKQAIGLTPEEEKIIDEIISEMEKATGL
                     DREAAIADSRYRFITKVCSENFKRRRKIGEQSASDKIDKVLTGKYSALPIFLAIMLFV
                     FWLTFGPIGSRLSEGFSELIDTGIGIVDHALTYAGVSEWLQSLVVDGILGGVGSVLNF
                     LPVILILFICLSVLEDCGYMARAAFIMDRLLRKIGLSGRSFIPFMMGFGCTVPALMAT
                     RTLENKRIRYLTVLLTPFMSCGAKVPVYTLFIAAFFAKGKILVIFSLYVIGILVAVLM
                     GLILKSTLFRGTPAPFIIELPPYRWPTPHNVAIQVWEKIKDFLERAVTIILLATVVIW
                     FLGSFDPKFYWTADSSQSILATLGGFFAP"
     gene            complement(1779..2033)
                     /locus_tag="PKV59_05345"
     CDS             complement(1779..2033)
                     /locus_tag="PKV59_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HQP46212.1"
                     /translation="MIKTLDQFVPGDQGEVVKVGGQGAIRRRLLDMGITPGTRILLIK
                     TAPMGDPLEITLRGFNLSIRKEDARYIELKMDSPEEESND"
     gene            2345..3010
                     /locus_tag="PKV59_05350"
     CDS             2345..3010
                     /locus_tag="PKV59_05350"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF005302.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HQP46213.1"
                     /translation="MLLEEIRSEIIEYGKKLIDNRLTKGTGGNLSACDRKTGLMAITP
                     SGIDYYQIKPEQIMLIDIQSGEIKDGTAIPSSEVDMHRILYKYREDLNAVIHTHSTFA
                     AALSCLNITLPPVHYLVAYAGVDVRCAKYATYGTVALAKNAFEAMKDRKACLLANHGL
                     LAGGTDLANAFNITEEIEFCCELYYRAKSIGEPVILSKEEMVRMVDRFKNYGKRIEEH
                     EQI"
     gene            complement(3095..>3485)
                     /locus_tag="PKV59_05355"
     CDS             complement(3095..>3485)
                     /locus_tag="PKV59_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46214.1"
                     /translation="GTFAGAFNDYICRELGYKTDLPYAFSTDLWKTWSYKEYENKYVQ
                     LEEVLRKTMVRNKFMKVWVLNGYYDMATPFYASEYVFSHLQLDPVLQPNLSLTYYEAG
                     HMMYIHKPSLEKFRKDAEFFFENALKA"
BASE COUNT         1014 a          815 c          722 g          934 t
ORIGIN      
        1 ggggcgcaaa aaatccgccg agggttgcca aaatgctctg tgaagaatcc gcggtccaat
       61 agaatttcgg atcaaatgag ccgaggaacc agatgactac ggtagccaac agaataatgg
      121 ttaccgctct ttcgaggaaa tcctttattt tttcccaaac ctgaattgcc acattatgcg
      181 gcgtcggcca gcgataagga ggaagttcga taataaaagg agccggcgtt ccgcgaaaca
      241 aagtgctttt taagatcaag cccatcagaa cagcaacaag aatcccgatc acataaagtg
      301 aaaaaataac aagtatcttc cctttggcaa aaaaagctgc gataaacagc gtataaaccg
      361 gtacttttgc gccacacgac ataaagggtg tcaacagaac cgtcaaataa cgaatccgtt
      421 tattttccag cgtccgtgtc gccatcaaag ccggtacggt acatccgaat cccatcataa
      481 acggaataaa cgaacggccg gacaagccga tttttcgcag caatcgatcc attatgaatg
      541 ccgcccgagc catatacccg caatcttcca aaacggataa acagatgaaa aggatcagga
      601 taaccggtaa gaaattcaac acacttccga ctccgcccaa tattccgtcg acaaccaggc
      661 tctgtaacca ttccgacaca cctgcatacg tcagagcatg atcgacaatg ccgattccgg
      721 tatcgattaa ctcggaaaac ccttcactta atcggctgcc gatcggtcca aatgtcagcc
      781 aaaaaacaaa caacattatc gccaaaaaaa tgggaagagc tgaatatttg cctgttaata
      841 ctttatcgat tttatccgaa gccgattgtt cgccgatttt tctccttcgt ttgaaatttt
      901 cggaacagac tttggtgata aaccggtagc gggaatccgc gatcgccgct tcccggtcaa
      961 gtccggtagc tttttccatc tccgatataa tttcatcgat gattttttct tcttcgggag
     1021 tcaggccgat ggcttgcttg atcgggtctt caccttcaat caacttggtg gaaacaaagc
     1081 gtgcagaata tcctcgcgct ctcgcttttg gctcgataat atgaccgatc gaatggatcg
     1141 ctttatggac ttccccttca caaatatcgc gaatcggagg ctgctcgcga tgcatggcga
     1201 caccaatggc acggcggatc aaatcttcca ctccgctgcc gtttcttgcc acaatcggta
     1261 caaccggaat tccaagatct ctctccaacc cctctacatc gatcacatca ccgttggcag
     1321 cgatttcatc catcatattc aatgcgatga ccatcggaat tccgagctcc gccaactgaa
     1381 gcgtcaaata caaattgcgt tccaggttat tcgcatcaac gatattgata atcgcatccg
     1441 gcttttcatc gagaaggtaa tttcgggtga tcatctcctc atctgtataa ggagaaagcg
     1501 aataaattcc gggcagatcc accaatgtga tccccggata ttttttcact tttccggttt
     1561 ttttctcgac ggtcacaccg ggccaatttc cgacatgttg gttggagccg gttaaataat
     1621 tgaaaagggt tgttttaccg ctgttttggt ttcccgccag tgcaaaagtc aggcggagac
     1681 cgttggggtt gttatcgctt ttaagatcgg cgatcgtgcc cacatgagta tgatcagcct
     1741 ttggaataaa ctcgtcttgt tcggagtcaa tatgcgaatc aatcattcga ctcctcctcg
     1801 ggtgaatcca ttttcaactc gatataacga gcatcttctt ttcgaatact cagattaaat
     1861 ccgcgtaagg tgatttcgag gggatcgccc atcggggcgg ttttgatcag gaggattcgg
     1921 gttccgggtg tgatacccat atcgagtaac cttcgccgaa ttgctccctg cccaccgact
     1981 ttgacgacct caccttggtc gccgggaaca aactgatcta atgttttaat catctctctg
     2041 cctttcggaa catcttcaag ttcctgcttg cagggaatat tctatcactt gcgacgaaag
     2101 cggaaagaac caattgagtc cgattattac cggatgaata cttccgcaaa gagagtcaaa
     2161 attctgactg cggcaaattc gtcgaaaccg ctactttccc gttccataaa aaacgatttt
     2221 tccctttata ccggaataga aaatcataat catcggtcag tgcggattat ctcggttata
     2281 aaataagaaa taaagatttt tccggaacaa gtcaaacatc ccggaagata gctcaggagg
     2341 ttatatgctg ttggaagaaa tccgatcgga aattatcgaa tatgggaaaa aattaatcga
     2401 caatcgttta accaaaggaa ccggcggaaa cttaagtgcc tgcgatcgaa aaaccgggtt
     2461 gatggcaatt acccccagtg gaatcgatta ctatcaaatc aaaccggaac aaattatgct
     2521 catcgatatt caatcgggag aaattaagga tggaactgca atcccgtcca gcgaagtgga
     2581 tatgcaccga attctatata aatatcggga ggatttaaat gccgtaatcc atactcacag
     2641 cacttttgct gcggcgctct cttgtctcaa tataacctta cctcctgttc actatctggt
     2701 cgcttatgcg ggagttgatg ttcgatgtgc aaaatatgcg acttacggaa ccgttgcgct
     2761 tgcaaagaat gcctttgagg cgatgaaaga tcggaaagcc tgccttttgg caaaccatgg
     2821 gctgttagcc gggggtacgg atttggcgaa tgcctttaat atcaccgaag agatcgagtt
     2881 ttgctgcgaa ctatattatc gggcaaaatc gatcggtgaa cccgtaattc tttccaaaga
     2941 ggaaatggtc cgaatggtag atcggtttaa gaattacggt aagcggattg aagaacatga
     3001 acaaatttag cctcgttttt ttctgaaaaa attaaataaa aaaaagagac caatatcatt
     3061 ttccggtctc ttttataaat aatttaatcg attttcaagc tttcaaagca ttttcaaaga
     3121 aaaattctgc atcttttcgg aatttttcca acgatggctt atggatatac atcatatgcc
     3181 ccgcttcata ataagtgaga ctcaggtttg gctgcaaaac aggatcaagc tgtaaatggc
     3241 tgaacacata ctccgaagca tagaatggtg ttgccatgtc ataatatccg ttcagaaccc
     3301 agactttcat aaacttattt cgaaccattg tttttctcaa aacttcttcc aattgaacat
     3361 atttattttc atattcttta tagctccacg tcttccataa atcggtcgaa aatgcatagg
     3421 gcagatcggt cttataacct aattcccgac agatataatc attgaaagct cccgcaaaag
     3481 ttccg
//
LOCUS       DAOMIV010000189         3481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28515_length_3481_cov_4.762989, whole genome shotgun sequence.
ACCESSION   DAOMIV010000189 DAOMIV010000000
VERSION     DAOMIV010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28515_length_3481_cov_4.762989"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..1397)
                     /locus_tag="PKV59_05360"
     CDS             complement(222..1397)
                     /locus_tag="PKV59_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQP46215.1"
                     /translation="MMKTQKLDKDLMKKNNMKLVLACINEADEISKAQIAEKVQMSIM
                     SINRITDDLIQLGFVCETDRTKENSIGRPPMMLSLNRDNLLSLSVSLEKDTMRVGLVD
                     PFGEIKAIREYTITSSRFDPQEVLKQLAMNLQRFIDSQPDRKILPVVGIVLPGIVDNK
                     KGRMEFSSNLKWHNVQISKILKEYFPSKDFVVENDIKAIAIAENRFGASKQCHNSVVM
                     NIGNGIGAAVIIDNEIYRGKNNMAGEIGHIIINPVGQMCECGQIGCLQTKIAEWAILR
                     EAQSVEADITLEGLFARYDEKADWAVKLIDSVVDYISISINLLANTYSPEMILLCGSM
                     IQQNKKLRELIEKNYRRHLNDYIKNSFQLKFESFGRDGHIIGGSIIAYQRYIDEVIK"
     gene            1609..2352
                     /locus_tag="PKV59_05365"
     CDS             1609..2352
                     /locus_tag="PKV59_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HQP46216.1"
                     /translation="MGNILVEVCCGSADDAVESFQAGADRVELNSDLFHGGLTPSIGE
                     LIVAKKHTGLKIMTMVRPREGGFCYTENEFATAVEDAKLLLAHGSDGLVFGFLKSDGT
                     IDVERTKIMKQIAGDKTTVFHRAIDVVPDWKSALDTLISLGITRVLTSGQESNVFFGI
                     ETVKEMITYADGRIEILPGAGITLKNIDRIVSETGCDQVHVAHYKPYSDSSVNNNREI
                     FYGGALYPPEDRFNMVDRDYISAIVNMTN"
     gene            complement(2491..3237)
                     /locus_tag="PKV59_05370"
     CDS             complement(2491..3237)
                     /locus_tag="PKV59_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQP46217.1"
                     /translation="MKITVLGTNGWFDTDTGSTNCVLLQTSDYSVIMDAGFGITKIKT
                     RVDFSKPVYLFLTHLHLDHVIGLHALDYFNFEQPLRVITPIGGTDDLKDLLRSPFSAA
                     WDSHVYPIEMIDAKNLEKTNLPFKVEALPLVHAVPDTGYRFEIDGKIIAFVIDTAYCE
                     NAVKLAKNADLLITECGFYPGTVNTVSGHMTPETAAKLASDSNAKQTVLIHFGANAFE
                     TIDKRSRAVDTGRKVYPGLIMGLDNMEFIL"
BASE COUNT          994 a          765 c          694 g         1028 t
ORIGIN      
        1 gcatgaatgt ataacaaaac tccacgcaaa ttctttcaac ttccggcaat tctctgataa
       61 accgagctgt ttcaactccg gacttaccga aagaaggaag tcgttgaaga taacctcgat
      121 gaatcgtttg aattttttct tcactgaaaa gcaaggtcat tttcctaccg tccttccttt
      181 tccgtctaag gcacccgttc gttgactctt aaaaataaaa atcatttaat gacctcatca
      241 atatagcgtt gataagcgat tattgatccg ccgataatat gtccgtctcg gccaaagctt
      301 tcgaatttca actggaacga atttttaata taatcgttta aatgccttcg ataatttttt
      361 tcgatcaatt ctcttaactt tttattctgt tgaatcatgg acccgcaaag caagatcatt
      421 tccggtgaat aggtattggc aagcagatta atggagatgg aaatataatc gacgacactg
      481 tcaataagtt ttacagccca atccgctttc tcatcatagc gggcaaataa accttcgagc
      541 gtgatatccg cttcgacact ttgcgcttcc ctcaggatag cccattcggc gatttttgtc
      601 tgaagacaac cgatttgacc gcattcacac atctggccta ccggattgat gataatatgc
      661 ccgatttcgc cggccatatt attcttaccg cgataaattt cgttatcaat aatgaccgcg
      721 gcaccgatac cgttcccgat attcatcaca accgaattat ggcattgctt cgatgcgccg
      781 aaacgattct cagcgatcgc gatcgcttta atgtcatttt caaccacaaa gtcttttgaa
      841 ggaaaatatt cttttagaat tttactgatt tgaacattat gccatttcag gttggatgaa
      901 aactccatcc ttcctttttt attatccaca attccgggta acacgattcc caccaccggt
      961 aagatcttcc gatcgggctg gctgtcgatg aatcgttgga gattcattgc cagttgcttc
     1021 aaaacttctt gcggatcgaa tcgactggaa gtgatcgtgt attcccgaat agcttttatc
     1081 tctccgaaag gatcgaccaa tcccacccgc atcgtgtcct tttcaaggct gacactcaat
     1141 gaaagcaggt tgtcgcgatt gagagaaagc atcatcggag gccttccgat tgaattctcc
     1201 ttggtgcggt ccgtttcaca aacgaaaccc aattggatca gatcatcggt aatccgattg
     1261 attgacataa tgctcatttg cactttttcg gcaatttgcg ctttggagat ttcatcagcc
     1321 tcattaatac aggccaacac gagcttcata ttatttttct tcatcagatc tttatctaat
     1381 ttctgcgttt tcatcatgaa ggcctcttta aatatcactg atcggatcat tcattacggt
     1441 ttcaatataa ttattatcgt catgttaata ttaaagctgt aatacttcgt gcttatttct
     1501 attttatcaa actttcaaaa gctttacaaa ccgattttta ccaaaatttc cggttacaaa
     1561 gtataatgac acaaaactag aagaataata ttgcaagcga ggttaataat ggggaacatt
     1621 cttgtcgaag tctgctgcgg cagcgcagat gatgcggttg aatcttttca ggcgggggcg
     1681 gatcgagtcg aattgaacag cgatcttttt cacggcgggt taaccccttc gatcggcgaa
     1741 ctgatcgttg caaaaaagca taccggcttg aaaatcatga caatggttcg gcccagagaa
     1801 ggaggattct gctataccga aaatgagttt gccacggcgg ttgaggatgc aaaattatta
     1861 ttagcccacg gttcggacgg attggtgttc ggttttctca aatcggatgg gacaatcgat
     1921 gttgaacgaa caaaaattat gaagcaaatt gccggcgata aaacaaccgt ttttcatcgg
     1981 gcaattgatg ttgtgccgga ctggaaatcc gccttggata ccctcatctc gcttggaata
     2041 accagggttc tgaccagcgg acaggaatcg aatgtctttt tcggaatcga aacggtaaaa
     2101 gaaatgatca cctatgctga cggaagaata gaaattttgc cgggagcggg cataacattg
     2161 aaaaatatcg accggatcgt cagtgaaacc ggatgcgatc aggtacatgt tgcccattac
     2221 aaaccgtatt cggattcttc tgtcaataat aatcgcgaaa tattctacgg cggtgcatta
     2281 tatccgccgg aagatcgttt caatatggtg gacagggatt atatttccgc catcgttaat
     2341 atgacaaact aaaaatattc aggcagtata aatatttttc agtttctcct ccaaaccatg
     2401 agggcgaatc gccctcatgg tttttaatcc cggatcaaac tccgcatcga atcaatattt
     2461 atttggataa aacagtccgg cgatatcgct tcaaaggata aattccatat tatcgagtcc
     2521 catgatcaat cccggataaa cctttcttcc cgtatccact gcccggctcc gtttatcgat
     2581 cgtttcaaag gcgtttgcgc cgaaatgaat caagacagtc tgttttgcgt ttgaatcaga
     2641 cgccaatttc gctgccgttt ccggcgtcat atgacccgat accgtattga cggttccggg
     2701 ataaaaaccg cattccgtaa tcagcaaatc ggcgtttttt gccaatttga ccgcattttc
     2761 acagtaagcg gtatcaatta cgaaggcaat aatttttcca tcgatttcaa atcggtaacc
     2821 ggtatccggt acggcatgta ccagaggcaa ggcttcgact ttgaaaggaa gattcgtttt
     2881 ttccaaattc ttcgcatcaa tcatctcaat cggataaaca tggctatccc atgctgcgga
     2941 aaaaggggat cgcagcaagt ctttcagatc atccgttcct ccgatgggcg taatcactcg
     3001 caacggttgt tcgaaattga aataatcgag cgcatgtaac ccgatcacat gatccaaatg
     3061 gagatgggtc agaaaaagat agaccggttt tgagaaatca actctggttt taatttttgt
     3121 aatgccaaag cccgcatcca tgattacgga ataatcggat gtctggagca acacacagtt
     3181 tgtgctgccg gtatctgtat caaaccaacc gtttgtccct aaaacagtta ttttcatgat
     3241 ttcactccta agctatttct gcatagaatt ttacatcaag attattcacg gaatttgtcc
     3301 gccttgaaaa agtctgcgta tcatttttcc ttgactatgg aaatttgata cgccaacttt
     3361 tataaaacgc agatttgact gatcacagaa aaaaggtaaa ataaaaaaaa tagaaaatac
     3421 tatttatcct ttggggcagg gtgaggttgc cgatgcagcc gattcccgat cggtggtaat
     3481 a
//
LOCUS       DAOMIV010000190         3469 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28663_length_3469_cov_4.405390, whole genome shotgun sequence.
ACCESSION   DAOMIV010000190 DAOMIV010000000
VERSION     DAOMIV010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28663_length_3469_cov_4.405390"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PKV59_05375"
     CDS             complement(<1..694)
                     /locus_tag="PKV59_05375"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQP46218.1"
                     /translation="MFRKVPKSPNYPETGSSLKMDHELQSSGKTLRFQYRNSELMRLD
                     HYLVEVLPDYSRSRLQQFITEGRISVDGKPAGKSGLKLNLGQEIEITLPEEKTSELIP
                     ENIPLDILYEDAQVIVVNKPAGMVVHPSAGHESGTLVHAVLAHCQDLKGFGGEIRPGI
                     VHRLDRDTSGVIIIAKNEQAHIFLQKQFKSHKIDKRYLALVDGKPPTPNGRIETPIAR
                     DPIHRQRMAVLPA"
     gene            complement(639..1268)
                     /gene="lspA"
                     /locus_tag="PKV59_05380"
     CDS             complement(639..1268)
                     /gene="lspA"
                     /locus_tag="PKV59_05380"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQP46219.1"
                     /translation="MNKRGKVYLKLFSIAIGVIFIDQLTKWLVRTNLLIRETWMPISW
                     LEPYFRFIHWKNTGVAFGLFQGMGWILTSFGLVVILIIIFFYKPVFNGPAFWQIALGL
                     QLGGAIGNLLDRINPNVGYVVDFIWIGNFPVFNLADFAIVSGAIVMLIGIWYTEERAE
                     KKEGNKEGKSFETSSPIGSITDNTSRVPADPDVSKGSQITELSRDGKQP"
     gene            complement(1446..2162)
                     /locus_tag="PKV59_05385"
     CDS             complement(1446..2162)
                     /locus_tag="PKV59_05385"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQP46220.1"
                     /translation="MKKSSLLMIVFLSMMILSLGMAGSVQAQSDQTMTVSGTGTVLLK
                     PDIAIINVSISTEKESVQEALDENTKSVKLVKSALTEMGIPEEDIKTTSYSLYSTQKY
                     YRDETAGMDEYIFSVFYGFEIKLRDISKLNDVLDACINNGVNSINSVSYDSSLREDTY
                     MQARDLAIDDANANAQQIAQKLGVTVGNIKSFQVRDYPTDYGAMYGMGGGGYGGTAPL
                     ESGSLKIVVTVDITYEFSLP"
     gene            complement(2213..2923)
                     /locus_tag="PKV59_05390"
     CDS             complement(2213..2923)
                     /locus_tag="PKV59_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQP46221.1"
                     /translation="MNFKKILFLFIGTIALFGISVFKVSAQNENPAIIKVKGTGEVTL
                     NPDLALIDIAIATTADDSKTAIDENRKNALAIQQGLLEMGLKEDDIRTTEYILYSLSK
                     TDKDGNPIAGQYIYKVNLAYEIVVRDLSMLNDVLNFCIQNGANNLYGVNFDYSKRSEA
                     VAQARDLAIDHANVNAQQIAEKLGKKVDHLQTFSVLDNTEIPLISTKLVKDVSTAPID
                     SGFLTVTVSVDMEFALTD"
     gene            3230..>3469
                     /locus_tag="PKV59_05395"
     CDS             3230..>3469
                     /locus_tag="PKV59_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HQP46222.1"
                     /translation="MLDKIDAIEARYNELSNLIAENVEDYQKTAEWMKERSEIEEIVE
                     KGREYKDTLKRIEEDKALEKDPEMSELASTELDDLL"
BASE COUNT          991 a          805 c          679 g          994 t
ORIGIN      
        1 cggcgggtaa tacagccatt ctttgccggt gaatcggatc tcttgcgata ggcgtctcga
       61 tccgcccatt gggcgtcggc ggtttgccgt ctaccaacgc cagatatcgc ttatcgattt
      121 tatgagattt aaattgcttt tgcaaaaaga tatgtgcctg ttcatttttt gcgatgataa
      181 tcacccctga ggtatctcga tcgagtcgat gaacgatccc gggacgaatt tcgccgccga
      241 atccttttaa atcttgacag tgagccaata cggcatgtac caaagtcccg ctttcatgtc
      301 cagctgaagg atgaacaacc atgccggccg gtttatttac gacaataacc tgagcgtctt
      361 catacaaaat gtccagcgga atattttccg gaatcagctc ggaagttttt tcttccggca
      421 atgtgatttc gatttcctga cccaaattca gtttaagtcc gcttttaccc gccggcttgc
      481 catcgaccga tatccgtcct tccgtaataa attgctgcag acgggaacgg gaataatcag
      541 gcaatacctc gaccaaataa tgatccaacc gcatcaattc ggaatttcga tattgaaatc
      601 gcagagtttt tccggaggat tgaagttcat gatccatttt aaggctgctt cccgtctcgg
      661 gataattcgg tgatttggga acctttcgaa acatccggat ccgccggaac ccggctcgta
      721 ttgtcggtta tcgacccgat cggggaggaa gtttcgaaag attttccttc tttatttcct
      781 tcctttttct ccgcccgctc ttccgtatac caaataccga ttaacataac aatcgcaccg
      841 gaaacaatcg caaaatccgc cagattaaaa accggaaaat tcccgatcca aataaaatcc
      901 acgacatatc ccacgttcgg attgatccgg tccagcagat tgccgatcgc tcctcccagc
      961 tgtagaccga gagcgatttg ccaaaaagcc ggcccgttaa aaacgggttt atagaagaag
     1021 atgattatca aaatcaccac taatccaaac gatgtcaaaa tccatcccat tccctgaaac
     1081 agaccgaaag caacgccggt gtttttccaa tgaataaaac ggaaataagg ttccaaccaa
     1141 gaaatgggca tccaggtttc gcgaataaga agattcgtgc gtaccaacca tttcgttaat
     1201 tgatcaataa aaattacgcc gatcgcaata gaaaataatt tcagataaac ttttccccgt
     1261 ttattcaatc aattcctccg actctcacaa acactaagcc caattatata tcaaaaggga
     1321 ccggccggat ttcggctcag tccctttgtt tatattctcc ctcttacctg tttttacgaa
     1381 aaataatctt ttctttccgg catctaattg atcggaaaat attttcggaa attccccttt
     1441 ttcgcttacg gtaaggagaa ttcataagtg atatcgacgg tcaccacaat ttttaaactg
     1501 ccgctttcca aaggcgccgt accgccgtaa ccaccgccgc ccattccgta catggcaccg
     1561 taatcggtgg gataatcccg aacctgaaaa gatttaatat tgccgactgt cacacctaat
     1621 ttctgcgcaa tctgctgggc attggcattt gcgtcatcga tagccagatc cctcgcttgc
     1681 atatacgtat cttccctcaa agaggaatca taagagacac tgttgatgga gttgacaccg
     1741 ttattgatac aagcatctaa aacatcattc aacttggaaa tatcccgtaa tttgatctca
     1801 aaaccataaa aaacactgaa aatatattca tccattccgg cggtttcatc ccgataatac
     1861 ttttgggtac tgtataacga ataagatgtg gtcttgatat cctcttcggg aatacccatt
     1921 tcagtcaaag ccgactttac cagtttgaca ctcttggtat tttcatcaag cgcctcctga
     1981 acagattctt tttcggtgga gatggataca ttgatgatcg ctatatcggg tttcagcagg
     2041 acggttccgg tcccgctgac cgtcatggtt tgatcggatt gggcctgaac cgaaccggcc
     2101 atacctaacg agagaatcat catactgaga aaaacaatca ttaacaagct gctttttttc
     2161 atttcgactc ctttttattt ctaattttca atttactgtt ctattttgac tcttaatccg
     2221 taagtgcaaa ttccatatcg acactgaccg tcacagtcag aaatcccgaa tcgatcggcg
     2281 ctgtcgatac atcttttacc aatttggtgg aaataagcgg aatttcggta ttatcaagga
     2341 cggaaaaggt ttgcagatga tcaaccttct tgcccaattt ttcggcaatc tgttgggcat
     2401 tcacattcgc gtgatcgatt gccaggtcgc gcgcttgagc aaccgcttca ctccgtttcg
     2461 aataatcaaa atttacccca tataagttgt tcgctccgtt ttggatgcaa aaattcaaaa
     2521 catcattcag catagacaaa tcccgaacga caatttcata tgccaaattt accttataaa
     2581 tatattggcc ggcaatagga ttcccgtctt tatctgtttt cgacaatgaa tacagaatat
     2641 actccgtcgt tcggatatca tcctctttta atcccatttc caatagtcct tgttgaattg
     2701 cgagtgcatt ctttcggttc tcatcgattg ccgttttact gtcgtcggcg gttgtagcga
     2761 tggcgatatc gattaacgcc agatcgggat tcaaggtcac ttctccggta cccttaactt
     2821 tgatgatcgc cgggttttcg ttttgagccg aaactttgaa aacagaaata ccgaacagag
     2881 cgatcgttcc gataaaaagg aacaaaatct ttttaaaatt cattcataag cctgcctttc
     2941 cgtgatccgc aatgggatca atttcgtatt cttaacatgg gatacgtttt ttccgccgtt
     3001 aagttccata gcatctcgct tttattagtc ttttggcatt gaaaatatcg caattcaata
     3061 taattttaat tcggaatgat atatcttggc cggtgatact tcactcacat gaaacagagc
     3121 atttggatcg ggaaatgatg acctgtttca caggaatccg cttgctgccc tgttataatt
     3181 ggcacagaaa tatcttagag gcacgaagcc gaaaagttaa aggggatata tgttggataa
     3241 aatcgatgcg atcgaagctc gttacaacga actcagtaat ttgatcgcag aaaatgtgga
     3301 agattatcaa aaaaccgcag aatggatgaa agaacgttct gaaattgagg agatcgtcga
     3361 aaaaggacgg gaatataaag atacattaaa acggatcgaa gaagacaaag cgttggaaaa
     3421 agatcccgag atgagtgaat tagcatcaac ggaattagac gatctgctt
//
LOCUS       DAOMIV010000191         3449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28901_length_3449_cov_3.928992, whole genome shotgun sequence.
ACCESSION   DAOMIV010000191 DAOMIV010000000
VERSION     DAOMIV010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28901_length_3449_cov_3.928992"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="PKV59_05400"
     CDS             complement(<1..942)
                     /locus_tag="PKV59_05400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQP46223.1"
                     /translation="MDDYRELSQTLSVCPICLEKIPASYTVHPDGHVYLDKDCPSHGH
                     FSTIVWRDNPDFESWTMRSTPYPTEHPSTLIIRGCPYDCGLCPDHQQRPCCVLLEVTR
                     RCNLGCPVCYASSDEYSDLDPSLETIHGWFQMLLTAGGPFNIQLSGGEPTVRDDLPEI
                     IRMGKEMGFGFFQLNTNGIRIAQEKGYLESLSEAGLSVVYLQFDGTEDTIYRKIRGEG
                     LYQTKLKVMERCKEVGIGLVLVPTVIPGVNDENLGEIIRLAVSNLPIVRCVHFQPISY
                     MGRYEKTPENADRITLPELMRKIEEQTNGLIHIENFKP"
     gene            complement(935..1387)
                     /locus_tag="PKV59_05405"
     CDS             complement(935..1387)
                     /locus_tag="PKV59_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HQP46224.1"
                     /translation="MDDSDEIIEMGKQGFTCSQMLMLKALELQGKDNPDLVRSMTGFA
                     GGLGWTGDICGVLTGATAVLSLYAGKGQADDEDDPRLMFMIEDLVHWFHDKYGKIYGG
                     IRCTDILGPDAGKIDMQRCSEIIQDTFQYIKELLVNNGFDLSGESLDG"
     gene            complement(1405..2022)
                     /locus_tag="PKV59_05410"
     CDS             complement(1405..2022)
                     /locus_tag="PKV59_05410"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQP46225.1"
                     /translation="MNPAPGGIALTEKLLNLSLLQKGSMILDVGCGDGDACRFLSSEG
                     YRAIGLDIVRHSSGKMNSERIQGQINAIPILEETVDAVLMECVLSILPTDDGSLTEII
                     RVLKPGGILLFSDLYMRYPDEESEKNRIDSESDYPKIRLADELLNIFGESGLSVRYWE
                     DQSAVLQSLRLKFLWEGSAPENKFPYKLKNIGYICLIAERVSASG"
     gene            complement(2043..2255)
                     /locus_tag="PKV59_05415"
     CDS             complement(2043..2255)
                     /locus_tag="PKV59_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46226.1"
                     /translation="MAQYVNEEDFIHWRCAKCNSELELRKVVVSYLDGSFPVELPVCK
                     VCGQVFIPESLATQKMLEIEQQMEDK"
     gene            complement(2255..>3449)
                     /locus_tag="PKV59_05420"
     CDS             complement(2255..>3449)
                     /locus_tag="PKV59_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HQP46227.1"
                     /translation="IPFQKVVQEARETMVATKKMPPSAFDFALRDMAFSNSEKFRLAK
                     VPVDSKACKYAFFPGCQLPASNPSSIETIFSWLRDEMNEPVGILLRCCGAIADWAGEK
                     EKFNQARSELLADWESLGKPELIVSCPTCLKMFREKYPEINVRSIWPLMAESDSLFNG
                     KSRLNSIKMAIHDPCASRDQPDMQEAVRSLAMKLGIELEELPYNRELATCCSYGGNVW
                     NSNPSLSRDTVEARISQNQSDYLTYCIMCRDFFLRSGKPAYHILDILFDPKLIIEHRL
                     PIRPDYSMRHENRTRVKRHLLKQYWREEMPEEAPYEDIQLILNDPIRKLMEDRMILEE
                     DIRQVLYYAKESGRAMINQETGHYLAHFKPSCVTYWVEYLPKEDGFEIFNVYSHRMFV
                     EEGNI"
BASE COUNT          947 a          826 c          684 g          992 t
ORIGIN      
        1 cggtttaaaa ttttcgatat gaatcaatcc attcgtctgc tcttcgattt tccgcatgag
       61 ttcggggagc gtgatccggt ctgcgttttc gggcgttttt tcataccttc ccatataact
      121 gatcggttga aaatgcacac atcgaacgat agggagattg gaaacggcta agcggataat
      181 ctctcccaaa ttctcatcat tgactcccgg tattaccgta gggaccagaa cgagaccgat
      241 tccgacttct ttacatcgtt ccattacttt taattttgtt tggtataaac cctctccgcg
      301 gattttacga tagatcgtat cctcagtccc gtcaaactgc aaatatacga cagacaatcc
      361 tgcttccgat aatgattcca aataaccttt ttcctgtgcg atacggattc cgttcgtatt
      421 cagctgaaaa aagccgaatc ccatttcttt acccatacga ataatttccg gtaaatcgtc
      481 gcgaactgta ggttctccgc cggaaagttg gatattgaac ggacctcccg ctgtaagaag
      541 catttgaaac cacccgtgta ttgtctccaa agacggatca agatccgaat attcatccga
      601 ggaagcataa caaaccgggc atcccaggtt gcagcgtctg gtcacttcta acaatacaca
      661 acatggcctt tgttggtgat ccgggcagag cccgcaatca tagggacatc cccgaatgat
      721 caaagtggat gggtgttcgg ttggataagg ggtactgcgc atcgtccatg attcgaaatc
      781 cggattatcc cgccaaacga ttgtcgaaaa atgtccatga ctcggacaat ctttatccaa
      841 ataaacatga ccatccggat gaacggtgta acttgcgggg attttctcca agcaaatcgg
      901 gcaaacgctc aaggtttgtg agagttcacg gtaatcatcc atccaaactt tctcccgaga
      961 gatcaaatcc gttgtttacc aatagttctt taatatattg gaaagtgtct tgaattattt
     1021 ccgaacagcg ctgcatatca atttttccgg cgtcgggacc caaaatatcg gtacaacgga
     1081 ttcctccgta aatttttcca tacttgtcat gaaaccagtg aaccaaatct tcaatcataa
     1141 acattaagcg cgggtcatcc tcatcatcgg cttgaccttt tcccgcatat aagctaagaa
     1201 cagcggttgc accggtcaag acaccgcata tatcacccgt ccagcctaat ccaccggcaa
     1261 agcccgtcat agaacggacc aaatccggat tatccttgcc ttggagttcc aaggctttca
     1321 acattaacat ttggctgcaa gtaaaacctt gtttccccat ttctatgatt tcatctgaat
     1381 catccatttt ttctcctttt atcattaccc acttgctgat acacgttcgg ctatcaaaca
     1441 gatatatccg atattcttca atttatatgg gaacttgttt tcgggagcac ttccttccca
     1501 taaaaattta agtcgtaaac tctgcagaac cgcagactga tcttcccagt aacggactga
     1561 aagccctgat tcgccaaaga tattcaataa ttcgtctgcg agacggattt tcggataatc
     1621 gctttcggaa tcgattcgat ttttttctga ttcctcatca gggtatcgca tatagagatc
     1681 actgaacaaa agaattccgc cgggtttcaa cactcgtatt atttccgtta atgaaccatc
     1741 atcggtcggc aagatactca atacgcattc cattaaaacc gcatcgacgg tttcctctaa
     1801 tatgggaata gcgttgattt gtccttgaat tctttccgaa ttcatttttc cggaagaatg
     1861 tcgaacgatg tccaaaccga tcgctcgata tccttccgat gacaggaatc gacaggcatc
     1921 tccgtctccg caaccgacat ctaaaatcat tgagcctttt tggagaaggc tgagattgag
     1981 caacttttcg gtcagggcga ttcctcccgg agcgggattc atccgaaaac agcttcccga
     2041 aattatttat cctccatttg ttgttcaatc tccaacatct tctgtgttgc caaggattcc
     2101 gggatgaaga cctgtccgca gactttacaa accggtaatt caaccggaaa agaaccgtcc
     2161 aaataagaga caacaacttt tctcagttcg agttcactgt tgcattttgc gcagcgccag
     2221 tgaataaaat cttcttcatt gacatattgt gccatcagat atttccctcc tcaacaaaca
     2281 ttcgatgtga atagacattg aaaatttcaa agccgtcttc tttagggagg tattccaccc
     2341 aataggtgac acaggagggt ttaaagtgcg ccaaataatg accggtttcc tgattaatca
     2401 ttgccctccc cgattctttt gcatagtaaa gcacttgacg gatatcttct tcgagaatca
     2461 tccgatcttc catcagttta cggattgggt cattgagaat aagttgtata tcttcataag
     2521 gggcttcttc aggcatttct tccctccaat attgttttaa taaatgtctt tttactcggg
     2581 ttcgattttc atgtcgcatg gaataatcgg gtcgaatcgg taaccgatgt tcgattatta
     2641 atttcggatc gaataaaata tccaaaatat gatatgccgg ttttccggat ctcaaaaaga
     2701 aatcacgaca cattatgcaa taagtcaaat aatccgattg attttgagaa atacgagctt
     2761 caacagtgtc cctgcttaaa gaagggttcg aattccagac gtttccgccg tagctgcaac
     2821 aggttgccaa ctcacgatta tagggcagct cttccaattc aatccctaat ttcatcgcaa
     2881 gggatcggac agcttcttgc atgtccggtt ggtcgcgaga agcgcacggg tcatggatcg
     2941 ccattttaat tgaattcagc cgactttttc cgttgaataa agaatcggat tctgccatta
     3001 atggccagat ggagcggacg tttatctccg gatatttctc gcgaaacatt ttcaaacagg
     3061 tgggacaaga aacaattaat tcgggtttcc ccaacgattc ccaatccgct aacaactccg
     3121 aacgagcttg attaaatttt tctttttcac ctgcccaatc cgcgattgcc ccgcaacagc
     3181 gtaaaagaat gccgaccggt tcattcattt catcccgaag ccatgaaaat atggtttcga
     3241 ttgaggaagg attcgatgcc ggcaattggc agccggggaa aaatgcatat ttgcaagctt
     3301 ttgaatcgac gggaactttc gcgagccgaa atttctcact gtttgaaaaa gccatatctc
     3361 gcagcgcaaa gtcgaaagcg gacggcggca ttttcttcgt tgccaccatt gtttcacgag
     3421 cctcttggac gactttttga aagggaata
//
LOCUS       DAOMIV010000192         3448 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28909_length_3448_cov_5.726201, whole genome shotgun sequence.
ACCESSION   DAOMIV010000192 DAOMIV010000000
VERSION     DAOMIV010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28909_length_3448_cov_5.726201"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PKV59_05425"
     CDS             <1..985
                     /locus_tag="PKV59_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HQP46228.1"
                     /translation="MSEYGTERLLTSIVPSATAAALTSLWTGVSGGTHGLVGYETWMR
                     SLGMVVNFLQYSPSSISSKPGLIGDAGILPEAILPVPTLGNHLMGGGIISRSHMPIAL
                     AHSNLTRAQMAGVSVVPYRRFGDLWENVKELLEADREKVTFDSVYWNDLDTYNHLMGM
                     NDNRIFIDLGSFFDGLARTVQALSRGNFGKTLVLVTADHGHIENTPDPNRDIKNHPEL
                     MHNLMIMPCGENRLTYFYPKSGKDTAIRNYIEETWPDDFYLVSGQELIADGIFGTAAF
                     HPDLDNRIGEWVAIARGYAYFWWPNRPDKLYSRHGGLSDQEMIVPLMGLAL"
     gene            1019..2314
                     /locus_tag="PKV59_05430"
     CDS             1019..2314
                     /locus_tag="PKV59_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQP46229.1"
                     /translation="MTIDRIAVIGQGYVGLPLALSFALHGCHVIGVDSDPDVCSNLKK
                     GITAQTETYQGQSIQTILQTMLQKGTYQITDNGAEAVRRSEVIVLTVGIPIDSGVPVY
                     EYFEAACNTIGENLQKGSLILIRSTVIPGTTENFCKPIFEKKSGMKAGVDFFFAYVPE
                     RIAEGKAFEEFETMPTLIGAPDEKSFDKAAAIIRMISKADIIRSDSVMAVETSKVLEN
                     LQRDANIAIVQEFARFAEAAGMDIMEIIRLANTHKRVNLLTPGPGVGGYCIPNAFHYL
                     NVKADELGIELPLLKLARQQNDRLPQFFADKTEELLQAAGKSIAGAKIAVAGLAMKDF
                     SPDDRLSPAVMICNLLIRSGAKVAAYDKNVATRHPFKVDSFREAANSADALLILNRIS
                     RDFNPLEWIDEMKKPIVILDTRGIIEPEDLPEGTVFWRI"
     gene            2450..3448
                     /gene="hrcA"
                     /locus_tag="PKV59_05435"
                     /pseudo
     CDS             2450..3448
                     /gene="hrcA"
                     /locus_tag="PKV59_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
BASE COUNT          962 a          757 c          868 g          861 t
ORIGIN      
        1 gatgtcggaa tacggaacgg aaagactttt aaccagtatt gtcccgagtg ccactgccgc
       61 agcgttaacc tctctttgga caggcgtttc aggcggcact catggattgg tcggatatga
      121 gacttggatg cgcagcctcg gaatggtcgt caattttctc caatactctc cttcgtcgat
      181 cagctccaaa cccggattaa tcggggatgc cggtattctt ccggaagcca ttcttcccgt
      241 tccgacattg ggaaatcatc tgatgggagg gggaatcatc agccgctctc atatgccgat
      301 tgccctcgcc cattccaatc tgactcgggc gcaaatggcc ggagtttccg tcgttccgta
      361 caggcgattc ggtgatttat gggaaaatgt caaagaactg ttggaagcgg atcgcgaaaa
      421 agttactttt gactccgttt attggaatga tctcgatacg tataatcatt tgatggggat
      481 gaacgataat cgaattttca tcgacctggg atcctttttc gatggtctgg cgagaacggt
      541 tcaagctctg tcccgaggaa acttcggaaa gacattagtt ctggtcaccg cagatcacgg
      601 ccatatcgaa aatacaccgg atcctaaccg ggatatcaaa aatcatccgg aattgatgca
      661 taatttgatg attatgcctt gtggagaaaa ccggctgacc tatttttatc cgaaatccgg
      721 caaagatacg gcgattcgta attatatcga agaaacttgg ccggatgact tctacttggt
      781 tagcgggcaa gagctgattg cggacgggat attcggaacc gctgctttcc atcccgatct
      841 ggataatcgg atcggtgaat gggtcgcaat agcccgcgga tacgcctatt tttggtggcc
      901 gaatcgtccg gataaactct attcgagaca cggcggtctt tcggatcagg agatgatcgt
      961 cccgctgatg ggtttggcgc tctaaatgaa ttcaagtctt taaaggatgt gaaaacttat
     1021 gacaattgac aggattgctg ttatcggaca aggatatgtg gggcttcctt tggcactcag
     1081 ttttgctctt cacgggtgtc atgtcattgg cgtcgatagc gaccctgatg tttgttccaa
     1141 tctgaaaaaa ggaatcaccg cacaaacgga aacctatcag ggccaatcga ttcaaactat
     1201 tcttcagacc atgctgcaaa aaggaacata tcagatcacc gacaacggag ccgaggctgt
     1261 caggcgatcg gaggtaatcg ttttaacggt cgggataccg atcgattcgg gtgttcctgt
     1321 ttacgagtac tttgaagccg cctgcaacac gatcggagaa aaccttcaaa aaggcagtct
     1381 gatccttatt cgcagcacag ttattcccgg taccaccgag aatttttgca agccgatatt
     1441 tgagaaaaaa tccggaatga aagccggggt tgatttcttt tttgcttatg ttcccgaacg
     1501 aattgcggaa gggaaagctt ttgaagaatt tgagacgatg ccgactctga tcggcgctcc
     1561 ggatgaaaaa agttttgata aagctgcagc aattatccgc atgatcagta aagccgatat
     1621 catccgctcc gattccgtca tggcggtaga aacatcaaaa gtactggaaa atctgcagag
     1681 ggacgcaaat atagccatag ttcaggaatt tgcccgattt gccgaagcgg ccggaatgga
     1741 tatcatggaa atcatccgat tagccaatac acacaagcgg gtcaatttgt tgactcccgg
     1801 acccggggta ggcggttact gcattccgaa tgccttccac tatctcaacg tcaaagccga
     1861 tgaattggga atcgaattgc ctttgttgaa attagcgaga cagcaaaatg atcgcttacc
     1921 gcaatttttc gcggataaaa ccgaagaact cttgcaagct gccggcaaat cgatcgctgg
     1981 ggcaaaaatc gcggtagcgg gactcgccat gaaagatttc tcaccggacg atcggctgag
     2041 tccggctgta atgatctgca atttactgat ccgatccgga gcaaaggtag ccgcttatga
     2101 caaaaacgtc gccactcgcc atccttttaa agtcgattcc tttagagaag ctgcgaattc
     2161 cgcggatgcg cttctcattc tcaacaggat ctcgagagat tttaatccgc ttgaatggat
     2221 cgatgagatg aaaaaaccga tagttatctt ggataccaga ggaataatcg aacccgaaga
     2281 tctgcccgag gggaccgttt tctggagaat ttgattcgaa atcccttttt aaatatttgt
     2341 taacctttcg ttagaaaact tcataaatgc ttgacggtca ttttttggcg tggtattata
     2401 ctgtaggttt gattagcact ctaatcgcaa gagtgctaac ggggcgaaaa tggaaacttt
     2461 gacggaaagg caaaaattaa ttctcagttt ggtagtccat gactacacaa aaaccgcgag
     2521 acccgtgggt tctaagagac ttgtcgaaaa attcaggtta gatttcagcc ccgccacagt
     2581 ccgaaacgaa ttggctgctc tgacggaaat gaacttttta cgtcaaccgc acacctcagc
     2641 cggaagagtg ccaaccgaaa aagggtaccg atattttgtc ggaacactga tgcaaacaca
     2701 agaattatcc gaagagaatc gaaggatgat cgaacatcag tttttccaaa cccagcagga
     2761 acagggaata gacggatgga tgaaactggc ggcatcgatt ctcgcggtgc agtccaatgc
     2821 gatttcggtc gtctctgccc cgatgccggt tcggaacgga ttgaagcacg ttgccgtgat
     2881 cggaatcaca gggaagcaga tcctgttagt gctcgttttg gacggcggcc aaatgcaaca
     2941 gcgattcata acgcttgatg agcaaacatc gcaaaatgaa ttgactgtga ttgcgaatga
     3001 aattaatcag cgatttaaag gcatgactac cgagcaaatt cgcaaggaaa gtcaaattga
     3061 gaagtcaaga gaccctttaa tggaactgat tttgaaaaac gtattaaatg cgatgtacga
     3121 aacagatgca atgaaagccg gagaagtcta taccgacggt atcagtaacg tcttagccga
     3181 accggaattt tcggaatcgg aagatgcgcg ccgagcgttg aaaattctgg aagaaagacc
     3241 tgccctgcag agctttcttt cgagaacgac atgggatcag aagatcggcg gtgttcaggt
     3301 actgatcggc ggggaagggt tgttggcaaa tttgagaaac tgttcgatgg ttttagcgtg
     3361 atatggtcta ccggacacat tgaccggaac ggtaggtgtg attggaccaa tgcgtatgcc
     3421 atacagccga aacatcccga cagttcga
//
LOCUS       DAOMIV010000193         3442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28988_length_3442_cov_3.814290, whole genome shotgun sequence.
ACCESSION   DAOMIV010000193 DAOMIV010000000
VERSION     DAOMIV010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28988_length_3442_cov_3.814290"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1282
                     /locus_tag="PKV59_05440"
     CDS             89..1282
                     /locus_tag="PKV59_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HQP46230.1"
                     /translation="MYRLSNTQKEVVLQWQRFFHRNPEISFNEVKTSEKITEILNHYG
                     IENHRVGQSTGISAEITGELPSDSPKRIMLRADIDALPMEEQNDLPWKSQNPGAAHTC
                     GHDGHTAALLGAAVLLNDDRASFSGSVRLIFQPAEESVGGASIMIREGELDGVDLAAG
                     IHFWPALQSGKIACEYGAAMAGCEHFCIRFFGKGGHISVPHLANNPILSASETFIQLQ
                     TAAAQQFSPFEELLIGVGKIVGGTQYNIIPEEALLEGTIRAFQPETLQKVRQVVRNTA
                     EHIGAANNNTCDISFGNGIPPVMNDPKAVDLAIQTASALIGKENVLTEIPKQFTGDDF
                     SFYAVEKPAAYIKIGSWDPSRPETNNPLHSPRFNLDEKALYLAAEYFANYACAFLRNP
                     AEESI"
     gene            1586..3178
                     /locus_tag="PKV59_05445"
     CDS             1586..3178
                     /locus_tag="PKV59_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46231.1"
                     /translation="MNKNFFLVFCLMLTAILFSTVGAFAQSESNSLTVALSVEPVSLD
                     PAGGLYIPEQLLIQQIYDPLIYADPEQNLHPGLATSWEVNPEGTEFTLNLRNDVKFHD
                     GTPFNAQAVKVSLDRAAVGTTVAAAAPAILTDYIETQVVNDTQVVIKFSTPHATFLQD
                     LTRPWLMISSPAAIEQYGDDYGQHPVGTGPFIFKEWAAQDHITLVKNPDYNWGPEFFT
                     HTGPALLDEIVFRVLPEAAIRLTAFQTGEALLVQDPSYLEASQMANDGSTQVFTFTAP
                     GMTSHQMINVEKAPTDDINVRKAMIYAVDQETIVQTAFYGLQSAAHSVISPTTWAFNE
                     EANNLYRYDPKKAAELLEESGWVDTNGDGIREKNGVNLHIEYPALPAYEEAFMELLAY
                     YLNQAGFEVNITKLDDAGISEFGYAGKHNILNMGWISRDPSVLSYVYLSDNIEGGNQS
                     AYSRFRNARLDEILKTAPQTIDADQRKALYEEAQMIIMENAIALPIHTYGNVYLAAKN
                     VKGFRFDPEGYPYFYEISLAAE"
     gene            3182..>3442
                     /locus_tag="PKV59_05450"
     CDS             3182..>3442
                     /locus_tag="PKV59_05450"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46232.1"
                     /translation="MGSYLGRRLLTSLSILIGVSILTFLMLQFVPGDPVRALIGRQTV
                     TPERLEEIKQEMGLNDPIPVQYFNYLKKVLSGDFGRSLKNNRP"
BASE COUNT          929 a          792 c          802 g          919 t
ORIGIN      
        1 attatcataa tcatttctta ataagctgcg ttgagttgaa taattctatt tataatgaca
       61 gaaacgtcat ttgaaaggag ttgaaacttt gtaccgttta tcaaacactc aaaaagaagt
      121 tgtgctgcaa tggcaacgtt ttttccatcg aaatcccgaa attagtttta atgaagtgaa
      181 gacttccgaa aaaatcaccg aaatcctgaa tcattatggg atcgaaaatc acagagtggg
      241 ccaatcgacc ggaatctccg ctgaaatcac cggcgagctg ccatccgatt caccgaaacg
      301 gatcatgtta cgcgccgaca tcgatgcatt gcccatggaa gaacaaaatg atctcccctg
      361 gaaatctcaa aaccccggtg cggcacatac ctgcggtcat gatggtcata ccgccgctct
      421 tttaggagcc gctgttttac taaacgatga ccgcgcgtct ttttccggtt ccgttcgttt
      481 gatattccag ccggcggaag aatcggtcgg cggggcgagc atcatgattc gggaagggga
      541 attagacggc gtcgacttgg cagccggcat tcacttctgg ccggcgttgc aaagcggaaa
      601 aattgcctgt gaatacggag cagctatggc gggatgcgaa catttttgca ttcgcttttt
      661 cggaaaaggt ggccatatca gtgtaccgca tctcgcaaac aatccgatcc tttcggcttc
      721 ggagacattt atccagctgc agaccgcagc tgcccaacaa ttttcgccgt tcgaagagtt
      781 actgatcgga gtcggaaaaa tcgtcggcgg aacccagtac aacatcattc cggaagaagc
      841 gcttttggaa ggcacaatca gagcttttca acccgagaca ctacaaaaag tccgtcaggt
      901 cgttcgaaat acggccgaac atattggggc agcaaacaat aacacctgtg atatttcttt
      961 cggcaatgga attccaccgg tcatgaatga cccgaaagcc gtggatttgg cgattcagac
     1021 cgcttcggcg ctgatcggga aagagaatgt tttgacagaa atacccaaac aatttaccgg
     1081 agatgatttt tctttttatg cagtcgagaa gccggccgca tatatcaaaa tcggctcttg
     1141 ggacccgtcg aggccggaaa cgaataatcc gttacacagc ccgcgtttca atctggatga
     1201 gaaagcgctt tacttggctg cggaatattt tgcgaattac gcctgcgctt ttttgcggaa
     1261 tccggctgaa gaatcgattt agatcctcaa ttcgtgccct tcaagatcgg ataaaaaact
     1321 tgtaatattt ttcgtgatga ttcggtcatt gcttttataa tttttgccat gaataaaatg
     1381 aaacttttaa ccgatagcaa actttgcttg tgtttggggc gaggaatact gttcataacc
     1441 agttttggct gaatttgctg atattcagaa attggcgaac ggaattcctc gctttttaca
     1501 gttgagacat ccgttcgttt tttatttaaa cggtttctga ccgttcggat gtcaacagaa
     1561 aggttttatt tgaaaaggag ctgcaatgaa taaaaatttt tttcttgtct tttgccttat
     1621 gcttacggcg atcctttttt cgacggtcgg agctttcgct caaagtgaaa gcaacagcct
     1681 gactgtcgct ctttcggttg agcccgtttc tctggatccg gcgggcggat tgtatattcc
     1741 ggaacaattg ctgattcaac aaatctatga tccgctgatt tatgcggacc ctgaacaaaa
     1801 tcttcatccg gggctggcta cttcttggga agttaacccg gaaggaacag aatttacttt
     1861 aaaccttcgc aatgatgtga aatttcatga cggaacccct ttcaacgccc aagcggtaaa
     1921 agtttctctg gatcgcgctg cggtcggaac cactgtggca gcggccgcac cggctattct
     1981 tacggactat attgaaacgc aagtggttaa cgatacccaa gtggttatca aattctcgac
     2041 tccgcatgcg acctttctgc aggatttgac gcgcccgtgg ctgatgattt cttccccggc
     2101 cgccatcgaa caatacgggg atgattacgg acaacatccg gtcggaaccg gccccttcat
     2161 tttcaaagag tgggcagcac aggatcatat cacgcttgtc aagaatcccg attacaattg
     2221 gggcccggaa ttcttcactc ataccggacc tgccttgttg gacgaaatcg tatttcgggt
     2281 tttgccggaa gcggcaattc gtctgaccgc atttcaaacc ggtgaagcac tgctggttca
     2341 ggacccgtcc tatctggaag cttcgcagat ggcgaatgac ggttccaccc aagttttcac
     2401 tttcaccgct cccggaatga catctcatca gatgatcaat gtcgaaaaag cgccgaccga
     2461 tgacattaat gttcgaaaag caatgattta cgccgtcgac caggaaacaa tcgttcaaac
     2521 cgctttttat ggactgcaga gtgcggctca cagcgtcatc tcacccacta cctgggcatt
     2581 taacgaggaa gcgaataatc tctatcgtta tgacccgaaa aaagcagccg aactccttga
     2641 agaatcgggc tgggtcgata caaacgggga tggaattcgc gaaaagaatg gcgttaacct
     2701 tcatatcgaa tacccggcat tgcccgctta tgaagaggca tttatggagt tgctcgcata
     2761 ctatctgaat caggctgggt ttgaagtgaa tattaccaag ctggacgatg cgggtatttc
     2821 cgaattcggt tatgccggta aacataatat tctgaatatg ggttggatca gccgcgatcc
     2881 gtctgtcctc agctatgtct atctctccga taatattgaa ggcggaaatc aatccgctta
     2941 ctcgagattc cggaatgcac ggttagacga aatcctcaag acggctccgc agacaatcga
     3001 tgccgatcaa cgaaaagcac tttatgaaga agcacaaatg attattatgg aaaacgcaat
     3061 cgctctgccg atccatacct acgggaatgt ttatttggcg gcaaagaatg taaaaggctt
     3121 ccgttttgat ccggaaggtt acccatactt ttacgagatt tctctcgcag ctgagtaaag
     3181 gatgggaagt tatttaggtc ggaggttatt gacctccctg tcaatcctga tcggggtgtc
     3241 aattttaact tttctgatgt tacagtttgt ccctggtgac ccggttcggg ctttaatcgg
     3301 aagacaaaca gttacacccg aaagacttga agaaatcaaa caggaaatgg gattaaacga
     3361 tcccattcct gttcaatatt ttaattattt aaaaaaagta ttatccgggg atttcggccg
     3421 ctcgctgaaa aataatcgac cg
//
LOCUS       DAOMIV010000194         3441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_28998_length_3441_cov_3.681926, whole genome shotgun sequence.
ACCESSION   DAOMIV010000194 DAOMIV010000000
VERSION     DAOMIV010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28998_length_3441_cov_3.681926"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="PKV59_05455"
     CDS             <1..895
                     /locus_tag="PKV59_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQP46233.1"
                     /translation="TGRVSGFNFAERAMWEAGDNVIFQVPAYPPFFEAPINNNLGSVL
                     NPLVFNKDKNIYEIDFDDFEKKINPRTKMLLLCNPQNPTGRVFTPAELTRLGEICLKH
                     DLIICSDEIHSDIVYNGYRHTPIASLNKELENRSITFIAPSKTFNIPGLSCSVAIIPN
                     PEIHKKYLKALNGFCSGVTCLSQEAGIAAYQEGAEWLSQMKIYLEGNRDFLLNFISRE
                     MPSIKTTEIQATFLAWLDCSQTGIPGDPQKFFEENAKVGLNEGIPFGPDYEKFVRLNF
                     GTPRKILKEALERMANSLRQL"
     gene            915..1586
                     /locus_tag="PKV59_05460"
     CDS             915..1586
                     /locus_tag="PKV59_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HQP46234.1"
                     /translation="MGSSETESNQPVIQRKKNIRKWIILLIIIAVLLGFLSLAGEYLT
                     IDSELNHADLIVVLSGNDTVRLKAAAELYKEGYSETILLTNTGRTYGEYNIPYTQLQI
                     EMLKELDVPEGAIHQVDFVAKNTGQEGTAIIEGMVNLRANSVIIVTDAWHTRRVKTIF
                     DDTFSNTNYSVQYYGVSESEINPKLWWLSADGWKNVIGEYIRIIGYYIKRDTNIPDYP
                     IFNIS"
     gene            complement(1643..1858)
                     /locus_tag="PKV59_05465"
     CDS             complement(1643..1858)
                     /locus_tag="PKV59_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQP46235.1"
                     /translation="MSERYVGTVKWFNATKGYGFIGRDGDSPDVFVHFSEIKMDGYKR
                     LNEGQKVEFSIEEGSRGAQATDVKVIE"
     gene            2155..2649
                     /locus_tag="PKV59_05470"
     CDS             2155..2649
                     /locus_tag="PKV59_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQP46236.1"
                     /translation="MTEKNNRPAKRINWKVVISLTAIIGGVLYLLISSTINNAQYFIT
                     VDELLANRQQYANKSVRVSGAVIGDTIKVSETDGSVSFEVANISADHKAIEENGGMAK
                     ALAEAVNDPSAAHLKVLYTGPKPDLLKDQAQAILTGTINNNNVFMADELLLKCPTKYE
                     GAMQ"
     gene            2651..>3441
                     /gene="ccsA"
                     /locus_tag="PKV59_05475"
     CDS             2651..>3441
                     /gene="ccsA"
                     /locus_tag="PKV59_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQP46237.1"
                     /translation="MATLGYSSLVFALFAEIYGVAALMIGSVRKSKKWLSSGRQACLI
                     LFPLLSISVIALLIPLIRKDYRFEYVFETVNNAQPFYLRIAALWGKQSGSLLFWTWLF
                     SIFLFVWTLKSVKKDDPLFPTAAILLLLNIGFFNVLNLFGSNPFNRFWLNNLNGKVYT
                     AFSQISGTVSYQPSDGMGMNPLLRHFGMVLHPPALYLGFIGVFIPAAYELSALIHKPD
                     HFDWIRKTHSWFLFAWIFLAIGLILGSRWAYDVLGWGGYWGWDPV"
BASE COUNT          973 a          714 c          722 g         1032 t
ORIGIN      
        1 aacgggaagg gtatccggat ttaactttgc cgaaagagct atgtgggaag ccggagataa
       61 tgttatcttt caggtccctg catacccgcc tttttttgag gctcccataa acaacaatct
      121 gggaagtgtc ttgaatcctt tggtcttcaa caaggataag aatatctacg aaatcgattt
      181 cgatgatttc gagaaaaaaa tcaatccgcg aacaaagatg ctgcttcttt gcaatccgca
      241 aaatccgacg ggacgggttt ttacgccagc tgagctgacc cgattgggag aaatttgcct
      301 gaaacatgac ttgatcatct gctcggatga gattcacagt gatattgttt acaacggtta
      361 ccgtcatact ccgatcgctt ccctgaacaa agaactcgag aatcgttcca taacctttat
      421 cgcccccagc aaaacattta atattccggg gctctcttgt tcggtcgcga ttattccgaa
      481 tccggaaatt cataagaaat atctcaaagc actcaacggt ttttgcagcg gggtgacctg
      541 tctttcacag gaagccggaa tagcggctta tcaggaaggt gcggaatggc tctctcaaat
      601 gaaaatatat ttggaaggaa accgtgattt tttgttgaat ttcatcagcc gggaaatgcc
      661 gtctataaaa acaaccgaaa ttcaagcaac ttttttggct tggttggatt gttcgcagac
      721 aggtattccc ggagatcctc agaaattctt cgaggaaaat gctaaagtcg gtttaaatga
      781 aggcattccg tttggtcccg actacgaaaa attcgtccgg ttgaatttcg gaacaccgcg
      841 gaaaattctg aaagaagcgc tggagagaat ggcgaattcg cttcggcaac tttaatcaac
      901 acgatcaaat aagtatgggt tcgtcagaga ccgagagcaa tcaacctgtc attcaaagga
      961 aaaagaatat ccgaaaatgg ataatccttc tgattattat tgccgttttg ttgggttttc
     1021 tttcgcttgc cggtgaatac ttgacgattg attcggaatt gaaccatgcc gatttgattg
     1081 tcgtattaag tggaaatgat accgttcggt taaaagccgc tgccgaactt tataaagaag
     1141 ggtattccga aaccatttta ttaaccaata ccggtcgtac ttacggagaa tataatattc
     1201 cttatacaca attgcagatc gagatgttga aagagctgga tgttccggag ggtgcgattc
     1261 atcaagtcga ttttgtcgca aaaaacaccg ggcaggaagg gaccgccatt atcgaaggaa
     1321 tggttaatct gcgtgcgaat tcggttatta ttgtcacgga tgcatggcat actcgtcgcg
     1381 taaaaacgat ttttgacgat actttttcca acacaaatta ttccgtccaa tattacggag
     1441 tgtcggagtc cgaaataaat ccgaaattat ggtggctttc tgcggacgga tggaaaaatg
     1501 tcatcggaga atatattcga atcatcggct attacatcaa aagagatacg aatattccgg
     1561 attaccccat ttttaatatt tcttgattct aaaaaaaaga gactgctcat ttccggagca
     1621 gtctctaaaa ttttccttta tcttactcga tcaccttcac atcggttgcc tgagcgccgc
     1681 gcgaaccttc ttcaattgaa aattcaactt tttgtccttc attaagccgt ttatatccgt
     1741 ccatcttgat ttccgagaaa tgaacgaaaa cgtccggaga atctccgtct cgaccgataa
     1801 aaccatagcc ttttgtagca ttaaaccact ttactgtacc tacatatctt tcactcattt
     1861 tattaactcc gattgttctc ttaattccat ttcggcaacc cctctgcctc atgttttatc
     1921 gtgctgaaaa gaaaattttg agggttcttt tcaaacgtta atatttttat cactcataaa
     1981 aaaaagcgtc aaggaaaagc atcgaaaatt tttgaatttt tacctgtagg ttttatagtt
     2041 gactccgaaa atctgcataa tctcccttta atcgggaaag agaaatactc aagaatgata
     2101 aaattatgtc tgtttaataa agtcttcaaa aatgccttct tttcggggga agaaatgaca
     2161 gaaaaaaata atcgcccggc aaaaagaatt aactggaaag ttgtaatcag tctgaccgca
     2221 atcatcggcg gagttcttta cctgcttatt tcctcaacaa tcaataacgc gcaatatttc
     2281 attaccgtag atgaacttct tgcaaatcgc caacagtatg cgaataagtc ggttcgagtt
     2341 tcgggcgcgg ttatcggaga caccatcaaa gtctcggaga cggatggatc cgtttcattt
     2401 gaagtggcaa atatttctgc cgaccataaa gcaatcgaag aaaacggcgg aatggcaaag
     2461 gcattagccg aagcggttaa tgatccttcc gccgctcatc tgaaagttct ttacaccggt
     2521 ccgaagcctg atttgctaaa agatcaagcc caagccatcc tgaccggaac aatcaacaac
     2581 aataatgtat ttatggcgga tgagctttta ttaaaatgcc cgacaaaata cgaaggagcg
     2641 atgcaataag atggcaacct taggatattc ttcattagtc tttgccctgt ttgctgaaat
     2701 ctacggagtc gcggcgctga tgatcggatc cgttcggaaa tcaaaaaaat ggctgagcag
     2761 cggtcgtcaa gcttgtttga tcctttttcc gctgctcagt atttcggtta tcgctttatt
     2821 gattcctttg attcggaaag attatcgttt cgaatatgta tttgaaacgg ttaataatgc
     2881 ccaacctttt tacctccgga tcgcggcttt atgggggaaa caatccggat cgctcctttt
     2941 ttggacctgg cttttttcaa ttttcttgtt cgtttggaca ttaaaaagcg tcaagaagga
     3001 tgatccgcta ttcccgacag ctgctattct gctgctctta aatatcggat tcttcaatgt
     3061 tttaaatctc ttcgggagca acccttttaa tcgtttttgg ttaaacaatt taaacgggaa
     3121 agtttatacc gctttttctc agatttccgg taccgtttct tatcaaccct ccgatggtat
     3181 gggaatgaat cccttattac ggcatttcgg tatggttctt catccgccgg cactttattt
     3241 gggatttatc ggggttttca ttccggcagc ctatgaatta agcgctttaa ttcacaaacc
     3301 cgatcatttt gattggatcc gaaaaactca ctcatggttt ctgttcgcat ggatttttct
     3361 ggcaatcgga ttgatcttag gatctcgatg ggcgtatgat gttctcggtt ggggcggata
     3421 ttggggttgg gatccggtcg a
//
LOCUS       DAOMIV010000195         3431 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_29105_length_3431_cov_3.349526, whole genome shotgun sequence.
ACCESSION   DAOMIV010000195 DAOMIV010000000
VERSION     DAOMIV010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29105_length_3431_cov_3.349526"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /gene="wecB"
                     /locus_tag="PKV59_05480"
     CDS             <1..682
                     /gene="wecB"
                     /locus_tag="PKV59_05480"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQP46238.1"
                     /translation="HFAPTEMARQNLLREGIPPEICHVTGNTVIDAQQLALQIPFEEK
                     GTPLEGIPFGEKEIITVTAHRRENHGKPLVDICNAILKISQVYADRVHIVYPVHLNPI
                     VRKTAFSILDHQPNISLIPPLDYLSMVYLLKNSKILLTDSGGLQEEAPGMGVPVLVLR
                     EVTERPEGVISGNVRLVGTDPENIYTHVCDLLDHPDQWEKMSKATNPYGDGKAAERIV
                     KLINAEQK"
     gene            1003..1125
                     /locus_tag="PKV59_05485"
     CDS             1003..1125
                     /locus_tag="PKV59_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46239.1"
                     /translation="MADTGKNDKAKKEQKKKPKMTIKEKRKLKREKTNKETGAV"
     gene            1684..3351
                     /locus_tag="PKV59_05490"
     CDS             1684..3351
                     /locus_tag="PKV59_05490"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQP46240.1"
                     /translation="MNKKVIIVGGVAGGATTAARLRRLDENAEIILFERGKYISYANC
                     GLPYHVGDVIPSREDLLLQTPESLKSRFNIDVRIQQEVTFIDRANRTVSVKNLATSQE
                     SVESYDTLVLATGSSPIIPNIPGIRSKYIFSLWTVDDADHIRQYIRQNKISDAIIIGG
                     GFIGIEMTENLRRLGITVSLVEMLNQVMAPFDFEMAQLIHKTLEENGVSLYLGNGVTS
                     FTETDESILVNLSDGKQLATRLVILSIGVKPNSELARSAGLALNARGGIVVDKTLRTS
                     DPDIFAVGDVIEVEDFISKGKTMIPLAGPANKQGRIAAGNIAGENDQYPGTQGSAIVK
                     IFDVCAANTGANEKTLLKNGLIKGKDYEKLIILQNSHAGYYPGASPIAIKLLFSTDGN
                     KIFGAQIIGKEGVDKRIDTIGTAIRLGGTIWDLKSLEFAYAPPFSSAKDPVNMAGFVA
                     ENILKSRVFFAEWDVVERHDPEKTILLDVREDAERRTYALSGALNIPLGQIRSRLSEI
                     DKEKEIIIFCAMGVRAYNAARILMANGFQNVKVYPGGMRFFRATHYK"
BASE COUNT         1096 a          646 c          741 g          948 t
ORIGIN      
        1 gcacttcgcc cctactgaaa tggcacgcca aaatctgctc cgtgagggaa tacctccgga
       61 aatctgccat gttaccggaa atacggtaat tgacgctcag caattggcat tacaaatccc
      121 gtttgaagaa aaaggtactc ctttggaagg gatccctttc ggagaaaaag aaattataac
      181 cgtcacggca catcgaaggg aaaaccatgg aaagccgtta gtcgatatat gtaatgcaat
      241 cttaaaaatc agtcaagttt atgcagatcg tgttcatatt gtttacccgg tccacctgaa
      301 tccgatcgtc cgcaaaacgg ctttttcgat tttggatcat caaccgaata tcagcttgat
      361 tccgcctttg gattatcttt cgatggtgta tctattgaaa aacagcaaga tattactcac
      421 tgattcggga gggttacagg aagaggctcc ggggatgggt gtgccggtat tggtacttcg
      481 ggaagtgacc gaacgaccgg aaggcgtgat ctcgggaaat gtacgattag tggggactga
      541 tcccgaaaat atttatacgc acgtatgcga tctgctggat caccccgatc aatgggaaaa
      601 aatgtccaaa gccacaaacc cttatggtga cggaaaagcc gcggaaagaa ttgtcaagtt
      661 gattaatgcc gaacaaaaat aaatgttgaa aggtctgttt ttttcgatat aatttattaa
      721 caaacaaatt caggaaaatc tgagaatcgg aagaagctct gattgtttaa ccgtcagata
      781 cttgctgaga gaaaggagcc aaaatgctta atcattttct tcggtttgct tcacttatac
      841 tggtccgatt cctataaaaa atattatagg ccggattaaa tgaccgagga aatatacatc
      901 tggtttgttg acaagtaaat caatttcaaa tagtatagaa aaaatgattg aatgatattc
      961 atttcttcgt aaaccggttc aagaaaaaca taggaggttg ttatggctga tacaggaaaa
     1021 aatgataagg ctaaaaaaga gcaaaaaaag aagccgaaaa tgacaattaa agaaaaaaga
     1081 aagttaaaac gggagaaaac aaataaggaa acaggagctg tctaaagacg gaatcgaaac
     1141 ttctttaaat tgattttaaa caatatatga tggataaacc cgaaaatcag aaagaaaagt
     1201 gtgcgtaatt ttgcagcacg cttttcttta aatctaaaaa acggaattca ttccgaatgg
     1261 attcctattc attaaattta caataaatga tcggcagatc agatttatga gacagaaatg
     1321 aaatatatcc ttcctcaaaa gttcaatttt tttattccat agaccgtttt tataattttt
     1381 ttcataaggc cgatcaaatt tcatttaatt tattaaaaag cacttcgttg aagcattttc
     1441 atcgcaaggg agaatttcgt ttcaaagagt ttgccgctct tataaaattc ggcaattttt
     1501 ttttataaaa gtcgcggtga atatatgaac tttatcgatc gattttcata aagtccaatg
     1561 atcaatcttt tggaccgccc atttatataa tccgaaatat ggaaaaatat cgtaagttgt
     1621 ttgcagatat gttaagataa atgaaaatcg gatttcaaaa tccttaatca ggaggcaact
     1681 ttcatgaaca agaaagtcat cattgtaggc ggtgttgcgg ggggcgctac aaccgcagcc
     1741 aggttgcgca gactggacga aaatgcggag atcatccttt tcgaacgcgg gaaatatatt
     1801 tcttatgcca attgcggttt accttatcat gtgggagatg taatcccatc gcgggaggac
     1861 cttctgttgc agaccccgga atctttgaaa tccagattca acatagatgt tcgtattcag
     1921 caggaagtaa cttttattga tcgtgcaaac agaactgtct ctgttaaaaa tttagccact
     1981 tctcaagaat cggttgaaag ttatgataca ttggtcctgg ctacggggtc ttcaccgata
     2041 ataccgaata tcccgggtat ccgatcaaag tatatttttt cactctggac cgttgatgat
     2101 gcggatcaca tccgtcaata tatccggcaa aataaaatca gcgatgccat aataatcgga
     2161 ggtgggttca tcggcatcga aatgaccgaa aacctgcgcc ggcttggaat tactgtatct
     2221 ttggttgaaa tgttaaacca ggtaatggct ccgtttgatt ttgaaatggc acagttaatt
     2281 cataaaacat tggaagaaaa tggtgtgtcg ctttatttgg gaaacggcgt cacctctttt
     2341 acggaaactg atgaatccat tctggttaat ctatccgatg gcaagcaatt agctacccga
     2401 ctcgtaattt tatccatcgg cgtcaaacca aacagcgagc ttgctcgttc agcgggctta
     2461 gcgttaaatg cccgcggcgg aattgttgtt gataaaacac tacgaacatc cgatcccgac
     2521 atctttgcgg tcggcgatgt gattgaagtc gaagatttta tcagtaaagg caaaactatg
     2581 attccgttag ccggaccggc taacaaacag ggacgcatcg ctgccggtaa catcgccgga
     2641 gaaaacgatc aatacccggg aacccaagga tcggcgattg tcaagatctt tgatgtatgt
     2701 gccgcaaata cgggcgcgaa tgaaaaaaca ttattaaaaa atggcctgat caaaggaaag
     2761 gattatgaaa aattaatcat tctgcaaaat tctcatgccg gatactatcc gggtgcctcc
     2821 ccgatagcta ttaaactgct tttttcaaca gacggaaata aaatcttcgg agcccagatt
     2881 atcgggaaag aaggagtgga taaacggatc gataccatcg gcaccgctat tcgtctcggc
     2941 ggaacaattt gggatctgaa atcacttgaa ttcgcttatg cacctccttt ttcttctgcg
     3001 aaagacccgg tcaatatggc aggatttgtc gcggaaaata ttctcaaatc cagagtcttt
     3061 tttgccgaat gggatgttgt ggaacgtcat gatccggaaa aaaccatatt gttggatgtg
     3121 cgcgaagatg cagaacgacg aacttatgct ttgtcggggg ctttgaatat tccattggga
     3181 cagatccgat caagactgtc tgaaattgac aaagagaaag aaatcattat tttttgtgca
     3241 atgggtgtcc gtgcttataa cgctgcaaga atcctaatgg ctaacggttt tcaaaatgtg
     3301 aaagtttatc cgggcggcat gaggtttttc cgcgcaaccc attataaata gagccggtaa
     3361 gatcattgtt cagaatgatc atcaaatttc gaacaatgat ctcagattaa tcggttccga
     3421 caacggatgt t
//
LOCUS       DAOMIV010000196         3397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_29536_length_3397_cov_4.166966, whole genome shotgun sequence.
ACCESSION   DAOMIV010000196 DAOMIV010000000
VERSION     DAOMIV010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29536_length_3397_cov_4.166966"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..1105)
                     /locus_tag="PKV59_05495"
     CDS             complement(152..1105)
                     /locus_tag="PKV59_05495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQP46241.1"
                     /translation="MDQPEISVIIPFLNEEENLPDLIAELEAFAPTMPVRMEVVFVDD
                     GSTDGSLDLLRKVHFRNFSAKIVKLSRNFGSHPAVRAGFLNAAAPNCVWLGADLQEPL
                     EIIRLGYQKIREGYDLVCVQKKNVKVGKSEKLFSNTYSRLIRKYAIPGYPPDGVNTVF
                     FNEKVKRELNRNIESNSSVVLQVLNLGFKQTTLDVEYRERVHGRSKWSFGKKMKLMID
                     SFVAFSFFPIRMVSAMGFIFAIIGAIIALYLIIIKLFNIREVTLGWPSLISILMIGFG
                     ITNISLGVIAEYLWRTLDAARGRPSFIIESVEGLSQANQND"
     assembly_gap    1435..1534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1535..2165)
                     /locus_tag="PKV59_05500"
     CDS             complement(<1535..2165)
                     /locus_tag="PKV59_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQP46242.1"
                     /translation="MKSILRKSILYKSGISSLDYAMNPVLGCAHGCRYPCYAWLMAMR
                     FGKVKSYSQWIQPAIVGNTLELLEKELRKWHWKIHHVQLCLTTDPFMVGYPEVRQLSL
                     ASIRMINQNGIPCGILTKGVLPPELAEMDQRNSYGISLVSLDEEFRERCEPGAAPYRE
                     RIDALRYLHNKGMHTWVHMEPYPTPNVISQDLNEILEEISFVDSIDFGRW"
     gene            complement(2318..>3397)
                     /locus_tag="PKV59_05505"
     CDS             complement(2318..>3397)
                     /locus_tag="PKV59_05505"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46243.1"
                     /translation="LEVTTPSHRLDIGEGVIGKADVLEEIARLFGYEQIPTTRMADEL
                     PPAYVNRMVSFEEKLRDELASAGLQEIVNYRFTSPEREAKLLGKPAEKYVTLANPISP
                     DRSVMRTNLNVGMMEVVEKNIRLADTLEFFEIGPVFLPVEGEKRPHEEIHLSVAITGK
                     RIKDSWDTHYKDSLDFYDLKGITEVTAKGLHISGLRFEPAECAYMHPGKCAAVYAGEE
                     KLGILGELHPAIHKEYDLLSSPIILLDLNLEKMEPLSETIYKVHSISSFPAILEDIAV
                     VVDETITAGEVEAVIRQAGGKLLQSVELFDIFRNEQIGAGKKSLAYSLKYQSDEKTLT
                     DSDATAVRNKIIKRLDQVLGAKLRS"
BASE COUNT          927 a          822 c          644 g          904 t
ORIGIN      
        1 gcttaattcc acccgatcgt ggaatgtatt ttcagccgct tccaacctgt tcggatcttt
       61 atccaccaag gccgccaaat tcgttttttt ggaaagagag atattccgtg ccacattcga
      121 accccaataa ccgtaaccga tttgaacgat attaatcatt ttgatttgcc tgactcaacc
      181 cttcaaccga ctcgataatg aacgatggcc tgcctcgggc agcgtccaac gtccgccaaa
      241 gatattcggc gataacgccc aaggagatat tggtgatgcc gaaaccgatc atcaatatgc
      301 tgattaaaga aggccagccg agagtgactt cccgaatgtt gaaaagtttg atgataatta
      361 aataaagtgc gataatcgct ccgataatcg caaaaataaa ccccattgca cttaccatgc
      421 ggatgggaaa aaatgaaaaa gcgacaaacg aatcgatcat cagcttcatt tttttcccga
      481 aagaccattt cgatcgccca tgaacccgtt cccgatactc gacatccaaa gtcgtttgtt
      541 taaatcccag attcaaaacc tgcaatacca cagacgaatt ggattcgata ttccgattca
      601 attcccgttt gactttttca ttaaaaaaaa cggtattaac cccatccgga ggataacccg
      661 gaatcgcata cttacgaatc agccgggaat aagtattcga aaataatttc tccgatttgc
      721 cgactttgac attctttttc tgtacgcaga ccagatcgta cccttcccgg attttttgat
      781 atcccaatcg gataatttcc aacggttcct gtaaatcggc accgagccag acacagtttg
      841 gtgcggcagc attcaggaaa ccggctcgaa ccgccggatg cgaaccgaaa tttcgtgaga
      901 gtttgacaat ctttgcgctg aaattccgga agtgtacctt tcttaaaaga tccaaagagc
      961 cgtcggtcga gccatcatcg acgaaaacga cttccatcct aacgggcata gtcggagcaa
     1021 acgcttccaa ttcggcaatc aaatcgggta aattttcttc ttcgtttaaa aatgggataa
     1081 taacggaaat ttcaggttga tccatgcatt ttccttcggg ctcttgcagt tacgatatcg
     1141 tctcttattt tatcataggg agaatttgca tcaaaacttc gctttatttc gtctttattc
     1201 actcattttc tccgccttcc cgcccgtaaa aaacgaaatc ggttcgggaa caattacaga
     1261 taccgtgtac cgtgatcaat attcgattcc gtgttcttcg caaaaagttc ggatctgctc
     1321 ctccatagcc cggtaaaatg catccgaatc ggggaaatga gaaatcaacg acgaataatt
     1381 ccaacggccg aaatcgatcg aatcaacaaa actgatttct tccaaaattt cattnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntccaac ggccgaaatc gatcgaatca
     1561 acaaaactga tttcttccaa aatttcattc aaatcctgtg aaataacatt gggagtcggg
     1621 taaggttcca tgtgtaccca agtatgcatt cctttgttat ggagataacg taaagcatcg
     1681 attctttccc gatagggtgc cgcaccgggt tcacatcttt cgcgaaattc ttcatccaat
     1741 gaaaccagag aaattccgta agaatttcgt tgatccattt ccgcaagttc gggcggtaac
     1801 actccctttg tcaatatgcc gcacggaata ccgttttgat tgatcatgcg aattgacgct
     1861 aacgaaagct gccggacttc cggatatccg accatgaaag gatccgttgt caggcaaagt
     1921 tgaacatgat gaatcttcca atgccatttc cgaagctctt tttccaacag ttcaagggta
     1981 ttgccgacaa tagccggttg aatccattgg ctgtaactct tgacttttcc gaaacgcatc
     2041 gccatcagcc aggcataaca aggatatctg caaccgtggg cacatcccaa aaccggattc
     2101 atcgcataat ccagcgatga tataccggat ttatataaga ttgactttct gagaatactt
     2161 ttcatttttt aattgtaatt cgaaccgccg gcgatatttt caatacccgt gaaaacgtcg
     2221 ggggattcta atatagaaca gtacggataa atttcccgta ctgttctatg atccctcact
     2281 cgaaaggcac aaaccggtcg gtttgccgaa agagcattca gctccgcagc ttcgcgccca
     2341 aaacctgatc caatcgttta ataatcttgt tccgaacggc tgtggcatcg ctgtcggtta
     2401 atgtcttttc gtccgattga tatttcaaac tgtaagctaa gctctttttt ccggctccga
     2461 tttgctcatt gcggaaaata tcaaataatt caacgctttg cagcaacttt ccgccggctt
     2521 gcctgatcac agcttccact tctcccgcag tgattgtttc atccacgacg actgctatat
     2581 cttcgagaat cgccgggaac gacgaaatcg aatgtacttt atagattgtc tcggataacg
     2641 gctccatttt ttccaaattc agatccaaca gaatgatcgg agaagataaa agatcgtatt
     2701 ctttatggat agcgggatgt aattcaccaa ggatacccag tttttcctcc ccggcataaa
     2761 ccgctgcgca ttttcccggg tgcatataag cgcattcagc cggctcaaat cgcaaaccgg
     2821 aaatgtgcag accctttgcc gtcacttccg taatgccttt gagatcgtaa aagtccaagg
     2881 aatctttata gtgagtatcc caagaatctt ttatacgttt tccggtaatc gcgaccgata
     2941 aatgaatctc ttcatgaggt cttttctctc cttcgacagg caaaaaaacc ggaccgatct
     3001 cgaaaaactc caaggtgtcc gccagacgga tatttttctc aaccacttcc atcattccga
     3061 catttagatt cgtccgcatc acagaccgat cgggagaaat cggattcgcc agtgtcacat
     3121 atttttccgc aggctttcca agcaacttcg cttcccgttc gggagaggta aaacgataat
     3181 ttacaatttc ctgaagcccg gcggaggcta attcatcccg taatttttct tcaaacgaaa
     3241 ccatacgatt gacataggcg ggaggtaatt catccgccat tcgagtcgtc gggatttgct
     3301 catatccgaa aagacgggca atctcttcca aaacatccgc tttaccgatg acaccttctc
     3361 cgatatccaa ccggtgagag ggcgttgtca cctccag
//
LOCUS       DAOMIV010000197         3389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_29630_length_3389_cov_4.222855, whole genome shotgun sequence.
ACCESSION   DAOMIV010000197 DAOMIV010000000
VERSION     DAOMIV010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29630_length_3389_cov_4.222855"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKV59_05510"
     CDS             complement(<1..396)
                     /locus_tag="PKV59_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46244.1"
                     /translation="MRQLKYSFVTVFRTSLLFLIFLFLTGCNTPNSSSLQNSSSSQTP
                     QPDQTQIFANVLATANASIQQTILPENTEITMQVAELSETNIQDTELPAPTPDPNRTP
                     PALPATFTTDLLNKLDTPHTYIEDTCEFLK"
     gene            552..1034
                     /gene="smpB"
                     /locus_tag="PKV59_05515"
     CDS             552..1034
                     /gene="smpB"
                     /locus_tag="PKV59_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQP46245.1"
                     /translation="MIVKILYAKIKPMGEKIVATNRKAKFEYFILETFEAGIVLKGTE
                     IKSVRAGQISLADAYVQINDGKEAWLMNAHIAPYDPANRYNHDPLRPRKLLLNKREIR
                     NIWNSIRLKGMTIIPTKVYLSHGLAKVEIGIAKGKKLYDKREAIAKRDADRERARRDE
                     "
     gene            1027..2142
                     /locus_tag="PKV59_05520"
     CDS             1027..2142
                     /locus_tag="PKV59_05520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQP46246.1"
                     /translation="MSKVMRENKINRRDFLKIATISGGALLIPKNVHSYFDFAAQLDT
                     FPQSEFIGRNCLSGIMNFRVKPEADAEINKSVYEDFLFPIYREVVGEAPAGTYNATWF
                     ETPYGYVYSPGVQLVKNEPNVPETDLPDSSIGKGFWGVVTVPYVNLIFGKDPVSPWYI
                     SIKDHNPKLYYDQIFWIDEIKTASDGTILYRANELYGTYGDIVYADARAIRKITDEEV
                     APIHPEVENKRIYVNLTYQTLTCYENDVEVYFCRISSGAMYTNEGVLTDRYATPTGNH
                     HPWRKSISLHMSGSLTGSGWDTPGVPWNVIFATGGASIHGVFWHNAFGTPRSHGCVNA
                     KPDDAKWIWRWVKPEVGLDPGDITVSGDVGTLIEVKN"
     gene            2188..2823
                     /locus_tag="PKV59_05525"
     CDS             2188..2823
                     /locus_tag="PKV59_05525"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HQP46247.1"
                     /translation="MGTDKLSLLFKRFYILIFAFAFSLYTGLAFLAPVLMHYDLILPG
                     KLIYKFFGFFCHQLPYRSFFLFGEQAFYPLASANMDRGIITFEEATQNAKMDFFDLKS
                     FVGNPQMGYKVALCQRDIAIYLAIVLFCFIFLISNYHIPRLHWILWIIIGLVPIGLDG
                     FSQLFAAINPAVFPARESTPILRVITGAMFGFNTAWFLFPYLDKKLKEDEA"
     gene            2820..>3389
                     /locus_tag="PKV59_05530"
     CDS             2820..>3389
                     /locus_tag="PKV59_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HQP46248.1"
                     /translation="MTLVKESNGLRYYQFEEFAKLGVKHGFFTRLGGVSPVPFHSLNM
                     ATTVGDSNENVLENLNRMFQVFNLDLSTRYDGWQVHSSTTICVKSSRDVRTRTIRGDA
                     LLTDNPDVTLVMRFGDCVPVILMDPIFRVAGIYHAGWKGTVKKIGKNVVENMVKVYGC
                     NPQNIIAGIGPSIGPDHFVVQQDVSDQFIK"
BASE COUNT          968 a          625 c          784 g         1012 t
ORIGIN      
        1 ctttaaaaac tcacaggtgt cctctatata tgtatggggt gtgtccagtt tatttaataa
       61 atcggttgta aaggtcgccg gtaatgcggg aggggttcgg ttgggatccg gcgtcggagc
      121 cggcaattcg gtatcttgaa tattcgtttc ggacagctca gcaacttgca tcgttatctc
      181 cgtattctca ggcaagattg tttgttgaat agaagcattt gcggtcgcta agacatttgc
      241 aaaaatttgg gtctgatccg gttggggtgt ttgcgatgag gaactgttct gtaatgacga
      301 ggaattcgga gtgttgcaac cggtgaggaa caaaaaaatg agaaataata aagaagttcg
      361 aaaaacggta acaaaagagt attttaattg acgcacggaa cctatcctat ccttcattaa
      421 gaagtcaaat cattttgaaa tattataaat tattttcagg ttccaaaact tacttatcaa
      481 atgaaagaac tatccatttg agggaatgtt gatcttgtga ttgcgaaatt tacacatccg
      541 agggtatttc tatgattgtg aaaatccttt atgctaaaat aaaacccatg ggtgaaaaaa
      601 tcgttgcgac aaatcgaaaa gcaaaattcg aatattttat attggaaact ttcgaagccg
      661 gtattgtttt aaaaggcacc gaaataaaat cggttcgtgc aggtcaaatc agcttagccg
      721 atgcgtatgt tcaaatcaat gacgggaaag aagcttggtt aatgaatgct catatcgcac
      781 cttacgaccc cgctaatcgt tacaatcacg atccgctgag accgagaaaa ttgttactta
      841 ataaacgtga aatccgaaat atttggaaca gcatccggtt gaaagggatg acgattattc
      901 cgacaaaagt gtatctttcg cacgggttgg caaaagtgga aataggaatc gccaaaggga
      961 aaaaactata cgataaacga gaagcgattg caaaacgaga tgcagatcga gagcgagcga
     1021 ggcgtgatga gtaaagttat gcgagagaat aaaataaatc gtcgagattt tttaaagatt
     1081 gcgacaattt cgggcggagc tttattgatt ccgaaaaacg ttcactcgta ttttgatttt
     1141 gcagctcaat tggatacttt tcctcagtcc gaatttatcg gacgaaattg tttgagcggg
     1201 attatgaatt ttcgagtcaa gccggaagcg gatgccgaaa tcaataaaag cgtatatgaa
     1261 gatttccttt tcccgatata ccgggaagtt gtgggagaag caccggcagg aacatataat
     1321 gccacttggt ttgaaactcc ctatgggtat gtttattctc cgggtgtgca attggtaaaa
     1381 aatgagccga atgttccgga aaccgattta cccgattcaa gtatcgggaa aggattttgg
     1441 ggagtggtta cagtccctta tgtcaatttg atattcggaa aagatccggt ttccccatgg
     1501 tatatttcta taaaagatca caatcccaag ctttattacg atcaaatatt ttggattgac
     1561 gaaatcaaaa ctgcttcgga tgggactatt ctttatcggg caaatgagct ttacgggact
     1621 tatggcgaca ttgtctatgc ggatgctcgg gcaattcgaa aaatcacgga tgaagaagtg
     1681 gcaccgattc atcccgaagt agaaaataaa cgaatttatg tgaacctgac ttatcagaca
     1741 ctaacgtgct atgagaacga tgtggaagtt tatttctgcc gtatttcctc aggagctatg
     1801 tatacaaatg aaggggtgct tacggataga tatgccacac ctaccgggaa tcatcatccg
     1861 tggagaaaat cgatttcgtt gcatatgtcc ggcagtttga ccggttccgg ttgggatact
     1921 cccggagtac cttggaatgt catatttgct accggcggcg cttcaatcca cggtgttttt
     1981 tggcataacg cgtttggaac tccgcgatcg cacgggtgtg tcaatgcaaa accggacgat
     2041 gcgaaatgga tctggcgttg ggtaaaaccg gaagtcggtt tggatcccgg cgatattacc
     2101 gtatccggag acgtggggac tttaattgag gtcaaaaact gatggatata aacacccgaa
     2161 cttttttatt gatttttcga aaaaacaatg ggaaccgata agttatcctt gctttttaaa
     2221 cgcttctata tcttgatctt tgctttcgct ttttctcttt ataccggttt ggcgtttctg
     2281 gcaccggtat taatgcatta tgatttgatc cttccgggaa agttgatata taagtttttc
     2341 ggtttttttt gtcatcaact tccttaccga tctttttttc tgttcgggga gcaggcattt
     2401 tatccgcttg catcggcgaa tatggatcgc ggcatcatca ctttcgaaga agcaacccaa
     2461 aatgcgaaaa tggatttttt tgatttgaaa tcttttgtcg ggaatccgca gatgggctat
     2521 aaggttgctc tatgccaacg ggatattgcc atttatcttg ccattgttct tttctgcttt
     2581 atttttttga tttcaaacta tcatattccc agattgcatt ggatcctgtg gattattatc
     2641 ggattggttc cgatcggatt agacggcttt tcacaattgt ttgctgcgat caaccccgct
     2701 gttttccccg ccagagaaag cacgcctatt ttgcgtgtaa tcaccggtgc gatgtttggg
     2761 ttcaatacgg cgtggttttt atttccttat ttggataaaa aattaaaaga ggatgaagca
     2821 tgaccttggt taaggaatca aacggattac gatactatca gtttgaagaa ttcgcaaaat
     2881 tgggagttaa gcacggtttc tttacccgat tgggaggagt cagtccggtt ccttttcatt
     2941 ctctcaatat ggctacaacg gtcggcgaca gcaatgaaaa tgtccttgaa aatttgaacc
     3001 gaatgtttca agtttttaat cttgatctat ctactcgtta tgacggatgg caggttcaca
     3061 gttccacaac gatctgcgtg aagtcatcaa gagatgttcg aacccgaact attcgcggag
     3121 atgcactatt gaccgataac ccggatgtca cactggttat gcgctttggc gattgtgtcc
     3181 ccgttatttt aatggacccg atatttcgtg tagccggaat ctatcatgcg ggctggaaag
     3241 gaaccgttaa gaaaatcgga aaaaatgtgg tagaaaatat ggtcaaagtc tacggatgca
     3301 atccgcaaaa tatcatagca ggaataggtc cgtcaatcgg accggatcat tttgtcgttc
     3361 agcaagatgt ttccgaccaa tttattaaa
//
LOCUS       DAOMIV010000198         3382 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_29707_length_3382_cov_4.177337, whole genome shotgun sequence.
ACCESSION   DAOMIV010000198 DAOMIV010000000
VERSION     DAOMIV010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29707_length_3382_cov_4.177337"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="PKV59_05535"
     CDS             <1..960
                     /locus_tag="PKV59_05535"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQP46249.1"
                     /translation="VIGKSGCSIGGSTVDRWIFEEALMQNRRDFSDPVIHRDGRIILA
                     ACEQLKERLSFFEEADLSLILTDHRKFTLRQSREDLETLLRKRGLFQSVDAVVRDALL
                     QAADHGIDTSAITALIPVGGGSMIPAIRSELENVLRKALVLNGDPMTAVAKGAINLID
                     GFGVLDFVRHQYAVRIRDGKTGEFRFHTIIQPGTPYPSDEPVTTLKIKASRQNQSLFG
                     LAIYEISQPAFRPEAQTEIFFDEEGAIRLFSRADSEKEGEDTFWLNEHAPLFLHADPP
                     AEKGIARFKAEFKVDRNKMLLITVYDLSSGLCLFEDYPVIRLN"
     gene            969..1190
                     /locus_tag="PKV59_05540"
     CDS             969..1190
                     /locus_tag="PKV59_05540"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HQP46250.1"
                     /translation="MAQLEEIEILIDEKGKVTFQVRGIPGKACLDTTKQLEEALGNEV
                     IKREYTDEYYSDKPVENRSRLRLKTKREL"
     gene            1190..1759
                     /locus_tag="PKV59_05545"
     CDS             1190..1759
                     /locus_tag="PKV59_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HQP46251.1"
                     /translation="MNIRWRYFIPESSANGPGKRCVIWFQGCSIDCEGCCNPDLQDPK
                     GGRSDPVETVFEKIRLVAKNVHGITISGGEPFQQPEALCELVRMIKAETTLSVFVFSG
                     YQKEKILSDPKRNRCLPWIDAILYGPYIHSNPPALERFCASGNQGLWLLSDRFQAEDF
                     SDLPVLEFLIGEKGELIQSGLYGINFIEK"
     gene            1924..3276
                     /locus_tag="PKV59_05550"
     CDS             1924..3276
                     /locus_tag="PKV59_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46252.1"
                     /translation="MKKKLLSIILSLFIFLAIPNFSGLAQSSVTQYIVNLYFFRGEGC
                     PHCAQEEVFLQQMTAKYGDQLVIHEYEVWYHPENVTLLEKFAEKYGFEPSGVPVTFIG
                     NQYWVGFNDIKQQEIETAISSGIQNGVVDTQDIVDGKTELIPPEKALSAKITIPLIGQ
                     IDLAQETLFVSTVIIGLVDGVNPCSLWVLTMLLAMLVHTNSRKKMTIIGLVFIIVAAL
                     IYALFITGVFTLLTYASYLKWIQIVVAAITLVLGLINLKDYFFFKEGVSLTIADEKKP
                     GIYQKMRNVVNNSENLWAMIGATIVLALGVSLVEFSCTAAFPVVWSNLLVANHASKAT
                     FIALLILYLFLYQLDELVIFTITVISMKSSRMQETHGQILKLFSGCLMVSLSLVMLID
                     PTLMNNLSSSLLVFGVAILITAIIYLVTDKILPRYGIRIGHTRKSDPVKGKPVRKKSK
                     "
BASE COUNT          967 a          653 c          791 g          971 t
ORIGIN      
        1 gtgatcggga aatccggttg ttccattggg ggatcaacgg tagaccgttg gatatttgaa
       61 gaagcgctga tgcagaatcg aagagatttt tccgatccgg tgattcatcg ggacggacga
      121 attattttgg cggcatgcga gcaattgaaa gagcgactgt cctttttcga agaagcggat
      181 ctgtcattaa tcctaacgga tcatcgaaaa tttactttgc ggcagtctcg ggaagatctt
      241 gaaaccttat taaggaaaag aggattgttt caatcagttg acgccgtagt tcgggatgcg
      301 ttgttacagg cagccgatca cggtatcgat acctctgcga ttaccgcgtt aattccggtc
      361 ggcggaggca gtatgattcc tgcgatccgt tccgaattgg aaaatgttct ccgtaaagcc
      421 ttagtcttaa acggtgaccc gatgactgcc gtcgccaaag gtgcgataaa tttgatcgat
      481 ggtttcggag ttttggattt tgtgcggcat caatatgcgg ttcggattcg ggacggcaaa
      541 accggtgagt tccgttttca tacgattatt caacccggta ccccttatcc ttccgatgaa
      601 ccggttacca ctctgaaaat taaggcgtcc cgacaaaatc aatccctttt tggtttggca
      661 atctatgaaa tcagccaacc tgcttttcga ccggaagcgc aaacggaaat atttttcgac
      721 gaagagggcg caatcagact tttttcccga gcagattccg agaaagaggg agaagatact
      781 ttttggctca atgaacatgc cccgctattc ctgcatgcgg atcctcccgc ggaaaaaggg
      841 attgccagat tcaaagcgga atttaaagtc gaccgcaata aaatgttatt gataaccgtt
      901 tatgatttat catccggctt atgtcttttc gaagattatc cggtcattcg tttaaattag
      961 gagcataaat ggcgcagctt gaagaaattg agatattgat tgatgagaag gggaaagtaa
     1021 cctttcaggt tcgtggaatt ccggggaagg cttgtttgga tacgacgaaa cagcttgagg
     1081 aagccttggg gaatgaggtc atcaagcgag aatataccga tgaatattat tcggataaac
     1141 cggttgaaaa tagatcccga ttacggttaa aaacgaaaag agaactttga tgaatatccg
     1201 gtggcggtat tttattcccg aaagttcagc gaacggaccc ggcaagcgct gcgtgatttg
     1261 gtttcaggga tgttcgatag attgcgaagg ttgctgtaac cccgatttgc aagatccgaa
     1321 aggcggcaga tcggatccgg tagaaaccgt tttcgaaaaa atccgtttgg tcgcgaaaaa
     1381 tgtgcacggt atcacaatca gcggcggtga accatttcaa caacctgagg ctttatgtga
     1441 actggtaaga atgataaaag cggaaacgac tctttccgtt tttgttttca gcggatatca
     1501 aaaagagaaa atactgtctg atcccaagcg aaatcgttgc ctgccatgga tcgatgcgat
     1561 tttatacgga ccgtatattc attccaatcc gcctgctttg gagcgattct gcgcatcggg
     1621 aaatcaagga ttatggttgc tcagcgaccg atttcaagcg gaagatttca gcgatttacc
     1681 ggttttggaa tttttaatcg gggagaaagg tgaactcatt caaagcggac tttatggcat
     1741 caattttatt gagaagtgaa aaaagaattt cgcgaaactc atcaaaatcg ttctccggaa
     1801 ttcaaatggt ttgaaaagtt tttgaaaagt aagaatcaga tcattgatag aaaaagaccc
     1861 taataaccct tttacggtaa aattgtagcg gtggcaaatt tgaaggtatg aagccggact
     1921 tctatgaaga aaaaattact ctcaattatt ctcagccttt ttatatttct tgcgataccg
     1981 aatttctccg gactggcaca gagttccgta acccaatata tcgttaattt atattttttt
     2041 cggggagaag gctgccctca ttgtgcacag gaagaggttt ttttgcaaca gatgaccgca
     2101 aaatatggcg atcaactggt gatacacgaa tacgaagtct ggtatcatcc ggaaaacgtg
     2161 acacttctgg aaaaatttgc cgaaaaatac gggtttgaac cttccggagt tccggttact
     2221 tttatcggaa atcaatattg ggtcggtttc aatgatatca aacagcagga aatcgagacg
     2281 gctatttcga gcggaattca gaacggggtg gttgataccc aagacattgt tgacggaaaa
     2341 acggaattaa ttcctcccga aaaggcttta tccgccaaaa ttaccattcc tttgatcgga
     2401 cagattgatt tggcacagga gactttattc gtcagcacag tcattatcgg attggtggat
     2461 ggcgtaaatc cctgttcctt atgggtatta acgatgcttt tagccatgct ggtccatacc
     2521 aattctcgga aaaagatgac gatcatcggc ttggttttta ttatcgtggc cgcattgatt
     2581 tacgctttat tcataaccgg tgtcttcaca ctgttgactt atgcgagtta cctgaaatgg
     2641 atccagattg ttgttgccgc aattactttg gtattgggtt taattaatct gaaagattat
     2701 ttctttttca aagaaggtgt ttcactgacg attgccgacg aaaaaaagcc gggtatttat
     2761 caaaaaatgc gcaatgttgt taataacagt gaaaatcttt gggcaatgat cggagcgacc
     2821 attgttctgg cgttaggagt ttcgttggtc gaattctcct gtaccgccgc atttccggtg
     2881 gtttggagta acttgctggt cgcaaatcac gcatcaaaag caacttttat cgcattattg
     2941 attctttatc tgtttctcta tcaactggat gaattggtaa ttttcaccat tacggtaatc
     3001 tcgatgaaat cgtcccgaat gcaggaaaca cacgggcaaa ttctaaagct tttcagcgga
     3061 tgccttatgg tttctctttc gttggtgatg ttaattgatc cgactttgat gaacaatctt
     3121 tcgagttcat tgcttgtttt tggggttgca atattgatca cggcaatcat ttacttggtc
     3181 acggataaaa tcttacccag atatggaatt cggatcggtc atacccgaaa atcggacccg
     3241 gtaaaaggaa agccggttcg caaaaaatcc aaataaactc aaaccgctct ttataactcc
     3301 tgcgaggaac ttcaaaaaaa cgggaaaaag aacatcgaat aaaaaatcct acgattaaga
     3361 ggatttaagt gctgtaatat tt
//
LOCUS       DAOMIV010000199         3365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_29953_length_3365_cov_3.074924, whole genome shotgun sequence.
ACCESSION   DAOMIV010000199 DAOMIV010000000
VERSION     DAOMIV010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29953_length_3365_cov_3.074924"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..846
                     /locus_tag="PKV59_05555"
     CDS             163..846
                     /locus_tag="PKV59_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQP46253.1"
                     /translation="MMKIRYLGHSCVEIIGSHHILIDPDFTFPPQPGVEYICLTHAHK
                     DHIGKVAEVQSGTILASADICEIVERRGIPSSRIRPVSPGQEIDSITILPGYSLTNQP
                     AYVFMNLLTRRRLPEPGGTPLSFLVRDEISVLHVGDANEAPLNVHPDIFCLPWRTSPV
                     GDKKYKESLIKLANSFQPNYLLPIHYDLPGSEADPAEICKYTTSMKVLLGKFWFLFRN
                     REEYVTEDF"
     gene            867..1715
                     /locus_tag="PKV59_05560"
     CDS             867..1715
                     /locus_tag="PKV59_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQP46254.1"
                     /translation="MKQKVAIVTDGACSLTLEQANEYGIHMAPIYVNIDDKTYIAGVN
                     LGGPEFYQLQRASKKLPTTSQTTAEDFLKIFTKLADEAEEIVTITISSKMSATIQSAE
                     MAKKQFTNVPVHIIDSESVSLGQGMMAIAAAQAAKEGQDAESVIHLVEEIKKRMNVIF
                     TVRTLEYLYKGGRIGGATAFLGSALAIKPILYIKDGVIEPLEKQYTRKKSIMRLVEIM
                     KERIGDKPVHVAILHGDAEEEAYVLENQIREKFNYVQLIKAEIGPVIGVHAGPGTLGL
                     VFYSME"
     gene            1756..2523
                     /locus_tag="PKV59_05565"
     CDS             1756..2523
                     /locus_tag="PKV59_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQP46255.1"
                     /translation="MQIVYLIMIGLITGCVVGLTGASGVVVVVPLLTVLMGFPVHMAI
                     GTSLFIDTITPLFVAWSYYKSGNINLKAGLWLALGSVTGAQIGSSFANEAISGQGMSI
                     GFIIIIFLMAIGMWVKSTRPERPTVEKSQIKPFTVKNAAISFGIGLALGIFSGMFGAG
                     GGIAFLIVMITVLKYSTHVAIGTSSLIMSLTAASGTIGYAIHGNVNFGSGLIIAVGSV
                     VGGLLTARLANKVNEQTLEKLIGCVFAGLGVIMLTIQ"
     gene            complement(2570..3211)
                     /locus_tag="PKV59_05570"
     CDS             complement(2570..3211)
                     /locus_tag="PKV59_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HQP46256.1"
                     /translation="MEEKLFSVTISHQIGSGGAYIGEKLSERLGIPFLDRAILKEVSN
                     QLNLAESELEHREERLSSFWENFNRLVAYTNPTTSLTSQTYFPTDKDLFQVECDTIKR
                     IAEKSSAIFLGRCGYYVLRDHPRKVSIMLIADQSSRVKRLQELYKISEKEALEMIRTN
                     DRERSEYIRTFTKQDWLDVRLYDICINTTSVGLDCAADLAENCIRAKLRLTEK"
BASE COUNT          976 a          625 c          748 g         1016 t
ORIGIN      
        1 gcggatcgta aagaaggaac catctcattt ggagaagaat atataagtat ttttgagatc
       61 atctcagtgt attttcctca aagggaataa agaataaatt tcgagggtat taccgaaaaa
      121 gggaatgaaa atgacaaatc ggcaatgaat caggaatagg caatgatgaa aatacgttat
      181 ttaggtcatt cttgtgtcga aatcatcggg agtcatcata ttttgatcga tcccgatttt
      241 acttttcctc cccaaccggg tgttgagtat atttgtttaa ctcatgcgca taaagatcat
      301 atcggaaagg ttgcggaagt tcaaagtgga acaattttgg cgtcggcgga catttgtgaa
      361 atcgtagagc ggagaggaat tccttcttca cgaatccggc cggtttcacc ggggcaagaa
      421 atcgattcga tcacaatcct tcccggttac agtctgacca atcaacccgc ttatgttttt
      481 atgaatttac tgactcgcag aagacttcct gaaccgggag gaactccttt atctttttta
      541 gttcgggatg aaatcagtgt tttgcacgtg ggtgatgcaa atgaagcacc tttaaacgtt
      601 catccagata ttttctgctt gccttggcgc acctctcctg taggagacaa aaaatataaa
      661 gaaagcttga taaaattggc aaacagtttt caaccgaatt accttttacc cattcattat
      721 gatttaccgg gttccgaagc ggatcccgct gaaatttgta aatataccac ctcaatgaaa
      781 gtccttctcg gaaaattttg gtttctattt cgcaatcgag aggaatacgt aacagaagat
      841 ttctgatctt cggaaaggaa aagattatga agcaaaaagt tgctattgta acagacggtg
      901 cgtgttcttt aacgttggaa caagcgaacg aatacggaat tcatatggca cctatctatg
      961 ttaatattga cgataaaact tacattgccg gagtcaacct tgggggcccg gagttttatc
     1021 aacttcaacg tgccagtaaa aaattaccga ctacctcaca aactaccgct gaagattttc
     1081 ttaaaatctt tacaaagctt gcggatgaag cggaggaaat tgtgacgatc acaatttcga
     1141 gcaaaatgag tgcaacaatt caatccgcag aaatggctaa aaaacagttt acgaacgtgc
     1201 cggtacacat tattgattcg gaatcggttt ctttggggca ggggatgatg gcaattgccg
     1261 ccgctcaagc tgccaaagaa gggcaagatg cggaatctgt tattcattta gtcgaagaaa
     1321 tcaagaagag aatgaatgtt atttttacgg tccgaacttt ggaatatctt tataaagggg
     1381 gacggatcgg gggagctacg gcatttctcg gttctgcttt ggcgatcaaa ccgattcttt
     1441 atattaaaga tggtgtaatc gaaccgttgg aaaaacaata tactcgtaaa aaatcaatta
     1501 tgcgcttggt tgaaatcatg aaggaaagaa tcggagataa gccggttcat gtagccattc
     1561 ttcatggaga tgccgaggag gaagcttatg ttttggaaaa tcaaattcgc gagaaattta
     1621 attacgttca attgattaaa gcggagatcg ggccggttat cggagttcat gcgggtcccg
     1681 gtactttagg gttggttttt tatagtatgg aatagagcat atttttttaa aaatcatatc
     1741 agaggagaaa tattgatgca aattgtttat ttaattatga tcggattaat aaccggatgc
     1801 gtggtgggat taacaggagc cagcggtgtt gtagtcgttg tgccccttct gacggttttg
     1861 atgggttttc ctgtacatat ggcaatcgga accagtttgt ttattgatac aattacacct
     1921 ttattcgtgg catggtccta ttataaaagt ggcaatatta atcttaaggc gggtttatgg
     1981 ttagctttgg gttccgtaac cggcgctcag atcggctctt cctttgcaaa tgaagcgatt
     2041 tcaggacaag gtatgagcat cggatttatt attatcattt ttttgatggc aatcggaatg
     2101 tgggtaaaat caactcgtcc ggaaaggccg accgttgaaa aaagccaaat taaaccgttt
     2161 acggtaaaaa atgcggctat ctcattcgga atcggattag cgttgggtat attcagcgga
     2221 atgtttggcg cgggcggcgg gatcgcattt ttaatcgtta tgataaccgt tctaaaatat
     2281 tccactcatg tggcaatcgg cacttcttct ttgatcatgt cgctgaccgc agcatcgggg
     2341 acaatcggat atgctatcca tggaaatgta aatttcggtt cggggcttat cattgcagtc
     2401 ggatcggttg tcggggggtt attaaccgct cgcttggcaa ataaggtcaa tgaacaaaca
     2461 ctcgaaaagc tgatcggatg tgtttttgcc ggcttaggtg ttattatgct gactatacaa
     2521 taattgtgtt tcatttaaat ctaaccggca agaaaattgc cggttagatt tatttttccg
     2581 ttaatcgaag tttcgctctg atacaatttt cggccagatc cgctgcacaa tccaatccta
     2641 cggatgtggt attgatacag atatcataaa gacgaacatc caaccaatcc tgtttcgtaa
     2701 aagtgcgtat atattccgat cgctcccgat cattggttcg gatcatttct aaagcttctt
     2761 tttcagatat tttatacaat tcttgtaaac gttttaccct tgacgattgg tcagcgatca
     2821 acatgatgct gacttttctg ggatgatccc gaagaacgta atagccgcaa cgtcctaaaa
     2881 agatagctga ggatttttcg gcaatgcgct tgatcgtatc acattccact tggaataagt
     2941 ctttgtcggt agggaaataa gtttgcgagg ttaacgaggt tgttggattt gtgtatgcga
     3001 ccaagcgatt gaagttttcc caaaaagaac ttaaccgttc ttcacgatgt tccaattcag
     3061 attcagccag attcagttgg tttgatactt ccttcaaaat tgcacgatcc agaaagggaa
     3121 tacctagccg ttcgcttagt ttctctccga tgtaagcgcc tccgctgcct atctgatgac
     3181 ttatagtgac agaaaaaagc ttttcttcca tggttttcct ccctttttat aaaagaagaa
     3241 cataggcata tttatattat agcaatatgc ctatattcta tttttcagaa cgtatcaaat
     3301 tgtctgaccc atttgaaact ggctgttata aagctctgca taaaagcctc cgcgagccaa
     3361 aagtt
//
LOCUS       DAOMIV010000200         3361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_30010_length_3361_cov_4.451301, whole genome shotgun sequence.
ACCESSION   DAOMIV010000200 DAOMIV010000000
VERSION     DAOMIV010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30010_length_3361_cov_4.451301"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..828)
                     /locus_tag="PKV59_05575"
     CDS             complement(184..828)
                     /locus_tag="PKV59_05575"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HQP46257.1"
                     /translation="MVDAEKYEITIGNYDAKILVPEIKGLADTKQQDQLNGSFSDHAG
                     ELVQNFISEAKSIRQQFPEDGPHFELQYQYEVLYDNEDNMVFRVFEFTVAGSSNYMAQ
                     YFTVDKRAHKLLSLSDITGGKADAMEKIRAYIEESMREYNKNNDMKYWVDDGPEGGDY
                     KESLDKVFASIEEKRQYYLNEEGQLVITFNKYDVAPGAMGSPEFIIPNEILGNN"
     gene            complement(1211..>3361)
                     /gene="nifJ"
                     /locus_tag="PKV59_05580"
     CDS             complement(1211..>3361)
                     /gene="nifJ"
                     /locus_tag="PKV59_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQP46258.1"
                     /translation="PIHAPYLIGENDANFLALHMFTFLEKLDITRFAAKGGTFLLNSF
                     YGPDEVWDHLPAEVQKDIIDKELKFYVIDAFSVANKTGMGGRINTIMQTCFFAISGVL
                     PRDEAIEEIKKSITKTYGKKGDKVVAQNFEAVDSTLANLFEVKYPKQVTSKIHRLPAV
                     PPEAPEFVQKTTAPMIAAQGDKLPVSALPEDGTYPSGTSQWEKRNIALVSPVWDPATC
                     IQCGKCSMVCPHAVIRQKVYDASYLKNAPETFKSVDAKGFKEFPGAKFTLQVSVEDCT
                     GCGLCVYTCPAKNKENPQLKAINMAEQLPQRATESKNWAFFLTIPDPDRTKIVPNSVK
                     LAQQLRPLFEFSGACAGCGETPYIKLVSQLFGDRAIIANATGCSSIYGGNLPTTPYTF
                     NNEGHGPAWSNSLFEDNAEFGLGMRLTVDKQNQFAKELIADLRGEIGDQLADGLLNAD
                     QSTEQGINEQRKRVKDLKDKLSKSKDPKAANLISIADALIKKSVWIIGGDGWAYDIGY
                     GGLDHVLASGKNVNILVLDTEVYSNTGGQASKSTPMAAVAKFASKGKDLPKKDLAYIA
                     MTYGYIYVAKVALGANDNQVLKAMLEAEAYDGPSLIIAYSHCINHGINMSLGLEQQKK
                     AVESGHWPLFRYNPTKIEQGENPLTIDSKAPSIPLSDYAYSENRYKMLLKSDEARAEM
                     LMKNAEKESGRRWELYKQLAELNYKTGVAGPKNE"
BASE COUNT          927 a          768 c          642 g         1024 t
ORIGIN      
        1 aaagactcgt tggcaataca tcggtgatta tctgaccctc gccaccttga tttttataat
       61 tgttgcatta cttcatcaac gacgaaaaaa taaaaaaatg aaagcagaaa attaagtctc
      121 tttcgtttta aatagaaaag gtgcctctat tccccctgag gcaccttttt tgttaattaa
      181 gatttagttg ttccccaata tctcattcgg aataataaat tcgggactcc ccattgcacc
      241 cggtgccacg tcatatttat tgaaagtgat taccagttgc ccttcttcat tgaggtaata
      301 ttgcctcttc tcttcaattg aggcaaaaac tttatccagc gattctttat aatctccgcc
      361 ctcaggaccg tcatcaaccc agtatttcat gtcgttgttt ttgttatatt cacgcataga
      421 ttcttctata taagctcgaa tcttttccat tgcatccgct tttcctccgg ttatatctga
      481 taacgataag agtttgtgcg ctcgtttatc aacggtgaag tattgtgcca tataatttga
      541 agaacccgca accgtaaact caaaaactcg aaaaaccatg ttatcttcat tatcatagag
      601 tacttcgtat tgatactgaa gttcaaaatg aggaccgtct tccgggaatt gttgtcgaat
      661 ggattttgct tcactaataa aattttgtac taattcgccc gcatgatcac taaaagagcc
      721 atttaattga tcttgctgtt tcgtatcagc gagtcccttt atttcgggaa cgaggatttt
      781 tgcatcgtag ttgccaattg taatttcata tttttcagca tcaaccactt tgagactccc
      841 gggttccgcc gaaacgaata caaaaacagc taaaaaacag acaagaacaa taaaaaaaga
      901 aagactgaca ttttttttca tagaatttcc tttcatacat tttttcatta tcaatacgcc
      961 cactccgtta ttttagttcc ttatcacctg atgaacgaga caaatggtct taaacaaatt
     1021 ggtgagagag cagagttatt tttacatttt tcccgatagt atcttgaaga atttcaagcg
     1081 attaatcttt atattatttt tcttcttcga ttgatatcat ccgaaaatca atttagaaat
     1141 tttaaccaaa agaaggctga aaaacagcct ccttttgatg tattatttat tcaaaaaata
     1201 agcaacttcc ttattcgttc ttgggtcccg caacaccggt tttgtaattc aattcggcta
     1261 attgcttata caattcccag cgtctgccgg attctttttc ggcgttcttc atcaacattt
     1321 ccgcccgagc ctcatcactc ttcagcaaca tcttataacg attttcgcta taagcataat
     1381 ctgatagcgg aatggaaggc gcttttgaat caatcgtcag aggattctca ccttgctcta
     1441 ttttggttgg gttatagcgg aacaaaggcc aatggccgga ttcgacagct ttcttttgtt
     1501 gttccaatcc cagactcata ttaattccgt gattaataca gtgtgaataa gcgatgatca
     1561 gagacggtcc gtcgtaggct tccgcttcaa gcatggcttt caaaacctga ttgtcattcg
     1621 cacccaacgc cactttcgct acatagatat aaccataagt catggcaata taagccagat
     1681 ccttcttggg cagatctttt cctttggacg caaatttagc aactgccgcc atcggggtag
     1741 acttggaagc ctgtccgccg gtatttgaat acacttcggt gtcgagtaca aggatgttaa
     1801 catttttccc cgatgccaaa acatgatcca gaccgccata accgatatca tatgcccagc
     1861 catcgccacc gatgatccag acactctttt tgattaaagc atcggcgatt gaaatcaaat
     1921 ttgccgcttt aggatctttc gatttcgaaa gcttatcctt caaatctttg acacgctttc
     1981 gctgttcatt aattccttgt tcggtggatt gatcggcatt gagtaaaccg tcagccagct
     2041 gatcgcctat ttctccacgt aaatcagcaa tcagttcttt tgcaaactga ttctgtttgt
     2101 caacggtcaa tcgcatgccc agtccgaact cagcgttatc ttcaaatagg ctgttcgacc
     2161 atgccggccc gtgtccttca ttgttgaagg tataaggagt tgtcggcaag ttaccgccat
     2221 aaatggagga acaaccggtc gcatttgcga ttatggcacg atcgccaaat aattgcgaga
     2281 ccagtttaat gtatggggtc tcgccgcatc cggcgcaggc gccggaaaat tcaaataagg
     2341 ggcgcaattg ctgagccaat ttaaccgaat tcggaacaat ttttgtccga tccggatcgg
     2401 gaatggtcag gaaaaatgcc caattcttcg attcggtggc acgttgtggt aactgttcag
     2461 ccatatttat ggctttcaac tgcggatttt ccttattttt tgccgggcaa gtatagacac
     2521 acaaaccgca gccggtacaa tcttcgaccg agacctgaag ggtgaatttc gctcccggaa
     2581 attctttaaa acccttcgca tcgactgatt tgaaagtttc cggagcattc ttcagataag
     2641 aagcatcata aactttttgt cgaataaccg catgcggaca gaccatggaa cattttccac
     2701 attggataca ggtcgccgga tcccataccg gagaaaccaa cgcaatattc cgtttctccc
     2761 attggcttgt ccccgacgga taagttccgt cttcagggag ggcggaaacc ggcaatttgt
     2821 cgccttgggc ggcaatcatc ggtgctgtgg tcttctgaac aaattcaggt gcttccggcg
     2881 gaacagccgg cagtcgatga attttactgg tgacctgctt gggatatttc acctcaaata
     2941 aattagcaag ggtagaatca accgcttcaa agttctgagc gacgacttta tcacctttct
     3001 tcccatatgt ttttgtaatg gactttttaa tttcctcgat tgcttcatcg cgcggcaaga
     3061 caccggaaat tgcgaaaaag caagtctgca taatggtatt aatccttcca cccatgcctg
     3121 tcttattcgc aaccgagaaa gcgtcaatga catagaactt taattctttg tcaataatat
     3181 ctttttgtac ttcggcggga agatgatccc aaacttcgtc aggaccgtaa aacgagttca
     3241 ataggaatgt tccacccttc gcagcgaaac gggttatgtc caacttttca agaaaagtga
     3301 acatatgcaa tgccaagaaa tttgcatcat tctcaccgat caaatacgga gcatgaatcg
     3361 g
//
LOCUS       DAOMIV010000201         3345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_30203_length_3345_cov_4.605167, whole genome shotgun sequence.
ACCESSION   DAOMIV010000201 DAOMIV010000000
VERSION     DAOMIV010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30203_length_3345_cov_4.605167"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /gene="fmt"
                     /locus_tag="PKV59_05585"
     CDS             complement(<1..706)
                     /gene="fmt"
                     /locus_tag="PKV59_05585"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQP46259.1"
                     /translation="MSERAPRIVFMGSPEIAVPTLRGLAQFYPVVGVVTQPDRPAGRG
                     RLPKPPAVKLAAAAEGIPVIQPEKMKDPGVFETLQEWNPDLIVVMAFGKILRKKVLDL
                     PRYGCINIHASVLPRWRGASPIQAAILAGDSETGISIMQMDSGMDTGAVYAIERCPID
                     PQDTAATLSEKLANLGADLFLRSLPLILDGILLAKPQSDEGVTYAPLISKEDGKLDFR
                     LPAFQSERKIRAYNPWP"
     gene            complement(897..3029)
                     /locus_tag="PKV59_05590"
     CDS             complement(897..3029)
                     /locus_tag="PKV59_05590"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQP46260.1"
                     /translation="MEDKTAEKKIYSSGYAGTIPGVHKEINEDFVIFFEKEPEGTVGA
                     SNLYVLTDGVTGTFHPEITARFAGKKILYEYFKSADFIDANKLALAMRNANNEIYAYA
                     ELKQEKMATVALATAITDGNAVIGTIGDSRVYVVRNDKVYQITEDQNILEEKVRKGEL
                     TKEEEFETDAQKPIIRALGSEKDIVVDIYDGIEVKPGDVLLMCSDGLTAYIGKDEILE
                     AVQADTPKEIVRNLLAYAANQNSPADASAIAIKIYDENTIQTVVRQPGTLPVDTDLNK
                     ETKELDLMMKSKPRHQPEKTEKKGTKKTSAYIISALLICLVIGGIFWFFSDSEGRPSL
                     FGPRMTATATVDPVQATLAIIHQTQQAEAIARLSATPTATLTPMPTMTSTVTVVPTLF
                     DLNVPVAGAVTEAADFGGTQTVLTPGTAEVQVTITPTPVDKGPIVSEKDQAEMVYVKA
                     GEFLLGSDPLKDGYANTIEETPQITVYLDGFWIDKYEVTNARYLKCVQAGGCTQSSYM
                     LLNDPTYADMPVTYVTVEEAEQFCEWAGKRLPTEIEWEKAARGTDGRIYPWGDEEPVE
                     GTRYANYPGYTDPDAQQVSGLFKVGSFPEGASPYGAMDMAGNVWEWTSSKYQADYYQT
                     LVDEAETGTEVIRNPKGSQSGIANVIRGGSAAETEINNYLAYLRTANRSYVNMNSSYY
                     IGFRCVLPDSESTGETGAVVQESVKSEE"
     gene            complement(3122..>3345)
                     /locus_tag="PKV59_05595"
     CDS             complement(3122..>3345)
                     /locus_tag="PKV59_05595"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HQP46261.1"
                     /translation="MFASTVRIRNGVHPVLPVTVTQPFPKDRLKDLSAELRKTEVTAP
                     VRMGDVIVKNVLGTGIDVIASRDMESVRS"
BASE COUNT          812 a          870 c          699 g          964 t
ORIGIN      
        1 tcggccaagg attataggca cgaatttttc tttccgattg aaaagcaggc aaacggaaat
       61 caagttttcc gtcctctttg gatatcaacg gtgcataagt gacgccttca tccgattgtg
      121 gttttgccaa taatattcca tccaaaatca atggcaaaga tcgaaggaat aagtcggctc
      181 ccaaatttgc caatttttcc gacagggtag cggcggtatc ttgcggatcg ataggacatc
      241 gttcgattgc atatacggca ccggtatcca tcccgctgtc catctgcatg atcgaaatac
      301 cggtttccga atctcccgcc agaatcgcgg cttggatcgg agacgctccg cgccaacgcg
      361 gcaataccga tgcgtgaatg tttatacatc cgtaccgcgg taaatccaat acttttttac
      421 ggagaatttt tccgaaagcc attacaacaa tcaaatccgg attccattcc tgaagggtct
      481 cgaaaacacc cggatctttc attttttccg gttgaatgac cggaatccct tcggcagctg
      541 ccgcaagttt taccgcgggc ggtttcggca atcgacctct gccggccggc cgatccggtt
      601 gggtgacaac cccgacaacg ggataaaatt gcgcaagtcc ccgtaaagtc ggaacggcga
      661 tttccggaga tcccataaaa acgattcggg gtgctcgttc gctcataaaa atcttccttc
      721 ccgtttttct ttttttctca caccgattat agggcttttt cggtttgttt gcttggattt
      781 gtgaataata tcattccgat ccaatatcat ttaaacaacc attacccgtc taggttccgg
      841 gtaatggttg tttgacgttc ttctttcaat tgaggagaaa tgaaaaaaag ttccgtttat
      901 tcttcggact taacactttc ctgcacgaca gcaccggttt cgccggtcga ttccgaatcc
      961 ggcaacacac aacgaaaacc gatataatag ctgctgttca tatttacgta actccggtta
     1021 gcggttctca gataagccaa atagttattg atttccgttt ccgcagccga tccgccgcgg
     1081 atcacatttg cgatcccgct ttgtgatccc ttcggattac gaatcacttc cgtacccgtt
     1141 tccgcttcat cgaccaatgt ttgataatag tccgcttgat actttgatga agtccattcc
     1201 catacgtttc cggccatatc catggcgccg taaggacttg ctccttccgg gaaagaaccc
     1261 actttgaaca gtccgcttac ctgttgtgca tcgggatcgg tataacccgg ataattcgca
     1321 taacgtgttc cctcgaccgg ctcttcgtca ccccaaggat aaattctgcc gtcggttcct
     1381 ctggctgctt tttcccattc gatttcggtc ggcaatcttt tcccggccca ttcgcaaaac
     1441 tgttcggctt cctcgacggt tacataagtc accggcatat ccgcatacgt cgggtcgttc
     1501 aacagcatat aagaagattg agtacatccc ccggcttgga cacactttag atatctcgca
     1561 ttcgtcactt cgtatttatc gatccaaaaa ccatccaaat aaacggtaat ctgcggagtt
     1621 tcttcgatcg tattggcata cccgtctttc aggggatcgg atcccaacag aaattctccg
     1681 gcttttacat ataccatttc agcctgatct ttttcggaaa cgatcggtcc tttgtcaacc
     1741 ggcgtcgggg taattgtcac ctgaacttcc gcagttccgg gagtcaatac cgtctgtgtt
     1801 ccaccgaaat cagcagcttc ggtcaccgct ccggctacgg gtacattcag atcaaacaac
     1861 gtcggaacaa cggtaacggt agatgtcatt gtcggcatcg gcgtcaacgt agcggtcggt
     1921 gtcgcagaaa gccgcgcaat cgcttcggct tgttgcgtct gatgaatgat tgccaaagtc
     1981 gcttgaaccg gatcaacggt agcggtggca gtcattctcg ggccgaaaag ggacggacga
     2041 ccttcgctgt cagagaaaaa ccagaatatt cccccgatca ccaaacagat cagtaaagct
     2101 gaaatgatat aagcggaagt cttttttgta ccttttttct ccgttttttc gggttgatgc
     2161 ctcggttttg atttcatcat taaatcaagt tctttggtct ctttatttaa gtcggtatcg
     2221 accggcaagg tgcccggctg cctcacgacc gtctgaatgg tgttttcatc atatatctta
     2281 atcgcaatcg cagaagcatc cgccggacta ttctgattgg cggcataagc caataaattt
     2341 cgaacgattt ctttcggggt gtcggcttga acagcttcta aaatctcatc cttaccgata
     2401 taagcggtca gtccgtcact gcacatcaac aagacatccc ccggttttac ttcgataccg
     2461 tcataaatat caacgacaat atctttttca cttcccaatg cccgtataat aggtttttga
     2521 gcatcggttt caaactcttc ttctttggtt aattcaccct ttcgaacttt ttcttcgaga
     2581 atattctgat cttcggtgat ttgatagact ttatcgttcc gcacgacata aacacggctg
     2641 tcgccgatcg tgccgataac ggcattccca tccgttatcg cagtggcaag cgcaaccgtt
     2701 gccatctttt cctgttttaa ttccgcataa gcatagattt cgttattggc attccgcatg
     2761 gctaacgcca acttattcgc atcgataaag tcggcgcttt tgaaatattc ataaaggatt
     2821 ttcttccctg cgaagcgggc tgtgatttcc ggatgaaacg ttcccgtcac accatccgtc
     2881 aggacataca gattcgaggc gcccaccgtt ccttcgggtt ccttttcaaa gaatataacg
     2941 aaatcttcat tgatttcttt atgaacgccg ggaatcgttc ccgcataacc ggatgagtag
     3001 atttttttct ccgcagtttt atcttccata aatatccttt gactcctgat tcattcctaa
     3061 aaatcgtccg cagaacttga atcctatcaa caatttaagt gcaagtttta tgccatgatc
     3121 gctacgatcg gacagattcc atatcacgcg atgcaatgac atcaataccc gtaccgagta
     3181 cattctttac aatcacatca cccatccgaa caggggcggt cacttctgtt tttcgtaatt
     3241 cagccgacag gtctttcaat cgatctttcg ggaaaggctg tgtaacggta accggcagta
     3301 ccggatgaac tccattacga attcggacgg tggaagcaaa catcc
//
LOCUS       DAOMIV010000202         3340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_30279_length_3340_cov_3.797260, whole genome shotgun sequence.
ACCESSION   DAOMIV010000202 DAOMIV010000000
VERSION     DAOMIV010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30279_length_3340_cov_3.797260"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..833)
                     /locus_tag="PKV59_05600"
     CDS             complement(183..833)
                     /locus_tag="PKV59_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46262.1"
                     /translation="MFKLIYNGEKEKKAGKKIFGETMQKIVEHDPLAVYLDGDIMNSI
                     GTLGLEKKFPDQVFECGIQEADMMGIAGGLSAVGKKPYVHTFAAFAARRAFDQVFISI
                     AYAKNNVRIIGSDPGVTSAYNGGTHMPFEDICLYRAIPHSTVLEFTDSAMLASLLPTL
                     KDRPGLTYCRLNRKEGTAIYSEDSTFEPGKANLLRDGKDVTIVACGMMVGESLKAA"
     gene            complement(840..1667)
                     /locus_tag="PKV59_05605"
     CDS             complement(840..1667)
                     /locus_tag="PKV59_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQP46263.1"
                     /translation="MITPLKEFATDIRIQEMIEFKARGFGHTGGSLSVADCLAALYGG
                     IMRIDPKNPQWEDRDRLVLSKGHAGPALYATLALKGYFPLEELKTLNQNGTKLPSHCD
                     RNLTVGVDMTTGSLGQGASTACGMALGLKHSGKDARVYLILGDGECQEGQVWEMALFA
                     AHHKLNNLIAFIDYNKRQLDGYVKDICDLGDLVAKFKSFNWYTTMIKGDDPDLIRQTV
                     LDVQKNQGDRPGMIVLDTIKGAGVPQIENIPLNHHTNVTPEEADEIIAQLEEFKRKG"
     gene            complement(1850..3112)
                     /gene="pgk"
                     /locus_tag="PKV59_05610"
     CDS             complement(1850..3112)
                     /gene="pgk"
                     /locus_tag="PKV59_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQP46264.1"
                     /translation="MEIKMLSLEGVDLRGKKVLLRPDINSPLDPQSKEIVNDERIVKT
                     IPVIQMLLKGGASVAIIAHQGDTLDYQNLTDLSKHAQKISEYLDHPVEYLDDVCGPAA
                     VQRVKALKPGEIVILGNLRYLTEEVSTFERDVKLDAAAYTKTWEVRNLAPLFDLYVNE
                     AFSAAHRNSPSMVAFQELLPTYAGPLLFQEYEALSKILHGAQKPAVFVLGGAKISDAF
                     GMMKPVLENGTADKILTSGVTGLVMLIAAGINVGEKTVRFLKDKDLLGFVEDSKEYLK
                     NYPDKIMMPVDVAIEENGKRVEIDIDEMPKDTLYLDIGAKTVAAYREVLMHAKTIFAN
                     GPAGVYENPLFEAGTKGLWQAIADSPAYSVIGGGDTISSATRLIDISKIGYVCTGGGA
                     MVRFMSGKKLPLISAMEKAYERDKSRTI"
     gene            complement(3103..>3340)
                     /locus_tag="PKV59_05615"
     CDS             complement(3103..>3340)
                     /locus_tag="PKV59_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46265.1"
                     /translation="FKYARDLGNPRGDMYEIGLWSDSIVESGDEIMFAINIPQESVTI
                     PETMDAVRAACEMQTDRLEGIGKTNTYLGMGKWK"
BASE COUNT          867 a          809 c          684 g          980 t
ORIGIN      
        1 caaggagtgg tttcagcgag gaaggcggca accgcagctc ctaaaccacc gacgatattt
       61 gcattttcag cggtaacgaa ggcatgagtc ttaccggcaa attctttgat aatctcttca
      121 tcgagcggtt tgatggtaaa catatcaatt acagcggctg aaatcccttc tttttccagt
      181 atttaggctg ctttcaacga ttcaccaacc atcataccgc aagccacgat tgtcacatct
      241 tttccgtctc gtaacaaatt tgcttttccg ggctcaaaag tggaatcttc ggaataaata
      301 gcggttcctt ctttccggtt taagcggcag taagttaaac cgggtctgtc ttttaatgtc
      361 ggaagaagag atgcaagcat agcgctgtcg gtgaattcaa gaaccgttga atgcggaatc
      421 gcgcgataaa ggcaaatatc ctcaaaaggc atatgggttc ccccgttata agctgaggta
      481 acgccgggat ccgaaccgat tattcttacg ttattttttg cgtatgcgat tgaaataaag
      541 acttgatcga aggctcgacg tgcggcaaaa gccgcaaagg tatgcacata aggtttcttt
      601 cctacagcgg aaagcccacc cgcaatcccc atcatatcag cttcttgaat tccgcattcg
      661 aaaacttgat ccgggaattt cttttccagg cctaaagtcc cgattgaatt catgatatcg
      721 ccatcaagat aaaccgccaa cggatcatgt tccacgattt tctgcatcgt ttcgccgaaa
      781 attttttttc cggctttttt ctctttttca ccattgtaaa ttagtttaaa catgacagct
      841 tatcctttcc tcttgaattc ttcaagctgg gcaattattt catccgcttc ttcgggagtg
      901 acattcgtat gatgatttaa tgggatattt tcgatttgag ggacacctgc tcctttaatc
      961 gtatcgagaa caatcattcc cgggcgatct ccctgattct tttgaacatc aagaacagtt
     1021 tgtcggatca aatccgggtc atctcctttg atcattgttg tataccaatt aaaactcttg
     1081 aattttgcca caagatcgcc caaatcgcag atatccttta cataaccgtc caattggcgc
     1141 ttgttataat cgatgaaagc gatcaggtta ttgagtttat ggtgggccgc aaaaagagcc
     1201 atttcccaca cctgcccctc ttgacactca ccgtcgccga ggattaaata tacacgagca
     1261 tcttttcccg agtgttttaa gccgagtgcc attccgcatg ctgtcgaagc accttgcccc
     1321 aaagaaccgg tggtcatatc gacaccgacc gttaaattac gatcacagtg gctgggaagt
     1381 tttgtcccgt tttggttaag agtctttaat tcttccaacg ggaaatagcc ttttagtgcc
     1441 aatgtcgcat aaagagccgg ccctgcatga cctttggaca ggaccaatcg atcacggtct
     1501 tcccattggg ggttcttcgg atcaattcgc ataatcccac cgtagagagc agcgagacaa
     1561 tcggcaacgg aaagtgaacc acccgtatgg ccgaaaccgc gggctttgaa ttcaatcatt
     1621 tcctgtattc tgatgtctgt tgcaaattct tttaagggag taatcatata atattgtttc
     1681 ctttcaatcc gctttcgttc tatttgtgca agatcaaaaa ttctgcaact tcctgctaat
     1741 tgtatcttat aagtctgacc gattatgata tttcccgaga aatccgcaca ttctcgggtt
     1801 atctttttct caaatattta taaagacaaa gaaccgttta tttcatcgct tagattgttc
     1861 tcgatttatc tctttcataa gccttttcca tcgcagagat caaaggtaat tttttcccgg
     1921 acataaatcg caccatggcg ccaccccctg tacacacata accgatcttc gagatatcga
     1981 tcaggcgggt tgccgaactg attgtatctc cgccgccgat gacggaataa gccggagagt
     2041 ccgcaatagc ctgccacaat cctttggttc cggcttcaaa taacggattt tcataaacgc
     2101 cagccgggcc atttgcaaaa atagtttttg cgtgcatcaa aacttcccga taggcagcga
     2161 ccgtctttgc gccgatatca agataaagcg tatctttcgg catttcgtcg atatcgatct
     2221 caacgcgttt cccgttttct tcgatagcca catcaacagg catcataatc ttatccggat
     2281 aattcttgag gtattctttt gaatcttcga cgaatcccag aagatcttta tccttcagaa
     2341 atcggacagt tttctctccg acatttattc ccgcggctat caacattacc aagccggtca
     2401 ctccggaagt caggatttta tctgcggtcc cgttttcaag cacaggcttc atcattccga
     2461 acgcatcgga gatttttgca ccgcccaata caaaaacagc gggtttttga gcaccatgaa
     2521 gaattttcga cagtgcttcg tattcctgaa aaagaagagg accggcatag gtcggaagta
     2581 attcctggaa tgcaaccata ctgggtgaat tccgatgggc tgcggaaaaa gcttcattca
     2641 catacaaatc aaacaacgga gcaaggttac ggacttccca ggttttggta tatgcggcgg
     2701 cgtccaattt aacgtctctt tcaaaagtag aaacctcttc cgtcagataa cgaagatttc
     2761 cgaggattac gatttcaccc ggtttcagtg ctttgactct ttggacagct gccggaccgc
     2821 agacgtcatc cagatattcc actggatgat caagatactc ggatattttc tgagcatgct
     2881 tcgatagatc ggttaaattc tgataatcca gggtatcacc ttgatgggcg atgatggcaa
     2941 cggaagcacc gccttttaac agcatttgaa ttaccggtat cgtttttacg atccgttcat
     3001 cattgacaat ttccttggat tgcggatcga gcggtgagtt gatgtccggt cgtaaaagaa
     3061 cttttttgcc tcgaagatct actccttcga gtgagagcat ttttatttcc attttcccat
     3121 tccgagatac gtatttgtct taccgatacc ttccaatcga tctgtttgca tttcgcaagc
     3181 agctcggaca gcatccatcg tttccggaat cgtgacagat tcctgtggga tattgattgc
     3241 aaacataatc tcgtcgcccg attcaacaat actgtctgac cataacccga tttcatacat
     3301 atcgccgcgc gggttgccga ggtcgcgggc atacttaaac
//
LOCUS       DAOMIV010000203         3339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_30294_length_3339_cov_3.953106, whole genome shotgun sequence.
ACCESSION   DAOMIV010000203 DAOMIV010000000
VERSION     DAOMIV010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30294_length_3339_cov_3.953106"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3119
                     /locus_tag="PKV59_05620"
     CDS             <1..3119
                     /locus_tag="PKV59_05620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HQP46266.1"
                     /translation="RAISVQPKRIATESSWDDRLPLRMEGQIPYDPTYGRYGTVLDLD
                     LFSSEEVLWKEKLIAKIDAADFLIIGSARGYGMADRAEKKFPILNSFYRQLVSCPDGD
                     PLTDCFNKLSVKSAKENPYFKPVAVFQSEPTLFGHSLSTQSAEEAFTVYDHPKVFIFQ
                     KQPQFSLSEFRKRLDFVNLSDISDKTPVEYKNMEQTYALLTESQAILQQSGGTWSELF
                     NRSSLINSNPILTVILWYLLILGIGLIFFPLSHMIFGSFRDKGYGISRFFGLLIFGYA
                     VWVLCFSILPYTKKTILSVFLVLAGVNLLIFLFNRKSILRDLYENRRYIFQSEIVFLS
                     CFVFFLLIRLGNPDLWHPYKGGEKPMDFSYFNAVIKSTSFPPYDPWFAGGSMNYYYYG
                     FFLSGLLVKWIGLIPSVAYNLILPTWYAFLGVGAFTLGSNLYAHRHFGNTEFNVEKGA
                     LKTGILSVLLLQVFGNLGTLKVIAVELTELGCAGINEASTAFEKIGCFFNGIGKILQG
                     QHFQMYAGDWYWIPSRAIPGEPITEFPFFTFLYGDPHAHLFALPITVMVLVWLTSLIV
                     RRKTSGNGFNLLSLASGALIIGSLIPTNTWDMPTYLLLSCIVLLDLGVQQPLFQFNKP
                     SSQTDSSLRKFSDQKIISSILSVVILCGLSFILFKPYLDTNTRESAIDVWNGSRTPVW
                     SYLMHWGLFLFALISWYYTETIDWLARVKVSDFRNFIQKQKNVLITVAVFFVGIMVAF
                     SAVKVWVAWIAVPLMFWTLCLLMRRDTPISQRFLLFLAGTALFITLFVEFFCLRGDIG
                     RMNMVFKLYLQAWVLFTPVSAFALISVIQRSFEKKTESEKSRKKVIWRFAYGFLLIGA
                     FSYTIFASVDKIKDRMSTVAPHTLDGMEFMKTSFYFQDGFLMDLSQDYDAIRWMQDNV
                     KGSPVIVEANATEYKWGNRFTVYTGLPGVVGWNYHQRQQRGNMSDQVWERVNAITSFY
                     NDSSLENAVDFLKRYNVRYIIVGQMERGMYAPNGIEKFVAGNGIDWDCVYLSRDTAIF
                     RVKEQEAK"
     gene            3128..>3339
                     /locus_tag="PKV59_05625"
     CDS             3128..>3339
                     /locus_tag="PKV59_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46267.1"
                     /translation="MVTNQIIQWLVVLIGIGIVGFPICFSMMRYLPDKGLAFSRIAFL
                     VLFGFFYWLACSLGIIGNNAGGILII"
BASE COUNT          846 a          647 c          766 g         1080 t
ORIGIN      
        1 aacgagctat atccgttcaa ccgaaacgaa ttgcaaccga aagcagctgg gatgaccggc
       61 ttcctttgcg gatggaagga cagattccct atgatccgac ttacggacgg tacggaaccg
      121 tgctggattt ggatctcttc tcatccgaag aagttttatg gaaagaaaag ttgattgcga
      181 aaatcgatgc ggcggacttt ttgattatcg gttccgctcg cggttatggg atggcggatc
      241 gggctgaaaa gaagtttccg atattgaatt ccttttatcg tcaactggta tcttgcccgg
      301 atggtgaccc tttaaccgat tgttttaata aattatccgt caaaagcgca aaagagaacc
      361 cgtactttaa accggttgcc gtatttcagt ctgaaccgac tctattcggt cactcattat
      421 ccacacaatc tgccgaagaa gcgttcacgg tgtatgatca tccgaaagta tttatctttc
      481 aaaaacagcc gcaattttct ttgtccgaat ttcggaaacg tttggatttt gtcaatcttt
      541 ccgatatttc cgacaaaact ccagtagaat acaaaaatat ggaacagaca tatgcgcttt
      601 taaccgaatc tcaagcaatc ttacagcaat ccggcgggac atggtccgaa ttgttcaacc
      661 gttcgtcact gattaattcc aatccgattt tgaccgttat tctttggtat ttattaattt
      721 tgggaatcgg attgattttt ttccctttat ctcatatgat cttcggttct tttcgggata
      781 aaggatacgg aatttcccgc ttctttggtt tattgatctt cggatatgcg gtatgggtgc
      841 tatgcttttc aatccttcca tatacaaaaa aaaccattct ttcggttttt ctggttcttg
      901 ccggagtcaa tctactgatc tttttattta atcggaaatc aatcttgcgc gatctttacg
      961 aaaatcggcg gtatattttt caatccgaaa tcgttttttt aagttgtttt gttttctttt
     1021 tattgattcg cctgggaaat ccggatttgt ggcaccccta taaagggggc gagaagccca
     1081 tggatttttc atacttcaac gccgtgataa aaagtacttc gttccctccg tatgatccgt
     1141 ggtttgccgg cggatcgatg aattattatt attatggttt tttcctgtca gggctgttgg
     1201 tgaaatggat cggattgatt ccgagtgttg cctacaatct gattctcccg acgtggtatg
     1261 catttttggg agttggagcc tttacgttgg gaagcaacct ttacgcgcat cgacatttcg
     1321 gaaataccga atttaatgtc gaaaaaggag ccctgaaaac cggcatttta tcggttttat
     1381 tattacaagt tttcgggaat ttagggacac tcaaagtgat cgcggtggaa ttgaccgagc
     1441 tgggatgtgc gggaattaat gaagcctcga cggcttttga gaaaatcggt tgctttttca
     1501 acggaatcgg aaagatcctt caagggcaac atttccaaat gtatgcgggg gattggtact
     1561 ggattccgag ccgtgccata ccgggcgaac cgattaccga atttcccttt ttcacttttc
     1621 tatacggaga tcctcacgcc catctttttg ccttaccgat cacggtcatg gtattggttt
     1681 ggttgacttc gctgattgtt cgcagaaaaa cgtccggcaa cggtttcaat cttttatctc
     1741 ttgcaagcgg cgcattgatc atcgggtcat taatcccgac caatacttgg gacatgccca
     1801 cttatttatt gctctcctgc attgtgttat tggatttggg agtgcagcaa ccgctttttc
     1861 aattcaataa accatcatca cagacagatt catcgctgcg gaaattctcc gatcaaaaaa
     1921 ttatttcttc gatcctatcg gtagtgattt tatgcgggct gtcttttatt ctgtttaaac
     1981 cctatttgga tacaaatact cgagaatccg cgatcgatgt atggaatggc agtcggacac
     2041 ccgtttggtc ttatttgatg cattggggat tattcctctt tgcactcatc agttggtatt
     2101 atacggaaac gattgattgg ttagctcgag taaaagtcag tgattttcga aattttattc
     2161 aaaaacaaaa aaatgttttg ataaccgttg cggtattttt tgtcggaata atggttgcat
     2221 tttccgcggt aaaagtatgg gtagcatgga ttgccgtccc gttgatgttc tggacgttat
     2281 gtttattaat gaggagggat actccgatca gccaacgctt cctgttattc cttgccggga
     2341 ccgctttgtt tatcacattg tttgtcgagt tcttctgtct cagaggggat atcggccgga
     2401 tgaacatggt attcaagctt tatttgcaag cttgggtatt atttacgccg gtatctgcat
     2461 ttgcgctgat cagtgtgatt caaaggagtt ttgaaaagaa gacggaatcc gagaaaagcc
     2521 ggaaaaaagt gatctggcgg tttgcttacg ggtttctatt aatcggcgcc ttcagttata
     2581 cgatttttgc ttccgttgat aaaatcaaag acagaatgtc aactgtggca ccccatacgt
     2641 tagacggtat ggaatttatg aaaacatctt tttattttca agatggtttt ttgatggatt
     2701 tgtctcagga ttatgatgct atccggtgga tgcaagataa cgtaaaagga tcgccggtaa
     2761 tcgtagaagc gaatgcgaca gaatataaat ggggcaatcg ttttacggta tacaccggct
     2821 tacccggagt cgttggttgg aattatcacc aacgccaaca gcgcggtaat atgtccgatc
     2881 aggtttggga acgggtaaat gcgatcactt ccttctataa tgattcatcg cttgaaaatg
     2941 cggtcgattt tttgaaacga tacaacgtca ggtatattat cgtcggacaa atggaacggg
     3001 gaatgtatgc tccgaatgga attgaaaagt ttgttgccgg aaacggaatt gactgggatt
     3061 gtgtttattt atccagagac accgcgattt ttcgggtaaa ggaacaggaa gcgaaatagt
     3121 cgatcatatg gttacaaatc aaataatcca atggcttgtc gtattaatcg ggatcggaat
     3181 cgtgggattt ccgatttgtt tttctatgat gcgatattta ccggacaaag ggttggcatt
     3241 cagccggatc gcttttttgg tactattcgg attcttttat tggttggcat gttcgttggg
     3301 catcatcggg aataatgcgg gcggaatcct cattatttt
//
LOCUS       DAOMIV010000204         3319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_30530_length_3319_cov_3.603554, whole genome shotgun sequence.
ACCESSION   DAOMIV010000204 DAOMIV010000000
VERSION     DAOMIV010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30530_length_3319_cov_3.603554"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKV59_05630"
     CDS             complement(<1..566)
                     /locus_tag="PKV59_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQP46268.1"
                     /translation="MKRSTVDDAYPNSSKRLFVPAVETTAEITVINSKFIATCAPVFS
                     VEEAKDFILKIRKTYPDATHHVPAYVIGHYSSTVCYCNDDGEPAGSAGRPILTVLTGS
                     GMGDIAMVVTRYFGGTKLGIGGLVHAYGGAAKKVLSIVPRAEKVLMDSFRFDIDYHLF
                     ELIKNLFNTENVLLIDHIFAEKITISAKV"
     gene            complement(587..1000)
                     /locus_tag="PKV59_05635"
     CDS             complement(587..1000)
                     /locus_tag="PKV59_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46269.1"
                     /translation="MSRVIRTGSASTERNRLKKALVLAIRELMKQSEPNETSRDLIAF
                     ILLCLERITETVEESAEAWEKRGYWLKADHFRMEWEWARLLAERIRPNVLTENYNKII
                     PDLIELSQSLNDVTISDNHRLGTPWLGAWDQLHKK"
     gene            1326..2672
                     /locus_tag="PKV59_05640"
     CDS             1326..2672
                     /locus_tag="PKV59_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HQP46270.1"
                     /translation="MTISEAMEEIKTLSSIPLELKYQKSLIQVNPEQFSFKIDVEKAQ
                     SELESKASEMCSSKIFLHSLFKEQKRDPISIALESNADEEKIESFLRNEISARYDQPA
                     AAKYPDLNGSGFYSGHPGYQLDIKKAVKMIQEALTSFDHQTIDLPIISIPEPPSSLTN
                     LEILLSSVVDQNQDSDQITEIYLLDPKTGEEFDFARQNKSNLIPEIAFTAASTIKIPI
                     MISAFKHLDEKPSAVVSRQLELMITESKNEQTDWLLENVIGGQTAPLEVTKDIRALGF
                     QNTFLAGYFYLGAPLLDRIETPANTRTDIDLKPDIYNQTTPKDIGLLLYLIYQCDLYG
                     KGKLTEVFPNEITQSECSDMMTLLKNNHLPYLISAGIPDGIPIAHKHGWIEESDGLLH
                     TMSNVAAVYSPGGDYILSIYTYHPQNLIFEKGNILFSRISSAVYGFFNPQQNDTVN"
     gene            complement(2686..>3319)
                     /locus_tag="PKV59_05645"
     CDS             complement(2686..>3319)
                     /locus_tag="PKV59_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQP46271.1"
                     /translation="LVDDHEVVRLGLKSLLEQGSRFEVVGEASNAKEAIEITGKLRPD
                     IVLMDIRLPGQSGIDACEEITQKYPDVKVVMLTSYAEDEMLFSAIRAGASGYLLKQIS
                     SEDLVRSLESVARGDALLDPLVTQRVFQEVRRAVKEEEASAFAILSQQEKHVLILVSE
                     GKTNREIAKSLFLGEGTVRNYVSSILSKLGTSNRAEAAAYAVEHNLKDYL"
BASE COUNT          976 a          732 c          622 g          989 t
ORIGIN      
        1 actttggcag atatggtaat cttctccgca aaaatatgat caatcaataa aacattttcc
       61 gtgttaaaaa gattttttat cagctcaaac agatgataat cgatatcaaa tcgaaaagaa
      121 tccattaata ctttttcagc acgggggacg attgataata cctttttggc ggcaccgcca
      181 taagcatgaa ccaatcctcc gataccgagt ttcgttcctc cgaaataacg ggtaacaacc
      241 atagcgatat cacccattcc gcttcccgtg agaacggtca aaatcggcct tcccgccgaa
      301 ccggcgggtt caccgtcatc attacagtaa caaacagtcg aagaataatg gcctattaca
      361 tacgcaggta catggtgcgt agcatctgga taggtcttgc gtattttcaa aataaaatct
      421 tttgcttctt caaccgaaaa tacgggcgca caggtggcta taaattttga attgataacc
      481 gttatttcag ctgtggtttc gaccgcaggg acgaaaagcc gttttgagct gttcggatat
      541 gcatcatcta ccgtgcttct tttcatggag ataaattaat tgagtctcat tttttatgga
      601 gttggtccca tgctccaagc cagggagttc ctaaccggtg attatcggat atcgtaacat
      661 catttagtga ttgactcaat tcgatgagat cgggaattat tttgttgtaa ttttctgtca
      721 atacattcgg gcggatccgt tccgccaaca aacgcgccca ctcccattcc attcgaaaat
      781 gatcagcttt taaccaatat ccgcgctttt cccatgcttc cgccgattct tcaaccgttt
      841 cagtgattcg ttccagacac aacaaaataa aagcaatcag gtctctggaa gtttcgttcg
      901 gttccgattg cttcatcaac tctcggatcg ccagaaccag agcctttttt aatctgtttc
      961 tttcagtact tgcacttccg gtacgaatta ctctgctcat aaaatatctt tataccccat
     1021 gtttttattt tctgggatcg aattatacag tataagtatt gatcactcca tataagaata
     1081 ttttgtctct ataacctccg cgtattctct tcaatttcac aaaatcaaaa tgtccggcca
     1141 atttcctgat aaaatggaac aggttaaaac gtatgtcccg aaaaattcgc tatggttttt
     1201 ttgcaattgc cttttctctc ctttttttat cggttgttct tttgattatt tcaagagata
     1261 tattttatgc tcaatgcaat attcttcctg aaaattcaac agctgggccg ttgaatatcg
     1321 gaggtttgac gatctcagag gctatggaag aaataaaaac gctttcatcg attccgctcg
     1381 aattaaaata tcaaaaaagt ttgattcagg ttaatccgga acagttttct ttcaaaattg
     1441 acgtcgaaaa agcacaatcc gaattggaat caaaagcatc cgagatgtgt tcgtcaaaaa
     1501 tatttttaca cagtttgttc aaggaacaaa aaagagatcc aatctcgata gcactggaat
     1561 ccaacgcaga tgaagaaaaa atcgagtcat ttttgcgaaa cgagataagc gctcgatatg
     1621 atcaaccggc agcggcaaaa tatccggatc ttaacggttc cggtttttat tcggggcatc
     1681 ccggttatca attggatatc aaaaaagccg ttaaaatgat tcaagaagcc ttaacttcat
     1741 ttgatcatca gacgattgat ttaccgatta tcagtattcc cgagccgcca tcttctttaa
     1801 caaatttgga aattttatta tcctcggttg tagatcaaaa tcaagactcg gatcaaatca
     1861 ccgaaatata cttattagac cccaagaccg gcgaagaatt tgattttgcc cgccagaata
     1921 aatcaaattt aattccggaa attgctttta ccgctgccag taccataaaa atcccgatta
     1981 tgatatccgc ttttaaacac ttggatgaga aaccgagtgc cgttgtttcc cgacagttgg
     2041 aattgatgat aaccgaatca aaaaatgaac agacagattg gctcttggaa aacgtaatcg
     2101 gtggccagac agccccgtta gaagttacaa aagatatcag ggcgctcggg tttcaaaata
     2161 catttcttgc cgggtatttt tatttaggtg cgcctttgct tgatcgaata gaaactccgg
     2221 caaatacacg aacagatatc gacctaaagc cggatatcta taaccaaaca acgcccaaag
     2281 atatcggttt gttgctgtat ttgatttatc aatgtgattt atatggtaag gggaagctga
     2341 cggaggtttt tccgaacgaa atcacgcaaa gcgaatgcag tgatatgatg acattgctca
     2401 agaacaatca tttgccatac ttgatatcgg cggggatacc ggacggtatc ccgatagcac
     2461 ataagcacgg atggatagag gaatccgacg gactcctcca taccatgagc aatgtggctg
     2521 cggtctacag tccgggcggt gattatattc taagtatcta tacctaccat ccgcaaaatt
     2581 tgatttttga aaaaggtaat attttgttct ctcgtatttc ttcggcagta tatggatttt
     2641 ttaatccgca acaaaacgac actgtcaatt aaccaagctt ccgttttaca gataatcttt
     2701 caaattatgt tcgaccgcat aagcagccgc ttcggctcgg ttgctcgtgc ctaatttgga
     2761 taagatactg ctgacataat ttcgaacagt tccttctcct aagaataaag actttgctat
     2821 ttcccgattc gtctttcctt ccgaaacaag aatcaacaca tgtttttcct gttgacttaa
     2881 tatcgcaaat gccgatgctt cttcttcttt taccgctcgg cgaacctcct gaaagacacg
     2941 ttgtgtcaca agcggatcca aaagagcatc tcctctggct acagattcaa gagatcgaac
     3001 caaatcttca ctgctgattt gttttaacag gtatccagaa gctccggctc ttattgcaga
     3061 aaaaagcatc tcatcttcgg cataagatgt cagcatgaca actttgacat ccggatattt
     3121 ttgcgtaatt tcttcacagg cgtcaatacc ggattgcccg ggaagccgaa tatccattaa
     3181 aacaatatcc ggtcgcaatt ttccggtgat ttcgatcgct tcttttgcat tgctcgcttc
     3241 cccgaccacc tcgaatcgcg atccctgttc aagaagagat ttaagaccaa gtctgacaac
     3301 ttcatgatca tctacgaga
//
LOCUS       DAOMIV010000205         3299 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_30798_length_3299_cov_3.782367, whole genome shotgun sequence.
ACCESSION   DAOMIV010000205 DAOMIV010000000
VERSION     DAOMIV010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30798_length_3299_cov_3.782367"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1265
                     /locus_tag="PKV59_05650"
     CDS             <1..1265
                     /locus_tag="PKV59_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP46272.1"
                     /translation="ILLIPQYTVWENVILGMEPLGKFNRIHRDLARKQVQDRIDEFQF
                     NLDPDAKVEDLSVAARQKVEILKLLYRDVTFLTLDEPTAVLTPQEIPQLFDELRRLRN
                     NGHTIAFISHHLDEVLELSDRISVLRKGKKVATIPASQTSESLLAEMMVGREVVFESI
                     KETVRPGKLIFRLQNIRLQSPNKKKLLDDVNIEVKAGEIVGIAGVEGNGQLELVEVIT
                     GLATPDQGQLLINGVNLTKASILERRKYMAFVPQDRALMGSCLNASIKENLIMTHYKL
                     NPEFTRHGHILNDKKSTEFAYEIREKFDIQLHSIEDEMKSLSGGNQQKVILGRELLLS
                     APFILLDQPTRGLDVGSIEYVHQQIIRMSKENRAILLLSSDLEELFRITDRILVLHRG
                     QIVANLITNETNINEVGYLMLEGAAHGA"
     gene            1255..2301
                     /locus_tag="PKV59_05655"
     CDS             1255..2301
                     /locus_tag="PKV59_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46273.1"
                     /translation="MAHNLRNILINLAGILIAFLLGAVVMIFQGYNPLETYEALFGYS
                     LGNTYSFMTVLKNSVPLILTGLSASVAFASGPVNLGQPGQLLMGALFATVGGLYVNLP
                     SVLMIPFLLVSAMVGGALWSGIAGLLKRLFNMDEYITTLMMNMIADFFTYWAITYPFF
                     EKNANHPQTPRITKNGWMPEIGDFNTNFLVTLIVFVIIWFIFYRTTNGYEWRISGKNS
                     LFARLGGVEIEKNYMHVMLLTGALAGLAGGLIIMAGPHRFIKGLGANYAWDGIMVSMV
                     ANNGIVATLIYGIFFSILQTGALGMELITQVPSELITVLQAVIVLVVVASRGYFTLLL
                     SKIMTKRKLKERSA"
     gene            2298..3197
                     /locus_tag="PKV59_05660"
     CDS             2298..3197
                     /locus_tag="PKV59_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46274.1"
                     /translation="MTIFIGLINGMISAATPILLSALGGALTYYAGIFNIAMEGMMLS
                     GAFFAVLGSYYFGNWLIGMLFGILGALLIAGIFILFSVVLKVDEFVTGVGLNMFSVGL
                     TTYLLRQIFKVKGAFTDPGIYSVPKIRLPFIEKIPFVGDIISDQNLIVWIAFIATLLV
                     NYFVFKTQFGLRLRAAGYNRICLDTSGVSSNKIKTVSLLLCAVLCGFAGTYLSLGYVT
                     LFSENMSAGRGWISLAAVILVSGNPIGIALVSLLFGLADGLGLYLQHIFPSQFTSMLP
                     YIATLAALFFYSVNNKKKMEVKL"
BASE COUNT          895 a          640 c          744 g         1020 t
ORIGIN      
        1 aaattttatt aattccgcaa tataccgttt gggaaaatgt gatattgggg atggaaccgc
       61 tcggtaaatt taatcgtatc catcgtgatc tcgcacgaaa acaagttcaa gatcgtatcg
      121 atgaatttca atttaatttg gacccggatg cgaaagttga agatctttct gtcgctgcca
      181 gacaaaaagt cgaaatactg aaactcctct atcgggacgt cacttttttg actttggatg
      241 aaccgaccgc ggtattgact cctcaggaaa ttccgcaatt gttcgatgaa cttcgcaggc
      301 ttcgaaataa cggccacacg atcgctttta tttcccatca tttggatgaa gttctcgaac
      361 tcagcgatcg gatctctgtt ctgcgcaaag ggaaaaaagt agctacgatt ccagcgagtc
      421 aaacatcgga atctttactg gcggaaatga tggtgggacg cgaagttgtt tttgagtcta
      481 ttaaagaaac agtccgtccg ggaaaattaa tatttcgttt gcaaaatatc cgtttgcaat
      541 ctcccaataa aaagaaactt ttggatgatg tcaatatcga ggttaaagcc ggcgaaatcg
      601 tcggtatagc cggagtggaa ggaaacggcc agctcgaatt ggttgaagtc ataacgggat
      661 tggcgacgcc ggatcaggga cagcttctga taaacggagt gaatttgaca aaggcttcta
      721 ttttggaacg cagaaaatat atggcattcg ttccgcaaga tcgggccttg atgggctcat
      781 gtctgaatgc atccatcaaa gaaaatctta ttatgaccca ctataaactg aaccctgaat
      841 ttacacgtca cggacatata ttaaatgata aaaaatcgac agaatttgct tatgaaatcc
      901 gggaaaaatt cgatatccaa ctccattcta tcgaagatga aatgaaatca ttatcgggtg
      961 gaaaccaaca gaaagtcatc ttagggagag agctgctcct ttccgcaccc tttattttgc
     1021 ttgaccaacc gactcgcggc ctcgatgtcg gttcgattga atatgttcat caacagataa
     1081 taaggatgag caaggagaat cgagcaatat tactcctttc atcggatctc gaagaattgt
     1141 ttagaataac agaccgcatt ctggtcttgc accggggaca aattgtcgca aacttgatta
     1201 caaatgaaac caatattaac gaagtcggat atctaatgtt ggaaggagcc gctcatggcg
     1261 cataatcttc ggaatattct aatcaatctt gccggtattc tgattgcatt cttattggga
     1321 gctgtcgtca tgatctttca gggatataac cccttggaaa catatgaagc tctttttggt
     1381 tattctctcg gaaataccta ttcctttatg acagttttga aaaattcggt ccctctgatt
     1441 ctgaccggat tatccgcttc ggtggccttt gcttccggtc cggtaaatct cggacagccc
     1501 gggcaattgt tgatgggtgc cttatttgca acggtcggcg gtctttatgt caatctccct
     1561 tcagtattaa tgattccttt tttgttggtg tcagccatgg taggaggagc actttggtcg
     1621 gggattgcgg gtttgctcaa acggttgttc aacatggacg aatatattac gacattgatg
     1681 atgaatatga ttgcggactt ttttacatat tgggcgatca cctatccttt ttttgaaaaa
     1741 aatgccaatc accctcaaac gccgcgaatc acaaaaaacg gctggatgcc tgaaatcggt
     1801 gactttaata cgaattttct tgtgacatta atcgttttcg tcattatatg gtttattttt
     1861 tatcggacta ccaacggata tgaatggcgc atttccggta aaaacagcct ttttgcacgc
     1921 cttggcggtg ttgagatcga aaaaaactat atgcacgtaa tgctattaac cggtgcgtta
     1981 gccggattgg ccggcggttt aattattatg gcagggcctc atcgatttat caaaggattg
     2041 ggagccaatt acgcttggga tggaattatg gtttccatgg tcgccaataa cggtattgtc
     2101 gcaacattga tatacggtat cttcttctcc attttgcaaa cgggtgcgct cggaatggaa
     2161 ttaattacac aagtaccgag tgaattaatt acggttttgc aagcggtcat tgttttggtt
     2221 gttgtcgcca gcagagggta ttttacattg ctgctttcaa aaataatgac caaacgaaaa
     2281 ctgaaagaga ggtctgcatg acgattttta tcggattgat taatggaatg atttcagcgg
     2341 caacgccaat cctcttatcg gctttgggag gcgcgctgac ttattatgcc gggatattta
     2401 atattgcaat ggaaggaatg atgctcagcg gcgctttttt tgccgtattg ggatcatatt
     2461 attttgggaa ttggctcatc ggcatgctct tcggtatctt gggtgctttg ctgattgccg
     2521 gtatctttat tctgttttca gtcgtcttga aagtggatga attcgtaaca ggcgtcggtc
     2581 taaatatgtt ttccgtcgga ttgacaacct atctgcttcg tcagattttt aaagtgaaag
     2641 gtgcttttac cgatccggga atttactctg ttccgaaaat cagactccct ttcatagaaa
     2701 aaataccgtt tgtcggagat attatcagtg atcaaaatct tattgtctgg atagcattca
     2761 tagcaaccct attggtaaat tattttgtat ttaaaactca gtttgggctt cgtttaagag
     2821 ccgcaggata taatcggatc tgtttggata ccagcggcgt ttcttccaac aagattaaaa
     2881 cggtttcatt attattatgt gccgttttat gcggtttcgc ggggacttac ctttctttgg
     2941 gatatgtaac attattcagc gaaaatatgt ctgcgggtcg cggatggatt tcactcgctg
     3001 ccgttatatt agtttcgggc aatccgatcg gaatcgcttt ggtttctctc cttttcggtc
     3061 ttgccgacgg attgggtttg tatttgcagc atattttccc atcgcaattc acttcgatgt
     3121 tgccatatat tgcgacgctt gcggcactat ttttctattc tgttaataat aagaaaaaaa
     3181 tggaggttaa gttatgaaag cttgggaaat ggaacgcgaa atacgcgata acgccgtccc
     3241 taaggacaga agaaagaatg aatctcactg gtataaatcg gatatgacga ttccctatg
//
LOCUS       DAOMIV010000206         3288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_30938_length_3288_cov_3.727807, whole genome shotgun sequence.
ACCESSION   DAOMIV010000206 DAOMIV010000000
VERSION     DAOMIV010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30938_length_3288_cov_3.727807"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="PKV59_05665"
     CDS             complement(<1..279)
                     /locus_tag="PKV59_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46275.1"
                     /translation="MNFQQNIVCPDYNHSNLGIISSVLNYYAVPSSTKSSTDVDSLLV
                     QKHPRNVIFLLIDAMGTGILNRHLPEDSFLRSHRRGDLTAVYPCTTVCV"
     gene            complement(289..1065)
                     /locus_tag="PKV59_05670"
     CDS             complement(289..1065)
                     /locus_tag="PKV59_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HQP46276.1"
                     /translation="MPDITILQILILSGFAFLAGLIDSVAGGGGLIQLPAIMAILPYA
                     PVASLLGTNKLSSSCGTGMAVFQYSRKVPIEWKKVLPIAGVAFCSSIFGAMTAVSIPN
                     QWMRPLVLVLLTVVLIYTILNKNLGVRVHEVSLSFRQQLGLSLLAGLVIGFYDGFFGP
                     GTGNFLILALIGIFGMDFLRASASCKIINLATNFGALILFGSQGAIFAYLGLIMAVFN
                     MAGSYVGVRLAVLKGSKFIRILFIVVVSGLLLKQCIDYLL"
     gene            1278..2342
                     /gene="thrC"
                     /locus_tag="PKV59_05675"
     CDS             1278..2342
                     /gene="thrC"
                     /locus_tag="PKV59_05675"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQP46277.1"
                     /translation="MSYQGIIHRYHEFMDLPPAVQPITLYEGNTPLIPVPAIADNLGG
                     GFELYVKYEGLNPTGSFKDRGMTTAMTMAKHNGKKTVICASTGNTAASAAAYASRAGL
                     RAIVMIPEGKVAAGKLAGAVAYGAEIIQIPGSFDDALSFVVEISQKSEIELVNSLNPY
                     RMEGQKTASFEICDELGSAPDWLCLPVGNAGNITAYWMGFKQYNRIKNTGLPHILGVQ
                     AEGAAPLVIGHTVEHPETVATAIRIGNPARGEQALMAAEESKGRIIAVSDEKILAAQK
                     LLASSGIWVEPASAAGLAGLTDRIKNNSFDPKGKRIVLVCTGHGMKDPDIIISRGIKP
                     VRLPVDFDKVAEYILGGRIQ"
     gene            2339..3250
                     /gene="thrB"
                     /locus_tag="PKV59_05680"
     CDS             2339..3250
                     /gene="thrB"
                     /locus_tag="PKV59_05680"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HQP46278.1"
                     /translation="MRSFAVYVPASTSNIGPGFDCVGLAVNLWNEARFYFDSSLSSIE
                     ITAEGYQSKIQKQPSENLIFRSFLHFFELKGIKVPEGLRIHCVNRVPIGSGLGSSSTA
                     ILIGLLAADELLGTKTSQQGILEIAASLEGHPDNVTPAIFGGLTAASMDETGVRTERF
                     PIADWNMAVVVPHFNLSTKAARKALPDTVPFRDAVFNLNHAIFLMRALQDGDEEELRF
                     AMQDRLHQQYRMRLIPGSEAILQAGVDAGAAATGLSGAGPGVIAFSMGKTDQILAAML
                     QAAHLAGYEADGFELKTSSNGAWIMPL"
BASE COUNT          939 a          792 c          792 g          765 t
ORIGIN      
        1 cacacaaacc gtcgtgcaag ggtaaacagc cgtcaaatcc ccccgccggt ggctccgtaa
       61 aaaactgtct tccggcaaat gacggttaag aatgccggtc cccatagcat caatcaaaag
      121 aaaaataaca ttgcgcgggt gcttttgaac gagcaaagaa tccacatccg tggaagattt
      181 tgtcgaagaa ggtacagcat agtaatttaa aaccgatgaa ataatcccta aattggaatg
      241 gttataatcc gggcaaacaa tattttgttg aaaattcatt tcctttcctt aaagtaaata
      301 atcgatacat tgtttcagca acaatccgct gaccaccacg ataaataaaa tgcgaataaa
      361 cttgcttccc ttaagaaccg caagccgcac tccgacataa cttcccgcca tattgaatac
      421 agccataatc aaaccgaggt aagcaaaaat cgctccctgt gacccgaaaa ggatcaatgc
      481 cccgaagttg gtagccagat tgataatttt acaggatgcc gaagcccgaa gaaaatccat
      541 cccgaaaatt ccgatcaacg caaggatcag gaaattcccc gtcccgggac caaaaaatcc
      601 gtcataaaag ccgataacaa gacccgccag caggctcaat ccgagttgtt gcctgaaaga
      661 taaagaaact tcgtgtactc tcaccccgag gttcttattc aatatcgtat aaatcaggac
      721 aaccgtcaac aaaaccaaga ccaacggacg catccattga ttggggatcg aaaccgccgt
      781 catggcaccg aaaatcgatg aacaaaatgc gacgccggcg atgggaagca ctttcttcca
      841 ctcgatagga acttttctcg aatattgaaa aacagccatt ccggtcccgc agctggatga
      901 aagtttattt gtaccgagca gggaagcgac cggtgcataa gggagtatgg ccatgattgc
      961 cgggagttga attagtcctc ccccgcccgc aaccgaatcg atcaaaccgg caagaaaagc
     1021 aaatccagaa aggattaata tttgaagaat tgtaatatcc ggcatggaat tgattccaat
     1081 ctcctgagag caatttaacc ttttcgatta taccctcagt cccgtcgttt tcccgtcgcc
     1141 accctttttt tcattcattt cggcgcaaaa ttcaaaaaat gtaaaaatca ccgcgaaaaa
     1201 acacgaaccc ttttcccaat ccgattgaag tagattagaa ttacacgcat aaccattttt
     1261 gcgtcggaga aacagacatg agctatcagg gcatcattca tcgctatcac gaattcatgg
     1321 atcttccgcc tgcggtgcaa ccgatcactt tatacgaagg gaatacccct ttgattcccg
     1381 tgccggcgat cgcagacaat ttaggcggcg gtttcgagct gtatgtcaaa tatgaaggcc
     1441 tgaatccgac gggttcgttc aaagatcgcg gaatgacaac cgccatgacg atggcgaagc
     1501 ataatggtaa gaaaacggtt atctgcgctt ccaccggaaa cacagccgcc tcggcagccg
     1561 cctatgcgag ccgagccggt cttcgtgcca tcgtcatgat cccggaagga aaagtcgccg
     1621 ccggaaaact ggccggtgcg gttgcttacg gagcggagat tatccagatt cccggctcat
     1681 tcgatgacgc gctgtctttt gtcgtagaaa tcagtcaaaa aagtgaaatc gaactggtca
     1741 acagtcttaa tccttatcgc atggaaggcc aaaagaccgc ttcttttgaa atttgtgacg
     1801 agctgggaag cgccccggac tggttatgtc tgccggtggg caacgctgga aatatcacgg
     1861 catattggat gggatttaaa cagtataacc ggataaaaaa taccggcctg ccgcatattc
     1921 tgggtgtcca agccgaaggt gcggcgccgc ttgtgatcgg acatacggta gagcatccgg
     1981 aaacagtggc gacggcgatc cgaatcggca atcccgcccg cggagagcaa gctttaatgg
     2041 ccgcggaaga atcaaaggga cggattatcg ccgtttccga tgaaaagatt ttggcggctc
     2101 aaaaattgtt ggcttcatcc ggtatctggg tggaaccggc ttcggctgcc ggtctggccg
     2161 gattaaccga ccgaattaaa aacaattcct tcgatcccaa aggcaaacga attgtgcttg
     2221 tctgtaccgg acacggtatg aaagatcccg atatcattat ttcccgtggg attaaacccg
     2281 ttcgtctgcc ggttgatttc gataaagtgg cagaatatat cctcggagga agaatccaat
     2341 gagatctttt gccgtttatg ttccagcatc caccagtaat attgggcctg ggtttgattg
     2401 tgtcggattg gcggttaacc tgtggaacga agcacggttt tattttgatt cctcgctgtc
     2461 atcgatcgaa attacagcgg aaggctacca aagcaaaatc caaaaacaac cgagtgaaaa
     2521 tctgatcttt cgttcgtttc ttcatttttt tgaattaaaa ggaattaagg ttccggaagg
     2581 actcagaatt cattgtgtga accgtgtgcc gatcgggtcg gggttgggct ccagttcaac
     2641 cgcgattctg atagggttgc tcgctgccga tgagctgctg gggacaaaga cttcacaaca
     2701 gggaattctt gaaatcgccg caagcctcga aggacatccc gataatgtta cgccggctat
     2761 ttttggcggt ctgaccgctg cttcgatgga cgaaacgggt gttcggacag aacgcttccc
     2821 gatcgccgat tggaatatgg cggttgtggt tccccatttc aacctgtcga caaaagcggc
     2881 ccggaaagct ttaccggata cggtcccgtt ccgggatgcg gtttttaatc tgaaccatgc
     2941 aatcttttta atgcgggcac ttcaggatgg ggatgaagaa gaattgcgat ttgccatgca
     3001 agatcgattg catcagcagt atcgaatgcg attaatcccg ggatccgaag cgattttaca
     3061 agccggagtc gacgccggtg cggcagccac agggttatcg ggagccggac cgggggtcat
     3121 tgccttcagc atgggaaaaa cagatcaaat cctggcagca atgctccagg cggcacacct
     3181 tgccggctat gaagcggacg gtttcgaatt aaagacctca tccaacggag catggataat
     3241 gcctctctaa aagaaggagg agacatgaaa aagaaattaa ttattatt
//
LOCUS       DAOMIV010000207         3287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_30956_length_3287_cov_2.940285, whole genome shotgun sequence.
ACCESSION   DAOMIV010000207 DAOMIV010000000
VERSION     DAOMIV010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30956_length_3287_cov_2.940285"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..566)
                     /locus_tag="PKV59_05685"
     CDS             complement(123..566)
                     /locus_tag="PKV59_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46279.1"
                     /translation="MNNINEGGFAKPFIKPVLSTKFHIDFDWWKENDSSWRLSLMGYH
                     CDKHKKFFESSNDNQLVDSVNSETGEIIQVDQLQDILINHCAKNDDFISGNSPLTDSV
                     FRIFLSNGNTPLDSEEISKIINRPAETILKTLSGPRVYKGIRPIL"
     gene            complement(559..2163)
                     /locus_tag="PKV59_05690"
     CDS             complement(559..2163)
                     /locus_tag="PKV59_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46280.1"
                     /translation="MNDKSVNAIVNWKDLNLSQSDLDFIYNYLLEKEVPMTSLELSKA
                     IIDEHIRLFRKELQKKEKSRGQVYRPNRDYQMGEEILFPLSDNQKAKVINIRQGNNPD
                     LPDFNAITFLFEDGTEKEFAGSLQDHRLNSFDYYSSDSEFLLDPEEVLDRFGGSISKK
                     VRQLLAQSDGLFTLADFWFPKALLSEVNLGYLNLAEAALEMEEGQPVQTRKILEAIEY
                     PMDSNDLLTEFSFNYALYKDDRFDEVGPTGKVLWVLKLLEPEDVRKKPLTLKYYGIDS
                     EKADINGYSLDHLQIHDELDPDNQIAPETEIKTFDICINYPHWKAGSLPLIGSIRPIF
                     PTALETDKVIFEFVDKNKSSKFPGWVLVKENYVSGLKQWYRENHIIPGSFIRLRKIDN
                     STDVEISMIQPRSSKDWIRTALFDSKGKIHFEIRQQPINTDFDERMCIYVENNPQLDL
                     IWDQNNKPDINFGKLVENIFKEISSDNPQGIVHFLELYAAVNMLHRCPPKPLLATLIH
                     NDHFEILDNYYFKINSKESKLETEYE"
     gene            complement(2208..2693)
                     /locus_tag="PKV59_05695"
     CDS             complement(2208..2693)
                     /locus_tag="PKV59_05695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQP46281.1"
                     /translation="MEDHSNEVDQATLAGITQTSPIKSWGDRMSYPVDEPCLVSGFGN
                     RRTYNDGSYTNFHSGVDFGVCTANNINIYAAADGTVLFSGDLPIHGKHTIIDHGWGVY
                     STYSHQSETFVTAGQQVKRGDLIGLIGSTGRSVGPHLHWEIKVNGIYVNPLAWINNSF
                     P"
     gene            complement(2767..>3287)
                     /locus_tag="PKV59_05700"
     CDS             complement(2767..>3287)
                     /locus_tag="PKV59_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQP46282.1"
                     /translation="VSFHGRLFFALAMERWNLHRRIALLVIRALGESPERIILGFMAS
                     TAALSMWISNTATAMMMTPIGLAVIYQTSEMLEHGSDIREAVRTGKFQFGAALMLGIA
                     YAASIGGIATIIGTPPNAVFAAILEEMYGVEIGFARWMLYGVPLALLFLLLTWLYLTR
                     IAFRFEFERRIL"
BASE COUNT          975 a          715 c          641 g          956 t
ORIGIN      
        1 aaattataac tcatgaaaag aacaaatttc agtttatgaa aaaaataagg gaaagcatct
       61 tggttttcct cttattacaa ttattcttca ttaaaaagcg aaataataaa ggggaagata
      121 aattatagga ttgggcgaat acccttatag acacgcggac cgctcaaagt ttttagaatt
      181 gtctcagcgg ggcggtttat gattttactg atttcttccg aatctaacgg tgtatttcca
      241 ttcgaaagaa atattcgaaa taccgaatcg gttaatgggc tgtttccgga aataaaatcg
      301 tcattttttg cacaatgatt tatgagaata tcttggagtt gatcaacttg gatgatttca
      361 ccggtttcag aattaactga atcgacaagt tgattatcat tagaagattc aaaaaatttt
      421 ttgtgcttat cgcagtgata tcccatcagg gataaacgcc aactgctgtc gttttccttc
      481 caccaatcaa aatcaatatg aaatttggta gaaaggactg gttttatgaa cggtttagca
      541 aaaccgcctt cgttgatatt attcatattc tgtctctaat ttggattctt tcgaattaat
      601 tttaaaataa taattatcaa gtatttcaaa atgatcatta tgtatcagag tcgcgaggag
      661 cggctttgga gggcagcggt gcagcatatt tacggcagca taaagctcaa ggaaatggac
      721 gataccctgt gggttatcac tcgatatttc cttgaagata ttttcgacca atttcccgaa
      781 attaatatcc ggtttgttgt tttggtccca aatcaagtcc aactgaggat tattttccac
      841 ataaatacac attctttcat caaaatcagt attgatcggt tgttgcctta tttcaaaatg
      901 aatttttcct tttgaatcaa acaaagcggt tctgatccag tctttcgacg atcgaggttg
      961 aatcatcgag atttccacat cggtcgagtt atcgatcttc cgcaatcgta taaaactgcc
     1021 gggtattata tgattttccc gataccattg tttaagcccg gaaacataat tctctttcac
     1081 taatacccac cccgggaact ttgaagattt gttcttgtca acgaattcaa aaatcacctt
     1141 atcggtttct aaagcagtcg ggaaaatagg acgaatactt ccgataagag gaagtgaacc
     1201 ggctttccaa tgaggataat tgatacagat atcaaaagtt ttgatttccg tttccggagc
     1261 gatttgatta tcaggatcta attcatcatg aatttgtaaa tgatctaaag aataaccgtt
     1321 gatatccgct ttttcactgt caattccata atattttaaa gtcagtggtt tttttctgac
     1381 atcttcgggt tccagcaatt ttaaaaccca taaaactttt cctgtcggac cgacctcatc
     1441 aaagcgatca tctttataaa gggcataatt aaaagaaaat tcggtgagca aatcattact
     1501 gtccatcgga tattcgattg cttcgagaat ttttcttgtc tgaacaggct gaccttcttc
     1561 catttcaaga gcagcttcgg caagatttaa gtatccgaga ttaacctccg atagaagcgc
     1621 tttgggaaac caaaaatcag ctaaagtaaa taatccatct gattgagcga gcaactgccg
     1681 gactttttta gaaatcgacc cgccaaatcg atcgagaact tcctccgggt caagcaaaaa
     1741 ttcggaatcc gaagaataat aatcgaatga attcaacctg tggtcttgca aactaccggc
     1801 aaattctttt tctgtcccat cctcaaaaag gaatgtgatt gcattaaaat caggaagatc
     1861 cggattatta ccttgccgga tattgataac ttttgctttt tgattatccg ataaaggaaa
     1921 gagtatctcc tcgcccattt gataatcccg gttcggcctg taaacctgcc cgcgagattt
     1981 ttcttttttc tggagctctt ttctaaataa tcgtatatgt tcgtcaatta tcgctttcga
     2041 taattccaac gaagtcatcg gaacttcttt ttccagtaaa taattataga tgaaatccaa
     2101 atccgactgg ctgaggttca gatctttcca atttacaatt gcatttacac ttttatcgtt
     2161 cacaatatat tcccttccct aattcgtgat tcactattca ttgattgtta gggaaaactg
     2221 ttgttgatcc aagccagtgg attgacatag ataccgttta cttttatttc ccaatgcagg
     2281 tgaggaccga ctgatctgcc ggtcgaacct attaatccaa ttaaatcacc tcgttttacc
     2341 tgttgcccgg cggtgacaaa agtctccgac tgatgagaat aggtagaata aactccccaa
     2401 ccatgatcaa taatggtgtg tttcccgtgt atcggcagat caccggaaaa caaaaccgta
     2461 ccatcagctg cggcataaat gttaatatta ttcgccgtac aaacaccaaa atccacccct
     2521 gaatggaaat tcgtataaga accgtcatta taagtacggc gatttccaaa gccagatact
     2581 aagcagggtt catctacggg ataagacatt ctgtctcccc aggattttat cggtgaggtt
     2641 tgagttattc cggcaagagt cgcctgatca acttcatttg aatgatcttc caacgtggaa
     2701 gcatcgactc ccgagacaac ttcataagta aagacacccg gttcaacaac aactttttgg
     2761 cgaatgtcaa aggattctcc gttcgaattc gaacctgaaa gcgatccggg taagataaag
     2821 ccaggtcagg aggagaaaaa gaagcgccag cggaaccccg taaagcatcc atcgggcgaa
     2881 accgatctca accccgtaca tctcctcaag aatggcggca aataccgcat tgggcggggt
     2941 tccgattatc gtggcaatcc ctccgatgga agcggcgtag gctatcccca gcatcagggc
     3001 ggccccgaac tggaatttcc cggtcctgac agcttccctg atatcagagc cgtgctccag
     3061 catctcgctg gtctggtaga tcaccgccaa tccgatcggg gtcatcatca tcgccgtagc
     3121 cgtgttcgaa atccacatcg aaagggcggc cgtcgaggcc atgaacccca ggatgatccg
     3181 ctcagggctc tcccccagag ccctgatcac cagcagggct atacggcgat gcaggttcca
     3241 cctttccatg gccagggcga aaaaaagccg cccatgaaaa gaaacac
//
LOCUS       DAOMIV010000208         3275 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31106_length_3275_cov_4.903727, whole genome shotgun sequence.
ACCESSION   DAOMIV010000208 DAOMIV010000000
VERSION     DAOMIV010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31106_length_3275_cov_4.903727"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..2035
                     /locus_tag="PKV59_05705"
     CDS             149..2035
                     /locus_tag="PKV59_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46283.1"
                     /translation="MAEQAEKKCQKTNKRKMSDWGSVLVILSIIEGCWSTATLFEIPS
                     EAGNAVFLGYSAERLAIAAICILGIIFLIGFFLLRKRGRYSVIIRQITFGGSALIFTL
                     CALIFLLNYSDIGQYDTLISSLVSRYGGIFVWGILFGIEIGATVIIDSKREDGGLNRL
                     LNPLYTAVLFGMAVVLFSLAFEYYDRVDWTRHMGHARAIFLAPAAVGILWALSVELIK
                     GKEAKSKANTVWLFLMIASLAFAVYRLTGFWIYRTDTPPKSYWDLLANAFLNRQLYLN
                     NPPTTHDLTMYNGKWYVPNPPLPAILLMPFVKWFGVEGINMTVVSAIIGALNTALIFL
                     ILHRAVQIGMIRCERSAILWLTVVFAFGTNHIWLAVTGQMWFISQLITITMIELSVLA
                     VISKWSGWLVGLFFGFAILARPNIFPIAFLLLGIYLWEQADFPKADWHKTINWGIKTA
                     FPVLICLCLLLSYNKLRFDDWFDFGYVTINGAPWILEAVQKYGMFHPHFFQTNLRVML
                     WQLPTLDFSGDRFFFQPGISGFSIFVMTPPLIYLFRRFRKNWWVIGAWASVILTILLL
                     LFYHNTGAEQVGYRYLLDAIVPIMLLMGLGVDRKVNWFFKVLAMIGIAVNLLSVYWWF
                     IART"
     gene            2188..>3275
                     /locus_tag="PKV59_05710"
     CDS             2188..>3275
                     /locus_tag="PKV59_05710"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQP46284.1"
                     /translation="MNDLIAQVILNVPAKDGIFDYSIPEELIGKIKTGCMVVVPFRQT
                     YMQGIVLSLNDESAVSNLRPVTQLVDEMPVLSFAQLELAKKLSTRCLAPLGDCVNVFL
                     TEKIRKISEDNYTLVKKPDQKSISDEKPTEKERRKTLIRERLISLFPEENSVLAQSEI
                     DQKMEGENWKNAMDSLIREGVIRKDPVFSLPDNKLPVRRVAHLSATAETIESEWNVLS
                     RQENALKRRQELLLCLKSTADGIPVTDLLRQTGATQVDIKALEKKHLISIAEEEAEGD
                     YTGLESDTYPGSEILLNAEQQKALEMIEQGLRATEQKVPILVHGVTGSGKTEIYIRAT
                     EQAIAQGKQVLVLVPEISLTPQILGRFSR"
BASE COUNT          843 a          635 c          824 g          973 t
ORIGIN      
        1 gctgatgttg ctattgggtg taggggtcgg taagaaaccg gggatcctgt tcaaggcgct
       61 gacgatattg ggtgtagcga tcaacctgct ctccacctac tggtggtata tcggcagagc
      121 ctaaaagata atctcgaagg acacggacat ggcagaacaa gctgaaaaga aatgccaaaa
      181 aacgaataag aggaagatgt cagattgggg ctccgttttg gtgatcctgt ccataataga
      241 agggtgttgg tcgacagcca cattatttga aataccctct gaagcgggga atgccgtttt
      301 tctcggctat tcggcggaac ggttggcaat tgcagcgatc tgtattctgg ggataatctt
      361 cctaatcggt ttctttctat tgagaaagcg gggacgatat tctgtaatta ttcggcagat
      421 aacatttggg ggaagcgcac tgattttcac tctgtgtgct ctgatatttc ttctgaatta
      481 tagcgatatt ggacaatatg atacgttaat ttcgtctctt gtatcccgat atggagggat
      541 atttgtatgg gggatactgt tcggcataga aatcggggcg acggtgatca tcgacagcaa
      601 aagggaggat gggggactga acaggctgtt gaacccgctg tatacggctg ttctgtttgg
      661 gatggcagtg gtcctgtttt ccctggcatt tgaatattat gaccgggtag actggactcg
      721 tcatatgggc catgcgcgag caatattttt agcaccggcg gcagtgggga tcctgtgggc
      781 gctatccgta gaattgatca agggaaaaga agcgaaatca aaagcaaata cagtctggtt
      841 gtttttgatg attgcctcgt tggctttcgc cgtatatcga ttgaccggtt tttggattta
      901 tcgtacggat actccgccga agagctactg ggatctgttg gcaaatgcgt ttttaaacag
      961 gcaactgtat ttaaataacc cgccgacaac gcatgatctg acgatgtata acgggaagtg
     1021 gtatgtgccg aatcccccct tgccggcaat tcttttgatg ccgttcgtga aatggtttgg
     1081 cgttgaagga attaacatga cggtggtatc cgcaatcatc ggtgccttga atacagcgtt
     1141 gatttttctt attttgcacc gagcggttca gatcgggatg atccgttgtg agcgatccgc
     1201 tattctttgg ttgacagtcg tttttgcctt cggaacaaac catatttggt tggccgtgac
     1261 cggacagatg tggtttatca gccaattgat tacgataaca atgattgaac tatccgtttt
     1321 ggcggtgatt tcaaaatgga gcgggtggct ggttggttta tttttcggtt ttgcaatact
     1381 tgccagaccc aatatattcc cgattgcatt cctattatta gggatttatc tttgggaaca
     1441 ggctgatttt ccgaaagcag attggcacaa aacaataaat tgggggatta aaacagcttt
     1501 ccccgtgctg atctgtctct gtctgttgct ctcttacaat aaattacgtt ttgatgactg
     1561 gtttgatttt ggatatgtca caataaacgg tgccccttgg attttggaag ccgttcaaaa
     1621 atatgggatg tttcatcctc atttctttca aacaaattta cgggtcatgc tgtggcagtt
     1681 gccgacactg gacttttccg gtgatcggtt tttcttccaa cccggcatat ccggtttcag
     1741 catttttgta atgactccgc ctttgatcta tctgtttcga cgttttcgaa aaaattggtg
     1801 ggttatcgga gcctgggctt ctgtcattct taccatttta ttgctattgt tttatcacaa
     1861 taccggagct gagcaggttg ggtaccggta ccttttggat gccattgtgc ccatcatgtt
     1921 gctgatgggc ttgggggttg atcgaaaggt aaattggttt ttcaaagtgt tggcgatgat
     1981 cgggattgcg gtgaatctcc tgtcggttta ttggtggttt attgcaagga cctgaccgga
     2041 ttatcctttc cctgaattcg tctatgccgg aaatatttat ttcggcactc aattcagccc
     2101 ttttcctttt tccgattcta ttattttatt ttcagggtta tcttcatccg aaaatttttt
     2161 gcggatcact cgggttaaaa ttatagaatg aacgatttga ttgctcaagt gattcttaat
     2221 gttccggcaa aagatggaat ttttgactac agcattccgg aagaactgat tgggaaaatc
     2281 aaaacaggat gcatggtagt cgttccgttt cgtcaaacat atatgcaggg aatcgtcctt
     2341 tctctgaatg atgagtctgc agtatcgaac ttgcggccgg ttactcagtt ggtggatgaa
     2401 atgcctgttt tatctttcgc acagcttgag ttggcaaaaa aactctccac gcgctgttta
     2461 gctcctttgg gcgattgtgt caatgtcttc ttaaccgaaa aaattcggaa aatatccgaa
     2521 gataattaca cccttgtgaa aaagccggat caaaaaagca tttccgatga aaaaccgact
     2581 gaaaaagaaa ggcggaaaac gcttattcgt gaacggttaa tcagcctttt cccggaagaa
     2641 aattcggtat tggctcaatc tgaaattgat caaaaaatgg aaggggaaaa ctggaaaaat
     2701 gcgatggatt cgttgattcg ggagggtgtg atccgaaaag atccggtttt ttctttaccc
     2761 gataacaaac ttcctgttcg tcgtgttgca catctttcgg caaccgctga aacgatcgaa
     2821 tccgaatgga atgtactttc ccggcaggaa aatgctctca aaagaaggca ggaattattg
     2881 ttatgcctga aatcaacggc agacggcata ccggtaaccg acttactgcg gcaaacggga
     2941 gctactcaag tggacataaa ggctcttgaa aagaaacatt tgatttcgat tgccgaagaa
     3001 gaagcggaag gagactatac cggattggaa tcggatactt atcccggctc tgaaattctg
     3061 ctgaatgccg agcaacaaaa agcacttgag atgattgagc agggattgag agcaacagaa
     3121 caaaaagtgc cgattctggt ccatggtgtg accggttcgg gaaaaacaga aatctatatc
     3181 cgcgcaaccg agcaggcgat cgcacaggga aaacaggttt tagtcttggt tccggagatt
     3241 tcactgaccc cgcagatttt aggacgcttt agtcg
//
LOCUS       DAOMIV010000209         3258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31320_length_3258_cov_3.213550, whole genome shotgun sequence.
ACCESSION   DAOMIV010000209 DAOMIV010000000
VERSION     DAOMIV010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31320_length_3258_cov_3.213550"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKV59_05715"
     CDS             complement(<1..696)
                     /locus_tag="PKV59_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQP46285.1"
                     /translation="MSDFEFVNAPTVDSAIEALAQWGKKACLVAGGTNVMVDIRAKKI
                     KDGTLINIRDIESLKKITYTDGKVSIGALTIIEEIAKSEIIKQHAPCLFAAANVFADP
                     TTNRSATIGGNVGNASASGDTIPPLMVLNAIVHLKSKRGDREVPINEFIIKNGVTTRE
                     PDEMITYFTFESQPNTGFIKLGARRSMTISIATVAAYIEMDKQSGIVKNCRIALGGVA
                     PKPVRERMQKKHLS"
     gene            complement(689..1174)
                     /locus_tag="PKV59_05720"
     CDS             complement(689..1174)
                     /locus_tag="PKV59_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQP46286.1"
                     /translation="MSLHEITLKVNGKTHKLVVKSNELLLNVLRDRLYLTGTKYGCGL
                     AECGACTILNGDRSIMSCLVPAVVADGWEITTSEGLADGDKLSPVQEEFINQGAIQCG
                     FCTPGMVVTSTALLRENPDPDEDFIRNYLRGNYCRCTGYAAMIKAVQVAAQRMKEEKN
                     E"
     gene            complement(1171..2061)
                     /locus_tag="PKV59_05725"
     CDS             complement(1171..2061)
                     /locus_tag="PKV59_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HQP46287.1"
                     /translation="MNTVYLEKEYDYVKPKTLAEALEILETKKNVKIFAGGTDLIVKL
                     KIGSPIEMDTMLDINGIEELKKVTSDKEGITIGATEKISVLEKNEDIINRFPALYEAF
                     ISMASISLRNMATLIGNLCNASPGADTAGPVICYGGSLLIKNHAGERRIKAEDFFAEG
                     GKTVLEPNEIVTSVNIPAPMENTGAAFIKLSRVKADLAKISVSVVLTRENNVVGSCRI
                     AMGSVAGKPLFLKEIGDGLVGKPLTKELIIETAEKISKAIKPRDGGNRTTAAYRIETS
                     KIISRDALEQAWERSGGKLS"
     gene            complement(2058..>3258)
                     /locus_tag="PKV59_05730"
     CDS             complement(2058..>3258)
                     /locus_tag="PKV59_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQP46288.1"
                     /translation="EEMNFSPVDLRLKNYRGIGDEFWGQGPTIRSIIKSCGVEESLVE
                     GRKVFKYDEKIEPSKKTGVKRRGIGLARGFHCSGTGGPKEGEVIDYSSAMIKINEDGS
                     VDLCTPVTDHGGGTLDALRKIVSETLKVPYDLVGLSPADTMNTGYDVCTHATRGVYCG
                     GGAVLNVARKVKEMLLEVAGRLMDEHACDLELVLDEEAHQGRVQVPGYPQKSMTIGQI
                     AKQAQNNSWGTISYIDSYRQKNCPPCFTTHFVEVEVDTETGIITVPRVLIYGDCGVAM
                     NPDLVKGQLTGSFNRGFAYTVYEKLITDEKTGALKIKGLISDYKLPSTTEMPYVGNMQ
                     VELCHTYEPTGPYGAKGIGEAALASVQAAVANAIYNAIGIRFYKLPITPEVVLAALKE
                     KEGASHS"
BASE COUNT          806 a          829 c          620 g         1003 t
ORIGIN      
        1 cgataaatgc tttttctgca ttcgttcccg aaccggtttc ggtgcaactc cccctaatgc
       61 gattctgcaa ttttttacga ttccggattg tttatccatt tcgatataag cagcaacggt
      121 tgcaatagaa attgtcattg accgccgtgc tccgagtttt ataaaaccgg tattcggttg
      181 tgattcgaat gtgaaataag tgatcatttc atccggttcg cgagtggtta ccccattttt
      241 tataataaac tcattaatcg gaacttcccg gtcccctctc ttgcttttca aatgaacgat
      301 cgcattcaat accatcaaag gaggaatggt atcgccgctt gccgatgcat tgccgacatt
      361 tccgccgatg gttgcggatc gattggtggt aggatcggcg aagacattgg ccgccgcgaa
      421 taaacaaggt gcatgttgtt tgataatttc ggacttggca atttcttcaa taatcgttaa
      481 cgctccaata ctgaccttcc cgtcggtata agttattttc ttcaatgatt caatatcacg
      541 aatatttatc agagtgccat cttttatttt ctttgcccga atatcaacca tgacatttgt
      601 tccgccggct acaagacagg ctttttttcc ccattgagct aacgcctcaa tggcagaatc
      661 aacagtcgga gcattaacaa actcaaaatc actcattttt ttcctctttc attcgttggg
      721 ctgcgacttg gacagctttt atcattgcgg catatccagt acatcggcaa tagtttcctc
      781 tgagataatt tcgaatgaaa tcctcatcgg gatccggatt ttcgcgcaat aatgccgttg
      841 aagttaccac cattcccggt gtacaaaaac cgcattgaat agctccctga ttaataaatt
      901 cttcctgcac cggagataat ttatcgccgt ctgcaagtcc ttccgatgtg gtaatttccc
      961 atccatcggc taccacggcg ggaacaagac aactcataat gctgcgatca ccatttaaaa
     1021 tcgtacaggc accgcattca gccagaccgc acccgtattt tgtcccggtc aaatatagcc
     1081 ggtcccgcaa tacatttaat agcaactcat tactttttac gactaattta tgagttttgc
     1141 cgtttacttt caaggttatt tcatgcagac tcatgataat tttcctccgc ttcgttccca
     1201 agcttgctcc aaagcatctc tggaaataat ttttgatgtt tcaatacggt atgcggcagt
     1261 tgttcgattt ccgccatctc gtggtttgat cgcttttgat attttttccg ccgtctcaat
     1321 aatgagctct ttcgttaatg gcttcccgac cagtccatca ccgatttctt tcaggaataa
     1381 cggtttgccg gcaacggatc ccatcgcaat ccggcaagat ccgacaacgt tgttttccct
     1441 tgtaaggact acggatacac tgatttttgc cagatcggct ttaactctgc tcaatttaat
     1501 gaaagccgca ccggtattct ccatcggagc gggaatattt actgaggtaa caatttcatt
     1561 cggctccaag acggtttttc cgccttccgc aaaaaaatct tccgctttta tgcgccgttc
     1621 tccggcgtga tttttgatca gcaggcttcc gccgtagcag ataacaggtc ccgcggtatc
     1681 ggcaccggga gaggcattac aaagatttcc aattaatgtc gccatatttc tgagggaaat
     1741 cgaagccatg gatataaacg cttcataaag cgccggaaag cgattgatga tatcttcgtt
     1801 tttctctaga acagaaattt tctcggtagc tccgattgtg attccttctt tatcggaggt
     1861 aacctttttt aattcttcaa tcccgttgat gtccagcatc gtgtccatct cgatcggcga
     1921 accgattttc agtttaacga ttaaatccgt tccaccggcg aatatcttga catttttttt
     1981 ggtttcaaga atttccaatg cctcagccaa ggttttcggt tttacatagt catattcttt
     2041 ttcgaggtaa acggtattca tgaatgactt gctccttcct tctctttcaa agcagccaaa
     2101 actacttccg gtgtaatcgg taatttgtag aaacggatcc cgattgcgtt ataaatggca
     2161 ttagcgacgg cagcttgaac agaagctaaa gctgcttcac cgatcccttt tgccccataa
     2221 ggtccggtcg gctcataagt atggcacaat tcgacctgca tgttcccaac atacggcatt
     2281 tcggttgttg acggtaattt gtaatcgctg attaatcctt tgattttcaa cgcgcctgtc
     2341 ttttcatctg tgatcagttt ttcataaaca gtataggcaa atcctcggtt aaaagaaccg
     2401 gtcaattggc ctttcaccaa atcaggattc attgcaactc cgcaatcgcc ataaattaga
     2461 acccgtggga cagtaataat acccgtctcg gtatccactt ccacttcgac aaagtgagtg
     2521 gtaaaacaag gcggacaatt tttctgtcga taactgtcaa tataggaaat tgttccccaa
     2581 ctgttattct gagcttgttt tgcgatttgt ccgattgtca tgctcttttg cggatatccc
     2641 ggtacttgaa cgcgtccctg atgtgcttcc tcatccaaca ccaattccaa atcacaggca
     2701 tgctcatcca tcagcctccc ggcgacttct aaaagcatct ctttcacttt tctggcaaca
     2761 ttcagcaccg cacccccgcc gcaatatact ccgcgggtcg catgcgtaca cacatcatat
     2821 ccggtgttca tcgtatcagc gggagaaagc ccgaccaaat cataaggaac tttcaatgtt
     2881 tcagaaacga tttttcgcag agcatccagt gtcccgccgc cgtgatccgt tacaggggtg
     2941 cacaaatcca cactgccatc ttcattgatt ttgatcatgg cactgctgta atcgatgact
     3001 tccccttcct ttggtccgcc tgttcctgaa caatggaacc cccgcgctaa tccgatacct
     3061 cttcgcttta cacccgtctt cttggaaggt tcaatttttt catcatattt gaaaactttt
     3121 cgaccttcta ccaaactctc ttccacgccg caacttttaa taattgatcg aatggtcggg
     3181 ccctggcccc aaaattcatc gccaattccg cgataattct tcagacgcag atcaaccgga
     3241 gaaaaattca tttcttcc
//
LOCUS       DAOMIV010000210         3255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31357_length_3255_cov_4.240000, whole genome shotgun sequence.
ACCESSION   DAOMIV010000210 DAOMIV010000000
VERSION     DAOMIV010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31357_length_3255_cov_4.240000"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /gene="metG"
                     /locus_tag="PKV59_05735"
     CDS             complement(<1..1687)
                     /gene="metG"
                     /locus_tag="PKV59_05735"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQP46289.1"
                     /translation="MKQPVLVTAAWPYANAFIHVGNLSGAYLPADIFARYQRLKGRDT
                     LFVSGTDAHGTPITVRADDEKTTPEVIYKRYHESFLDMQQKVGISYDLFTSTHTENHF
                     KVSQDIFLALQENGYLYKATEKQWYAPSQEKFLPDRYVEGTCYICGYDNARSDQCDKC
                     GNLLDPEKLINPRSKIDGSTPELRETEHFYLDLGKLQNRVVDFLKSREDYWRPNVIRQ
                     SLGQILAEDLHGRAITRDLEWGIPVPVQDAVGKCLYVWFEAVIGYLSASIEWAKLQGT
                     PDIWKDWWEDDTCKSYYFIGKDNIPFHAVIWPAQLIGTGTKFGVYFEGKENKPFVLPF
                     DVPANEFMNLEGKKISGSRNWAIYVNDFLERYDPDPLRYYLTVNMPESQDTDWDWEDF
                     LKRNNNELVATWGNLANRVISFANKHWGQVPDPGTLEMEDQDLIDTIESGFLTVGDEY
                     EAVHLRAALAETMRLATEVNRYLDIAAPWQLIKTDKEAAGRKIYTALQAINNLKVLFS
                     PVLPFTSQKLHNMLGFPSNLFGEQVIKTVRDNLGEHLVNTYDTAKAIGSWQPTD"
     gene            1864..3162
                     /locus_tag="PKV59_05740"
     CDS             1864..3162
                     /locus_tag="PKV59_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQP46290.1"
                     /translation="MHFPLKLKQNFQRIPLQIWVIFAGSLIFSIGSSMAWPFLNIYLR
                     ERLNLPLRTTTLLISLRAVTGILASFVAGTICDKFGRRGVMLASLLGGTVYYFLMVYA
                     QTIWQFAVLMGIWGAMDLFYPIGSNSMIADMVKAEDRLEVYSLMRMVNNAGIAIGPIF
                     GGILAIKSYSLIFYSASIGYFISFVIFLFTVKETLQKRTITGNEVKIPTKGYHDVLKD
                     GVFVSTILMAVFIYMGSSIVFNLLSLYASETYGILESQISYVFTVNALMCVFLQLGAM
                     KFTVRIHPMLVMVISSLFYTVGLLLYVVCANVIWYCICMGILTIGELLMSPTMLALTA
                     KRAPADARGRYMSIYNLSHPIGNAFGPALAGLLYDQFIPQSIWYSAGFYCLIAAIGFS
                     VLIGRIRMHPGYKRSPSMNNEAISTEFEENSFGEAFSLKR"
BASE COUNT          818 a          729 c          736 g          972 t
ORIGIN      
        1 ggtcggttgg ttgccaagag ccaatcgcct tagcggtatc ataggtgttc accaagtgct
       61 cgccgagatt atcccgaacg gttttaatta cctgttcacc gaacaaattc gacggaaaac
      121 cgagcatatt atgtaatttc tgagaagtaa acggcaaaac gggtgaaaaa agtactttga
      181 gattattaat agcctgcaga gccgtatata ttttccggcc ggctgcttct ttgtccgttt
      241 tgatcagttg ccaaggagcg gcgatatcga gatagcgatt gacttccgtt gccaagcgca
      301 tggtctccgc caaggcagcg cgcaagtgaa cggcttcata ttcatcaccg acggttaaaa
      361 agccgctttc gatcgtatca atgagatctt gatcttccat ttccaatgtt cccggatcgg
      421 gaacctgccc ccaatgcttg ttggcaaaag aaataacccg atttgccagg ttaccccaag
      481 tcgcaaccaa ttcattgtta tttcgtttca aaaagtcttc ccaatcccaa tcagtatcct
      541 gagattcggg catgttaact gtcaaataat atcgcaacgg atccggatca taccgttcca
      601 gaaaatcgtt gacgtaaatc gcccaatttc gactgccgga gatctttttc ccttccaagt
      661 tcataaattc gtttgcgggg acatcaaagg gaagaacaaa cggtttgttt tcctttcctt
      721 cgaaatatac gccgaattta gttccggtac cgattaactg tgccggccag ataacagcat
      781 gaaaggggat attatccttg ccgatgaaat agtatgattt acaggtatca tcttcccacc
      841 aatctttcca gatgtccggt gtaccctgca atttcgccca ttcgatcgaa gcggataaat
      901 aaccgatgac cgcttcaaac cagacgtata aacactttcc cacggcatct tgaactggaa
      961 ccggaattcc ccattccagg tcacgggtga ttgcccggcc gtgtaaatct tcggcgagaa
     1021 tctgccccag cgactgtcga ataacattgg gacgccaata atcctcgcgg gatttcaaaa
     1081 aatcaaccac ccgattttgg agttttccga gatcgagata aaagtgctcc gtctctcgta
     1141 actccggtgt ggaaccgtct attttcgaac gcggattaat taatttttcc ggatccagca
     1201 gattcccgca tttatcacat tggtcacttc gcgcgttatc atacccgcag atatagcagg
     1261 ttccttcgac atatcggtcc ggcagaaact tttcctgact gggggcatac cattgctttt
     1321 cggtggcttt atacaaatag ccgttctctt gcaaggcgag aaagatatcc tgagagactt
     1381 taaaatggtt ttccgtatgt gtggaagtaa aaagatcata tgaaatgccg actttttgct
     1441 gcatatccag aaatgattcg tgatatcgct tatagatgac ttcgggggtt gttttttcgt
     1501 catccgctcg aacggtaatc ggtgtcccgt gtgcatccgt gccggataca aaaagggtgt
     1561 ctctgccttt caatcgctga tatcgcgcaa agatatcggc gggaagataa gcacctgaaa
     1621 gattgccgac atgaatgaaa gcgtttgcat agggccaggc tgctgtgaca agtacgggtt
     1681 gtttcatttt attacctctt gaattcgact tctcagcgtt tcttccttcc cgactgagat
     1741 tctaagctca aattctatca taaacagcct tcaatttctt ccgttgtacg aatgttcagg
     1801 atgaaaccca cgatgctatg ataaacttgt caagctgatc aattttacgg tcgctgaggt
     1861 ccgatgcatt ttcctttaaa actgaaacaa aattttcaac ggattccgct ccaaatctgg
     1921 gtgatttttg ccggatcgtt aattttttcc atcgggtctt ccatggcttg gcctttcctg
     1981 aacatctacc tccgcgaacg gttaaatctg ccacttcgga caacgaccct attgatatct
     2041 ttaagagcgg tgacggggat tctggcatct tttgtcgcgg gcacaatctg tgataagttc
     2101 ggacggcggg gtgtgatgct ggcttctctt ttgggaggaa ccgtctatta ttttttgatg
     2161 gtatatgccc aaacgatctg gcaatttgcg gtcctgatgg gcatttgggg ggcgatggat
     2221 ttgttttacc ccatcgggtc caattccatg atcgcagaca tggtgaaagc cgaagaccgt
     2281 ttggaagttt attctctgat gcgaatggtc aataatgccg gcatcgccat cggcccgatt
     2341 ttcggcggaa tattggccat taaatcttac tctctgattt tttattcggc atcgatcggg
     2401 tattttatct ctttcgttat tttcttgttc acggtaaagg aaacgcttca aaaacgcacg
     2461 ataacgggaa acgaagtaaa aattccgaca aaagggtatc acgatgttct aaaagacggc
     2521 gtatttgtct cgaccatctt aatggcggtc tttatttata tgggttcatc catcgttttt
     2581 aatttgcttt cgctctatgc ttcggaaact tatggaattt tggaaagcca aatcagctat
     2641 gtctttacag tgaatgcttt gatgtgcgta ttcttgcaat tgggagcgat gaaatttacc
     2701 gtcagaattc atcccatgct ggttatggtg atctcatcgc tgttttatac ggtcgggctt
     2761 ctcctgtatg tcgtttgtgc gaatgtaatt tggtactgta tctgcatggg aattttgacg
     2821 atcggcgaat tattgatgtc ccccaccatg ctggctttaa ctgccaaaag agcaccggcg
     2881 gatgcccgcg ggagatatat gagtatttac aatctttctc atccgatcgg gaatgcattt
     2941 ggaccggcgt tagccggctt attatatgat caatttatcc cgcaatcgat ttggtacagt
     3001 gccggttttt attgtctgat cgccgcaatc gggttttcgg tgcttatcgg aagaataagg
     3061 atgcatcctg gttacaaaag atctccatcg atgaataacg aggcgatttc aacggaattc
     3121 gaagagaatt cgtttggcga agctttttct ctgaaacgtt gatggcagta tcgaatttta
     3181 cggtgataga cgggtttcga gaaaattcac aggtattgtg gttcccgaga aataacccca
     3241 agcttttctg attaa
//
LOCUS       DAOMIV010000211         3254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31370_length_3254_cov_2.680213, whole genome shotgun sequence.
ACCESSION   DAOMIV010000211 DAOMIV010000000
VERSION     DAOMIV010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31370_length_3254_cov_2.680213"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKV59_05745"
     CDS             <1..216
                     /locus_tag="PKV59_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46291.1"
                     /translation="RHETFSADGYPAYWAGAHFSIDGKNYETGHLFVQVNNEVFQIMS
                     YATRGIVEKYRQELNHITESFSLSRSE"
     gene            497..1213
                     /locus_tag="PKV59_05750"
     CDS             497..1213
                     /locus_tag="PKV59_05750"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HQP46292.1"
                     /translation="MTKKSKKTKPDFSLPESGRKRTFTLYTENFFELIKLSLIFILFS
                     LPVFTVFASATAMNYVISKIMNDRLVFIWDDFIKAFKSNFLRSTLYGILSSIIFIILI
                     MGWNISSQAATLSSTLRMILKVFYIFLGVILILHTVYFYPIQAWIDLPFRSVLRNAML
                     IQFVAYKTTLKIFLILIVVIPFIWIFRPITYFLIPFFSATIVFLLNSYAMQPIQKYLI
                     IKKPEEDPAFNDSNDIHINI"
     gene            complement(1203..>3254)
                     /locus_tag="PKV59_05755"
     CDS             complement(1203..>3254)
                     /locus_tag="PKV59_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQP46293.1"
                     /translation="WGQRYTLQKFGILSDCFWLPDTFGYSAAIPQIMRGFGIKYFLTT
                     KMAWNDTNRFPYNTFVWEGIDHSQVLAHLFVMDTWADPQGLLERVNGIQYEDGLINKQ
                     DCSERLVAFGYGDGGDGPTAEMIETARRTEDLEDCPKTYYSSLSGFMKHIEETYPQLP
                     RYKGELYLELHRGTLINKQQIKKNNRKSEVALHNLELLETVQAVQAGRAASVEKTNQL
                     WEILLKNQFHDILPGSCIAVAHDQSIAETNSLLETAAEMTDQILLSDNRSACFDAVFN
                     PLGFEHKTPFEIPMEEMYTDPKGELKTQQIVNLDNETKLVVSGLSQKPFCRTAFQLEP
                     GISSAETPFLLNGNRLETAFAWIQFDEDGYICSMIDKSNHRELCRGIPFGTFASTEDV
                     PAKWDGWDIDADYELKLKAEKGNLISREVVSNGAVELRIRSRYKVCRNSEIDVDQIFY
                     AESPLIEYDVRLDWRDPHYLLKVLFDTTISVPFATFDIQFGNIKRTTTRNNTVEQARF
                     EVCCHKYVDLSEPAFGFTVVNDCKYGVGVRDGLIGLSLAKGGTRPDERGDVGVYRFRY
                     GFFPHTGGYCAKEVILPAYCFNYPSLPMRGTNISMESLLTTDAANVIIETIKPCEDSE
                     KAFIARIYESEGSFTSTKLHAGFQIQSLQVCNMMEQPICDCDPNLVFQPFEIKTIKVK
                     Y"
BASE COUNT          922 a          751 c          627 g          954 t
ORIGIN      
        1 cggcacgaaa ctttttcggc agatggatac cctgcttatt gggccggtgc tcatttttcg
       61 atcgacggga aaaattacga aaccggtcat ctgttcgttc aggttaataa tgaagtcttt
      121 cagattatgt cctatgcgac gagaggaata gttgaaaaat atcgtcagga actgaatcac
      181 ataacagaaa gtttttcact gtcacgatcg gaatagttga acttgcaaat tccctcataa
      241 attcatcgta gcgatgagcc gtcggtctct aaagagaaaa atcgatggct catcgccgtt
      301 tttgtttcat tataagaatc aggcctcttt ttcgaaagcc aaagaataaa tccctgcccg
      361 aatccaaatc gttgggttta ttcgtaggat cgtaatttat acgaacgata tcggtaataa
      421 ttttctcgag gtttcatcta taattcaata agaaagcagt cataaccggt taaaagaaag
      481 aatcggaggc atatcaatga cgaagaagtc aaaaaagact aaaccggatt tttctcttcc
      541 cgaatcaggt cggaaaagaa catttaccct ttataccgag aactttttcg aactcattaa
      601 gctcagtttg atttttattc tcttttcgtt gcctgttttc actgtttttg catcggcgac
      661 agcaatgaat tatgtcatct caaaaataat gaatgatcga ttggttttta tttgggatga
      721 ttttattaag gcattcaaat caaatttttt acgaagcacg ctttacggca ttctttcttc
      781 gattatattt attatattga tcatggggtg gaatatcagc agtcaagctg ccacgttgtc
      841 gtcaacacta cgaatgattc tgaaagtatt ctatattttc ctcggagtaa ttctgatcct
      901 gcatacagtg tatttttatc cgatccaggc ttggattgat ttgccctttc ggtctgtatt
      961 aagaaacgcg atgctcatcc aatttgttgc ctataaaaca accttaaaaa ttttcttaat
     1021 tttaatagtt gtaattccgt ttatttggat ttttcgtccc atcacctatt ttctgatccc
     1081 atttttctct gccacaatcg tcttcctact gaattcatat gccatgcaac ccattcaaaa
     1141 gtacctgata ataaaaaagc cggaggaaga tccggctttc aatgactcga atgacattca
     1201 tatcaatatt taacttttat cgttttgatc tcaaagggtt gaaaaaccag atttggatcg
     1261 caatcacata tcggttgttc catcatgtta caaacctgca gcgattgaat ttggaacccc
     1321 gcatgaagtt tcgttgaagt gaaagaacct tcggattcat aaatccttgc gataaaagct
     1381 ttttcggaat cttcgcaggg tttaatcgtc tcgatgataa cattcgccgc atccgtcgtt
     1441 aacaaggatt ccatactgat attcgtccct ctcatcggta gcgaagggta attaaagcaa
     1501 taagcgggaa ggataacttc ttttgcgcag tagccgccgg tatgagggaa aaagccataa
     1561 cgaaaacgat aaactccaac atctccgcgt tcatccggcc gcgtaccgcc tttagccaac
     1621 gaaagaccga tcaatccgtc tcgaacgcca acgccatatt tgcaatcatt caccaccgtg
     1681 aaaccaaaag ccggttcact gagatcgacg tatttatgac agcacacctc aaaccgcgcc
     1741 tgctcaacgg tattgtttcg agtggttgtc cttttgatgt tgccgaattg aatatcaaat
     1801 gtcgcaaacg gaactgaaat ggtcgtatca aacaacacct ttagcaaata atgcggatcg
     1861 cgccaatcta atctaacatc atattcgatc aaaggagatt cggcataaaa aatctgatca
     1921 acgtcaattt ccgaattacg acagacctta tatcgggaac gaattctcaa ttccaccgca
     1981 ccgtttgaaa caacctcccg cgaaatcaga ttcccttttt ccgctttcaa tttcagctca
     2041 taatcggcat cgatatccca gccatcccat tttgccggaa catcttcagt ggaagcaaaa
     2101 gttccgaacg ggatgccccg gcataattcc cggtgattgg atttgtcaat catcgaacaa
     2161 atatatccgt cttcatcgaa ttgaatccaa gcaaaagccg tttccaaccg gtttccgttc
     2221 agaagaaacg gtgtctcagc ggatgaaata ccgggttcga gctgaaaagc tgtccggcaa
     2281 aaaggctttt gcgataatcc cgaaactacc aatttcgttt cattatccaa attcacgatt
     2341 tgctgggttt tcaattcacc cttcggatcc gtatacattt cttccatagg aatctcaaag
     2401 ggggttttgt gctcaaaccc caacggattg aaaaccgcgt caaaacacgc gcttctattg
     2461 tcagataata aaatctgatc ggtcatttca gcggctgtct ccaagagcga attcgtttcg
     2521 gcaatggatt gatcatgggc tactgcgata caacttcccg gtaaaatatc atgaaattgg
     2581 tttttcagta aaatttccca caattgattt gtcttctcaa cggaggcagc tctgccggct
     2641 tgaacagcct gaacggtttc aagcagctca agattatgaa gcgccacttc acttttccga
     2701 ttattctttt taatctgctg cttgttgatt agcgtcccgc gatgcagctc cagatagagc
     2761 tctcctttgt agcggggaag ttgcggataa gtctcctcga tgtgcttcat aaaccctgag
     2821 agagaactgt aataggtttt ggggcaatct tccaaatctt ccgttcgccg tgccgtttcg
     2881 atcatttccg cggtcggacc gtccccgcca tccccatatc cgaaggcaac cagccgctcg
     2941 ctgcaatcct gtttattgat caacccgtct tcgtattgaa tgccgttgac tcgctccaat
     3001 aatccctgcg gatccgccca agtatccata acaaataaat gagcaagcac ctggctatga
     3061 tcgatacctt cccaaacaaa ggtgttatac ggaaatcggt tagtgtcatt ccatgccatt
     3121 ttcgtggtca ggaaatactt gatcccgaag ccacgcatga tttgaggaat tgccgccgaa
     3181 tatccaaaag tatccggcag ccagaagcaa tcggataata tgccgaattt ctgcaaagta
     3241 tatcgttgtc ccca
//
LOCUS       DAOMIV010000212         3252 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31389_length_3252_cov_4.944636, whole genome shotgun sequence.
ACCESSION   DAOMIV010000212 DAOMIV010000000
VERSION     DAOMIV010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31389_length_3252_cov_4.944636"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="PKV59_05760"
     CDS             <1..802
                     /locus_tag="PKV59_05760"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQP46294.1"
                     /translation="DHFGIQTLITIGGDDTLSFSVRLHKEGFPVIAIPKTMDNDVFGT
                     DYCIGFSTAITRSVEVITNMRTSLGSHERIGVIELFGRNSGETSLLAAYLSGADRAVI
                     PEVHFNIDKLTDMLVEDRRRNPSNYSLTTISEGAIMEGGDVIETGEMDAYGHRKLGGV
                     GMVVAEAIKKRSNINIMYQQVGYMMRSGAPDSLDRMVATSYGNLAAQLLIRNDTGRMV
                     ALHGGKYTTVPVNMVLAGKKCVDVPSFYDIEEYRPHIKDFLGVPMFLS"
     gene            819..1769
                     /locus_tag="PKV59_05765"
     CDS             819..1769
                     /locus_tag="PKV59_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HQP46295.1"
                     /translation="MIVTTKKLFEHAYGKYAIGAYNINNAEQIIGLFRGNLDSKAPFI
                     VQISKGAREYTSKIMLEGLIRSADQMFPEAIFAVHLDHGDEETCYDCINSGFYSSVMI
                     DASALSFEDNIAVTKRVVDAAHAKGIVVEAELGKLGGVEEHVSVNDADAKLTDPDSVK
                     EFVDRTHVDSLAVAIGTSHGAFKFSGTQSLHFDVLAEIQKRVPGFPLVLHGSSSVPQE
                     EIDRINAAGGNIKGAKGVDPSQFKRAAQLGVTKINIDTDGRLVWMRVHREYFRDHPEN
                     FDFRPIGKIFMAEYAKFIADKNEKLGSAGQLDSVREFLSK"
     gene            1912..2691
                     /gene="surE"
                     /locus_tag="PKV59_05770"
     CDS             1912..2691
                     /gene="surE"
                     /locus_tag="PKV59_05770"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQP46296.1"
                     /translation="MQKPLEIVVTNDDGIFAPGILALASTAMKIGNVSVIAPDKNWSA
                     SGHQKALGRPLRVDPVSFPLDIPAYATDGGPSDCVALAGLGFLDKKIDLVLSGINPTS
                     NVSRDITYSGTVTSALESTIWNMKGIAFSLDAGGIKIKEIDFSETEPMILTVVRTFLQ
                     HELPPFTILNVNLPYKVKKPNPDYVITREGSRYYRDELIRQVDPFGKPYYWFGGDPPY
                     GDEEEGTDCGELARGNISITPLQLDLTAEQLIPSLQQWEWK"
     gene            2701..3207
                     /locus_tag="PKV59_05775"
     CDS             2701..3207
                     /locus_tag="PKV59_05775"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HQP46297.1"
                     /translation="MIQKRCILLFSSLLLFVLSGCASNPTAIPAPVYTAPLPTRIVAT
                     KVIIQNEPDCIDNFYYIDDVNYPDGTYVEPGQKFLKEWEVMNNGSCNWIDGYLLRFVS
                     GEQMGAPDSVPIPHVEVGKKGKFSVEFTAPKEPGTYRSIWKPFNNRNRSFGETVYLEI
                     VVNGYPAE"
BASE COUNT          867 a          731 c          820 g          834 t
ORIGIN      
        1 ggatcatttt ggtatccaga cgctcattac gatcggtggg gatgataccc tgagcttctc
       61 ggttcggctc cacaaagaag ggttcccggt cattgcgatt cccaagacga tggataatga
      121 tgttttcggg acggattact gcatcggatt ctcaaccgcc atcacccgtt ccgtcgaagt
      181 cattaccaat atgcgaacct ctctcggctc tcatgaacga atcggcgtga tcgaactttt
      241 cggtcggaat tcgggtgaga ccagcttgct ggccgcatat ctgtcgggag ccgaccgcgc
      301 tgtcattccg gaagtgcatt ttaatattga caaattaacg gacatgctcg tcgaagaccg
      361 gcggcgcaat ccgtcaaact attcgctgac aaccatttcc gaaggagcga tcatggaagg
      421 cggcgatgtg atcgagacgg gtgagatgga tgcttacggg caccgcaagt tgggcggcgt
      481 cggaatggtc gtcgcagaag cgattaagaa gcgatcgaat atcaatatta tgtaccaaca
      541 ggtaggatat atgatgcgtt ccggcgcacc ggattcgttg gatcgaatgg tcgcaaccag
      601 ttacgggaac cttgccgcgc aattgttgat ccgaaatgat accggcagaa tggttgcttt
      661 gcacggagga aaatatacga ccgtcccggt caatatggtt ctggctggga aaaaatgcgt
      721 tgacgtcccg tctttttatg atatcgaaga ataccggccg cacattaagg atttccttgg
      781 tgtgcctatg tttttaagct gattctgatg gaggaataat gatagtaaca acgaaaaaat
      841 tatttgaaca tgcttacggc aaatatgcca tcggagcata taacatcaat aacgccgaac
      901 aaattatcgg cttgttcagg ggtaaccttg acagtaaagc tccttttatt gtgcaaattt
      961 cgaaaggcgc acgggaatat accagcaaga ttatgctgga aggcttaatt cgcagcgccg
     1021 atcaaatgtt cccggaagca atttttgcgg ttcatttgga tcacggcgat gaagaaacct
     1081 gctatgactg cattaatagc ggcttttaca gttccgtaat gattgatgcc tccgcattaa
     1141 gctttgaaga taatattgcg gtcaccaaac gagtagtcga tgccgcacat gccaaaggga
     1201 tcgtagtgga agcagagctc ggcaaattgg gcggtgtcga agagcacgtt tccgttaatg
     1261 atgcggacgc caaactgacc gatccggatt cggtaaaaga gttcgtcgac agaacccatg
     1321 tggattcgct ggcagttgcg atcggaacca gccacggtgc attcaaattc tccggaaccc
     1381 aatctctgca ttttgacgtt ttggcggaga ttcaaaagcg agtccccggt ttcccgttgg
     1441 ttttgcacgg ttccagttcg gttccgcagg aagaaatcga tcgaatcaat gcggccggcg
     1501 gcaatattaa aggcgctaag ggcgttgatc cgagccaatt caaacgcgct gcccagttgg
     1561 gcgtgacaaa gatcaatatc gataccgacg gacgtctggt ttggatgcgt gtccatcgtg
     1621 aatatttccg cgatcatccg gagaatttcg atttccggcc gatcggtaaa attttcatgg
     1681 ctgaatatgc aaaattcatc gccgataaaa atgagaaact cggatctgcc ggacagttgg
     1741 acagcgttcg ggaatttctc tcaaaataaa cagactctga ctcaaaaaac ggcgtgcttc
     1801 ggggaaaccc ggcacgctgt tttttttcaa acaccgattc attttcaagc caaaaagctt
     1861 cgagctgttg gtagaatagt aatagattct tttagatcaa ttgaggattt aatgcaaaaa
     1921 cctttggaaa ttgttgtaac gaatgatgac ggaatttttg cgccggggat tctggccttg
     1981 gcttcaaccg ctatgaaaat aggaaatgtt tccgtcatag cccccgataa gaactggtct
     2041 gcttccgggc atcaaaaagc actggggcgc ccgctgaggg tagacccggt ttcttttcct
     2101 ctggacattc cggcttatgc caccgacggg ggcccgtcgg attgtgtcgc actggcgggg
     2161 ttgggctttt tggataagaa gatcgatttg gtgctctccg gaattaaccc cacttcgaac
     2221 gtgagccggg atattacata ttccggaact gtcaccagcg cattggaatc aacaatctgg
     2281 aatatgaaag gaatcgcttt ttcgctggat gccggcggca tcaaaataaa ggagatcgat
     2341 ttttccgaga cggaaccgat gatcttaacg gttgttcgaa cttttttaca acatgaatta
     2401 ccgcctttta cgattttgaa tgtcaacctc ccttataaag taaaaaagcc gaatccggac
     2461 tatgtcatca cgcgggaagg ctcccgatat tatcgggatg aattgatccg ccaagtcgat
     2521 cctttcggaa aaccgtatta ttggttcgga ggagatcccc catatgggga cgaagaggaa
     2581 ggaacggatt gcggcgagct tgcccgcggg aatatcagta tcactcccct gcaactggat
     2641 ttgaccgccg aacagctaat tccgtctctt cagcaatggg aatggaaata aatcggcggc
     2701 atgatacaga aacgctgcat tcttcttttt tcttctttat tattgtttgt actttccgga
     2761 tgcgcaagca atccaacggc aatccccgca cccgtataca cagctccttt gccaactcgg
     2821 atcgttgcca ccaaagttat tatccaaaat gaaccggatt gcatcgataa tttttactac
     2881 attgatgatg tcaattatcc ggacggcact tatgtggaac ccgggcaaaa atttctgaaa
     2941 gaatgggaag tgatgaataa cgggagctgt aactggattg acggctatct gttacggttt
     3001 gtttcgggtg aacagatggg tgcgcccgat tccgttccga ttccgcatgt ggaagtcggg
     3061 aaaaaaggga aattttcggt agaatttacc gctccgaaag aacccgggac atatcgatca
     3121 atttggaaac cgtttaacaa tcggaatcgc tccttcggag aaacggttta tttggaaatc
     3181 gttgtcaacg gatatccggc agaataaatt cgaaaatcga tattacataa aaaaaccgca
     3241 ccccgtgccg tt
//
LOCUS       DAOMIV010000213         3251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31396_length_3251_cov_4.757822, whole genome shotgun sequence.
ACCESSION   DAOMIV010000213 DAOMIV010000000
VERSION     DAOMIV010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31396_length_3251_cov_4.757822"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKV59_05780"
     CDS             complement(<1..434)
                     /locus_tag="PKV59_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQP46298.1"
                     /translation="MKGIILAGGKGTRLYPLTRVVSKQLLPVFDKPMIYYPLSMLMFA
                     GLKEVLVISTPEDLPLYRRLLSDGSQWGMKFEYAEQPKPEGLAQAFLIGEGFLNGDSA
                     CLVLGDNIFYGSSIPTKLRTAAALEKGAVIFAYEVNDPERYGV"
     gene            complement(498..1571)
                     /gene="rfbB"
                     /locus_tag="PKV59_05785"
     CDS             complement(498..1571)
                     /gene="rfbB"
                     /locus_tag="PKV59_05785"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HQP46299.1"
                     /translation="MKNVLVTGGAGFIGSNFVRYLLQREPDVKVVTLDLLTYAGKMEN
                     LEDLPRPESHVFVQGDICNRELVDQLLAEHAIDTIVHFAAESHVDRSILGPMPFIQTN
                     IIGTFTLLEAAKNAWKDKPGEFRFHHISTDEVFGALQSGDPAFEEGTPYNPHSPYSAS
                     KASSDHLVRAYYSTYNLPITISNCSNNYGPYQFPEKLIPLMILNGITGKPLPVYGKGE
                     QVRDWLHVMDHCEAIYLILKKGKIGETYCIGGDNQPTNLEIVQLICEILDDKVPRADG
                     KSHTSQIQYVSDRPGHDFRYDINTNKIQRELGWKPAHELRTGLEETIEWYLNHSAWVD
                     SVLDKDEFSSWINTNYQERDSSF"
     gene            complement(1568..2113)
                     /gene="rfbC"
                     /locus_tag="PKV59_05790"
     CDS             complement(1568..2113)
                     /gene="rfbC"
                     /locus_tag="PKV59_05790"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQP46300.1"
                     /translation="MDFIPLSIPDVILIQPDVHRDERGFFFESWQKNKFAKAGIDQEF
                     VQDNHSRSSKDVLRGLHYQIRQPQGKLIRAIVGEVFDVAVDLRRSSPSFGKWVSANLS
                     SENFNMLWIPPGFAHGFLVLSDVAEFVYKATDFYAPQYERCILWNDRDLGIDWPISGH
                     EPILSEKDSKGKLFREAEVYL"
     gene            complement(2141..2875)
                     /locus_tag="PKV59_05795"
     CDS             complement(2141..2875)
                     /locus_tag="PKV59_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46301.1"
                     /translation="MNQNIAIQTEDLGICFKMPSEPISGTKELFIKIAKKRIKINTFW
                     ALRHVNLSLNEGESIGIMGKNGAGKSTLLKAIARVLTPKEGRVYVRGKVFPMLELGAG
                     FNMELTGHENIYLYGNILGLTNEKVTQLYDSIVEFSELREFIHSPLRSYSTGMVARLA
                     FATATAVQPDILLADEILSVGDKDFQDKCMDRMRNYLNNGTTIVFVSHSPNSVKQICK
                     KGLWLEHGQVRMTGSAEEVADAYAAS"
     gene            complement(2884..>3251)
                     /locus_tag="PKV59_05800"
     CDS             complement(2884..>3251)
                     /locus_tag="PKV59_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46302.1"
                     /translation="AFSICFAVYSILLSLFVGISLVVATITPFLNDFSQIWSVIITLW
                     MYLTPLFYPVNIIPEHLIGIYQLNPMYIYVTLFRDPLLYAKIPELSLWLGGLIYGVII
                     FAFGWWLFTKNSNEFAYRA"
BASE COUNT          968 a          707 c          621 g          955 t
ORIGIN      
        1 accccgtatc gttccggatc attcacttca taagcaaaaa tgaccgctcc tttttcaagt
       61 gcggcagctg tacgcaattt ggtcggaatg gaagaaccat aaaagatgtt atctcccaag
      121 acgagacatg ccgaatctcc gttaagaaaa ccttcaccga taaggaaggc ttgtgcaaga
      181 ccttccggtt tcggctgttc ggcatattca aatttcatcc cccattggct accatccgaa
      241 agaagccgtc tgtaaagggg aagatcttcc ggtgtactga ttacaagtac ctctttcaac
      301 cctgcaaaca ttaacatcga cagcggataa tagatcatcg gtttatcaaa taccggtaat
      361 aactgtttgc tgacaacccg ggtcagcggg tagagacgtg tgccttttcc gccggcaaga
      421 atgattcctt tcatttgcta tatttccttt tattttgtca atcgattatt agaagggaag
      481 aaaaattcct tctcttttta aaatgatgaa tctctttctt gatagttggt atttatccag
      541 gatgaaaatt catctttgtc caaaacagaa tcaacccaag cggaatgatt caaataccat
      601 tcgattgttt cttccaatcc ggtacgtaac tcatgagccg gtttccatcc caattcccgt
      661 tgaatcttat ttgtattgat atcatagcgg aaatcatgac ccggacggtc ggaaacatat
      721 tgaatctggc tggtatgcga ttttccgtca gccctcggga ccttatcgtc aagaatttca
      781 cagattaatt gaacaatttc aaggtttgtc ggctgattat ctccgccgat gcaataggtt
      841 tcaccgattt ttcctttttt caaaatcagg taaattgctt cgcaatgatc catcacatgc
      901 aaccaatcgc gaacttgctc accctttcca tataccggta acggttttcc ggttatgcca
      961 tttaatatca tcaaaggtat aagcttttcg gggaactgat aaggtccgta attgttgctg
     1021 caattactga tcgttatcgg aagattatat gtcgagtaat atgcccgaac caaatgatcg
     1081 ctggatgctt tggatgctga ataaggcgaa tgagggttat agggtgtccc ttcttcaaaa
     1141 gccggatcgc ccgattgaag agcgccaaaa acttcatctg tggaaatatg atgaaaacga
     1201 aactcgccgg gtttatcttt ccaggcattt tttgctgctt ccagtaatgt gaaggtaccg
     1261 atgatatttg tctggataaa cggcatcggg cctaaaatcg atcgatctac atgtgattct
     1321 gcggcaaaat gcacgatcgt atcaatcgca tgctcggcga gaagctggtc aaccaattct
     1381 ctgttacaaa tatctccctg aacaaagaca tggctttcag gacgcggcaa gtcctccaaa
     1441 ttttccattt ttccagcgta ggttaagaga tctaaggtca cgactttgac gtccggttca
     1501 cgttgtaata aatatcggac aaagtttgag ccgataaaac cggcgccccc ggtgactaag
     1561 acatttttca taaatagacc tcagcttctc gaaatagttt accctttgaa tctttttcag
     1621 ataaaatcgg ttcatgtccc gaaatcggcc aatctattcc caaatctcga tcattccata
     1681 agatacatct ttcgtattgg ggggcataaa aatcagtggc tttataaacg aattcggcaa
     1741 cgtcacttaa aaccaaaaaa ccatgagcaa agccgggagg tatccatagc atattgaaat
     1801 tttctgagct gaggttggcg ctcacccatt ttccgaaact cggtgaacta cgacgcaaat
     1861 caacggcaac atcgaaaact tcacctacta ttgctcgaat caattttcct tgtggttgtc
     1921 gaatttgata atgcaaccct ctgagtacat cttttgaaga acgcgaatgg ttatcttgaa
     1981 cgaactcctg atcaattccg gcttttgcga atttattttt ttgccaactt tcaaaaaaga
     2041 acccacgttc atcgcgatgc acatccggtt ggatcaaaat gacatcggga atagataaag
     2101 gaataaaatc catttttaat atcctatttt atttgtaatt ttaactggca gcataagcat
     2161 cggcaacttc ctccgcactc ccggtcattc ttacttgtcc atgttcaagc cataatcctt
     2221 tcttacatat ttgtttaaca gaattcggac tgtgagagac aaatacaatt gttgttccgt
     2281 tatttaaata gttgcgcatg cggtccatac atttatcttg aaaatcttta tcaccaaccg
     2341 ataaaatctc gtctgccaat aaaatatcgg gttgaacagc ggttgcggtg gcgaatgcca
     2401 gccttgcaac cattccggtt gaataggaac gcaacggaga atgaataaat tctcttaatt
     2461 cggaaaattc aacaatactg tcgtaaagtt gggtgacttt ttcattggtc aaccccagaa
     2521 tatttccgta aaggtaaata ttttcatgac cggttaattc catattaaaa ccggcaccga
     2581 gttcgagcat tgggaagact ttcccgcgga catatactcg cccttctttg ggagttagga
     2641 cgcgggctat ggctttcagt aaagtacttt tcccggcacc gttttttccc ataattccga
     2701 tcgattctcc ctcattaagc gaaagattga catgccggag cgcccaaaat gtattgatct
     2761 taatcctttt ctttgcgatt ttgataaata actctttcgt acccgaaatg ggttcggaag
     2821 gcattttaaa gcatatgccc aaatcttcgg tttgaattgc gatattttga ttcatcaaaa
     2881 ttcttatgcc ctatatgcaa attcattgga attttttgta aaaagccacc atccgaaagc
     2941 aaaaataatt acgccataaa tcaatccacc taaccataga gaaagttccg gtatttttgc
     3001 ataaagcaac ggatcccgaa ataaagtgac gtaaatatac atcggattaa gttgatagat
     3061 tccgatcagg tgctccggaa tgatatttac cggataaaac aatggcgtta aatacatcca
     3121 taatgtgata ataaccgacc aaatttgcga aaagtcattt aaaaatgggg tgattgttgc
     3181 aactacgaga cttatcccaa caaaaagaga taataaaatg gaatacacag caaaacaaat
     3241 cgaaaacgca a
//
LOCUS       DAOMIV010000214         3249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31434_length_3249_cov_3.451472, whole genome shotgun sequence.
ACCESSION   DAOMIV010000214 DAOMIV010000000
VERSION     DAOMIV010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31434_length_3249_cov_3.451472"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..134
                     /locus_tag="PKV59_05805"
     CDS             12..134
                     /locus_tag="PKV59_05805"
                     /inference="COORDINATES: protein motif:HMM:NF018161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46303.1"
                     /translation="MTRKRKLLEKQKRGKKRMKMVGNVEIPQEAFMAILQLDEE"
     gene            140..1168
                     /locus_tag="PKV59_05810"
     CDS             140..1168
                     /locus_tag="PKV59_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HQP46304.1"
                     /translation="MSDEPDKKKSGIWKQMLPGILISVVIILILVFVVDFGDLIASFR
                     NVSVRLIMIVFALQSVAFIFRALAWRVTLEGIPTWAQCFWTVTEGYLLNLLPLRLGEI
                     GRAVIMGGVIHRSPFFVFSTVLLERLFDVIITLIMLLATIPLVSGADFSPNLYYVLIS
                     IFLIGLVLLFLIAKNQENFVKRLEKIIKPESKFGRFLYPKLQSLLNGLSVLAQPKKFI
                     IWLFWILMTWVCWIATMLVCMHAFFPNLPVWSSLFVQSITALGGAIPSAPAGLGVIEG
                     AFVAALRIFGINQSEALGFGMFNHSMGIITPVIWGLIGFAVQGQSFSQVFSGLRKVNL
                     SEEDAKND"
     gene            1161..2189
                     /locus_tag="PKV59_05815"
     CDS             1161..2189
                     /locus_tag="PKV59_05815"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQP46305.1"
                     /translation="MTKKYFITGGAGFIGSNFAADLLRKNREVTIFDNLSRSGSRHNI
                     AWLRRQFGADSFRLIQGDVRDADALEKAVGNADAVAHFAAQVAVTTSVTNPREDFEIN
                     AAGTFNLLEAVRKSGRQPVVLYTSTNKVYGGMENVRVIEREEDYVYEDFPMGIAESQP
                     LDFHSPYGCSKGTGDQYVRDYSRIYDIPTVVFRQSAIYGPRQFGMEDQGWVSWFIIAV
                     LLKKPITIYGNGKQVRDLLFIDDLVRAYHAAIDAPETTAGQIFNIGGGPNNVISVWKE
                     FGPILEELCGEKIPVNYSNWRPGDQVIYVSDIRKAERLLNWRPEVSVKEGITRLYHWA
                     EENRNLFA"
     gene            2186..>3249
                     /locus_tag="PKV59_05820"
     CDS             2186..>3249
                     /locus_tag="PKV59_05820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP46306.1"
                     /translation="MKILVALTYYTPYVSGLTIYADRIARAWAKRGHEVTVLTSRHDS
                     KLPEEEIVEGVRVIRAPIFMRISKGAIMPAFWRQAGKLAKESNVVNIHLPQFDAGWVA
                     RQAKRQKTFSVLTYHCDLQMPAGLINQISNQAVLRMNDIAGKNCDAIVAYTEDYANYS
                     FFLPKYMEKVRIIPPPVELPQVNEAEVEEFRMKRNPEDKSPLIGMACRFAADKGVEIL
                     LDALPKILNTYPNACVFFAGPYDNVLGERAYYERLKPRLDAMIAEGHWKFLGSLPPKE
                     MAKFYQIIDLLAMPSLNRTDAFGLVQIEAMMNGKPVVASNLPGIRVPVTRHGMGKIAE
                     IGNAEDLAQKIVEVLQEKKD"
BASE COUNT          870 a          627 c          823 g          929 t
ORIGIN      
        1 acgggggaga tattacccgt aaaagaaaat tgttggaaaa acagaagcgc gggaaaaaaa
       61 ggatgaagat ggtggggaac gttgaaatcc cacaggaagc atttatggcg atattacagt
      121 tggatgagga ataaagtcaa tgagcgacga acccgataag aaaaaatccg gtatttggaa
      181 acagatgctg ccgggaatcc tgatcagtgt tgtaataatc cttattttgg tctttgtcgt
      241 tgatttcggt gatttgattg cttctttcag aaacgtttcc gtccgattaa tcatgatcgt
      301 attcgccttg caatccgtgg cttttatttt ccgagcctta gcttggcggg tcaccttgga
      361 aggcattcca acctgggctc aatgtttttg gacggttaca gaaggctatt tgctcaattt
      421 attgcctttg agattagggg aaatcggtcg ggctgtcatc atgggagggg ttattcatcg
      481 cagtcccttt tttgtttttt caaccgttct tttagaaaga ttgtttgatg tcatcatcac
      541 tttgattatg cttcttgcga ccataccgtt ggtttccggt gccgattttt caccgaattt
      601 atattatgtt ttgatttcga tatttttgat cggtttggtt cttttgtttt taattgcaaa
      661 gaaccaggaa aactttgtca aacggttgga aaaaatcatc aaaccggaat caaaattcgg
      721 ccgattttta tatccgaaat tgcagtcgct tttaaacgga ttgagtgtgt tggcacagcc
      781 gaagaaattt atcatctggc ttttttggat tctgatgact tgggtttgct ggattgcgac
      841 catgttggta tgtatgcacg cctttttccc gaatcttccc gtatggtcgt ctttatttgt
      901 tcaaagtatt acggcattgg gaggagctat cccctccgca cccgcgggat tgggtgtcat
      961 cgaaggcgcc ttcgtcgctg ctttaagaat tttcggtatc aatcaatccg aagcactcgg
     1021 tttcggtatg tttaatcatt caatgggaat cataaccccg gttatctggg gcttaatcgg
     1081 ctttgcggta caggggcaaa gtttttcaca ggtattttca ggtttacgaa aagtgaacct
     1141 ttctgaagag gatgcaaaaa atgactaaaa agtattttat aaccggagga gccggtttta
     1201 tcggttccaa tttcgcggca gacttattaa gaaagaaccg ggaagtaacc atttttgata
     1261 atttatctcg aagcggatca cggcataata tcgcctggtt acgcagacaa ttcggtgcgg
     1321 attccttccg tttgattcag ggagatgtcc gtgatgcaga tgcgttggaa aaagcggttg
     1381 ggaacgccga tgcagtggct cattttgccg cacaggttgc cgttaccact tcggtgacca
     1441 atccacgcga ggattttgaa ataaatgcgg cagggacgtt taatttgctg gaagcagtgc
     1501 gaaaatcagg caggcagcca gtcgttttat atacctccac caataaagtc tacggaggga
     1561 tggaaaacgt aagagtcatc gaacgtgagg aagattatgt ttatgaagat tttccgatgg
     1621 gcattgccga aagtcagccg ttggattttc attctcctta cggttgttcg aaagggaccg
     1681 gcgatcaata tgtgcgagac tattcacgca tctatgatat tccgaccgtc gtttttcgcc
     1741 aaagcgcgat ttacggtccg agacagttcg gtatggaaga tcagggatgg gtttcttggt
     1801 ttatcatcgc ggttctactg aaaaaaccga taacaattta tggaaacggg aaacaggttc
     1861 gcgatctgct gtttatcgat gacttggtca gagcatacca tgcggcgatc gacgcaccgg
     1921 aaacaaccgc cggacagata tttaatatcg gcggagggcc gaacaatgtg atttccgtat
     1981 ggaaagaatt cggaccgatt ttggaagagc tttgcggaga gaaaatcccg gtcaattaca
     2041 gcaattggcg acccggggat caagtgattt atgtatcgga tatccggaaa gcggaacgct
     2101 tgctaaattg gcgacccgaa gtctccgtta aggaaggaat aactcgtttg tatcattggg
     2161 cagaggaaaa ccggaacctt ttcgcatgaa aattctggtt gcgttaactt attacacccc
     2221 ttatgtcagc gggttaacca tatatgccga tcgaatcgcg agagcatggg cgaaacgcgg
     2281 gcatgaagtg accgttttaa cctccaggca tgattcgaaa cttccggagg aagaaatagt
     2341 agagggcgtg agagtaattc gcgctcccat attcatgcgt atcagcaaag gtgccattat
     2401 gcctgctttt tggcggcaag ccggaaaact ggcgaaagaa tcgaatgtag tcaatattca
     2461 tcttccgcaa ttcgatgccg gctgggtagc tcggcaggca aagcgtcaga aaactttttc
     2521 cgttttaacc tatcattgcg atctgcaaat gccggccgga ttgatcaatc agatttcgaa
     2581 tcaagcggta ttacggatga atgatatcgc cggtaagaat tgtgatgcca tcgtcgcata
     2641 taccgaagat tatgcaaatt attctttttt tctgcctaag tatatggaaa aagtcagaat
     2701 tattccgccg ccggttgaat tgcctcaagt gaacgaagcg gaagtcgaag aatttcggat
     2761 gaaacggaat ccggaagata aatcaccgct gatcggtatg gcatgccggt ttgccgcgga
     2821 taaaggcgtt gaaattctgt tggatgcatt acccaaaata ttgaatacat atccgaatgc
     2881 ctgcgtattt tttgcgggtc cttatgataa tgttttgggg gaacgcgctt attatgagcg
     2941 tttgaaaccg cgattggatg cgatgattgc cgaagggcat tggaaatttt tgggaagttt
     3001 gccgccgaaa gaaatggcaa agttttatca aatcatcgat ttattggcaa tgcccagtct
     3061 gaatcgtacg gatgctttcg ggttggtaca gattgaagcg atgatgaacg gaaaaccggt
     3121 tgttgcctcc aatcttcccg gtattcgtgt tccggtcact cgacacggaa tgggaaaaat
     3181 tgccgaaatc ggcaatgcgg aagatctggc tcaaaaaatt gtcgaagttt tacaagagaa
     3241 aaaagatta
//
LOCUS       DAOMIV010000215         3234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31641_length_3234_cov_4.550802, whole genome shotgun sequence.
ACCESSION   DAOMIV010000215 DAOMIV010000000
VERSION     DAOMIV010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31641_length_3234_cov_4.550802"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..906)
                     /locus_tag="PKV59_05825"
     CDS             complement(187..906)
                     /locus_tag="PKV59_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46307.1"
                     /translation="MKEKLIVLIIIAAVLAAFSPASQNFRGPNRKSTPKSAPTKNVLP
                     PPLIKSTAIPDFTETPETLEPIQIPEATEIPKVPEILESTETPETMEILPAQLGQKIN
                     CGNKFQFTFYYQPLMSKSNSGQVAKGKFLVARVLITNLTDRTLEGLPARSFRLHGIID
                     GVDQEYKLHIANSFITSDRWDVGLITDTIQAGGMLDTFLVFDVPGKADNWYLKFEPIA
                     KSSEGPFCRVAIQVPKINYVD"
     gene            complement(952..1689)
                     /locus_tag="PKV59_05830"
     CDS             complement(952..1689)
                     /locus_tag="PKV59_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46308.1"
                     /translation="MKRHFWILSVILLLFMIFGASAQSEGDDQSRQTGDNLLKNSEKV
                     FPRLLINEDFSENLPLDVYEDESNGIKITSVIQQKNGVRKATNYVDGLSKDIEGLSAF
                     YSETYYGTLSEFHLHVEVSILNEFPDSTGGCFIEYTNEAVVGENSRQTVEIILGSRID
                     TYQTKSGSRVLTTLYDLSGTHEVGKIYNIDIIRYGGIGYFFIDGVFLIAYQDNIKENL
                     SWMTGPIVGIGGEQTTCIFDKIWVRRK"
     gene            2094..>3234
                     /locus_tag="PKV59_05835"
     CDS             2094..>3234
                     /locus_tag="PKV59_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46309.1"
                     /translation="MDNENKSNIENEKLNEEPKDSFVVTHHSILCGGKKISYTATAGR
                     MILKEEIVQKEGDQKNQYEGEKPRAEIFFVAYTLDGVDDPTKRPITFAFNGGPGSASI
                     WIHMGLLGPKRVLLNDDGTPLPPPARLTENEYSVLDMTDLVFIDPIGTGFSRAVKGDK
                     PNDYWGWTKDVESVGDFIRLYISRNQRWGSPKFIAGESYGTTRTTGLSDYLSDKYGMY
                     LNGIVLVSTALDFATLDFNDGNDLPYILYLPSYAATAWYHKKLKAKYQEMPLEKYLEE
                     VRHFAGGEYLQALFNGSRMAEKQIEQVADKTANYIGLTREYVLRANLRVTLPRFSKEL
                     LRSERRTVGRYDGRFIGIDRDAAGEEPEDDPAGYEINGTFAGAFND"
BASE COUNT          965 a          632 c          665 g          972 t
ORIGIN      
        1 atcgatttca tgatgaaggt tgcctcttta ttgattcgat gtattcattt aaaacagtcg
       61 ttttttcttg atttaatgtt gcacttatca tgccaacttt cgaattgcta tctttaaaag
      121 aaaagaccgg agtaccgggc tatttcggcg ttccgatctt aataaaatta ttcttccaat
      181 atatgattaa tctacataat taattttcgg aacctgaatc gcgacgcgac aaaaaggacc
      241 ttcggagctt tttgcgatcg gttcaaattt caaataccaa ttgtctgctt tgccggggac
      301 atcaaatacc aagaaagtat ctaacatacc gccggcttga attgtatcgg taatcaggcc
      361 gacatcccag cggtcactcg taatgaagct gtttgcgata tgtaatttat actcttgatc
      421 gactccatcg ataattccat gtaatcgaaa cgatctggca ggaaggcctt cgagcgttcg
      481 gtctgtcaaa ttggttatga ggacacgcgc taccaaaaat tttccttttg ccacctgacc
      541 gctattcgat tttgacatca gcggttgata atagaaagta aattgaaatt tattcccgca
      601 attaattttt tgccccaact gcgcgggtaa gatttccatt gtctccggag tttcggtact
      661 ttcaagaatt tcgggaactt tcggaatctc agttgcttcg gggatttgta tcggctcaag
      721 agtctccggt gtttcggtaa aatcggggat ggcggttgat tttatgagag gcgggggaag
      781 gacatttttg gtcggagcac ttttcggagt actctttcga ttaggtcccc tgaaattttg
      841 tgaagcggga gaaaaagctg ccaagactgc agcaataata atcagtacga ttaatttttc
      901 tttcatagct atttatccct tacgaaacta accccgacaa tatttaaatg attattttct
      961 gcgaacccaa attttgtcga agatgcaggt tgtctgttct ccgccgatcc ctacgatcgg
     1021 tcccgtcatc caagataaat tttctttaat attatcttga taggctatca aaaatactcc
     1081 atcgatgaaa aaataaccga taccgccata tcgaatgatg tcaatattgt agatttttcc
     1141 gacttcatgg gtaccactca aatcataaag cgttgttaaa actcgacttc ctgattttgt
     1201 ctggtaggta tcgattcttg atccgagaat tatctctaca gtttggcgag aattttctcc
     1261 gacgacagct tcgtttgtat actcaataaa acaaccgccg gttgaatccg gaaattcatt
     1321 caaaatactt acttctacat gaagatggaa ttccgataac gtcccgtaat aagtttcgct
     1381 gtaaaaagca cttaatcctt caatatcttt actgagtcca tcaacataat tagtggcttt
     1441 tctgactcca tttttctgct gaataacact ggtgattttt attccatttg attcatcttc
     1501 ataaacgtcc agcggtaaat tttcggagaa atcttcgtta atcagtaaac ggggaaatac
     1561 tttctctgag ttctttaata aattatctcc ggtttgtctg gattgatcat ccccttcgga
     1621 ttgtgccgaa gcaccgaata tcataaacag taaaagaatt acggacagaa tccaaaaatg
     1681 tcttttcata attcattcct attccggata caaattttgt atctctaaaa actcagccta
     1741 tttaattcat taaatacaag attatgcaga tttttctgtt tagaaaaagt atatcatatt
     1801 caaaaaaaca gcaggcttga atttgcgaaa attgatcata aataggcacg gcgtaaagag
     1861 aaatatcttc ctataattat ctatcaacga aacaggaaga gccggaaatt tgaatggatt
     1921 tcgagtgcaa aataattcgg atgatcttat tttcaggctc cgaattttca aaaatttttg
     1981 ggatcaaaag aatagatcaa tactcttttt cttttcgact ttttttgtta tatagttaat
     2041 ggcattgaaa tgagaaatcg ggagataata gcaaaaaaac tgaggaacaa aaaatggata
     2101 atgaaaataa atcgaacatc gaaaatgaaa aattgaatga agagcccaaa gattctttcg
     2161 tcgtcactca tcattcgatt ctgtgcgggg gcaaaaagat ttcatatact gccaccgccg
     2221 gaagaatgat tttgaaagaa gaaatagtcc aaaaagaagg cgatcaaaaa aatcaatatg
     2281 aaggagaaaa gccccgagcg gagatatttt tcgtcgcata tacgttagac ggagttgatg
     2341 atccgacaaa acgcccgatt acctttgctt ttaatggcgg tccgggatcc gcatcgatat
     2401 ggatccatat gggtttgttg ggaccgaaac gagtcctcct gaacgatgac ggcactccgt
     2461 tacctccgcc ggctcggctg acagaaaacg aatattctgt tttggatatg accgacttag
     2521 tttttatcga tccgatcggt accggttttt cgcgtgccgt aaagggtgac aaacccaacg
     2581 attactgggg atggacgaaa gatgtcgaat cagtcggcga ttttatccga ctttatatct
     2641 ctcgaaatca acgatggggt tcgccgaaat ttattgccgg tgaatcgtac gggacgactc
     2701 gtacaaccgg tttatccgat tatctttcag acaaatacgg gatgtatctg aatgggattg
     2761 tactggtttc gaccgcactg gattttgcca cgctcgattt caacgatgga aatgatttac
     2821 cttacatcct ttacctgcct tcttatgctg cgactgcttg gtatcataag aagctgaaag
     2881 caaaatatca agaaatgcct ttggaaaaat atctggaaga agtgcgccat tttgccggag
     2941 gcgaatatct tcaggctttg tttaacggca gccgcatggc ggaaaaacaa atcgaacaag
     3001 ttgccgataa aacggcgaat tatatcgggc tgacacgcga atatgtattg cgggcaaatt
     3061 tacgggtgac attgccgcga ttttcgaaag aattattaag gagcgaacgc cgaactgtcg
     3121 gccgttatga tggaagattt atagggatcg atcgtgacgc tgccggcgaa gaaccggaag
     3181 atgatccggc cggttatgag ataaacggaa cttttgcggg agcattcaat gatt
//
LOCUS       DAOMIV010000216         3226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31760_length_3226_cov_5.129297, whole genome shotgun sequence.
ACCESSION   DAOMIV010000216 DAOMIV010000000
VERSION     DAOMIV010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31760_length_3226_cov_5.129297"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..757)
                     /locus_tag="PKV59_05840"
     CDS             complement(14..757)
                     /locus_tag="PKV59_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQP46310.1"
                     /translation="MKPVYEPTIGMLPVDTSYCPGCGHGIAHKLIMECITEKGRLGDT
                     IGVSPIGCSVVAHEFMNIEMCESAHGRASAVATGIKRVHPNHLVFSYQGDGDMASIGV
                     TEIVHAAIRGEKITTFFINNAIFGMTGGQMAPTTLIGQKATTCIDGRKKEVNGMPIRM
                     SEMLATIEGAVYIERVALNTPGNVRKAKKAVMKALEVQEKGLGFSLVEFISSCPTNWG
                     LSPIDALKWVGDNMIPYYPLGVFRSPEGM"
     gene            complement(754..>1152)
                     /locus_tag="PKV59_05845"
     CDS             complement(754..>1152)
                     /locus_tag="PKV59_05845"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQP46311.1"
                     /translation="NHLFEKYRKAEKDLVRYETVNTKDAEIVFVAFGTMARIVSEVIE
                     VLNAEGIKAGMIRPISLWPFPKAPFDELSPDKTKVVISTELNMGQMIFDVQLSLNGRI
                     PVRLINRVGGIVPTTDEIVERAKKILGEIK"
     assembly_gap    1153..1252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1253..1659)
                     /locus_tag="PKV59_05850"
     CDS             complement(<1253..1659)
                     /locus_tag="PKV59_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HQP46312.1"
                     /translation="MTEKVLMKGNEAFCEAAIRGGCRFYVGYPITPQSEIPEFMSHEM
                     PKRGGTFIQAESELGSINIAYGIGCAGGNVFVSSASPGIALMQEGLSFLCSAEVPVVL
                     LNVSRSGPGIGGIQPAQADYNQVTRGGGNGDYK"
     gene            complement(1656..1892)
                     /locus_tag="PKV59_05855"
     CDS             complement(1656..1892)
                     /locus_tag="PKV59_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQP46313.1"
                     /translation="MGKYLVKFNRDKCKGCELCVSFCPKKILEIDKVDMNAKGYHPAN
                     VKPGQMDECIGCLSCALMCPDNVISVYELSGDEK"
     gene            complement(1910..2617)
                     /locus_tag="PKV59_05860"
     CDS             complement(1910..2617)
                     /locus_tag="PKV59_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46314.1"
                     /translation="MIHIGDKRILLVAGHYGSGKTEFCINLAIDLAEQGIKTALVDLD
                     IVNPYFRTREKTQFLESKGIKVYGNFYKTEITMEIPALSAEVRTPLEDPTVYTIIDLG
                     GNSAGALVAIQFEKYFVEGTYEFLDVINKNRPETSTIEGAVNHFHDIEERTGLKFTGL
                     INNTHLLRETTIEDIQKGLEFCEELSNILKVPLLFTGCPNYLYEQLLSSKLMEKINHQ
                     ILPIHFIMRDTWLDKKL"
BASE COUNT          858 a          717 c          585 g          966 t
ORIGIN      
        1 tcctttgcca taattacatt ccctccggtg atctaaaaac tcccaacgga taatatggga
       61 tcatattatc tccaacccat ttaagggcat caatcgggct aagtccccag ttcgtcggac
      121 aggatgaaat aaattctact aaggagaatc ctaatccttt ttcctgaact tcgagcgctt
      181 tcatgacagc cttttttgct tttctaacgt taccgggagt atttaaagcc actctttcaa
      241 tataaactgc tccttcgatg gttgcgagca tttcggacat tcgaatcggc attccgttga
      301 cctctttttt ccgtccgtcg atacaggttg tggctttttg gccgattaag gttgtgggag
      361 ccatttgtcc gccggtcatg ccaaagatcg cgttattgat gaagaaagtt gtgatttttt
      421 ctccgcggat cgccgcatgg acaatttcgg tgactccgat ggaagccatg tccccgtcac
      481 cctgataact gaatacaagg tgattcggat ggactcgttt gatgccggtt gccaccgccg
      541 aagcacggcc gtgtgccgat tcacacattt cgatattcat gaattcatgt gcgacaacac
      601 tgcaaccgat cggtgagacg ccgatcgtat cgccgaggcg tcctttttcg gttatacatt
      661 ccataattaa tttgtgggca ataccatgcc cgcagccggg acaataactg gtatctaccg
      721 gcaacattcc aatagtcggt tcgtatactg gtttcatttg atttctccca gaatcttttt
      781 tgctctttcc acaatttcat ctgtcgtagg cacaattccg cccactcgat tgattaacct
      841 gaccggaatt cttccgttca aactgagttg aacatcaaaa atcatctgcc ccatattcaa
      901 ttccgtcgag attactacct tcgttttgtc gggagataat tcatcaaaag gagctttcgg
      961 aaacggccag agactaatcg gccgaatcat gccggcttta atcccctcgg cattgagaac
     1021 ttcgataacc tcggaaacga tccttgccat cgttccaaag gcgacaaaga cgatttcggc
     1081 atcttttgta ttgacggtct catatcgaac caagtctttt tccgcttttc gatacttctc
     1141 aaacaaatga ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnattttata
     1261 atctccgttt cctccgccgc gggtaacctg gttatagtca gcctgtgcgg gttgaattcc
     1321 gccgattccg ggaccgctgc gggagacatt taaaagaacc accggcactt cagccgaaca
     1381 aagaaaactg agcccttcct gcattaatgc aatgcccgga gatgccgatg aaacaaatac
     1441 atttccgcct gcacaaccga tcccgtaagc gatattaata gagcctaatt cgctttcggc
     1501 ttgaataaac gttcctccgc gtttcggcat ttcatgagac ataaattcag gtatttcact
     1561 ttgtggggta atcgggtagc cgacataaaa ccggcagccg cctcgaatcg cagcttcaca
     1621 aaaagcttcg tttcccttca tcaatacttt ttctgtcatt tttcatctcc tgacaattca
     1681 tagacgctga ttacattgtc cgggcacatc aaagcgcagc tcaggcaacc gatgcattca
     1741 tccatctgcc ccggttttac gttagcggga tgataaccct ttgcattcat atccaccttg
     1801 tcaatttcga gaatcttttt ggggcagaaa gagacgcata attcgcatcc tttacatttg
     1861 tccctgttga acttcacaag atacttgccc atatcatttc actctccttc taaagttttt
     1921 tatccaacca tgtatctcgc ataataaaat gaatcggtag gatttggtgg ttgatttttt
     1981 ccatcagttt agacgataac agctgctcat ataaataatt cgggcaaccc gtaaataaaa
     2041 gaggaacttt cagtatattt gaaagttcct cgcaaaattc cagacctttt tgtatgtctt
     2101 caatggtggt ttcacgaagc aggtgtgtat tattaatcaa tccggtaaat tttaagcccg
     2161 ttcgttcttc gatatcatga aaatgattta cagctccttc gatggtggat gtttcgggac
     2221 ggttcttatt gatcacatcc aaaaattcat aagttccttc aacaaaatat ttttcgaact
     2281 gaatggcaac taatgccccg gctgaatttc ctcccaaatc gataatggta taaacagtcg
     2341 gatcttccaa cggcgttcgt acttcggcgc tcagtgcggg tatttccatg gtaatttcgg
     2401 ttttatagaa attaccataa actttaattc ctttgctttc aaggaactgc gttttttctc
     2461 tcgttcgaaa atatggattt acaatatcca aatcaactaa agctgttttg atcccttgtt
     2521 ccgccagatc gatcgctaaa ttgatacaaa attctgtttt cccgctccca taatgtcccg
     2581 caaccagaag aattcgtttg tctccgatat ggatcatcta ttgatcatct ccccaaagtt
     2641 ttcttttttc tcccaaaaat gatttaccgc tgcaaaagat ctgatttcga aagcaaaatg
     2701 aatagggtga tacggtgcct gtaaataaga atcagtatcc cccggaataa aaacgttatt
     2761 caatgtattt ttatcaagga atcttctgcg attcttgatt tttgtaatta aactcgaaat
     2821 aaaaataccg gaagagagct cttttcccgt gaatgatgaa aaccaaccat aacaattatg
     2881 aaaaagaaag acaagattcg ttttgataaa aagaaatccg gattttagaa ataatatgga
     2941 tttttcagcg gattggactg tattttttct ttgctcaaaa ttcagcctat tcccactttt
     3001 gacggtggct acagttgaat cagctgtagc tgcctgttgc ttatatcgct caatgttttc
     3061 cactgacccc ccatccatct tctataattg attataaata taattacgaa taaggggtaa
     3121 tgcttttcga ggaatcttaa tttattgagg ataataatcc taaaagatta gtgatagaaa
     3181 tcagaaatga gaatttttgc acaataaaga ccggaaaata aaatta
//
LOCUS       DAOMIV010000217         3216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31911_length_3216_cov_3.914900, whole genome shotgun sequence.
ACCESSION   DAOMIV010000217 DAOMIV010000000
VERSION     DAOMIV010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31911_length_3216_cov_3.914900"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PKV59_05865"
     CDS             complement(<1..889)
                     /locus_tag="PKV59_05865"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF002823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HQP46315.1"
                     /translation="MNIDELIIKYPEILSLQSYRPFLWTNPHQNYAENLRFTITDIED
                     AENRFQRFAPFITEAFPETVLNKGIIESPLVEIRAMKAAMAERIGCSFSDHDRLFLKC
                     DHALPISGSIKARGGIYEVLKYAETLAIQAGLLRVQDNYRCLTEEKFKRFFSDYSLAV
                     GSTGNLGLSVGIMGRKLGFNVEVHMSTDAKKWKKELLRSVGATVYEHSGDFTQAVAQG
                     REQAKSNEKMHFIDDEYSTDLFLGYAVAAFRLKKQIAEQNISVDAEHPLAVIIPCGVG
                     GAPSGITFGLKQIFGKNVHC"
     gene            1101..1793
                     /locus_tag="PKV59_05870"
     CDS             1101..1793
                     /locus_tag="PKV59_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HQP46316.1"
                     /translation="MKVKALKAAFPFTIPVMIGYLFLGIGFGILLTGKGYPWWLSPLM
                     ALFIYAGSMQFVAIDIFSKAFNPLNALILTLTVNARHLFYGLSMLTKFKATDQKKPYL
                     IFALTDETYSLLCSTEPPQGIDRNWFYFFIALLDQIYWVTGCTLGSVAGSLVKFNTTG
                     IDFVMTALFVVIMVEQWESSKNHLPVLIGIGVSAICLLLFGKDLFILPSMISILAIVT
                     IFRKSIERRNVI"
     gene            1790..2122
                     /locus_tag="PKV59_05875"
     CDS             1790..2122
                     /locus_tag="PKV59_05875"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HQP46317.1"
                     /translation="MTIRQILITVSVIVFGTILTRSLPFFIFPDSKESPDYIKYPGQV
                     LPYAIIGMLVVYCLKNVNLLNFPFGIPEGIALIYVVLIHHRRHNLLLSIGGGTILYMV
                     MVQFLFPV"
     gene            2231..>3216
                     /gene="dnaN"
                     /locus_tag="PKV59_05880"
     CDS             2231..>3216
                     /gene="dnaN"
                     /locus_tag="PKV59_05880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQP46318.1"
                     /translation="MKVSVSQTNLAQALSIVSHAVATKSTLAILVNILITTEDGQLRL
                     SATNLEIGINYRIPADIIEEGAVTVPAKLMIDLINTLPNDTVYLELDTKTQTLSIRCL
                     QVITEIKGIDANEFPPMPVFEEDNAMTFDGSELRKMIQQVAFAASTDDARPILQGIYL
                     TAAGKTMTMVATDGFRIAIRNSTLEDSFAQPLSAIIPARALVELVRITNDSDKQIMMT
                     ISAGRNQVIFHLPQLDLASQTISGNYVDYKAIVPKSFQTTTVVSTAAFQKTCSQAMII
                     ARDSKYLTRFNIMGQENGMGKIQVTAQSEESGFYENYIDANVDGPDIQIAFNV"
BASE COUNT          863 a          709 c          677 g          967 t
ORIGIN      
        1 aacagtggac attcttcccg aagatctgct ttaaaccgaa agtgatcccg ctcggtgcac
       61 ctccgacgcc gcagggaata atcacagcca gcggatgttc ggcatctacg gatatatttt
      121 gttcggctat ttgttttttc agtctgaatg ccgccacggc atatccgaga aataaatccg
      181 ttgaatactc atcatcgata aaatgcattt tctcgtttga ttttgcctgc tcacgacctt
      241 gggctaccgc ctgagtgaaa tcccccgaat gctcgtatac cgtcgctccg acagatcgga
      301 ggagttcctt cttccatttc ttggcatcgg tcgacatatg aacctcgaca ttaaaaccga
      361 gttttcgtcc catgatcccg acactcagcc cgagattgcc ggtagagccg actgccaggc
      421 tgtaatcgga gaagaatcgc ttaaatttct cttcggtcaa gcagcgataa ttatcttgta
      481 cccgaagtaa gccggcttga atggcaagcg tttcggcata tttcaggact tcataaatcc
      541 cgcctctcgc tttgatcgag ccggaaatcg gaagggcatg atcgcatttc agaaaaagtc
      601 tgtcatggtc tgaaaaagaa caaccgattc tttcagccat tgcggctttc atggcacgaa
      661 tttcaaccaa gggagattca ataatacctt tattcaaaac agtttcaggg aatgcttccg
      721 tgataaacgg agcgaatcgt tggaatcgat tttccgcatc ttcgatatcc gttatcgtaa
      781 atcgcaaatt ttcagcgtaa ttttgatggg gattagtcca gagaaacggc cgataggatt
      841 gaagggaaag aatttccgga tatttgataa tcaattcatc gatattcatt tcactttctt
      901 tttatcatcc ctttcgtaat tcgactttct cataattata ttgagttatc gttcgatatt
      961 tctgaacctt gttatcaaga gggaagactc cttcctctgt catgattcag cgattatttc
     1021 ttttcaaatc aggacacttc caataaaatt tttttattcg gattgttctg atgaacctgt
     1081 ctccaaagga aaaataacga atgaaggtaa aagctttaaa ggcggctttc ccgtttacga
     1141 tccccgtaat gatcggttat ctatttctcg ggattggatt cggtatttta ttgaccggaa
     1201 agggatatcc ctggtggtta tcacctttaa tggccctttt tatttatgcc ggctccatgc
     1261 aatttgtcgc gattgatatc ttttcaaaag catttaaccc tttaaatgct ttgatattaa
     1321 ctttgaccgt caatgccaga cacctttttt atggtttgtc aatgttaaca aagtttaagg
     1381 ctacggatca aaaaaagcct tacttgattt ttgcattaac cgatgagact tattctcttc
     1441 tgtgctcaac agagccgcca cagggaatcg atcgaaattg gttttatttc tttatcgccc
     1501 tgctggacca gatctactgg gtcaccggtt gtactctcgg ttcggtcgcg ggatcactgg
     1561 tgaaattcaa tacgacgggg attgactttg taatgaccgc tttgtttgtt gtcattatgg
     1621 tcgaacaatg ggaaagttca aaaaatcatt tgcccgtttt aatcggaatc ggagtttcgg
     1681 cgatctgttt gcttctgttc ggaaaagatt tattcatcct tccctccatg atcagtattc
     1741 ttgccattgt gacgattttc aggaaatcga ttgagagaag gaatgtcata tgacgatccg
     1801 acaaattctt attaccgtct cggtgattgt tttcggcacc atcctcactc gttctcttcc
     1861 attctttatt tttccggatt ctaaggaatc acccgactac attaaatatc ccggccaggt
     1921 ccttccatat gcgattattg gaatgcttgt agtctattgt ctaaagaatg taaatctatt
     1981 gaattttccg tttggaattc cggaaggaat tgctttgatt tacgtcgtgc ttattcatca
     2041 tcgccgacac aatttgttgt tgagtatcgg gggaggtaca attttatata tggtaatggt
     2101 acagtttctg tttccagtct gatctctttc atgaaccgaa actgcggttc gttcgaatga
     2161 gtcataaaaa atgaaaatag tggaaagtgg tatactttta gcacaaaatt aaacacatcg
     2221 ggagttggat atgaaagtct ccgtatcaca aacgaatctc gcacaggctt taagtatcgt
     2281 ttctcatgca gtcgcaacca aaagtacact ggctatattg gtcaacattt tgatcacgac
     2341 cgaagacggc cagctgagac tttcagccac caatctggaa atcggtatca attatcgcat
     2401 accggctgac atcatcgaag aaggtgccgt gaccgttccg gcaaaactga tgatcgattt
     2461 gatcaatacg cttccgaatg ataccgtata tctggaattg gataccaaaa cccaaacttt
     2521 atcgattcgt tgcctgcaag tgattacgga aatcaaaggg attgatgcaa acgaattccc
     2581 accgatgccg gtattcgaag aagataacgc catgacattt gacggttccg aacttcgaaa
     2641 aatgatacaa caagtcgcct ttgcggcttc caccgatgac gcgcgcccga ttttgcaggg
     2701 aatttacctg accgcagccg gaaaaacaat gacaatggtt gcgactgacg gctttcgaat
     2761 cgcaattcgc aattcgacgt tagaggacag ttttgcccag ccattatcgg ctatcatccc
     2821 cgcccgggct ttggttgagc tggttcggat taccaacgat tcggataaac agattatgat
     2881 gacgatctca gccgggcgga atcaggttat attccatctg ccgcagcttg atttggcatc
     2941 acagacgatt tccggaaatt atgtggacta taaagccatt gttccgaaat cattccagac
     3001 taccacggtc gtttcgacgg ctgctttcca aaaaacctgc agtcaagcga tgattattgc
     3061 ccgcgattcc aaatatctga cacgttttaa cattatgggg caggaaaacg gaatgggaaa
     3121 gattcaagtc accgcccaat cggaagaatc cggtttttat gaaaattata ttgacgcgaa
     3181 tgttgacggg cccgatattc agattgcatt caacgt
//
LOCUS       DAOMIV010000218         3210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_31992_length_3210_cov_3.892552, whole genome shotgun sequence.
ACCESSION   DAOMIV010000218 DAOMIV010000000
VERSION     DAOMIV010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31992_length_3210_cov_3.892552"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PKV59_05885"
     CDS             <1..577
                     /locus_tag="PKV59_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46319.1"
                     /translation="GKLPTDSCPDTVSEVFLAGNEPTEYDDLYVRIPINRNNLKMATA
                     YTKFADIEQKVYMNLPKEANDWAIDNHIETMPAEYDPIPEMIASPVKIYQPINFSIFK
                     KTESQNDPLEIKAEINIPEEILSVQVVLGKGFYPDRWYELNNSSEINNGRWTLAKSDW
                     SALDPALYDLRVSVITKDLKYYRADQYFSVQ"
     gene            complement(699..1199)
                     /locus_tag="PKV59_05890"
     CDS             complement(699..1199)
                     /locus_tag="PKV59_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQP46320.1"
                     /translation="MSVLDVIRKRRSIRKFESRNIEQEKLDSIIEAGRLAPSASNQQS
                     WKFVLVTDKRNLIELAHACHDQMFVAEAPAAVVVCSEDNRMMSCGQSAATIDCSIALS
                     FMILEATDLGIGACWLGSFEPEQVKEVLDLPKTYTVVAVSPLGYPAEIPNPRPRKSVD
                     QILIRR"
     gene            complement(1345..1500)
                     /locus_tag="PKV59_05895"
     CDS             complement(1345..1500)
                     /locus_tag="PKV59_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46321.1"
                     /translation="MEINKEFLFKDKKPNKPGKSDDTKKPAAAPKPAAPAKGPQKPGN
                     EPGKKGK"
     gene            complement(1774..2811)
                     /locus_tag="PKV59_05900"
     CDS             complement(1774..2811)
                     /locus_tag="PKV59_05900"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQP46322.1"
                     /translation="MKFICFGAGAIGSYIGGSLQSAGHDVTFIERKESLPRIRSNGIQ
                     IEDVKGNILKIESPHVTDDALEAVKECQFDAGVIAVKSFDTEKLMKSLIEVIGLLPPF
                     ISLQNGVENEALLASYLGKAKVVPASVCTAISRKQDGLIKVEKLRGIGITAGHFLSED
                     IYKAFRSANLKPALIADGTAMKWSKMISNLLANASSAILDMTPAEIFSDPDGFRLEIQ
                     QIREAVRVMDAYAIPVINIPGIPVKTLVWAVQNLPEKLLRPVLINAVGGGRGEKMPSF
                     YIDLHSGQSQNEVEYLNGAVVRFAREKAIHVPVNSGYYRILTALVKKELPLETYRHQP
                     RKLAQDIFANE"
     gene            complement(2808..>3210)
                     /locus_tag="PKV59_05905"
     CDS             complement(2808..>3210)
                     /locus_tag="PKV59_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46323.1"
                     /translation="LRSLPLILDGTLSAKPQSDEGVTYAPLISKEDGKLDFRLPALQS
                     ERKIRAYHPWPTAYFFHNGTNIKVYKAHVVEISAMDTVPGKTFVYRGLPAVMTGEDAL
                     VFDEIQSAGRKVLSGKEFLSGYRNWEEGFAE"
BASE COUNT          887 a          736 c          625 g          962 t
ORIGIN      
        1 gggaaagctg cctaccgaca gttgccccga tacggtaagt gaggtattct tggcgggtaa
       61 tgagccaacg gaatatgatg atctatacgt caggatcccc attaatcgaa ataatttaaa
      121 aatggccacc gcctatacaa aatttgccga cattgagcaa aaagtctata tgaatctccc
      181 caaggaggca aatgattggg cgattgataa ccatatcgaa acaatgccgg cagaatatga
      241 ccccatcccg gaaatgattg catctcccgt aaaaatttat caaccgatca atttcagcat
      301 attcaagaaa accgaaagtc agaacgatcc gttggaaatc aaagcggaga taaacattcc
      361 cgaagaaatt ctctctgttc aggttgtcct cgggaagggc ttttatccgg acagatggta
      421 cgaattaaac aactcttccg aaattaataa cggacgttgg actttggcga aatcggattg
      481 gtctgcgttg gatccggctt tatatgattt gcgagtttcc gttattacga aagatctgaa
      541 atattatcgg gcggatcaat atttcagtgt gcaatagatt gaaagcattt tcgataaaat
      601 catacaaggt gattaatctt ttttgaaaaa aaagagagtt ggtaccctct ttttttgact
      661 tcattttgta atttgatcga ttccgttctg agccgctttt accggcgtat taaaatctga
      721 tcgactgatt ttctgggacg gggattcggt atttcagcgg gatagccaag cggcgaaact
      781 gccacaaccg tatatgtctt cggtaaatca agcacttctt tcacctgctc cggttcaaaa
      841 gaacccaacc aacaagctcc tatccctaaa tcggtcgctt ccaaaatcat aaaagacagc
      901 gcaatagagc aatcgattgt cgcagcggat tgaccgcagc tcatcattcg attatcttcc
      961 gaacaaacaa caaccgccgc gggagcttcc gccacaaaca tttggtcatg gcaagcgtga
     1021 gccagttcga ttaaattcct tttatctgtt actaaaacaa atttccaact ttgctgatta
     1081 cttgcggaag gtgccaatcg cccggcttcg ataatagaat ccagtttctc ttgttcgata
     1141 tttcgacttt cgaatttcct gatacttctt cgttttctga tgacatccaa tacgctcata
     1201 ggatccctcc cttttctgat aagttcaata attcgcttta cataattatt ctacagactt
     1261 atcagggttc gatcaagtga aattaaataa agatatggga ggcttctccc atatcttaaa
     1321 attcagataa acttttgttc gggtttattt cccttttttg cccggctcat tccccggttt
     1381 ttggggacct ttcgcaggcg cagccggctt tggtgcagcc gccggttttt tcgtatcatc
     1441 tgattttccg ggtttgttcg gttttttatc cttgaataaa aattctttat tgatttccat
     1501 atcatcctcg attcattcat tcaaattttt aggaaaggtt tcccatgaat ttcgacccgc
     1561 tgtaacgatt ccttccggaa ttactgcgcc gtccgaggcg acttgattgc ttttttggaa
     1621 tgatcccgta tgtattttcc gcgaaaaatc ctccttctct aattaactga aaatgagtat
     1681 agcacttctg tcaatagttg aaatagaaat taatacgatt tatttaaacc ttttgccttg
     1741 aatttaatgg aaattttatg taagcggttc aaattattca tttgcaaata tatcttgggc
     1801 aagtttacga ggctgatgcc gatatgtctc caaaggaagt tcttttttta ccaatgcggt
     1861 caaaatacga taatagccgc tgttgaccgg cacatggatc gccttttccc ttgcgaatct
     1921 cacgacagct ccgttaaggt attcgacttc gttttgagat tgtccggaat gcaaatcgat
     1981 ataaaaagac ggcattttct ctccacgccc tcctcctacc gcgttgatta gaacgggacg
     2041 taataatttc tcgggcagat tttgaacagc ccatactaat gtttttaccg gtatccccgg
     2101 aatattgatc accggaatag cgtaagcatc cattacccgg accgcttccc gaatctgttg
     2161 gatttccaac cggaatccgt ccgggtcgga aaatatttcg gcaggtgtca tatccaaaat
     2221 tgcggaggaa gcattagcca gcaagtttga aatcattttc gaccatttca tcgcagtccc
     2281 gtcagctatc aaagccggtt tcaaatttgc cgatcggaaa gccttataaa tgtcctctga
     2341 aagaaaatgt ccggcggtta ttcctattcc acgcagtttc tcgactttga tcaaaccgtc
     2401 ttgttttctg ctgatggcgg tacaaacgga tgccggtaca acctttgctt ttcccaaata
     2461 ggaagcaaga agagcttcgt tttcaacacc gttctgaagg ctgataaaag gaggcagcaa
     2521 gccaatgact tcgatcagcg atttcattaa cttttccgtg tcaaaagatt tcaccgcaat
     2581 aacaccggca tcaaattgac actcttttac ggcttctaag gcatcatcgg tgacatgagg
     2641 cgattcaatc ttaagaatat tcccttttac gtcttcgatt tgtattccat ttgatcgaat
     2701 tcgcggcaaa ctttcttttc gttcgataaa ggtaacatca tgacctgccg attgaagact
     2761 tcccccgata taagagccga tcgctcccgc tccaaaacaa ataaatttca ttctgcaaat
     2821 ccttcttccc aatttcgata tcccgaaaga aattcctttc ccgaaagcac tttccttcct
     2881 gcactttgaa tctcatcgaa caccaacgca tcctcaccgg tcattacagc cggcaagccc
     2941 cgataaacaa aagtctttcc cggaaccgta tccatcgctg aaatttcaac gacatgggct
     3001 ttgtaaactt taatatttgt tccgttatga aaaaaatatg ctgtcggcca cggatgatag
     3061 gcacgaattt ttctttccga ttgaagagca ggcaggcgga aatcaagttt tccgtcctct
     3121 ttggatatca acggagcata agtgacgcct tcatctgatt gtggtttcgc cgataatgtt
     3181 ccgtccaaaa tcaatggcaa agatcgaagg
//
LOCUS       DAOMIV010000219         3206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_32053_length_3206_cov_4.994288, whole genome shotgun sequence.
ACCESSION   DAOMIV010000219 DAOMIV010000000
VERSION     DAOMIV010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32053_length_3206_cov_4.994288"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKV59_05910"
     CDS             complement(<1..439)
                     /locus_tag="PKV59_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP46324.1"
                     /translation="MLRIENITKIFEPGTVSEKTALVNLDLTLNEGDFVTVIGGNGAG
                     KSSLLNAISGRFFVNEGAIFIDDIDITYQPEYKRAQWIGRVFQDPMMGTAGNMTIEEN
                     LAIALRRGKTRTLNWGMAKGEHDLFYDQLAELELGLEDRIKTKV"
     gene            complement(454..1392)
                     /locus_tag="PKV59_05915"
     CDS             complement(454..1392)
                     /locus_tag="PKV59_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46325.1"
                     /translation="MLDILINAISLGILWAILAIGVFFTFRILDIADLSCEGVFPLGA
                     AIAARIISDGGDPFLACLAAIGGGAIAGTFTGILHTKLKIPILLAGILSMTALYSINL
                     RILGKANISLLRMNTVFTKFESMLHTGGKASTFILGLLIVAIIIFILWWFLNTELGYS
                     VRATGNNVYMIRAMGVDTDSMFMIGLIIGNALIGLAGAIIAQKQSYADIGMGTGTIVI
                     GLASVIIGEVLFGKKGVLQHMVSIVLGSIVYRIIISLILEIDFEIHLGKIVIPISVEP
                     TDLKLFTSIIVIIALSLPVIKQRRASKKASSMLKSI"
     misc_binding    1527..1767
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1831..>3206
                     /locus_tag="PKV59_05920"
     CDS             1831..>3206
                     /locus_tag="PKV59_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQP46326.1"
                     /translation="MFKPVSSKLNVNEMEEGVLKFWKDENIFKKSTDNREGGREYVFY
                     EGPPTANGKPGVHHVISRSIKDVILRYRTMQGCHIIRRGGWDTHGLPVEIEVEKKLGF
                     TNKQQIEEYGIAKFNALCKESAFSNIKEWERMTERVAYWVDFDTAYITYKDEYIESEW
                     QILKNFFERDLLYQGFKVVPYCPRCGTPLADHEVAQGYHDVEDPSIYIRFRLKNQPDT
                     DLLVWTTTPWTLTANVGIAAHPDVDYVKIERPLPDGTSEKLIFAESLVPVVLPGEEVK
                     ILEHYKGKDLKGWEYEPLFHFLPLEKPAHRVVMADYVSVEDGTGLVHIAPSFGAEDME
                     VGKVEDLPVLMTVGPDGRFIPEVTEWAGMFVKDADPLIIKELSARGRMLKHGKIKHSY
                     PFCWRCNTPLLYYARPTWFIRTSQFKDRLVELNEKINWYPSHIKNGRFGNWLENNIDW
                     ALGRNRYWG"
BASE COUNT          906 a          698 c          753 g          849 t
ORIGIN      
        1 cgactttcgt tttaattcga tcttcgagtc ccaattcgag ttcggcaagc tgatcataaa
       61 agaggtcatg ctcgcctttt gccatgcccc aattcagggt tcgggtcttc ccgcggcgta
      121 aagcaatcgc aagattttct tcgatcgtca tattgccggc ggtccccatc atcggatcct
      181 gaaaaactct gccgatccat tgtgcccgtt tatattccgg ttgataggta atatcgatat
      241 catcgataaa aatagcccct tcattgacga aaaaacgacc ggaaatcgca tttaataagc
      301 tggattttcc ggcgccgttt ccgccgatca ccgtgacgaa atctccttca ttcaaggtta
      361 aatcaagatt caccagtgcg gttttttcac tcaccgttcc cggttcgaat atttttgtaa
      421 tattttcaat tctgagcatt tcttctttcc tgatcagatc gacttaagca tcgatgaggc
      481 ttttttggaa gccctgcgct gtttgataac cggcagagac aaagcgataa tcacaatgat
      541 cgatgtgaaa agtttcaggt ctgtcggttc aacggagatc ggaattacta tcttacccag
      601 atgaatttca aagtcgattt caagaatcag ggagatgata attcggtaaa caatactgcc
      661 gagaacaatt gagaccatat gctgaagaac gccttttttc ccgaacagta cttccccgat
      721 aattaccgaa gccaagccga tcacaattgt gccggttccc ataccgatat ccgcataact
      781 ctgtttttga gcaatgattg cgccggctaa gccgatcaac gcattaccga tgatcaaacc
      841 gatcatgaac atactgtcgg tatcgacgcc catcgcccga atcatataca cattattgcc
      901 cgttgcgcga acggaataac ccaattcggt attcaggaac caccacagaa taaatataat
      961 tatagcgaca attaataatc ccaaaataaa agtggatgct ttaccgcccg tatgtaacat
     1021 actttcgaat tttgtgaaga ccgtattcat ccgtaagagg gaaatattcg ctttgcccaa
     1081 aattctcaga ttgatggaat agagggctgt catggaaagg atgccggcta aaaggatcgg
     1141 aattttaagc ttggtatgca ggattccggt gaatgtcccg gcaattgccc cgccgccgat
     1201 tgccgccaaa caagccaaaa aaggatctcc gccatccgag atgatccggg ctgctatcgc
     1261 cgcaccgagc gggaatactc cttcgcaaga caaatcggca atatccagaa ttctgaaagt
     1321 aaagaaaact ccgattgcca atatcgccca tagaattccc aatgatattg cattaatcag
     1381 tatatccagc acaattccct cggtattttt aaatcaagtg taattctatc atgcgatttt
     1441 ataatgaatt tgtcatgatg attgatataa ttacactcta aattcggctg tttgaccgtt
     1501 tgtatgaaac atggttaaat agcatacgaa atgcgttgac gaaaagagta ccgtcagtag
     1561 gaatccacag cgaatccggg agggtgaaag ccggatgatc aagctggcga ggaaaatcgt
     1621 ttctgagcaa tgatccgaaa ctgtattccg aggaggaagc gggagtaagt gattcgggat
     1681 aatccgccgt tatcggatag agagtgttca tattcatgca gaatatgaaa acaagggtgg
     1741 taccgcggaa tttaccccgt ccctttcggg acggtttttt tattaaagat atctctcagc
     1801 cgatatggct ggaaacaaat aaggagtctt atgtttaaac cggtttcatc caaacttaat
     1861 gttaatgaaa tggaagaagg ggttctgaag ttttggaaag atgaaaatat tttcaaaaaa
     1921 tcaaccgata atcgcgaagg cggccgtgag tatgtgttct atgaaggtcc gcctaccgca
     1981 aacggaaaac ccggtgtaca tcatgtaatt tctcgctcca ttaaggatgt aatccttcgc
     2041 tatcgtacga tgcaagggtg tcatattatc cgccgcggcg gttgggatac acacggcttg
     2101 ccggtcgaaa ttgaagtgga aaagaagttg ggatttacca acaaacagca aatcgaagag
     2161 tacggcatcg cgaaattcaa cgctctttgt aaagaatcgg ctttttcgaa tatcaaagag
     2221 tgggaacgca tgacggagcg ggtcgcctat tgggttgatt ttgatacggc ttatatcact
     2281 tacaaggatg agtatatcga atccgaatgg caaatcctga agaatttctt tgaaagagat
     2341 ttgctttatc aggggtttaa agtcgtccct tattgcccgc gctgcggaac tcccctggcg
     2401 gatcatgaag tcgctcaagg ctaccatgac gtggaagatc cttctatcta tattcgattt
     2461 cgcttaaaga accaaccgga caccgatttg ctggtgtgga caaccactcc gtggacgctg
     2521 acggcgaatg tcggaattgc agctcatccg gatgttgatt acgtcaaaat cgagcggccg
     2581 ttgcctgacg gtacttccga aaagttgatt tttgccgaat ctctcgtccc tgttgtccta
     2641 ccgggtgaag aggttaagat tctcgaacac tataaaggga aggatttgaa aggctgggaa
     2701 tacgaaccgc tttttcattt cctgcctttg gagaagcccg cacatcgcgt ggtgatggct
     2761 gactatgttt ctgtggaaga cggaacgggg ctggtccata ttgctccttc attcggtgcg
     2821 gaagacatgg aagtcggaaa agttgaagat ttaccggttt tgatgacggt cggaccggac
     2881 gggcggttta tcccggaagt gaccgagtgg gcggggatgt tcgtgaaaga tgcggatccg
     2941 ctgattatca aagaactaag cgcacgcggt cgaatgctga agcacggaaa aatcaaacac
     3001 agctatcctt tctgttggcg ctgcaatacg cctttgcttt attatgcccg gccgacatgg
     3061 tttatccgaa ccagccagtt taaagatcgg ctggttgaat tgaacgaaaa aatcaactgg
     3121 tacccgtctc atattaaaaa cgggcgattc ggaaattggc ttgaaaataa tatcgattgg
     3181 gcgttgggaa gaaaccgata ttgggg
//
LOCUS       DAOMIV010000220         3181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_32383_length_3181_cov_4.240563, whole genome shotgun sequence.
ACCESSION   DAOMIV010000220 DAOMIV010000000
VERSION     DAOMIV010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32383_length_3181_cov_4.240563"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..462
                     /locus_tag="PKV59_05925"
     CDS             49..462
                     /locus_tag="PKV59_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HQP46327.1"
                     /translation="MRSTKSNPMSLIRFEVYQCETCGNQVIKSIDGGGELVCCEKPMK
                     KLEVNSSDGAAEKHVPVYEYLGSREMLVRVGSLDHPMTEEHYIGWIAVVYQNTVIWEK
                     LKPGMAPQIIANVESGYNGEIYAWCNLHGLWMAHF"
     gene            490..1029
                     /locus_tag="PKV59_05930"
     CDS             490..1029
                     /locus_tag="PKV59_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQP46328.1"
                     /translation="MELKGSQTEKNLEAAFAGESKATNKYSYYASQAKKDGFEQIGGI
                     FSETSGNEREHAKLWFKYLHGGEIPSTPANLLDAAAGEHYEWTEMYKGFAETARQEGF
                     SEIAARFELVAKVEKGHEERYRKLAANIDNKKVFEKDEEVVWICRNCGYLHYGKKAPE
                     KCPTCGHPQSFFEVHCENY"
     gene            1135..1626
                     /locus_tag="PKV59_05935"
     CDS             1135..1626
                     /locus_tag="PKV59_05935"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQP46329.1"
                     /translation="MKACCFYESPVGMLGIAENGTAITGLFFGNTMPSGEWQLRNTPL
                     LAQAVDQLEAYFSGERTAFDLPLDAEGTVFQRSVWEALRRIPYGETRSYGQIAEAIGK
                     PKAGRAVGMANHRNPIAIIIPCHRVIGSDGSLVGYGGGLNVKTFLLRLEQGISERDKN
                     NLR"
     gene            1738..2760
                     /locus_tag="PKV59_05940"
     CDS             1738..2760
                     /locus_tag="PKV59_05940"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HQP46330.1"
                     /translation="MQHEVTVIQDLLDSNGHLREPGWAKKPVFNYRRKDIKASRFRIK
                     EWDYYLILNDHHAIALTLADNSYMGMISASVLDFDSGTEHTGSIMTAFPMGKFKMPET
                     ADESHCHFENKRAKVDFLATEGTRTIFFEYKNFWEGKDLHAEFTLTQPFEDRIVIATP
                     WKEDPKAFYYNQKINCMPAKGFYTLGDHRVDFHESDTFGTLDWGRGVWTRDNTWYWGS
                     GNGWVEGGRFGFNFGYGFGDTSAATENALLYEGMLTKVDEVKFLPSDPDHLLMPWRIE
                     SNDDRVDLDFSPILDRAAAINALILSTDQHQVFGRVSGNVLLENNRRLDIRDLTCFFE
                     IVHNVY"
     gene            complement(2863..>3181)
                     /locus_tag="PKV59_05945"
     CDS             complement(2863..>3181)
                     /locus_tag="PKV59_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HQP46331.1"
                     /translation="NNVTRYNVFAREPVNPGPGGKTTLVFTTRHQPGALLNALRVFAE
                     RKIDLTKIESRPLPDQPYEYMFYADISGSISDPNVKEAIDELEQYHAPWIRVLGSYMS
                     IKD"
BASE COUNT          855 a          623 c          873 g          830 t
ORIGIN      
        1 ttccggatga ttgggtttgc ccggtttgcg ggttagcgaa agatgaattt gaggagtacg
       61 aagagtaatc cgatgtcatt gattcgattc gaagtttacc aatgtgagac ctgcggaaat
      121 caggttatta aaagcattga cggcggtggc gaactggttt gttgcgaaaa accgatgaaa
      181 aagctcgagg taaattcgag cgatggcgcc gcggaaaagc atgttccggt ttacgaatat
      241 ttgggcagtc gggaaatgct ggtgcgggtg ggaagcctcg atcatccgat gacggaagag
      301 cactatatcg gatggatcgc tgtggtttat caaaataccg tcatctggga aaagctgaaa
      361 cccggaatgg ccccgcaaat tattgccaat gtggaatcgg gttacaacgg cgaaatttat
      421 gcttggtgta acctgcatgg tttatggatg gctcattttt agccgacata tccgaaagaa
      481 aggatcatta tggaattaaa aggatcacaa acagaaaaga accttgaagc ggcatttgcc
      541 ggtgaatcca aggcgacgaa taaatattct tattatgctt cacaggcgaa aaaagacgga
      601 tttgaacaga tcggcggcat tttttcggaa acctccggaa acgaacgcga acatgccaaa
      661 ttatggttta aatatctgca cggcggagaa attccgagta ctccggcaaa tttgttggat
      721 gcggctgccg gtgagcatta tgaatggacg gaaatgtaca aaggtttcgc cgaaactgcc
      781 cgacaggaag ggttctcgga aatcgcagcc cgttttgagc tggtagcaaa ggtcgaaaaa
      841 ggccacgagg aacgatatcg aaagctggcg gcaaatatag acaataagaa agttttcgag
      901 aaagatgaag aagtggtttg gatttgccgg aattgcggat atctgcatta cggaaagaaa
      961 gctccggaaa aatgccccac ctgcggtcat ccgcagtctt tctttgaagt tcattgcgag
     1021 aattattaat tttcgccgat tctttgaaat ggaaatcaaa aaaactgccg gaaggcagtt
     1081 tttttggtat atttgaaata gggataatca tcacggaatg aaaaggggct gataatgaaa
     1141 gcctgttgtt tttatgaatc accggtcggc atgttgggga tcgcggagaa tgggacagcg
     1201 attaccggcc tgttctttgg gaatacaatg ccttccggcg aatggcagct ccggaatacg
     1261 ccgttgttgg cgcaagccgt cgatcagctc gaggcttatt tttccggtga acgaacagct
     1321 tttgatcttc cgctggatgc ggagggaacg gtatttcaac gttcggtctg ggaagcgctg
     1381 cggaggatcc cttacgggga gacccgatcc tacggtcaga tcgccgaagc gatcggaaaa
     1441 ccgaaagccg gcagagcagt ggggatggcg aatcatcgaa atccgattgc aatcattatt
     1501 ccctgtcatc gcgtgatcgg ttccgacgga tccttggtgg gatatggcgg gggactcaac
     1561 gtgaaaacgt ttttattgcg attggaacaa ggaatcagcg aaagggacaa aaacaatttg
     1621 aggtaaagaa agaaaatcgc ctcaggggtt ctttaatcgc ttcttttacc ggatcgattt
     1681 tggaaaattc aatcgactgc aatctttcaa aaatcgatac cgatcaggag gtttcctatg
     1741 caacatgaag taacggtcat tcaggatttg ttagattcaa acggtcatct gcgggaaccg
     1801 gggtgggcga aaaaaccggt ttttaattat cggcgaaaag acatcaaggc aagtcgcttt
     1861 cgaattaagg agtgggatta ttatctgatc ttaaatgatc atcatgcgat tgcgctgact
     1921 ttggccgaca acagctatat ggggatgatt tcggcttcgg tacttgattt tgattccggg
     1981 acggagcata ccggttcgat aatgaccgct ttcccgatgg gaaaatttaa gatgccggaa
     2041 acggctgacg aaagccactg tcattttgaa aataagcggg cgaaagtgga ttttctcgcc
     2101 accgaaggga cacgaacgat ctttttcgaa tataaaaatt tttgggaagg aaaagatttg
     2161 catgccgagt tcacattaac tcagccgttt gaagatcgga ttgtcatcgc aactccttgg
     2221 aaagaggatc cgaaggcttt ttattataat cagaagatca attgtatgcc ggcgaagggt
     2281 ttttatacac tcggagatca ccgggttgat tttcacgaat cggacacttt cggcacactt
     2341 gattggggac gcggtgtttg gacgcgcgat aatacctggt attggggctc cggaaacggt
     2401 tgggtggaag gcggccggtt tggattcaat tttggttacg ggttcggcga taccagcgcc
     2461 gcgacggaaa acgctctgtt gtatgaaggg atgttgacca aggtggatga agtcaaattt
     2521 ttgccgtccg atccggatca tcttctaatg ccttggcgga tcgaatccaa tgatgatcgg
     2581 gtggatctgg atttttcccc gatattggat cgggcagctg cgatcaatgc tctgattttg
     2641 tccacggatc aacatcaggt attcgggcgt gtctccggaa atgttctgtt ggagaataac
     2701 cgccgtttgg atatacgcga tctgacttgc tttttcgaga tcgtacataa tgtctattga
     2761 tcgaatctgt tcggcgttct tttggagatg acgaaaataa aacccgggtt cgaaaggctg
     2821 cccgggtttt atgactgttc cgtttgattg cgtaaaagtt gcctaatctt ttatggacat
     2881 ataactgccg agaacccgaa tccacggcgc atgatattgt tcgagttcat cgatggcctc
     2941 tttgacattc ggatcggaga ttgagcctga gatatcggca taaaacatat attcgtaagg
     3001 ttgatcgggc agcggtctgg attcgatttt ggtcaggtcg atcttgcgtt ccgcaaaaac
     3061 gcgcaatgcg ttgagcagtg caccgggctg atggcgggtg gtgaaaacca acgttgtctt
     3121 tcctccgggc cctggattga ccggttcgcg cgcaaaaaca ttatatcggg ttacattatt
     3181 c
//
LOCUS       DAOMIV010000221         3174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_32495_length_3174_cov_4.486694, whole genome shotgun sequence.
ACCESSION   DAOMIV010000221 DAOMIV010000000
VERSION     DAOMIV010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32495_length_3174_cov_4.486694"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PKV59_05950"
     CDS             <1..553
                     /locus_tag="PKV59_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQP46332.1"
                     /translation="PLTKVTISPEREALYEATKEAWLTVNPVEIKSTQSDHATPVPQD
                     QAPKAVESESIVLANTKPAAVTEAPQGFYQLQAGETPKCIARRFNVDWLKLYSMNNIS
                     FENENDIQPGTVLVIPQNSVWNEKHGPRASLPHPTDYNVQAGDTLNSIACQFGDVTPE
                     QIALENGITSQEQLIPGLSLHIP"
     gene            566..1672
                     /gene="tgt"
                     /locus_tag="PKV59_05955"
     CDS             566..1672
                     /gene="tgt"
                     /locus_tag="PKV59_05955"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQP46333.1"
                     /translation="MTEPETEKSWLETPHGRLDYPVYLPDATYGMVRGVDAVDLEQAG
                     VQGLVMNTFHLMQKPGSLTVQSLGGIHTMSGWSKPIITDSGGFQAYSLIRENAKFGKI
                     NENGITITPEGSKRKLLLSPEKTVQLQMSYGSDVVICLDQCTHPDDSDQVQRESVRRT
                     IEWAKRCKHEFTKRLIHTEKDTKPPKIFGVVQGGNSFELRKTCADALLEIGFDGFGFG
                     GWPLDGEGNLVEDILQYTRELIPAEYPLHALGIGHPENVVRTFRLGYDIFDCAMPTRD
                     ARHGRIYAFREDPPQLEGKWLRYLYIDDDKYIRSSQPLSPGCDCLTCTRYSTGYLHHL
                     FKMNDSLYPRLATIHNIRILTRLTELLRCEKYGK"
     gene            1662..2477
                     /locus_tag="PKV59_05960"
     CDS             1662..2477
                     /locus_tag="PKV59_05960"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46334.1"
                     /translation="MENDSSVLQIAAEISAGKNFREIDPGLIRHLIKTEMQPNESEKE
                     TVKRVRSKLHQISNAYREGASNYQDLITAIRHSDNSLISLEAAKPLAIELMKSHASTR
                     ERIAILDVFYSQIFAALPPIRSVLDLACGLNPLAIPWMKLADHFTYEAYDIYRDIALL
                     LNVFFEKLNIRGKADSMNLLEEFPQSECDLTFLLKTIPCLEQVDKNAGTRILNQIRSP
                     FAVVSFPIFSLSGRQKGMKVNYEKHFLEIYDPQRWELERMLFPNELVFLMRRK"
     gene            2474..>3174
                     /locus_tag="PKV59_05965"
     CDS             2474..>3174
                     /locus_tag="PKV59_05965"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46335.1"
                     /translation="MNDRKETDDTVDQIVRKILSSPKYRELGIPKESVEDIVKREVSR
                     QTDIKVAEKKAREKLHQVVAPYLGDPDYRSAGTALTQIHRDDREGLKAWCLEMLRSHS
                     STRERLPNLELFYRTIFEFTGKPNTVLDLACGMNPFAFPWMGLESSVRYYAYDLHTPR
                     VNLINHFFDAWGLQPLATVTDVLVSVPQTPADVAFFFKEAHRFESRRKGSNRAFFQQL
                     SVKWIVVTLPAENLTG"
BASE COUNT          870 a          781 c          758 g          765 t
ORIGIN      
        1 acctttgacg aaagtaacca tcagtcccga aagagaagcc ctttacgaag cgacaaaaga
       61 agcttggctg acggtaaatc ccgttgaaat aaagagcacc caatccgacc atgccacacc
      121 ggttccgcaa gaccaagcac cgaaagccgt tgagtcagag tcgatcgttc tggcaaatac
      181 caaaccggct gcggtaacgg aagcgccgca gggattttat caattgcagg ccggcgaaac
      241 accgaaatgt atcgcccgcc gctttaatgt tgattggctg aagctgtact cgatgaataa
      301 catcagtttt gaaaatgaaa acgatattca accgggaacc gttttagtca ttccgcaaaa
      361 ttccgtttgg aatgaaaaac acggacctcg ggcttcgttg cctcatccca ccgactacaa
      421 tgtccaagcc ggcgacacac tgaacagtat tgcctgccaa ttcggagatg tgacccccga
      481 acaaatagcg cttgaaaacg gcattacttc acaagaacaa ttaattccgg gattgagcct
      541 gcatattccc tgatgcccga tcgaaatgac tgaaccggaa accgaaaaat cctggcttga
      601 aactccccat gggcggttgg attatcctgt ctatctgccg gatgcaacct acggaatggt
      661 gcggggagtc gatgcggtag atctggagca agccggtgta caggggctcg tgatgaatac
      721 atttcatctg atgcagaagc ccggcagtct gaccgttcag tctcttggcg gcattcacac
      781 aatgagcggt tggtccaagc cgatcatcac cgactccggc ggttttcagg catattcact
      841 cattcgcgaa aatgccaagt tcgggaaaat aaacgaaaac ggaatcacga tcacaccgga
      901 agggtccaaa cggaaactcc tgctcagccc ggaaaaaacc gttcaattgc agatgtcata
      961 cggatcggac gtggtaatct gcctggatca atgtacccat cccgatgatt cggaccaagt
     1021 gcagcgggaa tccgtccgcc gcacgatcga atgggcaaaa cgctgtaaac atgaatttac
     1081 caaacgctta atacacaccg aaaaagacac aaaaccaccg aaaatctttg gggtggttca
     1141 gggtgggaac tctttcgaat tgcggaaaac ctgtgccgac gcattgcttg aaattggctt
     1201 tgacggcttc ggattcggcg gttggccgct ggacggagaa ggaaatctgg ttgaggatat
     1261 tttgcaatac acgcgggaat taattcccgc tgaatatccg ctccatgcgt tgggaatcgg
     1321 acacccggaa aacgtcgtcc ggacttttcg attgggatat gatatcttcg attgtgcaat
     1381 gcccacacgg gacgcccgcc atggccgaat ttatgctttt cgcgaggatc cgccgcaatt
     1441 ggaaggaaaa tggctgcgct atctttatat cgatgatgat aaatatatcc gctcatccca
     1501 acccctttct ccggggtgcg actgcctgac ttgtacgcgc tactcgaccg gatatttgca
     1561 tcatttgttc aagatgaatg acagcctcta tcctagatta gccaccattc ataatattcg
     1621 aatccttacc cgattgacag agctgctgcg ttgtgagaaa tatggaaaat gattcatccg
     1681 tccttcaaat cgccgccgag atatccgccg gcaagaactt cagagagatc gatcctggct
     1741 tgatccgtca tttgataaag acggaaatgc aacccaatga atccgaaaag gaaaccgtca
     1801 aacgggtaag atcgaaactt catcaaattt ccaacgctta tcgggaaggg gcaagcaact
     1861 atcaagacct tattacagcg atccggcact ccgataattc tttgatctct ttggaagccg
     1921 ccaagccttt agcgatcgag ctgatgaaat cgcatgcctc gacccgtgag agaatcgcaa
     1981 tattggatgt cttttattct caaatatttg ctgcgctgcc accgatccga tccgtattgg
     2041 atctggcttg cggattgaat ccgttagcca tcccttggat gaaattagcc gatcatttta
     2101 cctatgaagc ttatgatatc tatcgggaca tagcgctgtt actgaatgtt ttttttgaaa
     2161 agctcaacat tcggggaaaa gccgattcga tgaatctgct ggaagaattt cctcaaagcg
     2221 aatgtgatct gacgtttctg ttgaaaacca tcccctgtct ggagcaagtc gataagaatg
     2281 ccggaacccg tatcctgaat caaattcgtt ctccctttgc ggtcgtcagt tttcccattt
     2341 tcagcctgag cggcagacaa aaaggaatga aagttaatta cgaaaagcat tttttggaaa
     2401 tatacgaccc gcaacgttgg gaacttgaaa gaatgctgtt ccccaatgag ttggttttcc
     2461 tgatgagaag aaaatgaacg atcgcaaaga aacagatgac acggtcgatc aaattgtccg
     2521 caagattctc tcatcaccaa agtatcggga attgggaatt ccgaaagaaa gtgtcgaaga
     2581 tattgtcaaa cgggaagttt cccgacaaac cgacatcaaa gttgccgaga aaaaagcgcg
     2641 ggagaagctg caccaagtgg tcgcccctta tttgggcgat ccggactatc gctcagccgg
     2701 taccgcattg acgcaaattc atcgtgatga tcgggaaggg ctcaaagcct ggtgcctgga
     2761 aatgctccga tcccattcat ccacccgcga gcggctgccg aatttggagc tgttctatcg
     2821 gactatcttt gaattcaccg gaaaaccgaa tactgttctt gatttggcat gcgggatgaa
     2881 cccctttgct ttcccctgga tgggattgga atcgtccgtc cgctattatg cctatgacct
     2941 gcacactccg cgggtaaatc tgatcaatca cttcttcgac gcatggggat tacaaccgct
     3001 ggcaaccgtg accgatgttt tggtttcggt tccgcaaacg ccggcggatg ttgctttctt
     3061 ttttaaggaa gcgcatcgct tcgaaagtcg tcggaaagga tccaatcgag ctttttttca
     3121 acaactttcg gtaaaatgga ttgtggtcac tctgccggca gaaaacctga ccgg
//
LOCUS       DAOMIV010000222         3174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_32500_length_3174_cov_4.003847, whole genome shotgun sequence.
ACCESSION   DAOMIV010000222 DAOMIV010000000
VERSION     DAOMIV010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32500_length_3174_cov_4.003847"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1105)
                     /locus_tag="PKV59_05970"
     CDS             complement(104..1105)
                     /locus_tag="PKV59_05970"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQP46336.1"
                     /translation="MRTFRMKNRNGIEVGFHSAGASIAYVLTPDRYGNFKNIALTLPE
                     PKDYLNNEIYAGATVGPVAGRIKDGILPVLDQTYHLCRNEGNNTLHGGEKNFAHVIWN
                     MTDFRENEDEASLRFSYTAPDGENGFPGNRKVSVEYILNRLNQLIISYTAETDKPTYL
                     NITNHCYWNLSGDFNRDAYDHILKINADAVYYNDIQFLPTEYVDVKNSPFDFQTPRKI
                     RDAFLNFPDHEQLKLNKGFNHAYLTKGDEAVFLSDNKSGRTLTISTDYPCVVFYSGGF
                     LDQISGNENQRRPAVGSGLAFEAQYLPDAPQLMGDSLPILFPGMIYRKKIVFGFGTK"
     gene            1427..2935
                     /locus_tag="PKV59_05975"
     CDS             1427..2935
                     /locus_tag="PKV59_05975"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQP46337.1"
                     /translation="MDNLQEENILEFSEISKYFPGVKALDNVSFTARSGEVLAFLGEN
                     GAGKSTLLKILNGDYQPDKGKYLINGVEKKFNSPQEAIEEGISVIYQERQILMELTVA
                     ENIFLGRMPVNNFGIINVKKANKMAAAIIKDFGLPINPTTKVKDLSIAFQQMVEIMKA
                     YSRENLKVICFDEPTASLSDSEIDILFGIIRKLKKENKIIIYVSHRLKEIQQITDRVA
                     IFKDGKYITTIKTGEVSEKEMIKMMVGRDLGDIFLNLDRNKEIGDVLLDVKDVSSEFV
                     KECSFQLRKGEVLGFSGLVGAGRTELMRAVIGADKRKGGEISLEGKKIHNSSPKEAMK
                     NGIVLVPEDRKMQGILSNLNVSGNINISLLDKNANKFGIIDSKKELQIAEKCIDEFHI
                     KTPSPLKRIVELSGGNQQKCIVARGISTMPKVLILDEPTKGIDVGTKSEFYQMICAFA
                     KQGLGIILISSELPEVLGLSDRIIVMRSRKIVGEINRETATEALVLSMSMID"
     gene            2971..>3174
                     /locus_tag="PKV59_05980"
     CDS             2971..>3174
                     /locus_tag="PKV59_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46338.1"
                     /translation="MEEEKGIKKTITRITNFIGNDKLVLLIAIIVVFAMFTFLNPNFM
                     SKQNIINLLVAGSLIGLVAIGHTY"
BASE COUNT         1011 a          615 c          667 g          881 t
ORIGIN      
        1 aacggaaccg gccggattcc ctaagaacac aaacataaga agaaccgtaa accccatccg
       61 gttcggcgct tctgattaag ccgagatggg gttttaattt atctcatttc gttccgaaac
      121 cgaaaacaat ctttttccga taaatcatcc ccggaaataa aatgggcaga gagtcaccca
      181 tcaattgggg agcatcgggt agatattgcg cttcaaatgc caatccggag ccaaccgccg
      241 gtctccgctg attttcatta ccggagatct gatccagaaa tccccctgaa taaaaaacga
      301 cacaaggata atcggtggat attgtcaatg ttcttccgct cttgttatcc gataaaaaga
      361 cagcctcatc gccttttgtc aagtaagcat gattaaaacc tttattcagt ttaagttgtt
      421 catgatcggg aaaattcaaa aaagcatcgc ggatttttcg cggagtttgg aaatcaaaag
      481 gggaattttt aacatcgaca tattcggtgg gtaaaaattg aatgtcattg taataaaccg
      541 catcggcatt tattttaaga atatgatcgt aggcatccct attaaaatcc cccgatagat
      601 tccaataaca atgattggtt atattgagat aagtgggttt gtccgtctcg gcggtgtatg
      661 aaataatgag ttgattcagc ctgttcaaga tatattctac cgagactttc cgatttcccg
      721 ggaatccgtt ttcgccatcc ggcgccgtat aggaaaaccg aagcgaagcc tcatcttcat
      781 tttccctaaa atcggtcata ttccaaataa catgggcgaa gttcttttct ccgccgtgca
      841 gagtattgtt tccctcgttt cggcagagat gatacgtctg gtccaaaacc ggaaggatgc
      901 cgtctttgat acgacccgct accgggccga ctgttgcccc ggcatatatt tcattattca
      961 aataatcttt tggttccggt aaagtaagcg caatattttt aaaattaccg taacgatccg
     1021 gtgtcaaaac ataggcaatc gaggcacctg ccgaatggaa tcccacttca attccgtttc
     1081 tgtttttcat tcgaaaagtc cgcataaagt tattccttcc gtcaggatta ttccttttat
     1141 gatccttttt agggagcaca aatgttagta tagaccgaaa taatcaaccg gatatcccga
     1201 tggtaaaaat agcaatttat ttaaatagca tcgcaacatt ctataacaaa cagttattat
     1261 tctgaagtat gatgaaatca cgtattttta ttattatgat attttcaaag aatatcataa
     1321 taaaattttt aatagtctaa tatttacaag atcttctttc ggaagcataa atatttttta
     1381 cggtttgggc atatccaaat aaaaggttta tataggattc acaccgatgg ataaccttca
     1441 ggaagagaac attttagagt tttcagaaat ttcaaaatat tttccgggcg tcaaagctct
     1501 ggacaatgtt tcttttaccg cccgcagcgg tgaagtttta gcatttctgg gagaaaacgg
     1561 agccggaaaa tccactcttc ttaaaattct gaacggcgat taccaaccgg ataaagggaa
     1621 atatttgatt aatggcgttg aaaagaaatt caactctccc caggaagcaa tcgaagaggg
     1681 aatcagcgtc atttatcagg aacgtcagat tctgatggaa ctgaccgttg ccgaaaatat
     1741 ttttctggga agaatgccgg taaacaattt cggaattatt aatgtaaaaa aagcgaacaa
     1801 gatggctgcc gccattatta aggatttcgg gctgccaatc aatccgacca caaaagttaa
     1861 ggatttgagc atcgcctttc aacaaatggt cgaaattatg aaagcataca gccgggaaaa
     1921 tctcaaagtg atctgctttg atgagccgac ggcaagtctg agtgactcgg aaattgatat
     1981 cctcttcgga atcatccgaa aactgaagaa agaaaataaa atcattattt atgtttccca
     2041 tcgtctaaaa gaaattcaac agattactga tcgggttgcc atttttaaag acgggaaata
     2101 catcacgacc atcaagaccg gtgaagtgtc agaaaaagaa atgatcaaaa tgatggtcgg
     2161 cagagatctg ggtgatatct tcttaaatct tgatcgaaat aaagagatcg gagacgtatt
     2221 actcgacgtt aaggacgtct cttcggaatt tgtcaaagag tgctcttttc aactgcggaa
     2281 aggagaggtc ctcggcttca gtggtttggt aggtgccggc aggaccgaac tgatgcgtgc
     2341 cgtgatcggc gcggataaac gaaaaggcgg agaaatatct ttggaaggca aaaagattca
     2401 taactcttcg ccgaaggaag cgatgaaaaa cggtatcgta ctcgttccgg aagaccgaaa
     2461 aatgcagggg attctgtcaa atttaaatgt ttccggaaat attaatattt ctctgctcga
     2521 taagaatgca aataaattcg gaattattga cagcaaaaag gaactgcaaa ttgcggaaaa
     2581 atgtatcgat gaatttcaca tcaagacgcc atccccgcta aaaagaatag tcgaattgtc
     2641 cggcgggaat caacagaaat gcatcgtggc gcgcggaatt tccacgatgc ccaaagtgtt
     2701 gattttggat gagccgacca aagggattga tgtcggaaca aaatccgagt tttatcaaat
     2761 gatttgtgca tttgcaaaac aagggctcgg aatcattctc atttcttccg aattgcctga
     2821 agttttaggt ctttccgatc gaattattgt catgagatcc agaaaaattg tgggtgaaat
     2881 aaatcgggaa acagcaaccg aagcattggt tttaagtatg agcatgattg actaaatcgc
     2941 tgaaaaggat tgaaaatacc ggaaatgact atggaagaag aaaaaggaat caagaaaaca
     3001 atcactcgta taacaaactt tatcggcaac gataaattgg tattgctgat cgcgattatc
     3061 gtcgttttcg ctatgtttac ttttctaaat cccaatttta tgagcaagca gaacattatt
     3121 aacctgctgg tcgccggttc gctcatcgga ttggtagcaa tcgggcatac ctat
//
LOCUS       DAOMIV010000223         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_32635_length_3165_cov_4.063344, whole genome shotgun sequence.
ACCESSION   DAOMIV010000223 DAOMIV010000000
VERSION     DAOMIV010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32635_length_3165_cov_4.063344"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="PKV59_05985"
     CDS             complement(<1..538)
                     /locus_tag="PKV59_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46339.1"
                     /translation="MTIDSCNRIRKKIVKIVLPILFCTICSGCYLIETIQPTPLPQKT
                     PTSLININALPTLPTLPPAVETATPIPESDFTYDTTGIIFDHLTEEPVPTEPNQAARV
                     IGTVSEFSGSDLIIKNSSGDSYTITCDDFCFYVDNRKKIISGEAVRPGAEIAVFGATD
                     SEKPEKIHADAIILNGTVK"
     gene            complement(557..2464)
                     /locus_tag="PKV59_05990"
     CDS             complement(557..2464)
                     /locus_tag="PKV59_05990"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQP46340.1"
                     /translation="MNYGFIKAAACTPEIVVADCIHNAQAILDLMKKSAAEGVRLAVF
                     PELCLTGYTCGDLFTQQTLLESVKQNLLKLAEESRSLPMISVIGAPIAHYGKLYNCGV
                     VLYGGKILGIVPKTEIPNYAEFYEARNFCAAPETNETVRIGEDEIPFGKRLLFCCNEM
                     PEFIFGIEICEDLWVCRPPSTGHAIAGATVILNLSASNEIVGKADFRRQLVESQSARL
                     VCGYLYADAGEGESTTDLVFSGHNLIAQNGHILAEAQPFSSGYCVTELDLEELMSERQ
                     RLTTFPRQDATGYQMIPFNMEKTPVQLTRNISKNPFVPADSADLRDRCEKILAIQSAG
                     LRKRINHTHVKTVVIGVSGGLDSTLALLVSVRAMKESGHLPSDVIAVTMPGFGTTRRT
                     KNNAIKLCESLGVTLRKIDITRSVRSHFRDLGQDENDHNVLFENAQARERTQILMDIC
                     HQTDGFVVGTGDLSELALGWATYNGDHMSMYGVNASVPKTLVRYLVRYYADYLADKQV
                     SEILSDILNTPVSPELLPAAEGKISQLTENIIGPYELHDFFLYYFIRHGFSPEKVACL
                     ANYAFKDTYSADEIQKWLKVFIKRFFNNQFKRSCLPDGPKVGSVSLSPRGDWRMPSDA
                     SFKIWIDSLDR"
     gene            complement(2701..>3165)
                     /locus_tag="PKV59_05995"
     CDS             complement(2701..>3165)
                     /locus_tag="PKV59_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP46341.1"
                     /translation="FTDTVMENIRYGNRNATDEQVIHAAKLANADFFITHLPNGYQTV
                     LYDDGENLSQGQRQLLAIARAAVKDPPVLILDEATSSIDTRTEALIEKGMDQLMEGRT
                     VFVIAHRLSTVRNSNAILVLEHGEIIERGDHEDLLAQKGRYYQLYTGMFELS"
BASE COUNT          825 a          739 c          728 g          873 t
ORIGIN      
        1 attttaccgt cccattcagt ataatcgcat ccgcgtggat tttttccggt ttttcggaat
       61 cggtcgcacc gaaaacggcg atctcagctc ccggccgaac ggcttctccc gagatgattt
      121 ttttccgatt atccacataa aagcaaaaat catcgcaggt tatcgtgtaa ctgtctcccg
      181 atgaattctt aatgatcaga tccgaaccgg aaaattccga tacggttccg atcactctgg
      241 ctgcctgatt cggttccgta ggaaccggtt cttccgttaa atgatcaaaa ataataccgg
      301 ttgtgtcata cgtaaagtcg gattccggaa tgggcgtggc cgtctcaacc gccggcggaa
      361 gggtcggcaa agtcggaagc gcattgatat tgatgaggga agtgggtgtt ttttgcggca
      421 acggtgtcgg ttgtatcgtc tcaatcaaat aacaaccgga acagatagtg caaaataaga
      481 ttggcaacac aatttttacg attttttttc gaattcgatt acacgagtcg atggtcatga
      541 atgaaaaatc tccggtttat cggtcaagcg aatcgatcca tattttaaaa gaggcatcgc
      601 ttggcatgcg ccaatcccct cgcggcgaaa gcgaaaccga accgactttc ggtccgtcgg
      661 gaagacagga tcgtttgaac tgattattga aaaaccgttt gataaaaact ttgagccatt
      721 tttgaatttc gtcagcgcta taagtatctt tgaatgcata atttgccaga caagcgactt
      781 tttcggggga gaaaccgtgc cggatgaaat agtagagaaa gaaatcatgt aattcatacg
      841 ggccgatgat attttcggtc aattgcgaaa ttttcccttc cgcggcaggt aataattcgg
      901 ggctgacggg tgtattcaaa atatccgaaa gaatctcgga aacttgtttg tccgccagat
      961 aatccgcata atagcgtacc aaatatctta ccaatgtttt cggtacggag gcgttcaccc
     1021 catacatact catgtgatcg ccgttgtatg tcgcccagcc cagggctaat tccgaaagat
     1081 ctccggtacc gaccacaaat ccgtccgttt gatggcaaat atccatcagg atctgagttc
     1141 gttctcgggc ttgagcattt tcaaagagga cattatgatc attttcgtct tgtcccaagt
     1201 cgcggaaatg actgcgaaca ctgcgggtaa tgtcaatttt tcgcaaggtt accccaagtg
     1261 attcgcatag tttaatggcg ttattcttgg ttctgcgagt tgtcccaaaa ccgggcatcg
     1321 taaccgcaat tacatcggag ggaagatgtc ccgattcctt catggctcga acactgacca
     1381 acaaggcaag cgttgaatcc aatccgccgg agactccgat caccactgtt ttaacatgag
     1441 tatgattgat ccgtttccgc aatccggcgc tttgaatagc gagaattttt tcgcagcgat
     1501 cccgtaaatc ggcggaatcg gcgggaacga aaggattctt ggaaatattg cgtgttaact
     1561 ggacgggggt tttctccata ttgaagggaa tcatttgata accggtcgcg tcttgtcgag
     1621 gaaatgtggt taaccgttgc ctttcagaca tcaattcttc caaatccagt tcggtaacgc
     1681 agtatccgct agaaaaaggt tgtgcttccg ccaaaatgtg accattttga gcgatcagat
     1741 tgtgtccgga aaaaaccaga tcggttgtcg actctccttc acccgcatcc gcatagagat
     1801 aaccgcagac caggcgggcg gattgacttt cgacaagctg ccggcgaaag tcagccttcc
     1861 cgacgatttc gttactggca gataagttca ggatgacggt ggcgccggct atggcgtgac
     1921 cggttgacgg cggccggcaa acccaaaggt cttcgcagat ctcaatcccg aaaataaatt
     1981 cgggcatttc gttacaacag aagagcaatc tttttccaaa tggaatttca tcctctccga
     2041 tgcggactgt ctcgttcgtt tcgggagcgg cacagaagtt tcgcgcttca taaaattcgg
     2101 cataattggg aatttccgtt ttggggacga ttcccaaaat ttttccgccg tacaggacca
     2161 caccgcagtt atataacttc ccgtaatgag cgatcggcgc tccgataacg gaaatcatcg
     2221 gaagggagcg gctttcctcg gctaatttca gcagattttg ttttacggat tctaacaatg
     2281 tttgttgggt aaaaaggtca ccgcaggtat aaccggtcag gcacaattcc gggaagacag
     2341 ccaatcggac tccttctgcc gcgctttttt tcatcagatc caggatagct tgggcattat
     2401 gaatgcaatc ggcaacaacg atttccggcg tacaggccgc tgcttttatg aatccgtaat
     2461 tcatgcatat cctccataca gaacagaaat cgatctgtca tgatcgtccg attttcattt
     2521 tgattctatc aaccattcag aatgattccg aatagaatct tttaaatcgt gtgtttaacc
     2581 gttattctgt attaatcata acactccctt tttccctcgt tttgtctgga tttgattgaa
     2641 taaaatcaca gttcaaaaaa ggaaacatcg ggagtgtgtt gaattcacat gatggcctaa
     2701 ttaagaaagc tcgaacatcc ccgtataaag ttgataatag cgaccttttt gtgccagcag
     2761 atcttcatgg tcaccgcgtt cgataatttc tccgtgttcc aaaaccaaga tggcattgga
     2821 attccttacc gtcgaaaggc ggtgggcaat cacaaaaacc gttcgtcctt ccattaattg
     2881 atccatacct ttttcgatca aagcttcggt tctggtatcg atggaactgg tcgcttcgtc
     2941 taaaatcaac accggcggat cctttacggc tgcgcgtgcg attgccagta attgccgctg
     3001 tccctggctc agattttcgc cgtcatcgta caacaccgtc tgataaccgt ttggaaggtg
     3061 agtgataaag aaatcggcgt tggcaagttt cgccgcatga atcacctgtt catccgtcgc
     3121 attccgattc ccgtaacgga tattttccat aacggtatcg gtaaa
//
LOCUS       DAOMIV010000224         3165 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_32640_length_3165_cov_3.238264, whole genome shotgun sequence.
ACCESSION   DAOMIV010000224 DAOMIV010000000
VERSION     DAOMIV010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32640_length_3165_cov_3.238264"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /locus_tag="PKV59_06000"
     CDS             <1..1639
                     /locus_tag="PKV59_06000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQP46342.1"
                     /translation="NSKRDQSSGEENFELWSSVLKFDIYSAKEEVILSEVSEEGREIE
                     ISPDGRWLAYFYVQKAESPYEEPYYVAVNSCVEKAENKIISTEDQTVINFRWDSDSKH
                     IYFLVHDHGDGKIVQNDLAGGTPREIVYGKRMVEQFDVSTDGKMIAFSVSSPITPGDL
                     FIKNVDTGEENQLTHFNKDFETSHAFSIPDELEFTGTDGLSIQGWYFKPKDFNPQKTY
                     PAVVEIHGGPQIMWGNSFWHEFQVLCSRGYFVFFCNPRGSAGYGARFQKLRGKGGYTD
                     MADIMNGLDAFLAKERSVDQNRLCVTGGSYGGFLTAWIVTHTGRFAAAVAQRGVYDEL
                     NMFGSGDIPESVEWYYGGIPREENLKELWEYSPIAHAKNVTTPLLILYSELDYRCPIS
                     QAESFFTHLRRNGNRGVNMVRFPREGHNLSRNGEPWHRIERLRRITEWFDHQIKYTSL
                     IDEILTDDQLNQWIYTLKGWRWENLKLIRSTESGNNDIANMMVRKIWNYLKMNRKQCN
                     LIIEGSRLTVEIQGIDSRGITMREILLADRLNKIFFA"
     gene            complement(1754..2893)
                     /locus_tag="PKV59_06005"
     CDS             complement(1754..2893)
                     /locus_tag="PKV59_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQP46343.1"
                     /translation="MKLTTDWYIKKMSQIQSKLSEEKLDAILLLDPYNIFYASGFFHQ
                     STERPLGLLIPQMGEPSLYVPKLEQEMASETWIKSIYVYFDFPGLIHPIAWMLKENSQ
                     YSHIGVDQLNYRQWKIAQEVCPNIQISDLVYTMRLVKDAEEIQILECASVYADYMVEM
                     TKDAIIEGLSELDAYNYARDHTVNKMIKELGELTFANQGTTNGAVLYGAHSAHPHGLM
                     SSTLKPKPGDVIEAGFGALVCTYESESEHTFIYGEPDHQTVSYFNAMYNAWKAGLESA
                     KPGVKCCDVNKAALEEIRRAGYEEFLRHRMGHGKGLQEHEPPWVAEGDMTVLKPGMII
                     SDEPGLYVPGFGGFRHSDTLLITEDGCRRLTKYPRELEACIIPIK"
     gene            complement(2907..>3165)
                     /locus_tag="PKV59_06010"
     CDS             complement(2907..>3165)
                     /locus_tag="PKV59_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQP46344.1"
                     /translation="TALTEMGFSCIPPRAGFYIFTDVTSLGMTAEEFCMQLLMQAKVL
                     IFPGSLFLDPTGRYVRISLLSPTERIIAAAERMKNFVKQYK"
BASE COUNT          985 a          588 c          691 g          901 t
ORIGIN      
        1 aaattcaaaa agagaccaat catcgggcga ggagaatttt gaattatggt cttcagttct
       61 aaaatttgat atttactcag caaaagaaga ggttatttta tctgaagtct cggaggaggg
      121 aagagagata gaaatttcac ctgacggaag gtggcttgct tatttctatg tccaaaaagc
      181 agaaagtccg tatgaagaac cttactatgt tgccgtaaac tcatgtgtgg aaaaagctga
      241 aaataagatt atcagtacgg aagatcaaac cgttatcaat tttaggtggg atagtgacag
      301 caaacatata tattttttag ttcacgatca tggtgacgga aaaattgttc aaaacgattt
      361 agcagggggg acgccgagag aaattgttta cggaaagagg atggtcgagc aatttgatgt
      421 cagtacggac ggtaaaatga ttgcattttc cgtatcgtcg ccgattacgc ccggtgattt
      481 atttataaaa aatgtcgata cgggagaaga aaatcaactg acacatttca ataaagattt
      541 tgaaacttct catgcctttt caattcctga tgaactggag tttaccggta cagacggtct
      601 gagtattcaa ggatggtatt tcaaaccgaa agattttaat ccgcaaaaaa cctaccctgc
      661 tgttgtcgaa attcatggag gaccgcaaat tatgtgggga aactcttttt ggcatgaatt
      721 tcaagtattg tgttcaagag gatattttgt cttcttctgt aatccgagag gttcagccgg
      781 atatggtgcc cgatttcaaa aattaagagg taagggcgga tataccgaca tggctgatat
      841 tatgaacggt ttggatgcat ttcttgccaa agaaaggtcg gttgatcaaa acagattatg
      901 tgtaacggga gggtcatacg gcggattttt gaccgcctgg atcgtcacac ataccggcag
      961 gtttgctgcc gctgtggcac aaagaggagt ctatgatgaa ttaaatatgt ttggcagcgg
     1021 tgatatcccc gaatcggtag aatggtacta cggcgggatt ccgcgggagg aaaatttaaa
     1081 agaactttgg gaatattctc cgatcgcgca tgcaaaaaat gtgaccacac cgttgttgat
     1141 attatatagc gaattagact atcgatgccc aatcagccag gcagagagct tttttactca
     1201 tttaaggaga aacggaaacc gtggtgttaa tatggttcga tttccacgcg aagggcataa
     1261 tctttcaaga aacggagaac cttggcatcg aatcgaacga ttaagaagga ttaccgaatg
     1321 gtttgatcat cagataaaat acaccagttt aatcgacgaa atattaactg acgatcagtt
     1381 aaaccaatgg atatacactc tgaaaggatg gcggtgggag aatttaaaat tgatacgatc
     1441 aactgaaagc gggaacaacg atatcgccaa tatgatggtt cgaaaaattt ggaactattt
     1501 gaaaatgaat agaaaacaat gtaatttgat tatcgaagga agcaggttga ccgttgaaat
     1561 acagggaatc gattcccgtg gaattacaat gagggaaatt ttgcttgctg atagactcaa
     1621 taagattttt ttcgcttaaa agttgaaagc acaaaaaaac gatcagaaat tgttgaagat
     1681 tataaactaa accggagatg ggcaattgcc catctccggt ttagtttata acaaaaagga
     1741 gagaggatat atcctattta atcggtataa tgcatgcttc taattcgcga ggatatttcg
     1801 tcaggcggcg acagccgtct tcggtaatca gcaatgtatc ggaatgccga aatccgccaa
     1861 agccggggac atatagcccg ggttcgtcac tgatgatcat tccgggttta agaacggtca
     1921 tgtcaccttc ggcaacccat ggcggttcgt gttcctgtaa tccttttccg tgacccattc
     1981 ggtgacgaag aaattcttca tatcccgctc ttctaatttc ttctaatgcc gctttgttta
     2041 catcacagca tttcacaccc ggttttgcag actccaatcc ggctttccaa gcattgtaca
     2101 tggcattaaa ataagaaacc gtctgatgat ccggttctcc ataaatgaag gtatgttcgc
     2161 tttccgattc gtaggtacaa accaatgcac cgaaaccagc ttcgattaca tctcccggtt
     2221 tcggtttcaa agttgatgac atcagaccat gcggatgagc agaatgagct ccgtagagaa
     2281 ccgcgccgtt ggtggttcct tgattcgcaa atgtcaactc acccaattcc ttgatcattt
     2341 tatttacggt atgatcgcgt gcataattat aagcatctaa ttcggataac ccttcaataa
     2401 ttgcatcttt tgtcatttca accatataat cggcataaac agaagcgcat tccagaattt
     2461 gaatttcctc ggcatctttt accagtcgca ttgtatatac caaatcgctg atttgaatat
     2521 tcggacatac ttcctgtgcg attttccatt ggcgataatt taattgatcg acgcctatat
     2581 ggctatattg tgaattctcc ttaagcatcc aggcaatcgg atggattaaa ccggggaaat
     2641 caaaataaac ataaatggat ttaatccagg tttccgatgc catttcctgt tccagtttgg
     2701 gaacatataa agacggttcc cccatttgag ggatcagcag acctaaaggc ctttcggtag
     2761 attgatggaa gaatcctgaa gcgtaaaaaa tattatatgg atcaagaaga agaatagcat
     2821 ccaatttttc ttccgataat ttagattgga tttgggacat ttttttaatg taccaatcag
     2881 ttgtcaattt catgatttca ccgaagttat ttatattgtt taacgaaatt tttcattctt
     2941 tccgcagcag cgataatacg ttctgttgga gaaagaagag aaattcttac ataacgaccg
     3001 gtaggatcaa ggaacagtga ccctggaaaa ataagcactt ttgcttgcat taacagctgc
     3061 atacaaaatt cttctgcggt catacccaaa cttgtcacat cagtgaaaat gtaaaatcct
     3121 gcgcggggag gaatacaaga aaaacccatt tcggtaagtg ccgtt
//
LOCUS       DAOMIV010000225         3164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_32650_length_3164_cov_3.874879, whole genome shotgun sequence.
ACCESSION   DAOMIV010000225 DAOMIV010000000
VERSION     DAOMIV010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32650_length_3164_cov_3.874879"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /gene="aspS"
                     /locus_tag="PKV59_06015"
     CDS             <1..1273
                     /gene="aspS"
                     /locus_tag="PKV59_06015"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQP46345.1"
                     /translation="PEGARDYLVPSRIYPGEFYALPQSPQQLKQLLQVAGVERYFQIA
                     RCFRDEDQRGDRQPEFTQLDLEMSFVERDDILDLMEDLFTKMCEDLVPQKKILFSPWP
                     KLTYQEAMDRFGKDSPDLRFGMELVNISDLAKDCGFTVFESAIKDGGSVRGINAKTLG
                     EYTRKQLDELTDQVKLWGGKGLAYVKISESGEESSPFKKFFSNEKWEELLKRMEAEKG
                     DLLLFVADSKPAVVYDCLARLRVMLGDRLGLRDNNKLAFCWIVDFPLFEWSEEEQRWD
                     ATHHPFTRPKKEDFDLLSTDPGKVRGDQYDMVLNNYELASGSIRIHERWLQEKVFDLI
                     GLPRDVARDRFHQIMDAFEYGAPPHGGIAPGIDRFVMLMADEPNIREVIAFPKNNAAR
                     DIMSGAPSPAMEGQLRELHIKVVYPEEEKKS"
     gene            1472..2395
                     /locus_tag="PKV59_06020"
     CDS             1472..2395
                     /locus_tag="PKV59_06020"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQP46346.1"
                     /translation="MIMEIVFWQMLTLFIIIVVGLICGRTRVITESETKGISGLIINV
                     FNPALVFSSVINNRGRVQDNMLQSITIIAIAMFAGLILIGIVTAGFFSKDKDQQIMYK
                     LMLAFPNVGYIGIPLINSIYGPSASIYVAIFILLFNIVIYTYGIALLKGKTGKRLSVE
                     NLKSILNMGTISCLITLLFFIFGIPVPDIVKTPITYLGNCATPLALMSVGFSLANAKV
                     SDVFMDKRLYIFSAFKLLLLPAIGVFLLKLFPLPEQVIGISAIMFAMPTGNLPVILAN
                     RYGVNSDICSSAIIITTLLSVLTIPLIAGMI"
     gene            2430..3104
                     /locus_tag="PKV59_06025"
     CDS             2430..3104
                     /locus_tag="PKV59_06025"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HQP46347.1"
                     /translation="MLIGVIVNAAAVAAGGILGNVLHKGIPEKMNQLMMQGLSLCVLY
                     VGISGSLEGNNTIVVVLSVVFGAVIGELFNLDGKLQSFGDFLQSKVKSDNPISEGFIT
                     CSLFVCVGAMAIVGSLQSGLTGNNETLFTKGLIDGIIAMIMASTMGIGVAFAAVPLFI
                     YQGILTVSAGAISGYLTMNVINEITCTGSLLIIAIAFNMLKLTNIKTANLLLAPFLPI
                     LFCRFF"
BASE COUNT          813 a          619 c          768 g          964 t
ORIGIN      
        1 acccgaaggg gctcgggatt atctcgttcc ctcccggatt taccccggag aattctatgc
       61 tctgccgcaa agtccacaac aattgaagca gttgcttcag gttgctggtg tggagcggta
      121 ttttcagatt gcgcgatgtt tccgcgatga agatcaacgt ggtgaccgcc aaccagaatt
      181 tacccagttg gatttggaaa tgtcatttgt cgagcgcgat gatattctgg atttgatgga
      241 agatttattc acgaagatgt gcgaagattt ggttcctcag aagaagatcc tgttcagccc
      301 ctggccaaaa ttaacttatc aggaagcaat ggatcgtttc gggaaagatt cacccgattt
      361 acgcttcgga atggaattgg tgaatatcag tgatttggcg aaggattgcg gctttaccgt
      421 ttttgaaagt gctatcaaag acggcggcag tgtccgcggg atcaacgcaa aaacgttggg
      481 agaatacacc cgaaaacaac tggacgaatt gaccgaccag gtaaaactct ggggcggcaa
      541 agggttagcc tatgtaaaaa tcagtgagtc gggagaagaa agttctccgt ttaaaaaatt
      601 tttctcgaac gagaaatggg aagaattgtt gaaacgaatg gaagccgaga aaggcgattt
      661 gctcctgttt gtggctgaca gcaagccggc tgtggtgtat gactgtctgg cacgattacg
      721 agtcatgctg ggagatcgcc tgggacttcg tgacaataac aaattagctt tttgctggat
      781 tgttgacttt ccgttgtttg aatggtcgga agaagaacaa cgatgggatg cgacccatca
      841 ccccttcact cgtcccaaga aagaagattt tgatttactc agtactgacc ccgggaaagt
      901 tcgtggagat cagtatgata tggttttgaa taactatgaa ttggcatccg gatccatcag
      961 aatccatgaa cggtggctgc aggaaaaagt atttgatctg atcggattac cgcgtgatgt
     1021 ggctcgcgat cgattccacc aaattatgga tgcgtttgaa tatggagcgc cgccgcacgg
     1081 aggaatcgct cccggaatcg atcgttttgt catgctgatg gcggatgaac cgaacattcg
     1141 cgaagtgatc gcattcccga agaacaatgc cgctcgcgat ataatgagcg gtgcgccttc
     1201 acctgccatg gaaggacaat tacgggaatt acatattaaa gtcgtctatc cggaagaaga
     1261 aaagaaatcc tgatttttct tttataagat catcggggct gtctgaaaac tcgggcagcc
     1321 ccttttcgtt tacgacggga gaaaaaagcg gttggaatca ttctttttcc ccgattcttc
     1381 gctgaatttc tgggatttgc ttgaaacggg attattattc ttgcggcagg ggagatcccc
     1441 ggaatcaagc ggtaaaggag ttttatttct catgatcatg gaaattgtct tttggcaaat
     1501 gttaacgtta ttcataatca tcgtagtggg tttgatatgt ggccgcactc gagttattac
     1561 cgaaagcgaa acaaaaggca tttccgggct gatcattaat gtcttcaatc cggcattggt
     1621 tttttcaagc gtgattaaca atcggggaag agttcaggat aatatgctcc aatcgattac
     1681 gattattgca attgccatgt ttgccgggtt gatcctaatc ggaattgtga cagccggttt
     1741 tttttcaaaa gataaagatc agcaaatcat gtataaactg atgcttgctt ttccgaatgt
     1801 cggttatatc ggtatcccgc ttatcaattc catttacggc ccttccgctt ccatttatgt
     1861 ggcgatcttt attctgctgt tcaatattgt catatatacc tatggtatcg ctttgttgaa
     1921 aggcaaaacg ggaaagagac tttctgtcga gaatctgaaa agcattctca atatgggcac
     1981 aatttcctgt ctgattaccc ttttgttttt tatttttggg attccggtac cggatatcgt
     2041 gaaaacgcca attacttatt tggggaattg tgccactccg ttggcattga tgtccgtcgg
     2101 tttctctctt gccaacgcaa aagtttccga tgtttttatg gacaaaagac tttacatttt
     2161 ttctgccttt aaattattgc ttttgccggc aatcggggtt tttctgttga aactgttccc
     2221 gctgcctgag caggttatcg ggatcagcgc aatcatgttt gccatgccca ccggaaatct
     2281 tccggtaatt cttgccaatc gatacggtgt aaatagtgat atttgctcga gcgctattat
     2341 tatcacaacg ctcctgtcgg ttttgacgat cccgctgatc gcgggaatga tctgaagcat
     2401 tttctgttct atcgaggttg aatctgtcta tgttaatcgg agttatcgta aatgcggcag
     2461 ccgttgcagc cggcggaata ttgggaaatg ttcttcataa aggtattccc gagaaaatga
     2521 atcagttgat gatgcaaggc ttaagccttt gtgtactgta tgtgggaatc agcggatcat
     2581 tggaaggaaa taacacgatt gtcgtggtat tgtccgttgt gttcggagcc gttatcggtg
     2641 agttattcaa tctcgacggg aaacttcaat cctttgggga ttttttgcaa tccaaagtaa
     2701 aaagtgataa tcccatttca gaaggattta ttacctgcag tctttttgtc tgcgttggtg
     2761 ccatggcaat tgtcggttca ttacaaagcg gtttaaccgg aaataacgaa acactattta
     2821 ccaaagggtt gatcgacggt attattgcga tgattatggc ttccacgatg gggattggcg
     2881 ttgcttttgc cgccgttccg ttatttattt atcaaggtat attgactgtg tcggcgggtg
     2941 cgatttcggg atatctgacg atgaatgtga ttaatgaaat cacctgtacg gggtcgttat
     3001 tgattatagc cattgcattc aatatgctga aactcacgaa tataaaaacg gctaatttgt
     3061 tgcttgctcc gtttttgccg attctgtttt gcagattttt ttgattctaa ggtaaaaatc
     3121 actttccaag accttccgat ttacaacgtt tttccgatat gata
//
LOCUS       DAOMIV010000226         3145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_32948_length_3145_cov_4.247249, whole genome shotgun sequence.
ACCESSION   DAOMIV010000226 DAOMIV010000000
VERSION     DAOMIV010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32948_length_3145_cov_4.247249"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..3128)
                     /locus_tag="PKV59_06030"
     CDS             complement(282..3128)
                     /locus_tag="PKV59_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQP46348.1"
                     /translation="MLYSREIDFTLNGKEISCEAEPTDTLLDLLRYRGCYSVKRACYT
                     GECGNCAVLMDGRLLPSCVMPAPQAQGHTILTVEGLNEGKDLNPIQKAFVETGAIQCG
                     FCTPGMVLATKALLDKNLYPSLDDAKDALGSILCRCTGYYQPLDAVMRAAAYLRGETV
                     PPYTKADESVLFTNESCPDFSAIRQSVQKVDAVKMVKGKPVYTDDIELPGMLHGMLMT
                     SPYAHARIKNIDTSAAEALPGVHAVLTYKNVKRYVYGSGGQTYPNPKPWDQVCLDNKV
                     RFVGDRVAAVAAESVELCKKAISLIKVDWEILPAVFDMEEAMKDGAPIIHDEPDCIDV
                     ADASHNKAYVIDANYGDLEKDFAESDLVVERVFHTQQVQQTPLEPHIVISWLDENDRL
                     VIRTSTQVPFHVRRIISPLIDVPVQNIRVIKPRIGGGFGAKQEVLLEDLCALLTLRTG
                     RPVRFYFDRQDEFRSSRSRHPDTIRFKVGVTKSGRIKAIEMDMIENTGAYGVHGFTVC
                     NVTGTRGLSTYRADSMRFHADIVYTNLPVPGAFRGYGGPQGIYGIECIIDEVAEKLKM
                     DPIKFRMKNVVRPGEKIKIMDKLAEFDPVPQKVNSYGLDACIRKGMEAIDWNRRKSKQ
                     FHSDPSKPNICRGIGMSIMMHGTAIAGIDMGAAAMKINDDGSFNLNVGATDLGTGSDT
                     ILAQMAAEVLKIPVSKIVVYSSDTDFTPFDVGAYASSTTYISGMAVKKCAEKIKEQVL
                     ERAALMLQQPKDDLYLKDEKVYAPNGDHVTMEEIALHSLHRKDQRQIAAYASHVSPEC
                     PPVFGTEFAEVEVDIETGQVTPIKLVMTVSPGTVINPTTAIGQLEGGQTTALGYALCE
                     EMCYDNQGNLLNPRFGDYHILQADEMPLMESYIIQEGDGEKTGPFGAKALAEIPTDGV
                     APAIANAVANAIGSRIDSLPIYPEKVWRAIKSQH"
BASE COUNT          816 a          791 c          682 g          856 t
ORIGIN      
        1 tataacttta cgcgggcagc cgaaacggta aacgacgaac aaattttgat catcgataat
       61 cgcaaaatag ccgatcaatt tgaagaagaa ttcgaaaaaa ttttagaaaa ggcaaattaa
      121 tcaatttttg aagaatattt gcggatcatt ccgataatat ttgtgaaaat acttattaaa
      181 aagaagttgt cctctgataa aaggacagct tctttttgaa tcattctctg agctttatag
      241 agcaaacgtt gaactcgtca gcagtgtgtt ttccgataag attaatgctg tgactttatt
      301 gcccgccaaa ctttttccgg gtaaattgga agcgaatcaa tccgagaccc gatggcgttc
      361 gctactgcat tggcaattgc cggagcgact ccgtccgtcg gtatttcagc taacgctttt
      421 gcgccgaaag gaccggtctt ttcaccgtcc ccttcctgaa tgatatagct ttccatcaac
      481 ggcatttcat ccgcttgcag aatatggtaa tcaccgaatc gcgggttcaa caaattccct
      541 tggttgtcat aacacatttc ttcacaaagg gcataaccga gcgcggttgt ttgcccgcct
      601 tccagctgtc cgatagcggt cgtcgggtta atcaccgtcc ccgggctgac tgtcataacc
      661 aatttaatgg gagtcacctg gccggtttcg atatccactt ccacttcggc gaattcggtt
      721 ccgaagaccg gcggacactc cggagaaaca tgggaagcat aagccgcgat ttgtcgctga
      781 tccttacgat gaaggctatg caatgcaatc tcctccatag taacgtgatc cccattcgga
      841 gcgtaaacct tttcatcttt cagatacaga tcgtctttcg gctgctgcag catcaaggcg
      901 gctctttcca gaacctgttc tttaattttt tcggcacatt ttttgactgc catcccggaa
      961 atataggtgg tactggatgc ataagcgcct acatcaaacg gcgtaaagtc cgtgtccgaa
     1021 gagtagacaa cgattttgga gacgggaatt ttcagcactt ccgctgccat ttgtgccaat
     1081 atcgtatccg aaccggttcc caggtcggtc gctccgacgt tcaaattgaa agaaccgtcg
     1141 tcattaatct tcatcgctgc ggcacccata tcaattccgg caatggcggt cccatgcatc
     1201 ataatggaca tcccgatccc tctgcagata ttcggcttcg agggatccga atgaaactgt
     1261 ttggatttcc tgcgattcca atcgatcgct tccatccctt tccgaataca ggcatccaat
     1321 ccgtagctgt taactttttg cgggaccgga tcgaattctg ccagtttatc cataattttg
     1381 attttttcac cgggacgaac aacatttttc atccggaatt taatcggatc catttttaat
     1441 ttttcggcga cttcgtcgat aatgcattcg atcccgtaaa ttccttgcgg tccgccataa
     1501 ccgcgaaagg ctccggggac cggtaaattg gtataaacaa tatccgcatg gaatcgcatg
     1561 ctatctgccc gatatgtgga taatccgcgt gttccggtca cattacagac agtaaaacca
     1621 tgcacgccgt aagccccggt attttcgatc atatccattt caatcgcttt aatgcgcccg
     1681 gatttcgtga cgccgacctt aaaacgaatg gtatcgggat gacgcgaacg agaggagcgg
     1741 aattcatctt ggcgatcgaa ataaaatcgg acgggccttc cggttcgcag cgttaacaac
     1801 gcacataagt cttcaagtaa aacttcttgt tttgcgccga atcctccgcc gatccgcggt
     1861 ttaatgacac ggatattttg aaccggaaca tcgatcaaag gagaaataat tctccgaaca
     1921 tggaaaggaa cctgtgtgct ggtacggatt accaaacgat cgttttcatc cagccagctg
     1981 atgacgatat gcggttccaa aggagtttgc tgaacttgct gtgtatggaa tactctttcg
     2041 acgaccaaat cggattcggc aaaatccttc tctaaatccc cgtaattcgc atcgatcaca
     2101 tacgctttat tatggctcgc atccgcgaca tcgatgcaat ccggttcgtc atggataatc
     2161 ggagcgccgt ctttcatcgc ctcttccata tcaaataccg caggcaggat ttcccaatcc
     2221 actttgatga ggctgatggc tttcttgcat aattccaccg attcggcagc cacagccgcg
     2281 acccgatcac cgacaaagcg cactttattg tcgagacaaa cctgatccca tggtttcggg
     2341 ttcggataag tttgaccgcc gctaccgtaa acatagcgtt tcacattctt ataggttaat
     2401 accgcatgaa cgcccggcag cgcttccgcg gcggaagtat cgatattttt gatacgggcg
     2461 tgagcatagg gactggtcat taacattccg tgcaacatcc ccgggagttc gatatcatcg
     2521 gtataaaccg gcttgccctt aaccatcttg accgcatcaa ctttttgtac tgattgccga
     2581 atggcggaaa aatcgggaca ggattcgttg gtaaataaaa ccgattcgtc ggctttggtg
     2641 tacggcggaa ccgtttcacc gcgcaaatat gcggcagccc gcatcacagc atccaacggc
     2701 tgataataac ctgtgcaacg acataagatg gatccaagtg cgtccttggc atcatccaag
     2761 gatggatata aattcttatc cagcagtgct ttcgttgcca aaaccattcc cggcgtacag
     2821 aaaccgcatt ggatcgcacc ggtttccaca aacgcttttt ggatcggatt caaatctttc
     2881 ccttcattca atccttcaac ggtaaggatt gtatgaccct gagcctgagg cgcaggcatt
     2941 acgcaagatg gtaataatct tccatccatt aaaacggcac agttcccgca ttctccggta
     3001 taacaagctc tttttacact atagcaaccc cggtatctta agaggtctaa taaggtatcg
     3061 gtcggctcgg cttcgcagga gatttctttc ccatttaatg taaaatctat ttctcggcta
     3121 tataaaatag gtcaaagcag ttgta
//
LOCUS       DAOMIV010000227         3140 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_33036_length_3140_cov_4.058995, whole genome shotgun sequence.
ACCESSION   DAOMIV010000227 DAOMIV010000000
VERSION     DAOMIV010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33036_length_3140_cov_4.058995"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="PKV59_06035"
     CDS             complement(<1..1452)
                     /locus_tag="PKV59_06035"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HQP46349.1"
                     /translation="MNDFFGKSKAGARLFVFVFLATLIFFIGIFAVQADYRFSIPEDL
                     VIFWVEKDGTVSVSYEFTIQNYGQDIDYIDIGMPNNNFSLRDVIAKIDDQTVDQDKIS
                     YVDPDEVGYSYGITIDNTENPIPRNAKASVYVEVQNIKNVLYLSSDSSDGTEYVSFRF
                     MPNYFGSDFTTGTTKLTVRLVLPPGMSSSDPSYFTPENWVGDDEPDSWITAEGNIVYE
                     WQSDHADSSSPYTFGAKFPKEFLNPDVKIQTESQVKNNDSGGIGSLIFIFVVGLLIII
                     PLIRSFSSSKKKKNNIVPGSYLPPSIKAEGEGIKRGLTAVEAAVLLEKRIDTIISMII
                     FSLVKKNAIQVIQQNPLKIKVSEPLPEDLYDYEKGFIEAMTSEKDSEKKAKMEKTISN
                     LINSVAEKMKGYSLEETKAYYRSIIEKAWDQVKEAGTPELKGEKLDEIFGWAILDEDV
                     DKKSQEVFGDYPVFLPTWWWRVGPVYASPTPGHP"
     gene            complement(1471..1815)
                     /locus_tag="PKV59_06040"
     CDS             complement(1471..1815)
                     /locus_tag="PKV59_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46350.1"
                     /translation="MKIIGYIISGICIFFGVLFIWSAFGTPFSSSDLIIGLITTAIGI
                     VILYVTTKNNRKTTNIYKVDLPGEIKMNPKSCQKCGGPLNADNFTIVNGAPTVKCPYC
                     GAVYEVTEEPKW"
     gene            2069..>3140
                     /gene="ligA"
                     /locus_tag="PKV59_06045"
     CDS             2069..>3140
                     /gene="ligA"
                     /locus_tag="PKV59_06045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQP46351.1"
                     /translation="MEESEDLRKKYLQLKQEINYHNYRYHVLDDPEISDEAFDKLLVE
                     LRKIEEMHPEWITPDSPTQRAGSAVSEKFEKVTHPVQVLSLANAFNKQDVINWLDRTE
                     KMDPRIKYADFVLEPKIDGLTVVLTYQNGLLIRGATRGDGVIGEDVTPNIRTIKSVPL
                     AIPVSEKSVKVPETIVVRGEVFILESDFEKINAELAKRGEKTWVNPRNTAAGSLRQLN
                     PEITAQRPLRIFTYQILFSSADQPIPDTQWGTLEYLQQLGFPVSPDSRYCENREQMLN
                     HLDEWQEIRNRLDYEIDGVVIKINDLRIAGSLGFVGKDPRGAIALKFPAREATTKLLD
                     IGVNVGRTGVITPYAILSPVEIG"
BASE COUNT          921 a          659 c          645 g          915 t
ORIGIN      
        1 gggatgaccg ggcgtgggag aagcgtatac cggtccgaca cgccaccacc atgttggtag
       61 gaaaaccgga taatccccga atacctcttg cgattttttg tcaacatcct cgtcaaggat
      121 tgcccagcca aagatttcgt ccaatttttc accttttaat tcgggcgttc cggcttcttt
      181 tacttggtcc caagcttttt cgataataga ccgatagtaa gctttagtct cttccaaact
      241 atagcctttc attttttcag ccacggaatt aatcaagttc gaaattgttt tttccatctt
      301 cgcttttttc tcagaatctt tttcgctggt catcgcctcg ataaaaccct tttcataatc
      361 gtaaagatct tcgggcaacg gttccgaaac ttttattttt aaaggatttt gttgaatcac
      421 ctggatcgca ttctttttga ccaatgagaa aatgatcatc gaaataattg tatcgatcct
      481 cttttcgagt aggacggcag cttccacggc agtcaatccg cgtttaatcc cttctccttc
      541 cgcttttatg gacggaggga gataactgcc cggaacaata ttatttttct tcttcttaga
      601 agaagaaaaa ctgcgaatca acggaatgat gatcaacaaa ccgacgacaa aaatgaatat
      661 cagggagcca attccccccg aatcgttgtt ttttacttgg gattcggttt ggatttttac
      721 atccggatta aggaattctt taggaaactt tgctccgaat gtatagggcg agctgctgtc
      781 cgcatgatcg gattgccact cgtatacgat atttccttct gctgtaatcc atgaatcggg
      841 ctcatcatct ccgacccaat tctccggtgt gaaataagaa ggatccgaag agctcatacc
      901 gggcggcaat accaacctca cagttaattt tgttgtaccg gtagtgaaat ccgaaccgaa
      961 ataattgggc ataaaacgga aactgacata ttccgtaccg tcacttgaat ccgatgaaag
     1021 atatagaaca ttttttatat tttgaacttc gacataaaca cttgcttttg catttctcgg
     1081 gatcggattt tcggtattat ctatggtaat gccgtacgaa tatccgactt cgtcggggtc
     1141 gacataagag attttatctt gatccactgt ttgatcatca atcttcgcaa ttacatctct
     1201 cagactgaaa ttattattgg gcataccgat atcaatataa tcgatatcct gaccgtagtt
     1261 ttgaatcgta aattcataag aaacagatac cgtcccgtct ttttccaccc agaaaattac
     1321 gagatcctcc ggtattgaaa aacgataatc cgcttggacc gcaaaaattc cgataaagaa
     1381 gatcaaagtt gccaaaaaaa caaagacaaa taatctggcg cctgctttac tcttaccgaa
     1441 aaaatcattc attgttattc tccgccccga ttaccatttc ggctcttccg tgacttcgta
     1501 gacagcaccg caatagggac atttaaccgt cggcgcccca ttgactatcg taaaattgtc
     1561 ggcatttaaa ggtccgccgc atttttgaca actctttgga ttcatcttga tctcgccggg
     1621 gagatctact ttgtagatgt tcgttgtttt tcgattgttt tttgtggtta catatagaat
     1681 aacgataccg atcgccgtag taatcagacc gattatcaaa tcactgctgc tgaaaggggt
     1741 tccgaatgcg ctccatatga aaaggactcc aaaaaaaata catatccctg aaatgatata
     1801 tccgataatt ttcattttct ctccggtttt aagccttttt tattgtccga tttgcacaaa
     1861 agatcagaaa taacgaatga gttgattaaa aatcatgttg tctactgaaa cttaatggca
     1921 attcctcttc acatatatta tataatggaa aaaaaccttt ttatcgaatt aaagaatcag
     1981 ggttttaatc aacgatagaa aagggcatta atctctttct gaaaaaaatc atcaatattg
     2041 aagcttcagt tcgatgtagc ggaggggtat ggaagaaagc gaagatttac ggaaaaaata
     2101 tcttcaattg aaacaagaaa ttaattatca caactatcgt tatcatgttt tggatgatcc
     2161 tgaaatttcc gatgaggcgt tcgataaact gctggtcgaa ttaaggaaaa tagaggagat
     2221 gcatcccgaa tggataactc cggattcccc tacgcaacgt gccggaagtg ctgtttctga
     2281 gaaatttgaa aaagtgactc atcctgttca ggtgttgagt ctggcgaatg cgtttaacaa
     2341 gcaagacgtt atcaattggc tggatcgaac agaaaagatg gatccgagaa taaaatatgc
     2401 cgattttgtc ttggaaccga aaattgacgg tttgacagtt gttttgacat atcaaaatgg
     2461 acttttaata aggggagcta cccgcggaga cggcgttatc ggtgaggatg tcactcccaa
     2521 tattcgaacg ataaagagcg tccccttagc aattccggtt tccgaaaaaa gtgtaaaagt
     2581 tcccgagacc atcgtggtac gcggagaagt ttttattctg gaatccgatt tcgagaaaat
     2641 taatgccgaa ttggctaaaa gaggcgaaaa gacctgggtg aatccaagaa acacggccgc
     2701 tggatcgctg cgccagctga accctgaaat taccgctcaa cgacctttac gaatttttac
     2761 ctatcaaatt cttttttcct ctgccgatca accgattccc gacacacaat ggggaacact
     2821 cgaatatttg caacaattgg gatttcccgt cagccccgat tcaaggtatt gtgagaatcg
     2881 ggaacaaatg ttgaatcact tggatgaatg gcaagagatt cgaaaccgac tcgattatga
     2941 aatagacggt gtcgtcataa aaatcaacga tttgcgcata gccggtagtt tgggctttgt
     3001 gggaaaagat ccgcggggtg ccatcgcatt gaaatttccg gcacgggaag cgacgacgaa
     3061 actgttggat atcggtgtaa atgtcggacg aacaggagtg atcactccct atgcgatttt
     3121 atctccggta gagataggcg
//
LOCUS       DAOMIV010000228         3128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_33206_length_3128_cov_4.575984, whole genome shotgun sequence.
ACCESSION   DAOMIV010000228 DAOMIV010000000
VERSION     DAOMIV010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33206_length_3128_cov_4.575984"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..172
                     /locus_tag="PKV59_06050"
     CDS             23..172
                     /locus_tag="PKV59_06050"
                     /inference="COORDINATES: protein motif:HMM:NF013025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL21 family ribosomal protein"
                     /protein_id="HQP46352.1"
                     /translation="MVDTCSKMVVGEEKGPKLTVFKYRPKKHYRAKTGHRQVYTRIKI
                     EAIEG"
     gene            179..448
                     /gene="rpmA"
                     /locus_tag="PKV59_06055"
     CDS             179..448
                     /gene="rpmA"
                     /locus_tag="PKV59_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQP46353.1"
                     /translation="MAHKKGGGSSRNGRDSNSQRLGVKKFGGELVLSGNILVRQHGTK
                     IKPGLNVGVGRDYTLFATTTGTVVYETLPSGSKRVNVAPLEISEE"
     gene            510..974
                     /gene="rpmE"
                     /locus_tag="PKV59_06060"
     CDS             510..974
                     /gene="rpmE"
                     /locus_tag="PKV59_06060"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQP46354.1"
                     /translation="MKKNIHPVYYTDAKVTCACGNTWTTGSIKKEIRTEVCSACHPFF
                     TGQQQRMLDMEGQVDRFYRRLQARQEFLEEKKSREEAKTSPDRPLEDLELSKRSLDAL
                     AKAGLYKVADVLDKLTSGGDEAMLTIEGFGQKSLIDLKKKLRALGYEVAGKE"
     gene            complement(1034..1690)
                     /locus_tag="PKV59_06065"
     CDS             complement(1034..1690)
                     /locus_tag="PKV59_06065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQP46355.1"
                     /translation="MVNLNEIHDFFQNCFEQYGESPEGVGWNSDSAQEIRFNQLLKVI
                     QPADSFSLVEYGCGYGHLIDYMEKVGYHPSRYYGVDILESAIQAAKKRYGNNPAVSFH
                     RSLDEIPEADYLVASGVFNVKLDHDNVTWTRYIIESLDTFNLIAKRGFSVNFLTKYSD
                     YDRMVQRPDLYYADPCFLFDYCKANFSRNVALLHDYQIYDFTIIVRKEFPEKLTEREK
                     "
     gene            complement(1665..2630)
                     /locus_tag="PKV59_06070"
     CDS             complement(1665..2630)
                     /locus_tag="PKV59_06070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQP46356.1"
                     /translation="MTEEQITQSKQASVVIPVYNGAETIGPLCERLEKVLPALFSDYE
                     IVLVDDGSRDESWERIEYLCTQSGHIRGIRLMRNFGQHNALLCGIREARYPILITMDD
                     DLQHPPEQIPLLLAEFEKGYDVVYAIPKRLPHSWWRNLGSKLTKKVLSRVMHIQIQDI
                     GAFRIFKTELRKAFAKYSSPDVYIDPMLSWGTTKFSHVFVEEEKRPVGHSNYNFGSLV
                     RAALLILTGYSTLPLRFASTVGFVFILFGIGILIYVLIISLTMGSVPGFPFLASIISL
                     FSGVQLFSLGIMGEYISRMYEKSIDRPPYIVAEMKENNGQSQRDS"
     gene            complement(2627..>3128)
                     /locus_tag="PKV59_06075"
     CDS             complement(2627..>3128)
                     /locus_tag="PKV59_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46357.1"
                     /translation="PPGCLRILDPEQHMNLPIFPDKFYEFIPYSHPENILPEGTRSTF
                     LQDEIFKQPVTKNWCYYFQEADLARQYSQWEKIAEIGDQVLNRYQAGEASEYIPFIEA
                     YAQLNRWGDTVPLIEKVHSMNKELDKPLCPILQKLFIEHYPQDQTEREEVASALYRIG
                     CSAYSQ"
BASE COUNT          911 a          661 c          722 g          834 t
ORIGIN      
        1 ctccacggcc tctggctgcc agatcgttga cacctgcagt aagatggtcg taggcgaaga
       61 gaaaggtccg aaattgaccg ttttcaaata tcgtccaaag aaacattatc gagcaaagac
      121 cggacatcgt caggtttata ctcggatcaa gattgaagcg attgaggggt agtaaattat
      181 ggcacacaag aagggtggcg gttcaagccg caatggtcgt gatagtaatt cgcaacgtct
      241 tggcgtaaag aaattcggtg gtgaactggt tttgtcgggc aatattctgg tccgccagca
      301 cggaacaaaa atcaaacccg gcttaaatgt cggtgtcggt cgagactata cgctcttcgc
      361 aaccactacc ggaacggttg tctatgagac gcttccgagc gggagtaaaa gagtcaacgt
      421 tgcgccgctg gaaataagcg aagaataaaa ttgtcactga atcgtcaccg aagcggaatt
      481 aggcgacgaa ttgtgaaagg aaagaaaaaa tgaaaaagaa tattcatccc gtatattata
      541 cggatgcaaa agtaacctgc gcatgtggga atacctggac taccggttcg attaaaaaag
      601 aaattcgtac ggaagtatgt tccgcatgcc atcctttctt taccggccaa caacagcgta
      661 tgttggatat ggaagggcag gtcgatcgat tctacagacg gcttcaagct cgacaagaat
      721 ttttggagga gaaaaaatcc cgtgaagagg caaaaacctc cccggatcgt cctttggaag
      781 atcttgaact cagcaaacgg tcattggatg ctttagccaa agccggtttg tataaagtgg
      841 ccgatgtatt ggataaacta acaagtggcg gcgatgaagc gatgttaaca atcgaaggtt
      901 tcggtcagaa atcattgatt gacctcaaga agaagctgcg cgctttaggc tatgaagtag
      961 ctggaaaaga ataagaattt tcttcgtact aaaaaaatgg ctgcaaaaat gcagccattt
     1021 ttttatccta aaattatttt tctctctcag tcaatttttc tgggaattcc tttcgtacaa
     1081 tgattgtgaa atcataaatt tgataatcat ggagcaaggc aacatttctg gaaaaatttg
     1141 ctttgcagta atcgaacaaa aaacagggat cggcataata aagatcgggg cgttggacca
     1201 tccgatcata atccgagtat ttcgtcagga agtttaccga aaatccccgt tttgcaatca
     1261 gattgaaggt atccaacgat tcaatgatat aacgtgtcca agttacattg tcatggtcca
     1321 atttgacatt aaacactccg gacgcgacca aataatcagc ttccggaatc tcatcaagcg
     1381 atcgatgaaa actgacagcc ggattattcc cgtatcgttt cttcgcggct tgaatggcgc
     1441 tttccaagat atcgacacca taataacggg aagggtgata tccgactttt tccatataat
     1501 cgatcaaatg tccgtaaccg caaccgtatt cgaccaatga aaatgaatcc gcgggctgaa
     1561 tcactttcaa caattgatta aaacgtattt cctgtgcgct atccgaattc caaccgacgc
     1621 cttccggcga ttcgccgtat tgttcaaaac aattttggaa gaagtcatga atctcgttga
     1681 gattgaccat tgttctcctt catttcggcg acgatataag ggggccgatc gatcgatttt
     1741 tcgtacatac gggaaatata ttctcccatg atccccagag aaaaaagttg aaccccagaa
     1801 aataaggaga tgatcgaggc caggaatggg aatcccggga cggaacccat tgtcaatgag
     1861 atgatcagaa catagatcag aatgccgatc ccaaacagaa taaaaacgaa tccgacagtg
     1921 cttgcgaaac ggagcggcaa tgtgctgtag ccggtaagaa tcagtaaagc agctctgacc
     1981 aaggatccga aattgtaatt ggaatgcccg acgggacgtt tttcttcctc gacaaagaca
     2041 tggctgaatt ttgtcgttcc ccatgacagc attgggtcaa tatagacatc cgggctgctg
     2101 tattttgcga atgctttgcg taattcggtt ttgaaaatac ggaaagcgcc gatatcctga
     2161 atttgaatgt gcattactct gcttaaaact ttttttgtca gttttgagcc gagatttcgc
     2221 caccaggaat ggggaagtct tttgggaatg gcataaacca cgtcgtaacc tttttcgaat
     2281 tctgccagca acaaggggat ttgctcaggg ggatgttgta aatcatcatc catggtgatc
     2341 aaaatagggt atcgtgcttc tcggatgccg caaagaaggg cgttgtgctg tccgaaattg
     2401 cgcatcaatc gaattccgcg aatatgaccg ctttgtgtgc agagatattc gattcgctcc
     2461 caactttcat cccgactgcc gtcatcaacc aggacaattt cataatccga aaatagtgcc
     2521 ggcaacactt tttccaaccg ttcgcagagt ggtccgattg tttcggctcc gttatagacc
     2581 ggtatcacaa ccgatgcttg ttttgattga gtgatctgtt cctctgtcat tgagaatatg
     2641 cgctgcaacc gatccggtac aatgccgaag cgacttcttc cctttctgtt tggtcttgtg
     2701 ggtaatgttc gataaagagt ttttgtaaaa tcgggcaaag cggtttgtcc agctctttat
     2761 tcatggaatg cactttctcg attaatggca ctgtatctcc ccaacgattc agttgcgcat
     2821 aggcttcaat aaacggaatg tattctgaag cctctcccgc ctgataccga ttcagaacct
     2881 gatccccgat ttcggcaatt ttttcccact gcgaatattg tctggcaaga tccgcctctt
     2941 gaaaataata acaccaattt tttgtcacag gttgtttaaa gatttcatcc tgtaagaaag
     3001 ttgaacgtgt tccttccggg aggatatttt ccggatgaga gtaggggatg aattcataaa
     3061 atttatcggg aaaaatcggt aaattcatat gctgttcggg atctaaaata cggaggcacc
     3121 cgggaggc
//
LOCUS       DAOMIV010000229         3125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_33255_length_3125_cov_3.730619, whole genome shotgun sequence.
ACCESSION   DAOMIV010000229 DAOMIV010000000
VERSION     DAOMIV010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33255_length_3125_cov_3.730619"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PKV59_06080"
     CDS             <1..861
                     /locus_tag="PKV59_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP46358.1"
                     /translation="GIYGELAMERSQNFSIRKHHIGTHLVLILAVIISIVPFLWMILT
                     SFKTYEESIRIPLTVWPAQWNLINYITVWNKFPFLNFYFNTFFVMVVVIIGELIISSM
                     AAYAFARLNFPFKNAIFIFCLSLMMVPSQIFLIPQYDLMVKAGLNDTLIALILPRLFN
                     VFSVFMLRQFFMTLPRELDDAAKIDGCGFFGIYYQILLPLLKPALVSVAILSGLSTWK
                     DLLWPLIVNNSMEKYTLSAGLALLIGEHTTIYPHVMAGSFLAVWPMILLYFLAQKQFV
                     EGIAFSGIKG"
     gene            complement(947..1033)
                     /locus_tag="PKV59_06085"
     tRNA            complement(947..1033)
                     /locus_tag="PKV59_06085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(997..999),aa:Ser,seq:cga)
     gene            1141..1860
                     /gene="nth"
                     /locus_tag="PKV59_06090"
     CDS             1141..1860
                     /gene="nth"
                     /locus_tag="PKV59_06090"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQP46359.1"
                     /translation="MDEFSSSTHENLPVHKAAVIYDRLIENFGVPNWPKMAPLDEMIN
                     TILSQNTNDRNRDSAFVSLRHRFPTWEAVRDAPASEVIDCIRSAGLGNQKGPRIQEIL
                     RQITEETGGLDLDFLKGWEPERIRTWLTGFKGVGMKTASIVMLFSLGIPAFPVDTHIY
                     RVTGRLGLRPAKMSVEQTHLQMAKLFPPENYGTAHINLILLGRRICFARNPNCSECFL
                     NDLCDYYSEKESIVQKNGTSR"
     gene            1857..2234
                     /locus_tag="PKV59_06095"
     CDS             1857..2234
                     /locus_tag="PKV59_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46360.1"
                     /translation="MKLALILILIGILAFGILRYLRPVILKKISLPDKWDKTLTVAIC
                     ILFPASLFFIFYFLFLGMISWLPASTAEIVWVSDFILMISFGIAGEWLFPTKKKANLS
                     LLCFFIGYALLCLWVIGNTVGTA"
     gene            2280..>3125
                     /locus_tag="PKV59_06100"
     CDS             2280..>3125
                     /locus_tag="PKV59_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HQP46361.1"
                     /translation="MEPISIAKDIYWVGVNDRQSDLFEGLWPITPNGVSLNSYLILDE
                     KKVLIDLASDLLSEHYIQMLEQLIPIHQIDYIVLNHLEPDHTGALLKISSMAPNTIFL
                     GTAKAKEIVGNFYNLTENFRVVADGEELKIGRHTLKFVSIPFVHWPETMVTYDEEDQI
                     LFSCDAFGGYGALPGILFDDQCADLSFMEEEALRYYSNIVAAHSAHTKRAIAKLVNTP
                     IQMIAPSHGLIWRSHPERIFELYSKWANYSDGGREKTATVLYGSMYGNTRFFAESVIQ
                     AVAQKG"
BASE COUNT          797 a          679 c          693 g          956 t
ORIGIN      
        1 ggtatttacg gagagttagc catggaaaga tcacaaaatt tttcaatccg gaaacatcat
       61 atcggaactc atttggtctt aattctggcg gtaattatct cgattgttcc gttcttatgg
      121 atgattctga cttctttcaa gacttacgaa gaaagcatcc gaattccatt aaccgtgtgg
      181 cccgctcaat ggaaccttat caactatata acggtatgga ataagtttcc ttttctcaat
      241 ttttacttca atactttctt tgtgatggtg gttgtcatca tcggagaatt gattatctct
      301 tcgatggctg cctatgcctt tgcacggctg aatttccctt ttaaaaatgc catatttatt
      361 ttttgtctga gcctgatgat ggtcccgagc cagatcttct tgattcctca atatgacctg
      421 atggtcaaag ccggattgaa cgatacgttg atcgcattaa ttctgccgcg attgttcaat
      481 gttttcagcg tcttcatgtt acgacagttt tttatgacgc tgccgcggga attagatgat
      541 gccgccaaaa tcgacggctg cggttttttc ggcatttatt atcaaattct tttaccgctt
      601 ttaaaacccg ctttggtttc ggtggcgata ttatccggac tctctacctg gaaagacctg
      661 ctttggcctt tgatcgtcaa caattctatg gaaaaataca cgctgtcagc cggattggct
      721 ctgttgatcg gtgagcatac caccatctac cctcacgtaa tggccggaag tttcttggcg
      781 gtttggccga tgattcttct gtatttcctg gctcaaaagc aatttgtaga aggaatcgct
      841 ttttccggga taaagggata gaaggattct cgacaggatt tttatcgttt tctccttccg
      901 aaacagaaga gctgaaaatt ctatgataga attttcagct cttctgcgga gaaggtggga
      961 ttcgaaccca cggtagtcaa aagactacaa cggttttcga gaccgccccg ttcaaccact
     1021 ccggcacctc tccatttctt gcttgagcaa ttatactaca tggaatcttc tgtttcttgc
     1081 agtcctttga attctgtccc gtttcctgaa atttgagcct tgtcttataa taacagcgat
     1141 atggatgaat tttcttcctc gacacatgag aacttgccgg tacataaagc cgccgtgatt
     1201 tatgatcgtt tgattgagaa tttcggcgtt ccgaattggc cgaaaatggc gcctctggat
     1261 gaaatgatca acacgatttt gtcgcaaaac acaaacgatc gcaatcgcga ttcggcattt
     1321 gtgagtttac gtcaccgttt ccccacttgg gaggcagtca gagatgctcc ggcttcggaa
     1381 gtaatcgact gtattcgttc agccggactg ggaaatcaga aaggacctcg cattcaggag
     1441 attctcagac agattacaga agaaaccggc ggcttggatt tggatttttt gaagggatgg
     1501 gagcccgaac gcattcgtac ctggttaacc ggttttaaag gggtagggat gaaaacagcc
     1561 tcgatcgtta tgcttttttc gctcggcatc ccggcttttc cggtcgatac acatatttac
     1621 cgggtgaccg gcaggctggg attaagacct gcaaaaatga gcgtcgaaca gacccattta
     1681 cagatggcaa aacttttccc gccggaaaat tacgggacgg cacacattaa tctgattctt
     1741 ctcggacggc gaatttgctt cgcccgtaat ccgaattgct ccgaatgctt tctgaatgat
     1801 ctttgcgatt attattcgga aaaagaatca atcgttcaaa agaacgggac ttcgcgatga
     1861 aactcgcctt aattctgatt ttgatcggca ttctcgcatt cgggatcttg cgatatttgc
     1921 gcccggtgat tcttaagaaa atatcccttc ccgataaatg ggataaaact ttaaccgtcg
     1981 cgatctgtat cctgttcccg gcatcgctct tcttcatttt ttattttctt ttcttgggaa
     2041 tgatttcatg gttgccggct tcgaccgcgg aaatcgtttg ggtgagcgat ttcattctta
     2101 tgatcagttt cggaattgca ggagaatggc ttttcccgac aaaaaagaaa gccaatcttt
     2161 cattgctttg tttttttatc ggttatgctt tgctttgttt atgggtcatc ggaaataccg
     2221 tcggtacagc ctgattatca aggcttagaa tagaattaat ttgattttgg ggaggaatta
     2281 tggagccgat ttcaatcgca aaagatattt attgggtggg agtcaacgac agacagtctg
     2341 atctttttga gggtttatgg ccgatcactc caaacggggt atctttaaac agttatctga
     2401 ttttggatga aaaaaaagtc ctgatcgatc tggccagcga tttattatcc gaacattata
     2461 ttcaaatgct ggaacaattg attccgattc atcaaattga ctatattgtc ctgaatcacc
     2521 ttgaaccgga tcacaccggt gctctcttaa aaatttcaag catggctccg aatacgatct
     2581 tcttgggaac ggcgaaagca aaggaaatcg tcggaaactt ttataacctt accgaaaact
     2641 tcagggttgt ggcagacggt gaagaattga aaatcggccg ccatacttta aagtttgttt
     2701 cgattccgtt cgtccattgg cccgagacca tggtcactta tgacgaagaa gatcaaatcc
     2761 ttttttcctg tgatgcattc ggcggttatg gcgctttacc gggaatcctt tttgatgatc
     2821 aatgtgccga tctttcattc atggaagaag aagcgctgcg atattattcg aatattgtcg
     2881 cggctcacag tgcccatacc aagcgggcga tagcaaaatt ggtaaatacc cccatacaga
     2941 tgatcgcgcc ttctcacggg ctgatttggc gaagccatcc cgaaagaatc ttcgagctat
     3001 actccaaatg ggcgaactac tccgacggcg gacgcgaaaa aacagccaca gttctttatg
     3061 gctccatgta cgggaatacc cgattttttg ccgagtctgt tattcaagct gtcgcacaaa
     3121 aagga
//
LOCUS       DAOMIV010000230         3109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_33488_length_3109_cov_4.637852, whole genome shotgun sequence.
ACCESSION   DAOMIV010000230 DAOMIV010000000
VERSION     DAOMIV010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33488_length_3109_cov_4.637852"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="PKV59_06105"
     CDS             <1..1417
                     /locus_tag="PKV59_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46362.1"
                     /translation="PDTDSIKPIFKNLDIQNAMILAMIDYGTCDIKALTHQKDSVLDG
                     IRVIFKKKDYPEALKFCAEIKKKGYKLSLQPVSVTTYSDSEMTELLKRVNEVAPYSVS
                     IVDTYGLMHKKELLHYFDLMDKNLTDGILIGYHSHNNFQLAYANCTELMAVESDRELM
                     IDGSLYGMGKSAGNACTELLAMHMNENYGGRYNLDQLLEAIDVDIMKEYAKQYWGYSF
                     KHFIAASNDCHPDYVSNLLNKKTLSVRSINEIIAKIEPANKLTFNRDIMENLYLEYQN
                     NIIEDSGAYAGLSTALEQRNILIIAPGKSIETHAEKIRIFIREKNPVIITINFINDDF
                     DPDYVFMGNAKRYSQFFNKIYRKELNVKVICTSNISESNKKIDYKFNFNSLVNEDALI
                     RDNPVLMLLKILLKVKIKEAFLAGFDGYSADNARNYPGEYIQFLYCDDNVVLRNEAVR
                     KALLVIGKGIKLNFLTPTIYV"
     gene            1460..2218
                     /locus_tag="PKV59_06110"
     CDS             1460..2218
                     /locus_tag="PKV59_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HQP46363.1"
                     /translation="MEPVKAVAFDLDGTIYIGDTIVDGVFETLNLLKKKNIQVFYFTN
                     NSAKTRKDIHDKLHKLGLNPAINVVYNTTYATAEYLKKNNFRKVYCCGSSGLQEEMIS
                     AGMACVNNEESPEAVVVGLDHGFNYEKMTIALNILRKKDCAFIICNRDRTYPVENSKL
                     MPGCGPIAAAIECAASRQPDVIIGKPETFMLELLCKDWNLRNKEILIVGDTFDSDIAM
                     AEAYGSPSLLLDMNNECSCKTGKIRSIIEIENYI"
     gene            2296..2742
                     /locus_tag="PKV59_06115"
     CDS             2296..2742
                     /locus_tag="PKV59_06115"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQP46364.1"
                     /translation="MKISWAQLWHEHQIKIRFFIIGVWNTIFGYLAYIGIDYLLTFVF
                     HRRYVAYMTAAVLSNIIATVSAFIFHKYITFKSTVRGKGVIIEFFKFYSTYTVTNVLG
                     LILLPFFVEVFKIDPKIAGALLIPVVAIVSYFGHSRFSFAKKSDDI"
     gene            complement(2757..>3109)
                     /locus_tag="PKV59_06120"
     CDS             complement(2757..>3109)
                     /locus_tag="PKV59_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQP46365.1"
                     /translation="DVYLYAGITGDFNPAHVNEEYAKNTFFKTRIAHGMLTAGFISAI
                     IANQLPGPGTIYLNQNLSFLAPVRIGDTVTGRVEVLELNVEKNRVRIKTTCTNQEGVI
                     VISGEGLVSPPKAS"
BASE COUNT          964 a          659 c          636 g          850 t
ORIGIN      
        1 gcccgatacg gattccataa aaccgatttt caaaaatctg gacattcaaa atgcgatgat
       61 tctggccatg atcgattacg gcacatgcga tataaaagcg cttacccatc agaaagattc
      121 cgttctggac ggcatccggg tcatcttcaa gaaaaaagat tatccggaag cgcttaagtt
      181 ttgcgcagaa atcaagaaaa aaggatacaa attatccttg cagcctgttt ccgtaacgac
      241 ttacagcgac agcgaaatga cagaactttt gaaaagagtg aacgaagtgg cgccttacag
      301 cgtttccatt gtggatacct acggcctgat gcacaaaaag gaattattgc attacttcga
      361 tttaatggac aaaaatctga ctgatggaat tttaatcggt taccattcac acaacaattt
      421 tcagcttgcc tatgcgaatt gtactgaatt gatggcggtg gaatctgaca gagagcttat
      481 gattgacgga tcgctctacg gcatgggcaa aagcgcagga aatgcctgca cggaattgct
      541 ggccatgcat atgaatgaga actatggtgg ccggtataat ctcgatcaac tcctggaagc
      601 cattgacgtc gatatcatga aggagtacgc caaacaatac tggggttatt cgttcaaaca
      661 ttttatcgcc gcctccaatg actgtcaccc cgattacgtg agcaatttac tgaataaaaa
      721 aactttatcc gtaagatcca tcaatgagat tattgcgaaa atcgaaccgg ccaacaaact
      781 gaccttcaac agggatatta tggaaaatct ttatctggag tatcaaaata acattatcga
      841 agattccggc gcttacgcag gactaagcac cgcgctagaa caaagaaata ttctgattat
      901 cgctccggga aaatccatag aaacccatgc ggaaaagatt cgcattttca tccgagaaaa
      961 aaatcccgtt atcatcacca ttaatttcat caatgacgac tttgatccgg attacgtttt
     1021 tatgggcaac gccaaaagat acagccagtt ttttaataaa atataccgga aagagctcaa
     1081 cgttaaggtg atctgcactt ccaacatctc ggaatcgaat aaaaaaatag attacaagtt
     1141 caatttcaat tcgctggtca acgaagatgc tttaatcaga gacaaccccg tactgatgct
     1201 cctgaagatt ctcttgaagg tgaagattaa ggaagcgttt cttgccggtt tcgacggcta
     1261 ttccgccgat aatgcccgga attatcccgg cgaatatatt caattcctct attgcgacga
     1321 taatgttgtc ctgcgtaatg aagccgtgag aaaagcgctt cttgtgatcg ggaaaggaat
     1381 caagttaaac ttcctgactc cgacaattta tgtataacaa agacaagtcg taagaacatt
     1441 ttaacggaga acctggcaaa tggaacctgt aaaagctgtc gctttcgatc ttgatggcac
     1501 tatttacatc ggtgatacaa tcgtagatgg cgtatttgaa accctgaatt tattgaaaaa
     1561 gaaaaatata caggtatttt attttacaaa taattccgca aaaacccgca aagatataca
     1621 cgataaactt cacaagctgg ggttaaatcc tgcgatcaat gtcgtttata acaccactta
     1681 cgcaaccgct gagtatctca aaaaaaataa ttttaggaaa gtttactgct gcggctcctc
     1741 gggtttgcag gaagaaatga tttcggccgg catggcctgc gtcaacaacg aggaatcacc
     1801 cgaagcagtt gttgtgggac ttgatcatgg ttttaattac gaaaaaatga ccattgcatt
     1861 gaatatttta agaaaaaaag attgtgcgtt tatcatatgc aaccgcgacc gaacttaccc
     1921 cgttgaaaat tcaaagctga tgcccggatg cggccccata gccgcggcta ttgaatgcgc
     1981 agcgagcagg cagccggacg tcatcatcgg caaaccggaa actttcatgc tcgagcttct
     2041 ctgcaaagac tggaatttac gaaacaagga aatactcatt gttggagata catttgatag
     2101 tgacattgcc atggctgaag cctatggttc accatcgctt ttactggata tgaataatga
     2161 atgttcatgt aaaaccggga aaattagaag cataattgaa atagaaaatt atatctgata
     2221 taaaaatcat cgcatacttt ataagcatca acaaaactga agatatttta ttattcaaat
     2281 catggggcat atcttttgaa aatatcctgg gcacaattat ggcatgaaca tcagattaaa
     2341 atccgctttt tcatcatcgg cgtctggaat acgattttcg gctacttagc ctacatcggc
     2401 attgattacc ttctgacatt cgtttttcac agacgttacg tggcttatat gacggcggcg
     2461 gttctgtcga acatcatcgc caccgtcagc gcttttattt ttcacaagta cattactttt
     2521 aaatcaacgg tcaggggcaa aggtgtcatc attgaattct tcaaattcta ctcgacctac
     2581 accgtaacca atgttctggg cctaatcttg cttccatttt tcgtcgaggt cttcaagata
     2641 gatcccaaaa tcgccggcgc gcttttaata cccgttgtag caatcgtcag ttatttcgga
     2701 cattcaagat tttcatttgc caaaaaatct gacgacattt aatccattga gcctcctcaa
     2761 gaagctttcg gcgggcttac taatccttcg cctgaaatca cgataacgcc ttcctgattg
     2821 gtgcaggtgg ttttaattct aacacggttt ttctctacat tcaattccag aacttccacg
     2881 cgtccggtga cagtatcgcc gatgcgtact ggcgcaagga agcttaaatt ttgattcaaa
     2941 tagatagtgc cgggtccggg caactgattg gcgatgatgg cagaaataaa tccggcggta
     3001 agcatgccat gcgcaatgcg tgtcttgaaa aaagtatttt tggcgtattc ttcgttgaca
     3061 tgtgccggat tgaaatcgcc ggtaataccg gcatataaat aaacatctg
//
LOCUS       DAOMIV010000231         3107 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_33511_length_3107_cov_4.252621, whole genome shotgun sequence.
ACCESSION   DAOMIV010000231 DAOMIV010000000
VERSION     DAOMIV010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33511_length_3107_cov_4.252621"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..350)
                     /locus_tag="PKV59_06125"
     CDS             complement(225..350)
                     /locus_tag="PKV59_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46366.1"
                     /translation="MKKSHDSKQTSFGRIGDAALGRQGNKDQRNAMVIGLVVTEK"
     gene            complement(524..613)
                     /locus_tag="PKV59_06130"
     tRNA            complement(524..613)
                     /locus_tag="PKV59_06130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(575..577),aa:Ser,seq:gga)
     gene            722..1735
                     /locus_tag="PKV59_06135"
     CDS             722..1735
                     /locus_tag="PKV59_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46367.1"
                     /translation="MYIQNSQIPQPILSVSQKTLDFGLVSAGEPAEGHGIAKLTITND
                     GDRILVGRIAIQVAWVTVDPPDFRLNPGESSEHIFTVRQNVQFSWTTRRMGSDFIALI
                     NSNGGSETIGGYYFLDSTKTEKKKKPIHPWLYALIPVFILLTAGIILFVSRQNEAALA
                     QQKTENSDSLYTRIAETYFAEMTENPTIISTEPPPQNTPNPLVVPNFDITPSPAPSMT
                     FTPWVAADYPNVEEFIRTYYKTLNERDYETAWWMLSEKMQQACCYTGEANPMTVYQNY
                     WDKVESVEVNYAFLQSYDTNPAIVNVGLTYHYKDGTTEETVNQFSIITDDVKKSLVID
                     EIR"
     gene            1811..2038
                     /locus_tag="PKV59_06140"
     CDS             1811..2038
                     /locus_tag="PKV59_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQP46368.1"
                     /translation="MEITLDNVKKVITDVLKIDGSDITPETRFVEDLKADSMDQFFLI
                     DGFSEAFNVTISDEQAKEIKTVGDVIRVLSK"
     gene            complement(2101..2967)
                     /gene="sucD"
                     /locus_tag="PKV59_06145"
     CDS             complement(2101..2967)
                     /gene="sucD"
                     /locus_tag="PKV59_06145"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HQP46369.1"
                     /translation="MGILINSSTKVIVQGITGREGSFHTEQMAAYGTQIVAGVSPQKG
                     GSWVLDGKIPVFDTMQAAMEVTEANASIIFVPARNATDALFEAADSGVELVVCITEGV
                     PVQDVVKVCQYMSNRGVRLVGPNCSGIINPGEAKLGVAPSDCVIPGKVGVISRSGTLS
                     YLVMSEMKSRGIGVSTCIGIGGDIVQGTTFVEALELFETDPYTERILLIGEVGGLEEE
                     RAASYVAYKMTKPVTALLVGKTAPQGKKMGHSGAFIEAGIGGIDSKIEAFESVGVRVV
                     TDIKEVPDALQG"
BASE COUNT          829 a          785 c          651 g          842 t
ORIGIN      
        1 taggataacc cgtagagaat ttccatagcg tttttgtact ccgcacttga gttgggatct
       61 cctttaccgt caatcatcag aaatgccatc tccggtatat caatgatttc cggctttgtt
      121 tttggtaaat aaaactgttt ttccgttttc ttataatcaa ctgtcactat attttctcct
      181 gtttcggcaa attggtcata aatgatccga aaatgctctt tgatttattt ttctgtcacg
      241 accaaaccga ttaccatcgc gttccgctga tctttattcc cctgtcgtcc aagtgctgca
      301 tctccgattc taccaaagga agtctgcttc gaatcatggc tttttttcat tttataaccg
      361 gagcaaaaaa caccttactt gtccgacgac gtacgaatta tttgccgttg ccggtttttt
      421 gagcctatac cgagagcgat tgttttttct gtccgtcgga aaattatgaa tcaaaaaacc
      481 atccatcgac aagttcattc ctgccaacag acggttttca atctggcgga gagagtggga
      541 ttcgaaccca cgttaccttg cggtaaacgc gctctccagg cgcgcgcgtt aggccagact
      601 acgctatctc tccctaatgt tgcggacgct attataccat aaggtaaatt attttaggaa
      661 aaccccaaga tttcagatag aaaataacct gttttataaa agtgtagact ataatcgaat
      721 tatgtacatc cagaattcgc aaattccgca gccgatcctg tctgtatcac aaaagaccct
      781 cgatttcgga cttgtctcag ccggtgaacc ggcagaggga cacggtatcg ccaaattgac
      841 gatcacgaat gacggagatc ggattctggt cgggaggatt gcgattcagg tcgcttgggt
      901 caccgttgac ccgcccgatt ttcgcctgaa cccgggtgaa agcagcgaac atatttttac
      961 agtgcgccaa aatgttcagt tctcttggac aactcggaga atgggttcgg attttatcgc
     1021 cctgatcaac agtaatggcg gatccgaaac gatcggcgga tactattttc tcgattcgac
     1081 aaaaacagag aagaaaaaga agccgattca tccctggctg tatgcactca ttccggtttt
     1141 cattttgctg acagccggaa tcattctttt tgtttcgcga caaaatgagg cggctctggc
     1201 acagcaaaaa accgaaaact ccgattcgct gtatacccgg attgcggaaa cctattttgc
     1261 cgaaatgaca gaaaatccga ccattatttc caccgaacct cctccgcaaa atacccccaa
     1321 tccgctggtt gttccgaatt tcgatattac tccttctccc gctccttcga tgacgtttac
     1381 tccttgggtc gcggcggact atccgaatgt cgaagaattc atccgcactt actacaaaac
     1441 gctgaatgaa cgagactacg agaccgcttg gtggatgctt tcggaaaaaa tgcaacaagc
     1501 ttgctgttac acgggagaag caaacccgat gacggtttat caaaattact gggataaagt
     1561 cgaatccgtt gaggtaaatt atgcgtttct ccagagctat gataccaatc cggcaatcgt
     1621 aaatgtcggc ttgacttatc attacaagga cggaacgacc gaggaaacgg ttaatcagtt
     1681 ttcgattatc acagatgatg tcaaaaagag tttggtgatc gatgaaatca ggtaaaattt
     1741 ttatcagtca agtgagtgtt aaaatcgata acaaaatatg aattgagaaa tttattcagg
     1801 agaacgaatt atggaaataa ctttggataa tgtaaaaaaa gtgattaccg atgtattaaa
     1861 aatcgacgga tcggatatca ctcccgaaac ccgttttgtc gaagatctta aagcggattc
     1921 aatggatcag tttttcctga tcgacgggtt cagcgaagct tttaatgtga ccatttccga
     1981 cgaacaggcg aaggaaatta aaaccgtggg agatgtcatc agagttctgt ccaaataaat
     2041 cgaaaaaggc tgataaacag gaacatctgc ctgtcttctg caatgttcct gtttttttat
     2101 ttaaccctgt agggcgtcgg ggacttcttt aatatcggtg accacacgga caccgaccga
     2161 ttcgaacgct tcgattttag agtcgatacc gccgattccg gcttcgatga aggcgccgga
     2221 atgtcccatc tttttcccct gcggagcggt tttccccacc aacaatgcgg tgacgggttt
     2281 ggtcatttta taggcgacat aactcgccgc acgttcctct tccaatcctc cgacttcacc
     2341 gattaaaaga attcgctcgg tatatggatc ggtctcaaac agttccaacg cttccacaaa
     2401 ggtagtcccc tgaacaatat ctccgccgat cccgatacag gtactgacac cgattccccg
     2461 acttttcatt tcactcatca ccaaatagct gagcgttccg gaacgcgaga taacgccgac
     2521 ttttccggga atcacacaat cggacggcgc tacccccaat ttggcttccc ccgggttgat
     2581 aatgccggaa cagttcggcc cgaccaatcg cactccccgg ttggacatat attggcacac
     2641 ttttaccaca tcctgcaccg gaactccttc ggtgatacaa accaccaatt ccacgcccga
     2701 atccgccgcc tcaaacagtg catcggtggc gtttcgcgcc ggcacaaaga tgatcgatgc
     2761 attcgcttct gtgacttcca ttgcagcctg catggtatcg aataccggga tttttccgtc
     2821 cagcacccag cttccgcctt tttgaggcga gacacccgca acaatttgag tgccgtaagc
     2881 cgccatttgt tccgtatgaa agctgccttc cctgccggtt attccttgaa caatgacctt
     2941 cgtcgatgaa ttaatcaaaa ttcccatatc aaccctgtcc cgcagccgtt tcgaccgctt
     3001 ttcggatcgc ttcttcataa ctcggcacca aaatcgccgg agagccgctt aagatttgat
     3061 tccccaatgc cgacatcgac cctcccatat agacaacgat gggaact
//
LOCUS       DAOMIV010000232         3096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_33698_length_3096_cov_4.328839, whole genome shotgun sequence.
ACCESSION   DAOMIV010000232 DAOMIV010000000
VERSION     DAOMIV010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33698_length_3096_cov_4.328839"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..2078
                     /locus_tag="PKV59_06150"
     CDS             438..2078
                     /locus_tag="PKV59_06150"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQP46370.1"
                     /translation="MNHYSLKLNQCLTVLPEIRSISGNKPDITYEDITFDSRKVTPGS
                     IYVALKGINTDGHNFIPNAIQNGAVAVIGSNDMPTELPVPYIRVENPRLAMAYAAAAL
                     YRFPSNEMAVIGVTGTDGKTTTATLMYHILRKAGLKAGLISTVSAIINGREIDTGFHV
                     TTPESPDIQRYLAQMRDEGITHVVCEVTSHGLSQNRVAAIDFDIAIVTNITHEHLDYH
                     KTKEEYFAAKGMLFKALGQHPRKLAPLAVLNYDDQASYTYLSDIIPNTHKICYSASGE
                     ELVSQVSLKKVVTFSKGLKAEILFRNLPDHTEDRIVSVQSNLIGTYNCANILAAMTAA
                     VYGLGISPEKAAQGVQEVSAISGRMEVIDCGQNFTAMVDFAHTPNALAVALDSARAML
                     PISEDSGIPPNRIIAVYGSAGLRDREKRRMMPAVSVEKADITILTAEDPRTESLDMIL
                     KEMADEAVKHGAVMGENLFIEPDRGNAIRLGLSLAKPGDIVISFGKGHEQSMCFGTVE
                     YAWDDRTAMRAALCEMLNKEGPQMPFLPTSNRETQIQK"
     gene            2109..2546
                     /locus_tag="PKV59_06155"
     CDS             2109..2546
                     /locus_tag="PKV59_06155"
                     /inference="COORDINATES: protein motif:HMM:NF021463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HQP46371.1"
                     /translation="MSKNKLFLFCELTIGIILAVVSFVFRKDMPVIIAGILIAAGIGL
                     IGNSISQLMMDHFEKKNPEIIREDTPFTTDERNSLIRYKAKAKTSDITQWLIIGMALI
                     SVLTKAPVWAIILFALVYLAHLLMPYYFMVRYRNEMKNNSPES"
     gene            complement(2571..>3096)
                     /locus_tag="PKV59_06160"
     CDS             complement(2571..>3096)
                     /locus_tag="PKV59_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HQP46372.1"
                     /translation="SYIPEERNKDGVIKDFNISENFILEDHDKEPYSKHTFFNFANIA
                     RKTQKAVDDFAVKTPNLKTFVKNLSGGNVQKLILARELSRNPSVLIAAQPTRGVDIGA
                     SEYIHQRLLKQRDQGTATLLISEDLDEIRALSDRIAVIFEGKIMGIVDAATATSEQLG
                     LMMAGIPQEEAMKQ"
BASE COUNT          862 a          688 c          700 g          846 t
ORIGIN      
        1 taatgcatct cgatttcatt gttgggtatg gtgtcatgca atgcatgcca tatttttgag
       61 gacgctgtcc cgtaaaagtg tgatttaaat ctgcaaatat caccctcacc ctaccctctc
      121 ccgatgggcg agggaatttc gtcatcctgc cgcttatcat cccatctggc atcgccctct
      181 cccgatgggc gagggaattt tcattagaga cggcatcctt gcgaagcacg ccgaggtgac
      241 gaggtgcgac gtcctgtacc gaacatactc gacttttaaa agatattgtt tttatcctgt
      301 caaaaagcct ttaaagacag gacaatcttc ttgagaaaaa atgtttttaa aataatcgcc
      361 ctgaattgtt gccgaaaaga caaagtgaat ttggtaaaat aagaacaata aaataaatac
      421 aagggggcaa aactgtaatg aatcattaca gcctcaaatt aaaccaatgt cttaccgttt
      481 taccagaaat cagaagcatc tcaggcaaca aacccgacat cacctatgaa gatatcacct
      541 ttgattccag gaaagtcacc ccgggaagca tctacgtcgc tttgaaaggg atcaataccg
      601 acggccataa tttcatcccc aatgccattc aaaacggcgc cgtcgccgta atcggttcga
      661 atgatatgcc gaccgaactt ccggttccat acatccgtgt agaaaatccg cgcttggcaa
      721 tggcttatgc cgccgcggct ctctatcgtt ttccttctaa tgaaatggcg gtgatcggtg
      781 tcaccggaac agacgggaaa acaaccacag ccacgttaat gtatcatatt ttgaggaaag
      841 ccggcttgaa agccgggttg atctcaacgg tatcggcgat tatcaacggt cgtgagatcg
      901 ataccggttt tcatgtgacg acaccggaaa gtccggatat ccaacggtat ctggcacaaa
      961 tgcgagacga agggattact catgtcgttt gtgaagtcac ctctcacgga ttgtcgcaga
     1021 atcgagtggc tgcgattgat tttgatatcg caattgtgac aaacatcact cacgaacatt
     1081 tggattacca taaaacgaaa gaagaatatt ttgcggcaaa gggcatgtta ttcaaggctc
     1141 ttggtcagca tccgaggaaa ttagcgccgt tggcggtctt gaactatgat gatcaagctt
     1201 cttataccta tttatcggat atcattccca atacgcataa aatctgttat tcagcctcgg
     1261 gagaagaact tgtctctcag gtgagtctta aaaaggttgt caccttttcg aagggattga
     1321 aagcggaaat tctgtttcga aatctcccag atcatacgga agaccgaatt gtttcggttc
     1381 aatccaattt gattggtact tataactgtg caaatatttt ggcagcgatg accgcagccg
     1441 tttacggatt gggcatatca ccggagaaag cggcgcaagg cgttcaggag gtttcggcaa
     1501 tttcgggccg catggaagtg atcgactgcg gtcagaattt cactgcgatg gttgattttg
     1561 cccatacgcc gaatgcattg gctgtcgctc ttgattccgc ccgcgcaatg cttccgatat
     1621 ccgaagattc cggcattcca ccgaatcgaa taatcgctgt ttacggatca gccggattac
     1681 gtgatcgtga aaaaagacgg atgatgcccg cggtatccgt tgaaaaagcc gatataacca
     1741 ttctgactgc cgaagatccc cgtaccgaat cgctggatat gatcctaaag gaaatggcag
     1801 acgaagcagt aaaacacggt gcggttatgg gcgaaaacct gtttatcgaa cccgatcggg
     1861 gaaatgctat tcggctggga ctttcgctgg cgaaaccggg agatatcgtt atttcattcg
     1921 gaaaaggcca tgaacaatcc atgtgcttcg gaacggttga atatgcttgg gatgatcgaa
     1981 cagctatgcg agcggctctc tgtgaaatgc tgaataaaga gggacctcag atgcctttcc
     2041 ttccgacttc aaatcgtgag acgcaaattc aaaaataaca tccgtccgtc tgtgttgaaa
     2101 ggaatgaaat gtctaagaac aagctttttc tattctgtga attaaccatc ggaattattt
     2161 tggctgtcgt atcttttgtt tttcgaaagg atatgccggt tatcattgcc ggcattttaa
     2221 tcgcagcggg tatcggcttg ataggaaaca gtatcagtca gttgatgatg gaccatttcg
     2281 agaaaaagaa tcccgaaata atccgagagg atactccttt cacgaccgat gaacggaaca
     2341 gcctgatccg atataaagcg aaagcaaaaa cgagcgatat aacgcaatgg cttattatcg
     2401 gaatggcatt gatttcggta ttgacgaagg ctccggtttg ggcaattatc ctatttgcct
     2461 tggtttatct tgctcattta ttgatgccgt attattttat ggttcgctat cggaacgaaa
     2521 tgaaaaataa ttctcccgag tcctgaaatg tcgaccgaaa cgggtgcggc ctattgtttc
     2581 atcgcttctt cctgaggaat acccgccatc atcagcccca attgttctga tgttgcagtg
     2641 gctgcatcga cgatccccat gatttttcct tcaaagataa ccgcaattcg atcggataat
     2701 gcccgtattt catccagatc ttccgaaatc aacagggttg ccgttccctg atcccgttgc
     2761 ttcagcagcc gttgatgaat atattcggaa gctccgatat ccactccccg agtgggctga
     2821 gcggcaatca aaactgaagg attccggctg agttcccgtg ccaaaattaa tttctggaca
     2881 tttcctccgg aaagattctt gacgaaagtt ttaagattcg gtgtttttac ggcaaaatca
     2941 tcaaccgctt tttgagtttt acgggcaata ttcgcaaaat tgaaaaaagt gtgctttgaa
     3001 taaggttctt tatcgtgatc ctctaaaatg aagttttcgg aaatattaaa atccttgatc
     3061 acaccatctt tatttctctc ttcgggaata taagat
//
LOCUS       DAOMIV010000233         3092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_33765_length_3092_cov_4.469213, whole genome shotgun sequence.
ACCESSION   DAOMIV010000233 DAOMIV010000000
VERSION     DAOMIV010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33765_length_3092_cov_4.469213"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PKV59_06165"
     CDS             complement(<1..296)
                     /locus_tag="PKV59_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP46373.1"
                     /translation="MTDLLTINNLVKHFPAEKKIFGKQDYVHAVNNVSLEVREGETFS
                     VVGESGCGKSTVSRLINHLIQPDSGEIWFDGTETAHLNENEFRPFRQHMQMIFQ"
     gene            complement(293..1309)
                     /locus_tag="PKV59_06170"
     CDS             complement(293..1309)
                     /locus_tag="PKV59_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46374.1"
                     /translation="MSENNLLKVENLRTEFKISRKKSVAAVSDITFEINKGEIVGLVG
                     ESGCGKSVTSLSIIRLLNDTSGEITHGSVWFKGSNLLKLSDEEMRQIRGKEIAMIFQE
                     PMTSLNPVLRIDNQLIEAIQLHTEYTKQEAREHALETLRSVGIPDPERVSRCYPHQLS
                     GGMSQRVMIAMAMSCYPDLLIADEPTTALDVTIQAQILDLMQGIRNTRGTSILLITHD
                     LGIVAETCSRVYVMYAGRIIEEAPVDILFSDPSHPYTQGLIASIPKLGLEAESLPFIP
                     GSVPDITMMPGGCRFHPRCRYATEICSQKEPELISINESQKCRCWLKTSEYSIKTENG
                     ESRK"
     gene            complement(1321..2199)
                     /locus_tag="PKV59_06175"
     CDS             complement(1321..2199)
                     /locus_tag="PKV59_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQP46375.1"
                     /translation="MQTSTFIDQPIKKAENRTKYYFRKFFQRKTAVIAAVIILIFLFF
                     AIFGPQIAPFDPSLPDYDNLYGAPDAVHWFGTDEFGRDVLSRLLVGTRNSLGVSLASV
                     LIGAAIGTFLGVLAGYYGGIIDSVVMRGSDILFAMPDILLAIAIVAIIGPGIINVVIA
                     VAVFTIPSFARIMRSATLSVKSSLYVEAARSIGCKNSRILWVHIFPSTIQSMIVNFTM
                     RIGTAILAAASLSFLGYGAKVDEPDWGAMLSSGRNYLDIAPHLVYFPGLLIFIAVLAF
                     NLLGDGLRDTLDPKLK"
     gene            complement(2212..>3092)
                     /locus_tag="PKV59_06180"
     CDS             complement(2212..>3092)
                     /locus_tag="PKV59_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQP46376.1"
                     /translation="LLLVISFLIFMFIHLIPGDPVRNMAGREATTEELENLRESMGFN
                     KPLYQQYFSYLNDLFHGDLGISYKTKLPVSEMMIGRLKPTLKLTFFAMLWSTIVGIAI
                     GVLSAINRGKLIDYLGMLIAISGISIPGFWLGLVMIQIFSVKLGWVPTGGLDTWKSYF
                     LPSITLGCGIMAILARFSRSSMLDSLGEDYVRTARAKGQRERLVILRHAFRNSLVQVV
                     TVIGLQIGGLLAGSVLIETVFTIPGLGRLLVDSLAFRDYKVVQALLLFFSFEYILINL
                     IVDLLYGVLNPRIRLK"
BASE COUNT          869 a          747 c          703 g          773 t
ORIGIN      
        1 tcttggaaga tcatctgcat atgttggcga aaaggccgaa actcattttc attgagatgt
       61 gccgtttccg ttccgtcaaa ccaaatttca ccggaatcgg gttgaatcag atggttgatc
      121 aaacgactga cagtcgattt cccgcaaccc gattcaccga cgacggagaa cgtttcgcct
      181 tcccggacct ccagagaaac gttattcacc gcatgaacgt aatcctgctt tccgaaaatt
      241 tttttctccg ccggaaaatg ttttaccaga ttgttgattg tcagcaaatc agtcattttc
      301 tgctttctcc gttctcggtt tttatggaat attcggatgt tttcaaccag catcgacatt
      361 tttgcgattc attgattgag atcaattcgg gttctttttg gctgcagatt tccgtggcat
      421 aacggcagcg gggatgaaac cgacatccgc ccggcatcat ggtgatatcc ggtacactcc
      481 ccggaataaa cggcaggctt tccgcttcca accccaattt cggaatggac gcgatgagac
      541 cttgtgtata gggatggctc ggatcggaaa aaaggatgtc taccggtgct tcctcgataa
      601 ttcttccggc atacatcaca taaacacggg aacaggtctc ggcaacgata cccaaatcat
      661 gtgtgatcaa taagatgctt gtgccgcggg tatttcgaat tccctgcatc agatcgagga
      721 tctgagcttg gatcgtgaca tccaatgcgg tggtcggttc gtctgcgatc aacaaatccg
      781 ggtagcagga cattgccatc gcaatcatta cccgctggct cattcctccg gataattggt
      841 ggggatagca gcggctgaca cgctccggat cgggaattcc gacacttctt aacgtttcca
      901 acgcgtgctc acgagcttcc tgttttgtgt attcggtatg aagttgaatc gcttcgatga
      961 gttgattatc aatccggagg acgggattca atgaagtcat cggttcctga aaaatcatcg
     1021 cgatttcttt cccgcggatt tggcgcattt cctcgtcgga taatttcagt aaattgcttc
     1081 ccttgaacca gacggatcca tgggtaattt cgccagaagt atcatttaac aaacggataa
     1141 tcgaaagcga tgtgactgat tttccgcagc ccgattctcc gaccaaaccg acaatctcac
     1201 ctttgtttat ttcaaaggtt atgtcactga cggctgcaac ggactttttc cggctgattt
     1261 taaattcggt cctgagattc tcgactttca gaaggttatt ttcgctcatt tttctgtgtc
     1321 ctatttcaac ttcggatcca acgtatcgcg cagtccgtcc ccgagaagat taaatgccag
     1381 aaccgctata aaaatcagca gcccggggaa atagaccaaa tgcggagcga tatccaaata
     1441 atttcgtccg gaagaaagca ttgctcccca atccggttcg tcgacttttg ccccgtaacc
     1501 gaggaaactg agggaggctg ccgcaaggat cgcagtgccg atacgcatcg taaaattgac
     1561 gatcatagat tgaatcgttg acggaaaaat atgtacccat aaaatacgac tgttcttaca
     1621 gccgatggaa cgagcagctt ccacatacag agagcttttg acggacaagg tggcactccg
     1681 catgatccga gcaaaagaag gaatggtgaa caccgcgacg gcgatgacaa cattgatgat
     1741 tccgggaccg attattgcaa caattgcgat cgccagcaaa atatccggca tggcaaacag
     1801 gatgtccgag ccccgcatca caacggaatc gatgattccg ccgtaatatc cggcaaggac
     1861 tcccaaaaat gtgccgatgg cagctccgat cagaacggaa gccaaggaaa cacccaatga
     1921 atttcgcgtt ccgaccaaca aacggctgag aacatcgcgt ccgaattcgt ctgtcccaaa
     1981 ccaatggaca gcatcaggag ctccgtatag gttatcgtaa tccggtaaag aaggatcaaa
     2041 aggagctatc tgaggaccaa aaatagcaaa gaacaggaag atcaggatga taaccgccgc
     2101 gatgaccgct gttttccgct ggaaaaattt acgaaaatag tattttgttc tgttttcggc
     2161 cttttttata ggttgatcga tgaaggttga tgtctgcatg gtcttttcct gttactttaa
     2221 tcgaattctc ggattgagga caccataaag caggtcgaca atcagattaa tcaggatata
     2281 ttcaaaagag aaaaacagta ataatgcctg aacgacttta taatcgcgga aagcaagaga
     2341 atcgaccaga agccttccca atccgggaat cgtaaacacg gtttcgatta ataccgatcc
     2401 ggccaagagc ccgccgattt gaaggccgat aaccgttaca acctgtacca aagaattccg
     2461 gaaagcatgg cgcagaatga ccaatctttc acgttggcct tttgcccgag cggtccgcac
     2521 ataatcttcc ccgagtgaat ccagcatcga tgatctcgaa aaacgtgcga gaattgccat
     2581 aataccgcat cctaacgtga tggatggcag gaagtaactt ttccaggtat ccaaccctcc
     2641 ggtcggaacc cagcccagtt tgacggaaaa gatttggatc atgacgagac cgagccaaaa
     2701 ccccgggatg gaaattccgg aaatcgcgat gagcattccc aaataatcga tcagttttcc
     2761 tctatttatg gcggaaagga cgccgatggc aattcccacg atagttgacc agagcatggc
     2821 aaagaaagtc agttttaatg tgggctttaa ccggccgatc atcatctccg aaacgggcaa
     2881 tttcgtctta tacgaaattc ccaaatctcc atgaaaaaga tcattcaaat acgaaaagta
     2941 ttgctgataa agcggcttgt taaaccccat gctttcccgc aggttttcga gttcttcggt
     3001 tgtcgcttct cgacctgcca tatttcgtac cggatcaccg gggatcaaat gaataaacat
     3061 aaagatcaaa aaagaaatta ccaaaagcaa cg
//
LOCUS       DAOMIV010000234         3087 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_33846_length_3087_cov_2.930409, whole genome shotgun sequence.
ACCESSION   DAOMIV010000234 DAOMIV010000000
VERSION     DAOMIV010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33846_length_3087_cov_2.930409"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PKV59_06185"
     CDS             <1..1028
                     /locus_tag="PKV59_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46377.1"
                     /translation="SAMRIVPLIFVVIFLIYWFKLPKNSRLNALKAFLILTGFAILFC
                     LPLIRIATVSPEMVFYRTLTRLSDAETSISASPIAIFFSNLWKALIMPFWDNGRIWVH
                     SIPFRPALDYLSASFFFIGLVLIILRIIRDKRWQDIVLILSISLLMLPSVLSIAFPDE
                     NPCLNRTGAAAIPILITAAYGIVSVCNSLISRFKESKINILFTAVLGIAFLFAIGKNN
                     YDLVFNEYRQNYDLNALNTRQIGQVIRGFADSVGSADDAYVIPYPYWVDTRLVGINAG
                     FPRKDYALSRDLIGSVVKGDSPVLFIYKDNDSETAAILKKLYPDGLEKLYEGKVSGKN
                     FYGYLVP"
     gene            1056..2567
                     /locus_tag="PKV59_06190"
     CDS             1056..2567
                     /locus_tag="PKV59_06190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP46378.1"
                     /translation="MNLGKGDQRKPIYFLWLVPFILGIFFRFYGLNWDNGQFLHPDER
                     FLVQVADSIHFPASISLWFNAAESSFNPSNSGFHFYVYGTFPLVLIKAIQGFLGHYTD
                     PASYSFLLLTGRFLSAFFDFLTLLCICRFSRNLWKSRKAAFLSVCGYGFFVGAIQQAH
                     FFTVDSFCTFFCTFSLLSVLGDRKKHFYSQAIVSGIALGIALACKISAILFILFPALD
                     MVFRTVRRHSDIKKELFRFFLMISASAFTFRIFQPYAFQNPQFFDFRLNSSWIAAMRE
                     LAEQSSGRVDFPPAWQWVGRSPFYALKNLLIYGMGLLPGGLVFAGFVLSIRQGFKKDN
                     YVILTLIIGTAVFFLAQTAQFSKMMRYLYPICPILFLLLGSLLEVEGKVWMKIFLSTA
                     LACAFLRSCAFLSIYQRPHTRVQASRWVYDQIPAAISVQVSGETDEVEPDLQVAVPTI
                     FSVETGDDAIQIPLAVQKGDIPRLIEFENLKVNGENCSIRASINSTEENRDLF"
     gene            2564..>3087
                     /locus_tag="PKV59_06195"
     CDS             2564..>3087
                     /locus_tag="PKV59_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46379.1"
                     /translation="MTGDLIPNESQILFAGDDAILGNQSFLSLQTIGNGCSAEISGDS
                     FLYFLRNGKPRYLLLKGLAAAWITDDKPLEVHFKIQDDGSIAGLSFHSMMFSGGMDQS
                     VPISFTFINYNNSTEKTENFSIIDSTNQPVAFSLSDPIKVKAGEMIGLRIAVNSERAI
                     SVQPKRIATESSWD"
BASE COUNT          745 a          618 c          702 g         1022 t
ORIGIN      
        1 acagtgcgat gcggattgtt ccgttaatat ttgttgtgat cttcctgatt tattggttca
       61 aacttcccaa aaattcccgt ttgaatgcat taaaagcctt tttgatattg acgggatttg
      121 cgattctttt ctgcttaccg ctaattcgca tagcaacggt ttctcccgaa atggtttttt
      181 atcgaacact taccagactc agtgacgcag aaacatcgat cagtgcttca cctatcgcta
      241 tctttttttc aaatctgtgg aaagcactga taatgccttt ttgggataat ggcaggatct
      301 gggttcattc gatcccgttt cgcccggcgc tggattattt gagcgcttct tttttcttta
      361 tcggactcgt cttaatcatt ctgcgaatta tccgggataa gcgatggcag gacatcgtgt
      421 tgatcctttc catttcgctg ttgatgttgc cttctgtttt gtctatcgcg tttccggatg
      481 aaaacccgtg tttaaatcga accggagcgg cagcaatacc gattttgata acggctgcct
      541 atgggatcgt ttcggtttgc aattcattga tttcgcgttt taaggaaagc aaaataaata
      601 tcctttttac ggcggtgctc ggaatcgctt ttctctttgc tatcgggaag aataattatg
      661 atttggtttt caatgaatat cggcaaaatt atgatttaaa tgctttaaat acccgtcaaa
      721 tcgggcaggt tatcagggga tttgcggact cggtcggctc agcagacgat gcatatgtca
      781 tcccttatcc ttattgggtc gatacaaggt tggtgggaat taatgccgga ttcccgcgaa
      841 aagattatgc attgagtaga gatttgatcg gttcggtcgt taaaggggat tcgccggtac
      901 tgtttattta taaagacaat gattctgaaa cggcagcgat tttaaaaaag ctctatccgg
      961 acggtttgga aaaattatat gagggaaaag tctccggtaa gaatttttac ggttatttgg
     1021 ttccgtagag gcttgaataa ccggaagaga ttcgtatgaa cctcggaaag ggcgatcaaa
     1081 gaaagcccat ctatttttta tggcttgtcc cgttcatact cggcatattt ttcagatttt
     1141 acgggttaaa ttgggataac ggtcaatttc ttcatccgga tgaacgtttt ttagtacaag
     1201 tcgcggacag cattcatttt ccggcttcga tttcgctctg gttcaatgcc gcggaatcct
     1261 cttttaatcc ttccaacagc ggttttcatt tttatgttta tggaactttt ccgctggttt
     1321 tgattaaagc cattcaagga ttcctcggtc attacacgga tccggcctcc tattcatttt
     1381 tgttgttgac aggaaggttt cttagcgctt tttttgattt tttgacgtta ttgtgcatct
     1441 gccgattcag ccggaatcta tggaaaagtc gtaaggcggc tttcttaagc gtttgcgggt
     1501 acggtttttt tgtcggagcg atccaacagg cacatttttt tacggttgat tctttttgca
     1561 ctttcttttg cactttctct cttctttctg ttttgggtga tcgaaagaag catttctatt
     1621 cgcaagccat tgtcagcgga attgcattgg ggatcgcgct ggcttgtaaa atatcagcga
     1681 ttttatttat tttgtttccc gcgcttgata tggtcttccg gacggttcgg aggcatagcg
     1741 atatcaaaaa agaattgttt cgattctttt tgatgatttc agcttccgct tttactttca
     1801 ggatttttca gccttatgct tttcagaatc cgcaattctt cgattttcgg ttaaattcat
     1861 cgtggatcgc cgcaatgcgg gaattggctg aacaaagcag cggcagagtc gattttccgc
     1921 ctgcatggca atgggtggga agatcaccgt tttatgccct gaagaatctg ttgatttatg
     1981 ggatgggatt attaccaggg ggattggttt tcgccggctt tgttctgtct atccgtcagg
     2041 gattcaaaaa agacaattac gtcatattga cgctcatcat cggaacagcg gtttttttct
     2101 tggcgcaaac cgctcaattt tcaaagatga tgcgttatct ttaccccata tgtccgattt
     2161 tgtttctgct tctcggatct cttctcgaag ttgaagggaa agtctggatg aagattttcc
     2221 tatcgacagc tctcgcatgc gcttttctcc gatcatgtgc cttcctttca atttatcagc
     2281 gtccccatac ccgtgttcag gcaagccggt gggtctatga tcaaattccg gctgcgatat
     2341 ccgttcaagt ctcgggagag acggatgaag ttgaaccgga tttgcaagtt gcggtaccga
     2401 cgattttttc tgttgaaaca ggggatgacg caatccaaat tccattggcg gttcaaaaag
     2461 gggacattcc tcgattaata gagttcgaaa atttaaaagt aaacggtgaa aattgttcga
     2521 tccgagcttc gatcaacagt acggaagaaa atcgggactt attttgacgg gtgatttgat
     2581 cccgaatgaa tcacaaatcc tatttgccgg ggatgacgcg attctcggca atcaatcttt
     2641 tctttcattg caaacgatag ggaacggctg ttctgccgag ataagcgggg attcttttct
     2701 ttattttctt cggaatggca aaccccgtta tttgttattg aaagggctgg ctgctgcgtg
     2761 gataaccgat gacaaaccgt tggaagttca ttttaagata caagacgacg gttcaattgc
     2821 cggtttgagc tttcattcga tgatgttcag tggcggaatg gatcaatccg tgccgatttc
     2881 gttcactttt ataaattaca acaattccac cgaaaaaacg gaaaactttt caatcattga
     2941 ttccacgaat caaccggttg ctttttcact ttccgatccg atcaaagtaa aagcaggtga
     3001 gatgattgga ttgcgtatag ccgtaaattc agaacgagct atatccgttc aaccgaaacg
     3061 aattgcaacc gaaagcagct gggatga
//
LOCUS       DAOMIV010000235         3074 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_34050_length_3074_cov_3.346472, whole genome shotgun sequence.
ACCESSION   DAOMIV010000235 DAOMIV010000000
VERSION     DAOMIV010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34050_length_3074_cov_3.346472"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..607
                     /locus_tag="PKV59_06200"
     CDS             53..607
                     /locus_tag="PKV59_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQP46380.1"
                     /translation="MQDRSRKIAVTGVLGAVTILLGLTHWGFIPWFGGVSLTIMHIPV
                     IIGAILEGPAVGAGIGLIFGLFSMLQAAIAPSGPTDVWFTNPLLSILPRLMIGPITWL
                     VYHSLKHWQAGAIIISAIAGSATNTVLVLGMMGLLRLLPWQVLGGIALINGLPEIGAS
                     ALISFLVIVAVRQIKVGKRQGSEV"
     gene            complement(620..1438)
                     /locus_tag="PKV59_06205"
     CDS             complement(620..1438)
                     /locus_tag="PKV59_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQP46381.1"
                     /translation="MNDFDFLSKTTIGQYIPTGSILHHLEPGSKLIGFGILILAMTFS
                     PSIIGVGIGLIASLIGLIIAKIPLRFALKGILIPLPFLIVIALIQIAFFPIRGQSVLF
                     RVGPIQVSILSLMSGLLLIFRFSALILAISLMTFCVSASEMIHGLQHILHPLNALGIH
                     TMDFVMMIQIALHFLPLLAQSAERIAKAQASRGAEWGIKSKGLLSRVKQIFPLLIPLF
                     TISLTRAETMALAMDARAYGFKANRTSMVEYSFGRREAVFLLIVLWIVVLIFIF"
     gene            complement(1431..>3074)
                     /locus_tag="PKV59_06210"
     CDS             complement(1431..>3074)
                     /locus_tag="PKV59_06210"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HQP46382.1"
                     /translation="GELIALVGANGSGKTTLARQLNALLLPTEGNVYINGENTRNRQA
                     VPKIRSEIGMVFQHPEDQIVATIVEEDVAFGPENLCRLPKIIREQVNRSLAVVNAESY
                     RYRQPHMLSAGQMQRVALASVLAMQPSAIIFDETTAMLDPIGRRDVLQCMVDLNRMGI
                     TIIFITHIMEEVNLANRVLLLNEGSLIFDGAPEVLFSDPNLLDAGGLEQPTEYRIYEA
                     FPDWFTGFKGKKIDLKDLFGAIPDFTGPFRQRDKLTSRSSQPAIDEIEIQNLSYTYLA
                     GNILQQQALYDVSMTVPKGFPHGIIGATGSGKSSLLQHLNGLYLPQSGRVKVGPFDLN
                     SMNFDLRALRHYAGLVFQNPESYFFKQYVGDEIAYGPKLFYGTENLRERVKWAMNFVG
                     LDFETFKDRITATLSGGEQRKVALAATLASHPSLLIMDEPTCGLDPRSRRNLIENLRS
                     LQKNEMQLIISTHNMDDIVELAENVTVLSEGRSISNLPVSQVFSDREKLESVGLDQPI
                     SVRFAQCLRDKGWPISSDAVTLGEIFTEVNDLLSFNKNE"
BASE COUNT          890 a          709 c          656 g          819 t
ORIGIN      
        1 tttacgaata atcatcaata tatctcgttt gattatatcg gaggctcaaa aaatgcaaga
       61 tcgatcgcgt aaaattgccg tgacaggcgt actgggtgcc gttacgattc ttttggggtt
      121 aactcattgg ggatttattc cttggttcgg cggagtatcc ctgaccatta tgcacattcc
      181 ggtcatcatc ggtgccattc tcgaaggacc ggctgtcggc gcaggcatcg gtttaatttt
      241 tggattattc agtatgcttc aagctgccat agccccttcc ggacctacgg atgtttggtt
      301 caccaatcct cttctttcga tacttcccag attaatgatc ggaccgatca cgtggctggt
      361 ttatcattca ctgaaacatt ggcaagccgg tgcaatcatc atcagtgcca tcgccggatc
      421 ggcaaccaat acggttctgg ttttagggat gatgggattg ttaaggctgc tcccatggca
      481 ggtgttgggt ggaatcgcac tgattaatgg cttgcccgaa atcggcgcat ccgcactgat
      541 ttcattctta gtaatcgtcg cagtccgaca aatcaaagtc ggcaaaagac aaggttccga
      601 agtgtaaatt ctccgccaat cagaaaataa aaatcaaaac gacaatccat aagacaatca
      661 aaagaaaaac ggcttcacgc cgcccaaaag aatattccac catgctggtg cgatttgctt
      721 taaatccata tgctctggca tccattgcaa gtgccatcgt ttccgctcgg gttaaactga
      781 ttgtaaaaag cggtatcaac agtggaaaga tttgcttcac acggctcaag agtcccttgg
      841 atttaattcc ccattccgct ccgcgtgaag cttgagcttt ggcaattctc tccgcagatt
      901 gagctaaaag gggtagaaag tgcaacgcga tttggatcat catgacaaaa tccatcgtat
      961 gaatccccaa tgcgttcagc ggatgaagga tgtgttgtaa tccatgaatc atttccgaag
     1021 cagatacaca aaaagtcatc agactgattg ccaaaatcaa tgcactgaat cgaaaaatca
     1081 acagcaatcc cgacattaag ctgagaatcg aaacttgaat cggcccgact cgaaacaaca
     1141 cggactgacc tcgaatcggg aaaaaagcaa tttgaatcaa cgcaataacg atcaaaaaag
     1201 gcagagggat caatattcct tttagagcaa aacgcaaagg aattttggcg attattaatc
     1261 cgataagaga agcgattaat ccgataccga caccgattat cgaaggactg aatgtcatcg
     1321 ctaaaatcaa gatcccgaat ccgatcagct tcgaaccggg ctcaaggtga tgaaggatag
     1381 atccggtcgg aatgtattgt ccgatcgtgg tttttgagag aaaatcaaag tcattcattt
     1441 ttattaaaac tcagtaaatc gttcacttcg gtaaaaattt cacctaatgt tacagcgtca
     1501 gatgaaatcg gccaaccttt atcccgtaaa cattgtgcaa aacggacgga aatcggttga
     1561 tccaatccta ccgactccaa tttctctcga tcggaaaaca cttgggaaac cggtaaattg
     1621 gatatgcttc gaccttccga taggacagtc acattttctg ccaattcgac gatatcgtcc
     1681 atattgtgag ttgaaataat taactgcatc tcattttttt gcagtgatcg cagattctct
     1741 atcaaatttc gccgggatcg cggatcaagt ccgcatgtcg gctcatccat aatcaaaagc
     1801 gaaggatgag aggccagagt agccgccaaa gccactttcc gctgttcgcc tccgcttaaa
     1861 gtggcggtaa ttcgatcttt aaaggtttca aaatcgagcc ccacaaaatt catagcccat
     1921 ttcacgcgtt cccgtaaatt ttccgtcccg taaaataatt tcggaccgta agcaatttcg
     1981 tctcctacat attgtttgaa aaaataggat tccggatttt ggaagaccaa tccggcgtaa
     2041 tgtcgcaatg cccgcagatc gaaattcatg gaattcagat caaacggacc gacttttaca
     2101 cgaccgcttt gcggaagata cagtccgttc aaatgttgca aaagactcga ttttcctgat
     2161 ccggtcgccc cgataatacc atgtggaaat cctttgggaa cagtcatcga aacgtcgtaa
     2221 agagcctgct gctgtaaaat atttccggct aagtatgtat aagaaagatt ttgaatttca
     2281 atttcatcga tggccggttg cgaagaccgc gaagtgagtt tgtctctttg cctaaaaggg
     2341 ccggtgaagt ccggaatggc tccaaacaga tcctttagat caattttttt gcccttaaaa
     2401 ccggtaaacc aatcaggaaa agcttcgtaa atacgatatt cagtcggttg ttcgagccca
     2461 ccggcatcca acaggttcgg atcggaaaaa aggacttcgg gcgctccgtc aaaaatcaga
     2521 gaaccttcat ttagaagaag aactcgattt gcgagattta cttcttccat aatatgtgtg
     2581 ataaaaataa tcgtaattcc catccggttc aaatcgacca tacactgaag tacatcccgg
     2641 cgaccgatcg gatccaacat agcggttgtt tcatcgaaaa tgatcgcgct cggttgcatg
     2701 gctaacacac tggccaatgc cacccgttgc atttgtccgg ctgaaagcat atgaggttgt
     2761 ctgtaacgat agctttctgc attgacaacg gcaagggatc tattcacttg ttcgcgaatt
     2821 atttttggaa gacggcataa gttttctggt ccgaaagcga catcttcttc aacaatcgtc
     2881 gcaacaattt ggtcttcggg gtgttgaaaa accatcccga tttcagatcg tattttcgga
     2941 acagcctgcc gattacgtgt attttccccg ttaatataca cattaccttc ggtcggtaaa
     3001 agcaaagcat ttaactgacg ggcaagagtc gttttaccgg atccgtttgc acccaccagt
     3061 gcaatcaatt ctcc
//
LOCUS       DAOMIV010000236         3072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_34077_length_3072_cov_4.404707, whole genome shotgun sequence.
ACCESSION   DAOMIV010000236 DAOMIV010000000
VERSION     DAOMIV010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34077_length_3072_cov_4.404707"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="PKV59_06215"
     CDS             <1..665
                     /locus_tag="PKV59_06215"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HQP46383.1"
                     /translation="PVVALTDGPLQLYYFQEPNTTENPLVNEYYKALAEMSEKKIITA
                     GYTDRPRSTFVIDMLRLLMNSDPQDLRVKQTLVFDSNLFSRILLPGERSALFKLISPA
                     VEQMPAVVQISFFYLNVGNDSRPMITRIEIPEWVSKDPAAIDLLHYSLLQQNGFISSN
                     PYPYALHRAHECAVVQFSEKEHVKQLIIRELLSRGVTDLEKSAKQIAKDLFGTTYRDF
                     EE"
     gene            704..1345
                     /locus_tag="PKV59_06220"
     CDS             704..1345
                     /locus_tag="PKV59_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46384.1"
                     /translation="MTDTSMFGRILSSNIRECVVGYRTSESAIPPFGSMVRIPVSHAQ
                     QLEIYGLVTDIQIEEDGFLRQLATNDQVSEEVMLDARANRNVPVVLRILFIGYLENGN
                     ISHLLPPCPPLTLDNIYKCTNAEITAFTSFGNFGYIRHILKVTNVPPGELLAAHISQA
                     AKAQFADNHFGWTERAINEVIYLMRDNYADLLAILGAIGDADISLDGEEEEQE"
     gene            1342..3000
                     /locus_tag="PKV59_06225"
     CDS             1342..3000
                     /locus_tag="PKV59_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP46385.1"
                     /translation="MTPAENIGFVIGGSLKEGLFVRLTVPAERIQEGAFVVIESNGLK
                     FYGLVVNLSLKSYDPRYSQDLIAGRMSDKITTLLHNQTLFTEMEVLPVLMQNTGPELE
                     DPNYDPNAVMDKSPMPVKTLPIHQAPVRLATEFDVSEVFKPKEKNGFVIGTTREQGHP
                     VHIDMDKFIQRSSGIFGATGTGKSYLTRMILAGLIQSKQAAVLVFDMHNEYAYGDISP
                     DTDIKAPGLCEKMKKRVKSCALGASAKIAGNQPDFDLVLEYSDILPSDIEMLTRELNL
                     RETTPATLSALVQSFGINKWFESFKNLTMGEGEGSVVDWARNNNVNEAAALSLYSKLE
                     RVFRLNYLVPKLTGPSSVVKIIDYLKNGYSVILSFGEYETDLDYLLVTNILTRKIREE
                     WERMTNEYRNQGGVKPTPLVIALEEAHKLLNREMAGQTTFSTIAREMRKYFVTLLIID
                     QRPSQIYDEVMSQLGTRISGWLGDDEDIAAVLSGLSGRDSLRGMLARLQPKEEVLLLG
                     YGVPMPIPIRSRRYDAQFWQEILSEDELSETDRQKFREMVFDDF"
BASE COUNT          865 a          618 c          746 g          843 t
ORIGIN      
        1 aaccggtcgt tgcactgaca gacggccctc ttcagctata ttatttccaa gagccgaaca
       61 ctacggaaaa tcccttggtc aatgaatact acaaagcatt ggcggagatg agtgagaaaa
      121 agattatcac ggcagggtat acggatcgtc ctcgctccac ctttgttatc gatatgctgc
      181 ggttgctgat gaattcggat ccgcaggatt tgagagttaa acagacgttg gtttttgaca
      241 gcaacctgtt ttcacgcatt cttttgcccg gtgagcgctc ggcacttttt aaactgattt
      301 cgccggctgt tgaacaaatg ccggcggtgg ttcagatatc ctttttttat ttgaatgtgg
      361 ggaatgattc acgcccgatg attacccgta ttgaaattcc cgaatgggta tcaaaagatc
      421 cggctgcgat tgatttgctt cattattcgc ttttgcaaca aaacggattc atttcgtcta
      481 atccatatcc ctatgctcta catcgcgctc acgaatgtgc tgttgtgcaa ttcagtgaaa
      541 aggaacatgt taaacaactg atcatccggg aattgctttc gcgcggtgta accgatttgg
      601 aaaaatccgc aaagcagatt gcaaaggatt tattcggaac aacctaccgc gattttgagg
      661 aataaaacag gaaaatcgga tctttataca gaagaggggt ctcatgacag atacaagcat
      721 gtttggaaga attctcagtt cgaacattcg ggagtgcgtc gtcggttatc ggacaagcga
      781 atccgccatt ccaccttttg gttccatggt ccgtattccg gtaagtcatg ctcagcaact
      841 ggaaatctat ggattggtga cggatatcca gattgaagaa gacggattct tgcgtcaact
      901 ggctaccaat gatcaggttt cggaggaggt aatgttggat gcccgggcaa atcgcaatgt
      961 gccggttgtc ttaaggattt tgtttatcgg ctatctggaa aacggaaata tatcccattt
     1021 attaccgccc tgcccgccgt tgacgttgga taatatttat aaatgcacga atgccgagat
     1081 cacggcattt acctctttcg gaaattttgg atacattcga cacattctga aggtaacgaa
     1141 tgttccgccg ggtgaattgc ttgcggcaca tatcagtcag gctgctaagg ctcagtttgc
     1201 cgataatcat ttcggttgga cggaaagagc cattaatgaa gtgatctatt taatgcgaga
     1261 taactatgcc gatctgttag cgatattggg agcgatcggc gatgcggata tcagtttgga
     1321 tggagaagag gaggaacagg aatgaccccg gcagaaaata tcggatttgt aattgggggc
     1381 agtttaaaag aaggtctgtt tgttcgtcta accgttcccg ccgaacgaat tcaggaaggc
     1441 gcttttgtcg tcattgaaag taacggattg aaattttacg gattggttgt taatctcagc
     1501 ctcaaatctt atgatcccag atattcgcaa gatttaatcg ccggcagaat gtccgataaa
     1561 attacgactt tattgcataa ccagaccctt tttaccgaga tggaagtttt accggttctg
     1621 atgcaaaata cgggtccgga actcgaagat ccgaattatg atcccaatgc cgtgatggat
     1681 aaatcaccca tgccggttaa aactctcccc attcatcagg cacctgttcg gcttgcaacc
     1741 gaattcgacg tcagcgaagt gtttaaaccc aaagaaaaaa atggattcgt catcggtacc
     1801 acgcgcgagc aaggacatcc ggttcatatt gatatggata aatttattca gcgctcttcg
     1861 ggaatttttg gggcgaccgg aacggggaag agttatttga cccgcatgat cttagccggg
     1921 ttaattcaaa gcaaacaagc tgctgtgctg gtttttgata tgcacaatga atatgcatac
     1981 ggcgatattt cacccgatac ggatataaaa gcgccgggtt tgtgcgaaaa gatgaaaaaa
     2041 agagtcaagt cttgtgcttt gggagcttca gcaaaaattg caggcaatca acccgatttt
     2101 gatttggtac tggaatattc ggatattcta ccgtccgata ttgagatgct gacccgggaa
     2161 ttgaatctgc gcgaaacgac tccggctacg ctgtcggcgt tggttcaatc cttcggtata
     2221 aataaatggt tcgagtcatt caaaaatcta acaatgggtg agggagaggg ttcggtcgtg
     2281 gactgggcgc ggaacaacaa tgtgaatgaa gctgccgcac tgagtctgta tagcaaactt
     2341 gaacgggttt ttcggttgaa ctatcttgtt ccgaagttaa ccggtccttc ttcggttgta
     2401 aaaataatcg attatttaaa aaatgggtat tcggttattt tgtccttcgg agaatatgag
     2461 acagatttgg attatttgtt ggtaacaaac attctgacac gaaaaattcg tgaagaatgg
     2521 gaacgaatga cgaatgaata tcgaaatcaa ggcggtgtca aacctactcc gctggtgatt
     2581 gcattggaag aagcacataa attattaaac cgggaaatgg ccggacaaac aacattttca
     2641 acaattgcca gagaaatgag aaaatatttt gtgactctac tcattatcga ccagcgtcca
     2701 tctcaaattt atgacgaagt gatgtcccaa ctgggaactc gtatctcagg ctggctggga
     2761 gatgatgagg atatcgcagc agtactatcc ggactttccg ggcgggattc attacgggga
     2821 atgttagccc gtttgcaacc gaaggaggaa gttcttcttc tcggatacgg cgttcctatg
     2881 cctatcccta ttcgttcccg tcgatatgat gcccaattct ggcaggaaat actgagtgag
     2941 gatgaacttt ccgagacgga tagacaaaaa tttcgggaaa tggtttttga cgatttttag
     3001 gaatctttca aaaaccaatg tgagaatttc atgacagagg ccggtccaaa ccgatcgatc
     3061 gaaagacaaa aa
//
LOCUS       DAOMIV010000237         3064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_34197_length_3064_cov_4.145231, whole genome shotgun sequence.
ACCESSION   DAOMIV010000237 DAOMIV010000000
VERSION     DAOMIV010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34197_length_3064_cov_4.145231"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..770
                     /locus_tag="PKV59_06230"
     CDS             72..770
                     /locus_tag="PKV59_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQP46386.1"
                     /translation="MEITWYGHSCFRITERGMGTVVCDPYDSQEAGFEPLKLKADVVT
                     CSSNHPGHHFISAVKGDPFIIDSPGEYEINNVFINGYRSGETDGVKNTIFVIEYNGLF
                     VAHLGNLNRIPSLSEIETYGTIHVLLIPVGGGGALNSSKAVELISQIKPNIVIPMHYA
                     DPLGKQELDPLSKFLKEMGISQTEDVYNSLKITNATQLPEETKLIILNHPLSDSLNDQ
                     DDSAEDNNEEVNAG"
     gene            760..1062
                     /locus_tag="PKV59_06235"
     CDS             760..1062
                     /locus_tag="PKV59_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46387.1"
                     /translation="MPVKLIMTWDILPNHEQEYFDFLVREFVPSLNRLGFELSDAWAT
                     IYGDQPQVQISALLPNESEAEQRMAKPEWMELKEKLQQFIQNFECKLVPARQGFQF"
     gene            1323..2429
                     /gene="mutY"
                     /locus_tag="PKV59_06240"
     CDS             1323..2429
                     /gene="mutY"
                     /locus_tag="PKV59_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HQP46388.1"
                     /translation="MNKPMHQELLEWFGKNKRDLPWRENRTPYRVWISEIMLQQTRVE
                     TVIPYYTRWMEAFPDPVSLAKSDEQQVLKQWEGLGYYSRARTLYKTAGIIMQKWNGIF
                     PNDLKTLESLPGIGRYTAGAIASIAFNQAVPALDGNIRRIYSRLFDLAVPLRTKESET
                     ILWQLAGSEIEGENSPGAYHEALMDLGSSVCLPSAPRCLLCPLTSWCEAFKKNTVSQR
                     PVMKQTKSIPHHTVTAGIIRKPNDEVLIAQRPAHGLLGGLWEFPGGKQEKDETLEECL
                     IRELWEELEVQVIIKAPFGVYKHAYTHFRVTLHAFLCEIGDQNPQPIEAQALRWVEIP
                     NLAAFPMGKIDRQIARDLQKLEPIRNEEESIDNL"
     gene            2426..>3064
                     /locus_tag="PKV59_06245"
     CDS             2426..>3064
                     /locus_tag="PKV59_06245"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQP46389.1"
                     /translation="MNYLENLNPQQYQAVTAGEGSVLVLAGPGSGKTRVLTQRIAYLI
                     KEMGVPAYNILAVTFTNKASREMQDRVEDLLGESIQGNMWLGTFHAVCARILRREASL
                     LPFGHNYVIMDADDQVTLIKQIIKELNLDDKIFRPNSVHNEISNAKNNLLFPQDMLRS
                     NYREETVFRVYERYQQALEKSNAVDFDDLLLWCWKLLKDNPDIRDRYSRRFRH"
BASE COUNT          894 a          660 c          708 g          802 t
ORIGIN      
        1 tcattttcat tcctcgacac agggtataat atatctgtta agattaaatc tagattcatt
       61 caggagaatt catggaaatc acttggtacg gtcattcctg ttttcgaatc acagaacgag
      121 ggatgggcac tgttgtttgt gacccctacg attcacagga agccggtttt gaacccttaa
      181 aattaaaagc cgacgtggta acctgcagca gtaatcatcc ggggcaccat tttatttcgg
      241 ctgtgaaagg ggatccattt atcatcgaca gccccggaga atatgaaatc aataatgttt
      301 ttattaatgg ttatcgatcc ggcgagaccg acggagttaa aaataccatt ttcgtcattg
      361 aatataacgg tttattcgtc gctcatttgg gcaatctgaa ccgaataccg tctctgtctg
      421 agattgagac atacggtacg atccatgtgc tgcttattcc ggtcggaggc ggcggtgcgc
      481 tcaattcttc taaagccgtt gaattgatca gccaaattaa acctaatatt gtgattccga
      541 tgcattatgc cgatccttta gggaaacagg aactggatcc gttatctaaa tttctcaaag
      601 aaatggggat ttcacaaacg gaggatgttt ataattcgtt aaaaatcaca aacgccactc
      661 agcttccgga agagaccaaa ctgatcattt tgaatcatcc tctcagcgat tcattgaatg
      721 atcaggatga ttccgcagaa gacaacaacg aggaagtaaa tgccggttaa attaatcatg
      781 acctgggata ttctgccaaa tcatgagcag gagtattttg attttctggt tcgggaattt
      841 gttcccagct taaaccgctt aggttttgaa ctttcggatg cttgggcaac catttacgga
      901 gatcagccac aggtacagat cagcgctctg ttacccaatg aatcggaggc cgaacagcgg
      961 atggcaaaac cggaatggat ggagctgaaa gaaaaattgc agcaatttat tcaaaatttt
     1021 gaatgtaaat tggtccccgc caggcaaggt tttcagttct gatctcaaaa agcttttcaa
     1081 tgatcaaatg cgggggcttg ctcccgcatt tttgttcgga aattgaatca ttcactcgat
     1141 cgagattaat aaattgctca gagaataaaa tcacccttat taattaaagg ttaaagcctc
     1201 gactcataaa agcagatcaa tcaatatttc ggcaaaatct aaccggcctg tctcgatgaa
     1261 gaaaagaatt ctatttacag aaccgattat caatttggga caaatatatc gaagaaacaa
     1321 taatgaacaa accgatgcat caggaacttt tagaatggtt cgggaaaaat aaaagagatc
     1381 tcccgtggcg ggagaatcgc acaccttacc gggtgtggat ttcagaaatc atgttacaac
     1441 aaacccgcgt cgagacggtg attccctatt atacccgttg gatggaagcc tttcccgacc
     1501 cggtgtcgct tgcgaaatcg gacgaacagc aggttctgaa acaatgggaa gggttgggat
     1561 attacagtcg agctcgaacg ctgtacaaaa ctgccgggat aatcatgcaa aaatggaatg
     1621 gaattttccc gaatgactta aagacgcttg aaagtctccc cggaatcgga cgatatactg
     1681 ccggtgcgat cgcatccatt gctttcaatc aagcggttcc cgcattggat gggaacattc
     1741 gccggattta ctcccgtttg tttgaccttg ccgttcccct tcggacaaag gagtcggaaa
     1801 cgattctttg gcagctcgcc ggttcggaaa ttgaaggaga gaattccccc ggtgcttacc
     1861 acgaagcatt gatggatttg ggttcttccg tctgcctgcc gtccgcaccc cgatgtttgc
     1921 tctgcccctt gacaagttgg tgcgaagctt tcaaaaagaa tacggtttcg caaagaccgg
     1981 tgatgaaaca gacgaaatcc attccccatc ataccgttac cgccggcatc attcgaaaac
     2041 cgaacgatga agtattgatc gctcaaagac ccgcacacgg attattgggc ggtctttggg
     2101 aattcccggg tggaaagcag gaaaaggatg aaacgctcga agaatgttta atccgtgaac
     2161 tttgggaaga gctggaagtg caagttataa taaaagcgcc ctttggggta tataaacatg
     2221 cctatactca ttttcgggtc actctccatg cttttttatg cgaaatcgga gatcaaaatc
     2281 cgcaacccat tgaagctcaa gccctgcgat gggtagagat tccgaatctg gctgctttcc
     2341 cgatggggaa aattgaccgg caaatcgctc gggatttaca gaaactcgaa ccgatccgaa
     2401 atgaggaaga atcaatcgat aacctatgaa ttatttagaa aacttaaatc cacagcaata
     2461 tcaggcggtt accgccggag agggaagtgt tttggtactg gccggtcccg gttcggggaa
     2521 gacccgagtg ctgacacaac gaatcgccta cctgatcaag gaaatgggag tcccggctta
     2581 caatatattg gcggttactt ttacaaataa agcgtcacgc gagatgcagg atcgtgtcga
     2641 agatcttctg ggcgagagca tacagggcaa tatgtggctc ggtacctttc acgcagtttg
     2701 cgcgcgaatc ttacgacgtg aagcttctct gctccccttc ggtcacaatt atgtcattat
     2761 ggacgcagat gatcaggtga cattaatcaa acagatcatt aaggaactga atctggatga
     2821 taagatcttt cgtccgaatt ccgtccataa cgagatttcc aatgcaaaga ataatctttt
     2881 gtttcctcaa gatatgctgc gcagcaacta tcgggaagaa actgtcttca gagtttacga
     2941 aagatatcaa caagcactcg aaaaaagcaa tgcggtggat tttgatgatt tgcttttatg
     3001 gtgttggaaa ctgttgaaag ataacccgga tattcgggac cgctattccc gcagattccg
     3061 ccat
//
LOCUS       DAOMIV010000238         3047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_34470_length_3047_cov_3.594920, whole genome shotgun sequence.
ACCESSION   DAOMIV010000238 DAOMIV010000000
VERSION     DAOMIV010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34470_length_3047_cov_3.594920"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1634)
                     /locus_tag="PKV59_06250"
     CDS             complement(114..1634)
                     /locus_tag="PKV59_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46390.1"
                     /translation="MKKQTLSILLALVLLLSISIFPVFAQELSEDEVSGYFGAIEDWT
                     TQDLALAGVDSEAVDLTEFEAKSVENPVPTAELTDEVTVEGEKGVDLQAKSIIDPATV
                     TLSGEFVACGDGKIKIEIGLKRTADSSSTGMPNMIFVKSVIVTIGTTDYELQNCKSQY
                     GDACSKVYLDASGNATLTGYVEIPRVLDTTPVSFDVEINYSKTAHGALETPVTKSGGS
                     ATSTKDLCATEGVDVVATKGIYEQCGGKASFKADIEVPAEKGPECLVPAQIYVEGTSY
                     SNYTCRYTRFGSEGYPFGGDYCTPGKAIPVKPGDKIRFEAIINDLTNPVAETSGGADL
                     NVDWRFGYNPAMISGVMESVPGPAELCDAKIVPLKPLDSMEDANDPIEPSGWYNTYDK
                     AVVGLYQKCGKFAVMQIRLRNDGAKTGFINLPGSVAVNGGAPIAFYWLSSVAPHGNKV
                     ALGPGEIVTLVGRIWLTNLISQTNSDGAANVAVNFSDLGLYMTGSLFSDRVNWRCY"
     gene            complement(1941..>3047)
                     /locus_tag="PKV59_06255"
     CDS             complement(1941..>3047)
                     /locus_tag="PKV59_06255"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQP46391.1"
                     /translation="DRDHKALLEGLLLGLFSFDKYKKKEDPVNSVIYLDPSEEILLQT
                     CKTICDAVNMAREWAHEPAAVINPQTLSERAQKFAKQFGLNCKILDDKQLIEMGAGAI
                     VAVGQGSKTPSRMIILEYKGTEKEKPVVLVGKALTFDSGGYSIKPGDSMVGMKYDKCG
                     AMAVLGVLRAASQLKIATPITGIICAAENMLSQNSYRPDDILISLSGKSIEVISTDAE
                     GRLVLADGLTYAQQNFSPQAIIDLATLTGGCAVAYGHVRAGVLTNNQSLYRELFESGE
                     RTWERIWQLPNDEDYAKLIKGKDADIKNSGGRLASPVTGGMFLKEFVSDEVPWAHLDI
                     AGVADLDEGTDYSVPGATGFGVRLLIDWLSNRNQ"
BASE COUNT          779 a          763 c          653 g          852 t
ORIGIN      
        1 tgtttccaga taagggttca ttctctgcaa tactccggaa actcgagaca ttaaaaaatt
       61 tccggatggg aattcccatc cggaaaatga tgattgatct tgacggaccg tgatcagtaa
      121 cagcgccaat tcactctgtc cgagaataaa ctgccggtca tatataatcc cagatccgag
      181 aaattcaccg ccacattggc tgctccgtca ctgttggtct gactgatcag gttggtgagc
      241 cagattctgc cgaccaaagt tacgatttca cccgggccga gagcgacctt atttccgtgt
      301 ggagcgacgg aagagagcca gtaaaaggcg atcggagcac caccgttgac ggctacggaa
      361 cccggcaggt taataaagcc ggtctttgcc ccgtcattgc gcagtcgaat ttgcatgacc
      421 gcaaatttcc cgcatttttg atataagccg acgaccgctt tatcataagt gttataccaa
      481 ccggaaggtt cgatcggatc attcgcatct tccatcgaat ccaacggttt caacgggaca
      541 atcttcgcat cgcataattc ggcgggaccc ggaacgcttt ccataactcc gctgatcata
      601 gccgggttat aaccgaagcg ccaatccaca ttcaaatcgg caccgccgga agtctcggca
      661 accggattgg tcagatcgtt gatgatcgct tcgaaacgga tcttgtcccc tggttttacc
      721 ggaatggctt tgcccggtgt acagtaatca ccgccgaaag gataaccttc ggaaccgaag
      781 cgcgtataac ggcaggtgta attcgagtag gaagtccctt cgacatagat ttgtgcggga
      841 acaaggcatt cgggaccttt ctctgccggc acttcgatat cggctttgaa agaggctttc
      901 ccgccgcatt gttcataaat gcctttggtc gcaaccacgt ccacgccttc tgtcgcgcat
      961 aaatcttttg ttgatgtagc gcttcctccc gatttggtaa ctggagtttc caatgctccg
     1021 tgagcagttt ttgaatagtt aatctcgaca tcaaaagaaa ccggagtggt atctaaaact
     1081 cttggaattt caacatagcc agttaaagtt gcgttcccgc tcgcatccaa atagactttg
     1141 ctgcaggcat ccccatattg agatttacag ttctgtaatt cgtaatcggt ggtaccaatc
     1201 gtcacaataa ccgatttcac aaaaatcata ttgggcattc cggtcgagct gctgtcagcg
     1261 gttcgtttta gaccgatttc aattttgatt tttccgtctc cgcaagcgac aaattcaccg
     1321 gaaagtgtga ctgttgccgg atcaatgatc gattttgcct gtaaatcgac gcctttttcg
     1381 ccttcaacag ttacttcgtc cgttaattca gccgtcggaa ccggattctc aacacttttg
     1441 gcttcgaatt cagtcaaatc gactgcttcc gaatccacac cggctaaagc gagatcttgg
     1501 gtcgtccagt cttcaattgc gccaaaataa ccggagactt catcctcgct taattcctgt
     1561 gcaaatacgg ggaatatgga tatgctcagg agtaatacca gagccaataa gatggaaagt
     1621 gtttgttttt tcattagatt atgacctcat ttctttcgaa ataataattt cttatttccg
     1681 tcccccaata atttgggttt gatttctatg ttgcaatatc aatgccattt atcgattttc
     1741 ctttattttc ccgaaaactt ttcgggaaat atttaaccga tgtcttctat gcgtgcagga
     1801 atcgcgccaa aatgaaggat tttgaaaaat gtctgaaaat ctcttcagag agggacaatt
     1861 gattcgatca actgagtaaa aaaagaagaa gcggggaatc aattcacccg cctcctcttt
     1921 tttaccgcta aatgattttt ttactgattt cgattcgata accaatcgat caacagacga
     1981 accccaaagc cggtggcacc ggggacggaa taatcggttc cttcatcaag gtcagcgacg
     2041 ccggctatat ccaaatgcgc ccatgggacc tcatcggaaa caaattcttt caggaacatt
     2101 cccccggtta cgggagaagc cagacgaccg cccgagtttt taatatccgc atcttttcct
     2161 ttaatcaact tggcataatc ttcatcattg ggcagttgcc aaatgcgttc ccatgtcctt
     2221 tcccccgatt caaacaattc tcgataaaga gattgattat ttgtgagcac tccggctcgt
     2281 acatgcccgt aagcgacggc acatccgccg gtaagggttg ccaggtcgat gatggcttgc
     2341 gggctgaaat tttgttgggc atatgttaat ccgtctgcca ataccaaccg cccttccgcg
     2401 tcggtcgaga tcacctcaat acttttaccg gagagactga tcagaatatc atccggacga
     2461 taagaatttt ggctcagcat attctctgcg gcgcagatga ttccggtaat cggggtcgcg
     2521 attttcagct gagaagcggc tctcaaaact cccaggacag ccatcgctcc gcacttatca
     2581 tatttcatcc cgaccatgct gtcgcctggt ttaatcgaat agccgcccga atcaaatgta
     2641 agcgctttgc cgactaatac gaccggtttt tctttttccg ttcctttata ttcgagaatg
     2701 atcatgcggg aaggcgtttt acttccctga ccgaccgcta cgatggcgcc ggctcccatt
     2761 tcaattaatt gcttatcatc caaaattttg caattcaagc cgaattgttt tgcaaatttt
     2821 tgtgctcgtt cggaaagtgt ctgcgggttg ataaccgcag ccggttcatg tgcccattcc
     2881 cgtgccatat tcaccgcatc acaaatcgtc ttacaggttt gcagcaatat ttcttcggaa
     2941 gggtcaagat aaatgacgga attgaccgga tcctcttttt tcttgtactt atcaaaggaa
     3001 aataaaccca acaacaatcc ttccaataaa gctttatgat ctctgtc
//
LOCUS       DAOMIV010000239         3043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_34530_length_3043_cov_3.878849, whole genome shotgun sequence.
ACCESSION   DAOMIV010000239 DAOMIV010000000
VERSION     DAOMIV010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34530_length_3043_cov_3.878849"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..787)
                     /gene="pyrE"
                     /locus_tag="PKV59_06260"
     CDS             complement(125..787)
                     /gene="pyrE"
                     /locus_tag="PKV59_06260"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQP46392.1"
                     /translation="MKREIAQHLLDIKAVFLRPNEPFTWVSGIKSPIYCDNRLTLSYP
                     EIRKDIENGLVSIIREHYPDCEAIFGTSTAGIPHAAIVADIMDLPMGYVRGNTKDHGR
                     NNQIEGRFEKGWKVVVIEDLISTAGSAVEVVQILRENGCNVLGIASIFTYGMQAGLDN
                     LRKAEVQNYSLTDFDTLIEVELENGSLSRADVNKLLAFRANPSDSSWQFCTSAVENPQ
                     IY"
     gene            complement(829..1530)
                     /gene="pyrF"
                     /locus_tag="PKV59_06265"
     CDS             complement(829..1530)
                     /gene="pyrF"
                     /locus_tag="PKV59_06265"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQP46393.1"
                     /translation="MKDVIIACDFKDAKTTLSFLDLFKTKKPFVKIGMELFYFEGPAI
                     VREIKARGHKIFLDLKLHDIPNTVMSAMQSLSKLDPDIIDVHAAGTTEMMKAAMEGAF
                     RSDGSKPLVIAVTQLTSTSEERMNRELLIQATMEETVLHYAQNAKEAGLAGIVCSPLE
                     AAIVKKGLGEDFITVTPGIRLPDSIKDDQARITTPVQAREIGTDYIVVGRPIIRSENP
                     VAAYDRFCHDFLGEA"
     gene            complement(1836..>3043)
                     /locus_tag="PKV59_06270"
     CDS             complement(1836..>3043)
                     /locus_tag="PKV59_06270"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HQP46394.1"
                     /translation="MNILIFNCGSSSQAFKVYQVSEKDQPKIIASGKAKNVATQTKAD
                     SFIEYRINDKQRKEIISLPSHRIAADVILTLLERENITIDGIGHRFVHGGPRFNQSVK
                     IDENVFYELKNSFRFAPIHNPNSFSVIEVCKARLPEIPQYAVFDTAFHAKLPKESQVY
                     ALPKKLAGEYGFRKYGFHGLSYQNILSKMPDLLGKPIEQLNLVMCHLGTGGSSVTAIK
                     NGLPYDTSMGYSPLQGLIMSTRCGDLDPEIVLDLIRSGMSADEVDSVLNNQSGLIGLS
                     GYSSNLIEIIDESERGNEDCQLAYDAYSHRLKSYIGAFVWLLDGADAIVFTDDLGIKS
                     WKLREKVFKDSQNMGIRLDDNANQNASFEKPSLVSRNDSKIPIWVIPNDEEIVILNEI
                     LELIGNGTL"
BASE COUNT          863 a          665 c          580 g          935 t
ORIGIN      
        1 caatgaatat tcattatcag acggcaatgc cgaatttcaa taaacgggtc tgattcatat
       61 gtacgaatca gacccgtaat caaccgattt atcgggaacc gataaataat gaaatcggct
      121 tgaatcaata aatttgcgga ttttcgacag cggaggtgca aaattgccaa gaactgtctg
      181 aaggatttgc ccgaaacgcc aacaatttat tgacatccgc tcttgaaagc gatccgtttt
      241 ccaattccac ttcaatgaga gtatcaaaat cagtcaaaga ataattttga acttccgctt
      301 ttcgaagatt atctaacccc gcttgcattc cgtaagtgaa aatcgaagca attcccagaa
      361 cattacaacc gttttcacgg agaatctgaa caacttcaac ggcgctgccg gctgttgaaa
      421 tcaaatcttc gatgacgacc actttccaac ccttttcaaa acggccttca atttgattat
      481 ttcgtccgtg gtctttagta tttcctctga cataacccat tggaaggtcc ataatatccg
      541 caacgatcgc tgcgtgagga attcctgcgg tagaagtccc aaatattgct tcacaatcag
      601 ggtaatgttc ccgaatgatc gaaaccaagc cgttctcaat atctttacga atttccggat
      661 aagagagcgt aagccgatta tcacaataga tcgggctctt aattccgctg acccaagtaa
      721 acggttcatt ggggcgtaga aaaacagctt tgatatctaa cagatgctga gcgatttccc
      781 ttttcatttc tatcctcctg aatggtgatt caatcatact ccgttgatct aagcttcacc
      841 taaaaaatcg tgacaaaacc gatcataagc cgcgaccgga ttctctgaac ggatgatcgg
      901 acgtcccaca acaatatagt cggtaccgat ttctcttgct tgaacgggag tggtaattct
      961 tgcctgatca tctttgattg aatccggcaa gcgaattccc ggagtgacgg taatgaaatc
     1021 ctcccccaat ccctttttta cgatagcggc ttcaagagga ctgcatacga tgcccgccaa
     1081 cccggcttct tttgcatttt gcgcataatg cagtacggtt tcctccatag tggcttgaat
     1141 cagcaattcc cgattcattc tttcttcgct ggtagaagtg agttgcgtta ccgcgattac
     1201 aagcggtttt gatccgtcgg atcgaaaagc tccttccatt gcggctttca tcatttcggt
     1261 tgttccggct gcgtgaacat cgatgatgtc cgggtccagt tttgaaagac tttgcatggc
     1321 ggacataacg gtattcggaa tatcatggag ttttagatca agaaatattt tatggccgcg
     1381 agctttaatc tcgcgaacga tagccggtcc ttcgaaatag aatagttcca ttccgatttt
     1441 tacaaaaggt ttcttggttt tgaacaaatc caaaaatgac agtgtggttt tcgcatcctt
     1501 aaaatcgcag gcaatgatta cgtctttcat gatatggttc cttttatttg tgagagttga
     1561 attatccata acttttttct tgattaacat aaaaaaaggt ctcgcgcgca gcgagacctt
     1621 caggtgaatt cctattttgc ctattcattc aatcttgccg atctctctgt atcgcgctta
     1681 aagatttttt attgggttta gtttatcatg attttatctt tgataaaaaa gcaatttttc
     1741 gatcgtgaca attttaaagg tatcagaaaa aatgtgtcaa aaagtccgta aatttttctt
     1801 ctaaaacgag atatatttat tcgaattcag ataaatcaca aagtgccatt ccctattaat
     1861 tctaatattt cattcaagat aactatttcc tcatcattcg gaattaccca aatcggaatt
     1921 ttagagtcat ttcggctgac gagcgaaggt ttttcaaaag aggcattttg atttgcatta
     1981 tcatctaatc tgattcccat attttgcgaa tccttaaaaa ccttttctcg caatttccat
     2041 gattttatcc cgagatcgtc cgtaaataca atcgcatccg ctccgtccaa cagccagaca
     2101 aatgcaccga tataactttt taagcgatgg ctgtaagcgt catatgccag ctgacaatct
     2161 tcatttcctc gttcgctttc gtcgattatt tcaatcagat ttgaagaata tccggataat
     2221 ccgatcaatc cggattggtt attcagaaca gaatccactt catccgcgct cattcccgaa
     2281 cgaatcaaat caagcacaat ttccggatcc agatcgccgc aacgagttga catgatcagt
     2341 ccttgcaagg gagaatatcc catcgaagta tcataaggca atccattctt gattgccgtt
     2401 acgctggatc cgccggttcc caaatggcac atgaccagat tcaactgttc gatcggtttt
     2461 ccgagcaaat ccggcatttt cgaaaggata ttttgataag acagaccgtg gaaaccatat
     2521 ttacgaaagc catattcacc ggcaagtttt ttgggaagtg catagacttg ggattctttg
     2581 ggtaattttg catgaaacgc tgtatcaaag acagcatatt gtgggatctc cggcagcctt
     2641 gctttgcaaa cttcgatcac actgaaggaa tttggattgt gaatcggggc aaaacggaag
     2701 gagtttttta attcataaaa aacattttca tcgattttaa cactctgatt gaaacgcggc
     2761 ccgccatgaa caaaacgatg ccctatgcca tcaatcgtga tattctctcg ttccaataaa
     2821 gttaatataa cgtcggcggc aatacgatga gacggaaggc taattatttc tttcctctgt
     2881 ttatcattga tccgatattc gatgaatgaa tccgctttcg tttgcgtagc gacatttttt
     2941 gcttttccgg atgcgattat tttcggttga tctttttccg agacttggta aactttgaat
     3001 gcttgtgatg aactgccaca attaaaaatt aaaatattca taa
//
LOCUS       DAOMIV010000240         3039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_34589_length_3039_cov_4.730563, whole genome shotgun sequence.
ACCESSION   DAOMIV010000240 DAOMIV010000000
VERSION     DAOMIV010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34589_length_3039_cov_4.730563"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..2140)
                     /locus_tag="PKV59_06275"
     CDS             complement(143..2140)
                     /locus_tag="PKV59_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQP46395.1"
                     /translation="MNNERSKIYLTFDQKRVEAKDGMTILQIAQANRIDIPTYCVVKD
                     LTPTGACRVCLVSVTFPNGSQRLVTACDTLAIDGMRVRTDTEEVLNSRRQAAEMLLSI
                     SPDTPSLQRLAATYGISQPSYRTQSLQKGCIQCGACVQACREKSAGVLQFRGNGENRV
                     VTTANGSHASACDSCDICIKYCPTGAVTQSLGLGIGDQVKAKKVGRVSARSRSRLFFL
                     SLFILLILMSGFGIQIQGFPNQLFSLMDPLQGSVVLVSGHPEAINYLPALAILILTIL
                     FGRFWCGWICPTGTVLQSFGPNGRRIKSQRLRNLKQIFFVLILLLAAMGSLAFFWTDP
                     IALLTRPFAALFAYSGEKDFASFDWIGIVVLFVPFLIALALNGIEKGFWCRYLCPLGG
                     MLGLFSKFSPFKRKVERESCIECGICAQNCPVGAINPTDGFSSDPAECIQCTECAARC
                     PKYVIRFHGEKIFKSHEFDPDRRELLGIGGAAAAAAAIFAGMNSVNSQPATAETLLPP
                     GAVRDAANDGIKPFLSLCTRCGQCIAACPQKIIKPSLGKGDWTAALTPIVDLSANFCR
                     PDCNRCGQVCPSGAIPQFTVTQKQQAKIGVARVDPSQCIRCYRCVSACPYQAFEQMAD
                     LQTGQIFPHVETEKCNGCGLCLTVCPAREAGAIKIFPLQKG"
     gene            complement(2133..2981)
                     /locus_tag="PKV59_06280"
     CDS             complement(2133..2981)
                     /locus_tag="PKV59_06280"
                     /inference="COORDINATES: protein motif:HMM:NF022059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQP46396.1"
                     /translation="MWNKPTLCDNVKTWAYFPAIFLRGGKTFAEIGSKSTGGSFLLAL
                     EGDSLDPCLVEVPAGMKIRNILSFFGNKNILKEVKAVQAGGITGQILPVDRLDYPFDF
                     SLRTHANTRLGSMLKVYNSHHCVVRTAANALKVSALEACGRCLTCRVGISRLIDLMEK
                     ITSGEGKDVDPELLKEVAAAVRDSSMCPLGAEAPIALLTSLKYFPEEYEAHLNGSCPA
                     GRCPKLTDYLIREDQCTGCGDCQKVCPVHAIEGTEKNPRMIDPIQCIRCGRCPEVCPQ
                     GAIHYE"
BASE COUNT          812 a          835 c          674 g          718 t
ORIGIN      
        1 gtttttcttc gttataagcc ggtaagaccg ctaccagttt tgccatgcct tttcccttcc
       61 ttcatctgat tgatcttttt cggatcccgg actcttcaca atctttgtca taaaaaagat
      121 ttcgacccga atcgccgccc gtttatccct tttgcaacgg aaatattttt atcgcaccag
      181 cttcccgagc cggacagaca gtcaggcata atccgcaacc gttgcatttt tcggtttcga
      241 catgaggaaa aatttgaccg gtttgaagat ccgccatctg ttcgaaagcc tgatatggac
      301 aagcgctgac acaacggtaa caacgaatac attgagaagg gtccacgcgt gccaccccga
      361 ttttggcctg ctgtttttga gtgacagtga actgcgggat cgctccgctc ggacaaacct
      421 gaccgcaccg gttacaatcc ggccggcaga aattggcgct gagatccacg atcggggtca
      481 gtgcggcggt ccaatcgcct tttcctaaag aaggttttat gattttttgg ggacatgccg
      541 caatgcactg cccgcagcgc gtgcacagcg acaggaacgg ttttatgccg tcgtttgcgg
      601 catcgcgaac agcacccgga ggaaggagcg tttccgccgt cgccggttga gaatttaccg
      661 aattcatccc cgcaaaaatc gcggctgcgg cagcagccgc cccgccgatt cccaacaatt
      721 cgcggcggtc cggatcaaat tcatgacttt taaagatttt ttcaccatga aaccggataa
      781 catatttagg acagcgagcc gcgcattccg tgcactgaat acattccgcc ggatcggacg
      841 aaaaaccgtc cgtgggattg atcgctccga ccgggcagtt ttgggcacaa attccgcatt
      901 cgatacaact ttcccgctcg acttttctct taaaaggtga gaacttggaa aagagaccca
      961 acatcccacc taacggacag agataacggc accaaaagcc cttttcaatc ccgttcaatg
     1021 ccaacgcaat caagaagggg acaaaaagaa caacgatacc gatccaatca aacgaagcaa
     1081 aatctttttc cccggagtaa gcaaacaaag cagcaaaagg tcgggtcaaa agagcaatgg
     1141 gatccgtcca aaaaaaggcc aagcttccca tcgccgccag aagcaaaatc agtacaaaga
     1201 aaatctgttt gagattgcgt aatcgttggc ttttgatccg ccgtccgttc ggtccgaacg
     1261 actgcagcac ggtcccggtc ggacagatcc atccgcacca aaatcttccg aaaagaatcg
     1321 tcagaatgag gatcgccagc gccggcaggt agttgatcgc ctccggatga cccgagacca
     1381 gaacaacact cccttgcaga ggatccatca gagaaaacaa ttgatttgga aaaccctgaa
     1441 tttgaattcc gaagcctgac attaaaatca ataagataaa aagcgacaga aaaaacaacc
     1501 tgcttcgaga acgtgcggaa actcttccga ctttttttgc cttaacttgg tcgccgattc
     1561 ccaatccgag actttgcgtg accgcacccg tcgggcaata tttaatacag atatcgcagg
     1621 aatcacatgc cgatgcatgc gaaccgtttg ctgtcgtaac gacccgattt tctccgttcc
     1681 cccgaaattg caacacgccc gcactttttt cccgacatgc ttggacgcac gcaccgcatt
     1741 gaatacaacc cttctgaagg ctttgagtgc gataagaagg ttgggaaata ccgtaagtcg
     1801 cagccagccg ctgaagcgac ggcgtatcgg gggagatcga aagtaacatt tcggccgcct
     1861 gacgacggga attcaatact tcttccgtat ccgtcctcac ccgcattccg tcaatcgcca
     1921 aagtatcgca ggctgtcacc aaacgttgtg atccgttcgg aaacgttacc gataccagac
     1981 agacccgaca agccccggtc ggtgtcaaat cttttacgac acaatatgtt ggaatatcga
     2041 ttcgatttgc ttgggctatt tgcagaatcg tcatcccatc cttcgcttcc acgcgttttt
     2101 gatcaaaggt aagatagatc ttgctccgtt cgttattcat aatgaatcgc tccctgagga
     2161 caaacttccg gacaccgacc gcagcggata cattgaattg gatcaatcat tcgggggttc
     2221 ttttccgtgc cttcgattgc atgaaccgga cagacctttt gacaatctcc gcatcccgta
     2281 cattgatctt cccgaatcag ataatcggtc agtttcggac aacgacccgc cggacaactt
     2341 ccgttcaaat gggcttcata ttcttccgga aaatatttta aagaagtcag caacgcgatc
     2401 ggcgcttccg cacccagcgg gcacattgaa ctgtctcgga ccgccgccgc gacttccttc
     2461 aacaattccg gatcgacatc ttttccttct ccggaagtta ttttctccat taaatcaatc
     2521 aagcgggaaa tgccgacccg acaggtcaga cagcgaccgc aggcttccaa agcacttact
     2581 tttaatgcat tggcggctgt acggacgaca cagtgatgag agttatacac tttcagcatc
     2641 gatcccagcc gggtatttgc atgtgttcgc agtgaaaaat caaacggata atccaaacgg
     2701 tcaaccggaa gaatctgacc ggtaattccg ccggcctgaa ccgctttcac ttccttgaga
     2761 atgttcttgt tgccgaaaaa cgacagaata ttccgaattt tcatgcctgc cggtacctcg
     2821 accaaacaag gatcgagaga atctccctct aaagccaaca aaaacgatcc tccggttgat
     2881 ttggatccga tttccgcaaa agtctttcct ccccgcaaaa agatcgccgg gaaataagcc
     2941 caggttttca cgttatcaca taaggtcggt ttattccaca accccttttc ttccggttct
     3001 ggaataaacc gaccttatgt gataacgtga aaacctggg
//
LOCUS       DAOMIV010000241         3034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_34679_length_3034_cov_2.967439, whole genome shotgun sequence.
ACCESSION   DAOMIV010000241 DAOMIV010000000
VERSION     DAOMIV010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34679_length_3034_cov_2.967439"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..767)
                     /locus_tag="PKV59_06285"
     CDS             complement(42..767)
                     /locus_tag="PKV59_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46397.1"
                     /translation="MIAIQTVGLTKKYGKITAVDNLNISVNKGELFALLGLNGAGKTT
                     TIKMLSCLTRPTGGDAILLGDSIISKPLSIKQKTNISPQETAIAPNLTIRENLELIAG
                     IYGQDKKTAHQNAICMANRFELDNLMTKKVKALSGGLQRRLSIAMALISEPQILFLDE
                     PTLGLDVLARRELWSAIKSLKGKVTIILTTHYMEEVEELSDRIGILSSGKLLATGTIS
                     ELMAQTNTNKFEDAFVILAKGGR"
     gene            complement(767..988)
                     /locus_tag="PKV59_06290"
     CDS             complement(767..988)
                     /locus_tag="PKV59_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HQP46398.1"
                     /translation="MSAPKGKYAWTVKVGEKGQIVIPKEAREIFNISPGDTLILLGDK
                     KRGIAIPPKDVILKLAEVFGDSLPDQDTE"
     gene            1338..1946
                     /locus_tag="PKV59_06295"
     CDS             1338..1946
                     /locus_tag="PKV59_06295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQP46399.1"
                     /translation="MLLEDDAIVLFQGDSVTDAGRDYTDETDLGTGYPMMAASYFSAT
                     YPEKDVKFINKGISGNRVRDLKDRWKTDCIDCNPTWVSILIGINDCWRRYDSDDPTPK
                     ENFEADYRYILRQTRDKLHAKLILCEPFVLPVSEDREAWREDLDPKISVVRKLAAEFN
                     AIFLPLDGIFAQAAAKREANFWLPDGVHPSLAGHALIAKNWL"
     gene            complement(2109..>3034)
                     /locus_tag="PKV59_06300"
     CDS             complement(2109..>3034)
                     /locus_tag="PKV59_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HQP46400.1"
                     /translation="SDYEVAFGQGILQKYSPIDLDFEEKSPKAHIIDGDYNVGIKMEN
                     SFALISKASGKLVSIQSEGTELLRSPVSPDFWRAPTDNDLGNGNTFRWAQWKIASLYQ
                     KCEHVSVDPERAQVKSFFFMPANPKIHCSMEYSFFKKNFVRIRMHLDPVKGDAPCVGI
                     MFKMPGKWNRLSWYGNSQSEAYSDRQSACRIELCEGSVDRQYVPYLHPQECGNKTQLR
                     FFTVTEDRGKGLRLSSDSPFEASALPYTAHEIENASNILHLPPRNETVVGAYKQKCGV
                     GGDDSWGAPVHEEYLIKTDEGIDFTIYLQIL"
BASE COUNT          850 a          602 c          687 g          895 t
ORIGIN      
        1 caaaatttct ttcccatttc gttttgagaa aatataagct ctcatcgtcc accttttgcg
       61 aggataacaa atgcatcttc aaatttgttt gtgtttgtct gtgccatcaa ttcggagatc
      121 gtaccggtgg cgagtaattt tccgcttgac aaaatcccga ttcgatcgga aagttcttca
      181 acctcttcca tataatgtgt cgtaagaatt atcgttactt tcccttttag tgatttaatc
      241 gctgaccata attcacgtcg agccaataca tcaagtccaa gagttggttc atctaggaag
      301 agaatttgcg gttcagatat caaagccata gcgatactga gtcgtctttg taagccaccg
      361 gataaagctt ttaccttttt cgtcattaaa ttgtccaatt cgaatctatt tgccatgcag
      421 atagcgttct ggtgagcggt tttcttgtct tgtccataaa taccggcgat gagttcaaga
      481 ttttcacgga tagtaagatt cggagcaatc gctgtttcct gtggtgagat atttgtcttt
      541 tgtttaatcg ataatggctt agagataatg ctgtcaccga gcagaatagc atctccgccg
      601 gttggacgtg tcagacaaga aagcatttta atggttgtgg tctttcctgc tccgttcaga
      661 ccgagcaagg caaacaactc acctttattg acagaaatat tgagattgtc tacagcagtt
      721 attttgccat acttttttgt cagaccaaca gtttgtatag cgatcattat tctgtatcct
      781 gatcaggcaa ggaatctccg aagacttccg caagtttcag tatgacatct ttgggaggaa
      841 tcgctattcc tcgtttttta tcgccaagca aaatcaatgt atcgccgggc gagatattga
      901 aaatttcacg tgcttctttg ggaattacaa tttgtccctt ttctcccact tttactgtcc
      961 atgcgtattt tccttttgga gcgctcatga ttgcctgcct cttttatgat tagtaagaat
     1021 agtataataa gtatgaatag tataactatt catacaattt tgtcaagtat aaaaatgaag
     1081 aattgatcga gataaaatcg ggaattgatt ttatataaag aatatgattt gtttaaaaga
     1141 atgaactacg aataggatta atcaaaacgg caaaagatag aaagagtacc tttggaggga
     1201 acaaggaaaa aagatttcaa tttaggttca tacagctgtg gatagtgcag attatagtca
     1261 atgaaaccta tttttccgga tacaatcgat agggaagtat agccagtcgg agaaatctca
     1321 agaaaggaat aaagtgcatg ttgttggaag atgatgcgat agtgttgttt cagggagaca
     1381 gcgtaaccga tgcgggacgc gattatacgg atgaaaccga tttgggaacg ggctatccca
     1441 tgatggcggc atcttatttt tctgctacct atccggagaa ggatgtgaag tttattaata
     1501 aaggaatcag cggaaatagg gtaagagatc tcaaggatcg gtggaagaca gattgtattg
     1561 attgcaaccc cacttgggta tcgatcctga tagggataaa cgattgttgg agacgatatg
     1621 acagcgacga cccgacgccg aaagaaaatt ttgaggccga ttatcgctat attttgcggc
     1681 aaacaagaga taaacttcat gcgaaactca ttttatgtga gccgtttgtc ttacctgtgt
     1741 ctgaagatag ggaagcatgg cgagaagact tggatcccaa gatatctgtg gtaagaaaac
     1801 tggcggcgga atttaacgcc atattcttac cgcttgacgg aatattcgcc caggcagcgg
     1861 ctaagaggga agcgaatttt tggttgccgg acggcgtaca tccttcgctt gccggacatg
     1921 cgcttatcgc aaagaactgg ttataagccg tggaagcttt ataaatagag gacagctaaa
     1981 agtgacggtt tttatcgccg gctgaatgat tatttcctgt aatcaggttt gatcgagaaa
     2041 gaaaagtgat tatttccgga ttttttatca gcctcttgat ttcctcaaaa ggctgataaa
     2101 aagattcctc aaagaatttg aaggtagatc gtaaaatcga ttccctcatc tgtttttatt
     2161 aaatattctt catgaaccgg tgctccccaa ctgtcgtccc cgccgacacc gcatttctgt
     2221 ttataagcac cgactacggt ttcgttccga ggcggtaagt gcaaaatatt gcttgcattt
     2281 tcgatttcat gagcggtgta gggcaaggcg gatgcttcaa agggactgtc actcgaaaga
     2341 cgcaggcctt tgccgcgatc ttccgtcacg gtgaaaaacc gcaattgagt tttgttaccg
     2401 cattcctgtg ggtggagata agggacatat tgccggtcga ccgatccttc acaaagttca
     2461 atccggcaag ccgactgacg atcggaatag gcttcggatt ggctattccc gtaccaagat
     2521 aaccgattcc attttccggg catcttgaac ataatcccga cacaaggagc gtcgcctttc
     2581 accggatcca gatgcattcg gatccgaacg aaattctttt taaaaaagga gtattccatg
     2641 ctgcagtgaa ttttgggatt ggccggcatg aagaaaaagg atttgacttg tgctcgctcg
     2701 ggatcaacgg agacatgctc acatttctga taaagagaag cgattttcca ttgtgcccag
     2761 cgaaaggtgt ttccgttccc gagatcattg tcggtcggtg cacgccaaaa atccggagag
     2821 accggacttc ttaatagctc cgtcccttcg ctttgaatgg atacgagctt cccgctggct
     2881 tttgaaataa gcgcaaaact gttttccatt ttaatgccga cattgtaatc accgtcgatg
     2941 atatgagctt tcggcgattt ctcttcgaaa tcaagatcaa tcggagaata tttttggaga
     3001 atgccttgtc cgaaagccac ttcataatcg ctgg
//
LOCUS       DAOMIV010000242         3023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_34870_length_3023_cov_4.843666, whole genome shotgun sequence.
ACCESSION   DAOMIV010000242 DAOMIV010000000
VERSION     DAOMIV010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34870_length_3023_cov_4.843666"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1465
                     /locus_tag="PKV59_06305"
     CDS             158..1465
                     /locus_tag="PKV59_06305"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HQP46401.1"
                     /translation="MNAVDIIIKKRDSKALTDEEIRFFIDGYTEGKIPDYQAAAWAMA
                     VLCRGMTARETTALTRAMAESGEMISLKGIADIAVDKHSTGGVGDKTTLIVQPIVTAC
                     GLPVAKMSGRGLGFSGGTIDKFESIPGMRLNLSKEEFLDQLKQYGTVLSGQSADLAPA
                     DGKLYALRDVTGTVPSIPLIASSIMSKKIAAGTPLILLDVKVGNGAFMETLEKAEKLA
                     RLMVSIGKKFGRTVKAELTDMNQPLGYAVGNALEVQEAVRMLRGEKSAPDLYEHCIES
                     SALMIKMGRKAKSVDEARHMAEDAIHTGKALESLKLLVRLQGGDVSYIDDLAKFPKAP
                     IIRDLYARSDGWLSEINARMVGEASVLLGAGRVTKADRIDHRVGIIVHHKVGDPIKKG
                     MPLFTVHANNEEKLNQAAELLNNACKYSESEVEKLPQFYGLIE"
     gene            complement(1473..>3023)
                     /locus_tag="PKV59_06310"
     CDS             complement(1473..>3023)
                     /locus_tag="PKV59_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46402.1"
                     /translation="IQFNNSLILGLQGLNRAADILKQMESRIWVENRSQQILELKLRL
                     DEINNAFRALSGMIEQANSFVRQGDFRSAERILNSFQTGEQDKWPDWLKIQKENAENE
                     IRDLSEKYHLIQSRFTKDSSGNGEVIEKVNRILDPKTSDPALISELSNELNQYKTILE
                     KEIKVDPEKNPYIKQINYLLGLLNWVESSDNTIRGYGIRGSEISLDAIYAIRKNARDF
                     FEQIPAELIGIQPGFAARNKWLEQRTQAHQLLTQLHESPKKSRQKRSERGQTIKNTIR
                     ELERMDLLPREAESLQRYKDTRKKKASRKHRLVGGLISVILILGALYMASPKLIPLLT
                     PSPTLSLTPTLSETPTLTLTNTVTPSPTLTATPTLTMTPTATFTFTPTPVGLTGLINN
                     NRVGVYELPSGMSVMISDGYLEPGTKVNVIRYCEPDFNKGEIWALIFYPSKNRNTGWI
                     RVRLPDSPDFVSLSDINMPAIEVIRANPYLKIECPTLSYQRMPGDEITLTPAVTQTPE
                     EKISTEKP"
BASE COUNT          764 a          642 c          777 g          840 t
ORIGIN      
        1 ccgggtgcgg ttgtcgggaa cgacactcct tatgaatgga cctgccaagg cggaaagccc
       61 gttattaccg gtcaaggggt gaccgtggat gaacagggat ataacgctct gatttggtat
      121 ccggtaccga gacaataaac cttagcggag tttttttatg aatgcggttg acattattat
      181 taaaaaaaga gattccaaag cattaaccga tgaggagatt cgatttttta ttgacggtta
      241 taccgaaggg aaaattcccg attatcaagc ggcagcttgg gcgatggcgg ttttgtgccg
      301 cgggatgacc gctcgggaga cgacggccct aaccagagcg atggcggaat ccggcgagat
      361 gatatctctc aagggcattg cggatatcgc ggttgataaa cattcgaccg gaggagtggg
      421 cgataaaacg acgctgatcg ttcaacccat tgttacggct tgcggtttgc cggtggcaaa
      481 gatgtccggg cggggattgg gattcagtgg aggcaccatt gacaaattcg agtctattcc
      541 cggaatgcga ctgaatttat cgaaagagga attcttagac caactgaaac aatacggtac
      601 ggttctttcc ggacaatcgg cggatctcgc tccggctgac ggaaagctat atgcgctccg
      661 agacgtcacc ggaaccgttc cgtctatccc cttgattgct tcatccatta tgagcaagaa
      721 aatcgccgcc ggaaccccgc ttattttatt ggatgtcaaa gtcgggaacg gcgcttttat
      781 ggaaacactt gaaaaagcgg agaaactggc aagattaatg gtgtccatcg ggaagaagtt
      841 cgggagaacc gtgaaagccg aattgaccga tatgaaccaa ccgttgggtt acgccgtcgg
      901 gaatgcgttg gaagtacagg aagccgtgcg tatgttacgc ggagaaaaat ccgctccgga
      961 tttatatgag cattgcatcg aatcttccgc gttgatgatt aaaatgggta ggaaagcgaa
     1021 gtccgttgat gaagcgcgtc atatggcaga agatgcgatc cataccggaa aagctttgga
     1081 gagtctaaag ctgttggttc gtctccaggg cggggatgtt tcttatatcg atgatctcgc
     1141 taaattcccg aaggcgccca ttattcgaga tttatatgcc cgatcggatg gttggctttc
     1201 ggaaatcaat gcccgcatgg ttggtgaagc atccgttttg ttgggcgccg gtcgggttac
     1261 caaagcggat cgaatcgatc atcgagtcgg gattattgtg caccataaag tcggcgatcc
     1321 gatcaaaaaa ggcatgccgc tttttacggt tcatgccaac aatgaggaaa agctgaatca
     1381 agccgctgaa ttattaaaca atgcctgtaa gtacagtgaa agcgaagtcg aaaaactgcc
     1441 gcaattttac ggactgatcg aatagcggaa gcctacggtt tttcggttga aattttttcc
     1501 tcgggggttt gcgtaacggc gggtgttaaa gtgatttcat cacccggcat acgttgataa
     1561 gaaagggtcg ggcattcgat cttaaggtat gggtttgccc ggatcacttc gattgccggc
     1621 atatttatat ctgaaaggga aacaaaatcc ggtgaatccg gcaaacggac tcgaatccat
     1681 ccggtgttgc gatttttact cggataaaag attaatgccc agatttcgcc tttgttgaaa
     1741 tcgggttcac aataccgaat cacattcacc ttggttcccg gttccaaata tccgtcgctg
     1801 atcataactg acatgccgga aggaagttca taaaccccga cacggttatt attgatcaaa
     1861 cctgttaacc caaccggtgt aggcgtaaac gtgaaagtgg ctgtgggcgt catcgtcaga
     1921 gtcggtgtcg cggttaatgt aggtgacggt gtaacggtgt ttgtcagagt caacgtcgga
     1981 gtttcggata aagtcggcgt cagggaaaga gtcggcgaag gtgtcaacag tggaatcaat
     2041 tttggcgaag ccatataaag tgcgcccaga attaagataa cggaaatcaa tcctccgacc
     2101 agccgatgtt ttctacttgc ttttttcttt cgcgtgtctt tataacgttg taaggactcg
     2161 gcttcccgcg gaagaagatc cattcgttcc aattcgcgga tggtattttt aattgtctgt
     2221 ccccgctcac tccgtttttg tcttgatttc ttcgggcttt catgtaattg ggtcaataat
     2281 tgatgagctt gcgttcgttg ctcaagccat ttgttccgtg cggcaaaacc cggctgaatt
     2341 ccgatcaatt ccgcgggaat ttgctcgaag aaatctcttg cgttttttcg aattgcgtaa
     2401 atggcatcca gactgatttc cgatccgcga atgccatatc cgcggatcgt gttatcggac
     2461 gattcaaccc aattcaacaa acctaacaaa taattaattt gcttgatata aggatttttt
     2521 tcgggatcga ctttgatttc tttttccaaa attgttttat attgatttaa ttcattcgaa
     2581 agttcggaaa tcaatgcggg atcggatgtt ttagggtcca atatgcgatt gactttttca
     2641 atgacctccc catttccgga cgaatctttc gtgaaacggc tttggatcag gtgatatttt
     2701 tcgctgagat cacggatttc attttcggca ttttcttttt gaatcttaag ccaatccggc
     2761 catttatctt gctctccggt ttggaaggag tttaggattc gttccgcgga tcgaaaatct
     2821 ccctgtctta cgaaggaatt cgcctgttcg atcatgcctg aaagtgcgcg aaaagcgttg
     2881 ttgatctcat cgagtctcag tttcaattca aggatttgct ggctgcgatt ttcgacccag
     2941 atccgcgatt ccatttgttt caggatgtcg gccgcccggt ttaagccttg cagacctaaa
     3001 atcaggctgt tattgaattg aat
//
LOCUS       DAOMIV010000243         3014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_35012_length_3014_cov_4.856032, whole genome shotgun sequence.
ACCESSION   DAOMIV010000243 DAOMIV010000000
VERSION     DAOMIV010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35012_length_3014_cov_4.856032"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..549
                     /locus_tag="PKV59_06315"
     CDS             160..549
                     /locus_tag="PKV59_06315"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQP46403.1"
                     /translation="MKIDRTFLRNLILYGIIGAISAGFDALVFYLLFDKLAINKFVAN
                     VISVHFGIFLSFFLNSFYNFKKTDKMGKRFFSFYLTGLFGLGLSTLILYAGGRLRIDV
                     MATKVFSVVFVALVQFIINRAVAFGDK"
     gene            582..2825
                     /locus_tag="PKV59_06320"
     CDS             582..2825
                     /locus_tag="PKV59_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQP46404.1"
                     /translation="MNRTEILLGILAGLFISQKIRVPLPIWLIFLLLFAVLAVFCGKK
                     SSKYVLDQPVKKAAMIALTAAFCCLGGLRGSVTNQPLPSGHLSEVSGEEVSFKGIINR
                     PVTRSDSQTRLRIRLVRNEDSDSDPDRGECIVVLNSLTNSFEYGDLLSVRGKLTKLDA
                     SAPNSYGSYLERSGISGILYNPEIELIAKQQGNPILNRIFRLQSYLLQKTYDIFPEPE
                     NSLMAGILLGDKSRIPAEIEEAFRNTGTAHIIAISGMNFSVLIWMLLSVLRAFHKRWW
                     TPLILLPFIFFYTILTGANPAIVRAGIMGGIALIGMSIGREKGGVDSLLLSVAAMAMF
                     QPKILFDVGFQLSATATLGILIFNPPLNSLIEKGFTAIHLKNNVQKILLDTLNEILIT
                     SFSAQIFTTWIIAASFHQFAIFSLIVNMLIAPFQNLIMVGGVFALFLSILSESLGKIA
                     GLIVDPLPALTIRIICFFGKFDWASQYSDLSNLAAWIIIFSILTVWINRNRWIRQKKE
                     TLIQAGLTVLALFTLVCWRQVLAKADHRLHLTFTGGTNTSSILIRLPESQKILVANGV
                     TNFQAQNLLKKNPFDPADYAAAILDFQEEWMADEYLRSDDEKPEWLILNGRNCSLSAE
                     QTNPTPVVLNEGFQLRTDPFILTLQQEYLKRKRWIISYRSLTIDIPNGIPLHRIDTLN
                     DPNPSVSIILLGKKDNEKEWEEYLSQTYCHGSSCSGKPILLSTNEYGTIEVISDGEKI
                     WLVQQSS"
BASE COUNT          838 a          623 c          628 g          925 t
ORIGIN      
        1 cggatttgtt atcgcgcggg aagcgggcgc tgtggtcagt aatctcaaag gcgatccgaa
       61 ttgctttcat gtaccctttg aatttttgat cgccaatccg atccttcatc ctttgttgtt
      121 aaaagaaatc gaactgatga tagaatcgaa acagacagaa tgaaaattga tcgaacgttt
      181 ttgcgaaatc tgattctcta tggaattatc ggtgcgatca gcgccggttt tgatgcgctt
      241 gttttctatc tgctttttga caaattggcg atcaacaaat ttgtcgccaa tgtaatcagc
      301 gttcatttcg gcattttttt aagttttttc ctcaatagtt tttataactt caagaaaaca
      361 gacaaaatgg gcaagcgatt cttctctttt tatttaacag gattattcgg gctcggctta
      421 tccacattga ttctttatgc cggtggaaga ttaagaatcg atgttatggc caccaaagtc
      481 ttttccgttg tttttgtagc gctggtccaa tttataatca accgagctgt tgcgttcggc
      541 gacaaataaa aaatttgatg aaattcattt gagaaatttt catgaacaga accgaaatat
      601 tgctcggtat actggcggga ttatttattt cacagaagat ccgtgtcccc ttaccgatat
      661 ggctgatatt tttgcttttg ttcgctgttt tggctgtatt ctgcggaaag aaatcttcaa
      721 aatacgtcct tgatcaaccg gtaaaaaaag cggcgatgat tgctttgacg gctgctttct
      781 gctgcctcgg cggattgcgc ggatcagtga caaatcaacc cctcccttcc ggacatttat
      841 ccgaagtctc cggtgaagaa gtttccttta aagggatcat caaccgtccc gtgacacgat
      901 cggattcaca gacacggttg cggattcgtt tggttcggaa tgaagattcg gattctgacc
      961 ccgatcgggg agaatgtatt gtggttctca attctttgac aaattctttt gaatatgggg
     1021 atcttctttc cgtccgcgga aaattgacaa aactcgacgc ctctgcccca aattcttacg
     1081 gaagttatct ggagcgatcg ggtatatccg gaattttata taatcccgaa atcgaattga
     1141 ttgcaaaaca acaggggaat ccgattctca atagaatctt tcggcttcaa tcctatttgc
     1201 tgcaaaaaac ttatgatata ttcccggaac ctgaaaattc gttgatggcg gggatattgt
     1261 tgggagataa gagccgtatt ccggcggaaa tcgaagaagc atttcgaaac acgggaaccg
     1321 ctcatattat cgcaatcagc gggatgaatt tttccgtttt gatctggatg cttttatccg
     1381 tcttacgagc gtttcataaa cgctggtgga caccattaat cctattgccg tttattttct
     1441 tctatacgat tcttaccggg gcgaacccgg cgatcgtccg agccggaatt atgggaggaa
     1501 ttgccttgat cggaatgagc atcggcagag agaaaggagg agttgattca ttgcttttat
     1561 ccgtcgctgc gatggcgatg tttcaaccga aaattctttt tgacgtgggc tttcagttat
     1621 cagctacggc aactctggga attttgattt ttaatccgcc attgaatagt ctgatcgaaa
     1681 aaggatttac agcaattcat ctgaaaaata atgtccaaaa aattcttctg gatacgctga
     1741 acgaaatatt gatcacttca ttttccgctc agatatttac tacttggatt attgccgcat
     1801 catttcatca attcgcaata ttttccctga ttgtcaatat gcttatcgcg ccgtttcaaa
     1861 acctgatcat ggtcggaggt gtgtttgcgt tattcctttc aatactatcc gaatcgctgg
     1921 gaaaaattgc cggcttaatc gttgatcctt taccggcatt aaccatccga ataatctgct
     1981 ttttcggaaa atttgactgg gcatctcaat acagtgatct ttcaaatttg gctgcctgga
     2041 tcattatttt ttcgatcctg acagtttgga tcaatcgaaa tcgctggatt cgacaaaaga
     2101 aggaaactct gatccaagcc ggcttaaccg ttcttgcgct ttttaccctt gtttgctggc
     2161 ggcaggtttt ggcaaaagcg gatcaccgac ttcatctgac ttttaccggc ggcaccaata
     2221 caagctctat cttgattcgc ttgccggaat ctcaaaaaat tctggtggca aacggagtta
     2281 caaattttca agcccaaaat cttctgaaaa agaacccctt tgacccggca gattatgccg
     2341 ctgcaatttt ggatttccaa gaagaatgga tggcagacga atatctgagg agtgatgatg
     2401 aaaagcctga atggttgatt ctcaacggta ggaattgcag tttatccgcc gaacaaacaa
     2461 acccaacgcc tgttgttttg aacgaaggat ttcaactccg aacggatccg tttattctca
     2521 ctttgcaaca agaatatctc aagcgaaaaa ggtggataat aagctaccga tctttgacaa
     2581 tcgatatccc aaacggtatc ccgcttcatc gaatcgacac cttgaacgat ccgaaccctt
     2641 ccgtttcaat cattctgtta gggaagaaag acaatgaaaa ggaatgggaa gaatatttat
     2701 cacaaactta ttgtcatggt tcctcctgtt cggggaaacc tattttattg agtacgaatg
     2761 aatacggaac catcgaagtt atttccgacg gcgaaaaaat ctggctggtt cagcaatctt
     2821 cttaaccata agtttcgtat cgatcccaca gagtaatgag aaacgtcgat ttattaattc
     2881 tttccataaa atttacaaaa agccgaaaac tcttacaaaa atttactctg taaacagacc
     2941 ctttaatcgt attttttgat agaatcaatc tatacaaaag aaagagtgaa gggaggataa
     3001 tatgttgccc cttg
//
LOCUS       DAOMIV010000244         3014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_35024_length_3014_cov_3.190605, whole genome shotgun sequence.
ACCESSION   DAOMIV010000244 DAOMIV010000000
VERSION     DAOMIV010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35024_length_3014_cov_3.190605"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..960
                     /locus_tag="PKV59_06325"
     CDS             262..960
                     /locus_tag="PKV59_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46405.1"
                     /translation="MLELLLPLNIVLIILILLVSLFLLSKSADFLVDNAVKLSKIIKI
                     PEMVIGATIVSFGTTLPEFSASTISALRGNFGFALGNAIGSIITNTSLVLGVGALFGA
                     IPVTRKASQKLSILNIVILLLILPTIPRVLRNGEGTIPQWMGVVLLVLVPFYVLFLIA
                     QEKRSPEIEISDPEEAESHSNLFLTALLVLGAAAFVAFCASVLVASAEIIATRIGISD
                     ILISATISFRDQCA"
     gene            953..1264
                     /locus_tag="PKV59_06330"
     CDS             953..1264
                     /locus_tag="PKV59_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46406.1"
                     /translation="MPELTTAVSAAKTGHGGLAIGNVMGANILNILWVTGASIAVIPG
                     GITVPNSFYLVHFPAMIAVLAIFGYFAYNKNRNEINHKEGILLTGIFGIYLLANVIFT
                     L"
     gene            1289..2632
                     /locus_tag="PKV59_06335"
     CDS             1289..2632
                     /locus_tag="PKV59_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HQP46407.1"
                     /translation="MKTRRKILLTNTQIIALGFFLVILAGSILLMLPISSKNGSWTPF
                     IDALFTATSATCVTGLVVFDTFTHWTVFGQIVILTLIQVGGIGLMTIVSGAYILLNKR
                     IGIQTRQLLMTSAGNLQLSGVVRLIKRILFGTLLFEGTGTVFLATQFCPEMGIGKGIY
                     YAIFHSVSAFCNAGFDIMGFKGEYSSLISYQSNWIVNLTIILLIVIGGIGFYVWSDTV
                     KNRFRFRRLSLHTQITLTTTAFLLAFGTLLYFFFEKNFSMQGMTTDNRFLASLFMATT
                     PRTAGFNTIDLTKLSEPGNLLTMILMLIGAGSGSTGGGIKVTTFAVLMLNLFFMARGY
                     ENVTIFKKRLESNSFKQAGSILCCYLLAVIAATMWICSQEIYSATDVAFECFSAIGTV
                     GLSKGLPALMKPISKIILILLMYGGRLGGISFLMIFTRQEKERPLKRVSKKILLG"
     gene            2648..>3014
                     /locus_tag="PKV59_06340"
     CDS             2648..>3014
                     /locus_tag="PKV59_06340"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HQP46408.1"
                     /translation="MKSFLVIGMGSFGRHLAEKMIELGNSVIAVDKNQSLIEEIADRF
                     PDSYYGDCTNPAVLGNLGVNNFDVCFVAIGEDFFSSLEITSILKELGARYVVSKAKGE
                     RQSKFLRTIGADEVIYPEKE"
BASE COUNT          793 a          569 c          699 g          953 t
ORIGIN      
        1 aattgaaaaa aattatgata agatagatcg agacggatga aagcggtcga acagattccg
       61 tctctgaaat cagttgtcaa gaggatgaaa taacttcttg accaaaatat aaaaatatta
      121 tggattttac ggttaccgta cagcgattac ggtaagattc ggactttttg ggtgggaagt
      181 ctttttataa aggcttccca tttttttatt ccggaaacgg agtggaagcg gaaaaaattc
      241 actgacaagg gaggtttaca aatgcttgaa ttattattac cgctcaatat tgtcttaatt
      301 attcttattt tattggtatc cttatttctt ttatccaaat ccgcggattt tctggtagat
      361 aatgcggtga aactttcgaa aattataaaa attccggaaa tggttattgg tgccacgatc
      421 gtttcttttg ggaccacctt acctgaattt tccgcttcca ctatttccgc actccgaggg
      481 aatttcggat ttgcgcttgg aaacgcgatc ggttccatta ttactaacac cagtttggtt
      541 ttgggtgtcg gcgccctgtt cggagcaatc ccggtaactc gtaaagcttc gcaaaaatta
      601 agtattttga atattgtaat cttacttctg attttgccga cgattccccg agtgctccgt
      661 aatggcgaag ggacgattcc tcaatggatg ggggtggtct tgttggtttt ggtccccttt
      721 tatgtgctgt ttttaattgc acaggaaaaa aggtctccgg aaattgagat ttcggatcct
      781 gaagaagcgg aatctcattc caatttattc ttgaccgccc tgcttgtttt gggagcggct
      841 gcgttcgtcg cattttgtgc ttcggttctg gtagcttccg ccgaaatcat cgcgacgaga
      901 ataggtatat cggatatcct tatctcggcg acgattagct ttcgggacca gtgtgcctga
      961 actgacaacc gccgtatcag ccgctaaaac aggtcatggc ggactggcga tcgggaatgt
     1021 gatgggagcc aatattctga atattctgtg ggtaaccgga gcatcgattg cggtgattcc
     1081 gggcggaata acggttccaa atagttttta tctggttcac tttccggcaa tgattgcggt
     1141 gttggcgata ttcggttatt ttgcctacaa taaaaaccga aacgaaatca accataaaga
     1201 gggaatttta ttaaccggaa tctttggaat ttacttattg gctaatgtca tcttcacact
     1261 gtaataaatc caaatataat tagtgtgaat gaaaacgcgc agaaaaattc ttctgacgaa
     1321 cacccaaatt attgctttag gttttttcct ggtaatctta gcgggcagta tattattaat
     1381 gctcccgatt tcttccaaaa acggatcatg gacgcctttt attgatgcgc tcttcaccgc
     1441 gacaagcgcc acctgtgtga cgggtttggt cgtttttgat actttcacac attggacggt
     1501 tttcggccaa attgtgatcc tgacgctgat tcaggtcgga gggatcggct tgatgacgat
     1561 tgtcagcggc gcttatattc tgttaaataa aaggatcggc attcagactc gccagctgct
     1621 gatgacatcg gccgggaatt tacaattgag cggtgtcgtt cgcttgataa aacgaatact
     1681 ttttgggact ttattgtttg agggaacggg gactgtcttt ttggcaaccc agttttgccc
     1741 tgaaatgggg atcggaaagg gcatttatta tgcgattttt cattcagtat ccgccttttg
     1801 taacgccgga tttgatatca tgggatttaa gggagaatat tcttccttaa tttcttatca
     1861 aagtaattgg atcgtcaatc tgaccatcat tctgctgatt gtcatcggcg ggatcggatt
     1921 ttatgtttgg agtgatacgg ttaaaaatcg attccgattt cggcgtcttt ctctgcatac
     1981 tcaaattaca ttgactacga ctgcttttct tttggcgttt ggtaccctct tatacttttt
     2041 cttcgagaag aatttcagta tgcaggggat gacaacagat aatcgctttc ttgcctcatt
     2101 atttatggcg acaaccccga gaaccgcagg ctttaataca attgatctga caaaattgtc
     2161 agaaccgggg aatttattaa ccatgatctt gatgttaatc ggcgccggtt cgggttcaac
     2221 cggcggtgga attaaagtca caacctttgc cgttttgatg ctcaatctct tttttatggc
     2281 aagaggctat gagaatgtaa caatattcaa aaaaaggttg gaatcgaatt cttttaaaca
     2341 agccggttcc atattgtgct gctatcttct ggcagttatt gcggctacga tgtggatttg
     2401 ttcgcaagaa atttacagcg cgacagatgt tgcttttgaa tgtttttctg cgatcggcac
     2461 agtcggatta tcaaaggggc tgcctgctct tatgaaaccg atttccaaaa tcattttaat
     2521 tttattgatg tatggcggaa gattgggtgg aatctcgttc ctgatgattt ttaccagaca
     2581 ggaaaaagag cgaccgctca aacgcgtctc gaaaaaaata ttattaggat aagggacggg
     2641 ggaaaaaatg aaatcttttt tagtcatcgg tatgggatca ttcggtcgcc atctcgccga
     2701 gaaaatgatc gaactcggca atagtgtcat tgcggtagat aaaaatcaaa gcctgattga
     2761 agagatcgcc gatcgatttc ccgattctta ttatggcgat tgtaccaatc cggcggttct
     2821 cggcaacttg ggtgtaaata atttcgatgt ctgttttgtg gcaatcgggg aagatttttt
     2881 ttcttcgctt gaaataactt cgattttaaa ggaactggga gctcggtatg tcgtttcaaa
     2941 ggcaaaagga gaacggcagt cgaagttttt acgaacaatc ggagcggacg aagttatcta
     3001 tccggaaaaa gaaa
//
LOCUS       DAOMIV010000245         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_35405_length_2990_cov_4.178194, whole genome shotgun sequence.
ACCESSION   DAOMIV010000245 DAOMIV010000000
VERSION     DAOMIV010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35405_length_2990_cov_4.178194"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..976)
                     /gene="atpG"
                     /locus_tag="PKV59_06345"
     CDS             complement(89..976)
                     /gene="atpG"
                     /locus_tag="PKV59_06345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HQP46409.1"
                     /translation="MASNREMQNRIRTVESIGQLTRALQAVSASQVRVVRVLSENTLP
                     YTTLSWQVLTDLYHEQEFYNQIRAFQLPDVSKDVLIVFISADRGLAGSYPVNIFREVN
                     HLERKIQTKTKYITIGKKGRDMLQRRRKNIIADFSELSTATSFHDLKTVSEIVIDEFN
                     QERVGQVYLVYTEYESISRFTPTTKRLLPFVDPFLGQLDEKEEKHIRKGGCIFEGDPQ
                     VILKSLITRFIRMELFHAVTSSKASEHTMRMLAMNQATENSEELLQSLQLKYNKIRQE
                     NITKDILDIMGGSEVHSDG"
     gene            complement(980..2644)
                     /gene="atpA"
                     /locus_tag="PKV59_06350"
     CDS             complement(980..2644)
                     /gene="atpA"
                     /locus_tag="PKV59_06350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HQP46410.1"
                     /translation="MTEETIRNITADLFEKIDDFQPNYEVEDIGIVMEVGEGIARVSG
                     LPTIGSQELVEFENGARGIAFSLEKDTIGVIVLGNYFTIKEGSIVKKTGRIASIPVGE
                     GLLGRVINPLGEPIDGKGPIRFDGYRAIERIAPGIHERSDVNTPLQTGIKAIDSMIPI
                     GRGQRELIIGDRQTGKTAIAIDTIINQRDKNVYCIYVAIGQKKSTVAQIIAKLEETDA
                     MKHTIIIVAGADDNASMQYIAPYAGCAISEDFMEKGKDALVIYDDLTKHAWAYRQISL
                     LLRRPPGREAYPGDLFYLHSRLLERSAKLDEKYVIVPKSFAGERADLSDSVDHVLFSG
                     PLAYKDAQTALSQKADKENYSVARLAGSGGSSTALPIIETLLGDVSAYVPTNVISITD
                     GQIYLESGLFNAGIRPAINVGISVSRVGGAAQSDAMKKVSGGLRLDMAAYHEIASFAQ
                     FGSDLDKSTRKQLERGEILQEVLKQPQYSPYSVSDEFLIIYAGTHGYIDGVPLNRVAE
                     WEEEFLKFMSASFPSIGKNIEQTGDLTPEIESDLQKAIEKFKLVWK"
BASE COUNT          841 a          689 c          539 g          921 t
ORIGIN      
        1 cgaccacact tccatttacc tgaattacag ttccagtcgg ttttttcatt taatgctccc
       61 aaacaaaatt tcatcgataa aataaacctt aaccatcgga atgaacttca gagcccccca
      121 ttatatccaa aatatccttc gttatattct cttgccggat tttattgtat tttaactgta
      181 aggattgaag caattcttcc gaattctcag tggcttggtt catggctaac attctcatcg
      241 tatgttcact tgctttagag gatgtcaccg catgaaacaa ttccatccga ataaagcggg
      301 ttataagtga cttcaaaatt acttgaggat ctccttcaaa gatacatccc ccttttcgaa
      361 tgtgtttctc ctctttttca tccaattgtc ctaaaaacgg gtcgacgaac ggaagtaatc
      421 ttttggttgt cggtgtaaaa cgagatatgc tttcatactc ggtataaaca aggtatactt
      481 gcccgactct ttcctgattg aactcgtcga taactatttc ggatacggtt tttaagtcat
      541 gaaagctggt cgcggtcgac aattcgctga aatcagcaat gatatttttt cttcgccttt
      601 gcagcatatc tctgcctttt ttcccgatcg taatatattt cgtcttggtt tgaatttttc
      661 tttccagatg attcacctct cgaaatatgt taaccggata acttcccgcc aacccccgat
      721 cggcgctgat aaagacaatc aaaacatctt tcgaaacatc gggtaattga aaagcgcgaa
      781 tctgattata aaattcctgt tcatggtaca gatcggtcaa tacctgccaa gatagagttg
      841 tataaggtaa agtattttcg gacaatactc taactacacg aacctgactt gcactgacag
      901 cctgtaaagc acgcgttaat tgaccgatgg attcaacggt tcgaatccga ttctgcattt
      961 cacggtttga tgccattttt tatttccaca ccagcttaaa cttctcgatt gctttttgga
     1021 gatcggattc gatttcagga gtcagatcac ctgtttgttc gatatttttg ccgattgaag
     1081 gaaaagatgc gctcataaat tttaaaaact cttcttccca ctcggcaact cgatttaaag
     1141 gcaccccgtc aatatatccg tgtgttccgg cataaataat aagaaattca tcactgacgg
     1201 aataaggaga atattgcggt tgtttgagta cttcctgtaa tatttctccc cgttccagtt
     1261 gttttctcgt tgatttgtcc aaatcggaac cgaattgagc gaaggaggct atttcatgat
     1321 aggctgccat atccaacctc aaaccgccgg aaaccttttt catcgcatcg gattgtgccg
     1381 caccgcctac acgggaaaca gatatcccca cattaatagc gggacgaatg cctgcattaa
     1441 ataatcccga ttcaagataa atttgtccgt cggtaataga aatcacattg gtcggaacat
     1501 aagccgaaac gtctcccaac aacgtttcaa taatgggcaa agcggttgaa cttccgcccg
     1561 atccggctaa tcgagcaacg gaataattct ctttatctgc tttttgtgat aaagctgttt
     1621 gggcatcctt ataagcaaga ggaccggaaa ataatacatg atcgacagaa tcagacagat
     1681 cagcccgttc accggcaaat gatttcggaa cgatcacata tttttcatcc aactttgcgg
     1741 aacgctccaa taatctggaa tgaagataaa aaagatctcc cgggtaagct tctcggccag
     1801 gcggacgcct taataacagc gaaatttggc ggtatgccca ggcatgtttg gtcaagtcat
     1861 cgtaaatgac caacgcatcc tttccttttt ccataaaatc ttcactgatt gcacatccgg
     1921 cataaggggc aatatattgc atcgaagcgt tgtcatcggc gccggcgaca ataataatcg
     1981 tatgcttcat cgcatccgtt tcttcaagtt ttgcgatgat ttgagctacc gtcgatttct
     2041 tctgaccgat tgccacataa atacaataaa cattcttatc ccgttgattg ataatcgtat
     2101 cgattgcgat ggctgttttt ccggtttgac gatccccgat aatcaattct cgctgacccc
     2161 ggccaatggg aatcatggaa tcaatcgctt tgataccggt ttgcaaaggc gtattcacat
     2221 cagatctctc atgaatcccc ggcgcaatcc gttcaatcgc acgatatccg tcgaatcgaa
     2281 tcggaccttt tccgtcaatc ggctcgccaa gcggattaat aacccgcccc aacaacccct
     2341 ccccgaccgg aatcgaagcg atacgacctg tcttttttac aatggaaccc tctttgatgg
     2401 taaagtaatt gcctaaaaca atcactccga ttgtatcttt ttcaagactg aatgcaattc
     2461 ctctggctcc gttttcaaac tcaacgagtt cctgactgcc gattgtgggc aaaccgctta
     2521 cgcgtgcgat tccctcaccg acttccataa cgattccgat atcttccacc tcataattgg
     2581 gttgaaagtc atcgattttt tcaaaaagat cagcggttat atttcggata gtttcttctg
     2641 tcataaatcc tcagaattac atttcgatgt tttattttct ttctcataat tccattttca
     2701 aatgcatttc cttacctcat atattataga gtttctgatc aacgttgaat ccgtcgcact
     2761 gaaaatgtat tacgtcaaag ctgattcgtg agaatctgaa cgagcttatt ttaccatttt
     2821 taaaataatt ccaaaggcga aagaaataat atatattttt ttgaacttta agaatctgtg
     2881 gcggttaccc gaaatcacat taagatattt ttacaccttg tcaaatatgg ttttatcatt
     2941 tagaataaac cacgctagtt aacaaattca caaggaattt aaaatggagt
//
LOCUS       DAOMIV010000246         2990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_35406_length_2990_cov_3.920954, whole genome shotgun sequence.
ACCESSION   DAOMIV010000246 DAOMIV010000000
VERSION     DAOMIV010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35406_length_2990_cov_3.920954"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PKV59_06355"
     CDS             <1..364
                     /locus_tag="PKV59_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQP46411.1"
                     /translation="NILPLTAATKYLMNEERFNQMKKGAYYLNVGRGATTDTQALIEA
                     LQSGKIKGAGLDVIDVEPLPPDSPLWDMDQVIILAHQAGFTTQYDERTLQLFERNLRN
                     YLEKGKPNENLVNLEAGY"
     gene            641..2452
                     /locus_tag="PKV59_06360"
     CDS             641..2452
                     /locus_tag="PKV59_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQP46412.1"
                     /translation="MTEKSKDVYLGMGAIVHEGGVAFRVWAPHADAVFVKGTFNNWSE
                     SADPMKAEEHGTWYTDIPHAKVGDEYKFVLISGNQKLERMDPYARQVTNSAGNCVIYD
                     PKAFDWEGDRFELPPFNELVIYEMHIGSFFADSDKKPGDFDSAAEKMDHLKRLGVNAI
                     QVMPVAEFAGDYSWGYNPANIFAVESAYGGPDGFKKFIREAHQRGFAVIQDVVYNHFG
                     PGDLSLWQFDGWQENGKGGIYFYNDDRSETPWGSTRPDYGRGEVRSYILDNVQMWLEE
                     YHVDGLRFDSTLYIRTIDGSEGNDLPDGWSLLQAINEKIRAYSTHVISIAEDLRSNEW
                     ITKEISENGAGFHAQWDANFVHPVRDAVTVADDSARSMDVIRNAVQFKYENDAFRRVI
                     YSESHDEVANGKARVPQEINPDDPTGVFAQKRSTLAAGLVFTAPGIPMLFQGQEFLEG
                     DYFRDDVPLDWDLNTEFSGIVRLYRDLIRLRSNQKNQTRGLCGQFLNVFHVNEEEKVL
                     AFQRWDQHGAGDDVIAVFNFGNAGKTDYVIGLPTKGIWKLRLNSDAKIYSKDFEGFFS
                     NDTEGFEGGKVGFDFHGSVNIGPYSLLIFSQERNGNH"
     gene            2726..>2990
                     /locus_tag="PKV59_06365"
     CDS             2726..>2990
                     /locus_tag="PKV59_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQP46413.1"
                     /translation="MVERISLFLPFYLGHVNCYLIKTAEEFVLIDTGGTNQRQKLLRQ
                     LASCQCSPGKLRLIILTHGDFDHSGNAAYLREHFGGRIAMSPLD"
BASE COUNT          847 a          548 c          758 g          837 t
ORIGIN      
        1 taatattttg cccctcacgg ctgctacgaa atatctgatg aatgaagaac gctttaatca
       61 aatgaagaaa ggtgcctatt atttgaacgt cgggagggga gctacgacgg atacccaagc
      121 tttgatcgaa gcactgcaaa gcggaaaaat caaaggagcc ggactggatg taatcgatgt
      181 ggagccgcta ccccccgata gtccgctttg ggatatggat caagtaatta ttttggctca
      241 tcaagcaggt tttacgacgc aatacgatga acgaacgctg caactttttg aaagaaacct
      301 gagaaattat ctcgaaaaag gaaagccgaa tgaaaatctg gtcaatctgg aagcgggata
      361 ttaaacggcg agcaatttca taacaaaaac catttcatcc gtttaacctg atcgacgaac
      421 ggatgaaatg atcaaagact ttttcatgcg actgaggtgt gcttaaacga gaaaaatgaa
      481 agagtcaaat cgtttcaatg tttttagctg atcggattga tccgaacttg gcgaaaaaag
      541 cgaaagatcc cggtaaaaga tatatcgatt atctttcggg atctttttta cggattcaaa
      601 aaattaaccg gcaaaaaaat tgcgaatgaa ggatgattaa gtgactgaga aatcaaaaga
      661 tgtttatctt ggaatgggtg cgattgttca tgaaggaggc gtggcttttc gggtctgggc
      721 tccccatgcc gatgctgttt ttgtaaaagg aacttttaat aattggtccg aatccgctga
      781 tccgatgaag gcggaagagc atgggacttg gtatacggat atcccacatg ccaaagtggg
      841 ggatgaatat aaattcgtcc taatcagcgg aaatcaaaaa ttggaacgaa tggatcccta
      901 tgcgcgtcag gtgacaaatt ccgccggaaa ttgtgtcatt tatgatccca aggcttttga
      961 ttgggaaggg gatcgttttg aacttcctcc ttttaatgaa ctggtcattt atgaaatgca
     1021 tatcggatcc tttttcgctg attccgataa aaagcccggt gattttgatt cggcagcgga
     1081 aaagatggac catttaaagc ggttaggggt gaatgccatt caagtcatgc cggttgccga
     1141 atttgccgga gattactctt ggggatacaa tcctgcgaat attttcgccg tagaaagtgc
     1201 ttatggaggt ccggacgggt ttaaaaagtt tattcgtgaa gcgcatcaac gcggattcgc
     1261 agtgattcag gatgtggttt ataaccattt cggacccgga gatttatctc tttggcaatt
     1321 tgacgggtgg caggaaaacg gcaaaggcgg aatctatttt tacaacgatg accgttccga
     1381 aacgccctgg ggaagtacac ggccggatta tggcagagga gaagttcgca gctatatctt
     1441 ggataatgtt caaatgtggt tggaagagta tcacgtcgac ggtttacgct ttgacagtac
     1501 actttatatt cgcacgattg acggttcaga aggaaacgat ttgccggacg gttggtcttt
     1561 actgcaagcc attaacgaaa aaatccgggc atattccacc catgttattt ccatcgccga
     1621 ggatttacga agcaacgaat ggatcaccaa agaaatcagt gaaaacggag ccggttttca
     1681 tgcccaatgg gacgctaatt ttgttcatcc ggttcgcgac gcggtgacgg tggcggatga
     1741 ttccgcccgt tcaatggatg tgatccgaaa tgcggttcag ttcaaatatg aaaacgatgc
     1801 gtttcggcgg gtgatttaca gtgaatccca tgatgaagta gcgaacggaa aagcacgggt
     1861 tccgcaggaa attaatccgg acgatccgac cggtgtgttt gcacaaaaac gttccacgct
     1921 tgctgccggt ttggttttta cggctccggg tattcctatg ttgtttcaag gacaggaatt
     1981 tcttgaaggg gattattttc gggatgatgt gccattggat tgggatttaa acacagaatt
     2041 cagcgggatc gttcgccttt atcgcgattt aatacggtta cgatcgaatc aaaaaaatca
     2101 aactcggggt ttgtgcggac aatttttaaa tgtcttccat gtaaacgagg aggagaaggt
     2161 attggcattt caacgttggg atcaacacgg tgcgggtgat gatgtaatag cggttttcaa
     2221 tttcggaaat gcgggcaaaa ccgattatgt gatcggactg ccgacaaaag ggatttggaa
     2281 gttgcgttta aacagtgacg caaaaatata cagtaaagat tttgaggggt ttttcagcaa
     2341 tgatacggaa ggtttcgaag gcggaaaggt cggatttgac tttcatggat ctgtcaatat
     2401 cggaccttac agtttgttga ttttcagtca agagcggaat gggaatcatt gaatcattat
     2461 cgggaatccg gatgaatcga attttgaatt tgctatgatg ggaatatcac atatatgacg
     2521 cggttttcca caagactgta ttgcccctgt gaattgattc gcagaaacag tcgatcttgt
     2581 ctattttttg atatcgccga taggaacggt ttccgcttaa agcaagctgt tgtttgtagg
     2641 tttcaaatgc tccatcaggc gaaattgaat gtttcgtata gtatgatcgg aaaatagatt
     2701 tatttctttt ggggaacgga tatgaatggt tgagcgtatt tctctctttt tgccctttta
     2761 tctcgggcat gtcaactgtt atctgatcaa aaccgctgaa gaatttgttc taattgatac
     2821 aggcggaacg aatcaacgtc aaaagttgtt gcggcaatta gcttcctgcc aatgttctcc
     2881 cgggaagtta agactgatta tcttaacgca cggtgatttt gatcattcgg ggaatgcggc
     2941 ttatcttcgt gaacattttg gcggcagaat cgccatgagc cctctggacc
//
LOCUS       DAOMIV010000247         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_35500_length_2985_cov_2.997952, whole genome shotgun sequence.
ACCESSION   DAOMIV010000247 DAOMIV010000000
VERSION     DAOMIV010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35500_length_2985_cov_2.997952"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PKV59_06370"
     CDS             <1..969
                     /locus_tag="PKV59_06370"
                     /inference="COORDINATES: protein motif:HMM:NF021006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM1812 family CRISPR-associated protein"
                     /protein_id="HQP46414.1"
                     /translation="EVALDVTNGHRSVSLLGLLAAAFMRTARDIEIRHLLYGAFNIDK
                     TNAPDVSPVFDLSPMLALLNWSVAADRFNRSGDSHDMAELLNDYGKEIKHQAHGNKEE
                     LDRGGAFLKMAKSLNNVTDALYLLRPYDVMENTAYLEKTIRQAIPVLEDSPRSKPLEF
                     LLSRILDTYTPLSASAEELKGHDPIPALRKEQQMIFWYYERDLWVQTISLTREWLISW
                     IMVWFGEKNVLEKSRRECYEKNISLFVWPERKTEKTEWTDADIDRLMPVEQFRKLWQN
                     VTNSRNDIDHAGHRKDRRKSENLKQMIRNCVETIRELPFPTSLEEH"
     gene            966..1346
                     /gene="csx15"
                     /locus_tag="PKV59_06375"
     CDS             966..1346
                     /gene="csx15"
                     /locus_tag="PKV59_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx15"
                     /protein_id="HQP46415.1"
                     /translation="MIILNFSHPLTAAQCEKIEALARQSIDLIYDLPVQFDNDRPFSE
                     QIEALAGTIPLESDRLQSEPILINLPSLNYIAVGLVAWLNGRMGYFPAILRLKGNTDI
                     TPMVYEVAEIINLQSLRDLSRRKR"
     gene            1350..2195
                     /gene="cas6"
                     /locus_tag="PKV59_06380"
     CDS             1350..2195
                     /gene="cas6"
                     /locus_tag="PKV59_06380"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HQP46416.1"
                     /translation="MSVDLLGISLAVSYIGTPESLADRQLPQWWGRATQAAFLQAVRQ
                     ADPELADELHRENTQHPYTVSGLLGWRKGTVPTPEMSFHIRLTGLTAKVNDILQCLTG
                     TGGIFSAGQTIMLDYLPFRIAESAPDADGGRNYSDLIAAGLTRAEVNVLDFRFRSPTL
                     FKSKDKTQPLPLPELVFRSLSERWNTFAPVMFPEELLRFAEECLAISAFNLKSLPVPL
                     KEGVLRIGSIGRVRFRALNADRYWLSLCHTLSLFAGYAGVGSGTGYGLGQAEVPLPKK
                     KEETA"
     gene            2400..>2985
                     /gene="cas1"
                     /locus_tag="PKV59_06385"
     CDS             2400..>2985
                     /gene="cas1"
                     /locus_tag="PKV59_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HQP46417.1"
                     /translation="MAFLSDTCGKKCTYPRLLFQRKEAYIPPIYIVEQGTKIRIHNRC
                     VQIEKENDGAVQVLCRSPIGTVSQIVIFGNIGLTTPAIGLFLEENIDVVFLSVHGEFR
                     GRLATGLSPHIALRKAQYHCLDEPEFVLDLSIQFVRAKLQHQKALLQRHNRDLKFQHR
                     KKRPSPDPINAILSFGYTLLAQSAISAVQVVGLDP"
BASE COUNT          761 a          640 c          760 g          824 t
ORIGIN      
        1 gaggtcgcat tagatgtgac caacggacac cgctccgtgt cattgttggg tcttttggcg
       61 gctgctttca tgcggaccgc ccgtgatatt gagatccggc atttgcttta cggggctttt
      121 aatatcgata aaacaaatgc acccgacgtt tcgccggtct ttgatctgtc gcccatgttg
      181 gctttgctga attggagtgt cgcagccgac cggttcaacc ggtccggtga ttctcacgat
      241 atggcggaat tattaaacga ttacggaaaa gaaatcaaac atcaggcaca tgggaataaa
      301 gaagaattgg atcgcggcgg agcttttttg aaaatggcga aatcgttaaa taatgttacg
      361 gatgctttat atttgttacg cccgtatgat gtgatggaaa atacagctta tttggagaaa
      421 acgattcggc aagcgattcc ggttctggaa gattctccgc gatcaaaacc gcttgaattt
      481 ctcttgagtc gaattttgga tacatacaca ccgctctcgg catcggcgga agagttgaaa
      541 ggtcatgatc cgattccagc tttgagaaaa gagcagcaga tgattttttg gtattatgaa
      601 cgggatttat gggttcagac catttcactc acacgggagt ggctgatcag ttggattatg
      661 gtatggttcg gcgaaaaaaa tgttttggag aaatcccgcc gcgaatgtta tgagaaaaat
      721 atcagtttgt tcgtgtggcc tgagaggaaa acggaaaaaa cggaatggac ggatgcggat
      781 atcgatcgct tgatgcccgt tgaacaattc cggaaacttt ggcagaatgt gacaaacagc
      841 cggaatgata tcgatcatgc cggtcatcgt aaagataggc ggaaatcgga aaacttgaaa
      901 caaatgattc gaaattgtgt cgaaacgatt cgagaattac cttttccgac atcgttggag
      961 gagcattgat tattttgaat ttttctcacc cattaaccgc tgcgcagtgt gaaaagatcg
     1021 aagcccttgc aagacaatcg atcgatttga tttatgattt gcctgttcaa tttgataatg
     1081 atcgcccgtt ttcggagcag attgaagcgc ttgccggaac cattccgtta gaaagcgaca
     1141 gactgcaaag cgaacccatt ttgatcaatt tgccttcttt gaactacatt gccgtggggt
     1201 tagttgcctg gctgaacggg cgaatgggat attttccggc gattctgcgc ttaaaaggaa
     1261 atacggatat tacccccatg gtttatgaag tagctgaaat tataaattta caatcgctgc
     1321 gggatttgtc gcggaggaag cgttaaacaa tgtctgttga tcttctcggt atttcattgg
     1381 ctgtttccta tatcggtact ccggaatcgc tggcggatcg ccagctcccg caatggtggg
     1441 gacgggcaac tcaggcggct tttttgcagg cggttcgcca agcggacccc gagctcgcgg
     1501 atgagttgca ccgggaaaat acgcaacacc cttataccgt ttcaggatta ttgggttggc
     1561 gaaaaggaac cgttccgaca ccggaaatgt cttttcatat tcgcctgacc ggtctgaccg
     1621 cgaaggtcaa tgatatctta cagtgtttga cggggaccgg cggaatattt tccgccgggc
     1681 aaacgatcat gttggattat ttgccgtttc ggattgccga gtcggcgccg gatgcggatg
     1741 gcgggagaaa ctattcggat ctgattgcag ccggattaac ccgtgccgaa gttaatgtct
     1801 tggattttcg gttccgctcg ccgacgttat ttaaaagtaa ggataaaaca caaccgctgc
     1861 cgcttccgga gttggtattc cgttctcttt cggagcgctg gaatacgttc gctccggtta
     1921 tgtttccgga ggagttgtta agatttgccg aagagtgttt ggcgatttcg gcgtttaatt
     1981 taaaatcact tccggtcccc ttgaaggagg gagtattgcg aatcggctcg atcggacgtg
     2041 tccgttttcg ggctttaaac gcggaccgtt attggctcag cctttgtcac accctttctc
     2101 tgtttgccgg atatgccggc gtcggcagcg gaaccggcta cgggttgggg caggcagaag
     2161 tgcctttgcc gaaaaaaaag gaagaaaccg cctaagcggt ctgtaaatcc ttcaaagcac
     2221 cttaacaact gaataattct aaaaacaatc cgtgtatttt catggaggag aactctgctt
     2281 tctcaacgga acacgtccgg agccggtatt cttcctgttg ataacccatg ccgcatcgca
     2341 acgcttaact ctccactaaa aaatatttta tgcggataac tgttcctatc cgaaaggcaa
     2401 tggcgttttt gtctgataca tgcgggaaga aatgtaccta tccccgcttg cttttccaaa
     2461 gaaaggaggc ttatattcca ccgatttata ttgttgaaca aggaacaaaa atacgaattc
     2521 ataatcgttg cgttcaaatt gagaaagaaa atgacggagc ggtacaagtt ctttgtcgca
     2581 gtcccatcgg cacggtttct cagatcgtga tttttggtaa catcggtttg acgactccgg
     2641 caatcgggct ttttctggaa gaaaatattg atgttgtctt tttgagtgtt cacggagaat
     2701 ttcgtggaag gcttgccacc ggactttcgc cgcatatcgc tttgcgaaag gctcaatatc
     2761 attgcctgga tgagcctgag tttgttctcg acttgtcgat acaatttgta cgggcaaaat
     2821 tgcaacatca gaaagcgtta ttacaacgcc ataatcgcga cttgaaattt cagcaccgaa
     2881 aaaaacgacc cagccccgat cccatcaatg cgatcctttc gtttggatat actctgttgg
     2941 ctcagagcgc gatcagcgca gtgcaggtag tcggtttgga tccgt
//
LOCUS       DAOMIV010000248         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_35544_length_2982_cov_3.566109, whole genome shotgun sequence.
ACCESSION   DAOMIV010000248 DAOMIV010000000
VERSION     DAOMIV010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35544_length_2982_cov_3.566109"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKV59_06390"
     CDS             complement(<1..885)
                     /locus_tag="PKV59_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQP46418.1"
                     /translation="MKTKLTLLLVLIFVVAVSPVAAADKPTVTLWSTGSQNVSDLFNN
                     LIEAYNARPESTSVVKLQFILSGTGEDTLSSRIAAAYKTGKTNSGFDIIAENSTSLQQ
                     YIDEAGSTDLFRDLDFSKIPNYKNLLIKPSIYESKFVPYRGTTVVMAYDSARLPNPPK
                     TWDELEAYVQANPGKFAYNSPSTGGAGSGFVRTAIYKFLPEESYMSNDPKWAEQYDQG
                     FALLKRIHPYLYKSGGKVVYPNKNQGTLDLLINKEVDLIPAWADQTLSNLANGTLPDT
                     VKIYQLEDYPPGGTDVVTA"
     gene            complement(971..1984)
                     /locus_tag="PKV59_06395"
     CDS             complement(971..1984)
                     /locus_tag="PKV59_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46419.1"
                     /translation="MSDQVKLEIRNMTKLYKTGDGVRNINLTVNLGELVTLLGPSGCG
                     KTTILRTVAGFLSCDTGSILIDGKDISHLPPEKRPTAMVFQSYNLWPHMTVYENMAFG
                     LKLRKIPKPEIQRRITEMLELVKMSGFEKKYPNQMSGGQQQRIAIARSLLLQPEVLLL
                     DEPFSALDAKIRSQMREELRKIQTDLEITVVFVTHDQEEAMALSDRIVVMNKGSFEQI
                     GTPAEIYDHPASRYIADFIGEMNFIEQRDGTTIAVRPENVTLELGDGTGGDIGGYVRT
                     IMMLGHFYEIAIGTDSGIVKAYVSAETAGKYKRGDMVNCYFEGTYRYEPDSEKKKEKI
                     APA"
     gene            complement(2018..2857)
                     /locus_tag="PKV59_06400"
     CDS             complement(2018..2857)
                     /locus_tag="PKV59_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQP46420.1"
                     /translation="MSFLNPKRWYHSLGKRRFYEALLLIVFILFFYGPLLYTFMLAFA
                     NKYEYPSIIPSQFGFQWWNYVLNQPTLVKSIGLSFEFAFATTFISMIICLPAAYVLAR
                     YNFPGRKMVMFSFLLGNAFPKIGIYTSMAIVFYKMNLMGTFTGVLLIHLIECMMMMIW
                     LPTSAFRNVPANQEEAARDAGAGPLRTFWYVTLPTAWPGIAVAAMFAFIASLGESTGT
                     LLVGLPYYRTMTVEMYGVILDYPSTAGAVFSLILIAPNLALLLLMRRYLGADTLARGY
                     KMG"
BASE COUNT          796 a          694 c          670 g          822 t
ORIGIN      
        1 agctgtcact acatccgtac cgccgggagg ataatcttcg agctgataaa tctttacggt
       61 atccggtagt gttccgtttg ccaagttgct caaagtttga tccgcccaag cggggatcaa
      121 atccacctct ttgtttatga gcaggtcaag cgttccctgg ttcttgttgg gatagaccac
      181 tttcccgccc gatttataca gataaggatg aattctcttc aacaaagcga aaccttggtc
      241 atattgctcc gcccatttcg gatcattcga catataggat tcttccggca ggaatttata
      301 aatcgccgtg cgaacaaagc ccgaacccgc gccgccggtg ctcggagagt tgtaggcaaa
      361 tttcccgggg tttgcttgaa catatgcttc caattcgtcc catgttttgg gaggattggg
      421 gagtcgggcc gaatcataag ccataaccac tgttgttccg cggtacggta caaatttgct
      481 ctcataaatg gaaggtttaa tcagaagatt cttataattg gggatctttg agaagtcaag
      541 gtcccggaat aaatccgtac ttccggcttc atcgatatat tgttgcagag aggtactgtt
      601 ttccgcgata atatcaaaac cggaattggt cttacccgtc ttataggctg cggcaatccg
      661 tgaacttaat gtatcttcac cggtcccgga aaggataaat tgcagtttta cgacagaggt
      721 tgattcgggt cgagcgttat aagcctcaat aagattattg aagagatcgg aaacattttg
      781 tgatcccgtg ctccataaag ttacggtcgg cttgtccgca gccgcgaccg ggctgacagc
      841 gactacgaaa atcaacacaa gcaataaagt taattttgtt ttcattaaaa tactcctttt
      901 ttgattaatt ccggctccca aaattgggaa gcgcggttat tcaacgaatg ataaatttgc
      961 accggcagga tcatgcgggc gcaatttttt cctttttctt ttccgaatcg ggttcgtaac
     1021 gataagtgcc ttcaaaataa caatttacca tgtctccgcg tttatatttt ccggctgttt
     1081 ccgccgatac gtacgctttc acaattccgc tgtccgttcc gattgcaatt tcgtagaaat
     1141 ggcctaacat cataatggtt cgaacgtaac cgccgatatc tccgccggtt ccgtccccca
     1201 attccagtgt cacgttttcg ggacgaacgg cgattgttgt tccgtcacgc tgttcgataa
     1261 aattcatttc tccgatgaag tcggcgatat agcgggatgc cggatgatcg taaatttccg
     1321 ccggggttcc gatctgttcg aaagatccct tattcatcac cacaatccga tcggacagcg
     1381 ccatcgcctc ttcctggtca tgcgtgacaa agacaaccgt gatttccaaa tcggtctgga
     1441 tcttccttaa ttcctcacgc atttgagaac ggattttggc gtccaatgcc gaaaaaggtt
     1501 catccaagag cagcacttcc ggttggagca aaagagaacg ggcaatcgcg atccgttgtt
     1561 gttgtcctcc cgacatttga ttgggatatt tcttttcaaa gcccgacatt tttaccaatt
     1621 cgagcatttc ggtaattctt ctttggattt ccggcttcgg aattttccgc aatttcaagc
     1681 cgaaagccat attttcataa acggtcatat gcggccataa gttatagctt tgaaaaacca
     1741 tagcggtagg gcgtttttcg ggcggcaagt gagaaatatc cttaccgtcg atcaaaatcg
     1801 aaccggtatc gcaactcaaa aaaccggcaa ctgtgcgtaa aatggttgtt tttccgcaac
     1861 cggacgggcc gagcaaagtg actaattctc ccagatttac agtcagatta atgtttctga
     1921 ctccgtcgcc cgtcttatat agtttggtca tattgcggat ttcgagtttt acctgatcac
     1981 tcataatgta aagtttcctt cccggttttt tgatgaatta acccattttg tatccgcggg
     2041 cgagcgtatc cgcgccaaga tacctgcgca tcaataataa caatgcgagg ttgggggcga
     2101 taaggatcaa agagaaaaca gcgccggcgg tagatgggta atcaagaata acgccataca
     2161 tttctacggt catcgtgcgg taatacggca aacccaccaa gagagtgccg gtgctttcgc
     2221 ctaaagaggc gataaaagca aacatagcgg cgacggcgat cccgggccag gcagtcggca
     2281 gcgttacgta ccaaaaagta cgcagcggac cggcaccggc atccctggct gcctcctctt
     2341 ggttggcggg aacattgcgg aatgcgctgg tcggaagcca aatcatcatc atcatgcatt
     2401 cgatcagatg gatcagcaga acacctgtaa acgtccccat cagattcatc ttgtaaaaca
     2461 cgatcgccat ggaagtgtag ataccgattt tcgggaatgc atttcccaac aggaaagaaa
     2521 acatcaccat tttccttccg ggaaagttat atctcgcaag aacgtaagct gcgggaaggc
     2581 agatgatcat tgaaataaac gttgtcgcaa acgcaaactc aaacgaaaga ccgattgatt
     2641 tcactaatgt cggctgattc agtacataat tccaccattg aaagccgaat tgagagggaa
     2701 taatcgaagg gtactcgtat ttatttgcga acgccagcat aaaagtgtaa agcaacggtc
     2761 cataaaagaa caggataaaa acaatcagaa gaagtgcttc ataaaaacgt cttttaccga
     2821 gactgtgata ccaacgttta gggttcagga aactcatttt tccgattatc tcctttctgt
     2881 ctggcttttt gttcgataaa accaagaaaa tctattttga tctttatggc catttttacc
     2941 ttttttgtgg tgatatttcg tactcttaat cgtagcagcc tg
//
LOCUS       DAOMIV010000249         2971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_35729_length_2971_cov_4.337449, whole genome shotgun sequence.
ACCESSION   DAOMIV010000249 DAOMIV010000000
VERSION     DAOMIV010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35729_length_2971_cov_4.337449"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..1856)
                     /locus_tag="PKV59_06405"
     CDS             complement(237..1856)
                     /locus_tag="PKV59_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQP46421.1"
                     /translation="MNIPSADQILEFDDLKYPQDFLDQYELLECIAQKTESETFFVKN
                     RQSGEFAVAKCYIGKELISEPTEGQILNLLHHNGLPRFIEEYKNDETICVVREYIQGI
                     SLSERMKDGSLTERDAIPILLQLCDILTYLHHQCPPIIHRDIKPENIILAEDGSVKLI
                     DFGIARTYDKSEKKDTIIFGTQGYAPPEQYGFSQTDCRSDLFSLGIIIGYLLTGKTDR
                     EAVAKTLTNKRILHIYQKCTDFSPKRRYSSATNLKRALLRSDGRRQKTFSRFAKIALF
                     SVLFFGLGLIAGRFTDFPAFPAAEVTFDEPLIEKAVRLQIDRADDLPITKDDLLSVTE
                     LYIFGDNVVAKTEDEMNRSAELLFQEGRMEPGSIVSLRDLNKLPNLRKVAVSMQRIKD
                     LTPLANLKQLQILILKNNPIEDLSPLNGLDSLERLSVFNTSVKDFSPLAECIRLRDLD
                     AGDTLVRTPIAFAPAKNLTHLNLYKLQLDTLAGVELLRNLEFIEISDVADDDLSPLLS
                     LKKLKTVKLSEKLRQAAEAIANAAQFEIVYR"
     gene            complement(1837..2310)
                     /locus_tag="PKV59_06410"
     CDS             complement(1837..2310)
                     /locus_tag="PKV59_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46422.1"
                     /translation="MEKEDRFENGIPTSSLLNRLFKSSDLNDFLKENEKEMVIPTFME
                     HLDRLCRERNVIREHVINRSGIERCFGHQLFRGTRKPSRDNVLRLAFGFGLNVEETQE
                     LLRSAKKAPLYPRIKRDAAIIFGLSHQQTIMEIQSVLNDLELSLLGEEKYEHTER"
BASE COUNT          835 a          627 c          604 g          905 t
ORIGIN      
        1 caatgcttca gcttgactct ccacatcgac agaagattca gcgaatgccg aaaccgttat
       61 accgagacat actataaata tgaagataat gatgagcgac tttgcaattc ttttttccat
      121 ttttcagacc tctttgttcg gtaaaattga catattgtca ttgacagttt cagtctaaca
      181 aaggagaaaa taaaattggt gaacaaattg ctcacttatt tggcttgatt gatctctcag
      241 cgataaacaa tttcgaattg tgccgcattt gcgatcgctt ccgcggcttg acgtagcttc
      301 tcgcttaatt tgactgtttt caactttttc agggataaaa gaggggaaag atcgtcatcg
      361 gcaacatcgc tgatctcgat aaattccaga ttccgaagca attcgacccc cgccaatgta
      421 tccagctgta acttataaag atttagatgg gtcaggtttt tcgccggcgc aaaggcgatt
      481 ggggtccgaa caagcgtgtc tccggcatcc agatctctca agcgaataca ttccgcaagc
      541 ggagaaaaat ctttgacaga ggtattaaac acggacaacc tttccaaact atccagccca
      601 tttaatggag agagatcttc tatcggattg tttttcaaaa taagaatttg gagttgtttt
      661 agatttgcga gcggggtcaa atctttaatc cgctgcattg aaacagccac ttttcttaag
      721 ttgggcaatt tatttaaatc tctaagggac actatcgaac cgggctccat ccggccttcc
      781 tgaaagagca gttccgctga ccggttcatt tcatcttctg ttttcgcgac tacgttgtcg
      841 ccgaaaatat acaattcggt aacagaaagc aagtcatctt ttgtaatggg caggtcatcc
      901 gctctgtcga tctgaagacg aaccgctttt tctatcagcg gttcgtcgaa tgtcacttca
      961 gcagccggaa aggcaggaaa atcggtgaat cgacccgcaa tcaaccctaa accgaaaaac
     1021 aaaacagaaa acaaagcgat tttagcaaaa cgggaaaaag ttttttgcct tctaccgtcc
     1081 gagcgaagaa gagcccgttt aagattagtc gccgaggagt aacgcctttt gggtgaaaaa
     1141 tccgtacatt tttgatagat atgcaaaatc ctcttgtttg ttagagtttt tgctacagct
     1201 tcgcgatccg tttttccggt taataaatac ccgatgatga ttcccagcga aaacaaatcg
     1261 gaccggcaat ctgtttgtga gaaaccgtac tgctccggag gagcataacc ctgtgttccg
     1321 aaaatgatcg tatccttttt ttcggatttg tcataagtcc gtgcgatacc aaagtcgatc
     1381 agcttgacgg aaccgtcttc ggcaagaata atattttcag gctttatatc tctatgaata
     1441 attgggggac attgatgatg caagtatgtc aaaatatcgc agagctgtaa aagaatcgga
     1501 atcgcatccc gttccgtgag cgaaccgtcc ttcattcgct cagacagaga gatcccttgt
     1561 atatattcgc ggacaacgca tattgtctca tcatttttat attcttcgat gaatcggggg
     1621 agaccgttat ggtgaagcaa atttaatatt tgaccttcgg tcggttcgga tatcaattct
     1681 ttcccgatat agcattttgc gacggcgaat tctccggatt gtctgttttt tacaaaaaaa
     1741 gtttcacttt ccgttttttg tgcgatacat tccagtagct catattgatc aagaaaatcc
     1801 tgcggatatt ttaaatcatc gaattcgagg atctgatcag cgctcggtat gttcatattt
     1861 ttcttcacct aacaacgaaa gctccaaatc attcaataca gactgtattt ccataattgt
     1921 ctgttggtga gaaagtccga aaataatagc ggcatcccgt ttgattcgag gataaagggg
     1981 agcttttttg gccgagcgca aaagttcctg tgtttcttca acattcagcc caaaaccaaa
     2041 ggctaaacga agcacattat cccgggaagg cttcctggta ccgcgaaaga gttgatgccc
     2101 gaaacaacgt tcaattccgg aacgattgat tacatgctct ctgatgacat ttcgttcccg
     2161 gcaaagtctg tcgagatgtt ccataaatgt cgggataacc atctcttttt cgttttcttt
     2221 gaggaaatca tttaaatcgg atgacttaaa aagtctgttt agtaaagaac ttgtcggtat
     2281 tccgttttca aaccggtctt ctttttccat tttcatttcc tctggcacat ctttttcgcc
     2341 ggatctgtgt caagaatgtg atgcatcgcc ataagagcag cgaaacgtca aaagcaccat
     2401 aaaatataga aattaaccat aaacaatgag acattatttt ggatcgggga aagggaagtc
     2461 tttgatggta gcatcattgc cgcgataatt tgattgaaag gcattccttc gggtctcatt
     2521 tcggttaaaa aagtaaattc ccgagcaatt gaatgttgaa tccgtttaaa atgattattt
     2581 ttccgtaaac tgatttcgaa ttgtatcact atatcgaatt tcatcaccgg tttcccaaat
     2641 accggcctta ttttgctttc ctgtgccgaa tatagaattc ctttcgtgaa accggcgaac
     2701 agtttctatt ctaaggatga atcttgtggt tgaaagcatc tgatattccc ggcataattt
     2761 gctcatcaca ggaatctttt tcgttttttc ggatctcttt attatttctt gcggcatttt
     2821 cttcccgttt cacggaaact caggatttac ggcttcgaac agcaaaggat tttcaatatt
     2881 ttcctatcct gtaagtggtt cggaatgctt caattaccgg cgactgctgc aataaaaaaa
     2941 tattctaccg gcaaggagct tagggtcgaa a
//
LOCUS       DAOMIV010000250         2953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_36056_length_2953_cov_4.288130, whole genome shotgun sequence.
ACCESSION   DAOMIV010000250 DAOMIV010000000
VERSION     DAOMIV010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36056_length_2953_cov_4.288130"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..2388
                     /gene="topA"
                     /locus_tag="PKV59_06415"
     CDS             4..2388
                     /gene="topA"
                     /locus_tag="PKV59_06415"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQP46423.1"
                     /translation="MEAYCLKCKQKREIQNPIAEYTANGRPITKGTCPVCNSKLSLIG
                     KTAAHEGLEKPQITKAAKKANSKKVVAKTHSKDPNPKKINGSKNGTNTTRGIGQKLVI
                     VESPAKARTVGNFLGKGYIVKASIGHVRDLKKSTLSVDVENGFTPEYRVPNEKRALVK
                     ELSTLAKNSDEVYLATDPDREGEAIAWHLLEAADIDPKKTERVVFHEITKPAIAEAFK
                     NPRDINMDLVDAQQARRILDRLVGYNLSPLLWNKVQRRLSAGRVQSVAVKLIVDRERE
                     IENFVPREYWTITVELQPQDIKGTFLSRLVKIDQADPELNNEEQVQFHLNHLQSADYV
                     IDAIKKNVRHRKPSAPFTTSTLQQEASKRLGLTANRTMALAQQLYEGIDLGSEGSAGL
                     ITYMRTDSTNISEIAQNEARKYIQQTYGSGFVPKTAPVYKTKTQRAQEAHEAIRPTTV
                     NRTPEQMKPYLNRDLFRLYQLIWQRFVSSQMEDALYDTLTIDVSAKDAHDYLFRSSGS
                     KLKFQGFLAVYEESKDEDLVVDEMENTKFPEGLAVGQKLQCIHTIPEQHFTQPPARYT
                     EATLVKTLEENGIGRPSTYASILSTIQTRGYVVREAKRLYPTETGILVNDLLVTSFPN
                     VVNIGFTSQMEGELDDVAGGEKKWQTVVGDFYKEFEPALNQARKELPKAQLEPEKVGR
                     KCPQCGKDLVIRNGKYGKFISCSGFPQCRYTEQIVEKLDVPCPKCGKELIVRRSKNGR
                     IFYGCSGYPECQFTSWKKPVKQKCPNCGGMLVEMNRDTLQCIECKTKVENKDSE"
     gene            2489..>2953
                     /locus_tag="PKV59_06420"
     CDS             2489..>2953
                     /locus_tag="PKV59_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46424.1"
                     /translation="MKEKAKAFVEQNPFWTGIIAGFLICFALIGLLSGIGAEQTNSFK
                     NADQILKEDYLRMTVEDYAFTKNADRAKWRYKQLGEEAPSLIALLKKDDLTDPLALFE
                     FSRVVGDMTLFYEGSEIVPEQTNQPISQEDSGGVSLIGKIFILLILLILVGAI"
BASE COUNT          926 a          584 c          708 g          735 t
ORIGIN      
        1 agtttggaag cttattgcct gaaatgtaaa caaaaaagag aaattcaaaa tccgatcgcg
       61 gaatataccg caaacgggag gcctatcacg aagggaacct gccctgtttg caattcaaaa
      121 ctcagtctaa tcgggaaaac agcggctcat gaggggttgg aaaagcctca aataacgaaa
      181 gctgccaaaa aagcaaattc aaaaaaagtt gttgctaaaa ctcattcgaa agatccaaac
      241 cctaaaaaaa taaacggttc gaaaaacgga acaaatacaa cccgaggaat cgggcagaag
      301 ctggttatcg tggaatcgcc ggcgaaagcg cgaaccgtcg gaaactttct gggaaaagga
      361 tatattgtaa aagcttcgat cggtcatgtc cgtgatctaa aaaaatcaac tttatccgtt
      421 gatgtggaaa atggctttac accggaatat cgagttccca atgaaaagcg agctttggta
      481 aaggaattat caacgctggc aaaaaacagc gatgaagttt atctagcaac agatcctgat
      541 cgggaaggag aagcgatcgc ctggcactta ttggaggctg cggatattga tccgaaaaaa
      601 acggaaagag tcgtctttca tgaaattaca aaaccggcga ttgcggaagc ttttaagaat
      661 ccacgcgata ttaatatgga tctggttgat gcccaacagg ctcgtcgaat tttggacaga
      721 ttggtcggtt acaatttaag tcctttactc tggaataagg ttcaacgtcg tttatccgct
      781 gggcgtgttc aatcggttgc ggtaaagcta attgtcgatc gggaacggga aattgaaaat
      841 tttgttcctc gtgaatattg gacgataacc gttgaacttc aaccccaaga tattaaaggc
      901 actttccttt ccagattagt taagatcgat caagccgatc cggaattaaa taatgaagag
      961 caggttcagt ttcatcttaa tcatcttcaa tccgccgatt atgtgatcga tgcgattaag
     1021 aagaatgttc ggcaccggaa accatccgcg ccgtttacaa cttcaactct gcaacaggaa
     1081 gcgtctaaaa gattgggatt aactgccaat cggaccatgg ctctggcgca acagttatac
     1141 gagggcattg atctcgggag cgaaggatcc gccggtttga taacctatat gagaaccgat
     1201 tcaaccaaca tttccgaaat cgcccaaaat gaagcacgga aatacattca gcagacctat
     1261 ggttcaggct ttgttcccaa aacagcgccg gtttataaaa caaaaacaca acgggcacag
     1321 gaagctcatg aggcaattcg tccgaccact gtaaaccgaa ccccggaaca aatgaagcct
     1381 tatctcaatc gggatctttt tcggctgtat cagctgattt ggcagcgttt tgtatcctct
     1441 cagatggagg atgctttgta tgatacactg acgattgatg tcagcgccaa agatgcccat
     1501 gattatcttt tccgttcctc gggatcaaag ctgaaattcc aaggatttct ggctgtttat
     1561 gaggaaagca aagatgagga tttggtagta gatgaaatgg aaaacaccaa atttcccgaa
     1621 gggttagctg tcgggcagaa actgcaatgt attcatacaa ttcccgaaca acattttacg
     1681 caacctccgg cacgatatac cgaagcgact ttggtgaaga cacttgaaga gaacggtatc
     1741 ggtcgaccgt ccacttatgc ctcgatcctt tcgacgattc aaacgcgagg ttatgtcgtt
     1801 cgcgaggcga agcgtcttta tccgaccgaa acggggattt tggtcaacga tctgctcgta
     1861 accagttttc cgaatgttgt taacatcggt tttacctctc agatggaagg tgaattggat
     1921 gatgtagccg gtggggagaa aaaatggcaa acggtggtgg gagattttta taaggaattt
     1981 gagccggctt tgaatcaagc gcggaaagaa ttgccaaaag cacagttgga accggagaaa
     2041 gtcggtcgga aatgcccgca atgcggaaaa gacttggtta ttcgaaacgg caaatacgga
     2101 aaatttatct cttgcagcgg gtttccgcaa tgccgttata cggaacagat agtggagaaa
     2161 ttggatgttc cgtgtccgaa atgcggaaaa gagctgattg ttcgtcgctc taaaaacgga
     2221 aggattttct atggatgcag cggctatccg gaatgtcaat ttacctcttg gaaaaagccg
     2281 gtaaaacaga aatgtccgaa ttgcggcggg atgttggtgg agatgaatcg ggatacgcta
     2341 caatgcatag aatgcaagac gaaagtcgaa aataaagatt ccgaataaaa acgatcgaat
     2401 ttggaaatat aattgggata acgaatcgtt gcagagcttt gtaaataaaa acgcttaaga
     2461 acccattcag ttctatgtgg aggtcccgat gaaagaaaaa gccaaggcat ttgttgagca
     2521 aaatccattt tggacgggaa tcattgccgg atttctgatt tgttttgctc tgatcggact
     2581 gctcagcgga atcggagctg agcaaacaaa tagctttaag aatgccgacc aaattctcaa
     2641 agaagattat cttcggatga cggttgaaga ttatgccttc acaaaaaacg ccgatcgggc
     2701 taagtggaga tacaaacaac tcggagagga agctccctct ctgattgcat tactcaaaaa
     2761 agatgatttg acagatccgt tggctttatt tgaattttcc cgagtcgtcg gggatatgac
     2821 acttttttat gaagggtccg aaattgttcc ggaacagaca aatcaaccga ttagccaaga
     2881 agattccggc ggcgtctcat taatcggaaa aattttcatc ttattgatct tattgatttt
     2941 ggttggagcg att
//
LOCUS       DAOMIV010000251         2953 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_36057_length_2953_cov_4.163906, whole genome shotgun sequence.
ACCESSION   DAOMIV010000251 DAOMIV010000000
VERSION     DAOMIV010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36057_length_2953_cov_4.163906"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKV59_06425"
     CDS             <1..346
                     /locus_tag="PKV59_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46425.1"
                     /translation="LFSQKYAHLPENFMVLFFFSDCLKQFSQYQQESITVDRIVKFEI
                     AQVVIVWSLLKIQAVNKALQIGSLTVDSFLQIAAETDRDFLHYPAYIRQCAQRLSMKF
                     DSNEKLIECLFY"
     gene            complement(340..1581)
                     /locus_tag="PKV59_06430"
     CDS             complement(340..1581)
                     /locus_tag="PKV59_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46426.1"
                     /translation="MGKNSEKSEYRVLLFDIDGVLVSQGGYRKAFTDTVSWFMNYFGF
                     PEIQIGREFADRFDAYGVLAEWDMVPLTLSFFLDWYAENNPQARPFGKLEKLNNLPRI
                     HPAMPFPEVLWQKIPQIQDILNGEDIPALAVYKHCLDQKDQSILPRLWDDSVLSELLA
                     DSLNVRTSWCFQKLETYILGSELFEKTFHIPTEKGIPSALMTVNKALLNEKYRKKILE
                     MNGKSLYVATITARPNLLPACIKDDKTEIISAIPEAEIAMDELGWGDERIKNVGVGIL
                     HYYEKKRDLPQDSRLKPNPFHALFAILYAISDSFEKTFNVVSTWDFPEKSENKINPFL
                     PILPEGSMLDIAVFEDSLSGIQSATDAAKILQNYGYIAQTRRYGIYTNPEKAELMKTI
                     GTKCFPTINDALDQYFSETDQ"
     gene            complement(1581..2759)
                     /locus_tag="PKV59_06435"
     CDS             complement(1581..2759)
                     /locus_tag="PKV59_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQP46427.1"
                     /translation="MDIFQKCYEYDMIKKMIAEGHYPYFLPLSENEGTVAEYKGKKLI
                     MCGSNNYLGLTTHPKVREAAIAAIHQYGTSCTGSRFLNGSLELHETLEHEIAEFVGKE
                     AALVFSTGMQTNLGTISALVGRNDYAILDKDSHASIYDGVKLSYGKLERFAHNDMEHL
                     EKVLKSLPEEAGKLIIVDGLFSMEGDLAPLDKIIPIARKYGARLMVDDAHGIGVTGNG
                     HGTAAQFGLTKEVDLIMSTFSKSFASLGGFISGDEEIVHYIKHNARSMIFSASIQPAS
                     AAAALAALRIMREEPEWNQRLIKNGNKMRKGLSELGFNIGNSVTPVVPILIGNNEQTF
                     EMASGLFERGVFVNSVISPATPPGRQLLRTSYMATHTDEQLDQVLDAFEYVGKKVGLI
                     "
BASE COUNT          865 a          665 c          537 g          886 t
ORIGIN      
        1 acttttttcc caaaaatatg ctcatttgcc ggaaaatttc atggttttat tctttttttc
       61 ggattgtttg aaacaatttt cccaatatca gcaagagtcg ataaccgtcg accggattgt
      121 taaattcgaa attgcccaag tagtgattgt ctggagcctt ttaaaaattc aggcagtaaa
      181 taaagcctta caaatcggat cattgaccgt cgattcgttc ttgcagatcg cagcggaaac
      241 agatcgcgat tttttgcatt atccggctta tattcgacaa tgtgctcagc gactctcaat
      301 gaaatttgat tcgaatgaga aattaattga atgtttattt tattgatctg tttccgaaaa
      361 atattgatcc aaagcatcat tgattgtcgg aaaacacttt gtaccgatcg ttttcatcaa
      421 ttctgccttt tcaggatttg tatagatgcc atatcttctg gtttgggcga tatacccgta
      481 attttgtaag atttttgccg catctgttgc ggattggata ccggatagag aatcttcaaa
      541 aaccgcgata tcaagcatag aaccttccgg aagaataggc aaaaaaggat tgattttatt
      601 ctcggatttt tcgggaaagt cccaggttga aacgacgttg aaagtttttt caaaagaatc
      661 actgatggca taaaggatcg caaaaagtgc atgaaaagga ttcggtttca atctgctgtc
      721 ttgcggaaga tcccgcttct tctcataata atgtaatatc cctactccga catttttgat
      781 ccgctcatct ccccaaccta attcatccat cgcaatttcc gcttcgggga ttgccgagat
      841 aatctctgtt ttatcgtctt taatacatgc cggcaacaaa ttcggacgag cagtaatcgt
      901 cgccacgtac aatgatttcc cgttcatttc gagaattttt ttccggtatt tttcgttaag
      961 aagggcttta ttcacagtca ttaaagcgga aggaattccc ttttcggtcg gtatatgaaa
     1021 tgttttttca aaaagctccg aaccgagaat ataggtttcc aatttctgaa agcaccaaga
     1081 agtacgcaca tttaaagaat ccgccaacag ctcacttaaa accgaatcat cccatagtct
     1141 gggtaaaata ctctgatctt tctgatccag acaatgtttg taaacagcca aagcggggat
     1201 atcttcacca tttaaaatat cttgaatctg cggtatcttc tgccaaagaa cttccggaaa
     1261 cggcatggcc ggatggatgc ggggaagatt atttaatttt tcaagttttc caaaaggtct
     1321 tgcttgcggg ttattctcgg cataccaatc taaaaaaaag gataaagtca acggaaccat
     1381 atcccattct gccaaaacgc cgtatgcatc aaatcgatcc gcaaattccc tgccgatttg
     1441 aatttccgga aatccaaaat aattcataaa ccaggaaacc gtatctgtaa atgctttccg
     1501 atatccgccc tgagaaacca acacaccgtc tatatcaaat aataagaccc gatattcgga
     1561 tttctctgaa ttttttccca tcagatcaag ccaacctttt tcccgacata ttcaaaagcg
     1621 tccaatacct gatcgagctg ttcgtccgta tgggttgcca tgtaactggt tctcaacaat
     1681 tgtctaccgg gaggggttgc gggagaaatt accgaattaa cgaaaacgcc cctttcaaaa
     1741 aggccgcttg ccatttcaaa agtctgttca ttattcccga taagaatcgg tacgaccggc
     1801 gttacggaat tcccgatatt aaatccaagc tcggacagtc ccttccgcat cttatttcca
     1861 ttctttatca aacgctgatt ccattcgggt tcttctcgca tgattcttaa tgcagccaaa
     1921 gctgccgcag cacttgccgg ctgaatactt gcactgaaaa tcatcgatcg ggcgttatgc
     1981 ttaatgtaat gaacaatctc ttcatcgccg gaaataaaac cgcccaaaga agcgaaactc
     2041 ttactgaaag ttgacatgat caagtcgacc tctttcgtca agccgaattg tgcagcagta
     2101 ccatgaccat tacctgtcac accaatcccg tgagcatcgt ccaccatcag acgggcgcca
     2161 tattttcttg cgatgggaat aattttgtcc aacggagcta aatctccttc catactgaac
     2221 aaaccatcca caataatgag ctttccggcc tcttccggga gagattttaa gaccttttcc
     2281 aaatgttcca tatcattatg cgcaaatcgt tccaatttac cgtaggaaag cttgacgccg
     2341 tcataaatgc tggcatggga atctttatcg agaatggcat aatcattccg accgactaat
     2401 gcgctgattg ttcctaaatt cgtctgcatt ccggttgaaa aaaccaaagc cgcttctttg
     2461 cccacaaact cggcaatttc atgttccaaa gtttcatgca attccaacga accatttaaa
     2521 aatcgagagc cggtacaact ggttccgtat tggtgaatcg ccgctatcgc cgcttcccga
     2581 actttcggat gagtcgtgag ccccaagtaa ttattggaac cacacatgat cagtttcttg
     2641 cccttatatt ccgcaacggt tccttcattt tctgaaagcg gcaaaaaata aggataatgt
     2701 ccttcggcaa tcattttttt gatcatgtca tattcgtaac acttttggaa aatatccata
     2761 aaatcctctt attttcttcg ataaattgat gatgattaca cagaaaaagt cttgaaaatt
     2821 ataaatgatg tttccaaaaa tttcggaaat tacactttaa cagattcttg atttacctga
     2881 ttgaaaaaat cagagcgaat cgattcgtct ttcagaaata aaccgctgaa atttgtcgtt
     2941 ataagcttcg cct
//
LOCUS       DAOMIV010000252         2921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_36633_length_2921_cov_3.579902, whole genome shotgun sequence.
ACCESSION   DAOMIV010000252 DAOMIV010000000
VERSION     DAOMIV010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36633_length_2921_cov_3.579902"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="PKV59_06440"
     CDS             <1..322
                     /locus_tag="PKV59_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HQP46428.1"
                     /translation="PLGFGNWQAITSLVSGFMAKESIISTMAVVYKAADQGLQTVLQS
                     IFTPASAFSFLIFVLLYPPCIASVSTMNKEVGTKWTGFAIILELATAWIVSFLAYHIA
                     LLIL"
     gene            329..535
                     /locus_tag="PKV59_06445"
     CDS             329..535
                     /locus_tag="PKV59_06445"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HQP46429.1"
                     /translation="MADWIVGFIVVLLIGWAIYRIIQRRKNPLGNCGGSHSIYGIPDS
                     EIPSYCSNCRARELCRRQAPKARK"
     gene            complement(564..2045)
                     /locus_tag="PKV59_06450"
     CDS             complement(564..2045)
                     /locus_tag="PKV59_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQP46430.1"
                     /translation="MGKIIPELFREIIQNASIKFQTPAISVAVIEDGEISFAEGFGSR
                     TFGKNEEVTADTSYAIASMSKSTVSTSLAILREQNLFGWDDKVTKFLPDFRMADLFAG
                     QEMTVLDLLIHHCGLNSESAGTLWYGSDYSREEVVRRVRYLRPVTGFRSTYAYQNVCY
                     LIAGLIIEKICGMSWDDFICKYIFSPLEMERSFPNLKALQLSGMDNVATPHTQLNGKL
                     SVIPYRNHDNVGPAASVHSTALDLAHYLQMFLNHGVYRGKVILQPESVENLHSMHIVV
                     PPAPAKDLIHPRLLTDFSGYGLGWFIQDYQGKKRVGHSGGVDGMRGRMEIFPALNRSI
                     VILTNSEDRRAYQSILYSAFDMFLESEPIDWINVWENDPEMKQTVTEPERIPNTSPSL
                     PDAAYTGVYEDSAYGEIIVRKEAHRLILQFSHTPAFTADLEHWHFDTFRLIWHDPYIP
                     RGLVTFSLNCEGKVESMHIDQPRLLDVDFNELDYDIRKKPERD"
     gene            2206..>2921
                     /gene="rsmG"
                     /locus_tag="PKV59_06455"
     CDS             2206..>2921
                     /gene="rsmG"
                     /locus_tag="PKV59_06455"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQP46431.1"
                     /translation="MQHMSKWIDIAAQFIGFPLSEDRLRLFEIYESELIEWNTRFNLT
                     AIKNPDEIRTKHFLDSLSCALAINDFNGRSLVDVGTGAGFPGIPLKIVFPDLKLTLVD
                     SVGKKLVFCQHIIDRLSLTDCRIIHARAEDLGKDPLHRERYDIAVARAVTGLSALSEL
                     LLPLVKRKGIMIAQKGETAAEELKAAEKAIRILGGGETQLIPVTIPNAVEQRYLIRIE
                     KQRNTPLGYPRRPDQIRKKP"
BASE COUNT          779 a          646 c          693 g          803 t
ORIGIN      
        1 gcccttaggt tttggaaatt ggcaggcaat cacttccttg gtatccggat ttatggcgaa
       61 ggaaagcatc atcagtacaa tggcggtggt ttataaagcg gccgatcagg gactgcaaac
      121 ggttcttcaa tcgatattta ctccggcatc cgccttcagt ttcctgattt tcgtgcttct
      181 ttatccgccc tgtatcgctt ctgtctcgac catgaataag gaagtgggaa caaagtggac
      241 cgggtttgcg atcattctcg aattggcaac cgcttggatc gtatctttcc tggcgtatca
      301 tattgcttta ttgattttat gagattctat ggctgattgg attgtcggat ttatcgttgt
      361 tttgctcatt ggttgggcaa tttaccgtat cattcaaaga aggaaaaacc cgctcgggaa
      421 ctgcggcgga agccattcga tttacggaat tccggattcc gaaattccgt cgtattgcag
      481 caattgccgg gctcgtgagt tgtgccgcag acaggcaccg aaagcacgca aataattgtc
      541 gcattgtatt tggttgatcg tcgtcagtct cgttctggtt tctttcgaat gtcataatcc
      601 agctcgttaa aatcgacatc gagcaatcgc ggttgatcga tgtgcatcga ttcgactttt
      661 ccttcacagt tcagtgaaaa agtgaccaat cctcggggaa tatatggatc atgccaaatc
      721 aagcggaagg tatcaaaatg ccaatgttca agatctgcgg taaaagcggg tgtatgactg
      781 aattgtagga tcagcctatg ggcttctttt ctgacgatta tttcaccata tgcggaatct
      841 tcataaacac cggtataggc ggcatcaggt aaggaaggag aagtgttcgg aatccgttcc
      901 ggttccgtta ctgtttgttt catttccggg tcgttttccc aaacattgat ccaatcgatc
      961 ggctccgatt ccagaaacat atcaaaagcc gaatacagaa tactttgata ggctcttcga
     1021 tcttcagaat tggtaaggat gacgatgctc cggtttaatg ccggaaaaat ttccattcgt
     1081 ccgcgcatac cgtctactcc gccggaatga ccgacccttt ttttgccctg ataatcctga
     1141 ataaaccaac ccaatccgta tcccgaaaaa tcggtaagca gacggggatg aatcaaatcc
     1201 ttagcgggag ccggcggaac tacaatgtgc atcgaatgca gattttcgac ggattcgggc
     1261 tgtaaaatca cttttccgcg atagacgcca tggttcagga acatttgaag ataatgggct
     1321 aaatcgaggg ctgtcgaatg aacggaagct gccggaccga cattgtcatg atttcgataa
     1381 ggaatcacgc ttaacttgcc attcaactgc gtatggggtg tggcaacatt gtccattccg
     1441 gacagttgca aggctttcag attgggaaaa gagcgttcca tttcaagcgg tgaaaaaata
     1501 tatttacaaa taaaatcatc ccaactcatt ccgcagattt tttcgataat caatccggcg
     1561 atcaaataac acacgttttg ataggcatac gtactgcgaa aaccggtgac gggtctgagg
     1621 taacgaacac gacggactac ttcttcccga gaataatcgg aaccgtacca aagtgtaccg
     1681 gcactttcgc tgttcaagcc gcaatgatgg attaacaagt caaggacggt catttcctgc
     1741 ccggcaaaaa gatcagccat acgaaaatcc ggcaggaatt tggtgacttt atcatcccag
     1801 ccgaacaaat tttgttcccg taatatcgcc aaggaggtgg aaacagttga cttggacatg
     1861 gatgcaatgg cataagaggt atcggcggtg acttcctcgt tttttccgaa tgttcggctc
     1921 ccgaaaccct ctgcgaaact gatttctccg tcttcaatta cagccaccga tatcgccggc
     1981 gtctgaaatt tgatgctcgc attttgaata atctctctga ataattccgg aataattttt
     2041 cccatggttg tcggctttct gtgaaggtaa atgtcagctc ttactatact atgattttcg
     2101 atcggatagc cgctgatctc tcttcgccgg tgatatttcg aaacgattat cgaatcggaa
     2161 tattttccgg ccgaaattcg gctcgaaaaa cggaattcta ctataatgca acatatgtcg
     2221 aaatggattg atatcgccgc acaatttata ggatttccgt tatccgagga ccggcttcga
     2281 ttatttgaaa tttatgaatc cgaactgatc gagtggaata cacgtttcaa tttaaccgcc
     2341 attaaaaacc cggatgaaat tcggacaaaa cattttttgg attcgttatc ctgtgcattg
     2401 gcgatcaacg attttaacgg tcgatcgctg gttgatgtgg ggacgggcgc cggattcccc
     2461 ggaattccgt tgaaaatcgt ttttcccgat ctaaagttga cgttggtgga ttccgtgggg
     2521 aaaaaattgg tattttgtca gcatattatc gatcggctgt ctctgacgga ttgccggatt
     2581 attcatgccc gtgcggaaga tctcggaaag gatccgctcc atcgggaacg gtatgatatc
     2641 gccgttgcca gagcggtaac cgggttatct gccctttcgg aactattgtt acctttggta
     2701 aaaaggaaag ggataatgat cgcacaaaaa ggggagaccg cagcggaaga attgaaagcg
     2761 gcagaaaaag ccatccggat tctgggaggg ggtgaaacac agctcattcc ggtgacaatc
     2821 cccaatgcag tcgagcagcg gtatcttatc cggatcgaaa aacaaagaaa tactccgttg
     2881 ggatacccgc gtcgcccgga tcagattcgc aaaaaaccta t
//
LOCUS       DAOMIV010000253         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_36679_length_2918_cov_4.446036, whole genome shotgun sequence.
ACCESSION   DAOMIV010000253 DAOMIV010000000
VERSION     DAOMIV010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36679_length_2918_cov_4.446036"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PKV59_06460"
     CDS             complement(<1..534)
                     /locus_tag="PKV59_06460"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HQP46432.1"
                     /translation="MTENNHELEKTLWAAADKLRSNMDAAEYKHVVLGLIFLKYISDA
                     FEDLYQKLTENQGEYEGSDPEDPDEYLANNVFFVPEKARWQYLQDRAKQPEIGKYLDD
                     AMDAIERINPPLKGVLPKIYADPELNKQRLGELIDLIGTIGFHQDAHTSQDLLGRVYE
                     YFLGQFAAAEGKKGGQFY"
     gene            910..>2918
                     /locus_tag="PKV59_06465"
     CDS             910..>2918
                     /locus_tag="PKV59_06465"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HQP46433.1"
                     /translation="MDDFSYNSQYLIRNGKPWFPLMGEMHYSRVDRRFWADSLQKMKA
                     GGIDIVSSYVIWIHHEEIEGEFDFSGNKDLRKFIQLCQDHGLYFFLRIGPWVHGEVRH
                     GGFPDWLLAKGFEVRTNDERYFAEVYRFYDRIYSQIKGLQFKEGGPIIGIQIENEYGH
                     CGGLTGSEGEAHMRRLTAMAKEIGFDVPYFTATGWGGAITAGLLPVMGGYPDAPWDSR
                     LTEIEPSGNYIFTPERNDHNIGSDHHIGYGLTFDPAEFPYLTAELGGGLQVTYHRRPV
                     VTAADIGAMTLVKLGSGVNLLGYYMFRGGSNPPGKRSSLQESRAAGDFCDLPQISYDF
                     RAPIREFGQISDTFREIKRFALFLHDFGEELCKMPAKFPSSNPLDPKNNRDLRTAVRD
                     NGKNGYLFVNNYQRRQKMADHPQTKLRVTLPDETIEFPITDIRDGDYFFFPFNLPIGN
                     SILKSAAATPLCKINDDTTVFYSDFPPQYQFDHEPLNHKIITLSKKQAMNAWKIRLPD
                     GDHLILSEDVVLQNRDRLEMIGRGIAELSIYPDFPKPPQNAVRIGGEGDFTVYEVRHP
                     GFDPNRNPAEAKFRIVRQTPEQTDYEITLNYPTNVENCYLSVGYQGDQARLIVDGKLS
                     ADDFYAGTKWEIGLKQFNFPKKIILSVTPLYAGMPVYLEQWPIIEG"
BASE COUNT          753 a          672 c          684 g          809 t
ORIGIN      
        1 ataaaattgg ccgcctttct tcccttcggc ggcggcaaat tgacctaaga aatattcata
       61 aacgcgtccc agcaaatcct gagaggtgtg cgcatcctgg tgaaaaccga tggttccaat
      121 gagatcaatt agttctccca gacgttgttt gtttaactcg ggatccgcat aaatcttagg
      181 cagcacaccc ttaagcggtg ggttaattcg ctcgatcgca tccatcgcat catcgagata
      241 tttaccaatt tccggttgct ttgcccgatc ctgaagatat tgccagcgcg ctttttcagg
      301 gacgaaaaag acattattgg ctaagtactc atccggatct tccggatcgg aaccttcata
      361 ttcgccttgg ttttctgtga gcttttggta cagatcctca aaagcatcgg aaatgtactt
      421 cagaaaaata agtcccagta cgacatgttt atattccgcc gcgtccatat ttgagcgcag
      481 tttatctgcg gctgcccaaa gcgttttttc cagttcatgg ttgttttctg tcatttgctt
      541 ctcctgtcta aaatcgtcac agcggttgcg gtctatcatt gattttcaaa tttcccgggt
      601 ctgggttttg ctttttctgt ttcctaaatc ccttatgaaa tataactctt cgaaatcgct
      661 attgtttgcg gattttaaat tcagaaatga tcttatcctg cgtaagtttg taacctgcgg
      721 gtcaatccca tcaccggtat ttacgaattc attggtaccg aatatgaaaa ccatcgccat
      781 ttcacacagg ccatgagtcc ggatacttta attattatag cattggtcaa gaaaacgatc
      841 ctcgcccggg tttccaagac tgccgccgga ttcattccaa aatcccccag aatcgattaa
      901 gataaattta tggatgattt ttcttataat tcgcaatact tgatccggaa tggaaagcct
      961 tggttcccgc tgatggggga gatgcactat tcccgcgtcg accgccgttt ttgggctgat
     1021 tcgctgcaaa aaatgaaagc cggaggaatc gatattgttt cttcttatgt catctggatt
     1081 catcatgaag aaatcgaggg agaattcgac ttttccggaa ataaggattt acggaaattt
     1141 attcaattgt gccaagatca cggactttat tttttcctga ggatcggacc ctgggttcat
     1201 ggtgaagttc gacatggcgg ctttccggat tggctgctgg cgaagggttt cgaagtgcgc
     1261 acaaatgacg agcgctattt tgcggaagtt tatcggtttt atgaccggat ctattcgcaa
     1321 atcaaaggat tgcaattcaa agagggcgga ccgatcatcg gcatccaaat tgaaaacgag
     1381 tacgggcact gcggcggtct tacaggatcg gagggcgaag cccatatgcg acgtttaacc
     1441 gccatggcaa aggaaatcgg ttttgatgtc ccctatttca ccgccaccgg ttggggcggt
     1501 gccattaccg cagggttatt accggttatg ggaggatacc ccgacgcgcc ttgggacagc
     1561 agattgaccg aaatcgaacc cagcgggaat tacatattta caccggaacg gaacgatcac
     1621 aacatcggat cggatcatca tatcggttat ggattaacat ttgatccggc tgaatttcct
     1681 tatctaaccg ccgaattggg aggcggactg caagttactt atcatcggcg gccggtcgta
     1741 acggcggccg atatcggtgc catgacattg gttaaactcg gatcgggtgt taatttgctc
     1801 ggttattaca tgtttcgcgg cggatcaaac ccgcccggta aacgatcttc cttgcaggaa
     1861 tcgcgagcgg cgggtgactt ttgtgatctg ccgcaaatat catatgactt tcgcgctccg
     1921 attcgggaat tcggacagat ttcggataca tttcgtgaga taaagcggtt tgctctcttt
     1981 cttcatgact ttggtgaaga gctttgtaaa atgccggcta aatttccgag ttcaaatccg
     2041 cttgatccga aaaacaatcg ggatttgcgc acagccgtac gtgataacgg aaaaaacggc
     2101 tatttgtttg tcaataatta ccaacggcga caaaagatgg cggatcatcc gcaaacaaaa
     2161 ttgcgggtga cgttacccga tgagacaatt gagttcccga taacggatat tcgtgacgga
     2221 gattatttct ttttcccgtt caaccttcct atcgggaatt caatccttaa atcagccgcc
     2281 gccacccctc tttgtaaaat caacgatgac acgaccgtct tctattccga ttttccgccg
     2341 caatatcaat tcgatcatga acctttgaat cacaaaatca taaccctcag caaaaagcaa
     2401 gcgatgaatg cctggaaaat tcggcttccg gacggggacc atttgatcct ttcggaagat
     2461 gtggttttac aaaatcggga tcgattggag atgatcggtc ggggaatcgc cgaattgagc
     2521 atatatcccg atttcccgaa gccgccccaa aatgccgtcc ggatcggtgg ggaaggtgat
     2581 tttaccgttt atgaagtgcg gcatccggga tttgatccga atcgaaatcc ggccgaagca
     2641 aagttccgaa tcgtcaggca gacgccggaa cagacagatt atgagatcac cctcaactat
     2701 cccaccaatg ttgaaaactg ttatctttcc gttggttatc agggcgatca ggcaagatta
     2761 atcgtcgacg ggaaactctc agcggatgat ttctatgcgg gaacgaaatg ggaaatcggg
     2821 ctgaaacagt tcaattttcc gaaaaaaatc attttgtccg taacgccttt gtatgccggc
     2881 atgcctgttt atctcgagca atggccgata atcgaagg
//
LOCUS       DAOMIV010000254         2917 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_36700_length_2917_cov_4.042977, whole genome shotgun sequence.
ACCESSION   DAOMIV010000254 DAOMIV010000000
VERSION     DAOMIV010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36700_length_2917_cov_4.042977"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2454)
                     /locus_tag="PKV59_06470"
     CDS             complement(<1..2454)
                     /locus_tag="PKV59_06470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP46434.1"
                     /translation="MNKDVKATEHFFMLSSAEGILCFLYLLFLPGGSGEGSLFAFSRV
                     RLALLSAVLLLACFFGIPLVSKNVLNFWIRLSAKKTFRLFALLLSQGILLSGTALLRS
                     LWLLYRTSQDYSWFVVYRRLFPPVCWLGFIAIQAILYFLYRFSHDFREVFRQKSHQRK
                     TFLILMGIGIVAAVGIAVTRIGLVKDNAFFGKPTVPLLEWHLILAFLLCLLWMILEMK
                     QIGKAAPFVIKAMPFIVWAVAVGIWLAIPNQHGFFSPPGRAPNFEVYPFSDGSFYGHY
                     ARSLAAGMGFKGRDIPPRPLYIVLLAIFHLLIGNQYDSVILLQTLVLGILPALIYLIG
                     KELHSIGAGLAAALLCILRETNSILSAPFAHNVSTTKYFFADLPTALAAAFFVLMLIR
                     WLKAQREDNYRKNVYAVLTGGALGMMTLIRTQSLSLIFVPLAFLIIQTRKQFSRRLSQ
                     FILFTTALCCCLFPWLIRNKQITGKFVFDHPATQTAEMAISYNIGGTDLTNDEGMNEG
                     EYSEKLTRSILTSVKQYPKEIATFITAHFVNNEICNLRLFPLRDRLSRPMELIKPTTP
                     FWEEINNDQLSIYNLIFLALSYALLAFGISASMESHMREGLVPLAVILLFNLSTAVGR
                     YSAGRYLIPVDWIIFLYGGIGIAEWLYRSFRLSGFTPPQTSNEETEREAAQFVQPAKL
                     HPKRKLGLYLGIFLAIGLSLPLSERIIPMRFEPASREAVLSKLEVDPNDYDTADAVVM
                     KAIAIYPRYYAAGEGEPESAKQGYGVSDYGRLIFLTLSPGGFGTIELRTETVPAYFPD
                     NSEIWMVGHSQGATTVADLI"
     gene            complement(2480..>2917)
                     /locus_tag="PKV59_06475"
     CDS             complement(2480..>2917)
                     /locus_tag="PKV59_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46435.1"
                     /translation="SPFVEAGMTKLEIREISKSFQLNTWDQPSTPCLATRIPYGRRID
                     PKDLIKIGKGENYLKSLGIRECRVRLYDSAAAIEVPERDMKIISDHREQIVRIFNEIG
                     FLHTTLDLAGYRRGSLNQGLEKPIAISASGKQASISSDNKIEN"
BASE COUNT          855 a          667 c          677 g          718 t
ORIGIN      
        1 aatcagatcc gctacggtgg tggcaccttg tgaatgaccc accatccaga tctcggaatt
       61 atcggggaaa taagcgggaa cggtttcggt tcgaagctcg atcgtcccga aaccgccggg
      121 agaaagggtc agaaaaatga gccggccata atcggaaact ccgtatccct gttttgcgct
      181 ttccggttca ccctctcccg cggcataata tcgcgggtaa atcgcaatgg ctttcataac
      241 aacagcgtca gcagtatcgt agtcattcgg atcgacttcg agttttgaaa gcacagcttc
      301 ccgggatgcc ggttcgaatc tcatcggaat gattctttcg gaaagaggaa gcgataaacc
      361 gatcgctaaa aaaatcccga gatataaacc gagttttcgc tttggatgaa gtttggcggg
      421 ctgaacgaat tgggcagctt cccgttcggt ttcttcgttg gatgtctgcg gcggagtaaa
      481 accgctcagt cgaaatgatc gatatagcca ttcggctatt ccgattccgc cgtataaaaa
      541 gatgatccag tcaacgggga tcagatatct tccagcagaa taacgtccga cggcggtgct
      601 gagattaaat aacaggatca ccgccaacgg taccaatcct tcgcgcatat gactttccat
      661 ggaagctgag atgccgaatg cgagcagtgc ataggataat gccaaaaaga tcaggttata
      721 gatcgataac tgatcattgt ttatttcttc ccaaaaaggt gtcgtcggct tgattaattc
      781 catcgggcgg cttaatcgat cccgtaacgg gaataatcgc aaattgcaga tttcgttatt
      841 gacaaaatgc gcggttatga aagttgctat ttctttggga tattgtttga cagacgtcag
      901 aattgaacgg gtcagttttt cggaatattc cccttcgttc atcccttcat cgtttgtcag
      961 atccgttcct cctatattgt agctgatcgc catctcggcg gtttgggtag ccgggtgatc
     1021 aaaaacaaat ttaccggtta tttgtttatt gcgaatgagc catgggaata ggcaacaaca
     1081 gagggccgtg gtaaaaagaa tgaattgtga gagacgccgg ctaaattgct ttcgggtctg
     1141 gataatcaaa aaggccagag ggacgaaaat caatgaaaga ctctgagtac gaatcagcgt
     1201 catcattccc aaagctcctc cggttaatac cgcataaaca tttttacgat aattgtcttc
     1261 gcgttgagct ttcagccacc gtatcagcat caatacgaag aacgcagcag ccaaggcggt
     1321 cggtaaatca gcaaaaaagt acttcgtagt ggagacgtta tgcgcaaaag gcgcgctgag
     1381 gatggagttg gtttcccgta aaatacacag caaagccgcc gccaatccgg ctccgatact
     1441 gtgtagttct ttgccgatca gataaatcaa agcgggcaat atccccaata caagggtctg
     1501 taaaagaatc accgagtcat actgatttcc gatcagcaga tgaaaaatgg ctaacaatac
     1561 aatatataaa ggccggggag gaatatctcg gcctttaaaa cccatccccg cagccaaact
     1621 gcgagcataa tgtccgtaaa aggagccgtc cgaaaaagga taaacttcga aattcggcgc
     1681 tctgccgggc ggagagaaaa aaccgtgttg gtttggaatt gccagccaaa tcccgactgc
     1741 aaccgcccaa acaatgaacg gcatggcctt gatcacaaag ggagcggctt tgcctatttg
     1801 tttcatttca agaatcatcc acagaagaca aagcaagaat gccaaaatca gatgccattc
     1861 aagcaaaggg acggtcggct tcccgaaaaa agcattatcc ttgactaagc cgatccgggt
     1921 aacggcgatt ccgaccgcag cgacgatgcc gattcccatc agaatcagga aggtttttct
     1981 ttgatgactt ttttgtcgga aaacttcccg aaaatcatgt gaaaatcgat aaagaaaata
     2041 taaaatcgct tggattgcaa tgaatcccag ccaacaaacc ggagggaaca agcgcctgta
     2101 aaccacgaac caagaataat cttgacttgt tcggtacagc aaccacagag atcgcagtaa
     2161 agccgttccc gacagaagaa ttccctggct taaaagtaat gcgaacaaac gaaaagtttt
     2221 cttggcgctt aaacgaatcc aaaaattcag tacatttttt gaaaccagag ggattccgaa
     2281 aaaacaggcc agcaacagaa ccgccgagag caaagcgagt cgaacgcgac tgaacgcgaa
     2341 aaggctgccc tctccgcttc ctccgggaag aaacaataaa tacaggaagc acaggatccc
     2401 ttccgcggag gacagcatga aaaaatgttc ggttgctttt acatccttgt tcattgttga
     2461 aatttatccg aatgaatgat caattctcga ttttgttatc tgaagaaatg gatgcttgtt
     2521 ttcccgacgc agaaatagcg atcggttttt ccagcccttg atttaaactt ccgcgccgat
     2581 atcccgctaa gtccaatgtg gtatgtaaaa atccgatttc gttgaagatt ctgacaatct
     2641 gttcccgatg atccgaaatg attttcatat ctctttccgg tacttcaatt gcggcagcgg
     2701 aatcgtaaag ccgtacgcgg cattctcgaa tcccgagaga ttttaaataa ttttcgcctt
     2761 ttccgatttt tatgagatct ttcggatcga ttcgtcgacc gtatggaata cgagttgcca
     2821 ggcagggagt ggaaggttga tcccatgtgt tcagttgaaa agattttgat atctcccgaa
     2881 tctccaattt agtcatcccg gcttcgacaa acggact
//
LOCUS       DAOMIV010000255         2911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_36834_length_2911_cov_3.126751, whole genome shotgun sequence.
ACCESSION   DAOMIV010000255 DAOMIV010000000
VERSION     DAOMIV010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36834_length_2911_cov_3.126751"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKV59_06480"
     CDS             <1..887
                     /locus_tag="PKV59_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQP46436.1"
                     /translation="GFYQRGLYYRKDWFKEAGLDVPKTWQDIYDAGLKLTDPSKNRYG
                     WSFRGGTRGYQYADTVYWAYTGVDKLADPNAGYFLKDGDGKTIFTLPEVKEALHFYKS
                     IYKDISPKDSIAWGFSEMVQGFVGGTTAMLIQDPEVIATCSKDMSEDQWDLAPFPIGP
                     SGEAVFPNGFGGWGITSFSKHPDVAADFLLFLSNSENNTYFAKNYSTIPIHINAADMD
                     PYFSEGKFAMYMEMAKQPDVYRYATEPQMYEAFSQFNSEVDQWYQNYLTDQITDDELL
                     AYLDNYWTEAYKNEGKKW"
     gene            998..1903
                     /locus_tag="PKV59_06485"
     CDS             998..1903
                     /locus_tag="PKV59_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP46437.1"
                     /translation="MKTKQKLAGSKNRFIFFMLLPALILPLIFTYYPMFKGSLMAFQS
                     YNLMNVTNIKWIGLGNFQKLFANTPGNIFYTILKNTAIWVIVSLFFQFTIGFALALLL
                     RKKFRGSSVYQGLIFFPWAVSGFLIGIMWRWMFNGTSGVINDILLRIGIIHSPIAWLA
                     SKDTALLSVIIANIWYGIPFFTIMITAALRGVPQELYEAAEVDGANAVGKFFNITIPS
                     IKSVILLTILMRVIWIFNFPDLIYSMTQGGPGGSSHIITSFMMQKVQSLDYGLASALG
                     LLVILFLTVFTIIYFSVTKFGKLED"
     gene            1907..2752
                     /locus_tag="PKV59_06490"
     CDS             1907..2752
                     /locus_tag="PKV59_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP46438.1"
                     /translation="MKSRKIKRRILSTIKFIVLGVYLIIVIFPFFWMLMTSLKSSQGE
                     IYAFPVKYFPETPSLVNYKEMLWKGNFGRYMMNSFIVSGVAAVCAVFIGILAGYVITR
                     FKFKGRGTVLLFFLVTQMVPAFIMLAPLYQMLSKFGMINKLSTLMLLYTNMMIPFSVV
                     TLRGFFASIPSEIEEAAQIDGCGRLRALFSVIVPIMLPGIAATFIFAFINSWNELFMA
                     VMFIDVDAYKTIPVGLNALILKYDIKWGEMAAGTIMALIPTIFLFSFAQKYMVEGLSA
                     GAVKG"
BASE COUNT          755 a          579 c          675 g          902 t
ORIGIN      
        1 atggtttcta ccagagaggc ttgtattatc gaaaagattg gtttaaggaa gccgggctgg
       61 atgtacccaa aacctggcag gatatttatg atgccggttt aaagctgacg gatccctcaa
      121 agaaccggta cggatggtca ttccgcggcg gaacacgcgg ctatcaatat gcggatacag
      181 tatactgggc ttataccgga gtcgataaat tggcggatcc gaacgccggt tacttcctga
      241 aggacgggga cggaaagaca atcttcacct tgccggaagt gaaagaggcg cttcattttt
      301 ataagagtat ctataaagat atttcgccga aggactccat cgcttggggt ttttccgaaa
      361 tggtacaggg atttgtcggt ggcacaaccg ccatgttgat tcaggatccc gaagtgatcg
      421 ctacctgctc caaggatatg tctgaagatc aatgggattt ggctccgttt ccgatcggtc
      481 cgtcgggtga agcggtattc cccaacggct tcggaggatg gggaatcact tccttctcca
      541 aacatcccga tgtcgcagcc gatttcctgc tgttcctttc caattcggaa aataatacct
      601 attttgcgaa gaattactcc accattccga ttcatatcaa cgcggcggat atggatccgt
      661 atttctccga agggaagttt gccatgtata tggaaatggc aaaacaaccg gatgtttatc
      721 gttatgccac agaaccgcaa atgtatgagg ccttctcaca attcaacagc gaagtggatc
      781 agtggtacca aaactatctg accgaccaga taaccgatga cgaattattg gcttatctgg
      841 ataattactg gaccgaagcc tataaaaacg aaggcaagaa gtggtaagaa ttaattatcc
      901 ggaataatta tcggttcggt taaaatgaaa gaggctgcct tccacctttt gtatcaggaa
      961 tgccgaggca gccttctttt atgccgggag gttcaatatg aaaacaaaac aaaaattagc
     1021 cggtagcaag aatcgattta tttttttcat gctcttgccg gctttgatct taccgctgat
     1081 ttttacctat tatccgatgt tcaaaggatc tttgatggca tttcaaagct acaatttgat
     1141 gaatgtcacc aatattaaat ggatcggatt gggtaatttt caaaagttat tcgcaaatac
     1201 accggggaac attttttata ccatcttaaa aaataccgcg atatgggtga ttgtatcttt
     1261 gtttttccaa tttaccatcg gttttgcctt ggctttattg ttgcgtaaaa aatttcgcgg
     1321 ttcatctgtc tatcagggat tgattttctt tccctgggcg gtttcgggat tcttgatcgg
     1381 aatcatgtgg cgctggatgt tcaacggcac ctcgggcgtc attaatgata tcttgttgcg
     1441 gatcggaatt atccattctc caatcgcctg gttggcgagt aaagatactg cgctcctttc
     1501 agttattatc gccaatatct ggtatgggat tccgtttttt acgattatga ttactgctgc
     1561 gttacgcggt gtaccgcagg aattatatga agccgccgaa gttgacggcg cgaatgccgt
     1621 cggaaaattc ttcaacatta ccattccgag tatcaaatcg gtgatcctgc tgaccatatt
     1681 aatgagagtc atctggatct ttaattttcc ggatttgatt tattcaatga cgcagggagg
     1741 accgggcgga tcttcgcata tcatcacctc ttttatgatg caaaaagtgc aaagtttgga
     1801 ttacgggtta gcttccgcat tgggattatt ggtgatttta ttcctgaccg tctttacgat
     1861 tatctatttt tccgttacca agttcggcaa actggaggat taaacgatga aatctcgaaa
     1921 aataaaacgc cggattcttt cgacaatcaa attcattgtt ctgggagtct atctgattat
     1981 tgtcatcttt cctttttttt ggatgctgat gacttccttg aaatcttctc agggagaaat
     2041 ctacgctttt ccggtaaagt attttccgga aaccccttct ctggtgaatt ataaggaaat
     2101 gctttggaag gggaacttcg ggcgatatat gatgaattct ttcattgttt ccggcgttgc
     2161 ggctgtctgt gcggttttta tcggaatttt ggcgggctat gtgattacgc gatttaaatt
     2221 caaaggtcgg ggaaccgttc tgttgttttt cttggtaacg caaatggtcc cggcttttat
     2281 catgttggca cctttgtatc aaatgctctc gaaatttggg atgattaata aattatcgac
     2341 attaatgctg ctttatacca acatgatgat cccattttcc gtggtgacgt tgcgcggctt
     2401 ctttgcctcg attccatcgg aaattgagga ggctgcccaa attgacggtt gcggcagact
     2461 acgggcgtta ttttccgtca tcgtgccgat tatgcttccc gggatcgcag ctactttcat
     2521 ttttgctttt atcaactcat ggaatgaatt atttatggcg gtgatgttca ttgacgtcga
     2581 tgcttataag acgattccgg tgggactgaa tgctttgata ttgaaatatg atattaaatg
     2641 gggtgagatg gcagccggga caattatggc gctgatcccc acaattttcc tgttttcttt
     2701 cgctcagaaa tatatggtcg aaggactgtc agccggcgcg gtaaaaggtt gatcggagaa
     2761 tttataagga gactcggaga agaattttat gctcaatgaa atgcaaaatg ccaagatgat
     2821 ctataaattg aaacggtgtc tgacggtttt cgaaccatat attttcagaa cgatcgatcg
     2881 aattccttat cgaatttatc aaacaagaga a
//
LOCUS       DAOMIV010000256         2901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_37009_length_2901_cov_3.545327, whole genome shotgun sequence.
ACCESSION   DAOMIV010000256 DAOMIV010000000
VERSION     DAOMIV010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37009_length_2901_cov_3.545327"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PKV59_06495"
     CDS             <1..220
                     /locus_tag="PKV59_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HQP46439.1"
                     /translation="VPVLGTVCMDQCMLDLNEVPDAVTADEAIILGSQGDDRISAEEL
                     GARWHTINYDVVCGIAERVTRLYTPELS"
     gene            244..1938
                     /gene="recJ"
                     /locus_tag="PKV59_06500"
     CDS             244..1938
                     /gene="recJ"
                     /locus_tag="PKV59_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQP46440.1"
                     /translation="MEPLFEWKLPSKIPLDVLEQLASFPRVQQRLLYNRGIRDSVGAN
                     SYLRRQGSLYDPFLLDQMDLAVKTILQAIDSRMKIAVYGDYDVDGVTASALLIQVLTA
                     LGAEVRGYIPNRFEEGYGVNKEALHTLHEEGIALTITVDCGIRSPEETFFAHSLGMEM
                     IITDHHEPGSTIPEAVAVICPKKPSDSYPDKNLAGVGLAYKLAEALLTARPVAGIAAE
                     DWEDFVAVGTVADMVSLTGENRSLVKSGLQKLRVSSRPCIRALAEVSGIRMADIKAST
                     IGFSFGPRLNAAGRLETAKLAFDLLMSRTLNEARRFAIELNDQNRQRQELTKLTQRQV
                     LEEINPARVPILIFSAKEEFNRGVVGLAASRLAEHFYRPAIVGSINGDEIHASCRSIL
                     EFHITDALDQCADLMVKHGGHAMAAGLTIKKDNWSMFVDKMTTIATQRLQDQPLKPVK
                     QVDMEIPLDYLKENVLEQIAEMEPFGMDNPEPLFLIRNLKVASSRIIGNDLSHLRLKL
                     ISEDQITVDAVAFRQGWRRNDLPERIDVLGTFEKNTWKDQQTLQLNLVDFRSAVSN"
     gene            complement(1959..2642)
                     /locus_tag="PKV59_06505"
     CDS             complement(1959..2642)
                     /locus_tag="PKV59_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQP46441.1"
                     /translation="MDLTVIMPVFNEEKTLREIISKVKETGLANEILVVDDGSTDGTK
                     EILKEYQDDPVVRVITSEKNEGKGAAVRKGILNARCEYAIIQDADLEYNPKFYPVLMQ
                     PIKDGIADVVYGSRFLGSARRPILFWNMVANKILTFTTNILYNNILSDMETGYKLFKV
                     SMVREIPLHAHGFDFEPEFTAKVLKRKFRIYEVPIDFTPRYYTEGKKIKAKDGFVALW
                     TLIKYRFVD"
     gene            complement(2655..2831)
                     /locus_tag="PKV59_06510"
     CDS             complement(2655..2831)
                     /locus_tag="PKV59_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46442.1"
                     /translation="MSAPRNLGVLRKDAIPYMVTFIAKTPFHHIVNILRGGAIPKKSY
                     KRCRQIIGYTNQKN"
BASE COUNT          755 a          687 c          703 g          756 t
ORIGIN      
        1 ggttccggtt ttgggaacgg tttgcatgga ccaatgtatg ctcgatctca atgaagtgcc
       61 cgacgccgtg accgccgatg aagcgatcat ccttggttcg cagggtgatg accggatcag
      121 tgcggaagag ttgggagctc gctggcatac gattaattat gatgtggttt gcggtatcgc
      181 cgagcgggtt acccgtcttt acacgccgga actctcttaa tccctttcgg gaggaaaact
      241 tttttggaac cgctttttga atggaaatta ccctcaaaaa tccccttgga tgtgcttgag
      301 cagcttgcat ctttcccgcg cgtccaacaa cgtttgctct ataatcgcgg tatcagagat
      361 tccgtcggag caaacagcta cttacgcagg cagggttcac tttatgatcc gttcctgctg
      421 gatcaaatgg atcttgccgt aaaaaccatc cttcaggcaa tcgatagccg tatgaaaata
      481 gcggtttacg gcgactatga cgtggatggt gtgacggcat ccgccctgct gatccaggtt
      541 ttaaccgctc tgggagcgga agttcgcggt tacatcccca atcgattcga ggaaggatac
      601 ggcgttaata aagaggcgct gcatacctta catgaagaag gcattgcact gacaattact
      661 gtcgactgcg gcattcgctc gcccgaagaa acgttctttg cccattcgct cggaatggaa
      721 atgatcatca cggatcatca cgaaccgggc agcactattc ccgaagcggt ggcggttatt
      781 tgccccaaaa aaccttccga ttcttatccg gataaaaacc tggcaggcgt cgggttggca
      841 tataaattag cggaagcatt attgacagcc cgcccggtcg cagggatcgc ggccgaagat
      901 tgggaggatt ttgtggctgt cgggacggtt gcggatatgg tttcactgac cggcgaaaat
      961 cgcagtctcg tcaaaagcgg actgcaaaag ttgcgggttt cgtcgcgtcc ctgcatccga
     1021 gctttagcgg aagtctcggg tatccgaatg gcggatatca aagcttcgac gattggattc
     1081 tctttcggac cacgcttgaa tgccgccggt cggttggaaa cggctaaact ggcttttgat
     1141 ctattaatga gtcggacgct caacgaagcg cgcagattcg ctatcgaatt gaatgatcaa
     1201 aaccgccaac ggcaggaatt aaccaaactg actcaacgac aagtgttgga agaaattaat
     1261 cccgcgcggg tacccattct gattttctcc gcaaaagaag aattcaaccg cggggttgtc
     1321 ggattggcag cctcaaggct ggcggaacat ttttatcgtc cggccattgt cggatcgatc
     1381 aacggcgatg aaattcatgc gtcttgccgc agcattctgg aattccatat caccgatgcg
     1441 ctcgatcaat gcgcggactt aatggtaaag cacggcggtc atgccatggc tgccggactg
     1501 acgattaaaa aagacaactg gtcaatgttt gtcgataaga tgacaacgat cgcgacacaa
     1561 cgtctgcaag accaaccgtt gaagcccgtc aaacaagtcg atatggaaat tccgttggat
     1621 tatctgaaag aaaacgtatt ggaacaaatc gcagaaatgg aaccgtttgg gatggacaat
     1681 ccggaaccgc tctttttgat ccgaaatctg aaagtcgcat cttcccggat aatcgggaat
     1741 gacttaagtc atctgcggtt gaagcttata tccgaagacc aaattaccgt cgacgccgtc
     1801 gcatttcggc agggttggcg ccgaaatgat ttgccggaac ggatcgatgt cttagggact
     1861 tttgaaaaaa atacctggaa ggatcagcaa acccttcaac tcaatctggt tgatttccgt
     1921 tccgcggttt cgaattgaaa tcgtgaaatc cgctccgatt aatccacgaa ccgatattta
     1981 atcaatgtcc ataaagcgac aaaaccatcc ttggctttga tttttttgcc ttcggtgtaa
     2041 tatcgcggtg taaaatcgat cggaacctca taaatccgaa attttcgttt cagaaccttg
     2101 gcagtaaact cgggttcaaa atcaaaacca tgcgcatgca gcggaatttc acgtaccata
     2161 gaaaccttga ataatttata accggtttcc atatccgaaa gaatattgtt atataagata
     2221 tttgtcgtaa aggtcagaat cttattggct accatattcc agaaaagaat cgggcgacgg
     2281 gcggagccta aaaaacgaga tccataaacc acatccgcaa ttccgtcttt gatcggctgc
     2341 atcaataccg gataaaactt ggggttgtat tctaaatcgg catcctgaat gatcgcatat
     2401 tcgcagcggg cgttcaaaat cccttttcgg acggcagcgc ctttcccctc gtttttctca
     2461 cttgtgatca ctcgcaccac cggatcatcc tgatattctt tgagaatttc cttcgttccg
     2521 tccgttgaac cgtcatccac aaccaaaatt tcatttgcca gacccgtttc tttgactttg
     2581 gaaataattt cgcgaagtgt tttctcctcg ttaaataccg gcatgatcac agtcagatcc
     2641 attcaaatcc atccttaatt tttttgatta gtatacccga taatttgccg gcagcgtttg
     2701 taagattttt tggggatggc gcccccgcgg agtatattga ctatgtggtg gaatggcgtc
     2761 ttcgcaataa acgtgaccat gtaggggatg gcgtccttgc gaagcacacc gaggttacga
     2821 ggtgcagaca tcccgtgaac gcgaagtgtt catgtatatt ccatatttac caatgctcat
     2881 tcatataaaa cagtgtaggg g
//
LOCUS       DAOMIV010000257         2893 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_37153_length_2893_cov_4.352361, whole genome shotgun sequence.
ACCESSION   DAOMIV010000257 DAOMIV010000000
VERSION     DAOMIV010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37153_length_2893_cov_4.352361"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="PKV59_06515"
     CDS             complement(<1..1148)
                     /locus_tag="PKV59_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46443.1"
                     /translation="MDKEIIVEMKHITKLFPGVVANDDISISIQKGEIYALLGENGAG
                     KSTLMSILFGLYQPDHGEIYIRGQKVTLESSRTAAELGIGMVHQHFKLIDNYTIAENI
                     ILCREPIHRLLGFLPAVDLKKANKEIRELSQKFHLEVDPEQKIEDINVSVQQRVEILK
                     MLYREADILIFDEPTAILTPQEIEYLLDIMQELRRNGKTIILITHKLEEVKKIADRCA
                     ILRRGKLIKVLDVSRTSTLEMANLMVGKEFSFDINKTPARCGDVVLDVEHLSVRSENK
                     VEVVHDVSFKVHQGEIFAIAGVSGNGQVEIADAVAGLIPVHSGTIRLNGRDITNLDVR
                     ERTEAGISYIPEDRQQVGMVADMTLAENMAIKSYYREPFAKRGILQPEV"
     gene            complement(1287..2009)
                     /gene="deoD"
                     /locus_tag="PKV59_06520"
     CDS             complement(1287..2009)
                     /gene="deoD"
                     /locus_tag="PKV59_06520"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQP46444.1"
                     /translation="MTIVPSECNEALPGEIAKVVLMPGDPLRAKFVAENYFESPHLFN
                     QLRNMYGYTGIYKGKQISVMGSGMGIPSLGLYASDLYEYYGVEYIIRIGSAGGLADDI
                     RLRDIVIAMSASTNSAYASQYDFPGILAPTADFDLLRKAVKNGEAIGAHLHVGPVFSS
                     DVFKNARKDANERYRDMGMLAVEMETAGLYMTAAYYHKKALSILTISDHVFRQEGLSA
                     IERQESFREMMEIALETAYQSI"
     gene            complement(2026..>2893)
                     /locus_tag="PKV59_06525"
     CDS             complement(2026..>2893)
                     /locus_tag="PKV59_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46445.1"
                     /translation="DIITDYDAIVLPGFQFAAVGTVAQENPNTKLILVDTPPTDAEGN
                     TVELPNVYSMTFKEEEGGFFAGIAAAMETKTNKVAVVNGLAYPSNVNYQWGFESGVNY
                     ANAKFGTKAEIVELPSYAGTDVTGKNVGGNYIGGFADEVTGKVVGKALIDKGVDIIFV
                     AAGASGNGVFTAIKEANGVYAIGCDVDQFDDGVKGDGSNMILTSTLKVMHTNVTKQLQ
                     AIADGTFVGKNEVLGASTDSTGYVSAEGRQQLSADTLAKLAEAYQLVKEGKIVPAANF
                     NGYTPEDFPGLK"
BASE COUNT          724 a          712 c          637 g          820 t
ORIGIN      
        1 acttccggct gcagaattcc tctttttgca aaaggctccc gataatagga tttgatcgcc
       61 atattttcag ccagtgtcat atccgccacc atcccgactt gttgacggtc ttcgggaata
      121 taggatattc cggcctccgt tcgctcgcgg acatccaggt ttgtaatgtc ccggccattg
      181 agacggatgg tacccgagtg aaccggaatc aaaccggcaa cggcatccgc gatttccacc
      241 tgtccattgc cggacacacc ggcaatggcg aatatttcgc cttgatgcac tttgaaagaa
      301 acatcatgca cgacttccac tttgttttcg ctacgaacgg aaagatgttc gacatcgagg
      361 accacgtcgc cgcagcgagc gggcgttttg ttgatatcaa atgaaaattc tttccctacc
      421 attaaattag ccatttcaag cgttgaagtc cggctgacat ccaatacttt gatcagcttt
      481 ccgcgccgta aaatggcaca gcgatcggca atttttttga cttcttctaa cttatgggtg
      541 atcaggataa tcgttttccc gttacgtcgc aattcttgca tgatatccag aagatattcg
      601 atttcctgtg gggttaggat cgcggtgggc tcatcaaaaa tgaggatatc cgcttctcga
      661 tatagcattt tcaagatttc cacccgttgc tggaccgaaa cgttgatgtc ttcgattttt
      721 tgttcgggat cgacttccaa gtgaaatttt tgtgaaagtt cgcgaatttc tttgtttgcc
      781 tttttgaggt cgaccgccgg cagaaatccg agcagacggt ggatcggttc gcggcagaga
      841 atgatatttt cggcgatggt gtaattatcg atcaatttaa aatgttgatg gaccatgccg
      901 atccccagtt cggcagcggt gcgggaggat tccaatgtga ctttttgacc gcgaatatat
      961 atttctccgt gatccggttg atataaaccg aaaaggatac tcatcagagt tgattttccc
     1021 gctccgtttt caccgagcaa cgcatatatt tccccttttt ggatactgat ggaaatatca
     1081 tcgtttgcca caacccccgg gaaaagcttc gttatatgtt tcatttcaac gatgatttct
     1141 ttatccataa aaacttactc catcgttcgt atttctcatc ggtcaacctc aaacaatgta
     1201 aaaaatcgga atttgtgcgg atggaaaaac ttgtcattcc ggatcggatt gccctgtttc
     1261 ggaatgacaa gtggatcata atttaattaa attgattgat aagccgtttc cagagcgatt
     1321 tccatcattt cgcgaaacga ctcctgacgc tcaatcgcag ataacccttc ctgtcgaaaa
     1381 acatgatcgg agatcgtcag gatactgagc gctttcttgt gataataagc cgccgtcata
     1441 tagagcccgg cggtttccat ttccactgcc aacatcccca tatcccgata acgttcattg
     1501 gcgtcttttc gggcattttt gaacacatcg gatgaaaata ccggaccgac gtgaagatga
     1561 gcgccgatgg cttcaccgtt tttcaccgct tttctcaata aatcaaaatc agcggtaggc
     1621 gccagaattc cggggaaatc atattgagac gcataagcgg aatttgtcga agctgacatg
     1681 gcaataacga tatctcgcaa ccggatatcg tccgctaagc cgccggccga accgatacga
     1741 ataatatatt ccactccgta atattcatat aaatccgaag cgtataaccc cagagacggg
     1801 atgcccatgc cgctacccat gaccgatatt tgtttgcctt tgtaaatccc cgtatagcca
     1861 tacatgttgc gaagctgatt aaataagtgc ggggattcga aataattttc cgcgacaaac
     1921 ttcgcccgga gaggatctcc gggcatcaga accactttcg cgatttctcc gggcaacgct
     1981 tcattgcatt cggaaggtac gattgtcata aaacgtttac cttgcttatt tcaagcccgg
     2041 aaaatcttcc ggtgtataac cattgaaatt cgcggcgggg acgatttttc cttctttgac
     2101 caactggtag gcttcagcca gttttgctaa ggtatcggcg gataattgtt gccgcccttc
     2161 cgcgctgaca taaccggtgg aatcggttga ggcgcctaaa acttcatttt taccgacaaa
     2221 agtaccatcg gcgattgcct gaagttgttt tgtgacgttt gtatgcatca ccttgagggt
     2281 tgaggtcaga atcatattgc ttccgtcacc cttaacaccg tcatcgaatt gatcgacatc
     2341 gcatccgatt gcatagacac cattggcttc cttgatagcg gtgaaaacac cgtttccgga
     2401 agcgcccgct gcgacaaata taatatccac acctttatct atcagcgctt tgccgactac
     2461 tttaccggtg acttcatccg cgaaaccgcc gatataattg ccgccgacat tctttccggt
     2521 cacatcggtt ccggcatagg agggcaattc cacgatttca gcttttgtac cgaatttggc
     2581 attcgcatag ttaacgcctg actcaaaacc ccattgataa ttcacattgg aaggatatgc
     2641 caagccgttg acgactgcga ctttattggt ttttgtttcc atcgccgcag caattccggc
     2701 gaagaaacca ccctcttctt ctttaaacgt catggaatag acatttggta attcgacggt
     2761 atttccttcg gcatcggtcg gcggagtatc gaccaaaatt aacttggtgt tcggattttc
     2821 ctgggcgacg gttccgaccg cggcaaattg gaatcccggc agtacaatcg catcgtaatc
     2881 ggtgataatg tcg
//
LOCUS       DAOMIV010000258         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_37307_length_2885_cov_4.243463, whole genome shotgun sequence.
ACCESSION   DAOMIV010000258 DAOMIV010000000
VERSION     DAOMIV010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37307_length_2885_cov_4.243463"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PKV59_06530"
     CDS             <1..879
                     /locus_tag="PKV59_06530"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HQP46446.1"
                     /translation="FLGIDIQYYAVISFEGFVNFINSIDKLAINVKDQITVDPIGPGN
                     TVTLYPGVQDLDGATALAYARQRYTEGGDFERAQRQQDVIYALYEQMVWQLPQLLLHA
                     DKLYNTISQAIRTNLTFDDIIKLAWTVVDIKPEQIVRSVIAPPYQVQYGKTYDGTQDI
                     LIPIPDKIREARDVIFSTSLAASPATVADNQATLIAAEGAHISLLNGSTNPDIMDKTV
                     AYLQQFGITIDSIGQAANSYTNSLVINKGKPFTAKFLADLMAIPTAAITMDYQPDAAS
                     DLVLTITDAWANSNPM"
     gene            944..2512
                     /gene="gpmI"
                     /locus_tag="PKV59_06535"
     CDS             944..2512
                     /gene="gpmI"
                     /locus_tag="PKV59_06535"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HQP46447.1"
                     /translation="MEKPVVLVIMDGVGIGKGDIGDAVKKANTPTLDWLRSHCPYTQI
                     KAHGTAVGLPSDEDMGNSEVGHNALGCGQIYSQGAKLVNESIENGSIFHSETWNKLIG
                     NCVENHSTLHFIGLLSDGNVHSNINHLKAMIREAKNQKVSRVRIHILLDGRDVPSTSA
                     LQYVNDLESFLKTLNDNSFDARIASGGGRMKVTMDRYQADWNMVKIGWDTHVLGIGRQ
                     FANAAEAIETYRKELEVIDQDLPAFVIAENGKPIGTIEDNDSVIFFNFRGDRAIEISM
                     AFDYPDFDKFDRVRYPKVFYAGMLQYDGDLKLPKNFLVNPPNIQHTLTELLVSRHLSQ
                     YAISETQKFGHVTYFWNGNRSGKLSEELETFEEIPSDMVSFDERPWMKSAEITDKMLS
                     AISSGKYDFIRCNYPNGDMVGHTGNFDATVIAVEAVDLELARIVKLMESHDFTLVVTA
                     DHGNADDMIEKTKSGELQPKTSHSLNPVPFMIYDKTREWKIKEGSYGLANVTGTLVKL
                     MGLEPPEIWEKPMI"
BASE COUNT          835 a          622 c          655 g          773 t
ORIGIN      
        1 ttcctcggaa tcgatattca gtattacgct gtgatttctt ttgagggttt cgtcaatttc
       61 attaattcca tcgataaatt ggcaatcaac gtcaaggatc agatcacggt tgacccgatc
      121 ggtccgggaa acaccgtcac actttacccc ggtgtgcaag acttagacgg tgcgactgca
      181 ttggcatacg cccgtcaacg atataccgaa ggcggcgatt ttgagcgagc ccaacgccag
      241 caggacgtta tttatgcttt gtacgaacaa atggtctggc agctgcctca acttctactc
      301 cacgccgata agctttacaa caccatatcc caagcgatca gaaccaatct gactttcgac
      361 gatattatca aactggcatg gacggtggtg gatatcaaac cggaacaaat cgtccgaagc
      421 gtcatcgctc ctccgtatca ggtacagtac ggtaaaacct atgacggtac tcaggatatt
      481 ctcattccaa ttccggataa aatccgagaa gcaagggatg tcattttcag caccagtctt
      541 gccgcatctc cggctaccgt tgccgataat caggcgactt tgatcgctgc ggaaggcgct
      601 catatcagcc tattgaacgg ctcgacaaat cccgatatta tggacaagac tgttgcctat
      661 ttgcaacaat tcgggatcac aatcgattca atcggacaag cggcgaattc ttatacaaat
      721 tcgctggtaa tcaataaagg gaaaccgttt acggcgaaat tcctggcaga tctgatggcc
      781 atcccaactg ctgcgatcac aatggattat caaccggacg cagcctcgga tttggttctg
      841 accattacgg acgcttgggc aaacagcaac cccatgtaac aataacgcaa ctccggattt
      901 catgtaacat ccggagttgt aatttatcaa caatcggagc aatatggaaa aaccagttgt
      961 tttagtcatt atggatggcg tcggtatcgg aaagggtgat ataggtgacg ccgtaaaaaa
     1021 agcgaatacc cccacattgg attggctgcg cagccattgt ccgtatactc aaatcaaagc
     1081 tcacggcaca gcggtggggc ttccgtcgga tgaagatatg gggaacagcg aagtgggtca
     1141 taacgcactc ggctgcggtc aaatttactc acagggagcc aaactcgtca atgagagcat
     1201 tgagaacgga tccatttttc attccgaaac ctggaataaa ctgatcggaa attgtgtaga
     1261 aaatcattcg acactccatt ttatcggtct tctttccgac ggaaatgttc attccaatat
     1321 caatcatctg aaagcgatga ttcgggaagc aaaaaatcaa aaagtctctc gcgtacggat
     1381 tcatatttta ttggacggcc gagatgtccc ctcgacttcg gcactgcaat atgtcaatga
     1441 tttggaatct tttttaaaaa cgctcaacga caattcgttt gacgccagga tcgcatccgg
     1501 cggcggacga atgaaagtaa cgatggaccg ctaccaggcg gactggaata tggtcaagat
     1561 cggttgggat acgcacgtac tgggcatcgg caggcagttt gctaacgccg cggaagcgat
     1621 tgagacctat cgaaaagaat tagaggttat cgatcaggat ctgccggctt ttgttattgc
     1681 cgagaatggc aagccgatcg gaaccattga agataacgac agtgttattt tcttcaattt
     1741 ccgtggagat cgtgcgattg aaatcagtat ggcttttgat tacccggact ttgataaatt
     1801 tgaccgtgtc cgttatccga aagtttttta tgcgggtatg ctgcaatatg acggcgacct
     1861 gaagctccca aagaatttct tggtgaaccc gccgaacatt caacatacgc ttacggaatt
     1921 gctcgtcagc cggcacctca gccaatatgc catttccgaa actcagaaat ttgggcatgt
     1981 cacctatttc tggaacggga accggagcgg taaattaagt gaagaattag aaactttcga
     2041 agaaattcct tccgatatgg tgtcttttga cgaacgacct tggatgaaat cggctgaaat
     2101 aaccgataaa atgctgagcg cgatctcctc cggaaaatat gatttcattc gctgcaatta
     2161 tccgaacgga gatatggtcg gacacaccgg gaactttgat gcaacggtta tcgccgtcga
     2221 agcggttgat ttggaacttg cgcggatcgt taaactgatg gaatcacatg attttacctt
     2281 ggttgttacg gctgatcacg ggaatgcgga tgatatgatc gagaagacaa agtccggaga
     2341 attacagccg aaaacttccc attcgctcaa tccggtacct tttatgattt acgataaaac
     2401 ccgtgagtgg aaaatcaaag agggcagcta cggtttggca aacgttaccg gaacactggt
     2461 gaaattaatg ggattggaac cgccggaaat ttgggaaaaa ccgatgatct gatcggtgac
     2521 ggtacgcgcg agacaaaaaa agaatatggt tcaaaaaagg atcggatttg aaaatccgat
     2581 ccttttttat gaaagaccga ttttcttcgt tatcgggtaa ttttttttga ttcctctttt
     2641 atgaattttt gcatttttcc aaataatctg aatatttgtt attaacacta tgttataaac
     2701 atcaacttat tccgggttag aattacttat taacgtttta atttaacgct tatttcagtt
     2761 cgatttggac aaatatcaca tttaattaag attaattact tgagtggcca tgttattatt
     2821 gcatataatg ttaataataa agcagaaaat attcgtccga ctttgtcgat aaacgagatt
     2881 tgaat
//
LOCUS       DAOMIV010000259         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_37451_length_2878_cov_3.030110, whole genome shotgun sequence.
ACCESSION   DAOMIV010000259 DAOMIV010000000
VERSION     DAOMIV010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37451_length_2878_cov_3.030110"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PKV59_06540"
     CDS             complement(<1..415)
                     /locus_tag="PKV59_06540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQP46448.1"
                     /translation="MTTASDSRPPAFHVMAKPRGAICNLGCEYCYYLPKKALYPDSTF
                     RMSNQVLKSYVRQYIAAQQVPQIHFAWQGGEPTLMGLDFFRRVVGYQKEAARPGMRIE
                     NAIQTNGTLLNDDWCRFFAENHFLVGISLDGPREIH"
     gene            complement(484..1317)
                     /locus_tag="PKV59_06545"
     CDS             complement(484..1317)
                     /locus_tag="PKV59_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP46449.1"
                     /translation="MKKKTFGALFRHGFLLAVVIYTLIPIFFTLGTSLKFFRDIISGS
                     LLFKPTLINYERLLNPSQSNFLRLTTNSLLIAAGTMAVVLVIASLAAYSLSRFNWHPL
                     WNGLIQGIILFIHMMPPVTFLSPLYLISRALNIYDTPVAVIMGHIILTLPTAIWILLD
                     FFAEIPKEIEEASIVDGASHFQSLTQVMLPIIQPGLASAGALTFIFSWRDFLFALSLT
                     TTPRGMTIPVGISSFVQEYSIRYGEMAAGSFFAMIPALLMVAFAQRYIIKGMTMGALK
                     E"
     gene            complement(1338..2267)
                     /locus_tag="PKV59_06550"
     CDS             complement(1338..2267)
                     /locus_tag="PKV59_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP46450.1"
                     /translation="MDHSTYFSLSSKPPGWKTRFSNFIDRTFKIWTILPLILVLIFLV
                     AYPTVQLLRMSVSEVKIVQGKTVWTFVGLKHLYTAMEDQIVPVALRNTIVFVIAVVIL
                     ETILALVIALLVSRSHHLVGLYRTVFLIPLLIPPVAIGTMWRLMYDYNYGFINQVLGL
                     IGIAGPTWTADKKLALPSIILVDLWHWTSFMFLIILAGLESLPQEINEAARVDGASEA
                     QVFRFITLPLLKPTLVTAVMLRTIFAFKVFDEIYLLTGGGPGTATEMISLYIYKVFSN
                     QFRMGYASFLALALAIIISVFVIFYRRIGRRLA"
     gene            complement(2428..>2878)
                     /locus_tag="PKV59_06555"
     CDS             complement(2428..>2878)
                     /locus_tag="PKV59_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQP46451.1"
                     /translation="QGKVAAVPVPGATAGSNATMSGIWVMSIPHNLPKERKEAALAFL
                     KWAMTKDAQLYYAQAGAIPVRQDVYEEMSQDPKVGWWMKAMADSTPFIHAQPRLVETP
                     QIIEVIDRRLKQALISETTPEETLSEAAKEIFKILKDAGYKVKPLAE"
BASE COUNT          869 a          686 c          615 g          708 t
ORIGIN      
        1 catgtatttc ccgcggacca tccaagctga ttcccaccaa aaaatgattt tccgcgaaga
       61 atcggcacca atcatcgttt aataaagtcc cgttcgtttg aattgcgttc tcgattctca
      121 ttccgggacg tgccgcttct ttctgataac cgacgaccct ccgaaaaaaa tccaacccca
      181 tcagcgtggg ttccccaccc tgccaagcaa aatgaatttg cggaacctgc tgcgccgcaa
      241 tatactgccg cacataactt tttaaaactt gattcgacat tctgaaagtg ctgtcgggat
      301 ataaagcttt tttgggcaga taatagcaat attcacatcc caaattacag attgctcccc
      361 gcggttttgc cataacatgg aaagcgggag gtcttgaatc agatgcggtt gtcactcgaa
      421 tctcaccgat gataatttat tttcacataa ggaagccatt ctcatccccg acatcttcca
      481 attttactct tttaaagcac ccattgtcat tcctttaata atatatcgct gggcgaacgc
      541 aaccatcaat aaagccggaa tcatggcaaa gaaggatccg gctgccattt cgccgtaacg
      601 aatactgtat tcctgcacaa agctggaaat tccgaccgga attgtcatac cgcgtggagt
      661 tgtcgtcaga gaaagtgcga ataaaaagtc acgccaagag aaaataaagg ttaaagcgcc
      721 ggcggaagcc aagccgggtt gaataatcgg aagcatcacc tgagttaacg actgaaaatg
      781 gcttgctccg tcaacgatag aggcttcttc gatctctttg ggaatctccg caaaaaagtc
      841 cagcaatatc catatagccg tcggtaaagt taagataata tgtcccataa taaccgctac
      901 cggcgtatca tagatattta acgctcggga aattaaatag agtggactta agaaggtaac
      961 gggcggcatc atatgaataa aaaggataat tccctgaatc aatccgttcc ataacggatg
     1021 ccaattaaac cggctgaggc tgtaagcagc caaactggcg atcaccaaca caacagccat
     1081 cgttccggcc gcgatcaata agctgttcgt tgtcaaccgc aaaaaattgc tttgcgaggg
     1141 gttcagcaat cgctcataat tgattaaggt cggtttaaat aataatgaac cggaaataat
     1201 atcccgaaag aactttaacg atgttcccaa ggtaaaaaag atgggaatta aagtgtaaat
     1261 taccaccgct aaaagaaatc cgtgtctgaa aagcgctccg aaagttttct ttttcatagg
     1321 cgtgttctcc ttccgaatca agcgaggcgg cggccgatac gccgataaaa gatgacaaaa
     1381 acagagataa tgattgccag cgccaaggca agaaaagagg catatcccat cctgaattgg
     1441 tttgaaaata ccttataaat atagaggctg atcatttcgg tcgcggtacc cggtccgccg
     1501 ccggtcagca ggtatatttc atcaaatact ttgaaagcga aaatggttcg cagcatcaca
     1561 gcggttacaa gggtcggctt caggagaggc agtgtaataa agcgaaacac ctgtgcttcg
     1621 gatgcgccgt ccacccgcgc agcttcatta atttcctggg gtaaagattc caaaccggcc
     1681 aatataatca gaaacataaa actggtccaa tgccataagt cgaccaaaat aatagaaggc
     1741 aaagccaatt ttttatccgc tgtccaagtc ggaccggcaa ttccgattaa accgagaacc
     1801 tggttgataa aaccgtagtt atagtcatac atcagtcgcc acatcgtacc gatggcaacc
     1861 ggcggaatca gaagggggat caggaaaacc gtacggtata accccaccaa atgatgactg
     1921 cgggagacca gaagggcaat caccaatgct aaaatggtct cgagaataac gacagcgata
     1981 acaaaaacga tcgtatttct cagagcgacc gggacgattt ggtcttccat tgccgtgtaa
     2041 agatgcttta atccgacaaa agtccaaacg gtctttccct gaacgatttt cacttcggag
     2101 acactcatcc gcagtaattg aaccgtagga taggctacca gaaaaatcag cacaagaatt
     2161 aagggaagaa tggtccatat cttaaaagta cgatcgataa aattactgaa gcgagttttc
     2221 caacccggcg gtttggaaga gagtgaaaaa tatgtcgaat gatccatcat gatctccaag
     2281 aaagttcatc gttcaccgta tatcactggt cccgatcgga gcgataaaat cctttcggat
     2341 tgcaattttt taagaataca aatttatttt cgaaggacga gaaggaggca gagtcaatct
     2401 tttcttttcc gcctccttct gaattattta ttcagccaaa ggcttcactt tatagcccgc
     2461 atctttcaga atcttaaata tttcttttgc ggcttccgac aatgtttctt ccggcgttgt
     2521 ttcggaaatt aacgcctgtt tcaaccggcg atcgataacc tcaatgattt gaggagtttc
     2581 caccaatcgg ggttgcgcat gaatgaaagg tgtactgtcc gccatagctt tcatccacca
     2641 accgactttc ggatcttgcg acatttcttc gtagacatcc tgtcgaaccg gaatcgcacc
     2701 ggcttgtgca tagtacagct gagcatcctt cgtcatcgcc catttgagaa atgccaacgc
     2761 cgcttccttc cgttccttag gcagattatg aggaatgctc ataacccaaa tacccgacat
     2821 cgttgcattt gagccggcgg tagcacccgg aacaggcacg gcggcaacct tgccctga
//
LOCUS       DAOMIV010000260         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_37618_length_2868_cov_4.713473, whole genome shotgun sequence.
ACCESSION   DAOMIV010000260 DAOMIV010000000
VERSION     DAOMIV010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37618_length_2868_cov_4.713473"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="PKV59_06560"
     CDS             complement(<1..1133)
                     /locus_tag="PKV59_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQP46452.1"
                     /translation="MNETRINNLLKETNGVQGLVFKSPSHIGYLTNVLFPYPDQSPFN
                     IGLVTSAGKAVLIVPAEWKGVAEDQNWSDDILYYTINEGSPSEAFCKAINKTIIKLNL
                     NGKAIAVDYPSWTVAEINRANILFQNSQWSDCDKTIRQLRMKKDQAEVDLLHQAAVIA
                     DRGIIGALNHAEGTIGNAEYTLVEFLERVRVHAIEFGSSGIGHLNVSMGKQGRVWFTP
                     VRDFSLLENGNFLRVNYTQEFHGYWNLCNRTFFIGLPRYSDDSAYRLHQNLISLCLKE
                     LRAGIKISDFCSEVIQHAKALKVELFNENEFGHGIGLSEVEWPLLTIDNPESFQAGMV
                     IVLDIISLGPNEELIQSTEMILIEENGYQRLSDFRDWDDLYCIV"
     gene            complement(1137..2033)
                     /locus_tag="PKV59_06565"
     CDS             complement(1137..2033)
                     /locus_tag="PKV59_06565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQP46453.1"
                     /translation="MKSKRVMVIDAFCDKPFQGNPCAVLPDARELSDEEMLLIAKEMN
                     LSETSFVLPSEKANFRVRYFTPRGELPFAGHPTIATSLMLAQENMIQITGNTTAISLE
                     FNIGVLPVEISSQNGKPNQAVMQLTAPVFGKTVSREKIAQCFKDIDPDDIRPDCPCQV
                     VGTGTNFLIIPLMTSSKLSNITMNRDNLMAIVDEVGVSAAYVFTKGGFSRETELSGRL
                     CDPRNAFEDPYTGSAVGAVGAYVIHYGLLTGDQIQVEQGHFIGRPGVGRLFIEHDGKE
                     IRSIKLGGSGVKVTDGFIFLEN"
     gene            complement(2030..2413)
                     /locus_tag="PKV59_06570"
     CDS             complement(2030..2413)
                     /locus_tag="PKV59_06570"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQP46454.1"
                     /translation="MSKREVIIGAKDLPFSKVIRFGELIFVSGMVGRNEDTHQIDFDI
                     KGQTKQLLQNMQKLLENCGSSLDNSIKITIYLTDMRYFSEMNEEYSKHFSTEKGFPTR
                     TCVAICSLPDKDAKIEMDVIAEVVK"
     gene            complement(2427..>2868)
                     /locus_tag="PKV59_06575"
     CDS             complement(2427..>2868)
                     /locus_tag="PKV59_06575"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQP46455.1"
                     /translation="QDVYDGIQQFAKDNNIRFAQIHTAFMGGFEPARMLIWTPDSTNP
                     DNWHHETPMEIQNLTMLLSMGGFIHLRENEEGKEEPFPAIHYIVGAAWNAPIWGGHLV
                     QGTIAKGNMEVFITEILGIDPIMEDNVNIGAPENWYIESNSSVK"
BASE COUNT          794 a          650 c          556 g          868 t
ORIGIN      
        1 acgatacaat aaaggtcatc ccaatcacga aaatcactca atctctgata accgttttct
       61 tcaattaata tcatttccgt cgattgaatt aattcttcat ttggtcccaa tgaaatgata
      121 tccaatacga ttaccatacc ggcttggaat gattctgggt tatctattgt gagcaacggc
      181 cactccactt cggaaagtcc gattccatga ccgaattcgt tttcattaaa taattcgacc
      241 ttcaaagctt ttgcgtgttg aataacttca gaacaaaaat cactgatttt tatcccggca
      301 cgtagctctt ttaagcagag cgaaattaag ttttgatgta accggtatgc cgaatcatcg
      361 gaatagcggg ggagtccgat aaaaaatgtg cgattgcaaa gattccaata gccatgaaat
      421 tcttgcgtgt agtttacacg caagaaattt ccgttttcta acaaggaaaa atcacggaca
      481 ggggtaaacc aaacacgtcc ttgcttgccc attgaaacat tcaggtggcc gatgcctgac
      541 gaaccgaact cgatagcgtg aacacgaacc ctttccaaaa attcaaccaa ggtatattct
      601 gcatttccga tagttccttc ggcatgattt aatgcaccga tgatgccccg atcggcaatc
      661 acagcggctt gatggagtaa atcaacttcc gcctgatctt ttttcatacg taattggcga
      721 atggttttat cacaatcaga ccattgtgaa ttctgaaaaa gaatatttgc ccgattgatt
      781 tcagcgactg tccaagacgg gtaatccacg gcgattgctt ttccgttcaa attcagtttg
      841 atgattgtct tgtttattgc tttacaaaaa gcctcggatg gagaaccttc gttaattgtg
      901 taatacaaga tatcatccga ccaattttga tcttccgcaa cgcctttcca ttctgccgga
      961 acaattaata ccgctttgcc tgcggaagtg accaatccga tgttgaaagg agattgatcc
     1021 ggatagggga ataaaacatt agtcaaatac ccgatatggg aaggcgattt gaaaacaagc
     1081 ccttgtacac catttgtttc ttttaataga ttattgattc gcgtttcgtt catagcttaa
     1141 ttttccaaaa aaatgaatcc atcggtaact tttactccgc tcccgccgag tttaatagat
     1201 cgaatttctt ttccgtcatg ttcaataaaa agcctgccga ctcccggtcg tccgataaaa
     1261 tggccttgct cgacttgaat ttggtcaccc gttaataacc catagtgaat tacatatgca
     1321 ccgaccgcac cgacagcgga accggtatac ggatcttcaa aagcatttct cggatcgcac
     1381 aatcgcccgc ttaattcggt ttctcgggaa aaaccgcctt tcgtaaaaac atatgcggcg
     1441 ctgactccca cttcatcaac gatcgccatt aaattatcgc gattcatcgt aatattcgag
     1501 agcttcgaag atgtcatcag aggaattatc aggaaatttg ttccggttcc gacaacttga
     1561 cagggacaat ccggccggat gtcatccgga tcgatatctt taaagcattg cgcgattttt
     1621 tctcttgaga cggtttttcc gaaaaccggt gcggtaagtt gcatgacggc ttgattcggt
     1681 ttcccgtttt gggatgaaat ttccacaggg agaacaccga tattaaactc taaagaaatt
     1741 gctgtcgtat ttcctgtaat ttgaatcata ttctcttgcg cgagcatcaa tgaggttgcg
     1801 atcgtcggat gtcccgcaaa tgggagttct cctctcggag tgaaatagcg gactcgaaaa
     1861 tttgcttttt cggaaggtaa gacaaaactt gtttcggaga ggttcatttc ttttgcgatc
     1921 agaagcattt cttcatccga caattccctt gcatcaggta aaaccgcaca ggggtttccc
     1981 tgaaaaggtt tgtcacaaaa tgcatcgatg accattactc ttttcgattt catttcacta
     2041 cctccgcaat cacatccatt tcaatcttgg cgtctttatc cggaagtgag catatcgcca
     2101 cgcaagtgcg cgtaggaaag cctttttcgg ttgaaaagtg ttttgaatat tcttcattca
     2161 tttcggagaa ataccgcata tcggttaaat agatcgttat ttttatcgaa ttgtccaaag
     2221 aagatccgca attttccaac aatttttgca tattttgtaa taattgcttg gtttgtcctt
     2281 ttatatcgaa atcaatttga tgagtatcct cattccgccc gaccattccg gaaacaaaaa
     2341 tcaactctcc gaaacggata acttttgaaa aaggcaaatc ttttgccccg ataatgactt
     2401 ctcgtttgct catattccct tttctgctat tttacggaag aatttgattc gatataccaa
     2461 ttttcgggcg ctccgatatt gacattatcc tccataatcg gatcgatacc gagaatttcg
     2521 gtaataaaga cttccatatt cccttttgca atcgtacctt ggaccagatg tccgccccaa
     2581 atcggagcat tccatgccgc tccgacaata taatggatcg caggaaacgg ctcttctttc
     2641 ccctcttcat tctcccgtaa atgtataaaa cctcccatgc tgagcagcat ggtaagattt
     2701 tgaatttcca tcggtgtctc gtggtgccaa ttatcgggat ttgtcgaatc gggtgtccaa
     2761 atcagcatgc gagccggttc gaatccgccc ataaaagcgg tatgaatttg ggcgaatcgg
     2821 atattattgt cttttgcaaa ttgctgtatt ccgtcataaa catcctga
//
LOCUS       DAOMIV010000261         2851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_37926_length_2851_cov_4.255007, whole genome shotgun sequence.
ACCESSION   DAOMIV010000261 DAOMIV010000000
VERSION     DAOMIV010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37926_length_2851_cov_4.255007"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..862
                     /locus_tag="PKV59_06580"
     CDS             29..862
                     /locus_tag="PKV59_06580"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQP46456.1"
                     /translation="MKTYEYADYVKVAEIIKEKIKTPIELALVLGSGLGGLADEVKHP
                     LIIDYRDLPGWPLSTVEGHKGRLVIGELAGRMVLVQQGRVHFYEGYSPAEITFPIRVM
                     QCLKIPSLIVTNAAGGVNPSFSAGDVMLITDHIGFVSMSGFNPLNGRNFDQFGPRFPD
                     MSQAYDREYGSLVRKAAIRTGIDLKEGVYTWLSGPSFETPAEIRFLRTVGTDAVGMST
                     VPEVIVARHGGMRVLGLSGITNKCSDDGEMKADHQEVLQIADIIGPKIIRILYEILPD
                     M"
     gene            881..1576
                     /locus_tag="PKV59_06585"
     CDS             881..1576
                     /locus_tag="PKV59_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46457.1"
                     /translation="MQSLLNFLVNYQNWIYTVLLITAVIYGRKLIRAIIEWRSTIFGL
                     EREFAQHKLNTSLAMVILSVLLIIAEFICVNFIISALPQATGIQSLPATTDGVDLNIV
                     ATSGAGVESGSPNASSAVAQNVTGGGCIPGQIEWIAPKPGEEISGIYNLMATVNILDM
                     GFFKYDYASLSDPTDWHAISAGNMPVVEGKLGVLATSEIPNGDYILRLTVVNKANQQL
                     TPCDVAIRILNKE"
     gene            1635..2174
                     /locus_tag="PKV59_06590"
     CDS             1635..2174
                     /locus_tag="PKV59_06590"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQP46458.1"
                     /translation="MAEIEIRMASMADIPLITKIDLDFESNYVWKSQMIDDLDSYTIV
                     FQNIKLPKTVHVPFQGGGEEAIEKMVKRQEVIVAQYDNQVAGFVRLEQDENTNRILLK
                     TGGVKPDYRRKGIGSALLKVIQENAVKVGIPRLVCTVQAKNDPAIHFLLSKGFQFCGY
                     QDFYFQNMEIALFFAKNIR"
     gene            2187..>2851
                     /locus_tag="PKV59_06595"
     CDS             2187..>2851
                     /locus_tag="PKV59_06595"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HQP46459.1"
                     /translation="MINWILPIIRTISEILTASIAIASFSLLLFSLFFIQKERLARVF
                     SLILLALSVIYTADALSLTTLSDQNQLLWQQIHWSGIIFLPATMIHFSQVILQMTGKT
                     IRKLRKKMTAVTYIFSVIFLVLLWKNQLFYGTRRVGNLGISMIPTEKLNYFWIWLFVL
                     LSAMLINFYHAFKRTRTPTSRRRMLYLIVSALGVLVGTFPLMIYGSRFLLRHELGFWL
                     VNL"
BASE COUNT          776 a          544 c          694 g          837 t
ORIGIN      
        1 aattagaagg ggaaaaggag atgaatccat gaaaacttat gaatatgccg attacgtaaa
       61 agtcgccgaa ataatcaaag aaaaaataaa aacgcccatt gaacttgctt tggttctcgg
      121 ctcgggattg ggcggtttgg cggatgaagt gaagcatccg ctgattatcg attatcgcga
      181 tcttcccggt tggccgcttt cgaccgtgga aggacataag ggccggcttg tgatcggaga
      241 gcttgccggg cggatggttc tggttcagca aggtcgggtt cacttttatg aaggatattc
      301 tccggcagaa attacctttc cgattcgtgt gatgcaatgt ctgaagatcc cgagtttgat
      361 agtgaccaat gctgccggcg gggtgaatcc gtcgttctcc gcaggcgatg tcatgttgat
      421 caccgatcat atcggcttcg tttccatgag cggattcaat ccgttgaatg gccggaattt
      481 tgatcaattc ggaccccgat tcccggacat gtcacaagcg tatgaccgag aatatggctc
      541 actcgtaaga aaagccgcca ttcgaacagg aatagatctc aaagaaggtg tttatacttg
      601 gttgagcggg ccttcctttg agacaccggc cgaaatacgt ttcctccgaa ccgtggggac
      661 ggatgcggta ggtatgtcca cggtacccga agtaattgtg gcacggcatg gcggaatgcg
      721 ggtgttaggg ctttccggca taaccaataa atgcagcgat gacggagaaa tgaaagccga
      781 tcatcaagag gttttacaga tcgccgatat tatcggtccc aaaatcatcc gaattttata
      841 cgaaattctc cccgatatgt gatgaccgga aaagaaatca atgcaatcct tgttgaactt
      901 tttagtgaat tatcagaatt ggatttatac cgttttgtta atcactgcgg taatttacgg
      961 aaggaagctg attcgtgcga ttatcgaatg gcgttcgaca attttcggat tggaaagaga
     1021 gtttgcccag cataaattaa atacttcgtt ggcaatggtc atcctttcgg ttttgttaat
     1081 cattgccgaa tttatctgtg tgaattttat tatttcggct cttccgcaag caacgggaat
     1141 ccaatcgctg ccggcgacca cggatggcgt cgatttgaat attgtggcaa cgagcggcgc
     1201 gggcgtcgaa tcgggttcgc cgaatgcttc ttccgctgtc gctcagaatg ttaccggcgg
     1261 cggatgtatt cccggccaaa tcgaatggat cgcaccgaaa cccggcgaag aaatatcggg
     1321 tatttataat ttgatggcaa cggtcaatat tctggatatg ggttttttca aatatgatta
     1381 tgcctctttg agtgatccta ccgattggca cgctatttcc gcggggaata tgccggtcgt
     1441 tgaaggaaaa ttaggtgtct tggcgaccag tgaaattccg aacggtgatt atattttgcg
     1501 attgacggtt gtaaataaag cgaatcaaca attaacacct tgcgatgttg ccattcggat
     1561 tttaaataaa gagtaataac tcaataatag gattaaaatc aaagaaacga aaaggttaag
     1621 gatatcgata aatcatggct gaaatagaaa tacggatggc gtcgatggca gatatcccgt
     1681 tgattacgaa aattgacttg gattttgaaa gcaattatgt atggaaatcg caaatgatcg
     1741 atgatttgga ttcatatacg attgtctttc agaatatcaa gttgccgaag accgtccatg
     1801 ttcctttcca aggaggcgga gaagaggcta tcgaaaaaat ggtcaagcgt caggaagtca
     1861 ttgtcgcaca atatgacaac caagtggccg gttttgtgcg tttggaacaa gatgaaaata
     1921 cgaatcgaat tttattaaaa acaggaggtg tgaaaccgga ttatcgcaga aaaggaatag
     1981 gatctgcact cttgaaggtg attcaggaaa acgcggtaaa agtgggaatt ccccgtcttg
     2041 tttgtaccgt tcaggcgaaa aatgatcctg cgattcattt tctgctttca aaagggtttc
     2101 aattttgcgg gtatcaggat ttttattttc aaaatatgga gattgcgtta ttctttgcaa
     2161 aaaatatccg gtagcctgcg aacgatatga ttaattggat tttgcctatc attcgaacaa
     2221 tcagtgaaat tttgaccgca tccattgcga ttgcgtcatt ttcacttttg ttattttctt
     2281 tattttttat tcaaaaagaa cgacttgcac gggttttctc attaattctt ttagctttaa
     2341 gcgtgattta tacagctgat gcgctttcgc tgacgacatt atccgaccaa aatcaattgc
     2401 tttggcagca aatccattgg tcggggatta tctttttacc ggcgacgatg attcattttt
     2461 cccaagtaat tttacagatg acgggaaaaa caatccggaa gctgaggaaa aagatgaccg
     2521 ccgtcactta tattttctcg gtgatttttt tggtcttgct gtggaagaat caactttttt
     2581 acggaacacg acgggtcgga aatcttggaa tttccatgat cccgacggag aaattaaatt
     2641 atttttggat ttggctgttt gtccttttga gtgcgatgct gatcaatttt tatcatgctt
     2701 ttaagcggac ccgaacaccg accagccgtc gaaggatgct ctatctgatt gtcagcgcgt
     2761 taggagtttt ggtcgggaca tttcctttga tgatttacgg atcccgcttt ctgctgcggc
     2821 atgaactcgg attttggctg gtcaatttaa t
//
LOCUS       DAOMIV010000262         2849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_37966_length_2849_cov_4.222262, whole genome shotgun sequence.
ACCESSION   DAOMIV010000262 DAOMIV010000000
VERSION     DAOMIV010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37966_length_2849_cov_4.222262"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PKV59_06600"
     CDS             complement(<1..985)
                     /locus_tag="PKV59_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46460.1"
                     /translation="MESSTLPPESEEITDNDLMNAVRAGRPQFVDTRAQIVRLLTEVG
                     QAKITAEEFYQKVSKLEMPPDKELRHALMEVKKDIGNYLGEEGIRKVFGEIYPSGATR
                     VEATILAHRKAVVMETIKLAIVAYREKVKAATGEYPKMTFYYGEVGGWSKETFEELKF
                     AGDIDFNFLSGDLDQAMELKKIFDEMIMKRYGRTPEELDVPCTVHGMATGEVYVGKHG
                     QSFAEDVTNVAWEIDLENASPDMDLKAKSVPFKEALETMVLEARMAAVNNAISDLKTS
                     KWPYQPGISLEMIRHFEHDIAGKNVFTDLESFVKAAKYTDRSFSFLIKELGE"
     gene            complement(1323..2366)
                     /locus_tag="PKV59_06605"
     CDS             complement(1323..2366)
                     /locus_tag="PKV59_06605"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HQP46461.1"
                     /translation="MKNELKLESIKRELEQLRRVKIDNLINVSNAEWYALFKDEIRNS
                     IAIEGVFANRTDLIDVLENNKRTDKHKAASILGYFESASAMYEYAVNQYKEKEFFLRM
                     SDIKQIHTLLMRHEKEMGFYIGDLGEFRKTRAEVAQSTFTPISEFYVRPAMELFIKWI
                     NHHLHKNTYNSIELAAISHVWFESIHPYRDGNGRVGRILLSYIFIGCGLINIAIKGVS
                     KKDRNHYYDALEQADDAFEDIHRKMEQGEKFDVAGVDQLIKLQVFDEIKTIILDRLND
                     SVGRLKEIDIHNINKEIELSLSELARIYNYSSDYLRNLINRGKLKGRKKGKTWYVRVR
                     DMAAYADEAGNGA"
BASE COUNT          794 a          693 c          500 g          862 t
ORIGIN      
        1 cctctccgag ttctttaata agaaaactaa aactcctgtc cgtgtacttg gccgctttaa
       61 caaacgattc taaatccgta aaaacattct tcccggcaat atcatgctca aaatggcgga
      121 tcatctccaa actaatcccc ggctgatagg gccatttcga tgtttttaaa tcggaaatgg
      181 cattattcac tgcggccatt cttgcctcca ggaccatcgt ttcaagcgct tctttaaacg
      241 ggaccgattt ggcttttagg tccatatcag gcgaagcgtt ctctaaatca atttcccagg
      301 ccacgttcgt tacatcttcc gcaaatgact ggccatgctt gccgacatac acttcgcccg
      361 tcgccatgcc atgaaccgta cacgggacat ccagttcctc cggcgtacgc ccataacgct
      421 tcatgatcat ttcatcaaag atcttcttaa gttccatggc ctgatcaaga tccccggaga
      481 ggaaattaaa gtcaatatcc ccggcaaatt taagctcttc aaatgtctcc tttgaccatc
      541 cgccaacttc accataataa aaagtcattt taggatattc cccggttgcc gctttaactt
      601 tttccctgta agcaacaatc gccagcttga tcgtttccat aacaacagct tttcgatggg
      661 ccaaaattgt tgcttcaacg cgggttgctc ccgacggata aatttctcca aacacctttc
      721 ttatcccttc ttcacctaag tagttaccaa tatctttttt cacttccatg agggcatgac
      781 ggagctcttt gtccggcggc atttccaact tcgatacttt ctgataaaac tcttctgccg
      841 ttattttagc ttggcctact tccgtcaaca gtctgacaat ttgagctcgc gtatcgacaa
      901 actgcggccg gccggcacgg acagcattca taagatcgtt atctgtgatt tcttcggatt
      961 ctggcgggag ggtggaactt tccacagaag tgacattccc cgcaggctca gcgaatacaa
     1021 actgagcgca gagaaggaaa gtaaataagc tgacaacgat ccttaaaggc tgttgcagag
     1081 ggaaccgact gaatagggtc atatcatttc tctgttaaga taggggacgg cagcgcccta
     1141 tctccgtcct ccaacaaaaa acccgggcct atgactcccg ggtctataat tagctgcttg
     1201 ttttgtgttt ccgactgtga tcgttttttc cgttcatgac aaattggcca attgcattac
     1261 gttacaaagt cgggttattt tatcaccaaa cccgccgaat taccatgaca tttgttgcac
     1321 gactatgctc cgttccccgc ctcatcagca tacgccgcca tatcccggac gcgcacatac
     1381 cacgtcttac cctttttacg ccccttcaat ttccctcggt tgattaaatt ccgaagatag
     1441 tccgacgaat aattataaat acgggccaat tcgctcaatg ataattcaat ttctttgttg
     1501 atattatgaa tatcgatttc ctttaaacgc ccgactgaat cattaagacg gtcgaggatg
     1561 attgtcttaa tttcatcaaa gacttgtaat ttaattaatt ggtcgacgcc cgccacgtca
     1621 aacttttctc cttgctccat tttgcgatgt atatcctcaa acgcatcatc cgcttgttcc
     1681 aatgcgtcat aataatgatt acgatctttc ttagaaacac ctttaatcgc aatgttaata
     1741 agaccgcatc ctataaaaat atacgacaat aatattcttc cgacccgtcc attaccatcc
     1801 cgataaggat gaatggattc aaaccaaacg tgtgatatag cagccaattc aatagaatta
     1861 taagtattct tgtgcaaatg gtgattaatc cacttgatga ataattccat tgcgggacgc
     1921 acataaaatt cactaatcgg agtaaatgtt gattgcgcaa cctccgccct ggtcttgcgg
     1981 aattcaccca aatccccgat ataaaatccc atctcttttt cgtggcgcat caaaagcgta
     2041 tggatctgct taatatcaga catacgcaaa aaaaattctt tttccttata ttggttaaca
     2101 gcgtactcat acatcgcaga tgccgattca aaataaccca aaatggatgc tgctttgtgt
     2161 ttgtcagttc ttttattgtt ttctaaaaca tcgattaaat ctgttcgatt ggcaaagacg
     2221 ccctctatcg caatagaatt acgaatctca tctttgaaca atgcatacca ctcagcattt
     2281 gacacattaa ttaagttatc aattttgaca cggcgtaatt gttcgagctc cctttttatt
     2341 gattctaatt ttaattcatt tttcatttaa tcctccatcg aacttaccga accatacact
     2401 taaatggact atttataatc aattctatta caatgtatta tgcaaacttc tgctatcgaa
     2461 cctagccaca tggttcgata gtaatatacc atattccggg gttaacgcaa cttatttttt
     2521 aatatttagc gaacttaccg aaccataagc tatttgtaac ttatttatat acataaagtt
     2581 atgtattcca gatataccga accttactat atggttcgat atagtcgggc tatgagggga
     2641 ttcttctttc agatattgca tgttaaatat tataactcag agaggccgga aagaataaaa
     2701 gtgtatccaa gcggtatcca agggaatttg gtgggataaa aaaagggcca cagaataatg
     2761 ctaactctat ggccctatat tggttgcggg ggaccgattt cgctcgcccc tccggggctc
     2821 ccggaatcga ttcttcccca atattcaaa
//
LOCUS       DAOMIV010000263         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_38320_length_2831_cov_3.846902, whole genome shotgun sequence.
ACCESSION   DAOMIV010000263 DAOMIV010000000
VERSION     DAOMIV010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38320_length_2831_cov_3.846902"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            347..1057
                     /locus_tag="PKV59_06610"
     CDS             347..1057
                     /locus_tag="PKV59_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46462.1"
                     /translation="MKKNLLIVFFLLIFLEVTAVYAENPRVQCGNGTVTIKMSVPSTN
                     VRCLTVMNSEKAAEGKSLSEARISSAAIVIDDYKVEGKFSPEVTAFSMNGLSNVNSEI
                     YQKAVDLTTMINKINGGEGINAVTAPIPFLPYQDKTQLFVAMPEIINFISGTAIRFIT
                     AYGDSGAMVDNGNLLYTVQGISQDGKYYLSITIPISNSQIASPINPAGFDWNSLPPES
                     WKPSLTELDEMAKSVSLQ"
     gene            1115..2728
                     /locus_tag="PKV59_06615"
     CDS             1115..2728
                     /locus_tag="PKV59_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46463.1"
                     /translation="MFAIVLSSGAIARGIDTDWGAVLIHKVPVEITEYQFAEGKIYRP
                     ITAQSLNERPAILLINALNPDNEALSSLASELARRGFVALSIDLSIHGDSVESTRNNL
                     MEILSAGFQFLESQSFVLKDYIGLAAYSIPTLDQPVEKFIQENFRSVVFIASAPNLLS
                     DYQNVENEPYLLRINSTFDEYQNYRIDQTASPDEQFFAIHPFAPIDSKVISQTLNHFH
                     QTLKISNDSPLWFDADKQLSTLREISLSVGFVALLLTLILLSQMIGKKHLLEMPSEYS
                     KKDINPKPFSFSRQIGGSFCGFLLLLITYWIFLRFSQRAENQLNVSDGIVIWAGTASF
                     IMLVSGFFSKKGTLRMQRLKLIDLFLSFIRGIVICLILYGIVWIFSRVFMIEFRFIVP
                     GLRPFSDINKTRLSLYSLFFCLFFWLHATWPKQKNFLFDNFIPIGAFLFFLAVQFFPV
                     LFGGTPGLEFLTRQFSAVAGLPSVTTDSFFTLCLGAMTDTVMYSFIIFSLLLIIQNRM
                     KRSINNNIISAIICGCVCSWLLYNGTIII"
BASE COUNT          826 a          557 c          549 g          899 t
ORIGIN      
        1 atgccggatt agggaaaaaa ctcattaata aagctattca aatcgcaaaa aacaacaact
       61 tcaagaaact cgccgtcatt tcggcaatcg gaacgcgaaa atattacgaa tccagaggtt
      121 tttcccgcgg agaatattat atggttatgg atctaactaa caaataaatt tgaggttcaa
      181 atccaaataa tcgatatcat taaaatggaa aaatggaatt ttctgagaaa tgtttttgta
      241 aaggttttta gcgattaata ttgataagga aattttttta tcatcaacaa tctttcaata
      301 attgtaaaaa aataggcaat cgtgaaaaaa ttgaaaggag cttaccatga agaaaaacct
      361 attgattgtt ttctttctgt tgatcttttt ggaagtgacg gcggtttatg ccgaaaatcc
      421 tcgggtgcaa tgcggaaacg ggacggtgac gataaaaatg tcggttccat ctaccaatgt
      481 acgttgttta accgttatga atagtgaaaa ggcagccgaa gggaaatcat tgagcgaagc
      541 aagaatctcg agcgcagcca ttgtgatcga tgattacaaa gtcgaaggaa aattctcacc
      601 ggaagttacc gctttttcga tgaacggatt atccaatgtg aattccgaaa tttatcaaaa
      661 agccgtggat ttgacaacca tgatcaataa aattaacgga ggggaaggaa tcaatgcggt
      721 tacagcaccg atcccgttcc ttccatatca agataaaacc caattatttg tggcgatgcc
      781 ggaaataatt aatttcatat cgggaacggc aatccgtttt attaccgctt acggagacag
      841 tggagcgatg gttgacaatg gtaatttgtt atatacggtt cagggaatct ctcaagacgg
      901 aaaatattat ttaagtatca ccatcccgat cagtaacagt caaattgcaa gtccgattaa
      961 tccggcgggg tttgattgga attctcttcc tccggagagt tggaaacctt ctctgacgga
     1021 actggatgag atggcaaaaa gtgtttcgct gcaatgatca ttaatcgata gcgtgaagtc
     1081 tgtccggaat tcccccgcta aattatttct gtatttgttt gcaatcgtcc tgtcttccgg
     1141 tgcgatcgca agaggaatcg atacagattg gggagctgtt ttaattcaca aagtcccggt
     1201 tgagattacg gaatatcagt ttgcggaagg gaaaatctac cgaccgatta ccgctcaatc
     1261 attgaatgag cgcccagcca ttctcttgat caatgcgctg aatccggata atgaagcact
     1321 ttcttcgctg gcttctgagc tggcacgacg cggatttgtc gctttatcaa tcgatctttc
     1381 aattcacggt gattctgtcg aatcaacccg gaataacttg atggaaattc tctcagccgg
     1441 attccaattt ttagaatccc aatctttcgt tttgaaagat tatatcggtc ttgcggctta
     1501 ttcgatcccg acattagatc aaccggttga aaaattcatc caagagaatt tccgatcggt
     1561 tgtatttatc gcatccgcac cgaatttatt atcggattat caaaatgttg aaaacgaacc
     1621 gtatttactt cgaattaatt caacgtttga tgaatatcaa aattatcgga tcgatcaaac
     1681 cgcatctccg gatgagcaat tttttgccat tcatcctttt gctccgattg attcaaaggt
     1741 tatctcccaa acactcaatc atttccatca aacattaaaa atatcaaatg actcacccct
     1801 ttggtttgat gccgataaac aattaagtac cttacgtgaa atttcattat cggtcggatt
     1861 cgtcgcactt ctattaacac tcatcctatt aagtcaaatg atcggcaaaa aacatcttct
     1921 cgaaatgccc tcagaatact cgaaaaaaga tataaatccg aaaccgtttt ctttttcacg
     1981 gcaaatcggc ggctccttct gcggtttttt attacttctt atcacatatt ggattttcct
     2041 ccgattttct caaagagcag agaatcagct gaatgtctct gatgggatcg taatatgggc
     2101 gggaaccgct tcgtttataa tgctcgtttc cggattcttt tcaaaaaaag ggacattgag
     2161 gatgcaacgg ttaaaactta tcgacttatt cctctctttc atccggggta ttgtgatatg
     2221 tctgatcctc tatggtatcg tttggatatt ttcgagagtt ttcatgattg aattcagatt
     2281 tatagttccg ggcttacgcc ctttttcgga tattaataaa acgcgcctga gcctatactc
     2341 tcttttcttt tgtctatttt tttggttaca cgcaacatgg ccaaaacaaa aaaacttctt
     2401 gtttgataat ttcattccta ttggagcttt tctgtttttc ctcgccgttc aattttttcc
     2461 tgttttattt gggggcactc cgggcttgga gttcttgacc cgacagtttt ctgctgtagc
     2521 cgggttgccc tctgtcacaa ccgattcttt cttcacgctt tgtttgggtg cgatgacaga
     2581 taccgttatg tacagtttta tcatttttag tttattattg attatacaga atcggatgaa
     2641 acgatcgatt aacaataata ttatctcagc tataatatgc ggatgtgtct gttcttggct
     2701 tttgtacaac ggaaccatca tcatttagaa acgaaagaag tctcagaatg aaaaaaatac
     2761 ccttattaat actttgcctt gttataacgg ccggtttttt cggtttttgc tgcgtcaacc
     2821 aaattcaaca a
//
LOCUS       DAOMIV010000264         2827 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_38402_length_2827_cov_3.575036, whole genome shotgun sequence.
ACCESSION   DAOMIV010000264 DAOMIV010000000
VERSION     DAOMIV010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38402_length_2827_cov_3.575036"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..460
                     /locus_tag="PKV59_06620"
     CDS             158..460
                     /locus_tag="PKV59_06620"
                     /inference="COORDINATES: protein motif:HMM:NF019491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HQP46464.1"
                     /translation="MFIHIVTWKLKENAGDRSKEENIRLILEKINTLPAIIPQVKSLK
                     AGADVLHTPTSYDFGLVGEFENQADFLIYRDHPEHVKVARFVSDLTSDRVSLDFIV"
     gene            complement(465..914)
                     /locus_tag="PKV59_06625"
     CDS             complement(465..914)
                     /locus_tag="PKV59_06625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQP46465.1"
                     /translation="MTEQEIKPYYHKAQYYETDQMGIIHNSNYLRWFEECRTDYMDQI
                     GLPYDAMEKAGIISVVLVSHCEYKHPLHYEETAEIRAEIVHFNGYKMTVGYIVKNAET
                     GTVCATGYTQHGMLSKDLSKPVHLEKTFPLAYQILSDHCKTQFEKEG"
     gene            complement(932..1555)
                     /locus_tag="PKV59_06630"
     CDS             complement(932..1555)
                     /locus_tag="PKV59_06630"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQP46466.1"
                     /translation="MKTDHSGKHYAESKEAEKILVWAQSSNPGRWIGHSKYTGLACRL
                     IAEHCPDMDPEKAYTAGLLHDIGRYVGVVRGRHQIEGYRICMERGWDANARICLTHSQ
                     VLKDLTKSLDYWDISEEDFEFFNRYIQGIEFDDYDLLVQLGDCLALPSGFCIIEKRMI
                     DVARRYGINKDSVERWNKIFELKDYFNRKTGSNIYRYLPNIEENTIL"
     gene            complement(1552..2016)
                     /locus_tag="PKV59_06635"
     CDS             complement(1552..2016)
                     /locus_tag="PKV59_06635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQP46467.1"
                     /translation="MTYRIKAYKRRVQYHETDRMGIMHNSNYYRWFEEARIDYMDQTK
                     YPLKVIEGMGIFLPVAASSAEFLSPAEFAEDFYVVSEMVHFTGYKMTINFKIIHANSR
                     SVCTTGQIKYGVISKEGKPILLQRQFPEVYEDWQAVLNLPSVIDELTDPIRI"
     gene            complement(2257..>2827)
                     /locus_tag="PKV59_06640"
     CDS             complement(2257..>2827)
                     /locus_tag="PKV59_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQP46468.1"
                     /translation="YAHAVMRKITDYKLAFWEKALEKLGENVLIVSEADDLATQNGPL
                     ISPRLYKEMIHPYHKELYQFIHSKAKNEIFIFYHTCGSCKAFIPFLIEEGVNILNPVQ
                     VSAKDMGTAELKREFGKDLTFWGGGVDTQKILPFGTPDQIREEVKHRIDDLAKDGGFV
                     FAAVHNVQSDVPVENFMAMWETLRQYGKY"
BASE COUNT          781 a          595 c          579 g          872 t
ORIGIN      
        1 tttcattacg cttgatttta gcatattttg agttcggaat ctgcgccgga gattttattt
       61 ttgcggtatt atataaaaat cccgcaaaaa ttctatctga atcgagtgaa tccaaaaaag
      121 cggcgagaaa aaacgaaaag atcagaaaga ggaaaatatg tttattcaca ttgttacatg
      181 gaagctaaaa gaaaatgccg gagatcgttc aaaagaagaa aatatccgat taattttgga
      241 aaaaatcaac accttaccgg cgatcattcc ccaggtaaaa tctttgaaag ccggcgcgga
      301 tgtgttgcat acgccaactt cttatgattt tgggttggta ggcgaatttg aaaaccaagc
      361 ggatttcttg atctatcggg atcatccgga acatgtgaaa gtcgctcgat ttgtatcaga
      421 tcttacatct gacagggttt cgctcgattt tattgtttaa tcctttatcc ctctttttcg
      481 aattgcgttt tgcaatgatc ggataggatt tgatacgcta gcgggaatgt tttttccaaa
      541 tgaaccggtt tcgacaggtc ttttgaaagc atgccatgtt gggtataacc ggtagcacaa
      601 acggtaccgg tttcggcgtt tttaacgata tacccgacgg tcattttata tccgttgaaa
      661 tgaacaattt cagcccggat ttcagccgtt tcttcataat gaagcggatg tttgtattcg
      721 cagtgagaca ccagtaccac agaaataatt ccggcttttt ccattgcatc atagggtaac
      781 ccgatctgat ccatgtaatc cgtccggcat tcttcaaacc aacgcaaata attcgaattg
      841 tgaatgatcc ccatctgatc ggtttcatag tattgggctt tatgataata aggttttatt
      901 tcttgttcgg tcaattttct catctcccat cttataggat tgtgttttct tcaatattcg
      961 gaagatatct ataaatgttt gaacccgttt ttcgattgaa atagtccttt aattcaaaga
     1021 ttttattcca ccgctcaaca ctgtctttat tgataccata tctcctggca acatcgatca
     1081 ttcttttttc gattatacag aaacccgagg ggagagccag acaatcaccg agctgaacca
     1141 gcaaatcata gtcatcaaat tcgatacctt gaatgtaacg attgaaaaat tcaaagtctt
     1201 cttcggaaat atcccaataa tccaacgatt tggtgagatc ttttaaaact tgggaatgag
     1261 ttaaacagat tcgggcgttg gcatcccaac ctctctccat gcatatgcga tagccctcaa
     1321 tttggtgtct gccgcgaaca acacccacat accgtccgat atcatgcagt aaacctgcgg
     1381 tatatgcctt ttcggggtcc atatccggac aatgttccgc aatcaaacga catgccagtc
     1441 cggtatattt tgaatgacca atccatcttc ccggattgga gctttgcgcc cacaccagga
     1501 ttttttcggc ttctttcgat tccgcgtaat gttttcccga atgatccgtt ttcatattcg
     1561 aatcgggtct gttaactcat cgatgactga gggaagattc aacaccgcct gccagtcttc
     1621 gtaaacttcg ggaaattgcc tttgtaacaa aatgggtttg ccctcttttg agatgacgcc
     1681 gtattttatc tggccggtcg tgcatacgga gcgagaattt gcatggatta ttttaaaatt
     1741 gatagtcatc ttatatccag taaaatgaac catttcgcta actacataaa aatcctcggc
     1801 aaattctgcc ggagataaaa attctgcgga agacgcggcg accggaagga aaattcccat
     1861 tccttcgatc actttcagcg gatatttggt ttgatccata taatctattc tggcttcctc
     1921 aaaccaccga taataattcg aattgtgcat gatacccatg cggtctgttt catgatattg
     1981 tacgcgacgt ttataagctt ttatcctgta agtcatgatt gatttccctg atttgtccga
     2041 ttcggcaagg aataaaaaat tcaatgcgat cggattattt taaccacagg tttaaattat
     2101 aacgcccaaa aaagggacaa agaatttatc tgtctcaaag cagccgaagg ataggaggtt
     2161 tgatgtcatt ttcggtaagc ccgagccggg gaagatcagc gggctcaccg cttgggtatt
     2221 ttttgaaaat tttgatgaag gaagaaggat ctgtattcaa tattttccgt attgccggag
     2281 cgtctcccac attgccatga agttttcaac cggtacatca ctttggacgt tatgaacggc
     2341 tgcgaatacg aagcccccgt ctttggcaag gtcgtcaatc cggtgtttga cttcttcacg
     2401 gatttgatcg ggtgttccga aaggcaagat tttttgagta tcaacgcctc cgccccagaa
     2461 agtgagatct ttgccgaatt ctcgtttcag ttcagcggtg cccatatctt ttgcactcac
     2521 ctgaacagga ttgagaatat tcacgccttc ttcgattagg aacgggataa acgctttgca
     2581 ggatccgcag gtatgataga aaatgaaaat ctcatttttt gcttttgaat ggatgaattg
     2641 gtagagttcc ttatgataag gatgaatcat ctctttatat agacgagggg aaatcagcgg
     2701 tccgttttga gtggcgaggt catccgcttc tgagacaatg aggacattct cacccaattt
     2761 ttccaacgcc ttttcccaga aagccaattt ataatccgtg attttccgca tgaccgcatg
     2821 tgcatat
//
LOCUS       DAOMIV010000265         2821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_38524_length_2821_cov_3.601952, whole genome shotgun sequence.
ACCESSION   DAOMIV010000265 DAOMIV010000000
VERSION     DAOMIV010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38524_length_2821_cov_3.601952"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /gene="rimM"
                     /locus_tag="PKV59_06645"
     CDS             <1..342
                     /gene="rimM"
                     /locus_tag="PKV59_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQP46469.1"
                     /translation="RSMPPFFLVQFVEIDSPEKASELRNQFVFVKRIDLPKLPKGRYY
                     FHQLIGLEVFEINGTRIGKVKEILETGANDVYVIEKEDQTEVLAAGIPEVIREINLEE
                     KKMVVNLPEWI"
     gene            349..1452
                     /gene="dprA"
                     /locus_tag="PKV59_06650"
     CDS             349..1452
                     /gene="dprA"
                     /locus_tag="PKV59_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQP46470.1"
                     /translation="MPDQLAYWVAFNHIKGFGSVRFQLLLNYFSSLEVAWNATPIELA
                     AAGVHERQIKAILDFREKNSPEDLLNKIQRSGISVVTRMDDNYPALLKLIATPPPVLY
                     FKGTLPEQDKKCIAIVGTRRMTAYGSEMAREISAFLASRGVVIVSGLARGIDSVAHQA
                     ALNCGGETAAVLGSGVDVIYPPEHRLLAKNITESGALISDYPPGTAPERTNFPPRNRI
                     ISGMCSACIIIEAGEKSGSLITARFAAEQGREVFVLPGNLNAPQSIGTNRLIRDGARP
                     LLQKEELLEFIESYESVNPVALNKKIQISFEDPLEQKILDLIDVEALHVDEISRRLGL
                     TAGKASSILTMLELKGFVQETAPQTYRKYLSLF"
     gene            1592..2677
                     /locus_tag="PKV59_06655"
     CDS             1592..2677
                     /locus_tag="PKV59_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQP46471.1"
                     /translation="MENDFYAVIMAGGGGTRLWPLSTKDHPKQMLKFAQDETMFQMTV
                     SRLTEKIPFENILVVTTEDQAAKLKQQCNNIPEENYLIEPLPRGTASVIGLAAIHLEK
                     RMPGSIMAVIPSDHFVLNEKQFLALLENGYRAAKDQHLVTLGVKPSFPSTGMGYIEQG
                     EKIPVGYDLYHVGQFREKPDKETAEYFVQSGKFWWNAGIFVWSSRRILNEIAMFLPDL
                     SKKLDIINATIDRPDYTDTMEKTWRLIEPATIDYGVLEKTTDVVFLPTYDLGWNDIGS
                     WESLFDVIPPDENGNVIINCKFHSIDSDGVLACSENKEKMIVSIGVKNMIIIETEKAV
                     LVCPRSETQRVREIVDFLKKNNLTAYL"
     gene            2687..>2821
                     /locus_tag="PKV59_06660"
     CDS             2687..>2821
                     /locus_tag="PKV59_06660"
                     /inference="COORDINATES: protein motif:HMM:NF039193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HQP46472.1"
                     /translation="MEAYCLKCKQKREIQNPVAEYTANERPITKGTCPVCNSKLSLIG
                     K"
BASE COUNT          864 a          548 c          669 g          740 t
ORIGIN      
        1 cgttcaatgc ctcccttctt tttggttcaa tttgttgaaa tcgacagtcc cgaaaaagct
       61 tcggagttac gcaatcaatt tgtctttgtg aaaagaatcg atttgccgaa actccccaaa
      121 ggcagatatt attttcatca attaatcggt ctggaagtgt ttgagataaa cggtacgcgg
      181 atcggtaaag tgaaggaaat tttggagacc ggtgcaaacg atgtatatgt catcgaaaaa
      241 gaggatcaaa cagaagtgct ggctgccggc attccggaag tgattcggga aatcaatctt
      301 gaagaaaaga aaatggtagt taatctgccg gaatggatat aaatcaaaat gcccgaccaa
      361 ttggcttatt gggtggcatt caatcatatt aaaggttttg gttcggttcg ttttcaactt
      421 ttattaaatt acttttcatc actcgaagtg gcctggaatg caacgccgat tgagttggca
      481 gccgcaggag tccatgaacg acaaatcaaa gcaattcttg attttcgtga aaaaaattct
      541 ccggaagacc tgttgaacaa aattcaaaga tcggggatat cggttgtaac ccgaatggat
      601 gacaattatc ccgctctctt gaaactcatt gcgacgcctc cgcctgttct ctattttaaa
      661 gggacactgc cggaacagga taaaaaatgc attgcaatcg tcggtacccg aagaatgact
      721 gcttacggaa gcgaaatggc acgagagatc agcgcttttt tggcatctcg tggagtcgtc
      781 atcgtttctg gattggcgcg cgggattgat agtgtcgctc atcaggctgc tttgaactgc
      841 ggcggtgaaa cagcggctgt tctcggctcc ggtgtggatg taatttatcc tccggaacac
      901 aggctcttag ccaagaacat aacggaatcc ggtgctttaa tcagcgatta tccacccgga
      961 acggctccgg aacggacgaa ttttcctccg cggaaccgga tcatttccgg aatgtgcagc
     1021 gcatgtatta ttatcgaagc gggggagaaa tccggttcct tgattacggc acgttttgcc
     1081 gccgaacagg ggcgcgaagt attcgtgtta ccggggaact taaatgcacc acagtcgatc
     1141 ggaacaaacc gcctgatccg ggacggtgct cgacctttac ttcagaaaga ggaattgttg
     1201 gagttcattg aatcttatga atccgtcaat ccggtagctc tgaataaaaa aatccagata
     1261 tcgtttgagg atccgttgga acagaaaatc ttggatttaa tcgatgtcga agcgctgcat
     1321 gtcgatgaaa tttcaagacg attgggactt accgcaggaa aggcttcatc gatattgacc
     1381 atgttggaat tgaagggatt tgtgcaggag acagccccgc agacttaccg aaaatattta
     1441 tccctttttt aatatttcat attgaacaaa aatgaatgag aatttcttca ttggagtgaa
     1501 attctgatta agccttgata aaagacggat tcaatggcat tattcataag aatttcaagc
     1561 cggataggag aaaacaggca acggagggaa aatggaaaat gacttttatg ccgtgattat
     1621 ggcaggtggc ggaggaacgc gtctctggcc gctttccaca aaagatcatc ccaaacaaat
     1681 gttaaaattt gcacaagatg agaccatgtt ccaaatgact gtctccagat tgacggaaaa
     1741 gatacctttt gagaatatat tggtcgtaac cacagaagac caggctgcga aactgaaaca
     1801 acaatgtaat aacataccgg aagaaaatta cctgattgaa cctttgcctc ggggaaccgc
     1861 cagtgtgatc ggactggcgg cgattcactt ggaaaagaga atgcccggtt caatcatggc
     1921 tgtgataccg tccgatcatt ttgttctgaa tgaaaaacaa tttttagctt tactggagaa
     1981 tggataccgg gctgcaaaag atcaacactt agtgacgttg ggggtgaaac cgtcctttcc
     2041 ttccaccgga atgggatata tcgaacaagg agaaaaaata ccggttggtt atgatttgta
     2101 ccatgtcgga caatttcgag aaaaaccgga taaagaaacc gcggaatatt ttgttcaatc
     2161 aggaaaattc tggtggaatg cgggaatatt cgtttggtca agccgccgga ttttgaatga
     2221 aatagcgatg tttcttccgg atctctcaaa aaagttggat ataatcaacg cgactatcga
     2281 tcggccggat tataccgata ccatggaaaa aacctggaga ttaattgagc cggcaaccat
     2341 cgattatggt gtcttggaaa agacaaccga tgtcgttttt cttccgactt acgatctcgg
     2401 gtggaatgat atcggatcat gggaatcttt atttgatgtg atacctcccg atgaaaacgg
     2461 gaatgtcatc ataaactgta aatttcattc gattgattcc gacggagttt tggcgtgttc
     2521 cgaaaataaa gagaaaatga ttgtatccat tggggtaaag aatatgatca tcatcgagac
     2581 ggaaaaagcc gtattggttt gtccgcgatc cgaaacacaa agagtacgtg aaattgtcga
     2641 ttttttaaag aaaaacaatt taaccgcata tttataggag aaaagtttgg aagcttattg
     2701 cctgaaatgt aaacaaaaaa gagaaattca aaatccggtc gcagaatata ccgcaaacga
     2761 gagacctatc acgaagggaa cctgccctgt ctgcaattca aaactcagtc taatcggaaa
     2821 a
//
LOCUS       DAOMIV010000266         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_38663_length_2813_cov_4.839014, whole genome shotgun sequence.
ACCESSION   DAOMIV010000266 DAOMIV010000000
VERSION     DAOMIV010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38663_length_2813_cov_4.839014"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PKV59_06665"
     CDS             complement(<1..1231)
                     /locus_tag="PKV59_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HQP46473.1"
                     /translation="MKNDRDYRPIILFGIILFFILFIGLFTPIRDKISFRVENLKNRL
                     YMLSNSPADTVFVPQGGEENISMLSQTAEGTHTPTITMTMTVPPTETAVSVTPAATAT
                     PTLNPTATITPTPLPESAHIDGVKYEDQHGIWNYCAPTNLSMALTFWGWKGNRLDAGK
                     WLKPFDKDKNVMFYEMENFVAEQTQLRSIVRSGGTPELLKKLIAAGFPILIEKGSFMQ
                     EVSGAYSWMGHYNVITGYNDATQIWTVQDSYYKPNYEVTYETLYKEWKCFNFEFMIVY
                     PGDKEAELYSLLGPYVNSDWAKQNAFEMANNQVAEAQDDESRFYAYFNRGTSQVALTD
                     YYGAAQSYDMAFSYYANLDTKTRPYRIVWYQTGPYYAYYYSGRYQDVINLANITLAST
                     NEPYLEESYYWRAMAEAA"
     gene            complement(1317..1661)
                     /locus_tag="PKV59_06670"
     CDS             complement(1317..1661)
                     /locus_tag="PKV59_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46474.1"
                     /translation="MNNKITIIEGPTPIFEATNSDWTAAIVESPANYEVVMTNLRTLN
                     GPGLIERCQSTWEQQDTMYLQFRNSIGLEQEVPIVAARCLDTEEGQKLVLWLRIKPEQ
                     IKLEVHLDEDED"
     gene            1941..2013
                     /locus_tag="PKV59_06675"
     tRNA            1941..2013
                     /locus_tag="PKV59_06675"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1974..1976,aa:Pro,seq:tgg)
     gene            2099..>2813
                     /locus_tag="PKV59_06680"
     CDS             2099..>2813
                     /locus_tag="PKV59_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQP46475.1"
                     /translation="MESTINPSSHIYLSIILPAHNEEKRLPICLEKVIAFVSSLRFPF
                     EVIVVENGSVDQTLAIATEYAKRYDWLRVLQNSKPGKGLAVRTGMLAAKGEYRFFADV
                     DFSMPIEEILNFLPPKLSDYDVAIGSREVKGAIRYNEPPIRHLTGRVFNQIVRLMTVH
                     NIQDTQCGFKCFKANAAEKIFSVQVLDGWAFDAEVLYIAQKNGFKIVEVPVRWYYDGQ
                     SKIHVLNDSVKMFQELLQIR"
BASE COUNT          792 a          596 c          577 g          848 t
ORIGIN      
        1 gcgctgcttc tgccatcgcc cgccaataat atgactcttc caaataaggt tcattggtac
       61 tggctaaagt gatatttgcc aaattgatca catcctgata acgtcccgaa taataatagg
      121 cataatatgg accggtctga taccagacga tacgatacgg ccttgttttt gtatccaaat
      181 ttgcatagta tgaaaatgcc atgtcataag attgtgccgc tccgtaataa tccgttaagg
      241 cgacttgtga agtaccgcga ttaaaataag cataaaatcg cgattcatca tcctgtgcct
      301 ccgctacttg attgtttgcc atttcaaaag cattctgttt cgcccaatca ctgttgacat
      361 acggtccgag caaactatag agttcggctt ctttatctcc gggatagacg atcataaatt
      421 caaagttaaa acatttccat tctttataga gcgtttcata agtaacttca taattcggtt
      481 tgtaatatga atcctgaacc gtccaaatct gtgtagcatc attataacca gtaataacgt
      541 tatagtgtcc catccaactg taggcaccgc tgacttcctg catgaaagac cctttttcga
      601 tcaaaatcgg aaagccggct gcaatcaatt ttttcaaaag ttccggtgtt cctcccgatc
      661 tgacaatcga tcgcaactgt gtttgttctg cgacaaagtt ttccatttca taaaacatga
      721 catttttatc tttgtcaaaa ggtttcaacc actttcccgc atccaatcga tttcctttcc
      781 acccccaaaa tgtgagcgcc attgataaat tggtgggggc gcagtaattc caaatcccat
      841 gttggtcttc gtacttgaca ccgtcgatgt gagcactttc gggcaagggg gtcggagtga
      901 tcgttgcggt cggattcaga gtcggcgttg ccgtcgcagc cggcgtgaca ctgacagccg
      961 tttcggtggg agggacggtc attgtcattg ttattgtcgg ggtgtgagtt ccttcggcag
     1021 tctgagataa catcgaaatg ttttcttcgc ctccctgagg gacaaacacg gtatcggcag
     1081 gtgaattgga aagcatatag agtctgttct tcaggttttc gactcgaaaa ctgatcttgt
     1141 cgcgaatagg agtaaataac ccgataaaaa gtatgaaaaa taaaatgatt ccaaacagaa
     1201 taatagggcg ataatccctg tcatttttca taaaaatatt ctatcaaaga gaaattttat
     1261 atggatattg cattcaaaaa gggaaattcc gaatggagcg aatgattcca aaataattaa
     1321 tcttcatctt catccaaatg aacttccaat ttaatttgtt cgggcttaat ccttaaccaa
     1381 aggacaagtt tttgcccttc ttccgtatcc agacagcggg cggctacaat cggcacttcc
     1441 tgttcgagcc cgattgagtt tcgaaattgc aaatacatgg tatcctgttg ctcccatgtc
     1501 gattgacagc gttcaatcaa tccgggaccg ttcaaggtgc gtaaatttgt catcaccact
     1561 tcataattgg ccggagattc aacgatcgca gccgtccaat cggaattggt cgcttcaaaa
     1621 atcggagtgg gtccttctat aatcgtaatc ttgttattca taataaaacc ttctgtttat
     1681 cataccataa tgtcatcttt tatttcttaa aaccttatta aatggttttt taatagtcaa
     1741 gactaaaagg aagaacctcc attagagccg aagttgttat cctgatgatt ttccacaaaa
     1801 cttgattgct catccaatag tggcgtcgat attaggacat ttgtaaatag ccttgacaca
     1861 ccattatggt tgaataacaa cacaaatttt tgtttttctt gcgaaatttt atcagaaaga
     1921 gtatactcgt attgcttttt cggggtgtag cgcagttggt agcgcaccgc gtttgggacg
     1981 cgggggtccg cggttcaagt ccgcgcaccc cgacagatga cccttctttc ccctgactgt
     2041 aaaaccaaac gactcttcga ttttatgttt tttatgataa gatttagggg aaaagccaat
     2101 ggaaagtacg ataaacccct cctctcatat ttatttatcg attattcttc cggcccataa
     2161 tgaagaaaaa cgcttaccca tttgtcttga aaaggtaatc gcatttgttt cttctctccg
     2221 gttccccttt gaagtgattg tggtagaaaa cggcagtgtc gatcaaacat tggctatcgc
     2281 aaccgaatat gcaaaacgat atgactggtt gagagttttg caaaattcaa aacccggcaa
     2341 agggttggcg gttcgaacgg gtatgttggc tgcaaagggg gaatatcgtt tttttgccga
     2401 tgttgatttc tccatgccca ttgaagaaat attaaatttc cttcctccca agctttccga
     2461 ttatgatgtc gctatcggat ccagagaagt taaaggggca attcgctata atgaaccacc
     2521 gatccgtcac ctgaccggtc gagtatttaa tcagattgtc cgtctaatga ccgtacataa
     2581 catccaggat actcagtgcg gtttcaagtg ttttaaagca aacgcggcag aaaaaatttt
     2641 ctcggttcag gttttagacg gttgggcatt tgatgcggaa gtgttgtata tagcccaaaa
     2701 aaacggattt aaaattgtcg aagttccggt acgttggtat tatgacggac aaagcaaaat
     2761 tcatgtgctg aatgattcag tcaagatgtt tcaggaacta ttacaaatac gcc
//
LOCUS       DAOMIV010000267         2813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_38669_length_2813_cov_3.393038, whole genome shotgun sequence.
ACCESSION   DAOMIV010000267 DAOMIV010000000
VERSION     DAOMIV010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38669_length_2813_cov_3.393038"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKV59_06685"
     CDS             <1..735
                     /locus_tag="PKV59_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46476.1"
                     /translation="ILVCIIAGFLGGALFGAIPGYLKAKLGTSEIIVTIMLNYIAIDL
                     LSYLLDGPMREGGSFYPQSALLDQSVWLPKIIAGTRLHAGFIIAIVFDIIYFFLMFRT
                     PLGFKIRAVGFNSKAAEYAGVNVGRSILTAMMLSGGLAGIAGAIEIFGVHHRLLNGFT
                     AGVGFDALAVALLGKLNPVGVFLASLFFAALKVGSNTMQHVMQVPTSIVYVIQGLLIL
                     FVFTDTLIKDKLLYFEKRLKTKQSGE"
     gene            743..1672
                     /locus_tag="PKV59_06690"
     CDS             743..1672
                     /locus_tag="PKV59_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46477.1"
                     /translation="MESNYGITVQGLLAAAVRMATPLLLAGLGGLFSAKSGIVSMFLE
                     GFMLVGAFMGFLGSVLTGSAYLGILFAIAGGMLMGLIMAVLAVSGRANQTILGVGINI
                     FALGITSYFQSVLFGSKRPTNVSVFYDIPIPGLSKIPYIGAIFNNSLFVYAAFLLVPV
                     VWYVLYRTPAGLAIRAVGENPDSVATLGGNVIKIRYCCLLIAGALAGLGGASLTIGQT
                     GQFMENISSGRGYIALAAMIFGRFSPIGVLIGSLLFGFADGLQLRMQTAGTAIPYQLL
                     SMMPYVVTILALVVFVKKSMAPAAMGRPYTKQK"
     gene            1788..>2813
                     /locus_tag="PKV59_06695"
     CDS             1788..>2813
                     /locus_tag="PKV59_06695"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQP46478.1"
                     /translation="MKCDLYIKDCMLLTKDYEIIDHQSIVINNSFIIEMGPTSTLDTK
                     YEPQQTITATDKLAMPGFIDGHTHTSQQLLRGRISDEYPVIYRRFNIPYESKLTEDDI
                     ELCTELSCLEMIKSGITAFADAGSMHIPAMINVVDRSGLRASITPAISDNDPTLPPNM
                     SDTKENALKKSENLFLEYNGKCDGRIHIWFQFRSITTCTSELIRELAKLAAYYHTGLH
                     THISEYPESILQSLNSYGMREIKYLESLGALGPNLLAAHCILLSEEDIHILKNHDVKI
                     VHCPRSNLGKGITKTPQLLAEGLSVGLGTDGTAHSGLNIFKEITAFKHSQIAAWGVPY
                     SDYDVMNS"
BASE COUNT          791 a          525 c          613 g          884 t
ORIGIN      
        1 attcttgttt gtatcattgc gggattttta ggtggagctt tattcggggc gatcccaggg
       61 tatctaaaag cgaaattagg tacatccgag attatcgtta ctataatgct taattatatt
      121 gcgatcgatc tattaagtta tttgctggat ggcccgatgc gagaaggagg aagtttctat
      181 ccccaaagtg cgttattgga tcaatctgta tggcttccaa aaataattgc ggggacaaga
      241 cttcacgccg gttttattat tgcaatagtt ttcgatatta tctacttttt cttgatgttt
      301 cgtactccac tcggttttaa aatacgtgcg gttggtttca attctaaagc ggcagaatat
      361 gcgggagtta atgtaggaag gagtatatta accgccatga tgctttcagg gggtcttgcg
      421 ggaatcgcag gtgcgataga aatattcggg gtccatcatc gattgctgaa tggttttact
      481 gccggtgtcg gatttgatgc attggcagta gctctcttag gaaaattaaa ccccgttggt
      541 gttttccttg cctcgctctt ttttgctgcg ctaaaagttg ggtccaatac aatgcaacat
      601 gtgatgcagg taccgacttc gatcgtttat gtcatacaag gtcttttaat actgtttgtt
      661 ttcaccgata ccttaatcaa agataaactg ctttatttcg aaaaaagatt aaaaacaaaa
      721 caatccgggg agtaatcaaa tcatggaatc aaattatgga attaccgttc aaggtctttt
      781 agctgccgca gttcgaatgg ctacgccttt gcttttagcc ggattgggag gtttgttttc
      841 agcaaaatcc ggtattgtga gtatgttcct tgaaggattt atgctggttg gagcgtttat
      901 gggattctta ggctccgttt tgacaggaag cgcttacctc ggaattttat tcgcaattgc
      961 cggaggaatg ctgatgggtt tgataatggc tgtcttggct gtttccggca gagccaatca
     1021 aacgattctc ggagtgggaa ttaacatttt cgctttgggc atcaccagtt atttccaatc
     1081 ggtcttattc gggtccaaac gcccaacaaa cgtttcggtt ttttatgata ttccgattcc
     1141 tggtttgtca aaaatcccct acatcggtgc gattttcaat aactctttat ttgtttacgc
     1201 agcctttttg ctcgttccgg ttgtttggta tgttttatat cgtaccccgg caggtctcgc
     1261 aattagagct gtaggcgaaa atcccgattc ggtcgccaca ttgggcggaa atgtaataaa
     1321 aatccgatat tgctgtcttt taatcgcagg agccctggcc gggttaggcg gtgcctcatt
     1381 gacaatcggg cagacaggtc aatttatgga aaatatttca tcaggaagag gttatattgc
     1441 tttggcagct atgatcttcg gcagattttc tcccatcgga gtgttaatcg gttcgctttt
     1501 attcggtttt gccgacggtt tgcaattaag aatgcaaact gccgggacag ctattcctta
     1561 tcaattgctt tctatgatgc cttatgtcgt aacgatctta gcgcttgtgg tatttgtcaa
     1621 gaaatcgatg gcaccagcgg caatgggacg tccttatacc aaacaaaaat aatgttgatt
     1681 gttattttga ataaaatatt tatctttttt cttttatcgt gatgtcaaag taagatcgga
     1741 ttcgaataaa caaaacccgt attgcggtaa cccggaagga aaaatttatg aagtgtgatt
     1801 tatatatcaa agattgtatg ttgctgacaa aagattacga gatcatagat catcaatcta
     1861 tcgttattaa taattcattc attatcgaaa tgggacccac atccacattg gatacaaaat
     1921 acgagccgca acaaaccata acagcgactg ataaattagc aatgcccgga tttattgacg
     1981 gtcatacaca tacttcccag caattactaa gaggtcgaat ttctgatgaa tatccggtaa
     2041 tttatcgcag atttaatatt ccatatgaaa gtaagctgac cgaagatgat attgaattat
     2101 gtacggaatt gagttgtttg gaaatgatta aatccggcat tactgcattt gcggatgcgg
     2161 gaagcatgca tattcccgca atgatcaacg ttgtcgatcg atcggggtta cgagcttcca
     2221 tcacgcctgc catttctgat aacgatccaa cactgccgcc caatatgtct gatacaaaag
     2281 aaaatgcatt gaaaaaaagc gaaaatttgt ttttggaata taacggaaag tgtgatggca
     2341 gaattcatat ttggttccag tttcgatcga tcacaacttg tacaagtgaa ttaattcgag
     2401 aattagctaa attagcggca tattatcata caggattaca tacccatatc agtgaatatc
     2461 ccgaatcgat tctacaatct ttaaacagtt acggaatgcg ggaaatcaaa tatctggaaa
     2521 gtctcggagc ccttggtccg aatttattgg ctgcgcattg catattatta tctgaagaag
     2581 atatccatat attgaaaaat catgatgtta aaattgtcca ttgcccgaga agtaatttgg
     2641 gaaaaggcat aacgaaaacc ccacaattat tagctgaagg attatcagta gggctcggaa
     2701 ccgacggtac agcgcattcc ggtttgaaca tattcaaaga aatcacagct tttaaacatt
     2761 cacaaatcgc tgcctggggg gtgccttact ccgattacga tgtaatgaac tcg
//
LOCUS       DAOMIV010000268         2804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_38845_length_2804_cov_3.812659, whole genome shotgun sequence.
ACCESSION   DAOMIV010000268 DAOMIV010000000
VERSION     DAOMIV010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38845_length_2804_cov_3.812659"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PKV59_06700"
     CDS             complement(<1..836)
                     /locus_tag="PKV59_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQP46479.1"
                     /translation="MAQYSQGGDSIFNNAEANWWDNAQNLFFINPELLDDNKPNLSQK
                     KSSGKRSHIISKDRRGNYYKARIAEDAVNDLALDATLRAAAPYQKSRRTNPSDRSILI
                     KHQDLRRKVRSQRASGLLLFLLDLSWSMAVRQRMNATRGAILSLLTDAYQHRDRVGLI
                     TFQKDSAELVLPPTYSVTLAERSMKKVQVGGKTPLTAGLWKAYEVLERERKRHPDENA
                     LLIVLTDGVGNISMFDKAPEEESKIIAEKIAAENFRSIVIDLDTQDFDQGMTRKLAAQ
                     LKA"
     gene            complement(872..1966)
                     /locus_tag="PKV59_06705"
     CDS             complement(872..1966)
                     /locus_tag="PKV59_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP46480.1"
                     /translation="MTANIYPFTAIVGQEQMVRALILNAIDPRIGGVLIRGERGTAKS
                     TAARGLAAVLPEEEVIAECRFGCDPDDELHWCTECKERKAKGIPMTRTRRKTPFINLP
                     VSATEDRVVGTLDIEKALKTGEKSFEPGILASANRGILYIDEVNLLEDHVVDILLDSA
                     AMGVNSIEREGISFSHPARFILVGTMNPEEGDLRPQLLDRFALSVDIRGIEDARERVE
                     IMRRTLRFEDSPELFQAEWQKSEAELTEKIASARKRLASVRYTSRDLITIASLTSSLK
                     VEGHRADLVILKAARAQAAFENHPSVTDLDIAMAAELALPHRLQKGPFSESTVGTDEL
                     KQRIDELHGKTSDSTSFQSESDAGDEEKKI"
     gene            2120..>2804
                     /locus_tag="PKV59_06710"
     CDS             2120..>2804
                     /locus_tag="PKV59_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HQP46481.1"
                     /translation="MESKDGSNGVVQKKMGQQNKRKNSISDLWLKIIQAGLGERLLKI
                     GSAVVTILLIGTVLWVTGVFFMEDSQKDAVNLQKTLRATPVHQSAVLPYYDPAYPKGE
                     LKPLNEGVFRSTKNDTELPAKARTNVIKYIVQKGDTVTSIAEKFNLRPSTILWANPYT
                     LADNPHMVVEGMELNILPVDGVYYDWHDGDGLNGVAEVYGVTPEDIINYPGNGLDPAT
                     IGDYSHPNIA"
BASE COUNT          732 a          627 c          665 g          780 t
ORIGIN      
        1 gctttgagct gagcggcgag ttttctggtc attccttgat caaagtcttg cgtatccaaa
       61 tcaataacga tgctgcggaa gttttctgcg gcgatttttt cggcaatgat tttagattct
      121 tcttccggag ccttatcaaa catcgaaatg tttccgacac cgtcggtcaa aacaatcaac
      181 agtgcattct catcgggatg gcgttttctt tcccgttcca agacttcgta agctttccat
      241 aacccggcgg tcaacggtgt tttcccgccc acctgaacct ttttcatcga tctttccgcc
      301 aaggtaacgg aataggtcgg cggaagcacc aattcggcgg agtctttttg aaaagtgatc
      361 aaaccgaccc gatcccggtg ttgatatgca tccgtcaaaa gagaaaggat tgctccccgg
      421 gtggcattca tccgttgccg gactgccatg gaccaagata aatccagcag aaaaagcagt
      481 aagccggacg cccgttggga acggactttt cggcggagat cctgatgttt aatcaggatc
      541 gaacggtcgg aaggattggt cctgcgcgat ttttgataag gagccgctgc acgtaacgtt
      601 gcatccaacg ccagatcatt taccgcatcc tcggcaatcc gagctttata ataatttccg
      661 cggcgatctt tggaaataat atgacttcgt tttccgctgc tctttttttg cgaaagattg
      721 ggtttgttat cgtcgagtaa ttccggattg ataaagaaga gattttgcgc gttgtcccac
      781 caattggctt ccgcgttgtt aaaaatcgaa tccccccctt gtgaatattg tgccacctga
      841 atagggatta aattttgcag atcggaggaa gttaaatttt tttttcctcg tcacccgcat
      901 cgctctcgga ttggaatgac gtactgtcgg aggttttacc gtgtaattcg tcgattcgct
      961 gttttaattc atcggtaccg acggtgcttt cactgaacgg acctttttgc aggcggtgag
     1021 gtaaagccaa ttccgcagcc atcgcaatat ccagatctgt aacggaagga tggttttcaa
     1081 aagcagcctg tgctcgggcg gctttcaaaa tcaccaaatc cgcacgatgt ccttccactt
     1141 ttaatgaaga ggtaagcgaa gcgattgtga tcagatcgcg agaagtgtaa cggaccgaag
     1201 ccaaacgttt tcgagcggat gcgatttttt ctgtcagttc cgcttcactt ttttgccatt
     1261 ccgcctgaaa aagttcgggc gaatcttcga atcgtaatgt tctgcgcatg atttcgacac
     1321 gctcccgagc atcttcaatg ccgcggatat caacggaaag tgcgaaacga tctaaaagtt
     1381 gcggacgcaa atccccttct tcgggattca tggttccgac aaggataaat cgagcgggat
     1441 gcgaaaaaga gataccttcc cgttcgatgc tgtttacacc catcgcggcg gaatcgagca
     1501 ggatgtccac gacatgatct tcgaggagat tgacttcatc gatatacaaa atcccgcgat
     1561 tggcggaagc caatataccg ggttcgaaac tcttttcgcc ggtttttaat gctttttcga
     1621 tatccaacgt gccgactacg cggtcttcgg ttgcggacac cggtaaatta atgaatggtg
     1681 tcttccggcg agttcgtgtc atcggaatcc cttttgcttt tcgttctttg cattccgtgc
     1741 accaatgtaa ctcatcatcc ggatcacagc cgaaacgaca ttcggcgatc acttcttctt
     1801 ccggtaaaac ggcggccaag cctctggcgg cggtggattt ggctgtgccg cgttcaccgc
     1861 gaattaaaac gccgccgatt cgcggatcaa tggcattcag tatcagggca cgaaccattt
     1921 gttcctgccc gacaattgct gtaaaaggat aaatatttgc tgtcatagga cttttattat
     1981 agcgtcgaac agatgattta ttcattttcg aatcaaaaat taagattctt gtaatctttg
     2041 cggcttcgca aacatttttt aagaaattat tattttatcc gaaacagaaa tatgttatga
     2101 tctaaaaatt gaataaaata tggaatcaaa agacggatca aatggggttg tccaaaagaa
     2161 aatggggcaa caaaataaac gcaagaattc aatttccgat ttatggctga aaattattca
     2221 agccgggttg ggagaaaggc ttctcaaaat aggctctgcg gtggttacga ttttgttaat
     2281 cgggacggta ctgtgggtaa ctggcgtctt ttttatggag gattcgcaaa aggatgcggt
     2341 caatcttcag aaaacactcc gagctactcc ggtccaccaa agcgcggttt taccttatta
     2401 tgatccggct tatccgaaag gtgaattaaa gcctttaaat gaaggtgttt ttcggagcac
     2461 caaaaatgac actgaattac ctgcaaaagc gcggacgaat gtcattaaat atattgtgca
     2521 aaaaggggat actgttacgt cgatcgccga aaaattcaat ttacgtccca gcacaatttt
     2581 atgggcgaat ccttatacac tggctgacaa cccgcatatg gttgtagagg ggatggaatt
     2641 aaatattctg ccggtagacg gggtttatta tgattggcat gacggtgacg ggttgaatgg
     2701 cgtcgccgaa gtttacggag tgacaccgga agatattatt aactacccag ggaacgggtt
     2761 ggatccggca actatcgggg attacagtca tcccaatatc gcac
//
LOCUS       DAOMIV010000269         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_38940_length_2799_cov_4.227041, whole genome shotgun sequence.
ACCESSION   DAOMIV010000269 DAOMIV010000000
VERSION     DAOMIV010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38940_length_2799_cov_4.227041"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PKV59_06715"
     CDS             <1..773
                     /locus_tag="PKV59_06715"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQP46482.1"
                     /translation="IDNYPGVTDNPSGTELMSRFISQAEKFGAVLDYDTAIKVNLSER
                     PFEIETEGKIYLAENLIITTGSRSVTMSVSGEKELTGSGVSYCATCDGFFFKDKKVAV
                     VGGNNEAVEEAVFLTKFASSVTIIHRQGELRADPIRLEKAKENPKIQYLMNSTVTAVL
                     GKDHVEGLRVKNMLTGEELDMPFDGVFVFIGQKPISELYEGQLEMEKGLIKVDDRMRT
                     KIPGVYAAGESVDGFYKQVVVSAGMGAMAGISLIKDLA"
     gene            805..1914
                     /locus_tag="PKV59_06720"
     CDS             805..1914
                     /locus_tag="PKV59_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQP46483.1"
                     /translation="MNQQAEAFIQQKRLVETFLALVRVDSPSGHEAKLSDALVKELIK
                     LDGDPHQDEIGNIVAYFKGNGSDDTVMLCAHMDTVGNDVGIQPIVLSDRITSDGTTIL
                     GADDKSGIAVILEILKVFKEHPEISHPALEVVLTVQEEVGLVGSSYLDKSQLKAKWGL
                     VLDSGGRIGGITVSGPTSRKYTFTILGKAAHAAVAPEQGINAIQAAALGITKCPCGKV
                     DPEVVIGIGVIQGGIATNIVPDKVEVKAMIRSRNPEKLERFSEQMENSFREGCEPFGA
                     KSEAEKSDSYQGYLLDENSVPWQKTKKAAELLGFPFFTEHSLGGTDCNHFNQAGIPCC
                     SVSTGMANEHMKDEYIRIQDLYDAARHVITAITIA"
     gene            2525..2698
                     /locus_tag="PKV59_06725"
     CDS             2525..2698
                     /locus_tag="PKV59_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQP46484.1"
                     /translation="MKRKDLIKLLEKNGWELVRNGAQHDVYRKGKDQETIPRHSEIPE
                     ILAKKIIKRRNLK"
BASE COUNT          848 a          517 c          701 g          733 t
ORIGIN      
        1 aaattgacaa ctaccccggc gtaaccgata accccagcgg caccgaatta atgagccgat
       61 ttatctcaca agctgaaaaa ttcggcgctg ttttggatta tgacacagcc attaaagtca
      121 atttatctga acgtccgttt gaaatcgaga cggagggaaa aatatatctg gcggaaaatc
      181 tgatcatcac taccggttcc agatcggtta cgatgtctgt ctctggtgaa aaagaattaa
      241 ccggatctgg agtttcctat tgcgcaactt gcgatggctt tttctttaag gataaaaaag
      301 tggcggtggt cggtggaaat aacgaagcgg tggaagaggc ggtttttctg acgaaatttg
      361 cttcatcggt aacgatcatt catcgtcagg gtgagctgcg ggctgatccg attcgactcg
      421 aaaaagcaaa ggaaaacccg aagattcaat atctgatgaa ctcaaccgtc accgctgtgt
      481 tggggaaaga tcatgtcgaa ggcttgagag tcaaaaacat gctgaccggc gaagaactgg
      541 atatgccatt tgacggtgtc tttgtattta tcggtcaaaa accgatctcg gaattatacg
      601 aaggtcagtt ggagatggaa aaagggttga tcaaagtgga tgaccggatg cggacaaaaa
      661 ttccgggtgt ttatgcggcc ggtgaaagcg tcgacggctt ttataaacag gtggtcgtgt
      721 ccgccggaat gggtgcgatg gcgggtattt cattgattaa agatcttgcc tgatcagcaa
      781 cggaagacgg aaaggaacca gatcatgaat caacaagcgg aagcttttat tcagcagaag
      841 cgattggttg aaactttttt agcgcttgta cgggtggaca gcccctccgg tcatgaagcg
      901 aaattgagtg acgcgttggt gaaagaattg atcaaactgg atggagaccc gcatcaggat
      961 gaaatcggca atatcgtcgc ctatttcaaa gggaacggca gcgatgatac cgttatgctt
     1021 tgcgcccata tggacacagt cggaaacgac gtgggaattc agcccatcgt attatcggat
     1081 cggatcacat ccgacgggac gaccatttta ggtgccgacg ataaatccgg aattgccgtc
     1141 attctggaaa ttctgaaggt ttttaaagag catccggaaa tcagccaccc cgcgctggaa
     1201 gtggtactga ccgttcagga agaagtcggg ttggttggat cgtcctatct ggacaaatcg
     1261 caactgaaag cgaaatgggg actggttttg gattccggtg gaagaatcgg gggaatcacc
     1321 gtatccggtc cgacctcgcg aaaatatacg tttacgattc tcggcaaagc cgctcatgcc
     1381 gccgtggcgc cggaacaagg tattaatgcc attcaggcag ccgcgttggg gattaccaaa
     1441 tgcccgtgcg gaaaggtcga ccccgaagtc gtgatcggga tcggcgtcat tcaagggggc
     1501 atcgccacca atatcgttcc ggataaagtc gaagtaaaag cgatgattcg cagccggaat
     1561 ccggaaaaac tggaacgatt cagcgaacag atggaaaatt cttttcggga gggatgtgag
     1621 cccttcggag ccaaatcgga agcggaaaag agcgattctt atcaggggta tctgctggat
     1681 gaaaattccg tcccttggca aaaaaccaaa aaggctgcgg aattattggg ttttccgttt
     1741 tttaccgaac attcgttggg cggaacggat tgcaatcatt tcaatcaagc cggtattccg
     1801 tgttgttccg tttccaccgg catggccaat gagcatatga aggatgaata tatccgtatt
     1861 caggatttat atgacgcagc ccggcatgtg attacggcga taacgatcgc ataaaaaggg
     1921 ttcgttcggc attttatcgg tccggcatcg cttttgagtt gtgccggacc ttttctttac
     1981 cggtgatgat catatgggcc tgcttttcgg tgtcacctcc gtcaagtaat ttgaattttt
     2041 cgtgtttgca aagaatattt ccagagatcg atactgtttt gctctctatg aatatatttc
     2101 aatagttttc tgctaattgt ctcttttaaa aaaatttaac agcgtagatt tgttaaaaag
     2161 tgtctgttat ctattagtaa ctatcatttt agaaatattt tttaaaagat cgacggttta
     2221 aaaatcagtg aaacatgaaa aaagaggtat acgaattacg tcgaaaagtt aagtaagcgg
     2281 agaatctgtt taaattgttt attcaatagg cttacagaat ataagcaagg tattttcatc
     2341 tattcaaaaa cgaagaaatg ccgataaatg atgaatcggt agagaaaaaa tgtactcgaa
     2401 ataattctta ttaataaata ttggcgatat gagtcggcag attctctcat taaataggtt
     2461 ttattattac agaataactt gacaatacgt ataaaatact tataatattt tcaaaggaaa
     2521 ataaatgaaa cgaaaagacc taattaaact gttggaaaag aatggatggg agttggttag
     2581 aaacggtgca caacatgatg tatatcggaa agggaaagac caagaaacaa ttccgagaca
     2641 ttcagagatt ccggaaattt tggcaaaaaa aatcattaaa agacgaaatt tgaaataacc
     2701 ggaacagtcg gagtttgatt aggcaatgga ggaggcaatt atgaaagcgg tttatccgat
     2761 cgtcctgacg aaggatggtc aatattatat tgtggatgt
//
LOCUS       DAOMIV010000270         2795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_39043_length_2795_cov_3.910219, whole genome shotgun sequence.
ACCESSION   DAOMIV010000270 DAOMIV010000000
VERSION     DAOMIV010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39043_length_2795_cov_3.910219"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1003)
                     /gene="jag"
                     /locus_tag="PKV59_06730"
     CDS             complement(188..1003)
                     /gene="jag"
                     /locus_tag="PKV59_06730"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HQP46485.1"
                     /translation="MEFSAGTVDEAIEKGLKALGKTRDEVDIVVIDSGAKGIFGIGSR
                     PASVSLKVKESAAKAPQSESSQTGRTVKPESDKISAKEISEELPGDVNKADTTEAESE
                     NEEEKTAGEELVDETALKATVTVVEDLIDKMHIKASVTGRIGKLETSGNQEMILVDIK
                     GDDLSYLIGRHSETLNAFQYITSLIVGRELGHWVPMMIDVQGYRERRERQLRQMAIHM
                     ADQVTRSGRRISLEPMPGTERRIIHLALRDRNDIYTESVGEEPNRKVVIYPKN"
     gene            complement(1044..1985)
                     /locus_tag="PKV59_06735"
     CDS             complement(1044..1985)
                     /locus_tag="PKV59_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HQP46486.1"
                     /translation="MWQSFVNIFLNILLYIYKLVGNFGVAIILFTVLIKLITYPLMRK
                     QIQSSTKMMELQKSPEYLEMMEKYKGNQEKLAEEQMKLYKKFGVNPTASCLPTLIQLP
                     IIFALYQAIIAAIVSTPLGMLDLTKRAFSGFLQIGEIFPINPNFLWMNLGQPERLYIQ
                     GISFGIPVLAILVVITTIMQTKLTVPPTNPNDKNAASGAMMTGMMNIYMPLLMGFMAY
                     TLASGLSLYFLISNIFTIVQYGVEGKLNWKALKFGKPQMPKLATPAEKPVTTSRPVAS
                     ANPKKSMTPNLAESGIEPAEEKIDYREFKNKKKPKKK"
     gene            complement(1998..>2795)
                     /locus_tag="PKV59_06740"
     CDS             complement(1998..>2795)
                     /locus_tag="PKV59_06740"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQP46487.1"
                     /translation="ASPVIADDMLFIASNNGSVYAYTLDNLSVPVWQTNLSTEIWVSP
                     FVSDGKVYVTTLDKRFLVLDEKTGTIEKALSISGAVMGKLVFANDRIYFGTLGKEVAY
                     FDPATEEIKTVMHTNGEIWASPLIIDGKIIVADMSGYLYCNESESGKSIWQIKAVSTD
                     KVGFIADPILLQNGNLLAVAEDGNLMVYDTDGKSVNSRLINGKTMSSPVVIGESFVAG
                     LIPGDALLRSYTSDLKEDWWYIETVESTALTPTAEVSQETVTETPAK"
BASE COUNT          678 a          671 c          589 g          857 t
ORIGIN      
        1 atgaaaaatg ccgattattt atgcttcagt tataaaaata ctccgggaaa cgattatcaa
       61 atcccggatt ttgccgtgat gaaaaccttt atggtggacg gatatgcggt aatgggagtt
      121 tatcaacggg taaaccccgc cgaataagaa agagggggct ttttcaaaag ccccctcttt
      181 ctcccgttta atttttcgga taaatcacta cttttcgatt aggttcttcc ccgacacttt
      241 cggtataaat atcatttcga tcccgcaacg cgagatgaat aattcttctt tccgttccgg
      301 gcatcggttc gagagaaatt cttcggccgg agcgagtaac ttgatccgcc atgtgaatag
      361 ccatttgtcg tagttgacgt tcccgtcgtt ctcggtatcc ttgcacgtcg atcatcatcg
      421 gtacccaatg gcccaattca cgtccgacaa ttaaacttgt tatatattgg aatgcattca
      481 atgtctcgga atggcgaccg atcagataag ataaatcatc ccctttgata tcgacaagaa
      541 tcatttcctg attgccggat gtttcaagtt ttccgatccg tccggtcaca cttgccttga
      601 tatgcatttt gtctattaag tcttcgacga ccgtcacagt ggctttcaat gcggtttcat
      661 caaccaactc ttcgccggcg gtcttctctt cctcattttc agattccgct tcggttgtat
      721 ccgctttatt cacatcgccg ggaagctctt cgctgatttc ttttgccgat attttatcgg
      781 attccggttt gacggttcgg ccggtttgag aagattccga ttgcggagct ttggctgccg
      841 attccttcac cttcaagctt acggaagccg gccttgagcc gattccaaag atcccttttg
      901 ctcccgaatc gatcacaaca atatcgacct catcccgtgt ttttcccaga gcttttagcc
      961 ctttttcaat tgcttcatcc acagtccctg ctgaaaattc catttgattg tccatagtct
     1021 atattgcctt atctactctg tcgtcacttt ttcttcggtt ttttcttgtt tttgaactca
     1081 cgataatcaa tcttctcttc cgcgggttct atccccgatt ccgctaaatt gggcgtcatc
     1141 gattttttgg gattggcact ggcaacggga cgggaagttg ttaccggttt ttcggccggt
     1201 gtagctaatt tcggcatttg cggtttaccg aactttaagg ctttccaatt taattttcct
     1261 tccacaccat attgtacgat cgtaaaaatg tttgaaatca agaaatagag cgaaagaccg
     1321 gaggctaatg tgtaagccat gaaacccatc agaagcggca tataaatatt catcataccg
     1381 gtcatcatgg ctccgctggc tgcgttcttg tcattcggat tggtcggcgg gacggtcagt
     1441 ttggtttgca taatggtcgt gattacaacc agaatcgcca aaaccggtat gccgaaagag
     1501 atcccctgaa tgtacaatct ttcaggttgc cccaaattca tccacaagaa attcggattg
     1561 atcgggaata tttcaccgat ttgtaaaaag ccggaaaaag cgcgttttgt caaatccaac
     1621 attcccaacg gagtcgatac gattgccgcg ataatggctt gatataatgc gaaaataatc
     1681 ggaagctgaa tcagtgtcgg caggcaggaa gcggtaggat tcactccgaa ctttttataa
     1741 agtttcattt gctcttctgc cagtttctcc tgatttcctt tgtatttctc catcatttcg
     1801 aggtattcgg gagatttttg cagctccatc attttggttg agctttggat ctgctttcgc
     1861 atcaacggat aggtgatcag tttaatcagg accgtaaata agatgatcgc gaccccaaaa
     1921 ttcccgacca gtttatagat atagagcaaa atattcagaa agatatttac aaaagattgc
     1981 cacatagtca ttccctccta ttttgccggt gtttctgtga cggtttcttg gctgacttcc
     2041 gcggtcggtg taagtgcggt cgattcgact gtttcgatat accaccaatc ttctttcaga
     2101 tccgatgtgt aacttctcaa gagtgcatct ccggggatca aaccggcgac aaatgattca
     2161 ccgataacaa ccggcgagct cattgttttc ccattaatca gacgactgtt taccgatttc
     2221 ccatccgtat cataaaccat caggttgccg tcttccgcga ccgccagaag attcccgttt
     2281 tggaggagga tcggatctgc gataaatccc actttgtcgg tggaaacggc tttaatctgc
     2341 catatagatt tcccggattc cgactcattg caataaaggt atccggacat gtccgcgacg
     2401 ataatcttgc catcgatgat cagcggcgat gcccaaattt ccccgttggt gtgcatcacc
     2461 gttttgattt cttccgtagc cggatcaaaa taagccactt cttttcctaa cgttccgaaa
     2521 tagatccgat cattcgcgaa taccaatttc cccatcacag cgccgcttat ggacagtgct
     2581 ttctcgattg ttccggtttt ttcatccaaa accaggaatc gtttatcgag tgttgtgaca
     2641 tagacctttc cgtctgacac gaatggactg acccatattt cggtggaaag gttcgtttgc
     2701 cagacgggaa cgcttaagtt atccaaagtg taagcgtaga ccgaaccatt atttgaagcg
     2761 atgaaaagca tgtcgtctgc gattaccgga gaggc
//
LOCUS       DAOMIV010000271         2787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_39202_length_2787_cov_3.729502, whole genome shotgun sequence.
ACCESSION   DAOMIV010000271 DAOMIV010000000
VERSION     DAOMIV010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39202_length_2787_cov_3.729502"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PKV59_06745"
     CDS             <1..545
                     /locus_tag="PKV59_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46488.1"
                     /translation="REFLSSGDAEKIKGVAYHNQIDVLSLSTFLIYLSNLMQLNLNDT
                     EINDQCGISVQELRKNLLNHNPIDNPEIFCGNTFPVAVRKKAAAYLQKHRETEKAIEI
                     YRSLVTEGDVDSCLALSSIYSKSTETTPQAIEYLKKGAEFVESDLCMGIWSKKEKQEK
                     IKTQIEKMERKLRKNHGREK"
     gene            529..840
                     /locus_tag="PKV59_06750"
     CDS             529..840
                     /locus_tag="PKV59_06750"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQP46489.1"
                     /translation="MAGKNEIIYWAENEAEAVVIVAILEENGIPAIKLRESYGALNNL
                     SFGIFGEIAIAVDEDRAEEATAIINRQQESAANSDTNQENDENDASSGEIDKDDRDDP
                     F"
     gene            complement(888..1745)
                     /locus_tag="PKV59_06755"
     CDS             complement(888..1745)
                     /locus_tag="PKV59_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQP46490.1"
                     /translation="MDNFWKLHAVNDHYSITSLSEGNFVSINDSSKYTPDGAYTTFRT
                     YNRFFVLNLIKHFDRLEETARLAGKPVLLNRKEICKFLGEVIETYPANELRIRITVDL
                     SVCLGDIYIVLETLITPDIKCYENGIDVITTTLHRSNPKAKLNKFLSQANEVRRQKGF
                     EYEEILMVDSNNEILEGLSSNFYAVIRDEIFTAEEGVLFGTTRDYVLRLAKKLAIPVN
                     LRPVSIHELKYLDEAFITSTSRSILPIRSIDNQKIDEKAPGPITKKLMQAFTEDINSA
                     LEDIRTIGL"
     gene            complement(1786..2373)
                     /locus_tag="PKV59_06760"
     CDS             complement(1786..2373)
                     /locus_tag="PKV59_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQP46491.1"
                     /translation="MKIGSIILAAGNGSRFQAEQHKLLVRLNGKAVVRYSAEKALQAG
                     FLPVIVVLGYQKDDILVQLQGLPVLCIENPDWQSGQSTSLKKGIELIQNEVDAACLML
                     ADQPFVSIRTLRKLEEVQKKNPESIIVPTYQGKNGNPTMFPHWTFQGLIDQPANDLGG
                     RNIIKQYGAKYVEVKDPFVIRDIDTKEDLIDYSKK"
BASE COUNT          836 a          547 c          546 g          858 t
ORIGIN      
        1 atcgtgaatt cctttcttcg ggtgacgccg aaaaaataaa aggagtcgca taccataatc
       61 agatcgatgt cttgagttta agtacctttc tgatttatct gagcaacctg atgcagttga
      121 atttaaatga cactgagatc aacgatcaat gcggaatctc agttcaagaa ctgagaaaaa
      181 atctattgaa tcataatccg attgacaacc cggaaatctt ttgcgggaat acttttcctg
      241 tcgcagtccg aaagaaagca gcggcatatc tgcaaaagca cagagaaaca gaaaaagcga
      301 tcgaaatata tcgatcttta gtaacggaag gagatgttga cagctgcctt gctttatcat
      361 ccatttattc caaatcgacg gaaactaccc cgcaagcaat tgaatatttg aaaaaagggg
      421 cggaatttgt cgaatcagat ctttgcatgg gtatttggtc aaaaaaagaa aaacaagaaa
      481 aaattaaaac tcagatcgaa aaaatggaaa gaaagttgag aaagaatcat ggccgggaaa
      541 aatgaaatca tctattgggc tgaaaatgaa gccgaagcgg tagttatcgt tgcgattttg
      601 gaagaaaacg gaatcccggc aataaaattg cgggaaagtt acggcgctct aaataatctt
      661 tctttcggaa tttttggaga aattgccatc gctgtggacg aagatcgagc ggaggaggca
      721 acagcaatca ttaatagaca gcaagaatcc gcagcaaatt cagacacgaa tcaggagaat
      781 gatgaaaatg atgcatcatc cggtgagatt gataaggacg atcgcgatga tccattttaa
      841 tctggataaa gccgagaaga ggtttctcgg tttctttatg caggacctta taatccgatt
      901 gttcggatat cttccaatgc cgagttgatg tcttcggtaa aagcttgcat taattttttg
      961 gtaatcggac ccggagcttt ttcatcgatt ttttgattat caatgctgcg tatcggaagg
     1021 atagatctgc tggtacttgt gataaatgcc tcatctaaat acttcaattc atggatagaa
     1081 acaggtcgaa gatttaccgg aattgcaagc tttttcgcta aacgcagaac ataatcccgc
     1141 gttgttccga ataatacacc ttcttcggcg gtaaatattt catctcgaat aacggcgtaa
     1201 aaattactgc tcagcccttc caaaatttca ttattactgt ctaccataag aatttcttca
     1261 tattcgaatc ctttttgtct ccgaacctca tttgcctgag aaagaaattt gtttagcttt
     1321 gcttttggat tcgatcgatg taaagttgtc gtaatcacat cgattccgtt ttcataacat
     1381 tttatatccg gtgtaatcaa agtctccaag acgatataga tatctcccaa gcaaacggac
     1441 agatcaacgg ttatgcgaat ccttaattca ttggcgggat atgtttcgat gacttctcca
     1501 aggaacttgc aaatttcttt ccgattcagt aataccggtt tcccggcaag tcttgccgtc
     1561 tcttccagtc tgtcaaagtg ctttatcagg ttcaaaacaa aaaaccggtt gtaagtccga
     1621 aatgttgtat aagccccatc cggcgtatat tttgaggaat catttataga tacaaaattc
     1681 ccctcggaaa gtgaagtgat tgagtaatga tcgtttacag catgtaactt ccaaaaatta
     1741 tccattttgt ttgtccctta tttccgtata ttttttacac ggaatttatt ttttcgaata
     1801 atcgataaga tcttcttttg tgtcaatatc tcggatgaca aaaggatcct tgacctcgac
     1861 atattttgca ccgtattgct tgataatatt gcgtcctccc agatcattcg ccggctgatc
     1921 gattaatcct tgaaaagtcc aatggggaaa catggtcggg tttccgtttt ttccctgata
     1981 ggtgggcaca attatacttt cggggttctt tttttgaacc tcttccaatt tccgtagagt
     2041 tcggattgaa acaaagggtt gatcagccag cattaaacaa gcggcgtcga cctcattttg
     2101 aatgagttca attccttttt tgagagaagt cgattgtccg gattgccaat ccggattctc
     2161 aatgcaaaga acaggtagtc cttgcagttg aaccaaaata tcatcttttt gatatcccaa
     2221 tacaacaatc acaggtaaaa agccggcttg gagggctttt tctgccgaat aacgaacgac
     2281 agcctttccg tttaatcgaa ccaataactt atgttgctcg gcttgaaaac gactgccgtt
     2341 ccctgccgcc aatatgattg atccgatttt cataaacttt ttccctgttt cattatgccc
     2401 ttttgcgctt attattatat ccaatccgtc ggcagaaaca tggtcgatcc ggaaggtatc
     2461 atgaaatggg tagaaaaatc aatctcttca aagaaattcg tccgaatccc ggagaaaaga
     2521 tcctcattta tacggcgggt atatgctata ttttatttat ttcaattgat ttcatagagt
     2581 ttaatccctt taatctttcg gcgaaattaa aatatttatc aatttggatt tgcttcggaa
     2641 ttacactgtt tcgatttttc tcaatcaatc gccccggttt ttctatggaa aagaagatcg
     2701 gctggattaa tataacggca tttttcttta cactcagcgc cgattacttt cttcttttta
     2761 ccgaacaata cggtttggga attacgc
//
LOCUS       DAOMIV010000272         2786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_39234_length_2786_cov_2.916514, whole genome shotgun sequence.
ACCESSION   DAOMIV010000272 DAOMIV010000000
VERSION     DAOMIV010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39234_length_2786_cov_2.916514"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKV59_06765"
     CDS             complement(<1..454)
                     /locus_tag="PKV59_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HQP46492.1"
                     /translation="MDVKKLVSQMTLEEKAGLCSGKTMWHTKSVERLGIPSIMVSDGP
                     HGLRKQDLTKTEHPDHDDSIIAVCFPTAAGLASSFDRDLIYRMGEALGEECQAEDVAI
                     ILGPGANIKRSPLCGRNFEYFSEDPFLSGEMAGAHIRGVQSKNIGTSLK"
     gene            complement(480..>2786)
                     /locus_tag="PKV59_06770"
     CDS             complement(480..>2786)
                     /locus_tag="PKV59_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46493.1"
                     /translation="FYRDNEMNALPLMEYDYSFSRPFEIPDELLNVDRLLLRCEGLDT
                     LGTILINGEKIAETNNMHRIWEFDISESLHSGQNEIEIIFSSPTRFIRKAYEEDPYEG
                     SGDAMRGFVHLRKAEYMFGWDWGPRLPDAGIWRDISLIGIDHARFDNIYVRQEHRDGK
                     VILTFDTELDLVDELPYEIRISVETPSGEILTAADSDRIVIDHPELWWPNELGGQPLY
                     RVSAELLYEGKVLDTWQRRIGLRTLTIRREKDQWGESFCHHINGIDIFAKGADYIPED
                     NILSRITPERTRALLENAVEAHFNTIRVWGGGFYPPESFYETCDELGLLVWQDFMFAC
                     GAFRLDEKSEQNMREEFRDNIIRIRNHPSLALWCGNNEIEEFAAKNQWVKTPKQHSEY
                     ISLFEYIIPHMVRQYDPQTFYWPSSSSSGGGFDDPQDPSRGDVHYWDVWHGEKPFSDY
                     RKYYFRYVSEFGFQAYPPLQTLHTVTLPEDLNAFSRIMEKHQRNASANGKIMKYIQQT
                     FLYPKDFESLVYTSQLLQAEAIRYGVEYWRSIRNCCMGTIIWSLNDCWPVISWASIDY
                     YGRWKALHYAAKRFFAPVLLNALETGENTIYPDINAERIFPVKNSVVFNITNDTWEEV
                     NSEIIWSHRSNDSQIRHQGKITVTVPPFTAKQTDEILFPDFSTYDDYISFSLFVEGKE
                     ISHSSVLLSAPKHFHFLDPHLTVQAEGEELIVRSEAYAKGVFIDNEKGDLKLEDNWFD
                     MDAGEKRIKVLRGTLSNLNVRSVYNIAY"
BASE COUNT          766 a          654 c          591 g          775 t
ORIGIN      
        1 gtttcagtga agtcccgata tttttggatt gaactccgcg aatatgcgct cctgccattt
       61 cgccggaaag aaacggatct tccgaaaaat attcaaaatt acgaccgcat aagggagatc
      121 gtttaatatt ggctcccggt ccgagaataa tcgccacatc ttccgcttgg cattcttctc
      181 ccaatgcttc tcccattcga taaatcaaat cacggtcgaa tgagcttgcc aaaccggctg
      241 ccgtagggaa acaaacagcg atgatactat cgtcatggtc gggatgctcg gtcttggtaa
      301 gatcctgttt tcgcaacccg tgcggaccgt cggaaaccat aatggacgga atgcccagcc
      361 gttcgacaga tttggtatgc cacatggttt ttccggaaca taatcctgct ttttcttcaa
      421 gtgtcatttg tgaaacgagt ttttttacat ccatgaaatc atctcctttt ctgagtgtat
      481 taatatgcaa tgttgtaaac cgagcgaacg tttaaattac tgagcgtccc tctgagaact
      541 ttaattcttt tttctccggc atccatatca aaccagttat cctcgagttt cagatcgcct
      601 ttttcattat cgataaatac cccttttgca taagcttccg agcgcacgat gagttcctca
      661 ccttcggctt gaacggtcaa atggggatcg aggaaatgaa aatgcttggg tgcgctgagc
      721 agaacgcttg aatgtgaaat ttctttcccc tccacgaaaa gcgaaaaact gatgtagtca
      781 tcataagtgg aaaaatccgg gaaaagaatt tcatccgttt gtttggctgt gaaaggcggg
      841 acagtgacag ttattttccc ttggtgacga atctgtgaat cattggatcg gtgagaccag
      901 ataatttcgc tgttcacttc ttcccaagta tcgttggtaa tattaaaaac gaccgaattc
      961 tttaccggaa agatgcgttc ggcattgata tccggataaa ttgtattctc accggtttcc
     1021 aaggcgttta ataaaaccgg agcgaaaaag cgcttagcgg catagtgcag tgctttccac
     1081 cgcccgtaat aatcgattga cgcccaggag atcaccggcc agcaatcgtt taaactccaa
     1141 ataattgttc ccatgcaaca attgcgaatg cttcgccaat attccactcc gtagcgaatc
     1201 gcctcggctt gtaataactg tgaggtgtag accagcgatt cgaagtcttt tggatagaga
     1261 aaagtctgtt gaatatattt catgatcttg ccgtttgcag aggcattccg ttgatgcttt
     1321 tccatgattc tcgaaaaagc atttaaatct tccggcaaag tgacagtatg aagagtttgc
     1381 aaaggcggat aggcttgaaa cccaaattcg gagacataac gaaaataata ctttcgataa
     1441 tcggaaaacg gcttttctcc gtgccagaca tcccagtaat ggacatctcc gcgggacgga
     1501 tcctgcggat catcgaaacc gccgccggag gacgatgagg aaggccagta aaatgtttgc
     1561 ggatcgtatt gcctgaccat atgcggaatg atatattcaa aaagagagat atattcactg
     1621 tgctgtttcg gagttttgac ccattggttt ttagccgcaa attcttctat ttcattattg
     1681 ccgcaccata aagccagaga cggatgattt cggatgcgga tgatattatc gcgaaattct
     1741 tcccgcatat tctgttcgga cttttcatcc aaacggaaag cgccgcaggc aaacatgaaa
     1801 tcctgccaaa ccaaaagtcc cagttcatcg caggtttcat aaaaagattc aggcggataa
     1861 aaccctcctc cccacacccg aatggtattg aaatgggctt ccacggcatt ttccaataaa
     1921 gccctggttc gttccggtgt gattcgactg agaatattat cttcggggat ataatccgca
     1981 ccttttgcga aaatatctat cccgttaata tgatggcaga aactttcacc ccactgatct
     2041 ttttcacgac ggatcgtcag agttcttaaa ccgatccgtc tttgccaggt atccaatact
     2101 tttccttcat ataataattc ggcactaacc cgataaaggg gttgtcctcc caattcattc
     2161 ggccaccaca gttcgggatg atcgataaca atgcggtcgc tatccgcggc tgttaagatc
     2221 tctccggaag gagtctcgac acttattcgg atttcatagg gaagctcatc caccaaatcc
     2281 aattccgtat caaaggttaa gattactttt ccgtcccggt gctcttgacg aacataaata
     2341 ttgtcaaaac gggcatgatc gatcccgatc aacgagatat cgcgccagat tcccgcgtcc
     2401 ggaagtctcg gaccccaatc ccaaccaaac atatattcgg ctttccgtag atgaacgaat
     2461 ccgcgcatgg catcccccga gccttcgtaa ggatcttctt cataagcttt tcgaataaat
     2521 cgggtgggtg aagaaaaaat tatttcaatt tcattttgcc cggaatgaag cgattcggag
     2581 atatcaaatt cccagatgcg atgcatatta tttgtttcgg cgattttctc gccgttgatc
     2641 agaattgttc ctaacgtgtc caatccttcg cagcgcagaa gcaatcgatc gacattcagc
     2701 agttcatcgg gaatttcgaa aggtcgggaa aacgaataat catattccat taaaggcaag
     2761 gcattcatct cattgtcccg ataaaa
//
LOCUS       DAOMIV010000273         2778 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_39397_length_2778_cov_4.446934, whole genome shotgun sequence.
ACCESSION   DAOMIV010000273 DAOMIV010000000
VERSION     DAOMIV010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39397_length_2778_cov_4.446934"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..355)
                     /locus_tag="PKV59_06775"
     tRNA            complement(282..355)
                     /locus_tag="PKV59_06775"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Val,seq:tac)
     gene            complement(418..759)
                     /locus_tag="PKV59_06780"
     CDS             complement(418..759)
                     /locus_tag="PKV59_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HQP46494.1"
                     /translation="MERKIETLNEYQAQCMRTTIGGMDEKMTHLAWSLALAGEAGELA
                     NRTKKVFIHGHPYDQDRVIEELGDILWYVAVYAHDIGVDLEEVAERNIEKVAKRYPNG
                     FSAEASINRKE"
     gene            883..>2778
                     /locus_tag="PKV59_06785"
     CDS             883..>2778
                     /locus_tag="PKV59_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HQP46495.1"
                     /translation="MNFDKYTQKAQEAVAQSQQIAIEFSHQVIEPAHLLLALLRQQDG
                     TVPAIVTRVSGSVAGLLNDVEKSLANQPKVYGSSGNLSMSRTTADVFSNAEKIAKGMQ
                     DEYVSTEHLFLALCDSVEGRRLEQFGLTKDSILKALMSIRGSQRVTSQNPEDTYQSLE
                     KYGRDLTAIARQGKLDPVIGRDDEIRRTIQILSRRTKNNPALIGEPGVGKTAVVEGLA
                     QRIVKGDVPEGLKNKRLVQLDMSALVAGAKYRGEFEERLKAVLKEITDSEGQIILFID
                     ELHTIVGARAAEGSMDAGNMIKPMLARGELHLIGATTLNEYRKYIEKDAALERRFQTV
                     FVDEPSVEDTISILRGLKQRYEVHHGVRITDAAVIAAATLSNRYITDRQLPDKAIDLI
                     DEAAARLRTEIDSKPQILDSVDREIMQLEIEKQALQKEHDQASKDRLAKLEEELANLK
                     EKSNQLTARWHTEKEAIQNLKTVKEKLESLHIQIEQAERANNLELAARLRYGELPALE
                     KQLAAYEEKLKEIQKEGALLKEEVDEEEIAQVVANWTGIPVSRLMESEKQKLVHMEER
                     LHQRVVGQDEAVKAVSNAVRRSRAGLQDPNRPIGSFIFLGPTGVGKTELAKALAEFLF
                     DDERAMIR"
BASE COUNT          853 a          605 c          646 g          674 t
ORIGIN      
        1 catcagtaat ggtatccagc aaagcgcata aaaaaatacc aataaaaaaa atcaaaaaga
       61 aaaggctatc agcccaacat aagtggtggt ggcactgaca aacaaatgta gaaagcaatt
      121 gccttatccc taggcaaaaa atatgaggag tacaaaagta gtggctagga ttccgattgg
      181 gaggaagata aaaaaaaata agtcaatttc cccgtataat aaaggggtgc cacattgagt
      241 caagcacctc tttttttgtt tgttacactg ttgatcggaa gtgggcggaa caggactcga
      301 acctgcgacc ccatccttgt aagggatgtg ctctaaccaa ctgagctatc cgcccgaatg
      361 acgcagcgaa ttataacata aaccgctgga aatattcggt ttccccaatg agaaatttca
      421 ctcttttcga ttgatgctcg cctcagcact gaatccgttc ggatatcgct tggcgacttt
      481 ctcgatattt ctttccgcaa cttcttccaa atcgacaccg atatcatggg catagacggc
      541 cacataccag agaatatccc ccaattcttc aatcactctg tcttggtcgt aaggatgtcc
      601 gtgaataaat acttttttgg tccggttggc taattctccc gcctctcccg ccaatgccag
      661 agaccaggcc aaatgagtca tcttttcgtc catgccgccg attgttgtcc gcatacattg
      721 tgcttgatat tcattcaatg tttcgatttt tctttccatt ttgcctcttt ccgttaataa
      781 ctttcatata tccaccccct ataatagtat cagcgtcttg aaatagattc ttaatattac
      841 taaaaataac ccttttaaaa ggtatataac ggagaagaaa atatgaattt tgataaatat
      901 acacaaaaag ctcaggaggc agttgcccag tctcaacaga ttgcgattga attttcgcat
      961 caagtgattg aaccggctca tctgttactg gcgcttttac ggcaacagga cggtacggtt
     1021 cccgctattg tcacacgggt ttccggcagc gtcgccggac ttctcaatga tgttgaaaaa
     1081 tcactggcaa atcaaccgaa agtttatgga agcagcggta atctttcaat gtcgcgaacg
     1141 acggcggatg tcttctcaaa tgccgaaaaa attgccaagg ggatgcaaga tgaatatgta
     1201 tccacggaac atttattcct ggccctttgt gacagcgtgg aaggaagaag attggaacaa
     1261 ttcggactga cgaaagacag tattctgaaa gcattaatgt cgatccgcgg aagtcagaga
     1321 gtgaccagtc aaaacccgga agatacgtat cagtcactgg aaaaatacgg tcgtgatttg
     1381 accgctatcg cccgtcaagg gaaacttgat ccggtgatcg gtcgggatga tgagatcaga
     1441 cgaacaattc aaatcctttc ccgcaggacg aaaaataacc ccgctttaat cggggaaccc
     1501 ggtgtcggaa aaacagcggt ggtggaagga ttggctcagc gaattgtcaa aggagatgtg
     1561 ccggaaggtt tgaaaaacaa aagacttgtt caactcgaca tgtccgcttt ggttgccggt
     1621 gcaaaatatc ggggcgaatt cgaagagcgc ctaaaggcgg ttttgaaaga aattaccgat
     1681 tccgaaggtc agatcattct tttcatcgat gaattgcaca cgattgtcgg cgcccgagct
     1741 gcggaaggct caatggatgc cggtaatatg attaaaccca tgcttgcacg cggtgaatta
     1801 catctgatcg gtgcaacaac cttaaatgaa tatcgaaaat atatcgagaa agatgcggcg
     1861 ctcgaacgtc gattccagac cgtatttgtc gatgaacctt cggttgaaga tactatcagt
     1921 attctacgag gcttgaaaca gcgctacgaa gtccatcacg gcgttcgaat taccgatgct
     1981 gccgtcattg cagcggcgac attatccaac cgatacatta ccgatcgtca attgccggat
     2041 aaagccattg acttaatcga tgaagcagcg gctcgattac gaacggaaat agattccaaa
     2101 ccgcagattt tggacagtgt tgatcgtgaa atcatgcagc ttgagatcga aaagcaagcg
     2161 ctccaaaagg agcatgatca agcttccaaa gatcgtttgg caaaactgga agaagaatta
     2221 gccaacctga aggaaaaatc aaaccagttg acagcccggt ggcataccga aaaagaagca
     2281 attcaaaacc tgaaaacagt caaagaaaag ctggaatcat tgcacattca gattgaacag
     2341 gcggaacgag ccaacaattt ggaattagct gccagattac gctatggcga attaccggct
     2401 ttggaaaaac aacttgccgc ttatgaagag aagttaaagg aaatccaaaa agagggtgcc
     2461 cttctcaaag aagaggtgga tgaagaagaa attgctcagg ttgtcgcgaa ttggaccgga
     2521 attccggttt ctcgtttgat ggaaagcgaa aaacagaaac tggtgcatat ggaagaaaga
     2581 ttgcatcaac gggtagtcgg tcaagatgaa gcggtaaaag cagtcagcaa tgcggttcgc
     2641 cggtcccgtg ccggacttca ggaccccaac cggccgatcg gttctttcat ctttttaggt
     2701 ccgacaggcg tcggaaagac cgaacttgcg aaggcattag ccgagttttt gtttgatgat
     2761 gaacgcgcca tgatccga
//
LOCUS       DAOMIV010000274         2771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_39545_length_2771_cov_3.300810, whole genome shotgun sequence.
ACCESSION   DAOMIV010000274 DAOMIV010000000
VERSION     DAOMIV010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39545_length_2771_cov_3.300810"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PKV59_06790"
     CDS             complement(<1..1317)
                     /locus_tag="PKV59_06790"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HQP46496.1"
                     /translation="MKRFIFFSVFIAGFSSMAIEMTASRMIGNIFSSANLIWACVIGS
                     IMIYMAIGNWLGGIFSDKYPDLFHYLILIFISGIFCCLIPLFSRPLLLKGAQAMDQMT
                     LRWVLILFLILLLILFIPMTLLGMIIPFAIKLLNTYYPFRGKVNGQLLAVSTVGSFIG
                     TMLPSFILIPSVGTNRTFFVIGFLLLLAGAIGQLTNIGSDKKAEKLMIFCIFLPILLF
                     FLFPSKIKQTKGQIYEKESVYNYIEVVENSGYTILRLNDGQGIQSIYHPVIDNYYGPW
                     EQILTAPFFQPAPIDPGNIRRAAILGNAGGTSAKQLLKVFPNVIVDGFEIDPEITKVA
                     KEFFDLPSERMNIISTDGRIGLQNSDDQYDIIMVDAYQSPYISVNMCTQEFFQIVFEK
                     LNPEGVLVMNIGRSDDDRTLLGAISETVNRIFPQIFIVDIPNSYNSV"
     gene            1412..>2771
                     /locus_tag="PKV59_06795"
     CDS             1412..>2771
                     /locus_tag="PKV59_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQP46497.1"
                     /translation="MRLVSVSEMRQIENQAFGAGISSDHMIQTVGLKIANILDSDCSD
                     KNKMVLGLIGKGNNGSDTLAILTYLAQKGWETFAWLLSKRESEDRYLKDFLNVSGKAV
                     DYENDTEESQLDNWLKQSSIILDGIYGIGFHLPLGEAFRPIFRKINQRKNPSLIYAID
                     CPSGMDCDTGKIDPDCIRANRTICIEAVKIGEVLPPANLLVGELETISLDLPQDLTVY
                     QTIHRTVLDSEVVRMFLPIRPEDGNKSTFGTVTIVGGSEKYIGAPKLSGIAAFKAGCG
                     YVNMVAPEIIRNLNAGIFSQAIWQPADFNSDEGIEIALHSIRDMSKKKGSLVIGPGLG
                     QSPKVFDLVHRILISWKENPVPLLMDADGLNALPHIPDWQKNIPDCFVITPHEGEMAR
                     LCNMTVDEIKTNRIQIAEQKSKEWKTVIVLKGAFTVIASPDGYTAVMPYANSILAKAG
                     SGD"
BASE COUNT          885 a          541 c          571 g          774 t
ORIGIN      
        1 aacagaattg tatgaattcg gaatatctac tataaatatt tgcgggaata ttcggttaac
       61 cgtttcgctg atagctccga gcaaagttcg atcatcgtca gaccttccga tattcattac
      121 taaaacgccc tccggattga gcttttcgaa aacaatttga aaaaattcct gtgtacacat
      181 attgactgaa atatatggag attgataagc atccaccatg ataatatcat actgatcatc
      241 actattttgc agtccgattc gtccgtccgt tgaaataata ttcattcgtt ccgaaggtag
      301 atcaaaaaat tctttcgcga ctttggttat ttcgggatca atttcaaatc catccacaat
      361 tacattagga aagactttca gaagctgctt agcggaagta ccgccggcat ttcccagtat
      421 cgcagctctt cgaatatttc ccggatcgat cggagcgggt tgaaaaaagg gcgcggtcaa
      481 aatttgttcc catggtccgt aataattatc gatcaccgga tgataaatgc tttggattcc
      541 ttgtccgtca tttaatctca atatggtata tccggaattt tcaacaacct caatataatt
      601 atagactgat tccttttcat aaatctggcc ttttgtttgt tttatttttg aaggaaacag
      661 aaaaaataat aaaatcggca aaaaaataca aaagatcata agtttttccg cttttttatc
      721 ggatcctata ttcgttaatt gaccgatcgc tccggctagc aataacaaga agccgatgac
      781 aaagaaagtt ctgttggttc cgacagatgg aatcaaaata aacgacggaa gcatcgttcc
      841 gatgaacgac cccactgtag agacagcgag taattggccg tttactttac cacggaacgg
      901 atagtaagta ttcaacaatt taatcgcaaa agggatgatc attcctaata atgtcatcgg
      961 gataaaaagg atcagaagaa gaattagaaa aagaataagg acccaacgaa gtgtcatctg
     1021 atccattgcc tgagcccctt ttaataagag aggtcttgaa aagagcggga ttaggcaaca
     1081 aaaaatccct gaaataaaaa ttaatatcaa atagtgaaaa agatccggat atttgtctga
     1141 aaaaatacct cccaaccaat tcccaattgc catataaatc ataattgagc cgatcacgca
     1201 tgcccaaatt agatttgcgg aactgaaaat atttccgatc attcgggaag ctgtcatttc
     1261 aatagccata gaagaaaagc cggcgataaa gacggaaaag aaaataaaac gcttcattcc
     1321 tgtattttac cttttaacga attagaataa agacacgttc aattaagaat cggaccggtc
     1381 attttataga aatcgatttt gcgaggatgc catgagatta gtatcagttt cagaaatgcg
     1441 ccaaattgaa aaccaagcgt ttggtgccgg tatatcatct gatcatatga tccaaaccgt
     1501 cggattaaaa attgcgaata ttttagattc cgattgttcc gataaaaaca agatggtttt
     1561 agggctgatc gggaaaggaa acaacggcag cgacactttg gcgatattaa catatttggc
     1621 tcaaaaaggt tgggaaacct ttgcttggct gctctcaaaa agagaatcgg aagatcgtta
     1681 tctcaaagat tttcttaatg tatcgggaaa agccgttgat tatgaaaacg atacggaaga
     1741 gagtcaactt gataactggt taaagcaaag ttcgattatc ttagacggaa tttacggaat
     1801 tggctttcat cttcctttgg gagaagcctt tcgtcccatt tttcgaaaaa taaatcaacg
     1861 taagaatcca tctctaattt atgcaattga ttgtccatcc ggaatggatt gcgataccgg
     1921 aaaaattgat cccgattgta ttcgtgcgaa tcgcacgatc tgtatcgaag ccgttaaaat
     1981 aggtgaggtt ttaccgccgg cgaatctgct ggtgggggag ctcgaaacga tatcccttga
     2041 tttacctcag gatctaacgg tttatcaaac aattcatcgc acggtattgg actcggaagt
     2101 cgtccgtatg tttctgccga tcagacccga ggacggaaat aaaagcacat tcggtaccgt
     2161 gacaattgtc ggcggatcag aaaaatatat cggagctccc aaactctccg gaatcgctgc
     2221 attcaaagcc ggctgcgggt atgttaatat ggttgccccg gagattatca gaaatctgaa
     2281 tgccggtatt ttttctcagg cgatctggca accggctgat tttaactccg atgaagggat
     2341 cgaaatcgct ttgcattcaa tccgtgatat gtcgaagaaa aaaggctcgt tggtcatcgg
     2401 tcccgggttg ggacaatctc ctaaggtatt cgatcttgtt catcgtattc tgatttcatg
     2461 gaaagaaaat ccggtaccgt tattaatgga tgctgacggg ttgaatgctc ttccccatat
     2521 ccccgattgg caaaagaata ttcccgattg ttttgttatt actcctcacg aaggtgaaat
     2581 ggctcgatta tgtaatatga ccgtcgatga aattaagacg aacagaattc aaattgccga
     2641 acaaaaatcc aaagaatgga aaaccgttat tgttctaaaa ggtgcattca ctgtgatcgc
     2701 ttcgccggac ggctataccg ctgtaatgcc atatgcaaat tcaatcttag cgaaagcagg
     2761 cagcggagat a
//
LOCUS       DAOMIV010000275         2771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_39547_length_2771_cov_2.937040, whole genome shotgun sequence.
ACCESSION   DAOMIV010000275 DAOMIV010000000
VERSION     DAOMIV010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39547_length_2771_cov_2.937040"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PKV59_06800"
     CDS             complement(<1..286)
                     /locus_tag="PKV59_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46498.1"
                     /translation="MKKFLPLVLTILILFCFFSVQAEENVLNVLTYSSFAVSEDVIKD
                     FEAENNVKVQFIEGGDTPSIVNRAILTKNSPIADVIVGMNDVLLARVLKNE"
     gene            complement(363..923)
                     /locus_tag="PKV59_06805"
     CDS             complement(363..923)
                     /locus_tag="PKV59_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46499.1"
                     /translation="MTCDLQFIQSINQGFVEILGKEDLDDFRLFLKEPSESESCMRYL
                     SMMQCYLSEKFGYFESEGIAISIGKNAYRPIKIAFESVLHFQEVDFRLLPHHKRIKTA
                     LVRLIERVYKPVGIPIMSLDTEETDSWAIKPEGPIRMFPVQILNGILQEMLLDVSGGH
                     FFPVEVQEDEQRNMIILTFATSPIGH"
     gene            complement(1036..1887)
                     /locus_tag="PKV59_06810"
     CDS             complement(1036..1887)
                     /locus_tag="PKV59_06810"
                     /inference="COORDINATES: protein motif:HMM:NF025840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46500.1"
                     /translation="MDIEETQPIRPKRGNPYVTFPDFSPSVPAEPGIAVPTELNRESE
                     SRFVPPPYPPVPVSPEMARALSVEIKSRKIQFLTHFTRLENLASILTYGLLPPNQLKS
                     EGRTRFVRFNPPYLPGYWQNAISFNISFPNYRLFYNLQEQRGYDWVVLLFDINILLSQ
                     PFYYFIYPAANLIHTPIFATEISPKLQTFEAFEELFQDTENVRRAFLQIPDCYPTHPQ
                     SEVLTFQPVSVNALLEVHFYNDYKFNQWFMQNTALAMTMDKNIWQVSLEFFSPRCDYL
                     NWKSTQR"
     gene            complement(2032..>2771)
                     /locus_tag="PKV59_06815"
     CDS             complement(2032..>2771)
                     /locus_tag="PKV59_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46501.1"
                     /translation="SINWREYETEPFDEKTVQSRPQYADAKYEQPPEWMSDSGSVRSR
                     ESDFLDYIYREGIVKIRANKPLDVYAAPDVTEEDFVAMCKGKVAEASQNEKDKLAKAY
                     ENIVDRMETKIRKAEADVADKTDKVKSKNIEKYGAFGELVIGMFTGRKRSVSTSINKY
                     GQSTNADNALEKAQLVLDELKADLEQQTEEFKKQIAEVEEKWNKIAEETDEVTVTPMK
                     KDIFIDYFGILWLPYYTDENGKFVKAF"
BASE COUNT          797 a          619 c          546 g          809 t
ORIGIN      
        1 tttcattttt aaggacccgt gccagtaaaa catcgttcat cccgacaatg acatcggcaa
       61 tcggcgaatt cttcgtgaga atcgcccgat ttacaatgga aggcgtatct ccgccctcaa
      121 taaactgaac tttcacattg ttttcagctt caaaatcttt aataacatct tcactgacgg
      181 caaaagagct gtaggtcaaa acattcagaa cattctcttc cgcctgaacg gaaaagaagc
      241 aaaataagat caaaatggta agaactaatg gtaaaaattt tttcatgata gactccttat
      301 tccttatttc cgaaaaacct tccgaactga ataatttcac tgtccgacaa aaatccggct
      361 atttaatgac cgatcggact tgtggcaaaa gtcaggatga tcatattccg ttgctcatcc
      421 tcctgaactt caaccgggaa aaaatgcccg ccggaaacat ccagcagcat ttcctgaaga
      481 attccgttta agatttggac cggaaacata cgaatcggac cttccggttt aatcgcccag
      541 gaatctgtct cttccgtatc gagagacatg atcgggatac ccaccggttt ataaactctt
      601 tcgatcagcc gcaccaaagc ggttttaatc cgtttatgat gaggaagcaa ccgaaagtcg
      661 acttcctgaa aatgaagaac cgattcaaat gcaatcttaa tgggacgata ggcatttttc
      721 ccgatactta ttgcgatccc ttccgattcg aaataaccga acttttccga taaatagcat
      781 tgcatcatcg acaggtatcg catacaactt tccgattcgg atggttcttt caaaaacaat
      841 cgaaaatcat ccaaatcttc ttttcccaga atttcaacga aaccctgatt aatggattga
      901 ataaattgca gatcacaagt cattgatatt tattattctc cgggaaattg tttttcacac
      961 ccgccattat accaatacct ttcaaagaat cggccggaaa tgtatcagaa ttctttataa
     1021 gaaattatga aaaaattatc gttgggtgga tttccaattt aaataatcac aacgcggtga
     1081 aaaaaattca aggctgacct gccagatatt tttatccatc gtcatcgcca atgcggtatt
     1141 ctgcataaac cactgattaa acttgtagtc gttgtaaaaa tgaacttcca aaagagcgtt
     1201 gacacttacc ggctgaaatg tcaacacttc cgattgaggg tgggtgggat agcaatcggg
     1261 aatttgcaaa aaagctcggc ggacattctc cgtatcctga aacaattctt caaatgcttc
     1321 aaaggtttgt aatttcggcg agatttcggt cgcaaaaata ggggtatgaa ttaaattggc
     1381 agccggatag atgaaataat agaacggttg gctcaataaa atattaatat cgaaaagcag
     1441 aaccacccaa tcatatcccc gctgttcctg aagattatag aacaagcgat aattcggaaa
     1501 ggaaatatta aagctgatcg cattttgcca gtatcccggc agataaggcg gattaaagcg
     1561 cacaaatcgt gtccgccctt ccgattttaa ttggttgggc ggaagcagcc cgtaggttaa
     1621 aatggaagcg aggttttcaa ggcgggtaaa atgcgtcaaa aattggatct tgcgactttt
     1681 tatttcgacg gataatgctc ttgccatttc cggcgaaaca ggcaccggcg gataaggagg
     1741 cggtacaaat cgactctccg attccctgtt taattccgtg ggaaccgcaa taccgggttc
     1801 ggccggcacg gagggcgaaa aatcggggaa tgtcacatac ggattcccgc gtttgggtct
     1861 gataggttgg gtctcttcga tgtccatata tctatattgt aatttgaaaa tcacccgatt
     1921 ttttgaatcg ccacggattc gagacacatt ccttataaaa ataaatccgc tccggttgat
     1981 aaggaagcgg attttgaatg gttttatgaa ccgaaagaga atgccggcaa ctcagaaagc
     2041 ttttacgaat tttccgttct cgtcggtata gtatggaagc cataagatgc cgaaataatc
     2101 gataaagata tcttttttca tcggggtgac cgtcacttcg tccgtttctt cggcaatctt
     2161 attccatttt tcttccacct cagctatttg ctttttaaat tcctcggttt gctgctcaag
     2221 gtcggctttc agctcatcca acacaagttg ggctttttcg agtgcgttat ccgcattggt
     2281 cgattgccca tatttgttga tggaggtaga aacggatcgt tttctgccgg taaacattcc
     2341 gatgaccagc tcaccgaatg caccatattt ttctatattc ttgctcttca ctttatcggt
     2401 tttgtcggct acatcagctt ccgctttgcg tattttcgtc tccatacgat cgacaatatt
     2461 ttcgtatgct ttagccaact tatctttttc gttttgtgaa gcttcagcca cttttccttt
     2521 acacatcgca acaaaatcct cttcagtcac atccggagcg gcataaacat ccaacggttt
     2581 gtttgcacga atctttacga ttccctctcg atagatataa tcgagaaaat cgctctctcg
     2641 acttcgaaca ctgcctgaat ccgacatcca ttcaggcggc tgttcatatt tcgcatcggc
     2701 atattgcggt cgactttgaa cagttttttc gtcgaaaggt tccgtttcat attcccgcca
     2761 attaatggaa a
//
LOCUS       DAOMIV010000276         2761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_39754_length_2761_cov_4.439763, whole genome shotgun sequence.
ACCESSION   DAOMIV010000276 DAOMIV010000000
VERSION     DAOMIV010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39754_length_2761_cov_4.439763"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PKV59_06820"
     CDS             <1..394
                     /locus_tag="PKV59_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQP46502.1"
                     /translation="EHRDLTYNRKGTPHFAKPFLPWIYVFGKSDQFIPDIPLYDGTDL
                     TSEGFSATVYSIPGHTKGSVAVLTIGGDLFCGDLLVCRNRVVMNTLLDDREDAAASIR
                     KLRNLPAHMVYPGHGKPFPMNDFTKEHF"
     gene            complement(485..1381)
                     /locus_tag="PKV59_06825"
     CDS             complement(485..1381)
                     /locus_tag="PKV59_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQP46503.1"
                     /translation="MQTQSQLTIVIPAFNEEKSLQTFLPQVLNYCDENHFRLIVVNDA
                     STDRTAEVLDHFQNQFPEIMKIVNHKVNKGYGGALSTGLKQAETPFVVTLDADGQHRL
                     EDVTALMQEQQRTNADLVIGRRPEKINGDLQQSYRSLGKKLIRRVAKMMMDVPVRDLN
                     SGLKLYNTELVQKYLPLCPESMAFSEIITLIFLQNKHLVTEIPVEVVPRKEGKSSVTT
                     MTALETIIQIFNIIMLFSPLRIFLPVGTFLMAVGILWAIPFLIHGNGLSTTALLFITT
                     GLILVMMGLIAEQLAQIRRKDF"
     gene            complement(1470..1553)
                     /locus_tag="PKV59_06830"
     tRNA            complement(1470..1553)
                     /locus_tag="PKV59_06830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1517..1519),aa:Leu,seq:gag)
     gene            1727..2725
                     /gene="plsX"
                     /locus_tag="PKV59_06835"
     CDS             1727..2725
                     /gene="plsX"
                     /locus_tag="PKV59_06835"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQP46504.1"
                     /translation="MAIVLDAMGSDDCPQPEIEAAIYAAKNLNESIILVGHKDIIREH
                     VSESDFAGLPIEIVHAEDILEMGEKAVKSAQLKPNNSMAVGLRLVKEGKAEAFLTAGN
                     TGAAMFNSLRELGRIRNVARPALTALFPTAKGFSVVIDIGANSDCRPEYLLQFAVMGS
                     IYAEAMQKISKPKVGLLNNGEEPGKGNELMRTTYPLLESSKKINFIGNVEAKEVFAGK
                     ADVVVTDGFTGNIFLKTSEATAKMLTDILKEQLMPSIASGGDNSLAKNILSAVKSRMD
                     PNAVGAAPLLGIDGLVFVGHGRSNSTALISAIKVVREALNVDLVGTVRQSIQEELA"
BASE COUNT          767 a          547 c          686 g          761 t
ORIGIN      
        1 tgaacatcgc gatctgactt ataaccggaa aggaacgccc cattttgcaa aaccgttcct
       61 gccgtggatt tatgttttcg gaaaatccga tcaatttata ccggatattc ccttgtatga
      121 cggaacggat ttgacatccg aggggttctc ggcaaccgtt tactcgattc ccggtcacac
      181 aaagggatct gtggcagtat taacgattgg cggcgatttg ttttgcggag atttattggt
      241 ttgccgaaat cgcgtggtga tgaacacgtt gttggatgat cgcgaagatg cggctgccag
      301 catccgaaaa ttgaggaatt tgccggctca catggtttat cccgggcatg gcaagccttt
      361 tccgatgaat gattttacga aagaacattt ctgattgact gctacaaagc acaatcaatc
      421 aaccggcata tttcagagaa cagattcttg ataaggatca agagatcttt tctctgattc
      481 tatttcaaaa gtcttttctg cgaatttggg ctaactgttc tgcgatgaga cccatcatga
      541 ccagaatcaa tccggtcgta ataaacagta atgcggttgt gctcaatccg tttccgtgga
      601 tcagaaaggg aatcgcccat aaaattccca ccgccatcag aaatgttccg acgggcagaa
      661 atattcgtaa gggactgaat aacataatga tattaaaaat ctggatgatt gtctcgaggg
      721 cggtcatggt tgtgacggaa cttttgcctt cttttcgcgg aactacttcg acgggaattt
      781 cagtgaccaa atgtttattt tgcaagaaga tcagagtgat aatttccgaa aaagccatgc
      841 tttcggggca gagcggaagg tatttttgaa ccagttccgt attatataat ttaagcccgc
      901 tgttcaggtc tcgtacggga acatccatca tcattttggc gactcgccgg atcaattttt
      961 tccccaatga gcggtagctc tgctgaagat caccgtttat cttttccgga cggcgaccga
     1021 ttaccaaatc ggcattggtg cgttgctgtt cctgcatcag ggcagtgaca tcctccagac
     1081 gatgctggcc gtcagcgtcc aaagttacga caaaaggtgt ttcagcctgc tttaagccgg
     1141 tagagagtgc gccgccgtat cctttgttca ctttatgatt tacaattttc atgatttccg
     1201 ggaattgatt ctgaaaatga tccaaaactt ctgcggtacg gtcggtagaa gcgtcattta
     1261 ctacgatcag ccggaaatgg ttttcgtcac agtaattgag cacctgaggc agaaatgttt
     1321 ggagagattt ttcttcgtta aatgccggaa tgacaatggt tagttgtgac tgggtctgca
     1381 tgaaatgatc ttaatcgaaa atcgacgatt ttacaataaa aaaagttatg actcggttta
     1441 aaaaaaatcg cattcataag cgatttttgt gcgctggggg ggaatcgaac ccccacgacg
     1501 aagatgtcac atggccctca accatgcctg tctgccaatt ccagcaccag cgcgttagct
     1561 tttgtattat aatcaggtac gcagcgttgt caaggaaatg agacgcaaac tgatcttatc
     1621 ggatcaggat ttcgctgtgg gatgaatgat taagaataag ttgaaaatag gatgaaaaac
     1681 ctgtgattcg gtttttttaa atagattttt actgtcagga gacaatatgg ctattgtttt
     1741 ggatgcaatg ggcagcgatg attgccccca accggagatt gaagccgcaa tctatgcggc
     1801 gaaaaatttg aatgagagca ttattctggt aggtcacaaa gatattattc gggaacatgt
     1861 ttcggaaagt gactttgccg gattaccgat cgaaatcgtt catgcggaag atatccttga
     1921 aatgggtgaa aaagcagtga aaagtgctca actgaaaccg aataattcaa tggctgtcgg
     1981 tctgcgattg gttaaagaag gaaaggcaga agcgtttttg accgccggaa atacaggcgc
     2041 ggccatgttt aatagcttgc gtgaattggg acggatcaga aacgtcgccc gtccggcact
     2101 aaccgcactg tttccgaccg caaaagggtt tagcgtagta atcgatatcg gagcgaattc
     2161 cgattgccga ccggaatatc tgctgcaatt cgctgttatg ggctcgattt atgcagaagc
     2221 gatgcagaag atttccaagc ctaaagtcgg tctcttaaat aacggcgagg aacccgggaa
     2281 gggtaacgaa ctgatgcgga cgacttatcc cttattggaa tcttcgaaaa aaatcaattt
     2341 catcggaaat gtggaagcga aagaagtttt tgccggaaaa gcggatgtgg tggtgactga
     2401 cggatttacc ggtaacatct ttttgaagac ttcggaagcg actgcgaaaa tgttgactga
     2461 tattttgaaa gaacaattaa tgccttcgat agcatccggt ggagataact ctttagctaa
     2521 gaatatccta tccgccgtaa aatcaaggat ggatccgaat gcggtggggg ccgcccctct
     2581 gttggggatt gacgggttgg ttttcgtcgg gcatggccgt tcaaacagta cggcattgat
     2641 cagtgctata aaagttgtac gggaagcttt gaatgttgat ttggtcggga cggttcggca
     2701 atccattcag gaagaattgg cataaattaa acagaggaat gattcatgta tatttacact
     2761 a
//
LOCUS       DAOMIV010000277         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40123_length_2745_cov_4.827881, whole genome shotgun sequence.
ACCESSION   DAOMIV010000277 DAOMIV010000000
VERSION     DAOMIV010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40123_length_2745_cov_4.827881"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PKV59_06840"
     CDS             complement(<1..751)
                     /locus_tag="PKV59_06840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQP46505.1"
                     /translation="MIPEIKDLCTFAEQAGVITIDGFGKTHQVTQKGIQDPVTEIDQK
                     SENFLIRQINHFYPGHTILSEESGLHNGTITAAEHVWYLDPLDGTMNYSHSVPFYSIS
                     IGYAFKGELVLGVIYDPMRHECYCAEKGKGAYLNGTKIQVSRETDLRYALLATGFNMK
                     IPETGRDNFPLFKHFMQTTAGVRRMGSAALEIAYTAAGRLDGMWEMHLSAWDVAAGFV
                     IAQEAGAVVSNLKGDPNCFHVPFEFLIANPIL"
     gene            complement(912..1532)
                     /locus_tag="PKV59_06845"
     CDS             complement(912..1532)
                     /locus_tag="PKV59_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQP46506.1"
                     /translation="MGTVSTDLIKQLRESTGAGILECRKALENANGDLKAAMESLREK
                     GLATALKRSSRAASEGVIEVYTHGGGRVGVMLELNCETDFVGRSESFRYLAHELALQI
                     AASSPLYISDEDIPQSVLDNEAKIAEAKAREEGKSEAIIPKIVEGYLKKYKDEVVLLN
                     QIYIRDESIKVQDLINQNIAALGENIIVRRFARYALGESSDMDEAE"
     gene            complement(1537..2433)
                     /gene="rpsB"
                     /locus_tag="PKV59_06850"
     CDS             complement(1537..2433)
                     /gene="rpsB"
                     /locus_tag="PKV59_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQP46507.1"
                     /translation="MTSVISMKALLESGVHFGHRTNKWHPAMRPYIFTERNGIHIIDL
                     QQTAKSINTVYPLVRDTVQSGKEILFVGTKRQAQETIRDEAIRCGMPYVTERWLGGTL
                     TNWTTIYQRINELEKLEKMKETGEINKLTKKEGLMIEREIARLELRLSGLRNMKTLPG
                     LLFVIDVMREATAIHEANLKNIPVIAMVDTNCDPTNVDYVIPSNDDAIRAIKLIVSKI
                     ADAVIEGKALRGEGNFDEGSFSDDSKPNNAKLLYNDTGDMEDEDLFGASTLAKLGNMK
                     AAKQDAGEDSNGGEYDESEEEE"
BASE COUNT          720 a          636 c          522 g          867 t
ORIGIN      
        1 gaagaatcgg attggcaatc aaaaattcaa agggcacatg aaagcaattc ggatcgcctt
       61 tgagattact aaccacagcg cccgcttcct gcgcgataac aaatccggcg gcgacatccc
      121 atgccgataa atgcatttcc cacattccat ccagcctgcc cgccgcagtg tatgctattt
      181 ccaaagcagc cgaacccatc cttcgtacac ccgctgtagt ttgcataaaa tgcttgaaca
      241 gagggaaatt atctcttccc gtttccggaa tcttcatatt gaatcccgtg gcaagcaagg
      301 catagcgcaa atctgtttcc cgtgaaacct gtatttttgt accgtttaaa taggcaccct
      361 ttcctttttc ggcacaatag cattcgtgtc gcatcggatc gtaaataact cccaatacca
      421 gctccccttt gaaagcataa ccgatggaga tgctgtaaaa aggtacgctg tgtgaatagt
      481 tcattgtccc atccaacggg tccaaatacc aaacatgttc cgcagcggtt atcgttccgt
      541 tatgaaggcc gctttcttcg ctgagaatgg tatgccccgg atagaaatga ttgatttgtc
      601 gaataagaaa attttcggat ttctgatcga tttcggtaac cggatcctgg attccttttt
      661 gagtgacttg gtgtgtttta ccaaatccat caatggtgat cactccggcc tgttcggcaa
      721 atgtgcaaag gtctttgatt tcgggaatca tgtctttcgt tcgctttcgg cttattattg
      781 accattatac tctgcttttc gaggtaactg tttcaagttt ctatgaatgt tttcggtaat
      841 tataaaaaaa aaaccggttt ccccaattaa aaaccggttt tctaaagttt atattgataa
      901 agttggaatg attattctgc ttcgtccata tcggatgatt cacccaacgc ataacgcgca
      961 aaccgtctga caatgatatt ttcacctaaa gcagctatat tctgattgat taaatcttga
     1021 accttgatgg attcatcccg aatgtaaatt tgatttaata agacaacttc gtctttatat
     1081 tttttcaagt aaccttccac aatcttcgga ataatcgctt cactttttcc ttcttcacga
     1141 gctttggctt cggctatttt tgcttcatta tccaaaacac tttgcgggat atcctcgtca
     1201 ctgatataaa gcggagaaga tgcggcaatc tgtaaggcta attcatgagc caagtaacga
     1261 aacgattccg aacggccgac aaaatcggtc tcacaattca attccaacat aacaccaact
     1321 cgaccgccac cgtgagtata aacttcaatg acaccttctg aagcggctcg actggatcgt
     1381 tttaaagccg ttgcaagccc tttttctcgt aaggattcca tcgctgcttt cagatcgccg
     1441 tttgcgtttt ccagtgcttt tcgacattca aggatgcccg caccggtact ttcacgtaat
     1501 tgtttaataa gatctgtact gactgtaccc atttaattat tcctcttctt cgctttcatc
     1561 atattcgccg ccattcgagt cttcgccggc atcctgttta gcggctttca tgttgcctaa
     1621 ttttgccaat gtcgatgctc cgaataaatc ttcatcttcc atgtctccgg tatcgttata
     1681 caagagtttg gcattattgg gcttagaatc atctgagaag gatccttcat cgaaattacc
     1741 ttcaccgcgt aatgcttttc cttcaatcac cgcgtcggcg attttgctga caatcaactt
     1801 gattgcccga atggcatcat cattggaggg gatgacatag tcaacattgg tcggatcgca
     1861 attggtatcg accattgcga tgacgggaat attcttaaga ttggcttcat gaattgctgt
     1921 ggcttcacgc ataacatcaa tgacgaaaag caaccccggt aaggttttca tatttcgtaa
     1981 accgctgagt cgaagttcca aacgagcaat ttcacgctcg atcatcaagc cttctttttt
     2041 agtgagcttg ttaatctccc cggtttcttt catcttttcc agcttttcaa gctcattgat
     2101 acgctggtaa attgttgtcc agtttgttaa tgtgccgccc agccatcgtt cggttacata
     2161 aggcattccg caacgaatcg cttcatcccg gatggtctcc tgtgcctgac gttttgtccc
     2221 gacaaaaagg atctcttttc cggattgtac ggtgtcccgt accaatggat aaaccgtgtt
     2281 gatacttttt gcggtctgct gtaaatcaat aatatggatc ccgttccgct cggtaaaaat
     2341 ataaggtcgc atggcgggat gccatttgtt tgttcgatgt ccaaaatgga caccactttc
     2401 caataatgct ttcatggaaa taacactggt cataataaaa actccttttg cgttttagcc
     2461 tctgctttcg tcaatttctt tttagtcaca accttatcgg gttgcacgca gcaaaaagat
     2521 cggaaagcat gtgttttata ttgccgcatg attttatcat ggaatctaaa ttaaataaaa
     2581 caaaactgat ggaaaaatgc tttttccata gacttagaga tcatataaat cgatttttcc
     2641 atcagtagtc ggagcttttt attccctatg attgtaaaat ccggttgatt ttgaaaagat
     2701 cttctgaccc tattattgca aaaaatgtca ttagaatccg gacat
//
LOCUS       DAOMIV010000278         2745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40129_length_2745_cov_3.878067, whole genome shotgun sequence.
ACCESSION   DAOMIV010000278 DAOMIV010000000
VERSION     DAOMIV010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40129_length_2745_cov_3.878067"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..495
                     /locus_tag="PKV59_06855"
     CDS             178..495
                     /locus_tag="PKV59_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46508.1"
                     /translation="MDNKMSGKILLLTVIAGRKQKDSLLTALLETGVQSTNILYGKGS
                     VSESTLKNIFGFIPEENKVIIICLVSDKKAESVLNLLIEKFHFNLPNTGIGFTIPIEK
                     ILF"
     gene            563..895
                     /locus_tag="PKV59_06860"
     CDS             563..895
                     /locus_tag="PKV59_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46509.1"
                     /translation="MEQKSNIKALIIIVNAGFSNEAIKIARESGATGATILNARGESL
                     RHEVFMGISVDSEKEIILCLVEEIVADKIMLTIQEKAGIKTPAHGICYTMGVEKIIGI
                     NSFSSQAN"
     gene            1105..2169
                     /locus_tag="PKV59_06865"
     CDS             1105..2169
                     /locus_tag="PKV59_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQP46510.1"
                     /translation="MAFNPLNWLMGLFSLDIGIDLGTANTLVYVRGKGIVINEPSWVA
                     VEKRTRRPVAIGAQAKEMVGRTPANLLVVRPMRDGVISEFEITQAMLSYFIGKAHQQT
                     IAPFPRPRVVIGIPCGATDVERRAVYDAAMAAGAREIYLIEEPKATAIGIGLPINEIR
                     GTMVVDIGGGTTEVAILSMNEIVVSRSLRVAGDEIDQDILNYIRNRYNLYIGERMAEQ
                     TKISIASAYPLPVERTMVIRGRNLVTGLPEAIEVSSVEIREAISGSIQVIVDTVKDAI
                     DEAPPELVADLIETGICLSGGGSQILGMADRLNDELNIRVWVAEDPMTCVARGAGMVL
                     ENSSTIEQFAASLDRFTPKK"
     gene            2266..>2745
                     /gene="mreC"
                     /locus_tag="PKV59_06870"
     CDS             2266..>2745
                     /gene="mreC"
                     /locus_tag="PKV59_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQP46511.1"
                     /translation="MNNVKKNWQKIVAILIISGVIFLALSGFLTQSLSGLIDPIIQVQ
                     KWTAERIQTVYEFFTIPRDVTQLRAENQALQEQVSLLQTEIIQLQQDLKEADILYSLL
                     GFARGRPEETYIAAAVIGVDPSPFLQYILIDKGSDDGIAYNMPVVTEKGLVGRISAVT
                     "
BASE COUNT          874 a          462 c          630 g          779 t
ORIGIN      
        1 gattcttggt atcatttata acctgcgctt gaaaaaggca cagaaaatta cggcggaaga
       61 agaactggaa agccttgatg atcttatcta ccaaaaagaa agtatataaa ttgagttgac
      121 aaaaacaaaa taaagagttt aatcaaatag cctgaagaag ttttaaagga attgagaatg
      181 gataataaga tgtcagggaa aatattgttg ctgactgtga ttgcggggag aaaacaaaaa
      241 gactccttgt tgacagctct attagaaacc ggtgtacagt cgacaaatat attgtacgga
      301 aaaggttctg tatctgaaag taccttaaaa aacatatttg gttttattcc ggaagaaaat
      361 aaggtaatta ttatatgttt ggtttccgat aaaaaagctg aatcggttct gaatcttctc
      421 attgagaaat ttcattttaa tttacctaac acagggatcg gtttcacaat tccgattgaa
      481 aaaatattat tttagtgaaa aataagatca atagggtggc gcaaatcgga tttgatagaa
      541 atcatcaaaa ggaatgggtc aaatggaaca aaaaagtaat attaaagctc tgattattat
      601 tgtcaatgca ggtttttcaa atgaagctat aaaaattgcg agagaatccg gagctaccgg
      661 cgctacgata ttgaatgcca gaggcgaaag tctgcgtcat gaagttttta tgggaatttc
      721 tgtcgattcc gagaaggaaa ttattctttg tttggtagaa gaaatagttg cagacaaaat
      781 catgttgacc attcaagaaa aagcaggtat taagactccc gcacacggta tttgttatac
      841 gatgggagtg gaaaaaatca tcgggataaa ttccttttca tctcaagcga attaaatggc
      901 aaaaccataa attttatgat gctgttttcg tgttatacag cagaatactg aatgttgtaa
      961 aaaccaaatg acgatttggt ataatcgaaa tgttatacag agtaacgaac gtcgattact
     1021 ttctgaatga ttaaatagcg gaagaaataa aaaagtaatc gaatttggga atagaaacga
     1081 tactggatct ctcggagcaa taatttggca ttcaatccat taaattggtt aatgggcttg
     1141 ttctcattag atatcggaat tgatttgggg acagcaaata cacttgtcta cgttcgcggc
     1201 aaaggtattg tgatcaatga gccatcctgg gttgccgtag agaagaggac aagacgtccg
     1261 gttgctatcg gagcacaagc aaaagaaatg gtcggtcgta cgcctgcaaa tttactggtt
     1321 gtgcgtccga tgcgtgacgg tgttatttcc gaattcgaaa taactcaggc aatgctcagc
     1381 tattttattg gaaaagccca tcaacagacg attgcacctt tccctcgtcc cagagtggtg
     1441 atcggaatcc cttgtggagc gaccgatgtc gaacgccgtg ctgtttatga tgcggcgatg
     1501 gctgccgggg ctcgagaaat atatcttatt gaagaaccga aagccacggc gatcgggatc
     1561 ggattaccga tcaatgaaat acgcgggacg atggtcgtgg atataggcgg aggcacaacg
     1621 gaagtagcga ttctttcaat gaatgagatc gttgtctcac gatccctgcg tgttgccggt
     1681 gatgaaatcg accaagatat tttaaactat atcaggaatc gatataatct ttatatcggc
     1741 gaaagaatgg ctgaacaaac aaaaatttcg atcgcatccg catatccgct tccggtagag
     1801 cggacgatgg tgattcgtgg caggaatttg gtaaccggtc ttccggaagc tatcgaagta
     1861 tccagcgttg aaattcggga agcgatttcc ggttcaatcc aggtaattgt ggataccgta
     1921 aaagatgcca tcgatgaagc acctcccgaa ttggttgcag atttaatcga aacggggatt
     1981 tgtttatcag gtggaggatc tcagattttg ggaatggcag accggttaaa tgatgaatta
     2041 aatattcgtg tctgggtggc ggaagatccg atgacctgtg ttgcccgcgg agccggaatg
     2101 gttcttgaaa attcatcaac aatcgaacaa tttgccgcaa gcttagatcg atttaccccc
     2161 aagaaataat ttatttgaga cttaattatt tttcaaattt tctttatccc ttaatgttct
     2221 ttctgatctg tacctgttcc cgaattgtta taataattca aagatatgaa taatgttaaa
     2281 aagaactggc agaagatcgt agcgatctta attatttcgg gagtcatatt tttggcttta
     2341 agcggtttct tgacccaatc tttgagtggg ttaattgatc cgattattca agttcaaaaa
     2401 tggaccgccg aaaggattca gacggtttat gagttcttta caataccgag agatgttact
     2461 caactccggg ctgaaaatca agccttacag gaacaagtat ctcttttaca aacggaaatt
     2521 attcaattac aacaagactt gaaagaagcg gatattctgt attctctgtt aggattcgcg
     2581 cgcggaagac cggaagagac atatatcgca gctgccgtaa ttggtgtcga tccgagtcct
     2641 tttttgcaat atatcctgat cgataaagga tccgatgacg ggattgctta taatatgccg
     2701 gttgtaacag agaagggtct ggtcggaaga attagcgccg ttact
//
LOCUS       DAOMIV010000279         2744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40149_length_2744_cov_4.249163, whole genome shotgun sequence.
ACCESSION   DAOMIV010000279 DAOMIV010000000
VERSION     DAOMIV010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40149_length_2744_cov_4.249163"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PKV59_06875"
     CDS             complement(<1..1439)
                     /locus_tag="PKV59_06875"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HQP46512.1"
                     /translation="MEHAKEKDHQPEKIYTPPMPEGLPSISLTENSIQVLTKRYVRRG
                     EDGKPIETIDEMFWRVAYHVALAEKNYGGDVIETAIAYYKLMVNKLFFPNSPTFTGAG
                     TPLGQLAACFVLPISDDMGKKSTGIFQTLRDAALIQQTGGGNGFSFSRLRPKGTIVRS
                     SAGQATGPVGFLRVYDHAFGEIAQGGSRRGANMAVLRIDHPDIEDFICCKTNENSITN
                     FNISVGITDDFMKAVEEDASWDLIYPDLTSPLTKEFDGDVQDAKDQGLPIKVYKTVRA
                     RDLFDLIVNQAHHNGEPGVLFIDQANRFNPVPNLYRLESTNPCGEQWLGPYENCCLGS
                     INLAQHVLEDGSVDWEKLEQSTVLATRFLDDVIDVNAYVPAVPQLKEAALRARRIGLG
                     IMGLADLMFNGGIRYGSEEGQEFASQVMEFIRYHSMKESIRLAEERGAFPAIKGSIYD
                     PDAIKWQIPTPLNPYRHDWNRPETDWEQI"
     gene            complement(1454..1906)
                     /gene="nrdR"
                     /locus_tag="PKV59_06880"
     CDS             complement(1454..1906)
                     /gene="nrdR"
                     /locus_tag="PKV59_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQP46513.1"
                     /translation="MNCPFCGEPKTRVIDTDKDEGNTVHRRRQCPACGKRFNTYERPL
                     LGIPMVVKADNSRQEFSREKLLRGIRIACAKRPVPAEKIEQLVDDIERELQMEGQREI
                     SSRVIGDKVIAGLKSLDQIAYVRYAIVYLQLDDLLSLRKEIDRLLSES"
     gene            complement(2269..2341)
                     /locus_tag="PKV59_06885"
     tRNA            complement(2269..2341)
                     /locus_tag="PKV59_06885"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2305..2307),aa:Glu,seq:ctc)
     gene            complement(2396..>2744)
                     /locus_tag="PKV59_06890"
     CDS             complement(2396..>2744)
                     /locus_tag="PKV59_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46514.1"
                     /translation="LNKVSEFLEDRKGLLIFSVILLLSTMTIFIFSSDWGRLAYLYGF
                     NIYLSLVVLKEHFSCEDSVAGSTVLSGYQTVQWIAFFILFTGTWFLKMYVDGGNIALQ
                     QGLIFKILDWISI"
BASE COUNT          791 a          653 c          537 g          763 t
ORIGIN      
        1 ttgatttgtt cccaatccgt ctcgggtcga ttccaatcat gccgatatgg attcagagga
       61 gtcggaatct gccatttaat cgcatccgga tcataaatcg agcctttgat tgcgggaaac
      121 gcaccgcgtt cttccgctaa tcgaatgctt tccttcatcg aatgataacg gataaattcc
      181 atcacctggg aagcgaattc ctgtccttct tccgagccgt atcgtatacc gccgttgaac
      241 attaaatccg ccaaacccat gatcccgaga ccgatccgtc gcgccctcaa tgccgcttct
      301 tttagttggg ggacggccgg aacatatgcg tttacgtcaa tcacatcatc caagaatcgg
      361 gtggcaagaa cagtgctttg ctctaatttt tcccaatcca cactgccgtc ttccaaaaca
      421 tgttgtgcca gattaatcga gcccagacaa caattttcat aaggacccaa ccattgctct
      481 ccgcatggat tcgtggattc caatcgataa aggttgggaa ccggattaaa tcgattcgcc
      541 tgatctataa acaatacccc cggttcaccg ttatgatgcg cttgattcac aatcaaatcg
      601 aataaatccc gcgctcgcac cgtcttatac accttaatcg gcaaaccctg atctttggca
      661 tcctgcacat ctccatcgaa ctccttggtc agaggagaag ttaaatcggg ataaatcaga
      721 tcccaagagg catcctcttc gaccgctttc ataaaatcat cagttattcc gacggaaata
      781 ttgaaattgg taatcgaatt ttcattggtc ttacaacaga taaaatcttc gatatccgga
      841 tggtcgattc gcaaaaccgc catatttgca ccgcgccgcg atccgccttg ggctatttcg
      901 ccgaaagcgt gatcatatac ccgcaagaaa ccaaccggtc cggtggcctg tcccgctgag
      961 gaacggacaa tcgttccttt ggggcgtaat ctggaaaaag aaaaaccgtt tcccccgccg
     1021 gtctgttgaa ttaatgcggc atccctcaat gtttggaaaa tacccgttga ttttttcccc
     1081 atatcatcgc tgatcggtaa aacaaaacag gcagctagct gtccgagagg tgttccggcg
     1141 cccgtaaatg tcggagaatt gggaaaaaat aacttattaa ccatcaattt atagtaagca
     1201 atcgcggtct cgatcacatc accgccataa ttcttttcgg caagagccac atgataagct
     1261 acccgccaaa acatctcatc gatagtttca atgggtttcc cgtcctcgcc gcgtcggaca
     1321 taacgcttgg ttaacacttg gatggaattt tccgtcagag atatggaagg taatccttcc
     1381 ggcatcgggg gtgtgtaaat tttctcaggt tgatgatctt tttcttttgc gtgttccatt
     1441 caattctctc ctgtcaactt tctgaaagca gacggtcaat ttcttttcga agggataata
     1501 gatcatccaa ttgcaaataa acaatcgcat agcggacata agcaatctga tcaagtgatt
     1561 ttaaaccggc aatcaccttg tcaccgatta cccgggaaga tatttccctt tgtccttcca
     1621 tttgcaattc cctttcaata tcatcgacta actgctcaat tttttctgcc ggaaccggcc
     1681 gctttgcaca ggcaatccga atcccgcgca gcagtttttc ccggctgaat tcctgcctgg
     1741 aattgtcagc ttttacaacc atcggtatcc ctagcagcgg tcgctcataa gtgttgaaac
     1801 gctttccgca tgccggacat tgacgccggc gatgaaccgt atttccttca tctttatcgg
     1861 tatcgattac tcgggttttg ggttctccgc aaaaagggca attcataggt cagataattc
     1921 aattctccga ataaaacaaa aaaaagaggg ttatttttat tgtaagaata cctgctttcc
     1981 gaaataaaaa taaccttaga atatttttga cgattgttaa tcgcttaata aatcactata
     2041 tatggtattt tatctatccg aaatcccttt atatagtgta ggtaatttta gcatgaaata
     2101 tgcattcaaa caagggagca gctgaaaact ttaaggttaa aatttttccg actgtttatt
     2161 atggggcgat aaaggtctta taccgacata atttcatcgt tttaagacaa aaatgtcttt
     2221 caaatttacc gcttttattt gaaaagtatc ggatcattaa agaataagtg cccccagaga
     2281 aattcgaatc ctcgttttgg ccttgagagg gccacgtcct gggccgctag acgatggggg
     2341 caattgaacg gtttttattt tatcatgaat cacggaaaac aataaaaatg agaggttata
     2401 tcgaaatcca atcgagaatt ttaaaaatca aaccttgctg taaggcaata tttccaccgt
     2461 cgacatacat tttcaggaac catgttcccg taaacaaaat aaagaaagcg atccattgaa
     2521 cagtttgata gccagataaa acggtgcttc ccgcaaccga atcttcacaa gaaaagtgct
     2581 ctttgagcac gacgagactc agatagatat taaaaccgta caaataagct aatcgccccc
     2641 aatcggaact gaatataaaa atcgtcatgg tggaaagcag cagaatcacg gagaaaatga
     2701 gtaacccctt acggtcttcc aaaaattccg ataccttatt cagg
//
LOCUS       DAOMIV010000280         2743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40169_length_2743_cov_4.212054, whole genome shotgun sequence.
ACCESSION   DAOMIV010000280 DAOMIV010000000
VERSION     DAOMIV010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40169_length_2743_cov_4.212054"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PKV59_06895"
     CDS             complement(<1..1257)
                     /locus_tag="PKV59_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HQP46515.1"
                     /translation="MKISQPIARINFFTFITSHYNLGKNEKNGEKLSPAYNKEEITIT
                     NFSSDGKAVGKLSDGRVVFVPGTIPGERVLIRYSKSDKRYVQAELIEILSYSPKRTSS
                     RCRYFGTCAHCRLQHLNYFDQTEVKRNILIDQLKRIGKMSHPESWIQPTIPSDPYYGY
                     RKNMIFSVLPDGKLGLQDVSGGMFVADRCPICSEGINELIRQISFEAESGIHAAEFRE
                     GVEGELQIVLRGDADRPPMEIETDLSVSLVYSGPVGSFVLSGNSTLVQEIAGQEICAS
                     EDSPFFPNPEIYDALCQKLIEILPEDRDKPVLNLNCGTGFWAKWFASQGRKVIAQDVG
                     ERSADDFAVNLDAFDSVDLYLGKPESIIPAFRENKPLTVFEAASEGISVATAQALSKT
                     EVPRILYISHDSAILARDTIRLSDSGY"
     gene            1211..2053
                     /locus_tag="PKV59_06900"
     CDS             1211..2053
                     /locus_tag="PKV59_06900"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HQP46516.1"
                     /translation="MNVKKLMRAIGWEIFILLCGSLAGCHPVSNQAIGSESVHVTLPP
                     PQQIQLPSLTPFSAVEISTVTFLPENRSKSATLPAFFQTPTLKVVSGIEPDSKEYDVP
                     LVTQDGLKYKGVKTTLGCTAASVQMVLDYWKKVNPDFKTISAQKIIDINTSQGTFRTG
                     EGLSIENVKDELQNIGYILEIYQNSDKEQLLSVFDQFGPQILLVKTGWVPTGANHLVV
                     LVAYDKETDTVTVNDPWFDHPFTWDWEAFDGIWSLNYSQKENGYLTRSFFNIRPASLS
                     DNSD"
     gene            complement(2050..>2743)
                     /locus_tag="PKV59_06905"
     CDS             complement(2050..>2743)
                     /locus_tag="PKV59_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HQP46517.1"
                     /translation="EDYVGWFWFPEDEGANNYNYDNNTFMPFSGSFGNLNDIWDYSQP
                     LPTQAYVDGFVGHAQSYPLDCETRSAVDFAAYFGFSIDAYQFLTSLPKSDDPNEGFVG
                     NYTDPRGQIPPDSYGVYQEPVAALLRSYGVPAVGYKNMSWERIKYEISNGRPVMVWVA
                     GNTEVGTPQYYTPSNGKSTKVVPYQHTVVVIGYDSQNVTIQDGAMRYTRSINTFLTSW
                     ATLDNRGIIAVY"
BASE COUNT          736 a          678 c          583 g          746 t
ORIGIN      
        1 atatccggaa tcgctcaaac ggatcgtgtc acgtgccaga atcgcactat cgtggctgat
       61 ataaagaatc cggggaactt ccgtctttga taaggcttga gcggtcgcaa cggatatccc
      121 ttccgaggcc gcttcgaaaa cggtcagcgg tttattttct ctgaatgccg ggataatcga
      181 ttcgggcttt ccgagataaa gatccaccga atcaaaagca tccaaattaa ccgcaaaatc
      241 atccgcactg cgttctccga catcctgcgc gatcactttc cgcccctgtg aagcaaacca
      301 tttcgcccaa aatccggtac cgcaattcaa attcaacacc ggcttatccc gatcttccgg
      361 aagaatctcg atcaatttct gacacaaagc gtcgtaaatt tcgggattgg ggaaaaaagg
      421 gctgtcttct gaggcgcaga tttcctgccc ggcgatttct tgaaccaaag tgctgttgcc
      481 ggataaaaca aaagatccga ccggaccgga ataaaccagc gaaacgctca aatccgtttc
      541 gatttccatc ggcggacgat ccgcatcacc tcttaaaaca atctgtagtt caccttcgac
      601 accttctcga aattcggctg catgaatccc gctttcggct tcaaatgata tctgccgtat
      661 caactcatta attccctcac tgcaaatcgg gcaacgatcc gcgacaaaca ttccgccgga
      721 cacgtcctgt aatcccagtt tcccgtccgg caaaacagag aaaatcatgt tcttccgata
      781 tccgtaataa ggatctgatg gaatggttgg ctgaatccat gattcgggat gactcatttt
      841 cccgattctc ttcaactgat cgatcaaaat attccgtttt acttccgttt gatcgaaata
      901 attcagatgt tgcagacgac agtgggcgca agttccgaaa tatctgcatc gtgaagaagt
      961 tcgtttgggg gaataagata aaatttctat taattccgct tgcacgtatc gcttgtccga
     1021 ttttgaatag cgaatcaata cccgctcacc ggggatcgtg ccggggacaa aaacaaccct
     1081 tccgtccgac aatttcccta cagccttacc gtcgctgctg aaatttgtaa ttgttatttc
     1141 ttctttgtta taagcggggc tcaatttttc accgttcttt tcattttttc ctaaattata
     1201 atgggaagtg atgaacgtaa aaaaattgat gcgggcaatc ggttgggaaa ttttcatctt
     1261 gctctgtgga agtcttgccg gctgtcatcc tgtttcaaat caggctatcg gttcggaatc
     1321 ggtacatgtt accttaccgc ctccccaaca aatccagctg ccttccctga ctccgttttc
     1381 cgccgtcgaa atttcgacgg taactttcct cccggaaaat cggtcgaagt cagcgacgtt
     1441 gcctgctttt ttccaaacgc cgacactgaa agttgtttct ggaatcgaac cggattcaaa
     1501 agaatatgac gtcccgcttg tcactcagga tggcctaaaa tataaagggg taaaaactac
     1561 cctcggttgt accgcggcat ccgttcaaat ggtcttggat tattggaaaa aggtaaatcc
     1621 ggattttaag acgatcagtg cccaaaaaat catcgatata aatacttctc agggtacctt
     1681 tcgcaccggt gaagggttat ctattgagaa cgtcaaagac gaattgcaga atatcggcta
     1741 tatccttgaa atttatcaaa acagcgacaa agagcagctt ttaagcgtat tcgatcaatt
     1801 cggcccgcag attttgctgg tcaagaccgg ctgggttccg accggagcca atcacttggt
     1861 tgtcttggtc gcctatgaca aagaaacgga taccgtcacc gtcaatgatc cgtggtttga
     1921 tcatcctttt acttgggact gggaagcttt tgacggaatc tggagtctta actattctca
     1981 aaaagaaaac ggctatctca cccgatcttt tttcaacatc cggcccgcat ctttatccga
     2041 caattcggat taatagacgg cgataatccc gcgattatcg agagttgccc aagatgtgag
     2101 gaaggtatta atcgagcgtg tgtaccgcat tgcaccatcc tgaatcgtca cattttggct
     2161 atcatatccg atgacaacga cggtatgttg atagggaacc actttggtag actttccatt
     2221 ggaaggtgtg taatactgcg gtgtaccgac ttccgtatta ccggctaccc ataccattac
     2281 aggtcgtccg tttgaaattt catattttat tcgttcccaa ctcatgtttt tatatccgac
     2341 tgcgggaacc ccgtagcttc tgaggagcgc cgccacaggt tcttgataga cgccataact
     2401 gtcaggcgga atttgtcccc gtggatctgt gtaattccca acaaatcctt cgtttggatc
     2461 atccgatttc ggcaaactgg ttaaaaattg gtaagcgtcg attgaaaaac caaaataagc
     2521 ggcaaaatca acagccgatc gtgtttcgca atccagcgga taagattggg catgcccgac
     2581 aaatccgtcc acataagctt gggtaggcaa aggctgactg taatcccata tgtcattcaa
     2641 attgccgaaa gagccactga acggcataaa ggtattgtta tcataattgt aattattcgc
     2701 gccctcatct tcggggaacc aaaaccatcc gacataatct tcc
//
LOCUS       DAOMIV010000281         2734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40350_length_2734_cov_3.880552, whole genome shotgun sequence.
ACCESSION   DAOMIV010000281 DAOMIV010000000
VERSION     DAOMIV010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40350_length_2734_cov_3.880552"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /gene="radC"
                     /locus_tag="PKV59_06910"
     CDS             <1..552
                     /gene="radC"
                     /locus_tag="PKV59_06910"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQP46518.1"
                     /translation="NVIDLSKRILNSYGGLGGLKKISFEELKNAKGIGEAKASQVLAA
                     VELGKRLAGLQEENDRLIINSSQDVFDLVSYEMSAFDHEELWVLNVDTRHRLVAIDRL
                     YKGSLNSSTVRISEVFRKPITRNVAAILLVHNHPSGDPKPSAADIAVTKSVQEAGNLL
                     EITMLDHIIIGSGKYESILSYLI"
     gene            630..701
                     /locus_tag="PKV59_06915"
     tRNA            630..701
                     /locus_tag="PKV59_06915"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:662..664,aa:Met,seq:cat)
     gene            715..1374
                     /locus_tag="PKV59_06920"
     CDS             715..1374
                     /locus_tag="PKV59_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46519.1"
                     /translation="MWVHSILQIFGISVATVLAANCFRKFILPTSGNESYPNEPKNEG
                     KIAGFLRTDVIICFVFSWIIYSFLIQNNFLPSAKLSKTAWIILVLIANIDILFYKIPI
                     LLLIVFVLFVIFSGITQKIGINNFLGALFNFLVLYILFVIGKKIYKGKIVPFGFGDVL
                     FGTVLGFALGFINGLGALSFALILSGITAGILKILKKPNFMTIPLVPFFVSGAIFYIL
                     L"
     gene            1386..1901
                     /locus_tag="PKV59_06925"
     CDS             1386..1901
                     /locus_tag="PKV59_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQP46520.1"
                     /translation="MRRADREISEVEEIAKILDSIEIMRIAIFDNPYPYIIPLNFAYF
                     LSHENKITFFFHSANQGRKIELIRQNSHVGFEADKYYGITDSESEIPCKMSTLYESVV
                     GFGRIKIISDNLEKQEGLKILINRFSKVPFSFANYDPILLDRVVILKLDVEGITGKRH
                     MIPNKLSPDIL"
     gene            complement(1953..>2734)
                     /locus_tag="PKV59_06930"
     CDS             complement(1953..>2734)
                     /locus_tag="PKV59_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HQP46521.1"
                     /translation="YAKMIKLSKEHEPLIWNAVHRFGALLENVVYDPATRVIDFDSDA
                     RTENTRGAYPIYFIDGVVENGLGPHPTNIFMLTADATGVLPPLSKLDNNQAMYYFMSG
                     YTSKLAGTERGITEPQPNFSACFGSPFLPLHPSVYAELLGKKIHEHHANVWLLNTGWS
                     GGPYGIGKRFSLPYTRAFVTAALNGEMANVEFVKEPFFGLLIPKTCPGVPSDVLNPRN
                     TWEDKAAYDVAAKKLAAAFIENFKKYEAKTAKEIIEAGPKA"
BASE COUNT          793 a          511 c          572 g          858 t
ORIGIN      
        1 aatgttattg atctttctaa acgaatatta aattcatatg gcggactcgg tggattaaag
       61 aaaatttctt ttgaagaatt aaaaaatgca aaaggaatcg gtgaagcgaa agcctctcag
      121 gttttagctg cggttgaact gggaaaaaga ttagccggat tacaggaaga aaatgataga
      181 ctgattataa attcttcaca agatgtcttt gatttagtca gctatgagat gtcagcattt
      241 gatcatgaag aattatgggt cttaaatgtt gatacacggc atcgattggt tgcgatcgac
      301 agattgtaca aaggatctct aaatagctcg accgtccgga tcagtgaagt cttccgaaag
      361 ccgattacca ggaatgttgc tgctattctt ttggtccata atcatccgag cggtgatccg
      421 aaaccgagcg ccgctgatat tgctgttaca aaaagcgtcc aggaggcggg aaatttactg
      481 gaaataacga tgctggatca tattattatc ggaagcggta aatatgaatc tatcttatcg
      541 tatttgattt aatttatttt atgataccga tttacgagaa aaacggtttg acaaaattta
      601 ctccctatag tattatttca agcgcttacg gcgaggtagc tcagtggcag agcaggggac
      661 tcataagccc tttgtcgtgg gtccaactcc caccctcgcc acttttattt taatatgtgg
      721 gttcattcaa tccttcagat cttcggcatc tccgttgcca cggtgttggc agcaaattgt
      781 tttcggaagt ttattttgcc gacaagcgga aatgaaagtt atccaaacga accgaagaat
      841 gaagggaaaa tagccggttt cttaagaact gacgttataa tctgttttgt tttttcatgg
      901 attatctata gttttctcat acaaaataat tttcttccgt ccgcaaaact atcaaaaaca
      961 gcttggatta ttttggtatt aatcgcaaac atcgacatcc ttttctataa aattccaatt
     1021 ttattgctga ttgtttttgt tctgtttgtg attttttctg gtattactca aaaaatcggg
     1081 attaacaatt ttttgggtgc gttgttcaac tttcttgtct tatatattct gtttgttatc
     1141 ggtaaaaaaa tatataaagg aaaaatcgtc ccgtttggat ttggggatgt acttttcgga
     1201 accgtccttg gctttgcgct gggattcatc aatggtttgg gtgcgctttc gttcgcattg
     1261 attctctccg gaatcaccgc aggaatttta aagattctga aaaaaccgaa ttttatgacg
     1321 attccgttgg tgccattctt tgtttcagga gcgatttttt atattctgtt atagaagagg
     1381 aatttatgag aagagcagat cgggaaatca gcgaggtcga agaaattgct aaaatattgg
     1441 acagtatcga gattatgcgc atagctattt tcgataaccc ttatccatat attattcctc
     1501 tgaacttcgc ctatttttta tctcatgaga acaagattac gttctttttc cattcagcaa
     1561 atcaaggaag aaaaatcgaa ttgattcggc agaattccca cgttggtttc gaagcggata
     1621 agtattacgg aatcaccgat tcagaatcgg agataccttg taaaatgagt acgctttatg
     1681 aaagtgtagt gggatttggc agaatcaaaa taatttccga caatcttgaa aaacaggaag
     1741 gcctgaaaat tcttataaat cgattttcaa aggtgccttt ttctttcgca aattatgatc
     1801 cgattttatt ggatagagtg gtgatattaa aattagacgt cgaaggaatt accgggaagc
     1861 gtcatatgat accaaacaaa ttgagtccgg acattttata aaaaaagcac gaatcgtatc
     1921 gattcgtgct ttttttataa ccaaattccg agttaagcct tcgggcccgc ctcgataatt
     1981 tcttttgctg tctttgcttc gtattttttg aaattttcaa taaaagcagc ggcaagtttc
     2041 tttgcggcaa catcatatgc agccttatct tcccaagtat tgcgaggatt caatacgtcc
     2101 gagggaacac ccgggcaggt cttcggaatc aataatccga agaacggttc tttgacaaat
     2161 tctacatttg ccatttcacc gttcaatgcc gcagtcacaa atgctcgagt gtaaggtaag
     2221 ctgaaacgtt tcccgattcc ataaggaccg ccggaccatc cggtattcag tagccataca
     2281 ttcgcatgat gttcatgaat tttcttgccg agtaattccg cataaaccga aggatgaaga
     2341 ggtaggaacg gtgaaccaaa acatgccgag aagtttggct gcggttcggt aattcctcgt
     2401 tcggttccgg ctaattttga agtgtaaccg gacataaaat aatacattgc ctggttatta
     2461 tcaagtttcg ataatggggg aagtacgcca gttgcatctg cagtcagcat gaagatattc
     2521 gtcgggtgcg gaccgagtcc gttttcaacg accccgtcaa taaaataaat gggataagcg
     2581 cctcgagtgt tttcagttcg cgcgtcgctg tcaaaatcaa tgactctcgt tgccggatca
     2641 tataccacat tttcaagtaa agcaccaaac cgatgaaccg cattccaaat gagcggttca
     2701 tgttccttac tcaatttgat catctttgca tagc
//
LOCUS       DAOMIV010000282         2730 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40434_length_2730_cov_4.680000, whole genome shotgun sequence.
ACCESSION   DAOMIV010000282 DAOMIV010000000
VERSION     DAOMIV010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40434_length_2730_cov_4.680000"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..842)
                     /locus_tag="PKV59_06935"
     CDS             complement(3..842)
                     /locus_tag="PKV59_06935"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQP46522.1"
                     /translation="MEERIQKILSRAGYGSRRDCEVLIKEGRVTRNGEKVELGAKADP
                     DKDQLAVDGVTLKFKEPEKRYIALYKPRFVLSDRSASDPRRTVFSLVPNSDDLFVVGR
                     LDFESEGLVLLTNDGELANKLTHPRYEKEKEYRVLVAKRPDEDQLNKWRRGVVLEDGV
                     KTAPADVRVIRQQGEGAWIRVVMKEGRKREIREIGKQIGLPIVKLVRVRIGSLTLGPL
                     KPGEWVALKDEEVQALKESASAKPAKKPEKKIFRREIAGARMKSGTPALSKGQPKQTK
                     RKS"
     gene            complement(891..1457)
                     /gene="scpB"
                     /locus_tag="PKV59_06940"
     CDS             complement(891..1457)
                     /gene="scpB"
                     /locus_tag="PKV59_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQP46523.1"
                     /translation="MTENSTFDDKEPMTILESILFVASGPVPFSQLAMVTEMKPSEVK
                     ELVRKLAGYLEAENRGIRILVHDDKVQLTTAPETADFVEKFLGIESAQKLTRAAQETL
                     TIIAYKQPVSRPAIDAIRGVNSESVVRGLLSRGLIEEVARAETPGRPVLYATTADFLQ
                     YFGLSSIEELPPFEETIEETDHKQILKD"
     gene            1501..2076
                     /locus_tag="PKV59_06945"
     CDS             1501..2076
                     /locus_tag="PKV59_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46524.1"
                     /translation="MNKHKFILPVIFLLCIFSAGWYAYSPTSQNDSGATALETLETEG
                     NRIANLAAGTPFNLTVSDDTLTSAAEELMRQYKNEIETAIKDAAGVKLSLSNPKVEFT
                     NDGFNLSVKAGLGFLKLKAKINGKVSLVDGKPQVVVEKIDVPIIDISADTVNNEIKSL
                     SDQYMNQLTDSYSLSKIELTEGQAVIEGIKK"
     gene            2147..>2730
                     /locus_tag="PKV59_06950"
     CDS             2147..>2730
                     /locus_tag="PKV59_06950"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HQP46525.1"
                     /translation="MLFFFSFLLIFSTTACQTVDFLNRTGSAEKITVNVEKGGNLLTG
                     TCAAGSTVEQALQELKVKYQPEDIITPVLSTVLSEDTTVKITSVTFEEEQKEQPIPFK
                     SLTVQNESIPEGETYIIQAGQNGSEKITIKSTYEDGVLVSQVTSERNVIRDAVPEILM
                     YGVKAEHAPIQIPGKIVYISNGSAWLLENTTGNR"
BASE COUNT          718 a          710 c          590 g          712 t
ORIGIN      
        1 tgttagcttt ttcgtttcgt ttgtttgggt tgccctttcg acagagcggg ggtgcctgac
       61 ttcatccgag caccggcgat ttctctccga aatatttttt tctccggttt ttttgccggt
      121 tttgcggaag ccgattcttt caatgcctgg acttcttcat ctttcaaggc aacccattcg
      181 cccggtttca gcggacccag cgttaacgat ccgattcgga cccgaaccaa tttaacgatc
      241 ggcaatccga tctgtttccc gatctcccga atctctcgtt tacggccttc tttcataacc
      301 acccgaatcc aagccccttc tccttgctgg cgaatcactc gcacatcggc cggagcggtt
      361 ttgacaccgt cctccaacac aaccccccgc cgccatttgt tcaattgatc ttcatccgga
      421 cgcttggcga ccaatacccg atactctttc tccttttcat aacgaggatg agtaagttta
      481 ttcgccaatt caccatcatt tgtgagtaaa accaaaccct cggattcaaa atccaacctg
      541 ccgacgacaa acagatcatc ggaattcgga accaatgaaa agacagtccg gcggggatcc
      601 gaagccgaac gatcggacag cacaaaacgg ggtttatata aagcgatata tcttttttcc
      661 ggctctttga atttgagcgt taccccgtcc accgccaatt gatccttgtc gggatcggct
      721 ttcgcgccca attccacctt ttcgccattt cgggtaactc tgccctcctt aattaaaact
      781 tcgcaatctc tcctcgaacc ataacctgcc cgtgataata tcttttggat tcgttcttcc
      841 atgtggccct cttctttccc ggatttcccg acaaatgtga aattcctgcc tcaatctttc
      901 aaaatctgtt tgtgatccgt ctcttcgatc gtctcttcaa acggcggcaa ctcttcgatc
      961 gacgataatc cgaagtattg caaaaaatcg gccgtggtgg cataaagaac cggccgcccg
     1021 ggggtttcgg cgcgggcaac ctcctcaatc agtcctcttg agagtaatcc gcgtaccacc
     1081 gactcgctgt tgacccctcg aatcgcatcg atggcggggc gggagaccgg ctgcttgtaa
     1141 gcgataatcg tcaatgtttc ctgtgccgcc cgagtcaatt tttgggcgga ttcgattcca
     1201 aggaactttt ctacaaagtc agccgtctca ggtgcagtgg ttaattgcac tttatcatca
     1261 tgaaccaata tccgtatccc tcgattttca gcctccagat acccagccag tttgcggacc
     1321 aactctttga cttcggatgg tttcatctct gtcaccatag ccagttgcga aaacggtacg
     1381 ggtccggaag ccacaaaaag gatggattcc agaattgtca tcggttcttt gtcatcaaaa
     1441 gtgctgtttt ctgtcatgct ataattctca ccagttcatg aataaaagga gatttaatga
     1501 atgaacaagc ataaatttat tttaccagtc atctttctgc tgtgcatatt ttccgccggt
     1561 tggtatgcat acagtcctac atctcagaat gattcgggag cgaccgcgtt ggaaacgctt
     1621 gaaacggaag ggaatcgcat cgctaatctg gcagcgggta cacctttcaa tttaacggtc
     1681 agcgatgaca ctttaacgag tgccgcggaa gaattgatgc gtcaatataa aaatgaaatc
     1741 gaaacagcaa tcaaagacgc tgcgggcgta aaactgagcc tgtccaaccc aaaagtcgaa
     1801 ttcacaaacg acggatttaa tttgtcggta aaagccggac tcggattcct gaaattaaaa
     1861 gccaaaatca acggaaaagt ctctctggtc gacgggaaac cgcaagtggt ggtcgaaaag
     1921 atcgacgtcc ccatcattga catttccgcg gacacggtca ataatgagat caagagtttg
     1981 tccgaccaat atatgaatca gttaaccgat tcttacagtc tctccaaaat cgagctcaca
     2041 gagggtcagg cagtcattga aggaataaaa aaatagctga agaatgatat caggcggttt
     2101 tttttcaaaa caaaagcaga ttccccgctt tttccgaggc gggaatttgc tttttttctt
     2161 ttcgttcctg ttgatttttt cgacaaccgc ctgtcaaacc gtcgacttcc tcaatcggac
     2221 aggatcagcc gagaaaataa ccgtcaacgt agaaaaggga ggaaatctcc tcaccggaac
     2281 ttgcgctgcc ggctcaacgg ttgagcaggc gcttcaggaa ttaaaggtaa agtatcaacc
     2341 ggaagacatt atcacccccg tactgagcac tgttctcagc gaagatacca ccgtcaaaat
     2401 cacctcggtt acgtttgaag aggaacaaaa agagcaaccg attcccttca aaagcctgac
     2461 cgttcaaaac gaatccatcc ctgaggggga aacttacatt atacaagccg ggcagaacgg
     2521 atcggagaaa ataaccatta aatccaccta tgaagacgga gtgctcgttt cgcaggtgac
     2581 ttcggaaaga aatgtaatcc gtgacgccgt accggagatt ttgatgtacg gtgtgaaagc
     2641 cgaacatgcc cctattcaga ttcccggaaa gattgtttat atctcgaacg gcagcgcttg
     2701 gttgctcgag aatacaaccg gaaaccgcat
//
LOCUS       DAOMIV010000283         2719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40689_length_2719_cov_4.838213, whole genome shotgun sequence.
ACCESSION   DAOMIV010000283 DAOMIV010000000
VERSION     DAOMIV010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40689_length_2719_cov_4.838213"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKV59_06955"
     CDS             <1..313
                     /locus_tag="PKV59_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46526.1"
                     /translation="VAAITHYKLKKGIPTSFQQKTVPLSVKPSGKKVSATKKKRKSLA
                     EISDEAGDQFAKWVEAKIVSIAAKRPNLRSKINELNAADKTDQIQKAIKNFFGTLFRW
                     K"
     gene            351..1070
                     /locus_tag="PKV59_06960"
     CDS             351..1070
                     /locus_tag="PKV59_06960"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQP46527.1"
                     /translation="MAVIEQDLNDTQDFDDEDEIRRLILEAEENQVWLEESGDEIPVE
                     KYENRRFEQKQFMKTEIISSEKQYQGHVFTVSKLNVRLPNGKIRHYDLVEHVPAVTIV
                     PVTNDGKLIFVRQFRMGANQEILELPAGILNGENGDEQPLLAAQRECQEETGYESRTI
                     KRIGGFYMTPGYCTEYIHVYLAQDLVWNPLPQDEDEFLKTELIPIEDAYRMAENGELN
                     DVKTIASLLMAQKDIRRKNEK"
     gene            1277..>2719
                     /locus_tag="PKV59_06965"
     CDS             1277..>2719
                     /locus_tag="PKV59_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQP46528.1"
                     /translation="MARKNITIDANEAVAYVAYRLSEVIAIYPITPSSPIGEHADEWA
                     AKGVKNLWNTVPSIMEMQSEGGAAGAVHGALTTGSLTTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVTARAVASHALSIFGDHSDVMATRQTGFALLASNSVQEAQDMALIAHAAT
                     LKARVPFLHFFDGFRTSHEISKIEQLDEETMKAMIDDELIIAHRNRGLNPEHPFIRGT
                     AQNPDVFFQGREAGNKYYEACPGIVQETMDQFAKLTGRSYHLFDYYGAKDAETVIIAM
                     GSGVETATETAKYLNSKGEKLGVLAVHLYRPFDAATMLKSLPASVKKICVLDRTKEPG
                     SAGEPLYLDVVTAVSEGFTNGTLPFEKMPIIVGGRYGLGSKDFTPAMVKGIFDELSKD
                     KPMNHFSVGIEDDVSGRSIAYDKNFLLPEAKTVRCMFFGLGSDGTVGANKNSIKIIGE
                     ETNNYAQGYFEYDSKKSGSTTTSHLRFGPDP"
BASE COUNT          841 a          550 c          619 g          709 t
ORIGIN      
        1 agtggcagcg attactcatt acaagcttaa gaagggtatt ccgacttctt tccaacagaa
       61 gacagttccg ctctccgtaa aaccgagcgg aaagaaagtt tctgcaacaa aaaagaaacg
      121 aaagagtctc gcagaaatct ctgatgaagc aggagaccaa tttgcaaagt gggtggaagc
      181 aaaaatcgta tcaattgctg ctaaacgtcc caatttacgg tccaaaataa atgaattaaa
      241 cgcagcagat aagaccgatc aaatacaaaa ggccattaaa aatttttttg gtacgctctt
      301 ccggtggaaa taaaattttt taacaatgat caaggaataa ttaattcata atggccgtaa
      361 tcgaacaaga tttgaatgat actcaagatt ttgatgatga agatgaaatc cgccgtctga
      421 ttttagaagc ggaagaaaat caagtctggt tggaagagtc gggagatgag atccctgttg
      481 aaaagtatga aaataggaga tttgaacaga aacaatttat gaaaaccgaa attatcagct
      541 ccgaaaaaca ataccaagga catgttttta cggtttcaaa attaaatgtg cgattgccga
      601 acggaaaaat aagacattat gatttggttg aacatgtccc tgcggtgact atcgtaccgg
      661 taaccaacga cgggaaattg atttttgtgc gccaatttcg aatgggggcg aatcaagaaa
      721 ttcttgagct accggccgga atattgaacg gagaaaacgg agacgagcag ccgcttttgg
      781 ctgcacaacg ggaatgccag gaagaaaccg gatatgaatc ccgtaccata aaacgaatcg
      841 gtggttttta tatgacgcct ggctattgta cagagtatat tcatgtttat cttgctcaag
      901 atttggtgtg gaatccgctg cctcaagatg aagacgaatt tctaaaaaca gagctgattc
      961 cgattgagga tgcctatcgg atggctgaaa atggggaatt gaacgatgtc aagacgattg
     1021 catctttatt aatggctcaa aaagatattc gccggaaaaa tgaaaaataa agcccacttt
     1081 tttattttat ttcactaccg atcccaaaca gaaatgattt aacccctaaa aagagggtac
     1141 aattatagaa ggtccggaaa tgacatccga ttctgttcgg ttgtctgaat gtaggacatg
     1201 ttgctgttga ttaagaatag ggtcaacctt gaatcatacc caaacacaaa taattatatt
     1261 tttaggagaa taatccatgg ctcgaaaaaa cataacaata gatgccaatg aagcggtcgc
     1321 atatgtggct tatcggctca gcgaagtcat tgccatctac ccaataactc catcttcacc
     1381 gatcggtgaa catgctgatg aatgggcagc gaaaggagtc aagaatctct ggaacactgt
     1441 ccccagcatc atggagatgc agtccgaagg cggggcagcc ggggctgtcc acggtgcttt
     1501 aacgaccgga tctttaacga cgactttcac tgcgtctcaa ggcttgttgc tgatgattcc
     1561 gaatatgtat aaaatcgccg gagaactgac ttcaaccgtt ttccatgtta cggcgcgtgc
     1621 ggtcgccagt catgctctat ccattttcgg agatcacagt gacgtaatgg caactcgaca
     1681 aaccggcttt gctcttctgg catctaattc tgttcaggaa gcgcaagata tggcgttgat
     1741 tgctcatgca gcgactttga aagcacgtgt tcctttcctt catttctttg atggcttccg
     1801 aacttctcac gaaatcagca agatcgaaca acttgatgaa gaaaccatga aagcgatgat
     1861 tgatgacgaa ttaattatcg ctcatcgtaa tcgaggttta aatccggagc atcctttcat
     1921 tcgcggcaca gctcaaaatc ccgatgtgtt cttccagggt cgcgaagcgg gaaataagta
     1981 ttatgaagca tgtccgggaa tcgtccaaga gacaatggac caatttgcga agctgaccgg
     2041 tcgttcttat catttgtttg attattacgg tgcaaaagat gccgaaacag taattattgc
     2101 aatgggttcc ggcgttgaaa cggctaccga aaccgcaaaa tatctcaatt caaaaggcga
     2161 aaaactgggt gttctcgctg ttcatctgta tcgacctttt gatgcggcaa ccatgctgaa
     2221 aagtctgccg gcgagtgtta aaaagatttg tgttcttgat cgaacaaaag aaccgggttc
     2281 tgccggtgag cccctctatt tggatgtcgt aaccgctgta tccgaaggct ttacaaatgg
     2341 gactttgccg tttgaaaaga tgccgattat tgtcggcggt cggtacggat tgggctccaa
     2401 agattttact cccgcaatgg ttaagggcat tttcgatgaa ttatccaaag ataaaccgat
     2461 gaatcatttt tccgtcggca tcgaagatga tgtttccggc agaagcatag cttacgataa
     2521 aaacttcttg ctgccggaag ccaaaactgt ccggtgcatg ttcttcggct taggatccga
     2581 tggtaccgtc ggagcgaata aaaactccat taagattatc ggcgaagaaa cgaataatta
     2641 tgcacaagga tatttcgaat atgattccaa gaaatccggt tctactacaa ccagtcacct
     2701 gcgctttggt cctgacccg
//
LOCUS       DAOMIV010000284         2715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40800_length_2715_cov_3.626316, whole genome shotgun sequence.
ACCESSION   DAOMIV010000284 DAOMIV010000000
VERSION     DAOMIV010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40800_length_2715_cov_3.626316"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="PKV59_06970"
     CDS             complement(<1..1421)
                     /locus_tag="PKV59_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQP46529.1"
                     /translation="MNPISFFEKQADSPIWKGIWFLFFFLLPITSMPAVVNLVHSDVV
                     AAPSGLILAVLLIGWVFPKLLSGEKFSENAIPLFCFVIVSLISTAVSFFYEIPIYKQS
                     DPLRSSISAIITVFIGAGFFIAVMLWVQDPKRLKLAMQALNWSGALVLIWTLMQAFFW
                     YTTFGYPQWMKDIQYAVSVGSLYRQRFVGFALEPSWLAHQLNLLYLPFWFAAAVTGYS
                     AHSKRFGKITFERILFVLGAVVLYLTLSRVGLAAFILSILFYFIWRIVHFAETQSAGT
                     KAPKKKKGLILLGFFVLIPFILLFILWTLTKLDFRMAELFTVNIKGRNNPLMYLAEKL
                     SLASRFSYWEGGLKIFSQFPILGVGLGHAGFYLPQNLSAFALRLVEVRDLLFRSEVLL
                     NIKSLWIRILAENGIIGFAFFVAWLVSTWGLSRRMMIRSSSLIKTIGWMGCFSAVAFL
                     LEGFSLDTFALPYLWVSMGLVGGV"
     gene            complement(1428..2204)
                     /locus_tag="PKV59_06975"
     CDS             complement(1428..2204)
                     /locus_tag="PKV59_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46530.1"
                     /translation="MKPFTEHFIKSWHITAVLMISCGLAAFVIAHFLPARYTATAFLS
                     FSIDHNQTGSLTDLEEDRMIGITEDIIKSDEVFAEVCRNMEGCTKESFLNRIQLDRTN
                     NQWYMSVSGEDPQESAAMASVWLDEAYTVLQRCLRAALEERGLKEVLNNLVLCFENSV
                     SGSLTNICPSDTAILFKKIEEISAEIMEKQRLAKGISPAIVLGEKRNVPGDIPVVRSN
                     ATTGFLTLLGLLIGFLISLLLCLMKPGVSKEPKTDCNPVE"
     gene            2393..>2715
                     /locus_tag="PKV59_06980"
     CDS             2393..>2715
                     /locus_tag="PKV59_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46531.1"
                     /translation="MKQLQQNMKNGKIAIKEIPVPEPESGFILIQNAYSLVSAGTERT
                     SADFAEKNLLEKAVSRPDLTKQVIEKAKREGIVSSLESALNRLDIPMYPGYSSAGKII
                     AIGDGV"
BASE COUNT          893 a          662 c          536 g          624 t
ORIGIN      
        1 acacctccga ccaaccccat agaaacccat aaatagggca aagcaaatgt atccaaactg
       61 aatccttcca gcaagaaagc aacggcagaa aagcaaccca tccaaccgat ggttttgatc
      121 agagaactgc ttcgaatcat catccgccgg ctcaatcccc aagtggacac caaccatgcg
      181 acaaaaaagg caaagccgat tattccgttt tccgccaaga ttcggatcca taaactttta
      241 atatttaaga gaacttcgga tcgaaacagt aaatccctga cttcaaccaa acgtaaagca
      301 aaagcgctca aattctgcgg aagataaaat ccggcatgcc cgagccctac tccgagaatc
      361 gggaattgag aaaatatctt caacccgcct tcccaatagg aaaatctgga cgccaaactc
      421 agtttttccg ccaaatacat cagcggatta tttcggcctt ttatgttgac ggtaaataat
      481 tccgccatac gaaaatcgag ttttgtaagc gtccagagga taaaaagcag aataaaagga
      541 attaaaacaa aaaagccgag caggatcagc cctttctttt tcttcggagc ttttgtaccg
      601 gcagattgtg tctccgcaaa atggacgatc cgccatataa aataaaacaa aattgagaga
      661 ataaacgcag ccaaccccac tcgtgaaagc gtcagataaa ggacaaccgc acccaaaaca
      721 aacaatatcc gttcaaaggt aattttcccg aaccgtttgg aatgagcgga atagccggta
      781 acggcagcgg caaaccaaaa aggaaggtat aacagattta attgatgagc taaccatgaa
      841 ggttccagag cgaaacctac aaaccgctgg cggtacagcg aaccgaccga gacggcatat
      901 tgaatgtctt tcatccattg cggataaccg aatgttgtat accaaaaaaa agcttgcatc
      961 agtgtccaaa tcaaaaccaa tgcaccgctc caattcagcg cctgcatagc cagtttcaat
     1021 cgttttgggt cctggaccca caacatcacc gcaataaaaa acccagctcc gataaatacc
     1081 gtaatgatcg ctgaaattga agatcttaaa ggatccgatt gtttatatat gggaatttca
     1141 tagaaaaaag aaacagcggt ggatatcaaa ctgacaatca caaaacaaaa caaaggaatc
     1201 gcattttcgg aaaatttctc tcccgaaagc aatttaggaa atacccatcc gatcaacaaa
     1261 accgccagaa tcaatccgga aggcgcagcc accacatcgg aatgaaccaa attaaccact
     1321 gccggcatgg aagtgatcgg cagcaaaaaa aagaaaagaa accatattcc cttccaaatc
     1381 ggggaatcgg cttgtttttc aaaaaacgat atcggattca ttcgccttca ttccaccgga
     1441 ttgcagtcag tttttggctc tttcgaaact cccggcttca tcaaacacag aagcaaggag
     1501 attaaaaaac caatcaacaa accgagtaat gtcagaaaac ccgttgtcgc attcgagcgc
     1561 acaaccggaa tatctcccgg aacatttctt ttttcgccca atacaatcgc cggagaaatt
     1621 cctttcgcaa gacgctgctt ttccataatc tccgcggata tttcttcgat tttcttgaac
     1681 agtattgccg tatcagacgg gcagatgttc gtcaaagaac cggaaacaga gttttcgaaa
     1741 cagagcacca aattattcaa aacttccttc aatccccgtt cctccaacgc agctctcagg
     1801 cagcgctgca gtacggtata agcctcgtcc aaccagaccg atgccatggc agccgattct
     1861 tgcggatcct ctccggaaac actcatatac cattggttat tggtacgatc caattgaatg
     1921 cgattcagga agctttcttt ggtgcatcct tccatatttc ggcatacctc cgcaaaaact
     1981 tcatccgatt tgatgatatc ttcggtaatt ccgatcatcc gatcttcttc cagatccgtc
     2041 aacgatccgg tctgattatg atcgattgaa aaagagagaa aggcagtggc agtgtacctt
     2101 gcgggaagaa aatgggcgat tacaaaagcg gcaagaccgc atgaaatcat cagcaccgct
     2161 gtaatatgcc aactctttat aaaatgctcc gtaaatggtt tcattgcaat cctattataa
     2221 aaggatttta tgatatcgcg tgaacggctt ccggcacata actggttggt attcatcata
     2281 atttctctta attacccgtt caaaaatgag gattcaaacg caaattccgg aataatgaga
     2341 gtaatgtata attctttcac gagaatcctt tggaatgggg taagagcaaa gaatgaaaca
     2401 gctgcagcag aacatgaaaa acggtaaaat cgccatcaaa gaaattccgg ttcccgaacc
     2461 ggagagcggg tttattctga ttcaaaatgc ctattcctta gtttccgccg gaaccgaacg
     2521 gacctcggcg gatttcgccg aaaaaaacct gcttgaaaag gcggtttcgc gtccggattt
     2581 aaccaaacaa gtgatcgaaa aagccaagcg agaagggatc gtctcctcac ttgaatccgc
     2641 tttgaaccgg ttagatatcc cgatgtaccc cggttattca tccgccggaa agattatcgc
     2701 aatcggcgac ggggt
//
LOCUS       DAOMIV010000285         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_40989_length_2707_cov_4.372926, whole genome shotgun sequence.
ACCESSION   DAOMIV010000285 DAOMIV010000000
VERSION     DAOMIV010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40989_length_2707_cov_4.372926"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKV59_06985"
     CDS             complement(<1..239)
                     /locus_tag="PKV59_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46532.1"
                     /translation="MKQKIRAISLFIIFILFFSSLTAFAQDIESATADIVFTHDMHSY
                     IDPVQVNENGVLVEKGGFARTKTIIDSVKKQNENT"
     gene            complement(291..1331)
                     /locus_tag="PKV59_06990"
     CDS             complement(291..1331)
                     /locus_tag="PKV59_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HQP46533.1"
                     /translation="MIRFNSDYTEGAHPAILERLAETNFVQTNGYGEDEYCFQAKQTI
                     KKVCNKENADVHFLVGGSQTNLTVISSALRPYQSVLSPVSGHIFVHETGAIEATGHKV
                     EALPSTDGKITASQIEEKYLSHIHDESFEHMTQPKMVYISNSTELGTIYNRAELKEIS
                     DVCRQYDLYLYMDGARLGYALTCDENDLDLPFIAECCDVFYIGGTKQGALFGEAVVIT
                     RDELKKDFRYMIKQKGGMLAKGRLLGLQFQVLFEDNLYFQLARHANETAAFLRKSISG
                     AGYSFLIPSPTNQQFPILPNDLLAELQKKYVYSNSQQIDKNHSAVRLCTSWATKWEDV
                     KQLAQDIEGYNE"
     gene            1601..2545
                     /locus_tag="PKV59_06995"
     CDS             1601..2545
                     /locus_tag="PKV59_06995"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQP46534.1"
                     /translation="MKRILVTGACGLVGSDLVLALQSKFGSENIIALDLHDPPKEFQG
                     RFEKMDVRDFEKLKSLILQYDIGQIFHLAGLLSAGSEKNPDMAWDVNLSGLKNVLDIA
                     KDHNIRVFWPSSIGVYGETTPKILTPQHTSFEPNTMYGVTKVSGELLCQYYHHRYGVD
                     VRSLRYPGLNGYKAMPGDGTTEYAIHIFYGLIRENRYECFLSADTRLPMMYMDDAIRG
                     TLMLMDAPSEAITIRTSYNFTAISFTPAELVAEIRKISPDFEIIYKPDYRQKIADSWS
                     QSIDDSQARKDWGWKEEYDLPKMTTALYEGIKERISNE"
BASE COUNT          825 a          508 c          558 g          816 t
ORIGIN      
        1 aaggtatttt cattttgctt tttaactgaa tcaataattg tttttgtgcg agcaaaacca
       61 cccttttcaa cgagtactcc gttctcattc acttgcacgg gatcgatata ggaatgcata
      121 tcatgtgtaa aaacgatatc tgcagtagcc gactctatat cttgtgcgaa agcggtgagg
      181 gaagaaaaga ataagatgaa aataatgaat aacgaaatcg ctctgatttt ctgtttcatt
      241 ttgctctcct ccgaaaaacg attgtgaagt tttaaaataa caattatttc ttactcatta
      301 taaccttcta tatcctgagc gagttgtttc acatcttccc attttgttgc ccaactggtg
      361 caaagacgga cggcactgtg atttttatct atctgttgcg aattcgaata aacatatttt
      421 ttctgtaatt cagcgagaag atcattcggg agaatcggga attgttgatt cgtcggagaa
      481 ggtatcagaa aagaatatcc tgcaccggaa atggattttc ttaagaaagc agccgtttca
      541 tttgcatgac gggctaattg aaaataaaga ttgtcttcaa acaatacctg aaactgtaat
      601 ccgagtaaac gtcctttagc cagcataccg cctttttgct taatcatgta tcggaagtct
      661 ttttttaatt catcacgggt aattacaacc gcttctccaa acagagcgcc ttgtttggtt
      721 ccgccgatat aaaaaacatc acaacattcg gcgataaacg gaagatcgag atcgttttca
      781 tcgcatgtca aggcataacc taacctggct ccatccatat agagatacag atcatattgc
      841 cggcagacat cactgatttc ttttaattcg gcgcggttgt aaatggttcc cagttcggtg
      901 gagttcgaga tatagaccat tttcggctga gtcatatgtt caaaagattc atcatgaata
      961 tggcttaaat atttttcttc gatttgcgaa gccgtaattt ttccgtcggt tgagggaagc
     1021 gcttccactt tatgtccggt ggcttcaatg gctcctgttt catgaacgaa aatatgtccg
     1081 gaaacagggg ataatacgct ctgatacggg cgtaatgcag aagaaataac tgttaaattt
     1141 gtttgggatc ctccgaccaa aaaatgaaca tcggcatttt ccttattaca gactttttta
     1201 attgtctgtt tcgcttgaaa acaatattca tcttcgccat aaccatttgt ctgaacgaaa
     1261 tttgtctcgg caagccgctc gagaatagcg ggatgagcgc cttcggtgta gtcactatta
     1321 aatcgaatca tactgtctat attccttttt tatttacatg gtaacaactg aaatatattc
     1381 tattcttttt cttcgaaaaa atgatcgaaa ccgatttttt tctgagtatg aatcggtgaa
     1441 aataccggat atctacagta caaccggaaa taacacaaaa aacaggatga tcaaatctgt
     1501 tgaaaatcgg ataaaacagg tagaatctta atgttcatga atgcaggttt ggttttaaaa
     1561 accttcgaca gaaggccact ctctttcaca aaggatcaac atgaaacgga tactcgttac
     1621 gggtgcgtgt gggttagttg gcagtgatct ggttctggcg ctacaatcaa aattcggttc
     1681 tgaaaatatt atcgctttgg atttacatga tccccccaaa gaatttcaag ggcgattcga
     1741 gaaaatggat gtgcgagatt tcgaaaagtt gaaatctctg atccttcaat acgatatcgg
     1801 gcaaatattt catttagcgg gattactttc cgccggaagt gaaaaaaatc cggatatggc
     1861 ttgggacgtt aatctgagcg gattgaaaaa tgtcttggat attgcaaagg atcataatat
     1921 cagagttttt tggccgagtt ctatcggtgt atacggcgag acgacgccga aaattctgac
     1981 gccgcagcat accagtttcg aaccgaatac catgtacggt gtcaccaagg tttccggaga
     2041 attactatgt caatattatc atcatcgcta tggagttgat gtacgcagtc ttcgttatcc
     2101 gggtttgaac ggatataagg ctatgccggg agacggaact accgaatatg caatccatat
     2161 cttttatgga ttgattcgag aaaatcgata tgaatgcttt ttatctgccg atacccgttt
     2221 accaatgatg tatatggatg atgcgatccg gggaacttta atgttgatgg acgctccttc
     2281 ggaagcgatt acgattcgta ccagttacaa tttcacggcg atcagcttta cacctgccga
     2341 attggtagct gaaatccgaa aaatctcacc ggatttcgaa attatttaca agccggatta
     2401 ccggcagaag atagcggaca gctggtctca atcgatcgat gattctcaag ctcgaaaaga
     2461 ttggggttgg aaggaagagt atgatctgcc caaaatgacc accgcattat atgaaggaat
     2521 taaagaaagg atatcaaatg agtaaagaaa aattttatga atctttaaaa aatgaagtga
     2581 aacgaataga tgaatccaag acttccaaac gatttgaaaa tttgattgaa gattttaccg
     2641 atgatccgtc tccgcgagcg attattaatg gacaaaagta tgccatattt aattccaatg
     2701 attattt
//
LOCUS       DAOMIV010000286         2706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41021_length_2706_cov_3.450019, whole genome shotgun sequence.
ACCESSION   DAOMIV010000286 DAOMIV010000000
VERSION     DAOMIV010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41021_length_2706_cov_3.450019"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..2331)
                     /locus_tag="PKV59_07000"
     CDS             complement(265..2331)
                     /locus_tag="PKV59_07000"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQP46535.1"
                     /translation="MSNTTGWLIFSLICGIASLILGLYLFFWVKKQDAGDEKAQEIAG
                     WIHGGARAYLKKLYTALIIVAAIMAVILAVVYTLDGSGRGIQTAVCYLIGALCSSMAG
                     YLGMEVAVRANVRSATAASRDLSTAFNVAFRGGAVMGFAVVGIATIGMSAIYLITNQS
                     QLVLAFSLGASSLALLAKAGGGIYTKTADIAADLVGKVEVGIPEDDPRNPAVVADNVG
                     DNVGDVAGMGADIFDSYVASAVAAMLLGAAMGEPHYTILPLLLCALGIVASIIGVAMV
                     RVGNDGKPGKSLNMGTFITCGAFAALMAIAILLVPQIDNNFIWAILVGTAVGVIIGQT
                     TEFFTSDEYSPVKLTAEYSGSGAAITIISGMSYGLISIVPSIIGIVIAMLISFFVCGG
                     MGNFPMGIYGVGISAVGMLAVSGMIVSADAYGPIVDNARGIAEMSGMDEEVIDRSDIL
                     DSAGNTAKAITKGFAIAAAALTVLALFAAFVSEVNLALAPVGKAFNFEDASLLNPLIL
                     AGIFLGAMCPPLFSALTMNSVTKNAFAMIEEIRRQFREEPGIMEGTVRPDYNRCISMA
                     ASGALTSLIVPAIIAIIFPLIVGFIMGPKGLTGFLGGSIITGVIFALFMSNAGGLWDN
                     SKKFIESGQFGGKGSEAHKAAVIGDTVGDPFKDTAGPSLNTLITVMSLVASTFAPVIA
                     LYHLIP"
BASE COUNT          830 a          666 c          563 g          647 t
ORIGIN      
        1 gcgaaaagat agccaaaaaa tactttaagc gataaccgga catcatttgc cagatgcctg
       61 cgatacgatt ctttgttttt attttcgtca agtcaaaatc aattatcggc gttgtcaaaa
      121 taagttacct tgtctcataa tttacacgaa ttaccgcgca gcaaagggta ttatacagga
      181 acattcgaaa aaatgactca ttcccatcaa aaaagcctgc ttgaatcaag caggcttttt
      241 atcaaatcaa gaacaaaaag taatttacgg aatcagatga taaagtgcaa tcaccggagc
      301 aaaagtggaa gctaccaagg acattacggt gatcagagta ttcaatgaag gaccggctgt
      361 atctttgaac ggatcgccga ccgtatcacc gatcacagcc gctttatgag cttccgatcc
      421 tttaccgccg aattgaccgg attcaataaa ctttttggaa ttatcccaca aaccgcctgc
      481 gttcgacata aacagcgcaa agatcacacc cgtaataatg gagccgccca aaaaaccggt
      541 cagccctttc ggacccataa taaatccgac aatcaacggg aaaataatcg caatgatagc
      601 gggaacgatc aaggaggtca acgcaccgct cgcagccata ctgatgcaac gattataatc
      661 gggtctcact gtgccttcca taatgcccgg ctcttcgcgg aattgtcgtc tgatttcctc
      721 aatcatggcg aatgcattct ttgtcacaga gttcattgtc agtgcgctga agagaggcgg
      781 gcacatcgca cccaagaaaa ttccggccag aatcaacgga ttcaacaaag aagcatcttc
      841 gaagttgaag gctttcccga ccggcgcaag cgcaagattc acttcactga caaacgcggc
      901 aaacaacgcc aatacagtca atgcagccgc ggcaatcgcg aatcccttgg taatcgcttt
      961 tgccgtatta cctgcggaat caagaatgtc cgaacgatca atcacttctt catccattcc
     1021 ggacatttca gcaataccgc gagcattatc gacaatcggg ccgtaagcat ctgcggaaac
     1081 aatcataccg gacacggcaa gcatacccac cgccgaaata ccgacaccat aaatacccat
     1141 cgggaagttg cccataccac cgcaaacgaa gaaggaaatc aacattgcta tcacaattcc
     1201 gatgatggat ggaacaatag aaataagtcc gtagctcata ccgctgatga tggtaatcgc
     1261 agcgcctgag cccgaatatt cagcggtcag ttttacgggg ctgtattcat cggaggtaaa
     1321 gaattcggtt gtctgtccga tgatgacacc gactgcggta ccgaccaaaa tcgcccaaat
     1381 gaagttgtta tcgatttgcg ggacaagtaa aatcgcaata gccatcaatg ccgcaaaagc
     1441 accacaagtg atgaaagttc ccatattaag agattttccg ggttttccgt catttccgac
     1501 tctgaccatt gccacaccga taatagaagc aacaattccg agggcacaaa gtaacaatgg
     1561 caaaatcgtg taatgaggtt ctcccatcgc tgcgcccagt aacattgcgg cgacagcgga
     1621 ggcaacataa ctgtcaaaaa tatccgcacc cattccggcg acatcaccga cattgtcacc
     1681 aacattatca gctacgacag ccgggtttcg cggatcatct tccgggatac cgacttccac
     1741 cttaccgacc aggtcggctg caatatccgc agtctttgta taaataccac cgccggcttt
     1801 tgccagcaac gccaaagaag aagcgcctaa agagaatgcc agaaccaatt gtgattgatt
     1861 cgtaatcaaa tagatggcac tcataccgat tgttgcgatt ccgaccacgg caaatcccat
     1921 tactgcgcct ccgcggaatg caacattgaa agcggtagat aaatctcggg atgctgccgt
     1981 cgcggagcgt acatttgctc gaacagcgac ttccataccg agatatccgg ccattgaaga
     2041 gcaaagagca ccgataagat agcagacagc ggtttgaatc cctctgccgc tgccatctaa
     2101 agtgtagacg acagccagaa tcacagccat gatcgcggca acgataatca aagctgtata
     2161 aagttttttg agataagctc ttgctccgcc atgaatccag ccggcaattt cctgcgcttt
     2221 ttcatctccg gcatcctgct tcttcaccca gaagaatagg tataagccga gaatgagtga
     2281 cgcaatacca caaataagac taaagattaa ccaccctgtt gtattactca ttcaaacgct
     2341 cctaaaaaat gatgttatat acggatgcgg aaaagaggtt tccgctatgc tacagtaaaa
     2401 atgttcgaaa aatattcttt tttccccggg aaataaaaaa gaaggctatt tttcagaaca
     2461 ttttaagcga ttatatttta gcacattcgt cggattttgg tagatgtctt ttgtcagaca
     2521 tttcggcgga ttttgttatc cgttttcatc cttcatcttc agaataatcg attcaatgaa
     2581 atcgaatcgg gaaggatttt gaagcgttat aattagccaa ccttaatatt aaggagattc
     2641 atcatggaaa ttcagatttt acgcagtccg tcagaagaag attggatcgg ttgtctgttt
     2701 cttgcg
//
LOCUS       DAOMIV010000287         2705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41040_length_2705_cov_3.924906, whole genome shotgun sequence.
ACCESSION   DAOMIV010000287 DAOMIV010000000
VERSION     DAOMIV010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41040_length_2705_cov_3.924906"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKV59_07005"
     CDS             <1..206
                     /locus_tag="PKV59_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46536.1"
                     /translation="TAKRTELYAKAEDILVKQDAVMIPIYWYTSVQLTKINVERTFSV
                     MGGKESFNKWDILPEGQERTANL"
     gene            537..1592
                     /locus_tag="PKV59_07010"
     CDS             537..1592
                     /locus_tag="PKV59_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46537.1"
                     /translation="MGRYILKRLLYMLLVLFVVSAITFGLMHSVPGGPFDKEKPLPKE
                     IIANLEKRYHLDQPLPVQYLEYVKALIIPEVSTKKFPNTIDDDALFKFKIGNYYVKWV
                     NFGPSYSSRSRTVNDMLKDQLPISIQLGFLAFCVAVIIGMPLGIVAALKQNTFIDYLC
                     MTLAIFGVSVPVIVLGPILIWIFGVSLKWLPVTGWGSKPPFVGGFLPQTWGWQYWKYA
                     IMPMFALGLSSSASIARLTRASLLQVIREDYIRTARAKGLKERAIISGHALKNALIPV
                     VTILGPMFAALLTGTFVTETIFGIPGMGRYFVNSITARDYPVIMGTVLIYAVFLVVCN
                     LIVDLVYGLLDPRIRFE"
     gene            1626..2666
                     /locus_tag="PKV59_07015"
     CDS             1626..2666
                     /locus_tag="PKV59_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46538.1"
                     /translation="MTVDLGNALKNSNNAGNPITLKSESLGKDAFRRLIRNRAALVGG
                     IIIILLYVIMIFADKIAPFDFAQQTLSEQNAVPPWLMKVFPGMQAYGKPPVDNPNKYL
                     LGADYVGRDILSRIIYGSRVSLIIAIIGPLISLVIGIIYGSISGFFGGRVDNIMMRIV
                     DVLYAFPSLLFTILLMTFFRNTLSNPAPGTFGAALSALDSNLGGMLFIFIGIGLTAWE
                     TMARLTRGQVLSIKEKEFVEAARAIGSGNGRIMFRHIMPNIIGPLVVNETLAIPGYIN
                     TEAFLSFIGLGVNPPTPSWGAMISDGSKVVRTYPNQTIFPALMLAITMFAFNFLGDGL
                     RDALDPQMRGRT"
BASE COUNT          702 a          514 c          676 g          813 t
ORIGIN      
        1 acacggcgaa gcggacggaa ctgtatgcca aagcggaaga cattttggtg aagcaggatg
       61 cggtgatgat tccgatctac tggtatacga gtgtacagtt gacgaagatc aatgtggaac
      121 ggactttctc ggtgatgggt ggcaaggaat cctttaacaa atgggatatc cttcccgaag
      181 gccaggaacg aacagcaaat ttataacggt tttaatttgc gagaattcgt tctttatctg
      241 atctgttatc caatgtgatt ttacagtgat caagaggggg ctataattta tagccccctc
      301 aataaatggg aaaattcctc ttttaacgaa gaaataacca tctaagagat gtggaaagag
      361 aaaaaaatca aaataactta ttcaaatcga tatgataatg aaattttcat catcatatcg
      421 acctgaataa ttaaatggat gacaaaaaca atataaaata acaaggaaca ggagttgaca
      481 ggatttatcg atgaactgtt tgatcgaagg tatctgttaa ttccggagga aaagaaatgg
      541 gtagatatat cctgaaaagg cttctttata tgttattggt actttttgtg gtgtcggcca
      601 tcacattcgg tttgatgcat tctgtgccgg gaggaccttt cgataaagaa aagccgttgc
      661 caaaggaaat tattgccaat ttggaaaaac gttatcacct ggatcaacca ttgccggttc
      721 aatatttgga atatgtgaag gcattaatta ttccggaagt aagtacgaaa aaatttccga
      781 acacgattga tgatgatgct ctgtttaaat ttaaaatcgg taactattat gtaaaatggg
      841 tgaatttcgg cccatcctat tcttcacgat cccgaacggt taatgatatg ctcaaagatc
      901 agctcccgat ttcgatccaa ctgggatttt tggctttttg cgtggcggta attattggga
      961 tgcctctcgg gattgtcgcc gcattaaaac aaaatacatt tatcgattat ttatgtatga
     1021 cgttggcgat attcggggta tccgtgccgg tcattgtctt gggaccgatt ttgatttgga
     1081 tttttggggt ttccttgaaa tggttgccgg taaccggttg ggggtcgaag cctccgtttg
     1141 ttggcggatt tttaccgcaa acttggggat ggcagtattg gaaatatgcc attatgccga
     1201 tgttcgcttt gggactctct tcctccgcat ccattgcccg tttgactcgc gccagtttac
     1261 tgcaagtgat ccgcgaggat tatattcgga ccgcccgcgc taaagggttg aaagaacgtg
     1321 ccatcatctc cggacatgcg ttgaaaaatg cattgattcc ggttgtgact attttgggtc
     1381 cgatgtttgc cgcattgttg accggcacgt ttgtcacgga gacaatattc ggcattcccg
     1441 gtatgggacg atattttgta aacagtatca ccgcacggga ttaccctgtg attatgggaa
     1501 ctgtcctgat ctatgccgtg ttcttggttg tttgtaacct gattgtcgat ttggtttatg
     1561 gtttgttgga tccccggatt cgatttgaat gaatttggct gtgagaaaaa tttcaggagg
     1621 aatgaatgac tgttgattta ggaaatgcct tgaaaaattc gaacaatgcc ggcaacccga
     1681 ttacattgaa atcggaatca ctggggaagg atgcttttcg acgattgatt cgaaaccggg
     1741 ccgctttggt cggcggaatc atcatcattt tgctgtatgt gattatgatt tttgccgata
     1801 aaatagcccc gtttgatttt gcccaacaga cgctttcgga gcagaacgcc gtaccgccat
     1861 ggctgatgaa ggtgtttccc ggcatgcagg catacggaaa accgccggtg gataatccga
     1921 ataaatattt gttgggtgcc gattatgtgg gaagagatat tttgagccga attatctacg
     1981 gatcacgggt atcgctgatt attgccatca tcggaccgtt aatcagtttg gtgatcggca
     2041 ttatatacgg cagtatttcg gggttcttcg gcgggagagt tgacaatatt atgatgcgga
     2101 ttgtggatgt tttatatgct tttcccagcc tgctgtttac gatcttgttg atgactttct
     2161 ttcgcaatac tctttcaaat ccggctccgg gaacctttgg agccgccctt tccgctttgg
     2221 acagcaattt agggggaatg ttatttattt ttatcggcat cggactgacg gcttgggaaa
     2281 cgatggctcg tttgactcgt ggtcaggtgc tgtcgattaa agaaaaggaa tttgttgagg
     2341 cggctcgggc gatcggaagc gggaacggac gtattatgtt tcgacatatc atgccgaata
     2401 ttatcggacc gttagttgtg aatgagacgc tggcgattcc gggatatatc aatacggaag
     2461 cattcctttc atttatcggc ttgggagtga atcctcccac tccgtcctgg ggtgcgatga
     2521 tttcggacgg ttccaaagtt gtccggactt acccgaacca aacgatcttt ccggcactca
     2581 tgttggcgat tacgatgttc gcattcaact tcctcggaga cggacttcgg gatgcgctcg
     2641 atccgcaaat gagaggaaga acgtaaagaa ttttgatttc gaagaattat attgccctca
     2701 cccta
//
LOCUS       DAOMIV010000288         2702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41108_length_2702_cov_3.786173, whole genome shotgun sequence.
ACCESSION   DAOMIV010000288 DAOMIV010000000
VERSION     DAOMIV010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41108_length_2702_cov_3.786173"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /locus_tag="PKV59_07020"
     CDS             complement(<1..1235)
                     /locus_tag="PKV59_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46539.1"
                     /translation="MRTFKCPICGSSNEENAKTCAVCLVNFSQLPEDLQLQIQHELEN
                     QQEAVPLQEDEKNDRQNEVPDWLRRRIRPQSDEKENKSFDNYLDLIFHKSVEENQSSA
                     DLNEKSEGDSFPFTQEDFEALEIKEDGVPQETELSPEIEESLSDFSSVRPAQKWDEAS
                     SDNEIVDGKKIVSSDSENEFVDFSKSRPAKKWEDLTPVPNEKIGISGKRETTHPESFE
                     SAKFVSEQKRDKTPLADQKNENIKINSLPEESFVDFSVVRPQQKWDSGQESDAPSSFS
                     VDESSHDVFRDEQLTFLENKSAEPQDVNSELTEEEALYADFSVNRPEKKWDDGNELNS
                     PTADSSSSLETTETVSNKQPFSVTENENSAGKNAVSEEAGLVDSFLRQIAAGTETPQE
                     PVRESVAMTSPEWVTPPEPA"
     gene            complement(1232..1870)
                     /locus_tag="PKV59_07025"
     CDS             complement(1232..1870)
                     /locus_tag="PKV59_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46540.1"
                     /translation="MDLSSKNKRLTVLAGLIGIFFLISAYSDQPAVRTARTMLIHWAS
                     LVAAVLLFIAIIDFVIARIRGIKDHTGSFIYNLISLLIFLIVFFYGFSNQAGASSVQS
                     TVFMIQSSVESVMTGLICLVMIYGIYRVARSQRSLLKSSFLFSTIFFLVLYSGFFSFL
                     KLPAFFSKIIDFIRILPVGGLYGLLIGIAIGALVTGIRVLFFGDHPYQGHKS"
     gene            complement(1884..2633)
                     /locus_tag="PKV59_07030"
     CDS             complement(1884..2633)
                     /locus_tag="PKV59_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46541.1"
                     /translation="MNPSRFGLILIIATLLIYGLCLFLRNKIKLFPLYKNDSNSKKLQ
                     LSLSRSIEEGKPIHLDVLNRDESQLINPSALVAMNTSEQLANRFAASDEPPFVTNNSG
                     LVHALTKDAVKMGYRQADLENEFSATSLDFAGFDSLSHQTTMLGLLEETPYAMHLNIG
                     SGGPEIALQDMMFQNEEEIYIAGDSLLGQATGLATADDVFIGEQLYELPVVTQPERKE
                     AAPDVGLLVMDILRYGFIVALGIGALMILLV"
BASE COUNT          742 a          597 c          592 g          771 t
ORIGIN      
        1 gccggttcgg ggggtgtaac ccattcaggt gaagtcatcg caacggattc tcggacgggt
       61 tcctgcggcg tttccgtacc ggcggctatt tgtcttaaaa atgaatccac cagaccggct
      121 tcctcagaaa ccgcattttt gccggcggag ttctcattct ccgtcaccga aaacggttgc
      181 ttatttgaaa ctgtttccgt tgtttcaagc gaggacgaag aatcagccgt aggagaatta
      241 agttcgtttc cgtcgtccca ttttttttcg gggcgattaa ccgagaaatc ggcataaagg
      301 gcttcttcct ctgtcaattc ggaatttacg tcctgtggct cggcggattt attctcaaga
      361 aaagttaatt gttcatcccg aaaaacatca tgactcgatt cgtcaacgct gaatgaggaa
      421 ggggcatcgc tttcctgtcc cgaatcccat ttttgttgcg gacggactac ggaaaaatcg
      481 acaaaagatt cctccggcag agaattaatt ttgatatttt cgttcttttg atcggccaga
      541 ggtgtcttgt cccttttttg ttccgaaacg aattttgcag attcaaacga ttccggatgc
      601 gtcgtttctc tcttcccgga gataccgatt ttttcgttcg gaaccggcgt caaatcctcc
      661 cactttttag cgggtctgct ttttgaaaaa tcaacgaatt cattttcgga atcggaagat
      721 acgatttttt tgccgtcgac aatttcgtta tcggatgatg cctcatccca tttttgggcg
      781 ggacgaacgg aggaaaagtc cgagagtgat tcctcgattt cgggtgaaag ttctgtctct
      841 tgcggtacac cgtcttcttt gatttccagc gcttcgaaat cttcttgggt gaacggaaaa
      901 gaatcccctt cgcttttctc gtttaaatcc gcggacgatt ggttttcctc tacgctttta
      961 tgaaaaatta aatccaaata attatcaaag gatttatttt ctttttcatc cgattggggt
     1021 cgaatccgtc tccgaagcca gtccggcact tcattttgcc ggtcattttt ttcatcttcc
     1081 tgaagcggaa cggcttcctg ttgattctcc aattcgtgtt gaatttgcaa ttggagatct
     1141 tcgggcaatt gactgaagtt aaccaggcag actgcgcagg ttttcgcatt ttcctcgttt
     1201 gatgaaccgc agatcggaca tttgaaagtt ctcatgattt atgcccttga taggggtgat
     1261 caccgaaaaa caatacccga attcccgtca ccagtgcacc gatggcaata ccgatcaata
     1321 aaccgtaaag cccgccaacg ggcaatattc gaataaaatc tattattttt gaaaagaacg
     1381 ccggtaattt cagaaaagaa aagaatccgc tgtataaaac aaggaaaaag attgtactga
     1441 ataaaaaaga actctttagt aagcttcttt gtgatcgcgc tacccgataa attccgtaaa
     1501 tcattaccaa acaaataagg ccggtcatca cggattcaac cgaggattga atcataaaaa
     1561 cagtcgactg aacggaagat gcgccggcct gattcgaaaa gccgtaaaag aagacaatca
     1621 agaagatcaa taaactgatc aaattgtaga tgaaagatcc cgtatggtct ttgattccgc
     1681 gaatgcgagc gataacaaaa tcgataattg cgataaaaag cagaacggcc gctaccaaac
     1741 ttgcccaatg aatcagcatg gttcgagcgg ttcggacagc cggctggtca gaataggcgg
     1801 aaatcagaaa gaaaataccg atgagccctg caagaaccgt taaccgtttg ttttttgaag
     1861 ataaatccat gaattaatgc cttttatacc aacaagatca ttaaagcgcc gattccgagt
     1921 gcgacaataa agccatatcg caggatatcc atcaccaaca gtccgacatc cggagcagct
     1981 tccttccttt caggctgtgt tacaacgggg agttcgtata gctgttcccc gatgaatacg
     2041 tcatccgctg ttgccagccc ggttgcctga ccgagtaaac tgtctccggc gatataaatt
     2101 tcttcttcgt tttgaaacat catatcttgc aatgcaattt ccggacctcc ggatccgatg
     2161 ttcaagtgca tagcataggg tgtctcttcc agaagaccga gcatcgttgt ttgatgggaa
     2221 agagaatcaa atccggcaaa atcgagggaa gtcgcggaaa attcattttc gagatccgct
     2281 tgtcgatatc ccatttttac cgcatctttt gtaagcgcat gaaccaagcc ggaattattg
     2341 gttacgaacg gcggttcatc ggatgcggca aaacggtttg caagttgttc cgaggtattc
     2401 attgcaacta aagcggatgg gttgatcaat tgtgattcgt cccgattgag aacatccaaa
     2461 tgaatcggct ttccttcttc tatactgcga gaaagactga gctggagttt tttagaattt
     2521 gaatcattct tgtaaagagg gaacaatttt atcttatttc tcagaaataa gcagagtcca
     2581 taaatcagca gggtagcaat gatgagaatc agtccaaacc ttgatggatt catagttttt
     2641 ctcctctcga tcgaacgaat ctgcaattta tgtactgata aaagtatagc atattcgctt
     2701 cg
//
LOCUS       DAOMIV010000289         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41145_length_2701_cov_3.371882, whole genome shotgun sequence.
ACCESSION   DAOMIV010000289 DAOMIV010000000
VERSION     DAOMIV010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41145_length_2701_cov_3.371882"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PKV59_07035"
     CDS             <1..695
                     /locus_tag="PKV59_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46542.1"
                     /translation="YQILVTIHDYMKKELSEVFEKINDYIRNSLMPTFVDLNYLLFYG
                     ENTTYNALGAIFGVTKDINTYMKEQKRIIREELVPAYKYLQENSGLAEGALQAIADAA
                     SGVASQMNSATLATWGLIAALEALNSYQVPGMNPRETGGSSRAKEALAGGGYVSARST
                     YLVGEAGPELFTPSRSGWIIPNDRLAGGGGEQVVYNIEIHDIYGDKYLEQRIKKGVTE
                     GIKNAEFVGLRA"
     gene            667..807
                     /locus_tag="PKV59_07040"
     CDS             667..807
                     /locus_tag="PKV59_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46543.1"
                     /translation="MPSLSDCALSYITSSGEEIILDNYQMERNIWEIYGRTGFDAPAL
                     EY"
     gene            901..1584
                     /locus_tag="PKV59_07045"
     CDS             901..1584
                     /locus_tag="PKV59_07045"
                     /inference="COORDINATES: protein motif:HMM:NF017520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HQP46544.1"
                     /translation="MYGRDMIVRQTVNKLLEHGVRNSWGKLRIKRSDGSFVYLNCHYS
                     GGFDEAARTDPKITLLNLNFHASDPFFYSEDETEFYFSDTEQIGLYFGDEFYFGSRTY
                     FMGGNVTSETIVKNDGQIAYPVITVTGPAKNLKFVNSRNNGRIEIDENFVLAAGETLI
                     IDCRDRMRGITLRDSGGQLTDKTNLLKLGSSLVFPIEQGNNNIEITYTDTSAATSVDF
                     VFQKRWLSA"
     gene            1584..>2701
                     /locus_tag="PKV59_07050"
     CDS             1584..>2701
                     /locus_tag="PKV59_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46545.1"
                     /translation="MAAYEIYQRTVGGMRGDVIDKFTRFTASLAFNDVGKWQLDGAST
                     SGCPLSENDGIIIYRDGDPFLSGYVTDISEEVDETFEGGQIVSWTVQGNDDNGLLTRR
                     ILFPDPVELDITTQAYHTIRDYAGNTILKYIDYHAGEHAASGRRIPNFTIDSYKDLGT
                     IKTWNARFDVLWDFVVNIAATDNLGINVVWDDTIGKYVAFVYRPQDKSDLIIFSREYG
                     NIKKWAHTRSAPKANAIWVAGQGEETERMISYREDADSIAKWGRYEGFKDRRDISNEQ
                     DENDPRTPQEVLDDIAEALIPENKETDTYELELTPINRMSYLDDWNLGDIVKIRLGAI
                     EFNSIINAISIDYQGGVETITPSVGTIDRGTAGKTYST"
BASE COUNT          808 a          550 c          684 g          659 t
ORIGIN      
        1 tataccaaat cctcgtaacc attcatgatt acatgaaaaa ggaactttct gaagttttcg
       61 aaaaaataaa tgactatatt cgaaactcat tgatgccgac atttgttgat ttgaactatt
      121 tgctattcta tggcgaaaat accacatata acgcacttgg tgctattttc ggcgtcacca
      181 aagacattaa cacttatatg aaggaacaga agcggatcat acgtgaggaa ttggttcctg
      241 cgtacaaata tttgcaagaa aactcagggc tcgcagaagg tgctttacag gctattgctg
      301 atgccgcttc cggtgtggct tcgcaaatga actctgcgac cttggcaact tggggattga
      361 tcgcagcttt ggaggcactg aattcctatc aagtacctgg aatgaatccg cgggagaccg
      421 gcggatcaag cagggcgaaa gaagcattag caggtggcgg ctacgttagc gctcgcagta
      481 cgtacctcgt aggcgaagca ggtcccgaat tgttcacgcc gtcacgatca ggatggatca
      541 tcccgaacga ccggttagca ggcggaggag gcgaacaagt ggtttataac atcgaaatcc
      601 atgatattta cggtgataaa tatctggaac aacggatcaa gaaaggtgtg acggagggga
      661 ttaaaaatgc cgagtttgtc ggactgcgcg cttagttaca taacttccag cggcgaagaa
      721 atcattctgg ataactacca gatggaaaga aacatctggg aaatctacgg aagaaccggc
      781 ttcgatgcgc cggcgctgga atattaagat gaaatttatg ctgacgggta ttctgagact
      841 ttagcggtca gaattccccc tcgggatata gcgatcaacc ttgtcatccg cggatctact
      901 atgtatggtc gcgatatgat cgttcggcag accgtaaata agcttttgga acatggcgtt
      961 agaaattcat ggggtaagtt gcggataaag cggtcggacg ggtctttcgt atatctaaat
     1021 tgccattaca gcggcggttt tgacgaggca gcaaggactg accccaaaat aacgctgcta
     1081 aaccttaact ttcatgccag cgatccattt ttctatagtg aggatgaaac agagttctat
     1141 ttttccgaca cagaacaaat tgggctctat tttggagatg aattttactt tggcagcaga
     1201 acttatttca tgggcggaaa cgtaaccagc gagacgatcg tcaaaaatga tgggcagatc
     1261 gcttatccgg taattaccgt aacaggtccg gctaaaaact tgaagtttgt gaacagcagg
     1321 aataacggac gaatagagat tgatgaaaac tttgtccttg ccgccggaga aacgctgatc
     1381 attgactgcc gtgaccgtat gcgagggata acgctcagag acagcggagg gcaattaaca
     1441 gataaaacga accttctgaa gctcggatca tccttggttt ttccgatcga gcaaggtaat
     1501 aataacattg aaatcacgta tacagatact tcggcggcaa cgagcgttga ttttgttttc
     1561 cagaaaaggt ggctaagtgc ataatggcag cttatgagat ttaccaaagg acagtaggcg
     1621 ggatgagagg cgacgtaata gacaaattta ctcgatttac tgcttcactc gcttttaatg
     1681 acgtcggcaa atggcaattg gacggcgcat cgacttcagg ttgtccgctg tcagagaatg
     1741 acggcatcat catatacagg gacggcgacc catttctaag cggttatgta acggatattt
     1801 ccgaagaagt cgacgaaact ttcgaaggtg ggcaaatagt atcatggaca gtacagggta
     1861 atgatgataa cggattgcta accagaagga tattattccc cgatccggtg gagctggata
     1921 tcacaacaca ggcatatcat acgattcgcg actatgccgg caatacaatt ttgaaatata
     1981 tcgattatca tgccggagaa catgcagcaa gcggcagacg aataccgaat tttacgattg
     2041 attcatacaa agacttgggc acgatcaaaa catggaatgc tcgttttgac gtactttggg
     2101 acttcgtcgt aaatatagcg gcaacggata atctcggcat taacgtggtt tgggatgata
     2161 cgatcggtaa atatgttgct ttcgtttatc gtccgcaaga caaaagtgat cttattattt
     2221 ttagccgaga gtacgggaac atcaaaaaat gggcgcacac gcgatcggcg ccgaaagcga
     2281 atgctatttg ggtcgcaggg caaggcgaag aaaccgagcg gatgatcagt tatcgggagg
     2341 atgcagattc tatcgcaaaa tgggggcgct atgaaggatt caaagatcgg cgagatattt
     2401 ccaatgagca ggatgaaaat gacccgagga cacctcagga agttttggac gatatcgcag
     2461 aagcgcttat accggagaac aaagaaaccg acacctacga actggaatta acgccgataa
     2521 accgtatgtc ttatcttgat gattggaacc tcggcgatat tgtcaagatt cggcttggcg
     2581 ctatagaatt taactcgatc atcaacgcaa tttccattga ctatcaaggc ggagtcgaaa
     2641 cgatcacgcc gtctgtcggc acgattgacc ggggaacagc cggtaaaacc tactcaacga
     2701 t
//
LOCUS       DAOMIV010000290         2691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41369_length_2691_cov_3.267830, whole genome shotgun sequence.
ACCESSION   DAOMIV010000290 DAOMIV010000000
VERSION     DAOMIV010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41369_length_2691_cov_3.267830"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1021)
                     /locus_tag="PKV59_07055"
     CDS             complement(77..1021)
                     /locus_tag="PKV59_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HQP46546.1"
                     /translation="MLKEKRITITGAKGFLGGHLLKKFVSSGCRNISIADLPDYNLTD
                     INDIRKMYDETKPEIVIHLAAKVGGIGFNQNKPAELFYDNLMMGSQLLHEAYLRNVEK
                     FVALGTVCAYPKFTPVPFKEDDIWNGYPEETNAPYGLAKKMMLVQSQAYRKQYGFNSI
                     FLLPVNLYGPGDNFDPDSSHVIPALIKKCFDALDSKSDILEVWGTGAATREFFYVEDA
                     AEAIYLATLSYNKSEPVNLGAGFEISIRELIKIIADLAGFSGQIVWDKSKPDGQPKRM
                     LDTSKASIEFGFTAKTDIHTGLAKTIEWYRENRHLLKK"
     gene            complement(1037..2062)
                     /gene="gmd"
                     /locus_tag="PKV59_07060"
     CDS             complement(1037..2062)
                     /gene="gmd"
                     /locus_tag="PKV59_07060"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQP46547.1"
                     /translation="MKKAFLTGITGQDGSYLAEFLLNKGYEVHGLIRRSSTFNTERID
                     HLYHDFHDPDAKLFLHYGDLSVSAQLMDLLHSIQPDEIYNLGAQSHVRVSFDMPEYTG
                     DVTALGTLRILEAIRKTGIKTKFYQASSSEMFGAAAPPQNENTVFQPRSPYAAAKVYS
                     YYITQNYRDAYGMFASNGILFNHESPRRGETFVTRKITRAATQIKLGLKDKLYLGNLE
                     AKRDWGFAGDYVEAMWLMLQQEKSDDFVIATGETHSVREFAEKVFNKMNLDYKKFVKI
                     DQRYFRPTEVDALLGDSTKAKIILGWQPKVSFEKLIDMMIEADMELAQKEKTLLDAGF
                     SSNNKNK"
     gene            complement(2133..>2691)
                     /locus_tag="PKV59_07065"
     CDS             complement(2133..>2691)
                     /locus_tag="PKV59_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQP46548.1"
                     /translation="GFRFTFGGYQNLTGIKPDITCLGKIIGGGLPVGAVGGKKEIMER
                     LAPRGDIYQAGTLSGNPLAMIAGLTTLNILKTRNNSFLSLGKKTDKLCCEIEKLFARK
                     GIAVCINRLYSMFTIFFTKGPVFDLNSAQKSDKKMFARYFHGMLKNGIWLAPSPYEAA
                     FLSFAHTNRDFEKTIDACSRTLKHL"
BASE COUNT          773 a          578 c          519 g          821 t
ORIGIN      
        1 ttcgggtctc atcatccact gccgaatgct ccttaacatg aatgaaactg caagtcaata
       61 aaaatatgac cggcgttcat ttttttaata aatgacggtt ttctctatac cattcaatag
      121 tttttgccaa tccggtatgt atatccgttt tagccgtaaa tccaaattct attgatgctt
      181 ttgaagtgtc cagcatccgt ttgggctgtc catcgggttt ggatttatcc cagacaatct
      241 gaccggaaaa accagctaaa tcggcaataa tttttatgag ctctctaatg gatatttcaa
      301 atccggcccc gagattaacc ggctcacttt tattgtaaga taatgttgca agataaatag
      361 cttcggctgc atcttccacg taaaaaaatt cacgggtggc agccccggtg ccccatactt
      421 ccaatatatc agacttactg tccaatgcat caaagcattt ctttatcaga gcggggataa
      481 cgtgggaaga atcgggatca aaattatctc ccggaccgta aagatttacc ggcaataaaa
      541 atatagaatt aaaaccgtac tgtttacgat aagcctgaga ttgaaccagc atcatttttt
      601 tcgccaaacc ataaggggca ttggtttcct caggatatcc gttccagata tcatcttctt
      661 taaacggtac gggagtaaat ttgggataag cgcaaaccgt tcccaaagcg acaaattttt
      721 caacatttcg gagataggct tcatgaagca attgtgaacc catcatcaaa ttatcataga
      781 aaagttcagc cggtttgttc tgattaaaac caattccgcc aactttggcg gcgagatgaa
      841 taacgatttc cggttttgtt tcgtcataca ttttccggat atcattaata tccgttaaat
      901 tataatccgg aagatcagca atgctgatat ttcggcaacc ggaactgacg aattttttca
      961 aaagatgacc gccaagaaaa cctttagcac ctgtaatcgt tatgcgcttt tccttcagca
     1021 cgatggaatc ctttttttac ttgtttttgt tattagagct gaaaccggca tcaagaagag
     1081 ttttttcttt ttgagccagt tccatgtccg cttcaatcat catatcgatc agcttttcaa
     1141 atgaaacctt cggttgccag ccaagaatta tttttgcttt tgtcgaatca cccaaaaggg
     1201 catcaacttc agtgggacgg aaatatcgct gatctatttt tacaaatttt ttataatcca
     1261 gattcatctt gttgaatacc ttttccgcaa attctctgac cgagtgagtc tctcctgtcg
     1321 cgatgacaaa atcatcagat ttttcctgtt gcagcatcag ccacatcgct tcgacataat
     1381 caccggcaaa tccccagtct cttttggcct caagatttcc taaatacagt ttatctttca
     1441 aacccagttt tatctgtgtt gccgcacgag taatttttct cgtcacgaaa gtttcaccgc
     1501 gtctcggaga ttcatgatta aaaagaattc cgttagaagc aaacatccca taagcgtcac
     1561 ggtaattttg agtaatatag taagaatata ctttggctgc cgcgtaagga ctgcgaggtt
     1621 gaaaaacagt attttcattt tgcggcggag ctgctgcgcc gaacatttcg ctggatgacg
     1681 cctgataaaa tttggttttg atgcctgttt tacgaatggc ttccaggatt ctcaaggttc
     1741 ccaatgcggt gacatcaccg gtatattccg gcatatcaaa actgacacgc acgtgacttt
     1801 gcgcacccag attatatatt tcatcgggtt gaatggaatg aagtaaatcc atcaactgag
     1861 ccgaaacact taaatctcca taatgaagaa ataatttagc atcaggatcg tgaaaatcgt
     1921 gatagaggtg gtcaatacgt tcggtattaa acgtactcga acgtctgatt aatccgtgaa
     1981 cttcgtaccc tttattcagt agaaattcag ctaaataaga accatcttgt ccggtaatgc
     2041 ccgtcagaaa ggcttttttc atgtgatatc tccatgtaaa gtaaatattg cttattcaat
     2101 acaggattga ttgaggcttg tcaatgtgcc actcaaagat gctttaatgt tcgcgaacac
     2161 gcatcaatgg ttttctcaaa atctctattg gtatgagcaa acgacaggaa cgctgcttca
     2221 tatggtgagg gcgcaagcca tataccattt ttaagcatgc cgtggaaata cctggcaaac
     2281 attttcttgt ctgatttctg agcgctattt aaatcaaata caggaccttt ggtaaaaaaa
     2341 atggtgaaca tggaatatag gcgatttatg caaacggcaa ttcccttccg ggcaaacaat
     2401 ttttctattt cacagcaaag cttatcggtt ttcttaccaa gagacaaaaa ggaattattt
     2461 cttgttttaa ggatattgag tgtagtgagg ccagcaatca ttgccagagg gtttcccgac
     2521 agggttcccg cttgatagat atctccgcgc ggtgcgagtc tttccatgat ttctttttta
     2581 ccaccgacgg ctcctactgg cagacctccg ccgataattt tgcccagaca ggttatatcg
     2641 ggcttgatgc ctgttaaatt ctgatatccg ccaaatgtaa aacgaaagcc g
//
LOCUS       DAOMIV010000291         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41488_length_2685_cov_4.541065, whole genome shotgun sequence.
ACCESSION   DAOMIV010000291 DAOMIV010000000
VERSION     DAOMIV010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41488_length_2685_cov_4.541065"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>2685
                     /locus_tag="PKV59_07070"
     CDS             68..>2685
                     /locus_tag="PKV59_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HQP46549.1"
                     /translation="MDKQKMVAVVGLGAILPDALDVPSFWNNIVSNKYSITEVPSDRW
                     NVDLYYDPDPAAADKTYSKIGAFVKGYRFDSLKNGIAIPPKVLEVMDITQQWAISASR
                     QALMDYGYPERKLNPERVAVIFGNADGGERHYRSTMRILLPETLEFLRSVPDFAQLPE
                     DIRSAIEKGLATNIHSSIPATSEDTLPGEISNIIAGRVANVFNFNGQNFVTDAACASS
                     MAALQAAAEGLIAHRFDAVLTGGIDRNMGPESYVKFSKIGALSADGSRPYAEGANGFV
                     MGEGAAVFLLKRLEDAERDGDKIYAVVCGIGSSSDGKGKGITAPNPVGQKLAIERAWQ
                     DAGIFPESVGLVEGHGTSTKVGDVAEVNSLNAIFGSSGLKNGSVVLGSVKSNIGHLKS
                     ASGAAGLLKTILSLHYRVLPPSANFTKPNPNIDFNNLPFRVNKQAEEWTVKEGNHRYA
                     GVSSFGFGGTNFHIVLEEYYQGIGGTSSSTYVIPIDIHSSTGNSTASFDRSNSEIKQE
                     AMKVPTESQEIDIDEIKRYVLTTVSEKTGYPVEMLDPDLDLEADLGIDTVKQAELFAT
                     IRNNYGISRQEDLILSNYNTISKLIDFIQESITSLPAQNTPSLTEQKEEAEIKTTLEN
                     MSSETAELKPYQGLFFASADSAAELAADLRQKLTDLRAGMPPESHIPSEAERSHAERI
                     AIDYADIEELPAKLEKALTALNTGTAGAWKSAQAYGIYHGSGKPGKVAFLFPGQGSQY
                     VNMLRDLWDSEPIVRDTFLEADRIMTPILGRPLTAYIYTDGGEQEIKQAENELKNTAI
                     TQPAVLTANVAILRILQKYGIEPDLVMGHSLGEYAALVTAEVLTFAEALEVVCARGQE
                     MSKIKVDDPGCMAAVSA"
BASE COUNT          757 a          609 c          677 g          642 t
ORIGIN      
        1 aaaatccgat tcatagacgt gttcaatatt tgcaatcaaa attcagcatc aataaggaga
       61 tttaagcatg gataagcaaa aaatggtcgc ggtagtaggt ctgggagcga tcttacctga
      121 tgcattggat gttccttcgt tttggaataa cattgtttca aacaaatatt caatcaccga
      181 ggttccatcg gaccgatgga atgtggatct ctattacgat cccgatcctg ccgcagcaga
      241 caaaacttac agcaaaatcg gcgcgtttgt caaaggatac cggtttgatt ctctcaagaa
      301 cgggatcgcc attccgccga aagtgctgga agtaatggat attactcaac aatgggctat
      361 ttcggccagt cgacaagctc ttatggatta cggatatccg gaaagaaaac tgaatccgga
      421 acgtgtcgcc gtcattttcg gcaacgcaga cggcggcgaa aggcattatc ggtcaaccat
      481 gagaattctg ttgccggaaa cgttggaatt tctccgatcg gttccggatt ttgcacagtt
      541 gccggaagat attcgatcag ccattgaaaa aggcctggcg acaaacattc attcttccat
      601 tccggcaacc tcggaagata ctttaccggg ggagatttca aacattatcg ccggacgggt
      661 tgccaatgtc ttcaatttca acgggcagaa ttttgtaacc gatgcggctt gtgcttcctc
      721 aatggcagcg cttcaggctg ctgccgaggg attaatcgct catagattcg atgccgttct
      781 gacaggcggt atcgatcgaa atatgggacc ggaatcctac gttaaattca gtaagatagg
      841 cgctttgagt gcggacggtt cccgccccta tgccgaggga gccaacggtt ttgtcatggg
      901 tgaaggcgcg gcagtcttcc tattaaaaag attggaggat gccgaacggg acggcgataa
      961 aatttatgcg gttgtttgcg gcatcggcag cagttcagac ggcaagggaa aagggatcac
     1021 cgcacccaat cctgtcgggc aaaagctggc aatcgaacga gcttggcagg atgcgggaat
     1081 attcccggaa tccgtcggtt tggttgaagg ccacgggaca tccacgaaag tgggggatgt
     1141 ggctgaggtt aatagcctga acgcgatttt cggttcaagc gggctgaaaa acggcagcgt
     1201 agttctcggt tcggtaaaat caaatatcgg acatctgaaa agtgcatccg gtgcagccgg
     1261 tctgttaaaa accatccttt ctcttcatta tcgggtactt ccgccttcgg caaatttcac
     1321 taaaccgaat cccaacattg atttcaacaa tctgcccttc agagtgaaca aacaagccga
     1381 agaatggacg gtgaaagagg gaaatcaccg ctatgcggga gtcagctcat tcggtttcgg
     1441 cggaacaaat ttccatattg tattagaaga atactatcag gggatcggag gcacgtcgag
     1501 ctcgacttat gtcatcccga ttgatatcca ttcctcaaca ggaaattcga cggcatcatt
     1561 tgaccggtca aattcagaaa taaaacagga agccatgaaa gttccgacag aatctcaaga
     1621 aatcgatatc gatgaaatca aaaggtatgt tctgacgaca gtcagcgaaa aaacgggata
     1681 ccccgttgaa atgttggatc cggacctgga tctggaagcg gatttaggca tcgataccgt
     1741 aaaacaagcc gagctttttg caacgattcg aaataattac ggcatttccc gccaggaaga
     1801 ccttatttta tccaattaca acacgatctc aaaattgata gattttattc aggaatctat
     1861 tacttctctc cccgctcaaa ataccccgtc gttgacggag caaaaagaag aagctgaaat
     1921 aaaaaccaca ctcgaaaata tgtcttctga aaccgcggaa ttgaagcctt atcagggttt
     1981 gttttttgct tcagcggatt ccgccgctga attagcagct gacttaagac aaaaattaac
     2041 agatcttcga gcgggaatgc cgccggaatc ccacattccg tcagaagcgg aacgatcgca
     2101 tgcggaacgg attgccatcg attatgcgga tatcgaagag ctccccgcaa agttggagaa
     2161 agctttaact gctttgaata ccggtactgc cggtgcttgg aaatccgctc aggcttacgg
     2221 catttatcac ggaagcggga aacccggaaa agtggctttt ctcttcccgg gacagggctc
     2281 gcaatatgtc aatatgttgc gagatttatg ggactccgaa cccatcgttc gcgatacgtt
     2341 ccttgaagcg gatcgaatca tgaccccgat cttgggacgt ccgttgaccg cctatatcta
     2401 taccgacggt ggcgaacagg aaatcaaaca ggctgaaaat gaattaaaaa ataccgcgat
     2461 tacccagccc gcagtactca cggcaaacgt cgcgatttta cgaattttac agaaatacgg
     2521 catcgagccg gatttggtca tgggtcacag tttgggcgaa tatgcggcat tagtgacagc
     2581 cgaagtcctg acctttgccg aggcgttgga agtggtatgc gcacgcggac aggaaatgag
     2641 caaaatcaag gtggatgatc cggggtgtat ggcagccgtt tcggc
//
LOCUS       DAOMIV010000292         2682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41556_length_2682_cov_3.565664, whole genome shotgun sequence.
ACCESSION   DAOMIV010000292 DAOMIV010000000
VERSION     DAOMIV010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41556_length_2682_cov_3.565664"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..370)
                     /locus_tag="PKV59_07075"
     CDS             complement(152..370)
                     /locus_tag="PKV59_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46550.1"
                     /translation="MKRNPFFLFLAIIIILSMTLTACQSSTETKPTPVFEFKQFNPTK
                     APVHPTVPSTVTSNPNNTVETEATKVPD"
     gene            complement(505..1872)
                     /locus_tag="PKV59_07080"
     CDS             complement(505..1872)
                     /locus_tag="PKV59_07080"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQP46551.1"
                     /translation="MRWDREQTYELLKRIIDLSEADQTEACFMAYESGLTRFAENRIH
                     QNMCEEDAIVVIRVAIGKKLGSVSANQFDEASLVCAIQKAIEIASLNQPNELFDGFAE
                     KQPVDSIRLPWYIPETASAGTNERADIASRLIQKVENSAYEAAGSVSNGVYVSSVLTS
                     LGQFVYQQETRCGGLTVVNRPGEAGFGTGYSEYFGRDIHLIHPEELAAKAVCISAMNH
                     DAKSLPPGDYPAVLTPASVGLLIFYLGWMALHARAVQSGQSFLAGYFGEEVLDPKLSI
                     WDDALDGRNYGMSFDWEGNPARYVDLIRNGKAIDVVYDTFTAKKVGKKSTGHALSPLR
                     DRYYSSPMAEHLIVGTGDTPLEKMIEKTEKGILINHLWYVREVNFKSASVTGMTRDGT
                     FMIENGKITHALKNLRFSISLRDVFQSIEMIGTEQELTPVSIGTVLAPAMKLKHFNID
                     GSSSY"
     gene            complement(1877..>2682)
                     /locus_tag="PKV59_07085"
     CDS             complement(1877..>2682)
                     /locus_tag="PKV59_07085"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQP46552.1"
                     /translation="EGSKRFAQQAVELLSAKRCKSGKKTIILDSSMLTLMVHESIGHA
                     LESDRVLGSEASYAGTSFATPEKLADYHYGSEQVTITSDPTLPGGLASYGYDDEGYPA
                     CRTVLIDHGILVGYLTGRETAAKLKLPLTAAMRSDGWQRFPLIRITNVNLEPGDSSLA
                     KMISEVDDGFYMETVKTYSIDDKRLNFQFAPEIAWEIKNGKIGSMVKNVRYSGMTPEF
                     WNSCDAVANRDEWHLWGIPVCGKGEPGQVVMVGHGTAPARFQNVSVLGD"
BASE COUNT          728 a          642 c          499 g          813 t
ORIGIN      
        1 gttaattccc gacgatgaca tatcaaagtc cgggttatgc gatttgtttt ggtctagaaa
       61 cttttattct atttttttac attaaactaa tttataaaaa ttttcttgtg atcggttaat
      121 aaaaaaaatc ttcatcactc cttcctggta tttaatcggg tacctttgtc gcttctgttt
      181 caaccgtatt atttggattg cttgtaacgg tagagggaac agtcggatga acaggtgcct
      241 tcgtcggatt gaattgttta aactcaaaga caggggttgg tttcgtttct gtcgatgatt
      301 gacatgccgt caaagtcatg cttaaaataa tgattatggc aaggaaaagg aaaaatggat
      361 ttctcttcat aatatgtgct cctaaactat tgtttcaaca aacgacaaaa aggaaattta
      421 tttttcttat tataccgata ttttgtcttt ttcagataaa aatcaatctc tcttttaaaa
      481 ataattacaa ctcattttcc tcgatcaata cgaggaggaa ccgtcaatat taaagtgctt
      541 taatttcatt gccggagcga ggacggtccc tatggatacc ggggtcaatt cttgttcagt
      601 cccaatcatt tcgattgatt gaaatacatc tctcagactg atcgaaaaac gtaaattttt
      661 taacgcatga gtgattttgc cgttttcgat cataaaagtt ccgtctcttg tcattcccgt
      721 taccgatgca cttttaaaat tcacctcacg cacataccat aaatgattga tcaatattcc
      781 tttttctgtc ttttcaatca ttttttctaa aggggtatct ccggtcccga cgattaaatg
      841 ttctgccatc gggcttgaat aataacgatc tcttaaagga cttaaggcat gtccggttga
      901 ttttttcccg acttttttcg ctgtaaaagt atcatagacg acatcgattg cttttccgtt
      961 ccgaatcaga tcaacatacc gcgccggatt cccttcccaa tcaaaactca ttccgtaatt
     1021 tcgtccgtct aaagcatcgt cccaaatgga tagtttaggg tctaatacct cttcgccaaa
     1081 ataccccgcg agaaaagatt gaccggattg aaccgcccgt gcatgcaaag ccatccaccc
     1141 cagataaaaa atcaacagac cgaccgaggc gggagtcaat acagccggat aatctcccgg
     1201 gggcaaactt tttgcatcat gattcattgc cgaaatacaa acagcttttg ccgccaattc
     1261 ttcggggtgg atgagatgaa tatcgcgtcc aaaatattcg gaatatccgg ttccgaaccc
     1321 cgcttctccc ggcctgttaa ccactgtcaa ccccccgcag cgtgtttctt gttgatagac
     1381 aaattggccg agacttgtta aaaccgatga aacatagaca ccgtttgaca ccgatcctgc
     1441 agcctcatat gccgaatttt caaccttttg aattaaccga gaagcaatat ccgcccgttc
     1501 attcgttcct gccgaagctg tttcgggaat ataccagggc aaacgaatac tatcgaccgg
     1561 ttgtttttct gcaaaaccgt caaataattc attgggctga ttcaacgatg caatttcaat
     1621 tgccttttga atggcacaaa cgagagatgc ttcatcaaat tggtttgcgg atactgaacc
     1681 gagttttttc ccaatggcaa cccgaataac cacaatcgcg tcttcttcac acatattctg
     1741 atgaattctg ttttccgcaa atctggtcaa tccggattca tatgccatga aacaggcttc
     1801 ggtttgatcg gcttcggata aatcgattat tctcttgagc aattcataag tctgttctct
     1861 atcccatcgc atgcgttcaa tctcccaaaa cagacacatt ttgaaatcga gccggagctg
     1921 ttccgtgccc aaccatcaca acctgacccg gctcaccttt cccgcaaacc ggaattcccc
     1981 ataaatgcca ttcatctcga tttgcaaccg cgtcacagga attccaaaat tccggtgtca
     2041 ttccgctata tcgtacgttt ttcaccatcg aaccgatttt cccgttttta atttcccagg
     2101 caatttcagg cgcaaattga aaatttagcc gtttatcatc aatgctgtac gtttttacgg
     2161 tttccatgta gaaaccgtca tcgacttccg aaatcatttt cgccaatgaa gaatcccccg
     2221 gttctaaatt gacatttgtg atgcgaatga gcggaaacct ttgccaaccg tctgagcgca
     2281 tcgcagcagt aaggggtaat tttaattttg ccgcggtctc ccgaccggta agataaccga
     2341 ccaatattcc gtgatcgatc aataccgtcc ggcatgccgg ataaccttcg tcatcatatc
     2401 cataactcgc aagtccgccc ggaagtgtcg ggtcggaagt gattgttacc tgttccgatc
     2461 cgtaatgata atcagccagt ttttcgggtg tagcaaaact tgttccggca taactggcct
     2521 cactgcctaa gacacgatcc gattccaatg cgtggccgat gctctcatgc accattaatg
     2581 tcagcataga actgtccaaa atgatggtct ttttccccga tttacatcgt ttcgccgata
     2641 aaagttcgac agcttgttgg gcaaatcttt tcgaaccttc ga
//
LOCUS       DAOMIV010000293         2679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41627_length_2679_cov_4.270960, whole genome shotgun sequence.
ACCESSION   DAOMIV010000293 DAOMIV010000000
VERSION     DAOMIV010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41627_length_2679_cov_4.270960"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PKV59_07090"
     CDS             complement(<1..993)
                     /locus_tag="PKV59_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQP46553.1"
                     /translation="MIDAHHPSLTSVYAADPARYDNMIYRRCGKSGVQLPILTLGLWH
                     NFDGYKSFETDRAMIRYAFDHGITHFDIANNYGPPYGAAERVFGQIYEQDLKPYRDEL
                     FISTKAGYDMWPGPYGDYGSKKYLVASLNQSLKRMGLDYVDVFYHHRPDPETPLEETM
                     GALDFLVRQGKALYVGISNYPADLTKKAAEILRELGTPCLIHQPSYSILNRWTETDHL
                     FDITAEYGMGTIVFAPLQHGLLSSRYLNGIPEGSRASLDRHLKKDLITDESIGYIRQL
                     NSIAVERGQTLSQMALAWVLKDNRVTSALFGASNVEQIAENLRVMENLSFTAEEL"
     gene            complement(990..>2679)
                     /locus_tag="PKV59_07095"
     CDS             complement(990..>2679)
                     /locus_tag="PKV59_07095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP46554.1"
                     /translation="TAILSLQTFIGIVYYYSKENGSVSRAFKQLFWGGRIVWQVVLIF
                     LACLTLMSAFIPIRANYYPSFDSSIFSYIGQQILRGKLPYVDGWDHKPPLIFYIDAFG
                     LWLANGHLIGIWLLEVIALLLGSILFFRILKKYFSEGIALSVTMLGILHQARLFDFGN
                     YTEEFSLFFQLAAFGLAFSDRFRNRLGWQGFLSGILCGLAFTCKQNTVGLWAALFLLD
                     ILQLYLNRDQKSTSVHQVIYKWIRFLIGFLAVNGIWIVYFAVKGILREYWDVAFVYNF
                     VYAQKSGESRWATGLTTLTFLPSLSVFLLLAFLSWPAALFDRIRSFRQCNRNDRQKLF
                     DQNRLLLFALIALPVELILAGLSGMNYQHYFILCMPSACILAAWLGDSMLRQLSVRMK
                     TVAASLVVLAVFWIGSAPMANVYRRNYEPRMPSAATKTADFLNSNTDDGDLVQLWGGV
                     LAPYVMSERSSPSRFFNVRPLYLFPGYMQEEQWGTFLADLKNSPPKFIVYMNDRFLAQ
                     VPYNSNGFCEDLDLADYQRPTYQFLCDHYRYRETINEGMNDAWGVFERTGKETK"
BASE COUNT          834 a          666 c          531 g          648 t
ORIGIN      
        1 aagctcttct gcagtaaacg aaagattctc catcacccgc agattttcag caatctgttc
       61 cacattactg gcaccaaaca gcgcactcgt tacccgatta tccttcaaga cccatgccaa
      121 tgccatctgt gaaagcgtct gccctctctc aacggcgata ctgttcaatt gacgaatata
      181 accgatactc tcatccgtaa ttaaatcttt tttcagatgc cgatccaatg aggcgcgtga
      241 tccttccgga atgccgttta aatatctgga actcaacaaa ccgtgctgta gcggcgcaaa
      301 aacaattgtc cccatcccgt attcggcggt tatatcaaac agatgatccg tttccgtcca
      361 gcgattcaag atcgaataag aaggttggtg aatcagacaa ggtgtcccca attctctcag
      421 gatttccgca gctttttttg tcagatccgc gggataattg gaaattccga cataaagagc
      481 ttttccctga cgtaccagaa aatccaatgc tcccattgtc tcttccaacg gcgtttcggg
      541 atcgggacgg tgatgataaa acacatccac ataatccagc cccatccttt taagactttg
      601 gttcaggctg gcaaccagat acttttttga cccataatct ccgtaaggtc caggccacat
      661 atcatagccg gctttggtcg aaataaataa ttcatcccga taaggtttca gatcctgttc
      721 gtagatttgc ccgaataccc tttcggcggc gccgtaaggc ggcccgtaat tgttcgcaat
      781 atcaaaatgg gtaattccat gatcaaaagc ataacggatc atagcccgat cggtctcaaa
      841 acttttatac ccgtcaaaat tatgccagag tcccaacgtc aaaataggca gttgaacgcc
      901 gctttttccg cagcgccgat aaatcatatt gtcatagcga gcgggatctg ctgcatagac
      961 gcttgtcaaa ctcggatgat gtgcatcaat cattttgttt cctttccagt tcgttcaaaa
     1021 acaccccagg catcgttcat gccttcgttg attgtttctc gataacgata atgatcgcaa
     1081 agaaattgat aggttggtcg ctgataatcc gccaaatcaa gatcttcaca gaatccgttt
     1141 gaattataag ggacttgtgc caaaaaacgg tcattcatat aaacgataaa tttaggcggg
     1201 ctgtttttca gatcagccaa gaatgttccc cattgttcct cttgcatata accggggaac
     1261 agataaagcg gccgaacatt gaaaaagcgg gacggagagg atcgttcact catcacataa
     1321 ggcgccaaca cccctcccca aagctgaaca aggtctccgt catccgtatt gctattcaga
     1381 aaatcggcgg ttttggttgc ggcggaaggc atccgaggtt catagtttcg ccgatagaca
     1441 tttgccatcg gggcggagcc gatccagaaa acagctaata ccaccaatga tgccgcaacg
     1501 gttttcatgc ggacggagag ttggcgtagc atcgaatctc ccaaccaagc cgccagaata
     1561 caagccgacg gcatacagag aataaagtaa tgctgataat tcattccgga cagtcctgcc
     1621 aatataagct cgaccggtaa agcaatcaag gcgaacaata acaaacggtt ctgatcaaac
     1681 agtttctggc gatcattccg gttgcattgg cggaaactgc gaatccgatc aaacaacgcc
     1741 gcaggccatg acaaaaaagc caataataaa aacaccgata acgaaggtaa aaatgtcagt
     1801 gtcgtcagcc ctgttgccca tcgactctcc ccgctttttt gagcatagac aaaattataa
     1861 acaaacgcca catcccaata ttctcgtaaa attcctttta cggcaaaata aacgatccag
     1921 attccgttca ctgctagaaa tccgatcaga aatcggatcc acttataaat cacctgatgg
     1981 acggaggtag atttttgatc acgattcaaa taaagctgaa gaatatcaag taagaataat
     2041 gccgcccaca atccgaccgt attttgtttg caggtaaatg caagcccgca aaggatgccc
     2101 gagagaaatc cctgccagcc caatcgattc cgaaatcgat ccgaaaaagc caaaccgaat
     2161 gcagccaatt gaaagaacaa cgaaaattct tcggtatagt taccgaaatc aaataaacgt
     2221 gcctggtgca aaattcccaa catggttacc gaaagggcaa tcccttcgga aaaatatttc
     2281 tttaagatcc ggaaaaatag gatcgatccc agcaataaag cgatcacttc cagcagccaa
     2341 atcccgatca ggtgcccgtt tgccagccat aatccgaagg catcaatata aaatatcagc
     2401 ggcggtttat gatcccaccc atccacataa ggtaattttc ctctcaaaat ctgctgtccg
     2461 atataactga aaatcgagga atcaaaggaa ggataataat tcgctcgaat cggaataaat
     2521 gccgacatca atgtcaggca agccaagaaa attagaacaa cttgccaaac gattcttcca
     2581 ccccaaaata actgtttgaa agcccgtgag acggaaccgt tttccttaga ataataataa
     2641 acaatgccaa taaaggtttg taatgacaaa attgccgtc
//
LOCUS       DAOMIV010000294         2669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41835_length_2669_cov_4.487376, whole genome shotgun sequence.
ACCESSION   DAOMIV010000294 DAOMIV010000000
VERSION     DAOMIV010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41835_length_2669_cov_4.487376"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..>2669
                     /locus_tag="PKV59_07100"
     CDS             138..>2669
                     /locus_tag="PKV59_07100"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQP46555.1"
                     /translation="MEECVERDLTYSTPLYVTVLLAGADVSEPLKQDIFLGDFPEMTE
                     NGTFIINGSERVVVSQLIRSPGVYFEAPVDRTTGHRLAMAKLIPDRGAWMEFETRKND
                     YLILKFNRKRTVPITIFLRALSEVDDGIIGDSPLKNGSDEEIMALFADVDNNPDRMYI
                     PSTFKQEPDWEKDYPNMSISQAALLEFFKRMRPGDPVTVENAKQYVEEQLFDQRHYDL
                     ERVGRYKLNQKLDLTEYIPNNHRNVTKWDIVRLVRHLIMINNGMVEKDDIDHLGNRRV
                     KTVGELISNKLRIGLRRMERVIKERMSIRDQERVTPVSLVNIRPVVAALREFFGSSQL
                     SQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFDVRDVHHTHYGRICPIETPEGPNI
                     GLIGRLANFAKVNEYGFIETPYRRVYRNLPKTDDRLVGRQVTETLTAKNGKVIIEKGQ
                     RITEEMIPEIRKLDQSVIFITPYISDDVEYLSADAEDKFIIAQSNTRINEWHEFDEKS
                     IASRHYSDFTTYPPESIDYMDVSPQQVVGVSAALIPFLEHDDANRALMGSNMQAQAVP
                     LVNPDIPLVSTGMEKYAAVDSGQVLTSQEDGEVVSVNATQIIVRDNNGMLHTHQLRKY
                     QRSNQSTCIDQRPAVIKGQKVKKNEVLADSSSTVEGQLALGQNMVVAFVSWEGGNFED
                     AILISERVVMDDKFSSVHIEKYELEARDTKLGPEEITRDIPNVGDDAIKDLDENGIIR
                     IGAEVGPNDILVGKITPKGEKELTPEERLLRAIFGEKSRDVKDTSLKMPHGERGKVVD
                     VKVFTREENSDLSAGVDTMVRVYVAQRRKLTAGDKMAGRHGNKGVV"
BASE COUNT          743 a          613 c          667 g          646 t
ORIGIN      
        1 aaggcatcgc agaacttctt gatgaaattt cccctattga atctttcaat aaaggaatga
       61 aactttattt tccgagcaag gaccctatct ctgaagaatg gggtttgaaa ttctggtttg
      121 gcgaaccgaa gcattcagtg gaagaatgcg ttgaacggga tctcacttat tctacgccgt
      181 tgtacgtaac agtgttgtta gccggcgcag atgtctccga accgttgaag caggatatat
      241 ttttgggcga ctttcccgaa atgacagaaa acgggacgtt tatcatcaac ggctcggaaa
      301 gagtcgttgt atctcagttg attcgatctc caggtgtcta cttcgaagca ccagtcgatc
      361 gcaccaccgg tcaccgattg gcaatggcaa aattgattcc ggatcgcggc gcctggatgg
      421 aatttgagac ccgaaagaat gattacctaa ttttaaaatt caatcgaaag cggacagtac
      481 caattactat ttttctgagg gcgctgtctg aagtcgatga cgggatcata ggagattcac
      541 cgctcaagaa tggttccgat gaagaaatca tggcgctttt tgctgatgta gataacaatc
      601 cggatcggat gtacattccg tcgacattca aacaggaacc ggattgggaa aaagattatc
      661 cgaacatgtc catttctcag gcagctttgc ttgagttctt caaacggatg cgccccggtg
      721 atccggtcac ggtagaaaac gcaaaacagt acgttgaaga gcaacttttc gatcaacgcc
      781 attacgacct tgaacgggtc ggacgatata aattaaatca aaagctggat ctgaccgagt
      841 acattccgaa caatcatcgg aatgttacca aatgggatat cgtccgtctt gtccgccatt
      901 tgatcatgat caacaacggt atggtcgaaa aagatgatat tgatcacctc ggtaaccgtc
      961 gtgtcaaaac agtcggtgaa ttgatttcta ataagctgcg catcggttta cgccgaatgg
     1021 aacgggttat caaggagcgg atgtccatcc gcgatcagga aagggtcaca cctgtcagtt
     1081 tagtcaatat tcgaccagtt gtggcagctt taagagaatt cttcggctcc tctcaacttt
     1141 ctcagttcat ggatcagacg aatccgttag ccgaattacg ccataaacga acgctttcgg
     1201 ctttgggccc cggcggtctg cggcgggaac gtgccggatt cgatgttcgt gatgtccacc
     1261 atacccatta cggccggatt tgtccaattg aaactcccga aggtccgaac atcggcctga
     1321 tcggccggtt ggcgaatttt gccaaagtca acgaatacgg cttcatcgaa actccatacc
     1381 gtcgcgtcta tcggaatcta cccaagaccg acgacaggtt ggtcggacgg caagtcacgg
     1441 aaacactgac cgcaaaaaac ggaaaagtca ttatcgaaaa aggtcagcgg attacggaag
     1501 aaatgattcc ggaaattcgg aaactcgatc agtccgtgat cttcattaca ccgtatattt
     1561 cggatgatgt cgaatatctt tcagcggatg cggaggataa attcatcatc gctcagtcta
     1621 atacccgaat caatgagtgg catgagtttg acgaaaagtc aatcgcaagc cgccattatt
     1681 ccgatttcac aacctatccg cccgaatcca tcgattatat ggatgtctct ccgcagcagg
     1741 ttgtcggtgt cagcgcggcg ctgattccgt tcttggaaca cgacgatgcc aaccgtgcgc
     1801 tgatgggttc caacatgcag gcgcaagccg ttccgttagt aaatccggac attccgctgg
     1861 tctctaccgg aatggaaaaa tatgcggccg tcgattccgg tcaagttttg acttctcagg
     1921 aagacggcga agtggtttcg gtcaatgcga cccaaatcat tgtccgggat aacaacggaa
     1981 tgctgcatac acaccaactg cgaaaatatc aacgttcgaa tcagagtacc tgtatcgatc
     2041 aacgtccggc ggtaatcaag gggcagaaag tcaagaaaaa tgaagtgctg gccgattcct
     2101 cttcgactgt cgaaggacaa ttggctttag gacaaaatat ggtcgtggct ttcgtctcct
     2161 gggaaggcgg gaactttgaa gacgccatct tgatttccga aagagtggta atggatgaca
     2221 aattctcttc cgttcatatt gaaaagtatg aattggaagc ccgcgatacc aagcttggtc
     2281 ccgaagaaat cacacgggat attccgaatg tgggtgacga tgccatcaaa gacctggacg
     2341 aaaacgggat tatccgaatc ggtgccgaag tcggtcccaa tgatattttg gtcgggaaaa
     2401 tcactcctaa aggggagaaa gaattaactc cggaagagcg tctgctgcgg gcgattttcg
     2461 gcgaaaaatc ccgtgatgtc aaagatacat ccctgaaaat gccgcatggc gaacgcggaa
     2521 aagttgtgga cgtcaaagtt tttacccgcg aggaaaacag tgacctttcc gccggtgtgg
     2581 acaccatggt tcgcgtctat gtcgctcaac gccggaaatt aaccgcaggc gataaaatgg
     2641 cgggacgtca cggaaacaaa ggcgttgtt
//
LOCUS       DAOMIV010000295         2666 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41909_length_2666_cov_3.603217, whole genome shotgun sequence.
ACCESSION   DAOMIV010000295 DAOMIV010000000
VERSION     DAOMIV010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41909_length_2666_cov_3.603217"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PKV59_07105"
     CDS             complement(<1..616)
                     /locus_tag="PKV59_07105"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HQP46556.1"
                     /translation="MQQLISELLFLGQRFNRVSVIDILLVASIFFVILRFIRSSQANT
                     LLRGTITIFIIIALLYVLVDLPAFTWLIGNILPVLLIVIPVVYAPEIRRAFERMGRVQ
                     SIHDLFASAPPHAEEMNQVIDAVVTACSMLAARNHGALIVMQRYDDLEKFIETGVYLN
                     AVVSPELLLQIFYPNTPLHDGAVIIRKGRIEAAVCVMPLSSRNVL"
     gene            complement(758..1354)
                     /gene="coaE"
                     /locus_tag="PKV59_07110"
     CDS             complement(758..1354)
                     /gene="coaE"
                     /locus_tag="PKV59_07110"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQP46557.1"
                     /translation="MKKLVIGITGGIASGKSEIRRELESLGCLGIDADRIGHEVLEPE
                     SSVAEDVLRHFGSRILSDRNTGKIDRKKLGQIVFENPAELKWLESVTHPQIIRRINQI
                     IAETDRSVALEAVKLSDSGLSSLCVQKWLVETDPEIQTERLMRKRGLSREDAQIRIRM
                     QQNMDWHRNEMDHIFNGGIPLADLKAEVRKVWYALQDH"
     gene            complement(1378..>2666)
                     /locus_tag="PKV59_07115"
     CDS             complement(1378..>2666)
                     /locus_tag="PKV59_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQP46558.1"
                     /translation="PGVVIMLMFLCLFSFFLLKGVLTGQIVSPWKPTAIPTRDAASYA
                     MEAEAQFVAGNLEKAVEAYQAAANLEPTNPEYIWKQGRALAYMTASQTTDMEKKETLE
                     KAIKLLESSLETFKENSDIYAVLAMVYLWYADKDICGADMVETNMATAESYIRRAVDY
                     GPRNAMAYAFYSEILLDQANYERAEQMLSQALEYGTANGSDMFDVYRVQGNLLESYGQ
                     YRSAIDAYNRALALSPNMTFLQIRIGVNWRQIQEYDAALEMFSKAARINDQLGINDPL
                     PYLAIGNTYSQTGDFYAAGINIKKALSINPYNADVYGRLGVNYYKARNYEAAIEALKC
                     ATYGCGAVESCAVRECDDPGNPLIEISGLPLSGTSVIYYFTYAAALSYMHTNANHYCE
                     EAVKITKEVSKQFGTDETVMYIVRDSEAICASYGIK"
BASE COUNT          661 a          640 c          669 g          696 t
ORIGIN      
        1 ccagaacgtt tcgtgaggac agcggcatca cgcagacggc ggcttcgatg cgccccttgc
       61 gaatgatgac ggcgccgtca tgcagggggg tgttcggata aaagatttgt aacaggagtt
      121 cgggtgaaac gaccgcattc agataaacgc cggtctcgat gaatttctcg agatcgtcat
      181 agcgctgcat tacaatcaac gctccatggt ttctggcggc gagcatggaa caggcagtga
      241 ccaccgcatc gatcacctga ttcatttctt cggcgtgggg cggtgcggac gcaaacaagt
      301 catggataga ttgaactctc cccatgcgtt caaaagctct gcggatttcc ggcgcataga
      361 caaccggaat gacgatcaaa agaaccggaa gaatgttgcc gattaaccac gtgaaagccg
      421 gcagatcaac cagaacatag agcaatgcga taataatgaa aatggtgatg gttccccgca
      481 aaagggtgtt tgcctgggaa cttctgataa agcgcaggat gacaaagaaa attgacgcaa
      541 ccaagagtat atcgatcacg cttacgcgat taaatcgctg accgaggaat aataactctg
      601 aaatcagttg ctgcacatta gcctctcatc ctttttttca aatttgaaac tttccttacc
      661 attgtaacta ttttcaacgg tagtagtcaa ggaaaatcaa atctgtgatt aaaagttcct
      721 taaaaaagga agaatttcaa ataaaaaaca gaaaaaatta atggtcttga agggcgtacc
      781 aaactttgcg tacttcggct ttcaggtccg ctaacggaat gccgccatta aatatatgat
      841 ccatttcatt ccgatgccaa tccatatttt gttgcattcg tattcgaatc tgcgcatctt
      901 cccgtgaaag ccctcttttt cgcatcagtc gttccgtctg aatttccgga tccgtctcga
      961 ccagccattt ttgaacgcag agtgaagaca atcccgaatc gctcagtttg acagcttcca
     1021 acgccacgga gcgatccgtt tccgcaatga tttggttgat acgccggatg atctgcggat
     1081 gggtgacgga ttccaaccat ttcaattcgg cgggattctc gaagacgatt tgacccaatt
     1141 tttttcgatc gatttttccg gtatttcgat cggagaggat ccggctgccg aagtgacgga
     1201 gcacatcttc cgcaaccgaa gattccggtt cgaggacttc atgaccgatt cggtcggcat
     1261 cgatgccgag gcaacccagt gactccaact cgcggcgaat ttcgcttttt ccgcttgcga
     1321 ttccaccggt aataccgatg acgagttttt tcatttccgg ttgtttccct tccgcaatta
     1381 tttgataccg taagaggcgc aaatcgcttc gctgtcccgt acgatgtaca tgacggtctc
     1441 gtcggtgccg aattgtttcg agacttcttt ggtgattttt acggcttctt cacaataatg
     1501 attggcgttg gtgtgcatgt aagacagagc ggcggcatag gtgaagtaat aaatgacgct
     1561 ggtacccgat aagggcaatc cggatatttc gatcaaagga ttgccgggat cgtcgcattc
     1621 gcggacggcg cagctttcga ccgcgccgca gccataggtg gcacatttga gagcttcgat
     1681 ggcagcttcg tagttacggg ctttatagta gtttacgccc aaccgtccat agacatcggc
     1741 gttgtaagga ttgatgctga gtgctttttt aatattgatt ccggcggcat agaaatcacc
     1801 ggtctgggaa taggtgttgc cgatagcgag gtaaggcagc gggtcgttga tcccgagctg
     1861 atcgttgatt cgagcggctt ttgagaacat ttccagcgcg gcatcatatt cctgaatctg
     1921 gcgccagttg acgccgattc ggatttgcag gaaggtcatg ttaggagaaa gtgcgagcgc
     1981 gcgattatag gcatcgatgg cgctgcggta ttgcccgtag gattccagca ggttgccctg
     2041 aacccgatag acatcaaaca tatcgctgcc gtttgcggta ccatattcca atgcctggct
     2101 gagcatttgt tcagcccgct cataatttgc ctggtccaac aggatttccg aatagaacgc
     2161 atacgccatt gcatttcgcg gaccgtaatc caccgcccgg cggatatagg attcggcggt
     2221 ggccatgttg gtctccacca tgtcggcgcc gcaaatgtct ttatccgcat accaaaggta
     2281 aaccatcgcc aaaacggcgt agatatccga attctcttta aaagtttcca acgagctttc
     2341 taacagtttg atcgcttttt ccagtgtctc tttcttttcc atgtcggtgg tttgagaggc
     2401 ggtcatataa gccagtgccc gtccctgctt ccaaatatat tccggattgg ttggttccag
     2461 attggccgcg gcttggtatg cttcgaccgc tttttcgagg ttccctgcca caaattgagc
     2521 ttccgcttcc atggcatacg aagccgcatc acgggtgggg atggcggtgg gtttccatgg
     2581 ggaaacaatc tgaccggtta aaactccttt aagcagaaaa aagctgaaga gacagaggaa
     2641 cattaacata ataaccactc cgggat
//
LOCUS       DAOMIV010000296         2665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_41923_length_2665_cov_3.731801, whole genome shotgun sequence.
ACCESSION   DAOMIV010000296 DAOMIV010000000
VERSION     DAOMIV010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41923_length_2665_cov_3.731801"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..1530)
                     /locus_tag="PKV59_07120"
     CDS             complement(208..1530)
                     /locus_tag="PKV59_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQP46559.1"
                     /translation="MFNKKNLFVIIVLLLLMTLYTPGFAAAGLDDIDWTVYHDVDFYG
                     QTLHALAENYPDIVKVYTIGHSWRERQLWTAEITGTGTEPKTGIYIVGNIHGGENEGA
                     ISAMYFAWWLVTNSEDAAVKDILDKYIIYVTPVMNPDGYAASAIYNTRQNLRPKDANG
                     DGKVFSDPYTDTNGDGFISEIYTGAQDSKVEDRVYVGMESPDWDKNGIPGDDPKNSGI
                     DLNRTFDYMWNYLDVDTDPILGEDIYKRAGPDAASEPEVQAVQNFMIYHPVYAVLSLH
                     TGEQSVLYPWCYTQEPTKDNDFFKGVAQKMIAAYEDVTGRPAYEKQSAHDYQTDSEMI
                     DWAYGRLGIHAYTMEVYAPGEPATEGDDSETHTWGNKLPEEKWEYVGDWQGLKDVWFK
                     TSPTAQMVRTAPPDQDKLVEGTKNAILELIKSEPNGDGPVVPEYLKWE"
     gene            complement(1745..>2665)
                     /locus_tag="PKV59_07125"
     CDS             complement(1745..>2665)
                     /locus_tag="PKV59_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQP46560.1"
                     /translation="SIRIELDGTVTLRAGVPDIGGGQASVLAEIVAEELGLNIEDVHP
                     FIMDTHLTPLCGTTTATRQLYMSGNAAMKAADVMRDRILNEASKILKYPVESLALGDR
                     KVYLVNNPTVNVPFVTVVNSLSNNGGMLETIEQFNAPFTDVPNLKDIKGEVLPDLTYT
                     CHGVEVAVDEETGVFEILRIIAGIDAGKMVNKNSCEGQVEGGAVYHSLWATHEDLQWR
                     NGITKANNFSTYIIPTSVDIPDIETIILESGGGFGPYGAKGVGEPSDNSIAPAVINAI
                     RDAIGTDLNLNKMPITYEKIMCACKGIKID"
BASE COUNT          736 a          641 c          487 g          801 t
ORIGIN      
        1 ttgcttggga aagcaaaaag atttacagtt cgcgttgaat ggattagccg aaattttagg
       61 ggcgccggca ccgcttatat ttgatctttg aaccataaag tttcttaaac ttaaaagaag
      121 aaatccatgc ttaattccac atggatttct ttttacgata tcggcttctt ttagatttgt
      181 attatgattt gaaacacgct acacgcttta ttcccatttt aaatattcag gcactacagg
      241 tccgtcccca ttcggttcgc tcttaatgag ttcaagaata gcattcttcg tgccttccac
      301 caacttatcc tgatcgggag gagccgtccg taccatctga gccgtggggc ttgtcttgaa
      361 ccatacatct ttcagacctt gccaatctcc gacatactcc catttttctt ccggcagttt
      421 atttccccaa gtatgagttt cagaatcatc gccttcggtt gcgggttccc ctggagcata
      481 gacttccatc gtatatgcgt gaatgcccaa tctgccgtat gcccaatcaa tcatctctga
      541 atccgtctga taatcatgag cggattgctt ctcataagcg ggtcgtccgg tcacatcttc
      601 gtaagcggca atcatttttt gagcgacgcc tttgaaaaag tcattatctt ttgtcggttc
      661 ctgagtatag caccaaggat aaaggacact ttgttcgccg gtatgtaaag aaagaacggc
      721 ataaaccgga tgataaatca taaaattctg tacagcttgg acttccggtt cactggcggc
      781 atcgggcccc gccctcttat atatatcttc cccgagaatc ggatccgtat ctacatctaa
      841 gtaattccac atatagtcaa aagttcgatt cagatcgata ccgctgttct tgggatcatc
      901 tccgggtatg ccattcttat cccaatcggg actttccata cccacataaa ctcgatcttc
      961 aacttttgaa tcctgagcgc cggtataaat ttctgaaata aagccgtcgc cgttggtatc
     1021 tgtataagga tcgctgaaca cttttccgtc accgtttgca tcctttgggc gcaaattttg
     1081 ccgggtatta tagattgccg aagcggcgta tccatccgga ttcattaccg gcgttacata
     1141 aataatatac ttatccaaaa tatcctttac agcggcatct tccgaattcg ttacaagcca
     1201 ccatgcaaag tacatcgccg aaatcgcacc ttcattttca ccgccatgaa tattcccgac
     1261 gatataaatt ccggtctttg gttctgtgcc ggtgcccgta atttcagccg tccataattg
     1321 gcgttcgcgc caactgtgac cgatcgtata tacttttaca atatccggat aattttcagc
     1381 caatgcgtgc agcgtctgcc cataaaaatc aacatcatgg taaacggtcc aatctatatc
     1441 atctaaaccg gccgctgcga atcccggagt ataaagcgtc ataagcaata acaatacgat
     1501 aataacaaaa aggtttttct tgttaaacat aaaattctcc tgatctaata aacgaacaaa
     1561 aagttcctct gaagccgata tattcattat agattgtgcg aaaattagca attagtaatt
     1621 ttcgtaatga aaaaacagca taaatgtcat tcttaattat tatctataat tattattcta
     1681 ttatttaata aaataagagg ctgatcatca atttgtcagc ctcttatttt atgaattctt
     1741 cgatttaatc gatttttatt cctttacagg cacacattat tttttcgtaa gtaatcggca
     1801 ttttatttag attaagatct gttccgattg catcgcgaat agcattaatg accgccggag
     1861 caatagaatt atcagacggt tcacccactc ctttcgctcc ataaggacca aatccgccac
     1921 cactttcgag aataatcgtt tcgatatccg ggatatcaac tgatgtcgga ataatgtagg
     1981 tagaaaagtt attcgccttg gtaattccgt tccgccattg caaatcctcg tgcgtcgccc
     2041 ataaagaatg ataaacagca ccgccttcca cctgaccttc acaagagttc ttatttacca
     2101 tctttccggc atcgattccc gcaataattc ttagtatttc gaatacaccg gtttcttcat
     2161 cgacggcaac ttctacacca tggcaggtgt aagtcaaatc cggcaaaact tcgcccttta
     2221 tgtctttcaa attcggaaca tccgtaaaag gagcattgaa ctgttcgatc gtttccaaca
     2281 ttccaccgtt gttacttaaa ctattgacaa ccgttacaaa gggaacatta acagtcggat
     2341 tattgacaag atatactttg cgatcgccca aagcaaggct ttcaaccgga tattttaaaa
     2401 tcttggatgc ttcgttcaga atccggtcac gcataacatc agcggctttc atcgcagcat
     2461 ttcccgacat atagagctgt cgggtggcgg ttgtcgtccc gcaaagcggt gttaaatgag
     2521 tatccatgat aaagggatgg acatcttcaa tatttaaacc caattcttct gcgacaattt
     2581 ctgccaacac acttgcctgt ccgccgccga tatccggcac gcccgcgcga agcgtcacgg
     2641 taccgtccaa ttcgatacga ataga
//
LOCUS       DAOMIV010000297         2662 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_42001_length_2662_cov_3.141158, whole genome shotgun sequence.
ACCESSION   DAOMIV010000297 DAOMIV010000000
VERSION     DAOMIV010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42001_length_2662_cov_3.141158"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="PKV59_07130"
     CDS             <1..767
                     /locus_tag="PKV59_07130"
                     /inference="COORDINATES: protein motif:HMM:NF039324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HQP46561.1"
                     /translation="MILTLIILYSAFLFVILPKMNISQVKVQDIFSIPFQQTARYLKT
                     YPSDITDQEAEAIDRILEYNKIAENYNPESSDPVKSLYRQNARADDFINYFHAWFTMF
                     MRHPSVYIQATLNNTYGYFYPDEPGKSEDYVFWGIKSYLTTDKVNLFNPEKLNDARSI
                     LFQIIYTFRVFPGISMLSSIGFHVWLLILSFSYLIMIHQYHYIIGLIPSILSLFVCLL
                     SPINTYSRYAWPIIFLTPLVIFICIPTCREPIIKEK"
     gene            854..1450
                     /gene="rdgB"
                     /locus_tag="PKV59_07135"
     CDS             854..1450
                     /gene="rdgB"
                     /locus_tag="PKV59_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQP46562.1"
                     /translation="MRQKILIATNNLNKIREIQNILSDFDLITPQELGIDLEIEENGN
                     TFEENAEIKAESFCKASGMLSLADDSGLEVECLGGEPGVHSHRYCPKPNATDADRRAF
                     LLENLKGKPRPWKARFYCAAALCDPKSSEIQLVHGICNGEIIPEERGNGGFGYDPIFW
                     IPERGMTLAEMSENEKNEISHRGAAMRKIREILKKKDV"
     gene            1457..>2662
                     /locus_tag="PKV59_07140"
     CDS             1457..>2662
                     /locus_tag="PKV59_07140"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HQP46563.1"
                     /translation="MDTIRHLYRIGHGPSSSHTMAPRRAAEMFKARYPDAEHYTVVLY
                     GSLAATGKGHLTDKAIYEVLGKKKTEIIWKPDYVPAFHPNGMEFSAWDGQKQLINKWM
                     VYSVGGGALSEGPQDSEVGLFYPHRNMAEIMAHLQRTGKSFWEYVEDCEGKGIWDYLR
                     EVQKTMYAAIDRGINREGVIPGGLGISRKAPIYFRRGRLMNPTMASVGRLTSYALAVS
                     EENATGGLVVTAPTCGSSGVVPAIIRYLQENLNHSDETILKALATAGLFGNVVKHNGS
                     ISGAEVGCQGEVGVAAAMAAAAAAQLYSCSIRQIEYAAEMALEHHLGLTCDPVHGLVQ
                     VPCIERNAFAAGTAIYVAEYVSFTDGSHLVSFDQVVEVMMQTGVDMPSLYRETSEGGL
                     AKFFKTPKKN"
BASE COUNT          755 a          521 c          629 g          757 t
ORIGIN      
        1 taatgatctt gactttgata atactttatt ccgcattttt atttgttatt cttcccaaaa
       61 tgaatatttc acaggtaaaa gtgcaagata tattctccat cccgtttcag cagactgccc
      121 gatatttaaa aacctacccc tctgatatca cagatcagga agcagaggca atcgatcgaa
      181 ttttagaata taacaaaatt gccgaaaact ataatccgga aagttccgat ccggttaaaa
      241 gtttatatcg acagaacgca agggctgacg attttataaa ctattttcat gcatggttta
      301 ccatgtttat gcgccatccc tctgtctata ttcaagccac actcaacaac acttacggat
      361 atttttaccc cgatgagccg gggaaatccg aagattatgt tttttggggg ataaaatctt
      421 acctaaccac ggataaagtt aatttattta accctgagaa actgaatgac gcacgaagta
      481 ttttatttca aataatttac actttccgtg tttttcctgg aatttcaatg cttagcagta
      541 tcggttttca tgtctggtta ctaatccttt cattttcgta tttaataatg attcatcaat
      601 atcactatat tatcggattg attccttcaa tactatcgtt gtttgtttgc cttttgtctc
      661 ctatcaatac ttattcccgt tatgcttggc cgattatctt tttaacccct ttagttatat
      721 ttatttgtat accgacttgt cgagaaccga ttattaagga aaaataatca acgtaatcac
      781 agcttgattt ggcgttacta atctctgaat aatgaagcgt acaatgtttt tttattgata
      841 gaatagttta gacatgagac agaaaatatt gatcgcaacc aataatctta ataaaatccg
      901 ggaaattcaa aatatcctga gcgatttcga tttaattacc ccgcaagagc tcggaattga
      961 tttggaaatt gaagagaacg gaaatacatt cgaagaaaat gccgaaatca aagccgaatc
     1021 tttttgtaag gcgagcggta tgttatcgct ggcagatgat tccggcttgg aagtggaatg
     1081 tttgggtgga gaaccgggag ttcattctca ccgatattgc ccaaaaccga atgccaccga
     1141 tgcggaccgg cgagcgtttt tactcgaaaa tttaaaaggg aaaccgcgtc cgtggaaagc
     1201 caggttttat tgtgcggctg ctttatgcga tccgaaatcg tcggaaatcc aactggttca
     1261 cggaatttgt aacggcgaga ttattcccga agaacgtgga aacggcggct tcggctatga
     1321 tccgattttt tggataccgg aacgcggaat gactttggcg gagatgagtg aaaatgaaaa
     1381 aaatgaaatc agtcatcgcg gagcagccat gcgaaaaata cgggaaatac taaaaaagaa
     1441 agatgtttga ttaagaatgg atacaattcg ccatttatat cggatcgggc acggtcccag
     1501 cagtagtcat accatggcgc ctcgaagagc tgccgagatg tttaaagctc gttatccgga
     1561 tgcggaacat tatacggtcg tcttatacgg aagtttggct gcgaccggta aaggtcactt
     1621 gaccgataaa gcgatttatg aagttttggg aaaaaagaaa accgaaatta tttggaaacc
     1681 ggattatgtt ccggcatttc atccaaacgg gatggaattt tccgcctggg acggacaaaa
     1741 gcaattgatt aacaaatgga tggtttattc ggtgggcggc ggagcactct cagaaggtcc
     1801 gcaagattct gaagtcggtt tattctatcc ccatcgaaac atggcggaaa taatggcgca
     1861 ccttcaacga accgggaagt cattttggga atatgtggaa gattgcgagg ggaaagggat
     1921 ttgggactat ttgcgtgagg tccagaaaac gatgtatgct gcgatcgatc gcgggatcaa
     1981 tcgggaaggt gtcattccgg gaggactggg aatttcccgc aaagctccga tctactttcg
     2041 acggggcaga ttgatgaatc cgacgatggc atccgtaggt cgtttgacct cttatgcttt
     2101 ggctgtttcg gaagaaaacg ccaccggcgg gctggtggtt acggcgccga cttgcggatc
     2161 cagcggggtt gtcccggcaa ttattcgcta cttgcaagaa aatttgaatc attcggacga
     2221 aacaattctg aaggctttgg cgacagccgg attgttcgga aatgtggtaa aacataacgg
     2281 atctatctcc ggtgcagagg taggttgcca aggtgaagtc ggggttgccg cggctatggc
     2341 tgcggctgcg gctgcacagc tttattcctg ttctattcgg caaattgaat atgctgcgga
     2401 aatggcgttg gagcatcatt tgggattaac ctgcgaccct gtacacggtc tggttcaagt
     2461 tccttgcatc gagcgaaatg ctttcgctgc cgggaccgcc atctatgtag cggagtatgt
     2521 cagttttaca gacggttcgc atttggttag ttttgatcag gtagtagaag tcatgatgca
     2581 gaccggggta gatatgccct ctttataccg tgaaaccagt gaaggcggac tggcgaagtt
     2641 ttttaaaaca cccaaaaaga at
//
LOCUS       DAOMIV010000298         2641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_42463_length_2641_cov_4.014308, whole genome shotgun sequence.
ACCESSION   DAOMIV010000298 DAOMIV010000000
VERSION     DAOMIV010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42463_length_2641_cov_4.014308"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..636
                     /gene="rpmF"
                     /locus_tag="PKV59_07145"
     CDS             439..636
                     /gene="rpmF"
                     /locus_tag="PKV59_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQP46564.1"
                     /translation="MTPLPKRKLSSARRNRRRANDFLTANNLTQCPNCGEMRLPHTVC
                     PSCGYYRGREVINMTKETKSE"
     gene            complement(698..2017)
                     /gene="hflX"
                     /locus_tag="PKV59_07150"
     CDS             complement(698..2017)
                     /gene="hflX"
                     /locus_tag="PKV59_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQP46565.1"
                     /translation="MMENELKLTQKPAERAFLVGAEIKNQPGLLSIADSLEELRLLSE
                     TAGLEVVGETSQKLDKPNPATYIGSGKVEEVRALAEETLADVVIFDNELSPRNLRDLS
                     KVLGDSVRVIDRTALILDIFAQHAKTREGALQVELAQYEYRLPRLTHAWAHLERQGAG
                     ASGRTGSVGGVGLRGPGETQLEVDKREIRKRIAVLKKQIEKMSDHRNLYRNQRKHSQI
                     PIVSIVGYTNAGKSTLLNLMSNSDIYTADKLFATLDPTTRRVNLPGNHLSLFTDTVGF
                     IQKLPTQLIAAFHSTLEEITEADLLLHIIDISHPNALAQFESVRETLKEIGAENIPIL
                     NVFNKADLLEDAEEAKNLLAETDPNAYLISAKTGQGIDELKTGIMEQLFEKMVPIRVR
                     LPFREGALIALFHEEGIVESVENESGGVVIQGAVPGRYLTRFQKFLV"
     gene            2235..>2641
                     /locus_tag="PKV59_07155"
     CDS             2235..>2641
                     /locus_tag="PKV59_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46566.1"
                     /translation="MMKTEKIQLQPFDNIDSILDKLNQTYQATYGLTQIQILWPRRGK
                     LLDDPLDFGRISGWAERNHVKLVFVSSNEQIMRKAAEHNIPVCPEVDSIPAAVTNRKD
                     RFPMKRSESVRAIRIAKLKSERDQLREKKVPVSS"
BASE COUNT          751 a          569 c          583 g          738 t
ORIGIN      
        1 cgaaatgtag gcatcccagt tgcaaagaag ataggaaaat gtatagggga taatgaaata
       61 aatcctcgga cattatcgcc tcacctgctc ggcgcgggac gtcgaggacg ccgtccccta
      121 cggatgatga taaattgtgg gatattatcg cctcgcatgt tcggcgcggg acgtcgagga
      181 cgccgtcccc aacggatggt gataaataaa atggtgttat cggttcattg taatcaaata
      241 tgtatttcgt ttgggaaatt taaattttca aagatgtgaa tgcaattcta tatacgttta
      301 cttttactga ttccaatttt aaaattctgt catcagagag cagattcaca gttgacgaag
      361 tggtactggt tgaatataat tagcttcgcc ttgttgaacg ggtatattta tggtggatag
      421 aaatgaggag tttataaaat gacaccgcta ccaaaacgaa aactttccag tgcacgcaga
      481 aatcgccgcc gcgcaaatga ttttttgact gccaataatc tgacccagtg tccgaattgc
      541 ggtgaaatgc gtttaccaca taccgtatgt ccgagttgcg gatactaccg cggtcgtgaa
      601 gttattaata tgacaaaaga aacgaagagc gaataatttt tttcgtaatg aaaaacgacg
      661 gattccgaaa agaatccgtt tttgttttaa taatttctta taccaaaaat ttttggaatc
      721 gggttagata acgccccgga acagcccctt ggatcacaac cccaccggat tcgttttcga
      781 ccgattcaac gattccttcc tcatgaaata aggcgatcaa agccccttcc ctgaacggaa
      841 gacgaacacg aatcggaacc attttttcaa ataattgttc cataatacct gtttttaatt
      901 catcgatacc ttgcccggtc tttgcggaaa tcagataagc gttgggatca gtttcggcga
      961 gcaggttttt cgcttcttcc gcatcttcca aaaggtccgc tttgttaaat acattgagta
     1021 tgggaatatt ctcggcaccg atttctttca atgtctcccg aacactttca aattgagcca
     1081 gagcattcgg atgagagatg tcgattatat gaagtaagag atccgcttcg gtgatttctt
     1141 ccaaggtgga atgaaaagcc gctatcaatt gtgtcggaag tttttggata aagccgacgg
     1201 tatcggtaaa gagagaaaga tggttgcccg gaagattaac acgtcgggtg gtcggatcga
     1261 gtgtcgcaaa gagcttatca gcggtataaa tgtccgaatt cgacatcagg ttcagcagtg
     1321 tcgatttccc ggcatttgtg taaccgacaa ttgatacaat cggaatttgt gaatgtttcc
     1381 gctgattgcg atacagattg cggtgatcgg acatcttttc aatctgcttc ttcaaaaccg
     1441 ctatccgctt acgaatttct cgcttatcga cttccaattg tgtttcaccg gggccgcgca
     1501 gcccaactcc tccgacgctg ccggttctgc ctgaagcgcc ggctccctgc ctttccagat
     1561 gtgcccaggc atgcgtcagg cgcgggagcc ggtattcata ttgggctaat tccacctgta
     1621 aagcgccttc gcgggttttt gcatgttgtg caaatatatc caaaattaat gccgtacgat
     1681 cgatcacccg aaccgagtcg cccaagactt tggataggtc tctcaagttt cgcgggctga
     1741 gttcattatc aaaaataacc acatccgcta aagtttcttc ggccaaagcg cgcacttctt
     1801 ccactttccc gctgccgata taggtagcgg gattgggctt atccaatttt tggctggttt
     1861 ctcccacaac ttctaatccg gctgtttcgg aaagcaaacg taattcttcc aatgaatccg
     1921 caatcgataa caaacccggc tgatttttaa tctcggctcc gactaaaaaa gcccgttccg
     1981 ccggtttttg tgttagtttt aattcatttt ccatcatata gcaacgatta tactgagaaa
     2041 aattcggttg aggtgaaaca aaaatgaaac cgataccttg gtttttttcg attcgtcatc
     2101 gcaacatgat taaaaatgtc tcattgatat attaaatcgt tttcttcctg accggataag
     2161 aatttcgaat atcgatttta aaagaatcaa ggatagaaaa aaacggcaaa aataagactc
     2221 aggtataatt tttcatgatg aagaccgaaa aaattcagtt acagccattc gataatatcg
     2281 actctattct ggataaactg aaccaaacgt atcaggcaac ttacggctta acgcaaatcc
     2341 agattttatg gccgaggcgg ggcaaattac ttgatgaccc attggatttc gggcgaatca
     2401 gcggctgggc ggaacgaaat cacgtcaagt tggtctttgt gtcttccaat gaacaaatta
     2461 tgaggaaagc ggcggaacat aatattccgg tttgtccgga agttgattcg attccggcgg
     2521 ctgttacaaa tcggaaagat cgtttcccaa tgaaacgatc ggaaagcgtc cgtgcgatca
     2581 gaatcgcaaa actgaaatcg gaaagggatc agctcagaga aaaaaaagta cccgtttcat
     2641 c
//
LOCUS       DAOMIV010000299         2639 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_42505_length_2639_cov_3.764706, whole genome shotgun sequence.
ACCESSION   DAOMIV010000299 DAOMIV010000000
VERSION     DAOMIV010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42505_length_2639_cov_3.764706"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PKV59_07160"
     CDS             <1..759
                     /locus_tag="PKV59_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46567.1"
                     /translation="FGSGKNAVNAVIDVDFQIHQGEIISIVGTSGSGKTTLAKILLGL
                     LKETSGEILYKGKPRLLKSQKERRAYWQNIQAIFQDPFSTFNQFFRVDKILNDCLKLQ
                     GLEHISQKEKTEKMQDACKFVNLKYEELYNKYPFELSGGQMQRLMIARIFMVKPNILI
                     ADEPTSMIDACSRSTILDMLMKLREEEGMTILFITHDLGLAYYLSDTIYIMESGRIVE
                     KGPAEDVILHPQDSYTKQLVSDVPKLHEEWVLSY"
     gene            complement(791..1810)
                     /locus_tag="PKV59_07165"
     CDS             complement(791..1810)
                     /locus_tag="PKV59_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQP46568.1"
                     /translation="MNCYERVMNRLAGKPVDRIPNLCILMTFAAKYIHVPYDQFVQDY
                     RLLVEGNIRCCEDFKIDLLSAISDPVREASDLGAAIAFPVDDVPHPTVPFVSCSADLA
                     KLKIVKPEDGRRMSDRLAAVSLYREKAGKEYPVMGWVEGAFAEACDLDSVSNIMISVV
                     DDPEFTAELLEFCTQQAILFAEAQVDAGADFIGIGDAAASLISPRMYRKWAMPYEKRI
                     IEAIHRKGAKAKLHICGNTSHFLSDLPQTGADMIDADWMVDFKTAVDTFGDQASACGN
                     FDPVSVMLNGTPETVRDAVNACVQVARPNTFIAAGCEVPKNTPVENLKMVAQTIQEAA
                     LNPTL"
     gene            complement(2002..>2639)
                     /locus_tag="PKV59_07170"
     CDS             complement(2002..>2639)
                     /locus_tag="PKV59_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HQP46569.1"
                     /translation="GIAENQAVVEKYADVLKDELNVKKVRLLDAASEAMEFTLNPQPK
                     QLGQKYGSLFPAIRKKILEMDSMTTGRQLLEGKSIQVEVDGKTYEILPEEVEVRAVAH
                     EGFVVASEGSNIAALVTTLTPELEREGLSREFVRRVQDLRKTADLDISDRIDVVYEAS
                     SELAEAIRENADYISNETLAVSLKEGPVPADWASVEDSFDGQTVKVGLRKA"
BASE COUNT          734 a          664 c          530 g          711 t
ORIGIN      
        1 tttgggtcgg ggaaaaatgc cgtcaatgcg gtcattgacg tcgatttcca aattcatcag
       61 ggcgaaataa tttctatcgt cggtacttcc ggctccggga aaaccaccct tgcgaaaatt
      121 ttactggggc tgttgaaaga aaccagcgga gaaatcctgt ataaagggaa accgcggctg
      181 ctgaaaagtc agaaagaacg tcgtgcatat tggcaaaata ttcaggcgat tttccaagat
      241 ccattttcta ccttcaacca gtttttccgg gttgataaaa ttttgaatga ctgtctgaaa
      301 ttgcagggat tggagcacat ttctcaaaaa gaaaaaacgg aaaaaatgca ggatgcctgc
      361 aaattcgtta atctgaaata tgaggaactt tataataaat atccgttcga actttccggc
      421 gggcaaatgc aacggctgat gattgcccga atatttatgg taaagcccaa tatcctgatc
      481 gctgatgagc cgacttctat gatcgatgct tgttcccgtt ccaccatcct ggatatgctg
      541 atgaaattgc gggaagaaga aggcatgacc atcctgttta taactcatga tttaggtctg
      601 gcatattatt tgagtgatac gatctatatt atggaatcag gccggattgt cgaaaaaggt
      661 cccgccgaag acgttatttt gcatccgcag gacagctata caaaacagtt ggtctcggat
      721 gttcccaagt tacatgaaga gtgggttctc agctactgag agcccacttt ccttctttcc
      781 cctttgaaat ttacaaggtc ggattcagag cggcttcctg aattgtttga gccaccattt
      841 tcagattttc gaccggtgta tttttgggta cttcacaacc tgcggcgata aaagtgttcg
      901 gccttgccac ctgaacacag gcatttacgg catcacggac tgtttcgggc gttccgttca
      961 gcataacaga aaccggatca aaatttccgc aggcagaagc ttgatcgcca aaagtatcca
     1021 ccgccgtttt aaaatcaacc atccaatccg catcaatcat atcggcaccc gtttgcggca
     1081 aatcggacag aaagtgggaa gtattcccgc aaatatgcaa ttttgctttc gcacctttcc
     1141 ggtgaatcgc ctcaataatc cgtttttcat acggcatcgc ccattttcga tacattcgcg
     1201 gactgatcag agaagcagcg gcatccccga tgccgataaa atccgcccct gcatcaacct
     1261 gtgcctctgc aaaaaggatt gcctgctgag tacagaattc aagcaattca gcggtaaatt
     1321 ccggatcatc taccacagag atcatgatat tactaaccga atccaaatca caagcttcgg
     1381 caaaagcgcc ttcaacccag cccattaccg gatattcctt ccccgctttt tctctgtaaa
     1441 gagaaacagc agctaatcga tcggacattc tgcgcccgtc ttccggtttg acgattttta
     1501 attttgccag atcggccgag caactgacga aaggaaccgt cggatgcgga acatcatcaa
     1561 ccggaaacgc gattgcggct cccaaatcgg atgcttccct taccggatcg gatattgcgg
     1621 agagcaaatc gattttaaaa tcttcgcaac aacgaatatt tccttcaact aaaagacgat
     1681 agtcttgcac aaattgatca tagggaacat gaatatattt tgcggcaaaa gtcatcaaaa
     1741 tacacaaatt cggaatccga tccaccggct tgccggccag tcgattcatc acccgctcat
     1801 aacagttcat aagattttcc tcccttgctt atcatacaca tccatggaaa aatttgcaat
     1861 cgatcacaaa aaatatatca ggaacaaatc cttttaagac tttcccgatt gaattgatcg
     1921 ggaactgtta cattcgatat tcgatcggaa aagaataagg aggggatttc acccctcctt
     1981 cgaacctgat cacaacattc tttatgcttt ccttaatcca accttgactg tttgtccgtc
     2041 gaaagaatct tcaaccgacg cccaatccgc gggaaccgga ccttctttta atgacacggc
     2101 cagcgtttca tttgaaatat aatccgcatt ttcccgaatg gcttccgcca gttcactgct
     2161 ggcctcatag actacatcga ttcgatcgga aatatccaaa tcagccgtct tgcgcagatc
     2221 ctgaacccgc cgcacaaatt ctctcgatag cccttctctt tccaactcgg gtgtcaaagt
     2281 tgtcaccaag gcggcgatat ttgacccttc cgaagcgacg acaaaacctt cgtgagcgac
     2341 ggctcgaact tccacttctt cgggaagaat ttcataagtc ttgccgtcca cttccacctg
     2401 tattgatttt ccttccagca actggcgacc ggtcgtcatc gaatccattt ccaaaatttt
     2461 ctttcggata gcgggaaata aactgccgta cttctgtccc aattgcttcg gttgtggatt
     2521 cagcgtaaat tccattgcct cggatgcagc atccaacaag cggacttttt taacattcaa
     2581 ctcatccttc aacacatcgg catatttttc gacaacagct tgattttccg ctataccta
//
LOCUS       DAOMIV010000300         2634 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_42620_length_2634_cov_3.881737, whole genome shotgun sequence.
ACCESSION   DAOMIV010000300 DAOMIV010000000
VERSION     DAOMIV010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42620_length_2634_cov_3.881737"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKV59_07175"
     CDS             complement(<1..345)
                     /locus_tag="PKV59_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQP46570.1"
                     /translation="MEIVEIKEMSQAVLDAFTVLVPQLSSSAKIPTWDELEDLIQSRA
                     SILLAAVDPDQNNKIVGSLTLAVFRLPTGVRSWIEDVVVDEQMRGRGVGEMLVRAAVD
                     RARAEGAKTVDLT"
     gene            587..2455
                     /locus_tag="PKV59_07180"
     CDS             587..2455
                     /locus_tag="PKV59_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46571.1"
                     /translation="MRQKSYKKDRRNGRGFSVCILILALICLIPVAGGYEFTLSAGEF
                     LAPDGTVESFTPEVYSWLRAVFSVLFLVLMGLFITSVCIPERFFRFWREQWRSFKGFP
                     SALKSDTGTFFSAVRQFFRENRKDIPLLLILFLSGMAIRLWIINRPITHDEAYTITTW
                     GSGSLKYAVSDYQLPNNHVFHTILVNLIYHTLGKTPALLRLPALINGLLLIPAVYILG
                     KQIYNSATGLLAAGISAFSSFLIDYSVWARGYSILAFCAVLIFIEGTIVQKQKNRFVW
                     CLLILTAVIGFYTLPVMLYPFGAFCVWFLLRALFHRKFPLSGYGSMANALKYFIAAGI
                     VIVILTGLCYLPILRNSGIHALFGNVFVRPLPLADFKPTLISRAIDFYRAFTDTVPPV
                     IRIALFLGNVLALIFHRKISRIFIPLQAAFLLWLLPLWAIQRPNLWPRTLIFLWPFLI
                     LWGSAGVIWIVEKICAFLPSGLQTRFIPLFIIVAAGVTAVPQFDRAAEIAKLSSPHET
                     AVLRILENQDTEKILFVVAPEDDAPLWYYADRYGLPKSIFDKNIPFSTVYVYVNPKNE
                     GFEEPRTLEEIINRYGPGESFLDRSTEKVLMASENAILYRFHANERVIRKTFGETP"
BASE COUNT          618 a          656 c          572 g          788 t
ORIGIN      
        1 ggttaaatcg accgtttttg caccttccgc tctggcacga tcgacagcgg ctctgaccaa
       61 catttcaccg acaccccgcc cgcgcatttg ctcatctacg actacatcct caatccacga
      121 tcggacgccg gtaggtaatc ggaaaacagc caacgtcaga ctacccacaa ttttgttatt
      181 ctgatccgga tccaccgcag ccagcaaaat ggatgcccgg gattgaatca gatcttccaa
      241 ctcgtcccag gtcggaattt ttgccgagga ggaaagctga ggaaccaaaa ccgtaaatgc
      301 gtctaaaacg gcttgagaca tttcttttat ttcaacgatt tccattttta tgctcttttc
      361 taaaaatgtc aaatttctgc tggttattat actatgtgaa aaaatggttt aaatcgttct
      421 ttagaagcct tttttttcgc aaaatattcc gaaatcggta aaaataatga taaaacagtt
      481 catagacaat ccgaaaagtt aatgtatcat taccgaaaga ttatgtcaga ccttacggag
      541 cgaatttcgc aaacaatgat aaccggttca gaaagagtgc atcccgatgc ggcaaaaatc
      601 atataaaaag gatcgacgaa acggcagggg gttttccgtt tgtattttaa ttctggcatt
      661 gatttgtctg atccccgtag cgggcggata tgaattcacg ctgtccgccg gtgaatttct
      721 tgcgccggac ggtacggttg aatcattcac ccccgaggtt tattcatggc tccgggcagt
      781 gttttcggtt ctctttcttg tcttaatggg acttttcatc acatccgtct gtattcccga
      841 acgatttttc agattttggc gggaacaatg gcgatctttc aaaggattcc cctcggcatt
      901 gaaaagtgac accggcacgt ttttcagcgc cgtccggcaa tttttcagag aaaacagaaa
      961 agacattccg ctgctgctga tcctctttct gtccggcatg gcgatccgct tgtggattat
     1021 caatcgtccg attactcacg atgaagctta taccatcacc acatggggaa gcggaagcct
     1081 gaaatatgcc gtcagtgatt atcagttacc gaacaatcat gtttttcaca cgatcttagt
     1141 aaatttaatc tatcataccc tcggtaaaac gccggcgctt ttacgactgc cagcattgat
     1201 caacgggttg ctgttgattc ccgccgttta tatcctcgga aagcagattt ataattcggc
     1261 gaccgggctg ctggcggccg gaatctccgc attttcgtct tttttgattg actattccgt
     1321 ttgggcgaga ggttattcca ttttagcctt ttgcgcggta ctgattttca tcgaaggaac
     1381 aatcgttcaa aaacaaaaga accgttttgt ctggtgcctc ttgattctca ccgcggtcat
     1441 cggattctat acgctaccgg ttatgctcta tcctttcgga gctttttgcg tttggttttt
     1501 gctccgagcc ctctttcatc ggaaatttcc gctttccggt tacggaagta tggcaaacgc
     1561 cttgaaatat ttcatcgcgg cgggtatcgt aattgtgatt ctgaccgggt tgtgttattt
     1621 gccgattttg cggaattccg gcattcacgc ccttttcgga aacgtatttg tccggccttt
     1681 gccgctcgcc gatttcaagc ccactctaat ttcgcgcgcc attgatttct accgcgcttt
     1741 caccgatacc gtcccacccg taatccggat tgctttgttc ctcggaaacg ttttagcttt
     1801 gatatttcat cgaaaaatca gtcggatctt cataccgctc caagcggcat ttctgctgtg
     1861 gctcttgccg ttatgggcaa tccagcgtcc caacctttgg ccgcgaacct taatcttcct
     1921 ctggccgttt ctgatcctgt ggggatctgc cggggtcatt tggattgtcg aaaagatatg
     1981 cgcattttta ccatcgggtc ttcaaacaag attcattccg ctttttatca tcgttgccgc
     2041 cggtgtcacc gctgttccgc aatttgatcg agccgctgaa attgcaaaat taagcagccc
     2101 gcatgagaca gccgttctca ggatcctgga aaatcaggac accgaaaaga tcttgtttgt
     2161 ggtcgcaccg gaagatgacg ccccgttatg gtattacgcc gatcgatatg gcttgccgaa
     2221 atcaattttc gataaaaata ttcctttttc gacggtctat gtctatgtca atccgaaaaa
     2281 cgaagggttc gaggaacctc gaacccttga agaaattata aatcgttacg gacccggcga
     2341 atccttcctc gaccgaagta ccgaaaaagt actgatggct tccgaaaatg ccatccttta
     2401 tcgctttcat gcgaacgagc gtgttattcg gaagactttc ggcgaaactc cctgacgatc
     2461 cgctgtcaac ctttccgttt cgtttgtttg ggttgccctt tcgtcagagc gggggcgcct
     2521 gacttcatcc gagcgctggc gatttctctt cgaaacaact tcttctccgg tttttttgcc
     2581 ggttttgcag aagcagattc tttcaatgcc tgaacttctt catctttcaa ggca
//
LOCUS       DAOMIV010000301         2633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_42643_length_2633_cov_3.564779, whole genome shotgun sequence.
ACCESSION   DAOMIV010000301 DAOMIV010000000
VERSION     DAOMIV010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42643_length_2633_cov_3.564779"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PKV59_07185"
     CDS             <1..1313
                     /locus_tag="PKV59_07185"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQP46572.1"
                     /translation="MRVAHSASPVEIGERNWDDEKIPEKWVRDQDKYVCSAMLAKKKR
                     PMIEDRLIKLVDVSNKISINRLEWGDRKLGIIASGPVYHYAREIFPNATFLKLGMTYP
                     LPEGLIRELAAGVDQVMVIEELDPFIEQQVKAMGINCIGKDIFPRYGELLTGDIERAC
                     AKAGIIPWEKVAEQRDVTPENLPGRSPVLCPGCPHRSTFYNLAKMKDVIIVSDIGCYN
                     LGALAPFYATDFMGSMGAGFGVAHGFEIGGIEKTGQKIIGIVGDSTFFHNGVTPLLNM
                     VHNGSNVTLMVLDNSTTAMTGHQDHPGVDYTLRGKGTRVDIEKMVRAIGVEHVLKANA
                     FDVKSVDSAIKEGLNHDGLSFIIVEGPCFFVGNNLKPAYTVTSNCNGCGTCFKLGCSA
                     IARSDIVDEKTGRRKSSIDPVLCVGCGICAQVCPRKAIVSTQAS"
     gene            1333..1923
                     /locus_tag="PKV59_07190"
     CDS             1333..1923
                     /locus_tag="PKV59_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HQP46573.1"
                     /translation="METINFLITGVGGQGTVLASEIMAAVGFAAGYDVKKSDLLGLSV
                     RGGAVLSHVRWGEIVYSPIVPEGRVDYLIAFEELEALRHLGQVKSTGTILMNQQKIFP
                     VLVSSGLAEYPTDEVIEESLRKTSKNVHEIPAVELALKVGNSKVLNIVLLGAISALFN
                     IDPKIWEEVIKERVPKKYVDLNIKAFNEGRNWMKGE"
     gene            1930..>2633
                     /locus_tag="PKV59_07195"
     CDS             1930..>2633
                     /locus_tag="PKV59_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HQP46574.1"
                     /translation="MRICVINPNSSKSVTDHLRNTLNKIKRDDCELTVICPEHGPVSI
                     ESAYDETIAAFYVMDLVKKANEDGYDAIVLACFSDPGLDAAKEISSIPVIGIEEATLH
                     MAAMLGHKFSIMTGMERRIPTRELHITIHGLSQSYASSPALEMSVLEMDSNPQKAKEK
                     IIELARKAVKEDRAEVIILGCAGLAGYSEDIERELGVVVLDPSSVALKVAEAMVDLGL
                     HHSKVGRFSFPPAKEI"
BASE COUNT          781 a          512 c          632 g          708 t
ORIGIN      
        1 gtatgcgcgt tgcccattcc gcttcaccgg ttgaaatcgg ggaaagaaat tgggatgatg
       61 aaaaaattcc cgaaaaatgg gtcagagatc aggataaata tgtttgttcg gcaatgttgg
      121 caaagaaaaa acgacccatg attgaagatc gcctgataaa gttggtggat gtttccaata
      181 aaatttccat taaccgtttg gaatggggag atcgaaaatt gggaataatc gcatccggtc
      241 cggtttatca ttatgcgcgt gaaatatttc cgaatgcgac ttttctaaaa ctcggaatga
      301 cctaccccct tcccgaaggt ttaatccgtg aattagcggc gggagtcgat caggttatgg
      361 tcatcgaaga attggatccc tttatcgaac aacaggtaaa agcaatgggg atcaattgca
      421 tcggaaagga tatattcccc cgatacggag aattattgac cggagatatt gaaagagcct
      481 gtgcgaaagc gggaattatt ccttgggaga aagtcgctga acaacgagat gtaactcccg
      541 aaaatcttcc cggaagatct ccggttttat gccccggttg tcctcatcga agtacatttt
      601 acaacctcgc caaaatgaag gatgtcatca ttgtcagcga catcggctgt tataacttgg
      661 gcgctttagc tcccttttac gctaccgatt ttatgggatc aatgggcgcc ggttttggcg
      721 ttgcacatgg tttcgaaatc ggcggaattg agaaaaccgg ccaaaaaatt atcggtatag
      781 tcggggattc gactttcttt cacaatggtg tgactccgtt gctgaacatg gttcacaacg
      841 gaagcaatgt cacattgatg gtattggata attcaaccac tgcaatgacc ggacatcaag
      901 atcatcccgg tgtcgattac acacttaggg gaaaaggaac acgggtagat atcgaaaaaa
      961 tggtcagagc aatcggagtt gagcacgtcc ttaaagcaaa tgcttttgac gtgaaaagtg
     1021 tggattcggc aataaaagaa ggcttaaatc atgatggttt atcctttatc atcgtcgaag
     1081 gcccctgctt ctttgtcgga aataatctga aacccgcata tacagtgact tcaaattgca
     1141 atggctgcgg tacctgtttc aaattaggtt gttctgcaat agcccgatcg gatatcgtgg
     1201 atgaaaaaac ggggagacga aaatcatcta ttgatcccgt cctttgtgtc ggatgcggaa
     1261 tctgcgctca agtttgtcca cggaaagcaa ttgtatcaac ccaagcctca taaccagaca
     1321 aggaaaaact ttatggaaac gatcaatttt ttaattacag gagtaggcgg acagggtact
     1381 gtcttagcaa gtgaaattat ggctgcggtc ggtttcgcag caggatatga cgtaaaaaaa
     1441 tccgatctgc ttggactttc ggttcgggga ggcgcggtac tcagccatgt tcgatggggt
     1501 gaaattgttt actcgccgat cgtaccggag ggacgggtgg attatctgat tgcattcgaa
     1561 gagctggaag cgctccgcca tctcggtcaa gtaaaatcaa ccggtacaat cctgatgaat
     1621 cagcagaaaa tatttcctgt gttggtttca agcggacttg ccgaatatcc gaccgatgag
     1681 gtcatcgaag aatctttgcg taaaacttcg aaaaatgttc atgaaattcc ggcagttgag
     1741 ttggcattga aagtcggaaa ttcaaaagta ttgaatattg ttcttctcgg agcaatatcc
     1801 gcacttttta atatcgaccc taagatatgg gaagaagtca taaaagaacg ggtccctaaa
     1861 aaatacgttg atttaaatat aaaagctttt aatgaaggtc gcaattggat gaaaggagaa
     1921 taaacaacca tgcgaatttg tgtaattaat cccaattctt ctaagagtgt gacggatcac
     1981 cttagaaata ccttaaataa gatcaaaaga gatgactgtg aactcaccgt gatctgccct
     2041 gaacatggac ccgtctcaat tgaatccgct tatgatgaga caatcgcagc tttttatgtt
     2101 atggatttag ttaaaaaagc gaatgaagat ggatatgatg ccatcgttct cgcctgcttt
     2161 tcagatcccg gtttggatgc cgcaaaagaa atttcttcaa tccctgtgat cggtattgaa
     2221 gaagcaaccc tgcatatggc agcgatgctc ggtcataaat tttcgattat gaccggaatg
     2281 gaaagacgaa ttccgacccg cgaattgcat atcactatac atggtttgtc tcaatcttat
     2341 gcctcttctc ctgctttgga aatgtcggtt cttgaaatgg atagcaatcc ccaaaaagca
     2401 aaggagaaaa tcattgaatt agcccgtaag gcggtaaaag aagatcgggc agaggtgata
     2461 attctcggtt gtgccggatt ggctggatat tcggaagata tcgaacgtga attaggagtg
     2521 gttgttctcg atccgagttc tgtcgcactt aaagttgcgg aagcaatggt agatcttggg
     2581 ttgcatcata gcaaagtcgg acgcttttca tttcctccgg ctaaagaaat aaa
//
LOCUS       DAOMIV010000302         2614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_43115_length_2614_cov_4.723720, whole genome shotgun sequence.
ACCESSION   DAOMIV010000302 DAOMIV010000000
VERSION     DAOMIV010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43115_length_2614_cov_4.723720"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /gene="dinB"
                     /locus_tag="PKV59_07200"
     CDS             <1..1319
                     /gene="dinB"
                     /locus_tag="PKV59_07200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQP46575.1"
                     /translation="RLMMSRRQRLFRLPQFSEKTDKIVFPLFYSSIIIEQMAKYRKIL
                     HLDLDAFFCAVEELLNPSLKGKKFAVGGSPDRRGVVSTCSYAARKCGIHSAMPMSQAI
                     RRCPDLIIVHSGFEYYREKSREVMAILQHFTPLVEQISIDEAFLDVTDLPQPIEEIAR
                     SVQKEINQKPHLPCSLGAASNKLVAKIADNIGKKRVITDTYPNAILWIPNGKEADFLS
                     PLKVDELWGVGKKTAQIFENLGISTIGQLAAYPVSLLVPYLGKSAAEYHDRALGIDDS
                     PVEPEPAAAKSISQETTFERDVSDETILRETIRRQSDQVGFRLRSQKLSGNTVKIKLR
                     WSDFTTITRQTKSEIALSQNSLINKAAQELFDSVWLVNPRPVRLIGVGVSGIEKDVRQ
                     LSFFSADTEKEDHFLQAVDQIRNKYGDQALTRAAEISRKPRNLKNK"
     gene            1436..1508
                     /locus_tag="PKV59_07205"
     tRNA            1436..1508
                     /locus_tag="PKV59_07205"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1469..1471,aa:Thr,seq:cgt)
     gene            complement(1615..2382)
                     /locus_tag="PKV59_07210"
     CDS             complement(1615..2382)
                     /locus_tag="PKV59_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HQP46576.1"
                     /translation="MAKRRALGIDVSHWQGNVDFKKAKKAGASFAIIKASQNVWYDDK
                     FQQNWANAKDAKIIRGAYHFFDMRDGSKSPKEQARYFAGLLKEDMGELRPVMDFESPG
                     VNGYPQLPDHDTCVRHVVDFMNAFYKETNLYPMLYTNLAGITRLSPLSRFLSAKELWI
                     AWYYTKSNQPKIGDWPRWRIWQYKVTGNGLAFGAESSGLDINAFNGTEEDLYKYANAL
                     GISPTPLTMNKSTDDFLPDSLKGDLADDLKDPQLKIE"
BASE COUNT          754 a          557 c          570 g          733 t
ORIGIN      
        1 cccgtttgat gatgagccgc agacagcgat tatttcggct gccgcaattt tccgaaaaaa
       61 ccgataaaat agttttcccc ttgttctatt cgagcattat aatagaacaa atggcaaaat
      121 accgaaaaat acttcactta gatttggatg cttttttctg tgcggtcgaa gagttgctca
      181 atccttcttt aaaaggaaaa aagtttgcgg ttgggggcag tccggatcga cggggagtcg
      241 tctccacctg ttcctatgca gccaggaaat gcggcattca ttccgcaatg ccgatgtccc
      301 aagcgatccg acgctgtcca gatttaatca ttgttcacag cggatttgag tattatcgtg
      361 agaaatcccg tgaagtgatg gcaatccttc agcattttac gccgttggtt gaacagatat
      421 cgattgatga agcctttctg gatgttacgg atcttccgca accgatcgag gagattgcgc
      481 ggtcggtgca aaaggaaatt aatcagaagc cgcatttgcc ctgttcgctc ggagccgcat
      541 ccaataagct ggttgctaaa attgccgata acatcggaaa aaaacgggtt atcacggata
      601 cctatcccaa tgcgattctt tggattccga acggaaaaga agccgatttt ttatcacctt
      661 tgaaggtgga tgaactgtgg ggtgtcggga agaaaacagc ccaaattttc gaaaatttag
      721 gcatatctac gatcggacaa cttgccgctt atcccgtatc tttattagta ccttatttgg
      781 gaaaatcggc ggcggaatat catgatcgtg ctctgggaat cgatgacagt ccggttgaac
      841 ccgaacccgc tgccgccaaa tcgatcagtc aagagaccac ttttgaaaga gatgtctcgg
      901 atgaaacgat tctgagagag acgattcggc gccaatcgga tcaggtagga tttcggttac
      961 gcagccaaaa attatcgggc aatacggtga aaattaagtt gcgctggtct gatttcacca
     1021 caatcacgcg gcaaacgaaa agtgagattg ccctttcaca aaattcattg atcaataaag
     1081 cagcccagga attattcgat tctgtttggt tagtcaaccc gcgaccggtg aggttgatag
     1141 gagtcggagt aagcgggata gaaaaagatg tcagacaact atcgtttttt agcgccgata
     1201 cagaaaaaga agatcatttt ttgcaagctg ttgaccaaat tcggaataaa tatggagatc
     1261 aagcattaac ccgggctgcc gaaatttcgc gaaaaccgcg gaatctaaaa aataaataag
     1321 attgaaacac ttttgcggat aagttctgac aatatcttta ttttatttga acggatctgc
     1381 ctgaaatgtc aaaattattg ttcggaaact ggtataatac cccttcagaa aaagtgccaa
     1441 catagctcag ccggtagagc aggcgtttcg taaacgtcag gtcaggagtt cgaatctcct
     1501 tgttggctct tttttttatt aaaagttcta cgactttatt taaaatgaaa cgcctccgga
     1561 aaggggcgtt tcatttgcaa tggtaatact tgtttcaaaa aacaagagga agaattattc
     1621 gattttgagt tgtggatcct tcaggtcatc cgccagatca cccttcaggc tatcgggcag
     1681 aaaatcgtcg gtggatttat tcatcgtcaa cggggtgggc gagataccca gagcgttagc
     1741 gtatttatat aaatcttctt cggtgccatt aaaagcatta atatccaatc cgctggattc
     1801 ggcaccgaat gccaaaccgt ttccggtcac tttatattgc caaatccgcc aacgaggcca
     1861 atcaccgatc ttgggttgat tggattttgt ataataccaa gcgatccaaa gctcctttgc
     1921 ggacaggaat cgactcagag gagaaaggcg ggtaattccg gcaagattcg tatataacat
     1981 cggatacaga ttggtttcct tgtaaaaagc attcataaag tcaacaacat gtcgaacaca
     2041 ggtatcatgg tcaggcaact gaggataacc attcactcca ggagattcaa aatccattac
     2101 cggtcgcagc tctcccatat cttctttcaa aaggccggca aaatatcttg cttgttcttt
     2161 gggagatttg gatccgtccc gcatatcaaa aaaatgataa gcgccccgga ttatttttgc
     2221 atctttggca tttgcccaat tctgttggaa cttgtcatcg taccatacgt tttggctggc
     2281 tttaataatc gcaaaagatg ctccggcttt tttggctttt ttaaaatcga cattcccttg
     2341 ccaatggctg acatcgatcc ctaatgctct gcgtttcgcc acgcgccctc ccttccgaac
     2401 gatcttgctt ctgtcgcttt atctgcttta aactaagttg caaaaagtgt gccaaagaaa
     2461 gataaaaagt attttccgat ttcaatcgtt ttttagaaac aacgccattg atataccaaa
     2521 aaagagccga acaagaaatg gctctttttt gatttgaaga ttccgaaaag acgacttaat
     2581 ttattgtcag tttaatcttc atcttcctca aatt
//
LOCUS       DAOMIV010000303         2612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_43154_length_2612_cov_4.879155, whole genome shotgun sequence.
ACCESSION   DAOMIV010000303 DAOMIV010000000
VERSION     DAOMIV010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43154_length_2612_cov_4.879155"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="PKV59_07215"
     CDS             <1..273
                     /locus_tag="PKV59_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46577.1"
                     /translation="VAWAFGGDFVYQGGTSYLSGTANVFIDQNGKAKAVYGGSFATDP
                     GSRAEVNQTKVEVRGIAETMTECGEHSKDGISIVRSKIDCTVNSVP"
     gene            463..1146
                     /locus_tag="PKV59_07220"
     CDS             463..1146
                     /locus_tag="PKV59_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HQP46578.1"
                     /translation="MEYKIEGAPFPVLICTLSNGEVMVTESGAMCWMTPNITMEATSR
                     GGLGKALGRMLSHESLFLNTFTASGTGMVAFGSSFPGMILPFDIEPGKELIVQKSGFL
                     ASEVGVELSMFFQKKLSSGLFGGEGFIMQRLSGQGKAFLEIDGYCKTYELGPGEKMLI
                     DTGYLAAMEPTCAMDIKKVSGGLKTMAFGGEGLFNTEVTGPGKVYLQTSPIKTLAGAL
                     LPYIPSKSS"
     gene            1383..>2612
                     /locus_tag="PKV59_07225"
     CDS             1383..>2612
                     /locus_tag="PKV59_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46579.1"
                     /translation="MKDYMSLQNGSDIRGIALDGDNGEKANLTSEAVFDITKSFVFWL
                     SEKAEKPSKILKIGVGTDSRLSADIMRQAILSGIEAGGANGFDCGMTSTPAMFMSTVF
                     PESDFDGAIMATASHLPWNRNGMKFFTKGKGTESADVTAILTKAQETLPEVPSGTRTK
                     FDLMPLYNSHLQTVIRNGIEDQSEFPLAGYKIAIDAGNGSAGFFATEVLEPLGADISG
                     SQFLEPDGRFPNHVPNPELPEVMEAAKKMTLESHSDLGIVFDTDVDRAAFVDDKGTIL
                     SRNRLIGVLAEIVSKKYPGCTIVTDSVTSDHLTKFISKLGCSHHRFKRGYRNVINEAI
                     RLNREGIICPLAIETSGHCAFSDNYFLDDGAYLAAVMIAETVKCRREGRSLADLASEL
                     KDPLESVEYRIKILNPDP"
BASE COUNT          741 a          529 c          641 g          701 t
ORIGIN      
        1 gtcgcctggg cttttggcgg agatttcgtt taccagggcg gtacatctta tttatccgga
       61 acagccaatg tcttcatcga tcaaaatggg aaagcaaaag cggtttacgg aggaagtttt
      121 gccacagatc ccggcagcag agccgaagtg aatcaaacaa aagtcgaagt caggggtatt
      181 gccgaaacaa tgacggaatg tggagaacat tccaaagacg gaatctcaat cgtccggtca
      241 aaaatcgact gtacggtcaa ttccgttccc tgatttttca atctgcaaag atttttatcg
      301 gaattgggat ttccaaatct cagccggttt tccggtcgtc caagccttct atatgactta
      361 ttcatagtgg aacgctgcag cccaaaaata tccttgacac tataagaatt cagcatatac
      421 tgaaaagtgg caaaacaacc aatccagaaa tgagagggta atatggaata taaaattgaa
      481 ggggctccct tcccggtatt aatctgtacc ttgtcaaacg gtgaagttat ggtgactgaa
      541 agcggtgcta tgtgctggat gactccgaac atcaccatgg aagccacctc aagaggtggt
      601 ttgggcaaag cgttgggcag aatgttatcc cacgaatcat tatttctgaa tacgtttacc
      661 gcaagcggaa ccggcatggt tgcattcgga tcttccttcc ccggcatgat ccttccgttt
      721 gatatcgaac ccgggaagga attgattgtt caaaaatcag gctttttggc atcggaagtc
      781 ggggtcgaat tgtcaatgtt ctttcaaaag aaattatcca gtgggctttt cggcggtgag
      841 ggttttatta tgcaacgcct tagcggtcaa ggaaaggctt ttcttgaaat cgacggatat
      901 tgcaagacgt atgagctggg ccccggagaa aaaatgttga ttgacaccgg ttatctcgcg
      961 gcaatggaac caacctgtgc aatggatatt aaaaaggtca gcggcggatt gaaaaccatg
     1021 gctttcggcg gagaaggatt atttaatacg gaagtaaccg ggccgggaaa agtatacttg
     1081 cagacatctc cgattaagac gcttgcgggt gctctccttc cctatattcc ttcaaagagc
     1141 agttagatga ttgatttaac ggaatcgatt cctcagacgg ttccaaacaa ttgaatattt
     1201 tgtatttcct aaacggatgc ctgaagtcgg gcgtccgttt ttttgtacgg aaattatcat
     1261 gagtgataat tccaaaaagg aaaacatatt cgaatgaatt ttccgatgga cattgattct
     1321 ttgatgacaa atgttataaa ataataaagg ttcattcaac aaatttaaaa tgaggtttcg
     1381 aaatgaaaga ttacatgtcc cttcaaaacg gcagtgacat cagagggatt gcgctcgatg
     1441 gagacaacgg agaaaaagcg aatcttacgt ctgaagcggt ttttgacatt acgaaaagtt
     1501 tcgttttttg gctttcggaa aaagcggaga aaccttccaa aatcttaaaa atcggagtgg
     1561 ggaccgattc ccgattgtct gccgatatca tgcggcaagc gattttatcc ggtatagaag
     1621 ccggcggtgc aaacggattt gattgcggaa tgacatcgac gccggctatg tttatgtcga
     1681 ccgtctttcc tgaatccgat tttgatggtg ccatcatggc tacagccagt catctgccgt
     1741 ggaatcgaaa tgggatgaaa ttctttacca aaggcaaagg aacggaatcg gcggatgtga
     1801 ccgctatttt aaccaaagcc caggaaacgt tgccggaagt tccttccgga acacgaacaa
     1861 aatttgactt gatgcctctt tacaattctc atttacagac ggttatcaga aatgggattg
     1921 aggatcaaag tgaatttcct ttagccggat ataaaatagc gatcgatgcc ggaaacggtt
     1981 cggcaggctt ttttgccacc gaggttttgg aaccgttggg agcggatatc tccgggagtc
     2041 aatttttgga accggacggc cgtttcccga accatgtgcc caatcccgag ttgcccgaag
     2101 tgatggaagc ggcaaaaaaa atgacccttg aaagtcacag tgatctcggg atcgtttttg
     2161 atacggatgt cgatcgggcg gcttttgtgg atgataaggg aacaatcctc agccgtaatc
     2221 gtctgattgg ggttttggcg gaaattgttt ccaaaaaata tcccggttgc acaattgtga
     2281 ccgattccgt cacatcggat catttgacaa agtttatatc caaattggga tgtagccatc
     2341 atcgctttaa acgcgggtat cgaaatgtaa ttaatgaagc cattcgctta aacagagaag
     2401 gaatcatctg tccgctggct atcgaaactt ccggccattg tgctttttcc gataattatt
     2461 ttctggatga cggtgcgtat cttgccgctg tgatgatcgc cgagacggta aaatgtcggc
     2521 gggaaggaag atcgttggcc gatttggctt ccgaattaaa ggatccctta gagagcgttg
     2581 aatatcggat caaaattcta aatcccgatc cc
//
LOCUS       DAOMIV010000304         2611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_43193_length_2611_cov_3.627152, whole genome shotgun sequence.
ACCESSION   DAOMIV010000304 DAOMIV010000000
VERSION     DAOMIV010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43193_length_2611_cov_3.627152"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PKV59_07230"
     CDS             complement(<1..1178)
                     /locus_tag="PKV59_07230"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQP46580.1"
                     /translation="METGESKKLLSIRGITKAFGQNLVLKGVDMDLCGGEILALIGGN
                     GAGKSTLMKIIMGIYQPDAGCIFINGKEVQIINPSVALEECVYMVPQEPMLFPNMSVF
                     ENIIMGFHESNATLKARLQEIMADIGWQMDLTRKAITLSIAEQTLVEILRGLMRNAKI
                     LILDEPTSALTFDEVESFFRVVKELKNKGIGIIYITHRLNEVFEIASHVMIMRDGVIT
                     LGGPVEKFTREMLVRGLLPVDAEHKVENGSVHATIDYEHLEPAFELKDFCGYGFNDIS
                     LKVYPGEVLGVAGVVGAGRTELVSTIFGRDKSLGGKVYLDGKDITGFSTARVLEAGIN
                     YVPEDRHYHGLFCISTIAANTTSALLPSPEMGNFILDTKNEYSITQRFIDLFRIKVTD
                     "
     gene            1571..2503
                     /locus_tag="PKV59_07235"
     CDS             1571..2503
                     /locus_tag="PKV59_07235"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HQP46581.1"
                     /translation="MELEEDLMSKIAWYYYIDGLTQQQIADLIGIPRLRVIRLLDKAR
                     QTGIIQFKIRKDNSNTELEHELARRFGLKDVFVVPSGSEKIGDRNEAIAQAAAMYISD
                     RLPDNAFINMGYGDTSSKILNHLANKQGQRINVVSLTGGVNYYLPNTQSNIFSAKLYL
                     TPAPFIMSTKEMVQAINNEPAVREIAEMVPLSTMSVVGIGAMNENATVIRNGIFTKKE
                     LLYLTMQGAVGDVLCHYFDINGKQIASEVEDRLITTSLETLAKQDNVIGVAAGPQKVN
                     AIIGALNGKFLDILITESEIAETILEKTQQLAVP"
BASE COUNT          795 a          556 c          523 g          737 t
ORIGIN      
        1 tggtcggtaa ctttaatccg gaaaagatca ataaaccttt gcgttatgga atattcattt
       61 tttgtgtcca agataaaatt tcccatttca ggagatggga gaagggcgga agttgtattc
      121 gcggcgattg tgctgatgca aaagagtccg tgataatgac gatcttccgg gacataattg
      181 attccggctt ccagaactct tgcggttgaa aagccggtta tgtccttccc atcaagatag
      241 acttttcctc caagagattt atcacgccca aaaatggtac tgacaagttc cgttcgtccg
      301 gcaccgacga caccggcgac acccagcact tcaccgggat aaaccttcag cgaaatatca
      361 ttaaatccgt aaccgcaaaa atcttttaat tcaaatgccg gttccaaatg ttcataatcg
      421 atagtggcat gtacggaacc gttttcaacc ttatgttcgg catccactgg taaaagtccg
      481 cgtacaagca tttcacgggt gaatttttcc accggccctc caagtgttat cactccgtcg
      541 cgcatgatca tgacatgaga agcgatttca aaaacttcat tcaaacggtg ggtaatataa
      601 ataattccga tccctttatt cttcagttct tttaccacac ggaagaagga ctctacctca
      661 tcaaatgtta aggcggaagt cggctcgtca agaatcagga tttttgcatt ccgcatcaac
      721 ccacgtagaa tttcaaccaa cgtctgttcc gcaatggaaa gcgtaatcgc ctttcgggtt
      781 aaatccatct gccatccgat atccgccata atctcttgaa ggcgagcctt caaggttgca
      841 ttggattcgt gaaagcccat aatgatattt tcaaaaacgc tcatgttggg aaacagcatc
      901 ggctcttggg gaaccatata aacacattcc tctaaagcga cggaagggtt aataatctga
      961 acttcttttc cattaatgaa aatacatccg gcatccggtt gataaatgcc cataataatc
     1021 ttcattaagg tgctctttcc ggcaccgttt ccgccgatta atgccaatat ttccccgccg
     1081 caaaggtcca tatcaacacc ttttagcaca aggttctgtc cgaaagcttt ggttattcct
     1141 ctgatcgaca gtagtttttt ggattcaccc gtttccataa atacggtgct cctattttca
     1201 aagaatcatt ctctcagata aatacttttg tgtagcaacc atacaataag tatgtaacta
     1261 agataccagt attaaccttg tttgtcaata gttaattcga cgctattttt catgacgatt
     1321 cataaaagtc aaaatttatc tcgtatttat gagccccgtc tttgattttt ccctaagttt
     1381 tacaataacc attgacaagc ttttttctct tcattataat aaccaaaagt gaacatttat
     1441 ttacaatacg atacaaatgt ttatttattg aaagacgatc aaacactgta atccgtattg
     1501 gcgttgagac cgagtttttt tgtaaacagg aaaaagtttt gtacataatt gaaatgaaag
     1561 gcaatttaat atggaactag aagaagacct gatgtcgaaa attgcttggt attactatat
     1621 cgatggtctc acccagcaac agatcgccga tctgatcggg attccaagat taagagtcat
     1681 tcgtctgtta gataaagctc gtcaaacggg aattatccag ttcaaaattc gtaaagataa
     1741 ttcaaatacc gaattggaac atgaactcgc cagacgattc ggattgaaag atgtcttcgt
     1801 tgttccatcg ggttcggaaa aaataggtga cagaaatgaa gcaatcgcac aggcagctgc
     1861 aatgtatatc agtgatcgcc tgccggataa cgcttttata aatatggggt acggagacac
     1921 ttcaagtaag atccttaacc atcttgccaa caaacaagga caaagaatca acgtcgtttc
     1981 gttgacagga ggggtaaatt attatttgcc caatacccaa tccaatattt tcagtgcaaa
     2041 actctatctg acgcccgctc cgtttattat gtcaacaaaa gaaatggttc aagccataaa
     2101 taatgaacct gctgtccgtg aaattgcgga aatggtcccg ctctcgacaa tgtcggtggt
     2161 cggtatcggc gcgatgaatg aaaatgccac cgtaatacgg aacggtattt ttacaaaaaa
     2221 agaattactt tatctcacga tgcaaggggc ggtcggtgat gttttatgtc attacttcga
     2281 tatcaacggg aaacaaattg cttcagaggt cgaagatcgt ttgatcacaa cttctttgga
     2341 aacattggca aaacaggata atgttatcgg cgtggctgcc ggtccgcaaa aagtaaatgc
     2401 catcatcggt gctttaaacg ggaaattcct cgatattctg atcaccgaaa gcgagatcgc
     2461 cgagacaatt cttgaaaaaa cgcaacaatt ggccgttcca taacaataaa ggaggcaaaa
     2521 ggtgacaaaa aaatatctta tggcagtgga tgcgggtacc ggaagcgtac gggcggtttt
     2581 gtttgacctt gacggtcaac agatcggctg t
//
LOCUS       DAOMIV010000305         2607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_43281_length_2607_cov_3.934169, whole genome shotgun sequence.
ACCESSION   DAOMIV010000305 DAOMIV010000000
VERSION     DAOMIV010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43281_length_2607_cov_3.934169"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="amrA"
                     /locus_tag="PKV59_07240"
     CDS             <1..518
                     /gene="amrA"
                     /locus_tag="PKV59_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQP46582.1"
                     /translation="NGEDPYVALAKASLTTYLKTGKYMKVPSGLPAEMLNRRAGTFVS
                     LKKNGELRGCIGTILPVRSSVAEEIIHNAVSAGVEDPRFPPVTLNELDDLDFSVDVLS
                     EPEDISSPDQLDVKRYGVIVSSGYRRGLLLPDLEGVDTVEEQIDIARRKAGIPRGEPI
                     RLQRFEVVRHT"
     gene            515..1339
                     /gene="amrS"
                     /locus_tag="PKV59_07245"
     CDS             515..1339
                     /gene="amrS"
                     /locus_tag="PKV59_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQP46583.1"
                     /translation="MKATCEICFHHCSLAEGQIGFCGARKNVDGKVICDNYGKITAIA
                     LDPIEKKPLKHFFPGSYILSIGSYGCNLHCPFCQNSDISMQYAGIRTHFISPQELVNK
                     ALELKSDGNIGVAYTYNEPFISYEYVTDCAGLVHQNEMKNVMVTNGTVEEAPLRKILP
                     LIDAMNIDLKGFTGEYYRKLSGNLEVVKRTIEIAAKECHIEVTTLIVPGENDSDEEME
                     ALAGWLASVDPEIPYHVSRFFPHYHMPDKPPTPVKTVYHLAEIARKHLKYVYTGNC"
     gene            complement(1412..1903)
                     /locus_tag="PKV59_07250"
     CDS             complement(1412..1903)
                     /locus_tag="PKV59_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQP46584.1"
                     /translation="MESKTMQDLMAGFAGESQANRKYTAYARKAKAEGKNNAAKLFQA
                     AADAETIHASRHFEVAGKVGSTADNLKDAIAGETYEYTEMYPEFLKDAEAEGNKAAVG
                     TFTLAKKAEEVHAKLYKEVLDNLDETEEVFYYLCPVCGNIEKVRPGKCPICGADGSQF
                     IKY"
     gene            complement(1914..2363)
                     /locus_tag="PKV59_07255"
     CDS             complement(1914..2363)
                     /locus_tag="PKV59_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQP46585.1"
                     /translation="METEPAYRDLVKELKEKKIRLSYQRVKILDYLNRNRTHPTADEI
                     YTGLIAEIPSLSKTTVYNSLNSLVKANLVQELTIEDNENRYDIVTETHGHFKCEKCGK
                     IYDFKVGLDSFNPEELKGFQINQKGVYFKGICKKCLETNQKIQQNEE"
     gene            complement(2424..>2607)
                     /locus_tag="PKV59_07260"
     CDS             complement(2424..>2607)
                     /locus_tag="PKV59_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46586.1"
                     /translation="TGSNRVRNIIQADGEALCDEAGLWRNTFESFGSLFCLIISKFDD
                     FVTEFSWNEGLKDIQF"
BASE COUNT          713 a          606 c          537 g          751 t
ORIGIN      
        1 acaatggaga agatccatac gtcgcccttg caaaagcatc tctgaccact tatctgaaga
       61 cgggaaaata catgaaagtt ccttccggat tgccggccga aatgttaaat cgccgagccg
      121 gaacttttgt ttctctgaaa aaaaatgggg agctgcgagg ctgtatcggt acgattctgc
      181 cggtacgatc ttccgttgcc gaagagatca ttcataatgc ggtatctgca ggggtcgagg
      241 atccccgatt tccgccggtt accctcaacg aactggatga tcttgatttc agcgtagacg
      301 tcctttccga acccgaagac atttcttcac cggatcaact ggatgtaaaa cgttacggtg
      361 tgattgtttc gagcggttat cggcgcggat tgctgttacc ggatctggaa ggggtagaca
      421 ccgtcgagga gcaaatcgat atcgccagac gaaaagccgg cattccgaga ggggaaccga
      481 ttcgtctaca gcgatttgaa gtagtgaggc atacatgaaa gcaacctgtg aaatttgttt
      541 tcatcattgt tcgcttgccg agggccagat cggtttttgc ggcgcgcgga aaaacgtcga
      601 cggaaaagtt atctgtgata actacgggaa aattaccgcc attgcccttg atccgattga
      661 gaaaaagccc ctgaaacact ttttccccgg cagctatatt ctctctatcg gcagctatgg
      721 ttgtaattta cattgcccct tctgtcaaaa cagcgatatt tccatgcaat acgcagggat
      781 ccgcactcat ttcatttctc cgcaagaatt ggtgaacaaa gcgctggagc tgaaatccga
      841 cgggaacatc ggagtggctt atacctataa cgagcctttt atcagttatg aatacgtgac
      901 ggattgcgcc gggttagtac atcaaaacga gatgaaaaac gtaatggtta cgaacggaac
      961 cgtcgaagag gctcctttaa gaaaaattct gcctctgatc gatgccatga atatcgatct
     1021 caagggattt accggtgaat attatcgaaa attaagcggc aatttggaag tggtcaagag
     1081 aacaatcgaa attgcagcca aagaatgcca tatcgaagtc actacgttga tcgttcccgg
     1141 ggaaaatgac agcgatgagg aaatggaagc gcttgccggt tggctggctt cggtcgatcc
     1201 ggaaattccg tatcatgtct ctcgtttttt cccgcattac cacatgccgg ataaaccccc
     1261 gacaccggtg aaaacagttt atcatctggc ggaaatagcc cgtaaacatt tgaaatatgt
     1321 ctatacgggg aattgttagc ggatccgaca ataaagaaag gggagaaaaa taatcgaaat
     1381 tttcctcccc tttcttttca ttttaaaaag atcaatattt tatgaattga gatccgtccg
     1441 caccgcaaat cgggcatttt cccggacgga ctttttcgat attgccgcaa accgggcaaa
     1501 gatagtagaa gacttcttcg gtttcatcca gattatccaa aacttcttta tagagttttg
     1561 catggacttc ctctgccttt ttcgccagcg taaaggttcc aaccgcagcc ttattcccct
     1621 ccgcctcggc atcctttaag aattccggat acatttccgt atattcatac gtttccccgg
     1681 cgatcgcatc tttcagatta tcagcagtac taccgacctt tccggcaact tcaaaatgtc
     1741 gtgaagcatg aattgtttcg gcatctgccg ccgcttgaaa caatttggcg gcattatttt
     1801 ttccttccgc ctttgcttta cgagcataag cggtatattt acgatttgcc tgagattcgc
     1861 ctgcgaatcc ggccatcaga tcctgcattg ttttactttc catatgattc tccttattct
     1921 tcattttgtt gaattttttg attcgtttca aggcattttt tacagatccc tttgaaatag
     1981 acaccttttt gattgatttg aaaacctttt aattcttccg gattaaatga atcaagcccg
     2041 accttaaaat catatatctt tccgcatttt tcgcatttga aatgcccatg cgtttcggtg
     2101 acaatgtcat aacgattttc attgtcctcg atggtcagct cttgaactaa attcgccttg
     2161 accaaagaat tcaaagagtt ataaactgtg gtcttagaca gtgacgggat ttcagcgatc
     2221 aaccctgtat agatttcgtc ggcagtgggg tgagtacgat tccgatttaa ataatccaag
     2281 atcttgaccc gttgataaga caagcgaatc tttttctcct tcaattcttt taccaaatcc
     2341 cgatatgccg gctcagtttc cacagaatct cctcacttat aatataacta ttcctaattt
     2401 gtaatcatta caccttatta tactcaaaac tgaatatctt tcaagccctc attccaagaa
     2461 aattcggtga caaaatcatc aaattttgaa ataatcaagc aaaataagct tccgaaggat
     2521 tcaaaagtat tccgccatag tcccgcttca tcacaaagag cctccccatc cgcttgaata
     2581 atgttcctca cccgattgct acctgtt
//
LOCUS       DAOMIV010000306         2606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_43311_length_2606_cov_3.335947, whole genome shotgun sequence.
ACCESSION   DAOMIV010000306 DAOMIV010000000
VERSION     DAOMIV010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43311_length_2606_cov_3.335947"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PKV59_07265"
     CDS             <1..1357
                     /locus_tag="PKV59_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46587.1"
                     /translation="MMYQMLLRMFIRAPLMMICATFMALTINKELAVVFLVAIPVLAF
                     FFAFITLKAHPRFVKMLAKVDSLNKSVQENLIAIRVVKAFVQRDYENAKFESAALDLQ
                     NAQRQAEKIVILNNPIMTLTMDACIIAILWFGGNKIIIGEMMPGALFSFLTYTTQIIT
                     ALMLVSMVFTMFVISQASADRIVEVLEEKIDLTDDNGNPDLRVKEGEIEFRDVNFSYA
                     KSPDNLTLCDINLHIKPGEMIGIIGGTGSAKTTLVQLIPRLYDVLSGQVLVSGHDVRE
                     YKLDHLRDAVAMVLQKNVLFSGTIADNLRWGNENATDEELVRVCKVAQAHDFIMSFPD
                     GYQTVLGQGGVNVSGGQKQRLCIARALLKNPKIIILDDSTSAIDTATELRFRKAFREN
                     YRETTILMIAQRVQSIKDSDRILVMNEGKIDAFGTHDELIETNTIYREVFESQQKGVA
                     L"
     gene            1354..>2606
                     /locus_tag="PKV59_07270"
     CDS             1354..>2606
                     /locus_tag="PKV59_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46588.1"
                     /translation="MNSEKKNPNRSITRTPGPGKNKPRLERPKDIRGTLIRILKTMSD
                     YKLQLGIVAVLMAVSSLSSVLGTYMLRPIINDYIIPLIAQSSPDLSGLIGMLLKMAGI
                     YSAGVIGNYTASRLMVNISSGTLRKIRVGMFEHIQNLPIKYFDTRTHGEIMSHFTNDA
                     DTLREMISNSIPNLISSAIMITGVIVMMIILSWQLFLLVILQLILIFFTIKFIGSRSS
                     SYYIKQQRELGSLNGYIEEMIEGQKVVKVFTHENKVKKIFNQKSEDLFKVASLANMFS
                     LVIQPIFGSYSYIHYALTAAVGTIMAIHGMLDIGTIGAFLQYTRTFTQPINQMSQQIN
                     AILAALAGAERIFILMDEPVEADEGNIVLVNVEKDHNEVIVESPVHTGYWAWKDVDEK
                     GTVTYTPLKGDMRFENVSFAYEPGNP"
BASE COUNT          730 a          559 c          600 g          717 t
ORIGIN      
        1 gatgatgtat caaatgctct tgcggatgtt catccgggct ccgttgatga tgatctgcgc
       61 cacttttatg gcgcttacaa tcaacaagga attggcggtt gttttcttgg tggcgattcc
      121 ggtattggca tttttcttcg catttatcac tctcaaagcg catccgcgtt ttgtaaaaat
      181 gcttgccaaa gtggacagtc tcaataaatc cgtccaggaa aatctgatcg ctattcgggt
      241 tgttaaagct tttgttcaac gggattatga aaatgccaaa ttcgaatcag cggcgcttga
      301 tcttcagaat gctcagagac aagcggaaaa aatcgttatt ctcaacaacc ccattatgac
      361 cctaacgatg gatgcctgta ttatcgctat tctctggttc ggtggaaata agatcatcat
      421 cggtgagatg atgccgggtg cattgttcag ctttctaacc tataccactc aaattatcac
      481 cgctttaatg ttggtttcaa tggttttcac gatgttcgtt atttctcaag cctccgccga
      541 ccggattgtt gaagtgttag aagaaaaaat cgacctgacg gatgataacg gcaatccgga
      601 tctgcgagtc aaggaaggtg aaattgagtt ccgggatgtt aacttcagtt atgctaagag
      661 tccggataat ctgactttat gtgacattaa ccttcatatc aaaccggggg agatgatcgg
      721 aattatcgga gggacgggct cagcaaaaac gaccttggta caacttatcc cgaggttata
      781 tgatgttctc tccgggcagg tgcttgtcag cggccatgat gttcgcgaat ataaactgga
      841 tcacctgcgt gatgcggtcg ctatggtatt acagaaaaat gttcttttct ccggaaccat
      901 tgcggataat ttacgctggg gaaatgaaaa cgctaccgat gaagaactgg ttagagtctg
      961 taaggtcgct caggcacatg attttattat gagctttccg gacggatatc aaaccgtgct
     1021 cggtcaggga ggagtgaatg tttccggcgg gcaaaagcaa cggctctgta ttgcccgcgc
     1081 attattgaaa aatccaaaaa ttattatttt ggatgacagt accagcgcga tcgatacggc
     1141 tacagaactg cgtttccgca aagccttcag agaaaattat cgggaaacaa cgattctgat
     1201 gatcgcccaa cgcgttcaat ccataaaaga ttcggatcga attttggtca tgaatgaagg
     1261 aaaaatcgat gccttcggaa cccatgatga attgattgaa accaacacga tttaccggga
     1321 agttttcgag tcccaacaga aaggagtcgc attatgaact ccgaaaaaaa gaatccgaac
     1381 cgctcaatta cacggactcc cggacccgga aaaaataaac caaggctcga aagaccgaag
     1441 gacattcggg gtaccctgat ccgaattctg aaaaccatgt cggattacaa attgcagttg
     1501 ggaatagtcg ccgttctgat ggcagtcagt tctctctcat cggttctcgg tacttatatg
     1561 ctgcgcccga tcataaacga ttatattatc ccattgatcg ctcaatcttc tccggatctg
     1621 tcgggtttaa tcggaatgct gttgaaaatg gcgggaatct attcagccgg cgttatcggc
     1681 aactatacgg ccagtcgact gatggtgaat atcagttccg gtaccctgcg gaaaattcgc
     1741 gtgggaatgt tcgaacatat tcaaaatctt cccatcaaat atttcgatac ccgaactcac
     1801 ggtgagatta tgagccattt caccaacgac gccgatactc tgcgcgaaat gatttcaaac
     1861 agcatcccga atttgatttc ctcggcgatt atgatcaccg gcgtgattgt tatgatgatt
     1921 attctgagct ggcaactttt tctgttggtt attttgcagc tgatcttaat tttcttcacg
     1981 attaaattta ttggcagccg aagctcaagt tattacatta aacaacaacg cgaattaggg
     2041 tcgctcaacg gttacattga agaaatgatc gaagggcaga aagttgtcaa agtatttacc
     2101 catgaaaaca aagtcaagaa gattttcaat caaaaaagcg aggacctttt taaggtggca
     2161 tccctcgcca atatgttttc tttggtgatt cagcctattt tcggaagtta ttcttacatt
     2221 cattatgcat taaccgccgc cgtcggaaca attatggcaa tccatgggat gctcgatatc
     2281 ggaacaatcg gcgccttttt acaatacacc cgaacgttta cccagccgat taaccagatg
     2341 tcacagcaaa tcaatgcgat tctggcggca cttgccggcg ccgagcgaat tttcatccta
     2401 atggatgagc cggtggaagc ggatgaaggc aacattgtcc tggttaacgt agagaaagat
     2461 cataacgagg tcattgtgga atctccggtt cataccggat attgggcttg gaaagatgtc
     2521 gatgaaaaag gtacggttac ctatactccg ctgaaagggg atatgcgttt cgaaaatgtc
     2581 tccttcgctt atgaaccggg aaatcc
//
LOCUS       DAOMIV010000307         2603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_43391_length_2603_cov_3.482732, whole genome shotgun sequence.
ACCESSION   DAOMIV010000307 DAOMIV010000000
VERSION     DAOMIV010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43391_length_2603_cov_3.482732"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="PKV59_07275"
     CDS             <1..203
                     /locus_tag="PKV59_07275"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HQP46589.1"
                     /translation="HSLLDDAKPVFETMPGWKCDITGARKYDELPVEARRYIEYVENE
                     IHCPMTYISVGPGRDEIILRKA"
     gene            200..1612
                     /gene="purB"
                     /locus_tag="PKV59_07280"
     CDS             200..1612
                     /gene="purB"
                     /locus_tag="PKV59_07280"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQP46590.1"
                     /translation="MKERYESPLAGRYASDYMIELFSEKNRHILWRKLWVELARSEHE
                     LGLPITSEQVKELEAHITDIDYAVVSAKEKEIRHDVMAHIYAYGEVCPKAKGIIHLGA
                     TSCYVTDNADLILYRQALSYIRNELWHVISILKDFALRYKAMPTLGYTHLQPAQLVTV
                     GKRACLWIQDLLNDAAELDFVLSQIRFLGCRGTTGTEASFMELFKGDSAKIDEMNRRI
                     AAAFGFQSLFPVCGQTYPRKLDSQILNVLSAVCQSCYKFANDLRLLQHLQQIEEPHEA
                     GQVGSSAMAYKRNPMRSERLCSLARFVMVDALNAPLTASVQWFERTLDDSANRRITLP
                     EGFLATDAVLRLMSNISRGLIVNEKIIERDVRQFLPFIATENLLMASVEKGGDRQISH
                     EIIRKHSLEVADRIKNGESGNLFELLANDPDFNLSAEEINSALNPQDFIGRCVEQVEN
                     FLNSFEIQYSSDFLEEEITL"
     gene            1655..2530
                     /locus_tag="PKV59_07285"
     CDS             1655..2530
                     /locus_tag="PKV59_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQP46591.1"
                     /translation="MKKKLTEKSSWIWSIVILGGIPLVIYILWRYLVSKNAFDKTLLI
                     HPYEIGTAKINRPVTLVLLTDLHNNFFGTEQNELVKAIQTVNPDVILMAGDMIDRKFP
                     LDGFLALIRNLPESVPAFFVTGNNDFWKGYPAGIKTILRDHGVNVLEGECVLINVKNQ
                     MIDVCGIDDPKIGKKIYHAQISSAGEQKNSGCYSILLMHRPDRRHLNEVLPYHYDLIL
                     SGHAHGGQWRTPGLINGLVAPDQGIFPRYTGGLYQHGETNQIVSRGLAKNSSLIPRIF
                     NRPELVVIQLLPDKR"
BASE COUNT          715 a          532 c          639 g          717 t
ORIGIN      
        1 ttcattcgct tctggatgat gctaaaccgg tttttgaaac gatgccgggt tggaaatgcg
       61 atattacagg tgcgagaaaa tacgatgaac tgcccgttga agcccgtcgt tatattgaat
      121 atgtcgaaaa tgaaattcat tgtccgatga cttatatctc cgtcggtccc ggcagagatg
      181 aaatcatttt gaggaaggca tgaaagaacg gtacgaaagc ccgcttgcgg gacgatatgc
      241 atcggattat atgatcgaat tattttccga aaagaaccgg catatccttt ggcgcaaatt
      301 atgggtggag ttggcgagat cggaacacga gctcggtctt ccgatcactt cagagcaggt
      361 caaagaattg gaagctcata tcaccgatat cgattatgcg gttgtctccg caaaagaaaa
      421 agagattcgt catgatgtga tggctcatat atatgcttac ggcgaagtct gccccaaagc
      481 aaaggggatt attcatctcg gagcgacaag ttgttacgtg accgataacg cggatttgat
      541 cctctatcgc caagcgttaa gttatatccg gaatgaatta tggcatgtca tctcgatttt
      601 aaaagatttc gcactgcgat ataaggcgat gccgactctc ggttataccc atttacaacc
      661 ggcgcaattg gtaacggtcg ggaagagagc ttgtctatgg attcaggatt tgctgaatga
      721 cgcagccgaa ctggattttg ttctcagtca aatccgtttt ctcggatgtc ggggaaccac
      781 gggcaccgaa gccagtttta tggagctgtt taaaggggac tcggcaaaaa tcgacgaaat
      841 gaaccggcgt attgcggctg cgtttggctt tcaaagtctt tttccggtat gcggacagac
      901 ttacccacgg aaattggatt cacagatttt gaatgttttg agcgccgtct gtcaaagttg
      961 ttataaattt gccaatgatt tacggctttt acagcattta cagcagattg aggaaccgca
     1021 tgaagccggg caagtcggtt cttctgctat ggcatataaa aggaacccta tgcgcagcga
     1081 gcgtttgtgt tcactcgccc gcttcgtaat ggtggatgca ttgaatgcac cgctgaccgc
     1141 gtcggttcaa tggttcgaaa ggacattgga tgattccgcc aatcggcgca ttactttgcc
     1201 cgaaggattt ttggcgaccg atgctgtttt acgtcttatg agtaatatca gtcgcgggct
     1261 gattgtcaat gaaaaaatta tcgaacgtga tgtgcggcaa tttcttccgt ttatcgccac
     1321 cgaaaatttg ttgatggctt cggttgaaaa aggcggcgac cggcagatct cgcatgaaat
     1381 tatccgaaaa cactcgttgg aagtggccga tcggattaaa aacggagaaa gcgggaatct
     1441 atttgagctg ctggcaaatg accccgattt taatttaagc gccgaagaaa taaattcggc
     1501 tttaaatcca caggatttta tcggacgatg cgtggaacaa gttgagaatt ttttgaactc
     1561 ctttgaaatt caatattctt cagacttttt agaagaggaa atcacccttt gatctttttt
     1621 tgataggggt taaaacaggg gggagcggtc ttcattgaaa aagaaactaa ccgaaaaatc
     1681 ctcatggatt tggtcaatcg taattttggg aggaattccg cttgtaattt atattctatg
     1741 gcgatatctt gtcagtaaaa atgcctttga taaaacactt ctgattcatc cctatgaaat
     1801 aggcaccgcg aaaatcaatc gacctgtcac tcttgtttta ctgacggatt tgcacaataa
     1861 ttttttcggg actgagcaga atgaattggt aaaagccatc caaacagtaa accccgatgt
     1921 gattctgatg gccggcgata tgatcgatag aaaatttccg ttagacggat ttttagcact
     1981 aattcggaat ttgcccgagt cggtaccggc tttttttgta acagggaaca atgatttttg
     2041 gaaaggttat cccgccggaa ttaaaacgat cttacgcgat catggggtta acgtactgga
     2101 aggggagtgc gtattgatca atgtgaagaa tcaaatgatc gacgtttgcg gaattgatga
     2161 tccgaagatc gggaaaaaga tttatcatgc ccaaatttct tccgccggag aacaaaaaaa
     2221 ttccggctgc tattcgattc ttctgatgca tcggcctgat cggcgccacc ttaacgaggt
     2281 tctgccttat cactatgacc ttattttatc gggacatgct catggcggtc agtggcgcac
     2341 accggggtta attaacgggt tggtcgcacc tgatcaggga atctttccca ggtatacggg
     2401 gggcctttat caacacggag agacgaacca aatcgtcagc cgcgggttgg caaaaaacag
     2461 cagcttaatt ccccgaattt tcaatcgacc cgaattggtt gtgattcagc ttctgccgga
     2521 taaacgctga atctcagtaa gggatctttc cggcccgccg gcgagaagaa ttgcagatcg
     2581 ccatcaaatc cgatactatc aag
//
LOCUS       DAOMIV010000308         2587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_43790_length_2587_cov_3.354660, whole genome shotgun sequence.
ACCESSION   DAOMIV010000308 DAOMIV010000000
VERSION     DAOMIV010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43790_length_2587_cov_3.354660"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="PKV59_07290"
     CDS             <1..472
                     /locus_tag="PKV59_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46592.1"
                     /translation="ILAERWGAGKIAVGIPFLLITVIGIIAIINLFSDEKLRQDQDNQ
                     KKNLFGAGLNQELLYGMLGETHETADILTNYPAYFLPGYGNCCEKVDFKDSVTFEQKL
                     SQSYIRYLLIPQFVENLSWESYDVLNRCTESGKCALLKCSNGYYIPYCLYTVNQ"
     gene            881..1348
                     /locus_tag="PKV59_07295"
     CDS             881..1348
                     /locus_tag="PKV59_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46593.1"
                     /translation="MKKIFIFFFALVLAFTAFYPASAQTETEETDVETQSDDDQLYYD
                     NLNQCFALLDLYKDDLDSFSSGKISTLTQKWKDTRNNLRWNSAVSCGYIMSIMEPEEL
                     KDYKSELVYGAYYQLVAIELYILSINNNNDPTLYSLYQQMAEAAQNNYSIIPK"
     gene            1491..1964
                     /locus_tag="PKV59_07300"
     CDS             1491..1964
                     /locus_tag="PKV59_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46594.1"
                     /translation="MKKMIIFSFVSVLMILCFTPVSAFSGSRQIDNETELSDDDYYAK
                     LEQCFSILDNYKEDLDSVYSGEIQTISENWHSIRDNLRWDVGVTCGYIMAAQEPEELS
                     DYAYHIFMGAYHEIAAVEFYIQCISNDNDPKLYDLYQEMLSIAEDFYSYIPLRGH"
     gene            2244..>2587
                     /locus_tag="PKV59_07305"
     CDS             2244..>2587
                     /locus_tag="PKV59_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46595.1"
                     /translation="MHYEISHPQLLEIRNGPYAETTFGPDQVWYPDRWLRKSGCGPTS
                     ASLLMAYYAFTRPELRSLYIADDLTRQSFIRQMQEMSRFIKPGMMGVDKISKYVDGVI
                     QFADEKGITLSP"
BASE COUNT          821 a          480 c          491 g          795 t
ORIGIN      
        1 gatccttgcg gagagatggg gcgccggaaa gattgcggtg gggattcctt tccttctcat
       61 caccgtaatc ggaatcatcg ccatcatcaa cctcttctcc gatgaaaaat tacgtcaaga
      121 ccaggataat caaaaaaaga atttgttcgg agccggatta aatcaggaat tattatatgg
      181 aatgctgggt gaaacgcatg aaactgccga tattctgacc aattatccag cctattttct
      241 ccccggatac ggaaattgct gcgaaaaagt cgattttaaa gattccgtca ccttcgaaca
      301 aaaactctca caaagttata tccgttattt gctcattcct caatttgtcg aaaatctttc
      361 ctgggaatct tatgatgttc tgaatcgttg tacggaaagc ggaaaatgtg cacttttaaa
      421 atgcagcaac ggctattaca tcccatattg cctatataca gtcaatcaat aatcttgtac
      481 aattagataa atatcaatac tgaattattt catgctatcg aaaaatccgc attattgagt
      541 gaatcgactc aaaaatcgga attgataaaa aaatttactc tttgtcgtta atcggattaa
      601 tcattaattc gggaacaaag ataaaaaata accaacttca caaatgatga atcttccgct
      661 gctaataaaa taaaaaaagg agggttatcg aatcagaaaa agttaaatac caatcatatc
      721 atttcggaag gagttttcct tccatatgga aacctttcaa agtttaaaag ttcctttgat
      781 cgactgacag atttcggatt aacattcaaa aatccgtttt tgtactcatc aaaagagcgg
      841 ttttgttatt tttataggta acaattagaa aggaaaagat atgaaaaaga ttttcatttt
      901 cttttttgcc ttggtacttg cctttacggc tttctatcct gcctcagctc aaaccgaaac
      961 ggaggaaact gacgtagaaa cccagtccga tgacgaccag ttatattatg ataatctgaa
     1021 tcaatgcttt gccttgttgg atttgtacaa agatgattta gattcctttt catccggaaa
     1081 aataagtacc ctcacccaaa aatggaaaga tacccgaaat aatctccgct ggaattcagc
     1141 ggtttcctgt gggtatatta tgtcaataat ggaaccggag gaattgaaag actacaaaag
     1201 cgaactcgtc tacggagctt attaccagtt ggttgcgatt gaattgtata ttttgagcat
     1261 taataacaat aatgacccaa cgctgtatag tttgtaccaa cagatggccg aagcagctca
     1321 aaataattac tcgatcattc caaaatagcc gtataaacgt caaatcactt ttaattatta
     1381 aatctttttt caaaacggtg agtcatcatt gaatcctgtg gaaatcatca aagaatacaa
     1441 ggaaaatcat ggctcaccgt ttctatattg aggaagagaa ggaataaatt atgaaaaaaa
     1501 tgattatttt tagttttgtt tccgttttga tgatcctgtg ttttacaccc gtaagtgctt
     1561 tttcgggttc ccggcagatc gacaatgaaa cggaattatc cgatgatgat tattacgcga
     1621 agcttgagca atgcttttct attcttgata attataaaga ggatttggac tcggtttatt
     1681 caggagaaat tcagaccatt tctgaaaact ggcatagcat ccgggacaat ctgcgctggg
     1741 atgtcggtgt gacctgcgga tatatcatgg cggcacagga gcccgaagaa ttaagcgatt
     1801 atgcctacca tatttttatg ggggcttatc atgaaattgc cgcggttgaa ttttatattc
     1861 agtgtatcag taatgataat gatccgaaat tatatgatct ttatcaagaa atgttatcca
     1921 tagctgaaga tttttactcc tatattccat taaggggaca ttaacatcgg aaagaaatcg
     1981 atattcttta tctgcggctt ttctcctcga gtaaattgat catgaaacgg atacagaaag
     2041 ggtgaaaata tctatcagcc tttttgtatc cgattttacg ttaatcctat ttctttagta
     2101 attctttttc ttatttccat acattaatat ttcgaatatt ttcgaaatcg agcgtgatct
     2161 gttgtataat atcgggttgc ttactaagaa aatgaaacag aaagcgcctg agttttcggg
     2221 aaaaaggtaa agatttagtc aatatgcact acgagatcag ccatccccaa cttcttgaaa
     2281 taagaaacgg accctacgcc gaaacaactt tcggtccgga tcaggtttgg tatcccgatc
     2341 gttggttacg aaaatctgga tgcggaccta catctgcatc tcttttgatg gcttattatg
     2401 cgttcacccg ccccgaactt cgtagccttt atattgcgga tgatctgacc cgacagagtt
     2461 ttatccgtca gatgcaagaa atgagtcgct ttattaagcc cggtatgatg ggggtggata
     2521 aaatcagtaa atatgttgac ggcgtaattc agttcgccga tgaaaaaggg atcacattat
     2581 ctcccca
//
LOCUS       DAOMIV010000309         2571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_44165_length_2571_cov_3.214626, whole genome shotgun sequence.
ACCESSION   DAOMIV010000309 DAOMIV010000000
VERSION     DAOMIV010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44165_length_2571_cov_3.214626"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PKV59_07310"
     CDS             complement(<1..1206)
                     /locus_tag="PKV59_07310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HQP46596.1"
                     /translation="MPSETQRVKVHLTALTYEGMALGHLPDGRVVFVAGGLPDEEVEI
                     EITGEKRDFVFARVIQILRPSVKRISPKCPHFGTCGGCHFQHLNYVDQLSVKRSVVEE
                     QLRRIGGIQNPVVGEVVPSPEIWQYRNTMQFHINSEGQICFRKAGGMETVPIHECYLP
                     AEPIRRLFPSLVFGPSDSGKRIEIRCDSDRNLMLIEEGHRIDLSDRLADGLSPTTQQT
                     DSSEKVESRKKVLFMDILGVRFQVSAGSFFQVNLSVAEAMIRYLQNNLPLDSKTNLFD
                     LYCGAGLFSRFFAEKVAHIDAIELSTDAASDFAANLAGRGAVSFRRGAVEQVLPQFRI
                     RGGAVILDPPRSGLKPRALDAIVRIAPSLIAYVSCDPSTLARDLRSLLAAGYRLERIT
                     PFDMFPQTYH"
     gene            complement(1273..1671)
                     /locus_tag="PKV59_07315"
     CDS             complement(1273..1671)
                     /locus_tag="PKV59_07315"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HQP46597.1"
                     /translation="MSESFFQAIRTSEIAPGGIRAIEINGRQLIICNADGKFYALSGR
                     CGHMNAPLERGTLDGIILTCPLHCAQFDIRTGEVLSGPVPMNFASDGEISSPQTRYLA
                     KVAELTERIHTESIATFRVKVEDGWILVAL"
     gene            complement(1664..2005)
                     /locus_tag="PKV59_07320"
     CDS             complement(1664..2005)
                     /locus_tag="PKV59_07320"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HQP46598.1"
                     /translation="MTIHVESRKQALRYFYTKIVEPMGYKFSPDNELVEFILEQEVNS
                     EGKYGIPFCPCQPIRHNRDHDMKIVCPCIPFHRKHFDAMRRCWCGLFVQQDVTDPDSL
                     PQLSAKEMGIE"
     gene            complement(2006..2257)
                     /locus_tag="PKV59_07325"
     CDS             complement(2006..2257)
                     /locus_tag="PKV59_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQP46599.1"
                     /translation="MEKVTMYTLSTCPWCRKTKKFFSDHNIPFEFTDYDLADEVTQSK
                     INDEMDAAGADGFPFVMIGDKIIVGYQPDVYARLLGLDK"
     gene            complement(2270..2527)
                     /locus_tag="PKV59_07330"
     CDS             complement(2270..2527)
                     /locus_tag="PKV59_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HQP46600.1"
                     /translation="MVSVYPLGVNTVIARVNGTVYAVAGKCLHMACPLFMGTLDSYIL
                     TCPCHDWRFDIRDGRFLDAPELSLKTYPTKTENGKIYIDFN"
BASE COUNT          697 a          627 c          586 g          661 t
ORIGIN      
        1 atggtaggtc tgaggaaaca tatcaaaagg tgttatccgt tcgagccgat atcccgctgc
       61 cagcaaggaa cgcaaatcac gggcaagggt tgacggatcg caagaaacat aggcgatcaa
      121 tgacggagcg attcgaacga tcgcatctaa ggctcgcggt ttaagcccgc ttcgaggcgg
      181 atccaaaatt accgctccgc cgcggattct gaattggggc aagacttgct caaccgctcc
      241 ccgccggaaa gaaacggctc ctctgccggc caaattggcg gcaaaatccg aagcggcatc
      301 ggttgagagc tcaatcgcat cgatgtgtgc taccttttcg gcaaaaaatc gagaaaataa
      361 accggcaccg caatatagat cgaataagtt tgttttcgaa tccagcggga ggttattctg
      421 taaatatcgg atcatggctt ccgcaaccga tagattgact tggaaaaaag agcctgcgct
      481 gacttgaaat cgaacaccta gaatgtccat gaaaaggacc ttctttctgc tttccacttt
      541 ttcagatgaa tctgtttgtt gtgttgtggg ggataagcca tccgccaacc ggtcagaaag
      601 atcgatccga tgtccctctt cgatgagcat caggtttcgg tcgctgtcgc atcggatttc
      661 aatccttttg ccggaatcgg aaggaccaaa aacgagggac ggaaagaggc gccgaatcgg
      721 ttccgccggc aaatagcatt catgaatagg caccgtttcc atgccacccg cttttcggaa
      781 gcaaatctgc ccttccgaat tgatatgaaa ctgcatcgta tttcgatact gccaaatttc
      841 cggcgaggga accacctcgc caaccaccgg attttggatt ccaccgatac gtcgtaactg
      901 ttcttccacg acggatcgtt ttacggaaag ttgatcgaca tagttcaaat gctgaaaatg
      961 gcaaccgccg caggtgccga aatgaggaca tttcggagag atgcgtttaa cggacggacg
     1021 caaaatttgt atcacacgag cgaagacaaa atctcttttt tccccggtaa tttcaatttc
     1081 aacttcttca tcaggcaacc cgcctgcgac aaaaaccacc cgcccatcgg gaagatgtcc
     1141 cagagccatt ccctcataag tcagggcggt caaatggact ttaactcttt gcgtttcgga
     1201 gggcatgtgt ttctcttcaa acaaaaatgt tatatattgt tgataaaatt tgatatttca
     1261 cgattccgga gttcacaaag cgaccaagat ccatccgtct tccaccttta ccctaaaggt
     1321 tgcgatcgat tcagtatgaa tccgttccgt cagttcagcc accttggcga gatagcgcgt
     1381 ctgcggagaa gatatttctc catcagaagc aaagttcatc gggaccggtc cggatagaac
     1441 ttctccggtt ctgatgtcaa actgcgcgca atgtaagggg caagtgagga ttattccgtc
     1501 caatgtcccc ctttcgagag gtgcgttcat atgtccgcag cgaccgctca aggcgtaaaa
     1561 cttaccgtca gcattacaaa taatcagttg acgaccgttg atttctatcg ctcggattcc
     1621 tccgggagct atctccgaag tgcggattgc ttgaaaaaaa ctttcactca attcccatct
     1681 cctttgctga taattgcggc aggctgtccg gatcggtgac atcttgttga acaaaaagcc
     1741 cgcaccaaca acgacgcatc gcatcgaaat gctttcggtg gaaaggaatg caggggcaga
     1801 cgattttcat gtcatggtca cgattatgac ggataggctg gcagggacag aaaggaatgc
     1861 catattttcc ttccgaattc acttcctgtt ccaggataaa ttcaaccaat tcattatccg
     1921 gagaaaattt atatcccatc ggttcaacga tttttgtgta gaaatatcgc aatgcctgtt
     1981 ttctgctctc aacatggatc gtcatttatt tatctaaccc taacaagcgc gcataaacat
     2041 ccggctgata accgacgatt attttatctc cgatcataac gaatgggaac ccgtccgcgc
     2101 cggcggcatc catttcatcg tttatcttcg actgcgtgac ttcatcggct aaatcgtaat
     2161 ccgtaaattc gaacgggata ttgtgatccg agaaaaactt cttcgttttt ctacaccacg
     2221 gacaggtgga taaggtatac atggtaactt tttccataaa agtttccttt caattgaaat
     2281 cgatatatat ttttccgttt tctgttttag tcggataagt tttgaggctg agttccggcg
     2341 catcaagaaa gcgaccgtcg cgaatatcga aacgccaatc atggcaaggg caggtaagaa
     2401 tatagctatc caaagtgccc ataaacaagg ggcaagccat atgcagacat tttccggcta
     2461 ccgcataaac tgttccgttt acccgggcaa taaccgtatt tacacccagc gggtagacgg
     2521 aaaccatgtt tccctcttgg atttatgttt cggaagagca ggaatttcaa g
//
LOCUS       DAOMIV010000310         2561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_44414_length_2561_cov_3.239425, whole genome shotgun sequence.
ACCESSION   DAOMIV010000310 DAOMIV010000000
VERSION     DAOMIV010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44414_length_2561_cov_3.239425"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /gene="mvaD"
                     /locus_tag="PKV59_07335"
     CDS             complement(<1..637)
                     /gene="mvaD"
                     /locus_tag="PKV59_07335"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HQP46601.1"
                     /translation="MSDSVATAVAHPNIAFIKYWGNRDDSLRLPANGSISMNLGALET
                     RTTVAFSSAYRSDRVSVNGSLSEGESLIRVVHFMDRIRSLAGEKRFAEIESRNNFPIG
                     AGIASSAAAFAALALAGSAAAGLHLNEAELSRLARLGSGSACRSIPPGFCEWLAGDSD
                     EESIAVSIAPPDYWALTDIIAVVSARHKEVGSSIGHQRAMTSPYQAVRVATA"
     gene            911..2026
                     /locus_tag="PKV59_07340"
     CDS             911..2026
                     /locus_tag="PKV59_07340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP46602.1"
                     /translation="MNILFVGNCTPYFSSGIVRAKRFFPSLSEAGISYDWMEINAAAV
                     QRWLERLDRSIFGKQKITDLIFRSLIHGLGLPYYWVQVLRILRKQSRYDVIFFQSVLL
                     PPRAMRYLAKQNSKIIFDYDDALYVKHESKTRGMIQYAWKVFAGSHALYDFAKAINPS
                     TILLPSSIPIEKYSVEATQEPHSPLRIGWIGGPSTLNQLEIVVDPLKNMLASGIDFEL
                     LIAGSKNQPIPLSKIEKLHILEIPEYSDDDIPEIVKKMDIGIMPLFDTPAEQGKCAMK
                     MLIYMAGELPVVASPVGESFYIVREGQNGFLAETAAEWEAKLSRLIGDADLRKKIGKA
                     GKQTVTERYSQDIIFRKMISEIFPDESIKNEPYANRT"
     gene            2077..>2561
                     /locus_tag="PKV59_07345"
     CDS             2077..>2561
                     /locus_tag="PKV59_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnan synthesis F family protein"
                     /protein_id="HQP46603.1"
                     /translation="MTFDESLSSERSQLSAFFDEGVRNRRMLSQAAFQLSDIASSKGW
                     KFICYCRQVKAIFSNSEVRFLPKIRNFLTATAKIVGLNHSDIFRPGPTSSSRVSPEEL
                     QIRLFGKKIEKNSEIAVVLHLFYVGLWPEIAELLSDIPGPFDLFISLPKEQKNFRSEI
                     Q"
BASE COUNT          721 a          593 c          589 g          658 t
ORIGIN      
        1 gagccgttgc cacccgaacc gcttgatacg ggctggtcat cgcccgttga tgccctatac
       61 tggaacccac ttccttatgc cgagcggaaa caaccgcgat aatgtcggtt aaagcccaat
      121 aatcgggcgg tgcaatggaa acggcgatcg attcttcatc ggaatcgccc gccagccatt
      181 cacagaaacc gggggggatc gaacgacagg ctgatccgga acccaaccgt gccaaacggg
      241 aaagttccgc ctcgttgaga tgcagcccgg cggccgcact tccggctaat gctaaagcgg
      301 caaaagcggc agctgaggaa gcaatgccgg ctccaatcgg aaaattattt cgactctcga
      361 tttccgcaaa gcgtttttcc ccagccaggc tccgaatgcg atccataaaa tggacaaccc
      421 gaatcaacga ttctccttcc gataaggagc cgttcacgga aacccggtcg gaacggtatg
      481 cggaagagaa agcgacggtg gtccgcgttt ccaaggcccc gagattcatg gaaatcgatc
      541 cgtttgccgg taaacgcaag gaatcatccc gattgcccca atatttaata aaagcgatat
      601 tcggatgtgc gacagcggtc gcaacagaat cagacatata ccctcataga tggttgataa
      661 tttgctacgg taagtttatc atggaccgca accgttgcaa taagaagttt ctcttctgac
      721 tcttttctat aaaattttca atcttgatca ggaaaccggg gcttttagag aaattattgg
      781 ttgttctcga ttgattttca aacatcccgg tttgcttctg tattcatgcg acagacgatg
      841 cattgatttg aataaaaatc tcacgttata atcaaattca aatctgattg atcaaccgcg
      901 gggaaatctt ttgaacatcc ttttcgtcgg gaactgtaca ccttattttt caagcggcat
      961 tgtccgggca aaacggtttt ttccgtctct ttcggaggcg ggtatttcct atgattggat
     1021 ggagattaat gccgctgccg tacaacgttg gctcgaacga ttggatcgat cgatcttcgg
     1081 aaaacaaaaa ataaccgatt tgatctttcg atcgctgatc cacgggctcg gattaccata
     1141 ttattgggtt caggtcttgc ggatattgag aaaacaatcc cgttatgatg tgattttctt
     1201 tcagtctgtg ctcttacctc cccgggcgat gcgatacttg gcgaaacaaa attcaaaaat
     1261 cattttcgat tatgatgacg ctctctatgt gaagcatgaa tcaaagaccc gtggaatgat
     1321 tcaatatgcg tggaaagtat ttgccgggag ccatgcactt tatgactttg cgaaagcgat
     1381 caatccttcg acgattttat tgcccagcag tataccgatt gaaaaatatt ccgtcgaagc
     1441 cacacaggaa ccccattctc cgctgcggat cggttggatc ggaggacctt caaccctgaa
     1501 tcagttggaa attgtagtcg acccgttaaa gaatatgctt gcttccggca tcgattttga
     1561 gctgttgatc gccggatcaa aaaatcagcc gattcctctg agcaaaatcg aaaaattgca
     1621 tatacttgaa attccggaat attcggatga cgatattccg gaaattgtca aaaaaatgga
     1681 tatcggcatc atgccgctat ttgacacccc ggcggaacag ggaaaatgtg ccatgaaaat
     1741 gttgatttat atggccggag aactaccggt ggttgccagt ccggtcggtg agtcgttcta
     1801 tattgttcgg gaaggtcaaa acggttttct tgccgaaaca gcagccgaat gggaagccaa
     1861 actcagtcgt ttgatagggg acgcagacct cagaaaaaaa atcggcaaag ccggcaaaca
     1921 gaccgttacg gagaggtatt cacaggatat tatttttcgg aaaatgattt cggagatttt
     1981 tccggatgag tcgataaaaa atgaaccata cgcgaatcgc acataaacaa acgacggaat
     2041 ggcaggatca agaacaaata ggtcggaggc aaaaaaatga cattcgatga atccctcagc
     2101 tcggaacgca gtcaactaag cgcctttttc gatgaaggag taagaaaccg acgcatgctg
     2161 tcacaagctg cctttcagct ttccgacatc gcatcaagca aaggttggaa attcatttgc
     2221 tattgtcgcc aggtcaaagc aattttctca aattccgaag ttcgttttct cccgaaaata
     2281 cgcaattttt tgactgccac tgccaagatt gtcggtttga atcattcgga tatattccgc
     2341 cccggtccga caagttcttc ccgagtgtca ccggaagagc tgcaaataag gttattcggt
     2401 aagaaaattg aaaaaaattc cgagatcgct gtcgtcctcc acctttttta tgtcggcctt
     2461 tggccggaaa tagcggaact gctatctgat attcccggac ccttcgatct ttttatctct
     2521 ttaccgaaag aacaaaagaa tttccgttcg gaaattcaaa a
//
LOCUS       DAOMIV010000311         2553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_44641_length_2553_cov_3.658927, whole genome shotgun sequence.
ACCESSION   DAOMIV010000311 DAOMIV010000000
VERSION     DAOMIV010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44641_length_2553_cov_3.658927"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..2460
                     /locus_tag="PKV59_07350"
     CDS             64..2460
                     /locus_tag="PKV59_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQP46604.1"
                     /translation="MKLYEDFILRDHYDVIVIGAGLGGMAAAGLLAKRGLSVLMIEQQ
                     NKPGGACTSFKREDHIHDVGAAMLYGFGQKGFRPFHFLLNELEEPIDMISHSTLARMN
                     FEGKEIIFWPDLQRFIDELIELFPDEKEGIRAFYADLYKMYENIVIKNEVIVPPSEYS
                     PRQGLRRLINDPFSIFKMQKLLSTSTMDLLKKYFHSPELFNFYDKLCSAYAYTTAEET
                     PAVLAATMFIDNHTGGVYYPAGGAQMLPNTIEKAFERYGGQALYHQLVDEILIEDGRA
                     YGVRLQDKTEIFADRVIANATVWNIYGHLVRQQHISPDRLEWAQSLIPTFPSMTLYMV
                     VDREAIPEDSFPWEIFIENRKIIDSSDLTLYINSLVDHSLCPPDELVVMAISPNFCSW
                     PHMDDPTYRSPEYEDQKRREAERMIDQIEQHIPGFKQHIRSLIIGSPTTIERYLLKNG
                     GAVGGPKNQIGQEMLKRLHARSEWKNLYFCGDSTVMGTGAPATVVSGIGAANVILREL
                     HQPEYDTHVFPEEVVHFVQPLYHRPDYISGTPVSVENAFLAAAKCQGCLHPRCRKGCP
                     AGIDIPGFLRRMEAKNYIGAARLIHEKNPFGETCGLTCAANHLCQQFCYRRDFSGEPV
                     NIAELERWVCEQAGEDGWQKHEKSTVNGEIAIIGGGLAAMSCAYYLALSGWGISIFAP
                     ENQPGGKLSEKMRSDPVLQASFNRDIRRIFAEEIRFFGNQNPVHEADLKRMLKEYACI
                     FITKDQFSERENFDEVKKKSQGLFLGDEFLINGISPVEAIACGRKKAVEIYQYLRNDA
                     "
BASE COUNT          735 a          560 c          574 g          684 t
ORIGIN      
        1 aaccggaaaa ttagctgctg aagcggtatt acatgactca aataaaaagg ccgaacacaa
       61 aaaatgaaac tttatgaaga ttttatttta agggatcact atgatgtaat cgttatcggc
      121 gcaggtctgg gtggaatggc agcagccgga ttattggcaa aacgaggtct ttccgtttta
      181 atgatcgagc aacaaaacaa gcccggcgga gcctgtactt catttaaacg agaagaccat
      241 attcatgatg taggagccgc catgttatat ggtttcggtc aaaaagggtt ccggccgttc
      301 cattttttac tcaatgaact cgaagaaccg attgatatga tttcccattc cactttggcc
      361 cgaatgaact ttgaaggaaa agaaattatc ttttggccgg atctgcaacg atttatcgat
      421 gaattaatcg agttgtttcc ggacgaaaaa gaaggcatta gagcttttta tgcggatctc
      481 tacaaaatgt atgaaaatat cgtcattaag aatgaagtca tcgtccctcc ttccgaatat
      541 tccccgcgcc agggtttacg gcgtctgatc aacgatccgt tttcgatatt taaaatgcaa
      601 aagttactat ctacttccac catggatctt ttgaaaaagt atttccattc tcccgagtta
      661 ttcaattttt acgataaact ttgctccgct tatgcatata ccaccgcaga agagacgccg
      721 gcggttctgg ctgcaacaat gtttatcgac aatcataccg gcggagttta ttatccggcc
      781 ggcggtgcac aaatgctccc gaatacaatc gaaaaagctt tcgagcgtta tggcggtcaa
      841 gccctttatc atcaactcgt cgacgagatc cttatcgaag acgggcgcgc ttatggtgta
      901 cgactgcaag ataaaaccga aatatttgcc gatcgggtga tcgccaacgc aaccgtctgg
      961 aatatttacg gacatctggt acggcaacag catatttctc cggatcgtct tgaatgggcg
     1021 caatctttaa ttcccacttt tccgagcatg acactttaca tggttgtcga tcgggaggct
     1081 attccggaag attcttttcc ttgggaaatc ttcatcgaaa atcggaaaat aattgattca
     1141 tctgatttga cgttatacat aaactcactc gttgatcact cactttgtcc cccggatgaa
     1201 ctggttgtaa tggcaatctc accgaatttt tgctcttggc cgcatatgga tgatccaact
     1261 taccgttcac cagaatatga agatcaaaaa cgtagagaag cggagcgaat gattgatcaa
     1321 attgagcaac atattcccgg ttttaaacaa cacatccgct cattaatcat cggatcaccg
     1381 accacgatcg aacgatactt acttaaaaac ggtggagcgg tcggcggtcc caaaaatcaa
     1441 atcggtcagg aaatgctcaa acgactgcac gcacgcagtg aatggaaaaa cctctatttt
     1501 tgcggagact caaccgtgat gggcaccggt gctccggcaa ccgtcgtatc cggaatcggc
     1561 gcagcgaatg ttattttgcg cgaattgcac caacccgaat atgataccca cgtcttcccc
     1621 gaagaagttg ttcattttgt tcaacctcta tatcacagac cggactacat ttccggcaca
     1681 cctgtttcgg tcgaaaatgc atttttggct gcagcgaaat gtcagggatg tcttcatccg
     1741 cgatgcagaa aaggttgccc tgcgggaatt gatattccgg gatttttgcg caggatggaa
     1801 gcaaaaaatt acatcggggc agcccgcctg attcatgaaa aaaatccttt cggcgaaacc
     1861 tgtggcttaa cctgcgcggc gaatcatctt tgccaacaat tttgttatcg ccgcgacttc
     1921 agcggggaac cggtaaatat cgccgaactt gagcgatggg tttgcgagca agcgggagag
     1981 gatggttggc aaaaacacga aaaatcgacc gtgaacgggg aaatcgccat catcggcgga
     2041 ggattggctg caatgagctg tgcttattat ctggctctca gcggatgggg aatcagtatt
     2101 tttgcgccgg aaaatcaacc gggaggcaaa ttatcggaaa aaatgagatc cgatccggtt
     2161 cttcaagcat ctttcaatcg cgatatccgg agaatttttg ccgaagaaat acgctttttc
     2221 ggaaatcaga atccggttca tgaggccgat ttgaaaagaa tgctcaaaga atatgcgtgc
     2281 atttttatca caaaagacca attttcagaa cgagaaaatt ttgatgaggt caagaaaaaa
     2341 tcccagggac ttttcttagg cgatgagttt ttaataaatg gcatctcacc ggttgaagct
     2401 atagcttgcg ggcgcaagaa agccgttgaa atttaccaat atttacggaa tgatgcatga
     2461 ctcaatgtat tttttcataa tcaaatttgg aatagacaaa gctctggtat tcacgcaccg
     2521 tattgcgcac gctttttata cttgttcgtt ccg
//
LOCUS       DAOMIV010000312         2552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_44665_length_2552_cov_4.130156, whole genome shotgun sequence.
ACCESSION   DAOMIV010000312 DAOMIV010000000
VERSION     DAOMIV010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44665_length_2552_cov_4.130156"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="PKV59_07355"
     CDS             <1..1339
                     /locus_tag="PKV59_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP46605.1"
                     /translation="SWFGDRIRKALSPWLKWEPQPSDVRWIIFFLWALLSVNNMFNLN
                     YQVGTDGWGHIEYIDYIAIKGTLPLAPEGWHMFQPPLNYLLSAPLYAVLIKCLDLPSV
                     VKIMTIVPILCGLMQIEIVYRAAKLVFTDRKDLQIIAIITGSFLPIHTYACQYVGNEP
                     LTACLVSLVILLCLFLIVPEQEQRGYGYFILIGLIWGLALLSKITAMLLAPVLIMVII
                     FHAGSVKKPLRSAWRPVITVFFVAMIVAGWYYLRNYIKLGNAFMGLFDPLTMLQWWQD
                     PGYRMWPHLWSFGEALHKPVYAGLTSFWDMFYATLWLDGINSGLVDFIPWNRNFMIAG
                     TLLALVPSVFIVTGVIFQALNNDAKQKRAVILSVILILLFLAAMIDMYIVCPLYSRTK
                     ASYTLGLLPCYAILIAAGAEPFLRNRIIRAIALGLFACWAFAAYAAYFVPGFQ"
     gene            complement(1446..2450)
                     /locus_tag="PKV59_07360"
     CDS             complement(1446..2450)
                     /locus_tag="PKV59_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46606.1"
                     /translation="MMLAASLINISYEDVYVAPESGSNGQEYYVLPDNSVVRRDHTAT
                     VISMGEGMSKMLLIDGIGTTSLRILTKMMAHLPLAFCREKPQSALVICLGMGTTYRSA
                     LSWDIQTTAVELVPSVKEAFGYYFADAEKILNNPDGKIIIDDGRRFLKRTTETFDVIT
                     IDPPPPLESAGSSLLHSEEFYGLVKKHLKKNGIFQQWFPCGELKIAQAIARSLSNTFP
                     YIRIYQSVEGWGLHFLASMNPIDIPAPRVMLSRIPEKARKDIMEWYQVDQTTNLEGFI
                     KFIFKSEIPFAELLNDDQEIIITDNKPFNEYFFLRRTLDNIKGQYREVSCLPSQNDKY
                     "
BASE COUNT          634 a          553 c          588 g          777 t
ORIGIN      
        1 ttcctggttt ggcgaccgga tccgaaaggc gctgtcacca tggttgaaat gggaacctca
       61 gccttccgat gtccgctgga tcatcttttt cttatgggcg ttgttgtcgg tcaacaacat
      121 gttcaatctt aattatcagg ttgggaccga tggctgggga cacattgaat atattgatta
      181 tatcgcaatc aaaggaacgc ttcctctcgc tcccgaaggc tggcacatgt ttcaaccgcc
      241 gctaaattac ctgctaagcg ctcctcttta tgcggtgctg ataaaatgtc tggatcttcc
      301 gtccgtcgta aaaatcatga cgattgttcc catcctgtgc ggactcatgc agattgaaat
      361 tgtgtaccgt gcggcaaagt tggtttttac ggacagaaaa gacttacaga tcattgccat
      421 catcacaggt tccttccttc ccatacacac ctatgcctgt cagtatgtgg gtaacgaacc
      481 gcttaccgca tgcctcgtat cgttggtgat tcttttgtgc ttgttcctga tcgtgcctga
      541 gcaggaacaa cgaggatatg gatattttat attgatagga ttgatatggg ggctggctct
      601 tctttccaaa atcactgcca tgttactggc tcctgtgttg atcatggtga taatttttca
      661 cgcagggtcg gtgaaaaaac cgttgaggtc tgcatggcgt ccggtgatta cagtattttt
      721 tgttgcgatg atagttgccg gctggtatta tttaagaaat tacatcaaat taggcaatgc
      781 ttttatggga ttgtttgatc ccttgacaat gctgcaatgg tggcaggatc ccggttatcg
      841 catgtggccg catttatggt ctttcggtga ggcactgcac aagcccgttt atgccggttt
      901 gacaagcttc tgggacatgt tttatgcgac gttatggctt gatggtatca attcgggact
      961 ggtggatttc attccctgga accggaattt catgattgcc ggaacactcc tggctttggt
     1021 gcccagtgtt tttatcgtaa cgggtgtgat ctttcaggca ttgaataatg acgcgaaaca
     1081 aaaacgtgct gttattcttt ctgtcatctt gatcttactt tttctggcag ccatgatcga
     1141 tatgtatatt gtttgtcctc tgtatagcag aacgaaagcc agttatactt taggactttt
     1201 accctgttac gcgattctta ttgccgcggg agcagaacca ttcctgcgaa acaggattat
     1261 tcgtgccatt gctctgggtt tgtttgcctg ctgggcgttt gccgcctatg cggcgtattt
     1321 tgttcctggc ttccaataaa aagtcggcat gtccatcacc gtctatttta aataaatagc
     1381 gcaccgtcat cgcggagtga aatagcatag agtgtccggt aattcccggg tgccatgcac
     1441 aaaatttagt atttgtcatt ttgggaaggc agacaggaaa cctcccgata ttgtcctttg
     1501 atattatcca gggttctgcg cagaaaaaaa tattcattaa aaggtttgtt gtcagtgatg
     1561 atgatttctt gatcgtcgtt taataattct gcaaagggta tctcactctt gaaaatgaat
     1621 tttataaagc cttccagatt tgtggtttga tcaacctgat accactccat aatatctttt
     1681 ctggcttttt caggtattct tgacagcatg acccggggcg ctggaatgtc aatcggattc
     1741 atcgaggcca gaaaatgcaa cccccagccc tcaacggatt gatagatccg tatatagggg
     1801 aacgtattgg ataaagatct cgcgatcgcc tgggcaattt tcagttctcc acaggggaac
     1861 cattgttgaa aaattccatt tttcttgaga tgttttttaa ccagaccata aaattcttcc
     1921 gaatggagaa ggcttgatcc ggctgattcc agcgggggcg gcggatcaat cgttatcaca
     1981 tcaaaagtct ctgtcgtcct ttttaaaaaa cgccgtccgt catcaataat aatcttaccg
     2041 tcaggattat taagaatttt ttcggcatct gcgaaataat acccgaaggc ttctttaacg
     2101 ctggggacaa gttcaacggc agttgtttgg atatcccaac tcaaggctga acgatacgtg
     2161 gtgcccattc ccaggcaaat gaccagcgcg gattgaggtt tttcccgaca aaaagccagc
     2221 ggcaaatgag ccatcatttt tgtcaagatt ctcaggctgg ttgttccgat tccgtcgatt
     2281 aaaagcattt tgctcattcc ttcccccatg gaaatgacgg tggcggtatg gtcccgtcga
     2341 acaacgctgt tatccggcag gacatagtat tcttggccgt tcgagccact ttccggtgct
     2401 acatacacgt cttcatagct gatattgatt aacgaggctg cgagcatcat gcacgtgatc
     2461 ataatgacta ctgcagaaaa tcgtatcgta gagaaaaata tttttaggga gcatgacata
     2521 aaaaataaca gaaaaggcac actcaaaaaa at
//
LOCUS       DAOMIV010000313         2537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_45084_length_2537_cov_2.895649, whole genome shotgun sequence.
ACCESSION   DAOMIV010000313 DAOMIV010000000
VERSION     DAOMIV010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45084_length_2537_cov_2.895649"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PKV59_07365"
     CDS             <1..735
                     /locus_tag="PKV59_07365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HQP46607.1"
                     /translation="IPELDWIAETDQRIVGGIISSKAQVVDYNGLNHPIICLGPIGVL
                     PAYQGLGIGKKLISKSLEEAERTGFQAVVVLGDPAYYAQFGFEKASNYHIRASEAENP
                     DNLLVKELRPKGLEGVTGKVLFDPAFQVDEKAVNEFDRDFPAKQKKEAEPEVKESNLD
                     DDQKWKRYNVIIGISLGIILGFILFGIPQKYLDDGLRLTIMLVIILFFPQIFEKKFQV
                     KFRLGRLWMAGTLIFCTILFIFLKLI"
     gene            complement(793..1347)
                     /locus_tag="PKV59_07370"
     CDS             complement(793..1347)
                     /locus_tag="PKV59_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQP46608.1"
                     /translation="MFNQSDLPEWQRDATSPDQYHCCETRRGREFIVRMMTGSDPVLA
                     IQKFAKEQNIRFGKVHATFMGGFQPCHYYVWVPDIAHPDNWHLEGEAINTNLTMLCAI
                     GGMIGQRPTDDGGEEPFVAMHFVAGGAWDTGTIAGHLLEGTRVKGCMQVFVTELLDIE
                     VLKPVDVFGEAYTHPENFYKNVTK"
     gene            1607..2155
                     /locus_tag="PKV59_07375"
     CDS             1607..2155
                     /locus_tag="PKV59_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46609.1"
                     /translation="MNLVNLLLDSFSVQPNSLGRADIYKDYGFGEKMRLSYQLDQISY
                     EIGMINSFIEMVACGVKRLAISPPIDPSNLNIMLEASEIISEGFGTKYRFEESLIVTD
                     IQSAEFTEGKNSILYYATDDAIAEYLDLKEKIKSLTEKNQYFGQKRREISFAFGRLLG
                     YPDEVILRKIDSPSHIDPFVLI"
     gene            2289..>2537
                     /locus_tag="PKV59_07380"
     CDS             2289..>2537
                     /locus_tag="PKV59_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQP46610.1"
                     /translation="MKLYLKTPLFPLTRINQKFHKNFQIKMECYQPSGSFKIRGISYV
                     CLDAKKKGISHLICASGGNAGYAVAYCARTLGMKCTIVI"
BASE COUNT          819 a          442 c          518 g          758 t
ORIGIN      
        1 atacccgaac tggattggat cgcggaaacg gatcagcgga ttgtcggcgg cattatctcc
       61 agtaaggcgc aagttgttga ttataacggg ttaaatcatc cgataatttg tctgggtccg
      121 atcggagtct taccggcata tcaaggcttg gggatcggga aaaagttaat ctctaaatca
      181 ttggaagagg ctgaaagaac aggatttcaa gcagttgttg tgttgggtga tccggcttat
      241 tacgcgcaat ttggttttga aaaagcaagc aattatcata ttcgcgcctc cgaagcggag
      301 aaccccgata atttattggt gaaagaatta agaccgaaag gattggaggg cgtcaccggg
      361 aaagtattat ttgatccggc ttttcaggta gacgagaaag cagttaacga atttgatcgc
      421 gatttccctg ctaaacaaaa aaaagaggcg gaaccggaag ttaaagaatc gaacctggat
      481 gatgatcaaa aatggaaacg atacaacgtg atcatcggta tttccctggg gatcatttta
      541 ggatttatct tatttggaat tccgcaaaaa tacttggatg acggtttgcg attaaccatt
      601 atgctggtca tcattctgtt ctttccgcaa atatttgaga agaaatttca agttaaattt
      661 cgtctcggac ggctttggat ggcgggaacg ctgatatttt gtacaattct atttatcttt
      721 ctgaagttaa tatgacaaag aaggaggtcg tctactgata gacctccttc tttataaaca
      781 aaaatttaat gattattttg ttacgttttt ataaaagttt tccggatgag tataagcttc
      841 tccgaaaaca tccaccggtt tcagaacctc aatatccaat aattcggtaa caaaaacctg
      901 catacagcct ttaactctgg ttccttcgag caaatggcct gcaattgttc cggtatccca
      961 tgctcctcct gcgacaaaat gcatcgcgac aaaaggttct tctccgccat catcggtagg
     1021 gcgttgtccg atcattcctc ctattgcgca taacatagtc aaattcgtat tgattgcttc
     1081 accctcaaga tgccaattat ccggatgagc aatatccgga acccaaacat aataatgaca
     1141 gggttgaaat ccgcccataa atgtggcatg gactttaccg aaacggatat tttgttcttt
     1201 tgcaaatttt tgaatcgcaa gaacgggatc ggaaccggtc atcatacgaa caataaattc
     1261 tcgcccgcga cgagtctcac aacaatgata ttggtcagga gaagtagcat ctcgctgcca
     1321 ttccggaaga tcagattgat taaacatatt ctttccttta taagaagatg atggaaaaac
     1381 aacgcgtatt atactacaag ctttcatttc acaagaagaa aataaaaaca tttttcctaa
     1441 atttctggat atatgataaa aatggaaata tagttatatt taataataaa gagaaattaa
     1501 gcaagccgat ttacggaggg aatagtgatt tagaatattt attttggttg ctatatgttt
     1561 ctatcgatat tttatagcta tctcgggata aaatcgatat tcctagatga atcttgtaaa
     1621 cttattgttg gattcgtttt ctgttcagcc taattctctc gggagggctg atatttataa
     1681 agattatggc tttggagaaa aaatgcgatt atcttatcaa ttagatcaaa tttcctatga
     1741 aatcggaatg ataaactctt ttattgagat ggtagcttgc ggggtgaaaa ggcttgcgat
     1801 cagccctcct atcgatccat caaatctcaa tattatgtta gaggcttccg aaattatcag
     1861 cgaaggattc ggtacaaagt accgttttga ggaatcatta atcgttaccg acattcaaag
     1921 cgcagaattt acagaaggaa agaattctat tttgtattat gcgacggatg atgcaatcgc
     1981 tgagtacttg gatttaaaag aaaaaattaa atcactcaca gaaaaaaatc aatatttcgg
     2041 acaaaaacgg cgagagatat cctttgcgtt tggccgatta ctggggtacc ccgatgaggt
     2101 tattctaaga aaaattgaca gtccaagcca tatcgatccg tttgttttga tataaataac
     2161 gaatattttt tataattcgg aaacgacaaa ataagacgga ctggattatg aagaatgaat
     2221 ttatacgcca aaaagaacga tatactcaat gtaaattacg ataaaaaaat tgaaatcagg
     2281 cataagatat gaaattatat ctaaaaactc cgttatttcc tttgactcgt attaatcaga
     2341 aatttcataa gaattttcaa attaaaatgg aatgttatca accgagcggt tcttttaaaa
     2401 ttcgtggaat ctcatatgtt tgcttggatg ccaagaaaaa gggaataagc catcttatct
     2461 gtgcttcggg aggaaacgcg ggttatgcgg tcgcttattg cgccagaact ttgggaatga
     2521 aatgtacgat cgtaatt
//
LOCUS       DAOMIV010000314         2531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_45244_length_2531_cov_3.898223, whole genome shotgun sequence.
ACCESSION   DAOMIV010000314 DAOMIV010000000
VERSION     DAOMIV010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45244_length_2531_cov_3.898223"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="PKV59_07385"
     CDS             <1..814
                     /locus_tag="PKV59_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46611.1"
                     /translation="HAKECAALFKQHMDEIDGILVSLPNFGDEKGIADTIKLSGLRKP
                     ILVQAFPDDLDKFDVANRRDSFCGKISVCNNLRQYGYDFSLTTDHCVYPYEESFKADL
                     KKFAGVCRVVNGLKHARLGAIGARPNAFNTVRYSEKLLEKAGISVLTADLSEIIGPVN
                     RLSDSDLAVKKALEAIDSYIPHEGIPSEALIKMAKFKTVLDRWIQDNEITGTAIQCWN
                     SVQENLGINVCTLMSMMSELDSFRLRNGYHRSAFDVYTAISRKCTGNFGGLE"
     gene            759..1238
                     /locus_tag="PKV59_07390"
     CDS             759..1238
                     /locus_tag="PKV59_07390"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46612.1"
                     /translation="MYTLQLAGNAPAILVDWNNNYASKKDECVLFHCGNFAKSYVPNA
                     CMGNAKILATTMGTENTMGTIEGRVVGDKMTYARVSTDDVNGMIRAYTGEGKFVDDKL
                     DTFGARAVVEIPDLQELMHFICLNGFEHHVAIAKENLSDIIVEAFEGYMGWATYLHS"
     gene            1309..1707
                     /gene="rbsD"
                     /locus_tag="PKV59_07395"
     CDS             1309..1707
                     /gene="rbsD"
                     /locus_tag="PKV59_07395"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HQP46613.1"
                     /translation="MKKNGILHGEISKTIAELEHYDTILIGDAGMPCPDGVKWIDLAV
                     SEGVPSFFDVLTAVLSELEVQRAVISREMKDRSPEAYARLRDILENDCPIEEIPHEEL
                     KQTSKKCKAHIRTGEFTPYCNVILEGGVTF"
     gene            1749..>2531
                     /gene="rbsK"
                     /locus_tag="PKV59_07400"
     CDS             1749..>2531
                     /gene="rbsK"
                     /locus_tag="PKV59_07400"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HQP46614.1"
                     /translation="MNQPKICVAGSSNMDLVTSVKRMPLPGETIHGIDFHTFFGGKGA
                     NQAIMAAKLGGRVTMIAKVGNDGFGQQYLENYQSSGLVTDYVSVTDEAPTGIAAITVD
                     EQGQNSIIVVSGANFAMTPSDIENATPAVREADAALCQLEIPLDATIRFLEIAKDANV
                     LTIFNSAPAQELPEKIYALSDYFCPNETEAALMAGFPVKTIEDAEKAGRLFIGRGAKS
                     VIVTMGGLGSLFVSADKVFHIPAEKVKAVDTTGAGDAYIGSIA"
BASE COUNT          689 a          490 c          683 g          669 t
ORIGIN      
        1 ccatgccaaa gaatgtgccg ctttatttaa acaacatatg gatgagatcg atggaatttt
       61 agtcagcttg cccaatttcg gcgatgaaaa gggaattgct gacacgatca aattatccgg
      121 cttacggaag ccaattttgg tgcaggcttt cccggacgat ttggataaat tcgatgtggc
      181 gaaccgcaga gattcatttt gcggaaagat ttcggtttgt aataatttac gccaatatgg
      241 atacgatttt tctctaacta ccgatcattg cgtttatcca tatgaagaaa gtttcaaagc
      301 ggatctgaag aaatttgccg gtgtttgccg tgtggtgaac ggcttgaaac atgcccgctt
      361 gggggcgatc ggggctcggc cgaatgcttt taataccgtt cgttatagcg aaaaattact
      421 ggagaaggct ggtatttctg ttttgacggc ggatttatcg gaaattatcg gtccggtaaa
      481 ccggctctct gatagcgatc tagcagttaa aaaagcactt gaagcgattg attcttatat
      541 cccccatgaa ggtatcccga gtgaagcatt gatcaaaatg gcaaaattca agactgtttt
      601 ggatagatgg attcaggata atgaaataac agggacagcc attcagtgct ggaattccgt
      661 tcaggagaat ctgggtatca atgtttgtac tctgatgagc atgatgagcg aacttgattc
      721 cttccgcttg cgaaacggat atcaccggtc tgctttcgat gtatacactg caattagccg
      781 gaaatgcacc ggcaattttg gtggactgga ataataatta tgccagcaag aaggatgaat
      841 gcgtgttgtt ccactgcgga aactttgcaa agagttacgt gccgaatgcc tgtatgggaa
      901 atgcgaaaat tctggctaca acgatgggga ccgaaaatac gatggggacg atcgaaggac
      961 gggttgtcgg agataaaatg acttatgccc gggtttcaac agacgatgtt aacggtatga
     1021 ttcgtgctta tactggagaa gggaaatttg tggacgataa actggacact ttcggcgcta
     1081 gagcagttgt tgaaattccg gatttgcagg agttgatgca ttttatctgc ctgaatggat
     1141 ttgagcatca tgtggctata gcaaaggaaa atctgtctga tattatagtc gaagctttcg
     1201 aaggatatat gggctgggcg acttaccttc acagttgaat ttcgatcgat gatcgatagt
     1261 gattttcgtt cccttctgtt ggtgaacgga acgggggagg ttaaataaat gaagaaaaac
     1321 ggtattctcc acggagaaat ttcgaaaacc attgctgaat tggagcacta tgataccatt
     1381 ctcatcgggg atgcgggaat gccttgcccg gatggcgtca agtggatcga cctggcggtt
     1441 agcgaagggg tcccgtcgtt ttttgacgta ctgacggctg ttttaagcga actcgaagtt
     1501 caaagggctg ttatcagccg tgagatgaaa gatcgcagtc cggaggcgta tgctcgcctg
     1561 cgggatattt tggaaaatga ttgtccgatc gaagagattc cgcatgagga attgaaacag
     1621 acatccaaaa agtgtaaagc ccatattcgt accggcgaat ttacaccgta ttgcaatgta
     1681 atccttgagg ggggcgtcac tttctgaacc gcccctccaa attattgttc gcaagagaag
     1741 gaacctctat gaatcagcct aaaatctgtg ttgccggatc ttccaatatg gatctggtta
     1801 cgtcggtaaa aagaatgccc ttaccggggg agaccatcca tggaattgat tttcatactt
     1861 tcttcggagg aaaaggagcg aatcaggcta tcatggcagc caaattgggc ggtcgggtga
     1921 cgatgatcgc aaaggtcggc aacgacggat tcgggcagca atatcttgaa aattatcaaa
     1981 gttcgggctt ggtgacggat tatgtttccg ttacggatga agcccccacc ggaatcgcgg
     2041 cgatcaccgt tgacgagcag ggacagaatt caatcatcgt tgtcagcggg gcaaatttcg
     2101 cgatgactcc gagcgatatt gaaaacgcca ctccggctgt tcgggaggca gacgcagccc
     2161 tttgccagtt ggagattccg ctggatgcga cgattcgatt tttagaaatt gcgaaggatg
     2221 cgaatgtctt gacgattttc aattcggctc cggcacaaga gctgccggaa aagatttatg
     2281 ctttaagtga ttatttttgt cccaatgaaa ccgaagcggc actgatggca ggatttccgg
     2341 taaaaacgat cgaagatgcc gaaaaggcgg gaagattatt catcgggaga ggtgcaaaat
     2401 ccgtaatcgt tactatgggc gggctgggat ctttattcgt ttccgcggat aaagttttcc
     2461 atattccggc tgaaaaggtg aaagcggtgg atacaacagg tgccggggat gcgtatatcg
     2521 gcagcattgc c
//
LOCUS       DAOMIV010000315         2525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_45417_length_2525_cov_4.851822, whole genome shotgun sequence.
ACCESSION   DAOMIV010000315 DAOMIV010000000
VERSION     DAOMIV010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45417_length_2525_cov_4.851822"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..>1357)
                     /locus_tag="PKV59_07405"
     CDS             complement(259..>1357)
                     /locus_tag="PKV59_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HQP46615.1"
                     /translation="NLSSKRLRNRKNISGVSMKNYVNGLNVGGWISQYFNKRYEHFDS
                     FIVEKDFEKIASWGFDHIRLPFDYEILENKENPFVFNERGFKYLDNAVLWSKKYGISL
                     LLDMHSAPGFSFGSYEVSTLFKNKTQQEIYIKLWETIALRYKNEKHIAFDLLNELVLP
                     SPDPWNNLVRECIASIRKVDTERLLIFGGINYNSIFTLKDIEIYDDENIAYTFHFYEP
                     IIFTHQKAVWMPFLVHYNQTLNYPGAFDNLDKLVSDVKDGKIGDIEQFDVKRYLTKEL
                     DISLMIEDMQPAIDFINKTGRKLYCGEFGVIESTPMRSRINWCRDAISLFDRYKIGRS
                     YWTYKGMDFGLLDRKGNLISEDLVKVLAGRR"
     assembly_gap    1358..1457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1472..>2525
                     /locus_tag="PKV59_07410"
     CDS             1472..>2525
                     /locus_tag="PKV59_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HQP46616.1"
                     /translation="MKISVNHLGFKPKSEKKAVIWGNDEYDSFSVINLTEMGYNEIGP
                     NTKPNTVIFSGKLEKNDYPWGKYSIADFSKLKTPGIYLISLNNKYNSVPFQIREDIYG
                     RTIRKPFDYIHAQRCGDHVPFYHSPCHLDDAVFRDNNKYCDTVGGWHDAGDLRKWTAH
                     TMMLGIALNHLKRINDPDWRVFDPAHGDISNELKWGNQYFLKAQSESGLVYHDVAGGV
                     EGDNSDNRWTNNIIGDGDDRHINTMHDGVVQWEFVYFESMNALTFAESDPYYSDICKK
                     AALKTYEYALTKELSKCEEIAWAVLALKELYSATGDDKILSELESKTKLLLSLQENEF
                     KFGQKNLRGFFYADTSK"
BASE COUNT          802 a          496 c          433 g          694 t
ORIGIN      
        1 tcgatgattt ttacttcatc aattactccg aaaacagatt catatttaat tggataaatg
       61 cggccaaatt cagcccaatg ttttagccag agagacttat acgcatttat tctttcaagt
      121 ccgggatgat aagttaatgc ctgttgattt tttgaagata tgtacacata gtcaatacgt
      181 ataaaaaaag ataatgatac ttttttattt tacaactaac cgcaaaagaa actttaccgg
      241 cataagccgg taaacctttt atcttcttcc ggcaagaacc tttacgagat cttcgctgat
      301 gagattacct tttctgtcaa gaagaccgaa gtccattccc ttgtatgtcc agtacgaacg
      361 cccgatttta tacctatcaa aaagcgatat ggcatcgcga caccagttaa tgcggcttct
      421 catcggagtc gattcgatta cgccgaattc accgcagtaa agctttcttc ctgttttatt
      481 gataaaatct atagcaggct gcatatcttc aatcatcagt gaaatatcaa gttccttcgt
      541 gagatatctc tttacatcaa actgttcgat gtcgccgatt tttccatctt ttacatcaga
      601 cacgagctta tcaagattat caaatgctcc tggataattc aatgtctgat tataatgaac
      661 cagaaaaggc atccatacag ctttttgatg agtgaaaata ataggttcgt aaaaatgaaa
      721 agtataggca atgttttcat catcatatat ttcaatatct ttaagtgtaa aaatcgagtt
      781 ataatttatt ccgccgaaaa tcaatagacg ttccgtatct acttttctga ttgaagcaat
      841 acattcacgt acaagattat tccaagggtc aggagacgga agtaccagct cattcaaaag
      901 atcaaaagca atatgttttt catttttata tcttaaagcg attgtctccc acagttttat
      961 ataaatttcc tgttgggtct tgtttttaaa aagagtgctg acttcataac ttccgaaact
     1021 gaatccggga gcggaatgca tatccaacaa aagagaaatt ccgtactttt ttgaccaaag
     1081 aaccgcgtta tcgagatatt taaatcccct ttcattaaat acaaaagggt tctccttgtt
     1141 ctcaaggatt tcataatcga aaggtagtct aatatggtca aaaccccatg aggctatttt
     1201 ttcaaaatcc ttctcgacaa taaaggaatc aaagtgttca tatcttttat tgaaatattg
     1261 tgaaatccac ccgcctacat tcagtccatt cacatagttt ttcatactaa cccccgaaat
     1321 atttttacga ttgcgcaaac gtttgcttga caaattannn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnncaa agggaggaaa aatgaaaata tcagtgaatc atctcggctt
     1501 caagccgaaa tcagaaaaga aagccgtcat ctggggaaat gatgaatacg attcattcag
     1561 tgtaataaat cttacagaaa tgggatacaa tgaaatcgga cccaacacaa aaccgaacac
     1621 agtcattttc tccggtaaac ttgaaaagaa tgattaccca tggggcaagt attcaattgc
     1681 ggatttctca aaactaaaaa ctcccggaat ctatcttatc tcactcaata acaaatataa
     1741 ttcagtacct tttcaaatca gagaagatat ttacggccgc acgatacgca aacctttcga
     1801 ttatatccat gctcaaagat gcggcgatca cgttccattt tatcacagtc cgtgccatct
     1861 tgatgacgca gtcttccgcg acaacaataa gtattgcgac acagtaggcg gctggcatga
     1921 tgcgggagat ttaaggaaat ggacagcaca tacgatgatg ctcggaatcg cactaaacca
     1981 tctcaaacgt ataaatgatc cagactggag agtttttgat cctgctcacg gcgatatttc
     2041 aaatgaactc aaatggggaa atcagtattt tctgaaagcg cagagtgaat caggtctggt
     2101 ctaccacgat gttgcaggag gagtcgaagg cgataattcc gacaaccggt ggacgaataa
     2161 cataatagga gacggagacg acagacacat caacacaatg catgacggag tcgttcagtg
     2221 ggaattcgtt tacttcgaat caatgaatgc actcaccttc gccgaaagcg atccgtatta
     2281 ttcggacatc tgtaaaaaag ctgcacttaa aacttacgag tatgcgctta ccaaagaatt
     2341 aagcaaatgc gaagaaatag catgggcagt tctcgcactg aaagaactat actcggcaac
     2401 cggagatgat aaaattcttt ccgagctcga aagcaaaaca aagcttctgc tttcacttca
     2461 ggaaaatgaa tttaaattcg gtcagaaaaa tcttcgcgga ttcttttacg ccgacacttc
     2521 caaaa
//
LOCUS       DAOMIV010000316         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_45468_length_2523_cov_5.155186, whole genome shotgun sequence.
ACCESSION   DAOMIV010000316 DAOMIV010000000
VERSION     DAOMIV010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45468_length_2523_cov_5.155186"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="PKV59_07415"
     CDS             <1..469
                     /locus_tag="PKV59_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP46617.1"
                     /translation="FRPWLGLEETALITCSLISVWQYIGTPMILFYTALIAIPDELME
                     AAYVDGANAWQIFWRVKFPLILPTVGLVAMMTFVGNFNAFDLIYTMQGALAGPNLSTD
                     IMGTLFYRTFFGQQQQMPNQTLGATIAAMQFIIILIGVVIYMIWQKRTTTYEY"
     gene            480..1358
                     /locus_tag="PKV59_07420"
     CDS             480..1358
                     /locus_tag="PKV59_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP46618.1"
                     /translation="MTATELNKKKGASVSYRIISWLRSAGPILFLIFWSIIALFPIYI
                     ILVNAFKNRNAIFKYPFNIPNAETFSLIGFETVNKRANFARYFTNSIVVTVVSLAITL
                     LIATMASFALSRYKFKGATLLSLIFSIGIMIPIRLATVGILRLMVNWSLTNTLLSLII
                     VYIAQSLPMAIFILSSFMKQIPQELIDAARVDGASEYRIYWMILPLIRPALGAIGIFS
                     MIPIWNDLWWPLILAPSAETATVTLGAQQFLGQYMTDWNAVLASLTMSIIPILIFYLI
                     FSKQMIKSITSGAIKS"
     gene            1386..1724
                     /locus_tag="PKV59_07425"
                     /pseudo
     CDS             1386..1724
                     /locus_tag="PKV59_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            1779..2396
                     /locus_tag="PKV59_07430"
     CDS             1779..2396
                     /locus_tag="PKV59_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP46619.1"
                     /translation="MVDSGQIGKPGTIHLRRCSYRPKKPSNNWFLDEQKSGGILMDLA
                     IHDFDYARWVAGDVRTVYTQKVSSLDPSSPIDFGLTILTHENGTLTHVTCGWAYPPPN
                     FQTGFDINCERGAVHFDSEETMPIHNLIQTDKDAPDVALPGSPLAESPYTSEIKEFYR
                     AICYGDEIRVTGMDGVKAVQIACAARESARTGEPVQIKPLEGVLK"
BASE COUNT          649 a          536 c          639 g          699 t
ORIGIN      
        1 tttccgacct tggctgggct tagaagaaac agcgttgatc acctgttcat taatctccgt
       61 ttggcagtat atcggaacgc ctatgatctt gttttatacc gcattaatcg ccatacccga
      121 tgaattaatg gaagcggcat atgtggatgg tgctaatgcc tggcagattt tttggcgggt
      181 aaaatttccg ttgatcttac cgacggtggg tttggtagcc atgatgacct ttgtcggaaa
      241 ctttaatgca ttcgatctga tctataccat gcaaggagcg ttggcggggc cgaatctttc
      301 gacggacatt atgggaactc tgttttatcg tacctttttc ggacagcaac agcaaatgcc
      361 caatcaaacg ctgggcgcga cgattgcagc gatgcagttc attattatcc tgatcggtgt
      421 tgttatttat atgatctggc aaaaacgcac caccacttat gaatattgag gaggaaaaaa
      481 tgaccgcaac cgagttaaac aagaaaaaag gcgcctctgt ctcttatcgg atcatttcat
      541 ggctgcgaag tgccgggcct attctatttt tgattttttg gagcattatc gcattattcc
      601 caatttatat tattttggtg aacgcgttta aaaatcggaa tgcgatattt aaatacccgt
      661 ttaatatccc caatgccgaa actttcagct taattggttt tgaaacagta aataaacgag
      721 cgaattttgc ccgatatttt accaacagta ttgtggtcac ggtagtatcg ttggcgatta
      781 ctttattaat tgccaccatg gcatctttcg ctttatcgcg ctataaattc aaaggagcta
      841 ctttattaag tctgatcttt tccatcggca ttatgatacc catccggctt gcgaccgtag
      901 ggattctgcg gctgatggtg aattggtcct taacaaatac gttgctttct ttgattatcg
      961 tttacatcgc acaatcttta ccgatggcga tctttattct ttcttcgttt atgaaacaga
     1021 ttccgcagga attaattgat gcggcaaggg tggacggagc cagcgaatac cggatttatt
     1081 ggatgatatt gcctctgata cgaccggcgc tgggggcaat cgggatattt tcgatgatac
     1141 cgatttggaa tgatttgtgg tggccgctga ttttggcacc gagtgcggaa acggcgacgg
     1201 taaccttggg cgcgcaacag ttccttggcc aatatatgac ggattggaat gcggtattgg
     1261 catcgttgac catgtcaatc attcccattc tgatctttta cctgatattc tccaaacaaa
     1321 tgatcaaatc gatcacatcg ggtgcgatca agtcataatc caatgaaaag acggaaagag
     1381 agggaacacg aatgaaagta ggaatagtcg gtaccggttc gatgggttcc acccatgcca
     1441 tggggtgggc ggaaacggat gcagaaattg tcggtttctg cgcggaatcc gttccggaag
     1501 ccgaaccgct cgccaagaaa taccatacaa aagtttttga gaagctggat caattgttgt
     1561 ccgaagcgga tattgttgat atttgcactc ccacttattt gcatccgact caggtgagac
     1621 aagcggccaa agcccgaaag cagattattt gtgagaaacc gttggcgttg gactggcaat
     1681 ccggattgga aatgctgagg gcttgcgagg aagccggagt cagactgttg gttgcgcagg
     1741 ttgtccgatt cttctccgaa tatgcggcgg ctaaagagtt ggtcgacagc ggccaaatcg
     1801 ggaaacccgg aacgattcat ttgcgccgct gcagttatcg tccgaaaaag ccctcgaata
     1861 attggttcct ggacgaacag aaatcgggcg gcattctgat ggacctggct atccatgatt
     1921 ttgattatgc aagatgggta gctggagatg ttcggacggt ctatactcag aaagtcagtt
     1981 ctttggatcc gtcctcaccg atcgatttcg gattgaccat tcttacccat gaaaacggga
     2041 cgctgaccca tgtcacttgt gggtgggcat atccccctcc gaatttccag acgggttttg
     2101 atatcaattg cgaacgggga gccgtccatt ttgattccga agaaaccatg ccgattcata
     2161 atctgattca aaccgataaa gatgctccgg atgtcgcatt accggggagc ccgctggcgg
     2221 aaagtcctta tacgagcgaa attaaggagt tttatcgggc gatttgttac ggagatgaga
     2281 tacgggtaac gggaatggac ggcgtcaaag ccgttcagat cgcttgcgcg gcgcgggaat
     2341 ccgcccggac cggagaaccg gtgcaaatca aaccgctgga aggagttttg aaatgattaa
     2401 gatcgggatc atgagttttg cacatttgca tgcggaagcc tatattcaga atattcgttc
     2461 aatcccggga gtagaatgga tcggtttcgt cgacccggac aacggtcgag tgaaacaatt
     2521 ttc
//
LOCUS       DAOMIV010000317         2517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_45624_length_2517_cov_4.045491, whole genome shotgun sequence.
ACCESSION   DAOMIV010000317 DAOMIV010000000
VERSION     DAOMIV010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45624_length_2517_cov_4.045491"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..361
                     /locus_tag="PKV59_07435"
     CDS             53..361
                     /locus_tag="PKV59_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46620.1"
                     /translation="MPVLFVVVEQWLRYKLALSVVVEQWLRYKPALFVVVEQWLRYKL
                     AAFVAEQWLRYKLALFVAEQWLRYKLALFVAERFEFVLRQQRLKRQKRLRLNFFPAAH
                     "
     gene            complement(335..1153)
                     /locus_tag="PKV59_07440"
     CDS             complement(335..1153)
                     /locus_tag="PKV59_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46621.1"
                     /translation="MNKKHRIYVSLLFFITVLLFSECLSVGAFYQEAAEPPYSGVNET
                     DWAALQAIYNKMTPYGQANSGWATGGNPCDPAWPGITCENGAVVKLDFEGRDFFCSIP
                     EEITQLSNLREIRMVNMGLRGIIPENLFGMQNLQTVNFSHNLLTGPLPQGSNQTLQTL
                     IIEDNKWTDQKQQELSARPDLSLCESVVQYPPEKNINMDPGLDGMIPSSLGYFPRLTK
                     LDLSGNMLEGEIPNDLINLNSLSSIDLSDNNPGNPLTISDQALADKLMRCRKKI"
     gene            1320..>2517
                     /locus_tag="PKV59_07445"
     CDS             1320..>2517
                     /locus_tag="PKV59_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46622.1"
                     /translation="MVASIRLSEAQNELITLPLSEHIFLDGKAGCGKTTAAIERLRKI
                     LNSPVSGSEVLVLLPSKTSSQKYYETYSVEGKRATVNTYNGLIQKAIKLFWPVVAEEA
                     GFASPEKSPIFLTIETSQILMAELIKPKIEAGYFSALRMPLSRIYNQIQIALHKCAAA
                     EFSFDSYAQRMRSSWSGDSAMLTVFDQVQECGNLFRKVCFENNLLDYSIQVEIFQNFL
                     LPSDRFQKWLKTNYHHLIYDNIEEDIPAAHRFAEILGESCESVLMVMDHLGGFRNFMG
                     SDTNSAFRLSEKCSVKRTFNDSFICEPAIRAMIHAIENPWIPTETLSANPKTAYIFQS
                     FHPYPVMIHQAAQDVAELINRQNVEPSDIAIIAPLLSDVLYTALERELRIQNISCYIH
                     RPSRPLI"
BASE COUNT          667 a          539 c          518 g          793 t
ORIGIN      
        1 aatatatctt cataggcttg ctgcgtctgc tgctgggcga tagcatcgtt atttgcctgt
       61 tctgtttgtt gttgttgagc aatggcttcg ttataagctt gctctgtctg ttgttgttga
      121 gcaatggctt cgttataagc ctgctctgtt tgttgttgtt gagcaatggc ttcgttataa
      181 gcttgctgcg tttgttgctg agcaatggct tcgttataag cttgctctgt ttgttgctga
      241 gcaatggctt cgttataagc ttgctctgtt tgttgctgag cgatttgagt ttgttcttcg
      301 tcaacagcgg ttgaaacggc aaaagagact ccgtctaaat ttttttccgg cagcgcatta
      361 atttatcggc taaagcctgg tcggaaattg ttaacggatt accgggatta ttatcactca
      421 gatcaatcga acttaaggaa ttcaggttga ttaaatcatt cgggatttcc ccttcaagca
      481 tatttcccga caaatccagt tttgtcaatc gaggaaaata acctaaggaa gaaggaatca
      541 ttccgtctaa accgggatcc atatttatat ttttttccgg cggatactga actacgcttt
      601 cacagaggga taagtccgga cgtgccgata attcttgttg tttttgatcg gtccatttat
      661 tatcttcgat aattaatgtc tgaagtgttt gattcgaacc ttgcggtaaa ggtcccgtca
      721 ataagttatg tgaaaagtta actgtttgaa gattttgcat gccgaacaga ttttcgggaa
      781 taatgccgcg gagtcccata ttcaccatcc gtatttccct taaatttgaa agttgtgtaa
      841 tttcttccgg aatagaacaa aagaaatctc ggccttcgaa atcgagttta actaccgcac
      901 cgttctcaca ggtaattccg ggccaagccg gatcacaagg atttccgccg gtagcccaac
      961 cggaatttgc ttgcccataa ggcgtcattt tattatagat agcttgtaaa gctgcccagt
     1021 ccgtctcatt tacgccgctg taaggcggtt cagcagcttc ctgataaaaa gcaccgacgc
     1081 ttaaacattc tgaaaatagc aataccgtga taaagaataa caaagagaca tagattctgt
     1141 gctttttgtt catttttccc tcttcttccg aatttcccac gagtttcttt cttccttcta
     1201 attctacctg acaaagattc gttattatag agaaacattt tctctctttt ttatacgttt
     1261 tttattcgtt aatgttccct cgcgaattcg agaaatttca aggtatactc aatttgatca
     1321 tggtggcatc aattcggtta tccgaagcac aaaatgaatt aatcacatta ccgctttccg
     1381 agcatatttt cttggacgga aaagcggggt gcggaaaaac aaccgcagcc atagaaaggc
     1441 tgcggaagat tttaaattcg ccggtcagcg gttccgaagt gttggtttta ctgccctcca
     1501 aaacatcttc ccaaaaatac tacgagactt actcagtcga gggaaaacga gcgacagtca
     1561 atacttacaa cggattgatt caaaaagcca ttaaattatt ctggccggtt gtggcggaag
     1621 aagcgggatt tgcttctccg gaaaaaagtc cgatttttct aaccatcgag acctctcaaa
     1681 ttcttatggc agagttgatt aaaccgaaaa tcgaagccgg ctatttcagc gcgctccgta
     1741 tgcctttgag tcgaatttat aaccaaattc aaatcgctct acataaatgt gcggcagcag
     1801 aattttcttt cgatagctat gcgcaaagaa tgcgatcgag ctggagtgga gattccgcca
     1861 tgttaactgt ttttgaccag gtacaggaat gcggaaatct ttttcgaaaa gtatgcttcg
     1921 aaaataatct cttggattat tcgattcaag ttgaaatctt tcagaatttt ctcctcccgt
     1981 ctgaccgatt ccaaaaatgg ctgaaaacaa attatcatca tctcatttat gacaacatag
     2041 aagaagatat ccccgcagct catcgattcg cggaaattct tggcgaatct tgcgaatcgg
     2101 tccttatggt catggatcat ttgggtggat tccgtaattt tatggggagt gatactaatt
     2161 ccgctttccg tctttccgaa aaatgttcgg taaaacgcac atttaatgac tcttttattt
     2221 gcgagccggc tatccgggcc atgatccatg cgattgaaaa cccgtggata ccgaccgaaa
     2281 cgctttccgc aaacccgaaa acagcttata tctttcaatc gtttcatccc tatccggtaa
     2341 tgattcatca ggcggcacag gatgtcgccg aattaatcaa ccgtcaaaat gttgaacctt
     2401 ccgacattgc gataatcgcc cctttgcttt cggatgtctt atataccgcg ttggagcgtg
     2461 agttgcgaat tcagaacata tcttgttata ttcatcgacc ttctcgccca ttaattc
//
LOCUS       DAOMIV010000318         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_46105_length_2499_cov_3.214812, whole genome shotgun sequence.
ACCESSION   DAOMIV010000318 DAOMIV010000000
VERSION     DAOMIV010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46105_length_2499_cov_3.214812"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..>2499
                     /locus_tag="PKV59_07450"
     CDS             191..>2499
                     /locus_tag="PKV59_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQP46623.1"
                     /translation="MNTLQTNSKNPASSKTATPDIERIYHYTNEEVLAMLESSPAGLS
                     SQQIKERQDRDGKNIIKEAKEKSILLVFLSNFVSVMAILLWVSGFIAILARMPELGIA
                     IWCVNLINGVFSFWQEYQAGKATEALKKMLPSYSRVIRDGTEQQILAEDLVVGDIILV
                     TEGDKISADARLLEATDLQVNQSTLTGESNPVRKVSDPIYRDDVTRSEIPNLIFAGTS
                     VSSGNGKAVVISTGMNTMFGNIAKLTQSMKEEKSPLQKELDKLTQQISAIALGFGVLF
                     FVLAVFFVRDPIAKSFIFSLGMVVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSA
                     VETLGCTSVICSDKTGTLTQNEMTVSNLYLFDRELEVSGTGYAPEGEIRYRGKAVTAD
                     EDEDLRLLLTAAGLCSNARLIEPNENSPQYTVLGDPTEACLGVVARKGGLDPKSLNEE
                     QPRIRELPFDSSRKRMTTIHEVDNRRVAYVKGAPKEVIELCSDRIQEGTKIPLTDSDR
                     EKLMAINDTYARRGLRVLAVAFRDLSESSSLPRAMSQYTPDLIEKNMTFVGLIAMADP
                     ARPEVAQAVAICHKASIRVIMITGDYGLTAESIGKRIGIVKQEHPRIVTGAELSRMSN
                     DDLKAALKDEVIFARVAPEQKYQVVSCLQEMGEIVAVTGDGVNDSPALKKADIGIAMG
                     ITGTDVAKEAADMILLDDNFASIVKAIEEGRTVYQNIRKFLTYILNSNMPEAVPSAFF
                     LFSRGLIPLPLTVMQILTIDLGTDMTPALG"
BASE COUNT          697 a          543 c          630 g          629 t
ORIGIN      
        1 atcgatacga caaaataacg gaaccaaagt cggcataatt atgccgactt tggtttttat
       61 tagaaatttg taaaggttat ctcatatttt ttgcacctca aatttaagga atatttaata
      121 gtgctataat ctagccgatt acggaaaggg ctgtcggaat tgttgttccc gtaattaaag
      181 gaataaattt ttgaacacct tacaaacgaa ttctaagaac ccggcgagtt ctaaaaccgc
      241 tactcccgat attgaaagaa tttatcacta tacgaatgaa gaagtgctgg cgatgctgga
      301 aagttcaccg gccgggctga gttcgcaaca gattaaagaa cgacaagatc gggacggaaa
      361 aaatatcatt aaagaagcca aagaaaaatc aattcttttg gtctttcttt cgaacttcgt
      421 cagcgtcatg gcaatacttt tatgggtcag cggatttatc gctatattgg cccggatgcc
      481 tgagctgggc atcgccattt ggtgcgtcaa tctgatcaac ggcgtgttca gtttttggca
      541 ggaatatcaa gccggaaaag caaccgaagc actcaaaaaa atgttgccgt cttactctcg
      601 tgtcatccgg gatggtaccg agcaacaaat attggccgaa gatttggtcg tcggagacat
      661 tattttggtc accgaaggcg ataaaatatc cgccgatgcc agattgttgg aagccaccga
      721 tctgcaggtc aatcaatcca ccttaaccgg agaatccaat ccggtgcgca aggtttcgga
      781 tccgatttat cgggacgatg tgacccgttc ggaaataccg aacctgattt tcgccggaac
      841 aagcgtctct tccggaaacg gaaaagccgt tgtgatcagt accggtatga atacgatgtt
      901 cggaaacatc gccaagttaa cccagtccat gaaggaagaa aaaagtccgc tgcaaaaaga
      961 gttggacaaa ctgacgcaac agatttcagc cattgccttg ggatttggcg tattgttttt
     1021 cgttttagct gttttcttcg tcagagatcc gatcgccaaa tcatttattt tttccctcgg
     1081 aatggtggtc gcatttatcc cggaagggtt gttaccgacg gtcacccttt ctctggctat
     1141 ggctgttcaa cgaatggcaa aagaacatgc gttggttaaa aagctttcgg ctgtcgaaac
     1201 gctgggttgt acctcggtga tttgctccga taagacgggg acactcaccc aaaatgaaat
     1261 gactgtcagt aatctatacc tttttgaccg cgagctggaa gtaagcggta ctggttatgc
     1321 accggaggga gagattcggt accgcggcaa agccgttacc gcggatgaag atgaagatct
     1381 tcgccttttg ttgaccgcgg ccggtctgtg cagcaacgcc cgcttaattg agccgaatga
     1441 aaacagtccg caatatactg ttttgggtga tcccacggaa gcctgtctgg gagttgtggc
     1501 gcgcaaaggc ggccttgatc caaaaagttt gaacgaagaa cagcctcgaa tacgtgaact
     1561 gccatttgat tcatcaagaa aaagaatgac tacgatccat gaggtcgata accggcgggt
     1621 tgcttatgtc aaaggggcac cgaaagaagt aatcgaactt tgctccgatc gaattcagga
     1681 agggacgaag atcccgctga ccgattcgga tcgggaaaag ttgatggcaa tcaatgacac
     1741 ttatgcgaga aggggcttac gtgttcttgc ggttgctttt cgggatttaa gtgaatcgag
     1801 ttcgctgccg cgagcgatga gccaatatac tcccgacttg atcgaaaaaa atatgacctt
     1861 tgtcggcttg atcgcaatgg ccgatcccgc caggccggaa gtggcgcaag ctgtcgcaat
     1921 ctgccacaaa gcttcgattc gtgttattat gatcaccgga gattacggat taaccgccga
     1981 gagtatcggt aaaagaatcg gtattgttaa gcaggagcat ccgaggattg taacgggcgc
     2041 cgaattaagc agaatgagta acgatgatct gaaagcggcg ctgaaagatg aagtgatttt
     2101 tgcccgggtt gcaccggaac aaaaatacca ggtggtgtct tgtcttcagg aaatgggaga
     2161 gatcgtcgcc gttacgggtg acggtgtcaa tgactcgcca gcattgaaaa aagcggatat
     2221 cggaattgca atgggtataa ccggtaccga tgtcgcaaaa gaagcggctg atatgatcct
     2281 gctggacgat aatttcgcct cgattgtaaa agcaatcgaa gagggacgta cggtttatca
     2341 aaatatccgt aaattcctga cgtatatcct aaacagtaac atgcccgaag ccgtaccatc
     2401 cgcctttttc ttgttctctc gcggattgat ccccttgccg ctgaccgtca tgcagatctt
     2461 aaccatcgat ctgggaacgg atatgacacc tgccctggg
//
LOCUS       DAOMIV010000319         2484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_46551_length_2484_cov_3.393166, whole genome shotgun sequence.
ACCESSION   DAOMIV010000319 DAOMIV010000000
VERSION     DAOMIV010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46551_length_2484_cov_3.393166"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PKV59_07455"
     CDS             <1..338
                     /locus_tag="PKV59_07455"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQP46624.1"
                     /translation="RTPTEQQEKDFQRISDAGAVVVSGSQAHFSQTMKPFDDRFIHYG
                     LGNLFFDQMDRPVVGTREEFLDRYVFYDGKLIQVELITALLEDYAQPVPMKADERQKF
                     LKEIFSYVK"
     gene            328..1137
                     /locus_tag="PKV59_07460"
     CDS             328..1137
                     /locus_tag="PKV59_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQP46625.1"
                     /translation="MSNKKIFLIIYNQLFLIFGMLCLGINVIRCSAEPVRKILPYKSA
                     MINYSEPIKFSIYLPESNDQTDKYPVLYLLHGQSQDEKIWDQMGIKEIADHLISTGSI
                     SPLVIVMPREEAYMEKISESDFGNRIFNELIPYVESNFPVLTDPASRAIGGISRGALW
                     AQKIAFNHYGTFGLLGQHSLPAAVFSDYVIYRIYADSEGKIPLMKIRIDSGTDDPYLK
                     GAFAFSKQLADVKAPYTLVVEPGGHDPEYWKEHLEDYLIWYADNLKNPADD"
     gene            complement(1198..>2484)
                     /locus_tag="PKV59_07465"
     CDS             complement(1198..>2484)
                     /locus_tag="PKV59_07465"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQP46626.1"
                     /translation="VYDAAFRQCGAVRVKTVEEMMDLCTGFVSLPPLNGNRVIIVTNS
                     GGPAVLAADMGEEVGLNMAEVNPKTVETLKSFLPPHCGFRNPIDLTVEGTEDGYRKTL
                     LTVLDEFDAAVAMNICPPYLDSMKLAKGVADAAKISGKPIAANFLPNQVVKESVAYLK
                     DQGIPNYVSGERAVQSLAKMEQYSSMIRDIREDMGFTETWESLKKRIGSETYKLPGER
                     QILEPEAMAWLRDNQIPTLKFGVAFSPDEAAKVCEDVGFPCVMKVVSPQILHKSEMGG
                     VIIGIRDAESAKQAFETIKKRAEGKDFQGVIIYPQISGAQEVLLGLVEDSQFGPVVAF
                     GLGGIYTEIFHDIVLNIAPVNTAQATRMIQSIKGIKILQGARGAKPYDLDALARTISR
                     FSELPFIYPEISEADLNPVFLLHEGLVVGDVRIIKK"
BASE COUNT          704 a          557 c          513 g          710 t
ORIGIN      
        1 cccggactcc taccgaacaa caagaaaaag attttcaacg catttccgat gcaggtgccg
       61 tcgttgtctc cggatctcag gcacactttt cacagactat gaaaccgttt gacgatcggt
      121 ttatacacta cggtctggga aatttgtttt ttgatcaaat ggaccggccg gtagtcggga
      181 ccagggagga atttttagat cgatatgttt tctatgacgg aaaattgatc caagtcgaat
      241 taatcaccgc attgctggaa gattatgcac aacctgttcc gatgaaggct gatgaaagac
      301 agaagttttt aaaagagatt ttttcatatg tcaaataaga aaatatttct tattatttat
      361 aatcaattat tcttaatatt cggaatgctt tgcctgggta ttaatgttat tcgttgttcg
      421 gctgaaccgg ttcgaaaaat acttccctat aagagtgcga tgatcaacta ttccgagccg
      481 attaaatttt ctatctattt gcccgaatcg aatgatcaaa cggataaata tccggtcctt
      541 tatctcctcc atggacaaag ccaagatgaa aaaatatggg accaaatggg aattaaagaa
      601 atcgccgatc atttaatttc gaccggaagc atatctccgt tggtaattgt tatgccgcgt
      661 gaagaagctt atatggaaaa aatttccgaa tcggactttg ggaaccggat cttcaatgaa
      721 ctaatcccct atgtggaatc caattttccg gtgcttaccg atccggcttc aagagcaatc
      781 ggggggatat cacgcggggc tttatgggcg caaaagattg cgtttaatca ttacggtacc
      841 ttcggacttt taggacaaca cagtttaccg gcagctgttt tctcggatta tgtaatatat
      901 cgaatctatg ccgattccga aggaaagatc cccttaatga aaatacgaat cgacagcgga
      961 accgacgatc cttatctcaa gggggcgttc gctttttcca agcaattagc cgatgttaaa
     1021 gctccttata cgcttgttgt tgagccggga gggcatgatc cagaatattg gaaagaacat
     1081 ttggaggatt atctgatttg gtatgcggat aatttgaaaa atcctgcaga cgattgagac
     1141 gaacagaaaa agcttgagaa atttctcaag ctttttcatc aacaaaatat aaaaaaatca
     1201 ctttttaata attcgtacgt ctccgacgac caaaccttca tgcaacaaga acaccggatt
     1261 tagatcagct tccgaaattt ccggataaat gaacggcaat tcggaaaaac gagaaatggt
     1321 ccgggcaagt gcatccaagt catatggctt tgctcctctg gcgccctgta aaatcttaat
     1381 tcccttgatc gattgaatca tacgagtcgc ttgagcggta tttacgggag cgatattcaa
     1441 aacaatatca tggaaaatct cggtataaat tcctcccaaa ccaaatgcca caacggggcc
     1501 gaattgggaa tcttccacca atcccagcaa tacttcctga gcaccagaaa tttgcggata
     1561 gataataaca ccttgaaaat cttttccttc ggcgcgtttt tttattgttt caaaggcttg
     1621 ttttgccgat tctgcgtcac gaatgccgat aatcacgccg cccatttccg atttgtgcaa
     1681 tatttgcggt gaaacaactt tcatgacaca tgggaaaccg acgtcttcac atactttcgc
     1741 agcctcatca ggagaaaagg cgacaccgaa ttttagcgtg ggaatttggt tatctctcaa
     1801 ccaagccatt gcttccggtt caaggatttg ccgctctccg ggtagtttat aggtttcgga
     1861 tccgattcgt tttttcaaag actcccatgt ttcggtaaaa cccatatctt cccgaatgtc
     1921 acggatcatt gaagaatatt gttccatttt tgcgagcgat tgaaccgctc gttctccgct
     1981 gacataattg gggatacctt ggtcttttaa ataagcaacg ctctctttaa ctacctgatt
     2041 cggtaaaaaa tttgccgcaa tcggtttacc gctgattttt gccgcatcgg ccacaccttt
     2101 tgccagtttc attgaatcca aataaggcgg acagatattc attgcgacag cggcatcaaa
     2161 ctcatccaga accgttaaca atgttttccg atatccatct tcggtccctt caaccgttaa
     2221 gtcgatcgga tttcgaaaac cacaatgcgg cggaaggaat gatttgagcg tttcgacggt
     2281 tttcggattg acttcggcca tattcagtcc gacttcttcg cccatatcgg ctgctaaaac
     2341 agccggtccg ccggagtttg tgacgataat gacgcgattc ccattcagcg gcggcaaact
     2401 cacaaatccg gtgcatagat ccatcatttc ttcaaccgtc ttcactcgaa cagctccgca
     2461 ttgtctgaac gcagcatcgt aaac
//
LOCUS       DAOMIV010000320         2456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_47328_length_2456_cov_2.863807, whole genome shotgun sequence.
ACCESSION   DAOMIV010000320 DAOMIV010000000
VERSION     DAOMIV010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47328_length_2456_cov_2.863807"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1421
                     /locus_tag="PKV59_07470"
                     /pseudo
     CDS             216..1421
                     /locus_tag="PKV59_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            2283..2456
                     /locus_tag="PKV59_07475"
                     /pseudo
     CDS             2283..2456
                     /locus_tag="PKV59_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
BASE COUNT          750 a          460 c          476 g          770 t
ORIGIN      
        1 tattaccact ttctatgact ggaatgccga aattcaaaca cttgaaatgg ctcaatagac
       61 caaacaaacg gatgagatct gctttaaggc gttttattct caaaaacgaa tcagggggtg
      121 gtgcgtctta caccacccct aattttcaat gggagaaaaa taaaaattga aatattaatc
      181 atttcaaatt caggttcatc ggagcaaaaa ccttcatgtt taaatatttc ttgcgcagat
      241 tgacttatgc agtttttatt ctattttttg ggtcattgat tgtttatacc gtgatccgct
      301 ttctcccgac atcctacgtg gaaaatattg ccagacagcg atcaaattta cccggggcga
      361 aaagttattc acagattctc gcgaatctaa attctgtata cggcttggac accgatattt
      421 ttaccggttt cttaagatgg ggaaaaaacg caatccacgg agactttggt tattcttggc
      481 aatataatat tccggttgtt caaaaattca aagaagtcat ttggtactct gttttcttaa
      541 atgtggttac gcttctcgtg gaaacattca tctctattcc cctcgggatt ttggctgcca
      601 gaaaacagta cagtaagaca gattatgccg tgacggttct ttccatgatt tgtatttctt
      661 taccgacttt ctttctggct accctattaa aatatatatt ttccgtcaaa ttaggctggt
      721 tcgatttata cgggattgtc ggccggtact acgagcaact tgatgcatgg ggaaaatttc
      781 tggatatggc aaaacatctt gtcttgccgg tactcaccct ctcaatgttg aatatcggcg
      841 gattaatgcg gtatacccgt accaatatgc tggaggtctt gaacaccgat tacattcgta
      901 ctgctcgcgc aaaaggactt tccgaagatg tcgttgtcaa aaaacatgct tttcgaaata
      961 ctttggtccc cctggtcagt tatttttcat acctaatccc caatatgttt tccggttcgt
     1021 ttattacgga gacattgttt caaatccccg gtatcggata tatttcctat tatgcggtga
     1081 tccatggcga tattccgttc gctatgtttt attcggtttt aataattttt ctgacacaaa
     1141 tcagcttatt gatcgcggat tttatgtatg cggtggtaga tccccgtgtt cgaattaatt
     1201 tatggagacc gaaaatgaac caaaatcaaa caggaaatag acaaaccaat agtccgcaaa
     1261 gtgaagaaat tcggttggat gacgaaaaga ggatcaaagt actgtcaccg acaatgttgg
     1321 tttttaaacg gttcttacgg aacaaacttg caattgtcgg tttggttatt cttattttaa
     1381 tgttcctttt ctcttttctg gggggtattc tcagtcccta tcaacagcag caggttttcc
     1441 gtaaatatga aatgattccg aaagaatatg catctgccac aatcaatcaa aatctgcggt
     1501 atattgttca agatggggag attttcccta attccgcaat cgctaaattt gttttggcgc
     1561 tcaatgaagg acaaacaaga ttcaattcag aggaaacaga atattcgtat aaaaaagaag
     1621 gcgatgattt ttatctgata tcggaaaata agaaaatcgc cgatgtcatt attcttgccg
     1681 gtaaaggtcg atttacactt tcacaaggaa ttgattttcc agaagagtca caaaaactag
     1741 ccatagaagc gattcaagcc ggaaaaaatg aatttgaaag caatggaata aaatatttcc
     1801 tttccagaaa aggaaagata acgaccattt caatgccgaa agaaattgcg attgcgacga
     1861 aattaatttt tgatgctttc gaaaatgatg cgattccttc gttgaatcgg attgaattcg
     1921 tttatcaagc acaaaatgcc ttaataaata acatcgaacg attcagcgta aacggagaaa
     1981 attatcgaat agaaatttcg gaagattcat cgattattta taaggatgac acaaacggag
     2041 aaagaccttt cgcttcgatt tcgaatttta tcgtacaacc aaaatcacct gatgttttct
     2101 taacagtcga ttttaaaaag aaatttaatg aagcaattct ttcaaatcaa aattcatttt
     2161 ccgttttatc cgattccggg gaggaaattc cttataccat taaccgtgtg aatgaaactt
     2221 acaccatcta tactcagaca aaagcccaat tgattgatat gtacagccct ccatccaaag
     2281 aacattggct tggaaccgat gccaacggga tggatgttct gacgcgatta atgtatggag
     2341 gaagaatatc attaatggtc ggctttgtcg tcgtttttct tgaattattc atcggagtaa
     2401 tcgttggggg gatcgcaggc tattttggaa ataccgttga cacattgctg atgaga
//
LOCUS       DAOMIV010000321         2442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_47743_length_2442_cov_3.743611, whole genome shotgun sequence.
ACCESSION   DAOMIV010000321 DAOMIV010000000
VERSION     DAOMIV010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47743_length_2442_cov_3.743611"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /gene="lepA"
                     /locus_tag="PKV59_07480"
     CDS             complement(<1..1742)
                     /gene="lepA"
                     /locus_tag="PKV59_07480"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQP46627.1"
                     /translation="MKEHIRNFCIIAHVDHGKSTLADRLLQLTNTVPDRQMMDQILDS
                     MDLEREKGVTIKASAVRMNYNAKDGRDYELNLIDTPGHVDFSYEVSRALAACEGAILI
                     VDATQGIEAQTLANLYMALESDLVIIPVINKIDLPSADPDKTAKEISDLLGVDPDEIP
                     RISAKEGWNVDQVLELIVKMIPPPQGDDSKPTQALIFDSEYDSYKGVIAYVRVREGII
                     SPNMTLRMMATKADLKPVEIGIFSPAMKPVQQLTAGEVGYIATGLKTVKECRVGDTIT
                     SALEPAAEMLPGYKKAKPMVFAGIYPVEGEDYDDLRNALEKLQLNDASLDFQPETSQA
                     LNFGFRCGFLGLFHMEIVQERLEREYDLDIVVTAPSVEYQVELLNGTVITIDSPVQLP
                     DESTIKEIREPWMKLEIFSPVEFYGTIMDLARKRRGIFVSEEYPAPDRVQMNFEIPLA
                     ELIVNFFDELKSRTRGYASMDYQMYEYRPGNLVKVEVLVGGEPVDALATIVHKEDAYH
                     KGQLLVTKLKSLIPRQMFDVAIQASASGRIISRANVKALRKDVLAKCYGGDITRKRKL
                     LEKQKRGKKRMKMVG"
     gene            2031..>2442
                     /locus_tag="PKV59_07485"
     CDS             2031..>2442
                     /locus_tag="PKV59_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQP46628.1"
                     /translation="MKKSLSLSVAIFLFATLVIGIVSVQADGIKTLVVGFDQDFPPYG
                     YIGEDGNFTGYDLELAKAAADIMGLEIVYQPISWDSKDLELDSGTIDCIWNGFTINER
                     EDKYTWTEAYKGAGQVFLVSADSDIKTFDDLKDKV"
BASE COUNT          651 a          568 c          500 g          723 t
ORIGIN      
        1 cccaccatct tcatccgttt tttcccgcgc ttctgttttt ccaacaattt tcttttacgg
       61 gtaatatcgc ccccgtagca ttttgccaat acatcctttc gcagcgcttt gacattcgcc
      121 cgcgaaataa tcctgccgga agcacttgcc tgaatcgcca catcaaacat ttgccgcggt
      181 attaatgact ttaatttcgt gaccagtaac tgccctttgt ggtaagcatc ttctttatgg
      241 acgatcgtcg caagcgcatc caccggttct ccgcccacca aaacttccac tttgactaaa
      301 tttcccggtc tgtattcata catttgataa tccatcgaag cgtaaccccg agttcgggat
      361 ttcagttcat caaaaaaatt tacgattaat tcagccaaag ggatttcaaa attcatctgc
      421 acacgatcgg gagccggata ttcttcactg acaaatatgc cgcgtctttt tcgagccaaa
      481 tccataatcg tgccgtaaaa ttccaccgga gaaaagatct caagcttcat ccacggttcc
      541 cgaatttctt ttatggttga ctcatccggt aattgaacgg gggaatcaat cgtaataacc
      601 gttccgttta ataattccac ctgatattcc accgaaggag cggtgacgac aatatcaaga
      661 tcgtactccc gttccaatcg ttcctgcaca atttccatgt gaaataagcc caaaaatccg
      721 caacgaaagc cgaaattgag agcctgcgac gtctccggct gaaaatctaa cgacgcatca
      781 tttagttgaa gcttttccag cgcatttcgt aaatcgtcat aatcttctcc ctcaacggga
      841 taaattccgg caaagaccat cggcttggct tttttataac cggggagcat ttcggcggcc
      901 ggttcgagtg cggatgtaat agtatcacct actctgcatt cttttacggt tttcaatccc
      961 gtagcgatat atcccacttc ccctgcagtc agctgctgta cgggtttcat cgccggagaa
     1021 aaaatcccga tctcaacggg ttttaaatcg gctttggtcg ccatcatccg taaggtcata
     1081 ttgggcgata taatcccttc tctgacacga acataggcaa tcacaccttt ataagaatca
     1141 tattcggaat caaaaatcag agcttgagtg ggctttgagt cgtcaccttg aggcggcgga
     1201 atcattttga cgattaattc caacacttgg tctacgttcc agccttcttt cgctgaaatg
     1261 cgcgggattt catccggatc gacacccaac agatcggata tctcttttgc ggtcttatcg
     1321 gggtcggcag aaggcaggtc aatcttattg attaccggga taatcaccaa gtccgattcg
     1381 agagccatat acagatttgc aagtgtttgc gcttcgatcc cctgtgtcgc atccacaatc
     1441 agaattgcac cttcgcaagc cgccaacgcg cggctgactt cataactgaa gtcgacatgc
     1501 cccggtgtat cgatcagatt gagttcatag tctcggccgt ctttggcgtt atagttcatc
     1561 cggacagcgg aagctttaat cgtcacgcct ttctctcgtt ccagatccat ggaatccaag
     1621 atttgatcca tcatttgtcg atccggtact gtattggtta actgcaataa tcgatcagcc
     1681 agagttgatt ttccgtgatc cacgtgtgcg atgatgcaga aatttcggat atgttctttc
     1741 ataatcggga attaagtata tcatcgaaaa aattgttgaa tgacggggta ttcataaagt
     1801 atcctgtctc cgctgctttt atatcgctta aaaacaaatg agaataacct gaatatttga
     1861 atttacaact tatgaacgca gaataaaaat ggatcttgat cctttttata gattgaattt
     1921 acggacgatc ttttaattta tgtcacattt tttcaaatac tttattatgc taacgtggta
     1981 gagtgataaa ctatcggaag tttttgacaa ttctatttag gagaagcttt atgaaaaagt
     2041 ctttatcgtt gtctgttgca atctttttat ttgcaacctt ggtaatcgga atcgtttcgg
     2101 ttcaggctga tgggatcaaa acattggtcg tcggttttga tcaggatttt ccaccttacg
     2161 gctatatcgg cgaagacgga aacttcacgg gatatgatct tgaattagcg aaagccgccg
     2221 cggacataat ggggcttgag attgtgtatc aaccgatttc atgggattcc aaagatctgg
     2281 aactggactc cggaaccatc gattgtattt ggaatgggtt caccataaat gagcgcgaag
     2341 ataaatacac ctggaccgaa gcatataaag gcgccggaca agtcttttta gtttcggcag
     2401 attcggacat taaaactttt gacgacctga aagataaagt tg
//
LOCUS       DAOMIV010000322         2426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_48201_length_2426_cov_5.404471, whole genome shotgun sequence.
ACCESSION   DAOMIV010000322 DAOMIV010000000
VERSION     DAOMIV010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48201_length_2426_cov_5.404471"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1756)
                     /locus_tag="PKV59_07490"
     CDS             complement(<1..1756)
                     /locus_tag="PKV59_07490"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HQP46629.1"
                     /translation="MIPRLLKISGFLSYKEPVEIDFTRIHVACISGSNGAGKSALLDA
                     MTWALFGQARKNDETIINNSRNIDAAEVTFEFEYESNIYRIIRCLRRGKTSTVDLLIW
                     NQETGNWHPMTEKSVRQTNKRIEELLHLDFETFINASFFLQGKADQFTSQSAGDRKKI
                     LSNILELDIWEKYQTAAAEKRRNQTVRLSFLENRLDEIEKELSEEPRRREVLNSLDEE
                     LKKSEEQLVIKQQAWQNAKSAETLLESHQAAYQSLLQNYEKSARQIDAQKSRLSKRTL
                     ESLSIQELLKEEPEIESGYRQLQKIREELSEWNKKALENYRIDGERKEITGKIGQLRA
                     GLESEQATLRQEILRLEKNFELIPELSQKQKELQTQLESIEAFLSEKESVNESLRNIT
                     EIIVEKRAENQQLDAQMRDLKKRITELQAAQVGTPCPFCGRELDEEHTLTYLQSMQLD
                     GKKLGDRYRINSEELKKLNEEKDALNRKLSEIQNAEKKHLELSRLLAPISAEIKKIQE
                     NEPYRQKLSDRLNEIQKLLETESFCAEERQRLSVLDEQAKALGYNPAAHEQCREKESE
                     LALNENRWQKVQQAKAALQ"
     gene            complement(1797..>2426)
                     /locus_tag="PKV59_07495"
     CDS             complement(1797..>2426)
                     /locus_tag="PKV59_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQP46630.1"
                     /translation="SLAEIKESDDSYFPLVRGTTDNVVGIVSAKKILDAYISGDEIDL
                     SKMAETPLFIPESKPALSVVDDLRESGKQVAIVLDEYGGFSGMVTLLDILEELVGDVP
                     GLYDTYHPEIVEREDGSWLIDGQMDIEEFKDELGIKQLADEDRVGYQTLAGFMLSKFG
                     FIPKAGDSFEADGYRFEIVDMDNRRIDKVMVTKLASPSEDDSDKTDSEE"
BASE COUNT          567 a          570 c          483 g          806 t
ORIGIN      
        1 gctgtaaagc ggcttttgcc tgctgtactt tctgccagcg gttttcgttc agagctaatt
       61 cggattcttt ttcccgacat tgctcatggg ccgcgggatt atatccgaga gcttttgcct
      121 gttcgtcgag cacagataac ctttgccgct cttccgcgca gaaagattcc gtttccaaca
      181 atttttgaat ttcgttcaag cgatcggata atttttggcg ataaggttca ttttcctgaa
      241 tttttttgat ctcggcggaa atcggtgcga gtaaacgtga aagctcgaga tgcttttttt
      301 cagcattttg gatttccgat aatttccgat taagggcatc tttttcctca tttagctttt
      361 tcagttcttc gctattaatt cggtatcgat ctcccaactt tttcccgtca agctgcatgc
      421 tttgtaaata ggtgagagta tgttcctcat ccaattccct tccgcaaaaa gggcaagggg
      481 tccccacttg agctgcctga agttccgtta ttcgcttctt taaatcccgc atttgggcat
      541 ccagttgttg attctccgcc cgtttttcta cgatgatttc cgtaatgttc cgcagagatt
      601 cattcacaga ttctttttcg gaaaggaatg cttcaatact ttcaagttgt gtttgaagct
      661 ctttttgttt ttgggataat tccggtatta attcgaaatt tttttccagc ctgagaattt
      721 cttgtcgaag tgtggcttgt tcggattcca aaccggcacg caattgaccg attttacctg
      781 ttatctcttt tcgctctccg tcgatccgat aattttccag cgcttttttg ttccattcgg
      841 ataattcttc ccgaattttc tgtaattgcc ggtatcccga ctcgatttcc ggctcctctt
      901 tcagcaattc ctgaatagac aaagattcga gagttcgttt cgacaaccga cttttttgtg
      961 catcgatttg cctggcactc ttttcgtaat tctgcagaag agactgataa gcggcttgat
     1021 gggattccaa aagcgtttcg gcggattttg cattttgcca ggcttgttgt ttgatcacca
     1081 actgctcttc ggactttttc aattcttcgt ccaagctgtt caaaacttct ctgcgccggg
     1141 gttcttcgga taattctttt tcgatttcat ccagtcgatt ctcaagaaaa gataatctca
     1201 cagtctgatt gcggcgtttt tcggctgcgg cagtttggta tttttcccaa atatccaatt
     1261 ctaagatgtt ggaaagtatt ttttttcgat caccggcgct ttgacttgta aattgatccg
     1321 cttttccttg caaaaaaaag gaagcattga tgaatgtctc aaaatcgagg tgcagcaact
     1381 cttcgattct tttatttgtt tggcgaacgg atttttcggt catcggatgc cagtttcccg
     1441 tttcctgatt ccaaatcaac aaatcgacag tggaagtttt tccgcgtctg agacagcgaa
     1501 tgattcgata aatgtttgat tcatattcga actcaaatgt cacttccgct gcatcgatat
     1561 ttctggaatt attgataatc gtctcgtcat ttttccgagc ttgaccgaac aaagcccaag
     1621 tcatcgcatc caaaagggcg gattttccgg cgccgtttga accggaaatg caggcaacat
     1681 ggatccgggt aaagtcaatc tccaccggtt ctttatagga aagaaaaccg ctgattttca
     1741 gcagacgtgg aatcattttt tatcctttgg gaaaatttca catttcatga tttgacttat
     1801 tcttcggaat cggtcttatc ggagtcatct tcggatgggg aagctaattt ggtaaccatc
     1861 actttatcga ttcgacgatt atccatatcc acaatttcaa atcgatagcc gtccgcttcg
     1921 aaagaatccc cggctttggg gataaagccg aatttgctta acataaaccc ggccagcgtc
     1981 tgatagccga ctcgatcttc gtcagcgagt tgtttaattc cgagttcatc tttaaactct
     2041 tcaatatcca tttgaccgtc aatcagccat gaaccgtctt ccctttcgac aatttcggga
     2101 tgataggtat catataaacc gggaacgtct ccgaccagtt cttccaaaat atccaacaag
     2161 gtcaccatgc cggaaaatcc gccgtactca tccaaaacaa tcgccacctg cttgccggat
     2221 tccctcaaat catccacgac ggacagtgcc ggtttgcttt cgggaataaa cagaggggtt
     2281 tctgccattt tgctgagatc aatttcatca ccgctgatat atgcatccaa aatttttttc
     2341 gccgagacaa ttccgaccac gttatcggtt gttccccgaa ccaaaggaaa ataactgtca
     2401 tccgattcct taatttccgc caggct
//
LOCUS       DAOMIV010000323         2422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_48337_length_2422_cov_3.755809, whole genome shotgun sequence.
ACCESSION   DAOMIV010000323 DAOMIV010000000
VERSION     DAOMIV010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48337_length_2422_cov_3.755809"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="PKV59_07500"
     CDS             <1..975
                     /locus_tag="PKV59_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQP46631.1"
                     /translation="KSTRSTRYTADQIAKVLYWLSIPTNNERCRRLFSSLYHHAAEGD
                     FYLSLDHGQIATTKIKSIENLEDFFYPNSNRQFEELILSWELPEIVIHSLFSFRHLLK
                     KWLEARTLRIDQLILLIAQDLYSDPDSLGIASQLSRIALQMTQADPSMQLNAVAENIN
                     KAAANADLYPELSKPDIQFDPNLYQGKIVVTTFHKAKGLEWDQVYLTSCNNYEFPAGS
                     GYTPRGYREYIRSQFFYIRDQLDIQAESLEQLRILFNAEPSEIYREGKGSVQSYNSVV
                     SERLRLLYVGITRAKKGLHISWNSGHSEQGQEALAIKFLRECSGGKKK"
     gene            972..1757
                     /locus_tag="PKV59_07505"
     CDS             972..1757
                     /locus_tag="PKV59_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQP46632.1"
                     /translation="MKLISQSQIRDFQTCPQKYYLSHILKLSWPAPPSPLFKKLEEEA
                     ALGETFHLFVHRIIQGLNPEESAFETENKTIAKWIDNFLRNYPLPQDAVLYSERELTN
                     LDEDILWIGKFDVIATLQNKILIFDWKTSQKAGKVLWLEESPQTRLYRFLLYQNRQLY
                     SYDGTDHMKPEQIEMIYWFVNFPNSPIHIGYSEKQFAADRQFLKEIAAKINSDKAENY
                     PKTSIEKACNYCQFQTYCKRGFPDQSDSEYEPDWNNLPLTEEL"
     gene            complement(1825..2175)
                     /gene="rplT"
                     /locus_tag="PKV59_07510"
     CDS             complement(1825..2175)
                     /gene="rplT"
                     /locus_tag="PKV59_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQP46633.1"
                     /translation="MARVKSGPYGHQRHKRLLKRTEGYYATRRKLYRRANETLLKGMW
                     YAYRDRRVRKRELRKLWIARINAAARINGTTYSRLIAGLKKADINLNRKMLADIAVRD
                     PQTFSKIVEQAKVN"
BASE COUNT          706 a          546 c          491 g          679 t
ORIGIN      
        1 aagagtaccc ggagcactcg atataccgcc gatcaaatag caaaagtttt gtattggctt
       61 tccattccga ccaataacga acgctgcaga agattgtttt catctcttta tcaccacgcc
      121 gcggaaggcg atttttattt gagccttgat cacggtcaaa ttgcaacaac taagataaaa
      181 tcaattgaaa atcttgaaga cttcttttat ccgaattcga atcggcaatt tgaagaatta
      241 atcttatcct gggaactacc tgaaatcgtc attcattccc ttttctcatt ccgtcatcta
      301 ttaaaaaaat ggctggaagc aaggactctg cgcatcgatc aattgatttt attaatcgca
      361 caagacctgt attccgatcc cgattcgctc ggtatcgcaa gtcaattatc ccgaatcgca
      421 cttcaaatga ctcaggcaga tccgtccatg caattaaatg cggtggctga aaatatcaac
      481 aaagcagccg ccaacgctga tctctatccg gaattgagta agcctgatat tcagtttgac
      541 cccaatcttt atcaagggaa aatagttgtt accacctttc ataaagcaaa agggcttgaa
      601 tgggatcaag tttatttgac cagctgtaat aactatgaat ttcctgcggg aagcggttat
      661 acgccgagag gctatcgtga atacatccgg tcccagttct tttatattcg cgatcaactg
      721 gatattcaag cggaatcatt ggagcagctt cgcatactat ttaatgccga accctccgaa
      781 atttatcggg aaggaaaagg cagtgttcaa tcctataatt ccgtcgtatc cgaacgcctt
      841 cgcttgttat atgtcggtat tacacgtgca aaaaaaggat tgcatatttc atggaattcg
      901 ggacattccg aacagggaca ggaagctttg gcaataaaat ttctgcggga atgttcaggg
      961 ggaaaaaaga aatgaaatta atcagccaat cacaaattcg agactttcaa acctgcccgc
     1021 aaaaatatta tttgagccat atactgaaac tcagctggcc ggcgccgccc tcgcctcttt
     1081 ttaaaaagct cgaggaagaa gcagctttgg gggaaacctt tcatttattt gtacaccgga
     1141 tcattcaggg gctgaacccg gaagaatcag cgtttgaaac ggaaaataaa accattgcaa
     1201 aatggatcga taatttcctg cggaattacc cgttaccgca agacgctgtt ctttattcgg
     1261 agagggagct gacaaacctt gatgaagata ttctctggat cggtaaattt gacgttatcg
     1321 cgacattgca gaataaaata ctgatttttg attggaaaac atcacaaaaa gcagggaaag
     1381 ttttatggtt ggaagaaagt ccgcagactc gtctttatcg atttcttttg tatcaaaatc
     1441 gacagcttta ttcttacgac gggacggatc atatgaagcc cgaacaaatc gaaatgattt
     1501 attggtttgt taattttccg aattccccta tccacatcgg gtactcagaa aaacagttcg
     1561 cggcggaccg gcagttcttg aaagagattg ctgcaaaaat aaacagcgat aaagcggaaa
     1621 actatccgaa gacatccatc gaaaaggcgt gcaattattg ccaatttcaa acctattgca
     1681 aaaggggctt cccggatcaa tcagattcgg aatatgagcc ggattggaat aatctgccgc
     1741 tgacggaaga attataaaat aagacagctg ctaaatcggc agctgtctta ttttatatcg
     1801 agagaattct ttgatgtatt gattttaatt gactttcgcc tgctcgacga ttttgctgaa
     1861 agtctgcgga tcacgaaccg cgatatccgc cagcatcttc cgattcaggt tgatatccgc
     1921 ttttttcaat ccggcaatca atcggctata agtcgttccg tttattcggg cagccgcatt
     1981 aattcttgca atccaaagtt tacgcaattc ccgtttccgt actcgacgat cacgatatgc
     2041 ataccacatc cctttcagga gtgtttcatt tgcacgtctg tagagctttc tacgcgttgc
     2101 ataataacct tcggttcgtt tcaataaccg cttgtggcgc tggtgtccgt aaggaccact
     2161 ctttactctt gccatacgat aaattactcc tttgcgatcc cccgggcgtc gatcttttca
     2221 gatccgttgt gtacacccga cagccgcact aatcaaaaaa caattatttc tccatataag
     2281 gagcgagatg cttcacccga tcgataatgc tcttaccttg aacgggaatc atctcatcca
     2341 atagtgcttt tgtccgtttg gaagttcggc ggcgaaaatg gctttttccg ccctttgtac
     2401 gaaccaaaac accaccaccc gt
//
LOCUS       DAOMIV010000324         2420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_48381_length_2420_cov_5.996617, whole genome shotgun sequence.
ACCESSION   DAOMIV010000324 DAOMIV010000000
VERSION     DAOMIV010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48381_length_2420_cov_5.996617"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PKV59_07515"
     CDS             <1..1187
                     /locus_tag="PKV59_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46634.1"
                     /translation="RKPYSQQQTALGILDGFAQETINGQKVVKVFTREEIVKDEFKYL
                     NDDLRRKQIVAQFRSSIMGPVTHNLSNFSYALTTGIGSILCITQGFDIGGLTVFVNYT
                     RQFSRPINEISMQMNTVFAALAGAERVFELLDEPPEPPDAPDCVNVDHLAGHVVMSHV
                     NFGYYPDKLVLHDISLYAKPGQKIAFVGSTGAGKTTITNLLTRFYDIQSGEITIDGIP
                     IDKIQRKILRRNIAMVLQDTHLFTGTVRENIRYGRLDATDDDVIAAAKVANAHSFIMH
                     LEKGYDTMLENDGANLSQGQRQLLNIARASISKAPILILDEATSSVDTRTERHIEKGM
                     DALMSDRTTFVIAHRLSTVRNANAIMVLEKGEIIERGDHDDLLQQRGRYYELYSELAE
                     LD"
     gene            1400..>2420
                     /locus_tag="PKV59_07520"
     CDS             1400..>2420
                     /locus_tag="PKV59_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQP46635.1"
                     /translation="MESNASQNGQGATSEPFDNMETLLESKGLNIDFPQQGEIRQGVI
                     ASITPDQILVNVGTKSEGIISGKELEAISKSERDSLNVDQKIAVYILDPEDSNGNLVL
                     SYTRAREEESWKRAEQLLNSKEPFETKIVGYNKGGLIVELGSLRGFIPASQISLSRRV
                     DVSGETPEQRWGKMVNQPIKVSVIEVDRERRRLICSERAASTETRESLKEHVIESLKE
                     GDIRTGRVASLADFGAFINISGADGLVHLSEISWDHIKHPSEVLQVGQEVKVKVISID
                     KEKKRIGLSIRQTQEDPWAERVAKYQVGQLVEGTITRLTKFGAFAKLDDNIEGLIHIS
                     EISEKR"
BASE COUNT          731 a          519 c          582 g          588 t
ORIGIN      
        1 gccggaaacc gtacagtcaa cagcagaccg cattgggaat cttggatggt ttcgcacaag
       61 aaacgatcaa cggccaaaag gtggttaagg tcttcacccg tgaagagatc gtcaaggatg
      121 aattcaaata tttaaatgat gatttacgcc gaaaacagat cgtagctcaa tttcgttcct
      181 caatcatggg accggttact cataacttga gcaactttag ctacgctttg acgaccggca
      241 tcggatccat tttatgtatt acgcaaggat ttgatatcgg aggtttgacg gtttttgtga
      301 actacactcg tcagttcagc cggccgatca atgaaatctc gatgcaaatg aacaccgtct
      361 tcgccgcatt ggcgggggcg gaaagggtat tcgagttgtt ggatgagccg ccggaaccgc
      421 cggatgcacc ggattgtgtc aacgttgatc atctggccgg acatgttgtg atgagtcacg
      481 tcaatttcgg gtattacccg gacaaacttg tccttcacga tatttctctt tatgcaaaac
      541 ccggacaaaa aatcgctttt gtcggttcca ccggtgccgg aaagaccaca ataaccaacc
      601 ttcttactcg tttttatgat atccaatccg gagaaatcac gatcgacggg atcccgatcg
      661 ataaaatcca gcgtaagata ttacgtcgaa acattgcgat ggttcttcag gatacccacc
      721 tgtttaccgg aaccgtgcgc gagaatatcc gatatggtcg attggacgca accgacgacg
      781 atgtaatcgc cgccgcaaaa gtcgccaatg cccattcctt tatcatgcat ctcgagaaag
      841 gatatgatac gatgctcgaa aatgacggag ctaaccttag ccaaggacaa agacaattac
      901 tgaacattgc cagagcttcc atctccaaag cgccgatcct gatactggac gaagcaacca
      961 gctcagtcga caccagaacg gaacgccata tcgaaaaggg aatggatgca ttaatgtccg
     1021 atcgcacaac cttcgtgatt gctcaccggc tttccacagt ccgaaatgcc aatgccatca
     1081 tggttctgga aaagggagaa atcatcgaac gaggagatca cgatgatctg ctacaacaaa
     1141 ggggacgata ttacgaactt tattccgaat tggcggaatt ggattaatgg attttttcaa
     1201 tgctgttatg ccgaaacgca gactttcggc atagaaaaat cggttcggga ataatttcgt
     1261 ttataatatg tgttacaaaa ttcgtgatac cgataaaaaa acggaatatt taacgggata
     1321 tgagtcattt atcttgtcaa caataaaaaa gactcgtata atgattaaat aaattgtttt
     1381 ttggagaaat tacaaagtga tggaatcaaa cgcatcccaa aacgggcaag gggcaacttc
     1441 tgaacccttt gacaacatgg aaacccttct cgagtcgaag ggacttaata tcgattttcc
     1501 ccaacagggt gaaattcgtc agggagttat cgcaagcatt acaccggacc agattctggt
     1561 aaatgtcgga acaaagtccg aagggattat ttccggaaaa gaactggaag caatttcaaa
     1621 atcggaacgc gatagcttga atgtggatca aaaaatcgcc gtttacattc ttgatcctga
     1681 agattccaac gggaatctgg tcctttccta cacacgagca cgggaagaag aaagttggaa
     1741 gcgagcggaa caattgctta attccaaaga accgttcgag acaaaaattg tcggctataa
     1801 caaaggcggc ttaattgtcg aattgggttc acttcgcggt tttattcctg cttctcaaat
     1861 cagtttgtcc agacgggtcg atgtcagcgg ggaaacaccc gaacaacgat gggggaagat
     1921 ggtcaatcaa ccgattaaag tttccgtgat tgaagtggat cgcgaacgaa ggcgtttgat
     1981 ttgctccgag cgggcagcca gtaccgagac ccgcgaatct ctgaaagaac atgttatcga
     2041 atctttgaaa gaaggggata tccggaccgg tcgggttgcc agtctggcgg atttcggcgc
     2101 atttattaat atcagcggag cggacggact tgttcacctt tccgaaatat cgtgggatca
     2161 tattaaacat cccagtgagg ttttgcaggt cggacaggaa gtaaaggtaa aggtcatcag
     2221 tatcgataag gaaaagaaac gaatcggttt gtctattcgt caaactcagg aagatccgtg
     2281 ggccgaacgg gtggcaaaat atcaggtcgg gcagctggtc gaagggacaa tcactcgatt
     2341 gacaaaattc ggtgcctttg caaaactgga tgacaatatc gaaggtctaa tccatatttc
     2401 ggaaatcagt gaaaaacgga
//
LOCUS       DAOMIV010000325         2412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_48645_length_2412_cov_4.080611, whole genome shotgun sequence.
ACCESSION   DAOMIV010000325 DAOMIV010000000
VERSION     DAOMIV010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48645_length_2412_cov_4.080611"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..580
                     /gene="dcd"
                     /locus_tag="PKV59_07525"
     CDS             8..580
                     /gene="dcd"
                     /locus_tag="PKV59_07525"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQP46636.1"
                     /translation="MGVLCDHAIKELIDNGLIDFNRSEITYESVITQVNPNTLDLSLG
                     RFVRWPKEHSGQVIFGKAHHSDDFWELQEIDPKEGILLKPGDIFLGSSREFLRMPGDI
                     CGQVYTKSSLGRLFINHMMAGVIDAGFQGTITLELKNEGKHNVIIPYGARVVQIQFSR
                     LESKPDRDYSQRQNRYQGQIMPETAREEKN"
     gene            complement(577..1536)
                     /locus_tag="PKV59_07530"
     CDS             complement(577..1536)
                     /locus_tag="PKV59_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQP46637.1"
                     /translation="MFGRGSVQPILFIQSIKEAALHKIDLHMHSKYSNDGEFEPHTLM
                     DLCSKVPLDLVSLTDHNSAKGNVEAAKRAKELNMLFIPGIEINCQTKKRTEIHMLGYG
                     IDPTDSAFEKIEKEVIEREYEADLLRIRIFRKLGVILDEEKIQKETEKQPLTYRVIAE
                     VALSEYRNRDHPLLQPYLPGGNRSDNPLVNFFWDLCTTGKPADVPVDYLPVPKAIQLI
                     QESGGIAVFAHPGASIGKNDAELAEIVEAGISGIEVYSSYHDREMTRFYEIQAQKYGL
                     RVSVGSDFHGSLKPAIQLGSVSCNGREEEIYSFMQESISSSPA"
     gene            complement(1536..>2412)
                     /gene="thrS"
                     /locus_tag="PKV59_07535"
     CDS             complement(1536..>2412)
                     /gene="thrS"
                     /locus_tag="PKV59_07535"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQP46638.1"
                     /translation="RSYRDLPIRYAEMGTVYRYERSGVLHGLTRVRGFTQDDAHHFCR
                     PDQMPAEIDFVVEFSLHILRAFGFEEFQAYLSTKPEKSVGEPERWKAAESALEDSLKR
                     HNLPYEIDPGGGAFYGPKIDFKVKDAIGREWQLTTIQFDFNEPERFDLTYIAEDGQPT
                     RPYMIHRALLGSMERFFGVLIEHYGGAFPAWLAPVQAEIIPIADRHIDYANELAAQLR
                     QNRFRVHVDTRTDRMNAKIRDAQKEKIPYMLIVGDKEMENQQVSLRKRSGEDLGAMPV
                     ADFISLATDEVEKKI"
BASE COUNT          624 a          588 c          524 g          676 t
ORIGIN      
        1 aggataaatg ggcgttctct gtgaccatgc gatcaaagag ctgatcgata acggattgat
       61 cgattttaat cgttcagaaa taacttacga atcggttatt actcaggtca atcccaatac
      121 tttagatctt tcattgggac gttttgttcg ctggccgaaa gaacactcgg gacaggtaat
      181 tttcggaaaa gcccatcact cggatgattt ctgggaattg caggaaattg atccgaagga
      241 aggaattctg cttaaacccg gcgatatatt tctcggatcc tcccgagaat tccttcggat
      301 gcccggtgat atttgcggtc aggtctatac gaaatcaagt ttgggtagac tttttataaa
      361 tcatatgatg gccggcgtga ttgacgccgg tttccaaggt accattacat tggaactgaa
      421 aaatgaagga aaacacaatg tgattattcc ctacggcgcc cgggttgttc aaatccaatt
      481 cagccgtctg gagtccaaac ccgacagaga ttattcccaa cgtcagaatc gctatcaagg
      541 gcagattatg cccgaaaccg cgcgagagga aaaaaactaa gcaggcgatg aagagatcga
      601 ttcttgcata aaagaataga tctcttcctc tcttccgtta caggatacac ttcccaattg
      661 aatcgccggc ttcaagcttc catggaaatc acttccgaca ctgacccgca agccgtattt
      721 ctgcgcctga atctcataaa atcgagtcat ttcccgatca tgataactgc tgtatacttc
      781 aatcccgcta atgcccgctt ccacaatttc ggctaattcg gcgtcgtttt tcccgatact
      841 cgcaccggga tgagcaaata ccgctatacc gccgctttct tgaataagct ggatcgcctt
      901 cggaacgggt aaataatcga cgggtacatc ggcgggtttt cctgttgtac ataaatccca
      961 aaagaaattg accaagggat tatcgctgcg atttccgccc ggcagataag gctgcaacaa
     1021 gggatgatcg cgatttctgt actcgctcaa agcgacttcg gcgatcaccc gataggtcaa
     1081 tggttgtttc tcggtttctt tttgaatctt ctcttcgtct aagatgactc cgagtttacg
     1141 aaagattctg attcgaagta aatccgcttc atattctctt tcaatgacct ctttttcgat
     1201 tttttcgaaa gcactgtccg taggatcgat cccgtatcca agcatatgaa tttcggttcg
     1261 tttttttgtt tgacagttaa tctcaattcc gggaataaaa agcatattta attctttggc
     1321 tctttttgcc gcttcgacat ttccttttgc cgaattatga tcggtcagag aaaccaaatc
     1381 cagcggtacc tttgaacaaa gatccatcaa cgtatgcggc tcaaattctc cgtcgttact
     1441 gtattttgaa tgcatatgta aatcgatctt atgcaaagct gcctctttta tggattgaat
     1501 aaagaggatc ggctgtaccg atcctcttcc gaacatcaga ttttcttttc gacttcatca
     1561 gtcgccaatg agataaaatc ggcgacaggc atcgctccca agtcttctcc gctgcgtttt
     1621 cgtagggaca cctgttgatt ttccatttcc ttatcgccga cgatcagcat atacggtatt
     1681 ttttctttct gagcatcgcg aatttttgcg ttcattcgat ccgtccgggt gtccacatga
     1741 acccggaagc ggttttgccg cagctgcgcc gccaattcat tggcataatc gatatggcga
     1801 tcggcaatcg gaatgatttc ggcttgaacc ggagccagcc acgccgggaa agctccgccg
     1861 taatgttcaa tcagaacgcc gaaaaagcgt tccatgcttc ccaacagcgc ccgatgaatc
     1921 atgtaaggtc gagtcggttg accgtcttca gcgatatagg ttaaatcaaa tcgctccggc
     1981 tcattgaagt caaattggat agtagtcaac tgccattctc gtccgatggc atccttcacc
     2041 ttaaaatcga tctttggtcc gtaaaaagcc ccaccgcccg gatcgatttc ataaggtaga
     2101 ttatgtcgct tcagcgaatc ttccaaagcc gattcggctg ctttccaacg ttccggctct
     2161 cctactgatt tttcaggttt ggtactgaga tatgcctgaa actcttcaaa accgaacgcg
     2221 cgcagaatgt gcagggaaaa ttccacaaca aaatcgattt ccgcaggcat ttggtcggga
     2281 cggcaaaaat gatgagcatc gtcttgcgta aaaccgcgaa cgcgggtcag tccgtgcaga
     2341 acgccgcttc gttcataacg gtaaaccgtt cccatttcgg cgtatcggat cggtaaatcg
     2401 cgataagaac gt
//
LOCUS       DAOMIV010000326         2407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_48802_length_2407_cov_3.712160, whole genome shotgun sequence.
ACCESSION   DAOMIV010000326 DAOMIV010000000
VERSION     DAOMIV010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48802_length_2407_cov_3.712160"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..1235
                     /locus_tag="PKV59_07540"
     CDS             141..1235
                     /locus_tag="PKV59_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQP46639.1"
                     /translation="MIKQKPKNTILSIIAIAILLVILELIGRSKIDRYYIRILQYWSI
                     YIIYGASFQLLYGYTGLLSLGHAGLIAIGAYAVGIFTISPATKTMSYILQPPAPFISN
                     VQIPFVPALIIGGVFAALFGLVVAAPALRLGGDYLAIVTMGFAEVIRLLIVNMPSIFN
                     GAMGLRAIPQSATLLMTWSVAIVCLFVMKRFENSTYGRALLAINEDEIGAEALGINVF
                     SNKVKAFVLSSFFAGIGGGLMASVLGTIDPNTFKSTLTYTAVTICVLGGIRSATGVII
                     ASGIYSIMSEFLRVFESPIKIGNFYFSGMPGMRVLVFGIMLLFLMLFRREGLLGSKEF
                     SWDWFFSLFKKKPPKEQEKTVHADKMGGSL"
     gene            1232..2002
                     /locus_tag="PKV59_07545"
     CDS             1232..2002
                     /locus_tag="PKV59_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46640.1"
                     /translation="MTPILEAKNLTMRFGGLTSVSDFNLTMEKGELVGLIGPNGAGKS
                     TVFNMICGFYKPTIGEIIFDGKNITGMKPYRIANMGLIRVFQNGRLFKNMNVIENMMV
                     SKHMRFNSNSLKAILRTPSHNREEQRVFDESVALLERIGLKEMMYEPTGKLPFGIQRK
                     LEVARALCANPSLLLLDEPVAGLNVEETNDMMNFVDQLRNEFGITILLIEHTMRVVMS
                     LCPKIIVIDHGETIAVGIPDEIRNNKRVIEAYLGVDEQ"
     gene            1999..>2407
                     /locus_tag="PKV59_07550"
     CDS             1999..>2407
                     /locus_tag="PKV59_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP46641.1"
                     /translation="MIQIEELNVRYGGVHALKGISFEVPDNSIVTLLGANGAGKTSTL
                     RSICGLVKPQSGHIYYGEDKKDLTKMEAAAIVRAGISMIPEGRRVFTGLTVEENLQMG
                     AWNRNDKDGIQEDIDVIFHLFPVLKDRFHQKALT"
BASE COUNT          679 a          471 c          540 g          717 t
ORIGIN      
        1 tgcagaaatt atgctgatcg gatttttccc tcagtatacc aattttcggg atatattttc
       61 ctatgcgatc atgattattt tcttgctttt ctgccccggt ggaattttca atgttaagat
      121 ccgcgaagag aaggtataaa atgatcaaac agaagccgaa aaacactatt ttatccatca
      181 ttgcaattgc tatccttctt gtaatattag aactgatagg gcgcagcaaa attgatcgct
      241 attatattcg gatccttcaa tattggagta tctatattat ctacggtgct tcatttcaac
      301 ttctctatgg ttataccggc cttttatctt tgggacatgc ggggttaatc gccatcggtg
      361 cttatgcggt cggcattttt acaatcagcc cggcaacgaa gacaatgtcg tatatacttc
      421 aacccccggc tccttttatc agtaatgtac aaattccttt cgtaccggca ttaatcatcg
      481 gcggtgtttt cgcggcactt ttcggattgg tcgtagctgc cccggccctt cgtttgggag
      541 gagattacct tgcaatcgta acaatgggct ttgcggaagt tatacgcctt ttgattgtca
      601 atatgccgtc cattttcaac ggcgcgatgg gattgcgcgc tattccgcaa tccgctacat
      661 tattaatgac ttggtcggtt gcaattgtct gtctgtttgt tatgaaacgt tttgaaaaca
      721 gcacttatgg tcgggcttta ttggcaatta atgaagatga aatcggagcc gaagcattag
      781 gaattaatgt attcagcaat aaggtcaaag cttttgtctt atcatcattt ttcgcaggca
      841 tcggcggcgg cttgatggca agtgtattgg gtacgattga tcctaatacc tttaaatcca
      901 ctttaaccta tacagctgtc acaatatgtg tgctgggggg aattcgcagt gctaccggtg
      961 tcattatcgc cagcggaatt tattcgatta tgtccgaatt tcttcgcgtt tttgaatctc
     1021 cgataaaaat cggaaacttc tatttcagcg gaatgccggg catgcgtgtt ttggtttttg
     1081 gaatcatgct tctattctta atgcttttca gaagagaggg cttattaggt tccaaagaat
     1141 tttcttggga ttggtttttc tcactcttta aaaagaaacc tcccaaagag caagagaaaa
     1201 cagtccacgc cgacaaaatg ggaggatcgt tatgactccc attcttgaag caaagaattt
     1261 gaccatgcga ttcggcggac taacctctgt atccgatttc aatttgacga tggagaaagg
     1321 ggaactggtc ggattgatcg gtcctaacgg cgcgggaaaa agtaccgtat ttaatatgat
     1381 ctgcggattt tataagccga cgatcggaga aatcattttt gacgggaaga acataaccgg
     1441 catgaaacca taccgaatcg caaatatggg gctaattcgt gtttttcaaa acggcaggct
     1501 gttcaaaaat atgaacgtta ttgaaaacat gatggtcagt aagcatatgc gttttaactc
     1561 aaattccctg aaagccattc ttcgcactcc atcccacaac cgcgaagaac agagggtttt
     1621 tgatgaatct gttgccctgt tggaaagaat cggtctgaag gaaatgatgt atgaaccgac
     1681 cggaaaactt ccgttcggta ttcaacgaaa attggaagtc gctcgtgcat tatgtgcaaa
     1741 tccgagttta ctattactgg atgaaccggt agccggatta aatgtcgaag aaacaaacga
     1801 tatgatgaat ttcgttgatc aactgagaaa cgaattcggt ataaccattc ttttgatcga
     1861 acatacaatg agagttgtta tgtccctttg ccctaaaata attgttattg atcatggcga
     1921 gacgattgcg gttggcatcc ctgatgaaat tcgtaataat aaaagagtaa tcgaagcata
     1981 cctgggggtg gatgaacaat gatacaaatt gaagaattaa atgtacggta cggtggcgtt
     2041 catgctttaa agggaatctc ttttgaggtt ccggataatt caattgtgac ccttcttggt
     2101 gcaaatggcg ccggaaaaac atccacactc agatctattt gcggtttggt aaagccacaa
     2161 tccggccata tttattacgg agaagataag aaggatttaa caaaaatgga agcagctgct
     2221 attgtccgcg ccggaatctc gatgataccg gaaggaaggc gcgtttttac cgggttgaca
     2281 gtagaagaaa acctccaaat gggagcttgg aaccgaaatg acaaagacgg aatacaagag
     2341 gacatcgatg ttatctttca tttgttcccg gttttaaagg atcgctttca tcaaaaagca
     2401 ctcacgc
//
LOCUS       DAOMIV010000327         2391 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_49281_length_2391_cov_3.389983, whole genome shotgun sequence.
ACCESSION   DAOMIV010000327 DAOMIV010000000
VERSION     DAOMIV010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49281_length_2391_cov_3.389983"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1629)
                     /locus_tag="PKV59_07555"
     CDS             complement(145..1629)
                     /locus_tag="PKV59_07555"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQP46642.1"
                     /translation="MAETFLELKDIHKSFGGVQALKGVDLTIRCGEIHCLAGENGSGK
                     STLIKVISGAHEPTKGEIYIEGERILHLNPIIAIEHGIQVIYQDFAIFPNLTVAENIA
                     MNRAVSTGAKLMNWKGARELALEAMEQIGAKMDPDVLVENLSVANRQMVAICRTIIND
                     AKLLILDEPTAALTSKEVQKLYAILRRLRDKGIAVMIVNHKLDEVFEIAERLTILRNG
                     EKISTGMIDEYDRNRFVKELTGRNISESFYEPKVSEEEIFRVENISRKGAFEDVSFSL
                     KRGDVFGITGLLDSGRGQIGDALFGINPIQSGKIFLHGKQIEINSTEDAVRHKIAYVP
                     EDRLTQGLFLDRSIQDNTIAASISKYFIRGKLDEQAMYQATVEWIKRIGCVAPSPEAS
                     VRTLSGGNAQKIVIAKWLNTDPALLILNGPTVGVDIGAKYDIHQILRRLAGEGIGVIV
                     ISDDLPELIQNCNHILVMKNGRVSEIVEPSEVNETELATMMSSQ"
     gene            complement(1740..>2391)
                     /locus_tag="PKV59_07560"
     CDS             complement(1740..>2391)
                     /locus_tag="PKV59_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQP46643.1"
                     /translation="SHEAAGMENIDYDIEAFDNDAYGRHFMERRGELTGGEGEYVLEV
                     GSLTSASHNQWVDGAKALQEEKFPKMKQYGDKIETKDDQSISYEKIKEVLTANPNIAA
                     IQGSAMADVAGAALAVEELGLAGKVKIVGTSLVSVSGKYVREGTIDMISFWDPALAGK
                     AMIALAQKVLDGEKIESGLDLGIEGYSNLTLDGKVLTGQAWVDVTKDNVDDPAYNF"
BASE COUNT          628 a          617 c          457 g          689 t
ORIGIN      
        1 agccaagttc accacggtag aaatactgtt gaaggcatgg ttcactgatc cgatcaggat
       61 tgccaatccg gcaataatat aaacgacgat catttcattt gacgtaagga aatttctaat
      121 tttctgattc atcggttccc tcactcactg gctgctcatc atcgtggcaa gctcggtttc
      181 attcacttcc gacggctcga cgatttccga aacccgtccg tttttcataa ccaaaatatg
      241 gttgcaattt tgtatcaatt ctggaagatc atcggatatg actatcactc cgatcccttc
      301 ccccgccagg cgacggagaa tctgatgaat atcatatttg gcgccgatat ccacaccaac
      361 cgtcggccca tttaatatca acaacgccgg atcggtgttc aaccatttcg cgatcacaat
      421 cttttgcgca tttccgccgg ataacgttcg aacagaagcc tccggagagg gagccacgca
      481 tccgattcgt ttgatccatt cgacggtggc ttgatacatt gcctgttcat ccaactttcc
      541 gcgaataaaa tatttcgaga tggaagccgc aatcgtatta tcctgaatcg atcgatccaa
      601 aaacaatccc tgagtcaagc ggtcttccgg gacataagca atcttgtgac ggacggcatc
      661 ttcagttgaa ttaatttcga tctgttttcc gtgcaagaaa atttttccgc tttgaatcgg
      721 gttaatccca aataacgcat caccgatctg cccgcgaccg gaatccaaca agccggtaat
      781 accgaaaaca tctcctcgct ttagtgaaaa actcacatct tcgaaagcgc ctttccggct
      841 gatgttttcc acccggaata tttcttcttc agaaactttc ggttcataaa aagattcgga
      901 aatatttctc cccgtcaatt ccttgacaaa gcgattccga tcatattcat caatcatccc
      961 ggtggaaatt ttttcaccgt ttcgcaatat ggtcagccgt tcggcgattt caaatacttc
     1021 atccaatttg tggttgacga tcattaccgc aataccctta tcacgaagtc tgcggagaat
     1081 cgcatacagt ttttgaactt ctttagaggt caatgctgca gtcggctcat ccaaaatgag
     1141 caattttgca tcattgataa ttgttcgaca gatggccacc atttgccggt tggcgacgga
     1201 cagattttcc accaaaacat ccggatccat ttttgcaccg atctgctcca tggcttcgag
     1261 cgcaagttcc ctggcgccct tccaattcat caattttgca ccggtactaa ccgctcggtt
     1321 catggcgata ttttccgcga cagtcagatt cggaaaaatg gcaaaatcct gataaatcac
     1381 ctgaatgccg tgttcaatgg cgataatggg attgagatga agaattcttt ctccttcgat
     1441 ataaatctca ccttttgtcg gttcatgcgc tccggaaatt acttttatca gagtggactt
     1501 tcccgaaccg ttctcaccgg ccaggcaatg aatctcaccg catctgattg tcagatcaac
     1561 gcccttcaaa gcctgaacac cgccaaatga tttgtgaatg tccttcagct ccagaaacgt
     1621 ctctgccatg tgcagcctcc tgtctgtcat gaaatcaata ttcgttctta acaaataccc
     1681 cataaagggc tctttatggg gtatttaatt caatccgcta aaaagattcc ccgaattaat
     1741 tagaaattat atgccggatc gtcaacattg tctttggtta catcgaccca agcctgtccg
     1801 gtaagaactt ttccgtccag cgtaaggttg gaataacctt cgatccccaa atccaaaccg
     1861 gattcgatct tttcaccgtc caggactttt tgagctaagg caatcatagc tttccctgcc
     1921 aatgcaggat cccagaagga gatcatgtcg attgtacctt cgcgtacata tttcccggaa
     1981 acggaaacta atgaggtacc gacaatctta acctttccgg ctaaaccgag ttcttccacc
     2041 gcaagcgctg caccagccac atcggccatg gcagagcctt gaatggctgc tatattcgga
     2101 ttggcagtta aaacttcctt aattttttca taggaaatag attgatcatc tttggtttca
     2161 atcttatcac cgtattgctt cattttaggg aatttttctt cctgtaaggc tttggcaccg
     2221 tctacccatt gattatgtga tgcggaagtt aatgagccga cttccagaac atattcgcct
     2281 tcaccgccgg tcagttcacc tcttcgttcc atgaaatgac gcccataagc atcattgtca
     2341 aaagcttcaa tatcgtaatc gatattttcc ataccggctg cttcatgaga a
//
LOCUS       DAOMIV010000328         2390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_49321_length_2390_cov_2.877088, whole genome shotgun sequence.
ACCESSION   DAOMIV010000328 DAOMIV010000000
VERSION     DAOMIV010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49321_length_2390_cov_2.877088"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>2390
                     /locus_tag="PKV59_07565"
     CDS             89..>2390
                     /locus_tag="PKV59_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HQP46644.1"
                     /translation="MKELLVEALRAGKIDIGGLYVAPVSDYSFDEAVFRNFYYGKKWL
                     LENLGIDTPLAREEDSPGHTSQFPQICQGFGMRFARFSRGPIGVFRWQSPDGSVILTH
                     TSGYSWGYHLRLGRNFDEKWKIGFPLRLKHAEKNNYPSPNLLLSDGDDNALPNPDGPA
                     LVEAWNKQGKSPEFVSATITEYFNSIENEQFNTFSGDMPNQWAQIAVFEPDVFEHIRF
                     MRHTLPVAELALSLHPAPESNNRLETVWRTLLECLDHNWGGRFQGAYGLEGDLYKLER
                     AFDARKMLSEITREAVDRISVEIPDKPDTVKIIVFNTLSHIASDYVSLDFDTNKFPTF
                     KNSSLEVLDSSGTFYEIQLSFIPDNESRKTLSFNAEDIPAMGYKVFYITKSKKKVANR
                     KSITAGEKNIIENNFYRIEIGSRSAGIKSIFDKEFNQEIIPRKPGNLLELFGIQFHFN
                     EVWALGFRFDPAPDGFYDNPDNEGKVGESIVITGEIKTAGEFLQDNIQLTHTDGPVFS
                     SLSRTAPFMSGSRIDQEIILFHGTKRIGLKTNIRWQGTENYLLCMSLPFGPADGVMRM
                     DVPFGIHRFGDEVPGYWGKSGSADPRIGTIASLLPNAVKKWLRSGFLEEKNKKLAKLK
                     ESLTKRMMKESESASRGLYNWLDVSNNDWGATLLTQHSPFDFTFGSNAILFASIRSSA
                     FFNGEHYLRRGDNTYEYSLISYRGSLIEADVPRMGRTANSPLSAFIAAPETDGVDVLP
                     PSASILTCTEKNIITTAVKPGKVKGEL"
BASE COUNT          712 a          504 c          519 g          655 t
ORIGIN      
        1 attaaaatta gcttcattaa atgtattatt tcgtttttca atcgaagata tatattcggt
       61 ttatcttttt cttaatgaaa agcctgagat gaaggaactt ttggtcgaag cgttgcgtgc
      121 cggaaagatt gatatcggcg gtctttatgt agcacccgta tcagattatt cattcgacga
      181 agctgttttc aggaatttct attacggcaa aaaatggctt ttggaaaatt tgggaatcga
      241 taccccctta gcgcgtgagg aggattcacc cggccacact tcccaattcc ctcagatatg
      301 tcaaggcttc ggaatgcgtt ttgcacgctt ttcaagaggc cctatagggg ttttcaggtg
      361 gcaaagtccc gacggttcag taattcttac gcacacatcc ggttacagtt ggggatatca
      421 tcttagactc ggcagaaatt tcgatgagaa atggaaaatt ggattcccat tgcgactgaa
      481 gcatgcagag aaaaataact acccctcgcc caacctttta ttatcagacg gcgacgataa
      541 tgcgcttccc aatcctgacg gtccggcact tgtcgaagca tggaacaaac aaggaaaatc
      601 gccggaattt gtttctgcaa caataactga atattttaat tcgattgaga atgaacaatt
      661 caatacattt tccggtgaca tgcctaacca atgggcacag atagccgttt tcgagccgga
      721 tgttttcgag catatccgtt tcatgagaca tactcttcct gtcgccgagt tagcgctttc
      781 attgcatcca gcgcctgaga gtaataatcg attggagacg gtatggcgga cactcctcga
      841 atgtctcgac cataattggg gcggccgttt tcagggtgcc tatgggctcg aaggcgattt
      901 gtacaaactc gaacgggctt tcgatgcacg caagatgctt tcagaaataa ctcgagaagc
      961 ggttgatcgg atttcagtcg aaattccaga caaaccagat accgttaaaa taattgtttt
     1021 caatacactt tcacatatag caagtgacta tgtttcattg gacttcgata cgaataaatt
     1081 cccaacattc aagaattctt ctttagaagt attggattct tcaggaacat tctatgaaat
     1141 tcaattgtct ttcatccctg ataatgaatc gagaaaaacg ttatcattca atgcagaaga
     1201 tatccccgct atgggttaca aggtttttta tattacaaag agcaaaaaaa aggtcgcaaa
     1261 tcgaaaatct ataaccgctg gcgaaaaaaa cattatcgaa aacaatttct atcgaataga
     1321 aatcggaagc cggagcgcag gcatcaaaag catattcgat aaagaattta atcaagaaat
     1381 cattccaagg aaaccgggta acttattgga gcttttcgga attcaattcc acttcaatga
     1441 agtatgggcg ctcggtttca ggttcgatcc tgcgcctgat ggtttctatg ataatccgga
     1501 caacgaagga aaagtcggcg aaagcatagt tatcaccggt gaaatcaaaa ccgccggcga
     1561 attcctacaa gacaatattc aattaacaca tactgacggc ccggtgttca gctcgttatc
     1621 gcgaactgct ccttttatgt ccggaagcag aatcgatcaa gaaattattt tatttcacgg
     1681 tactaaacgg attggactta aaacaaatat tagatggcag ggaaccgaaa actatttgtt
     1741 atgcatgtct ctgcctttcg gtccggcaga tggtgttatg agaatggatg tgcctttcgg
     1801 tattcatcga ttcggcgacg aagtgccggg ttactgggga aagtccggtt ccgcagatcc
     1861 gaggataggt acaatcgcct ctttgcttcc caatgctgta aaaaagtggc tgcgatcggg
     1921 tttcctcgaa gaaaagaaca aaaagctggc aaaactcaag gaatctctca ccaagcgaat
     1981 gatgaaagaa agcgaatctg catcacgcgg actctataac tggttggatg tgtcaaataa
     2041 cgattggggc gcaacactac tcacgcagca ttcacctttt gattttactt tcggatcaaa
     2101 cgcaattctt tttgcctcca tcagaagcag cgctttcttt aacggcgaac attacctcag
     2161 gcgtggggat aatacgtatg agtattcatt aatttcatat agaggttctt taatcgaagc
     2221 agacgttcca agaatgggaa gaaccgctaa ttcgccgtta tctgctttta tcgccgcacc
     2281 tgaaacagat ggcgtagatg ttttgccgcc atcagcatcg attctgacat gcaccgagaa
     2341 gaatataatc acaaccgctg tcaaacccgg caaggttaaa ggtgaattga
//
LOCUS       DAOMIV010000329         2389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_49342_length_2389_cov_3.204799, whole genome shotgun sequence.
ACCESSION   DAOMIV010000329 DAOMIV010000000
VERSION     DAOMIV010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49342_length_2389_cov_3.204799"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /gene="secD"
                     /locus_tag="PKV59_07570"
     CDS             <1..1345
                     /gene="secD"
                     /locus_tag="PKV59_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQP46645.1"
                     /translation="DIVFQTAGENRILAEFPGMTNADEVLATLKGTGLLEFVDTGDQY
                     FPEGEAIKTDLQHSTSSETNAVNPTSSASSDTNVVNSSSSSSTESAGTAVTALQSSAT
                     ESITKSVETTAQPSAESSLGNAEGTTQPTAQTSENVEATPQPTAATTEQPAEEEKIYH
                     TVLTGSALKNVTVQMYNNQPVISFELKSEDAKTFADFTSQNVNKFLTIVLDGKVISSP
                     RINEPITDGKGVISGNFTIDSANALAIQLKYGALPVPLKVVEYKLIGATLGEDSLQKS
                     LVAGLIGLSIAVLFMLIYYRLPGLIAVLEIIFYGAVTLAIYKLIPVSLSLSGIAGFLL
                     TTGGAFDANILMFERLKEELRNGKTISQAVGLSWPRAWSSIRDSNIATLITAFILFYF
                     GSSFGASVVKGFAVSLIIGVLISMFSANFVTDTLLHVFTKSIADVPENKSKWFGI"
     gene            1358..2293
                     /gene="secF"
                     /locus_tag="PKV59_07575"
     CDS             1358..2293
                     /gene="secF"
                     /locus_tag="PKV59_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQP46646.1"
                     /translation="MKIDILSKRYWYFIFSLILIVPGLVLLAVKGMPYGIDFTGGTMM
                     ELQFSQGNRPEREAFENEIINYGIDATVVLSGENNVIIRAPYLEDSEIEKLIDYVAKQ
                     YSESPATLRYSISSVGPSIGSEVASRAALAVLAAALGVILYIWFAFRSIPNSFRYGVC
                     AVIAMIHDSLIVISLSCLGQFWGWQFDSLTLTALLTVIGFSVQDKIVVFDRIRENSRI
                     YRKLDFETLVNHSIVQTLERSINTQLMTSEFMLLAMALLGGSSLRDFSIILLVGLLMG
                     TYSSIFIAAPLFVVWQNKEWKNWFGHGNKKAVSEG"
BASE COUNT          625 a          532 c          538 g          694 t
ORIGIN      
        1 cgatatcgtc tttcaaacag ccggtgaaaa ccgaattctc gctgaattcc ccggtatgac
       61 caatgcggat gaagttctcg ctaccttaaa aggtaccggt ttgttggaat ttgtcgatac
      121 aggtgatcag tactttccgg aaggagaagc aatcaagaca gatttacaac actcgacttc
      181 gtcagagacg aatgctgtaa atcccaccag ttcggcttca tcagacacaa atgttgtaaa
      241 ttcttccagc tcgagttcaa cagaatcagc cggaaccgcg gttaccgctt tacaatcatc
      301 cgctacggaa tcgatcacga aaagtgtcga aactaccgct cagccatctg ccgaatcctc
      361 attgggtaat gcggaaggaa caactcagcc gactgcgcaa acctcagaga acgttgaggc
      421 tactccacaa ccgaccgctg caactaccga acaacctgcc gaagaagaga aaatttacca
      481 tactgttctg accggctccg ctttaaaaaa tgtaaccgtc cagatgtata acaaccaacc
      541 ggtaatctct ttcgagctga aaagcgaaga tgccaagacg tttgcggatt tcacctctca
      601 aaatgtcaat aaattcctga cgatcgtatt ggatgggaaa gttatttcca gtccgcgcat
      661 caatgaaccg attacagacg ggaaaggtgt aatttcaggg aactttacca ttgattccgc
      721 caatgcactt gccattcaat tgaaatacgg cgctctgccg gttcctttga aagtcgtcga
      781 atataaattg atcggtgcga cgctgggcga agacagcttg caaaaaagct tggtagccgg
      841 actgatcggc ttatcgattg ccgttttatt tatgctgatt tactatcggt taccgggttt
      901 gatcgcggta ttagaaatta tcttctacgg tgccgtgaca ttggcgattt ataaactgat
      961 ccccgtctct ctctccttat ccggaattgc cggtttcttg ctgacgaccg gcggtgcgtt
     1021 tgatgcgaat atccttatgt tcgaacgatt gaaggaagag ttgcgaaacg gaaaaaccat
     1081 ttcgcaagcg gtcggcttga gttggccgcg agcttggtca tcgattcgtg attcaaacat
     1141 tgcaacttta attacggctt ttatcctttt ctacttcgga tcttctttcg gagcttctgt
     1201 agtcaaaggg ttcgctgttt ctcttattat cggtgttctg atcagtatgt tctccgctaa
     1261 ttttgtgaca gatacgctct tgcacgtctt tacaaaatca atcgccgacg tacctgaaaa
     1321 caagtctaaa tggtttggga tctaaggagg gaaaatcatg aaaattgata ttctgtcaaa
     1381 gcggtattgg tattttatat tttctttaat tctaattgtt cccggacttg tccttttagc
     1441 cgtcaagggt atgccgtatg ggattgattt caccggcgga acaatgatgg aattacagtt
     1501 ttcgcagggg aaccggccgg aacgggaagc ttttgaaaac gaaattatca attacggaat
     1561 tgatgcgacc gtcgtcttat ccggcgaaaa taatgtgatt attcgagcac cttatcttga
     1621 agatagtgaa atcgaaaaac tcatcgatta tgtcgcgaaa caatacagtg aatccccggc
     1681 gacattgcgt tacagcatca gcagtgtcgg accttccatc ggcagtgagg tggcaagtcg
     1741 tgcagcgctg gctgttctgg ctgcggcatt gggcgttatc ctttatattt ggttcgcttt
     1801 tcgttcgatt ccgaacagtt ttcgttatgg cgtttgcgca gttatagcga tgattcacga
     1861 ctcattaatt gtgatcagcc tctcctgctt gggccaattt tggggttggc agttcgattc
     1921 cttaactctg acagctttat tgacggtgat cggtttctca gtccaagata aaatcgtggt
     1981 ctttgaccgg attcgagaaa attctcgcat ctatcgaaaa ttggattttg aaacgttggt
     2041 taaccattcc atcgttcaaa ccttggaacg ctccattaac acccaactga tgacctctga
     2101 atttatgctc cttgccatgg cgctcttggg aggttcttcg ctgcgagatt tctccatcat
     2161 cttattggtg ggcttgttaa tgggtaccta ctcttccatt ttcattgccg caccgctatt
     2221 cgtcgtttgg caaaacaaag aatggaaaaa ctggtttggg cacggcaata aaaaagctgt
     2281 cagcgaagga taattttttc cgatcgaaaa caataaaaaa agcctcgaca aaaaattgag
     2341 gcttttttta ttgtttgagt tatcaggttg cagccagaat cctttacgg
//
LOCUS       DAOMIV010000330         2385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_49479_length_2385_cov_4.421459, whole genome shotgun sequence.
ACCESSION   DAOMIV010000330 DAOMIV010000000
VERSION     DAOMIV010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49479_length_2385_cov_4.421459"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKV59_07580"
     CDS             <1..599
                     /locus_tag="PKV59_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HQP46647.1"
                     /translation="KSVRSMGRQARGVRGMRIAADDRLACVDVAKEDDYLLVVGERGI
                     GKRTPVRNIPRHGRATGGVMITNKRTLDVTGKVISAIVLHENDDVTFITASGHVVRLK
                     GDSIPVNGRTARGVRLIRLAEGDTVAAVTSNSPDDIPVETLPESTDLEPETEDIENAD
                     EETDEDETEEITEDSEADSANESDDESPDDAADTDPDA"
     gene            complement(757..2166)
                     /locus_tag="PKV59_07585"
     CDS             complement(757..2166)
                     /locus_tag="PKV59_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP46648.1"
                     /translation="MFIDRKTELELLEQCYRSDQAELFVLYGRRRVGKTELLHAFCAD
                     KPHIFFIATLSSDSEQLATFSQQVYGFTHSDVPAGFTFPSWEAALHALSEFPTQPKPI
                     IVLDEFTYLISGNKAIPSILQKVWDETLKNTPIMMVLCGSYIGMMEKEVLGYQAPLYG
                     RRTASFLLQPMDLPSSALFFPGYSAEEKFITWAVVGGMPFYLRTFRDKRDIFSNIRQH
                     ILDPQRGTLFNEPRLLLMEELREPRNYFSILSAIAQGRTRLNEIAQGAGIGDVTTVAR
                     YLDILQQMRLITRRVPATETQPQKSKKGIYHIDDHFLRFWFRYVHPNQSSLDLGLADA
                     ILRQRIKPDLDHFVANAFEEAAITFTGHLAQAGELGFIPERIGGWWNRDAEIDVLAIN
                     LTEKIALVGECKWNIHPVGSGVLEDLKQKAEILMKGHEIKQVRYAIFARNGFTADLVE
                     RSKNEGIYLFTVDTLVNLI"
BASE COUNT          641 a          530 c          584 g          630 t
ORIGIN      
        1 aaaaaagtgt ccgttcgatg ggcaggcaag ccaggggtgt gcggggtatg cgtatcgccg
       61 cggatgatcg tttggcatgt gtcgatgtgg caaaagagga cgattattta ttggttgtcg
      121 gagaaagagg gatcggcaaa cgtacgccgg tccgaaacat ccctcgccac ggtcgggcga
      181 ccggcggcgt gatgatcacc aataaacgca cattggatgt gaccggtaag gtgatttccg
      241 cgatcgttct gcatgagaat gacgacgtta ctttcatcac cgcttcgggg catgtcgtcc
      301 gtttgaaagg cgattcgatt ccggtaaacg gtcggaccgc acgcggagtt cgtctgattc
      361 ggttggccga aggggatact gttgccgcgg ttaccagcaa ttctccggat gatataccgg
      421 tcgaaaccct tcccgagagc accgatttgg agccggaaac ggaagatatc gaaaacgctg
      481 atgaagaaac agacgaagat gaaacggaag aaattacgga ggatagcgaa gccgattccg
      541 cgaatgaaag cgatgatgaa tcaccggatg atgcggcgga taccgatccg gatgcttgat
      601 tttcgtctgg cttctaaata aaatagactt gtttgaagaa atccccgaaa gcggggattt
      661 cttttttacg agagctttac tgtattttcg cctcaagttt tcttgagtat cgtaaaggaa
      721 ttcggctgat tctaagggga tatcaagaat ttcaggctaa atgaggttta caagggtatc
      781 aaccgtaaac aggtagatcc cctcattctt ggatctttcc acgagatcag cggtaaaacc
      841 gttacgcgca aaaatggcgt atcgaacttg tttaatttca tgacctttca ttagaatttc
      901 agctttttgc ttgagatctt ccaaaacccc gctgccaacc ggatgaatat tccatttaca
      961 ctcacccacg agagcaatct tctcggtaag gttaatcgca agaacatcaa tctctgcatc
     1021 ccgattccac cagcccccaa ttcgttcggg gataaagccc aattcgcctg cttgtgccaa
     1081 atgtcccgtg aacgtaatcg ccgcttcctc aaaagcattg gcaacaaaat gatctaagtc
     1141 cggtttgatc cgctgtcgga gaatcgcatc tgccaatccc agatcaagac tgctttgatt
     1201 gggatgaaca tagcgaaacc agaaacgcag gaaatggtcg tcaatatgat aaattccctt
     1261 tttgctcttt tgcggttgtg tttcagtagc cggtacacgc cgggtaatta gcctcatttg
     1321 ctgtaaaata tccagatagc gagccacggt ggttacatcc ccgatgcctg cgccttgtgc
     1381 gatctcgttc aaacgtgttc ggccttgcgc gatcgcactc aaaatggaaa aataattgcg
     1441 cggctctctt aattcttcca tgagaagtaa acgcggttca ttgaacagcg tgcctctttg
     1501 cggatctaaa atatgctgcc ggatattgga aaaaatatcc cgcttatccc ggaacgtgcg
     1561 cagataaaac ggcatgccgc ctacgactgc ccaggtgatg aatttttctt cggcagaata
     1621 gcccggaaaa aacagcgcag aggaaggcag gtccataggc tggaggagaa agcttgctgt
     1681 tctccgtcca tacagaggag cctgatagcc gagtacttct ttttccatca tgccgatgta
     1741 tgaaccacat aataccatca tgattggtgt gtttttaagc gtttcgtccc atactttttg
     1801 cagaatggat ggaatggctt tgtttccgct gatcaaataa gtgaattcat ccagaacgat
     1861 aatcggcttg ggttgcgtgg ggaattcgct caaagcatgc aatgccgctt cccaggaagg
     1921 gaatgtgaat cctgcgggaa catccgagtg ggtgaaacca tatacctgct gagagaatgt
     1981 ggctaattgc tctgaatcac tgcttaatgt ggcgataaaa aagatgtgcg gtttgtctgc
     2041 gcaaaacgca tgcagcagtt ccgtttttcc gacacgacgg cgaccgtaca aaacgaaaag
     2101 ctctgcctga tcggatcgat aacattgttc caataattca agttcggtct tgcggtcaat
     2161 aaacaaagtc ccctccgtga aaaaatatct aaatacgaaa cacaattatt ataatcgtga
     2221 ttcgaattta ttgcaatctg tgtattctat cccttttttc cgctttggag gaattctttt
     2281 ttacggaagt tttactgtaa tttcgcaatt aatttacggg gcagtcgggg attgattcta
     2341 taattcgttg tgatgccgtt acagaaaagg aattgaaatt atgat
//
LOCUS       DAOMIV010000331         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_50076_length_2365_cov_5.951515, whole genome shotgun sequence.
ACCESSION   DAOMIV010000331 DAOMIV010000000
VERSION     DAOMIV010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50076_length_2365_cov_5.951515"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PKV59_07590"
     CDS             complement(<1..752)
                     /locus_tag="PKV59_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46649.1"
                     /translation="MNTQRMERLAKKYGNTNHNGVARPKTFAINTMSALRAKSLSSND
                     KPKNMGYTIKRLLDYLENDKKLLITAIACILFQTIATLAGSYMIRPIINTYITPKNGQ
                     GDLHGLANSLVILAFIYLSGVILQYLQLRIMVGVSQRSLVRLRRDLYNKLLNLPIRFF
                     DTNSSGDIMSRFTNDVDAVGEMLNTTIVQLVAGTITLIGTLGLMIYTNIWLTLITLVM
                     IPIFTYASRSIIQFSRKPYSQQQTALGILDGFA"
     gene            complement(752..>2365)
                     /locus_tag="PKV59_07595"
     CDS             complement(752..>2365)
                     /locus_tag="PKV59_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46650.1"
                     /translation="NGAVNHDIPYILRTGGLMVGFSVIMAVFGTLGAKFAVNGSVNLA
                     ADLRFDVFSRIQEFSFKNIDDFSTGSLITRITNDITQIQTFAMSLLRGVFRSPVMLIG
                     AVIMAFRLNSQLAIVILIVIPVLGSAIFTIITKASPRFTTMQEQLDKLNSDISESLTN
                     IRVIKSFVREDYENKDFSVVNEGLREKSIAAMEMVILQQPVITATMNITTLAVVWFAG
                     RQIIVGSMPIGNLAAFITYITQILMSLNLLANIFLQGSRAIASSKRINQVMETTIELD
                     DSHAAFPEAMVEKGEIEFRHVSFRYFKTNHENVLDDINLAISAGETVGMIGSTGSGKT
                     TMVQLIPRLYDADEGEVLVDGINVKDYSLKKLREGVALVLQKNTLFSGTIAENLQWGN
                     EYATEEEMRAAARIAQAESFIDSLPDGFETDLGQGGVNLSGGQKQRLCIARALLKKPK
                     ILILDDSTSAVDTATEAKIRKAFSENLQGITKIIIAQRINSVIDADKIVVLNEGKIVG
                     CGVHQDLIKTCVPYQEIYYSQKEKNKEGE"
BASE COUNT          644 a          539 c          510 g          672 t
ORIGIN      
        1 gcgaaaccat ccaagattcc caatgcggtc tgctgttgac tgtacggttt ccggctgaat
       61 tgaataatgg acctgcttgc ataggtaaaa atcggaatca taaccaaggt aatgagagtc
      121 agccaaatat ttgtgtagat catcaatccc aaagtaccga taagtgtgat ggttcccgca
      181 accagttgga cgattgtcgt attgagcatt tcaccgacag cgtcgacatc attggtgaat
      241 cgtgacatga tatctccgct ggagttggta tcaaaaaatc gaatcggcag gtttaaaagc
      301 ttattgtata aatctcgcct taatcgaacc aagctgcgtt gactgacgcc caccataatc
      361 cgcagttgta aatactgtaa aataacgccg gacaaataaa tgaaagccag aatgactaag
      421 gaatttgcca gcccatgaag atccccctgt ccgttcttcg gggttatgta ggtattgatg
      481 atcggacgga tcatatacga acccgcaagg gtggctattg tctgaaataa aatgcaagca
      541 atcgcagtaa tcagcaattt cttatcattt tccagataat caagtaaccg cttgatggta
      601 tagcccatat ttttcggctt atcatttgaa gataaggatt tcgcacgaag tgcagacatc
      661 gtgtttatcg caaaggtctt gggtcgagca acaccattgt ggttggtgtt cccatatttc
      721 tttgccagtc tttccattcg ctgtgtattc attattcgcc ctctttgttt ttctcttttt
      781 gcgaataata aatttcttga tacggaacac aggttttaat cagatcctgg tgaacaccgc
      841 aaccgacaat tttcccctcg ttgagcacga caattttgtc cgcgtcgatc acggaattga
      901 tccgttgggc aataataatt ttggtaattc cttgaagatt ctcggaaaac gctttgcgga
      961 ttttagcttc ggttgcggta tccactgcgc tggtactgtc atcaaggatc agtatttttg
     1021 gtttcttcaa taaagcgcgg gcaatacata aacgttgctt ctgtccgccc gaaagattga
     1081 ctcctccttg tcccaaatcg gtttcaaacc cgtcgggaag cgaatcgatg aaagattcgg
     1141 cttgtgcgat tcttgcggcc gctctcatct cttcttccgt ggcatattca ttcccccatt
     1201 gtaaattttc ggcaatcgtg ccggaaaaca gcgtattctt ttgaaggacc aacgcgacgc
     1261 cctcccgtag ttttttcagt gaataatctt tgacgttgat cccatccacc agaacttcac
     1321 cttcatcggc atcataaagg cgcggaatca actgaaccat cgtggttttt ccgcttccgg
     1381 tagagccgat catcccgacg gtttccccgg ctgaaatagc caggttgata tcatccaaaa
     1441 cattttcgtg attggttttg aaataacgga aggagacatg tctgaattca atttcccctt
     1501 tttccaccat cgcttccggg aaggcagcat ggctgtcatc cagttcaatc gttgtttcca
     1561 tcacctgatt gattcttttt gaagaagcga ttgcccgcga cccttgcaaa aagatattgg
     1621 caagtaagtt cagtgacatc aggatttgag taatataggt gatgaacgct gccagattcc
     1681 cgatcggcat actgcccacg atgatctgtc gtccggcaaa ccaaacaacc gctaaggttg
     1741 tgatattcat agttgcggta atgaccggct gttggaggat gaccatttcc attgcggcaa
     1801 tacttttttc acgtaaacct tcgttgacaa cggaaaaatc tttattttcg taatcttccc
     1861 gaacgaagga cttgataact ctaatgttcg tcagcgattc gctgatatcc gaattaagct
     1921 tatctaattg ctcttgcatc gttgtgaatc ggggggaggc tttggtgata atcgtgaaaa
     1981 ttgccgatcc gagaaccggt ataacgatta atatgacgat cgccaattgt gaattaagac
     2041 gaaaagccat tatcaccgca ccgatcaaca ttaccggaga acggaaaacc cctctcaata
     2101 aggacattgc gaaagtctga atctgggtaa tatcattggt gatgcgggta atcaaagatc
     2161 ccgtactgaa atcatcgatg tttttgaacg aaaattcctg aattctgctg aatacgtcaa
     2221 agcgtaaatc ggcagccagg ttaaccgatc cgttgacggc aaattttgca cccaaagtgc
     2281 caaaaacggc cattataacg gaaaagccaa ccatcaatcc gccggttcgc agaatgtaag
     2341 ggatatcatg gttaaccgca ccgtt
//
LOCUS       DAOMIV010000332         2353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_50467_length_2353_cov_4.383377, whole genome shotgun sequence.
ACCESSION   DAOMIV010000332 DAOMIV010000000
VERSION     DAOMIV010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50467_length_2353_cov_4.383377"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PKV59_07600"
     CDS             complement(<1..637)
                     /locus_tag="PKV59_07600"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQP46651.1"
                     /translation="MDIDKLPKYYLLKKELIDRIDREEFKQDEPIPSERELMSQFDVS
                     RITVRRAINELVNEGYLYTIQGKGTYIKNGRYSQNLLSLTSCTQDIIKMGMKPSRRIV
                     ASGIEMAEKKRQHLLHLPEGEKVYFLERVYFADDEPINLTKTWLPYRLVRGIEKFDFS
                     EASLYEVLQNDYHIELTSADRSIEAVLSDEETARLLGITPDIPILLFTCTTY"
     gene            complement(955..1026)
                     /locus_tag="PKV59_07605"
     tRNA            complement(955..1026)
                     /locus_tag="PKV59_07605"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(992..994),aa:Ile,seq:cat)
     gene            1157..2326
                     /gene="nagA"
                     /locus_tag="PKV59_07610"
     CDS             1157..2326
                     /gene="nagA"
                     /locus_tag="PKV59_07610"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HQP46652.1"
                     /translation="MKKILIKNAVIYTPTANGFNGWVIIEDKKILSIQRGSPIPETEQ
                     AMDQVIDAEGKALLPGFIDVHTHGGLGFCTMDADQERLKAIARRNAEHGVTSFLPTTL
                     TDTKEATIRAIEAVTARINVRDGGAKTAGIHLEGPYFNPVKAGAQDPDNIRRADKNEI
                     RDFLKAGKIRLIAMAPEYPENLAAGDLFAAEGITVSAGHTNASYDDLVKAAEHGFSEI
                     THLYNGMGAFGHREPGTIGGALTIPAYTCELICDNVHSHPATHRLAWKAKGPEKIILI
                     TDCIRPSGLPDGTYPFTDKEMVTVSEHGTNLRLKNGSLAGSALTMDRALFNFMNNTGT
                     TLDEIWRCGSLNAAIQIGISAETGSIEKGKLADLILLDAACQVKMTMIEGEIVYQ"
BASE COUNT          663 a          529 c          537 g          624 t
ORIGIN      
        1 cgtatgtagt acaggtaaac aatagaatcg gaatatccgg cgtaattccc aacaaccgtg
       61 ccgtttcctc atccgaaaga accgcttcga tactccgatc ggcgcttgtc agttcaatgt
      121 gataatcatt ttgtaaaact tcatacagtg atgcctcaga aaaatcgaat ttttctatcc
      181 cgcgaaccaa acgatacggc agccaagttt tggtcaggtt gatcggttca tcatccgcaa
      241 aataaaccct ttcaagaaaa tagaccttct caccttccgg aagatgcaga agatgttggc
      301 gttttttctc ggccatctcg ataccgctgg cgacaattcg ccggctgggc ttcattccca
      361 tcttgataat gtcctgagta cagctggtca gcgagagcaa attttgagaa tatcgaccgt
      421 tcttgatata ggtccctttc ccttggatgg tataaaggta gccttcgttt accaactcat
      481 tgattgcccg gcgaacggta atccggctca catcgaattg tgacatcaat tcgcgctcgc
      541 ttgggatcgg ttcgtcttgt ttaaattctt cccgatcgat acggtcgatc agttcttttt
      601 tgagcagata gtattttgga agtttatcga tatccatgaa aacctttcac tcgttatctc
      661 attatgttat tatgttgtta taaccagtta agatgattat atcgaatccg agaaagcctg
      721 tcagtgaaaa acatcactag ttttttatga caatttttca gattcctttt aaaaatatat
      781 caacagaggc gagatgattt cttcaagatc agattcgtac ggcagccatc cttttacgca
      841 aatctaaacc gactgggtag cgtatttaat aaccgataca gccgatcatc ggcatcatct
      901 aaccgagtta tcaattttgt aatctgtgaa attatttatc aaattaggaa tcagtgggcc
      961 cagtaggatt cgaacctacg accaagaggt tatgagcccc ctgctctgcc gctgagctat
     1021 gggccctgca actttccata ttataataga agttcgatca aattggaata atcacctgtc
     1081 atttcgtgga gattatcgtt ccaaaacaga tgaatcatag gaataaggaa accggcagaa
     1141 attgcgggag gtttttatga aaaaaatatt gatcaaaaat gctgtgattt atactccgac
     1201 cgcgaacggg tttaacggtt gggtaattat cgaagacaag aaaattttaa gcattcaaag
     1261 aggcagtccc attccggaaa ccgaacaagc catggatcaa gtgatcgatg cggaagggaa
     1321 agcattgttg cccggcttta tcgatgtgca tacccacggc ggtttaggtt tctgtacgat
     1381 ggatgccgat caggaacgac tgaaagcgat cgcacgtcgc aatgcggagc acggcgtgac
     1441 cagcttcctg ccgaccaccc tgaccgatac gaaagaggcc acaatccgtg caatcgaagc
     1501 tgtcaccgct cgaatcaatg tccgtgacgg tggggcgaaa actgcgggaa tccatctgga
     1561 aggaccgtat tttaaccccg taaaagcggg tgcgcaagat ccggataata ttcggcgtgc
     1621 ggataaaaat gaaattcgtg acttccttaa agcgggcaaa atccgattga ttgccatggc
     1681 accggaatac cccgaaaacc ttgcggcggg tgatttgttt gccgcggaag gcatcaccgt
     1741 atccgccggt catacaaatg cttcttatga tgatctggtt aaagcggcgg agcatggatt
     1801 ttcggaaatc acacatttat acaacggtat gggtgcgttc ggacaccgtg aacccggaac
     1861 aatcggcgga gccctgacaa ttcccgcata tacctgtgaa ctgatttgtg acaatgtcca
     1921 ttcccatccc gcaacccacc gcctcgcttg gaaagcaaag ggcccggaaa aaattatttt
     1981 gattaccgac tgcattcgac cgtccggcct cccggacgga acttatcctt tcacggataa
     2041 agaaatggtg acggtttccg aacatgggac caatttacgg ctgaaaaacg gcagtctggc
     2101 aggcagcgct ctgacaatgg atcgtgccct tttcaatttt atgaataata ccggtaccac
     2161 gctggatgaa atctggcgtt gcggcagttt aaacgcagcc atccagatcg gtatttccgc
     2221 ggaaaccggc agtatcgaaa aaggaaaatt agcggatttg attttattgg atgctgcttg
     2281 tcaggtaaaa atgacaatga ttgaagggga aatcgtttat caataggcat tttatggaag
     2341 actcttttga aat
//
LOCUS       DAOMIV010000333         2348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_50636_length_2348_cov_4.738334, whole genome shotgun sequence.
ACCESSION   DAOMIV010000333 DAOMIV010000000
VERSION     DAOMIV010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50636_length_2348_cov_4.738334"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>2348
                     /locus_tag="PKV59_07615"
     CDS             21..>2348
                     /locus_tag="PKV59_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46653.1"
                     /translation="MSEMRYGTFNALEKLKKKLWAEYDHDRATVIVCVWDGPQAADAI
                     AVFTALEETARRKEADVRIELKEIDGRDLEDQGPIVMLMPSYEIISHVVPADAPDLID
                     HVIQDYRVLGDFFRNDPENESRQLFAKPENQTARRSAKGFRLSKNMSGSEITPTLGDT
                     ILGAYVLASKKTANLFGRKEGTKEKTKSSTRIALEQAFEKITAKYDSSKPNIIICAWE
                     GKCASEAMDLYYAFRQAQDKLGSNARIHIKKIDAGGFKDQGPLVMTLPTRALYVKVRK
                     YDAETIIRRTILRNEVLEGLLYREKVQEEPKTLTLTELAILAADNHSESDENLRNGIQ
                     SQLPAFLPVLEPRKLALIRSDALRMLGLNHLRGLDDGTESSLISASDFYYAEKTKAEA
                     STKAIGISEAEEEPEEAEFLTVSQLMELAEHPDSQAKKSVKMPATGSRIDRFRYEHLS
                     QLLHSAEIRFAPKLIVLNGNHAAPEEAEAFASALQDVIDEKGLKIKVKIAATTYYACP
                     GVSLLLEPCGILYTDFTSADAERIIQKTLLEGRVLTDLLFSDPLTGNRMLLEDQIPFN
                     RQQARILTEHFYDVAADSLADYFSAGGYKALSKVLKSSENQSLNEIRESNLRSHYGLG
                     TPLWQKMETCRQQSEDEKYVVAISHCEPEDADVDGKLLAGSPHQVLEGLIIGAFLVGA
                     EKGYIFIRSANEKAVSLTEQAIRDARIYGLLGKNILGTSFDFDVELIRSDEPAVGGEE
                     TAVLRFIEGRKAEPVNRPPEPEEKGLWNKPTLCDNV"
BASE COUNT          663 a          484 c          635 g          566 t
ORIGIN      
        1 gccggaggaa aatcaagaaa atgagcgaaa tgcgatatgg cacttttaac gctcttgaga
       61 aactgaaaaa gaaattgtgg gcagaatacg accatgatcg ggcaacggtc atcgtttgtg
      121 tttgggatgg accgcaggca gcggatgcga ttgccgtatt tacggctttg gaagaaacag
      181 cccgccgaaa agaagcggat gtgcggattg agctgaaaga aatcgacgga cgcgatttgg
      241 aagatcaggg accgatcgta atgttgatgc caagctatga aatcatctcc catgtcgttc
      301 cggcggatgc gccggacctg attgatcatg tgatccagga ttatcgggta ttgggagatt
      361 ttttccgaaa cgatccggaa aatgagtcaa ggcagctctt tgcgaagccg gaaaaccaaa
      421 cggcgaggcg atccgcaaaa gggtttcgat taagcaaaaa tatgtccggt tcggaaataa
      481 ctccgactct gggagatacg atactgggtg cctatgtgct ggcttcaaaa aaaaccgcaa
      541 atcttttcgg ccgcaaagaa ggcacgaaag aaaaaacgaa atccagcact cggatcgcat
      601 tggaacaggc gtttgaaaag attaccgcaa aatatgattc ttccaagccg aatatcatta
      661 tttgtgcttg ggagggtaaa tgtgcctcgg aagcgatgga tttgtattat gcgttcaggc
      721 aggcgcagga caaactggga tccaatgccc ggatccacat caaaaaaatc gatgccggcg
      781 gatttaaaga tcagggtcct ttggtgatga ctcttccgac acgggcattg tatgtgaaag
      841 tgcgaaaata tgatgccgag acgattatcc ggcggacgat tctgcgaaat gaagtcttgg
      901 aagggctgct ttatcgggag aaagttcagg aagaaccgaa aacgttaacc ttgacggaat
      961 tagccatatt ggcggctgat aatcattccg aatccgacga gaatctgcga aatgggattc
     1021 aatcccaatt accggctttc ttgcctgttt tagagccgcg aaagttggct ttgatccgtt
     1081 ccgatgcttt acggatgctg ggattaaatc atctgcgggg cttggatgat ggcactgaaa
     1141 gttccttaat ttccgcctcc gatttttatt atgccgaaaa gacaaaggcc gaagcttcca
     1201 cgaaagcgat agggatatcg gaggcagaag aagagccgga agaggccgaa tttctgactg
     1261 tctcacaatt aatggagctg gctgagcatc ccgactcgca agcgaaaaaa tcggtaaaaa
     1321 tgccggcgac cggtagccgt atcgatcgtt ttcgttatga acatttatcg caactgcttc
     1381 attcggcgga aattcgtttt gctccaaaac tcatcgtgtt gaacggcaat catgccgctc
     1441 cggaagaagc agaagctttt gcttcagctc ttcaggatgt catagatgaa aaagggctga
     1501 aaatcaaggt aaaaattgcc gctacgacct attatgcatg tcccggagtg tcattgttgt
     1561 tggaaccttg cggaatcctt tataccgact ttacttcggc tgacgcggaa agaattatcc
     1621 aaaaaacatt attggaaggc cgggtcttga cggatttgct gttcagcgat ccccttacag
     1681 ggaatcgaat gctcttggaa gatcagatcc cctttaatcg acagcaagcc agaattctta
     1741 cggaacattt ttatgacgtc gcagcggatt cgttggcgga ctatttttca gccggcggtt
     1801 ataaggcttt aagcaaagta ctgaagagct ccgaaaatca gtcgttgaat gaaatccgag
     1861 aatcgaacct gcgcagtcat tacggtttgg ggactccgct ttggcaaaaa atggaaacct
     1921 gccggcagca atccgaagat gaaaaatatg tggttgcgat cagccattgt gaaccggaag
     1981 atgcggatgt ggatggaaaa ttgcttgcgg gttcacctca tcaggtattg gaaggtttaa
     2041 tcatcggggc gtttttggtc ggagccgaaa aaggttatat ttttatccgt tccgccaacg
     2101 aaaaagctgt ttcattaacg gaacaagcta ttcgggatgc tcggatatac ggattgttgg
     2161 ggaaaaatat tctgggcact tccttcgatt tcgacgtcga gttgattcgg tcggatgaac
     2221 cggcggtcgg cggagaagag acggctgtcc tgaggtttat cgaaggtcgg aaagccgagc
     2281 cggtcaaccg gccgccagaa ccggaagaaa aggggttgtg gaataaaccg accttatgtg
     2341 ataacgtg
//
LOCUS       DAOMIV010000334         2347 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_50683_length_2347_cov_3.175829, whole genome shotgun sequence.
ACCESSION   DAOMIV010000334 DAOMIV010000000
VERSION     DAOMIV010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50683_length_2347_cov_3.175829"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..2320
                     /locus_tag="PKV59_07620"
     CDS             86..2320
                     /locus_tag="PKV59_07620"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQP46654.1"
                     /translation="MLLDDLLAGLPDNYTQADKDLVEKAYYFAEKAHEGQKRASGEPY
                     FTHCVAVAMILSELKVQSAAIAAGLLHDTVEDTNVTLEDIRANFGDEIAKLVDGVTKL
                     TNIPKTNDYEKNFSPQNKIDNDPISVTDEIELGIQDSAAMRDRKRQLKNETLRKTFIA
                     MVDDVRVIIIKLADRLHNMRTLSHVSKEKQKRIAQETLDIYAPLANRLGIWRIKWELE
                     DLAFRYVHPAEYAQIAENLATTHAIREKEIEEIIERLKTVMESVKIDCEITGRPKHIY
                     SIYTKMIDRNKPLEMLRDLRGVRLIVKDIPSCYAALGQIHTHWRPIPGEFDDYIAVPK
                     DNFYQSLHTAVYYDDGKPLEVQIRTPEMHQNAEYGIAAHWRYKEHTKADPAFEKRLDW
                     LRQLMDWRKDVEDAGEFVEGMKSDVFQDRVFVFTPRGDVIDLPIGSTPIDFAYSVHTE
                     VGHRCRGAKVNGKMVPLSYRLKTGDQVSIITTKQGGPSRDWLNPNLGLVNTQRARSKI
                     RAWFKWQDREQNLTQGKEIVKKDLQRLGLSDIDMNEIAKELDFKNTDDLYVAVGCGDL
                     SSMRVVNKINEGLKVSPLQILQPRPIPTDKTKSSGITVIGTGSIYTTFAKCCNPAPGD
                     DIVGYITKGRGATIHKADCPNIIRVKNRERLINVSWGTPEQRYPVTVTVRAYDRQGLI
                     NDITNLLVSEDVNLENINVKVAHNYANIQIVLDVLNIVQLSRVLTLIEGLPNVVEAYR
                     NKPG"
BASE COUNT          712 a          479 c          558 g          598 t
ORIGIN      
        1 tttataattg ttatgcgaag gaatttatta gaaaatttga cctatttcat tgtttgtaat
       61 tgttttcagg aaggtctaaa aaaatatgtt gcttgatgat ctacttgccg gtttgccgga
      121 taactatacg caagccgaca aagatttggt ggaaaaggct tattattttg ccgaaaaggc
      181 acacgaaggc caaaaaaggg cttcgggtga gccttatttt acccattgcg tcgctgttgc
      241 tatgattttg agcgaactga aagttcaaag cgccgcaatc gccgccggcc ttttacatga
      301 caccgttgaa gatacaaatg tgaccttaga agatatccgg gcaaattttg gtgatgaaat
      361 cgctaaattg gtcgatggtg ttaccaagtt gaccaatatt cccaaaacaa acgattacga
      421 aaaaaatttc tcgccgcaaa acaagatcga taacgatccg atttccgtta ccgatgaaat
      481 cgaattgggt atccaagatt cggcagcgat gcgagatcgc aaacgtcaac tgaaaaatga
      541 aacattacga aagacattta tcgcgatggt ggatgatgtg cgagtcatca tcatcaaact
      601 cgccgatcgt ctccataata tgcgaaccct cagccatgtc agtaaggaaa agcagaaacg
      661 aattgctcag gagacattgg atatttatgc tcccttagca aaccggctcg gaatttggcg
      721 gataaaatgg gagctggaag atcttgcctt tcgatatgtt catccggctg aatacgccca
      781 aattgccgag aatttagcga caactcacgc aattcgcgaa aaggaaatcg aagagataat
      841 cgagagactg aaaacggtta tggagagcgt aaaaattgac tgcgaaatta ccggcaggcc
      901 gaaacatatt tactcgatct atacgaaaat gatcgaccgg aataaaccgc tcgaaatgtt
      961 aagagattta cggggcgttc gcttgattgt aaaagatatt ccgtcgtgct atgccgcttt
     1021 aggacagatc cacacccatt ggcgaccgat tcccggtgaa ttcgacgatt acatcgctgt
     1081 cccgaaggat aatttttacc aatctttgca taccgccgtc tattatgatg acggcaaacc
     1141 gctcgaggta caaattcgta ccccggaaat gcatcaaaat gccgagtatg gtatcgctgc
     1201 ccattggcgc tataaagagc ataccaaagc tgatccggct tttgagaaac ggttggattg
     1261 gttacgccag ctgatggatt ggcgaaaaga tgtggaagat gccggtgaat ttgtggaagg
     1321 catgaaatcg gatgtctttc aggacagagt ctttgttttt actccgcgcg gagatgtgat
     1381 cgatcttccg atcggttcta cgccgatcga ttttgcatac agtgtccata cggaagtcgg
     1441 tcatcgttgc agaggagcca aagtcaacgg caaaatggtc ccgttgagct atcgtctgaa
     1501 aacaggagat caggtttcga ttatcacaac gaaacaaggc ggaccgagcc gtgattggct
     1561 gaatccgaat ctcgggttgg tgaataccca acgcgcccgc tcaaaaatcc gagcttggtt
     1621 taaatggcag gatcgcgaac aaaatcttac tcagggaaaa gaaatcgtaa aaaaggatct
     1681 tcagcggttg ggactcagcg atatcgatat gaatgaaatc gctaaagagt tggattttaa
     1741 aaacaccgac gatctttacg tggcagtcgg ttgcggtgat ttatcttcga tgcgggtcgt
     1801 aaataaaatt aacgaagggc taaaagtcag cccgttgcaa atcttacagc cgaggcccat
     1861 accgaccgat aagacaaaat cttcgggaat taccgtaatc ggaaccggaa gcatttacac
     1921 gacctttgcg aaatgctgta atccggctcc gggtgatgat atcgtcggct atatcacaaa
     1981 aggacgtggg gcaacgatcc ataaagcgga ttgcccgaat attattcgag tgaaaaaccg
     2041 agaacgatta atcaatgtct catggggaac gccggaacaa cgatatccgg ttacggtaac
     2101 ggttcgtgct tatgatcggc agggattaat caacgatatt accaatttac tggtcagcga
     2161 agatgtaaat cttgaaaata tcaatgttaa agtcgctcat aattatgcga atattcaaat
     2221 tgtgctggat gtccttaata tcgttcagct cagcagagtt ctgaccttga ttgaaggttt
     2281 accgaatgta gttgaggctt atcggaataa acccggttaa ctcggctcga ttaactttgt
     2341 aattcaa
//
LOCUS       DAOMIV010000335         2342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_50827_length_2342_cov_3.013555, whole genome shotgun sequence.
ACCESSION   DAOMIV010000335 DAOMIV010000000
VERSION     DAOMIV010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50827_length_2342_cov_3.013555"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /gene="recA"
                     /locus_tag="PKV59_07625"
     CDS             <1..857
                     /gene="recA"
                     /locus_tag="PKV59_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HQP46655.1"
                     /translation="GPESSGKTTLCLHIIAEAQKMGGTAAFIDMEHALDPKYAMRLGV
                     NINELYVSQPDTGEQALEICETLVRSGAVDVVVVDSVAALVPRSEIEGDMGDSSMGVQ
                     ARLMSQALRKLSGAINQTKTAVIFTNQLRLKIGVMFGNPETTTGGQALKYYSSVRLDI
                     RRINSIKSGAEVVGSRVRVRVTKNKVAPPFLTAEFDIMYNEGISRTGDIVDLGTQLEI
                     ITKRGSFYSYNDIRLGQGRENAKDFLKANPELANEIEEKIRNAALGSDIPILSFSSDS
                     EEDSSEEE"
     gene            1010..1489
                     /locus_tag="PKV59_07630"
     CDS             1010..1489
                     /locus_tag="PKV59_07630"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HQP46656.1"
                     /translation="MTGCLRSQSEGVLSSQFIPPTLVATSFKTPTTDPFQPTIDPRGP
                     CTNDLTFIDDLTVPDGTVFEPGAEIVKRWQVENSGTCEWTAKYSVRILSGASMGAEPK
                     QKLEPIAPGETGSIEIVFYAPEETGEYYSQWQAYDDRGKPFGQDFFIDIIVSSQTGY"
     gene            1521..>2342
                     /locus_tag="PKV59_07635"
     CDS             1521..>2342
                     /locus_tag="PKV59_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HQP46657.1"
                     /translation="MKKNRFILLFLCLFLSIPLKVLAQSDSASITNYVSNRMQYKLIS
                     EAQCASDLASFYGYSIEPLEIMDKIPLTDDPNTGYVGYYDDEGGLPPNSYGVYQQPLS
                     KALQTLGVPAYGYQNYSLDQLKTSVRQGNPVICWVVGNTAAGTPVEYTPSSGQSTTVA
                     LHMNSVTVTGFDQSGLLIWDNGESYTRSYEAFEASWGVLGNRALIIMPDKTASDKTDW
                     WGQPGDEFWDSEQTANEPTSLPEITIQKYPVTETEDYFSFDTEQDQNDQFGVIPEV"
BASE COUNT          640 a          505 c          601 g          596 t
ORIGIN      
        1 acggtccgga atcctccgga aaaaccaccc tatgtctgca tattattgcg gaagcgcaga
       61 aaatgggcgg aacagcagct tttattgata tggagcatgc gctggatccg aaatatgcga
      121 tgcggcttgg cgtcaatatc aacgagttat atgtatctca accggatacc ggcgaacagg
      181 ctcttgagat ctgtgaaaca ttggtacgat ccggcgcggt ggatgtggtg gttgtggatt
      241 cggtggctgc acttgttccc cgttccgaaa tcgaagggga tatgggagat tcctccatgg
      301 gggtgcaagc tcgactgatg tctcaggctc tgcgaaagct ttccggcgcg atcaatcaaa
      361 ccaaaaccgc tgtcattttt accaaccaat tacgattgaa aatcggtgtg atgttcggca
      421 atccggagac cactaccggc gggcaggcat tgaaatatta ttcttcggtg cgactggata
      481 ttcgtcggat caattcgatc aaatccggag cggaagtggt tggcagccgg gtcagagtac
      541 gggtgacaaa gaataaagtc gcgccgccgt tcttgacagc cgaattcgat atcatgtaca
      601 acgaaggaat ttctcgaacc ggcgatattg ttgatttggg aacacaactc gaaatcatta
      661 ccaaacgggg ttcgttctat tcttataatg atatccgatt gggtcagggg cgcgagaatg
      721 caaaagattt cctgaaagcc aatccggagt tggcaaatga gatcgaagaa aaaatccgga
      781 atgccgcatt ggggagcgat atcccgattt tatctttttc ttcagattcg gaagaggaca
      841 gctccgagga agaataaaaa agtttttaaa agtgccgctg ctttaataac ggcgcttttt
      901 ttatgttaaa atcgatggca taaatattgc accaaaaaag atgatgatca gacagcgtcg
      961 taaaagattc ttattcctct gttccctgat gtttctctca acggcaattg tgaccgggtg
     1021 cttgcgttcg caaagcgagg gtgtactcag cagtcagttt attccgccga cgctggttgc
     1081 cacctctttt aaaacgccga ccaccgaccc atttcaaccg acgattgatc cgcggggacc
     1141 ttgtaccaac gatctgactt ttatcgatga cctgaccgtt ccggatggga ctgtttttga
     1201 acccggcgcg gagattgtta agcgctggca ggttgaaaac tcgggtacat gcgaatggac
     1261 cgccaaatat tccgttcgaa ttttatccgg tgccagtatg ggcgccgaac cgaagcaaaa
     1321 gcttgaacca atcgctcccg gcgagacggg ttccatcgaa attgttttct atgcgccgga
     1381 agaaacggga gaatattaca gtcagtggca ggcttatgac gaccggggaa aaccgtttgg
     1441 tcaggacttc ttcattgata ttatcgtaag ttctcaaacg gggtattaat cgataaaaac
     1501 caaaggcaga caatatttta atgaaaaaaa accgatttat tctccttttt ctgtgtcttt
     1561 ttctcagcat tccgctgaaa gttcttgccc aaagcgattc cgcatcgatt acgaattatg
     1621 tcagtaaccg gatgcaatat aaattgatca gcgaagcgca atgtgcgagc gatttggcaa
     1681 gtttctacgg ttattcgatc gaacctttgg aaattatgga taaaatccca ttaacggatg
     1741 atccgaatac cggttatgtc gggtattacg atgatgaagg aggacttccg ccgaactcat
     1801 atggggttta tcagcagcct ttgagtaaag cgctgcagac gttgggagtg ccggcttatg
     1861 gttatcaaaa ttattcgttg gatcaactga aaacatccgt ccggcagggg aatccggtaa
     1921 tctgttgggt ggtcggaaat accgccgccg gaacgccggt cgaatataca ccttcaagcg
     1981 gacaatcaac aacggtggcc cttcatatga attcggtaac cgtaacggga tttgatcagt
     2041 cgggactttt aatctgggat aacggagaaa gttatacccg atcttatgaa gcgtttgaag
     2101 cgtcatgggg tgttttgggt aaccgggcac tgatcatcat gccggataaa acggcaagcg
     2161 acaaaaccga ttggtggggt caacccggcg atgaattttg ggattcggaa caaactgcga
     2221 atgaacctac ttctctgccg gaaataacga tccaaaaata tcccgtgacc gaaacggaag
     2281 attatttcag ctttgataca gagcaggatc agaacgatca gtttggagtg atcccggaag
     2341 tg
//
LOCUS       DAOMIV010000336         2338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_50954_length_2338_cov_4.081910, whole genome shotgun sequence.
ACCESSION   DAOMIV010000336 DAOMIV010000000
VERSION     DAOMIV010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50954_length_2338_cov_4.081910"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="PKV59_07640"
     CDS             complement(<1..919)
                     /locus_tag="PKV59_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQP46658.1"
                     /translation="MDFTVLVVDDEENFREGIKQYLSRDVNYEILDAASLSEAREILN
                     RQPVDIIMLDVQVGKEYGPDLLYDINRIRPTPKTILITAYGEVEMAVDAMKNGAFDFL
                     SKPVSFPLLEATLKRAEDVIRLQKEIESLMGAYKKDFSFVVGQNEKMKRIFKDAQLAA
                     KAQVSILIGGETGTGKEVLAKFIHQNGPRANKLLVPINCAAIQPTILESELFGYEAGA
                     FTNAEKRKPGLFEVADGGILFLDEISSMSMDMQSKLLRAIEEKKIRHVGGTKEIPVDV
                     QIVAASNRNISEMIEKGDFREDLYYRLRIV"
     gene            complement(924..>2338)
                     /locus_tag="PKV59_07645"
     CDS             complement(924..>2338)
                     /locus_tag="PKV59_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP46659.1"
                     /translation="FIPEPIFEQYDLYDRLQKNIMSKPIYPYIEENLSHFSSFEGDRL
                     SDVSDEFAGSPIHFKIFPVSNSEQRIGYFILRFQDSALYRLDFYQTVLEILSIYFQGL
                     FENMEMTKSLYQCEFQNSLNHQIIDNISEGIIITNNEYQIVYINQISSIMFGFAPSDV
                     VGHPLDDLLVTNEGVRKIVQTLSSSENNSDETPFIQYLHRRSGESFPCHLRISTLDFD
                     EKNSYTIFVLTDVSESEESRLKTEQLTQRAFLGDFASLLAHEIRNPINNMNIWIQNIK
                     SLTNEQSDIYQAVNRIEEDCSRVSVLVNNILAFSKPLKLNLEEIDLEQYINDIIERWK
                     LNFARANIKTFFSAPENLPKVLIDPRSMEQVFNNLIGNAIDAFDNKGGVISFKISLHD
                     SKTGRKQVQISVSDNGPGIPDEIIDHIFEPFVTSKKKGNGWGLAVSKRIITSHKGTIQ
                     VKSYPGGTVFEITLPISTGV"
BASE COUNT          690 a          490 c          413 g          745 t
ORIGIN      
        1 cgactatgcg caatctataa tataagtcct cccggaaatc acctttttca atcatctcgg
       61 aaatatttcg attcgaagcg gcaacaattt gcacatcaac gggtatctct ttggtgcctc
      121 ccacatgcct gattttcttt tcttcgatgg cacgcaacaa ttttgactgc atatccatgc
      181 tcattgagga gatttcatcc aaaaatagaa tgccgccatc agccacttca aataaaccgg
      241 gttttctctt ttcagcattt gtaaaagcac cggcttcata tccaaacaac tcagattcaa
      301 gaattgttgg ttgaatcgca gcacaattga tgggtaccag cagtttgttc gctctcgggc
      361 cgttctgatg aataaattta gccagaacct ctttaccggt acctgtttcg cctccgatca
      421 aaatagaaac ctgagctttc gccgcaagtt gagcatcttt aaatatccgt ttcatttttt
      481 cattctgtcc gacgacgaat gaaaaatctt ttttataggc acccattaaa ctttcaattt
      541 ctttttgaag ccgaataaca tcctccgcgc gttttaaagt tgcctccaac agcggaaagc
      601 ttaccggctt cgaaagaaaa tcaaaagcac cgtttttcat tgcgtcaaca gccatttcaa
      661 cttcaccgta tgcggttatt aaaatagttt tcggtgtcgg acgaattcga ttaatgtcat
      721 aaagaagatc aggcccgtat tccttgccga cctgaacatc caacatgatg atatcgaccg
      781 gttgacggtt gagaatttcg cgagcttcgc ttaaagatgc ggcatccagt atttcatagt
      841 taacatctct cgaaagatat tgtttaatcc cttctcgaaa attttcttca tcgtctacca
      901 ctaaaacagt aaaatccatc ctcttatact cccgtactga tcggtaaggt aatttcaaaa
      961 acggtaccgc cggggtatga ttttacttgt attgtcccct tatgtgaggt aataatacgt
     1021 ttgctgaccg caagtcccca accgtttcct tttttctttg aagtaacaaa aggttcaaaa
     1081 atatgatcaa ttatttcatc cggaattccc ggaccgttat cggaaactga tatttgaact
     1141 tgttttctcc ctgtctttga atcatgcaaa ctgattttga aagaaataac cccaccctta
     1201 ttatcgaatg catcgatcgc atttccgatc aggttgttga atacttgttc catagaacgc
     1261 gggtcgatca atactttcgg taaattttcg ggtgcgctga aaaaagtttt gatattcgct
     1321 cgcgcaaaat tcaatttcca gcgttcgatt atgtcattga tatactgctc caaatctatt
     1381 tcttccaaat tcagcttgag tggtttggaa aacgccagaa tattattaac cagcacggaa
     1441 actcttgaac aatcttcctc aattcgattt accgcttgat aaatatcact ttgttcattc
     1501 gttaaactct ttatattctg aatccagata ttcatattgt taatcggatt tcgaatttca
     1561 tgggctaata aagatgcaaa atcacctaaa aatgctcgtt gggtaagttg ttctgttttt
     1621 agacggctct cctcagattc tgatacatcg gttaagacaa aaattgtata gctgtttttt
     1681 tcatcaaaat caagcgttga aatccggaga tgacaaggga agctttctcc gcttcgacga
     1741 tgtaaatatt gtatgaaagg tgtttcatcc gagttatttt ccgatgaaga taatgtttgg
     1801 acgatctttc tgaccccttc atttgttacc agaagatcat ctaaagggtg tccgacaaca
     1861 tcggaaggag caaaaccgaa catgatcgat gatatttgat tgatatatac gatttgatat
     1921 tcattattgg taataatgat gccttctgaa atattatcga taatctgatg atttaatgaa
     1981 ttttggaatt cgcattgata caacgactta gtcatctcca tattttcgaa taaaccctga
     2041 aaatatatac tcaggatttc aagaaccgtc tgataaaaat ccagacgata taaagcgctg
     2101 tcctgaaatc ggagaatgaa atagccgatc ctttgttcgg aatttgacac gggaaatatt
     2161 ttaaaatgaa tgggactccc cgcaaattca tcggaaacat cacttaacct gtctccctca
     2221 aaagaagaaa aatgacttag attttcttca atatagggat agattggttt tgacatgata
     2281 ttcttctgca accggtcata aaggtcatat tgctcgaaaa tgggttcggg aataaatc
//
LOCUS       DAOMIV010000337         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_51079_length_2334_cov_5.516893, whole genome shotgun sequence.
ACCESSION   DAOMIV010000337 DAOMIV010000000
VERSION     DAOMIV010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51079_length_2334_cov_5.516893"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..>2334
                     /locus_tag="PKV59_07650"
     CDS             204..>2334
                     /locus_tag="PKV59_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQP46660.1"
                     /translation="MKASEIIRRRRRNRKNKVTRAVKSISALRILSFILICVAITFII
                     LCCAGYAVITQKLPPIENFDDHFASATEPTIIYDRTGTYPLLKLRYPTLEHVSLAIDE
                     PDKPCFSSDLVKIIVATHQPNFWSSVGADWHRWSNPDPVTIAERLVQQTFPNQLGSGV
                     EKAILTKILATQITKIYGPQAILTWYLNSAYFGQLAVGADAAARTYLDKSASDLNLSE
                     SILLSAILDTPSLNPVDAPGVMRESYLAQIEKLEENGTLTEEEIRSVRSTNFVIFEPP
                     PLEAEIAYSPGLQQALSSAYETIGQEAIERGGYKIRSTIDYDLQTLLECLTGIHHPSS
                     SDVTAQEKSCPYYPFETRTEDQTAEIAKILSQNPISAVVIDIKSGQMIASIDVGPDGS
                     GKRTARKVFSQHEPGSTLTPFVVLSALNRGISLSSMVWDLAEQRYYLPSGYQNPDGRL
                     QGPIRLRTAVDEDIMGPIAEMLREVGTAPVWNQATQFGITSVNARTPESILFNGGSVT
                     VDMLANAYLPFANQGRLIGTIYDDIPRPKTILSITSPEMAEIPAQMRSGISLLDPGLA
                     YLMVNLLAEDKQSYRYFNRPAGTKVGRVLSSNDFWMIGFTPEIMTVVWIGGTPNGAGT
                     DQAAVLLWQTIMAAAVKNQPITGWSRPETVSQLMVCNPSGKLPTDSCPDTVSEVFLAG
                     NEPTEYDDLYLRIPINRNNKKWRPPIQN"
BASE COUNT          627 a          552 c          544 g          611 t
ORIGIN      
        1 ttgcgatcaa gtggatgctg aactatatca acaaaaagcc gtttacgatt ttttcaattt
       61 actgctccgt catcggaatt tttactttta tcctcacatt catccgctga ttttttctgg
      121 ttgtaccgat tctctgagtt acattcacaa gttataataa agaagaattc tatggctatt
      181 ttggcttgtg attgtccaga aaaatgaaag catcggaaat tatacgcaga cgcagacgga
      241 accggaaaaa caaggttacc cgagctgtta aaagcatttc ggcacttcgc atcctttctt
      301 ttattctcat ttgcgtcgca attactttca tcattttatg ttgtgccggt tacgcggtca
      361 ttacacagaa acttccgccg atcgaaaact ttgatgacca ttttgcctcg gcaacggaac
      421 cgacaatcat ctatgaccgc accggtactt accctttact caaattacgc tatccgacac
      481 tggaacatgt ttctctcgca atcgatgaac cggataaacc ctgtttttca tcggatttgg
      541 taaaaattat tgtggcaact catcaaccga atttttggtc ctccgtcggc gcggactggc
      601 atcgctggag caatccggac ccggttacca ttgccgaaag gctggtgcag caaacattcc
      661 caaatcaatt gggaagcgga gtcgaaaaag cgattttgac aaagatcttg gcgacacaaa
      721 ttacaaaaat atacggacct caagcaatat taacctggta tttgaattcg gcttatttcg
      781 gacagcttgc ggtgggcgca gacgccgctg ctcggaccta tctggacaag tccgcttcgg
      841 atttgaatct ttcggaatcg atcttattaa gcgccatctt ggatacgccc tccttgaatc
      901 ccgttgacgc acccggtgtg atgcgcgaat cctatttggc acagattgaa aaattggaag
      961 aaaatgggac attgacagaa gaagagatcc gatcggtccg gagcaccaat tttgtcattt
     1021 tcgaaccgcc gccgttggaa gctgaaatcg cctattctcc cggtctgcag caggcactct
     1081 cttccgcata tgagacgatc ggtcaggaag cgatcgaacg gggcggatat aaaattcgtt
     1141 caaccatcga ttacgatttg caaactcttt tggaatgcct gaccggcatc catcatccgt
     1201 caagctcgga tgtaacagcg caagaaaaaa gctgccctta ttacccgttt gaaacccgga
     1261 cggaagatca aacggcggaa atcgctaaaa ttctctcaca gaatccaatc agcgctgttg
     1321 taatcgatat taaatcaggg cagatgatcg cttcgatcga tgtcggaccg gatggcagcg
     1381 ggaaaagaac ggcgcgcaag gttttttctc agcatgaacc gggaagcaca ttgacgccct
     1441 ttgttgtctt atccgcatta aatcggggaa tctccctttc cagcatggtt tgggatttgg
     1501 ctgaacagag atattatttg ccgtcgggat atcaaaatcc cgacggacgt ttacaaggtc
     1561 cgattcgctt acgaaccgcg gttgacgaag atatcatggg accgattgcc gaaatgcttc
     1621 gggaagtcgg aaccgctccc gtatggaatc aagcgactca attcggaatc accagtgtga
     1681 atgccagaac gccggaaagt attcttttca acggcggttc cgtcaccgta gatatgttgg
     1741 caaatgctta tctgcccttt gcgaaccagg gaagattgat cgggacgatt tacgatgata
     1801 ttccccgtcc gaaaaccatt ttatcgatta cctcacccga gatggctgaa atccctgctc
     1861 aaatgcgttc cggaatttcg ttattggatc cgggcttggc ctacctgatg gtaaatctgt
     1921 tagcggaaga taaacaatcc tatcgctatt tcaatcgtcc agcggggacc aaagtcggaa
     1981 gagtcctcag ttcaaatgat ttttggatga tcggttttac cccggaaatt atgacagtgg
     2041 tttggatcgg aggaacgccg aatggcgccg ggacggatca ggcggcggtt ttgctttggc
     2101 agaccatcat ggctgcggcg gttaagaatc aaccgattac cggttggagt cgcccggaaa
     2161 ccgtaagcca attaatggtt tgtaaccctt cgggaaagct gcctaccgac agttgccccg
     2221 atacggtaag tgaagtcttc ttggcgggaa atgagccaac ggaatatgat gatctatacc
     2281 tcaggatccc tatcaatcga aataataaaa aatggcgacc gcctatacaa aatt
//
LOCUS       DAOMIV010000338         2333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_51125_length_2333_cov_4.165496, whole genome shotgun sequence.
ACCESSION   DAOMIV010000338 DAOMIV010000000
VERSION     DAOMIV010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51125_length_2333_cov_4.165496"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="PKV59_07655"
     CDS             <1..184
                     /locus_tag="PKV59_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46661.1"
                     /translation="ITPIDTVYEAVEKSAYQKGITIDHSELVGCIPKAAIQGIDPDSI
                     KLKDFSPDRILENHLP"
     gene            426..1382
                     /locus_tag="PKV59_07660"
     CDS             426..1382
                     /locus_tag="PKV59_07660"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HQP46662.1"
                     /translation="MTEQRLPSYGGQALIEGVLMRGANHVVASMRAPDGHIEVVHEEL
                     GGIYKSRIRNIPGLRGLISLWDSLGLGLRFLTISANIQTGEDEKIEGPEMVITMIISI
                     VIAVLLFFLAPVWIAHWIESMTNLTQLGGNIIEGFLRLAIVLGYMVIVSRMEEIKRVF
                     RYHGAEHKTINCFDNGLDVTLENVRTQSRLHPRCGTAFMVTVVLISIILFILIGPFTK
                     TLTSRLISRVLLIPVISGIAYEYIRWSARHLNNPLVKILIYPNLMVQKITTADPDDSM
                     LECAISSFLTMLAYENGTMPIEVNKPIKATEYETIGSEKTIL"
     gene            1401..1958
                     /locus_tag="PKV59_07665"
     CDS             1401..1958
                     /locus_tag="PKV59_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQP46663.1"
                     /translation="MNKWQILVSGILIGFLFSALIILFADQQKGKPILLQAESTPGTI
                     QFEVLGAVKNPGIYSSESIIRVIDAVNLAGGLTENADAQNSNLTEKIYDTDRIIIPTI
                     SLVQATTTSIATINNQKRINLNTASLDELMLLPGIGEKKAKDIIEYRTNIQPFSSVSD
                     LLNINGFGEKTIEEFYDQVTVESGG"
     gene            1961..>2333
                     /locus_tag="PKV59_07670"
     CDS             1961..>2333
                     /locus_tag="PKV59_07670"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQP46664.1"
                     /translation="MGIKNDLENKLKDAMREQNEDKKRVYRMALSSIRFEEKTKNKEL
                     DEESVQAIIQKEIKIRNEMIEDAMKAGRDETVNEKKAEISLLEEFLPKQLNDEELSRL
                     ISETVQEVQATGPSDMGKVMKT"
BASE COUNT          789 a          414 c          486 g          644 t
ORIGIN      
        1 cattaccccc atagacacag tttacgaagc ggtagaaaaa tcagcctatc aaaaaggaat
       61 cacaatagat cactcggaat tagtgggatg tatcccaaaa gctgctattc agggaataga
      121 tcccgattcc ataaaattga aagatttttc tccggatcga attctcgaaa atcatcttcc
      181 atagctttat atgttccgat aaatatattt tatttaccct gatgcgtgag tggattcttc
      241 cgtacaaatt ttcaattatt gttcagcggt tactgttttg catcgaaata aatataatca
      301 acttattccg ataatatctg acaaataaat aatcagtcgc atcgactttc ttatgcaaat
      361 taaacgtcaa ataataaata tttccgacat tttagtatat aatccatact tatatggaga
      421 aaaaaatgac agaacaaagg ttaccgtctt atggagggca agcgcttatc gaaggtgtct
      481 tgatgagagg agccaaccat gtagtagctt cgatgcgtgc tcccgatgga catatcgaag
      541 ttgtgcatga agagttggga ggaatctata aaagcaggat tcgaaatatc ccgggactga
      601 gagggcttat cagtttatgg gattctctgg gtttgggctt acggttcctc acaatatcag
      661 ccaacataca aaccggcgaa gatgagaaga ttgaaggacc tgaaatggtc ataaccatga
      721 ttatttccat cgtgattgct gttcttttat tcttccttgc tccggtatgg attgcccatt
      781 ggattgaatc aatgaccaat ctcacgcaac tgggcggaaa tattattgaa ggttttttgc
      841 gccttgccat tgtattggga tatatggtga tagtttctcg gatggaagaa attaaacgag
      901 tgttcagata tcatggtgcc gagcataaaa caatcaactg ctttgataac ggccttgatg
      961 tgacacttga aaatgtgagg actcaatcca gattgcaccc gcgctgtgga accgctttta
     1021 tggttacggt agtgttgatt tccatcattc tgttcatttt aatcggaccg tttaccaaga
     1081 cattgacaag ccgattaatc agtcgggtgt tgttgattcc ggtaatcagc ggaattgctt
     1141 atgaatatat tcgctggagt gcaagacatt taaataatcc gcttgttaaa atcctgattt
     1201 atccgaattt aatggttcaa aaaattacaa cagctgaccc tgacgattcg atgcttgaat
     1261 gtgcgatcag ttctttcttg acaatgttgg cttatgaaaa cggaaccatg ccgattgagg
     1321 taaacaagcc tattaaagcg acagaatatg aaacaatagg atcggaaaag acaatccttt
     1381 aaaattgaac gggtagttat gtgaacaaat ggcaaattct cgtctcagga attttgatcg
     1441 gtttcctttt tagtgcactg attattttat ttgcagatca acaaaaagga aagccaattt
     1501 tattacaggc ggaatcgact cccggtacga ttcaatttga agtattaggt gcggttaaaa
     1561 atccggggat ttactcatcg gaatcaatta tacgggttat cgatgccgta aatcttgcgg
     1621 gcggacttac ggaaaatgcc gatgcgcaaa acagtaatct gaccgaaaaa atatatgata
     1681 ccgaccggat catcattccg acgatcagcc tcgttcaagc gacaacaaca tctatcgcaa
     1741 caatcaataa tcaaaaaaga attaatctca ataccgcaag tttggatgaa ttaatgctgc
     1801 ttcccggaat cggagaaaaa aaagcaaaag atatcatcga atatcgaaca aatattcaac
     1861 ctttctcgag tgtttcggat ttattaaata ttaacggctt cggagaaaaa acaatcgaag
     1921 aattttatga tcaggtaacc gtagaatcgg gaggataaaa atgggaatca aaaatgatct
     1981 tgaaaataag ttgaaagatg ccatgcgcga gcagaatgaa gataaaaaaa gagtgtatcg
     2041 aatggcactt tcatcaatca gatttgagga aaaaacaaag aacaaagaac tggatgaaga
     2101 gagtgtccaa gcgattattc agaaagaaat aaaaattcgc aacgaaatga tcgaagatgc
     2161 aatgaaagcg ggacgggatg aaactgttaa tgaaaaaaaa gccgaaatca gcttactgga
     2221 agaattccta cccaaacagc tgaatgatga agaactttct cggcttataa gtgaaactgt
     2281 tcaagaagtt caggccacag gaccttcgga tatgggaaaa gttatgaaaa cct
//
LOCUS       DAOMIV010000339         2330 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_51231_length_2330_cov_4.895824, whole genome shotgun sequence.
ACCESSION   DAOMIV010000339 DAOMIV010000000
VERSION     DAOMIV010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51231_length_2330_cov_4.895824"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="PKV59_07675"
     CDS             complement(<1..1595)
                     /locus_tag="PKV59_07675"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46665.1"
                     /translation="MLIAWLKEFPNDLWIRYRLAIVLYKSGKTNEAIRLSEIILKYDP
                     EFFEVWALLSVLYPENSEDKKIALSRMKLIKSYQKGKEGSRNKNIFGFFNRGSTEKNL
                     ECIDDVDDFDIITAIHFSLNSDQQKDLESYFRLMKIYTRRWPNAIQFKLLLGDVMNKM
                     GNVEEGTKLIHSTLENDLLGQVANRTWKENNVYKDLWIDSAALSIDVSKIQIPLKVGK
                     KARLDHTINISWSDSDSKLSNRSDELITAADTKDVFMEAPEENDKSLPNPIAIANESM
                     IKREESELSDKEKISPQKEMKTLITSNGNLSSLIGFLKKIFTGRWEKQTETIDSIKEF
                     IYKLDPDDADERFPVYVILSTVAGLTAKYGKNNKDFIDQEMRSAADAIENRNGWNAMV
                     FYPDEFQANSKTVLDPQSIRNSIIKLDESLAQKGSMIGALLIVGGHDVVPFFKLSNPA
                     MDDDLSICSDAPYGSPDSSRYYDQQWQVGRIPGDSSDDPGLLLSQLRSIQNYHIAKYE
                     QEQSRSKRKSAKVRRGAKIKKGV"
     gene            complement(1707..2015)
                     /locus_tag="PKV59_07680"
     CDS             complement(1707..2015)
                     /locus_tag="PKV59_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46666.1"
                     /translation="MINTSITFSIAGIVLFIVSCIAIIMGIVIIISSVFSKNAQEIQK
                     QAAKLSSKGFTDEMSGALGNASTLIRELNTLIETKRGVGLSLVLIGFILAALCYLFLL
                     "
     gene            complement(2034..>2330)
                     /locus_tag="PKV59_07685"
     CDS             complement(2034..>2330)
                     /locus_tag="PKV59_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQP46667.1"
                     /translation="DFREDLYYRLRIVDIEIPPLRERKEDLPELIGFFVKSISAERGV
                     NITKVSEPVLRAFQNYSWPGNIRELKNAIERASIFCEGDTINLCDIPADIAKFE"
BASE COUNT          660 a          496 c          406 g          768 t
ORIGIN      
        1 ttcacccctt tcttgatttt tgcaccgcga cgaacttttg cggattttct ctttgatcgg
       61 gattgttcct gttcatattt tgctatgtga tagttctgaa ttgaacgaag ttgactcaac
      121 aataagccgg ggtcatcgga actgtcaccc ggaattcgtc ctacctgcca ttgctgatca
      181 tagtatcggg atgaatccgg gcttccgtac ggagcatctg aacagattga taaatcatca
      241 tccattgccg gatttgataa cttgaaaaac ggaacgacat catgtccgcc gacgatcaat
      301 aacgctccga tcatcgaacc tttttgtgcg agtgattcgt ctaatttaat aatcgagttt
      361 cggatagatt gcggatccaa aaccgttttg ctgtttgctt ggaattcatc cggataaaat
      421 accatcgcat tccagccgtt tcgattttca atcgcatctg cggcggatcg catttcttga
      481 tcgataaaat ctttattgtt tttcccgtat tttgccgtca atcccgcaac ggtcgataat
      541 attacataaa cgggaaagcg ttcgtccgcg tcatcgggat ctaatttgta aataaattcc
      601 ttaatcgagt caatggtttc agtttgtttt tcccatctcc ctgtaaatat tttttttaaa
      661 aagccgatta atgaagataa attgccgtta gaagtaataa gtgtcttcat ttctttttgg
      721 ggagaaattt tttctttgtc cgaaagctcg gactcctctc gtttgatcat tgattcattc
      781 gcaattgcaa taggattcgg caacgatttg tcattctctt ccggtgcttc cataaataca
      841 tcttttgtat cggcagccgt tattagttca tccgaacggt tcgataattt tgaatccgaa
      901 tcactccatg aaatattaat cgtatgatcc aatctcgctt ttttaccgac tttcaacggg
      961 atttgaattt ttgaaacatc aatcgaaaga gcagccgaat caatccaaag atccttatat
     1021 acgttgtttt ctttccaagt tcggttggca acctggccga gcagatcatt ttccaaggtg
     1081 gaatgaataa gtttggttcc ttcttcaacg ttacccatct tgttcatcac atcacccagc
     1141 aaaagtttga attgaatcgc gttcggccaa cggcgggtat aaattttcat caaccgaaaa
     1201 tatgattcga gatctttctg ttgatcactg ttcagagaaa aatgaatagc cgtaataatg
     1261 tcaaaatcat cgacgtcgtc aatacattcc aaattctttt cggttgatcc tctattaaaa
     1321 aatccgaaaa tatttttatt acgagaacct tctttacctt tttgatagct ttttattaat
     1381 ttcatccggc tgagagcgat tttcttatct tcgctgtttt ccggatacaa aacagacagc
     1441 aatgcccata cttcaaagaa ttccggatca tatttcaaaa taatttcgct taatcgtatg
     1501 gcttcatttg ttttcccgga tttataaaga actattgcca gacggtatct gatccataaa
     1561 tcatttggaa attcctttaa ccaagcaatt aacaattttc ttgcacaatc aaaatttcca
     1621 agttgaaaag ctgcatttga taaatctatt aattgagttc ggttaatctt cgctttttcc
     1681 attcaagggc ctgtcttatt atgaagttaa agtaaaaaga gataacacaa agctgcaaga
     1741 ataaaaccga tcagaaccaa agataatccc accccgcgct ttgtttcgat cagtgtgttt
     1801 aattctcgga ttaaagtact ggcattaccg agcgctccgc tcatttcatc tgtaaaacct
     1861 tttgaagaca acttcgcggc ttgtttttga atctcctgag cattttttga aaaaacactg
     1921 gaaatgatta tgacaattcc cataattatc gctatacaac tgacaatgaa cagaacgatt
     1981 ccggctattg agaaggtaat agaagtatta atcataattt gacgaccctc cctttattcg
     2041 aattttgcga tgtccgctgg aatatcgcat aaattgatcg tatctccttc acaaaaaatg
     2101 cttgcacgtt caatagcatt ttttagctcg cgaatattac cgggccatga ataattctga
     2161 aatgctcgga gaaccggttc agataccttg gttatattca ctcccctttc cgcactgata
     2221 gactttacga aaaacccgat cagctcagga aggtcttctt ttctttcacg taaaggcgga
     2281 atctcgatat cgactatgcg caatctgtaa tataaatcct cccgaaaatc
//
LOCUS       DAOMIV010000340         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_51392_length_2325_cov_5.953304, whole genome shotgun sequence.
ACCESSION   DAOMIV010000340 DAOMIV010000000
VERSION     DAOMIV010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51392_length_2325_cov_5.953304"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="PKV59_07690"
     CDS             <1..1165
                     /locus_tag="PKV59_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46668.1"
                     /translation="TTASVAERLGRRWITCDTSRVAINLAKSRLISNVYPYYKLNDTK
                     QGLKSGFQYHVSKHVTIGTFCRGEEGNGIVLLDKPIEDKDKVRVTGPFTVEAVPSLRV
                     KPFDGNMPKLEVNDAELARTGETGKQAEWRDELKASGIRATGGKMIQFSVVEPMIATR
                     FLHARAEVLEKDGTNKLAYISFGPDYGSLEQRQAEAAIDEARQMKETPDFVIFAAFHF
                     DPEAAKDIDQMEWKGVTILKAQMSVDLLTSDLRKKRSSNQSYWLIGQPDVEVKKLKDG
                     KYTVKLNGFDYYNPVTGEIESKGTKQIAMWFLDTDYDERSIMPDQVFFPEGDEKRDWT
                     KLAKALKAEVNEDALERFSGVTSILFTAGEHKKIAVKIIDNRGIESFVIKGLE"
     gene            1165..>2325
                     /locus_tag="PKV59_07695"
     CDS             1165..>2325
                     /locus_tag="PKV59_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQP46669.1"
                     /translation="MAKAIDTLIINSPYKEPSQHWKYIRETQEFELTEGRRASGYWKA
                     TQRSAGNADDPGEFVTIDTVNIIRPRVRKWREAGYPNVTGITKKLLQYWTDNEQREQK
                     FFFCQIEAVETAIWLAEAPDHEKQGIEIVGDGSDWQRLCLKLATGTGKTVVMAMLIVW
                     QALNKIANNRDNRFSKNILIIAPGLTVKDRLQVLLPGREDNFYQSFNIVNSSMWQELQ
                     QAKIIVTNWHALAPINENAGPKVVKKGPESAEAFCRRVLPEFGNAEGILIINDEAHHC
                     HRPTADEDDESKEEATIWISGIDRIHEARKVLTCYDLTATPFKPTGRNNQGEMLFSWI
                     VSDFGLNDAIESGLVKTPKVAVRDDSTMGSDLKSKLFHIYPEVKEDLNRRAEP"
BASE COUNT          732 a          504 c          548 g          541 t
ORIGIN      
        1 tactaccgct tctgttgctg aaaggttggg aaggagatgg attacttgtg atacttctcg
       61 tgttgctata aaccttgcaa aatcaagact gatatctaat gtatatccat attataaatt
      121 aaatgataca aaacaaggtt taaaatcagg tttccagtat catgtttcaa agcatgtaac
      181 tataggtaca ttttgtcgcg gcgaggaagg aaatggaatt gttctcttag ataaaccgat
      241 cgaagataaa gacaaagtcc gcgttaccgg tcctttcacc gtagaagccg ttccttctct
      301 ccgcgtaaag ccctttgacg gcaatatgcc gaaactcgaa gtaaacgatg cggagcttgc
      361 ccgcacaggt gaaaccggca agcaagccga atggcgagat gaacttaaag caagtggcat
      421 ccgcgcaacc ggcggaaaga tgattcagtt cagcgtagtt gaaccgatga ttgcgactcg
      481 attcttacac gcccgcgcag aagtgctcga aaaagacggc acaaataaac ttgcctacat
      541 ttcctttgga cccgattatg gctcgctgga acagcgtcag gcagaagcag caattgatga
      601 agcacggcag atgaaagaaa cgcccgattt tgtaatattc gcggcctttc actttgaccc
      661 ggaggcggca aaggacattg accagatgga atggaaaggc gtaacgatac tgaaagcgca
      721 gatgagtgtt gaccttttga catcggactt acgcaaaaag cgttcttcaa atcaaagcta
      781 ttggcttatc ggccagccgg atgttgaagt caaaaaactc aaagatggga aatatacggt
      841 aaaactcaac ggctttgatt actacaatcc tgtcaccggt gaaatcgaat ccaaaggcac
      901 aaagcaaatt gccatgtggt ttctcgatac cgattacgac gaacggagca ttatgcccga
      961 ccaggtattt ttccccgaag gtgatgaaaa acgcgactgg acaaaactcg ccaaagccct
     1021 aaaagccgaa gtcaacgaag acgcgctgga gcggtttagc ggtgtaacat cgatactgtt
     1081 tactgcggga gaacataaga agatagcggt caaaattatt gataaccgcg gtatcgaaag
     1141 ctttgtaata aaggggttgg aataatggca aaagcaatcg acacgcttat tatcaatagt
     1201 ccatataaag agccttcaca gcactggaag tatatccgcg aaacgcagga atttgagtta
     1261 acagaaggca ggcgcgcctc cggctactgg aaagcgacac agcgttcagc aggtaatgcc
     1321 gatgaccctg gcgagttcgt tactattgat acggtaaaca tcattcggcc gcgtgtccgt
     1381 aaatggcgag aagccggtta tcccaatgtt acgggaatta ctaaaaaact gcttcaatat
     1441 tggactgata acgaacagag ggaacagaaa ttcttttttt gtcagattga ggcggtagaa
     1501 accgccattt ggcttgccga agcgcctgac cacgaaaagc aaggcattga aatcgtcggt
     1561 gatggcagcg actggcaaag gctgtgcttg aaacttgcaa ccggtacagg aaaaactgtt
     1621 gtcatggcaa tgctcattgt ttggcaggca ctgaataaaa ttgccaataa ccgtgataac
     1681 cgcttttcaa aaaatatact aatcatcgcg cccggactta ctgttaaaga ccgcctgcaa
     1741 gttcttttgc ctggcagaga agataatttt tatcaatcat tcaacattgt caattcttca
     1801 atgtggcagg aattacagca ggcaaaaatc attgttacca actggcatgc ccttgcaccg
     1861 attaacgaaa atgcaggtcc aaaagtcgta aaaaaaggac cggagagtgc ggaagcattt
     1921 tgccgacgag ttttgcctga atttggaaat gcagaaggta ttcttatcat caatgatgag
     1981 gctcatcact gccaccgccc gacagccgat gaggatgatg aatcaaaaga agaagcaacc
     2041 atttggataa gcggaataga ccgcattcat gaagcgcgga aagtactcac ctgttacgat
     2101 ttaactgcta caccgttcaa acctaccggt agaaacaatc agggagaaat gcttttttcc
     2161 tggattgtca gcgatttcgg actgaatgac gcaatcgaaa gcggattggt taaaaccccc
     2221 aaagtcgcag tccgtgatga ctcaaccatg ggttccgatc tgaaatcaaa actattccac
     2281 atttatccag aagtcaaaga agacttaaac cggcgtgctg aaccg
//
LOCUS       DAOMIV010000341         2315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_51730_length_2315_cov_3.702212, whole genome shotgun sequence.
ACCESSION   DAOMIV010000341 DAOMIV010000000
VERSION     DAOMIV010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51730_length_2315_cov_3.702212"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PKV59_07700"
     CDS             <1..455
                     /locus_tag="PKV59_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP46670.1"
                     /translation="HLSVLDNVMLAQKIVRKRSQKEAYETAMLLLEKVGIPDKASAYP
                     LQLSGGQQQRVAIARALAMNPKIMLFDEPTSALDPEMIQEVLEVMIQLAKEGMTMVVV
                     SHEMGFARAAADRCILMDEGKIVEEAPKDELFKNPKEERTKLFLSKIL"
     gene            622..1416
                     /locus_tag="PKV59_07705"
     CDS             622..1416
                     /locus_tag="PKV59_07705"
                     /inference="COORDINATES: protein motif:HMM:NF039614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HQP46671.1"
                     /translation="MYKFEKAVVRTPADSFAEGITPGLFGKADPELAKIQHKKYIQAL
                     RQCGLEVIVLEPDNDFPDSCFVEDPVIVTDRVAILTNFHAPTRKGEVDRILPTIKDIY
                     GSHIERIEGPGTVEGGDICQVENHFFIGLSQRTNEEGACQLAAILKKYGFTSSIISIR
                     ALPEILHLKTCMSYIGENTFILQKAILKESELAPFKKIVVPDNEGYASNCIRVNDTVI
                     LPEGFDETIKQTRAAGFNVLETPMSEFMKQDGGLSCLSLRIPKLNL"
     gene            complement(1629..2144)
                     /locus_tag="PKV59_07710"
     CDS             complement(1629..2144)
                     /locus_tag="PKV59_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46672.1"
                     /translation="MGASEGDMPLCSSLNLQLEWLAAGTDSNVEWQWNGGHVPSEIFS
                     ESFALYVDQMYAQYASAEQKVDTVTIAAPAVQTANGTAASATGMDISGWVSASDISSV
                     SFSLADGMAYRNKGASKAVPGFDVIDYGQEDYVFGDKDSDARHWDTFVNKIFQSPEYA
                     GVLANLFNEAK"
BASE COUNT          659 a          499 c          515 g          642 t
ORIGIN      
        1 ctcacttatc cgtattggat aatgttatgt tggcacaaaa gattgttcgt aaacgcagtc
       61 aaaaggaagc ttacgaaaca gccatgcttc ttttggaaaa agtgggaatt ccggacaaag
      121 cttcggcata cccgcttcag ttatccggcg gacaacaaca acgggtggcg attgctcggg
      181 ctttagccat gaacccgaaa attatgttgt ttgatgaacc gacaagcgca ttggatcccg
      241 aaatgattca ggaggtactg gaagtcatga ttcagttggc gaaagaaggc atgacgatgg
      301 tcgtcgtatc tcatgaaatg ggtttcgccc gtgcggcagc ggatcgctgc atcctcatgg
      361 atgagggaaa aattgtggaa gaagctccaa aagacgaatt atttaagaat cccaaggaag
      421 aacggacaaa gttattcctg agcaaaattt tataaagcaa ccaggcaggt taggaatcaa
      481 aaaagcccat ttatccgggc ttttttgatt ttttttatcg catcatttgg ttttcgataa
      541 tctggtatga tagtaacgtt tcgtgttcga ccaaagaaca gaaaaatctt ttaaattttt
      601 tgaatgccaa ggaaagggat tatgtataaa tttgaaaaag cggttgtccg aactccggcc
      661 gattccttcg cagaaggaat tacacccgga ttattcggaa aagcggatcc ggaattagcc
      721 aaaatacaac ataaaaaata tatccaagct ttacgtcaat gcggtctcga agtaatcgtc
      781 ttagagccgg ataacgattt tccggattct tgtttcgttg aagacccggt aatcgtcacc
      841 gatcgggtcg ctatcttaac aaattttcac gccccgacac gaaaaggaga agtcgatcga
      901 attttaccga ctattaaaga tatctacgga tcgcatatcg aacgcatcga gggaccggga
      961 acggttgagg ggggcgatat ttgccaagtg gaaaatcact ttttcatcgg attatctcaa
     1021 cgaacgaatg aagaaggtgc ttgccagctt gccgcaattc tgaagaagta tgggtttacc
     1081 agcagcatca tcagtattcg tgctctgccg gagatattac atctgaaaac atgcatgtcc
     1141 tacatcggag agaatacatt tattctgcaa aaagccattc tgaaagagtc ggaattagcg
     1201 cctttcaaga aaattgtcgt tcccgataac gaagggtatg catcgaactg tattcgggtg
     1261 aatgatacgg ttattctgcc ggaaggtttt gatgagacga ttaaacagac ccgcgctgcc
     1321 ggttttaatg tgttggaaac accgatgagt gaatttatga agcaggacgg cggtctgagt
     1381 tgcctttcct taagaattcc gaagctcaat ctgtaaaata attcggaatc acatatcatt
     1441 ctcttatctt tcaggatggg gagtgtgatt cttttgacaa acaaacattt ttccgccgag
     1501 ccgacaattt atcattgcca agctcggcgg tcttgttttc tcatttccgg attggatcga
     1561 tactcttatt ctttctgcta tttttcaaaa tagactatct gaaattgtcg gcttcatcca
     1621 agtgaacttc atttcgcttc gttgaacaag tttgcgagta cgcctgcata ttccgggctt
     1681 tggaagatct tgttcacaaa agtatcccaa tgccgtgcat cactgtcctt atccccgaat
     1741 acataatcct cttgtccgta atcaatgacg tcgaaaccgg gcaccgcttt gctggcgccc
     1801 ttattccgat atgccatccc atctgcgaga gaaaaggata cagaggaaat gtcagaggca
     1861 ctaacccagc cggagatatc cattccggtt gccgaagcgg ccgtaccatt ggcggtttga
     1921 actgccggcg ccgctatggt aaccgtatcg actttctgtt cggcggaagc atattgggca
     1981 tacatctgat ctacatatag tgcgaaggat tcactgaata tttcggacgg aacatgtccc
     2041 ccgttccatt gccattcgac attactgtcg gttccggctg ccagccactc gagctgcaaa
     2101 tttaaactcg aacaaagcgg catatcgccc tctgaggcac ccatcagcat acgtgtccag
     2161 gtcggatttt tggttccctc ggcaccgata tattcggtta ttaactgttt ttttattttt
     2221 gtttataatt cacatcatat acaaataata agcaagaaaa ataaaaaaat tcaaaacacc
     2281 tggttttcaa agggattcat ttttccccgt cacaa
//
LOCUS       DAOMIV010000342         2313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_51805_length_2313_cov_3.085474, whole genome shotgun sequence.
ACCESSION   DAOMIV010000342 DAOMIV010000000
VERSION     DAOMIV010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51805_length_2313_cov_3.085474"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..448)
                     /locus_tag="PKV59_07715"
     CDS             complement(224..448)
                     /locus_tag="PKV59_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46673.1"
                     /translation="MSIKLLDNSLELEIFLDKEDSEFEDNICLRFIEDCPEDEKVFYG
                     GETNIYITRDQALQIVKALGAVLEEANESN"
     gene            601..990
                     /gene="gcvH"
                     /locus_tag="PKV59_07720"
     CDS             601..990
                     /gene="gcvH"
                     /locus_tag="PKV59_07720"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQP46674.1"
                     /translation="MNIHPNYFYSKNDEWVSVEGNKATIGISDYAQDSLSDIVFVEIS
                     VSVGDSLAAEDNIGTIESVKAASDIYTPVAGTVSEINNKVVDSPELLNRDPFGEAWLI
                     KLSSDTGFDTSHLMDADAYRKYCEDRG"
     gene            994..>2313
                     /gene="gcvPA"
                     /locus_tag="PKV59_07725"
     CDS             994..>2313
                     /gene="gcvPA"
                     /locus_tag="PKV59_07725"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQP46675.1"
                     /translation="MFIPHTDADRESMLAAIGKKDLNELFDVVPEKYRFPKLNLQPGL
                     TEPEVTFLLDEISSANLSLDHTACFTGAGAYHHYIPAAIDNLISRGDFYTAYTPYQPE
                     ISQGTLQAVYEYQSLMARLTGMEAANASHYDGATACAEAGILAYHQYHGKRKKILVSA
                     GLHPHYLLTLKTYLQDFDGMQIECPKAASLDEINPQRLEEKIDTDTAVVLVQYPDFFG
                     QIQDFSELAKAVHEKGSLLCVCINPIALGLLKTPGEMGADIVVGEGQPLGIPLSYGGP
                     YLGIFTTRKELIRKVSGRIVGETVDNRGQRAYVLTLTAREQHIRREKATSNICSNQGL
                     NALTAAIYLSLLGRDGLQAVAKLCYDKAHYAAKEISKIHGYRILNQRPFFHEFVVSCP
                     QAPSDINQHLLDHGMIGGYDLSEEYPMLGNAMLIVVTEMINKEDIDDL"
BASE COUNT          660 a          524 c          515 g          614 t
ORIGIN      
        1 gccggttccg gaattcgaac agcggatttt ccgccagaac ggggacagtt aaacaatacg
       61 cttgctcatg ccgttgaaag aatcttcgga ttactggtaa cggacgaggg ccatcagttg
      121 atcgatacat ctgtttttca atcatatttg aatcgaaaag aaaagcactg aaaataaaaa
      181 agacccggta gttttcatga agataccggg ctttttttcg aaatcaattt gattcatttg
      241 cttcttccag aacagcgccc aacgctttaa cgatttgcaa cgcttgatcg cgcgtaatat
      301 aaatattcgt ttcaccgccg taaaaaactt tctcatcttc cgggcagtcc tctataaatc
      361 ttaggcaaat attgtcctca aattccgaat cttctttatc gaggaaaatt tccaattcca
      421 gactgttatc caagagttta atggacatat attcctcctt tcgacaatcc tctccttcca
      481 ttattatacc caaaaggaat gacgatgtct tccttaattt gttaagtgtc tgcgaattcg
      541 ctataaatat tattcgaatc cacttataat aaagaaaaat gaataagcgg agggaaatca
      601 atgaatattc atccaaatta tttttattca aagaatgacg aatgggtttc ggtcgaagga
      661 aataaagcca caatcggaat ttcggattat gcgcaggatt cgctttctga cattgtattt
      721 gtcgaaatca gcgtttccgt cggggattca cttgcggcgg aagacaatat cggaaccatt
      781 gaatccgtca aagccgcatc ggatatctac actcccgtcg cgggaaccgt ttcggagatt
      841 aataacaagg tagtcgattc gcccgagctg ctaaatcggg atcctttcgg agaagcctgg
      901 ctgatcaaat taagcagcga tacagggttt gatacttctc atttaatgga cgcagacgca
      961 taccgaaaat attgcgaaga tcgaggctga accatgttta ttccgcatac ggacgccgat
     1021 cgtgaaagta tgctggcagc gatcgggaaa aaagatctga acgaattatt tgatgtcgtt
     1081 ccggaaaaat atcgttttcc gaaattgaat ttacaaccgg gattgacgga accggaagtc
     1141 acctttctgc tcgatgaaat ttcatcagcc aatctatccc tggatcatac agcctgtttc
     1201 accggtgccg gtgcttatca tcattatatt cccgccgcaa tcgataatct catcagtcgc
     1261 ggcgattttt ataccgctta taccccttat caaccggaaa tctcgcaggg tacattgcaa
     1321 gctgtttacg aatatcaaag tctgatggct cgattaaccg gcatggaagc cgccaacgcc
     1381 tctcattatg acggtgcgac agcctgcgcc gaagccggta ttttggctta tcaccaatac
     1441 cacgggaaaa ggaagaaaat tttggtatct gcgggactgc atccgcatta cctgctaacc
     1501 ctgaaaacct atctgcagga tttcgacgga atgcagatcg aatgtccgaa agccgcatcc
     1561 cttgatgaaa ttaatccgca acggttggaa gagaaaatag atacggatac cgcggtggtc
     1621 ttggttcaat atccggattt cttcggacaa attcaggatt tcagcgagct tgcaaaagcc
     1681 gttcatgaaa aaggatcctt gctctgtgtc tgtatcaacc ccattgcgtt gggactgctg
     1741 aaaactcccg gtgagatggg cgccgatatc gtcgtcggtg aaggtcagcc gctgggaatt
     1801 ccgctttctt acggcggccc ttacctcgga atattcacca cacgaaaaga attaatccgc
     1861 aaagtgtccg gaagaatcgt cggtgagacg gtagacaatc gagggcaacg ggcctatgtg
     1921 ctgacattaa cagcccgaga acaacacatt cggcgggaga aagccacctc caatatttgt
     1981 tccaatcagg gattaaatgc attgactgca gccatttacc tttcattgct cggccgggac
     2041 ggattacagg cggtggcaaa attatgctac gataaagcgc actatgccgc aaaggagatc
     2101 agtaagattc acggatatcg tatcctgaat caacggcctt tcttccatga attcgtcgta
     2161 tcctgtcctc aggcgccgtc ggatataaat caacatttac tggatcacgg aatgatcggc
     2221 ggatatgatc tctccgaaga atatccgatg ctggggaatg ctatgctgat tgtggtgacg
     2281 gaaatgatca ataaggaaga tattgatgat ctc
//
LOCUS       DAOMIV010000343         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_52304_length_2298_cov_3.729380, whole genome shotgun sequence.
ACCESSION   DAOMIV010000343 DAOMIV010000000
VERSION     DAOMIV010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52304_length_2298_cov_3.729380"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PKV59_07730"
     CDS             complement(<1..506)
                     /locus_tag="PKV59_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46676.1"
                     /translation="MAYPLSSDVRAGETTLASQYNNLRADAIRLGGDSANTATLQQLL
                     STYTSYLKLSASGNQINLTASSENPCSLMIAGCPSTITESIHLAISPADFPQSAEIWL
                     FARKNPLTPGFEMIARLSEIGSGDETGIAHCIWNAERQQIDELKNLFEYQISRKCIQP
                     EVCQGRLT"
     gene            complement(646..1164)
                     /locus_tag="PKV59_07735"
     CDS             complement(646..1164)
                     /locus_tag="PKV59_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46677.1"
                     /translation="MNDRIWVAGENCDLILKHPAHESLPLIGYRTPADALGPRIRIHY
                     EVYWPDESETQTEPIEIRHLWFTVLVADPMLCPDGSWYPYSSREVRSRLNAILLEKKN
                     IRLTTSAGTITGLYGTDHVVIDTIYAGAHTLEIHLSTQVLSDLPAVPIDMWLDEVGVE
                     SSAWGVAIWRAE"
     gene            complement(1161..1799)
                     /locus_tag="PKV59_07740"
     CDS             complement(1161..1799)
                     /locus_tag="PKV59_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46678.1"
                     /translation="MESFDDFKSQILAILGDENRKRFSDEMLKTGLRSALTDYDRYCP
                     CVREIISSVEAIDEQTFTVLPQPAANQQLYGILWIDPTTKQIIEPSFIATPSDSGLRI
                     RPDRKIPLSVGDPISLRVREAHSIQGLDSSAITSVPIMHRSFLCEGAAGYVLQVRASA
                     ITEVFGKRPEDSARLLQLSRELLDRFHAVLADLSRTGGEWAGAVFPSKGFEI"
BASE COUNT          540 a          594 c          553 g          611 t
ORIGIN      
        1 aaagtcaacc gtccttgaca aacttccggt tggatacact ttcgggagat ttgatattcg
       61 aacagattct tcaattcgtc gatttgttgg cgttcggcat tccaaatgca atgggcgata
      121 cctgtttcgt cgcccgatcc gatttccgat agacgtgcga tcatttcgaa tcccggcgtg
      181 agcggatttt tacgggcaaa aagccagatt tccgcgcttt gtggaaaatc cgccggggaa
      241 atcgccagat ggatcgattc cgtaatcgtc gacggacaac cggctatcat caatgaacag
      301 ggattttcgc ttgatgcggt aaggttgatt tggtttccgc tcgcgcttaa tttcagatac
      361 gaagtataag tggacagtaa ttgctgtaag gttgccgtat tcgcggaatc cccgcccagg
      421 cgaattgcat ctgcgcgcag attattgtat tggctggcga gcgttgtctc gccggctctg
      481 acgtccgatg aaagaggata tgccattatc gttttctccg tttttttgtt ttttgtttca
      541 ggatctttcc gcatgggaaa gacagaattt tctcttcgag tttttcaggc ttccgaaact
      601 gccttcattt tctcggagag cggactgtat cggccggatc cgcgcttatt cggctcgcca
      661 gatcgccacg ccccacgcgc tggattccac cccgacttca tccagccaca tatcgatcgg
      721 cacagcgggc agatccgaca aaacctgcgt tgaaaggtgg atctccaaag tgtgcgcacc
      781 ggcatagatt gtatcgatca cgacatgatc cgtcccgtat aaaccggtaa tcgttccggc
      841 tgaagtcgtc aggcgaatat tcttcttttc caacaggatc gcattcaacc gggagcgaac
      901 ttcccgtgaa gaatacggat accaactgcc gtcagggcaa agcatcggat cggcgaccag
      961 caccgtaaac cataagtgcc ggatttcaat cggttcggtt tgtgtctcgg attcatcggg
     1021 ccaatatacc tcataatgaa tacggattcg cggcccgagc gcatccgccg gtgttcgata
     1081 accgatcagc ggcaggctct catgcgccgg atgcttcagg atcagatcac agttctcgcc
     1141 ggctacccat attctgtcgt tcatatttca aaccctttgg acgggaaaac agctcccgcc
     1201 cactcaccac ccgttcgact caaatccgct aaaaccgcat gaaaacgatc caaaagttcg
     1261 cgggaaagtt gcagcagccg agccgaatcc tccggacgct ttccgaatac ttccgtgatt
     1321 gccgaagctc tgacttgcag gacatatccg gcggctcctt cgcacaggaa cgaacgatgc
     1381 atgatcggga cggaagtgat tgccgaactg tccaatccct gaatcgaatg cgcttcccgc
     1441 acgcgcaaac tgatcggatc accgacggat aaagggattt tacgatcggg gcgaatccgt
     1501 aagccggagt ccgacggggt tgcgatgaaa gacggctcga tgatttgctt tgtagtggga
     1561 tcgatccaca agattccata cagttgctga tttgcagccg gctgcggcaa aacggtaaag
     1621 gtttgttcat caatcgcttc gactgatgaa atgatttccc gtacgcaggg acaatatcga
     1681 tcataatctg tcagtgccga tcgaagaccg gttttcagca tttcatcgct gaatcgtttt
     1741 ctgttttcgt ctccgagaat cgccagaatt tgagatttaa aatcgtcaaa agattccatt
     1801 tttccaccat attcttttgt tgtgttgagg atctgtgtat aaagaaggcg gttatatgcg
     1861 gtttatccac aggcttatcc acatgttttt cgcacttctc gcacagtttc catctcgaaa
     1921 gagagagatt tttgtacagg gctcgcgtgg atatacggtc gcttttttcc ctcaccctaa
     1981 ccctctcccg atgggcgagg gtttttggct tcggcgtccc ttttctaaaa aaattaatgt
     2041 aggggtcggt tccaaaccga cccttctttg taaccatgcc cgaaaaggca tccgtcgggg
     2101 acgacgtccc gctccgaaca aagtaaggat tcaacaattt gattttgctg attagatgta
     2161 atatcgtata caaatctcgc attccctcac cctaaccctc tccccaaggg cgagggtttt
     2221 tggtttcggc gtcccttttt aaaaaaatta atgtaggggt cggttccaaa ccgacccttc
     2281 tttgtaacca tgcccgaa
//
LOCUS       DAOMIV010000344         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_52400_length_2295_cov_4.100000, whole genome shotgun sequence.
ACCESSION   DAOMIV010000344 DAOMIV010000000
VERSION     DAOMIV010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52400_length_2295_cov_4.100000"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PKV59_07745"
     CDS             <1..336
                     /locus_tag="PKV59_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46679.1"
                     /translation="QLLRLTPYFEGIYGAQDDYIRFSKRMVIQRIIRENKLSGPEFVG
                     FGDGFVEIEDTKSVDGTAVGVASNEQTREGIDEWKRERLIQAGADLIIPDFRCSDELI
                     KILFPKESR"
     gene            349..1329
                     /locus_tag="PKV59_07750"
     CDS             349..1329
                     /locus_tag="PKV59_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46680.1"
                     /translation="MAYKTFDRSKLNIKPISERIHDLHLPQMLIPLDAPVPPFSDPAI
                     PKIAQAMIKAHENNAPVILMMGAHLLRKGMAPMIIDLMEKGLINHIGLNGAGCIHDYE
                     LARIGATTESVARYIHEGQFGLWQETGELNDIINHGSDAGLGIGEAVGKAIVENDYPY
                     RKTSLLARAWELGVPVTVHVSIGSDIIHEHPNCDGAKLGKASYQDFLVFTNTIDHLEQ
                     GVYLSYGTAIMGPEVYLKALAMARNVAFQEGRQICHFTTAVFDLQDLGTDWHREISKD
                     QAAYYFRPYKTILVRTVADGGVSYYVRGDHAVTFPNLYHSIIDHFQDGRE"
     gene            1326..>2295
                     /locus_tag="PKV59_07755"
     CDS             1326..>2295
                     /locus_tag="PKV59_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQP46681.1"
                     /translation="MNSKRIAEILKIYPDLHILVAGDYFLDQYLMINRDLAEISLETG
                     LECHQVTEIRNSPGAAGTVANNLVALDVSVNALSAIGKDGRGFDLKTELLKRNIDTRA
                     LIESPLIMTPTYTKPMVQGKNEPLHELERIDIKNRSHLPIEIEDTIIENLENSFQLAD
                     GIVLVDQVQEENCGVLTDRVRNQIAALALKYPDKPVLADSRLRSAAFKNVTVKCNLSE
                     ALNVTGESDVERAAKKMVAGGARSAFITMGEKGIFAYSNGKGVTVPAVRVEGPIDICG
                     AGDSAAAGILSALCAGASFEEAAIIGNLTASITILQIGTTGTASREG"
BASE COUNT          640 a          518 c          566 g          571 t
ORIGIN      
        1 caattactgc gtttaacccc ttatttcgaa ggaatatacg gtgcccagga cgattacatt
       61 cgtttttcga aacgaatggt aatccagcgt ataattcggg aaaataagct gagcggaccc
      121 gaatttgtcg ggttcggcga tggatttgtc gaaatcgaag ataccaaatc ggtcgacggc
      181 acggcagtcg gcgtcgccag caatgaacaa acacgtgaag gaattgacga atggaaacgg
      241 gaacgcttaa ttcaggcggg tgcggatttg attattcccg attttcgctg ttccgatgaa
      301 ttaataaaaa tattatttcc gaaagaaagc agataaggaa gggttcttat ggcttataaa
      361 acttttgatc gttccaaatt aaacattaaa ccgatctccg agcgaattca tgatcttcat
      421 ttgccgcaaa tgttaattcc cttagacgcg cctgtccctc cgttttcgga tcctgcgatt
      481 ccgaaaattg cacaggcaat gatcaaagcg catgaaaaca acgccccggt tattctgatg
      541 atgggcgcac atttactccg gaaggggatg gcaccgatga tcattgatct gatggaaaaa
      601 gggctgatca atcatatcgg attgaacgga gccggttgta ttcatgatta tgaattggct
      661 cgaatcggag cgacaaccga aagtgtcgcc cgttatattc atgaaggaca atttggcctg
      721 tggcaggaaa ccggcgaact gaatgacatt atcaatcacg gttcggatgc cggattgggc
      781 atcggcgaag cggtcggaaa agcaatcgtt gaaaacgact atccctatcg aaaaaccagt
      841 ttgctggccc gcgcctggga actcggagtt ccggtgaccg tccatgtcag catcggttcg
      901 gatattattc atgaacatcc caattgtgac ggggcaaaat taggaaaagc ttcctatcag
      961 gactttctgg tctttaccaa taccatcgat catctcgaac agggcgtata tctcagttac
     1021 ggtaccgcca tcatgggacc tgaggtctat ctaaaagcct tggcaatggc tcggaatgtc
     1081 gcctttcaag aaggacgaca aatctgccac tttaccaccg ctgtttttga tttacaggat
     1141 ttaggcaccg actggcaccg ggagatttca aaagaccaag ctgcttatta cttccgtccc
     1201 tataaaacta ttttagtgag aaccgttgcg gatggtggcg taagctacta tgtacgggga
     1261 gatcacgccg tgactttccc gaacctctat cattcaatca tcgatcactt tcaggacggc
     1321 cgcgaatgaa ttcgaaacgg attgccgaaa tattaaaaat ttatcccgat ttgcacattc
     1381 tggttgcggg agattatttt ctggatcaat atttgatgat taaccgcgat ctggcggaaa
     1441 tatccttgga aaccggtttg gaatgccatc aggtcacaga gattcgcaac agtccggggg
     1501 ctgccggaac cgtggcaaat aatcttgtcg cattggatgt ttctgtcaat gctctcagcg
     1561 caatcggtaa ggacggccgc ggttttgatt taaaaaccga attactgaaa cggaacatag
     1621 atacccgcgc tctgatcgaa tccccgctga ttatgacccc cacatatacc aagccgatgg
     1681 ttcaggggaa aaatgaaccc ctccatgaac tggaacggat cgatataaaa aatcgttctc
     1741 atcttccgat tgaaatcgaa gacacaatca ttgagaatct tgaaaattct tttcaactcg
     1801 ccgatggaat cgtgcttgtc gatcaggttc aggaagaaaa ttgcggcgtc ttaacggatc
     1861 gggtcagaaa tcaaatcgca gctctggcat taaaatatcc cgacaaacct gttctggcag
     1921 attcgcgcct gcgaagcgcg gcttttaaaa acgtaacggt aaaatgcaat ctatccgaag
     1981 cgttgaatgt taccggcgaa tccgacgttg agcgggctgc caaaaaaatg gtcgccggcg
     2041 gcgctcgcag cgcttttatt acgatgggag aaaagggtat ttttgcctat tcaaacggaa
     2101 aaggtgtgac cgttccggca gtcagagttg agggacctat cgatatatgc ggtgccgggg
     2161 atagcgccgc cgccggaatt ctgagtgcgt tatgtgccgg agcaagcttt gaagaagcag
     2221 caattatcgg taatctgacg gcatccataa caatcctgca gatcggcact accggtacag
     2281 ccagtcgaga aggga
//
LOCUS       DAOMIV010000345         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_52404_length_2295_cov_3.641964, whole genome shotgun sequence.
ACCESSION   DAOMIV010000345 DAOMIV010000000
VERSION     DAOMIV010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52404_length_2295_cov_3.641964"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PKV59_07760"
     CDS             <1..1155
                     /locus_tag="PKV59_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQP46682.1"
                     /translation="MVYEAARGANSQYFNLLNINAAQVGLVFGIGEFLGYMLRLFSGI
                     ISDKSGKYWLFIFIGYGMLIVIPLMGFTKNWNILVVLILLERIGKSLRSPAKDTIVSG
                     VAENQVGIGFAFGLQEALDQIGAFLGPLIFTLVFYLAGKDGISVYQLGYKVLVIPFIL
                     LMFFVTFAYRKIEREHLVPSLDAKEFQNEKLGSTFWAYTAFTFFCTLGFVNFSTVGFH
                     LKANNLMSDGNITLLYAIAMIVDALTALIVGRAYDSQKKKTGRKTGGLLVLALIPFIT
                     FLLPFLTLSYSTTLIIAGMVIFGIVMGTHETIMRSAIADIAPFHKRGTSYGVFNTGYG
                     LALLGGSSLMGWFYNLDKIGLIIGLTCLSEFIAILFYVKMNHLVKASTNK"
     gene            1362..>2295
                     /locus_tag="PKV59_07765"
     CDS             1362..>2295
                     /locus_tag="PKV59_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46683.1"
                     /translation="MTSSQIVEIQQDRFSRQLRHVLRYSEFYRNFYRKAGIDIERPEL
                     IQLKDLPVIDKDLMMDHFDEFVCNKQLKKSKLEDFIKDPNSVDKKYKDEFKVIHTSGS
                     SGRIGIFVYGANDWAIAEAAVYSRISKNKLHFIGKAKHAFIGVTDGHYAGISLAKSGP
                     KWLFDFLPVDMNQPLHPSLVRLNDFQPECLSGYSSGVYLLALEQLKGNLKIHPTRILC
                     SADPLTSEMRETIRNAFGIMPYNYYAASESLCMGIQCDRFNGIHLFDDLHIFEIIKEN
                     GEEAKPGEPGNLVMTNLYNKTQPLIRYRMNDNLIP"
BASE COUNT          659 a          436 c          480 g          720 t
ORIGIN      
        1 atggtttatg aagctgccag aggcgcaaac agccaatatt tcaatttatt aaatatcaat
       61 gccgcacaag tcggactggt ttttgggatc ggtgaatttc tcggttatat gctgcgtttg
      121 ttctcgggaa tcatttcaga taaaagcgga aaatattggc tttttatttt tatcggttac
      181 ggaatgttaa ttgtgattcc tttgatgggc tttacaaaga actggaatat cctggttgtt
      241 ttaattctac tggagagaat cggaaagtct cttcgcagtc cggcaaaaga taccatcgtt
      301 tccggagtgg cggaaaacca agtcggaatc gggtttgcgt tcggtttgca ggaagccttg
      361 gatcaaatcg gcgcatttct cggcccatta atttttacac ttgtctttta tttggccgga
      421 aaagacggaa tttccgttta tcaactcgga tataaagtgc tggtaattcc tttcattcta
      481 ttaatgtttt ttgtcacttt tgcttaccgg aaaatcgaac gggaacattt ggttccttct
      541 ttggacgcca aagaattcca aaacgaaaaa ttgggttcga ctttctgggc ttacacagct
      601 tttacatttt tctgcacttt ggggttcgta aatttcagca cggttgggtt tcatctgaag
      661 gctaataatt tgatgtcgga tgggaatatc actcttcttt atgcgatagc gatgatcgtt
      721 gatgccctta cagctcttat tgtcggcaga gcttacgatt ctcagaaaaa gaaaaccggc
      781 agaaaaacgg gcggattact ggtgctcgct ttaattccat ttattacttt tcttttgcca
      841 ttcctcactt tgagttattc aaccaccttg atcatcgccg gtatggtaat ctttgggatc
      901 gtcatgggta cacacgaaac aattatgcgg tctgcgatag cggatatcgc tcctttccac
      961 aaacgtggga ccagttatgg cgtgttcaat accggctacg gtttagctct gttaggcggc
     1021 tcttctttga tgggctggtt ctacaattta gataaaatcg gcttgatcat cggtttgacc
     1081 tgtctttccg aatttattgc gattttgttt tacgtcaaaa tgaatcattt agtaaaagca
     1141 agtaccaata aataagaaat aatccggata ttttttagaa caaagccttg tttttgtaag
     1201 cagggctttg ttcattaata gtcagcgaaa aaaatcgatt ctgcgtctcg ttaattttgc
     1261 aatatgatta aatcaaaaat tctatgaatg taaaagcttc tttttcttca tctgttttga
     1321 atattattcg aatgatccgt tgcctcggat ctgccgaaaa aatgacatca agtcagatcg
     1381 ttgaaataca acaagatcgg ttctcccgac aattaagaca tgtactcaga tattcagaat
     1441 tctatcgaaa cttttatcga aaagcaggaa ttgacattga aagaccggaa ctgattcaac
     1501 tgaaagactt accggtgatc gataaagact tgatgatgga tcatttcgac gaattcgtct
     1561 gcaataaaca attaaaaaaa tcaaagctgg aagattttat caaagaccct aattctgtcg
     1621 ataaaaaata taaagatgaa tttaaagtga ttcatacatc ggggtcatcg ggaagaatcg
     1681 gaatattcgt gtatggagca aatgattggg cgattgccga agcagccgta tattcaagga
     1741 tttctaaaaa caagctccat ttcatcggaa aagcgaaaca cgctttcatc ggtgtgaccg
     1801 atggacatta tgcgggaatc agtctggcga agtccggacc caaatggtta tttgattttt
     1861 taccggttga tatgaaccag ccgctccatc cgtcactggt taggttgaat gattttcaac
     1921 ccgaatgtct cagcggatat tcttccggag tttatctgct cgctctcgaa cagttgaaag
     1981 ggaatctgaa aattcatccg actcgaattt tatgttcggc ggatcccttg acttccgaga
     2041 tgagagaaac aattcgcaac gccttcggca ttatgcctta taactattac gcggcatcgg
     2101 aatccttatg catgggaatt caatgtgacc gatttaacgg cattcattta ttcgatgatt
     2161 tgcatatttt tgaaattatt aaagagaacg gcgaagaagc aaagcccggt gaacccggta
     2221 atctggttat gaccaattta tataacaaaa cacagccact catccggtac agaatgaatg
     2281 acaacttaat tccat
//
LOCUS       DAOMIV010000346         2292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_52497_length_2292_cov_4.288780, whole genome shotgun sequence.
ACCESSION   DAOMIV010000346 DAOMIV010000000
VERSION     DAOMIV010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52497_length_2292_cov_4.288780"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PKV59_07770"
     CDS             complement(<1..1100)
                     /locus_tag="PKV59_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46684.1"
                     /translation="MKNLKLQFILSHTLPVLITIPLVGIALSYAMETHLILKDMKQTI
                     QDQAALITQYSEGNPDVLKEEQAAEQMIQEIRPNLVTGVILFDRAWNVLATTDDDKNE
                     ITQRFPPPMMEMQFGQSGLEPVINISKYNPFKHSEDIIEMMVPVTNSDNQLLGMIRLN
                     FPVTYFEEQLKQTSDRIILILICGVILGVLFGLINAISLEKRLSRTTGAIYDLSLGIR
                     NNPLPEEGPEEMQKLAKAFNSLSQKLDSSEQTRTKLVSYLTHELGRPLGGLSSAVDAL
                     QMGAAQDEKLVQDLTIGMKNEIKRLELLVGDIALLREKTDPINLYVMKPVPLSAWLNE
                     VYSYWAEYARDKEIKLVDEIDQNLPTIKMDENR"
     gene            complement(1097..1795)
                     /locus_tag="PKV59_07775"
     CDS             complement(1097..1795)
                     /locus_tag="PKV59_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQP46685.1"
                     /translation="MAYKILIVDDDVLLRRSLAFNFEKCGYPANTAENAEDALAIINI
                     DPPDLILLDIGLPGMDGLDAISEIRNSKNIPVIFLTARRSGSDQVTGLNAGADDYITK
                     PFDFDVLLAHVKAVLRRRENPVQEPEENILQVGDLKLDFPQHKAEIGGKELELSPKEF
                     ELLTALALNADHIIGTDNLLKRVWGAEYTGDPQVLYVHIRWLRSKIEEDPDHPKRILT
                     VRSVGYKFNSTLSE"
     gene            2067..>2292
                     /locus_tag="PKV59_07780"
     CDS             2067..>2292
                     /locus_tag="PKV59_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46686.1"
                     /translation="MTKIQSIWENFSDDFRSTFQKYKITVILIATVSILYAVFFPKGQ
                     QINSLFGEKIIPFLILFGIGTFLIETLHFKH"
BASE COUNT          612 a          523 c          476 g          681 t
ORIGIN      
        1 cggttttcat ccattttgat cgtcggcaga ttctgatcga tctcatcgac cagtttaatt
       61 tccttatccc gcgcatattc ggcccaatag ctgtagactt cgttcaacca agcggacaat
      121 gggaccggct tcatgacata taggttgatg ggatctgttt tttcccgcaa aagcgcgata
      181 tcacccacca gtaattccaa acgtttaatt tcgtttttca tcccgatggt caaatcctga
      241 accagttttt catcctgtgc cgcacccatc tgaagtgcat ccacggctga tgaaagtccg
      301 cccaacgggc gtcccagttc gtgagtcaaa tacgaaacca attttgtacg agtttgctcg
      361 gaagaatcca atttttggga aagcgaattg aacgctttcg ccaacttttg catctcttcc
      421 ggtccctcct ccggcagcgg gttattccga atgccgagag acaaatcata aatggcgccg
      481 gtcgtccggc tgagccgttt ttcaaggctg atcgcattga tcaaaccgaa aagcacaccc
      541 aaaatgacgc cgcaaatcaa aatcaaaatg attcgatccg aagtctgttt caattgttct
      601 tcgaaataag tcaccggaaa attcaaacgg atcatcccga ggagctgatt atcgctgttg
      661 gttaccggaa ccatcatttc gataatatct tcggaatgtt tgaaaggatt gtatttggaa
      721 atattgatca ccggttcgag accggattgt ccgaattgca tctccatcat cggcggcggg
      781 aatcgttgtg tgatttcgtt cttgtcatcg tctgtggttg ccagtacatt ccaggcgcga
      841 tcgaataaaa tgactcccgt gaccaagttg ggacgaattt cctgaatcat ctgttcggct
      901 gcctgttctt ctttcagaac gtcgggattt ccttcggaat actgtgtgat taacgctgcc
      961 tgatcctgaa tggtttgctt catatctttc aaaatcaggt gagtttccat agcatatgat
     1021 aaagcgatac ccaccaaagg aatcgtaata aggaccggta atgtatggct caggataaat
     1081 tgaagtttaa gatttttcat tcagataaag tcgaattaaa tttgtacccc acgctgcgga
     1141 ccgtcaatat tcgtttggga tgatccggat cttcttcaat tttactgcgc agccagcgaa
     1201 tatggacata aagaacttgg gggtcgccgg tatattccgc cccccagact cgtttcagta
     1261 aattgtccgt gccgataata tgatcggcat tcagcgccaa agcggttaat aactcgaatt
     1321 ctttggggga taactccagt tcttttccgc ctatttcggc tttatgttgg ggaaaatcca
     1381 attttaaatc tcccacctgc aaaatatttt cctccggttc ctgtaccgga ttttcgcggc
     1441 ggcgtaagac cgctttcaca tgggctaaga gaacgtcgaa atcaaagggt ttcgttatat
     1501 aatcatccgc tccggcattc aaaccggtca cttgatcact gccgctgcgc cgggcagtca
     1561 ggaatatcac cggaatgttt tttgaatttc ggatttcgga aatggcatcc agtccgtcca
     1621 ttccggggag cccgatatcc aataagatca aatcgggcgg atcgatatta atgattgcca
     1681 gcgcgtcttc tgcgttttcg gcggtattgg ccggatatcc gcatttctca aagttgaaag
     1741 ccagactgcg tcgcagaagc acatcgtcat caacaatcag gattttatat gccatacaat
     1801 cttcctttta atttaatact tcaaatcata ttgtatacat aaattcagtg attttatcga
     1861 tcttaatgtt gatttaacca cgatggcttg attctctccg gtgaaaacag caccgatttc
     1921 cgatcaagat aatcttgttt ttgataggtt gttaagcatg caaatgctgt caaaacatat
     1981 ccgcaattgt atctcacgaa actttcatta tataactacg tacagaaact aaatctgttt
     2041 gcaaacgaaa gcggaggata aagaagatga caaaaattca atcgatatgg gaaaattttt
     2101 ccgatgattt tcgcagtact tttcaaaaat ataagataac ggttattctg atcgcgacgg
     2161 tttcgattct atatgcggtg tttttcccca agggacaaca gattaattcg ctctttggcg
     2221 aaaaaatcat tcctttcctc atcttattcg gaatcggcac attcttgatc gagacattgc
     2281 atttcaaaca tt
//
LOCUS       DAOMIV010000347         2290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_52572_length_2290_cov_4.359732, whole genome shotgun sequence.
ACCESSION   DAOMIV010000347 DAOMIV010000000
VERSION     DAOMIV010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52572_length_2290_cov_4.359732"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PKV59_07785"
     CDS             complement(<1..295)
                     /locus_tag="PKV59_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46687.1"
                     /translation="MAYFEIGIFQPRNGENIGTLWRSAWQLGAAGIFTIGRKQRKQTS
                     DVLNVPQKIPYRHFDSFEDFLISRPVGTQLIGVEFGGIPLSKFSHPAKAIYLLG"
     gene            complement(297..>2290)
                     /locus_tag="PKV59_07790"
     CDS             complement(297..>2290)
                     /locus_tag="PKV59_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQP46688.1"
                     /translation="IFVLIAWREFLKILPESVGQKLDITEKMWKKAKEIFMVTPAENS
                     REKMKEPEIPIQRFSGESTSPENPSSGNETVTIPGESEYKNDSAKNHFHLSDQKGEFR
                     EKERTENKNQEISTKPVQPVVVAATPESMNKPFTPPLLKKIQVKDLSSHKPLTAEKWR
                     LPATDEILDPLKSQQKITPDDPYVQSQVQKIEHVLEIFDAPGKVVDIQCGPTFTQFGI
                     QPGFLDRQDRKIRVRVKQIEALKKDLEMALSVKQLSIDAPIPGKTYIGLQVQNRHRYP
                     VTLREVIESDGFRKYQNGLGIALGKDIYGETYAADLTRMPHLLIAGATGSGKSVCLNA
                     ILVCLLMHHSPESLKLVLIDPKRVELTGYNGVPHLITPVVTDVEKVSEILQWVLREMD
                     NRNQHFLEAGVRNIQDFNQKFPEQRMPYIVVVIDELANLMMEAATEIESSITRLAQTA
                     RAMGIHLVVATQRPSRDVITGTIKGNLPSRIAFAVATNTDSLVILDRVGAEDLFGKGD
                     MYLMSAEDPNLKRLQCVYVSDDEIRRLVDYWRSNPKISEVSSEEIQPKPELTPITLHQ
                     PSQKFDGVLTQLPLLDENEPAMKRNADPLLDDAIALVRKQGRASANMLVSRLSIGFGR
                     ANKLLVQMEEMGIIGPPNANPSIPREVLDYGENGTPSKE"
BASE COUNT          559 a          544 c          502 g          685 t
ORIGIN      
        1 aaccgagcag ataaattgct ttcgcgggat ggctgaattt tgataatgga attccgccaa
       61 attcaacgcc tatcagctgt gtgccgacgg gacgtgaaat cagaaaatct tcaaaagaat
      121 cgaaatggcg ataagggatt ttttgcggga cattcagcac gtcactggtt tgttttcgct
      181 gttttctgcc gatggtaaat attccggcgg ctcccagttg ccatgcgctt cgccataaag
      241 tgccgatatt ttccccgttc cgcggttgaa aaattccgat ttcaaaatag gccatattat
      301 tctttcgacg gagttccgtt ttctccgtaa tctaaaactt cacgtggaat ggagggattt
      361 gcattcggag gaccgatgat gcccatttct tccatctgta ccaataattt gtttgccctt
      421 ccgaaaccga tactcagccg actgactaac atattggcag aggctcttcc ctgctttctc
      481 accaatgcaa tcgcatcatc aagcaacgga tcagcattcc ttttcatcgc cggttcgttt
      541 tcatccaaaa gcggtaattg tgtcagaaca ccatcgaatt tctgcgatgg ctgatggagg
      601 gttataggtg ttaattcagg tttcggctgt atttcttcgg aggagacttc actgatcttc
      661 ggattcgaac gccaataatc tactaagcgc cggatttcat cgtccgaaac gtatacacat
      721 tgcaagcgtt ttaaattagg atcttcggcg gacatcagat acatatctcc ctttccaaac
      781 agatcttccg ctcccacgcg atccaaaatg accaggctat ccgtgtttgt tgccacggca
      841 aatgcgattc tcgagggcag attgcctttg atggtccccg taattacatc ccgcgatggt
      901 ctttgagtgg ccaccaccag atgaattccc attgcgcgag cagtctgagc aagccgggta
      961 atgctgcttt caatttccgt ggcggcttcc atcatcaggt tcgccaattc atcgattacc
     1021 acgacaatat aaggcattct ttgctcagga aacttttggt tgaaatcctg aatatttcgg
     1081 acgcccgctt ccagaaaatg ttggttgcga ttatccattt cacgcagcac ccattgtaga
     1141 atttcactga ctttttcaac atcggtcaca accggtgtga tcagatgcgg gacaccgttg
     1201 tagcccgtta attccacccg tttggggtcg atcaacacca atttcaagct ctctggagaa
     1261 tgatgcatca ataagcagac taaaattgca ttaagacaaa ctgatttacc ggagccggtc
     1321 gcaccggcaa tcaacaaatg gggcattctg gtcagatcgg ctgcataagt ctctccgtag
     1381 atatctttcc ctaatgcgat tcccaaccca ttttgatatt tcctaaaccc gtcgctttca
     1441 attacttctc gtaaggtaac ggggtaccga tgccgattct gcacctgcag tccgatatag
     1501 gtctttccgg gaatcggagc gtcaatggag agctgtttaa cagacagtgc catctccaag
     1561 tcttttttta gggcttcaat ttgtttgacc cgaacccgga tttttcggtc ttgacgatcc
     1621 agaaaaccgg gctgaatccc gaattgcgta aatgtcggac cgcattggat atcaactacc
     1681 tttcccggcg cgtcgaatat ttccaaaaca tgctctatct tttgaacctg gctttgtaca
     1741 taaggatcgt cgggagtaat tttttgctga gatttaagcg gatccaaaat ctcatccgta
     1801 gcaggcaacc gccatttttc tgccgtcagc ggcttatgag aagaaaggtc ttttacttgg
     1861 attttcttca gcaaaggcgg agtaaacggt ttattcatcg attcgggggt agcggcaaca
     1921 accaccggct gaaccggttt tgtcgaaatt tcctgatttt tattttccgt tctttctttt
     1981 tcgcggaatt cgcctttttg atcggataga tgaaagtgat tcttggcaga atcattttta
     2041 tattccgatt cccccggtat tgtcacggtt tcatttccgg aagaaggatt ttccggtgaa
     2101 gttgattcgc cggaaaatcg ttggataggg atctcgggtt ccttcatttt ttctcggctg
     2161 ttttcagcgg gagtaaccat aaaaatctct ttagcttttt tccacatttt ttcggtgata
     2221 tccagttttt gaccgacgga ttcgggaaga attttgagaa attcgcgcca agcaatcaac
     2281 acaaaaatgg
//
LOCUS       DAOMIV010000348         2286 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_52708_length_2286_cov_4.980726, whole genome shotgun sequence.
ACCESSION   DAOMIV010000348 DAOMIV010000000
VERSION     DAOMIV010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52708_length_2286_cov_4.980726"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="PKV59_07795"
     CDS             <1..943
                     /locus_tag="PKV59_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HQP46689.1"
                     /translation="LVSGYVSMDRIIKINSPARVGYTSLITNQDNAKINYGGCPINIC
                     AALNKLGKRAVPVIRVGDDYEEIGFRDFLIQSGIPLDGVELVPNEKTSCCYLVENPQG
                     EHITLFYTGAMDAKYSRPVPDELIKRTKMALITVGAKPDNEYFLEKVQKYSVPLVFSM
                     KSDPDAFPKELLSEILKESLIIFMNESERKSIEAMFGFGSITDLFSFGKAQILVTTCG
                     ASGSRYYLPDNGNIRSEMIPACRPDQVADTTGSGDAYLAGFMLGYLDQRPISDCCRYG
                     SVLASFIIEKEGCCTNIPTMRQFMDRYNRFIREQEAQ"
     gene            946..1695
                     /locus_tag="PKV59_07800"
     CDS             946..1695
                     /locus_tag="PKV59_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HQP46690.1"
                     /translation="MKTLYMNVDSADIAKNVLFSGDPWRVETVAGYLTDPKRVAFKRE
                     FNTYTGTYKGMPVTVTSTGIGAPSAAIAMEEMYEAGMEVAVRMGTVMSLREGTLGKFI
                     IPYASMRMEKTSATYVPEGYPAVADLDLVQCMNQTVKRMGGEYINGIGCSMDGFYSMM
                     HDSRLSKEMNVDMSVMFEKLKKIRICGVDMESSCILTLGQLMGIKACVLTIVTVLENL
                     KDALEGEERIAAEDLLCRVALEGIYNYRYHV"
     gene            1723..>2286
                     /locus_tag="PKV59_07805"
     CDS             1723..>2286
                     /locus_tag="PKV59_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HQP46691.1"
                     /translation="MTVWDVFRQKRPFIIGMVHCQPLLGTPGFDGSSQNVLYRAIQDA
                     VTLEKAGVDGIMVENMCDDPLGTLLTLEETVGLTAVATMVKNVVGDIPVGIDAAFCDC
                     KASLAIAKTLGCQFVRIPVFVDTVLYSGGIIYPCAHDCMYYRKQIGAEDVMILADVQV
                     KHTHMLMKDISIEQSAKDAVACGADGLI"
BASE COUNT          616 a          442 c          616 g          612 t
ORIGIN      
        1 actggtgagc ggttatgtaa gcatggatcg tatcatcaaa atcaattccc ccgcacgggt
       61 tggttataca tcgctgatca caaaccagga taatgccaaa atcaattacg gcgggtgtcc
      121 gatcaatatt tgtgccgcgc tgaataaact ggggaaacgg gcggtgccgg tgatacgcgt
      181 cggtgatgat tatgaagaaa tcggctttcg ggattttctg atacaatccg gaattccttt
      241 ggatggggtc gagttggttc ccaacgaaaa aacctcctgc tgttatttgg tggaaaatcc
      301 gcagggagaa catatcacct tgttttatac cggcgcgatg gatgcgaaat acagtcgacc
      361 ggttccggat gagcttatca aacgaacaaa aatggcattg attacggtcg gagccaaacc
      421 cgataacgaa tatttccttg aaaaagttca aaaatattcg gtccctttgg tcttcagtat
      481 gaagtcggat ccggatgcct ttccgaaaga attgttaagt gagattctga aagaaagtct
      541 gattattttt atgaatgaat cggaacgaaa aagcatcgaa gcgatgttcg gtttcggctc
      601 gattaccgat ttgttctcct tcggaaaagc gcaaatcttg gtgacaacat gcggagcgtc
      661 gggaagccga tattatctgc cggataacgg taatatccgt tcggagatga ttccggcttg
      721 tcgacccgat caggtggcag atacgaccgg atccggggat gcttatttgg ccggttttat
      781 gttgggctat ctggatcaac gaccgatatc agactgttgc cggtatggaa gtgttttggc
      841 atcttttatt atcgagaagg aagggtgctg taccaatatt ccgacgatgc gacagtttat
      901 ggatcgctat aatcgtttca tccgtgaaca ggaggcgcag taataatgaa aacactttat
      961 atgaacgtag actctgccga cattgcgaag aatgttcttt tctcgggtga tccatggcga
     1021 gtcgaaacgg ttgccggata tttgaccgat cccaagagag ttgcattcaa gcgcgaattc
     1081 aacacttata ccggcacata taagggaatg ccggtaacgg taacttcgac aggcatcggt
     1141 gcaccttccg ctgccatcgc aatggaagag atgtatgaag ccggtatgga agtggcagtg
     1201 cgaatgggga cggttatgtc tttacgggaa ggaacgctcg gcaagttcat tatcccttat
     1261 gcatccatgc gaatggaaaa aaccagcgct acctatgtgc ccgaaggata cccggcggta
     1321 gcggatttgg acttggttca atgtatgaat cagacggtta aacgaatggg gggcgaatat
     1381 attaacggaa tcggttgcag catggacggc ttttattcga tgatgcatga ctcccgtctt
     1441 tcaaaggaaa tgaatgttga catgtccgtc atgttcgaga aacttaaaaa aatccgaatt
     1501 tgcggcgtgg atatggagtc ttcctgcatt ttaaccctcg gacaactgat gggcataaaa
     1561 gcctgtgttc tgaccatagt cactgttttg gagaatctga aagatgcatt ggaaggagaa
     1621 gaacgaatcg ctgcagaaga ccttttatgt cgcgtggcgt tggaaggaat ttataactat
     1681 cgttaccacg tctgatttag cggttacgaa gaagaggaaa atatgacagt ttgggatgta
     1741 tttagacaga aaaggccgtt tattatcggc atggttcatt gccagccgct cttggggacg
     1801 cccggttttg atggaagctc acagaatgtg ctttatcggg cgattcagga tgccgtaacc
     1861 cttgaaaaag cgggtgtgga cggcattatg gttgaaaata tgtgcgatga tccgttggga
     1921 acattgctga ctttggaaga aacggtcggt ttgactgccg tcgcaacgat ggtcaaaaac
     1981 gtggtggggg atatacctgt cgggatcgac gcggctttct gtgattgtaa ggcgtcttta
     2041 gcaattgcca aaacgctcgg ctgtcaattt gtgcgaattc cggtttttgt ggatacggta
     2101 ctatattccg gaggaataat ctatccgtgt gcacatgatt gtatgtatta tcgaaaacaa
     2161 atcggtgcgg aagatgtgat gattcttgcg gatgtccagg taaaacatac tcatatgctg
     2221 atgaaggata tttctattga acaatccgca aaagatgccg tagcctgtgg ggcggatggc
     2281 ttaata
//
LOCUS       DAOMIV010000349         2277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_53008_length_2277_cov_3.815932, whole genome shotgun sequence.
ACCESSION   DAOMIV010000349 DAOMIV010000000
VERSION     DAOMIV010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53008_length_2277_cov_3.815932"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKV59_07810"
     CDS             <1..229
                     /locus_tag="PKV59_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46692.1"
                     /translation="GIFLQIEEEIDLAAERDRLQKELAETDEQIDRLEKLLNSPFSMK
                     APAPVVEKEREKLAKYRETAINLNKQLSGLR"
     gene            complement(295..1452)
                     /locus_tag="PKV59_07815"
     CDS             complement(295..1452)
                     /locus_tag="PKV59_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQP46693.1"
                     /translation="MQYRKFGKTGETVSVLGFGCMRLPVLDGKPEKIDVVKTDEMLNY
                     AFDHGVNYFDTAYTYHRPMNPAVKKGFSEDVLGDCFRGAKREKIFFATKLPTFNIYKK
                     EEFEEALNASLRSLKTDRIDFYLAHSLTKPTWDRMVELGMLEFLQKAKESGKVRHLGF
                     SFHDDLSVFKEIINTFDWEFAQIQYNYLDVNRQAGTEGLHYASSKGLGMTIMEPLRGG
                     KLVSIPSREISDLWKESEHRDWTPAQRGLYFVWNDPAVSVVLSGMGALEQVKENIAAA
                     ENAAPGILSEKELALYSRVRDMYWKRIIAPCTGCEYCMPCPHGVNIPENLNVLNELYM
                     FDNFAESKRAYEFLPKEARAEQCINCGTCLRKCPQKIQIPVHMRALADRMA"
     gene            1616..>2277
                     /locus_tag="PKV59_07820"
     CDS             1616..>2277
                     /locus_tag="PKV59_07820"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQP46694.1"
                     /translation="MKTIFRFLFIFALLISIGSCGTINQMIHPPTATPTATLTATITP
                     TPLPTLTPTPPPAVAIVNGIYIWQEDFDMTVANLRRAYSDTQSMQPSEETLKQEALDT
                     LIDETLFQAAAVKDGIIISDEELDTRIQSLIEKAGGSDAFRQWLSENHYSESGFRRAL
                     TREISAAKIREKLFSEKLTGIEQIHAYQIFTATHAEANTIKTKLDLGLNFIDLAKTND
                     PIT"
BASE COUNT          667 a          553 c          425 g          632 t
ORIGIN      
        1 cggaatcttt ctacaaatcg aagaagaaat cgatttggct gccgaacggg atcgtctgca
       61 aaaagaatta gcagagaccg atgaacagat cgatcggctt gagaaacttt taaattctcc
      121 gttttccatg aaagctccgg ctcctgtcgt tgaaaaagag cgtgaaaagc ttgcaaaata
      181 ccgggagaca gctattaatc ttaataagca actgagcggt ttgcgctaaa taatcttctc
      241 tttcggaaac aaaaagtccc tttttatcat ttgaaaggga cttttttgtc gatttcaagc
      301 catgcgatcg gccaaggcac gcatatgaac cggaatttga atcttttgcg gacattttcg
      361 caaacatgtt ccgcaattaa tgcactgttc ggctctggct tctttgggta aaaattcata
      421 agcacgcttg ctttccgcaa agttatcaaa catgtaaagt tcattaagaa cattgagatt
      481 ttccgggata ttgactccgt gcgggcaggg catacaatat tcacagcccg tacaaggtgc
      541 gataatccgt ttccaataca tgtcccgaac ccgagaataa agagccagct ctttttcgga
      601 caaaatgccc ggtgcggcgt tttccgcggc agcgatgttt tctttgacct gctccaacgc
      661 tcccattccg ctcaaaacca ccgagactgc cggatcattc catacaaaat acaaacctcg
      721 ctgtgccggt gtccaatccc gatgttcgga ttccttccaa aggtcactga tttctcttga
      781 gggaatggat accaactttc cgccccgtaa aggttccata atcgtcattc ccaatccttt
      841 ggaagatgcg taatgcaatc cttcggtacc ggcttgcctg ttgacgtcta aatagttata
      901 ttgaatttga gcgaactccc aatcaaaagt attaataatt tccttaaata cggataaatc
      961 atcatgaaac gagaaaccga ggtgtcggac ttttccggat tctttcgcct tttgtaaaaa
     1021 ttccaacatc cccaattcaa ccatgcgatc ccaagtcggc ttcgttaaac tatgcgccag
     1081 gtaaaaatcg attcgatctg ttttcaagga acgcagagag gcattcaaag cttcttcaaa
     1141 ttcttctttt ttatagatat taaaggttgg caatttagtg gcaaagaata ttttttcccg
     1201 ctttgctcct cgaaaacaat caccgagcac atcttcgcta aacccttttt tgactgccgg
     1261 attcatcggg cgatgatagg tataagcggt atcaaaataa tttacaccgt gatcaaaagc
     1321 ataattcagc atctcatcgg ttttaaccac atcaatcttt tcgggtttcc catccaacac
     1381 cggcaaacgc atacaaccaa accccagtac cgaaacggtc tcacccgttt tcccaaattt
     1441 tctgtattgc atttttcgac ctcccccgga aataaactcc ggatcaaatg ttatcgataa
     1501 agattaagaa tattgtattt caatcttccg gatttcggaa ataaaaaaat gcactcatcg
     1561 gaatattttt cttttcatcc tctatatttc aaacacttag tataataatt tcctgatgaa
     1621 gaccatattc cgatttttat ttattttcgc cctgctgatt tcgatcggtt cgtgcggcac
     1681 aatcaatcaa atgattcatc cgcctacagc cacgccgaca gcgacattga ccgccaccat
     1741 taccccgacc cccttaccga ccctcacacc gacaccgccg ccggcagtgg caattgtaaa
     1801 cggtatttat atttggcagg aagattttga tatgaccgtt gccaaccttc gccgggcata
     1861 ttcggataca caatcgatgc aaccttccga agaaacttta aaacaagaag ctttggatac
     1921 cttaatcgat gaaaccttat tccaagctgc cgcggtaaaa gacgggatca tcatatcgga
     1981 tgaagaattg gatacccgaa ttcaatctct cattgaaaag gcgggaggat cggatgcttt
     2041 tcgacaatgg ttatccgaaa accattatag cgaaagcggc tttcggcggg cattaacccg
     2101 tgaaatttct gccgccaaaa ttcgagaaaa actcttttcg gaaaaattaa cgggaatcga
     2161 acaaattcat gcctatcaga tctttaccgc tacccatgcc gaagccaata ccattaaaac
     2221 aaaactggat ttggggctga attttatcga tcttgccaaa accaacgatc ccattac
//
LOCUS       DAOMIV010000350         2273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_53165_length_2273_cov_3.113165, whole genome shotgun sequence.
ACCESSION   DAOMIV010000350 DAOMIV010000000
VERSION     DAOMIV010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53165_length_2273_cov_3.113165"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKV59_07825"
     CDS             complement(<1..184)
                     /locus_tag="PKV59_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46695.1"
                     /translation="MKKQFTLNGDWTMRRLDRSETIPATIPGSVYSTLLAAGKMDDPF
                     YRDNEMNALPLMEYDYS"
     gene            complement(209..1216)
                     /locus_tag="PKV59_07830"
     CDS             complement(209..1216)
                     /locus_tag="PKV59_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP46696.1"
                     /translation="MTSIKDISQACGYSPSTVSKSLNYATDVGKETAEKIRNVAEQMG
                     YFPNSIARALKTNRTFNIGVLFVDATASGLTHEFFSSILNSFKVEADRRGYVITFISN
                     QINRNRVSLLEHCRHRNYDGVMIACVNFKNPEVAELVSSEIPTLSIDYEFPTCTSILS
                     DNLAGMKELTDYVIQKGHRKLAIIHGEGTEVTHIRLDSFFRTLNEKQIRIPKYFIKPA
                     LYHEPLHSQIATRELLSLPERPTCIFYPDDYSLLGGLDELEQHHLKVPEDMSIVGYDG
                     IFLSQILRPRITTMHQDTETIGERAAEELIEQIENPRTYKRHRVVVKQDLLEGETVQQ
                     L"
     gene            complement(1433..>2273)
                     /locus_tag="PKV59_07835"
     CDS             complement(1433..>2273)
                     /locus_tag="PKV59_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP46697.1"
                     /translation="PDIFMSKEILIKAFEKGKHVLCEKPMATTLEDCQKMVEAAERNG
                     KFLMIGQNQRLAKAHVKARKLIESGEIGKILTFRTTFGHPGPENWTGQKNSWFFDKTK
                     AAFGAMADLGVHKTDLIHFLTGDVITEVTAQLGTLDKKYPDGTPISVDDNAYCIYQTR
                     GGIIGTLHVSWTFYGDEDNSTVLYGTDGVIRCYDDPKYSLIVEKRDGGRAYYILDQMT
                     SNKEQTSGARTNTGVIDTFVNCILTNTPPAISGREVMKAMKVIFAAEESAQNGKKVIV
                     VQD"
BASE COUNT          593 a          502 c          469 g          709 t
ORIGIN      
        1 acgaataatc atattccatt aaaggcaagg cattcatctc attgtcccga taaaaagggt
       61 cgtccatttt accggcggct aaaagagtac tgtaaaccga tccgggaatt gttgccggta
      121 ttgtttccga tcggtcaagc cgtctcattg tccaatcgcc gtttaatgtg aattgttttt
      181 tcataaatat cgactccaaa aagctttttt aaagttgttg aacggtctcc ccttccaata
      241 aatcttgttt cactaccaca cgatgtcttt tataagttct cggattttcg atttgttcga
      301 ttaattcttc ggccgctctt tccccgatcg tttccgtatc ttggtgcatt gttgtgatgc
      361 ggggacgtaa aatttgagaa aggaaaatgc cgtcgtatcc gacgatgctc atatcttcgg
      421 gaaccttcag atggtgttgt tccagttcat ccaaaccacc taaaagtgag taatcatccg
      481 ggtagaaaat gcaggtcggt cgttcgggca gggataataa ttcgcgggtg gcaatctgac
      541 tgtgaagcgg ttcgtgatat aaagcgggtt taataaaata cttcggaatc ctgatttgct
      601 tttcattaag agtccggaaa aagctgtcta atcggatatg agtcacttct gtcccttcgc
      661 cgtggatgat tgccagtttg cgatgccctt tttgaatcac ataatcggtc aattctttca
      721 tccccgccag attatcggaa agaatggatg tgcaggtcgg aaattcgtaa tcaatggaaa
      781 gagtgggaat ttcactgctg accaattccg ccacttccgg atttttgaag ttgacacatg
      841 cgatcatgac accgtcataa tttcgatgcc tgcaatgttc cagcaaactt acgcgattgc
      901 ggttaatttg attgctgata aaggtgatta cgtatccgcg gcgatccgct tcgactttaa
      961 agctgtttag aatggaggag aaaaattcat gcgttaaacc gctggcggtt gcgtcgacaa
     1021 aaaggacgcc gatattaaac gtccgattgg tctttaaggc tcttgctatt gagttgggga
     1081 aatatcccat ttgttctgcg acatttcgaa ttttttcggc ggtttccttc ccaacatcgg
     1141 tcgcataatt taatgatttg ctgacggttg aaggtgaata tccacacgct tgagaaatgt
     1201 ctttaataga cgtcatccga aaatctttat ccttatttga tgattttcag aaccaattgt
     1261 ctgaacgaaa tcgttttcgt aaaaccgatt atacgacttc attttgataa aaaaagtaat
     1321 attaaatcat gaattttgta tgataaatat cctattaaag atatataaaa aaagagtatc
     1381 gcttggaccg gcgatactct ttttcttctc gaaagggaat tattttcctc cgtcagtcct
     1441 gaacgacaat cacctttttc ccattttgag ccgattcttc ggctgcaaag atgactttca
     1501 tcgccttcat tacttctcgt ccggaaatag cagggggtgt attggtcaaa atgcaattaa
     1561 caaaggtatc gatgacgccg gtatttgtcc ttgcgccgga agtttgttct ttattggacg
     1621 tcatctggtc caaaatgtaa taggcacgtc cgccgtctct tttttctaca atcagagaat
     1681 acttcggatc atcataacaa cggattaccc catccgtacc gtaaagcacc gtcgagttat
     1741 cttcatctcc gtaaaaagtc caactgacat gaagcgttcc gataatgcct cctctggttt
     1801 gatagatgca ataagcgtta tcatctacgg aaatcggagt cccgtccgga tattttttat
     1861 ccaacgtccc taactgcgcg gtgacttcgg tgatcacatc tcccgtcagg aagtggatca
     1921 gatccgtttt atggactccc aaatcagcca tggctccgaa agccgctttt gttttatcaa
     1981 aaaaccagga atttttctga ccggtccaat tttccgggcc gggatgcccg aaagtggtac
     2041 gaaaagtcaa aattttaccg atttctcccg actcaatcag cttccgggct ttgacatgtg
     2101 ctttcgccag gcgttgattt tgcccgatca ttaagaattt cccgttccgt tccgccgctt
     2161 caaccatttt ctggcaatcc tccaaagttg tggccatcgg tttttcacag agaacatgct
     2221 ttcctttttc aaaagccttt attaatatct ccttactcat aaaaatgtcg gga
//
LOCUS       DAOMIV010000351         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_53445_length_2265_cov_2.577828, whole genome shotgun sequence.
ACCESSION   DAOMIV010000351 DAOMIV010000000
VERSION     DAOMIV010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53445_length_2265_cov_2.577828"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..668)
                     /locus_tag="PKV59_07840"
     CDS             complement(93..668)
                     /locus_tag="PKV59_07840"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014483815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HQP46698.1"
                     /translation="MKLLEDRILRDGVVKGGNILKVDSFLNHQIDVRLYDEIGKELYR
                     LFKDEPVTKVLTIESSGIGLAVAAAREFDVPALFAKKNRTKNIDGEVYSSAVESFTTG
                     ITYTIIVSKHFLGPQDHVLIVDDFLANGNALLGLIEIVEQSHAKLVGCGIAIEKGFQS
                     GGKMIREKGIRVESLAIIDKMDENGIVFRPQ"
     gene            complement(778..>2265)
                     /locus_tag="PKV59_07845"
     CDS             complement(778..>2265)
                     /locus_tag="PKV59_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HQP46699.1"
                     /translation="GFTGEPQKNGFFEHHYTNENEVIRTPNWAKGIIYYQIFPDRFAR
                     GIDQKSRHPYVDWNSRPALDNYFGGDLPGIIQKLDYLANLGIVCIYLNPIFHADCNHK
                     YATIDYFSVDPDFGTNDDLIRLVKKAHDLGIRVILDGVFNHCGNNFPPFVDFRMNGKA
                     SPYADWFLPTDFNAEGTLSRYEGFGDLSLMPKLRTGTPAVREMILKVMTYWVETAGID
                     GWRMDVADEVETQSWQVICAAFKHLYPDKLLLAETWTDANRLISYGDRFDCAMNYAFR
                     DALSDFFAKRRIDSFGFDHRINHLLSKYNEEVTPLMYNLIDSHDTPRFLTESGEDKRR
                     LKLAVVFQMTCLGSPAIYYGDEIGMEGNDDPDCRGGMKWDPKDQDHDLLSFYKTLISL
                     RKREKALKSGSYVSILCEPNGIYGFQRKHENETVYVIINNSDKEIAVELPVNIPFHPF
                     RDLFNHELFESIPLSEDDKIWNNDIAVHNGKIRMKLPAMGFRILK"
BASE COUNT          651 a          525 c          444 g          645 t
ORIGIN      
        1 ggcgttttcg agagaaattt gaacaacgga tatttttaac tctgttgccc aaaacttgaa
       61 ccatcgattg aaaaccgtct tctcagaata aattattgcg gacggaagac aatcccgttt
      121 tcatccattt tatcaataat ggctaaagat tcgactcgga ttcctttttc ccggatcatt
      181 tttccgccgc tttggaaacc tttttcgata gcaatgccac aaccgaccaa tttggcatgg
      241 gattgttcaa caatttctat gaggcctaaa agggcattgc cgttagccag aaaatcatcc
      301 acgatcagca cgtggtcctg cggtccgaga aaatgtttgg agacgataat cgtataggta
      361 attccggttg taaacgattc gaccgccgag gaatagactt ctccgtcgat gttttttgtt
      421 cgattctttt tcgcaaatag tgcgggaacg tcaaattccc gggcagctgc gacagccaga
      481 ccgattccgg aactctcgat agtcaggact tttgtgaccg gttcatcctt aaaaagtcga
      541 taaagttctt ttccgatttc atcataaagc cggacatcaa tctgatggtt cagaaagctg
      601 tcgactttta agatattgcc gccctttacg acgccgtctc ttaagattcg atcttccaat
      661 aatttcaaga catacctccc ctatctgaat aacaagttaa atttaataca cggatgaatg
      721 cctattataa aagcatttgt ctgaatcact tcggaaggaa aaggaaaatg ctgattctta
      781 ttttaaaatt ctgaaaccca tagccggcag tttcatccga attttaccgt tatgaacggc
      841 tatatcattg ttccaaattt tatcatcttc ggaaagaggg atcgattcga acaattcatg
      901 gttgaataaa tcacggaagg gatgaaaagg aatattaacg ggcagttcga ctgctatttc
      961 cttatccgaa ttattgatta ttacataaac ggtttcattc tcgtgtttcc gttgaaatcc
     1021 gtatataccg ttcggctcgc aaagaataga aacgtagctg cctgatttca acgctttttc
     1081 ccgctttcgt aaagaaatca acgttttata aaaagacaat aaatcatgat cctgatcttt
     1141 tggatcccat ttcattccgc ctctgcaatc cggatcatcg ttcccttcca tcccgatttc
     1201 gtcaccgtaa taaatcgccg gtgatccgag gcatgtcatt tgaaagacaa ccgcaagttt
     1261 caaacgacgt ttgtcttctc ccgactcggt caaaaaacgg ggtgtgtcat gactgtcaat
     1321 caggttatac atcagcggtg taacttcctc gttatattta gaaaggaggt gattgattcg
     1381 atgatcaaat ccgaaagaat cgattctgcg cttggcaaaa aaatccgata aagcatcccg
     1441 aaaagcatag ttcattgcgc aatcgaatcg gtcaccataa ctgatcaagc gattcgcatc
     1501 tgtccaagtc tcagccaata aaagtttatc gggatagaga tgtttaaatg ccgcacatat
     1561 tacctgccag gattgcgttt cgacttcatc cgcaacatcc atccgccatc catctatgcc
     1621 ggcagtttcg acccaatacg tcattacttt caagatcatt tctcggacag ccggcgtgcc
     1681 ggtacgtaat ttaggcataa gggataaatc gccgaaccct tcgtaacggc tcaaagttcc
     1741 ttccgcatta aaatccgtcg gcagaaacca atccgcatac ggagaagctt tcccgttcat
     1801 tcggaaatcg acaaaaggcg gaaaattatt tccgcaatga ttgaacactc catccaaaat
     1861 cacccgaatc ccaagatcat gcgctttctt aaccagacgg attaaatcat cattcgtccc
     1921 gaaatcggga tcgacggaga agtaatcaat cgtcgcatac ttatggttac aatccgcatg
     1981 aaaaatcggg ttcaaatata tgcatacaat tccgagattt gccagataat ccaatttttg
     2041 aatgattccc ggcaaatctc cgccaaagta attatccaaa gcaggtcggc tgttccaatc
     2101 tacataagga tggcgggatt tttggtcgat cccgcgggcg aaccggtcgg gaaaaatctg
     2161 gtaatagatg attccctttg cccaattcgg cgttcggatc acttcatttt cattcgtata
     2221 gtgatgctca aagaagccgt ttttttgcgg ttcaccggta aaccc
//
LOCUS       DAOMIV010000352         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_53953_length_2249_cov_4.686418, whole genome shotgun sequence.
ACCESSION   DAOMIV010000352 DAOMIV010000000
VERSION     DAOMIV010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53953_length_2249_cov_4.686418"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..690)
                     /locus_tag="PKV59_07850"
     CDS             complement(25..690)
                     /locus_tag="PKV59_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46700.1"
                     /translation="MIRKNFFCYLLIPLSVTACVQHLPREIVTSTVPNETSVAQTTAD
                     TAETPVAAEEYALPEGTLVWTDASVTRTLPAGATYADPWDYCQAVGTIDTPGPEYTGT
                     NPPEVIKKEVLRALAISESDGTAHNVVWRCVDGQVFGCDSSMYSTCLTPMNLSTTPPE
                     AAVNECAKAEMDNITLPGAVVGNDTPYEWTCQGGKPVITGQGVTIDEQGYNALIWYPV
                     PRQ"
     gene            930..1700
                     /gene="larB"
                     /locus_tag="PKV59_07855"
     CDS             930..1700
                     /gene="larB"
                     /locus_tag="PKV59_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HQP46701.1"
                     /translation="MNDQQIRQIMEAVASGAISVSEGIKALGEFPQESVGNFAVIDHQ
                     REIRTGFPEVIFGPGKTEEQIVEIFERLHDKNPNVLASRISPEVYEHIRERLPYATYD
                     PNARILYTKEKQELLEGITVVTAGTGDIPVAEEAALTAELMGNRVERIYDVGVSGIHR
                     LFNRLPDLRKAKVIIVVAGMEGALASVVGGLVQCPVIAVPTSVGYGSNFHGLAALLSM
                     LNSCANGVGVVNIDNGYGAACLASKINHLRVQKDLQDD"
     gene            1693..>2249
                     /gene="larE"
                     /locus_tag="PKV59_07860"
     CDS             1693..>2249
                     /gene="larE"
                     /locus_tag="PKV59_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HQP46702.1"
                     /translation="MTEKSELASEIQSKLDKLQEILREMGSVIVAFSGGVDSSLLAYL
                     AHETLGDRSLAITVSSELDPPDTIDTAEAFAKKHQIPHEIVKQNLLKVESIASNPSNR
                     CYYCKRQILTEMIRIAKERNFAFVIEGQNIDDLGDYRPGREAIKECGVRSPFVEAGMT
                     KLEIREISKTFQLNTWDQPSTPCLAT"
BASE COUNT          605 a          528 c          548 g          568 t
ORIGIN      
        1 attcatagaa aactccgcta tagtttattg tctcggtacc ggataccaaa tcagagcgtt
       61 atatccctgt tcatctatgg tcaccccttg accggtaata acgggctttc cgccttggca
      121 ggtccattcg taaggagtgt cgttcccgac aaccgcaccc ggtaatgtta tattatccat
      181 ttcagccttg gcgcattcgt tgacagccgc ttccggcggg gtcgtgctta ggttcatcgg
      241 agtcaggcaa gtcgagtaca tcgaactgtc gcagccaaac acttgcccat ccacacaacg
      301 ccataccaca ttatgagccg tgccatccga ttcggaaata gccagggcgc gtaatacttc
      361 tttttttatg acttccgggg gattcgttcc cgtgtattcg ggtccgggtg tatcaatcgt
      421 tccaaccgcc tggcaataat cccaaggatc ggcataagtt gctcccgccg gcaacgtgcg
      481 ggtcaccgat gcatccgtcc acacgagagt accttccgga agagcatatt cctcagccgc
      541 aaccggagtt tctgcggtat ccgctgtggt ttgggcaacg gaagtctcgt ttggcaccgt
      601 agaggtaaca atttcccgtg gcaggtgctg aacacaggct gtcacactaa gagggatcag
      661 aaggtaacaa aaaaaatttt tccgaatcat cgctgcgcct tattcaataa aatatatcta
      721 cgcttttagt atatcgcaac tataaaataa gatggactat ccgcttcacc cgcgagatta
      781 ccaaaataaa atatcccaac tcttttcgat caaaaaataa tgatctgaat cgacttttgc
      841 ggaaatcttt tgaatcccat atatcccgcc tgacggatta tcgaatagaa ttaaacggta
      901 gaaactaaat gtcggctcgg agaattttta tgaatgatca acaaattcga cagattatgg
      961 aagccgttgc aagcggtgcg atatctgttt cggaagggat aaaagcgttg ggcgaatttc
     1021 cgcaggaatc ggtagggaat tttgccgtaa tcgatcatca gcgggaaatc cgcaccggat
     1081 ttccggaggt aatcttcgga ccggggaaaa cggaagaaca gatcgttgaa atatttgaaa
     1141 gattacatga taaaaatccg aatgtattgg cttcgcggat cagtccggaa gtttatgaac
     1201 atattcgaga acgcctgcca tatgccacct atgatcccaa tgcccgaatt ctatatacga
     1261 aggagaagca agaattattg gaagggatca cggtagttac cgccggcacc ggtgatattc
     1321 cggttgccga agaagccgct ctgacagcgg aactgatggg aaaccgagtc gagcgcatat
     1381 acgatgtcgg cgtttcaggt attcatcgtc tctttaaccg tctgccggat ctccgaaaag
     1441 caaaagtcat catcgttgtc gccggaatgg aaggagcgct cgccagcgtc gtcggcgggt
     1501 tggttcaatg tccggtgatt gccgttccta ccagcgtcgg ctacggttcc aattttcacg
     1561 gcttagctgc tttgctttcg atgttaaaca gctgtgccaa cggtgtcggg gttgtgaata
     1621 tcgataacgg atacggtgcg gcttgtctgg cttccaaaat caatcatctc agagttcaaa
     1681 aggacttgca agatgactga gaaatcggaa ttggcttctg aaatacagtc aaaattggat
     1741 aaattgcaag aaatcctccg ggaaatggga tcggttattg ttgctttttc agggggtgtg
     1801 gacagcagcc ttttggctta tctcgcccat gaaaccttgg gagatcgaag tctggcgatc
     1861 accgtctcat cggaattgga tccgccggac accatcgata ctgccgaagc gtttgcgaaa
     1921 aaacatcaaa tccctcacga aatcgttaaa cagaatctgt tgaaagtcga atcgatcgcg
     1981 tccaatccgt cgaatcgctg ctattactgc aagcgccaaa ttcttacgga aatgatccgt
     2041 atcgcaaagg aacggaattt tgcatttgta atcgaaggac agaacatcga tgatctgggt
     2101 gattaccgtc cgggtcgaga agcgataaaa gaatgcggcg ttcgcagtcc gtttgtcgaa
     2161 gccgggatga ctaaattgga gattcgggag atatcaaaaa cttttcaact gaacacatgg
     2221 gatcaacctt ccactccctg tctggcaac
//
LOCUS       DAOMIV010000353         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_54118_length_2245_cov_3.880365, whole genome shotgun sequence.
ACCESSION   DAOMIV010000353 DAOMIV010000000
VERSION     DAOMIV010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54118_length_2245_cov_3.880365"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..583)
                     /locus_tag="PKV59_07865"
     CDS             complement(17..583)
                     /locus_tag="PKV59_07865"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQP46703.1"
                     /translation="MAEQPINQKLSRFQEIRSNKKIRIGLIIFVLSALTILFIKSDFE
                     TIKNFIRDHQILGIAVSLLLYILFGVFFIPTEPLTIFLGALMGVLQSVIICIVGNTLA
                     SLIEYYIGSLIGDISDFEKQKENLPLKLGKMPVKSLPFQFGVRAIPGIGSKFVSVASG
                     IYKVSIPYFLAASVISNFINSACDTHKF"
     gene            764..1750
                     /gene="fni"
                     /locus_tag="PKV59_07870"
     CDS             764..1750
                     /gene="fni"
                     /locus_tag="PKV59_07870"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HQP46704.1"
                     /translation="MSIRSRKENHLNACLNSDVQSEISNGFENYRLINESCPEEDFSA
                     LNFETDFLNHKISFPLMISSMTGGTEQSEQINRNLARAAQATNLPFALGSQRIYLVNQ
                     KLETLKYIREEAPDIPILANIGAVQLNYGWTKNELLKLVDSVNANALILHFNPLQEVF
                     QPEGNTDFRGLIDKIKELCADFPVPIVAKEVGFGISVSTAAKLADAGIRWIDVAGAGG
                     TSWAKIEALSAGQKIPAETIAPFGGWGIPTAVCIDQIHQKMPEINLIASGGIRNGIEM
                     RKACLLGAKLCGIAIPLLRPALENAEAVITVLERYIFQYRAAVFTSSAVEKI"
     gene            1804..>2245
                     /locus_tag="PKV59_07875"
     CDS             1804..>2245
                     /locus_tag="PKV59_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQP46705.1"
                     /translation="MSDFQKEIIRQINETLYRQIAKEEIEKKDQTLSDMLFYALGMDS
                     DGTVHAGGKRIRPLICCLTCGAISGSHEPALLPGTVLEMFHNFTLIHDDIEDSGETRH
                     GQPAVWKKWGSALSLNAGDMLFALTYKTLHEISGRSKTGSEISVF"
BASE COUNT          694 a          399 c          517 g          635 t
ORIGIN      
        1 tattccgggg atcgggtcaa aatttgtgag tgtcgcaagc ggaatttata aaattcgata
       61 tgacactggc cgcgaggaag taagggatgg aaactttata aatcccgctt gcgacactca
      121 caaattttga tccgatcccc ggaatagccc tgactccgaa ctgaaaaggt aaagatttta
      181 caggcatttt ccccagtttt aagggtaagt tttctttttg cttttcaaaa tcggagatat
      241 ctccgatcaa acttccgata tagtattcaa tcaaagatgc aagagtattt cccacgatgc
      301 agataatgac ggattgtaag acacccatta atgcgccgag aaaaattgtt aaaggttcgg
      361 ttggaataaa aaataccccg aatagaatat aaagcagtag actgacagca ataccgagga
      421 tttggtgatc ccggataaaa ttttttattg tttcaaagtc tgatttgata aatagaattg
      481 taagagccga cagtacaaaa ataattaatc cgattctgat ttttttattg cttctgattt
      541 cttggaatcg ggatagtttc tggttgattg gttgttctgc catgagggag cgatttcctg
      601 atttatataa gatattgatt tatcttattc tatcattgaa tcaatgaaaa aaattcatag
      661 ttataattat agaactatat aagatataat atattttaat tcaatccggg caatccgatg
      721 aaggcggatt catggtaaac tgatgggaga aaaaaggaag agaatgtcaa tccgttcaag
      781 aaaagaaaat catttgaatg catgcctgaa ctccgatgtt caatcggaga tttccaacgg
      841 ctttgaaaat taccggctga ttaacgaatc ttgtccggaa gaagatttta gcgctttgaa
      901 ttttgaaaca gattttttaa accataaaat ttcatttcct ttaatgattt cctctatgac
      961 tggtggaacg gaacaaagtg agcaaatcaa tcggaatctg gcacgggcag cgcaagcgac
     1021 gaatcttcct tttgcgttgg gctcacagcg catctacctg gtaaatcaaa aattggaaac
     1081 attgaaatat attcgggaag aagcgccaga tatcccgatt ttggcaaata tcggtgcggt
     1141 tcaattgaat tacgggtgga caaaaaacga gttattaaaa ttggttgatt cggtcaacgc
     1201 aaatgcgttg atcctgcact ttaatcctct gcaagaagtt tttcagcctg aaggaaatac
     1261 cgacttcagg ggattgatcg ataaaattaa agaactttgc gccgatttcc ccgttccgat
     1321 tgtggcgaaa gaagtcggat tcggaatatc tgtttcgacg gcggcaaaac tggctgatgc
     1381 aggaatccga tggatcgatg tagcgggtgc agggggaact tcgtgggcaa aaatcgaagc
     1441 tttgagtgcc ggtcagaaaa ttccggctga aacaattgcc cctttcggtg gatgggggat
     1501 tccgacggct gtttgtatcg atcaaattca tcaaaagatg cccgaaatca atttgattgc
     1561 atccggtgga attcgtaatg gcattgaaat gcgaaaagct tgcttgctcg gcgcaaaact
     1621 ttgcggaata gcgattccgt tattgagacc cgcgttggaa aacgcggaag cggtgataac
     1681 tgttttggaa cgatatatct ttcaatatcg tgcggctgtt tttacatcat ccgccgttga
     1741 aaaaatatag aatttttcaa tagagaaaaa attggacccg atatcaatca gggagaaaca
     1801 attttgagcg atttccaaaa agaaataatc cgacaaataa atgaaacact gtatcgacag
     1861 attgcgaaag aagaaatcga aaaaaaagat cagacgcttt ccgatatgct tttttatgcg
     1921 ctcggaatgg attcggatgg aacagtacat gccggcggga aacgaattcg gccgttgatc
     1981 tgttgcttaa cttgcggcgc gatttccggt agtcatgaac cggctttgtt gcccgggaca
     2041 gttctcgaaa tgtttcataa tttcacgctt atccatgatg atattgaaga ttcgggagaa
     2101 accagacatg gccaaccggc tgtgtggaaa aaatggggca gcgcgttgag tctgaatgcc
     2161 ggcgatatgc tgttcgctct gacgtataag acattgcatg aaattagtgg tcgttcaaaa
     2221 accggttcgg aaatttctgt gtttg
//
LOCUS       DAOMIV010000354         2228 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_54775_length_2228_cov_2.624942, whole genome shotgun sequence.
ACCESSION   DAOMIV010000354 DAOMIV010000000
VERSION     DAOMIV010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54775_length_2228_cov_2.624942"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2014)
                     /locus_tag="PKV59_07880"
     CDS             complement(<1..2014)
                     /locus_tag="PKV59_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46706.1"
                     /translation="MRKAEKFIIGKYFPLTLLLIMGITWFIQVPRYGFYLDDWIYLTA
                     YDQGGIHGLINYSLIDSRPGLLPWVIGLGFKIVGNNRLGWQIYSLFWRYLGGVFNWLL
                     IRKLWPSRKDIAAYSAILFMIFPFFKHQAFSIAYNQNWVQYALLILSFLLTIMAIRTD
                     SKRKKIIFFLLSYLLSAAQLMMTEYLLSLEAIRVVLIWFALRDRPYSKRKRFSQTIKI
                     VLPYWGILFAYIVWRFAIMPQMIHDRNEINLFSEHPGFIDLFFYLVQMLIQYMTESVW
                     GIWYRSLDPANIDLSIPSIRAALIIGTFVSAILIIAFTKIRKTDGFFDENQEGKEILL
                     VGILATLLGYLPGIAMDKSPSTQFLYHDRFLIPSFWGISLALTAFIAVYLKGFRKKII
                     FLSLLCGICVFFQIKNSIYYRNAWESQQNFQWQMKWRAPDLKANTAVLGDAIIASFMG
                     GWADGAMVFEMYGKNDGYNPTPYWYLVVGEGDYKDEFDQKLPITYVNKIYDFKAQFGD
                     FLVLTKPEWDRCLWVLDEADLKNPYIQPVTKNLIQYQNKSRILYDSDYKMPEGVFGSD
                     YRHDWCYYYENAAREVDLMNYDKALELYKEAVAANMDMKNPVELTPFIRSAAMTGQWD
                     FAAELTKKGSAQPHITWEYYKNLWDLLFRETPDSADRANAYKSIQEY"
BASE COUNT          721 a          476 c          386 g          645 t
ORIGIN      
        1 tatattcctg aatgctttta taagcatttg cccggtcggc tgagtcgggc gtttcgcgaa
       61 acagaagatc ccaaagattt ttatagtatt cccaggttat atgaggttga gcgcttcctt
      121 ttttggttaa ttcggcagcg aaatcccatt gcccggtcat cgcagcggac cggataaaag
      181 gggtcagttc caccggattc ttcatatcca tattcgctgc aaccgcctct ttataaagct
      241 ccaacgcttt gtcgtaattc atcaggtcaa cttctctggc tgcattttca tagtaatagc
      301 accagtcatg gcgataatcg gaaccgaaaa cgccttccgg cattttatag tccgagtcgt
      361 acaaaattcg acttttattt tggtattgaa taagattttt tgtaaccggt tgaatatatg
      421 gattctttag atccgcttca tccaaaaccc ataggcaacg atcccattcc ggcttcgtca
      481 ataccaaaaa gtctccaaat tgagctttga agtcataaat cttattgaca taggtgatcg
      541 gaagtttctg atcgaattca tccttgtaat cgccttctcc taccaccaaa taccaataag
      601 gtgtcggatt ataaccgtcg ttttttccgt acatttcaaa aaccattgca ccgtcagccc
      661 aaccacccat aaatgaagcg ataatcgcat ctcccaaaac cgctgtattc gctttcagat
      721 ccggtgctcg ccatttcatt tgccactgga agttttgttg ggattcccaa gcattccgat
      781 agtaaattga attttttatc tgaaaaaata cacaaatgcc gcaaaggaga gacagaaaaa
      841 taattttttt tcgaaatcct ttgaggtaaa cagcaataaa agccgttaga gccaacgaaa
      901 ttccccaaaa actcgggatc aaaaaacgat catgataaag aaattgcgtg gaagggcttt
      961 tatccattgc tatccccggc aaatatccca acagagtcgc aagaatcccc accaggagaa
     1021 tttctttccc ctcttggttc tcatcgaaaa atccgtctgt ttttcgaatc tttgtaaatg
     1081 cgataattaa aatcgctgat acaaacgttc cgatgattaa tgctgctctt atactcggaa
     1141 tactcaaatc gatatttgcc gggtctaaac tccgatacca aattccccat accgattcag
     1201 tcatatattg gataagcatt tgtaccaaat agaaaaatag atcaatgaaa ccgggatgtt
     1261 cgctgaacaa attaatctca ttccgatcat gaatcatctg aggcattatg gcaaaacgcc
     1321 aaacaatata tgcaaaaaga attccccaat agggaagaac gattttaatc gtttgagaaa
     1381 atcgctttcg ctttgagtat ggtcgatcgc gtagcgcaaa ccaaatcagc accacccgaa
     1441 tagcttccaa cgataaaaga tattccgtca tcattaattg agcggcggaa agcagataag
     1501 aaagaagaaa gaaaatgatc tttttcctct tcgaatcggt acggattgcc atgatcgtta
     1561 atagaaaaga taatattaga agggcatatt gaacccaatt ttggttataa gcgatgctga
     1621 acgcctgatg tttaaagaaa ggaaaaatca taaatagaat tgccgaataa gcggcaatat
     1681 cttttctgga aggccataac tttcgaatca ataaccaatt aaagacgccg cctaaatatc
     1741 gccaaaataa tgagtaaatc tgccaaccta atcggttatt cccgacaatc ttaaatccca
     1801 atccgataac ccatggaagc aatccgggac ggctgtcaat cagcgaataa ttaatcagtc
     1861 cgtgaattcc gccttgatca tatgctgtca aataaatcca gtcatccaga taaaatccat
     1921 atcgcggaac ttgaataaac caagtaattc ccattattaa cagcaaagtc aaagggaaat
     1981 atttaccgat tatgaatttt tctgcttttc gcattattgc tccgaattcg atatctctct
     2041 cacttattta aatgagattc tcaaattata tctaaggatc accgtattga tcgtttgtta
     2101 agggaagaat aagcgaaaga ttaagaaatt cgaaaaaatt ctcattattt tgggaaaatt
     2161 ccatttcctt cttaaagaaa aaacaatcga tctttctata tattttataa gaaattctct
     2221 tgtttctt
//
LOCUS       DAOMIV010000355         2219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_55114_length_2219_cov_4.987061, whole genome shotgun sequence.
ACCESSION   DAOMIV010000355 DAOMIV010000000
VERSION     DAOMIV010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55114_length_2219_cov_4.987061"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PKV59_07885"
     CDS             <1..453
                     /locus_tag="PKV59_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46707.1"
                     /translation="SIFCAWRSCSSNLIASVTPESYQGLHAKFVNSYAYTEGGDNGNQ
                     KTFETEYTPNTLFTFDVVFENDGTVNWPPQVEMRNTGSVSTYTGHRPNVIVDTSNNPV
                     KPGDRLGFSISAHGSEELGWHTFYFQLYDAVSGVPIPGGYGYFSYLAK"
     gene            532..897
                     /locus_tag="PKV59_07890"
     CDS             532..897
                     /locus_tag="PKV59_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46708.1"
                     /translation="MAALILLLIILFMLSRIYKHEDRFIFWLTLLFSVGAIFSLSGLL
                     FIQLGPEFTGLPDHSPDAQKTLEYFRMLLFFSWILIGILFSKAFNTPMKQHRRILLGM
                     ILSFPVTTIAGNFIYQLIL"
     gene            943..1512
                     /locus_tag="PKV59_07895"
     CDS             943..1512
                     /locus_tag="PKV59_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46709.1"
                     /translation="MAQDPWLTVDFIWKISGFTVILLIISWIFDHFKTDSRLTRRTVL
                     STGVVVLGYLFFRFLTVPNIEFNETAISILFLTSWILISFLIYKAVKPDMQKLLKGIF
                     SALISFGISISFFNLLIPFKNLSADRQIVNSEYALWVPVIIWIILSGLFYAAFNSGMN
                     RFLRLILGMVCGLAFTAILQPPLLQFLNG"
     gene            1519..1911
                     /locus_tag="PKV59_07900"
     CDS             1519..1911
                     /locus_tag="PKV59_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46710.1"
                     /translation="MPIENLILLEMIAGWSIVTCLVYELSLSISKHQIIRVLFSLIIS
                     LGITFLVWAVFFSGKVNWNVEEGFIGKSDENIFRFVFILLWICVGFLFSKAFNPSMHP
                     TLRLLLGLICSFLVTNYFMETLYLVYFS"
     gene            1945..>2219
                     /locus_tag="PKV59_07905"
     CDS             1945..>2219
                     /locus_tag="PKV59_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46711.1"
                     /translation="MDENILAIIILAVLIFAVFHLLAKSVYAILRIALAVLLTIEILY
                     YLRLLINIPFLNQINWSFIDQLNLRIIQFFKTIFTFGSDAVFRINTM"
BASE COUNT          582 a          442 c          424 g          771 t
ORIGIN      
        1 tccatcttct gtgcctggcg gagctgttcc tccaatctga tcgcctcggt gacaccggag
       61 tcctatcagg gtctgcatgc caaatttgtc aattcctatg cttataccga aggcggtgat
      121 aacgggaacc aaaaaacttt tgagacagaa tatactccca atacactttt tacttttgat
      181 gttgtctttg aaaatgacgg aaccgtgaat tggcctcctc aggttgaaat gcgaaatacc
      241 ggttcggtca gcacttatac cggtcatcgc ccaaacgtaa ttgtggacac ttccaataat
      301 ccggtcaaac cgggagaccg gttgggattc tccatttcag cccatggcag cgaagagctc
      361 ggatggcata cattttattt ccaactttac gatgccgtat ccggtgtacc gattccggga
      421 ggatatggct atttcagtta tttggcaaaa taaaaagcaa tcctgtttaa acgatacctt
      481 ttggagaaat cagacagaaa aatgataacg ggcattgagg aaaatatttt tttggcggca
      541 cttatcctct tattgatcat tctttttatg ctcagccgga tttataaaca cgaagaccga
      601 ttcatttttt ggttgacact tcttttttct gtcggtgcaa ttttctctct ctcgggactt
      661 ctgttcattc agcttggtcc ggaattcacc ggactacctg atcattctcc cgatgcgcaa
      721 aaaacgctgg aatacttccg aatgctttta ttcttcagtt ggattttgat aggtattctg
      781 ttttcgaaag cgtttaatac accgatgaaa caacaccgac gaattttgct cggaatgatc
      841 ctttcctttc cggttacgac tattgccgga aattttatct atcaacttat tttatgaaaa
      901 ggagcgggat gatttttatc agcctgtaaa aaagataacg aaatggctca agatccttgg
      961 ctgaccgttg attttatttg gaagatttca ggatttaccg ttatattatt gatcatcagt
     1021 tggattttcg atcattttaa aaccgattcc cgtctcactc gaagaaccgt tctgagtaca
     1081 ggagttgtgg ttttaggtta tttattcttc cgttttctca ccgttccgaa tatcgagttt
     1141 aatgagaccg cgatttccat attattctta accagttgga tactgatttc atttttaatt
     1201 tataaagcgg taaagccgga tatgcaaaaa ttattgaaag gaatcttctc cgcattgatt
     1261 tccttcggaa taagtatttc tttctttaac ctgttgatcc cctttaagaa cctgtctgcg
     1321 gatcggcaga ttgttaactc ggaatatgca ttatgggttc cggtcataat ttggatcatt
     1381 ctcagcggac ttttttatgc cgcgtttaat tccggcatga atcgttttct ccgactcatt
     1441 ttggggatgg tatgtggatt ggcatttacc gccattttgc aacctccgct tttacaattc
     1501 ctaaacggtt aagtgatcat gcccatcgaa aatctgatct tattggaaat gatagccggc
     1561 tggtcaattg tgacttgcct agtatatgaa ctttctcttt ccatcagtaa acatcaaata
     1621 attcgagtcc tgttttccct gattatcagt ctgggaatta cttttttggt atgggcagta
     1681 tttttctccg gtaaagtaaa ttggaatgtc gaagaaggat tcatcggaaa atccgatgaa
     1741 aatattttcc gcttcgtatt tatcctttta tggatatgcg tcggattctt attttcaaaa
     1801 gctttcaatc cttccatgca tccgactctg agactcctgc tcggcctcat ttgttctttc
     1861 cttgtgacca actattttat ggaaacattg tatttggtat acttttctta gatgcatgta
     1921 gaaaattccc taaaggagtg agaaatggat gaaaatatac tggcaattat catattggca
     1981 gtgttaattt ttgccgtttt tcatctcttg gcaaaatcgg tctatgcaat cttgaggatt
     2041 gctttagcag ttcttctaac aatagaaatc ctttattatt taaggttgtt gatcaacatc
     2101 ccttttctaa atcaaatcaa ttggagtttt atcgatcaat taaatttaag aattatccaa
     2161 ttctttaaaa cgatctttac tttcggatcc gatgcggttt ttcgaataaa tacgatgaa
//
LOCUS       DAOMIV010000356         2219 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_55118_length_2219_cov_4.130314, whole genome shotgun sequence.
ACCESSION   DAOMIV010000356 DAOMIV010000000
VERSION     DAOMIV010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55118_length_2219_cov_4.130314"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..>2219)
                     /gene="mfd"
                     /locus_tag="PKV59_07910"
     CDS             complement(98..>2219)
                     /gene="mfd"
                     /locus_tag="PKV59_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HQP46712.1"
                     /translation="DLKPGDYVVHIDYGIGRYSGLVSRSIDGVAKEFLCIEYAQGDQL
                     FVPVHQADRLTNYIGPDGRAPELTRLGTQEWLSVKQHVSEKVVEVAEDLLDLYAKRQV
                     TPGYAFSPDTTWQMDMEGSFPYIETEDQRRAIRQVKEDMERPRPMDRLICGDVGFGKT
                     EVALRAAFKAACDGKQVAVMVPTTVLAQQHYDTFKQRFASYPVNVEMLSRFRTQKEQD
                     QILKKLAEGEIDVVIGTHRLVSADVKFKDLGLIIIDEEQRFGVAQKEYLKKMRTEVDV
                     LTMTATPIPRTLYMALTGARDISSINTPPDERVPIVTHVGPYSDKLVRQAIMREMDRD
                     GQVFYVHNRVQSIYAIAKHLQELVPEARIGIGHGQLPEKELSEVMESFYNREIDVLLS
                     TSIIESGLDVPNANTLIVDRADTLGLAQLYQIRGRVGRSSQRAYAYFFQQRGSHPTEE
                     GLERLEVIAENTQLGAGFSIAMRDLEMRGAGEILGNKQHGSIAAVGFNLYTRLLAQAV
                     KKVKEDKGLSISDEEIGITKDMAVLFNPITVELPMDIGIPEEYIPEQKTRIKLYRRIA
                     SIHEEEKLIGLKDEFQERFGPLPEIVQNLFFQILVKIKAEKIGLSAVVKEGNSIVLRF
                     SSFANEMERGPLPEVGGGIRPGKNAYWLTLMNFQSDEWRNDLLVAMDLVEDRLAQQRI
                     RSKEFGEQAEKSGVTSFLGIEKSP"
BASE COUNT          532 a          622 c          445 g          620 t
ORIGIN      
        1 gtttcttcat ttctgctgct tctcaattgt caaggttctt cacgcccctc tgttttccca
       61 ggcgcaacgt cttattatat gtgttttccc ctttatgtca aggacttttt tcaattccca
      121 aaaaactcgt tacgccggat ttttctgcct gttccccgaa ttctttagac cggatcctct
      181 gctgagccaa gcgatcctcg actaaatcca tagcgaccaa caggtcgttt cgccattcgt
      241 ccgattggaa attcatcagc gtcagccaat atgcattttt tccgggtcgg atccctcctc
      301 cgacttccgg taacggacct ctttccatct cgttggcgaa ggatgaaaag cgcaacacga
      361 tgctgttccc ctcttttacc actgcggata aaccgatttt ttcggctttt atttttacga
      421 gaatctgaaa aaatagattt tgcacaatct cgggcagcgg tccgaatcgc tcctgaaatt
      481 catcttttaa tccgatcagc ttttcttcct catgaataga tgcaatccgc cgatacagtt
      541 taatccgggt cttttgttcg ggaatatatt cttcagggat accgatatcc atcggcagtt
      601 ctaccgtgat cgggttaaac aaaaccgcca tgtcttttgt gataccaatc tcctcatccg
      661 atatcgaaag tcctttatct tctttgactt tcttcacagc ctgtgctaaa aggcgggtgt
      721 acaaattgaa tccgaccgct gcaatcgaac catgctgttt gtttccgaga atttcccccg
      781 caccgcgcat ctccaaatcg cgcatcgcaa tcgaaaaacc cgcgcccaac tgagtatttt
      841 cggcaattac ttccaatcgc tccaatcctt cctcggtcgg atggctgccg cgttgctgga
      901 aaaaatatgc ataggcgcgt tgggagcttc tgcccactcg gcctcggatc tgataaagtt
      961 gagccaatcc caaagtatcc gcccggtcga caatcaaagt gttcgcattg ggaacatcca
     1021 agccggactc aataatcgat gtactcaaca acacgtcaat ttcccggtta tagaaagatt
     1081 ccatcacctc ggacaattct ttctcgggca attgtccatg accgattccg attcgagctt
     1141 ccgggaccaa ttcctgcagg tgttttgcaa tcgcataaat ggattgaaca cggttgtgaa
     1201 cgtaaaatac ctgtccgtcc cgatccattt cccgcataat cgcttgccgg accagtttat
     1261 ccgaataagg accgacatgg gtaacgatgg gcacccgctc atcaggcggt gtattgatac
     1321 tggagatgtc gcgggctccc gtcaacgcca tatagagcgt cctgggaatc ggcgtagcgg
     1381 tcattgttag tacatcgact tccgtccgca tttttttcaa atactctttt tgagccacgc
     1441 cgaaacgctg ctcctcatca ataataatca gacccaaatc tttgaatttt acatccgccg
     1501 aaaccaaacg atgagttccg atcaccacat caatttcccc ttcagccaac ttctttaaaa
     1561 tttgatcttg ttctttctgc gtccgaaagc gggaaagcat ttccacgttg accggataag
     1621 aggcaaaacg ttgcttgaag gtatcatagt gttgttgggc taaaaccgtc gtcggaacca
     1681 ttactgccac ctgttttccg tcacaggcgg ctttgaaagc tgctctcaag gcaacttccg
     1741 tcttcccgaa accgacatcc ccgcaaatca aacgatccat cggccgcggc cgttccatgt
     1801 cttcttttac ctgacggata gcccgccttt gatcttcggt ttcaatatag gggaaagacc
     1861 cttccatatc catctgccag gtggtatccg gcgaaaatgc ataacccggt gtgacctgtc
     1921 gtttggcata caagtccaac aaatcttcag ccacttccac tactttttca ctgacatgct
     1981 gtttaacgct cagccattcc tgcgttccca atcgggtcaa ttcaggcgcc cgcccatcgg
     2041 gaccgatata gtttgtcaac cgatcggctt gatgaaccgg cacaaacagt tgatctccct
     2101 gagcatattc aatacataaa aactctttgg cgacaccgtc aatggatcgg ctgaccagtc
     2161 cggaataccg cccgattcca tagtcgatat ggacgacata atctcccggc tttaaatcc
//
LOCUS       DAOMIV010000357         2218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_55160_length_2218_cov_4.246417, whole genome shotgun sequence.
ACCESSION   DAOMIV010000357 DAOMIV010000000
VERSION     DAOMIV010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55160_length_2218_cov_4.246417"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKV59_07915"
     CDS             complement(<1..418)
                     /locus_tag="PKV59_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP46713.1"
                     /translation="MESTNTVSIKHLDKIYSNLLKEDTVALKDINLEIKNGEFLSIIG
                     PSGCGKSTLLKVIGGLEKPTRGEIEYLDKENRLGFVFQDSVLLPWKNVRENAEFPLVI
                     QKKQTKENEERLDALLELAGLTEFKTALPRELSGGMK"
     gene            complement(425..1159)
                     /locus_tag="PKV59_07920"
     CDS             complement(425..1159)
                     /locus_tag="PKV59_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HQP46714.1"
                     /translation="MQLIDRACQILTILSYQPEGMSVLELSEKINLAPSTTHRILTSL
                     KENKMVVQDQDSKKYRIGYKILSLCANLNERNTLITAAKPVMKQLSKLIDKTIVLCVM
                     ENTYIMNIACIEKADSNLFLVKIGRELPLFSTSAGRVFCAFMDREKVHSVYESVHEQA
                     LTPYTKTSWDALCSEMDRVKENGYACIDEELQLGIKGYACPIFDINGKVIAALAFLST
                     KASLQDAEPMIRSLKESAKQISTEIG"
     gene            1342..1416
                     /locus_tag="PKV59_07925"
     tRNA            1342..1416
                     /locus_tag="PKV59_07925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1377..1379,aa:Pro,seq:ggg)
     gene            1553..1972
                     /locus_tag="PKV59_07930"
     CDS             1553..1972
                     /locus_tag="PKV59_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQP46715.1"
                     /translation="MTRILFILDGSNFLFGENDADPVQILSDIRNGVFALPFRSDPVK
                     CVEIEGWVVVGLRSRQSMIGYLTQQEINVLELLIQGKTVNQIAVALTLSPETIKYHIE
                     GAKIRMQVETRNELIALYCREKENIQNTQFPLELRKT"
BASE COUNT          606 a          474 c          460 g          678 t
ORIGIN      
        1 gtttcatgcc gccggataat tctcgcggca aagctgtttt aaattccgtc agacctgcca
       61 attccaataa cgcatccagc cgttcttcat tttctttcgt ctgtttcttt tgaatcacca
      121 gcggaaattc ggcgttttcg cgaacattct tccaaggcag cagaaccgaa tcctgaaata
      181 caaaacccaa acggttttcc ttatcaaggt attcgatttc gccgcgggtc ggcttttcca
      241 atccgccgat gacttttaac agagtggatt tgccgcagcc ggaaggaccg ataatcgaga
      301 gaaattcacc atttttgatt tcaagattaa tatccttcag cgcgaccgta tcctctttca
      361 aaaggttgct gtatattttg tccaaatgtt tgatacttac cgtatttgtg gattccatcg
      421 attcctaccc gatttccgtt gatatttgtt tcgcactttc tttcagactc cgaatcatcg
      481 gctctgcatc ttgcagactt gctttggtac tcaaaaacgc cagcgcggca atgacttttc
      541 cgttgatatc aaagattggg catgcgtacc ctttgattcc caattgcaat tcttcgtcga
      601 tacaggcata cccgttttcc tttacccgat ccatctccga acataaggca tcccaagatg
      661 tttttgtata aggagtcaaa gcttgttcgt gaacggattc ataaacggaa tggacctttt
      721 cccgatccat gaatgcgcaa aaaactctgc cggctgaagt ggaaaataaa ggaagttctc
      781 tgccgatttt taccagaaac agattcgaat ccgctttttc gatgcaggcg atattcataa
      841 tataggtatt ttccatcaca cacagaacga tcgtcttgtc gatcaatttg gaaagttgtt
      901 tcatgacagg tttcgcggcc gtaatcaatg tgtttctttc gttcagattc gcacataaag
      961 acaagatttt ataaccgatc cgatatttct tactgtcctg atcctgaaca accattttgt
     1021 tctctttcag gctggtcagg atgcgatggg ttgtgctggg cgccagattg attttctcgg
     1081 ataattcaag aacggacatg ccttccggtt gataactcaa aatagttagg atttgacaag
     1141 cgcggtcgat taattgcatc tttgattcct gccattccgt ataacggaat aagatttctt
     1201 tttatggaat gttaaacgtg attgtactta ttcattgacg atttgtcaat ctgtttaagc
     1261 tctgaatttt ttatttttat cagacctttc aaaatcattt cttgtcgcac aaatgtttca
     1321 gggtataata ttctcgttgt tcggggcata gcgcagcccg gctagcgcgc ttgcatgggg
     1381 tgcaagaggc cctgggttca aatcccagtg ccccgacaga tcaaaaagac cgcagaattt
     1441 ttttctgcgg tctttttgat attgacaatc ccgctgaaat cctctatatt tgtagaacat
     1501 caaaatagaa aataatttct attatttaac ctgaagccaa aggaactgaa ccatgacgcg
     1561 gattttgttt attcttgatg ggagcaactt tctgtttggt gagaatgatg cagacccggt
     1621 acaaatcctt tccgatattc ggaacggcgt gtttgccctg ccttttcgaa gcgatccggt
     1681 gaagtgtgtg gaaattgaag gctgggtggt ggtcgggttg cgcagccgcc aatccatgat
     1741 cggatatctg acgcaacaag aaatcaacgt gctggaactt ctgattcaag gaaaaacggt
     1801 aaaccagatc gcagtcgctt tgacgctgag tcccgagacg attaaatatc acattgaagg
     1861 tgcaaaaatt cgaatgcaag ttgaaacaag aaacgaattg attgcccttt attgtcgtga
     1921 aaaggagaac attcaaaata ctcaattccc cctcgagtta agaaaaactt aagtttcaaa
     1981 aagaaacgaa tcacagcgcg ttggatataa tgaagtcaaa ggagagacgg attttggacc
     2041 ttttttccac tgaacaaagc aataaaacca atctttttaa gggaacgaaa acccctcaat
     2101 attcgtatag aaattggagg gaattatgag aagaaagaat tggatggggg tttactccct
     2161 tttgttgatc gttattttat cgattttttc aggcgctgcc aatccaattt tgcaaccg
//
LOCUS       DAOMIV010000358         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_55250_length_2216_cov_2.415086, whole genome shotgun sequence.
ACCESSION   DAOMIV010000358 DAOMIV010000000
VERSION     DAOMIV010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55250_length_2216_cov_2.415086"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKV59_07935"
     CDS             complement(<1..257)
                     /locus_tag="PKV59_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQP46716.1"
                     /translation="MKDFDFSENEIFAKGNPLPEPLRKNFSGTCYLNPIIPADSPLGC
                     SVSNVTFAPGCRNNWHKHPGGQILLVTGGRGWYQEEGKEVR"
     gene            complement(278..1336)
                     /locus_tag="PKV59_07940"
     CDS             complement(278..1336)
                     /locus_tag="PKV59_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQP46717.1"
                     /translation="MKKTISRREALKRMTWSTAAILIASENIIHPKNTGGQFFAPDFN
                     KKPAKIKTSTNNGTESERGAKMAEKVYMTTEISPIGLMNIYKALGRKADGHVAVKISS
                     GEPGGHYFLSPSLIAHLVSEVKGTIVECNTAYGGERSNTVSHLKVMKEHGFTDIAPVD
                     IMDAEGTMNLPISGGSHLNEDVVGSHFKLYDFIINLAHFKGHAMGGFVGVIKNMSIGI
                     ASSKGKSLIHSAGKETTGFGINTPQDDFLESMAEAAKGVADYLADRILYINVMNNLSI
                     DCDCNSHPSKPTMKDIGILASLNPVALDQACVDLVYAAPDGKDLIERIESRHGIHTLE
                     HAEKIGLGSCKYQLVQID"
     gene            complement(1514..2053)
                     /locus_tag="PKV59_07945"
     CDS             complement(1514..2053)
                     /locus_tag="PKV59_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HQP46718.1"
                     /translation="MNNNILIAYFSRADQNYFGGKIVALPVGNTEVIMKKISEQIGGT
                     LFKINPLKKYSVDYQICTEEAKKELNENARPPLSDYLDKVEIYNPIILGYPIYWGTMP
                     MPVWTFLEHYDFSGKTIFPVCTHEGSGLGRSEEDIRKICPAANVRKGFAIRGSDVNRA
                     QNEIDRWLKEQGLFHSQTD"
BASE COUNT          579 a          486 c          439 g          712 t
ORIGIN      
        1 tcacgaacct cttttccttc ctcttgatac caacctcttc caccggtaac caaaaggatt
       61 tgtccacccg ggtgtttatg ccaattattc cgacacccag gcgcaaaagt gacattactg
      121 acagaacaac ccaatggtga atccgcggga ataatcggat tgagatagca tgtcccggaa
      181 aagttctttc gcaaaggttc cggaagtgga ttcccttttg caaatatttc gttttctgaa
      241 aaatcaaagt ctttcatatg aatgctcctt ttataaatca atcgatttgt accagttgat
      301 atttacagct gcctaagccg atcttttcgg catgttccaa agtatgaatg ccgtgacgcg
      361 attcgatgcg ttcaatcaaa tctttcccgt ccggtgctgc atatacgaga tctacacaag
      421 cttgatcgag tgcaacggga ttaagcgaag cgagaatgcc gatatctttc atagtcggct
      481 ttgatggatg gctgttgcaa tcgcaatcta tggataaatt attcattacg ttgatgtaaa
      541 gaatacggtc agctaaataa tcggccactc ctttagcggc ttccgccatc gattccaaaa
      601 agtcatcctg aggtgtgttg atcccgaaac cggttgtttc ttttccggcc gaatgaatta
      661 agcttttccc ttttgacgaa gcgattccga tggacatatt cttgattaca ccaacgaatc
      721 cacccatggc atgaccttta aaatgggcta aattgattat aaaatcgtaa agtttaaaat
      781 gtgatccaac gacgtcttcg ttcaagtgac ttcctcccga aattggcagg ttcatagttc
      841 cctcggcatc catgatatcg acaggggcaa tatctgtaaa gccatgttct ttcataactt
      901 ttaagtgaga aacggtattt gaacgctctc ctccgtaggc ggtattgcat tcgacgattg
      961 ttcccttgac ttcggaaacg agatgggcga ttaatgacgg agaaaggaag taatgcccgc
     1021 ccggttctcc actggagatt ttcaccgcaa cgtgaccatc ggcttttcga ccgagtgctt
     1081 tataaatatt catcagacca ataggactga tctctgtcgt catatatact ttttcagcca
     1141 tttttgctcc tctttcggat tctgtaccgt tatttgttga tgtctttatt tttgcaggct
     1201 ttttattgaa gtccggcgca aagaactgcc cgccggtgtt tttcggatga atgatatttt
     1261 cggatgcgat aagaattgca gccgttgacc aagtcatccg tttcagcgct tccctgcgag
     1321 aaattgtttt tttcatccga gtttctatcc tttccgggtc gacatcattg aatcgcttta
     1381 tttgctcttg atcggctctt gatctgtttt tcagtgtaat acttggagct tactctaagt
     1441 caagggtatt tttaaagaat ttttggatgt aaaggaaaat ccaaaaaaga tctttttctg
     1501 ttcactttta tggtcagtct gtttggctgt gaaataaacc ttgttctttc agccatcgat
     1561 caatctcatt ttgggcacga tttacatcac tgccgcgaat ggcgaatcct ttacgaacat
     1621 ttgccgccgg acaaattttt cgaatatcct cttcgctgcg tcccaaaccg ctaccttcat
     1681 gtgtacaaac cgggaaaatt gtttttcccg agaagtcata atgctcgagg aaagtccaaa
     1741 ccggcatcgg cattgttccc caataaatgg gataaccgag aataatcggg ttgtaaatct
     1801 ctaccttatc taaataatcg cttaatggtg gtctggcatt ttcattcagt tctttttttg
     1861 cttcttctgt gcagatctga taatccactg aatatttttt aagcggattg attttaaaca
     1921 gtgtcccccc gatttgctcc gaaatctttt tcataataac ttcggtgttc ccgaccggca
     1981 acgcgacgat ttttccccca aaataattct ggtctgcccg agaaaaatat gcgatcaaaa
     2041 tattgttgtt catatatgct ttcctttagt tgtaaaagtc attgtaaatc ctggagctaa
     2101 ctccaagtca agtggattat tgtgaaaaat agacgaccca aattaatctc aaaaaaatcg
     2161 gctgaatttg agccgatttt tttgagaagc gttgagagtt ttaccgttgc agattt
//
LOCUS       DAOMIV010000359         2205 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_55670_length_2205_cov_2.685116, whole genome shotgun sequence.
ACCESSION   DAOMIV010000359 DAOMIV010000000
VERSION     DAOMIV010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55670_length_2205_cov_2.685116"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PKV59_07950"
     CDS             <1..715
                     /locus_tag="PKV59_07950"
                     /inference="COORDINATES: protein motif:HMM:NF039236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46719.1"
                     /translation="PTYEEHGLIWLWWGDSKPDGIPDFFTDIDDTFYYRSAHDPWDAF
                     YSRVIENQLDVIHLPFVHRHTIGRGNRTLVDGPLVEWKNDRMIYTYVFNRVDDGTLPR
                     KPKELTPKSATSVHLEFIFPNLWQNFINEKVRVLAAFVPVDNDHTILYLRFYQKFLPV
                     PILGKMVANLAMPVNVYIAHEDRRIVITQQPKISGLKIGETLLQGDLPVIEYRRKRAA
                     LQEKNESKTVSEAPKYTDR"
     gene            791..>2205
                     /locus_tag="PKV59_07955"
     CDS             791..>2205
                     /locus_tag="PKV59_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQP46720.1"
                     /translation="MENNRKNYYDAIVIGGGLAGLTAASFLAKEGRKVLVCEQSDQVG
                     GLFNSFWRSGYLFDGGIKAIENSSVMMPMLAKLGLLEQVHFHPSPIALIIGGNIQVIR
                     QFEDVVCYFRMLQEFFPSEKAGLKKILQDTKTVYDLMDGFLSFPIPFFDLPNTGNEAR
                     AEWFRKQGALFTRIPRALSLMRQDFRPYLEKHLNNSNLINILSDLFPDGTSVFFGLGY
                     FRMFTDYYYPEGGIRTIPHLLSESLQNWGGEIRLNTRVENLLFDCGRASGVRLITGEE
                     IRADYMIAAGDLRQALTQLTPETMFPKKFNRKLKDAEVSHSVFNVFLGIDRPVESLGL
                     QGCSHVFYHPDLNGISENDRIHRPDYFRHVPQEISVPCLHQNELAPQGKTGMMISAMT
                     SWDYNGGWEREPAEYENLKEKYTSDLIASLEKFIPQLNDHIEICFSATPLTIARRTSN
                     TKGAIMGWSYHKERTFSRGNF"
BASE COUNT          622 a          469 c          497 g          617 t
ORIGIN      
        1 tcccacctat gaagagcacg gcttgatctg gctctggtgg ggtgattcga aaccggacgg
       61 aatccccgat ttttttaccg atatcgatga taccttctat tatcgttccg ctcatgatcc
      121 ttgggatgct ttttactcaa gggtaatcga aaatcagttg gacgttatcc acttgccatt
      181 tgttcatcgc cacacaatcg gccgcggaaa tcggacttta gtcgacggtc cgctcgttga
      241 atggaaaaat gacaggatga tctataccta tgtgttcaat cgggttgacg acggcaccct
      301 tccgcgaaaa cccaaagagc ttactccaaa atccgcgaca agtgttcatt tggaattcat
      361 ttttcccaat ctgtggcaaa attttatcaa cgagaaagtg cgtgttttag cggcattcgt
      421 cccggtggat aatgatcata caattcttta cctcaggttt tatcagaaat ttttaccggt
      481 tccgatttta ggaaaaatgg tagcaaattt ggcaatgccg gttaatgttt atattgcaca
      541 tgaagaccgc cggatcgtca ttacacaaca gccaaaaata agcggcttga aaatagggga
      601 aaccttgctt caaggagatt tgccggttat cgaatatcga cgaaaaagag cggctttgca
      661 agaaaaaaat gaaagcaaga ccgtttctga agcaccaaaa tacaccgacc gataattttt
      721 tgtccgatga attcagttca tctttttgct gctcaagata atattgtttt ataaaatcgt
      781 tgggatagac atggaaaata atcgaaagaa ttactatgat gcgatcgtta tcggcggagg
      841 acttgcggga ctcacagcgg cttcattctt ggcaaaagag gggagaaaag tattggtttg
      901 tgaacagtcg gatcaagtgg gaggactgtt caattcattt tggcgatcgg ggtatttatt
      961 tgacggagga ataaaagcta tagaaaacag ctcggtcatg atgcccatgc tggcgaaact
     1021 cggattgttg gaacaggttc attttcatcc aagcccgatc gctcttatta tcggcgggaa
     1081 tatccaagtt attcgacaat ttgaagatgt tgtttgctat tttcgcatgt tgcaggaatt
     1141 ctttccctcg gaaaaagccg gcttgaagaa aatccttcaa gatacaaaga cagtctatga
     1201 tttgatggat ggcttcctgt cttttccgat tcctttcttt gatttaccga ataccggcaa
     1261 tgaggcacga gcagaatggt ttaggaaaca aggtgcttta ttcacgcgta tcccccgcgc
     1321 attatcgctg atgcgccagg attttcgacc ttatttagaa aaacatctca acaattcaaa
     1381 ccttatcaac atactcagcg atcttttccc ggacggaacg agtgtcttct ttggattggg
     1441 gtattttcgt atgtttacgg actattatta tccggaagga ggcatccgga ctattcctca
     1501 tctactctca gaatccttac aaaattgggg aggagaaatc aggctgaaca cgcgggtgga
     1561 aaatttgctt ttcgattgcg gtagagcttc cggtgtccgg cttataaccg gagaagaaat
     1621 tcgagcagat tatatgatcg ctgccggaga tctacgccaa gcgctcactc aactgacacc
     1681 ggaaacaatg ttcccgaaga aatttaatcg aaaattaaaa gatgcggagg tctcacactc
     1741 cgtattcaac gttttcttgg gtatcgaccg acccgtcgaa agtttaggcc ttcaaggttg
     1801 ttcgcatgtt ttttatcatc cggatttgaa cggtatctcg gaaaacgacc ggattcatcg
     1861 ccccgattac tttcgtcatg ttcctcagga gatttccgtc ccatgcctcc accaaaatga
     1921 gcttgctcct cagggtaaaa cgggaatgat gatcagcgca atgacatcct gggattataa
     1981 cggcggttgg gaaagagaac ctgcggaata tgagaattta aaagaaaaat atacatccga
     2041 tttaatcgcc agtttggaaa agttcattcc ccagctaaac gatcatatcg aaatatgttt
     2101 ctcggcgaca ccgctcacaa tagcccgacg tacgtccaat acaaaaggag ccattatggg
     2161 ttggtcttat cacaaggaac ggactttctc acgagggaat ttctt
//
LOCUS       DAOMIV010000360         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_56021_length_2196_cov_3.940215, whole genome shotgun sequence.
ACCESSION   DAOMIV010000360 DAOMIV010000000
VERSION     DAOMIV010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56021_length_2196_cov_3.940215"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1173)
                     /locus_tag="PKV59_07960"
     CDS             complement(13..1173)
                     /locus_tag="PKV59_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46721.1"
                     /translation="MMKINKNKKRIGVIGTGFIAKGFVLTLTNHRDFSVSKVLTRRTK
                     RECREYPRKDVLTNSVNELMEDSDLIVECSGDAIHAANVIDQALKMKKPVVTMNTEFH
                     VTAGSYFVGKGLVTEAEGDQPGCLAALHEEVTQMGFEPLVYGNVKGFYNPNPTEEEMK
                     FWAKKQGLSLPMVTASTDGTKVQYEQALVANMFKAAIVKTGLVGPKTEDVKTSAFELV
                     KYARRLKQPISDYVINGRSPVRVFIVAKGDSRQRKALEYLKMGKGPVYYFDKNSILTY
                     MEIIKTVRRVLNGGGILLDNGQVPTVSIAAVAKHGICKGTKIEHPVGNFDMRGVAVEI
                     KKVKDHVPMALLLNAVFKRKVNQGAIVCWDDVEIPESLALKAWRSIAQRVLK"
     gene            complement(1295..1867)
                     /gene="rfbC"
                     /locus_tag="PKV59_07965"
     CDS             complement(1295..1867)
                     /gene="rfbC"
                     /locus_tag="PKV59_07965"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HQP46722.1"
                     /translation="MAFKFEKCEIPGLLIVEADVFNDTRGFFIETYQQSAFINAGIDK
                     PIVQIDHSRSKKNVVRGLHYQLNPMAQGKIVDVIDGEIFDVTVDLRKGSPSFGKWFGI
                     NLGIGQGKLLYIPEGFAHGVCALSETADIIYYCTQVYAPEYQRGILWNDPELDITWPV
                     KKPVLSDKDKQLPLLNQADNNFVYEKIINT"
     gene            complement(1884..>2196)
                     /locus_tag="PKV59_07970"
     CDS             complement(1884..>2196)
                     /locus_tag="PKV59_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46723.1"
                     /translation="QRFNVGSNDQNFKVFDLAKLVAESAGVPFDYEWYGDPDHRSYQV
                     DFSKVKRVVGFEPVFSPRKAVPEIWAALENGSLDPTALTSITLNWYKKLIAEGVKIGM
                     E"
BASE COUNT          590 a          587 c          383 g          636 t
ORIGIN      
        1 cctcccttaa aattacttta aaaccctctg cgcaatggat cgccaggcct ttaacgctaa
       61 actttccgga atttcaacat catcccagca aacaatagcg ccttggttga ctttgcgttt
      121 aaaaacagcg ttcaacaata acgccatggg cacatggtct ttaacctttt tgatctccac
      181 ggccacccca cgcatatcaa aatttccaac gggatgttcg atctttgttc ccttgcaaat
      241 cccgtgtttt gccacggccg cgatactcac ggtcggaacc tggccgttat ccagtaaaat
      301 gccccctccg tttaagacgc gccgcactgt cttgataatt tccatatagg taagaatgct
      361 gtttttatca aaataataaa cagggccctt acccattttt aaatattcaa gggccttacg
      421 ctgcctggaa tcgcctttgg ccacgataaa cacacgtaca ggcgatcgcc cgttaatgac
      481 gtaatcactg atgggctgtt tcaatctccg cgcatattta acaagctcaa aagcggatgt
      541 tttgacatcc tcggtcttgg ggccaaccaa tcctgttttg acaattgccg ccttgaacat
      601 gttggctact aaagcctgct catactgcac ttttgtcccg tccgtcgaag ctgtgaccat
      661 gggcaagctc aatccctgtt tcttagccca gaatttcatt tcctcttctg tcgggttagg
      721 attataaaat cccttcacat tcccgtacac caaaggttcg aaccccattt gcgtcacttc
      781 ttcatgtaac gcggccagac atccaggctg atccccctcc gcttcggtca ccagaccctt
      841 cccgacaaaa tagctgcctg ccgtcacatg aaattcggtg ttcatggtca ccacaggttt
      901 cttcatttta agcgcttgat cgatcacatt ggccgcgtgg atggcatccc cgctgcattc
      961 cacaataaga tccgaatcct ccatgagctc attgacagaa ttcgttaaaa catcctttcg
     1021 gggatactct ctgcattcac gttttgtgcg ccgcgtcaat actttagaga ccgaaaaatc
     1081 tctatgattg gttaatgtca aaacaaaacc cttggcaata aatcccgttc cgatgacccc
     1141 gatgcgtttt ttatttttat taatcttcat cattttatta tttcctataa ctatcccaac
     1201 tatgtcattc tgaggaacaa cttacttata actaccagtg acgaagaatc tcaaaaaaga
     1261 gattcttcgc cctacgggct cagaatgacg aaatttaggt attaataatt ttttcataaa
     1321 caaaattatt gtctgcttgg ttaagcagcg gtaattgctt atccttatcg gacaagaccg
     1381 gttttttgac cggccaggta atatctaatt ccggatcatt ccaaagaatg cctctttgat
     1441 attcgggagc atagacctgc gtgcaataat agataatatc ggccgtttct gacaaagcgc
     1501 aaaccccgtg ggcaaatccc tccggaatat acagcaactt tccctgacca atccctaaat
     1561 taatcccaaa ccattttcca aaagaaggcg aaccttttct taaatcaacc gtcacatcaa
     1621 atatttcgcc atcaatgaca tcaacgatct ttccttgggc catagggttc aactgataat
     1681 gcaatcctct gacaacgttt tttttgctcc tcgagtgatc aatctgcaca atcggcttat
     1741 cgataccggc attgataaat gcgctctgct gatacgtctc aataaaaaaa cccctcgtat
     1801 cattaaaaac atccgcttca acaatgagca gtcctggaat ttcacatttt tcaaatttaa
     1861 atgccatttt attctttccg tccttattcc atcccgattt taacgccttc tgcaattaat
     1921 tttttatacc aattcaaggt aattgaagtt aatgccgttg gatcaagcga accattttcc
     1981 agagccgccc agatctccgg cacggcttta cggggcgaga aaaccggttc aaagccaacg
     2041 acccttttca cttttgagaa atcaacctga tacgaccgat ggtccgggtc cccataccat
     2101 tcataatcga acggaacgcc ggcgctttcg gccaccagtt tcgccagatc aaatacctta
     2161 aaattttggt cattgcttcc cacattgaac cgttgc
//
LOCUS       DAOMIV010000361         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_56422_length_2185_cov_3.325352, whole genome shotgun sequence.
ACCESSION   DAOMIV010000361 DAOMIV010000000
VERSION     DAOMIV010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56422_length_2185_cov_3.325352"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PKV59_07975"
     CDS             <1..771
                     /locus_tag="PKV59_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46724.1"
                     /translation="SGSDPRQFARQMVEHLRMILVIQMGSRKPLEITAEIQSILAKQS
                     AKLTVDQVIEMIRLFDQAVTDSRTGWYPGLSLELATAESCEEKKPVQPVFIQSPVIND
                     QPAKKAASVLPDEEELTEEADDYAGAKVFIRKGQQPDPSVTKEEIEKNWTKVRELIKK
                     HDAILDATLNHAQLLEVRDGVLYLGFPRETIKNQMENSRKSMLWTSAAISNVLGKPVG
                     VALTILGKKNRQPESQGPLVNAALQLGGKLVINKEKTK"
     gene            1012..1254
                     /locus_tag="PKV59_07980"
     CDS             1012..1254
                     /locus_tag="PKV59_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQP46725.1"
                     /translation="MAEATVTATVGGGAVKITMTGDQNCKSVEIDPDFLKDADAEMLQ
                     DMILSGINLALEQSRKLSEEKMSSISNTLSSMGLGF"
     gene            1259..1864
                     /gene="recR"
                     /locus_tag="PKV59_07985"
     CDS             1259..1864
                     /gene="recR"
                     /locus_tag="PKV59_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQP46726.1"
                     /translation="MANIPAPVQNLIDAFSMLPGIGPRTASRLTFFLLKNNTDIAQKL
                     STALMNLKTETALCKECFNITLAGHDLCAICENDQRDQHELCVVETPMDVIVLEQSGG
                     YFGKYHVLHGLLSPIEGINPEDIRLAPLFERVATGDYKEVIIATNPSMEGDATALYIR
                     QRLEPYHVRITRLARGLPSGGELEYADQNTLLRALSGRQEM"
     gene            complement(1955..>2185)
                     /locus_tag="PKV59_07990"
     CDS             complement(1955..>2185)
                     /locus_tag="PKV59_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46727.1"
                     /translation="RMEIEGKYLYDLDKDYDSAQIVLPGQSIRWKIRMEAPKEKTTED
                     DKYYTTYSLVKGTDYDNSRFCPFSLYIYVPHK"
BASE COUNT          682 a          451 c          519 g          533 t
ORIGIN      
        1 tccggctcgg atcccagaca gtttgctcgc caaatggtgg agcatttgcg gatgatattg
       61 gttatccaaa tgggttccag aaagcctttg gaaataacgg ctgaaattca aagtattttg
      121 gcaaaacaat ccgctaaact gacggtggat caggtgatcg aaatgatccg gctgtttgat
      181 caagcggtaa cagacagtcg caccggttgg taccccgggc tgagtttgga actggcgaca
      241 gccgaaagtt gtgaagaaaa gaaaccggtc caacccgttt ttatccaatc gccggttata
      301 aacgaccagc cggctaaaaa agcagcgtcc gttctgccgg atgaagagga acttacggaa
      361 gaggcggatg attatgccgg tgccaaagtt tttatccgga aaggacaaca accggatcct
      421 tcggttacca aagaagaaat cgaaaaaaat tggacaaaag tccgagaact gataaaaaaa
      481 catgatgcga ttctggatgc gactttaaat catgcgcagc tgctcgaagt tcgagacggc
      541 gttctctatc tcggattccc gcgagagaca attaagaatc aaatggagaa cagccggaaa
      601 agcatgctgt ggaccagcgc tgccatctca aatgtgttgg gaaaaccggt cggtgtggcg
      661 ttgacaattc tgggcaaaaa aaaccgccaa cctgaatctc agggaccgct ggttaatgcc
      721 gcactgcaat taggcgggaa actggtcatc aacaaagaaa aaacaaaata gacagaaaca
      781 attcatttta gaatacagat cggtaagtac caaagaattt aattcggaaa tctgggtaaa
      841 atacaacaga taattgaaaa gttgaataaa agaaaataac gaaaaatcag aggagagaag
      901 caaatggcta aaggatacgg aagaccgggc ggcagacctg cggggaatca aaacgcgatg
      961 aggcagcagc tgattgagct gcaaaagcaa atgtaagaag aacaggcaaa aatcgccgaa
     1021 gccactgtta ccgcgacagt gggaggtggg gcggttaaaa tcaccatgac aggcgaccag
     1081 aactgcaaat ccgttgagat agatccggat tttctcaaag atgccgatgc cgaaatgctt
     1141 caggatatga ttttaagcgg cattaatttg gctttggaac agagccgtaa actttccgaa
     1201 gagaaaatga gctcgatcag taacacatta tcttccatgg ggttgggttt ttaatcaaat
     1261 ggcaaatatt cccgctccgg ttcaaaactt gatcgatgct ttttcaatgc ttcccggaat
     1321 cggccctcgt accgcttcac gattgacttt ttttctgctg aagaacaaca cggatatcgc
     1381 gcaaaaatta tccaccgcat taatgaattt gaaaaccgag acagcattat gcaaggaatg
     1441 ttttaatatc accttggcag gccacgattt atgcgcaata tgtgaaaatg accaacggga
     1501 tcaacatgaa ttgtgtgtcg tggaaacccc tatggatgtt attgtgctgg aacaatccgg
     1561 cggctacttt ggaaaatatc atgtccttca tggacttctg tcaccgatcg aagggatcaa
     1621 tcccgaagat attcgtttag ctcctctttt cgaacgagtt gcgacaggtg attataaaga
     1681 agttattatc gcgacaaatc cttcgatgga aggagatgcc accgctttgt atatccgcca
     1741 acgtttggaa ccttaccatg tccgtatcac ccgtctggca agagggctgc catcgggagg
     1801 cgagttggaa tatgcagacc aaaatacctt gttacgcgcc ttatccggta gacaagaaat
     1861 gtgattcgat ttcttattta taaagcgaga taagaaatgc ggctctcagg gagccgcatt
     1921 tctctttcta ttaatcattc agatagacaa aaaatcattt atgcgggaca taaatataaa
     1981 gggaaaacgg acagaatctg ctgttatcat aatccgttcc tttaacaagg ctgtaggtgg
     2041 tgtaatactt atcgtcctct gtagtctttt ccttgggagc ttccattcgg attttccatc
     2101 gaatcgattg tccgggtaaa acaatttggg cggaatcgta atctttatca agatcataaa
     2161 ggtactttcc ttcaatttcc attcg
//
LOCUS       DAOMIV010000362         2184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_56463_length_2184_cov_3.422264, whole genome shotgun sequence.
ACCESSION   DAOMIV010000362 DAOMIV010000000
VERSION     DAOMIV010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56463_length_2184_cov_3.422264"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1419
                     /locus_tag="PKV59_07995"
     CDS             310..1419
                     /locus_tag="PKV59_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HQP46728.1"
                     /translation="MDQSDKKNRTIGAVSFGLLGGWLGYYFSRSVTTSNLYPIRVLIV
                     SAIIGAVLGFFLVVPLIVHPVRRLRVKLENESSEKVAAATIGLIFGLVAAALFSIPAS
                     FLPDPLGEVLPFLIALLLGYLGVYAGISKINWFRKFMPPRFFEPEESDEKITLVDSSA
                     IIDGRIAEIVRTGFLQGKLVVPNFVLQEIQFIADSEDILKRQRGRRGLEVLRQLQKEF
                     PAQVEIRDIAVENIRDVDGKLIALAKKINCPILTDDYNLNQVANLQGISILNINDLSN
                     AVKSVILPGETLMIHVLQEGKEYNQGVGYIEDGTMVVVENGRMYIGQEISVMVTKIHQ
                     TAIGRMIFAKPETEMEQQPYEYTNIQYADEKKRTV"
     gene            1370..>2184
                     /gene="cysS"
                     /locus_tag="PKV59_08000"
     CDS             1370..>2184
                     /gene="cysS"
                     /locus_tag="PKV59_08000"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQP46729.1"
                     /translation="MSIQIYNTLTRKKEPFETLEPHKVKMYVCGPTVYNKAHIGHAMS
                     ALVFDIIRRYLEYRGYEVQFAMNFTDVDDKIINRANEMHVDPFRLAEGYIEDFKRNLA
                     DLNIKPATYNPRASQEIDQIIKMVQGLIDKGMAYPLDGDVYFRVRKDSQYGKLSGRQL
                     DDMLVGVRKEADDRKEDPLDFALWKSAKPGEPSWPSPWGPGRPGWHIECSAMNYHYFG
                     ESIDIHGGGNDLIFPHHENEIAQSESFTGKPFARYWIHNGMLQLRGEKMSKSIG"
BASE COUNT          635 a          404 c          537 g          608 t
ORIGIN      
        1 agacgggttt cgagaaaatt cacaggtatt atggttcccg agaaataacc ccaagatttt
       61 ccgattaaac ggcagctttc agagcgtttt tggttttgaa tttacagata ttccgtccgg
      121 cggaataagg atatgttata ataaaaatat tattataaat tgttattata tttgtctgac
      181 aagagatcga aatttgtatt actttttaat tggaatcatt aaaccaaata ataagtcgaa
      241 aaagatttag gcccgtcaga gttctatatt ctttttttaa attaaggaat atattcaaag
      301 ggaaaactta tggaccagag tgacaaaaaa aatcggacaa tcggagcggt ttcatttggt
      361 ttattaggcg ggtggttggg ttattatttt tcccgttccg taacaacttc aaatttatat
      421 cctatccggg tgctgattgt ttcggcaatc atcggagccg ttttgggctt tttccttgtt
      481 gtgcctctta ttgttcatcc ggttcggcga ttgcgagtta agcttgaaaa cgaatcttcc
      541 gagaaagttg ctgcggctac catcggttta atcttcggat tggttgccgc ggcgcttttt
      601 tcgattccgg cttcattttt gccggatcct ttgggagaag ttctgccttt cctgatcgct
      661 ttgctgctgg gctatctcgg ggtgtatgcg ggtatatcaa aaataaattg gtttcgaaag
      721 tttatgccgc cccgattttt tgaaccagaa gaatcggatg aaaaaattac actggtcgac
      781 agcagtgcga ttatcgacgg gcgaatcgcg gaaatcgtac gaaccggttt tttacaggga
      841 aaattggtag ttccgaattt tgtgctccag gaaatacaat ttattgccga ttcggaggat
      901 atactaaaac gacagcgggg acgacgcggt ctggaagtat tgcgccaact tcaaaaagaa
      961 tttccggctc aggtcgaaat tcgcgatata gcggttgaaa atattcgtga tgtcgacggg
     1021 aaattgattg cgttggcgaa gaaaatcaat tgtcccattc ttacggatga ctataatcta
     1081 aaccaggttg ccaacctgca ggggataagc attttgaaca taaatgatct ctcaaatgcg
     1141 gtaaaatcag tcatacttcc gggtgaaacg ctgatgattc acgttctgca agaaggaaaa
     1201 gaatataatc agggggtcgg atatatagag gatgggacga tggtcgtcgt ggaaaacggt
     1261 cgcatgtata tcggacaaga aatcagcgtt atggtgacaa agattcatca aacggcaatc
     1321 ggaaggatga tttttgccaa acctgaaaca gaaatggaac agcaacccta tgagtataca
     1381 aatatacaat acgctgacga gaaaaaaaga accgtttgag acactggaac cgcataaagt
     1441 aaagatgtat gtatgcggac ccaccgttta caataaagcg cacatcgggc atgcgatgtc
     1501 tgccttggtt tttgatatca ttcggcgata tctggaatat cgcggatatg aggtccaatt
     1561 tgccatgaat tttacggatg tagatgacaa aatcatcaac cgggcgaatg agatgcatgt
     1621 cgatcctttc cgtttagccg aagggtatat cgaagatttt aagagaaatt tggcggattt
     1681 gaacattaag ccggctacct ataatccgcg ggcttcccag gaaatcgatc agattattaa
     1741 aatggtacaa ggtttaatag acaaaggaat ggcttaccct ttggacggag atgtttattt
     1801 tcgagttcga aaagacagcc aatacggaaa actttcggga agacagcttg atgatatgct
     1861 ggtcggcgtt cggaaggaag cggatgatcg aaaagaagac ccgttggact ttgcgttatg
     1921 gaaatccgcc aagcccggcg agccttcttg gccaagtccc tggggaccgg gcagaccggg
     1981 gtggcatatc gagtgttccg ctatgaatta ccattatttc ggggagtcga tcgatattca
     2041 cggcggcggg aacgacctga ttttcccgca tcacgaaaac gaaattgcgc aaagtgaaag
     2101 ctttacgggg aagccgtttg cccgttattg gattcataac ggaatgttgc aattgcgcgg
     2161 tgaaaaaatg tccaaatcca tcgg
//
LOCUS       DAOMIV010000363         2180 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_56610_length_2180_cov_4.652235, whole genome shotgun sequence.
ACCESSION   DAOMIV010000363 DAOMIV010000000
VERSION     DAOMIV010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56610_length_2180_cov_4.652235"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PKV59_08005"
     CDS             complement(<1..276)
                     /locus_tag="PKV59_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HQP46730.1"
                     /translation="MSIYLLDGHALAYRAYFALTAGSGPDRWRTSKGEPVAGIYGFAS
                     ILMRLLDKEKPAHIAIAFDTGKTFRNDLYAEYKATRAKMPDDLRPQID"
     gene            complement(280..1350)
                     /gene="gmd"
                     /locus_tag="PKV59_08010"
     CDS             complement(280..1350)
                     /gene="gmd"
                     /locus_tag="PKV59_08010"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQP46731.1"
                     /translation="MKKALITGITGQDGSYLAEFLLEKGYEVHGIVRRASTFNTRRID
                     HLYNLSQAAEDPIPLFLHYGDMSASESLLDIIYNVSPDEIYNLAAQSQVRVSFDMPEY
                     TGDTTGLGTIRILEAMRKTNSKARFYQASTSELYGKAAPPQNEETPFEPQSPYAAAKL
                     YAYWITRNYRDGYGMFACNGILFNHESPRRGETFVTRKITRAVAMIMAGLQRSLYLGN
                     LDSKRDWGYAPEYVEAMWKILQQDKADDFVLGTGEAHTVREFLDEAFGYVGLDWNQYV
                     KIDSRYFRPTEVDYLLADASKAKRVLNWEPNIKFHELVRIMVDADLEALGLESPGEGR
                     KILNKKFGNWHRWEDQVISMER"
     gene            1611..>2180
                     /locus_tag="PKV59_08015"
     CDS             1611..>2180
                     /locus_tag="PKV59_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46732.1"
                     /translation="MLNGNPLLKNWNGGFFTALTGDILWAAFFRLFLSQKAVLYLIGP
                     LSYALIVLFCFLILSRNQPHHRQKYIGLFLIPILFVPKALRHPLMTLGMHGIAILYIL
                     ILTWLFGILAAENKSSLLMRIVYFFLLWMASIADGYVIYYFAIPALSVLILDAIQSRS
                     KKNRILFFLTLLGTVAGVATIQLINRMGFL"
BASE COUNT          536 a          539 c          444 g          661 t
ORIGIN      
        1 gtcaatttgt gggcgcaaat catccggcat tttcgcccgt gtcgctttat attctgcata
       61 taaatcgttt cggaaagttt ttcccgtatc aaaggcaatg gcaatatgag ccggtttttc
      121 cttatccagc aaccgcatca ggatactggc gaatccgtag attcccgcca ccggttcacc
      181 cttactggtt ctccagcggt ccggccccga cccggctgtt aaggcaaagt aagcccggta
      241 agcaagcgca tgaccatcca aaagatatat gctcatggct cagcgttcca tactgatcac
      301 ctggtcctcc caacggtgcc agtttccgaa tttcttattg agaatttttc ttccttcgcc
      361 gggagattcc aatcccaatg cttccaaatc cgcatccacc ataattctga ccaattcgtg
      421 aaatttaata ttcggttccc agttcagaac tcgtttcgct ttggatgcat ccgccaacaa
      481 atagtccact tcagtcgggc ggaaatagcg ggaatcgatt ttcacatatt gattccaatc
      541 caacccgaca taaccgaaag cttcatccag aaactcacgt actgtgtgtg cctcgccggt
      601 ccctaaaaca aagtcatcgg ccttatcctg ctgcaaaatc ttccacattg cttcgacata
      661 ctccggagca tatccccaat cacgttttga atccaaattt ccgagataaa gtgatcgctg
      721 caaaccggcc ataatcatgg caactgcccg ggttatttta cgagtcacaa aggtttctcc
      781 tctgcgcgga gattcatgat tgaagagaat accgttacag gcaaacatcc cgtaaccgtc
      841 ccgataattg cgagtgatcc aataagcata aagtttggct gccgcataag gactttgcgg
      901 ctcaaacggc gtttcttcgt tttgcggagg agccgcttta ccgtaaagtt cactggtgga
      961 tgcttggtaa aaacgtgctt tggagtttgt tttccgcatg gcttcgagaa tgcgaatcgt
     1021 cccgagaccc gttgtatccc ccgtatattc gggcatatca aaactgacgc gaacttggct
     1081 ttgtgccgcc aaattgtata tttcatccgg tgatacattg taaataatat ctaataaaga
     1141 ttcggaggct gacatatcgc cgtagtgtaa gaaaagcgga atcggatcct cggcagcctg
     1201 cgacaggttg taaagatggt cgatccggcg tgtattaaaa gtacttgctc tgcggacgat
     1261 cccatgtact tcataccctt tttccaacag gaattcggct aaataggaac cgtcctgccc
     1321 tgttattccc gtaataagtg cttttttcat tgattaccct ccctgtcccc tttttgaatt
     1381 atagcggata tttcaatcgg tcagacatag ttgcctgtcg ctgttcgaaa acggaatgaa
     1441 ccggatcgaa tatacttaga tccatgaaag aattttttca aacaaaaaag ctcattttga
     1501 tttccggttt gaccttttta tgtttattaa tcggatatta tatttatttt tatagctttt
     1561 cattcccggt caattcagat aacgtatatt ttcttcatgc agccgaagac atgttaaacg
     1621 gaaatccttt attaaagaac tggaacggcg gcttcttcac cgcattgact ggcgatatct
     1681 tatgggctgc cttcttccga ctttttctga gccaaaaagc ggtgctctat ctgatcgggc
     1741 ctctttctta tgcgctgatc gttttgtttt gcttcttgat cttaagccga aatcagcctc
     1801 accatcggca aaagtacatc ggactttttc tgatacccat tttgtttgtt ccaaaggcac
     1861 tgcgccatcc cttaatgacc ttggggatgc atggtatcgc gattctttat atattgatat
     1921 taacatggct gttcggcatt ctagcagccg aaaacaaatc gtccctcctc atgagaatcg
     1981 tttatttttt cctgctctgg atggcttcga tagcggacgg atatgtgatt tattactttg
     2041 cgattcccgc actttcggtg cttattttgg acgcgattca atcccgttca aaaaagaatc
     2101 ggattttatt cttcctcacc cttttgggaa ccgtcgcggg cgtcgccacc attcaattga
     2161 ttaaccgtat gggctttctc
//
LOCUS       DAOMIV010000364         2172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_56927_length_2172_cov_3.906944, whole genome shotgun sequence.
ACCESSION   DAOMIV010000364 DAOMIV010000000
VERSION     DAOMIV010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56927_length_2172_cov_3.906944"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKV59_08020"
     CDS             <1..350
                     /locus_tag="PKV59_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HQP46733.1"
                     /translation="ETGTKVYVLSNFSQETFEKIREHNAFLFDFDGMVISYQHHLIKP
                     DPRIYQLLIETYNINPARAVFIDDLEENVKAGREAGFFGIHLPPCADVTPYFIFPEGQ
                     ETVENSEFRQESK"
     gene            347..988
                     /gene="udk"
                     /locus_tag="PKV59_08025"
     CDS             347..988
                     /gene="udk"
                     /locus_tag="PKV59_08025"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HQP46734.1"
                     /translation="MNSDDESNSFHSITIGIAGGTGSGKTTFSQELVSRLNTDKIVYI
                     SHDSYYKDLSFMPLEERKKMNFDHPDSLDTDLMIQHLIALKEGKSVDIPVYDFKTFTR
                     TDQVEHITPQPVILVEGILIFAIKELRDLMDIKIFVDTDADIRFMRRLKRDIEERGRT
                     MESVYDQYLNEVRPMHEAFVEPSKRYADIIVPRGGRNDAALDMLAAKIRDYLS"
     gene            1437..>2172
                     /locus_tag="PKV59_08030"
     CDS             1437..>2172
                     /locus_tag="PKV59_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQP46735.1"
                     /translation="MDSITNSIKKRDHDPKISGRAKYVDDLRFEGMLYGKILRSTIAC
                     GIIKGIHIPDLPSGYTIVDKNDVPGVNIVHVVDDDTPVFSDGTVNYVGEPILMVVGED
                     QKVLKQILSEIIVDYEEAEPVLDMMKSETVFYHYGYTKGNPEKAFREADKIIDETFHT
                     GYQEHVYMEVQGMIGFYENGKSIVRGSMQCPYYVHTAVKKVMGAQTDQEVEVIQEEMG
                     GAFGGKEDYPSILGSQVAVAAKKVGRP"
BASE COUNT          691 a          393 c          493 g          595 t
ORIGIN      
        1 aagagaccgg caccaaagtc tatgttcttt ccaatttttc tcaggaaact tttgagaaga
       61 tcagagaaca caatgcattt ctgttcgact ttgacggaat ggtgatctct tatcagcatc
      121 atttgattaa accggatccg agaatttatc aattattaat cgaaacgtat aacatcaatc
      181 cggctcgggc ggtctttatt gatgatctgg aagaaaatgt aaaagccgga agagaagcgg
      241 gatttttcgg tattcatttg ccgccatgcg ctgatgtcac accctacttt atatttccgg
      301 aagggcaaga aacggtggaa aattcggagt tcagacagga atcaaaatga attcggatga
      361 cgaatcaaat tcttttcact caatcacgat cggaattgcc ggtgggaccg gttcggggaa
      421 aaccactttt tcacaggaac tggtctccag actgaacacc gataaaattg tttatatttc
      481 ccacgactcc tactacaaag atctttcgtt catgcctctg gaagagcgta agaaaatgaa
      541 ttttgatcat cccgattcct tagatacaga cctgatgatt caacatttaa ttgcactgaa
      601 agaaggaaaa agtgtggata ttccggtcta tgactttaaa acattcacac gtaccgatca
      661 agtggaacat attactcctc agccggttat tttggttgag ggaatattga tctttgcgat
      721 taaagaactc cgggatctga tggatatcaa gatttttgtc gatacggatg cggatattcg
      781 ttttatgcga cggttgaaac gggatatcga agagcgggga agaacgatgg aatcggttta
      841 tgaccaatat ctcaacgaag tacgcccgat gcatgaagcg tttgttgaac cttcgaaacg
      901 atatgccgat attatcgtcc cacgcggtgg cagaaatgat gcggcactgg atatgctggc
      961 tgctaaaatc agagattatc tttcctgagc cattccttca catgcgagaa ggaccgttcg
     1021 gcattctgct tttttagatt cattcgagat gctttttaac gattacggct tcgagaaacc
     1081 ctgatttttt acacattttt attacctttg tcagacaaat acaggtaaaa taccatatga
     1141 caaattattt gccatgtaga ataattctat taggcgaaat aagattgcaa ggagcgacct
     1201 cagccgattt tgattagttt aacggagaga aacagagtcg attgaacaaa tgcttggaca
     1261 aaaatggggt tgacttacgc ttaaaaattc ctttctgaat tcattcaaaa aggaacggta
     1321 actcttctaa atcaaaaaaa gatgtcagat tgacccgatt tagaaataat caaaggcaaa
     1381 agtaaaaaaa gatatagaaa ttgactttcg agtcagcgga taatatagga gcaagaatgg
     1441 attcaattac taattcgatc aaaaaaagag atcatgatcc gaaaatcagc ggcagggcaa
     1501 aatatgtgga tgacctacga tttgaaggca tgctttacgg caaaattttg cgatcaacca
     1561 tagcctgcgg aatcatcaaa ggaattcaca ttcccgattt accctccggt tataccattg
     1621 tggataaaaa tgatgtgccg ggcgtcaata ttgtccatgt ggtggatgat gatacaccgg
     1681 ttttttcaga cggtacggtc aattatgtgg gtgagccgat cttgatggtc gtcggcgaag
     1741 atcaaaaagt gctcaaacaa atattgagtg aaattattgt cgattatgaa gaagcggaac
     1801 cggttttgga tatgatgaaa tccgaaactg ttttctacca ttatggttat accaaaggaa
     1861 atccggaaaa agcctttcgg gaagcggata aaataattga tgaaactttc cataccggtt
     1921 atcaggaaca tgtttatatg gaagtccagg ggatgatcgg tttctatgaa aatggaaaaa
     1981 gcatagtgcg aggttcgatg caatgtccgt attatgtcca tactgcggtt aaaaaagtaa
     2041 tgggagcaca aaccgatcag gaagttgaag tcatacagga agaaatgggg ggagctttcg
     2101 gcggaaaaga ggattaccct tccattttgg gatcacaagt ggctgtagcg gcgaaaaaag
     2161 tcggccgtcc gg
//
LOCUS       DAOMIV010000365         2167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_57131_length_2167_cov_4.139205, whole genome shotgun sequence.
ACCESSION   DAOMIV010000365 DAOMIV010000000
VERSION     DAOMIV010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57131_length_2167_cov_4.139205"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PKV59_08035"
     CDS             complement(<1..704)
                     /locus_tag="PKV59_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46736.1"
                     /translation="MKKTLKKITGFQLFLPIVALILVLLFNMIKTPDFFKITVLNGTL
                     YGFMIDIINRASELAILAIGMTLVVASSAGTDISVGAVSAVTAAIICFSLGPDQQNYQ
                     MPYLIGILFGLLGGVLCGMWNGLLVAKMRIQPMVATLILFTAGRGISQLICSGQIIHV
                     EKESYRILGSFIPKVPLPTPVFVAAALALVTWLVLKKTALGMYIQAVGINRRAGRLIG
                     INSQLIIFMTYAYCGL"
     gene            complement(688..>2167)
                     /locus_tag="PKV59_08040"
     CDS             complement(688..>2167)
                     /locus_tag="PKV59_08040"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQP46737.1"
                     /translation="ALNNVDFTLRSGEIHALMGENGAGKSTLIKVLTGVEELEEGEIR
                     VSWLDHPLINHSPQEAQLNGISTVYQEVNLCPNLSVAENIFIGREPKKRGMIDWKAMN
                     DNSRKILRSLNLSQVDVTKSLEHYSIAIQQMVAIARAIDIESKILIFDEPTSSLEEQE
                     TEKLFQIMNQLKEKGVGIIFITHFLEQVYEVSDRITVLRNGELVGESLTKDLPRVQLV
                     AKMLGKEFNDLAAIKKETKEKVNHTKGVPLIDARNLGHRGTIKPFSLQMYPGEVVGLA
                     GLLGSGRSELARTIYGADKPDSGELFVNTKKVKINAPIDAMKERMGYLPEDRKEEGII
                     NDLSVKENIFIALQARQGMLKPLSNKEQTQLAEKYIKLLNIKVANIDSPVKQLSGGNQ
                     QKVILARWLSTQPDFLILDEPTRGIDVGTKTEIQKLVLNLAEEGMAIMFISSEIDEMI
                     RTVSRMIVLRDREKVGELSEEEMTQDHIMAAIAGGERDEEDA"
BASE COUNT          575 a          520 c          423 g          649 t
ORIGIN      
        1 caaagtccgc aatatgcata cgtcatgaaa atgatcagtt gggaattgat tccgatcagt
       61 cttccagctc tacgattgat acctacggct tgaatgtaca ttcccaaagc ggtctttttc
      121 aaaaccagcc aagtgacaag agccaaagca gcagcgacaa agacaggagt cggtaacggt
      181 actttcggaa tgaaactccc gaggatacgg taactttcct tttcgacatg aatgatctgt
      241 ccggaacaga ttaattggga tattccgcgg cccgcggtaa aaagaattaa tgtggcgacc
      301 atcggttgaa ttctcatttt tgcgaccaaa agaccgttcc acatcccgca cagaactccg
      361 cccaataatc cgaacaagat cccgattaaa tacggcatct gataattttg ttgatcgggt
      421 cctaaggaaa aacagataat cgcagcagta actgcgctaa ccgcacctac ggaaatatcg
      481 gtaccggcgg aagacgctac tactaaggtc atcccgattg cgagtatcgc caattcactg
      541 gctcgattaa tgatatcgat catgaatccg tacaatgtgc cgtttaaaac agtgatcttg
      601 aaaaaatcgg gggttttaat catgttaaac agcaaaacca ggattaaagc cacgatcggt
      661 aaaaacagct ggaaaccggt aattttctta agcgtcttct tcatctcttt cacctccggc
      721 gattgcagcc ataatatgat cttgagtcat ttcctcttcg ctgagttctc cgaccttttc
      781 tctatctctt aatacaatca ttcggctgac ggtacgaatc atttcatcaa tttccgaaga
      841 aataaacata atagccattc cttcttccgc cagattcaac actaattttt gaatttcggt
      901 tttcgttccg acatcaattc cccgtgtcgg ttcatctaaa atcaggaaat caggctgcgt
      961 tgacagccaa cgtgcaagaa tgactttctg ttgatttcct ccgctgagtt gcttaacggg
     1021 actgtcgata tttgcgactt tgatattcag caatttaata tatttttccg ccagttgcgt
     1081 ttgttctttg ttgctcaacg gttttaacat cccctggcga gcttgaagtg caataaatat
     1141 gttttctttt acggaaaggt cattgatgat cccttcttct ttgcgatctt ccggcaaata
     1201 ccccatccgt tctttcatcg catcgatcgg ggcgttgatt ttcacttttt tcgtattgac
     1261 aaataattct ccgctgtccg gtttgtctgc gccgtaaatt gtccgggcaa gttcggagcg
     1321 tcccgagccg agaagtcccg ctaaccccac aacttcaccg ggatacattt gtaagctgaa
     1381 cggtttaatc gttcctctgt gccccagatt tctcgcatcg atcagcggca cacctttcgt
     1441 atgattcacc ttctcttttg tctctttctt aatcgccgcc aaatcattaa attcttttcc
     1501 caacatttta gcgaccagct ggacacgcgg taaatctttc gttaaacttt cgccgactaa
     1561 ttcaccgttt cttaaaacgg taatccggtc ggatacctca taaacctgtt ccaaaaaatg
     1621 agtaataaaa atgattccca cccctttttc cttgagttga ttcatgattt gaaacaactt
     1681 ctcggtttct tgttcttcca atgaagaggt aggttcgtca aaaatcagaa tttttgattc
     1741 aatatcaatc gcccgggcaa tcgcaaccat ttgttggatg gcgatagaat aatgttccaa
     1801 ggatttcgtg acatcgactt gtgaaagatt gaggcttctg aggatttttc tggaattatc
     1861 attcattgcc ttccaatcga tcatgcctcg ctttttcggt tcgcgaccga taaaaatgtt
     1921 ttcggcaacc gaaagattgg gacataagtt cacttcctga taaacggtgc tgatcccgtt
     1981 gagttgcgct tcctgagggg aatgattgat taacggatga tccaaccaag agacacggat
     2041 ttcaccttct tcgagttctt cgacacccgt caaaactttg atcagggtag actttccggc
     2101 tccattttcg cccattaaag catggatttc tccgcttcgg agagtaaaat caacattatt
     2161 cagcgca
//
LOCUS       DAOMIV010000366         2162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_57345_length_2162_cov_3.171334, whole genome shotgun sequence.
ACCESSION   DAOMIV010000366 DAOMIV010000000
VERSION     DAOMIV010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57345_length_2162_cov_3.171334"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1865
                     /locus_tag="PKV59_08045"
     CDS             258..1865
                     /locus_tag="PKV59_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HQP46738.1"
                     /translation="MSYIPEKKTESLWWRDGVIYQIYPRSFMDTNGDGIGDLKGIIGK
                     LDYLSDLGIAAIWLSPVMPSPDADFGYDVANYKDIDPKFGTMDDYKNLIAEAHRRGIR
                     IIMDLVLNHTSTEHPWFLESKKSKDNPFHDYYLWQDHPNNWKSTFTQRTAWEYVESCG
                     QYYYHMFTVQQADLNWRNPEVYSEMMNIFRFWADLGTDGFRLDVFNLYFKDDQFRNNP
                     PAFHWIPFMRQKHIYDCDRPEMDRAVRNIRKVTNAYKDSYVVGETFLSSGEKIKRYCG
                     EDKLHGAFDFTFTHCKWDASAFRKIIKNVEQNSQEAGFWPTYVLNNHDVERSASRFGL
                     GEYDERLKEAAVLLILLRGTPYIYYGEEIGMRETKLKKSEMMDPVGVMWAPFNRGRDG
                     CRAPMQWNNQTNAGFTSGTPWNKVHPDYPERNVNAMVHDPNSLLTFYRQLIGLRKQFR
                     CLQVGNLELFDQKNESVLTFTRKFGKEIALIAINFTQFPQETDCKELGSEIIWTKSFS
                     NKSADISQPKDGILRLVGEQAVLLIGK"
     gene            1884..>2162
                     /locus_tag="PKV59_08050"
     CDS             1884..>2162
                     /locus_tag="PKV59_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46739.1"
                     /translation="MKKTKSWGVLLFLTFLFIAGRLDAYGILRDSVAANDTSSYFDCA
                     KADFTKIEAYECHRSATLPFIIQKLNPDLKYELTDVSEPFFGSEAELQV"
BASE COUNT          630 a          431 c          494 g          607 t
ORIGIN      
        1 tccaatcatc ccgataatag cccagtcgat tcatcccggg cagataaacc aacaatgtaa
       61 tcgcgaccag gatcaaacag atgagtaaat taccatgtcg taaggatttg cttttttcag
      121 ccatgaatcc ccctttgctt ctcttataga tgattttaat atgattataa aacaaacggt
      181 ttgcgatttt tgtccgagcc cgaatgttat aatttccaaa gagtcagaaa aaatcgattg
      241 aaaaaaggat gaatgcgatg agttatatcc cggaaaaaaa gacggaatca ctgtggtggc
      301 gggacggcgt aatttatcaa atttatcccc gttccttcat ggacacaaac ggagacggaa
      361 tcggtgattt aaaagggatt atcggtaaat tggattattt atccgatttg ggaattgcgg
      421 caatctggct ttcaccggtg atgccttctc ccgatgcaga ttttggctat gatgtcgcga
      481 attacaaaga tatcgatccg aaattcggaa cgatggacga ttataagaat ttaattgccg
      541 aagctcaccg gcgcgggatc cgaattatta tggatttggt tttgaaccat acatctaccg
      601 aacacccatg gtttcttgaa tcgaaaaaat cgaaggacaa tcctttccat gattattatc
      661 tctggcagga tcatcccaat aattggaaat cgacttttac ccaaagaacc gcctgggaat
      721 atgtcgaatc ctgcggtcaa tattactatc atatgttcac tgttcaacaa gcggatttga
      781 attggcgcaa tccggaagtt tactcggaaa tgatgaatat tttccgtttt tgggctgatt
      841 tgggaacgga cggttttcgg ctggatgtct tcaaccttta tttcaaagat gatcagtttc
      901 gaaacaatcc cccggctttt cattggattc cgttcatgcg gcaaaaacat atttatgatt
      961 gcgatcgccc cgaaatggat cgggcagttc ggaatattcg gaaagttaca aacgcttata
     1021 aagacagtta tgtcgtcggt gaaacgtttt tgagttccgg tgaaaagatt aagcggtatt
     1081 gcggtgaaga caaacttcac ggggcttttg atttcacatt tactcattgc aaatgggatg
     1141 cttcggcttt tcgaaaaatc attaaaaacg tcgaacaaaa ctcacaggaa gccggtttct
     1201 ggccgacata tgtcctgaac aatcatgatg tggaacgatc agcctccaga ttcggcttgg
     1261 gagaatatga cgaacggctc aaggaagccg ccgtcttact gatccttttg cggggaacgc
     1321 cttatattta ttacggcgaa gaaatcggta tgcgagagac caagcttaaa aaatccgaga
     1381 tgatggatcc ggtcggggtt atgtgggcgc catttaatcg gggacgggac ggttgtcgag
     1441 ctcccatgca atggaataat caaaccaatg ccgggtttac cagcgggaca ccttggaata
     1501 aagttcatcc ggattatcca gagcggaatg tgaatgcgat ggtccatgat ccgaattcac
     1561 tgttgacgtt ttacaggcaa ctgatcggat tgcggaaaca attccgctgt cttcaggtcg
     1621 gaaatttgga acttttcgat caaaaaaatg aatccgtcct gacatttacc agaaaatttg
     1681 ggaaagaaat cgctctgatt gcgattaatt tcacccaatt tccgcaagaa acagattgca
     1741 aagagttggg ttccgagata atttggacga aaagtttttc aaataaatcc gcggatattt
     1801 ctcaaccgaa agacggaatt ctccggctgg tcggggaaca agcggttttg ttaatcggca
     1861 aataaaagac gggaaaccga atgatgaaaa aaacgaaatc ctggggagtt ttactctttt
     1921 tgacttttct gtttatcgcc ggcaggttgg atgcttacgg cattttgagg gattcggtcg
     1981 ctgcgaatga tacctcttct tattttgatt gtgcaaaggc ggatttcacg aaaatcgaag
     2041 cgtatgaatg tcatcgttcg gctaccctac cttttattat tcaaaaactc aatccagatc
     2101 tcaaatatga actgaccgac gtttcggagc cttttttcgg atccgaagcg gaattacaag
     2161 tt
//
LOCUS       DAOMIV010000367         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_57369_length_2161_cov_5.252612, whole genome shotgun sequence.
ACCESSION   DAOMIV010000367 DAOMIV010000000
VERSION     DAOMIV010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57369_length_2161_cov_5.252612"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PKV59_08055"
     CDS             <1..192
                     /locus_tag="PKV59_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46740.1"
                     /translation="RHDGANPTRAGSPLALDSAIPSRLLSLRFSHRFQRIAKAQLIWC
                     PTSSDVSGIFLGIRLLMKH"
     gene            complement(322..819)
                     /locus_tag="PKV59_08060"
     CDS             complement(322..819)
                     /locus_tag="PKV59_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46741.1"
                     /translation="MSVAMEFTKEQLEQIGSYIESNFDRFSAKSNVVQLRPYEIQLIE
                     RMTRIEEELRSQRELILKQNETMQFGFAQMDKRFEQVDKRFEAMQKQMDERFESVQKQ
                     MDERFRAVDKRFEMIQKSMDNRFEQVDKRFDDVNKKFSMMMWMMTAGFTIIATVITLL
                     TIFAK"
     gene            1434..>2161
                     /locus_tag="PKV59_08065"
     CDS             1434..>2161
                     /locus_tag="PKV59_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HQP46742.1"
                     /translation="MITAVFDKTIFKPNESVKLSVTANCSGFFQLNSDGNENFDFEKD
                     TYRYSYSPNKTTDFILGGPEFTSFADMQLKPRSDNKYTHKTIYKLVLVFRDGLLQTSN
                     FPQDICVDGTGPKVDGFSPDIKDPFDFNPEIIITFDEDIGYADSSCLQIEPSDGKIKL
                     LRISKNQAFFSVTGLKALSDYSATVRGVKDIAGNVMEDSAPYSFKTISGDLQIKDVKG
                     NTNFAAYRGFEESSLNLKAYFKND"
BASE COUNT          609 a          432 c          449 g          671 t
ORIGIN      
        1 cgccacgatg gcgctaatcc aacgcgggca ggaagcccgt tagcgttgga cagtgcaatc
       61 ccatcccggc ttttgtcgct ccgattttcg caccgctttc agcggattgc gaaagctcag
      121 cttatttggt gtccgacttc gtcggacgtt tctgggattt ttctcggcat tcgtcttttg
      181 atgaagcatt aagattattg atatcatacc tctaccgggg aaagtcctta tttttggctt
      241 tgcttatttg tgatttgtgt tgggattttt gataaagttg cgaaagcccc tctttattaa
      301 agaggggttg gggtgattct cttactttgc gaaaattgtc agcagtgtta taacagttgc
      361 gataattgta aatcctgctg tcatcatcca catcatcatg gaaaactttt tatttacgtc
      421 atcgaatcgc ttgtcgacct gttcgaaacg gttgtccata cttttctgaa tcatttcgaa
      481 acgtttatca actgctctga acctttcatc catttgcttt tggacagact cgaagcgctc
      541 gtccatctgt ttctgcatgg cttcaaagcg tttgtcaacc tgttcaaagc gcttgtccat
      601 ctgggcaaat ccgaactgca ttgtttcgtt ctgtttaaga attaattcac gctggcttct
      661 gagttcttct tcgatgcggg tcattctttc tatcagctga atttcataag gacggagctg
      721 tacgacattg ctcttcgcgc tgaagcggtc gaagtttgat tcgatgtacg aacctatctg
      781 ttcaagttgt tctttcgtaa attccatagc aacactcatt ttcacctcat actattataa
      841 ataattcttt ctgtcaaata tatacgaaat ccagccctct tttttcataa aaaagaccgt
      901 aaaactcaaa aaaaagctgg aaaaaatgtc aatttttgtg ctgaatgaac actaaatatt
      961 gagagcttga aaaagttcaa aatttaaaga atgcttttga ggtcaaattg aggaaactgg
     1021 aaaagacttt atttataata tcatcggcat tatcattttt gataacctcg tgcggcggaa
     1081 gcgcaatcgg cgatgcgcag gaattttcac cgaacgaacc tccttctatt atatcaataa
     1141 aagccgtcaa cttcgacgga agcgaaataa cccagctcga tatcgagccg tataagcaat
     1201 ttaaactcat tgttgaagca attgatccgg ataacaatcc gcttgaatat aaattcgact
     1261 ctgaatcggg tactttctcg ggcatatctc ccaattcaac cggatgcacc gcaattttta
     1321 aaacaggaag ggttgtcggc ggacagaatg tcgaattctg ggcaggtgtt tctgacggaa
     1381 gaggcgcgct tgtccgtcag tcgtataatc tcggtacggg aaagctggga cctgtgataa
     1441 ctgcagtttt tgataagaca atatttaaac ctaatgagtc ggtaaagctt tccgttacag
     1501 ccaactgctc aggttttttt cagcttaaca gcgatggaaa tgaaaacttc gattttgaaa
     1561 aggatacgta ccgttattcg tacagtccga acaagacgac tgattttata ttgggcggac
     1621 ctgaatttac atcattcgca gacatgcagc ttaagccgag atcggataat aaatatactc
     1681 ataaaacgat ttataagctt gtattagttt tccgcgacgg attacttcag accagcaatt
     1741 ttcctcagga tatatgcgtg gacgggacag gacctaaggt tgatggtttt tcaccggata
     1801 tcaaagatcc ttttgatttt aatcctgaaa ttattataac ttttgatgaa gatataggat
     1861 acgcagacag ctcatgtttg cagattgaac cttctgatgg gaaaattaag cttttacgaa
     1921 tcagcaaaaa ccaggcattt ttttctgtca ccgggcttaa ggcattgagt gattacagcg
     1981 caacagtacg cggagttaag gacatcgccg gaaatgtaat ggaagacagt gctccttatt
     2041 catttaagac tatcagcggc gatcttcaaa ttaaggatgt taaaggaaat acaaattttg
     2101 cagcttaccg ggggttcgaa gaatcatctt taaatttaaa agcgtatttt aaaaatgatg
     2161 a
//
LOCUS       DAOMIV010000368         2159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_57451_length_2159_cov_3.765209, whole genome shotgun sequence.
ACCESSION   DAOMIV010000368 DAOMIV010000000
VERSION     DAOMIV010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57451_length_2159_cov_3.765209"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1298)
                     /locus_tag="PKV59_08070"
     CDS             complement(93..1298)
                     /locus_tag="PKV59_08070"
                     /inference="COORDINATES: protein motif:HMM:NF016104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HQP46743.1"
                     /translation="MSLKREPISVTAGFSPKRWLKENRSMVVSICQAWGITRLLLIFT
                     AWFSAYFPQNAVYQRYVDKGFQFTPIWPLDIWCRWDSAWYLSIAKNGYVPSGILSEQY
                     SNLAFFPLYPYLVRALTVWLPDFLRHQSVLISVGLLLSNVAFLLAMFGIYELARQLSD
                     ESTAQKAVMLTFCLPGAFFFGAFYTESLFLFLTVYAFLAAENERWVWAAVFSMLAALT
                     RANGILILVPILWIYMEKRQWQIRKIRRDLLWFLLAPAGVLLHFLNLYRITGNFFAFF
                     EAQSAWGRSVGGSLVSDFLRPLFRQPNQIAILDLVFITVYLLLSIVILIRYPRKAYGV
                     YALLTVLVLIRSGMLFSMTRYIAVIFPAVVYASLKIRNRKIFGSLCLFLIMLQILLYA
                     GWVNYYWIA"
     gene            1362..2108
                     /locus_tag="PKV59_08075"
     CDS             1362..2108
                     /locus_tag="PKV59_08075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQP46744.1"
                     /translation="MKITTVFFDLGNTLLYSDVPSPERVQAACKVMANEFAGRGYPVS
                     PEEFAAKHFENLLKYYDIREQENIEQGAESVFRQTLADFGFTGVPEKDIAAFLASFYR
                     TTQGNWHLDEDAIPTLERIIAQQRRIGLITNAGYAEDIRQLLRKNKLEPYFSSVVISA
                     EVGFRKPHPEIFQIALRQMGSAPEQSVMVGDSLLADIYGASRCGLRTVWFRKYTGDPP
                     DFSRFCHPDHELFSLRELPEILEKSETQPD"
BASE COUNT          640 a          593 c          493 g          433 t
ORIGIN      
        1 ggaaaaccga cccctacaat cattttcgca aacatgtggc accgtcccct acattatttc
       61 ctctgccaaa ggcgagggga attcaccttg gctcaggcga tccaataata attcacccag
      121 cccgcataca gcaaaatctg cagcataatc aggaagagac acagagatcc aaatatcttc
      181 cggtttcgga ttttcaacga ggcatacacg accgccggaa aaataacagc gatataacgc
      241 gtcatggaaa aaagcatccc cgatcgaatc aaaaccaaaa cagtcaacaa ggcatacacc
      301 ccataggctt ttcttgggta acggatcagg atcacaatcg ataaaaggag ataaaccgta
      361 ataaaaacaa gatccagaat tgcgatttga ttcggttgcc tgaacaacgg ccgcaagaaa
      421 tcgctcacca acgagccgcc cacggaccgc ccccaggcac tctgcgcctc aaaaaatgcg
      481 aagaaattgc cggtgatccg atacagattc aaaaaatgga gcagaacgcc tgccggagcc
      541 aacaaaaacc acagcagatc ccggcgaatc tttcgaattt gccattgccg tttctccata
      601 tagatccaaa gaatcggaac caaaatcagg ataccgtttg cccgtgtaag cgcagccaac
      661 atcgaaaaaa cagccgccca aacccaccgt tcattttcgg cagccaggaa agcataaacc
      721 gtcagaaaaa gaaacaaact ttcggtataa aaagctccga agaaaaaagc tcccggtaaa
      781 caaaaagtca gcataaccgc tttttgagcc gtcgattcat ccgaaagttg ccgcgccaac
      841 tcataaattc cgaacatcgc caacagaaac gccacattgg aaaggagcaa cccgaccgaa
      901 atcagcacac tctgatgccg cagaaaatcc ggcagccaaa ccgtcagcgc ccgaaccaaa
      961 tacgggtaca gcgggaagaa agccaaattg gaatactgtt cggacagaat cccggagggg
     1021 acataaccgt ttttggcaat ggaaagatac caagccgaat cccagcggca ccagatatcc
     1081 agcggccaaa tcggcgtaaa ctggaatccc ttatcgacat atcgttgata aaccgcattc
     1141 tggggaaaat aagcgctgaa ccaagcggtg aaaatcagca gcagcctggt aattccccat
     1201 gcttggcaga tcgaaacaac catcgatcgg ttctctttca gccaacgttt cggagaaaaa
     1261 ccggcagtta cggaaatcgg ttcccgcttg agcgacatgc tataatttta ccgtaatgaa
     1321 atcgaaaatc catcttttac gcaagtttcc cttcggatcc catgaaaatc accaccgtct
     1381 tttttgatct cggcaacacc ttattgtata gcgatgttcc gagtccggaa cgggtgcagg
     1441 ctgcctgcaa ggtgatggcg aatgaatttg ccggccgcgg ttatcctgtc tcccctgaag
     1501 aatttgctgc gaaacatttc gaaaaccttt tgaagtatta tgacatccgg gagcaagaaa
     1561 atattgaaca gggagcggaa tcggtatttc ggcagacatt ggctgatttc ggtttcaccg
     1621 gcgtcccgga aaaagacatc gctgctttcc tggcctcttt ctatcgaacc acccaaggaa
     1681 actggcattt ggacgaagac gccatcccga cgcttgaaag aatcatcgct caacagcgcc
     1741 ggatcggctt gatcaccaat gccggttacg ccgaagacat ccgtcagctg ctgcggaaga
     1801 ataaactgga gccctacttt tcgagcgtgg tcatatccgc cgaggtcggg tttcgcaaac
     1861 cccacccgga aatctttcaa atcgcgttgc ggcaaatggg cagcgcgccc gaacaaagcg
     1921 tcatggttgg cgactcgctg ctcgcggaca tctacggcgc atcccgttgc gggttgcgaa
     1981 ccgtttggtt cagaaaatac accggtgatc cgccggactt ttcccgtttc tgccatccgg
     2041 accatgagct gttctcgctt cgtgagctgc cggaaatcct cgaaaaatcg gaaacgcagc
     2101 cggattaatt ttcccccgct tgcggctggc aacacgggca aaagtgggtg ccccgctgg
//
LOCUS       DAOMIV010000369         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_57530_length_2157_cov_3.293530, whole genome shotgun sequence.
ACCESSION   DAOMIV010000369 DAOMIV010000000
VERSION     DAOMIV010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57530_length_2157_cov_3.293530"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            446..760
                     /locus_tag="PKV59_08080"
     CDS             446..760
                     /locus_tag="PKV59_08080"
                     /inference="COORDINATES: protein motif:HMM:NF041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CC/Se motif family (seleno)protein"
                     /protein_id="HQP46745.1"
                     /translation="MLKISPEAKAWLEKKGQSAVTLVRQVAKVNTCCCSTISYMDAVL
                     GKPQKDPAEYHKETIDGIDVYFQQKIKFPEDRDVTIELSNTLGLKSLKVTGMFQGGAI
                     TA"
     gene            851..2104
                     /locus_tag="PKV59_08085"
     CDS             851..2104
                     /locus_tag="PKV59_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46746.1"
                     /translation="MQKFQPKQLLREWLGIHEPLNDAEFVVGMRPIQTTDLPPLSIQE
                     VIAESQKAWRENPLAHRIISITTQYVIGKGVTFKACDSETQCFLRTFWDHPLNKMDLR
                     IHEWSDELSRSGNLFILISSDVSGMSYARAIPSARIREIRTRENDCEQAIAYKVIQPA
                     TDFLYDEKWIQAADLLNPTLEPVMIHFAVNRTIGSIWGEPDLAPLLKWLSRYANWLED
                     RVRLNRYRNSFLFVVRTHLAGEAQRLQRQSQLNAVPPVPGSILVTNENEEWSVLSPKL
                     ESMEAKEDGLALKKMIAAGAGIPLHFLAEPESENKASAESAGESTCRHFEQRQQLFLN
                     FLAELLQQVISRAALVNSRIRPEDSIRITGDDISVRDNLALSQAGVNIAEVLSGLKTQ
                     GLLSDDELLRITYRFIGEELQDVEK"
BASE COUNT          613 a          505 c          509 g          530 t
ORIGIN      
        1 ttttcaccct caccctgacc ctctccccaa gggcgaggga aatatttcgt aggggacgtc
       61 gtttttaaag cacaccgtag cggacggcgt cctcgacttc ccaagccgaa caaagtgagg
      121 cttttcctat agcccctcac cctgccctct cccaatgggc gaggacgtcc cgagccgaac
      181 aaagtgaggc attgcctaca gctcctcacc ctgaccctct ccccaagggc gagagaaaac
      241 tggatcgatg ggactgtgtc tttgcaaagc acaccagcgc ggatgcttcc cctacgccaa
      301 gacaattcga aaccaataaa cattactctt aatcattatt ggtaaatacc cccgatcatc
      361 tcctggtaaa ctttccccga ctattgacaa ccgatgtgga ttaaaattga acataatcca
      421 taataaacga aaaggaacta atcctatgct gaaaatcagt ccagaagcca aagcttggtt
      481 ggaaaagaaa ggacaatctg cagtaacgtt ggtaaggcag gtggcgaagg tcaacacctg
      541 ttgttgttcc acaatttctt atatggatgc agttttgggt aaacctcaga aagatccggc
      601 ggaatatcat aaagaaacaa ttgacgggat cgatgtctac ttccagcaaa agataaaatt
      661 cccggaagat cgtgatgtca cgatcgaatt aagcaatacc ctcggtttga aatctctgaa
      721 agttaccggc atgtttcaag gcggagcgat taccgcttaa tttatttttc agctcccttt
      781 attggcagta tggaaaccga ttcaaaacgg ctaccataaa aggaaagttc ttaaaataaa
      841 aggagctgtc gtgcaaaaat ttcaaccgaa acaactgctg cgggaatggc tggggattca
      901 tgaacccctg aatgatgcgg aattcgtcgt aggaatgcga ccgattcaaa caaccgatct
      961 tcctcccctg agtattcaag aagttatcgc agaatcacaa aaagcttggc gagaaaatcc
     1021 actggctcat cgaattatat cgattacgac acaatatgtg atcggaaaag gtgtcacgtt
     1081 caaagcctgt gattccgaaa cgcagtgttt tctgcggaca ttttgggatc atcccttgaa
     1141 taaaatggat ctgcgaattc acgaatggag cgacgaatta agccgcagcg ggaatctctt
     1201 tattctgatc tcgtccgacg taagcggcat gagctatgcg cgggcgattc cttccgctcg
     1261 gattcgtgaa atccggacgc gggaaaatga ctgtgaacag gcgatcgctt ataaagtgat
     1321 tcaaccggca acagattttc tgtacgacga aaaatggatt caggcagccg atctattaaa
     1381 tccgacctta gaaccggtca tgattcattt tgccgtaaac cggacgatcg gatcgatatg
     1441 gggagaaccg gatttggcac ctcttttgaa atggctgagt cgctatgcca attggttgga
     1501 ggatcgggtt cgcttgaatc gctatcgcaa cagttttctg ttcgtggtgc gtacccatct
     1561 ggcgggtgag gctcaacgtt tgcaacgtca aagtcagctg aatgctgtcc cgcccgttcc
     1621 gggttccatt ttggttacca atgaaaatga agaatggtct gtcctctctc cgaagctcga
     1681 gagtatggaa gcaaaagaag acggtttggc gctgaagaaa atgattgcgg ccggagcagg
     1741 aattccgctc cattttttag ccgaaccgga atcggaaaat aaagccagcg ccgaatcggc
     1801 gggagaatcg acctgtcgcc attttgaaca acgccaacaa cttttcctga attttctcgc
     1861 ggagctgctg caacaggtaa tttcccgagc tgcgctggtg aattcgcgca ttcgacccga
     1921 agattccatc cgaataaccg gtgacgatat cagcgtccga gacaacctcg ctttaagtca
     1981 agccggcgtg aatattgcgg aagtcctttc agggttaaaa acgcaggggc ttctttcgga
     2041 tgatgaactt ttgagaatta cctatcgatt tatcggagag gaattacaag atgtagaaaa
     2101 ataaatcatc gatttttcaa agatggcatg tcccttgcaa cttaaatcag tgtttgg
//
LOCUS       DAOMIV010000370         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_57613_length_2155_cov_2.772381, whole genome shotgun sequence.
ACCESSION   DAOMIV010000370 DAOMIV010000000
VERSION     DAOMIV010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57613_length_2155_cov_2.772381"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="PKV59_08090"
     CDS             complement(<1..467)
                     /locus_tag="PKV59_08090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQP46747.1"
                     /translation="MTRFILLRHGQTDWNIQRLYQGQEDIPLNETGKRQAEEAAETLK
                     NVPIDIAYCSDLQRAVETAKIVLRYHPSVPLMIDKRIRERSFGEYEGKPYQKDLMNPT
                     DSNEINKKRDSLNFKFPQGESLLDVYERANSFYNDILQKHPNEAVLIVSHGTF"
     gene            complement(488..>2155)
                     /locus_tag="PKV59_08095"
     CDS             complement(488..>2155)
                     /locus_tag="PKV59_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46748.1"
                     /translation="ICLLPLSGLWNSGISDQYIFGSSIPWSDAFTHHLNTLRFLYGQT
                     MGQSTAIRPLSVSFYSFILYITNNNFIILMSSLAVLSAFVMLINFRLIGIHIGPVAAA
                     FFFTNAFFFIRKFLGIYMTEPYAFIIGLLACYFLLNGIFKKQNGQILLGFCLFSLAMN
                     ARPGPMFTIPLIGLWYFFIFLKGHHRRIIYATVAFIALLSGFVINQWNVNRVYRSDKV
                     PNLQAAQIIYGLCLGGKDSYYVMGLPEINELNKSENIYKDVFGLCSNVLKEHPENIGV
                     SFNAIWNTLLFDSNRGSFSYFDGTENKMLIDLIRKGLMLLWSIGIFLIFRQRKQPFGS
                     FMIAMVLGLILSQFVVAPYTTFRMRYHAATIWVPGLIIGIFPQYVINRFAWKNLKRES
                     ESTPVTGSTSLALALSGFFILIALIAPELIGTRPLKPPAKPEISCKKGETTLYTKIDQ
                     GSYFYMENKSELQQEHYPYFRLPFVRQHFHDTASVEMFDFTDRIDQPTAIIRGIDLSD
                     WKDALIFSPLPLVKDRIGYVVFCGNYLDPPILRQDRFFIPSSAYFLQ"
BASE COUNT          698 a          428 c          427 g          602 t
ORIGIN      
        1 tgaaaagtgc cgtgtgagac gatcaaaact gcctcattcg ggtgtttttg taaaatatca
       61 ttgtaaaaac tatttgcccg ttcataaaca tctaacaaag attcaccctg cgggaatttg
      121 aaattcaaag aatccctctt tttgtttatt tcatttgaat cggtaggatt catcaaatcc
      181 ttttgataag gtttcccttc gtattcgccg aagcttctct cgcggattct cttatcaatc
      241 ataaggggaa ccgacggatg gtatcgcaaa acaattttcg ctgtttcaac cgcccgctgc
      301 agatcgctgc aataggcaat atcgatcggg acatttttta aggtttcagc agcttcttct
      361 gcttgtcgtt tccctgtctc attcaagggg atatcttcct gaccttgata aagtctttga
      421 atattccaat ctgtttgtcc atgccgtaat aaaatgaatc ttgtcatttg ttctgatcat
      481 tcctatttca ttgtaaaaaa tatgccgaag acgggataaa aaatcgatcc tgccgaagaa
      541 tgggcgggtc aagatagttg ccgcaaaata caacgtatcc gatacgatcc tttacgagcg
      601 gaagcggtga aaaaatcaat gcatctttcc aatcggataa atcaatgccg cgaataatcg
      661 ccgtaggctg atcaattcgg tcggtaaagt cgaacatctc aaccgaagcg gtatcatgaa
      721 aatgttgtcg aacaaagggc agcctgaaat agggatagtg ttcttgttga agctcggatt
      781 tgttttccat ataaaaatag ctgccctgat caatttttgt ataaagcgtt gtttcacctt
      841 tcttacaaga tatttcaggt ttggcaggtg gttttaaagg ccgcgtaccg attaattccg
      901 gcgcaatcaa agcgatgagg ataaaaaaac cggacagagc caaagccagt gaagtcgaac
      961 cggttaccgg ggtactttcg gactctcttt ttaaattttt ccatgcaaat cgattgatga
     1021 catactgcgg gaaaataccg atgattaaac cgggaaccca aattgtagcg gcatgatatc
     1081 tcattcgaaa ggtagtatag ggcgcaacca caaattgcga taatatcaag cccagaacca
     1141 tggcaatcat aaatgaacca aatggctgtt ttctctgccg aaatattaaa aatattccga
     1201 tcgaccacaa taacatcaat ccctttcgta tcaaatcaat cagcatttta ttttcagtac
     1261 cgtcgaagta agaaaatgaa ccgcgattcg aatcgaacag gagcgtattc caaattgcat
     1321 tgaatgagac cccgatattt tcaggatgtt ctttcaatac attcgaacaa agtccgaaaa
     1381 cgtctttata gatattctcg gatttgttta attcatttat ttccggtagt cccatcacat
     1441 aataggaatc tttccctccg aggcataatc cataaatgat ttgtgcagcc tgaaggttgg
     1501 gtactttatc ggatcgatag acccggttta cattccattg atttatgacg aatccgctta
     1561 ataatgctat gaaagcgacg gtcgcatata ttatccggcg atgatgacct ttcagaaaaa
     1621 taaaaaaata ccaaagccct atgagcggaa ttgtaaacat gggacccggg cgggcattca
     1681 tggctaatga aaaaaggcaa aatccgagaa gaatctgtcc gttttgtttc ttgaaaatcc
     1741 cgttcaataa aaaataacaa gcgagtaacc ctatgataaa tgcataaggt tccgtcatat
     1801 aaatacctaa aaactttctt ataaaaaaga atgcattcgt gaaaaagaaa gctgcggcaa
     1861 caggtccgat atgaatgccg attaatcgga aattaataag cattacgaaa gccgataaaa
     1921 cggctaaact gctcatcaag ataataaaat tattatttgt tatgtatagg ataaaactat
     1981 aaaaagaaac agaaagggga cgaatagccg tcgattgtcc catggtttgc ccatatagga
     2041 atcgtaaagt attcagatga tgagtaaagg catccgacca gggtattgaa ctgccgaata
     2101 tgtattgatc cgaaatgcca gaattccaca gacccgataa aggcagaaga caaat
//
LOCUS       DAOMIV010000371         2151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_57735_length_2151_cov_4.671279, whole genome shotgun sequence.
ACCESSION   DAOMIV010000371 DAOMIV010000000
VERSION     DAOMIV010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57735_length_2151_cov_4.671279"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PKV59_08100"
     CDS             <1..761
                     /locus_tag="PKV59_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46749.1"
                     /translation="QVEKGFYDDRKYLNAPLEIRAMLPEYKMVNIISSGDNKVLHNAI
                     FYNASRFILSEEGTFWYSDTPDDPGSKGWGNIMPRNCTWARLIEKESGQAFYFYNTHL
                     NHLSQRSRKKGVILLTGRISARTYPDAFVLTGDFNAREKSVPIQYLKGNTQINTKAEG
                     KVQNANPLTDTFRVRYPDYRNAATFHGYRRFFFRFKLDYIFVPSMVSVHDAQIIQLRQ
                     KRRYPSDHFPLYSHINFPESLAFAIPPVLIGGKA"
     gene            831..1667
                     /locus_tag="PKV59_08105"
     CDS             831..1667
                     /locus_tag="PKV59_08105"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQP46750.1"
                     /translation="MIPEIKNTIHVLRPVVIWRIFIKSIIFVLIPITFLHLTLIIASD
                     KVPAKQIAFQPGEKLIYKGKWGIIPAGEVTLEVLPKETIKGNEVYHFVMMTKTSAAVD
                     LVYKIRERQDSYVDIGMTHSIFYKKKTESPHPRNESINFNWEKGEATYTNFGQNKPPI
                     RILPGTFDPLALFYAIRLRTLKENSEIHLPLTDGNNLNIEVRAIIGKKDVIEIEGIMY
                     DTIEVTPDMGMLDELDKVVKKSDHPQLKIWVTADGKKIPVRIRSQVGILSFNFDLVPG
                     PL"
BASE COUNT          660 a          454 c          458 g          579 t
ORIGIN      
        1 agcaagttga aaaaggtttt tatgatgata gaaaatactt gaatgcgccc ttggaaatcc
       61 gcgccatgct tcccgaatat aagatggtca atattatcag ctcaggagac aacaaagtat
      121 tgcacaatgc cattttttat aatgcgtcac gtttcattct ttcagaagaa ggaacattct
      181 ggtattcgga cacccccgat gatccgggat caaaaggctg gggaaacatc atgccgcgca
      241 actgcacctg ggcgcgattg attgaaaagg aatccggaca ggctttttat ttttataata
      301 cccacttgaa tcatctgtca cagcgttcgc ggaagaaggg cgttatctta ttgaccgggc
      361 ggatatccgc gcggacttat ccggacgctt tcgttttaac cggtgatttc aatgcccgag
      421 aaaagagtgt cccgatacaa tatttgaaag ggaatacaca gatcaacacc aaagccgaag
      481 ggaaggttca aaacgccaat cctttaacgg acactttccg ggtaagatat cccgactatc
      541 gcaatgcagc cacctttcac ggctatcgcc ggtttttctt ccgtttcaag ctggattata
      601 tttttgttcc ttctatggtt agtgtgcatg atgcgcaaat cattcaactg cggcaaaaga
      661 ggcgttatcc ctctgaccat tttccgcttt attcgcatat taattttccg gaaagccttg
      721 cctttgcaat tcctcctgtt ctcatcgggg ggaaggcata aaagaacaaa gggattacct
      781 gacgggtgat cataaatgtt cttgtcaagt tcacgtgtaa aggtaaacaa atgattcccg
      841 aaatcaaaaa cacaatacat gtattgaggc cggtagtgat ttggcgtata tttatcaagt
      901 ccattatttt cgtattaatt cccattactt ttcttcattt gacccttatt attgccagcg
      961 ataaagttcc ggcaaagcaa atcgcttttc agccgggtga aaaactaatc tataaaggga
     1021 aatggggaat aattccagct ggagaagtca cactcgaagt tcttcctaaa gaaaccatca
     1081 agggcaatga agtctatcac tttgtcatga tgactaaaac tagcgcagca gtcgatcttg
     1141 tctataaaat tcgggagagg caggattcct atgtcgatat cggcatgacc catagcatct
     1201 tctataaaaa gaagacggaa agtccacatc ctcgcaatga aagcataaat tttaattggg
     1261 aaaaagggga agcaacttat acgaatttcg gtcaaaataa acctccgatt cgtatcttgc
     1321 cgggtacttt cgatccgttg gctctttttt acgctatccg tcttcggact ttgaaagaaa
     1381 attcagaaat tcatttaccg ctgactgatg gcaacaacct caacatcgaa gtcagagcaa
     1441 ttatcggtaa aaaagatgtg atcgaaattg aaggaataat gtatgacacc attgaagtta
     1501 cacctgatat gggaatgctg gatgaactgg acaaagtagt aaaaaaaagt gatcatcctc
     1561 agctcaaaat ctgggtaacc gctgatggaa aaaagatccc ggtaagaatc aggagtcaag
     1621 tgggaatcct ttcgttcaat tttgatcttg tgccgggtcc gttatgaagc aatataaaca
     1681 aaatttttca ccacggtcat gtttcctgcg atagccgagt tgcggtaatt gtccggcttc
     1741 ggtaaaaaag ccagagtgcg gcggccggcc tgcctcccga caaagtatcc ctcaatatac
     1801 tgaggtttca ctggacatgg agaccgactg aaccaatgaa tgcgttctga cctgtaatcc
     1861 taacagcccg ttattaaatc atttatcaaa tattctcatt caggatcgga gcttggtaac
     1921 atgttaataa cttataaaaa acattttcca gtctgtcaag gagttatgaa acacacatca
     1981 actcaatcag ggaagatact ttatggtcac ttctttcatg gtcaacctat gcagtgcgtg
     2041 ttttccggta gcggtagtat ctcagaggaa tcacgagaac gaaaaaggga atcatgtaca
     2101 tggcggcaac cagcggctcc acaggaccga aaaactgaga tgaaaaaaca a
//
LOCUS       DAOMIV010000372         2144 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_58018_length_2144_cov_3.485878, whole genome shotgun sequence.
ACCESSION   DAOMIV010000372 DAOMIV010000000
VERSION     DAOMIV010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58018_length_2144_cov_3.485878"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PKV59_08110"
     CDS             <1..310
                     /locus_tag="PKV59_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46751.1"
                     /translation="IIIMDGRRSTVGWAGRGDYVYPNVVMASGDMVAIDTEAIKILKQ
                     YPSRNRIQIPIDEMKTLTTAQKHGLGKMDYQIIEMEPHLSTREKNNNDPAAIAVMNDL
                     "
     gene            341..1156
                     /locus_tag="PKV59_08115"
     CDS             341..1156
                     /locus_tag="PKV59_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQP46752.1"
                     /translation="MLLKEKVALITGAGRGWGRSIAIAYARQGASVIAVARTKSELDQ
                     TAEIIRQEGGNVTEFAFDISEENAIKEIFQYISDHFSQLDILINNAAVLMLKPFTDLS
                     LDEIDQIIKINLRSQIVLCKMAIPQMIKQGGGSIINVSSGAGVKGFEKESIYCATKHA
                     IEGFSKSIALELMPYNIAVNTITPGGKSQNIQIKPTSLTQSEFELLSAEEKSKWTDSI
                     VSTDAFVFLGMQRADGITGQRILAYEFSQHLHECAWQLGLADLRDGLYETGKW"
     gene            complement(1232..1304)
                     /locus_tag="PKV59_08120"
     tRNA            complement(1232..1304)
                     /locus_tag="PKV59_08120"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1269..1271),aa:Asp,seq:gtc)
     gene            complement(1428..>2144)
                     /locus_tag="PKV59_08125"
     CDS             complement(1428..>2144)
                     /locus_tag="PKV59_08125"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQP46753.1"
                     /translation="NPADLLIFAVKYPALPQAIRDAKNQVGDKTIILSLMNGITSEQL
                     IGDTYGMDHMLYCVAQGMDATRTGNQLVYSSAGLLEFGELDNTLSEKVKAVKTLFKKA
                     DIQYRIPEDMNRIMWKKFMMNVGVNQVTAAYNSDYGAVQAKGEQRDTMIAAMREVIQI
                     ADRKEIHLTEEDIQYWLNVLDGFKPELMPSMRQDTAAKRKTEVELFSGAMIRMGKELD
                     VPVPVNEMLYKKIREIESAY"
BASE COUNT          623 a          442 c          466 g          613 t
ORIGIN      
        1 catcatcatc atggatggaa gaagatcaac tgtaggatgg gctgggcgcg gagattatgt
       61 ttatcccaat gtggtgatgg catcgggaga tatggtggcg attgataccg aggcaataaa
      121 aatattaaaa caatatccgt cgcgaaaccg tatacaaata ccgattgacg aaatgaagac
      181 actgactact gctcaaaaac atggtttggg aaaaatggat tatcagatca tcgaaatgga
      241 accgcattta tcaacaagag aaaagaataa taatgatccg gctgccatcg ctgttatgaa
      301 cgatctgtag aaagtacatt tatttatagg agaacggtaa atgttattga aagaaaaagt
      361 cgcattaatt accggcgccg gtcgcggatg gggaagatca attgcgatcg catatgcccg
      421 tcaaggtgca tcggttattg cggttgcacg aacaaaatcc gagcttgatc agaccgctga
      481 aattattcgt caagaaggtg gaaatgtcac cgaatttgct tttgatatca gcgaagaaaa
      541 cgcaatcaaa gagatatttc aatatataag cgatcatttt tctcagctcg atatcttgat
      601 caataatgca gcagtcctca tgttaaaacc ttttactgat ctttctttgg acgaaatcga
      661 tcaaattata aagatcaatc ttcgttcaca gattgttctg tgtaaaatgg cgataccaca
      721 gatgataaaa caaggagggg gaagcatcat taatgtttct tccggggccg gtgtgaaagg
      781 ttttgaaaag gaaagtatat attgcgccac gaaacatgcc atagaaggtt tttccaaatc
      841 aattgcatta gaacttatgc cgtataacat cgcagtaaat acaataactc cgggcggaaa
      901 aagtcaaaat attcaaatca aaccgacgag tttaactcaa tcggagtttg agcttctttc
      961 tgcggaggag aaatcaaaat ggacagattc cattgtatca acggatgctt tcgttttttt
     1021 ggggatgcag cgggctgacg gcatcaccgg acagagaatt ctggcatatg agttttcaca
     1081 acatttgcac gaatgtgctt ggcagcttgg tcttgctgat ttacgtgacg gattatatga
     1141 aacaggaaaa tggtgacctg taactgaatt aaatgcggta aataaaaaaa gatacaaacc
     1201 cactaatttg tatctttttt tatttttgtt gcgggagcga cggggttcga acccgcgatc
     1261 tcggccttga cagggccgca tgttagccgc tacaccacgc ccccatgagc gatccggctt
     1321 gaaagtgcct tcccaaactc aacggattgg attttaacac aggattgtaa gcaggtcaag
     1381 gcaagttctt tataatgttg ttattcaagc cgtctcttga tctccggtca atatgccgat
     1441 tcgatttccc gaatcttttt gtagagcatt tcgttgacgg ggaccggaac gtccagttct
     1501 ttgcccattc ggatcatcgc gcctgagaaa agttctactt ctgtctttct ttttgcggcg
     1561 gtatcctgtc gcatggaggg cattaattcc ggcttaaatc catccagtac atttaaccaa
     1621 tattgaatgt cttcttcggt taagtgaatt tccttccgat cggcgatttg aattacttcc
     1681 cgcatggcgg cgatcatcgt atccctttgt tcacccttcg cttgaaccgc cccataatcg
     1741 gaattgtaag cggcggtcac ttgattcact ccgacattca tcatgaactt tttccacata
     1801 attcgattca tatcctcggg aatgcggtat tgaatatcgg cttttttgaa aagagttttc
     1861 acagctttta ccttctcact caaggtatta tcgagttcac cgaattcaag caaacctgcc
     1921 gatgaataaa cgagctggtt tcccgttcga gtcgcatcca ttccctgcgc tacgcaataa
     1981 agcatatgat ccataccgta ggtatctccg atcaattgtt cactggttat tccgttcatc
     2041 aatgaaagaa taattgtttt atcccccact tgattttttg catcacgaat cgcctgaggc
     2101 agggcgggat atttgacggc aaaaattaat aaatcagccg gatt
//
LOCUS       DAOMIV010000373         2138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_58265_length_2138_cov_3.905425, whole genome shotgun sequence.
ACCESSION   DAOMIV010000373 DAOMIV010000000
VERSION     DAOMIV010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58265_length_2138_cov_3.905425"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..307
                     /locus_tag="PKV59_08130"
     CDS             29..307
                     /locus_tag="PKV59_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HQP46754.1"
                     /translation="MERVEYCEECQVGIPHFTTATYFTWIEDELITVPDFPCWVCDVC
                     GRREWDAESLMNLNLILSPNAGKPQPRKRTITGESKSTRAKTRRSAVR"
     gene            complement(376..1635)
                     /locus_tag="PKV59_08135"
     CDS             complement(376..1635)
                     /locus_tag="PKV59_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQP46755.1"
                     /translation="MYLKGSKYSYNGRKRRSNPFRVFFLFVLVAAGLYFSIYIAPETQ
                     PLFIPTNTPTTPPEAFISEAEQFAKEGRNAQAIELYQKAISSDPKNPSVYISLAKLQM
                     FETHYEDALKNAENAILLNGNNAVAHAIRGWALGKMGNYLDAETAFNRAAEIDPNNPL
                     TFAYRTEILVDQINAGQDSLNTLEKATEYSRLAIEHGNELMETHRARGLLLEITGNYE
                     DAINEFQIAISINPNISDLYLALGRNLRAAGKDSEAITAFERARSLNPYDSWAPYYIS
                     RTYSGNGEYAKAIQQAQDAIDLDPTNAMFYWNLGTQYYMSEDYANALTYFRLAIQGGT
                     TPNGEIIEGIPLSYDNRVPELYYMYGIILARSGNCQEAAEIVKLITQGIPNDTIAVEN
                     AQYMTDICKSGTFDITLTTPTAPAPAE"
     gene            1846..>2138
                     /locus_tag="PKV59_08140"
     CDS             1846..>2138
                     /locus_tag="PKV59_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46756.1"
                     /translation="MKKISIILALIAILVFCLPALADDVIKVCEVTDTGGVDDKSFNE
                     TAWNGILQAKEKYGIEAVVLESQSDADYAVNIDQFIEDECDLIVTVGFMLMDAT"
BASE COUNT          602 a          443 c          475 g          618 t
ORIGIN      
        1 gaccgcatca ctgaaaggag aatgaaatat ggaaagagtc gaatattgtg aagaatgcca
       61 ggttggtatt ccgcatttta ccacggcgac ttattttacc tggattgagg atgaattaat
      121 aaccgttcct gattttccct gttgggtatg cgatgtttgc ggtcgaagag aatgggatgc
      181 cgaatcgctg atgaatctta atttgatctt atcgccgaat gccgggaaac cgcaaccacg
      241 gaaacgaacc ataacaggag aatcaaagtc aacgcgcgcc aaaacgcgcc gaagtgccgt
      301 ccgttaaagc ggatgattga aataaaacga aaacataaaa aaagcttccg gcatatccgg
      361 aagctttttt tatgtttatt ctgccggcgc cggtgcggtc ggcgtcgtca atgtgatatc
      421 aaaggtccct gatttgcaga tatcggtcat gtattgagcg ttttcgacgg cgatcgtatc
      481 attgggaatg ccttgtgtaa tcaatttgac gatttccgcc gcttcctgac aattcccgct
      541 ccgggcgaga ataatgccgt acatataata cagctccgga acacggttat cgtatgagag
      601 aggaattcct tcgataattt cgccgttggg agtggtcccg ccctgaattg ccaacctgaa
      661 atacgtcaaa gcattggcat agtcttcgga catataatat tgcgttccga gattccaata
      721 aaacatggca ttggtcggat ctaagtcgat tgcatcctga gcctgctgaa tcgctttggc
      781 atattcaccg ttcccgctat acgttcggga aatatagtaa ggtgcccagg aatcataagg
      841 attcagtgat cgtgcccttt caaatgcggt gatcgcttcc gaatcttttc cggcggctcg
      901 cagatttctg cccagagcaa ggtaaagatc cgatatattc ggattgatag aaatcgcaat
      961 ttgaaattca ttaatcgcgt cctcatagtt accggttatt tccagtaata aaccgcgtgc
     1021 ccgatgcgtc tccatcaact cgttcccatg ttcgatcgcc agtcgtgaat attcggtcgc
     1081 tttttcgagt gtattcaaac tatcctgtcc cgcattgatc tgatcgacga gaatttccgt
     1141 tcgatatgca aaagtcaacg ggttattggg gtcgatttcg gcggctcgat tgaacgcggt
     1201 ttcagcatcc aaataattcc ccattttgcc gagcgcccaa ccgcgaatcg catgggcaac
     1261 ggcattattc ccgtttagca gaatcgcatt ttccgcattt tttaatgcat cttcataatg
     1321 ggtctcgaac atctgaagtt tcgcgagact gatataaacg gaaggatttt tcggatcgga
     1381 actgatcgct ttctgataga gttcaattgc ttgggcattt cgcccttctt tcgcaaattg
     1441 ttccgcttcg gaaataaaag cttcgggagg ggtagtcggc gtattggttg gaataaacag
     1501 cggctgtgtt tcgggcgcaa tataaatact gaaatataat ccggcagcta ccaaaacaaa
     1561 aaggaaaaat actctgaatg gatttgatct cctttttctt ccgttatagc tgtatttcga
     1621 tccttttaaa tacacacttt cagccctttc tcaaatcatt tcaatcttta gaacttatta
     1681 taatcgtccg agtgttcgga aaaaaagatt ctgattcaat ttaaataatt gtatttttca
     1741 ggaattaata ttatttttgt cagacaaaaa aggtggaaat tttaatggga aacctttata
     1801 ataaaagaag gtaagtatat caacccaaaa taaaaggaga aaaaaatgaa aaaaataagt
     1861 attatcttgg cattaattgc aatccttgtg ttttgcttgc ctgccctggc ggatgatgtg
     1921 atcaaagttt gtgaagttac tgataccggc ggtgtagatg ataaatcttt taacgaaaca
     1981 gcttggaacg gtattctcca agcaaaagaa aagtacggaa ttgaagccgt cgttctggaa
     2041 tcacaatccg atgccgatta tgccgtcaac atcgatcaat tcatcgaaga cgaatgcgat
     2101 ttaatcgtta ctgtcggttt catgctgatg gatgcgac
//
LOCUS       DAOMIV010000374         2137 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_58320_length_2137_cov_2.982709, whole genome shotgun sequence.
ACCESSION   DAOMIV010000374 DAOMIV010000000
VERSION     DAOMIV010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58320_length_2137_cov_2.982709"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PKV59_08145"
     CDS             <1..399
                     /locus_tag="PKV59_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP46757.1"
                     /translation="AAKKDIQILTEGETSIISGIPRLVFEIVYNLVDNAIKYNREKGT
                     VRISVKTDRQFVMLRVSDTGIGISKEDQARVFERFYRVDRSHNRDSGGTGLGLSIVKH
                     AAMVHHAKVELSSQINVGTTITVSFPVRQS"
     gene            615..>2137
                     /locus_tag="PKV59_08150"
     CDS             615..>2137
                     /locus_tag="PKV59_08150"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQP46758.1"
                     /translation="MTEKKWVYLFSEVDQAEAYCGGDWDKVRGLLGGKGANLADMARI
                     GVPVPPGFTVTTEACNYYSAHGKFPEGLWEQMLAALAEVEKAAGKKFGDQKNPLLVSC
                     RSGAKFSMPGMMDTVLNIGLNDETALGMVELTGNERFVYDSYRRLLQMFGSVVLEIRD
                     EAFEKPLEEYRAKKGYKLDTEMTAEDWKALCETYKKIVRVETGHDFPQDPYEQMRLAT
                     VAVFNSWNGKRAVDYRRAEKIPDDLGTAVNIVTMVFGNMGEDSGTGVAFTRNPLDGTK
                     KIYGEYLLNAQGEDVVAGIRNTQPLETLQTVMPDVYTQFVDIASRLEKHYRDMQDMEF
                     TIERGKLWMLQTRNGKRTARAEVAIAVQMVNEGLIDKETALKRVTTKQVDALLHPQFD
                     PEALKKIEPFATGVNASPGAAVGQIYFDADTVVEMKETENKDCIMVRQFTKPDDVHGM
                     LSAKGILTSEGGATSHAAVVARQFGVPCVVGASSLKIDLERKTVTAGDLVLHEGDWIS
                     "
BASE COUNT          603 a          453 c          555 g          526 t
ORIGIN      
        1 gcggcgaaga aagacattca gatccttacg gaaggcgaga cgagcatcat ttcgggaatt
       61 ccgcgattgg tctttgagat cgtctacaac ttggttgaca acgcgatcaa atataatcgg
      121 gaaaaaggaa ccgtccgcat ttcggtaaag acggaccgcc aatttgtcat gttacgggta
      181 agcgacaccg gcatcggcat ttccaaagaa gatcaagctc gagtctttga acgcttctat
      241 cgggtagatc ggagtcacaa tcgagactcc ggtggaacgg ggctgggact ctctatcgta
      301 aagcatgccg cgatggtgca tcatgcgaaa gtagaattat ccagtcaaat aaatgtcgga
      361 acgacaatta ccgtttcttt tcccgttcga caatcctgac aaaccttccc gaatcagacg
      421 ctatgacata taaaaatgcc ttaagatatt tttaaactta tcggttacgt catctctatc
      481 ataaaaattt attttatttt catataagta agcattttga cgggtactga tgcccatatg
      541 gtaaaattta tttacaatat accaggattt gataaaaatt aatgcggaaa tatttttagg
      601 aggaagaaag tccaatgact gagaaaaaat gggtctattt attcagcgag gttgatcaag
      661 ctgaagctta ttgcgggggg gattgggaca aggtccgcgg tctgctgggt ggaaaagggg
      721 ccaatcttgc agacatggcg cgaatcggtg tacccgttcc gccggggttt acagtaacaa
      781 ctgaagcctg taattattat tctgcccacg gaaaattccc cgaaggtctt tgggagcaaa
      841 tgctggctgc gttagccgaa gttgaaaaag cagccgggaa gaaattcgga gatcagaaaa
      901 atcccctttt ggtatcctgt cgttccggcg ccaaattttc tatgcccggg atgatggaca
      961 cggttttgaa catcggtttg aatgatgaaa ccgcgcttgg aatggttgaa ttaaccggta
     1021 acgaacggtt tgtatatgac tcttacagac gtctcctcca aatgtttggt tctgtggttc
     1081 ttgaaatccg cgatgaagct ttcgaaaagc cattggaaga atatcgagcc aaaaaagggt
     1141 acaaactgga taccgaaatg acggcagagg attggaaagc cctttgcgaa acctataaga
     1201 agattgtcag ggtcgaaacc ggtcatgatt tcccgcagga tccctacgaa caaatgcgcc
     1261 ttgccacggt tgccgttttc aattcatgga acgggaaacg agcggtggat taccgtcgcg
     1321 cggaaaagat tccggatgat ttgggaacag ccgtcaatat cgtcactatg gtattcggga
     1381 atatgggtga agattccgga accggcgttg cttttacacg gaatccgttg gacggtacca
     1441 agaagattta cggcgaatat ctgttaaatg ctcaaggtga agatgtcgta gccggaatcc
     1501 gaaatacaca gccattggaa acactacaaa cggttatgcc ggatgtttac acccagtttg
     1561 tggatatcgc cagccggttg gaaaaacact accgggatat gcaagatatg gaatttacca
     1621 tcgaacgcgg taaactctgg atgctgcaga ctcggaacgg aaaacggacc gcgcgggcgg
     1681 aagttgccat tgcggtacag atggtaaacg aagggttgat cgataaagaa acggctttga
     1741 agcgggttac gacgaaacag gtcgatgctc tgctgcatcc ccaattcgat cccgaagccc
     1801 tcaagaagat cgaaccgttt gccacgggcg tcaatgcctc tcccggcgcg gcggtcggac
     1861 agatttattt tgatgccgat accgtcgtcg aaatgaaaga aaccgaaaac aaagattgca
     1921 ttatggttcg gcaatttact aagccggatg acgttcacgg tatgctctcc gcaaaaggta
     1981 ttttaacctc tgaaggcgga gccacttccc atgcggcagt tgttgcccga cagttcggtg
     2041 ttccctgtgt ggtcggcgct tcttcgctga aaatcgattt ggaaagaaaa acggtaactg
     2101 ccggggatct ggtgctgcat gaaggcgatt ggatttc
//
LOCUS       DAOMIV010000375         2124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_58842_length_2124_cov_3.331078, whole genome shotgun sequence.
ACCESSION   DAOMIV010000375 DAOMIV010000000
VERSION     DAOMIV010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58842_length_2124_cov_3.331078"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1195
                     /locus_tag="PKV59_08155"
     CDS             278..1195
                     /locus_tag="PKV59_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HQP46759.1"
                     /translation="MPFLIIQQDITKIKTDAIVNAANTALRMGGGVCGAIFRAAGAAE
                     LQAACDKLAPIQTGDAVITPGFRLPAKYIIHTAGPIYRDGKHGEAMQLRSCYSNSLKL
                     ALKNGCKSIAFPLISAGIYGYPKKEALSEATSAIRDFLAENEMEIYLAVLDKSSIVVD
                     ENLLKNLSEYIDRVYEKPSGISNEGEGDSVKRNVEPFLEILTQIVKKSGLAEIEVCTK
                     ANLSRDWFSKVGSKGGSFVTKQTIIALSVALKIPFEETVELLAQTGFNLSRNQLFDVI
                     VSYFMRNQLYDIDLINEVLFLYDQQLLGS"
     gene            complement(1336..1758)
                     /locus_tag="PKV59_08160"
     CDS             complement(1336..1758)
                     /locus_tag="PKV59_08160"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HQP46760.1"
                     /translation="MLNNESLTEPSPSQKIDAQINELGEDWRGQKLKEIRQLIKQTDP
                     EIVEEWKWRGTAVWSHNGIICTGEVYKNVVKMTFAKGALLPDPDHLFNSGLEGNIRRA
                     IDFHQDDEINKIALSQLIRDAITVNDSSRKSVHKSELI"
BASE COUNT          654 a          383 c          446 g          641 t
ORIGIN      
        1 aaaattcagg ttgtgtatag taccagacat gaaataggaa tatccaaaaa aatgtttcga
       61 aaaggtggaa gagattattc actataatta ataggccatt cttttaggat aggatcgaaa
      121 gaaaaggaat ttcgcataga tattgtttta tcttagaaac ggattcattc gctatcagaa
      181 aggcaatatt tcggtttgat ttgagggaat ccagttaatc aatcgataaa gccggcagta
      241 aaaaaatgag agtcaatcgg atcaaggaag gaaaattatg ccgtttctga taattcagca
      301 agatataaca aaaataaaaa cagacgcaat cgtcaatgcg gcaaataccg cccttcggat
      361 gggaggcggt gtttgcgggg caatttttcg ggcggccgga gcggctgagc tacaagctgc
      421 ttgtgataaa ttagctccga ttcaaaccgg agatgccgtt atcacaccgg ggttcagact
      481 tcccgctaag tatattatcc ataccgccgg tccgatttac cgggacggaa aacatggtga
      541 ggcgatgcag ctccgctcgt gttactctaa cagcctgaaa ttggctttga aaaacggatg
      601 taagtccatc gcatttccgt tgatatctgc tgggatatat ggctatccca aaaaggaggc
      661 attgagtgaa gcgacctctg ctattcggga ttttttggct gagaacgaga tggagattta
      721 tttggctgtt ttggacaaat cttccattgt agtcgatgag aacctattaa aaaacctatc
      781 tgagtatatc gatcgggtgt atgaaaagcc gagtggaata tcaaacgaag gggaaggcga
      841 ttcagtaaag aggaatgtgg aaccgttttt ggaaatcctt acacaaatag ttaaaaagag
      901 cggtttagca gagatagagg tttgtacaaa ggccaatctc agccgggatt ggttttctaa
      961 agtcggaagc aaaggtggat cttttgtgac aaaacaaacg attattgcac tgtctgttgc
     1021 gttaaagatt ccattcgaag agacggtaga acttttggct caaaccggct ttaacttatc
     1081 gcgaaatcag ctgtttgatg tgattgtaag ttattttatg cgaaatcaac tatatgatat
     1141 cgacctgatt aatgaagtgc tatttcttta tgatcagcaa ttgttgggga gttgacagga
     1201 aagtttaaat atttcggctg ataaataaag gattgtcgat catactcacg gattgataga
     1261 tatagggcaa aaaatcagag aaaaaaatta tatgtttgaa gcgaaagcta aacgatattt
     1321 tctggttacc ggagcttaaa tcaattcgga tttgtgaacg cttttacggg aagaatcgtt
     1381 tacagtaatc gcatcccgaa ttaattgtga aagtgcgatt ttgtttattt catcatcttg
     1441 atggaaatcg atcgctcttc tgatatttcc ttccaaacca gaattgaaca aatgatcggg
     1501 atccggcaag agcgctcctt tcgcaaaggt cattttgacg acatttttat agacttcacc
     1561 ggtacagatg atcccattgt gcgaccatac ggctgtacct ctccatttcc attcttcaac
     1621 tatttcggga tctgtctgtt tgatcaattg acgaatttct ttcaattttt gacctcgcca
     1681 atcctcaccg agttcattga tttgagcatc tattttttga ctgggggaag gctctgtcag
     1741 cgattcgttg tttaacatcg attattcctc taatcttttc tcttttatac cggaaaccgg
     1801 aaggattgtc tccgccgatt aatttcttgt caataatcaa tttctatata aatctcttcg
     1861 aaaaattctt atctaaaaat gatgaatgca aaaaatagca aatccaagga aaaaattcat
     1921 atcgattacc gatttgttta ataattcagc cgccgaagat ctaagtaaaa agctcgagaa
     1981 tatgcttttt aacctcttgt ccattttatt cggcggctca ttgataacag caattaattt
     2041 tccgctccga ttttcaaaaa tccagcctac ctatgttatt cagttccata aacttccgat
     2101 tgctatggac ctaaaaatca atat
//
LOCUS       DAOMIV010000376         2117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_59124_length_2117_cov_4.603783, whole genome shotgun sequence.
ACCESSION   DAOMIV010000376 DAOMIV010000000
VERSION     DAOMIV010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59124_length_2117_cov_4.603783"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..889
                     /locus_tag="PKV59_08165"
     CDS             482..889
                     /locus_tag="PKV59_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQP46761.1"
                     /translation="MALEVGQIVEGKVTGITNFGAFIELPTGQTGLVHISEVADEYVK
                     DVNQFLKQNDLVKVKVLNLDKGKIGLSIRQAQPSSSAHSERSRMGPKAPSPKTFEDKL
                     AKFMKDSDERLLDLRKNTDSKRGGRGGGRAPSM"
     gene            complement(1075..1884)
                     /gene="folP"
                     /locus_tag="PKV59_08170"
     CDS             complement(1075..1884)
                     /gene="folP"
                     /locus_tag="PKV59_08170"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQP46762.1"
                     /translation="MKISTPKILGIVNITDDSFSDGGTYLDTGKAMEKSLELIGNGAA
                     IIDLGAASSNPASKKINPQEEINRLKPVVDFLLSKNISISIDSFQPEVQKYALQSQVR
                     YLNDIQGFPYTEIYPDLAESACKLIVMHSIQRLGPATVIETKPDKIFNEIIDFFSERI
                     KILQNSGIALDRIILDPGMGFFLGSNPESSIQVMKNISRFKEHFNLPILVSVSRKSFL
                     GNITGRKISERGPATLAAEIYLSYMGVDYIRTHDARSLNDALKILTTLNSN"
BASE COUNT          626 a          400 c          473 g          618 t
ORIGIN      
        1 ggttctatgt catgaatcat gttcccgaga tcttcggata tctgaagacc atgaaatacg
       61 gcgaccgcag cgcgatcgat atcccggaag aaagcttcat gtagggaacg gtcgtgaccg
      121 tccctctgct gtaaggtccc tcccttgatg ggagggattc agggagggtg aagatgtagc
      181 gaacggtttt taaccgttcc gggactgtag taaaaaaatc gtcattcccg cgaaggcggg
      241 aatctaggaa aatgataaaa aggctgaagc gtaacgcgct tcagcctttt atcttatcgg
      301 aaaagtataa tgggaacctc ccatttatta tttatccgca ggatttcttg tgactgcgcc
      361 actgtcaggt ccctccctgg atgggaggga ttcagggagg gtgacacttt tgggcatacg
      421 ttataatata ctatgtcaag gtcttttaag accttaattt aagaggagga attactggag
      481 aatggcatta gaggttggcc agatcgtgga aggaaaagta accgggatta ctaattttgg
      541 agcttttatt gaactgccta ccggtcaaac cgggttagtt catatttccg aggttgcgga
      601 tgagtatgtt aaagacgtga atcaattttt aaaacaaaat gatctggtca aagttaaggt
      661 tttaaacctg gacaaaggca agatcggtct ttcgatccgc caagcgcaac catcatcgtc
      721 agcccattcg gaacggagta gaatgggtcc caaggcacca agcccgaaga cgtttgagga
      781 taagttagcc aagtttatga aggattccga tgagcggctg ttggatctcc gtaaaaatac
      841 ggattccaaa agaggtggcc gtggtggcgg ccgggctccg agtatgtaat gatataagat
      901 ataagatata agataattta aacggttata atccggtcat gaaccggatt ttatttttcc
      961 ggtaaattat cagtactccg gtaccgattg aatgtttgag catcgccgta acatgacaat
     1021 atagtgaatc aatggtcgat tcattatttc ttcaaaggtt atctgcatga ttattcagtt
     1081 tgaatttaaa gttgtcagta ttttgagcgc atcattcaaa gaccttgcat catgagtacg
     1141 gatatagtcc acacccatgt aactcaaata aatttccgcc gccagggtgg ctggcccccg
     1201 ctcactgatt ttacgcccgg tgatgtttcc taaaaacgat ttccgggata cggaaaccag
     1261 tattggtaaa ttaaaatgtt ctttgaatcg agagatattt ttcatgacct gaattgaaga
     1321 ttccggattg gagcctaaaa agaaccccat tcccggatcc aaaatgatcc gatctaaggc
     1381 aatgcctgaa ttttgcagta tcttaatgcg ctctgaaaag aaatcaatga tttcattgaa
     1441 tattttatca ggtttggttt cgataaccgt ggccggccct aaacgctgga tagaatgcat
     1501 aacaatgagt ttgcaagccg attccgccaa gtcggggtaa atttcagtat atggaaatcc
     1561 ttgaatatca tttaaatacc gaacttgact ttgtaaagcg tatttttgaa cctcaggttg
     1621 gaaagaatcg attgagattg aaatattttt ggaaagcaag aaatcaacta ccggttttaa
     1681 ccggttgatt tcttcttgag gattaatttt tttcgacgcg ggattactgg atgcggctcc
     1741 caggtcaata atcgcggcgc cgtttccgat cagttcaagg gatttttcca tggctttacc
     1801 ggtatcgaga taggttccgc catcagaaaa actatcatcg gtaatattta caattcctaa
     1861 tattttaggt gtggatatct tcatcggcat agtccttttg acgatattat tcgacttgta
     1921 atatgtctaa ttaataactt taatagctta ctccttctcc ttaaacacca tagacgccaa
     1981 aatacttagc gccaaaatac caaaaataac cataatagaa gttttaatgc caatatcgat
     2041 attaaagtat agaatcgaca gcttaattaa ttgaattgat aattgaattg ataagaactt
     2101 gcttttagtt gacggca
//
LOCUS       DAOMIV010000377         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_59363_length_2111_cov_3.800097, whole genome shotgun sequence.
ACCESSION   DAOMIV010000377 DAOMIV010000000
VERSION     DAOMIV010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59363_length_2111_cov_3.800097"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="PKV59_08175"
     CDS             <1..378
                     /locus_tag="PKV59_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46763.1"
                     /translation="EIAEAEELPKPVSFEKSERKYEGKAEDFVDLTQLPLQTETTPAE
                     PEPQTEYEDISFDELFNAMRNEIATPTEYLEDEDELKDDLTSTKGKKKKKKKNVEIEY
                     DPDSDSTLIHKKHKRGEDEDWGW"
     gene            379..678
                     /locus_tag="PKV59_08180"
     CDS             379..678
                     /locus_tag="PKV59_08180"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HQP46764.1"
                     /translation="MSNKSEKKHVRHVPQRTCIACRQTLPKRSLIRLVRTDEGLQLDP
                     TGKVSGRGAYLHDSKACWNIAIQHRLLEKSLKTELSDKDLEKMRLWMEDLPDNAE"
     gene            724..>2111
                     /gene="infB"
                     /locus_tag="PKV59_08185"
     CDS             724..>2111
                     /gene="infB"
                     /locus_tag="PKV59_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQP46765.1"
                     /translation="MGDEAKRVQIPYTITVRDLAKLLQSNSIQVMRILMANGVMANIN
                     QLVDFDTAAIVASEMGFEAVPESKEQVEEFKETGDVPLWRQIINKEDPKSLIPRPPVV
                     TILGHVDHGKTSLLDAIRHTNVAVGEAGGITQHNGAYQIENKGKLITFLDTPGHAAFS
                     AMRARGAQGADIVVLVVAANDGVMPQTKEAISHAKAAQVPIIVALNKIDRSDANPDLV
                     KKQLSEQGLIPDEWGGNTMVVPVSAKQRIGIDDLMEAILLTAENSEILANPKGKVFGT
                     VIEASIDPKKGVMATLLVQNGTLKMGDVVLAGQSIGKIRAMFDFRGKKIQKAGPSTPV
                     QIMGLTEVPTAGDIFQVYASEKEARAVVENAKNEAALKSSTPKATLEELFAKFKAGEA
                     KELNLIIKTDVNGSLEPIINSINDLSKEGEIKINILHAEAGNISENDVMLASASHGVV
                     IGFNVQADPVAK"
BASE COUNT          646 a          420 c          560 g          485 t
ORIGIN      
        1 gaaattgccg aagcggaaga gttgcccaaa ccggtttctt ttgaaaaatc agaacgtaaa
       61 tatgagggaa aagcggaaga ttttgtcgat ctgactcaac ttcctcttca aacggagacc
      121 acgccggctg aacccgaacc tcagaccgaa tatgaagata tttcttttga tgagcttttc
      181 aatgcgatgc gaaatgaaat cgcgactcct acggaatatt tagaggatga agacgagctg
      241 aaagacgatc tgacttcaac taaaggcaaa aagaagaaga agaagaaaaa tgtcgaaatc
      301 gaatatgatc ctgacagcga ttcgacattg atccataaaa agcataaacg cggggaagat
      361 gaggattggg gttggtaagt gagcaacaag tctgaaaaga aacatgtcag gcatgttccg
      421 caacggacgt gcatcgcttg tcgacaaacc ttgccgaagc gatcgttgat ccgattggtc
      481 agaacagatg aaggcttgca gcttgatccg accggaaagg tctccggtcg gggagcatac
      541 ctgcatgatt cgaaagcatg ttggaacatt gcgatccaac acaggctgct tgaaaagagc
      601 ctgaagactg aattgagtga taaagattta gagaagatga gattatggat ggaagatctt
      661 ccggataacg ctgaatagca aacatacttg aaggtcttct gccataacgg aaagagagtg
      721 gctatgggtg atgaagcaaa aagagtacag ataccttata ctataaccgt ccgtgatttg
      781 gcgaagctac ttcagagtaa cagcattcag gtaatgagaa ttctgatggc caacggggtc
      841 atggctaata ttaatcagct ggtggacttc gatacggcag caatcgttgc cagtgaaatg
      901 ggttttgagg ctgtacccga atccaaagaa caggtagagg aattcaaaga aaccggagat
      961 gttccgctct ggcggcagat cataaataaa gaagatccga aaagtttgat tccgcgtccg
     1021 ccggtcgtga cgattttagg acatgtcgat cacggcaaga cgtctttatt ggacgccatc
     1081 cgtcatacaa atgtcgccgt gggggaagcc gggggaataa cccagcataa tggtgcctat
     1141 cagattgaaa ataaagggaa attaattaca tttctggaca ctccggggca tgcggcgttc
     1201 agtgcaatgc gcgcacgcgg tgctcaggga gcggatattg ttgtgcttgt tgtggctgcc
     1261 aatgatggtg tcatgcctca gacgaaagag gcaatttcac acgccaaagc cgcacaggtt
     1321 ccgattattg ttgcgctgaa taaaatcgat cgctccgatg ccaacccgga tttggtaaaa
     1381 aagcagcttt ccgaacaagg cttgataccg gatgaatggg gcggaaacac gatggttgtt
     1441 ccggtctccg ccaagcagcg aatcggtatc gatgatttga tggaagcgat tctgttgaca
     1501 gccgaaaaca gcgaaatttt ggctaatccc aaaggaaaag tattcggaac ggttatcgaa
     1561 gcttccattg atccgaagaa gggcgttatg gcaacccttt tggttcaaaa cggcactttg
     1621 aaaatgggtg atgttgtttt agcgggtcag tcgatcggta aaatcagggc gatgtttgat
     1681 tttcgcggca agaagattca aaaggccggt ccctcgacac cggttcagat tatgggtctg
     1741 accgaagtac cgacggctgg agatattttc caagtctatg cgtcggaaaa agaagctcgg
     1801 gcggtggttg aaaatgcgaa aaatgaagcg gcgttaaaaa gctcgacacc gaaagccaca
     1861 ttggaagaac tgtttgccaa gttcaaagcc ggtgaagcaa aagaattgaa cttgattatt
     1921 aagacggatg tcaacgggtc tttggaaccc attatcaatt ccatcaatga cctcagcaaa
     1981 gaaggcgaaa ttaagatcaa tattctgcat gccgaggctg gcaatatcag cgaaaatgat
     2041 gtcatgcttg catctgcatc tcatggagtt gtcatcggat ttaatgtcca agccgacccg
     2101 gttgcgaaga a
//
LOCUS       DAOMIV010000378         2110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_59413_length_2110_cov_2.667153, whole genome shotgun sequence.
ACCESSION   DAOMIV010000378 DAOMIV010000000
VERSION     DAOMIV010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59413_length_2110_cov_2.667153"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..587
                     /locus_tag="PKV59_08190"
     CDS             318..587
                     /locus_tag="PKV59_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46766.1"
                     /translation="MSVTLKLEDLREIFREMAGSLKSVPKDPPPEEFLSRKEVLSILK
                     IDSSTLWSWEKTGYIKSFPFGGRKRYKSVDVEAIRTGRKGVRHGR"
     gene            577..1767
                     /locus_tag="PKV59_08195"
     CDS             577..1767
                     /locus_tag="PKV59_08195"
                     /inference="COORDINATES: protein motif:HMM:NF040238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HQP46767.1"
                     /translation="MEDKEKYIRVGTTLYKIVRRPLISGDYIEEKILWSYEALRQDYG
                     KNNLPEIEKYDGFCIIPSHIDYQQVYGTFLNQYEPVNHTPCEGDFPYILLFLEHIFEE
                     QIELGLDYIQLLYTRPTQILPILLLVSNERETGKTTFLRFLKIIFGKNATFNTNEDFK
                     SQFNADWANRLLVLVDELLLNKMEDTEKIKNLSTAGDYKIEAKGKDRREIEFFAKFVL
                     CSNNEKNPIIIPKEEIRFWVRKVKPVEKDITSLRELMTKEIPCFLYFLMNRKLSTQNE
                     SRMWFKPSLLETPALNKIKKYNSSKVEIEMASYCAEVMERLELNTFHCCPKDFLDIMR
                     NAGLKTDISQIRSILKDSWGLRSDRNSDYTFYWVGFDGEIHPMKRKGRYLEIEKGLVD
                     KILL"
BASE COUNT          713 a          386 c          418 g          593 t
ORIGIN      
        1 tggcttcagc aagttttcat cgatttgcaa gatcttgaat attcctatgt aaaaggcaaa
       61 cttcacgact atcaaaccaa gaaacatcat acccaatatt gacgatattc ccaaatattt
      121 agcggaacac aataagtaaa aacattcatt ttcggagata atggagagat tttcgagaga
      181 ttccaagtga tttgaatctc ttaaatatct ctctttttat ttgccccgga ttcaattgag
      241 tccggaagca aattattgta taactataaa aatttccgat tatgaactat cgtgatttat
      301 tgaattccgg ggcgaatgtg agtgtaacac tcaaactgga agatctgcgg gaaatcttta
      361 gagagatggc gggcagtctt aagtccgtac ccaaagaccc accacccgaa gagttcctaa
      421 gccgtaaaga ggttttatct attcttaaaa ttgattcttc tactttgtgg agttgggaga
      481 aaacaggtta tatcaaatca tttccatttg gaggacgaaa gcggtataaa tctgtagatg
      541 tcgaagcaat acgcaccggc aggaaaggag tacgtcatgg aagataaaga aaaatatatc
      601 cgtgtcggga caaccttgta taaaattgtt cggcgtccgc tgatcagtgg agattatatt
      661 gaagagaaaa tcctctggag ttatgaagcg ctgcgtcaag attatggaaa gaataatctc
      721 cccgaaattg aaaaatacga tggcttttgc attattccga gccatataga ctaccaacag
      781 gtttatggaa cttttcttaa ccaatatgag cctgtcaatc atacgccttg cgagggtgat
      841 tttccttata tcctgctatt tctggaacat atttttgaag aacagataga gttggggttg
      901 gattatattc aacttttata tacccggcca acacagatac ttcccattct tttactggtt
      961 tccaatgaaa gggaaacagg caaaacaacg ttcctgcgat ttttgaaaat aattttcgga
     1021 aagaatgcca cattcaatac gaatgaagat ttcaaaagtc agttcaatgc cgattgggca
     1081 aaccgcttgt tggttctggt cgatgaactt cttctgaata agatggaaga caccgaaaag
     1141 ataaaaaact tatcaaccgc tggcgactat aaaatcgagg ccaagggaaa agatcgccgg
     1201 gagattgaat tctttgccaa attcgttctt tgttccaata acgaaaagaa tccgattatt
     1261 attccgaaag aagaaattcg tttttgggtt cgcaaagtca aaccggttga aaaagatata
     1321 acttctctaa gagagttgat gacaaaagaa attccctgtt tcctgtattt tcttatgaac
     1381 cggaagctat ctacccaaaa cgaatcccgt atgtggttta aaccctcatt actggaaacc
     1441 cccgccttga ataaaatcaa gaagtacaat tcgagtaaag ttgagattga aatggcttcc
     1501 tattgtgccg aagttatgga acgcctcgaa ctaaacacat ttcattgttg tccgaaagac
     1561 tttttggata ttatgcggaa tgccggactg aaaacggata tttctcaaat ccgaagcatt
     1621 ttgaaagata gttgggggct tcgttctgac cgtaacagcg actatacttt ttattgggtt
     1681 ggttttgacg gggagataca tccaatgaaa agaaaaggac ggtatttgga gatagaaaaa
     1741 ggtttggtgg ataaaatcct gttgtaatgt tttcttctta tcgctaatta gctaacaatc
     1801 aaaaagaaca taatagcaac aaaaccgcaa caatcataca acaaaatcga aattgtcggt
     1861 aaccatcaac aacgaaaacg tcgtttgttg tgtatatgtt gagcctataa tttagtggaa
     1921 tactgtagaa tatcccaacc ctacaacaaa tcaacaaaaa tagaaatatg caagcagaca
     1981 atataaaaaa catacccatc cgggattacc tgaaaaatca gggaacttat cctgcaaaag
     2041 aatattccgg ttacggaatg tataaaagtc cgttccgtga cgaacgcacg ccgagtttca
     2101 aagtcgatta
//
LOCUS       DAOMIV010000379         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_59629_length_2105_cov_2.999024, whole genome shotgun sequence.
ACCESSION   DAOMIV010000379 DAOMIV010000000
VERSION     DAOMIV010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59629_length_2105_cov_2.999024"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..>2105
                     /locus_tag="PKV59_08200"
     CDS             348..>2105
                     /locus_tag="PKV59_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46768.1"
                     /translation="MQKNDPIQKKRRLIIPALVAILILVLIFQTSLENPHHFIMGPYK
                     AFALSFLLSVFFLSLDFCFCQRSRSVLNAWFFFFAGFILLGIFSLSQYLFPNSNRLAG
                     LGRWSFFPVIICLFGIGLRLNLFFSLRKSNTVSVSQTFLIIFAVFFLFLSLLPILSGG
                     FYWDDAIFATVIPAMRISGVSIWQRIKDEIISYSLRGRINPFATFQFLIFYLFPDVHV
                     YKAILLILTIIDGLLFYKFNSELFKNQIAALAILLLLPLCIQLRIYHDPMQGYYGLMQ
                     MMFAELMISLICFNRYLENRKKKYLIISIIFFTIGLMSYEMFYPLLVLYFLLAWHSQK
                     NRKQTLSPCIPFALVETILLSVSFGFRSYSAKMGVGAYSGTTFSLDIGKILSTWFHQS
                     LAALPLSYQMAGNDATILKRLIPASEIFSQSFAAFVRGIVWSDIVSLIVFLFLWVEIR
                     KRIHLPVISGFHWAFGCTLLFGSGLVLALSEKYQNEILPGLGYLPVFFGYFGAAFMIF
                     CLAANVYRRLKKTISANTILTFGISVFMVVFLLNQQSNRRSVALMNEEFLYPRQTGEA
                     ALQAGILKETNPKKEILVEN"
BASE COUNT          534 a          407 c          422 g          742 t
ORIGIN      
        1 cggaagtgaa agaatttata gataaccttt tcaagactta tcccgataac aatttccaaa
       61 taaaaattat gatacagcta tatcggattt caccgctttt ctatttgaaa tcttccgaaa
      121 tcggaaatgc cgtatttaat aaataacaaa caaataataa ccttctacag aaaaattaaa
      181 tgattaagac gaaaccgatg caaatttttg gatccttttg ccaacatacc ggatgtagat
      241 atttatttcg gcgaaatatg agaaaaacct gatttgagtg ctgttattgc ggcatttttc
      301 attacgagaa atatacaatt ggaaatcatt catttataat acctgacatg caaaagaatg
      361 atccgataca aaaaaaaagg cgtttgatta ttccggcgct ggtcgcaatt ctgatcttgg
      421 tattaatttt ccaaacttcg ttggaaaacc cacatcattt tataatgggc ccgtacaaag
      481 cttttgcttt aagctttttg ttaagcgtct tttttctttc gcttgatttt tgtttctgtc
      541 agcgaagtcg ttctgtttta aatgcttggt tctttttctt tgcaggcttc atcctattag
      601 gcatattttc tctttcgcaa tatctttttc cgaattcgaa tcgcttagcc ggactcggaa
      661 gatggagctt tttccctgtc attatttgtc tgttcggcat cggtttacgg ttgaatcttt
      721 ttttctcgct ccgaaaaagc aatacggttt ctgtaagcca aacgtttttg atcattttcg
      781 ccgttttttt tctgttcctc agtttgcttc cgattctttc cggtggtttt tactgggatg
      841 atgcgatttt tgctacggtg attccggcaa tgcgaatttc gggagtgtcg atatggcaac
      901 gtatcaagga tgaaattatc agctactcgt tacgagggcg tatcaatcct ttcgctacct
      961 ttcaattttt aattttttac ctgtttccgg atgttcatgt ctataaagcg atattattga
     1021 ttctaacaat aatagatggc ttattatttt ataaattcaa ttcggaatta ttcaagaatc
     1081 aaatcgccgc tttagcgatc ttgctgctct taccgctctg tattcaactt cgaatttatc
     1141 atgatccgat gcagggctac tatggactga tgcaaatgat gttcgcggaa ttaatgatct
     1201 ccttaatctg ttttaaccgc tatttggaaa atcggaaaaa gaaatatctg atcataagta
     1261 tcattttttt tacgattggt ttgatgtcct atgaaatgtt ttatcccctt cttgtgttgt
     1321 attttttgct ggcttggcat tcccaaaaga accggaaaca aactttgtca ccctgcattc
     1381 cttttgcatt agtcgaaacg attctcttgt cggtttcatt cggcttccgt tcctattcgg
     1441 cgaaaatggg agtgggtgct tactcgggga caacattcag tttggatatc ggaaaaatct
     1501 tatctacctg gtttcaccag tctcttgccg cgcttccgct cagttatcaa atggcgggga
     1561 atgatgcgac gatattaaag cgcttaattc cggcatccga aattttcagt cagtcttttg
     1621 cggcatttgt ccgcggcatt gtttggtcgg atattgtttc gttaatagta tttttatttt
     1681 tatgggttga gattcgcaaa cggatccatt tgccggttat ctccggattt cattgggcat
     1741 tcggctgtac gctcttgttc ggatccggtc tggtattggc tttgagcgag aaataccaga
     1801 atgaaatact tcccggctta gggtatttgc cggttttctt tggctatttt ggcgccgcct
     1861 ttatgatctt ttgcttagct gccaacgtat atcggcgcct gaaaaaaacg atctcagcga
     1921 atacgattct gactttcggg atatccgttt ttatggtggt atttctgctg aatcagcaat
     1981 caaaccggcg gtcggtggct ctgatgaatg aggaattctt atatccccgg cagacgggtg
     2041 aggcagcttt acaagccgga attctgaaag agacgaatcc gaaaaaagaa attctggtgg
     2101 agaac
//
LOCUS       DAOMIV010000380         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_60083_length_2094_cov_4.048063, whole genome shotgun sequence.
ACCESSION   DAOMIV010000380 DAOMIV010000000
VERSION     DAOMIV010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60083_length_2094_cov_4.048063"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1200
                     /locus_tag="PKV59_08205"
     CDS             52..1200
                     /locus_tag="PKV59_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQP46769.1"
                     /translation="MGPQHPALKEPGHFEFTVDGEIVVGASIRLGYVHRGVEKAAEQR
                     TYTQDLYLLERVCGICSHSHGTAFIQCVEKLAGVELPPRAKAVRCIAGEIERLHSHLL
                     WLGVAAHEGGFDALFMYSWRDREIVMDILENFFGNRVNYSVNIPGGVKEDITQEFIDG
                     VTPKLDYLEKRMYHYLDVVNTDMTFRQRTVDIGTIGLEESDRLGATGPTARASGVARD
                     IRIDRPYDGYDLFPIDITLDNRGDLNARFVVRIKECIASIHYIKNVMNHMPEGELSVK
                     IPRKIPTGEYMASVEAPRGELFYFVRSNGTESPDRVKIRTASLCNWGTIVSSVAKGHK
                     LADMPMILAGIDPCFSCNDRMVYINKGSGNPEIWTWEQLRKYGIAYYK"
     gene            1197..>2094
                     /locus_tag="PKV59_08210"
     CDS             1197..>2094
                     /locus_tag="PKV59_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQP46770.1"
                     /translation="MSAPHPLICLFFFPGGLFLILAALLYEWADRKLVARFQNRVGPR
                     VFQPVADMFKLLVKEEVIPKSVNRSLFIVLPIISFACVMTASLYVPMFGVEAPIGLHG
                     DLIITMYLLSVMTMCLGMAGANSMNRFAVVGATRTFTQLFAYEAPFMLALLMPAISAQ
                     SWNINVISEYASAHTWMIATIPIGFIISVIGLMGKLELAPFDAPEAESEIVSGALSEY
                     SGRGLALFRIAKDVEFVIGISLIVTFYLGGFGNLLSFLLRSLGLLVIIALLQSSMTRL
                     RIDQTVGFWWQAGAILSLIQLMI"
BASE COUNT          564 a          427 c          494 g          609 t
ORIGIN      
        1 gaaacagtaa acaatttcaa taacaatggt cagcaaaagt atattgttcc tatcggaccg
       61 caacatccgg cattgaagga accgggacat tttgaattca ccgtagacgg ggaaattgtc
      121 gtaggagctt ctatccgttt aggttacgtt catcggggag ttgaaaaagc tgccgaacaa
      181 cggacttata ctcaggattt atatctgtta gaacgcgttt gcggtatttg ttcgcattcc
      241 cacggcaccg cttttattca atgtgtggag aaactcgcag gtgtcgagct gccgccgcga
      301 gcgaaagcgg tgcgctgcat agcaggggag attgaacgtc tccatagtca tctgttatgg
      361 ctgggcgttg ccgctcatga gggtggattt gatgcgttgt ttatgtattc atggcgagat
      421 cgagaaatcg tgatggatat tttggaaaac ttttttggga accgtgtaaa ttactcggta
      481 aatatccccg gaggagtcaa agaagatatc acacaggaat ttattgacgg agtaactccg
      541 aaattagatt accttgaaaa aagaatgtac cattatctcg acgtcgtgaa tacagatatg
      601 acatttcgac aacgaacggt ggatatcgga acaattggtt tggaagaatc cgatcgtctg
      661 ggtgcgacag gaccgaccgc acgagcatcc ggagtagctc gagatatccg catcgatcgt
      721 ccttatgatg gttatgattt atttcccatc gatataactt tagataaccg gggagattta
      781 aacgctcgat ttgtagtaag aataaaagaa tgtatcgcat ccattcacta catcaagaat
      841 gtgatgaatc atatgccgga aggtgaactg agcgtgaaaa ttcctcgaaa gattccgacc
      901 ggtgaatata tggcatcggt tgaagctccg cgaggcgagt tattttattt cgtccgatca
      961 aacggcacag aaagtccgga tcgggtaaaa attcgaacag cgagtctatg taattgggga
     1021 acaatcgttt cttcggtggc aaaaggtcat aaactggccg atatgccgat gatccttgca
     1081 ggaatcgatc cttgcttttc ctgtaacgac cgaatggttt atattaataa aggctcaggg
     1141 aatccggaaa tttggacctg ggaacaatta cgaaaatatg ggatagcata ctacaaatga
     1201 gcgcaccaca tcctcttatt tgtcttttct tttttcctgg cggattattt ctgatattgg
     1261 ccgctttatt atacgaatgg gctgacagga aattagtcgc aagattccaa aatcgagtcg
     1321 gtccgagagt atttcaaccg gtcgccgata tgttcaaatt actggtaaaa gaagaggtaa
     1381 tccccaaatc cgtaaacaga tcattgttca ttgtcttgcc gattatctct ttcgcatgtg
     1441 tcatgaccgc atcactttac gtccccatgt tcggagtcga agcaccgatc ggtttgcatg
     1501 gagatttaat tatcacaatg tatcttttgt cggtcatgac catgtgttta gggatggccg
     1561 gtgcaaactc aatgaatcga tttgcggtcg tcggcgcgac cagaacattt acacagttat
     1621 ttgcttatga agcaccgttc atgcttgcgc tcttgatgcc tgccatttcg gctcaatctt
     1681 ggaatatcaa tgtgatttca gaatacgctt cagcccatac ctggatgatc gcaaccatac
     1741 cgattggatt cattatttcg gttatcggct taatgggaaa attggaattg gcaccttttg
     1801 atgcacccga agctgaatcg gaaatagttt cgggagcttt atccgagtat tccggtcgag
     1861 gtttggcatt attccgcatc gcgaaagatg ttgaattcgt tataggcatt tctttgatcg
     1921 tcacattcta cttgggcgga ttcggaaatt tgctgtcatt tttattacga tcactcggat
     1981 tgcttgtaat cattgctctc ctgcaatcct ctatgacccg cctccgcata gatcaaacgg
     2041 taggtttctg gtggcaagca ggtgctatct tatcacttat ccaacttatg atat
//
LOCUS       DAOMIV010000381         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_60123_length_2093_cov_4.056428, whole genome shotgun sequence.
ACCESSION   DAOMIV010000381 DAOMIV010000000
VERSION     DAOMIV010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60123_length_2093_cov_4.056428"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PKV59_08215"
     CDS             complement(<1..210)
                     /locus_tag="PKV59_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46771.1"
                     /translation="MRSLNKIITTILSAIVACNAVSIPVFSQNGQTDPIPVEVNQTGS
                     ASAEFLCRPDNYSMNQDYCVSLGPSE"
     gene            387..1115
                     /locus_tag="PKV59_08220"
     CDS             387..1115
                     /locus_tag="PKV59_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HQP46772.1"
                     /translation="MKTTIVIPTYNEYENLPLLVERIFALPMDNLHLLIVDDNSPDGT
                     GKLADELAVRYDNRIKCMHRSGKLGLGTAYNQGFRMAMADGADYIGQMDADFSHPIDK
                     IPVMAAALKDCDVVIGSRYVKGGKLDESWPFYRKWLSGFGNFYARAILGLSIRDVTGG
                     FKLWKRETLEAMPLDRIKANGYMFQVEMNYVAAKLGFKFVEIPIYFRERMAGTSKMNL
                     AISREAATRTWALRRMYKDLKPIK"
     gene            1186..2046
                     /locus_tag="PKV59_08225"
     CDS             1186..2046
                     /locus_tag="PKV59_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HQP46773.1"
                     /translation="MLVTLKDVLEIAEKRTCAIGGFNGPNLASILGVLSAAEELNAPV
                     ILMHAQSHESYMPLEVVGPILLREAERSSVPVCVHLDHGVDIPYLEKALDLGFTSVMY
                     DGSTLPFEENIANTQKVIRLAAAKGASVEAEIGVMHGAEGSVDHDEDPASVYTDPESA
                     KEFVERTGIDALACSFGTVHGIYLKEPKLDFDRVAKIRKLTGKPIVMHGGSGVSHDDY
                     RKVIARGVRKINYYTYMARAGADNVKKHLAEAKGTVLFEHIESWAMEAIHDDVKKTIS
                     VFWESQTPVV"
BASE COUNT          595 a          404 c          523 g          571 t
ORIGIN      
        1 ttccgaagga cccaatgaaa cacaataatc ctgattcatg gagtagttgt ccggccggca
       61 taaaaattcg gcagacgcac tgcctgtttg attcacttcg acgggaatcg gatctgtctg
      121 tccattctgt gaaaaaacag gaattgaaac ggcgttgcat gccacaatcg ccgatagtat
      181 ggttgtaatt attttattta aagaccgcat aactttcctt cgaacacctt ctgccttatt
      241 tatttcctct tgtttgagcg gcagaccctt tcttggatcg tccgctcatc cataaattct
      301 acgatataat aatcgaaaaa taattataat cgcagagtga tcccgatttt gtcttttcga
      361 acagaatgaa agacagacaa ggaaaaatga aaacgaccat tgtgattccc acatataacg
      421 aatacgaaaa tctgcctctc ttggttgaaa ggatttttgc ccttcccatg gacaatcttc
      481 atttgctgat tgtcgatgat aacagccctg atggaaccgg aaaattggcg gatgaactgg
      541 ctgtccgata tgacaatcga atcaaatgta tgcatcgatc gggaaagctc gggctgggca
      601 ccgcttataa tcagggattt cgtatggcga tggcggatgg ggcggattac atcggtcaaa
      661 tggatgccga tttctctcat ccgattgata agatacctgt tatggcggca gcattaaaag
      721 attgtgatgt ggtaatcggt tcccgttatg tcaaaggcgg aaaactggat gaaagctggc
      781 ctttctatcg aaaatggttg tccggtttcg ggaactttta tgcgcgcgcg attctgggct
      841 tatccattcg ggatgttacg ggaggattta aactttggaa gcgagaaaca cttgaggcaa
      901 tgccgttgga taggattaaa gcgaacggat acatgtttca ggtggagatg aattatgtcg
      961 ccgcaaaatt gggttttaaa tttgtagaaa ttccgattta ttttcgggaa agaatggcgg
     1021 gaacttccaa gatgaatttg gcaatttccc gtgaagcggc aacccgaacc tgggctttac
     1081 gcagaatgta taaggacctg aaacccatta aataaattct gtaaaataat aacataatta
     1141 agaccgataa aattattctg ataaatgaaa ttcgaggatt aatccatgct ggtaacgttg
     1201 aaagatgtat tggaaatcgc tgaaaagcgg acatgtgcga ttggcgggtt caacggaccg
     1261 aatcttgcaa gtattttggg ggttctctcg gctgccgaag agctgaatgc tccggtcatt
     1321 ttaatgcatg cccaatccca tgaaagctat atgccattgg aagtagtggg accgatttta
     1381 ctccgtgagg ctgaacgatc atctgttccc gtctgtgttc atctggatca tggggtggat
     1441 attccctatt tggaaaaggc gcttgatctc ggattcacgt cggtcatgta tgatggttcc
     1501 actctgcctt ttgaggaaaa tattgcgaat acgcaaaaag tgattcgatt ggcagccgct
     1561 aaaggtgctt cagtcgaagc cgaaatcggc gtcatgcacg gagcggaggg gtcggtggat
     1621 cacgatgaag acccggcatc cgtttatacg gatccggaat cggcaaaaga gtttgtcgaa
     1681 cgaaccggaa ttgacgcttt ggcttgttcg tttgggacgg ttcacggaat ttatttaaaa
     1741 gaaccgaaat tggattttga tcgagtggca aaaatccgca agctgaccgg aaaaccgatt
     1801 gtcatgcatg gcggatccgg tgtcagtcac gatgattatc gaaaagtgat tgcccgcggt
     1861 gtacgaaaaa tcaactatta cacgtatatg gcacgtgcgg gagccgataa tgtgaaaaag
     1921 catttggcgg aagccaaagg cacggttctg tttgaacata tcgaatcctg ggcgatggaa
     1981 gcgattcacg atgatgtgaa aaagacaatt tcggtgtttt gggagagtca gacacccgtt
     2041 gtttaaagca acaaaaatca aatccgcttt gaaagaagcg gattttttta ttt
//
LOCUS       DAOMIV010000382         2093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_60143_length_2093_cov_2.723749, whole genome shotgun sequence.
ACCESSION   DAOMIV010000382 DAOMIV010000000
VERSION     DAOMIV010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60143_length_2093_cov_2.723749"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..1404)
                     /locus_tag="PKV59_08230"
     CDS             complement(271..1404)
                     /locus_tag="PKV59_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HQP46774.1"
                     /translation="MKKQIDLQQFPAGNLPARILMGPGPSMVDPRVYQAMAAPLVGHL
                     DPEFVHLMDRTQELLRYTFRTENKLTIPVSGTGSAAMEASIANSVEPGTPVLICINGY
                     FGERLADMAKRYGGDVSTIHRPWGEVFTPSEIEQALREKPAKVVAIVHAETSTGAEQP
                     LDEIAKIVHNHGAILIVDAVTSLGGVPLSVDEIGIDICYSGTQKCISAPPGLGPITFN
                     ERALNLMQSRKAPVTSWYLDMSLVQAYWGPNRTYHHTAPITMNYALYEALRIIAEEGI
                     ENRWARHRANSEMFWEGLEDLNLKCFIPKEHRLTSLCTIQIPEGVDDAAVRKSLLEDY
                     NIEISGGLGSLKGKIWRVGLMGYTSRKENILLLLAALKKILYK"
BASE COUNT          633 a          429 c          415 g          616 t
ORIGIN      
        1 ctcctttctg cccttacgga ggacatatga tcgaacaagt gcgaatggca gcaacggagg
       61 ctttgaaaac agatgtttac gtggatttac gctttgaccc atgggatccc gaaatgatgg
      121 aagaaggagc ggatatggat tggggattcc tgtaatcttt tctctttcgg ggacgattct
      181 acaaaaattt gaataaaaga aggaggtgca tcgaaatcag actaagatgc acctccttta
      241 ttttttgtta aaaatttcaa atgagataaa ttacttgtaa agtattttct tcaatgcagc
      301 caaaagcagc aatatattct ctttccttga ggtatatccc attaagccaa cccgccagat
      361 cttacctttc aggctgccca atccaccgga aatttcgatg ttgtaatcct ctagaagtga
      421 ttttcgaacc gcggcatcat ccaccccttc aggaatctga atcgtgcaaa gagatgtgag
      481 acggtgttct ttcggaataa aacactttag gttcaaatcc tctaaacctt cccaaaacat
      541 ttccgaattt gcccgatgac gtgcccagcg attttcaatt ccctcttccg cgataatccg
      601 aagtgcctca tataacgcat aattcatagt aataggtgcg gtgtggtgat aggttcggtt
      661 aggaccccaa taagcttgaa caagtgacat atccaaatac cagctggtca ctggtgcttt
      721 tctggattgc attaaattta aagctctttc attgaaagtt atcggtccca atccgggagg
      781 tgcactgatg catttttgag ttccgctgta acaaatatcg attccgattt cgtccacact
      841 taacggtaca cctccgaggg aagtaactgc gtcaacgatc agaatcgctc cgtgattatg
      901 gacgattttg gcaatttcat ccagcggttg ttccgcgccg gttgaagttt ccgcatggac
      961 aatcgcaacg acttttgccg gcttctcccg tagagcctgt tcgatttcgg aaggggtaaa
     1021 aacttcaccc cacgggcgat gaatcgttga aacatctcca ccatatcttt tcgccatatc
     1081 ggcgagccgc tcaccgaaat aaccgttgat gcaaatcaaa accggcgttc ccggttcaac
     1141 cgagttggcg atggaagctt ccattgcggc actcccggtt ccagataccg gaatcgttaa
     1201 tttgttttcc gtacggaaag tatatcgaag caattcctga gttcgatcca tcaaatgaac
     1261 aaattcaggg tctaaatggc cgactaaagg ggctgccatt gcctgatata ctcgcggatc
     1321 gaccatactc ggtcccggac ccattaaaat acgtgcgggt agatttccag ccggaaattg
     1381 ttgcagatct atttgttttt tcattccata tcctccgatt tgtgtttaac atggtctgaa
     1441 ttatgttctc ttattgataa tactcgtaaa aaaagaaatg ggaagttata aattttcatt
     1501 tgtcagaaac ccataaaaga tctcctccat atattcttta aagaatataa agaatcgaac
     1561 aattattatt attatattga ctttaagaat taggtgtcaa gcagatttaa tgaacaataa
     1621 tttattgatt taagaattat ccctccgtat gatttgaatg gtttgtttct ttgatttcga
     1681 tacggacccg taggtgatat tataaataaa aactctttca atcaacattt ctaaaagatc
     1741 gaatttctat ctatattctt tcaagaaaga acggaaagag cccaactatt cccaaggata
     1801 tatgatagaa ttatacggat tagttttttt taccatacag ataatatgat aattttagta
     1861 aataaataat ataagagatc aataaatata gaaaagaaaa aaataaacaa cggagaaaaa
     1921 gcataatgac aaaaaaagtt ctttttaatg atttaacgtt gcgtgacggg catcaaagtt
     1981 gcgcggcgac tcgtatgaca accgaacagg ctatgcgtgt ccttcccatc ataaaaaatg
     2041 cgggatataa catgttcgaa ctttggggtg gggcgacctt agattcggca att
//
LOCUS       DAOMIV010000383         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_60508_length_2084_cov_3.700345, whole genome shotgun sequence.
ACCESSION   DAOMIV010000383 DAOMIV010000000
VERSION     DAOMIV010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60508_length_2084_cov_3.700345"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..405)
                     /locus_tag="PKV59_08235"
                     /pseudo
     CDS             complement(1..405)
                     /locus_tag="PKV59_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986176.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(545..1633)
                     /gene="arsB"
                     /locus_tag="PKV59_08240"
     CDS             complement(545..1633)
                     /gene="arsB"
                     /locus_tag="PKV59_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQP46775.1"
                     /translation="MNTKSENIGIGFFQKYLSIWVILCMVAGILIGKFLPGIPAFFGR
                     FEYANISLPTAILIWVMIYPMMLKVDFQSIKNVGKNPKGLYITWITNWFIKPFTMFAI
                     GSFFLYVVFKNIIPADLGKQYLAGMVLLGAAPCTAMVFVWSTLTKGDPAYTVIQVATN
                     DLIILVAFVPIVKFLLGISNVTVPWDTLILSVLLFVVIPLAGGFLTRVTVIKNKGIDY
                     FENIFLHKFDQVTIIGLLLTLILLFSFQGQTILRNPLHIVLIAIPLIIQTFLIFFIAY
                     FACRRAKLPYSIAAPAGMIGASNFFELAVAVAIALFGMDSPAALATTVGVLTEVPTML
                     ILVNIANNTRHWFPALEISSKKASEKSY"
     gene            complement(1653..1973)
                     /locus_tag="PKV59_08245"
     CDS             complement(1653..1973)
                     /locus_tag="PKV59_08245"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQP46776.1"
                     /translation="MEDVYREYAPIFKALSDETRLKIVDMLSCGEMCACDILTEFNIS
                     QPTLSYHMKILSDSGLVFAKRDGAWMKYTINQEKSNTLIDFLSRLMKEKDDCICKKYQ
                     QNSK"
BASE COUNT          663 a          464 c          370 g          587 t
ORIGIN      
        1 atcctttgat tgagaaaggt tgattacaca atttgaaata attacatcca ccaaaccgtc
       61 cgccaccgga agatgttcga tttctcccag acggaattcc acatttgtgc atcccatttt
      121 ctttgcgttt tctctggcca ataaaaccat ttcgggagtc atgtcaattc cgatgacata
      181 accgtcttta ccgaccatct tttcggcaag aaaacaatca aatccgccgc cgctaccgag
      241 atccaataca atttcccctt ctcggatagc ggctatggtc agaggattcc cgcatccgag
      301 acccatattg gcttccgccg gagccgataa taaatcttct ttggaataac catttcgcaa
      361 agccgcttcg gttatgccaa cagtaacaga agaaccgcaa catccgctgc ctgcgcagca
      421 accggtagga aggtctcggt tcgcgatcgc cgaatacttt tcaatgatta ttttttgtgt
      481 tttattttta ttctgttcca tctgtcaata atcctttacg aatgttcaaa agtccgcaat
      541 gtacctaata tgatttttcg gaagctttct ttgaacttat ttctaaagcc gggaaccaat
      601 gtcgtgtatt gtttgcaatg ttaaccaaga tcaacatcgt aggcacttcg gtcaatacac
      661 cgactgtcgt agccaaggca gcgggagaat ccattccaaa caaagcgatg gcaaccgcaa
      721 ccgccagttc gaagaagttt gaagcaccga tcattccggc cggtgcggca atcgaatagg
      781 gtaattttgc tcgacgacaa gcaaaataag caataaagaa aatcaaaaaa gtttgaataa
      841 tcaagggaat tgcgatcagc acaatgtgta gcggattcct caaaatcgtt tgtccttgga
      901 aagaaaataa taggattaaa gttaagagca atccgattat cgttacctga tcaaatttat
      961 ggagaaaaat attttcaaag taatcgatgc ccttgttttt gataaccgtc actctggtca
     1021 ggaaaccacc cgcaagcgga atcaccacaa acaataaaac cgataggatc aaagtgtccc
     1081 atggaacggt tacattgcta attcccagca agaattttac gataggaacg aatgcaacca
     1141 aaatgatcaa atcatttgtc gccacttgta taacggtata agccggatct cccttggtta
     1201 aggtactcca aacaaaaacc atagccgtac aaggcgcggc accaagcaat accatgcccg
     1261 ctaaatactg tttcccgaga tccgcaggaa taatgttttt aaacacaacg taaaggaaaa
     1321 aagatccgat cgcaaacatc gtaaaaggtt taatgaacca gttggtaatc caggtaatat
     1381 ataaaccttt tggattctta ccgacatttt taatgctttg aaaatctact ttcaacatca
     1441 tcggataaat catcacccat atcagaatcg ctgtcggtag cgaaatgttt gcatattcga
     1501 acctgccgaa gaatgccggt atccccggaa gaaattttcc gatcaaaatg ccggcaacca
     1561 tacataaaat aacccaaatc gataaatatt tttggaaaaa tccgattccg atattttccg
     1621 actttgtatt cataagtaaa aatccttctt ttttatttgg aattctgttg atattttttg
     1681 caaatacaat cgtctttttc tttcataaga cggctgagga agtcaataag cgtattggac
     1741 ttctcttgat taatcgtata tttcatccat gctccgtctc tcttcgcaaa taccaaaccg
     1801 ctgtcggata aaatcttcat atgataactg agtgtcggct gggaaatatt gaattccgtt
     1861 aaaatatcac aagcacacat ctccccacag gacaacatat cgacaatttt taagcgagtt
     1921 tcatccgata aagccttaaa aatcggtgca tactccctat agacatcctc catgaattac
     1981 cttcctcatc atatagaatc atttctatga tatagatatt tatctatatg atagaagtga
     2041 ttctatcata tcggaaaaat gttgtaaatc ggaagatctt agaa
//
LOCUS       DAOMIV010000384         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_60808_length_2077_cov_4.846686, whole genome shotgun sequence.
ACCESSION   DAOMIV010000384 DAOMIV010000000
VERSION     DAOMIV010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60808_length_2077_cov_4.846686"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PKV59_08250"
     CDS             <1..842
                     /locus_tag="PKV59_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HQP46777.1"
                     /translation="NQFDELGNYLWHYHVTGNAMREVSEKLMKKNDRFAGICSNTGSG
                     GTIACGDYLKTLHPNMKIVASEALQCPTLLMNGFGAHRIEGIGDKHVPWIHNVRNTDF
                     VTSIDDNACVHLLRLFNEPEGKKYLVEKGVPKEIVEQLNLLGISSVANLLSAIKFAKY
                     NELTGEDMVMTVFTDSVDMYTTRLAEYNEEFGPFTRDDASYAFGRYLMGENTSNMLEL
                     SYYDRKRIHNLKYYTWIEQQGKTSEELNAQWYDRNYWTEIAAQKDEIDALIKSFNEQV
                     GLL"
     gene            complement(932..1495)
                     /gene="pdxT"
                     /locus_tag="PKV59_08255"
     CDS             complement(932..1495)
                     /gene="pdxT"
                     /locus_tag="PKV59_08255"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HQP46778.1"
                     /translation="MTKIGVLALQGAFIEHCQILRRLAVDCVELRKPADISQDLDGLI
                     LPGGESTVMGKLLNDLGMLIPIRKMISEGLPVFGTCAGMILLAQRLSEDSIPRPGTMD
                     ITVRRNAYGRQIGSFNTVGTFAGKRVPMTFIRAPYIEEAGTDVQILSVEDGHIVAAEQ
                     GNQLVTAFHPELTDDPTVHQHFLDMIS"
     gene            complement(1492..>2077)
                     /locus_tag="PKV59_08260"
     CDS             complement(1492..>2077)
                     /locus_tag="PKV59_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="with PdxT forms pyridoxal 5'-phosphate from
                     glutamine, either ribose 5-phosphate or ribulose
                     5-phosphate, and either glyceraldehyde 3-phosphate or
                     dihydroxyacetone phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HQP46779.1"
                     /translation="DYIDESEVLSPADDIYHIDKTKFKVPFVCGARDLGEALRRINEG
                     ASMIRTKGEPGTGDIIQAVRHMRKMNAEIRRIQSISEDELFEAAKVLEVPYDLLKNVH
                     ENGRLPVVNFAAGGIATPADAALMMQLGAEGVFVGSGIFKSGNPAKRANAIVRAVTNY
                     QDPILLAELSEDLGEAMVGINENEIAILMEERGK"
BASE COUNT          580 a          516 c          439 g          542 t
ORIGIN      
        1 tcaatcaatt cgatgagttg ggtaattatc tttggcatta tcatgtgacc ggaaatgcca
       61 tgcgtgaagt ttcagaaaaa ttaatgaaga aaaacgatcg atttgccggt atttgttcca
      121 atacgggttc cggcggcaca atcgcctgcg gagattacct caagacactc cacccgaata
      181 tgaaaattgt cgccagcgaa gctttgcaat gcccgacttt attaatgaac ggtttcggcg
      241 ctcatcggat tgaaggaatc ggcgataagc atgttccttg gatccataat gtccgcaata
      301 cagacttcgt aacttcaatc gatgataatg cttgtgttca tttgctgagg ctcttcaatg
      361 aaccggaagg aaaaaagtac ttggttgaaa aaggcgttcc gaaagaaatc gtcgaacaac
      421 tcaacctgtt gggaatctcc agcgtagcca atttgctgtc ggcgattaag tttgccaaat
      481 acaatgaact gaccggcgaa gatatggtaa tgaccgtttt caccgattcg gtcgacatgt
      541 atacaacccg gttggccgaa tacaacgaag aattcggacc atttacccgt gatgatgcct
      601 cttacgcatt cggtcgctat ttgatgggtg aaaatacatc gaatatgctg gaattgtcct
      661 attatgatcg gaaacggatt cacaatttga aatattacac atggattgaa cagcagggca
      721 agacgtccga agaattgaat gcccagtggt atgaccgcaa ttattggacg gaaatcgccg
      781 cccaaaaaga tgaaatcgac gctttgatta aatccttcaa tgaacaggtt ggcttactgt
      841 aaatcagagg aatgcggcat ttatattcca tagccgtaca ttcagaaagt cccgcatcga
      901 tcgatgcggg acttttgcat ctcaataaaa gctatgaaat catatctaaa aaatgctgat
      961 gaaccgtcgg atcatccgtt aattccggat gaaaagccgt caccaattgg tttccttgct
     1021 ccgctgccac gatgtgtcca tcctctacgg aaaggatttg aacgtctgtg cctgcttctt
     1081 cgatataggg cgcacgaata aaagtcatgg gaacccgctt tccggcaaat gtccccaccg
     1141 tattaaaact cccgatttgc ctcccatacg cattccggcg aacggttata tccatcgtcc
     1201 ccggacgggg gatcgaatct tccgaaagcc tttgagccag taaaatcatc ccggcacaag
     1261 taccaaatac cggtaatccc tccgaaatca ttttgcggat cgggatcagc attcccaagt
     1321 cattcagcag cttacccatc acagtgcttt caccgccggg caaaattaaa ccgtctaaat
     1381 cttgagagat atcggccggt ttgcgcaact ctacgcaatc aacagccaaa cgacgaagaa
     1441 tttgacaatg ttcgataaag gctccctgca aggcaagcac accgattttg gtcattttcc
     1501 gcgttcctcc atcagaatag cgatttcatt ttcgttgatt cccaccattg cttcgcccaa
     1561 atcttcggac aattccgcca aaagaatcgg atcttgataa ttggtgaccg ctctgacaat
     1621 ggcattagct cgcttggcag gatttccgga tttgaaaatt ccggaaccga caaatacacc
     1681 ttccgctccc agctgcatca ttaaagcggc atccgccggg gtggcaatcc cgccagcggc
     1741 aaaattcact acgggcagtc tgccgttctc atgaacattt tttaataaat cgtatggtac
     1801 ttccagaacc ttggcagcct caaacagctc atcttccgaa atcgattgga tccggcgaat
     1861 ttccgcattc atcttccgca tatgacggac agcctgaata atatcccccg ttcccggctc
     1921 accttttgtc cgaatcatcg atgctccttc attgattcgg cgtaaagctt cgcctaaatc
     1981 ccttgcgccg cagacaaagg gaactttgaa ttttgtttta tcaatatgat aaatgtcatc
     2041 agccggcgac aatacttcac tctcatcaat atagtcg
//
LOCUS       DAOMIV010000385         2077 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_60823_length_2077_cov_3.079624, whole genome shotgun sequence.
ACCESSION   DAOMIV010000385 DAOMIV010000000
VERSION     DAOMIV010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60823_length_2077_cov_3.079624"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="PKV59_08265"
     CDS             <1..1112
                     /locus_tag="PKV59_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQP46780.1"
                     /translation="VSICENSAKKSRGKFMDYRECLERLAASGHIEQKNGLQIHVKPV
                     PETDCPGVVDPRAANWFRNLPKGYQVQDYRRITSEEVQILRDAMGCRNADLSRGVKTN
                     RRTINTENGDVPILIYTPDISGTLPLICYIHGGGFFGGSTEVVENACRLLAERAKSVV
                     ISIDYGLAPEHRFPEGLLQCWGVIRWVHSHAELLNIDPQRIAVSGDSAGGNLSMACCL
                     LDNEGIIRAQLLLYPAIVLRSDIDFGWTWDVYEMNEGKDAALMMVHDIKDTVGRIIWM
                     YIRDITDTYSQLLSPWQAPAFAKFPPTFLAVGEYDYLRQEAELFCQRLVKTGIPVAFF
                     RYQGMGHAFFEHTGEFPQAEDCTNKMAEFLSSIWS"
     gene            1364..1434
                     /locus_tag="PKV59_08270"
     tRNA            1364..1434
                     /locus_tag="PKV59_08270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1396..1398,aa:Gln,seq:ctg)
     gene            complement(1647..1889)
                     /locus_tag="PKV59_08275"
     CDS             complement(1647..1889)
                     /locus_tag="PKV59_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46781.1"
                     /translation="MARIRCKYLDCAFLDENYCTAALIELDSDKGCLTYSPVAETPVE
                     DDWEETEEVEDWEDLDEDEKEDDLWIDDEDDEEEEF"
BASE COUNT          556 a          408 c          475 g          638 t
ORIGIN      
        1 cagtttcgat atgcgaaaat tcagcaaaga agagtagggg gaaatttatg gattatcggg
       61 aatgcttgga acgacttgcc gcatccggac atattgagca gaaaaatggt ctacaaattc
      121 atgttaaacc ggtaccggag acagattgtc ccggtgtggt tgatccgcgg gcggcaaact
      181 ggtttcgcaa ccttccgaaa ggatatcaag ttcaagatta tcggcgaatt acttcggagg
      241 aagttcagat tctgcgggat gcaatgggat gtcgaaatgc cgatctgagc cgaggtgtga
      301 aaacgaatcg cagaacgatt aacaccgaga acggtgatgt tcctattctc atctatacgc
      361 cggatatctc gggaactctt ccgcttatct gttatataca tggcggaggt ttttttggag
      421 gttcgacgga agtggtcgag aatgcctgtc gtctcttggc ggagcgggca aagtctgttg
      481 taatctcaat tgattatggg ttagctccgg aacatcgctt tccggaagga ttattgcagt
      541 gttggggcgt gattcgctgg gttcattctc atgcggaact tttaaatatt gatcctcaaa
      601 gaatcgcggt ttccggtgat tcggcgggag gaaatctttc gatggcttgt tgcttgttgg
      661 ataatgaagg catcattcga gctcaacttt tactttatcc ggccattgtt cttcgatcgg
      721 atatcgattt tggttggacg tgggatgtat atgagatgaa tgaagggaaa gatgccgctt
      781 tgatgatggt gcacgatatc aaagataccg tcggacgcat catttggatg tatatcagag
      841 atataaccga tacctacagc caattgcttt ctccttggca ggcaccggct tttgcgaaat
      901 ttccgccgac ttttttagct gtcggcgaat atgattatct ccgtcaggaa gcggaattgt
      961 tttgccagag attggtaaaa accggtatcc cggtggcttt ttttcgatat cagggaatgg
     1021 ggcatgcttt ttttgaacat accggagaat ttccgcaagc cgaagactgt acgaataaaa
     1081 tggccgagtt tttatcctca atttggtcat aaagaaagga atgatttcta tgggctcgag
     1141 gagattttcc ccctttttga gcccatggta ttctttatcg tgacaatctc atccggttca
     1201 agtgcgagac cggcatcatc ctctctcttt tttctctttg gcagggcttg gcggaatcgc
     1261 cgggagttta tcggaatgcc atgattgccg aataacaata ctgtcaagac gattcacttt
     1321 acggattttt ttaatggtga tagaatacaa aacgccgaag aattggggac tcgtctaatg
     1381 gtaggacagc agactctgaa tctgtatgta gaggttcgaa tcctttgtcc ccagccttta
     1441 taaaatccga atcatttcat tcggattttt ttattatagg ccaaataaaa ctatttattt
     1501 ttatatagta aatgtcctta aaaagggata tgcgattttt caccgcatat ccctttttaa
     1561 actcatcaat cggatcaata ttcagttgtg gaaattggca aagactgccg aattcttttg
     1621 tataaagaga atcggagtcc tactgattaa aattcctctt cctcatcatc ctcatcatct
     1681 atccaaagat cgtcttcttt ttcatcctca tccaaatctt cccaatcttc aacctcttcc
     1741 gtttcttccc aatcatcttc aacgggagtt tcagcgacag gggaataagt gaggcaacct
     1801 ttatccgagt ccagctctat taaagccgcc gtgcagtagt tttcatccaa aaaagcgcaa
     1861 tcaagatatt tgcatcgaat tcttgccatt gttaaacctc ttctctatga atttgccgaa
     1921 agtattcaat gtcgactagc aaattttgcg ctattctacc ctatacggtt ttctatgtaa
     1981 aggataaaag agggggaaag tataagaatt ttattaatat tttttgttgg gaagtgatta
     2041 agaatattca ggagaaaaag atcgatgaag caacttg
//
LOCUS       DAOMIV010000386         2075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_60912_length_2075_cov_4.043069, whole genome shotgun sequence.
ACCESSION   DAOMIV010000386 DAOMIV010000000
VERSION     DAOMIV010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60912_length_2075_cov_4.043069"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..445
                     /locus_tag="PKV59_08280"
     CDS             107..445
                     /locus_tag="PKV59_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQP46782.1"
                     /translation="MEKKPRVSGSTMIAPEVIETIIKMTAMETDGVSKIPAQLTHQPV
                     RIRINEEKKIDADVYVRIEYPYNVIHVGKKLQENINRVISETIGMEPGSINIHIEDID
                     YLNNSEKERS"
     gene            457..1008
                     /gene="nusB"
                     /locus_tag="PKV59_08285"
     CDS             457..1008
                     /gene="nusB"
                     /locus_tag="PKV59_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQP46783.1"
                     /translation="MSETNRELKPEDRDNHKTLDNKDNPDSSQDEIIESVVIDNIELD
                     DRTRARGVALQVLYEMDMSDHPINLILQERIQSVPLDEQQTKLVLKIVLGLQPYIHYI
                     DDFIEKYAPEWPIDQIAVIDRNIIRIAVWEFAIGEITPIKVAINESVELAKLFGSESS
                     PRFVNGVLGSLAEHIKDIQKIKS"
     gene            1198..>2075
                     /locus_tag="PKV59_08290"
     CDS             1198..>2075
                     /locus_tag="PKV59_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HQP46784.1"
                     /translation="MENVGIPGKYGLENYGIKNAGKVYWNAVPAILVEESVKRNEGVL
                     SPDGALMVNTGKYTGRSPNDKFIVDNGTEEEKHIDWGKVNVKMSPEHFNKIYDKVLGY
                     VQNRDLFVADLVAGADPVYRLPMRIITEFAWSNLFITDMLIRPSLEERIGQIPGFTII
                     CVPGLESIPEEDGTHSGAFIIVNFAKKLVLIGGSHYGGEIKKSVFTVMNFILPLKGVM
                     TMHCSANVDKKTGDSALFFGLSGTGKTTLSSDPNRLLIGDDEHGWSDTGIFNFEGGCY
                     AKMIKLSKEHEPLIWNA"
BASE COUNT          710 a          339 c          434 g          592 t
ORIGIN      
        1 aatgggttca agaaaaggga aaacatgatt taaaattcat gttcatattt catttataat
       61 aagctcatgt ttaaaacaaa aaagagatga ggttggagta aaagaaatgg aaaaaaagcc
      121 acgtgtttca ggaagcacga tgattgctcc cgaagtaatt gagactatca taaaaatgac
      181 agcgatggaa acggacggtg tcagtaagat tcccgcacaa ttaactcatc aaccggtaag
      241 gatacgtatc aatgaagaaa aaaaaattga tgcggatgtt tatgtgagaa tcgaatatcc
      301 ttataatgtc attcatgtcg gcaaaaaatt gcaggaaaat ataaaccgtg tgatttcaga
      361 aacgattgga atggaaccgg gctcaataaa tatccatatt gaagatatcg attatcttaa
      421 caactcggaa aaagaaagat cctgactgaa aatattatgt cggaaacaaa cagagaatta
      481 aaaccagaag atcgagataa tcacaagacc cttgataaca aagataatcc agactcttcg
      541 caggatgaaa ttatcgaaag tgttgtaatc gataacattg agctggatga ccgcaccaga
      601 gcccgtggag ttgctttgca agttttatat gaaatggata tgtccgatca tccgataaat
      661 ttaatccttc aggaaagaat tcaatctgtt ccgttagatg aacaacaaac aaagttggtt
      721 ttaaaaatcg ttctcggtct ccaaccatat attcactata tcgacgattt tatcgaaaag
      781 tatgccccgg aatggccgat cgatcaaatt gccgtaattg atcggaatat tattcgaatc
      841 gctgtatggg aatttgctat cggagaaatt acaccgatta aagttgcgat caatgaatcc
      901 gtcgaattag ctaagttatt cggttccgaa agctctccga gatttgtaaa cggtgtctta
      961 ggaagcctgg cggaacatat caaagatatt caaaaaataa aatcgtaaat attttcgtcc
     1021 actttttttg gaaatcataa atcagatgta tttaatttat tcataactgt catatttatt
     1081 tcgggaaatt ccgagtataa tctgatacat tggaagtttt tttatgaaag aaataaatac
     1141 ttcccgtaat atttaacaca aatttttgta aaaaaattca aaatggagga aagctttatg
     1201 gaaaatgtag gtatacccgg aaaatatggg ctggaaaatt acggtattaa gaatgctggt
     1261 aaagtttatt ggaatgcggt acctgcgatt ctggttgaag aatcagttaa aagaaatgaa
     1321 ggtgtgcttt ctccggatgg cgctttgatg gttaacaccg gtaaatatac cggcagatca
     1381 cctaacgata aatttattgt tgacaatggt acagaggaag agaaacacat tgattggggc
     1441 aaagtaaatg ttaaaatgtc ccccgagcat ttcaataaaa tttacgacaa ggtgttaggg
     1501 tatgttcaaa accgagattt atttgtagct gatttggttg cgggagcaga tcctgtctat
     1561 cggttgccca tgagaattat cactgaattt gcatggtcaa acttatttat aactgatatg
     1621 ctgattcgcc cgtcattaga agaacggatt ggacaaattc ccggatttac aattatttgt
     1681 gtccccggct tagaatcgat tcccgaagaa gacggcacac attcgggggc atttattatt
     1741 gtgaattttg caaagaaact ggtattgatc ggcggctctc attacggtgg agaaataaag
     1801 aaatccgtat tcactgttat gaatttcatt ctcccattaa agggagtaat gaccatgcac
     1861 tgctccgcta atgtcgataa gaaaaccggt gattcggctt tgttctttgg attatccggt
     1921 accggaaaaa ccactttgtc ttccgatccc aatcgtttat taatcggaga cgatgaacac
     1981 ggctggagtg acacgggtat tttcaacttt gaaggcggat gctatgcaaa gatgatcaaa
     2041 ttgagtaagg aacatgaacc gctcatttgg aatgc
//
LOCUS       DAOMIV010000387         2069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61210_length_2069_cov_3.996028, whole genome shotgun sequence.
ACCESSION   DAOMIV010000387 DAOMIV010000000
VERSION     DAOMIV010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61210_length_2069_cov_3.996028"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="PKV59_08295"
     CDS             complement(<1..802)
                     /locus_tag="PKV59_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HQP46785.1"
                     /translation="MSILLTLLIVFSAFSPVLAQSQTAEQTISETSTSFSKLYEEVNP
                     SVVYISVAIQGGSSNTQQFQGLPDDLEELLPFLKQFYGPQGQNNQDQESEPNQNQNQN
                     QNQNQNQNITYGSGTGFVWDTEGHVVTNNHVIEDATDITVTYYDGIERKATVIGADPD
                     SDLAVLSVEDYRKDIAPVKMGDSDTVKTGEYVAAIGNPFGNVGTITMGIVSALGRNFP
                     IDDGSTGSGGHYTIPDMIQTDAAINPGNSGGVLINLNGEVIGVVNSFVS"
     gene            complement(1003..1656)
                     /locus_tag="PKV59_08300"
     CDS             complement(1003..1656)
                     /locus_tag="PKV59_08300"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HQP46786.1"
                     /translation="MADDHYKIFSCKLIPNIPPERILGVRLPDLRKLAQRIAGEDPES
                     FFADAGDETFEEKMLQGMVICYLDGSFNEKLALVEKFIYKIDNWSVCDSFCAGLKLPL
                     KYPGEMWDFITPYLQDQREYFIRFGSVMLLSYYVDETHLHAALSLLDQVRSRDYYART
                     GVAWAISEYFSHFPEETMRYLHESSLDDQTFNKVLQKIIESRFTDAKMREEIHALQR"
     gene            complement(1700..>2069)
                     /locus_tag="PKV59_08305"
     CDS             complement(1700..>2069)
                     /locus_tag="PKV59_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQP46787.1"
                     /translation="LPTIKMDENRMYQAVGNLLSNAIKYSPNGKTVTFRAVQTDDEIQ
                     IQVTDQGCGIDPEDLPHIFESFYRGSENKRIIQGMGLGLTISQDVVQAHRGTITVESE
                     KGAGSTFTIHLPLKNISPEK"
BASE COUNT          538 a          455 c          463 g          613 t
ORIGIN      
        1 ctgaaacaaa tgagttaacc actccgatga cttcgccgtt gaggttaatc agaacgccgc
       61 ctgagttccc cgggttgatt gcggcatccg tttgaatcat gtccggaatg gtataatgtc
      121 cgccactgcc cgttgacccg tcgtcaattg ggaaattacg gccgagagcg gatacgattc
      181 ccatggtgat ggttccgaca tttccgaacg gattgccgat tgctgcaaca tattcgcccg
      241 tctttacggt atctgaatca cccattttca cgggagcgat atcttttctg taatcttcca
      301 ccgacagaac cgctaaatca ctgtccggat cggcgccgat taccgtcgct ttccgttcga
      361 ttccgtcata ataagtgacg gtgatatcag ttgcgtcttc gatgacatga ttattcgtga
      421 caacgtgtcc ttccgtatcc cagacgaaac cggttcctga tccataagta atattctgat
      481 tctgattttg attctggttc tgattctgat tctgattcgg ttccgattcc tgatcctgat
      541 tgttttgtcc ctgcggacca tagaattgct tcagaaacgg aagcaattct tccagatcgt
      601 ccggcaatcc ttggaattgt tgggtgttgg aagaccctcc ctgaatggct acggaaatat
      661 agacaacgga gggattgact tcttcataca atttgctgaa cgaagtgcta gtttcgctga
      721 tcgtttgctc agcggtttga ctttgagcaa gaaccggaga aaaagcactg aaaactatca
      781 gcaatgtaag aagaatcgac aaaatagtgt ttctagttgt gcttttcata ttttttttac
      841 cctttcttat ttcttttttg ttaaataagg cattttcccc taatttgatc attattaagc
      901 ctactacgaa aatattaata gaacattaat ttttattgaa gaagaacatt atttttatct
      961 taaggtggga gttaagcaga aatttggctt attaaagctg tattatcgtt gcagagcatg
     1021 aatttcctca cgcattttgg catccgtaaa gcgagattcg atgattttct gaaggacctt
     1081 attaaaggtc tggtcatcca aggagctctc atgaaggtag cgcatggtct cttcgggaaa
     1141 atgcgaaaaa tattcggaaa tcgcccaggc tactcccgtt cgcgcgtagt agtctcggct
     1201 tctgacttga tcaagcagag ataaagcagc gtgcaagtga gtctcatcga cataatagga
     1261 aagcagcatg accgatccga aacggatgaa atattctctt tgatcctgta aataaggagt
     1321 aataaaatcc cacatttctc cgggatattt caacggaagt ttcaagccgg cacaaaaact
     1381 gtcgcaaacg gaccaattgt ctattttata gataaatttc tcaaccagag ccaatttctc
     1441 gttgaaagag ccgtcgagat agcatatcac cattccctgc agcatttttt cttcaaaagt
     1501 ttcatcgccg gcatcggcaa aaaaactttc gggatcttcg ccggcaatcc gctgcgcaag
     1561 ctttcgtaaa tcggggagac ggactcctaa aattcgttcc ggcggaatat tcgggataag
     1621 cttgcaactg aagattttat aatgatcgtc agccatggaa acgagctgtt gtcggattgg
     1681 aagacctgtc cggttcatac tatttttcgg gcgaaatgtt cttgagcgga aggtgaatgg
     1741 tgaaagtgct gccggcgcct ttctcgcttt cgacggtaat cgtccctcga tgagcttgga
     1801 cgacgtcctg agagatagtc aaacccagcc ccattccttg gatgatgcgt ttattttccg
     1861 aacctcgata gaaggattca aaaatatgcg ggagatcttc tggatcaatg ccgcagcctt
     1921 gatcggtcac ttgaatttga atctcgtcat cggtctgaac tgctcgaaag gtgacggtct
     1981 tcccgttggg tgaatattta atggcattgc tcaataaatt cccgacggct tgatacatgc
     2041 ggttttcatc cattttgatt gtcggcaga
//
LOCUS       DAOMIV010000388         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61357_length_2066_cov_3.715067, whole genome shotgun sequence.
ACCESSION   DAOMIV010000388 DAOMIV010000000
VERSION     DAOMIV010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61357_length_2066_cov_3.715067"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PKV59_08310"
     CDS             complement(<1..1078)
                     /locus_tag="PKV59_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQP46788.1"
                     /translation="MKQRGQTNIVYLLLLVGIISLLLYSFTGNRSANNVISINQLANQ
                     IKQGNIAKIESNSTTVNILYKDRSVAKAVIEPNSSLLEQLTLLGVTPEELSSDNIEIS
                     IVEESSWVGILSILSYFLPVIFLALAMFFIFRQTQSGNNSALSFGKSRAKMFEGDHPT
                     VTFADVAGVDESKEELQEIVEFLKEPQKFTRLGARIPKGVLLVGSPGTGKTLLAKAVS
                     GEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKKNSPCIIFIDEIDAVGRQRGAGL
                     GGSHDEREQTLNQMLVEMDGFETDTNVIIIAATNRPDILDPALLRPGRFDRQVVLDRP
                     DVVGREAILKVHSKGKPLDKDVDLN"
     gene            1434..>2066
                     /locus_tag="PKV59_08315"
     CDS             1434..>2066
                     /locus_tag="PKV59_08315"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HQP46789.1"
                     /translation="MNIDYKAIKAKAESYADDMTKFMCDLIAIPSESCNEKGVIYRIK
                     EEMEKVGFDKIEIDPMRNILGYIGHGSHLIAMDAHIDNVGVGEIKNWTVTGPYEPKVI
                     GDAIYGRGGSDQEGGMVSMVYAGKIIKDLGLEDDYTLLVVGSVQEEDCDGLCWQYIIN
                     EDKIRPEFVLITEPTSLRIYRGHRGRMEIKVHTHGISCHGSAPERGDNAIY"
BASE COUNT          609 a          430 c          460 g          567 t
ORIGIN      
        1 cattcagatc gacatcttta tccaacggtt tccctttgga atgaactttc aaaattgctt
       61 cccgtccgac gacatccggg cgatccaaaa ccacctgccg gtcgaaacga ccgggtcgaa
      121 gcaatgccgg atccaaaata tcaggccggt tggtcgccgc aatgataatc acgttggtgt
      181 ccgtctcaaa accgtccatt tcgaccagca tctggttcaa tgtttgttcc cgttcgtcat
      241 ggcttccgcc taatccggct ccgcgctgcc ggcctaccgc atcgatttca tcgatgaaga
      301 tgatgcaggg gctatttttc tttgcctggt caaacagatc gcggacacga ctggcgccga
      361 cgccgacgaa catttcaaca aattcggagc cgctgatgga aaagaagggt acaccggctt
      421 cgccggaaac tgcttttgcc agcaacgttt ttccggtccc gggtgaaccg accagtaaaa
      481 cgcccttcgg aattcgcgct cccagccgag taaatttttg cggttccttc agaaattcga
      541 caatttcctg aagctcctcc ttggattcat cgacaccggc gacatccgca aaagttaccg
      601 tcggatgatc accttcgaac atttttgccc gcgattttcc gaaggaaaga gcggaattgt
      661 tgccgctttg cgtttgccgg aaaataaaga acatagccaa tgccaaaaat atgaccggca
      721 agaaataact gagaatggat aaaattccaa cccagcttga ttcttcgacg atggaaattt
      781 caatgttgtc cgatgaaagt tcttccggag ttacgcccaa taaggtcagc tgttccaaca
      841 aagaagaatt gggctcgatt acggcctttg ccaccgagcg atctttataa agaatgttaa
      901 cggttgtcga attggattcg attttggcaa tattgccttg tttaatctga ttagccaatt
      961 gattgatgga aataacatta ttagcagagc gattccctgt aaatgaatat aacagtagag
     1021 atataattcc taccagtaat agcaagtaaa cgatatttgt ttgtcctcgt tgtttcacga
     1081 ataaatcctc ccttaatcac tatcggaatt atacaatgct aatgcaatgt tcccattaat
     1141 tgattatgtt aataaatttc tttcaaattt aattataatg aaatgcttct ttttttctga
     1201 aaatggtgaa agcggaaccc cggaatttca gaaaaaaagg ctgaaaacga tccggcgtaa
     1261 tttgtcatac aaataaaagt acaagaaatt gacaaaaaat aaccgttaat tttcaaccat
     1321 agtgtaaaat atcctaaagt ttgtgtttaa ttgtaagaca attaaattcc gtctgtattt
     1381 tttatggtta attttggttt cggccactta ttgaatatat tttggaggag aaaatgaata
     1441 tagattacaa agcgatcaag gctaaagctg agagctacgc tgatgacatg acgaaattca
     1501 tgtgtgactt gattgccatt ccgagtgaat cctgcaatga aaaaggtgtg atttatcgga
     1561 ttaaagagga aatggaaaaa gtcggcttcg ataaaatcga aatcgacccg atgagaaaca
     1621 ttctgggcta cattggtcac ggcagccatt taattgcgat ggatgcccat attgataatg
     1681 tcggagtcgg cgaaataaaa aattggacgg tgaccggtcc gtatgaaccg aaagtgatcg
     1741 gcgatgcgat ttatggacgc ggcggatcgg atcaagaggg tgggatggtt tccatggttt
     1801 atgcgggaaa aatcatcaaa gacctcggtt tggaagatga ttacacgttg ctcgtcgtcg
     1861 ggtctgtgca ggaagaagat tgcgacggtt tatgttggca atatattatc aatgaagata
     1921 aaatcagacc cgaatttgtt ctgattactg aaccgacatc actacgaatt tatcggggac
     1981 atcgcggtcg aatggaaata aaagttcata cccatggtat tagctgtcac ggttccgcac
     2041 ccgaacgcgg tgataatgcg atctat
//
LOCUS       DAOMIV010000389         2066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61372_length_2066_cov_2.712084, whole genome shotgun sequence.
ACCESSION   DAOMIV010000389 DAOMIV010000000
VERSION     DAOMIV010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61372_length_2066_cov_2.712084"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PKV59_08320"
     CDS             complement(<1..1172)
                     /locus_tag="PKV59_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46790.1"
                     /translation="MPPILELKGITKQFPGVLANDHINLTLEKGEILALLGENGAGKT
                     TLMNILYGLYKPDEGEIFIDGEKIEISGPIDAIRAGIGMVHQHFMLIPVFTVTENVML
                     GEESVNFGGVLNPEDAAKKIKAIGELYGLKVDPNAKVGDLPVGVQQRVEIIKLLYRNA
                     RILIFDEPTAVLTPQEADELFEIMRSLTKQGKSIIFITHKLREVLEFSDRIMVIQRGK
                     VVGETHPHEASQEKLAEMMVGRAVDLIPDKKKIQAGETVLKVDNLVVADGTRKITVHG
                     VSFSVHAGEILGVAGVQGNGQTELEEALTGMREPVSGTIRILGKEVPKVKPREFVENK
                     GAHIPEDRQRDGLILNFTVEDNLMLCTYYRPPFSQNHILNREAIRDHARKLITNFD"
     gene            complement(1353..>2066)
                     /locus_tag="PKV59_08325"
     CDS             complement(1353..>2066)
                     /locus_tag="PKV59_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46791.1"
                     /translation="NNPDFKFSIIDASVGKPNVVDQVFQTDEAAFLAGYVAAGMTKTG
                     IIGTFGGMCIPTVTIFMDGFARGADYYNQIHGTKVEVLGWNTETLEGTCVDSFDDLDK
                     GKQVSITMMDQGADIIMPVAGPVGGGTIAAMEDRKMGMVIGVDSDWYLTYPDNGDIIL
                     TSVMKKMDATTMQVIESVIDGTFKGGKVVGTLENKGVDLAPFHDFEDKVPADLVKELD
                     DIRAKIISGEIELNPVYAQ"
BASE COUNT          539 a          520 c          397 g          610 t
ORIGIN      
        1 atatcaaaat tggtgatcag tttacgggcg tgatctcgaa tcgcttcccg attgaggata
       61 tgattttgag agaacggcgg ccggtagtaa gtacaaagca tcaggttatc ttcgacagta
      121 aagttcaaaa tcaatccgtc tcgttggcga tcttcgggaa tatgcgctcc tttattttcg
      181 acgaattccc gcggttttac cttcggtact tcttttccta aaatacggat cgttccgctg
      241 acaggttcac gcattccggt caatgcttct tccaactcgg tctggccgtt tccctgaaca
      301 ccggcgacac ctaatatttc tccggcgtgt acagaaaaag aaacgccgtg aaccgtgatt
      361 tttctcgtgc cgtccgctac aaccaagtta tcgactttca gaaccgtttc acccgcctgt
      421 attttcttct tatccggaat caaatcaacc gcgcggccga ccatcatctc ggctaatttc
      481 tcctggctgg cttcatgagg atgtgtctcg cccaccactt ttccgcgttg aataaccata
      541 attcgatccg aaaattcgag aacctcacgc agcttatgag tgataaaaat tattgacttg
      601 ccttgtttcg tcagagaccg cataatttca aataactcat cagcttcttg gggtgtaaga
      661 acagcggtgg gttcgtcaaa aatcaatata cgggcatttc gatatagtaa tttgataatc
      721 tcaacccgtt gttgaacccc taccggcaaa tcaccgactt tggcattcgg atcgactttc
      781 agtccgtaca gttcaccgat cgcttttatt tttttggctg catcttcagg attcagaacc
      841 cctccgaaat tgaccgattc ttctcccaac atcacatttt ccgtgaccgt aaaaacagga
      901 atcaacataa aatgttggtg gaccattccg attcccgccc gaatcgcatc gatcggtccg
      961 cttatttcta ttttttcccc atcgataaaa atttcccctt catccggttt gtataaaccg
     1021 taaagaatat tcattaatgt agtttttccg gcgccgtttt ctcccaacaa tgccaatatc
     1081 tcgccttttt caagcgttaa attaatatgg tcattggcaa gaacacccgg aaattgtttg
     1141 gtaattccct ttaattcaag gattgggggc attttttctc ctttttggga tatgatttca
     1201 aaaaggctgt tttcaaaaac atccttcttt gtaaagatat ttttccgata tgaccggtta
     1261 aaaaaccgga tccctgaaaa acgattgaaa ttaattaaag caggtggagg aaaattcctc
     1321 caccttttca taatattctc taatttttaa ttttactgag cataaaccgg gttcaactca
     1381 atttcgccgg agataatctt tgcgcgaata tcatccagtt ctttcaccag gtcagccggt
     1441 actttatctt caaaatcgtg gaacggagca agatcaacgc ccttattttc cagcgttccg
     1501 acaactttac cgcctttgaa ggttccgtcg attacggatt cgattacctg catcgtagtg
     1561 gcatccatct tcttcataac cgaagtcagg ataatatcac cgttatccgg gtaagtcaga
     1621 taccaatcgg aatcgacacc gattaccata cccatcttcc ggtcttccat agcagcaatc
     1681 gttccgccgc ccaccggtcc ggcaaccggc ataataatat cggcgccctg atccatcatg
     1741 gtaatggata cctgttttcc tttatcaaga tcgtcgaaag aatcgacaca ggttccttca
     1801 agcgtttcgg tattccagcc caaaacttcg accttggtac cgtgaatttg attgtagtaa
     1861 tcggcgccgc gcgcaaaacc atccatgaaa atggttacgg tcggaatgca cattccgccg
     1921 aaagtaccga tgattccggt tttcgtcatc ccggcagcga cataaccggc aagaaaagcg
     1981 gcttcatccg tctggaaaac ctgatcaaca acattgggtt ttccgaccga tgcatcgata
     2041 atactgaatt tgaaatccgg attgtt
//
LOCUS       DAOMIV010000390         2065 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61404_length_2065_cov_3.201493, whole genome shotgun sequence.
ACCESSION   DAOMIV010000390 DAOMIV010000000
VERSION     DAOMIV010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61404_length_2065_cov_3.201493"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKV59_08330"
     CDS             complement(<1..477)
                     /locus_tag="PKV59_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQP46792.1"
                     /translation="MALFSRELGIDLGTLSLVIAEGKQLLLQEPTIAAIIVEEMKMVE
                     WGQAAKDMVGRVPESIEVVRPLRNGVIAEYEITENLLRFAIQKVSGSMLVFRPRILIT
                     IPCGITSVERRAVHEVGLGTNSREVLLMEQPLAAAIGVDLPIATPSGNMIICLGGGL"
     gene            complement(631..1062)
                     /gene="atpC"
                     /locus_tag="PKV59_08335"
     CDS             complement(631..1062)
                     /gene="atpC"
                     /locus_tag="PKV59_08335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HQP46793.1"
                     /translation="MNCQIVSRYNVVYEGPADLVLLPSVNGQLGILRNHVRMYTKLQP
                     GIITVRLAGEDTFYTATGGIAEVKPDVVRILVDSSENVDEIDVERALEAKKRAEEAMK
                     NAPSRNSTEFMRAMMEMKKSNLRISASQRRQMHRGTRNMQP"
     gene            complement(1075..>2065)
                     /gene="atpD"
                     /locus_tag="PKV59_08340"
     CDS             complement(1075..>2065)
                     /gene="atpD"
                     /locus_tag="PKV59_08340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HQP46794.1"
                     /translation="IDLIAPFTRGGKTGIFGGAGVGKTVVIMEFIRSIAAVHKGLSVF
                     AGVGERTREGTELLREMTDSGVMKDTVMVFGQMNEPPGVRLRVALAGLANAEYFRNQG
                     MDVLLFIDNIFRFSLAGSEVSALMGNMPSAVGYQPTLANEMGELQERITSTTLGSITS
                     MQAVYVPADDYSDPAPVTTFAHLDATIALERSIAEKAIYPAVDPLSSGSRILDPNIVG
                     EEHYKIAREVQRILQRYKDLQDIIAILGIDEISEEDKVIVNRARKAEQFFSQPMYVAE
                     RYTGIPGAYVRIEDTIEGFRQILSGELDDLPEQAFHMVGTIEEAKAKAQSMNQ"
BASE COUNT          549 a          546 c          407 g          563 t
ORIGIN      
        1 cagtccccca cccaaacaga taatcatatt tccagaggga gttgcgattg gcaaatcaac
       61 tccgatcgca gcagccaagg gctgctccat caataatact tcacgactgt tcgttcccag
      121 tcccacttca tgaaccgctc gccgttcgac actggttatt ccgcaaggaa ttgtgataag
      181 gatcctcgga cgaaacacca gcatagaacc gctgactttc tgaatggcaa aacgtaacag
      241 attttcggtg atttcatatt cggcaatcac accgtttcga agcggacgca cgacttcaat
      301 gctttcggga acacggccaa ccatatcctt tgcagcttgt ccccattcga ccattttcat
      361 ttcctcaaca atgatagctg cgatcgtggg ctcttgcaaa agaagttgtt tcccttcagc
      421 gatcaccaag cttaatgttc ctaaatctat tccaagttct cgagaaaaaa gcgccatatt
      481 aataattatc cagttctgaa aaatcggtat ttcatcttat ataatttata tcataaacca
      541 atgtttgata cggttgaaac taaataatat cggtcgggaa gtaaccaccg attttttcca
      601 tgcagcggat ctctgaaaaa agcggcgaca ttacggctgc atatttctgg taccgcgatg
      661 catttgcctt ctctggctgg cgctgatccg caggtttgat tttttcatct ccatcatagc
      721 gcgcataaat tcggttgaat tccgtgaagg agcatttttc atagcttctt ccgcccgttt
      781 ctttgcctct aaagcccgtt cgacatcaat ttcatcgaca ttttccgatg aatcaaccaa
      841 aatacgcaca acatccggtt taacttccgc aatgcctccg gtagcagtat aaaaggtatc
      901 ctcaccggcc aaacggactg ttataatacc gggttgaagt tttgtataca ttcgtacatg
      961 atttcttaat attcccagtt gtccgttgac actcggcaac aaaaccaaat ccgccgggcc
     1021 ttcatataca acgttatatc gcgaaacaat ctgacaattc actgtagtat cctattattg
     1081 attcatcgat tgcgctttgg ctttcgcttc ttcaatcgtg ccgaccatat gaaaggcttg
     1141 ttccgggaga tcatccaatt caccggataa tatttgtcga aagccttcta tcgtatcttc
     1201 gatacggaca tatgcaccgg gaattccggt ataccgttcg gcaacataca tcggttgaga
     1261 gaaaaactgt tcggccttcc tggcccggtt gacaatcact ttatcttctt ccgaaatttc
     1321 gtcaatcccc aaaatcgcga tgatatcctg cagatcctta taacgctgaa ggatccgttg
     1381 aacttccctg gcgattttat aatgctcttc accgacgata ttgggatcca aaattctgga
     1441 cccggacgaa agcggatcca ccgccggata aatcgctttt tcggcaatac tcctctccaa
     1501 cgcaatggtg gcatctaagt gggcaaaagt tgtcaccggt gccggatcag aataatcatc
     1561 ggccggcaca tagacagcct gcatcgaggt aatcgagcct aaggtagtcg atgtgatccg
     1621 ttcctgcagt tcgcccattt cattggccaa ggtcggttga taaccgacgg cggatggcat
     1681 atttcccatc aatgccgaaa cttcggagcc ggctaatgaa aaccgaaaaa tattatcgat
     1741 aaataagaga acatccatcc cttgattgcg aaaatattcc gcatttgcca aaccggctaa
     1801 agcgacccgc agccgaaccc ccggcggttc attcatttga ccgaaaacca ttacggtatc
     1861 tttcatgaca ccggaatcgg tcatttcacg caagagttcc gtcccctcgc gggttctttc
     1921 tccgactcct gcaaagaccg ataacccttt gtggactgcg gcgatacttc gaataaattc
     1981 catgatcact acggtctttc ctacgcccgc tccgccaaaa atccctgttt tcccgcctcg
     2041 ggtaaacggg gcaattaaat caatt
//
LOCUS       DAOMIV010000391         2064 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61479_length_2064_cov_2.740169, whole genome shotgun sequence.
ACCESSION   DAOMIV010000391 DAOMIV010000000
VERSION     DAOMIV010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61479_length_2064_cov_2.740169"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PKV59_08345"
     CDS             complement(<1..900)
                     /locus_tag="PKV59_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46795.1"
                     /translation="MMEPSNNTTKSEKHAVEIKKPKKSSNSRFLYDLKKSTKAKFAII
                     VIFIVSLFAILAPILTPYDPVTTDLMARLKPPVPFRYSVVSHPLGTDALGRDIWARIV
                     YGARVSLLVGITSVIISGCIGVTLGLIAGYFGGIVDDIIMRIADILLAFPFILLMLAV
                     LAVIGPGLGNMILVLGLTGWVSYARVVRGEVLSLREKEFVESARSLGASNMRIIFKHI
                     LPNVVNSVIVVASFAVASTIITEATLSFLGLGVKPTTPTWGMMLSEGREYIRQAWWLT
                     TLPGIAIGITVLGINLLGDWLRDV"
     gene            complement(924..1850)
                     /locus_tag="PKV59_08350"
     CDS             complement(924..1850)
                     /locus_tag="PKV59_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46796.1"
                     /translation="MARYTARRLLQAIIVIFGITIAAFSINFLAGDPTYVLIGDMRGM
                     TEEDIQAFRHRMGFDRPILVQYFDWLKNAVRGDFGWSYKYKEDNWKIIVERFPATLQL
                     TVVSLILQLLVSFPLGVFAALKRGTKWDSFSMVLALLGQSMPSFWIGLMLMMIFSVNL
                     KWLPVSGRYEGIRSMVLPVITLSFYGIARNTRLIRSSMLEVLGEDYIRTARAKGQTER
                     NVYIKHALRNVLIPVVTMVGMDLGSLLSGALVVETIFAWPGMGMLTVNAINGKDIMMV
                     QACVTLFALMFVFANLLVDFTYTILDPRVRLQ"
BASE COUNT          650 a          513 c          365 g          536 t
ORIGIN      
        1 aacatctcgc aaccaatctc ccaacaggtt aattcctaaa acagtaattc caatcgctat
       61 cccgggtaaa gtagtaagcc accaagcttg ccggatatat tcacgaccct ctgaaagcat
      121 catcccccaa gttggcgttg tcggtttaac tcctaacccc aaaaaactca acgtggcttc
      181 ggtaataatt gtcgaagcaa cagcaaatga tgcaaccaca atgacggaat taacgacgtt
      241 cggcaaaata tgcttgaaaa taatccgcat attacttgca ccaagcgagc gtgccgactc
      301 aacaaattct ttttcacgca aagaaagtac ttctcccctg acaacccgag catatgaaac
      361 ccatccggtc aagcccaaga ccaaaatcat gtttcccaat ccgggaccga tcacagcaag
      421 gacagccaac atcagtaaaa taaagggaaa agccaataga atatcagcta tgcgcataat
      481 gatatcatcg actatcccgc caaaatatcc ggcgattaat ccgagtgtta ccccgatgca
      541 tccggaaata atgaccgacg ttattccgac gagcaaggat acccgtgctc cgtaaacgat
      601 ccgagcccaa atatctcttc ccaatgcgtc tgttccaagc ggatgactca caaccgaata
      661 acggaacggt acggggggtt tcagccgtgc cattaaatca gtggttaccg ggtcatatgg
      721 cgttaatatc ggagcgagaa tagcaaataa cgaaacgata aaaattacga tgatcgcaaa
      781 ttttgctttc gtcgattttt tcaaatcata caaaaaacgg ctgttactcg atttttttgg
      841 ctttttaatt tctaccgcat gcttttcaga ttttgttgta ttgttagatg gttccatcat
      901 attatttcct acttatcctt tggttattga agtctcacgc gggggtcaag tattgtgtag
      961 gtaaaatcaa ccagtaaatt cgcaaaaacg aacatcaatg caaaaagtgt cacacaagct
     1021 tggaccatca taatatcttt cccatttatg gcattgacgg tcagcattcc catcccgggc
     1081 catgcaaata ttgtttcgac aaccaaagct ccgctgagca ggcttcccaa atccatgccc
     1141 accattgtca caaccggaat caacacattt cttaaggcat gttttatata aacatttctt
     1201 tccgtttgcc cttttgcacg cgcagtccga atataatcct ctcccaaaac ttccagcata
     1261 gaacttctga tcaatcttgt atttctcgca atcccataaa atgacagcgt aattaccggg
     1321 aggaccatac tccgaattcc ctcatatcgc cccgaaaccg gaagccattt tagattcacg
     1381 gaaaatatca tcatgagcat taacccgatc caaaaagacg gcatcgactg gccgagtaag
     1441 gccagaacca tagaaaagct atcccatttt gtcccccgct ttagggcagc aaatacccca
     1501 agcgggaaag acaccaataa ttgcaaaatc aatgaaacca cagtgagttg cagcgtagcg
     1561 ggaaatcgct cgacaatgat tttccagttg tcctctttat atttataaga ccaaccgaaa
     1621 tcgcctcgga cagcattttt taaccagtca aaatattgga ctaaaatcgg acgatcaaac
     1681 cccatacgat gtcgaaatgc ttggatatct tcttccgtca tcccgcgcat atcaccgatt
     1741 aatacataag tcggatcacc cgctaaaaaa ttgattgaaa atgccgcaat tgttataccg
     1801 aaaataacta taatcgcttg aagcaaacgc cgagctgtat atcgagccac tattacctcc
     1861 taacaagctg cagaaagtta tcgctggatt tagatttcca aatttttacc actattcact
     1921 tatctcgaaa aatgatcctt cgaagtaagg cagtgaaata tgctcactgc cttactcgaa
     1981 tgtttatagc gtaaataata ttattttgtt gatccggaaa aagttactta tattttttcg
     2041 cagatttcat cagcgcacga ccgg
//
LOCUS       DAOMIV010000392         2063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61498_length_2063_cov_3.958167, whole genome shotgun sequence.
ACCESSION   DAOMIV010000392 DAOMIV010000000
VERSION     DAOMIV010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61498_length_2063_cov_3.958167"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKV59_08355"
     CDS             <1..818
                     /locus_tag="PKV59_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQP46797.1"
                     /translation="GIIKRNVPLIAAAQDPKAMEVIRKIAEERNAEWIDVDQNYGFVP
                     LKDSVDGQRLMIWERKEQNALRNWLENREKSIWKPWEVDIPLAGYHQSINAVIAFTAL
                     MKIRSFADKINEKSISDGLAKTFWPCRFEKVSIDPPVILDGAHNPDSIEKLVMTLNRF
                     YGTKRITCVFGCSEDKDLSGMIRILAPFVSDFIVTRSTHPRSMETKQIYDEVLSNGRP
                     AVIRESLELALRDIESGDKNNVYIVTGSLFVTAGIRELLMKRNPDMKYFEKKK"
     gene            833..>2063
                     /locus_tag="PKV59_08360"
     CDS             833..>2063
                     /locus_tag="PKV59_08360"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HQP46798.1"
                     /translation="MSSSNWLEDKDLVNDFTTNINQRTEELFRIPNMELDSDGCFVAP
                     VVVLRENVIFPKMITPVFVGQQKNLESLRYGLENEQTVITLIPKDPEKEFPVKKSDFY
                     PVGLEIAVGRLVNLPEEHFSTLVQGRRRVILMDIVRRSPVLMARVYVSEAEAKKSRHL
                     EALIRTTRNLFEKCVQLDRKLPDEAVAYASTITDPSWLADMIATTISFENEQRQEILF
                     ELDPEKRLEILNSFLAQELDVLELGNQISTTVQNEVDKNQREFYLREQMRAIQKELNE
                     DDVFSRDLNELKEKIDQKQFSDEAREVALKEFERLTQLPAMSPEVNVSRSYIDWLLDL
                     PWVEQTEDNLDVKHASEILNKNHYGLEKAKDRILEYIAVRSLKPKKEKQPILCFVGPP
                     GTGKTSIGRSIAEALGKK"
BASE COUNT          663 a          345 c          498 g          557 t
ORIGIN      
        1 gcggcattat taagcggaat gttcctttaa tcgcagctgc tcaagatccg aaagctatgg
       61 aagtgatccg aaaaatagcc gaagaaagaa atgccgaatg gattgatgtg gatcaaaatt
      121 atggctttgt tccattaaag gattccgtgg acggacagag actgatgatc tgggagcgta
      181 aggaacaaaa tgcattgcgg aattggctgg aaaatagaga aaagagtata tggaaacctt
      241 gggaagttga tattccgctt gcgggatatc atcaatctat caatgccgtg atcgcattca
      301 cagcgttaat gaaaatcaga tcttttgcgg ataaaataaa tgagaaaagt atcagcgatg
      361 gattagcgaa gactttttgg ccatgcaggt ttgagaaggt atcgattgat ccgccggtta
      421 ttttagacgg ggcgcataat ccggattcga ttgaaaaact tgtcatgaca ttgaatcgtt
      481 tttatgggac aaagaggata acctgtgttt tcggttgttc cgaggataaa gatttaagcg
      541 gaatgatcag gatacttgcg ccgtttgtca gtgattttat tgttacccgt tcaactcatc
      601 caagatcaat ggaaacaaaa caaatttatg atgaagtgct ttcaaacggc aggccggctg
      661 ttattcggga atcattggaa ctcgctttac gagatatcga aagcggtgat aaaaataatg
      721 tttatattgt tacaggcagc ctttttgtta cggcggggat tcgtgaactt ttgatgaagc
      781 gtaatcccga tatgaagtat tttgaaaaga aaaaataaca tttggagttt aaatgagttc
      841 aagtaattgg ttagaagata aagatcttgt caatgatttt acgacgaata tcaatcaaag
      901 gacggaggaa ctgtttcgaa ttccgaatat ggaattggat tcggatggtt gttttgtcgc
      961 gccggttgtt gttttaagag aaaatgtgat ttttccaaaa atgataacgc cggtattcgt
     1021 cggccaacaa aaaaatcttg aatccttacg atatggttta gaaaatgaac aaaccgtaat
     1081 tacattaatt ccaaaggatc cggaaaagga atttccggtc aagaaatccg atttttatcc
     1141 ggtcggactg gagattgcgg ttggacgtct agtcaattta cctgaagaac atttcagtac
     1201 gttagttcaa ggcagacggc gggtcatact gatggatatt gtccggcgat cgccggtcct
     1261 tatggcacgg gtttatgtat cggaagcaga agcaaagaaa tccagacatc tggaggcgtt
     1321 gatccggact acccggaacc tttttgaaaa atgtgtccaa ttggatcgca aattgccgga
     1381 tgaagcggtc gcttatgctt ccacaattac tgatccgtca tggttggctg atatgatcgc
     1441 aaccaccatt tcttttgaga acgaacaaag acaggaaatt ctttttgaat tggatccgga
     1501 aaagcgactt gaaatcttaa acagctttct ggcacaagaa ttggatgtat tggaactcgg
     1561 aaaccaaatc agtacaacgg ttcagaatga agtggataaa aatcaacgtg agttttatct
     1621 gcgggaacaa atgagggcaa ttcaaaaaga attaaatgaa gatgatgtct tcagccgtga
     1681 cttaaatgaa ctgaaagaga agattgatca aaaacaattc agtgacgagg cacgtgaagt
     1741 cgccctcaaa gaatttgaaa gattgacaca acttccggca atgtcccctg aggtcaatgt
     1801 atccagaagt tatattgact ggcttttaga tttaccgtgg gtggaacaga cggaagataa
     1861 cctggatgtg aaacatgctt cggaaattct gaacaagaat cattacggtt tggaaaaagc
     1921 gaaagatcgg atattggaat atattgcggt gcgttcctta aagccgaaga aagagaaaca
     1981 acccatttta tgctttgtcg gccctcccgg aacgggaaaa acatcaatcg gtcgatctat
     2041 cgcagaagcg ttaggaaaaa agt
//
LOCUS       DAOMIV010000393         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61598_length_2061_cov_4.055334, whole genome shotgun sequence.
ACCESSION   DAOMIV010000393 DAOMIV010000000
VERSION     DAOMIV010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61598_length_2061_cov_4.055334"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..>2061
                     /gene="glgP"
                     /locus_tag="PKV59_08365"
     CDS             101..>2061
                     /gene="glgP"
                     /locus_tag="PKV59_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQP46799.1"
                     /translation="MKRVIPIASSLPKRIERLNDLAYNLWWSWNPEAINVFSKIHPVL
                     WDSVSHNPVAFLQQVSRTLLNRVANDKQYLDLYDRVVNNFDSYMGEKETWFVKNYPEV
                     TAGQSIAYFSFEFGLHESIPAYAGGLGILAGDHLKESSDMGIPLEAIGFIYNQGYFVQ
                     KITEDGWQETSNFYLDFNKLPMIPILDEKDKPILISVQLPGREIKARIWQIQVGRVPL
                     YLLDTNISENSSFDQQLTAKLYTSDLETRISQEILLGMGGVRVMRALGYNPTVWHMNE
                     GHSAFLSVERCAELVRQGKTFEEAKEIVRKGNVFTTHTPVPAGNDKFPVWLVEKYFSQ
                     IWPDLGLTREQFIDLAKEASPYGDQFTMPILALRLSERCNAVSELHGQVSRNMWHFLW
                     PDRKVEEVPITHITNGVHTFSWLSRRMGILFDEYLGTEWRLDPSNPKVWEKVDSIPDA
                     ELWAVKRHQKRKLMRFINDKARAQWLTGEVHPVQTIAAGVLLDADALTIGFARRFPTY
                     KRGYLILRDYERLLRIINNVDAPVQIIFSGKAHPADEPGKLIVQQLYRAIKDHRTGGR
                     MVFVENYDMDIARHLVQGVDVWLNTPRRPMEASGTSGMKAAMNGCLNFSVLDGWWHEG
                     YNGKNGWAIGDDKTYESDEKQDEMDALSL"
BASE COUNT          545 a          408 c          533 g          575 t
ORIGIN      
        1 gtatcattat ctgcggattg ccctttatta attcggcatc gagtgctgaa cgggcatccc
       61 gttaaataat tgaataggga aaataataat gtctataaat atgaaaagag tcataccgat
      121 tgcaagcagc cttccgaaga gaatcgaacg tttaaatgat ttagcttata acctgtggtg
      181 gtcgtggaat ccggaagcaa taaacgtttt cagcaaaatt catccggttt tatgggattc
      241 cgtctcacat aatcctgtgg catttctgca gcaggtttcc cgaaccttat tgaatcgagt
      301 cgccaatgac aagcaatacc tcgatctata tgatcgcgtg gtgaacaatt ttgattccta
      361 tatgggcgaa aaagaaacat ggtttgtaaa aaactacccg gaagtgactg ccggtcaatc
      421 catcgcttat ttttcattcg aattcggttt acatgaatcc attccggctt atgccggtgg
      481 tttgggaatt ctggccggtg atcatctgaa agaatccagt gatatgggga ttcctttgga
      541 agcgatcgga tttatttata accagggtta ttttgtccaa aaaatcaccg aggatggttg
      601 gcaggaaaca tcgaattttt atcttgattt taataagctg ccgatgatcc cgattttgga
      661 tgaaaaagat aaaccgattc tgatatccgt tcagcttccg gggcgtgaaa taaaagcacg
      721 gatttggcag attcaagtcg gccgggtccc tctttatttg ttggatacca atatttccga
      781 gaattcatcg tttgatcaac aattgaccgc gaaattatat acttcggatc ttgagacacg
      841 gatttctcag gaaattctgc tcggaatggg cggcgtgcgg gtaatgcgcg cattgggtta
      901 taacccgacc gtttggcata tgaatgaagg tcattccgca ttcctatccg ttgagaggtg
      961 tgcggaatta gtacgtcaag ggaagacttt tgaagaagct aaagaaatcg tgcgcaaagg
     1021 caacgttttt acgacccata ccccggttcc cgcgggaaat gacaaattcc cggtttggtt
     1081 ggtagagaaa tatttcagtc agatttggcc cgatttgggt ttgactcgcg agcaattcat
     1141 tgacttggca aaagaagcca gcccttacgg agatcaattt accatgccga ttcttgcgct
     1201 gagactttcg gagcggtgca atgcggtgtc ggaattacac ggtcaggtat cgcgcaatat
     1261 gtggcatttt ctgtggccgg atcgaaaggt ggaagaggtt ccgattacgc atatcacgaa
     1321 cggtgttcat accttcagtt ggctttcacg ccgaatgggt attttatttg atgagtatct
     1381 cggtaccgaa tggcgactcg atccttccaa tccgaaggtt tgggaaaaag tggattccat
     1441 tccggatgcc gaactctggg cggttaaacg tcatcagaag cgtaagctga tgcgctttat
     1501 caatgataaa gcgcgagcgc aatggctgac cggtgaagtt catccggtac aaaccattgc
     1561 ggccggggta ttacttgatg cggatgcgtt gacaatcggt tttgccagac gatttccgac
     1621 ctataagcgg ggttatctta tcctgcggga ttatgaaaga cttttacgga ttatcaataa
     1681 tgtagatgct ccggttcaga ttatcttctc cgggaaagct catccggcgg atgagcccgg
     1741 aaaactgatc gttcaacaac tttatcgggc gatcaaggat catcggaccg gcggtcgaat
     1801 ggttttcgtc gagaattatg atatggacat tgcccgtcac ctggttcagg gagtggatgt
     1861 ttggttgaat actccccgtc gtccgatgga agcatcggga acttccggga tgaaggcggc
     1921 tatgaatggt tgtttaaact tctccgtttt ggacggttgg tggcatgaag gatataacgg
     1981 gaaaaacgga tgggcgatcg gcgacgataa gacctatgag agtgatgaga aacaggatga
     2041 aatggatgcg ttgagtttat a
//
LOCUS       DAOMIV010000394         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61601_length_2061_cov_3.839980, whole genome shotgun sequence.
ACCESSION   DAOMIV010000394 DAOMIV010000000
VERSION     DAOMIV010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61601_length_2061_cov_3.839980"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="PKV59_08370"
     CDS             <1..818
                     /locus_tag="PKV59_08370"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQP46800.1"
                     /translation="DLTSSHTAAQRWLPPKGVSDSGQLKGMIAIDVFGQPADFDALRT
                     ITDPFQLKVIEDSCEALGAEYKNRKAGTLGDFGVFAFYPNKQITTGEGGLVITNHDED
                     ADKIRAMRNQGRAPGDTWLEHTYLGYNYRMDELSAALGAAQMKRIRELMAARQRVADW
                     YRAELSSVPGITCPVVAPTTTRSSWFVYVIRLDRGIDRKSLSEKLEALGIPVRPYFSP
                     IHFQPYIREKFGYQKGRFPVTEDLGERGLALPFSGKMTEEQVQEIASALRQIL"
     gene            889..1740
                     /locus_tag="PKV59_08375"
     CDS             889..1740
                     /locus_tag="PKV59_08375"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQP46801.1"
                     /translation="MGLIVLVSLFLLFLGISIFGFTFFPRQEPGPVFFLSFILSILFL
                     VPVAFILYRCYGLLSSSYTLTRDRLQIKWGLRQEIIPLSEINWVHTPQEMPEEIPWPF
                     LPMPGAYLGKVHTASGETIEFMASDTKKMIFVGTSKGVYGISPADPKEFLDGFQRVLQ
                     MGSVFEPTHQTIRPADWIAASWENHIARRSFVFSAVFLVLMFLWVGFHFSGLENSRIN
                     STNETAFPIRNILILPFLAGVFWVIDMGIGVRLYKSDKLKRIAELIWSSGGIITFLFF
                     IAGFIIK"
     gene            1780..>2061
                     /locus_tag="PKV59_08380"
     CDS             1780..>2061
                     /locus_tag="PKV59_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46802.1"
                     /translation="MVKSGERKNILPLILLFIFFAIYGIYLFFAFAQVPMDSDFASLV
                     LEANDILNGNILLKGWHLTNAPFTFTEIPFYVLGSAFFGINVWSYIFANM"
BASE COUNT          520 a          426 c          459 g          656 t
ORIGIN      
        1 atgatttgac ttcttctcat acagccgcac aaagatggtt acctccgaaa ggagtatccg
       61 attcggggca gttgaaaggc atgatcgcga tcgatgtatt cggccaaccg gctgattttg
      121 atgcattgag aacaatcacc gatccgtttc agttgaaagt catcgaagat tcttgcgaag
      181 cattgggcgc cgaatacaaa aatcgaaaag cggggacact gggagatttc ggagtcttcg
      241 cgttttatcc caataaacaa atcactacgg gagaaggcgg tttggtaatt accaaccatg
      301 acgaagatgc cgataaaatc cgcgcaatgc ggaatcaggg acgcgctccg ggagacactt
      361 ggttagaaca tacctattta ggatataact accgtatgga tgaattaagt gccgcactgg
      421 gagcagccca gatgaagcga atccgggaac tgatggccgc ccgccagcgg gttgccgatt
      481 ggtaccgggc tgaactttct tctgtccccg gaattacctg tccggtggtt gcccctacca
      541 ccacccgcag cagttggttc gtttatgtga tccggttgga ccgtgggatc gatcgaaaat
      601 ccctatcgga aaaattagag gcattgggaa ttccggttcg tccttatttt tcacctatcc
      661 attttcaacc ctatattcga gaaaaattcg ggtatcagaa aggcagattt ccggttacgg
      721 aagatctggg agaaagggga ttggcattac cattttccgg aaagatgacc gaagaacaag
      781 tacaggaaat cgcatccgct ttgcgacaaa tcctgtaatt ctctattcct aaagatgaaa
      841 ccctcgaaga aaaaaacgaa tgaattcatt ttctttcccg atcgattttt ggggttaatt
      901 gtcctggttt ctttattttt attgtttttg ggaatcagta ttttcgggtt cactttcttt
      961 ccgcgacagg aacccggtcc ggtttttttt ctgtctttta ttctttcgat tttatttttg
     1021 gttccggttg cttttatttt gtatcggtgt tatggtttgc tttcttcttc ctacacatta
     1081 acccgagatc gactacaaat caagtgggga ttacggcagg aaattattcc tctatccgaa
     1141 atcaactggg tccatacgcc ccaagaaatg ccggaagaaa ttccctggcc atttttaccg
     1201 atgcccggtg cctacctcgg aaaagtccat accgcttcgg gtgaaacaat cgagtttatg
     1261 gcttccgata caaagaaaat gatatttgtg ggaacatcaa aaggcgttta tggtatttct
     1321 ccggctgacc caaaggaatt tttggacggc ttccaacgtg tgctgcaaat gggatctgtt
     1381 tttgaaccca ctcatcaaac cattcgaccc gcggattgga ttgccgcttc ttgggaaaat
     1441 catatcgcca gacgttcttt tgttttttca gctgtttttc ttgtcctgat gtttctttgg
     1501 gttggttttc atttttccgg tttggaaaat tcccgcatca attccacgaa tgaaacggca
     1561 tttccgatac gcaatattct gattcttccg ttccttgccg gagttttctg ggttatcgat
     1621 atgggcatcg gtgttcggct ttataaatcg gataaattga aacggatagc tgaactgatc
     1681 tggagcagcg gtggaatcat tactttttta ttctttatag cgggatttat tataaaatag
     1741 ttttctaaat aatcattgat gatggagaaa aataaattta tggtaaaaag cggagagaga
     1801 aagaatattt tacccctcat ccttttattc atttttttcg ctatttacgg gatttatctt
     1861 ttctttgcat ttgcacaagt gccgatggat tccgattttg cctctctggt gttggaagca
     1921 aatgatattc ttaatgggaa tattctttta aaaggttggc atcttacgaa tgcacctttc
     1981 acttttaccg agatcccttt ttatgtgctt ggcagtgctt ttttcggtat caatgtctgg
     2041 tcatatattt ttgcaaatat g
//
LOCUS       DAOMIV010000395         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61857_length_2056_cov_4.064468, whole genome shotgun sequence.
ACCESSION   DAOMIV010000395 DAOMIV010000000
VERSION     DAOMIV010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61857_length_2056_cov_4.064468"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="PKV59_08385"
     CDS             <1..1286
                     /locus_tag="PKV59_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46803.1"
                     /translation="RSATLPFIIQKLNPDLKYELTDVSEPFFGSEAELQVQPGTEKLV
                     IFQTLLSIFSWCFFTLTVVGLFRHELSKIFAAIILFGFAFVPQIADWDSILLSESMSF
                     SFFILLCAFLIRFLQNCYGPGSLMRRFLSGFFLLISAIFWLFTRDTNAYFIAFLSVIF
                     FILFCITVFSHHKFQGLMLVLSLCLAACFLFQQQTFRTSKRWLLPLLNNLTTNVFPYP
                     DRVAFFEEKGMPVNEAVLSQSGSAEYNDLYNQKEFIQWAETNGLSAYTAFLMNMPLWS
                     VQQIFLNLDGFFAENLQPFFYEKPDEKPHWADKVGNVLHPLSSAVLWIDLFLLLILFI
                     YTIRKQDADSFVWFLFSFLLTIGSIMMISVAYLGEVRSVWRHVLCGVMPLRLILWIQI
                     LEILDQSLDSRPSPPIINRLGGQSNRSETDKILDR"
     gene            1323..>2056
                     /gene="tpiA"
                     /locus_tag="PKV59_08390"
     CDS             1323..>2056
                     /gene="tpiA"
                     /locus_tag="PKV59_08390"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQP46804.1"
                     /translation="MRRPMVAGNWKMNKTAAEAIILVQELLPELQKQTAIDRVICPPF
                     TALYPVSGLLKGSGIGCGAQDVFWEEKGAYTGEIAPNMVAELGEYVIIGHSERRTYFY
                     ETDETVNKKTLAALAAGLTPIVCVGETLDENQSGRTKEVVSRQVREGLKGIPEMYVEK
                     IVIAYEPVWAIGTGLAATGKDANRVIAEDIRKPYADLYGEKAAQSIRVLYGGSVKGNN
                     AAEFFGQPDIDGALVGGASLKAPDFM"
BASE COUNT          517 a          407 c          497 g          635 t
ORIGIN      
        1 atcgttcggc taccctgcct tttatcattc aaaaactcaa tccggatctc aaatatgaac
       61 taaccgacgt ttcggagcct tttttcggat ccgaagcgga attacaagtt cagcccggga
      121 cggagaagct cgttattttt cagacccttt tgtccatttt cagttggtgt tttttcactc
      181 tgactgttgt cggattgttc cgtcacgaac tgagcaaaat cttcgcggcc attattttat
      241 tcggattcgc ttttgttccg caaatcgccg actgggacag catcctatta tccgaatcca
      301 tgtctttttc tttttttatt ctgctgtgtg cgttcttaat acggttctta cagaattgtt
      361 acggtcccgg aagtttaatg cgcagatttt tatcgggctt tttcctgctg atttcggcaa
      421 ttttttggct atttacacgg gataccaatg cctattttat cgccttcttg tccgtgatat
      481 ttttcattct gttttgtatt accgtattca gccatcataa attccagggg ttgatgttgg
      541 ttttgtctct gtgcttggcg gcttgttttt tgttccaaca gcaaaccttt cgcaccagca
      601 aacgatggct gttaccgtta ttaaataacc tgacaaccaa tgtttttccc tatccggacc
      661 gtgtcgcatt tttcgaagaa aagggaatgc cggtcaatga ggctgttctt tctcaatccg
      721 gttcggcaga gtataacgat ttatacaatc aaaaagaatt tattcaatgg gctgaaacga
      781 acggtctttc ggcgtatacc gcgtttctga tgaatatgcc gttatggagt gttcagcaaa
      841 tctttttaaa cttagatgga ttttttgccg aaaatcttca gccgtttttt tacgagaaac
      901 cggatgaaaa gccccattgg gcggataagg tcggaaatgt tttgcatccg ttatcttccg
      961 ccgtcctctg gatcgatctt ttcctattgt tgatattgtt tatttataca atccgaaaac
     1021 aggatgccga ttcgtttgtt tggtttttgt tttcattttt gttgacaatc ggaagcatta
     1081 tgatgatttc ggttgcttat ttaggcgagg tgcgttccgt gtggaggcat gttctttgcg
     1141 gagtgatgcc gttgcgattg attctttgga tccaaatttt ggaaatattg gatcagtcac
     1201 ttgattcccg gccttctccc cctatcatca accgcctggg aggacagtcg aatcgttcgg
     1261 aaactgataa aattctggat agataaagaa taatttagga tattgacagg agtgaaataa
     1321 caatgagaag accaatggtt gcaggcaact ggaagatgaa taaaaccgct gcggaagcaa
     1381 ttatcctcgt tcaggagtta ttacccgagt tacagaaaca aaccgctatc gatcgtgtaa
     1441 tatgcccgcc gtttaccgct ttatatccgg tttccggact tttgaaggga agcggtatcg
     1501 gatgtggtgc tcaggatgtg ttctgggaag aaaagggagc ctataccggg gaaatagcgc
     1561 cgaatatggt tgccgaactg ggtgagtatg taattatcgg acatagcgaa cgacggactt
     1621 atttttatga aacggatgag acagtcaata aaaagacgct tgcagcctta gccgccggat
     1681 tgacgccgat tgtctgtgtc ggagaaacat tggatgaaaa ccaatccgga aggacgaaag
     1741 aagtggtttc acgacaggtt cgcgaagggt tgaaaggaat tcccgaaatg tatgtcgaaa
     1801 aaatcgtgat tgcttatgaa ccggtatggg caatcgggac gggtttggcg gcaaccggga
     1861 aagacgcaaa ccgagtgatt gcggaagata ttcggaaacc gtatgccgat ctttacggag
     1921 aaaaagcggc acaatcgatt cgggtgctgt atggcggatc ggtaaaagga aataacgccg
     1981 cagaattttt tggacagccc gatattgacg gagctttggt aggcggagct agtttgaaag
     2041 cgcctgattt tatgga
//
LOCUS       DAOMIV010000396         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61858_length_2056_cov_3.828086, whole genome shotgun sequence.
ACCESSION   DAOMIV010000396 DAOMIV010000000
VERSION     DAOMIV010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61858_length_2056_cov_3.828086"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1935)
                     /locus_tag="PKV59_08395"
     CDS             complement(37..1935)
                     /locus_tag="PKV59_08395"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HQP46805.1"
                     /translation="MKKLNLLIIFAIPFFLLFSHAHGEESISNASSAEEMQNRLESIQ
                     IDPADREEIARRFHHLTGELSPIPEPLNWQVGMDHLFWVINTDRKITVQIDAEAVYVG
                     EHIVIWVEKTALSQLSDAVYDQFKKFDFEIYPFERSVFGSELSPGIDHDLRIHALFTK
                     KAGAGILGYFSSRDEDHPQVSPHSNAMELFILDSTMLAGNPDQITNTLSHEFQHMIHY
                     AHDANEGSNIDEGFSGLAEYLIHNRLSEAYERSFLENPDISLTLWPVSGSSIPYYGAS
                     FLFSKYLADRLGQDFLNKVVEQPRNGFEGIDAALKEVKSTFTADQLFAEWTAANLLYG
                     LGISNGEFSYRDYEPPLPKNDSMIKSLSCKNQSFDSTAMQYGTDYYNLPCDAGTYEIE
                     ITGQPTIPILSMNPIQGQYAWWSNSVNNSDTWMQHNFDLSNAKGSIRFEFDLNYDLEE
                     AYDFLYLSLSADGGKTWQMLKTAHGTDLNESGFNLGWGWTGKSGGWFHESVNLSEYSG
                     KNVLLRFDLITDQALTGEGVLLDNFRIDAIDFSDDVEKGSSGWEANGFLPIMNQLPQQ
                     YSDVLITGDSKKPAIFFHTTEGSETFSWTCDFEQQDKPICAFGISPINRISQLSAAYR
                     IQIKRNGP"
BASE COUNT          603 a          456 c          422 g          575 t
ORIGIN      
        1 ataaaataac ggatcatgat ctgtttttga aacgattcat ggtccgtttc tttttatctg
       61 tattcgatat gcggctgata attgagatat tcgattgatc ggactaatac cgaacgcaca
      121 aattggttta tcttgctgct cgaaatcaca tgtccatgaa aatgtttcgg aaccttccgt
      181 tgtatgaaag aaaatcgcag gttttttcga atcgccggtg ataagaacat cggaatactg
      241 ctggggaagc tgattcatga tcggcaggaa gccgtttgct tcccaaccgg aacttccttt
      301 ttcaacatca tccgaaaaat caatcgcatc aattctgaaa ttatccagca gcaccccttc
      361 gccggtcaat gcctgatccg taatcaggtc aaagcgcaaa aggacatttt ttccggaata
      421 ttcgctgaga ttgacggatt catgaaacca tccgccgctt ttccccgtcc atccccaacc
      481 gaggttgaat ccggattcgt ttaagtcggt cccatgggct gttttcaaca tctgccaagt
      541 ttttccgcca tcggcagaca gagaaagata gagaaaatca taagcctctt ccaagtcata
      601 atttagatca aattcaaagc gaatcgaacc ttttgcattt gataaatcaa aattgtgttg
      661 catccatgta tccgagttgt tgactgaatt actccaccat gcatactgac cttgaatagg
      721 attcatcgat aaaatcggga tggtaggttg gccggtaatt tctatttcat aggtccccgc
      781 atcgcatgga agattgtaat aatccgtccc gtattgcatc gcggtcgaat caaaagattg
      841 gtttttacaa gacaaactct ttatcataga atcgtttttc ggtaacgggg gttcataatc
      901 ccggtaagaa aattctccgt ttgagatacc caaaccatac aaaagattgg ccgccgtcca
      961 ttccgcaaaa agttgatcgg cagtaaaagt gctcttcact tctttcaacg cggcatcgat
     1021 tccctcaaaa ccgtttcgcg gttgttctac tactttattc agaaagtcct gccccagtcg
     1081 atctgcaagg tacttcgaaa aaagaaaaga agcgccataa tacgggatac ttgaaccgga
     1141 aaccggccat aacgtcaggg aaatatccgg attttccaaa aaagatcgct cataggcttc
     1201 ggataatcga ttatgaatca gatattcggc caatcccgaa aatccttcgt caatattgga
     1261 cccttcattg gcgtcatgcg cataatggat catgtgctga aattcatgcg acagcgtatt
     1321 ggtaatctgg tcagggtttc ccgccagcat cgtcgagtcc aatataaaga gttccatcgc
     1381 gttggaatga ggagaaacct ggggatgatc ttcatcccgg gaagaaaaat aaccaaggat
     1441 tccggctccg gcttttttgg tgaaaagagc atgaatccgc aaatcatgat caatcccggg
     1501 agaaagctcc gacccgaaca cggatctttc aaaaggataa atttcaaagt caaatttctt
     1561 gaattgatca tagactgcgt cggaaagttg agaaagtgcc gttttttcta cccatatgac
     1621 gatatgttct cccacataaa cggcttccgc atcgatttga acagtaattt tacgatcggt
     1681 attaattacc caaaaaagat gatccatacc gacttgccaa tttaatggtt ctggaatcgg
     1741 ggagagttcc ccggttaagt gatgaaatcg ccttgcaatc tcttctctgt ctgctggatc
     1801 gatttgaatt gattccaagc ggttttgcat ctcttcggca gaagatgcat tggatatgga
     1861 ttcttcgcca tgagcatggg agaataaaag aaaaaatgga attgcaaata ttatcaaaag
     1921 atttaacttc ttcatgaagc gtgctgctct tttaaattat gcttttcttc aaagtttccg
     1981 ttcgggatcg gtatcaagga cacacctccg accaacccca tagaaaccca taaatagggc
     2041 aaagcaaatg tatcca
//
LOCUS       DAOMIV010000397         2053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_61994_length_2053_cov_4.373874, whole genome shotgun sequence.
ACCESSION   DAOMIV010000397 DAOMIV010000000
VERSION     DAOMIV010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61994_length_2053_cov_4.373874"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(340..1890)
                     /locus_tag="PKV59_08400"
     CDS             complement(340..1890)
                     /locus_tag="PKV59_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46806.1"
                     /translation="MYKRKILFVFIFLTVFAFAAQGLLTVSAQSDNAAKAPDQDLVIA
                     FNNDIRALDPHNVSDTLTISATRTMYESLVKFNEKQEIVPLLAESYEISDDGMTYTFH
                     LRSGVLFHDGTEFNSDAVKANIDRCKDESLRQNRIVKQIANAEYPDPYTVILTLAKPN
                     NTFLNKMTMFDFIAPATIAAGEEKILREPCGTGPYTFKDRVEGDHVTVVPFEKYWDGA
                     PQVASLTFKAVPEDGARVAMLQTGEADYIYPMPTTQIKVVEGKKDILIDAKTSNIMRY
                     VTLNTELEQLKDKRVRQAINYAVDKDAYIKTVFNGYASKVYSCYPETIQYYAPQPPYD
                     FNLEKAKALMKEAGAENGFKLTIWGDNITSEEKGMQFIQQQLKQIGIDVEVMPMEANV
                     VAEKIQAERDKAEVNMWYVNWSASSFDADGAIRAILHGESIPPGQYNSAYYNNEEVNK
                     LMDEALAITDPKILQEKYARIQEIVWDDAPWIFLASDQVISGYKSYCSGIQLYPDGAI
                     DCSKAKLN"
BASE COUNT          539 a          454 c          442 g          618 t
ORIGIN      
        1 ccggggatca aatgaataaa cataaagatc aaaaaagaaa ttaccaaaag caacggaatg
       61 gttcccaata ctcttttaac aaaatagcgg cccaaagaat acctccgatt tgatcgttat
      121 gtttcgccag ttaaaacttt tcagatcaac gaaactttca cttttaataa taacttacgg
      181 ttgaagaaaa accctgcatt catttggatc gctcgtgaaa atccggagtt cggatttaga
      241 ttgtgatcga tccgcaactg atatgcaggg tttttgaaga tcattttgcc ggttacctga
      301 tttcaatcaa ccgagatgac cggttgattg gattctcgat taattcaatt ttgcttttga
      361 acaatcgatg gctccgtccg gatacagttg gatgccgctg caataggatt tgtaaccgct
      421 gatcacctga tcactcgcca agaagatcca cggcgcgtca tcccagacga tttcctgaat
      481 tcttgcgtac ttttcctgaa gaatcttcgg atcggttatt gccaaggctt catccatcag
      541 tttgttgact tcttcattgt tatagtacgc tgagttatat tgacccggcg ggatgctctc
      601 tccgtgcaga attgcgcgaa tcgcaccgtc cgcatcgaat gaagaagccg accaatttac
      661 ataccacata ttcacttcgg ctttatctct ttcggcttgg attttttctg cgacgacatt
      721 ggcttccatc ggcattactt cgacatcgat tccgatttgt ttcaattgtt gttgaataaa
      781 ttgcatccct ttttcttcgg aagtaatgtt atctccccaa attgtaagtt tgaatccgtt
      841 ttccgctccg gcttctttca tcagtgcctt ggctttttca agattaaagt catagggagg
      901 ctgaggagca tagtattgga tcgtctcggg atagcaggaa tagactttgg aagcataacc
      961 gttgaaaacg gttttgatat atgcgtcttt gtcgacggca taattgatag cctgccgaac
     1021 gcgcttatct tttaattgtt ccaactcggt attgagcgta acataccgca tgatattgga
     1081 ggtctttgca tcgatcagaa tatctttttt cccttcgacg accttaatct gagtggtcgg
     1141 catgggataa atgtaatccg cttcaccggt ttgcagcatt gcgactcggg cgccgtcttc
     1201 cgggactgct ttgaaggtca acgatgcaac ctgtggggct ccgtcccaat atttttcaaa
     1261 aggaacgaca gtaacatgat cgccttccac acgatctttg aatgtgtaag ggcctgtccc
     1321 gcagggttcc cgcaggattt tctcttcgcc tgcggcaatc gtcgcgggtg cgatgaaatc
     1381 gaacatggtc attttattca ggaaggtgtt gttcggtttt gcaagtgtca ggataacggt
     1441 ataaggatcg ggatattcgg cattcgcaat ctgcttcaca atccgattct gtcgcaaact
     1501 ttcatcctta cagcgatcga tgttggcttt caccgcatcc gaattgaatt cggtgccatc
     1561 atggaaaagt acgccggagc gaaggtggaa cgtataagtc atcccatcat ccgaaatttc
     1621 atagctttca gccagcagcg gtacgatttc ctgtttctcg ttgaacttta cgagtgattc
     1681 gtacatggtc cgtgtcgcag agatggtcag tgtgtctgag acattgtgcg gatcgagcgc
     1741 ccggatatcg ttattgaaag caataaccaa atcctgatcc ggggctttcg cggcattgtc
     1801 ggattgtgcc gataccgtta acaatccctg agcggcaaaa gcaaaaacgg tcagaaaaat
     1861 aaaaacgaat aagatttttc ttttgtacat ataaatctcc tttcaaaatt ttcgcaggaa
     1921 aggaaatctc tcctgcatat acactatgga atcgaacgat tcaaatctcg cttatcgaca
     1981 aagtcagacg aatattttat gctttattat taacattata tgcaataata acatggccac
     2041 tcaagtaatt aat
//
LOCUS       DAOMIV010000398         2051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_62089_length_2051_cov_4.494990, whole genome shotgun sequence.
ACCESSION   DAOMIV010000398 DAOMIV010000000
VERSION     DAOMIV010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62089_length_2051_cov_4.494990"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PKV59_08405"
     CDS             <1..296
                     /locus_tag="PKV59_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46807.1"
                     /translation="AKGNIEFVTIFKHALRNALIPVITLVGYQIGYLFGGVVIIEQVF
                     ALPGLGRFLLNGISQRDYALVQGTILFIAANFVLVNLIADVAYGFADPRIRYD"
     gene            299..1195
                     /locus_tag="PKV59_08410"
     CDS             299..1195
                     /locus_tag="PKV59_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46808.1"
                     /translation="MNRELISEEAKSQQRKRNFQYSAVGRLFRNPSGVIGLILVIIFI
                     VIAVFSDYLMPYDPIKNYPKERLQAPSSEHLAGTDEMGRDCMSRLMKGATNSLSIAFV
                     SVAFAGIIGTFLGSLCGYYGGWFDSLIMRLMDLIFSFPAMLLALAISAALGAGFLNTV
                     MAISIVYLPIFSRTARGSVLQIREMEYIEAARCVGAKNMRIIFRHILPNALAPLIVQV
                     SLGLSWAILTESSLSYLGLGTQPPNPSWGSMLSQSRVMMELAPWLMIGPGAAIMLAVL
                     GFNLLGDAIRDLTDPRLRSRGL"
     gene            1203..1670
                     /locus_tag="PKV59_08415"
     CDS             1203..1670
                     /locus_tag="PKV59_08415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQP46809.1"
                     /translation="MSSVSVSRIREEEIPACAQIMAGNSLWQHYDVTYESALARFQNG
                     YAEQATIFIAHEGEEVCGFCWVAEKGAFNRSAYIMLIGVDPLRQSRGIGKILLEETEK
                     YLATKSVDVFLTVSDFNIGAQKFYSGMGYKTVGEIPDYVKPGITEIIMRKVLR"
     gene            1708..>2051
                     /locus_tag="PKV59_08420"
     CDS             1708..>2051
                     /locus_tag="PKV59_08420"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HQP46810.1"
                     /translation="MIILGMMSGTSVDGIDAVLVDIVGAPPRFEWKIIGHHQMPFPEA
                     IRREIFDCFDPKKGTVDRICSLNFKLGRVYGEAALETIRQLGYSPEQVRFIGCHGQTV
                     WHIPANEPDAST"
BASE COUNT          514 a          402 c          534 g          601 t
ORIGIN      
        1 gagcgaaggg aaatatagag ttcgtgacca tctttaagca tgcgctccga aatgcgctta
       61 tcccggtgat taccttagtc ggctatcaga tcggatatct tttcggcgga gtagtgatta
      121 tcgaacaagt atttgcgctg ccggggttgg gaagatttct gttgaacgga atttcccagc
      181 gtgattatgc tctggtacaa ggaacgattc tgtttatcgc cgcaaatttt gttctggtga
      241 atctgattgc ggacgtcgcc tatggctttg ccgatccgcg gataaggtat gactgaaaat
      301 gaatcgggaa cttatctctg aagaagcgaa gtctcaacaa agaaaaagga attttcaata
      361 ttccgctgtg ggccgtcttt ttcggaatcc aagcggagtt atcggattga tcttagtgat
      421 tattttcatt gtgatcgccg ttttctcgga ttatttgatg ccgtatgacc cgataaaaaa
      481 ttatccgaaa gagcggttac aagcgccctc ttccgaacac ttggccggta cagatgaaat
      541 gggccgagat tgtatgagtc gactgatgaa aggcgctacg aattctttat cgatcgcttt
      601 tgtctctgtc gccttcgcgg gaattatcgg cacttttttg gggtcgcttt gcggatatta
      661 tggcggctgg tttgacagct taatcatgcg cctgatggat ttaatttttt catttccggc
      721 gatgctgttg gcattagcca tttccgctgc gttgggagcg ggctttttga ataccgtgat
      781 ggcgatttcc attgtctatc tgccgatttt ctctcgaacc gcgcgcggtt cggttttgca
      841 gattcgtgaa atggaatata ttgaagctgc ccgatgtgtc ggtgcaaaaa atatgcggat
      901 tatttttcgg catattttac cgaatgcgtt agctcctttg attgttcagg tcagcctggg
      961 attatcttgg gcaatcttaa ccgaatcttc tctgagctat ttgggattgg gaacgcaacc
     1021 ccccaacccg tcctggggtt ccatgttgag tcaatcaaga gttatgatgg aattggctcc
     1081 atggttgatg atcggtcccg gtgcggctat tatgctggct gtattggggt ttaatttgct
     1141 cggtgatgcc attcgtgatc tgacggatcc gcgcctgcgt tcaagggggt tatgaaataa
     1201 agatgtcttc tgtcagcgtt tctcgaattc gagaggaaga aataccggct tgtgcacaaa
     1261 ttatggccgg aaattcgctt tggcaacact atgatgtgac ttatgaaagt gccttggcaa
     1321 gatttcaaaa cggatatgcg gaacaggcga cgatttttat tgctcatgaa ggcgaagaag
     1381 tatgtggttt ttgctgggta gcggagaaag gcgctttcaa tcgttcggct tatattatgt
     1441 tgattggggt tgatcccctg agacaaagcc ggggcatagg aaaaatactt ctcgaagaaa
     1501 cagaaaaata ccttgcaaca aagagtgtcg atgttttctt aaccgtttcg gattttaata
     1561 taggcgcaca aaagttttat tcaggcatgg ggtataaaac ggtgggagaa atcccggatt
     1621 atgtcaagcc cggaatcacc gaaataataa tgcggaaagt tttacgataa atatcaacct
     1681 tctgtgatgg aagtaaagga aaagaaaatg attattctcg gaatgatgtc cggaacctcc
     1741 gtagacggaa tcgatgcagt actggtcgat atagtaggcg cacctcctcg tttcgaatgg
     1801 aaaattatcg gtcatcatca gatgcctttc ccagaagcga tccgcaggga aatctttgat
     1861 tgttttgatc cgaaaaaagg gacagttgat cgaatctgca gcctaaattt taaattggga
     1921 cgtgtttacg gcgaagcggc gttggaaact atccggcaat tggggtattc accggaacaa
     1981 gttcgattca tcggctgtca cggtcagacg gtatggcaca taccggcgaa cgaaccggat
     2041 gcatccactt t
//
LOCUS       DAOMIV010000399         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_62256_length_2048_cov_2.693427, whole genome shotgun sequence.
ACCESSION   DAOMIV010000399 DAOMIV010000000
VERSION     DAOMIV010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62256_length_2048_cov_2.693427"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PKV59_08425"
     CDS             <1..680
                     /locus_tag="PKV59_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46811.1"
                     /translation="FSRISTLSFQMSLFFEGYLKKICEGYQNIYSVYSGGDDLFLIGP
                     WQEITGLAPAIRQKFNQYTCENPDFHISGGMAFIHGKYPVIQAAKDAGDAQEKAKSKE
                     KKNAFTFLDEAWYWEDYLKLQGYEDQLRIIIEQKDCPASLLQLLQQLDREKIESGKPV
                     YGRWLWMGDYQFVRMKERYAQNLELVIALDTLHRQISQADYRDIHYWAKAARWAQLAL
                     RTDQKGE"
     gene            683..1138
                     /gene="csm2"
                     /locus_tag="PKV59_08430"
     CDS             683..1138
                     /gene="csm2"
                     /locus_tag="PKV59_08430"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HQP46812.1"
                     /translation="MIQNNQSYRGGKNQPTETKFTTQDKESIRKIITEDDDGKELVEF
                     SKRMGEQFVKDGLTKSQFRNIFSEARRIEAYWGKDPVTAERRLNLLIPKLAYQVGKDP
                     RPYQIKYFADVITEAITQVNRSSDKEKSFEKFMDLFEAMLAYHRSFGGK"
     gene            1156..1941
                     /gene="csm3"
                     /locus_tag="PKV59_08435"
     CDS             1156..1941
                     /gene="csm3"
                     /locus_tag="PKV59_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HQP46813.1"
                     /translation="MSKIKLAGRVFIRCEIEAVTGLHIGGTGGSLEIGGIDNPVIRDP
                     ITLEPLIPGSSLRGKMRSQLEKKLGSLQNQKVGKASIHVCKGEDAKNPCPVCRLFGLP
                     ATEGIGTPAILLVRDVRLTSDSIKMLNNAHTDQMFTETKAEVAIDRVTAAATPRSIER
                     VPAGSVFGPAEMVLSFYRSEDLSSLSDLLDCMQLAEDDYLGGGGSRGSGKIHFRELTL
                     SLRTAEDYSTETKLGKYADLKQFKDEYPQLLASIKKDLIFAEE"
BASE COUNT          597 a          429 c          525 g          497 t
ORIGIN      
        1 cattctcccg aatttcgaca ttaagctttc agatgtctct cttctttgaa ggatatttga
       61 aaaaaatctg tgaaggatat cagaatattt acagtgtcta ttccggcgga gatgatttgt
      121 ttttgatcgg tccttggcag gaaattacgg gcttggctcc tgccatcaga caaaaattca
      181 atcaatacac ctgtgaaaac cccgattttc atatcagcgg aggaatggcg tttatccacg
      241 gaaaatatcc ggtgattcaa gccgcaaaag atgccggcga tgcgcaggaa aaagcaaaat
      301 cgaaagagaa aaaaaatgcc ttcacatttt tggacgaagc ttggtattgg gaggattatt
      361 tgaaattaca gggatatgaa gatcaattga gaataattat cgaacaaaaa gattgtccgg
      421 cttccctctt gcagttattg cagcaattgg atcgggaaaa aatcgaatcg ggaaaaccgg
      481 tctacggccg ctggctctgg atgggtgatt atcaatttgt tcgaatgaag gaaagatatg
      541 cacaaaattt ggagctggta atagcgttgg acacgctaca ccggcaaatc agccaagccg
      601 attatcggga tattcattat tgggcaaaag ctgcccgttg ggctcaattg gcgttgcgaa
      661 cagatcagaa aggagaatga atatgataca aaataaccaa agctacagag gtggcaaaaa
      721 tcaaccaacc gaaacaaagt ttacaactca ggacaaagaa tccatccgga agatcatcac
      781 ggaagacgac gacggcaaag agctggttga attttccaag cggatgggcg aacaatttgt
      841 caaagatgga ttaaccaaaa gtcagtttcg gaacattttt tccgaagctc gccgtattga
      901 agcgtattgg ggaaaagatc ccgtcaccgc ggaacggcgt ctaaatctgt tgataccgaa
      961 actggcttat caggtgggaa aggaccctcg tccctaccaa atcaagtatt ttgcggatgt
     1021 gatcacggaa gcgattacac aggtcaaccg atcgtctgac aaagaaaagt catttgaaaa
     1081 attcatggat ctgttcgaag ccatgttagc ttatcatcgc agcttcggcg gcaaataacg
     1141 aaaggggaag aaatgatgtc gaaaataaaa ttagccggaa gagtttttat tcgttgcgaa
     1201 attgaagccg ttaccggtct tcatattggt gggacgggcg gatcgcttga aataggcggg
     1261 atcgacaacc cggtcattcg ggatccgata accttggaac ctttgatccc cggttcatcg
     1321 ctgcgcggca aaatgcgatc gcaattggag aaaaaattgg gcagtctgca gaaccaaaaa
     1381 gtcgggaaag caagtatcca tgtctgtaaa ggcgaggatg cgaaaaatcc ctgtccggta
     1441 tgccgtctct tcggtttacc ggcaacggaa gggatcggta caccggccat tctactggtg
     1501 cgggatgtgc gtttgacgag tgattccatt aaaatgttga ataacgccca taccgatcag
     1561 atgtttaccg aaaccaaagc cgaagtggcg atcgaccggg tgacggcagc ggctactcct
     1621 cgcagtatcg aacgcgttcc cgccggatcc gttttcggtc cggctgaaat ggtgctttcc
     1681 ttctatcgat cggaagatct atcctctctg tccgatttgc tggattgtat gcagttggcg
     1741 gaagatgact accttggggg cggcggttcc cgtggcagcg gaaaaattca tttccgcgag
     1801 ctgactcttt cgctgcggac cgcggaagat tacagtacgg agacgaagtt gggaaaatat
     1861 gccgatctga aacagtttaa ggatgaatac ccgcagctcc ttgcttcgat taaaaaggat
     1921 ctgatattcg cagaggagtg aatcatgaat ttggaaatct accatattcg gggttcggcg
     1981 tttcacttcg gcagacaggg ccccggggag gttgaaagca acgtgacttt tccttccgac
     2041 agcctttt
//
LOCUS       DAOMIV010000400         2046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_62333_length_2046_cov_3.193370, whole genome shotgun sequence.
ACCESSION   DAOMIV010000400 DAOMIV010000000
VERSION     DAOMIV010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62333_length_2046_cov_3.193370"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="PKV59_08440"
     CDS             complement(<1..801)
                     /locus_tag="PKV59_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQP46814.1"
                     /translation="MTFDNFIQFVVMGLSLGAMYSLISIGYTIIYGILKLINLAHGDF
                     FMLAGFLGMWGALYYGVPLWLSLVFGLALTVGVILFVNWFIYRPLRQYKMAAFTSTFA
                     ISMIVQASVIMFLSARAQAFPHPDFLNTPIKIGNIIIPMVTPFIIVIALILFGILTLI
                     VNKTKIGRAMRAVSLDMEMVTLMGVNVENVIAFSFALSVVYAGMGAYLWGMRYPAFDP
                     NIGVVTGLKGFIGAVIGGIGSIPGAMLGGFLLGFAEIMLIGFFPEYTNF"
     gene            complement(993..>2046)
                     /locus_tag="PKV59_08445"
     CDS             complement(993..>2046)
                     /locus_tag="PKV59_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46815.1"
                     /translation="GIVDSMTGGHAAYGLASLDGYKLANKYRDSALGKKIEFIIVDTK
                     SDKAETALATSRAIADGAVALLGTASSGFTIAMNEVAAEKGIPTVTNFSTNPLVTQGN
                     DIAFRSCFIDPYQGWALAKFAIENLGAKTSVLLVDISTDYPVGVANYFRETWNEMTGD
                     SKTLLGYYSIMLGDRDFTAQLTAIKQLNPDVVVVPDDYAEVGLILKQARELGMTMPFL
                     GGDAVDLPEIEGIAGDAIKNDFYFSTHWHPDAFQNEASKAFGDYYRKEYNREPDAAAA
                     TAWDAYNMLIDAIERAGSTDPTAIKEALYKTDMEGVTGHIKIDENGDAIKPVTILGYK
                     DGVKKIIAIIQPEEKK"
BASE COUNT          644 a          485 c          385 g          532 t
ORIGIN      
        1 aaaattggta tactcaggga agaatccgat cagcataatt tctgcaaatc ccagcaaaaa
       61 tccaccgagc atcgcaccgg gaatacttcc gattcctcca atgacggctc cgataaaccc
      121 cttgagaccg gtgacgacac cgatattcgg atcgaaggcc ggatagcgca ttccccaaag
      181 ataagcaccc attccggcat aaacaacgct caaggcaaat gaaaaggcaa tcacattttc
      241 gacattaacg cccatcagcg taaccatttc catatccaga ctgacagctc tcatagcccg
      301 cccgattttg gttttattaa caattaaagt aaggattccg aataaaatga gagcaatcac
      361 aataataaac ggtgttacca tcggaatgat aatattgccg attttgattg gtgtgtttaa
      421 gaaatcagga tgcggaaacg cttgagcacg tgcggaaaga aacatgataa ccgatgcttg
      481 cacaatcatc gaaattgcaa aagtacttgt aaaggcagcc atcttatact gtcgcaaagg
      541 gcgataaata aaccaattga caaaaagaat tacaccgacc gtcagtgcca gaccgaaaac
      601 aagagagagc cacaacggaa caccgtaata tagcgctccc cacattccca gaaaacctgc
      661 caacataaaa aaatcaccgt gtgcgagatt gattaatttt aaaataccat aaattatggt
      721 atatccgatt gaaattaaag aatacatcgc tcccagtgat agccccatga ctacgaactg
      781 gataaaatta tcaaatgtca tgttaaaaat accttcttcc gggttcagga taaataactc
      841 aaaaaaacgg ataaacgaaa aatgagttac cttctgcaaa tattcaagaa tgacaacaaa
      901 actatttatt aaaaaatata aaaagaaaac gttcaaaaaa ctatttgggg catataaaat
      961 cgccccaaat agttttcaaa tgagaattta ttttattttt tctcttccgg ttgaatgatt
     1021 gcaattatct tcttgacacc atctttatat ccgagaatcg ttaccggttt gatcgcgtcg
     1081 ccattttcat caatcttgat atgaccggtc accccttcca tatccgtctt gtacaacgct
     1141 tctttaatgg cagtcgggtc ggtagatccg gcacgttcga tcgcatcaat caacatattg
     1201 tatgcatccc aggctgtcgc agccgcggcg tctggttctc gattatattc tttccggtaa
     1261 taatcgccaa aagcctttga tgcttcgttt tgaaatgcat ccggatgcca atgagttgaa
     1321 aaataaaaat catttttaat agcgtcgcct gcaattccct cgatttcggg aaggtcaacc
     1381 gcatctccgc ctaaaaatgg cattgtcatc cccaattccc gtgcctgttt gagaatcaaa
     1441 ccgacttcgg cataatcgtc tggaactacg actacatccg gatttaattg tttgatagcg
     1501 gtcaactgtg cggtgaaatc acggtcaccc aacataatcg aataatatcc taagagggtt
     1561 ttggaatcgc cggtcatttc attccacgtt tcgcggaaat agtttgccac ccccaccgga
     1621 tagtcagtcg aaatatcgac cagtaataca gaagtcttcg ctccgaggtt ttcaatggca
     1681 aatttggcta aagcccaccc ctggtaagga tcaataaagc aagaacggaa agcgatgtca
     1741 tttccctgag tcaccaaagg atttgttgaa aagttggtta ctgttggaat tcccttttca
     1801 gcagccactt cattcatcgc aatcgtaaaa ccactggaag ccgttcctaa caaagccacg
     1861 gcgccatctg cgatggctct ggaagtcgcc aaagctgttt ccgctttatc gctttttgta
     1921 tccacgatga tgaattcaat cttctttcca agcgctgagt cgcgatattt attcgccaat
     1981 ttataaccat ccaacgatgc gaggccgtaa gcggcatggc cgcccgtcat actatcaacg
     2041 attcca
//
LOCUS       DAOMIV010000401         2045 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_62373_length_2045_cov_3.711055, whole genome shotgun sequence.
ACCESSION   DAOMIV010000401 DAOMIV010000000
VERSION     DAOMIV010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62373_length_2045_cov_3.711055"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1022)
                     /locus_tag="PKV59_08450"
     CDS             complement(30..1022)
                     /locus_tag="PKV59_08450"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HQP46816.1"
                     /translation="MNQDKNNVLTEIRKIIENASNIALFCHIRPDGDAIGSMLGLGWS
                     LEEMGKKVQYVCPDELPVQGWLNPQTIGKNTRFVKAPEPYDCSVLLDVSTLSRAGYFF
                     EQPDIGQPDICIDHHVSNSGDAKINLIDPSAPATAIIVAHLIEELNFPMNKEIAEALL
                     SGIIMDTQGFSTSNTNPDSLRVTAKLMELGADLYWGIQNILKAHSLEAAKYWHCGLSK
                     LETRGKILWTVLTDTDRVHSGYTGRDDAGLIDYLASTEGFLATVLFNEQNDGRVKISW
                     RSRPGVNVAAIAEEFGGGGHRQAAGADVNGPIDDVVERVLACTEKGVRSAEQSS"
     gene            complement(1148..1534)
                     /gene="rbfA"
                     /locus_tag="PKV59_08455"
     CDS             complement(1148..1534)
                     /gene="rbfA"
                     /locus_tag="PKV59_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQP46817.1"
                     /translation="MPSPIRLKKIAERIRDDLSEMLLYEIDDPRLQGVMITDVNVDRE
                     LYYANIYVSALEGKERSAEILAGLENASGYMRHALSQSIELRTFPKLRFFWDPTPEKA
                     DRVETLLRQLKEEQEAKNKNEQKGIA"
     gene            complement(1556..>2045)
                     /locus_tag="PKV59_08460"
     CDS             complement(1556..>2045)
                     /locus_tag="PKV59_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQP46818.1"
                     /translation="GNISENDVMLASASHGVVIGFNVQADPVAKKKAENEGVSIRIYD
                     IIYRLIEDIEKALKGMLEPEFKETVIGRAEVLTTFSISKVGTIAGCRVKEGDIRRNCK
                     VHVKRGNEIIFTGEISSLKHEKEDVKEVKTGLDCGIGLKGFEDFKPGDILEAFVLERF
                     GM"
BASE COUNT          522 a          533 c          378 g          612 t
ORIGIN      
        1 taattaaaac cggacactcg ggaaggaaat tacgatgatt gttcagccga tcgaacacct
       61 ttttcggtgc aggccagcac ccgttcaacg acatcgtcga tcggtccgtt tacatcggct
      121 ccggccgctt gccggtgtcc gccgccgcca aactcttccg caattgctgc cacatttaca
      181 ccgggtctgc tccgccatga tatttttact cgtccgtcgt tttgttcatt aaataaaacc
      241 gtagcaagaa aaccttccgt cgaagccaga taatcgatta aaccggcatc gtctctgccg
      301 gtataccctg agtgcacccg atcggtatct gtcagcacgg tccataaaat tttccctcga
      361 gtttccaatt ttgaaagccc gcaatgccaa tattttgccg cttccaaaga atgagctttc
      421 aaaatatttt ggatccccca atacaaatcg gcgcccagtt ccatcaattt cgccgtgaca
      481 cgcagcgaat ccggattcgt attggatgtg ctgaatcctt gcgtatccat aataatgccc
      541 gataaaagcg cttccgcaat ctctttattc atcggaaaat tcaattcttc gatcagatga
      601 gcgacgataa tcgcggtagc cggagcagaa ggatcgatca aatttatctt agcatctccg
      661 gaattggaaa catgatgatc gatacagata tcgggttgcc cgatatcggg ctgttcaaaa
      721 aaatatcccg ctcgagaaag cgtgctgaca tccaagagta cggaacaatc atacggctcc
      781 ggagctttta caaaacgggt gttttttccg atggtttgag gattcaacca accttggacc
      841 ggcagttcat ccggacagac atattggact tttttcccca tctcttccaa cgaccagcct
      901 aatccgagca ttgagccgat ggcatcgcca tccggacgaa tatggcaaaa taaagcaata
      961 ttcgacgcgt tttcaatgat tttgcggatt tccgtcagta cattgttctt gtcttgattc
     1021 atgagctctc aaattacgat attttttgat aaattccccg tccgattcta aaaccatttt
     1081 aatcattttt taatcgttct atttggttcc gattcggaag aattcgataa ataagggatt
     1141 gcctttttca ggcaatccct ttttgttcat ttttattttt tgcttcctgt tcttctttga
     1201 gctgtcttaa taaggtttcc acccgatccg ctttttccgg agtaggatcc caaaaaaagc
     1261 gcaatttcgg aaaagtcctc aactcgatcg actgcgaaag agcatgcctc atatatcctg
     1321 acgcattttc caatcccgcc aaaatttcgg cgctgcgttc cttcccttcc aaagcggaaa
     1381 cataaatatt ggcataataa agttcccgat ccacgttgac atccgttatc atgactccct
     1441 gtagtctcgg atcatcaatt tcatacagca acatttcgga taaatcatcc cgaattcgct
     1501 ctgctatttt tttcaatcgt atgggcgatg gcatgataat tcctcctctc ttacatcaca
     1561 taccgaatct ttccaaaacg aaagcttcga gtatatcccc cggtttaaaa tcttcaaatc
     1621 cttttaatcc gataccgcaa tccaatccgg ttttcacttc ctttacatcc tccttttcat
     1681 gcttcagcga actgatctca cccgtaaaaa tgatctcatt tccgcgtttt acatgaactt
     1741 tgcagttccg tcggatatcc ccttctttca ctctgcatcc ggctatggtt cccacttttg
     1801 aaatggagaa cgtcgtcaaa acctccgctc gcccgataac cgtttcttta aattcgggct
     1861 ccaacattcc tttcagagct ttttcgatgt cttcgatcaa acggtaaata atatcgtaaa
     1921 ttcgaatcga gacaccttcg ttttcggctt ttttcttggc aaccgggtcg gcttggacat
     1981 taaatccgat gacaactccg tgagacgcgg atgcaagcat gacatcattt tcactgatat
     2041 tgccg
//
LOCUS       DAOMIV010000402         2042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_62517_length_2042_cov_4.500252, whole genome shotgun sequence.
ACCESSION   DAOMIV010000402 DAOMIV010000000
VERSION     DAOMIV010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62517_length_2042_cov_4.500252"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PKV59_08465"
     CDS             <1..748
                     /locus_tag="PKV59_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46819.1"
                     /translation="REAIRDYADMGYDLVIAHGSQYGTSLQEIAPDYPETAFAWGNGT
                     DYYGQPNISVYQARAEQGGYVFGQIAAKYTNKGKLGVCGPVESGDAKTYIDGFKYGAE
                     QGGSQVSVTYTSSFSDVSLMAAAAETHMAEGATLLTGTSQSIPGAVSVVKEKGGVWMG
                     VQWDTIPLAPKNVLVSLVYDWTPILNDIIANMKEGVYGGKAYDLTFEDGGLKFVWNTE
                     NMEIDPELIKMADELAAKITAGEINPLPAE"
     gene            878..>2042
                     /locus_tag="PKV59_08470"
     CDS             878..>2042
                     /locus_tag="PKV59_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46820.1"
                     /translation="MTEREGSNQRTNHIKKLQMVDITKQFPGVLANDKVNLEVNEGEI
                     LALLGENGAGKSTLMKQLYGLYKPDSGKILINGEEKVFNSPVDAINAGIGMIHQHFML
                     VSNLTVVENVALGLKSSRGLFLDLDVVEKRILEICETYKLKVDPKAYIWQLAVGEQQR
                     VEIIKALYRGADLLILDEPTAVLTPQEVEEFFVILRKMAEAKHSLIFISHKLNEVIEI
                     SDRIVILRDGRSVAEKKNENITKTELAEKMVGRPVVLQYEKTPLNQRDIRLKIVNLHV
                     ENDWGNEKVCGVDLEIHGGEILGLAGVSGNGQPELAQALAGLRKPTQGKIYLNGKDVT
                     DATPYERTLAGQSYIPEERNKDGVIKDFNISENFILEDHDKEPYSKHTFFNFAN"
BASE COUNT          602 a          383 c          546 g          511 t
ORIGIN      
        1 acgggaagcg attcgggatt atgcggatat gggatatgac cttgtgattg ctcacggttc
       61 ccaatacggg acttccttac aggaaatcgc tcccgattat ccggagaccg catttgcttg
      121 gggaaatggc acagattatt acgggcagcc gaatatttcg gtttatcagg cacgggctga
      181 acagggcgga tatgttttcg ggcaaatcgc ggcgaaatat accaataaag ggaaattggg
      241 tgtttgcgga ccggtcgaat ccggtgatgc caaaacttac attgatggtt tcaagtacgg
      301 tgccgaacag ggtgggtcgc aggtcagcgt gacttatact tcgtcatttt cggatgtttc
      361 tttgatggcg gcagccgcgg aaactcatat ggcggaaggc gcgacacttt tgaccggaac
      421 ttctcaatcc atccccggag ccgtcagcgt ggtaaaggaa aagggcggtg tatggatggg
      481 cgtccaatgg gatacgattc ccctcgctcc gaagaatgtt ttggtcagct tggtatatga
      541 ttggactccg atcctgaacg atatcattgc caatatgaag gaaggagtct acggcggaaa
      601 ggcttatgat ttgacctttg aggacggcgg tctgaaattt gtctggaata cggagaatat
      661 ggaaatcgat ccggagctga ttaaaatggc ggatgagctg gctgcgaaaa taacagccgg
      721 agaaattaat ccgctgccgg cggaatagag gagccgtatc gatcgtttca ttcatcgtaa
      781 aaaatcggag ggagcttgcc actccctccg aaagatgatt tagagtttga tttgaaaaaa
      841 agcaacggaa cggaaatgtt gccggaagga atttgttatg accgagaggg aaggatctaa
      901 ccaacgaaca aaccatatta aaaagctgca gatggtggat attaccaaac agtttccagg
      961 tgtcctcgca aatgataaag tcaatctgga agtaaacgaa ggagagattt tggctttatt
     1021 aggtgaaaac ggcgccggaa aaagcacctt aatgaagcag ctttatggct tgtataaacc
     1081 cgacagcgga aagattctaa taaacggaga agagaaagtt tttaattctc ctgtcgacgc
     1141 gataaatgcg gggatcggga tgatccatca acattttatg ttggtatcca acctgaccgt
     1201 tgttgagaat gtggcgttag gtctgaaatc cagtcggggc cttttcttag atttggatgt
     1261 agttgaaaaa cggatactgg aaatttgcga aacgtataag ctaaaagtcg atccgaaagc
     1321 ctatatctgg caattggcgg tcggcgaaca acagcgtgtg gaaatcatta aagcgcttta
     1381 tcgcggtgcg gatcttttga ttttggatga gcccaccgcg gtattgactc cgcaagaggt
     1441 ggaagagttt tttgtcattt tacggaaaat ggcggaagca aaacattctt tgatatttat
     1501 ctcgcacaag ctgaatgaag tgattgaaat cagcgatcga attgttattc tcagagatgg
     1561 tcgaagcgtt gcggaaaaaa agaatgagaa tattacaaag accgagctgg ctgaaaaaat
     1621 ggtcggtcgc ccggtcgttc tccaatatga gaagacgccg ttaaatcaaa gggatatccg
     1681 attaaaaatt gtcaatttgc atgtcgagaa tgattggggg aatgaaaagg tttgcggtgt
     1741 tgatcttgaa atccacggag gtgaaattct cggattggcc ggtgtttccg gcaacggaca
     1801 acccgaactt gctcaggcat tagccggcct acgcaaaccg actcagggga aaatctacct
     1861 gaacggaaaa gatgtgaccg atgcgacacc ttatgaacga acactggccg ggcaatctta
     1921 tattcccgaa gagagaaata aagatggtgt gatcaaggat tttaatatat ccgaaaattt
     1981 cattttagag gatcacgata aagaacctta ttcaaaacac acttttttca attttgcgaa
     2041 ta
//
LOCUS       DAOMIV010000403         2039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_62707_length_2039_cov_2.733367, whole genome shotgun sequence.
ACCESSION   DAOMIV010000403 DAOMIV010000000
VERSION     DAOMIV010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62707_length_2039_cov_2.733367"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKV59_08475"
     CDS             complement(<1..643)
                     /locus_tag="PKV59_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HQP46821.1"
                     /translation="MYQYPKNLYTDVRIETVSKTNIQFENGELKENKTVSEKGAMVRI
                     YDGKRWYYSATTDLNSIQNEIDDLAEMAVPNSAILQDSVVDKLEINRGMDLRYQQDDV
                     SLISNDQKMALVKSYLELISDYPEIRNSKIFYLDNHTVKHFVSGKGADITFDTQNCTI
                     VVRYTIMANDFPQDGQANIYKIRFHELAGHQREVRDEIEKDLDYAKRAVPVKPG"
     gene            complement(750..1514)
                     /locus_tag="PKV59_08480"
     CDS             complement(750..1514)
                     /locus_tag="PKV59_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQP46822.1"
                     /translation="MKDSYTIGEFSKMFGLNVQTLRYYDSIGIFRPAYRDKWSGRRKY
                     AFDQVYQLASIRYLRRIGYSLEDIAEYLDNLQSDTSLDVLKKRSDEIHRELDELLDID
                     HAIQRKIRFIEHELSEIREISEIKICEYGPRPYIPLGQEEVLYHHDSFYFYPTIAFYK
                     EDLKYFGAYLYQDNSQFPEDFKVNKEKVEYIEAGKYLCAYHLGPYEEIYQTQKKLRNA
                     YSNLKLSDLMVNFNIIDQFVEKDRSKYITSMQIRIL"
     gene            complement(1557..>2039)
                     /locus_tag="PKV59_08485"
     CDS             complement(1557..>2039)
                     /locus_tag="PKV59_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46823.1"
                     /translation="VYLMYLLVLIFLFIYKKTEWGVIFTAVGEHPSAADTAGINVFRI
                     RYLACMVNGLLGGLAGSYFTLAQLGFFMEDITAGKGYIALVTVILGRRNPLGILCSAL
                     LIGLADAVQFQLQTMNIPIPSQAFSILPYIAAVFVLLFSAGKSSDPSALGIPYERDNR
                     "
BASE COUNT          614 a          451 c          363 g          611 t
ORIGIN      
        1 cacccggttt aaccggaaca gcccttttgg cataatcaag atccttttca atctcatctc
       61 ggacctcacg ctgatgcccg gccaattcat gaaaacggat cttatagata ttcgcctgcc
      121 catcttgcgg aaagtcgttc gccatgattg tgtatcggac gacaatcgtg caattttgag
      181 tatcaaatgt gatatcggcg cccttaccgg aaacaaaatg tttcacagta tggttatcca
      241 gataaaagat ttttgaattt cgaatttcgg gataatccga gatcaattca agataacttt
      301 ttaccaatgc cattttttgg tcgttggaaa tcaaggaaac gtcatcctgc tgatagcgca
      361 aatccattcc ccgatttatt tcaagcttat caaccaccga atcttgaagg atcgccgaat
      421 tcgggacagc catttccgcc agatcatcta tttcattttg aatcgaattc aagtcggttg
      481 ttgcgctgta ataccagcgt ttcccatcat aaatccggac cattgcccct ttttcggata
      541 cggttttatt ctctttcagc tcaccgtttt caaattgaat gttggttttt gaaactgttt
      601 cgattcgaac atcggtatat aaattttttg gatattgata catagagcat tctccttacg
      661 tcaattatag cgagtttgaa tacaaactgt tatcggcaaa tcagccggac gcggttacca
      721 agtgaaatta ctaaaatgat ttttttgcgt cacaaaatac gaatttgcat gctcgttata
      781 tactttgatc ggtccttttc gacgaactga tcgataatat tgaaattaac catcagatcc
      841 gaaagtttta aattcgagta agcgttgcgc aatttttttt gagtctgata aatttcttca
      901 tacggtccca aatgataagc gcataaatat ttgcccgctt cgatatattc aactttttct
      961 ttattgactt taaaatcttc ggggaattga ctgttgtctt gatataaata agcgccaaaa
     1021 tatttcagat cttctttata aaatgcaatg gtcggataaa aataaaaaga atcgtggtga
     1081 tacaatactt cttcctgccc taaaggaata taaggtcggg gtccatattc acatatcttt
     1141 atttcagaaa tttctcgaat ttcgctgagt tcgtgttcaa taaaacgaat tttgcgctga
     1201 atcgcatggt caatgtcaag taattcatcc agttcgcgat ggatctcatc cgatcttttt
     1261 ttcaaaacat ctaatgaagt gtccgattgc agattatcca aatattcggc aatatcctct
     1321 aaagaatagc cgatccggcg taagtagcga atcgaagcca attgatagac ttgatcaaac
     1381 gcatattttc gccgacccga ccatttatcc ctatatgccg gtctgaaaat tcctatcgaa
     1441 tcataataac gaagtgtttg gacattcaat ccaaacatct tactgaactc tccgatcgtg
     1501 tagctgtctt tcaaccggac actcctcctt gaaaatcaat tccttctctc attttatcag
     1561 cggttatcgc gttcatacgg gatccctaaa gcggaaggat cggagctttt ccctgccgaa
     1621 aagagaagaa caaaaacagc tgcgatataa ggcaaaatgg aaaaagcttg agaaggaatc
     1681 ggaatattca tcgtttggag ttggaattga acggcgtccg cgagcccgat caataaagcc
     1741 gaacaaagta ttcccaacgg gtttcttctg cctaatataa ccgtaaccaa agcaatatag
     1801 ccctttcctg cggtaatgtc ctccataaaa aatccgagct gagccaacgt aaaataggag
     1861 cccgccagcc ctcctaataa tccattcacc atacaagcca aataccgaat tcgaaacaca
     1921 ttgattcccg ccgtatcggc ggcactcgga tgttcaccga ccgctgtaaa tataactccc
     1981 cattccgtct ttttataaat aaaaaggaaa attaaaacca ataaatacat caggtaaac
//
LOCUS       DAOMIV010000404         2031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_63087_length_2031_cov_3.661943, whole genome shotgun sequence.
ACCESSION   DAOMIV010000404 DAOMIV010000000
VERSION     DAOMIV010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63087_length_2031_cov_3.661943"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1646
                     /locus_tag="PKV59_08490"
     CDS             <1..1646
                     /locus_tag="PKV59_08490"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HQP46824.1"
                     /translation="TATIVNPGSYLIQGILNDGQIVVNSENEGPIKLILNNVEITGSK
                     NAAIMIENADKAIIILAEGSTNILNDAPETANGATDENRADAALYSKADLSIYGSGSL
                     VVNGNYKEGISVKDGLIIGSGDITVQAVEDGIYGKDYLVVGDGRVTVTAGKDGLKSEN
                     EDDTSQGFIEIRGGEINITSDGDSISAGTDIRISGGTIHLSGGGGSSVNPNEEISGKG
                     IKASGTIQIDGGTISVDASDDDIHSDTKVIINDGSFSLASGDDGIHADNEIEINNGQI
                     LIKQSEEGIESADITINSGTIDITSKDDGINASDGSGEERMGLGGNDFFGGRGMGQRP
                     VDRGQAPENMDAVQNPIAPQSPDINNGENQSASSIMLTINGGLIIVNAGGDGLDSNGS
                     IGMSGGTVLVNGPTNSGNGALDYMGTFDLSGGTVVAAGSAGMAQGSSADSSQNSIMIN
                     LDAVQEANTLFSILDQNGNVVIAFEPVKQYQSVFLTTPVLKAGETYTVRLGGTCSGTE
                     QDHLYTDGTVIDGTVYTTFEMAEGTTQIGTFTGRGMMRK"
     gene            1859..1996
                     /locus_tag="PKV59_08495"
     CDS             1859..1996
                     /locus_tag="PKV59_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46825.1"
                     /translation="MKKIRGKQIDTKPVGVHDFNGYRLRGFSSASWRIAQYRFAECEK
                     G"
BASE COUNT          634 a          382 c          527 g          488 t
ORIGIN      
        1 cgacggcaac cattgttaac cccggaagtt atctgattca aggaattctg aatgacgggc
       61 agattgtcgt aaactccgaa aatgaaggac cgattaaatt gattttgaat aatgtagaaa
      121 taaccggttc aaaaaatgcc gcgatcatga ttgaaaatgc cgataaggca ataattattc
      181 tggctgaagg aagcacaaat attttaaatg atgcacctga aacagcaaat ggagcgacag
      241 atgagaacag agctgacgcg gctctttaca gcaaagcgga tttatccata tatggttccg
      301 gatcattggt tgtcaacgga aattataaag aaggaatttc cgttaaggat ggacttatca
      361 tcggcagcgg tgatataacg gttcaggcag tcgaagacgg aatctatgga aaagattatc
      421 ttgttgtcgg agatggacgg gtaactgtca ccgccggaaa agatggattg aagtcggaaa
      481 acgaagacga cacttcccag ggatttattg agattcgtgg aggagagatt aatataactt
      541 ctgacggaga ttcgatcagt gccgggaccg atataagaat cagcggcggt acgatccatt
      601 tatccggcgg tggcggaagc agtgtcaatc cgaatgagga aatttccggc aaagggatta
      661 aagcctcagg aacgatacaa atcgatggcg gtacaatatc cgtggatgcc tctgacgacg
      721 acattcactc cgatactaag gtaattatca acgacggcag tttttcgctt gccagcggag
      781 atgacggaat ccatgcggat aacgaaattg aaatcaacaa cggtcaaatt cttattaaac
      841 aatccgaaga gggaatcgaa agtgcggata taacgattaa ttcgggaacg attgacatta
      901 cttcgaaaga tgacgggatt aatgcctcgg acggaagcgg tgaagaaagg atggggctgg
      961 gtggaaacga tttctttggc ggaagaggta tgggacaacg gcctgttgac cgcggccagg
     1021 ctccggaaaa tatggatgcc gtacaaaatc cgattgcgcc tcaatcacct gacataaata
     1081 acggcgaaaa tcaatctgcc tcttccataa tgcttacgat caacggcggt ttaataattg
     1141 tcaacgccgg gggtgacgga ctcgattcga atggttcaat cggtatgagc ggcggaaccg
     1201 tattggtcaa cggtccgacc aacagcggaa acggcgcatt agactatatg gggacgtttg
     1261 atttatccgg agggactgtt gttgccgccg gcagtgccgg aatggcacag ggaagcagcg
     1321 cggattcgtc tcaaaattcg atcatgatta atctcgatgc ggtacaagaa gcaaatacct
     1381 tgttcagtat tcttgatcaa aacgggaatg tggtcattgc ttttgaaccg gtgaaacaat
     1441 atcaatccgt ctttttgacc accccggtat taaaggccgg tgagacttat acggtccggt
     1501 taggcggaac ttgtagtggt acggaacagg atcatctgta tacggatgga actgtcattg
     1561 acggtactgt gtatacaacg tttgaaatgg cagaaggcac aactcaaatc ggaactttta
     1621 ccggaagggg gatgatgcga aaataaaatc aaaacgagta tcatgatgca ctaaacagaa
     1681 aaagaaccac cggtgaaagc cggtggttct ttttctgttc ttatacgaaa agtaaaccat
     1741 tttcgcttat cgagacctta tcatctatct aaattaggat gaagggagaa aaaacagaac
     1801 aaaggttccg agtgtatttg tcgaacaact cggcatgaac ggtatgatcc cggagacgat
     1861 gaaaaaaatc agaggcaaac agatcgatac aaagccggtc ggtgttcatg atttcaatgg
     1921 ttatcgtctt cgcggctttt cttccgcttc ttggcgaatt gctcagtatc gttttgcaga
     1981 gtgtgaaaaa ggatagatgt tatgggaatt ttcagaaaaa aatataaatg g
//
LOCUS       DAOMIV010000405         2023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_63472_length_2023_cov_3.962907, whole genome shotgun sequence.
ACCESSION   DAOMIV010000405 DAOMIV010000000
VERSION     DAOMIV010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63472_length_2023_cov_3.962907"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1915
                     /locus_tag="PKV59_08500"
     CDS             170..1915
                     /locus_tag="PKV59_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46826.1"
                     /translation="MINRKLLLLFSITMVSLMLIAMPVLADEAKYFRTYFGSGDIPSI
                     DQALVTDFIGIQLVDEMTVGIMRQNETTGLLEPGMATEYSVSDDGRVYTFKLLQNVPW
                     VRYNPETKAVEEVKDCDGNVRYVTAHDFVYGAERTLRPETASGYAYVANLIEGAEAYN
                     TPVEEGEATPVARPDFSTVGIKALDDFTIEYTFKEAGVFNLNILSMWMLHAMPSWIID
                     GDACNEALGERWTETGFYQGYGPFTLEEWVHDSHLTIIANPFWPGTEFVPKPKIAGVH
                     YSLIDTSAALAEYEAGNMDEAGIPSADYDRITSDPALKDQIKSSTDSVGTEFLIYNEW
                     LPPTDDVRIRKALSLAVDKEAVVKTLKEGIAAPFYIHPGVDGAPKVEDYPDLGIMYDP
                     EAAKALIDEYCKEKGIQPKDIVVTYNFNTSESRKMLAETIQYMWQNTLGITVNLKNSE
                     WAVYKVERQQGLDNVYRSTWVQDYMDANNFTADVFLCGTGYSSVTDWPSKDCTDKSNP
                     AYIEYEEVVKKAGKETDPKARAALYARSDEIIIEEEAIINPIYWYSSLVLRKPNIIAP
                     ETLTGYAHWEKWEIK"
BASE COUNT          594 a          398 c          471 g          560 t
ORIGIN      
        1 gtaatctttt tatttctcaa taacaaatta tgacgaaaac cttgaaaaat aaccgttctt
       61 agattaatat cgtatttatc gttttatttt caaaagagtt tttaccattt tttaatgaca
      121 tatcgaaatt cggtatgaac tttatataga aacaaagaaa ggtgtgacta tgattaacag
      181 aaaattgctt ttattattca gcattacgat ggtttcgctc atgctgattg caatgcctgt
      241 cctcgccgat gaagcaaagt atttccgaac atactttggc agcggagata taccttctat
      301 tgaccaggct ttggtgaccg atttcattgg aattcaattg gttgatgaaa tgacagtcgg
      361 catcatgaga caaaatgaaa ccacgggatt attggaaccg ggcatggcaa ccgaatattc
      421 ggtttcggat gacggcagag tatatacatt taaattgctt cagaatgttc cctgggtccg
      481 ttataatccc gagacaaaag cagtggaaga agtcaaagat tgcgacggaa atgtgcgcta
      541 tgtgaccgca catgattttg tctatggtgc ggaaagaaca ttgcggccgg aaacagcatc
      601 gggatatgcc tatgttgcca atctgataga aggtgcggaa gcttataata cgccggtcga
      661 agaaggggaa gctacgccgg tagcccggcc ggatttctca accgtgggta ttaaagctct
      721 ggatgatttc accatagaat ataccttcaa agaagcgggc gtttttaatc ttaacatcct
      781 gagtatgtgg atgttacatg ccatgccttc atggattata gacggagatg cctgtaatga
      841 agctttgggg gaacgttgga cagaaaccgg tttttaccaa gggtatggcc cgtttacact
      901 ggaagaatgg gttcacgact cccatttgac aattatcgcc aatccttttt ggccgggaac
      961 ggaatttgtt cccaaaccga agatcgcagg tgttcactat tctctgatcg atacttccgc
     1021 tgccttagcg gaatacgaag ccgggaatat ggatgaagcc ggaattccca gtgccgatta
     1081 tgatcggatt acttctgacc cggctctcaa ggatcagatc aaatcttcga cggattcggt
     1141 cggaaccgag tttcttattt acaacgaatg gctcccgcca accgatgacg ttcgaatccg
     1201 aaaagcgtta agccttgcgg tagacaaaga agctgtagtc aagacattga aagagggaat
     1261 agcagcaccg ttttatatcc acccgggagt ggacggggcg ccgaaagttg aagattaccc
     1321 cgatttgggc attatgtatg atccggaagc ggcgaaagca ttgattgacg aatattgcaa
     1381 ggaaaaaggg attcaaccga aagatatcgt tgttacttac aatttcaata ccagtgaatc
     1441 caggaaaatg ctggctgaaa caatccaata catgtggcaa aatacattgg gcatcaccgt
     1501 aaacttaaag aatagtgagt gggctgtcta taaggttgaa cggcaacagg gtttggataa
     1561 tgtttatcgc agcacctggg ttcaagatta tatggatgcc aacaatttca ccgccgatgt
     1621 ctttttatgc ggcacaggtt attcttctgt aacagattgg ccgtcaaaag attgtacgga
     1681 taaatccaat ccggcttata tcgagtatga agaagttgtg aagaaagccg gaaaagagac
     1741 cgatccgaaa gcccgcgctg ctttatacgc ccgttccgat gaaattatta tcgaagaaga
     1801 ggcgattatt aacccgattt actggtatag cagtttggtt ctgcgcaaac cgaatatcat
     1861 cgctcccgaa acgttaaccg gttatgcaca ttgggaaaag tgggaaatca agtaattaga
     1921 gtttttcttg ctcataaaac agaaggctcc gatttatttt cggagctttc tttttattga
     1981 atgatcgctt gaaccatcac ttcttttttc cctttcacta cgg
//
LOCUS       DAOMIV010000406         2023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_63474_length_2023_cov_3.753557, whole genome shotgun sequence.
ACCESSION   DAOMIV010000406 DAOMIV010000000
VERSION     DAOMIV010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63474_length_2023_cov_3.753557"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKV59_08505"
     CDS             <1..373
                     /locus_tag="PKV59_08505"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQP46827.1"
                     /translation="PTEPLTIFLGALMGVLQSVIICTVGNTFASLIEYYIGSLIGDIS
                     DFEKQKENLPLKLGKMPVKSLPFQFGVRAIPGIGSKFASVSSGIYKVPIPFFLTISAI
                     SNFIGAIFLSYGGDFLARYFK"
     gene            393..560
                     /locus_tag="PKV59_08510"
     CDS             393..560
                     /locus_tag="PKV59_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46828.1"
                     /translation="MEFTLPVYHEPDFSQAMFVHSPNVNTEQVIKDGVAPDNFHGTSM
                     FPEYFKINGEW"
     gene            579..1511
                     /locus_tag="PKV59_08515"
     CDS             579..1511
                     /locus_tag="PKV59_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46829.1"
                     /translation="MDCCVVIRESGKLEVIELRNLKIGDRVILGRTDHAEEGIFLHNS
                     GFTVAEEINEKFLFRAGRSRETAFSKDYDSLYHLLLHEKEFGYIVWVMGPACAFDADS
                     RNAMSQLICNGYAHGLLAGNALATHDLEAAYFGTALGQDIYTQESKPNGHYHHIELIN
                     RVRRSGSIRKFIDENQINNGIIASCIRREIPIVLTGSIRDDGPLPEVIADVYQGQDAM
                     RNIIRKATTVICLATQLHTIATGNMTPCFRVVNGKVRPLYIYSVDISEFALNKLSDRG
                     SLTATSIVTNVQDFVVMVSNGLQKIEEKPFQSCW"
     gene            1699..>2023
                     /locus_tag="PKV59_08520"
     CDS             1699..>2023
                     /locus_tag="PKV59_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HQP46830.1"
                     /translation="MEIKTITIIGQGALGILFGSQLAKHLGNQNVRFLADRERIDRYQ
                     KEGIYCNGEKCDFTYITPDLQGNPADLLIFSVKYPALPQAICDAKNQVGDKTIILSLL
                     NGITSE"
BASE COUNT          589 a          416 c          441 g          577 t
ORIGIN      
        1 cccaaccgaa cctttaacaa ttttcctcgg agcattaatg ggtgtcttgc aatccgtcat
       61 tatctgcacc gtaggaaata cctttgcatc tttgattgaa tattatattg gaagtttaat
      121 cggagatatc tccgattttg aaaaacaaaa agaaaactta cccttaaagc tggggaaaat
      181 gcctgtaaaa tctctacctt ttcagttcgg agttagggct attccgggca ttgggtcaaa
      241 atttgcgagt gtttcaagtg gaatttataa agttcccatc cctttcttcc taacaatcag
      301 cgccatatcg aattttatcg gcgccatttt tctttcctac ggcggagatt tcttagcccg
      361 ttattttaaa taatttttca aaggagaaca tcatggaatt cactcttccc gtctatcatg
      421 aaccggattt ttctcaggcg atgtttgttc attcgcccaa tgtaaacacc gaacaagtga
      481 tcaaagacgg agtcgctccg gataattttc acggaacttc tatgttcccc gaatatttta
      541 aaatcaacgg agaatggtag cttgcggaag acagtcggat ggattgttgc gtagtgattc
      601 gcgaaagcgg aaaacttgaa gtaattgaac ttcgaaattt aaaaatcgga gatcgagtca
      661 ttctcggtcg aacggatcat gcggaagaag gtatatttct tcataattcc ggctttaccg
      721 tcgctgaaga gataaatgaa aaattcttat ttcgggcagg cagaagccgt gaaaccgcct
      781 tttcgaaaga ttatgattcc ctgtatcatc tgctgctcca tgaaaaagaa ttcggatata
      841 ttgtatgggt gatgggacct gcctgcgctt tcgatgccga ttctcgaaat gcaatgtcgc
      901 aattgatatg caacggttat gcgcacggtt tgttggcggg gaatgctctg gcgacacatg
      961 atttagaagc cgcttatttc ggaaccgcat taggtcaaga tatctatacg caggaatcga
     1021 aaccgaacgg tcattatcat catatcgaat taattaatcg tgtcagacgc agcggttcga
     1081 ttcgaaaatt tatcgacgaa aatcaaatca ataacggtat tatcgcctct tgtatccggc
     1141 gagagattcc gatcgttctg acgggatcca tccgagatga cggtccgctt ccggaagtta
     1201 tcgcggatgt ctatcagggg caagatgcca tgcggaacat cattcgaaaa gccacaaccg
     1261 tgatctgttt ggcaactcag ttgcatacga ttgccaccgg caatatgacg ccctgttttc
     1321 gggtggtaaa tggaaaagtc cgaccgcttt atatttattc cgtcgatatt tccgaatttg
     1381 cattgaacaa actgagcgat cgcggcagtc tgaccgccac cagcatcgta acaaatgtgc
     1441 aggatttcgt tgtaatggtc tcaaacggtt tacaaaaaat cgaagaaaaa ccttttcaat
     1501 catgttggta agccaagccg gagaacggaa agttatttta agcctcaagt agctttgtgc
     1561 caaggtctcg taatttccgg gcatttaatc attcccgttt ccggaatata atggagccat
     1621 catttcggtt atagaaaggc aacagtctga aggaattttt cattatttga acacttttga
     1681 caggaaacgg aaaaaattat ggaaatcaaa acaataacaa tcatcggcca aggcgctctc
     1741 ggtattctat tcggtagcca attggcgaag caccttggga atcaaaacgt aaggtttctg
     1801 gcggatcgag aacgtatcga tcgatatcaa aaagaaggga tttactgcaa cggggaaaaa
     1861 tgcgatttta cttatatcac tcctgatttg caagggaatc cggctgattt attaattttt
     1921 tccgtcaaat atcccgccct gcctcaggcg atttgcgatg ctaaaaatca agtgggggat
     1981 aaaacaatta ttctttcatt gctgaacgga ataaccagtg aac
//
LOCUS       DAOMIV010000407         2023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_63486_length_2023_cov_3.234248, whole genome shotgun sequence.
ACCESSION   DAOMIV010000407 DAOMIV010000000
VERSION     DAOMIV010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63486_length_2023_cov_3.234248"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="PKV59_08525"
     CDS             complement(<1..693)
                     /locus_tag="PKV59_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQP46831.1"
                     /translation="MKTLAIDFGNTNTVLAFRESSSREVQCIDLPGISEPGSVSIPSM
                     IHYGDGQTLIGSQVIREEKENSYNTFRWMKRYIGMNSPYHIRVNGEKISAKQAAEEFL
                     SNLIRETEERFSVHADELVFSVPVESFEYYANWLISCFTIDGFRKVRIVDEAVSAAAG
                     YGLKMHPGEPALFFDFGGSTMQATVVLLHESNGREGFLPESFQVIGKSGCSIGGSTVD
                     RWIFEEALMQNRR"
     gene            830..1972
                     /gene="galK"
                     /locus_tag="PKV59_08530"
     CDS             830..1972
                     /gene="galK"
                     /locus_tag="PKV59_08530"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HQP46832.1"
                     /translation="MLEKIINAFKNRFGADPDFVVRSPGRINLIGEHTDYNEGFVFPM
                     AIKYAQWLAIKANKTEHVSLYSADIDKDVVFSVTDFSQKITGWACYPQGIAWALHDNG
                     YPLKGWDGLLLGDLPLGAGLSSSAALDLVTARAFSLTGGFQWDPIQMSVISKQSENQW
                     IGLNNGIMDQLISATGEKGKAILIDCRSLDIQPHALPTDTIIVVMNTMVKHALVESGY
                     NDRFRQCMEGAKAFGKKFLRDVTMEEFNDGADRLDEVIRRRCRHVISENARTLEAAGC
                     MERNDPVRLGQLMNESHASLRDDFEVSCEELDIMAEEGQKQPGCYGARMTGGGFGGSA
                     IALVKTEYAEPFMKNIASIYEAKTGIKPNIFATDAERGTSVEYTKD"
BASE COUNT          552 a          465 c          468 g          538 t
ORIGIN      
        1 tcttcgattc tgcatcagcg cttcttcaaa tatccaacgg tctaccgttg atcctccaat
       61 ggaacaaccg gatttcccga tcacctgaaa agattccggc agaaaccctt cccgcccgtt
      121 cgattcatgt aagagaacga cggtagcctg catggtgctg cccccgaaat caaagaacaa
      181 agctggttca cccggatgca tttttaagcc atatccggca gccgccgaaa ccgcttcatc
      241 cacgatccgg actttccgga agccatcgat cgtaaaacag gaaatcagcc aattcgcata
      301 atattcgaag gattccaccg gtacggaaaa aaccagttca tcagcatgaa cggaaaagcg
      361 ttcctccgtt tcacggataa gattcgaaag aaactcttcg gcggcttgtt ttgcgctgat
      421 tttctcgccg ttcactcgga tatgataggg gctgttcatg ccgatatagc gtttcatcca
      481 gcgaaacgta ttataagaat tttctttttc ctctcggatt acctgacttc ctatgagagt
      541 ttgcccgtcc ccataatgga tcatggaagg aatcgaaaca cttccgggct cactgatacc
      601 cggcaaatca atacactgca cctcccgaga agaactttct cggaaagcaa gaactgtatt
      661 ggtattgccg aaatcaatgg ccaatgtctt cattcgttaa acaatctttt ctgcttcttt
      721 gaattcagtc gcttttgcct tatcccctgc caacctaatg ataaaataaa tcgatcaaag
      781 gaaaacgaat tgattatttt ttatcaaagc tgccgaaagg atcattgcca tgcttgaaaa
      841 aatcataaat gcatttaaaa accgatttgg agccgatccc gatttcgtcg ttcgttcacc
      901 ggggcgtatc aacctcatcg gcgaacacac ggattataac gaggggttcg ttttcccgat
      961 ggcaattaaa tatgcacaat ggttggcaat caaagcaaat aaaaccgaac atgtttccct
     1021 ttattctgcg gatatcgata aagatgttgt attctccgtt accgattttt cccaaaaaat
     1081 cacggggtgg gcttgttacc ctcagggcat cgcttgggcg ctgcatgaca acgggtaccc
     1141 tttaaaaggt tgggatggct tgctcttagg agatcttcct ctgggtgccg gcttatcctc
     1201 atcggctgct cttgatttgg ttaccgctcg tgctttctcc ctgaccggcg ggtttcaatg
     1261 ggacccgata cagatgtccg ttatcagcaa gcaatcggaa aatcagtgga tcggtttaaa
     1321 taatgggata atggatcaat tgatctcggc taccggtgaa aaagggaaag ccattctgat
     1381 cgattgtcgt tcattagata ttcaacccca tgctttaccg accgatacga ttattgttgt
     1441 catgaatacg atggtaaaac acgcattggt tgaaagcgga tacaatgatc gcttccggca
     1501 atgtatggaa ggcgccaaag ccttcgggaa aaaattcttg cgtgatgtga caatggagga
     1561 gttcaacgat ggcgccgatc ggttagatga ggtgatccgc cgtcgttgcc ggcatgttat
     1621 cagcgagaat gcccgtacgt tggaggctgc cggatgtatg gaaaggaatg atccggtccg
     1681 cctggggcag ttgatgaatg aaagtcatgc cagccttcgg gatgatttcg aagtttcttg
     1741 tgaagaattg gatattatgg cagaagaagg gcagaagcaa cccggttgtt acggcgcccg
     1801 aatgacaggg ggaggattcg gaggcagcgc catcgcactg gtaaaaactg aatatgctga
     1861 accgtttatg aaaaatattg cttcgattta tgaagcgaaa accggcatta aaccgaacat
     1921 atttgccacg gatgccgagc gcggcacatc cgttgaatat accaaagatt gattttctaa
     1981 attataaaac ggtcatcaag aacctttttc tttgtttgta gat
//
LOCUS       DAOMIV010000408         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_64118_length_2010_cov_3.640409, whole genome shotgun sequence.
ACCESSION   DAOMIV010000408 DAOMIV010000000
VERSION     DAOMIV010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64118_length_2010_cov_3.640409"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKV59_08535"
     CDS             complement(<1..655)
                     /locus_tag="PKV59_08535"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HQP46833.1"
                     /translation="MNTIWILIQFILAFALIVSLHEFGHFLMAKFFKVEVEEFGIGLP
                     PRAAKLFTWGGTDFTLNWLPFGAFVKPKNEFGDDISEGGFKAAKPWQQFLIYLAGPIM
                     NLLTAFVLYSVIILNIGSPDPSRVIIDQVESGSPAEIAGILPGDQIVQIAGKDIDSFE
                     VVTETVDQYLDQEIPIRVLRNGEYIDLKVTPLSNPPEGRGAMGIIITYPMVDYPFFEG
                     "
     gene            980..>2010
                     /locus_tag="PKV59_08540"
     CDS             980..>2010
                     /locus_tag="PKV59_08540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP46834.1"
                     /translation="MEGNGEASSFLNMVQLKHFYQRSPKTVFLILAIFIRVFFLLFMF
                     ASGFSLEKAENLNPDSETYIVPADSLLSDFKFLNSDKTYETNRTPGYPFVIAGAKLIA
                     GDQWKWIVIFFQYGMNILAVMVFYDLLSLLIRNQKAVNLATFLAVINLHDIYFANLIL
                     TDSLFQSIILIGIYFFVRFLLQFKTSFLVMAMACMSVAAFIRPSGYYLPFAVLIGVAV
                     ITFRNFKQLCKFSLIIFLIGITPLLCWQHRNYRVADFNGFSSITAINLYYYHCAGINA
                     VLNGTDFYTEQEKLMNNPELIELQKSMTLPAAEQKLAKEIISAHIPIYILLNIQGAGF
                     IMLFPGVLD"
BASE COUNT          547 a          406 c          384 g          673 t
ORIGIN      
        1 ccccttcaaa aaacggataa tctaccatcg gataggtgat aatgattccc attgctccgc
       61 gaccttccgg tggattgctc agcggagtca ccttcaaatc gatatattcc ccatttcgga
      121 gcactcgaat cggaatttcc tgatccagat attgatcaac cgtctctgta acaacttcaa
      181 aagaatcgat atctttaccg gcaatttgga caatttgatc tccggggaga attccggcga
      241 tttctgccgg agaacccgat tcgacctgat cgatgattac tctactcgga tccggtgacc
      301 cgatattaag aataatgacg ctgtatagta caaaagcggt caataaattc ataatcggac
      361 ccgcgagata gattagaaat tgttgccatg gtttagcggc tttaaatccg ccttcactta
      421 tatcatcgcc gaattcgttt ttcggcttga caaatgctcc aaatggcaac cagttcaggg
      481 tgaagtcggt tcctccccat gtgaaaagtt ttgctgcccg aggtggcagt ccgatgccga
      541 attcttcaac ttcgacttta aaaaattttg ccatcaaaaa atgtccgaat tcatgcaacg
      601 atacgataag tgcgaatgcc aagataaatt gtattaagat ccaaattgtg ttcatcctac
      661 tccataaata gcgacgtgct attttctttt attgctattt tcttaccgcc ggtccgattt
      721 ttgaaccggc agaatttata ttataaatct tgatttattg taccgtgaaa gtattagagg
      781 ataattaatg cgaaattaag atatcttttg gattgaactt ccttctgttc tcatggcaaa
      841 aggtctcaat ttgtggatcc gctggtaaaa ttgatccgga ataacggaaa agacaaacgc
      901 gaattaaaat ggagccggtt tctttttatc gatatttcat attggacttt tataaagtgg
      961 ccatgctcca aaaataatca tggaaggaaa cggagaagca tcctcatttt tgaatatggt
     1021 tcaattaaag catttctatc aaagatctcc caaaaccgtt tttttgatcc tggcgatttt
     1081 tattcgagta ttcttcctgt tatttatgtt cgcatcaggt ttttcattgg aaaaagctga
     1141 aaatttgaac ccggattccg agacctatat cgtcccggca gattctttat tatctgattt
     1201 caaatttctc aattccgata aaacatatga gacgaatcga acgcccggat atcccttcgt
     1261 tattgcagga gcaaagttga tcgcaggcga tcaatggaaa tggatcgtca tatttttcca
     1321 atatggtatg aatatcctgg ctgtaatggt tttttatgat ttgctctctt tattgatccg
     1381 gaatcaaaaa gccgtgaatc ttgccacatt tttggcggtc attaatcttc acgatattta
     1441 ttttgcgaat cttattttga ccgattcttt gttccagtcg attatactga tcggtatcta
     1501 ttttttcgtc cgatttcttt tgcaattcaa gacctctttt ctcgttatgg caatggcttg
     1561 catgtccgtt gccgctttta ttcggccttc cgggtattat ctgccttttg cggttttaat
     1621 cggcgtagca gttatcacat ttcggaattt caaacagctt tgtaagttct ctctcattat
     1681 atttttaatc ggaataaccc ctttgttgtg ttggcaacat cgaaattatc gggtggctga
     1741 ttttaatggt ttttcttcga tcactgcaat taatttgtac tattatcatt gtgcggggat
     1801 taatgccgtt ttaaacggga cggatttcta taccgaacaa gaaaagttaa tgaacaatcc
     1861 ggaattgatc gaacttcaaa aatcgatgac tttaccggct gccgagcaaa aattagccaa
     1921 agaaatcatc tcggctcata ttcccatcta tattttgttg aatattcaag gcgccggatt
     1981 tattatgctt ttcccgggtg tgttggatat
//
LOCUS       DAOMIV010000409         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_64280_length_2007_cov_2.966701, whole genome shotgun sequence.
ACCESSION   DAOMIV010000409 DAOMIV010000000
VERSION     DAOMIV010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64280_length_2007_cov_2.966701"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PKV59_08545"
     CDS             <1..330
                     /locus_tag="PKV59_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HQP46835.1"
                     /translation="VGDRFPFRYLTEEYGLEYYAAFPGCSAEVEPSASTVAFLIDKVK
                     TEKIPVVFYIEFSTHKIADAIADSTGAKTMLLHSAHNVSQEDIDSGITYLSIMTENAE
                     KLREALN"
     gene            388..1227
                     /locus_tag="PKV59_08550"
     CDS             388..1227
                     /locus_tag="PKV59_08550"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQP46836.1"
                     /translation="MSQLIHSIQNLFLDDLGKTNLLGKIILAILIYAVMKIVLLIINK
                     ALRRFSSHKFPKVPESKKITVFSALNSFVKFIIYFIAITFILDLFGINTSSIIATAGI
                     GGIAIALGAQTLVKDILSGLFILLEDQFNIGDYLTIGSVTGTVEEIGLRKIKVRDDDG
                     SITLIPNSLVQQVTNYSTHPIKINVKFPVPYAIKPDEIGAMIEKISEECRKEVDYFTA
                     APAFSGIDGLADSNYTVQVAAETISGNQGAGQQLIKQKILKEIQDRGIFSTTVDENSE
                     KAQ"
     gene            1313..1639
                     /locus_tag="PKV59_08555"
     CDS             1313..1639
                     /locus_tag="PKV59_08555"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HQP46837.1"
                     /translation="MKVIFHVDEPEKWQLALENVRNFLNAEPEAEIEVLANSAGVKFY
                     IQEASKNDLFAELVREGIRFAACNNALRKFGFRREELPEAVNVVPAGVVELAERQEKG
                     FAYIKP"
     gene            1828..>2007
                     /locus_tag="PKV59_08560"
     CDS             1828..>2007
                     /locus_tag="PKV59_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46838.1"
                     /translation="MKINYQETTNDLLTRIDIHNKYGSKDIDQWMLDTLDLKPGIKIL
                     DVACGGGKQCISFYKY"
BASE COUNT          585 a          399 c          482 g          541 t
ORIGIN      
        1 gtcggcgatc gattcccttt ccgttatctg acggaagaat acggattgga atattatgcg
       61 gcttttcccg gctgcagtgc ggaagtcgaa cccagcgctt cgaccgtggc atttctcatt
      121 gacaaggtaa aaacggaaaa aattccggtt gttttttata tcgaattttc cacgcacaag
      181 atcgccgatg cgattgcgga ttcgaccgga gccaaaacca tgctgctcca ttccgctcat
      241 aatgtctcgc aggaggatat cgattcagga attacctatc tcagcattat gaccgaaaat
      301 gcggaaaagc tgcgtgaggc gttaaactaa gctcagccct gccggtacag gtttcagctt
      361 ttcacttcag aacaggatcg gtatacgatg agtcaactga ttcattccat ccaaaattta
      421 tttcttgatg atctgggaaa aaccaattta ttgggaaaga tcatcttggc gattcttatt
      481 tatgccgtga tgaagatcgt tcttttgata attaataaag ctctcagacg tttttcatcc
      541 cataaatttc cgaaggtacc ggaaagcaaa aagatcaccg ttttttcggc tttgaacagc
      601 ttcgtcaaat tcattattta tttcatcgca attactttta ttttagattt gttcggtatc
      661 aataccagtt cgattattgc gacagccggg atcggtggta tcgccattgc attgggtgcc
      721 caaacattgg tgaaggatat cctgagcggg ctctttattc ttttggagga tcagttcaac
      781 atcggtgatt acctgaccat cggatcggtc accggtacgg tggaagaaat cgggttaaga
      841 aaaatcaagg tgcgggatga cgacggatcg attactttga tccccaacag cctggtgcaa
      901 caagtgacga attattccac ccatccgatc aaaattaatg ttaaattccc ggtcccgtat
      961 gccataaaac cggacgaaat cggcgccatg attgaaaaaa tatccgaaga atgccgaaaa
     1021 gaggttgact atttcacggc tgcccccgct ttttccggga tcgacggctt agcggattca
     1081 aattacacgg tacaagttgc cgcggaaacg atatcgggga atcagggtgc cggacagcaa
     1141 ttgatcaagc agaaaatatt aaaggaaatt caggatcgcg gcattttttc aaccaccgtc
     1201 gacgaaaatt ccgaaaaagc tcaatagcct tgaagttaag gacggatatc ggcagttccc
     1261 aaaggcagcc ggcgatgcgg accaaaattg tttgttccca aaaggagaaa aaatgaaagt
     1321 aatttttcat gtagatgaac cggaaaaatg gcaactcgct ttggaaaatg ttcggaattt
     1381 tctaaatgcc gaaccggaag cggaaattga ggtactcgcg aattccgccg gtgtcaaatt
     1441 ttatattcaa gaggcgtcga agaacgatct gttcgcggaa ttggtcagag aaggaatccg
     1501 ttttgcggct tgtaacaatg cattgcggaa gttcggattt cggagagagg aactgccgga
     1561 agcagttaac gtagttccgg cgggagtggt cgaactggct gaacgtcagg aaaaaggatt
     1621 cgcttatatt aaaccttgag ccggagaaat ttccggacgg ttccgggttt gttagctgcg
     1681 ccggaagagt ttttgaattc accgagacgg ccgttagggt tctccggtat ctttctatga
     1741 ttccgaaagg tgtatcagaa aatcttcttg aaatcgttat ttggtaaaat gggtaatatt
     1801 cattaaattt gaaaattggg agaaaaaatg aaaatcaatt atcaggagac gaccaacgat
     1861 ctgttgacca ggatcgatat ccataataaa tacggcagta aagatattga tcaatggatg
     1921 ttggatacac tggatctgaa acccggaata aagattttgg atgtggcttg cggcgggggc
     1981 aagcaatgta tttcatttta taaatat
//
LOCUS       DAOMIV010000410         2000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_64661_length_2000_cov_2.346530, whole genome shotgun sequence.
ACCESSION   DAOMIV010000410 DAOMIV010000000
VERSION     DAOMIV010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64661_length_2000_cov_2.346530"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKV59_08565"
     CDS             complement(<1..449)
                     /locus_tag="PKV59_08565"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQP46839.1"
                     /translation="MTRFCLVRHGQTDWNLEGRYQGQSDIPLNSTGIKQARLIAEILR
                     DEKFDAVYSSDLSRAIRTAEIITEHRDHLKINTDPRLREVRQGEWEGLTRDIIRDRYP
                     KIWEALIENPVSVRPPGGETVLEVQSRVNAALNDISALYPDGNILVVS"
     gene            complement(446..>2000)
                     /locus_tag="PKV59_08570"
     CDS             complement(446..>2000)
                     /locus_tag="PKV59_08570"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HQP46840.1"
                     /translation="IVSDSPWKDLLDVISDEKPQSVLIEWKDDRLTEGLPVNEVLSNS
                     LVNVLIFRSDLPIHYERTMVAVRGGPHAELALKVAMTLKPNHLDVLHISRSGASDDAP
                     YKGYLQILNQLPEINQRFIVTDDVSKTLFQEADRYDLVILGTTATNALNGPIIGTVAE
                     QMLRNSKSAVLITKIRQEKNPPIFDESAGYRAISVLVDKWFAENTFHSDEFADLDRLM
                     QLKESSNLTVSLAMPALNEEKTVGNVISTIKTALMDNVPLIDEMILVDSNSTDHTREI
                     AEDLGIKTYIHQQLLPESGANEGKGEALWKSLLVTSGDIIAWIDTDIVNIRPHFVYGI
                     IGPLLLDPSVKFVKGFYRRPLKIGSKMQADSGGRVTELTARPLLNLFYPELSGVIQPL
                     SGEYAGRRDALENAVFYSGYGVEIGLLIDIFEKYGLNAIAQVDLLERIHHNQELEALS
                     KMSFAIIQTVLHKLENRYERSIMDDVNKTMKLIRYNDGEYYLDIEEIAEKPRPPMISV
                     PAYRESHHK"
BASE COUNT          525 a          457 c          439 g          579 t
ORIGIN      
        1 gatacaacca agatgttccc atcgggataa agagcggaaa tatcatttag cgcggcgtta
       61 acgcggcttt gaacttccag gactgtttca ccgccgggag ggcggaccga aacaggattc
      121 tctattaaag cttcccagat cttcggatat cgatctcgga tgatatcccg agtgaggcct
      181 tcccattcgc cttgcctgac ttctcggagg cggggatctg tatttatttt taaatggtca
      241 cgatgttctg tgatgatttc ggctgttctt atcgcacgtg acaaatcact tgagtagaca
      301 gcatcgaatt tttcatcccg aagaatctcg gcaatcaaac gggcttgttt gatgccggtt
      361 gaatttagag gaatatcact ttgaccttga taacgacctt ccaaattcca gtccgtctgt
      421 ccgtggcgca ctaagcaaaa tcgagtcatt tatgatgtga ctcccggtaa gccggaaccg
      481 aaatcatcgg cggacgtggc ttttcggcga tttcttcaat atccaaatag tattcgccgt
      541 cgttataacg aatcagtttc attgttttat tgacgtcatc catgatcgaa cgttcatatc
      601 ggttttcaag cttatgcaat acagtctgaa taatggcgaa agacattttg cttaatgctt
      661 caagttcctg attatggtgg attctttcca ataaatcgac ttgggcaatc gcattcaacc
      721 cgtatttttc gaaaatgtcg atgagcaatc ctatttcgac tccgtatccg gaataaaaaa
      781 cagcgttttc aagcgcatcc cttcttcccg catattctcc ggacaacggt tgaatcacgc
      841 cggaaagctc gggatagaac aggtttaata agggacgtgc cgtcaattcg gtcactctac
      901 cgccgctatc ggcttgcatt ttactaccta tttttagggg acggcgataa aaccctttta
      961 caaattttac ggacggatct aacagcaagg gaccgatgat gccgtataca aaatgagggc
     1021 gaatattgac aatgtcggta tcgatccaag cgatgatatc tccagaagtg accaaaaggc
     1081 ttttccatag agcttcacct ttcccttcgt tagcgcctga ttccggaaga agctgttgat
     1141 gaatgtacgt tttaatcccc aaatcttctg caatttcacg tgtatgatcc gttgaatttg
     1201 agtcgaccaa aatcatctca tcaatcaacg gaacgttgtc catcaatgcg gttttgattg
     1261 ttgaaatgac attcccgact gttttttctt catttaaagc cggcatcgca agactgacgg
     1321 ttaaatttga ggattctttt aattgcatga gtcgatccaa atcggcgaat tcatcgctgt
     1381 gaaaagtatt ctcggcgaac catttatcga ccaaaaccga aattgcccga tatccggcgc
     1441 tttcgtcaaa aatcggcggg tttttctcct gtcgtatttt tgtgattaat accgcggatt
     1501 ttgaattccg taacatctgt tcggctaccg taccgattat tggtccgttc aaagcattcg
     1561 ttgcggtagt acccaaaatt acgaggtcat accggtcagc ctcttgaaat aacgttttgg
     1621 aaacatcgtc ggtgacaatg aatcgctggt ttatttcggg gagctggttc aagatttgaa
     1681 ggtatccttt atagggggca tcatcggatg ctccggaacg agaaatatga aggacatcca
     1741 agtgattcgg ctttaaggtc atcgcaactt tcaaagccaa ttcggcgtgc gggccgccgc
     1801 ggacagccac catcgtccgt tcgtaatgaa tgggcaagtc actgcgaaat atcaggacat
     1861 tcactaatga attggaaaga acttcattaa ccggcagccc ttcggtgagt ctgtcatcct
     1921 tccattcgat caaaaccgat tgcggttttt catccgagat cacatccaat aaatctttcc
     1981 agggtgaatc agaaacgatt
//
LOCUS       DAOMIV010000411         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_64829_length_1996_cov_4.034003, whole genome shotgun sequence.
ACCESSION   DAOMIV010000411 DAOMIV010000000
VERSION     DAOMIV010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64829_length_1996_cov_4.034003"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="PKV59_08575"
     CDS             complement(<1..1501)
                     /locus_tag="PKV59_08575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP46841.1"
                     /translation="MTEKSYSGNLFFLLLACILILGAFIFIKTTPSGIGLVSDSVNYI
                     NGARSIAEGKGYYRESGGGTIKPITNFPPLYSILLSVPILFGAGGIQAAWAFSLIFYL
                     LNITMIAILVRAVTKNGWLGLAGSLLFCASKPFLYFQVFAMSEPLFFFCTLMAFYLLW
                     LGFQTDSWVRWLLCGVFCGAAFLTRYVGVVSLAVAVGILLFLPPARVRRMKAIGWVLL
                     GSVPLMMSWLIRNLIVSGNVTNREAYFHPLSGEEIHTGIMIFWRWIYPERYQNVTTPV
                     SWMRLGIILIGIAGVLFLAGTFLLSVRKKDISNEVRMIWAFVLYLFVYLAVIFITITF
                     LDWSVNIEERILFPCLMVMLLTLLTLIGMFLRKKKIFPIIGLLAVYGFLLITSSQDLI
                     QFIPRFGEVGYGWAWQGWEDSPAMNLIKEMPADTVIYSNQPEAVSLIAGRGAYALLDP
                     IDPSTGLKRPGYDETMATIRNQVIQGEAVLVFFGINSWVEKDQANWITRL"
     gene            complement(1498..>1996)
                     /gene="mltG"
                     /locus_tag="PKV59_08580"
     CDS             complement(1498..>1996)
                     /gene="mltG"
                     /locus_tag="PKV59_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQP46842.1"
                     /translation="TFNQKLTREVAEGLQRKGLSVAEGVTLASIVSREAMSSEEYGLI
                     ASVFLNRYRLGMPFQSDPTVQYAIGWDETEKTWWKNPLASSDLLIQSPYNTYIYNGFP
                     PTPICNFNFEVLEAVANAPETDYYYFRMRCDGSPFHNFAETYAEHVANACTKEETATP
                     FEINQ"
BASE COUNT          626 a          490 c          408 g          472 t
ORIGIN      
        1 ataagcgggt aatccaattc gcttgatctt tctcaaccca tgaattgatc ccgaagaaaa
       61 ccaaaaccgc ttcaccttga attacctggt tgcgaattgt cgccatagtc tcatcatagc
      121 ccggccgctt gagtcctgtg gacggatcga tcggatccaa caaagcatac gctccgcgac
      181 cggcaatcaa actgaccgct tcgggttgat tggaataaat tacggtgtcc gctggcatct
      241 ctttgatcag gttcatcgcc ggagaatctt cccatccttg ccatgcccaa ccgtaaccca
      301 cttcgccaaa gcgcgggata aactgaatta aatcctgaga agaagttatc aacagaaatc
      361 catacaccgc caataaaccg ataatcggga agattttttt cttcctcaag aacatcccga
      421 ttaaagtcaa aagagtcaac aacatcacca tcagacaagg aaataaaatc cgttcctcaa
      481 tattcaccga ccaatccaag aatgtgatcg taataaagat aacggcaagg tagacaaaca
      541 gataaaggac aaaagcccaa atcattctca cttcattcga aatatctttt tttcgaaccg
      601 ataaaaggaa tgtccccgct aagaacaaga caccggctat cccgatcaaa ataattccca
      661 atcgcatcca tgaaacaggc gtcgtgacat tctggtatcg ctccggatat atccatcgcc
      721 aaaaaatcat gatcccggta tgaatctctt ctccggaaag cggatggaaa taagcctccc
      781 ggttggtaac attccctgag acaatcagat tccgaatcag ccagctcatc atcaaaggaa
      841 ccgaaccgag tagaacccat ccgatcgctt tcattcgccg aacgcgtgcg gggggaagaa
      901 agagcagaat tccgaccgcc accgctaatg acactactcc aacataacgg gtcagaaaag
      961 cggctccgca aaaaacccca cataacaacc accggaccca tgaatcggtc tgaaaaccga
     1021 gccatagcag ataaaaagcc attaatgtgc agaagaaaaa cagtggctcc gacatcgcaa
     1081 acacttgaaa atacaggaat ggcttggagg cacaaaatag aagagatccg gcgagtccca
     1141 gccatccatt tttggtcaca gcccgtacca atattgcgat catcgttata tttaataagt
     1201 agaaaatcaa gctgaatgcc caggcggctt gaattccgcc ggcaccgaac aggatcggta
     1261 cggaaagcaa aatggaatat aaggggggaa aattagtaat cggtttaatg gtccccccac
     1321 cgctctcacg ataatatcct tttccttcgg cgatcgatcg agcaccgtta atataattga
     1381 cggaatcact gactaacccg ataccggaag gcgtagtctt aataaaaatg aatgcaccga
     1441 gtattaatat gcatgccaat aatagaaaga acaggtttcc ggaataggat ttttcagtca
     1501 ttggttgatc tcgaacggtg tagcggtctc ttcttttgtg caggcattgg caacatgttc
     1561 tgcataggtt tcggcaaaat tgtgaaatgg tgatccgtca cagcgcattc taaaataata
     1621 ataatcggtt tcgggcgcat ttgccaccgc ttccaatact tcaaaattaa aattacagat
     1681 cggtgtggga ggaaatccat tataaatgta agtattatag ggcgattgaa tcaataaatc
     1741 ggagcttgct aatggatttt tccaccaggt tttctcagtt tcatcccatc cgatggcata
     1801 ttgaacggta gggtcacttt gaaaaggcat tcccaatcga tatcgattca agaaaaccga
     1861 agcgatcagt ccatattctt cgcttgacat ggcttcccgt gagactatgg atgccaaggt
     1921 tactccttcc gcaacgctta gtcctttccg ctgaaggcct tctgcgacct cccgagtaag
     1981 cttttgatta aaagta
//
LOCUS       DAOMIV010000412         1995 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_64884_length_1995_cov_3.198969, whole genome shotgun sequence.
ACCESSION   DAOMIV010000412 DAOMIV010000000
VERSION     DAOMIV010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64884_length_1995_cov_3.198969"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..557
                     /locus_tag="PKV59_08585"
     CDS             360..557
                     /locus_tag="PKV59_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46843.1"
                     /translation="MLIRFAYLMAPRNYLDPGTGSMLLQAIIAGIAVAGGFLFALKDK
                     IRRKLNKQEKAEQTDLEQDKK"
     gene            662..1918
                     /locus_tag="PKV59_08590"
     CDS             662..1918
                     /locus_tag="PKV59_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46844.1"
                     /translation="MGSGLYSRLIKEGFLISHEEIASEDLEEGAYKIIQPQKIPFISY
                     PYEWSFGEYKDAALVTLKIQRIALEYNMTLKDASAYNIQFQDANAKLIDTLSFDDYQE
                     GVPWVAYQQFCKHFLAPLLLMAEVDIRLSQLMRVYIDGIPLDLTSKLLHGKGGFTALQ
                     HITWHAKASEKHADDGKQNKPIREATVRKSALIALIEGLIRSTEKINLKKIQTEWGDY
                     YSETNYSEKAIRSKGQIITDYLHKIEPKTVWDLGANDGRYSKIAVQAGAEVVAFDIDP
                     IAVERNYQDCKKGQKHILPLVMDLTNPSPAIGFANQERGTITDRQIPDCIMALALIHH
                     LAISNNLPFEMISGWLAECCTYLIIEFVPKQDSQVQTLLKTREDIFTDYSESGFEASF
                     ERDFQITEKQPVEDSDRILYLLRTRK"
BASE COUNT          662 a          340 c          446 g          547 t
ORIGIN      
        1 gacttagaga ccatataaat cgatttttcc atcaatagtc ggagcttttt atgccttatg
       61 attgtaaaat ccggttgatt ttttaaaggt cttcgggcct tttattgtaa aaaatgtcat
      121 tagaatccgg acatatcaga ctaagtatat ggcaacttca atattcctca aattgaaatt
      181 tctcccattc gatgttttta aaaaggatat gcgatctgat ccggcttttg ggatctgaaa
      241 attttatata atccaacttt aatttttttt aggctacaat tttgtttata atttgaaacg
      301 agagaagcat tttaaggata aaatcaatta aatgccggtg agacggaagg gaaaaagaca
      361 tgttgatcag atttgcatat ttaatggctc cgaggaatta tctggatccg ggaacgggga
      421 gcatgctatt gcaagcgatt attgcaggga tagcggtagc gggtggattt ttatttgcgt
      481 tgaaggataa aatcagaagg aaactcaata aacaggaaaa ggcagaacaa acggatttgg
      541 aacaagacaa aaaatgaaag acagaggatc atttcgagat ccttccggat tcatatatca
      601 gaaggatcaa ataatctatc ggcaaatcaa tcgaagatat caaaagcaat acgaaacatt
      661 aatggggtca gggttatata gccgcctgat aaaagaaggg ttcctgattt ctcatgaaga
      721 aatcgcatcg gaagatttag aagagggcgc ctataaaatc attcaaccgc aaaagatacc
      781 ttttatctcg tatccatatg aatggtcttt cggcgaatat aaagacgcgg cactggttac
      841 tctaaaaata cagcggatag cgcttgaata taacatgaca ttgaaggatg ccagtgccta
      901 taacattcaa tttcaagatg caaatgcgaa gctgattgat accctgtcct ttgatgatta
      961 tcaggaaggg gtaccctggg tagcctatca acaattttgt aaacattttc tggcgccttt
     1021 actcttaatg gcggaagtag atattcgact gtctcaactg atgcgagttt atatagacgg
     1081 aatcccgtta gacttgacgt ctaagctgtt acatgggaaa ggcggattta ccgctcttca
     1141 gcatataacg tggcatgcga aagcttcaga aaaacacgcg gatgacggga aacagaataa
     1201 accgattcgg gaagccacgg taagaaaatc ggcactgatt gcgttgatcg aagggttaat
     1261 tcgatcgacg gaaaaaatta atctgaagaa aattcaaacg gaatggggag attattattc
     1321 cgagacaaat tattctgaga aagccatccg aagcaaagga caaatcatca ccgactattt
     1381 gcataagatt gagccgaaaa cggtttggga tttaggagca aatgatggta ggtattccaa
     1441 aatagcggtc caagccgggg cagaagtagt tgcttttgat atcgatccga ttgcagtgga
     1501 aaggaactat caggattgta agaaagggca aaagcatatc cttccgctgg tgatggattt
     1561 gacgaatccg agtccagcga ttggttttgc caatcaagaa cgagggacca tcaccgaccg
     1621 gcaaattccg gattgcatta tggcgcttgc gttgattcat catttggcca tctcgaataa
     1681 tttaccgttt gaaatgatat caggatggct ggcagagtgc tgtacttatc tcattatcga
     1741 atttgttcca aagcaggatt cacaagtaca aaccttactc aaaacgcggg aagatatttt
     1801 caccgactat tccgaatccg gatttgaagc atcatttgaa agagattttc aaataacaga
     1861 aaagcagcct gtggaagaca gcgacaggat tctgtatttg cttcggacac gtaaataagt
     1921 tagagaagaa gaattagaga ctgattttca aaattcgttg ataggatttt catgaaaaaa
     1981 atattaagtt atctg
//
LOCUS       DAOMIV010000413         1985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_65384_length_1985_cov_3.873057, whole genome shotgun sequence.
ACCESSION   DAOMIV010000413 DAOMIV010000000
VERSION     DAOMIV010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65384_length_1985_cov_3.873057"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKV59_08595"
     CDS             <1..493
                     /locus_tag="PKV59_08595"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HQP46845.1"
                     /translation="SGVAILLELAERLPKESDKNIWLVFFDGEDQGNIAGWDEWCIGS
                     TMMAQYLEKQDRRPDKVVVIDMVGDKDLQIFREKNSDKALTDEIWSTAEKEGLSNIFQ
                     DKEKYSIYDDHIPFLQIGIPAVDLIDFDYPAWHTLADDIQNVSSDSLAAVEQVLYAWL
                     TKK"
     gene            668..>1985
                     /locus_tag="PKV59_08600"
     CDS             668..>1985
                     /locus_tag="PKV59_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HQP46846.1"
                     /translation="MAIELSNEQKKIFDNVRNTLTELLAVASRSDVPEKDIETLQNTL
                     KSLEDKFLVVIVGEFNSGKSTFINALLNTDILETGVTPTTSLIHLIRYGEEQSSAPIE
                     NWGQLITLPVPLLQSISLVDTPGTNSIFSDHIILTNWFFPRADLVIFITSADRPYTES
                     ESRFLQAIREWGKKIIIILNKKDLNESPEELDRIVRFIQENANRQLKSDIPILTVSAK
                     TALRARKENNPELWKKSGFDQLEIFLQEKLNEKSRFKYKMDSALAIGERVANQTLQLL
                     ENEIKFYREDLNLADAIQEQTQVYRSEIEREINRSLSEIHAYFRQIKESGNEYFEKLF
                     KVKNIPNLIRKDKTKLEFQNEVLKSLPTDIERKTMEMVENIYLQEQRMTQYATNQIEK
                     RKTQFPGTAISSPELSERSQLLQKMQATIDEMLEKMEKDMAADIGMK"
BASE COUNT          665 a          405 c          416 g          499 t
ORIGIN      
        1 atccggagtt gccatcttgt tggaattagc tgaaaggctg ccgaaagaat ccgacaaaaa
       61 catctggctg gtattcttcg atggcgagga tcagggaaat atcgcaggat gggatgaatg
      121 gtgtatcggc tcgacaatga tggcccaata tctcgaaaaa caagatcgcc gaccggataa
      181 agttgtggtg attgatatgg tcggggataa agatttacaa atcttccgcg aaaaaaattc
      241 cgataaagca ttaacggatg aaatctggtc aaccgccgaa aaggaaggat tgagcaatat
      301 ttttcaagat aaagaaaaat acagcatata tgatgaccat attccttttc tgcaaatcgg
      361 aattccggct gtcgatttaa tcgattttga ttaccctgcc tggcacactt tagccgacga
      421 tattcaaaat gtgtcttccg atagtctggc tgcggttgag caagtgcttt atgcctggct
      481 cacaaaaaaa taaaccgaaa cataaaacag ttagattctc tttattcgct gcatccctat
      541 ttggcattat ttacgaataa acagagccgg ttttccgaag catagcgccg aattatgact
      601 catgaacaat gccgtttagc gaaatgctat ataattcctt cataacaggt gaaggaaaga
      661 ccaaaaaatg gcaattgagc tttcaaacga acagaaaaag atttttgata acgtccggaa
      721 tacattaacg gaattactcg ccgtcgcatc tcgcagcgat gtccccgaaa aagatatcga
      781 gacgctgcaa aataccttaa aatctctcga agacaaattc ttggtggtca ttgtcggaga
      841 atttaattcc gggaaatcaa catttatcaa tgcgttgtta aataccgata tacttgagac
      901 cggcgtcact cctactactt cattgattca tctgattcgt tacggagaag aacaatcttc
      961 tgctccgatt gaaaattggg gacaattgat taccctgccg gtcccattgc tgcaatcgat
     1021 cagcctggta gacactcccg ggacaaattc gattttttcg gatcatatca tccttaccaa
     1081 ctggttcttt ccaagagccg atctggtcat ttttattacc tctgcggatc ggccttatac
     1141 ggaaagtgag agccggtttc ttcaagcgat tcgggagtgg gggaagaaaa taattattat
     1201 tctgaataaa aaagatttaa acgagtctcc ggaagagctt gatcgaatcg tccgttttat
     1261 tcaagaaaat gcgaatcgcc agcttaaatc ggacataccg attctcactg tcagcgcgaa
     1321 aaccgcttta cgggcacgta aagaaaataa tccggaatta tggaagaaaa gcggcttcga
     1381 tcagctcgaa atctttctgc aagagaaatt aaatgaaaaa tccagattta aatataagat
     1441 ggactccgcc ttggcaatcg gtgagcgagt ggcaaaccaa accttgcaac tgctggaaaa
     1501 cgagatcaaa ttttaccggg aggatctcaa cttggctgat gccattcaag aacagacgca
     1561 agtctaccga tctgaaatcg aaagagaaat caaccgttct ctgagcgaaa tccacgctta
     1621 tttcagacaa atcaaggaaa gcggaaacga atatttcgag aaacttttta aagtgaaaaa
     1681 tattcccaat ttgatccgca aagataaaac caagttggaa tttcaaaatg aagttttaaa
     1741 atctctcccc acggatatcg aaagaaaaac gatggaaatg gttgaaaata tttatttgca
     1801 agaacagcgc atgacccaat acgcaaccaa ccagatagaa aaaagaaaaa cccagtttcc
     1861 gggaacagcc atttcctctc cggaactttc ggaacgttct cagctgttac aaaaaatgca
     1921 agccacgatc gatgaaatgc tggaaaagat ggaaaaagat atggcagcgg acatcggaat
     1981 gaaac
//
LOCUS       DAOMIV010000414         1985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_65400_length_1985_cov_2.866321, whole genome shotgun sequence.
ACCESSION   DAOMIV010000414 DAOMIV010000000
VERSION     DAOMIV010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65400_length_1985_cov_2.866321"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="PKV59_08605"
     CDS             <1..1706
                     /locus_tag="PKV59_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46847.1"
                     /translation="KPPFDAIGLHIYWGAAFPDMITQNIIDKRIVTGNMADYIRLFNE
                     SIIEIFEKEYPIWITEVGYSNYWLSELYASYNHYEESTLQAIGLIRTYTMLLSTPPVE
                     KVFWYTYSPDIEDVYVINDISLNVLRTLYLAINDTMPLGRFRVMDTLGKERNDLCDYR
                     FLQNDGQTISIFWSNDKEESSSSIKPPLSSSIYLFDIENKTESQGIISFNEGTKFYIL
                     NIPVFIVGKMDNNESIVVTNDDTPVVAENKPAVRPLIYTKEGNVYVYDPEKGVTRALT
                     FDGGLSGGILYYNAMLSPDSKYVGVELSDSFMILDLQGNILYSWEKYSKPDIYADTLV
                     GWDDQNYLYFTRTIGDCIFTDNEIIGPDHVELVHIDPKTGESETLTNLAKLSNASHAY
                     SIGDKISNDGKYISMINAACSIGISDERYYMNIETGDVIQESDYLKIYPDYDIQFPIE
                     EENNYMIPGRILSPDHNYVAYIRFKYSEYDGEYFVISEEADSRQLFIASTKDLQNKLF
                     IDYADDLFGWSPDQTKLAYIRKEPGAGGLYPPSTLYILDLTVGQIIQIDNGLGIKSSG
                     W"
BASE COUNT          601 a          381 c          389 g          614 t
ORIGIN      
        1 acaagccacc gtttgatgcg attggtttac atatttattg gggagccgct tttccggata
       61 tgatcactca aaatattatt gacaaaagga ttgttaccgg aaatatggcc gattatattc
      121 ggttgtttaa tgaatcaatt atagagattt tcgaaaaaga atatccgatt tggataaccg
      181 aagttggtta ctcaaattat tggctctctg agctttatgc ttcttataat cattatgaag
      241 aaagcacgct tcaggcaatc ggacttatcc ggacctatac aatgctctta tcaacgcctc
      301 cggttgaaaa agtattctgg tacacctatt ctccggatat tgaagatgtc tatgtaatta
      361 acgacatcag cctgaatgtt cttagaactt tataccttgc aataaacgac acaatgcctc
      421 tcggaagatt ccgtgttatg gatacccttg gaaaagagcg taatgattta tgtgattatc
      481 gattcttgca aaatgacgga cagaccatat ccattttttg gtcaaatgat aaggaggaat
      541 catcgtcatc gataaaaccg cctctgtcaa gttcgatata tcttttcgat attgagaata
      601 aaacagaatc ccaagggata atctctttta atgaagggac aaaattctat attcttaata
      661 ttcccgtttt tatagtcgga aagatggata acaatgagag cattgttgtg acaaatgatg
      721 acacacctgt tgtagcggaa aataaacccg ctgtccgacc tttgatttat acaaaagagg
      781 gaaatgttta tgtctatgat cctgaaaagg gggtaacccg ggcacttacc tttgatggcg
      841 gtctttccgg gggtatttta tactacaacg ctatgctttc ccctgattca aaatatgtcg
      901 gagtcgaatt aagtgattca tttatgatcc tcgatcttca gggaaatatt ctttattcct
      961 gggaaaagta ttccaaaccg gatatctatg cggatacgct tgtcggctgg gatgatcaga
     1021 attatctgta cttcacaaga acgatcggcg attgcatttt cactgataac gaaattatcg
     1081 gtcccgatca tgtggaattg gttcatattg atccgaaaac cggagaatcg gaaacattaa
     1141 caaatcttgc aaaattaagt aatgccagcc acgcatacag catcggagac aaaatcagta
     1201 atgatgggaa atatatctca atgataaacg cagcctgctc cattggcata tcggatgaac
     1261 ggtattatat gaatatcgag acgggtgacg tcattcagga atccgactat ctcaaaattt
     1321 acccggacta cgatatacaa tttcccattg aggaagaaaa taattatatg atcccgggaa
     1381 ggattctctc tccggaccat aattacgttg cctatatccg ctttaaatat tccgaatacg
     1441 atggtgaata tttcgttatt tctgaggaag ctgactcaag acaactgttc attgcttcaa
     1501 caaaagattt acaaaataaa ctgtttattg attatgcgga tgatttattc ggatggtcac
     1561 cggaccagac taagctggct tatatcagaa aggaacccgg tgccggcgga ttatatcctc
     1621 cgtcgacatt atacattctc gatcttacgg tcggccagat cattcagatt gataatggac
     1681 tgggtattaa atcttccggt tggtagttcc gtttcgattt gattttttcg tcttgaaaga
     1741 tgccttcagt ttttaatgat tttatccgtc gaacacgata acgagttcaa taaaaaggtc
     1801 ccggcttccg gattactcca agacaatata cgctggtaga tatacttttt ccaaatgaaa
     1861 tcaacgctct tttcccccgt attttcggcg tattcagagc gtttttttat ttttcccaac
     1921 tgtaaaactc gggaaaaatg atcgatcagc gtctttcatc tttcgttaaa agatcgataa
     1981 taact
//
LOCUS       DAOMIV010000415         1983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_65490_length_1983_cov_4.771784, whole genome shotgun sequence.
ACCESSION   DAOMIV010000415 DAOMIV010000000
VERSION     DAOMIV010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65490_length_1983_cov_4.771784"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="PKV59_08610"
     CDS             complement(<1..414)
                     /locus_tag="PKV59_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQP46848.1"
                     /translation="MKQNLSVSKKDFFNKETFSDYSLILIGSVIQSIGMAYFLIPARL
                     ISGGVSGLAQVINAHTGWPIGGMTLIGNIPLFIFGWRYLGKRKFAIRTVFSVVLFSFF
                     TDLLTRYQKNAYLTHDIFLNTLFGAVILGIGFGLVY"
     gene            707..>1983
                     /locus_tag="PKV59_08615"
     CDS             707..>1983
                     /locus_tag="PKV59_08615"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQP46849.1"
                     /translation="MNCSKKKKYILPLFILLFIVGCNRSQQAAPTAEIPSEAVTRGTV
                     HNSLILVGNVEASQSSELSWKTSGVVDSVSVKVGDKVESGQVLATLLTDSMPASSIAA
                     EADKISAEDNLEELLVSETAKATAYKNLRDAEVALRDAEKLAEGLNYPIADQSEIDAA
                     EKAMIAARDAYQNAVADYEGVKLRDYYDEERMKKYETLQNALTAYAQAYDLWLYYVNN
                     APEITKKVTAASAKEAQASYEAALKEFKTYQVSFVRKQDQADAEATLEDAVTAYNARS
                     LIADLTGTVTVVNTAAGDYVKKGQSGLRIDDLSTLYISLDISEVDVNDLKDGMKAEIT
                     LDAQPTKVYQGTVRYISAFASENDYNVTYRAVVAFEQPDELVKVGMTGEIKIILSEKP
                     DCLLVPSSAIREGDNGSYVDVVVNGTLTPVSVVT"
BASE COUNT          631 a          384 c          470 g          498 t
ORIGIN      
        1 atagaccagc ccgaatccga ttcccaagat gacggcaccg aacagggtgt tcagaaaaat
       61 gtcatgggtt aaataagcat ttttctgata tcgggtcagc aaatcggtaa aaaatgagaa
      121 aagtacaacc gaaaaaaccg tacgaatcgc aaatttccgc ttccccaaat aacgccaccc
      181 aaagataaaa agaggaatat ttccgatcag tgtcatacca ccaatcggcc aaccggtgtg
      241 agcgttgata acctgtgcca atccggagac tccgccgctg attaatcgtg ccggaatcaa
      301 aaaatacgcc atcccgattg attggataac actcccgatc aaaatcagag aataatcgga
      361 aaatgtttct ttgttaaaaa aatctttctt ggagacggaa aggttctgct tcatactttg
      421 attaaatcat agaaacctta aaaattgaag gaaatacgaa ggaataacaa aaggaaaagt
      481 cgaagatcac ctatcaaata ttctcggatc cgtcacttgc gtcatagttt taataaagat
      541 ttaattattc tattttttaa tgtgtcataa aatgagtgcc taagagaaaa attgattgag
      601 aataatgcga atttattttc gaaaaccgca acgtttcttt caattaaccg tagattaata
      661 tgttgcgtat agattatgaa caaaaagctg taactaagag aaaaaaatga attgtagtaa
      721 aaaaaagaaa tacattcttc ccctattcat cctccttttt attgtaggtt gcaatcgttc
      781 tcagcaggca gctccgaccg ccgaaattcc aagcgaagcg gtcacacgtg ggaccgtaca
      841 caattccctg attttagtgg gaaatgtgga agcttctcaa tcttctgaat tgagttggaa
      901 aacttccggc gtagttgatt cggtatcggt aaaggtgggg gataaagttg aatccggaca
      961 ggttctggca accttattga ctgactcgat gccggcttcc agtatagcag cggaggcgga
     1021 taagatcagc gctgaggata atttggagga attattggta tccgaaacgg caaaagcgac
     1081 ggcttataaa aatcttcggg atgccgaagt agctttacgc gatgcggaaa aactggccga
     1141 agggttaaat tatccgattg cagatcaaag tgaaatcgat gccgccgaaa aagccatgat
     1201 cgctgcccga gatgcctacc agaacgcggt tgcggattac gaaggtgtca aattacggga
     1261 ctattatgac gaagagcgga tgaagaaata tgagactctt caaaacgctt tgacagcgta
     1321 tgcccaggct tatgatttat ggctttatta tgtcaataat gcgccggaaa taacgaaaaa
     1381 agtgactgca gcatctgcga aagaggctca ggcaagttat gaagccgcat taaaggaatt
     1441 taaaacatat caagtctcct ttgtccggaa gcaggatcag gctgatgcgg aagcgaccct
     1501 ggaagatgcg gtcacggcgt ataacgcgcg atctttaatc gccgatttga cgggaacagt
     1561 gaccgtagtg aatacggctg cgggagatta tgtcaaaaag ggacaaagcg gcttgcgaat
     1621 cgacgatctg agtaccttat atatctcttt ggatatttcg gaagtggatg taaatgacct
     1681 gaaagacggt atgaaagcgg aaattacgct ggatgctcaa ccgacgaaag tctatcaagg
     1741 aacagtcaga tatatttccg cttttgcatc cgagaacgat tacaacgtca cctatcgtgc
     1801 ggtggtagct tttgagcaac cggatgaatt ggtgaaagtg ggcatgacgg gagaaatcaa
     1861 gatcatcctt tcagagaaac cggattgttt gcttgttccg agctctgcga ttcgggaagg
     1921 ggataacggt tcctatgttg atgttgtggt gaacgggaca ctgacgcctg tttccgttgt
     1981 tac
//
LOCUS       DAOMIV010000416         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_65707_length_1979_cov_4.050416, whole genome shotgun sequence.
ACCESSION   DAOMIV010000416 DAOMIV010000000
VERSION     DAOMIV010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65707_length_1979_cov_4.050416"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKV59_08620"
     CDS             <1..550
                     /locus_tag="PKV59_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46850.1"
                     /translation="VGIANGEGVYKMYPHELSGGMRQRIMIAMAVACDPKLIIADEPT
                     TALDVTIQAQILDLFRNLKEKINSSIMLITHDLGVIAEMADFVVVMYAGRIVEKGSAH
                     DIFLNPAHPYTVGLMASKPVVNKKVDRLYSIPGSVPSPIHMPNYCYFRDRCDKRFGRC
                     NDDYPPEFCLSETHRTNCYLYE"
     gene            656..1624
                     /locus_tag="PKV59_08625"
     CDS             656..1624
                     /locus_tag="PKV59_08625"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HQP46851.1"
                     /translation="MNDAAFFEVKNLKKYYPIKSGFFNKVSGFVKAVDGISFTIEKGK
                     TMGLVGESGCGKTTVGKTILRLNSKTAGDVLYKGRELFELDAKEMRKLRPKMQIIFQD
                     PYSSLSPRLPVGEIIGEAVREHKIVPEDEFDDYITRIMIACGLQEYHKDRYPHEFSGG
                     QRQRICIARALALSPEFVVCDEPVSALDVSIQAQIINLLEDLQHQFNLAYLFISHDLS
                     VVQHISDTVAVMYLGNIVEFSDRDEIFSNPLHPYTKALFSAIPIPDPTIRRNRIILQG
                     SIPSPANPPKGCKFHTRCAYAMKKCETVEPVLRDLGNGHTVSCHLF"
     gene            1635..1913
                     /locus_tag="PKV59_08630"
     CDS             1635..1913
                     /locus_tag="PKV59_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46852.1"
                     /translation="MKNRTQNRDEIDQDDGRTVINMNVDGMPWFRDQNTEISQVPSTE
                     NQIDSHHQIEWKESFLMSKYVLKASLWIIACYSIGIILVILFLIKIWS"
BASE COUNT          588 a          371 c          423 g          597 t
ORIGIN      
        1 agtcggtata gcaaatgggg aaggtgttta caaaatgtat ccgcacgagc tttccggcgg
       61 gatgagacaa agaattatga ttgcgatggc cgtcgcttgt gatccaaaat taattattgc
      121 ggatgaaccg actactgctt tagatgtaac cattcaggca caaattttag atctgtttcg
      181 gaatttaaaa gagaagatca acagttcgat tatgttaatc actcacgatc tcggagtcat
      241 tgccgaaatg gcagattttg tcgtagtgat gtatgcgggt agaattgtgg agaaagggag
      301 tgctcacgat atttttttaa atcccgctca tccttatacc gtcgggttaa tggcttccaa
      361 accggttgtt aataaaaaag tagaccgcct ttacagtatt ccgggttcgg tccccagtcc
      421 gattcatatg ccgaattact gttatttcag agatcgatgc gataagagat tcggtcgttg
      481 taacgacgat tatcctcccg aattctgcct ttccgaaaca cataggacga attgttatct
      541 ttatgaatga tcttttgata attcaaataa ttttttcgaa ccgcgaattt cctttttttt
      601 atatttcttc attttgttca aaatatcaat cagaaagaga aggaaatcaa ctactatgaa
      661 cgatgccgct tttttcgaag taaaaaattt aaaaaaatat tacccgatca aatccggttt
      721 ctttaacaag gtaagcggtt ttgtaaaagc ggttgacggg attagtttca caattgaaaa
      781 agggaagacg atgggtttgg tcggagaatc agggtgcgga aaaaccacgg tcgggaaaac
      841 tattttgcgc ttaaacagta aaaccgcggg agacgtcctt tataaaggac gggaattatt
      901 tgaactggat gcgaaagaaa tgagaaaact gcgacccaaa atgcaaatta tttttcaaga
      961 tccatattca agcctttctc cacgcctccc tgtcggagaa attatcggag aagcagtcag
     1021 agagcacaaa atcgttccgg aagatgaatt tgacgattat attacaagga taatgattgc
     1081 ttgcggttta caggaatatc ataaagatcg ttacccgcat gaattttccg gcggccagcg
     1141 ccagcgtatt tgtattgccc gtgccttagc gctgagcccg gaatttgttg tttgcgatga
     1201 accggtatcg gctttggatg tgtctatcca agctcagatt atcaacctgc tcgaggattt
     1261 acaacatcaa tttaatttgg catatttgtt catttctcat gacttatctg tcgtacaaca
     1321 tatttccgat acggttgcag taatgtattt gggaaatatt gtggaattct ccgatcgaga
     1381 tgaaatattt tcaaatccgt tgcatcccta tacgaaagca ttattcagtg ccatcccgat
     1441 tccggatccg acgatcagac ggaatcgtat tattcttcaa gggagtattc cttctccggc
     1501 aaatccccca aaggggtgca aatttcatac acgttgcgca tatgccatga aaaaatgtga
     1561 aacggtggaa ccggtcttaa gagatttggg caacggacat acggtgtctt gtcatttgtt
     1621 ttgatggagt tgatgtgaaa aatcgaacac aaaatcgtga cgaaatagat caagatgacg
     1681 gcagaaccgt tattaatatg aatgttgatg gaatgccttg gtttcgagat caaaatacgg
     1741 aaatcagcca agtcccttcc acagaaaatc aaatcgactc ccatcatcaa atagaatgga
     1801 aagagtcttt tctaatgtcg aaatatgtcc tgaaagcaag tttatggatc attgcttgct
     1861 attcaatcgg tatcattctg gttattttat tcttaattaa gatctggtct taacttgatc
     1921 cgttttcttc tcatccggtg atgctgtaat atcatcagac aaagattttg ctctttcgg
//
LOCUS       DAOMIV010000417         1979 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_65711_length_1979_cov_3.579002, whole genome shotgun sequence.
ACCESSION   DAOMIV010000417 DAOMIV010000000
VERSION     DAOMIV010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65711_length_1979_cov_3.579002"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PKV59_08635"
     CDS             <1..474
                     /locus_tag="PKV59_08635"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46853.1"
                     /translation="AFAALGDGKQAGHLFELLNPIFHSDNEEKATVYRVEPYVICADI
                     YSQPPFTRRGGWTWYTGSAAWMFRLGIEAILGFHKIGNSLQIDPVIPPEWDEFEIQYR
                     FGKSTYFILVRNPDHVSRHVRTVQSDGKQIDPMSIPLVDDGLIHRIEVTMGEIKQ"
     gene            complement(587..1933)
                     /locus_tag="PKV59_08640"
     CDS             complement(587..1933)
                     /locus_tag="PKV59_08640"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46854.1"
                     /translation="MTQIKWFFSNNAVQWQQKTPTCFTGEWNLSTDGREYQKMFGFGG
                     CFNEIGFQTLKQLSDTEQKDVMKDLFYPSENGCNFSLCRLPIGANDYSFDWYSLDETP
                     GDYELADFSIERDKKALIPYIRAALAYLPDLNLFASPWSPPTWMKHPPVYNWGKLIFT
                     KEIQTAYAFYFVKFVEEYQKMGIKIDQIHVQNEPVANQKFPSCMWTGTELRDFIRDYL
                     GPAFRKAGLSTEIWLGTINAPGCDYQKMIFDKWADEDYDFFANTVLRDDSARQYISGV
                     SYQWGGKIAIQRTFESWWPRIRLMQSENECGFGDNTWKNALYNWTMMKHYIGNGAESY
                     IYWNMILPPDGVSTWGDPQNALITVSPTGPKIIKNPDYYLMKHFSSFIKRGAIRLGIQ
                     GDMAADSLIFHNPDGSFVIEAMNPFETELILKLNLSGIEAGFELEPKSLNSIIIDG"
BASE COUNT          561 a          438 c          376 g          604 t
ORIGIN      
        1 gcatttgccg ctctgggaga cggaaaacaa gcaggccacc tttttgaatt actcaacccg
       61 atttttcatt cggataacga agaaaaggca accgtttatc gtgtggaacc ttatgtcatt
      121 tgtgccgata tttatagcca accgcctttc acacgacggg gtgggtggac ttggtatacc
      181 ggttctgcgg cttggatgtt ccgattggga attgaggcaa ttctcggatt ccataaaatc
      241 gggaattcgc tgcaaatcga tcccgtcatt cctccggaat gggatgagtt tgaaattcaa
      301 tatcgtttcg gaaagtcaac ctatttcatt cttgttcgga acccggacca tgtctctcgg
      361 cacgtccgca ccgttcaatc ggacggaaaa caaattgatc ctatgtcaat accgttggtt
      421 gatgacggct taattcatcg gatagaagta acaatgggtg aaatcaaaca gtaatcaaag
      481 aaaccccacc atttgatggg gttcttttgt taaaccaatg atttatattg taatcagatc
      541 tattttgtaa tattactttc ggagaatcat aactgtaaac aaagtgtcaa ccatcaatga
      601 tgatactgtt caatgatttc ggctccaact caaatccggc ttcgattccc gaaagattca
      661 gctttaagat taattcggtt tcaaacggat tcatcgcttc gatgacaaag cttccgtccg
      721 gattatgaaa aatcagactg tctgctgcca tatctccttg aattcccaac cggatcgctc
      781 ctcttttaat aaatgatgaa aaatgtttca tcagataata atccggattc ttaattattt
      841 tcggcccggt cggactgacg gtaatgagtg cgttttgagg atctccccaa gtgctcacgc
      901 cgtccggagg caaaatcata ttccaataga tatagctttc cgccccgttc ccgatataat
      961 gcttcatcat cgtccagtta tagagagcat ttttccaagt attatcaccg aaaccgcatt
     1021 cgttttccga ttgcatcaat cggattctcg gccaccaact ttcaaaagtt ctttggattg
     1081 cgattttccc gccccattga taactgacac cgctgatata ttgcctggct gaatcgtcac
     1141 gtaaaacggt gtttgcaaaa aaatcatagt cttcatcagc ccatttatca aaaatcattt
     1201 tctggtaatc acatccggga gcattgattg ttcccaacca gatttcggtt gataagcctg
     1261 cttttcggaa agcgggtcca agataatcgc gaatgaaatc acgcagttct gttcccgtcc
     1321 acatacaaga agggaatttt tggtttgcca caggttcatt ttgtacatga atctgatcga
     1381 tttttattcc cattttttga tattcttcaa caaattttac aaaatagaag gcataggcag
     1441 tctgtatttc ttttgtgaaa atcaattttc cccaattata tacaggggga tgtttcatcc
     1501 atgtcggcgg actccaagga gaagcaaaca gattcaaatc gggcaaatag gcaagcgccg
     1561 ctcggatata tggaatcaat gctttcttat cgcgttcaat cgaaaaatcc gccaattcat
     1621 aatcaccggg tgtttcatca agactatacc aatcaaacga ataatcattt gcaccgatcg
     1681 gcaaacggca taaagaaaaa ttacacccgt tttcggaagg atagaataaa tccttcatga
     1741 catctttttg ttcggtatcg gaaagttgtt tcaatgtttg gaaaccgatt tcgttgaaac
     1801 atcctccaaa accgaacatt ttttgatatt ccctaccatc tgtcgacaga ttccattccc
     1861 ctgtaaaaca ggttggtgtt ttttgttgcc actgaacggc attatttgaa aagaaccatt
     1921 taatttgagt catttcttcc ctttattttt ccgaaccggc tgttaaccca ttaataata
//
LOCUS       DAOMIV010000418         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_65752_length_1978_cov_4.152366, whole genome shotgun sequence.
ACCESSION   DAOMIV010000418 DAOMIV010000000
VERSION     DAOMIV010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65752_length_1978_cov_4.152366"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1423
                     /locus_tag="PKV59_08645"
     CDS             161..1423
                     /locus_tag="PKV59_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQP46855.1"
                     /translation="MDEDGTGVLWVNANQTFYLNQSAALMTWCLINDFTDEEDRRLLK
                     KYYPDQYEQVTKDFDAFSPALKKMLRGENNVCDLCQSGIDSDIPFSANPSAPYRMDLA
                     LTYRCNNNCSHCYNDKSRQVKELPSKSWHKILEKIAQEGIPHIVFTGGEPTLYPDLID
                     LAEYAENLGLVAGLNTNGRKLRDAELMASLVRSKLDHIQITIESDQESIHDAMVQVPG
                     AWKETVAGIQNAVKSGIFTMTNTTLLQSNGTEENVRQLINFLAELGVQTVGLNALIYS
                     GEGKNVHTGLAESDLPALLNLALQTCENNGQQLIWYTPTQYCYFDPQDFQLGVKGCSA
                     ARYSMCVEPDGTVLPCQSWYEGVGNILTDPWQKIWNHPLCNEIRERKRMPESCNICEK
                     SQICGGGCPLAVDYKPPVKPQASIPSCF"
     gene            1441..>1978
                     /locus_tag="PKV59_08650"
     CDS             1441..>1978
                     /locus_tag="PKV59_08650"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQP46856.1"
                     /translation="MDTDLSALNLRFEQQAKWTFDFQRKLFHYRRTPPPWKILEVGCG
                     TGAVLRQMGQMGSVNDIFYGIDSDRVSLSFARTSEEGHFCNAFGSDLPFPNESFDLVF
                     CHYFLLWVKNPVMILKEMARVCKPGGMVVAFAEPDYEARIAYPPELDSPVRLQTDELI
                     RQGINPTAGRQLSSWMENA"
BASE COUNT          563 a          427 c          467 g          521 t
ORIGIN      
        1 gcgcctgtgc gggttgtgcc tgtgcttgtg ccggtggcgg aggaggaaga taaaaatggg
       61 tatttcgaat ctcttcggct tcaacaaaca attccacaaa gggttatatc agaaaaaggt
      121 aaaggacgag agcggctttg aaaacaggat ccatttaaga gtggacgaag acggcaccgg
      181 tgtactctgg gtaaatgcaa atcagacttt ttatttgaac cagagcgctg ccttaatgac
      241 atggtgtctt atcaatgatt ttacggacga ggaagaccgc cgcctgctga aaaaatatta
      301 ccccgatcaa tatgaacagg tgaccaagga ttttgatgcc ttttcacccg ccctaaagaa
      361 aatgctgcgg ggtgaaaata acgtctgtga tctttgtcaa agcggaatcg acagcgatat
      421 acctttttcc gcaaaccctt ccgctccata tcggatggat ttagctctca cctaccggtg
      481 taacaataac tgttcacatt gttataacga caaatccaga caagtcaaag aactcccttc
      541 aaaatcttgg cataaaattc ttgaaaagat tgcacaagaa ggaattccgc atattgtttt
      601 taccggcggc gaaccgacgc tttatccgga tttgattgat ctggctgagt atgccgaaaa
      661 tctgggtttg gtagccggat tgaatacaaa cgggagaaag ctgagagatg ccgaattgat
      721 ggcatctctt gtaagaagca aactggatca tattcaaatt accattgaat cggaccaaga
      781 atccatccat gatgcgatgg tgcaggtccc gggtgcttgg aaagaaaccg tagccggaat
      841 tcaaaatgca gtaaaatccg gcatttttac catgacaaat actactttgc ttcaatcaaa
      901 cggaacggaa gaaaatgttc ggcaattaat taattttctt gcggaattag gcgtacaaac
      961 cgtcggattg aacgctttaa tttattccgg cgaagggaaa aatgtccata caggccttgc
     1021 ggaatcggat ttaccggcac tgctaaattt agctcttcaa acttgtgaga ataacggaca
     1081 gcaactgatc tggtatacac ccactcagta ctgttatttt gatccccaag atttccaatt
     1141 aggcgttaaa ggttgttctg ctgcaagata cagtatgtgt gtcgaaccgg atggcactgt
     1201 cctcccctgc caatcatggt atgaaggggt cggaaacatt ttgacggatc catggcaaaa
     1261 aatttggaat catccgttat gcaacgaaat tcgtgaacgg aagagaatgc ccgaaagctg
     1321 caatatctgc gagaaatcgc agatctgcgg aggaggttgt cctttagcag tggattacaa
     1381 gcctccggtt aaaccccagg cttccattcc cagttgcttc tgattccgaa aatatttgaa
     1441 atggataccg atttgtctgc cttaaatctc cgtttcgaac aacaggcgaa atggactttt
     1501 gatttccagc gaaaattgtt tcattaccgg cggacaccgc cgccctggaa aattctggaa
     1561 gtcggctgcg ggaccggagc cgtccttcga caaatgggac aaatgggatc cgtaaatgat
     1621 atcttttacg gtatcgattc cgacagagtg tcattgtctt ttgctcgcac atcggaagaa
     1681 gggcattttt gcaacgcatt cggctcggat cttccgttcc caaatgaatc ttttgatctg
     1741 gtattttgtc actatttcct gctatgggta aaaaatcccg taatgattct gaaagaaatg
     1801 gcacgcgttt gtaaacccgg cggaatggtc gtggcttttg ccgagccgga ttatgaagcg
     1861 agaatcgctt atcctcctga actcgattca ccggttcgtc tgcagacaga tgaattaatt
     1921 cgacaaggca tcaatccgac ggcaggcagg cagctttctt cttggatgga aaatgccg
//
LOCUS       DAOMIV010000419         1975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_65908_length_1975_cov_4.856250, whole genome shotgun sequence.
ACCESSION   DAOMIV010000419 DAOMIV010000000
VERSION     DAOMIV010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65908_length_1975_cov_4.856250"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..732
                     /locus_tag="PKV59_08655"
     CDS             94..732
                     /locus_tag="PKV59_08655"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQP46857.1"
                     /translation="MKKNPTLDYEKALWEQGFFYVAGIDEAGRGAWAGPVMAGAVIFP
                     VDENLPVILDGVRDSKQMTPETREEKFRIITDYAIGWAVGSASNWEIDSMGILPATRL
                     AMDRAIAQLQPLPQYLLIDYVRLFRQMMPQKSIIKGDECSYSIAAASILAKVCRDHWM
                     VEHCAEEFPQYGFEHHKGYGTQFHQEALEKFGPCKYHRKSFHPIASELKLFE"
     gene            complement(794..>1975)
                     /locus_tag="PKV59_08660"
     CDS             complement(794..>1975)
                     /locus_tag="PKV59_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQP46858.1"
                     /translation="PIAGQYRLIDIPISNCINSGIFKIAVLTQFNSVSLHRHILDTYQ
                     FDRFHSGWVQIWAAEQRTDNNDWYQGTADAVRKQLSEIISSRTEYVLILSGDHLYRCN
                     YADMLQFHLDHHADVTIATQPVHKDEADRFGILKRDSEFKINDFKEKPKDPEVLKELV
                     SLDEEERPYLGSMGIYLFNKDLLVGLLNALDYSDFGKHIIPYAIEKYDVYGWTFDDYW
                     EDIGTIRSFYETNLMLTEDHPKFNFYDPKHPIYTLNKTMPTTKIENTEVKNALIANGC
                     MIKANSIEHSIIGIRSMIDVGTTIKDSILMGVDDDVFEKSANDSKYFDIPLGIGKNCH
                     IEQAIIDKNARIGDNVIIKPFPPHIELDDADPIKKYYVRDGIVIIPKNTSIKSGTVIT
                     P"
BASE COUNT          556 a          406 c          438 g          575 t
ORIGIN      
        1 caaagttcat gtgctgaatg attcagtcaa gatgtttcgg gaactattgc aaatacgccg
       61 caacgataaa tccggaattt ataagatcga acaatgaaga aaaatccgac tctcgattat
      121 gaaaaagctt tgtgggaaca aggctttttt tatgtggcgg gaatcgatga agccggtcga
      181 ggagcttggg ccggtccggt tatggcggga gcggtcattt tcccggtgga tgaaaatctg
      241 cctgttattt tggacggcgt gcgtgacagt aaacaaatga cgccggaaac tcgggaagaa
      301 aaattccgga taattaccga ttatgcgatc ggctgggcag tgggatccgc ttcaaattgg
      361 gaaattgatt ccatgggaat tttgccggct acacgactgg caatggaccg agcaattgct
      421 caacttcaac cgctgccgca gtatttactg attgattatg tccgattatt ccgccaaatg
      481 atgccgcaga aatccatcat caaaggagat gaatgctctt acagtatcgc ggcggcttct
      541 atcctggcaa aagtttgccg ggatcattgg atggttgaac actgtgccga agaatttccg
      601 caatatggat tcgaacacca taaagggtac ggtacccagt ttcatcaaga ggctttggaa
      661 aaattcggtc cctgtaagta tcatcggaag tcatttcatc ccatcgcttc cgaattaaag
      721 ttatttgagt gaaataaatt tcagaaaaaa acgggcgctt caagcgtccg ttttttctga
      781 tcaaaagttg tatttacgga gtgattaccg tgccggattt tatactggta ttcttgggaa
      841 taataacaat cccgtcacgt acataatatt tcttaatggg atccgcatcg tccaattcaa
      901 tatggggcgg gaagggttta atgatgacat tatcaccgat acgggcattt ttatcaatta
      961 tagcttgctc aatatggcaa tttttgccga ttcccaatgg gatatcaaaa tactttgagt
     1021 cgttggcact cttttcgaag acatcatcat caacgcccat caaaatagaa tctttgattg
     1081 ttgtcccgac atcaatcata ctgcgtatac cgataatgga atgttcgata ctgttcgctt
     1141 taatcatgca gccgttcgca atcagggcgt ttttaacctc ggtattttca attttcgtcg
     1201 tgggcatcgt cttgtttaag gtataaatcg gatgtttcgg atcgtaaaaa ttgaatttcg
     1261 gatgatcttc cgtcagcatt aaatttgttt catagaatga gcgaattgtc ccgatgtctt
     1321 cccagtagtc atcaaaggtc cagccgtaaa catcatattt ttcaattgca taagggataa
     1381 tatgcttgcc gaagtcggaa tagtccagtg cattcagtaa gccgaccaac agatctttgt
     1441 tgaacagata aattcccata gagcccagat aaggtcgttc ttcttcatcc agactgacca
     1501 attccttaag aacttcggga tctttgggtt tttccttgaa atcattgatt ttgaattcgg
     1561 aatcacgctt caaaataccg aagcgatccg cttcatcctt atgcacgggt tgagtggcga
     1621 tcgttacatc cgcatggtga tctaaatgaa attgaagcat atccgcgtaa ttacaacgat
     1681 acagatggtc accggataag atcagaacat attccgttcg cgatgagata atctcactca
     1741 attgttttcg aaccgcatct gcggttccct gataccaatc attgttatct gtccgttgtt
     1801 ccgctgccca aatttgaacc catccggaat gaaaccgatc aaactgataa gtatccaaga
     1861 tgtgtctgtg aagggatacg gagttgaatt gggttaagac cgcaatctta aaaattccgg
     1921 aattgatgca attactgatc gggatatcaa ttaaccggta ctgacctgcg atcgg
//
LOCUS       DAOMIV010000420         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_66002_length_1973_cov_6.233055, whole genome shotgun sequence.
ACCESSION   DAOMIV010000420 DAOMIV010000000
VERSION     DAOMIV010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66002_length_1973_cov_6.233055"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..>1973
                     /locus_tag="PKV59_08665"
     CDS             190..>1973
                     /locus_tag="PKV59_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HQP46859.1"
                     /translation="MAEKYNASNLSVDQILNFIKSGEIAIPDIQRPFVWKPRQVRDLI
                     DSLYNGYPTGYLIISQNPNLKLKDGTLSEGKKIMIDGQQRVTALLTAILGMEVIDADF
                     EKKKIKISFNPLANNEEEVFKVQDNAVLKDKKWIVDIAEVFKSDFKRGSFVNNYCELN
                     PEIDPDKLHDILDDLIGIKNRQIGIITLDKELTIDEVTEIFIRINSQGTKLNQADFAM
                     SKIAANEKFGGNILRKAIDYFSHLAVHPEWYQEMSKDKVFMESKYAPRIKWLKDDRDG
                     IYDPSYDDILRVSFMYKFGRAKMKDLVSLLGGRDFKTRDYKEEIAEDSFMRLSDCVMD
                     FMNEFSFFNFVLAIKSAGFVSSTLINSQMTLNFAYTLYLILNKNNQIDKTQIKRYVTK
                     WYVLSTLTSRYITSPETVMDMDISRIAEKGFVEYFKEIEEANLSDVFWKIGLVQNLET
                     SAINSPFFNVFLASQVRANDNSLFMNGTKVRDLITTMGDVHHIFPKGYLQKNGITDKT
                     KYNQIANYTYFDTQTNISVGEKPPYEYFRTVFIQCETKEPKIGNITDIEILKENLRVN
                     CIPENIVEMDFSHYEDFLLERRKMIAMK"
BASE COUNT          675 a          341 c          400 g          557 t
ORIGIN      
        1 aagtgctgcc tccggtatcg cgttttacac cgaccggaga ccgtacaaaa aaacgggaaa
       61 cagtactcga aaaactgacg gcttatttca aaaaattttg ggatatttcc ggcggaaaac
      121 tgttccgcta aatctatgat ttattgattt gcaattcgca ttaacactcg acgataatct
      181 tataatgtca tggcagaaaa atataatgca tcgaatcttt cggtagatca gattttgaat
      241 tttatcaaat cgggtgaaat agctattccc gatattcaac gaccctttgt ttggaaaccc
      301 agacaagtgc gggatctgat agactctctc tataatggat atccaacagg gtatctcatt
      361 atttctcaga atcccaacct aaaactaaaa gatgggacac tgtcggaagg caaaaaaata
      421 atgatcgacg ggcaacagcg tgtaacggct ctgttgacag ctatattggg tatggaagta
      481 attgatgcag atttcgaaaa gaaaaagatt aaaatttcat ttaatccatt ggctaacaat
      541 gaagaagagg tttttaaggt gcaggataat gctgtattga aagacaaaaa atggattgta
      601 gatattgccg aggtatttaa atccgatttc aaaagaggaa gttttgtcaa caactattgc
      661 gagctcaatc ctgaaataga tcccgataaa ttgcatgata ttttggacga tctaatagga
      721 ataaaaaata ggcaaattgg catcattacg ctggacaaag aactgacgat cgatgaagta
      781 accgaaatat ttatccgtat caacagtcag ggaacaaaac tgaatcaagc cgattttgcc
      841 atgtcgaaga ttgcggccaa tgagaaattt ggtgggaata ttttgcgaaa ggcaatcgac
      901 tatttttcgc atttggccgt ccaccccgag tggtatcagg agatgagcaa agacaaagtt
      961 tttatggaat cgaaatatgc acctcgaatc aaatggctga aagatgacag ggatggcatc
     1021 tacgatccaa gctacgacga tattttacgc gtttctttca tgtataaatt cggcagggca
     1081 aaaatgaaag atttagtgag tttgcttgga ggacgagatt ttaaaacacg cgattataag
     1141 gaggaaatcg ccgaagattc ttttatgaga ctgtcggatt gcgttatgga tttcatgaac
     1201 gaattttctt tcttcaactt tgttcttgcc attaaatcgg ccgggtttgt ttcaagtacg
     1261 ctgattaact ctcaaatgac gttgaatttt gcctatacgc tttatcttat actcaataag
     1321 aataatcaaa ttgataaaac ccaaataaaa cgttacgtta caaaatggta tgttctttct
     1381 accctgacaa gcagatacat cacctcccct gaaaccgtga tggatatgga tatctccaga
     1441 atagcggaaa aaggctttgt ggaatatttc aaagaaattg aagaagccaa cttatcggat
     1501 gtgttttgga aaatcgggtt ggttcagaat ttagaaactt ccgccataaa cagtcctttt
     1561 ttcaatgttt ttttggcatc ccaggtgcgg gccaacgaca attcgctttt catgaacgga
     1621 accaaagtaa gagatttgat aaccacaatg ggagatgttc atcatatttt tcccaaggga
     1681 tacttgcaga aaaacgggat aaccgataaa acaaaatata atcagattgc aaactatact
     1741 tattttgata cccaaacaaa catttcggtt ggcgaaaaac ctccgtacga atactttcgg
     1801 acggtattca ttcagtgcga aaccaaagaa ccgaaaatag gaaatataac cgatatcgaa
     1861 atcttaaaag agaatttaag ggtaaattgc attccggaaa atattgtaga aatggatttt
     1921 tctcattatg aagatttttt gctcgaacga agaaaaatga tagctatgaa gat
//
LOCUS       DAOMIV010000421         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_66078_length_1972_cov_3.051122, whole genome shotgun sequence.
ACCESSION   DAOMIV010000421 DAOMIV010000000
VERSION     DAOMIV010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66078_length_1972_cov_3.051122"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..1611)
                     /locus_tag="PKV59_08670"
     CDS             complement(349..1611)
                     /locus_tag="PKV59_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46860.1"
                     /translation="MKRMIIWLGLIGLFNFSIVYAQDSEQEDPPVWVPVYEEVFSPAS
                     EGKGWIMGTDTSETTVINRSMVQDGYLWEITSPKHQYSIVQNSSLVIPEGYQIKVEAA
                     VKLPYYEPPVCSGIVFGADSDDFDTFLVCNDQTYGFYHYSGTSWSESLPFTPVADFNQ
                     GEVLSLSSVIRNGWVDLYLDGKLLNTFPLNWTNRSIGLFAQPLSDKPTEILYKSLRIS
                     LAPQDSTASSLSEDVPSDIARVIRMLQLKGRIDTLGGDYRELADWDSSLAQMGYFQNH
                     PFDVTAQDFLIQSEISWKSAYEKPDTVDSGCGFTFRENSSESFLQAFVALDGNIYVRA
                     FRNGAEIPLVTYNYGQWSLEGNGIFTVVCSGSKISFLYNQQLLGTVTDATWISNGHIG
                     LTVFSGTNYDFGISCSFRKNQLYLFSEE"
     gene            complement(1629..>1972)
                     /locus_tag="PKV59_08675"
     CDS             complement(1629..>1972)
                     /locus_tag="PKV59_08675"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46861.1"
                     /translation="VRGDFDSFYKKELAYRREIGYPPFSRLVRIVISDPLQSVTEKRA
                     FDLSEEIRKILRTENHHATNLIGPAPCFFPKLDGKYRWHIILRGPNPTDIIKKVDTKT
                     VRVEVDPPSVL"
BASE COUNT          596 a          447 c          384 g          545 t
ORIGIN      
        1 gtgtccaagc cgagattgga aagatcaatc agggcatctc cctgccattg gcttaaatgt
       61 tttgcgcgcg gcgggaaata aagcggagtg caaacttcgg tttgacttcc taaaagtttc
      121 aatgttttct taaccgtttc cggccggtaa tatttcatca tggatccctc taataattaa
      181 tattcgaacc ttgactgcga tataaaaata tcgccctttc tattttatca atttatgcaa
      241 attttttcct attcttttta tatataatca caatgaatca gttcgcagaa aaatatattg
      301 aatcataaaa atattgcaga ttctcttatc agaaaaatca gctcattttt attcttcgga
      361 aaaaagataa agttgattct ttctgaatga acaagaaatc ccgaaatcat aattcgtacc
      421 ggagaaaaca gtcaatccga tatgaccgtt tgaaatccaa gtcgcatcgg taacggtacc
      481 gagcaattgt tgattataca agaatgagat tttcgaaccg ctgcatacca cggtaaatat
      541 gccattcccc tccagactcc attgaccgta attataagtg accaacggaa tttccgcacc
      601 gttgcgaaat gctctgacat agatatttcc atccaacgct acgaatgctt gtaagaatga
      661 ctcggatgaa ttctctcgaa aagtgaaacc gcagccggaa tcaacggtat ccggtttttc
      721 ataagccgac ttccaagaaa tctccgattg aatcagaaaa tcctgagcgg tcacatcgaa
      781 aggatgattt tgaaaatagc ccatttgagc aagtgaacta tcccaatcag ccaattctcg
      841 ataatcgccg cctaatgtgt cgattcgtcc ttttaattga agcatacgaa tcacacgggc
      901 gatgtcagag gggacatcct ccgaaagcga tgacgcagtc gaatcctggg gggctaatga
      961 aatcctcaac gacttgtaaa gaatctcggt cggcttatca cttagcggtt gcgcgaacag
     1021 cccgatgctc ctatttgtcc agtttaatgg aaaagtgttc aacaatttac cgtccagata
     1081 aaggtccacc cagccatttc gtatcaccga tgaaagagat agaacttctc cttggttgaa
     1141 atcggccacc ggtgtaaaag gcagagattc gctccaagaa gtgccggaat aatgatagaa
     1201 accgtatgtt tgatcattac aaaccaaaaa agtgtcaaaa tcgtccgaat ccgccccgaa
     1261 aacaattccg gaacaaaccg gcggctcgta gtacggaagc ttcacggcag cttcaacctt
     1321 gatctgataa ccttccggga tcaccaaaga tgaattttga acaatcgaat actgatgttt
     1381 tggagaggta atttcccaaa gatagccgtc ttgtaccata gaacggttga tcacggttgt
     1441 ttcggaagta tccgtaccca ttatccagcc ttttccttct gaagcgggac tgaatacttc
     1501 ttcatatacc ggtacccaaa ccggcggatc ctcttgttca ctgtcctgtg cataaacaat
     1561 cgaaaaatta aataacccga tgagtcccag ccaaataatc attcttttca taagtttcca
     1621 tgctttcttt ataatacgga cggaggatca acttcaaccc gaactgtttt tgtatccact
     1681 tttttaataa tatctgtggg attgggaccg cgtaaaataa tatgccaacg atatttccca
     1741 tccaatttcg gaaagaaaca cggtgcgggt ccgatcagat tggtcgcatg atggttttcg
     1801 gtacgaagaa tttttcgtat ttcttccgat aaatcaaagg ctctcttttc cgtaacggac
     1861 tgcagcggat cggatatcac aatccgtacc aatcgcgaaa agggagggta tccgatctcc
     1921 cgtcgataag ccaattcctt tttatagaaa gaatcgaaat ctccccgtac cg
//
LOCUS       DAOMIV010000422         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_66119_length_1971_cov_5.124739, whole genome shotgun sequence.
ACCESSION   DAOMIV010000422 DAOMIV010000000
VERSION     DAOMIV010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66119_length_1971_cov_5.124739"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PKV59_08680"
     CDS             complement(<1..267)
                     /locus_tag="PKV59_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HQP46862.1"
                     /translation="MDSHEYYEELILKDGDLSGEERKNLKAHLEECEKCQKLEEQWKS
                     CLNLLSKAPMVEPKADITGKWEQTLQVRTFEKEMKNQNRQIIGTI"
     gene            complement(273..833)
                     /locus_tag="PKV59_08685"
     CDS             complement(273..833)
                     /locus_tag="PKV59_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQP46863.1"
                     /translation="MNNISTDILEKVKEGDADAFTDIVDAYQNAIYNLCYRMLYNRTE
                     AEDATQETFWKAWTNFSKYDFNRSFSTWILSIAAHYCIDLQRKKKVPEIEIDETMEEI
                     IPDPIPHPEQEAIRNEEEQQIQDMLNSLNETDRAAIVLRYWYDFSDREIAESLSVSES
                     AVKSRLFRARKQLAQYWDGQTQFNQG"
     gene            complement(906..1958)
                     /gene="selD"
                     /locus_tag="PKV59_08690"
     CDS             complement(906..1958)
                     /gene="selD"
                     /locus_tag="PKV59_08690"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQP46864.1"
                     /translation="MSEEKKAIKLTELAFCAGCAAKLRAEVLMDVINPFGGLFNQNEF
                     PQLLIGLEEADDAAVYKLNDEQALVFTTDFFPPIVDNPYDYGAIAAANSISDIYAMGG
                     KPLMALNITDMPTNLPVEVIADIFRGGAEKAKEAGCPIVGGHTVRDQEPKYGLAVVGT
                     VHPDRILYKGKLKVGDKMVLTKPIGFGLMTTANKNGSADSEDFQKTVDWMKKLNKTAS
                     ELAVSVGCHSGTDVTGFSLMGHSWEMIRSGKIGLRYDWDAIPKIDHYEKYMNMWLFPG
                     GATDNRNYYGDKVTFTIALSEQQQMMLFDPQTSGGLLLGIGVDRLDEFLEKAKKSGQS
                     AWVIGEAVEKPGIEII"
BASE COUNT          499 a          508 c          348 g          616 t
ORIGIN      
        1 aatcgtcccg ataatttgtc gattttgatt tttcatttct ttttcgaagg tacgtacttg
       61 caatgtctgt tcccattttc cggtgatatc agccttcggt tcaaccatcg gggctttcga
      121 caacaggttc aaacatgatt tccattgttc ttccagtttt tggcattttt cacattcttc
      181 cagatgagct ttgagatttt tacgctcttc tccgctcaaa tctccatcct tcagaatcaa
      241 ttcttcataa tattcatgtg aatccatacc atttatccct gattaaattg agtctgtccg
      301 tcccaatatt gagctaactg ttttctggca cggaatagcc gacttttcac tgcactttca
      361 ctcacggaaa gactttcggc gatttcccga tccgaaaaat cataccaata tctcaataca
      421 atcgccgctc gatctgtttc atttaagcta ttaagcatat cttgaatctg ttgttcctcc
      481 tcattcctaa ttgcttcctg ctccggatga ggaatcggat ccggtatgat ctcttccatc
      541 gtctcatcga tttctatttc gggaactttc tttttccttt gcagatcaat gcaataatgc
      601 gcggctatcg aaagtatcca agttgaaaaa gagcgattaa aatcatattt tgaaaaattc
      661 gtccatgctt tccaaaaagt ttcctgtgtt gcatcctcag cttcggttcg gttatataac
      721 atccgatagc ataagttata aatcgcattt tgataggcat caacgatatc ggtaaaagcg
      781 tcagcatctc cttcttttac tttttctaat atgtcggtgg aaatgttgtt caaccttccg
      841 ctccaataaa ttctatctgt ctatacggaa aaaacgagaa agagttgcag cgcatgccaa
      901 caacgttaaa tgatctcaat tcccggcttt tccaccgctt cgccgatcac ccatgcactt
      961 tgccctgatt ttttggcttt ctccaaaaat tcgtcaaggc gatcgacacc gatgcccaaa
     1021 agcaaaccgc cgctggtttg cggatcaaat aacatcattt gttgttgctc ggaaagagcg
     1081 attgtaaaag tcactttatc tccgtaataa ttgcgattat ccgtagcccc gcccgggaat
     1141 aaccacatat tcatatactt ttcataatga tcaattttgg ggatagcatc ccaatcatat
     1201 cttaaaccga ttttacccga tcgaatcatc tcccaactat gccccattaa actgaatccg
     1261 gttacatcgg tacccgaatg gcagccgacc gacaccgcca attcggatgc cgttttattc
     1321 agctttttca tccaatccac cgttttttga aaatcctccg aatcagctga gccgttttta
     1381 ttcgcagttg tcatcaaccc gaacccgatc ggctttgtta ataccatttt atcgccgact
     1441 ttcaattttc ctttatacag aatccgatcc ggatgaaccg ttccaaccac cgccagtcca
     1501 tatttgggtt cctggtcgcg gacggtatga ccgccgacga tcggacagcc ggcttctttc
     1561 gctttttcag ccccgccgcg aaaaatatcc gcgatgactt ccaccggcag gttggtcggc
     1621 atatcggtaa tatttaatgc catcaacggc ttgccaccca tcgcataaat atccgatata
     1681 ctgttggcgg ctgcgatagc cccgtaatca tagggattat cgacaatcgg cgggaaaaaa
     1741 tccgtggtga ataccaatgc ctgttcatca ttcaatttgt aaacagccgc atcatcagct
     1801 tcttccaaac cgatcaacag ctggggaaat tcattctggt taaataagcc gccgaacggg
     1861 ttgatgacat ccatcaagac ttccgctcgc agttttgccg cacaacctgc gcaaaaggca
     1921 agttcggtta atttgatcgc ttttttctct tcgctcattt ttactccaat c
//
LOCUS       DAOMIV010000423         1970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_66170_length_1970_cov_4.684073, whole genome shotgun sequence.
ACCESSION   DAOMIV010000423 DAOMIV010000000
VERSION     DAOMIV010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66170_length_1970_cov_4.684073"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1509)
                     /gene="der"
                     /locus_tag="PKV59_08695"
     CDS             complement(145..1509)
                     /gene="der"
                     /locus_tag="PKV59_08695"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQP46865.1"
                     /translation="MKRPVVALVGRPNVGKSTLFNRLSGTRDAIVNDVPGTTRDRLFG
                     HAEWAGVEFDIVDTGGIDPSPSGQKAPLSVDSAEFVQEIKSQALLAVSEADVVLFLVD
                     AISGITPADREIASILRKNQKIVDGKETPPILLVANKADSARQRQNVSEFYELGMGDP
                     FPLSAIHGTETGDMLDALIALFPKQEEETPDDSVKIAIVGKPNVGKSSLLNRIVGEER
                     AIVSDIPGTTRDAIDTHILYNGVPVTLIDTAGIRRRGKIEPGVEKYSVIRSMRAIEEA
                     DVSLLVIDALSDITTQDAHIAGYILEEWKSVVVVVNKWDAVEKDTYTMQTYTDKIRKE
                     LNFMPYVPILFVSALTNQRVNQVIPTALQVQEERLARITTSQLNEVIHDAQDKHHATS
                     RTGRSLQIYYATQVRSDPPTFLIYVNDPKLAHFSYTRYLENQIRKQFGFIGTPIRIVY
                     RARR"
     gene            complement(1670..>1970)
                     /locus_tag="PKV59_08700"
     CDS             complement(1670..>1970)
                     /locus_tag="PKV59_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46866.1"
                     /translation="ETITLQWAGVGTLLDNEAYEVNIEDITGNTGRKLVDYVKDTKYI
                     VPMSFRPSDPSPHVIKWYVQPVRQSGSQADGTPIYTSAGARSEARTFIWSGVAVQ"
BASE COUNT          476 a          482 c          417 g          595 t
ORIGIN      
        1 tttccggttg tttttttgcc attcgggttt tatctctccg ctcggttgtt ttttctttta
       61 taatacacct gttttgtcag aacaatctaa ataagattct atttgttttt agagattatt
      121 ttcttttttc cggctcaaat agggctatcg tctggcgcga taaacaattc gaatcggtgt
      181 cccgataaac ccgaattgct ttcggatctg attctctaaa taacgggtat aactgaaatg
      241 tgctaatttc ggatcattca cgtagattaa gaatgtcggc ggatcactgc gcacttgtgt
      301 cgcataataa atctgcaacg atcgtcccgt tcttgaggtt gcatgatgtt tatcttgggc
      361 atcatggatg acttcattca attgagaagt cgtaatgcgg gctaagcgtt cttcctgaac
      421 ttgcagcgcc gtcggaatca cctgattgac ccgttgattg gtcagagcgg atacaaaaag
      481 aatgggcaca tatggcataa aattcaattc cttccgaatt ttgtccgtat aagtctgcat
      541 cgtataagta tccttctcga cggcatccca tttattgacc accacaacaa cactcttcca
      601 ttcttccaaa atatatccgg cgatatgggc atcttgagtg gtaatatccg acaacgcatc
      661 aatgacaagc aacgagacat cggcttcctc aatcgctcgc atggaacgaa tgaccgaata
      721 tttttcaact cccggttcaa tctttccccg gcgccgaatt ccggcggtat cgatgagcgt
      781 aaccggcaca ccgttataaa gaatgtgggt atcaatggca tcccgcgtgg ttcccggtat
      841 atcgctgacg attgcccgtt cttctcccac aattcgattc agcagactgg attttcctac
      901 gtttggtttg ccgacgatgg ctattttgac cgaatcatcc ggagtttctt cttcctgttt
      961 ggggaataaa gcaattaatg cgtccaacat atccccggtt tcggtaccgt ggatagcgga
     1021 aagcggaaat ggatctccca tcccaagttc ataaaattcc gatacgtttt gcctttgacg
     1081 tgcggaatcc gctttattgg caaccaataa aatcggaggc gtttctttcc cgtctactat
     1141 tttctgattc ttgcgcaaaa tcgacgcaat ttcacgatcg gcgggcgtaa tcccgctgat
     1201 tgcatcgacc aaaaaaagca ccacatcggc ttcgcttaca gccaaaagtg cctgagattt
     1261 tatttcctga acaaattcgg cggaatcgac cgaaagtgga gctttttgtc cggagggcga
     1321 aggatcaatt ccccccgtat cgacgatatc gaactcgaca ccggcccatt cggcgtgtcc
     1381 gaataagcga tcacgggtcg ttccgggcac atcattcaca atggcatctc tggtcccgga
     1441 tagacggttg aacaaagtac tctttccgac gttcggtctg ccgactaatg ccacaaccgg
     1501 tcttttcatt tcgttgattt tctcactttc cagtcctcaa taggatacta ttttaccatc
     1561 aaggtcgatc gtgacccggt tttgcattgg ttttcagaca ttttataatt ccatcaataa
     1621 aaacagcctt ttccaggctg tttttattga tcgtctttct attattattc tattggacgg
     1681 ctacgccgga ccaaataaat gttcgagctt cactgcgggc accggcggag gtataaatcg
     1741 gcgttccgtc cgcttggctg ccgctttgtc ttaccggttg aacgtaccat ttgatcacat
     1801 gcggagacgg atcgctgggc cggaaagaca ttggaacgat gtatttcgta tccttaacat
     1861 aatcgaccag tttacggccg gtatttccgg taatatcttc gatattgact tcatatgctt
     1921 cattatccaa caacgttccg actcccgccc attgcagagt aatcgtttca
//
LOCUS       DAOMIV010000424         1965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_66449_length_1965_cov_3.567539, whole genome shotgun sequence.
ACCESSION   DAOMIV010000424 DAOMIV010000000
VERSION     DAOMIV010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66449_length_1965_cov_3.567539"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..1636
                     /locus_tag="PKV59_08705"
     CDS             407..1636
                     /locus_tag="PKV59_08705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP46867.1"
                     /translation="MKIALFHYSAPPIIGGVESVIAHHARLMTANGHRVTIFAGRGET
                     FDPAIPVKVFPVLDSKNPEALTVKSELDKGLLSERFYSLCDQIQELLNNELPDFDVLI
                     AHNVTSLNKSLPLTVALFQNYRQPGFPRLIRWHHDLAWSSPRYRNELFDQYPWDLLRK
                     KWPDTTDVVVSQARRQELAQLMGLSAENISVIPNGVDLFSFFKFNEQTIRLIRTLNLS
                     EMDPLFLLPARLIPRKNIEFALQIMKAFIVNNPNAGLLITGPEDPHDPENALYRQNLM
                     RMRNEMGLQNTVHFLADVTDDFLPDEIISDFYRLADALLFPSFEEGFGIPLIEAGFEN
                     IPVFCSDIPVFHEIGGENLSYFDPNGDPSAVAKMIDDRLDSEMTFRWSRNAKNNYRWE
                     SIYRSLIEPLITEERKK"
     gene            1633..>1965
                     /locus_tag="PKV59_08710"
     CDS             1633..>1965
                     /locus_tag="PKV59_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46868.1"
                     /translation="MKTPFRKLKTVIVPIGRDGSGEAALSLAKAIANEVILVGVVSVH
                     NGESISAGTGAARSLRKRLLELSDSPKIRFKSTIIVSDSPWKDLMNVISDEKPQSVLI
                     EWKDDRLTE"
BASE COUNT          576 a          422 c          418 g          549 t
ORIGIN      
        1 acaaccgata atcctacacg gtctttcagt tatcgaattg tttcgaaaat tgatagactg
       61 cttgtaaaac ggatggcaaa aaatattttt ctgcgaaagc cgttgaaatc aaaacgattc
      121 gaaaactgtg gtataaaaaa taacagggat ttccatcgat taagaaatcc aaaaatttga
      181 ggagaatagt aaagggcaat atttcagcaa gaatttttta ttatccggat ccgaaccact
      241 cgatatgaaa ataggaattt ttttcagaca gacccggtcc gagtgattca aatacagaac
      301 aaacaaatca ttagaaaatt taaaaatcct tttcccgccc ttatactatt cattctgttt
      361 ttcgataaaa agacaggttt gagtaatatt ttggcggtaa attaatttga aaattgcatt
      421 attccattat tccgctcctc cgatcatagg cggtgtcgaa agcgtcattg ctcaccatgc
      481 tcggttgatg acggcgaacg gtcatcgggt gacgatattc gccggacgag gtgagacttt
      541 tgaccccgct attccggtaa aagtttttcc cgtgctggac tctaagaatc ctgaggcatt
      601 gaccgtaaaa tccgaattgg acaaaggttt gctttccgag cggttctatt ccttatgcga
      661 tcaaattcaa gagctgttaa acaacgaact tccggacttt gatgtcctga tagctcataa
      721 tgttacatcg ctgaataaaa gccttccatt aacggttgca ttgtttcaaa actatcgtca
      781 accgggattt ccccgtctca ttcgctggca ccacgatctg gcatggtcct caccccgcta
      841 ccggaatgaa ttatttgatc aatatccctg ggacctgctg cggaaaaaat ggccggatac
      901 caccgatgtc gtggtttcac aagcgcgcag acaagaatta gcgcaattga tgggattatc
      961 agcggaaaat atttcggtaa ttcccaatgg agtggatctg ttttccttct ttaaattcaa
     1021 cgagcaaaca attcgtttga tccggactct gaatctctcg gaaatggatc ctcttttcct
     1081 attgccggca cgcttaatcc ctcgaaaaaa tatcgaattc gctttgcaaa tcatgaaggc
     1141 atttatcgta aacaatccaa atgccgggct attgattacc ggtcccgaag atccccacga
     1201 tcccgaaaat gcgctttacc ggcaaaattt gatgcggatg cgaaatgaaa tggggcttca
     1261 aaataccgtt cattttcttg ccgatgttac cgatgatttt ttgccggatg aaatcatttc
     1321 cgatttctat cggttggctg atgcgttact tttcccgagt ttcgaagaag gtttcggcat
     1381 cccgttgatt gaagccggct tcgagaacat ccctgttttt tgttccgaca ttcccgtctt
     1441 tcatgagatc ggcggagaaa atttgagcta tttcgatccg aacggtgatc cgtccgctgt
     1501 cgcaaagatg atcgatgatc gtttggattc ggaaatgact tttcgctggt caaggaacgc
     1561 caaaaataat tacagatggg aatcaattta tcgttcgttg atcgaaccat taataaccga
     1621 ggagagaaaa aaatgaaaac tccttttcgc aaattaaaaa ccgtaattgt cccgatcgga
     1681 agagacggga gcggagaagc tgctctgtcg ctggcaaaag caatcgccaa cgaagtcatc
     1741 ctggtgggag tagtctccgt ccataatgga gaatccatca gcgccggaac gggagcggcc
     1801 cgatccttac gaaaaagatt gcttgagctc agtgacagtc ctaaaatacg ttttaaatca
     1861 acgataatcg tctctgattc accttggaaa gatttaatga atgttatctc agatgaaaaa
     1921 ccgcaatcgg ttttgatcga atggaaggat gacagactca ccgaa
//
LOCUS       DAOMIV010000425         1962 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_66592_length_1962_cov_6.570005, whole genome shotgun sequence.
ACCESSION   DAOMIV010000425 DAOMIV010000000
VERSION     DAOMIV010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66592_length_1962_cov_6.570005"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1053
                     /locus_tag="PKV59_08715"
     CDS             157..1053
                     /locus_tag="PKV59_08715"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQP46869.1"
                     /translation="MILVTGGTGFIGQALIRNLVSLGYPVRLLINPSGKSPRIPRGIA
                     VDVAISGIDDERNLRAAMKDVQIVFHLIGTEWKGIDAEYSTVDIPAAKTISAVAAQMG
                     VERFFYLSHLGADKASAYPVLQAKALCEAAVIASGVPYTILETAPVYGKGDHFTESFA
                     RLVRKVPFAVPLPGSGETKIQPIWIDDLITCLLLCLDDSKSIHRTFQIGGMEILSIRE
                     CLELIMEQIQVKKGFLNIPPVWLRGLLIAMEQWFEKAPKIYFWSDYLAEDRITALDVL
                     PREFSLIPTRMSRNLGYLTEAS"
     gene            1199..>1962
                     /locus_tag="PKV59_08720"
     CDS             1199..>1962
                     /locus_tag="PKV59_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46870.1"
                     /translation="MRKQAFSVHGHFYQPPRENPFTGKIPQEPGATPFHNWNEKILAQ
                     CYRPNAEDGNFGRISFDLGPTLATWMDENAPDVMKEIVRQERSFYEANGVGNGMALSY
                     NHTILPLATLDDKITQIKWGIADFEARFGHQPLGLWAPETAIDLETLMVMEQCGIRYT
                     ILAPWQANTRVGLDTSRPYWVDLPHEKRIAVFFYEEQTSMRVSFDPEATVNADRFMDQ
                     YLLPLYPDNSRRERMYLIASDGELYGHHQPLRNKFL"
BASE COUNT          527 a          434 c          491 g          510 t
ORIGIN      
        1 ttatgaccga ttgtttgttg aaataccatt gattgaaaat ccggaccgaa ccgcgacctc
       61 acaagagaag ccgaaaatgg atcccaaaag cgccgggtct tccgatccca aaaataaatc
      121 ggagcaatcc gacaacgcgg gcgattattt aaaaggatga ttctggtcac cggcggaacc
      181 ggtttcatcg ggcaggcttt gattcgaaat ctggtctcgt taggatatcc ggttcgctta
      241 ctgattaacc catccggcaa atctccgcgg attccgcgcg ggatcgcagt ggatgtcgcg
      301 atttccggta tcgatgatga acgaaacctg cgggcggcga tgaaagacgt ccaaattgtt
      361 tttcatttga tcggaaccga atggaaaggg atcgacgccg aatattcgac ggtggatatt
      421 ccggcggcga aaacaatttc cgcggtcgct gcgcaaatgg gggtcgagcg tttcttttat
      481 ctgagccatt tgggagcgga taaagcttcc gcttatcccg ttctgcaagc gaaggctcta
      541 tgcgaagcgg ctgtcattgc cagcggtgtt ccttatacga ttttagagac ggctccggta
      601 tacgggaaag gtgatcattt tacggaatct tttgcccgct tagtccgaaa agtaccattt
      661 gcggtcccgc tgcccggatc gggagaaaca aaaattcaac cgatttggat tgatgacttg
      721 atcacctgcc tgctgctttg tctggatgat tccaaatcga ttcatcgaac gtttcagatc
      781 ggcgggatgg aaatcctttc cattcgtgaa tgtttggaat taatcatgga acaaattcag
      841 gttaaaaaag gttttttgaa tattccgccc gtatggctgc gcgggcttct gattgcgatg
      901 gaacagtggt ttgaaaaggc accgaaaatc tatttttggt cggattatct cgcggaggat
      961 cggatcaccg ctttggatgt actgccgcgg gaattttctc taattccgac gcgtatgagc
     1021 cggaatctgg gctatctgac agaagcgtcc taagaaatat taataagcag taattatatc
     1081 gaataagtat ctaaataagg ataattataa gaattcggta aaaattaccg aaatattcca
     1141 atattttcca ttagcgagta taatacaaca atcttaataa agccagaaag gctgtttgat
     1201 gcgaaaacaa gcatttagtg ttcacggcca cttttatcaa ccgccgcgtg aaaatccctt
     1261 tacggggaaa ataccacagg aacccggagc aacccccttc cataattgga atgaaaaaat
     1321 tttagcacaa tgttaccgac cgaatgcgga agacggcaat ttcggaagga tcagttttga
     1381 tctcggtccg acacttgcca cttggatgga tgaaaatgcc ccagacgtga tgaaggagat
     1441 cgttcggcag gaacgatctt tttatgaagc gaacggagtg ggcaacggca tggcgctttc
     1501 ctataaccat accattctgc ccttggcgac tctcgacgat aagattactc agattaaatg
     1561 gggaatagcg gattttgaag cccgttttgg gcatcagccg ctgggtctat gggcaccgga
     1621 aacggctatc gatttggaga ccctgatggt aatggagcaa tgcgggatcc gctataccat
     1681 attggcgcct tggcaggcaa atacccgtgt cggtttggat acctcccgac cttattgggt
     1741 ggatctcccg cacgaaaagc gtatcgcggt tttcttttat gaagagcaga ccagtatgcg
     1801 tgtcagtttt gatcccgagg caacagtcaa tgcagatcga tttatggatc aatatttact
     1861 gccactttat cccgacaatt cgcgacggga acggatgtat cttatcgctt cggacggaga
     1921 actctatggg catcatcaac cgctgcgaaa taaatttctg ca
//
LOCUS       DAOMIV010000426         1959 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_66763_length_1959_cov_3.183824, whole genome shotgun sequence.
ACCESSION   DAOMIV010000426 DAOMIV010000000
VERSION     DAOMIV010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66763_length_1959_cov_3.183824"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="PKV59_08725"
     CDS             complement(<1..1431)
                     /locus_tag="PKV59_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HQP46871.1"
                     /translation="MYKIFPILFSCLIINASDAQSLKEKLFNSSRQCISLLNSLEKVN
                     NAGFRAFTMRFDTLSILFSDPDSPESLVSTMPVTYDASGRVKETKVINAGADDFFIYR
                     YHYIGSDNRFSTLDYFLHSGGTEDYAGVQIHLYDGHGRLISSKELDKNNNLIYGDSLT
                     IEYNATGQIEFYEYLFFQQGKWNLGRRVSNVLYNGPNLISYITTEVEKGHGDADTTVP
                     YLYDDVIFLNDDPSILFPDFDEFFEVDSLGVNDFFLSRDDAAPVYIKQPVKYIKYRTD
                     GSGTLVEMAEYASDDFLSRVNDVAGNEKYRVEYTFDGVGRLKKIHKSATGTVPEISTF
                     EYNELGRIARYEFTSDSLDFEVNSIYITDAKERLREYYQNLQDTHNSITNILRFGYKD
                     GQVSNNQQILEAVVFPNPASNTIHINLPQSSGRELPVKIFDTMGKMVYSGMTRADNRA
                     CTLTLDKNIPAGIYVLWINDGQITYHA"
BASE COUNT          564 a          370 c          415 g          610 t
ORIGIN      
        1 cgcgtgataa gtgatttgtc catcatttat ccaaagcaca tatataccgg caggaatgtt
       61 tttgtctaaa gttaaggtac atgctctgtt gtcggctctt gtcattccgg aatagaccat
      121 tttgcccata gtgtcgaaaa tctttacagg caattcgcgt ccggaagatt ggggaagatt
      181 gatatgaatt gtattgcttg cgggattggg gaatactacg gcttccaata tttgttggtt
      241 attgctgacc tgtccatcct tgtagccgaa tctaagaata ttagtgatgg agttatgagt
      301 atcctgcaga ttttgataat attcccgaag tcgttccttt gcatcagtta tatagatact
      361 gtttacttcg aaatcgagac tgtcggaagt aaactcgtat cgcgcgatcc ggcccaattc
      421 gttatattca aatgttgaaa tctcaggtac cgttcccgtt gcacttttat ggattttttt
      481 caacctgcct acaccgtcaa atgtatattc gaccctgtat ttttcatttc cagcaacatc
      541 atttaccctt gaaagaaaat catccgatgc gtattctgcc atttcgacca atgtacctga
      601 tccatccgtt cggtatttga tatatttgac cggctgtttg atgtacaccg gagcggcatc
      661 atccctgctc agaaaaaagt cattcacacc cagtgaatcg acctcaaaaa actcatcgaa
      721 atccggaaaa agaatggatg ggtcatcatt gagaaaaata acatcatcgt aaagataagg
      781 aacggtggta tctgcgtcgc catgcccttt ttccacttct gttgtgatat agctgataag
      841 atttggtccg ttgtaaagca cgttggagac ccttctgcca agattccatt tgccctgttg
      901 aaagaaaaga tattcataga attctatttg gccggttgca ttgtattcga tggtcaacga
      961 atcgccataa atcaaattat tattcttgtc caactccttt gacgaaatca gccttccgtg
     1021 accgtcatac aggtgaatct gcactccggc ataatcttcc gtcccaccgg aatgcaggaa
     1081 gtaatccaat gtggagaacc gattatcgct accaatgtaa tggtatctgt atataaagaa
     1141 atcatctgct ccggcattga tgacttttgt ttcctttact ctgccagagg cgtcgtaagt
     1201 taccggcatg gtcgaaacca gagattcggg cgagtcgggg tcggaaaaaa ggatggacaa
     1261 agtatcgaat ctcatcgtaa acgctctgaa tccggcatta ttgacttttt ccagtgaatt
     1321 taacaatgag atgcattgtc tggacgagtt gaataacttt tcttttaaag attgtgcatc
     1381 ggaagcgttg attatcagac aggaaaatag gataggaaat attttgtaca tcgtgatttt
     1441 gcttaaaaat gtcaccgaaa gtaaagataa tttatcgagc gtcggaaaat ttaacatagt
     1501 aacaaagtta tgatcgaccg tcaagggcaa taattcaggg ttgtgttttt tgtaatcatc
     1561 tctttttcat tgcttattgt aagggaaatt atttttcgga tcagacatcg gatcattaga
     1621 tattatggag aactattttc tctgttacta aatcggataa aggataattt ctttatgaat
     1681 cgaggggcga tgttgggtcg gatggcagga aaagcagtct ggtagagggt gattgcgaag
     1741 taggtaggaa aataatagcc ggaaattaag atatcttcgg tgtcgctaag atcagataat
     1801 acttaagtgg tcaattatcg gaaaatactt aagaactgat tttgcaaaag cccgccctga
     1861 ttgacttagg gattattaat catctaccta cttcgatatg aagctgtaga aacgaggctt
     1921 tgaccatatg ttgaaaattt tcgatttttc ccaaaatcg
//
LOCUS       DAOMIV010000427         1955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_66969_length_1955_cov_3.182632, whole genome shotgun sequence.
ACCESSION   DAOMIV010000427 DAOMIV010000000
VERSION     DAOMIV010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66969_length_1955_cov_3.182632"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="PKV59_08730"
     CDS             complement(<1..1165)
                     /locus_tag="PKV59_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQP46872.1"
                     /translation="MDFLNEIDLYLRAKATLMIVISAEEERILEELKTLCLNTGRSCV
                     SWDIADGFQSICGDLLPAGGRDPLTALEQIEKADARQPSLYILKDFNEFWENTTVKRK
                     LRTLAEQLKFTKKSIVITAASARIPVELLDESVILDYPLPNLDQLEEVLNKLQLTPGV
                     VLRLSEAGKEKLLQAALGLTASQARRVFAKSIVRHGYLSEEDIDFVSQEKKQIIREIE
                     ALEYTDSDESPENVGGLGELKKWLNSREKAFGSEAREYGLPAPKGIALIGIPGTGKSL
                     TAKMIGKLWHLPLLRLDVGALFGSLVGESEERVRRALELVELIAPCILWIDELEKALA
                     HGGLDSGTSTRVFGTILTWMQEKKSPVFVVATANDISTLPPELLRRGRFDEIFF"
     gene            1271..>1955
                     /locus_tag="PKV59_08735"
     CDS             1271..>1955
                     /locus_tag="PKV59_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46873.1"
                     /translation="MNNDLSQMPIIQIEGADLGYRTDAEPALKDITISFYPGDMVAVI
                     GPNGAGKSTMMKSIVGLIPPLKGKVNVHGELHGAHPDCVSYIPQRESIDWNYPITVRE
                     VVMMGRYGKIPFYKSPSEKDQKTVEDALKRMEITALADRKIRDCSGGQQQRVFLARSL
                     AQEPHILLMDEPFNAVDLATEKIILDNLRVFKERHVTTLVATHDLSLVSENFEKVLLL
                     NKRIIAYGNR"
BASE COUNT          507 a          470 c          473 g          505 t
ORIGIN      
        1 agaaaaagat ttcatcaaac cgcccgcgtc gcagcaattc cggcggaagg gtcgaaatat
       61 cattcgctgt cgcgaccaca aaaaccggtg acttcttttc ttgcatccaa gtcagaatgg
      121 taccgaaaac acgggtactg gttcccgaat ccaagccgcc gtgcgcgagc gctttttcca
      181 attcatcaat ccagagaata cagggcgcaa tcaattcgac caattctagc gctctgcgga
      241 cccgttcttc tgattcgccg accaggctac cgaataaagc tccgacatcc aaacgcagca
      301 acggtaaatg ccacagtttc ccgatcattt ttgcggtcag gctttttccg gtgcccggga
      361 ttccgatcaa tgcaatccct tttggagcgg gcaaaccgta ttcacgagct tccgaaccga
      421 acgctttttc gcgcgaattc agccattttt ttaattcacc caatccgccg acgttttcgg
      481 gagattcatc cgaatccgta tattccagcg cttcgatttc tcggatgatc tgttttttct
      541 cttggctgac aaaatcaatg tcttcctcgc tcagatagcc gtgccggacg atggatttgg
      601 caaaaacacg cctggcttgg gaggctgtca aacccaaagc cgcctgcagc agcttctctt
      661 ttccggcttc acttaatcgg agaaccaccc ccggtgtcaa ctgaagtttg ttcaacactt
      721 cttccaattg atcgaggttg ggtaagggat agtctaagat aacgctttca tccaacaatt
      781 ccaccggaat tctcgcagag gcggcggtga ttacaatgga tttcttcgta aatttaagtt
      841 gttccgcgag cgtccgtaat ttccgtttta cggttgtatt ttcccaaaat tcattgaaat
      901 ctttcaaaat atagagagaa ggttggcggg catcggcttt ttcgatttgt tccaaagcgg
      961 tcagcggatc tctgccgccg gccggcaaaa gatcgccgca gatggattga aacccgtccg
     1021 cgatatccca agacacgcag gatcgtcccg tattcaggca gagcgttttt aattcttcca
     1081 gaatccgttc ttcctcagcg gaaataacga tcattaatgt cgcttttgcg cgcagataaa
     1141 ggtcgatttc atttaaaaaa tccatgaaaa aaattatact atagggacga cagcgagata
     1201 aaaccgaccg tcatcgaagt gaagtgacca atccgataaa ggtgatagaa attcaagtac
     1261 aattcagcct atgaacaatg atctgagtca aatgcccatc attcagattg agggggctga
     1321 tctgggatac cggacggatg cggagccggc attgaaggat attacaatca gtttttaccc
     1381 gggtgatatg gtggctgtga tcggtccgaa cggtgccggg aaatcgacga tgatgaaatc
     1441 gatcgtcgga ttgatcccgc cgttgaaggg gaaggtcaac gtccacgggg aattgcatgg
     1501 cgcgcacccc gattgtgttt cctatattcc gcagcgcgag tcaatcgatt ggaattatcc
     1561 gatcacggtt cgggaagtcg tcatgatggg ccggtatggg aagattcctt tttacaaaag
     1621 cccttccgaa aaagatcaaa agacagtcga ggacgccttg aagcggatgg aaattaccgc
     1681 attggcggac cggaaaattc gggattgttc cggcggacaa cagcaacgcg tttttttggc
     1741 acgatcgctg gcgcaggaac ctcatattct gttgatggac gaaccgttta atgccgtgga
     1801 tttggcaacc gagaaaatta ttttggataa tttgcgtgtt tttaaagagc gccacgtgac
     1861 cacgttggtt gctactcatg atctttcgct cgtttcggaa aattttgaaa aagtgttgtt
     1921 gctgaataaa cggatcatcg cttacggaaa cagag
//
LOCUS       DAOMIV010000428         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_67007_length_1954_cov_3.945761, whole genome shotgun sequence.
ACCESSION   DAOMIV010000428 DAOMIV010000000
VERSION     DAOMIV010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67007_length_1954_cov_3.945761"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="PKV59_08740"
     CDS             complement(<1..939)
                     /locus_tag="PKV59_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQP46874.1"
                     /translation="MAGILIPFLIFTPWLTAILVWFIGDKHEEGLHQFASAGAILSAI
                     LSLLLLSQIGDSTISFAKIGPLFGNLSFSTDALGIILACIANCIGSLTVIFSAEYMKH
                     EKSLARYYSMVLLFIGAMSGLVLTANIFMIFFFWEITALCSYQLISFYNDDPNAVRGG
                     LKALFITQFGGIGLMVAGLLIFGYTHSFEIGRFIQAAQGGEIPANVLGIIGFTLIIAA
                     TAKSAQFPFFTWLPDAMEAPTPVSALIHAATMVNAGVYLLCRFFPALEAVPGWKMTIL
                     ILGLITILISSLMAIFATDLKRVLAYSTVSQLGYMFA"
     gene            complement(943..1242)
                     /locus_tag="PKV59_08745"
     CDS             complement(943..1242)
                     /locus_tag="PKV59_08745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HQP46875.1"
                     /translation="MNTLVIIAIVSLIAIGFYCLIITRNILKIIVALQVLVKGAMIAM
                     ILAGKLTDQMELAQSMAITIIVADTIVAVIGLAMSIQLREKTGTCDIHSISKLKR"
     gene            complement(1239..1739)
                     /locus_tag="PKV59_08750"
     CDS             complement(1239..1739)
                     /locus_tag="PKV59_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46876.1"
                     /translation="MPLVYFLVVAALIYSAHKATRVKGLLNAALWLAGTSCLTSILVY
                     ILGSPGLAVIELSVGAGLVTVLFVFAISIIGDEKITSDVFVPHWLAISISVVSFGMLA
                     YLLIAQHMPSAQSVTTSPIFSDLFWEGRKIDIYLQGALFISGILGILLLIAPAVSKRI
                     KEGKNK"
BASE COUNT          650 a          411 c          386 g          507 t
ORIGIN      
        1 ggcgaacata taacccagtt ggcttacagt cgaataagca agaacgcgct ttaaatctgt
       61 cgcaaagatt gccatcagag atgaaatcaa gatcgtgatc aaaccgagaa tcaggattgt
      121 cattttccaa ccggggactg cttccagagc gggaaagaaa cggcaaagga gatatacgcc
      181 tgcgttgacc atggttgcgg catgaatcaa agcgctgacg ggagtcggtg cttccatcgc
      241 atccggcagc caggtgaaga aaggaaattg agcgcttttc gccgttgcgg cgatgatcag
      301 agtgaaaccg ataatcccta aaacatttgc cggaatttct ccgccttgcg ctgcctgaat
      361 aaatcttccg atttcaaatg agtgtgtata accgaaaatc aacaatccgg caaccatcaa
      421 tccgatccct ccgaattgcg taatgaaaag agctttcaaa ccgccgcgta ccgcattggg
      481 gtcatcatta taaaaggaga tcaattgata ggagcataat gctgttattt cccagaagaa
      541 gaaaatcata aaaatattgg cggtcagtac caacccggac atagccccga tgaataacag
      601 taccatggaa taatatctcg caagactttt ctcatgcttc atatattctg cagaaaaaat
      661 cactgtcaac gaaccgatac agttggcgat acatgccaaa ataattccta aagcatctgt
      721 tgaaaaggag agattaccga aaaggggacc gatttttgca aaggatatgg ttgagtctcc
      781 gatctgagag agcaacagca aactcagaat tgcggaaagg atcgctccgg cacttgcaaa
      841 ttgatgcaat ccttcttcat gtttatcacc gataaaccaa accaatatgg cggtcagcca
      901 aggtgtgaaa ataagaaagg gaataagtat tccggccatt gtttatctct tcaatttcga
      961 aatggaatga atatcgcacg ttccggtctt ttcgcgtagt tgaatggaca ttgccaatcc
     1021 gattacggct acgattgtgt ccgcaacaat aattgtgatc gccatacttt gagccaattc
     1081 catttgatcg gttaattttc cggccaaaat catcgctatc atagctcctt taaccaagac
     1141 ttgaagtgct acgattattt tcaggatgtt tctggtaata ataaggcaat aaaaaccgat
     1201 tgcgatcaat gacacgattg caatgattac cagggtattc atttattctt cccctcctta
     1261 attcttttgg aaaccgcagg agcaatcagc aacaagattc cgagtatacc tgagatgaac
     1321 aaagcgccct gcagataaat atctattttt ctgccttccc agaaaagatc tgaaaaaatc
     1381 ggtgaggttg ttacagattg ggctgaaggc atatgctgag caatcaacaa ataagccagc
     1441 atgccaaatg atacaaccga tatggatatc gctaaccaat gaggaacaaa aacatccgat
     1501 gtaatttttt catcaccgat gatactgatc gcaaagacaa ataagaccgt taccagcccc
     1561 gctcccacac tcaattcgat tacggctaaa ccgggagatc ctagaatata aaccaatatt
     1621 gaagtcagac agcttgtccc ggctaaccat aatgcggcat ttaataatcc tttcactctg
     1681 gtagctttgt gagctgagta gattagtgct gcgacaacca gaaaatagac cagaggcatt
     1741 cgattcctcc catataggat aaaaagggaa aattaaaatt attaacttct gtccataatt
     1801 atatatccat ttttacgaaa ttcggaatat atttcgataa aatccgtttt ttcatcgaaa
     1861 taagcgaata aaaagatttt aattacctat tagagaaaaa aataacaaac aaagtttaat
     1921 atttatcgtc atagtaataa taaaaattcg tttt
//
LOCUS       DAOMIV010000429         1950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_67221_length_1950_cov_4.398417, whole genome shotgun sequence.
ACCESSION   DAOMIV010000429 DAOMIV010000000
VERSION     DAOMIV010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67221_length_1950_cov_4.398417"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PKV59_08755"
     CDS             complement(<1..610)
                     /locus_tag="PKV59_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46877.1"
                     /translation="MSQILLTIATFLAACIRMATPLTLAGLGEAFSERTGVMNLGVEA
                     IMLCGAFSSFIVTFWTGNVYWGMLCGMLAGMIISGIHAILCIKCKANQTIVGLALNFF
                     ALGLTSFLFLIVFGQSTILPQCKTVPNFEFPLLGKISLLGKAFFTHNIFVYLMYLLVF
                     IFLFIYKKTEWGVIFTAVGEHPSAADTAGINVFRIRYLACMIN"
     gene            complement(607..1644)
                     /locus_tag="PKV59_08760"
     CDS             complement(607..1644)
                     /locus_tag="PKV59_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46878.1"
                     /translation="MKNKAGDLISYLMIVLAGIILMILLIGITGNSISDALSGFIRGI
                     AGSSYSVAEVFVKATPLILTGLGVSVAFKSGFVNIGAEGQLYMGAVTITALALAFPNL
                     PKGILMPLCLLGGFLAGGVWSLIPGILKAKMGISEVINTIMFNYIALGIVGILLQTVL
                     KDPNNYFPVSATLTDSAGLSILIPGTRLHSGILIAVICAILIDILIFKTSLGFRLRAV
                     GMNPRACICSGISVGKHIIISSMVSGGLAGIAGVCEIAGLHHKLIEGISPGYGYLAII
                     VALLGGNNPFGVILAAIGIATLQVGSLSMQRAANVPSAISSIILGAIVLMLMARKTIF
                     KKLIDKKEEQG"
     gene            complement(1634..>1950)
                     /locus_tag="PKV59_08765"
     CDS             complement(1634..>1950)
                     /locus_tag="PKV59_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46879.1"
                     /translation="LILSRELCDEPKLIVACQPTRGLDIGATEFVREQLLMHRSKGAC
                     ILLISADLEEILSLSDRIAVLYKGRIMGILNNSKNIDITRIGLMMAGRNFNLNEVEIH
                     EK"
BASE COUNT          649 a          469 c          346 g          486 t
ORIGIN      
        1 cattaatcat acaagccaaa taccgaattc gaaacacatt gattcccgcc gtatcagcgg
       61 cactcggatg ttcgccgacc gctgtaaata taactcccca ttccgtcttt ttgtaaataa
      121 aaaggaaaat gaaaaccaac agatacatca ggtaaacgaa aatattatgt gtaaagaaag
      181 ctttaccgag caacgatatt ttgccgagca aaggaaattc aaaattagga accgttttac
      241 attgaggaag aatcgtgctt tgaccgaaaa cgattaaaaa cagaaagctg gtcagcccca
      301 gtgcaaaaaa attcaaagca agacccacaa ttgtctggtt tgctttgcat ttaatacaca
      361 gaatcgcatg aataccggaa ataatcatac cggcaagcat tccgcacaac ataccccaat
      421 aaacatttcc ggtccaaaaa gtcactataa aagagctaaa agctccgcac agcatgattg
      481 cttcgactcc caaattcatg acgccggtac gttctgaaaa agcctccccc aaacccgcca
      541 aagtgagagg tgttgccata cgaatacaag cggcaagaaa tgttgcgatg gttaataaaa
      601 tctggctcat ccttgctcct cttttttgtc tatgagcttc ttaaatatcg tttttcgcgc
      661 catcagcatc agaacaatcg ctcctaaaat aattgaggaa attgccgaag ggacatttgc
      721 cgcacgttgc atagaaagtg atccgacttg taacgtggca attcctatag ctgccaatat
      781 tacaccaaac ggattgtttc ctcccaacaa tgccacgata atcgctaaat aaccatatcc
      841 gggagaaatt ccctcaatca atttatgatg aagaccggcg atttcgcata cacccgctat
      901 cccggctaaa ccgccgctga ccatagatga aataataata tgtttaccca cagagattcc
      961 ggaacaaata caagcgcgcg gattcatacc caccgcccgc aatcgaaaac cgagtgatgt
     1021 tttgaaaatc aaaatatcga tcaggatcgc acagatgaca gctattaata ttcccgaatg
     1081 taatcgtgtt ccgggaatca aaatggataa accggccgaa tcggtcaagg tcgcggagac
     1141 aggaaaataa ttatttggat cttttagaac cgtttgcaat aaaattccga caattcccaa
     1201 agcgatatag ttaaacataa ttgtattaat gacttcggaa attcccattt tcgcttttaa
     1261 aataccgggt attaatgacc aaactcctcc ggctaaaaat ccacccaata aacagagcgg
     1321 catcaggatc cccttcggta aattgggaaa tgccaatgcc agagccgtga tcgttaccgc
     1381 tcccatataa agttgacctt cggcaccgat attcacaaag ccgctcttaa acgcaacgga
     1441 tactcccaaa ccggttaata tcaacggagt tgctttcacg aaaacttctg caacggaata
     1501 ggaagagcct gctatccccc gaataaaacc ggataacgca tccgaaatac tattgccggt
     1561 aatcccgatt aagaggatca taagaatgat ccctgccaga acaatcatca aataggatat
     1621 aagatcaccg gctttatttt tcatggattt caacctcatt caaattaaaa ttcctgcctg
     1681 ccatcattaa tccgatccgt gtgatatcaa tatttttact attattcaga atccccatta
     1741 ttctgccttt ataaagaact gcgatccgat cacttaacga aagaatttct tccaaatcgg
     1801 cagaaatgag taaaatacag gcacctttgc tacgatgcat taataattgt tctctgacaa
     1861 attccgtcgc cccgatatcg agacctcttg tcggttggca agccacaatt aatttcggct
     1921 cgtcgcaaag ctctctggaa agaatcaact
//
LOCUS       DAOMIV010000430         1945 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_67482_length_1945_cov_4.505291, whole genome shotgun sequence.
ACCESSION   DAOMIV010000430 DAOMIV010000000
VERSION     DAOMIV010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67482_length_1945_cov_4.505291"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1295)
                     /locus_tag="PKV59_08770"
     CDS             complement(144..1295)
                     /locus_tag="PKV59_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HQP46880.1"
                     /translation="MPNVEIIAIGSELLLGETQDTNTFYLLRKLRDLGFDVFRTTLIG
                     DNPKRIASTIQESLKRSDIVITTGGLGPTVDDPTRDAVAMAFGCKTEFKEELWMDIRN
                     YFDKMGRSVSENNIRQAYIPELAIAIPNPVGTAPAFYVYKDSKLVLSLPGVPAEMRYL
                     IEHSGTDLLLKYFPTQDSILVKTLHTFGLGESVMDEFVGDLEKSSNPTLGLSAKQGII
                     DLRITAKGKTKAEAERLLKDFENEVRNRLGNNIFGVDDETLPEVVNRMITRNYVKLNV
                     IEYGTGGAITKALSPSSIQTSSVQEMPDMSFSFRDHVQNIKSKMEIDSDSPLLICAIF
                     PISDSFIRSEIYLRTNRKEEYAERNYNSLTFSVDQTVLSAFEMIRKAII"
BASE COUNT          610 a          369 c          364 g          602 t
ORIGIN      
        1 aatgcgatcg gtaaagtccc accgacatct cctagaccac cgaccttgat taaaggatca
       61 gcttccgaaa tcagaaaaag gatatttaat ttctttgcca tgaatcattc cagttcatct
      121 tttgtttgga attaatttat tatctatatt atagccttac gaatcatttc aaaggcagat
      181 aaaaccgttt gatcaactga aaatgtcagt gaattatagt ttctttccgc atattcttct
      241 tttcgatttg ttcgaagata tatttccgaa cgaataaaag aatcagaaat cggaaaaatc
      301 gcacaaataa gtaacggtga atccgaatca atctccattt tgctttttat attctgtaca
      361 tgatctcgaa aagaaaagga catatccggc atttcttgaa cagaggaagt ttgtattgaa
      421 cttggtgata aggccttggt aattgcgcct cccgtgccat attcaatgac attcagtttt
      481 acgtaatttc gcgtaatcat acgatttacg acttcgggaa gggtttcatc atccacaccg
      541 aaaatgttat tccccaaacg gttacgcacc tcattttcaa aatcctttaa tagccgttcg
      601 gcttcggctt tcgtttttcc tttggctgtt attctcagat cgataattcc ttgtttggcg
      661 gataagccta aagtcggatt ggaagatttt tctaaatctc cgacaaattc gtccataacg
      721 gattcaccca agccaaaagt atggagtgtc ttaacgagaa tactatcctg tgtcggaaaa
      781 tatttaagca acaaatccgt tccgctatgc tcgatcaggt acctcatttc ggccggaaca
      841 cccggaagac tgagaacaag ctttgagtct ttataaacat agaaagccgg agcggtccct
      901 accggatttg gtattgcgat cgctaattcg ggaatataag cttgacggat attgttctcg
      961 gaaacagatc tgcccatttt atcaaaataa ttgcgtatat ccatccataa ttcttcttta
     1021 aattcagtct tacaaccaaa agccatagcg acagcgtcac gggtagggtc gtcgaccgta
     1081 gggccaagcc cgccggttgt aatcacaata tccgaccgtt ttaatgactc ttgaatcgtc
     1141 gatgcgattc ttttgggatt atcaccgatc agagtggtgc gaaatacatc aaacccgaga
     1201 tcacgcagtt tcctcaacag ataaaaagta tttgtatcct gtgtttcacc caataaaagt
     1261 tcggacccga tcgcaatgat ttctacattt ggcatattgc tcctttgaat cggagtggta
     1321 cttaaataaa tttttataaa aattatcgct gtcgaataga agttgcttga ttattaacag
     1381 cggtcttatt atagccttac cgacacgaaa caggctttta tgactcatga ttgatgatta
     1441 ttttgaagta gaatttcgag attcgaatac tctttacaag gtataatatt tttatccgga
     1501 aggattttta gatcatggtt tatctgtaaa aaattcggga gggaaagatg gagtcgaaaa
     1561 aagcggtttt caaaacatca gccggaggcg gtattaccga aaccatttaa caagtctatg
     1621 atgccatttt aaaacacgga tgtaattttg agggaaaagc ttgtacattc ggatcggatc
     1681 cggtcaaagc gaaagtcgga ttatttaaat gggcagaaac ggcagaagtc aacagcagcg
     1741 tcgatgtcct cggtgaaatt gtctccagaa ttgaataaaa atttcttatc caaaccatat
     1801 ttttcgaaat aatataaata tcctttcact gtttttataa ctgtgaaggg atattttgga
     1861 ttttaaaact attttgataa aaatctaccg atgatcattc tgaatgaata ataaaaagaa
     1921 aatttataat gaaaataaaa tatga
//
LOCUS       DAOMIV010000431         1942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_67651_length_1942_cov_3.965554, whole genome shotgun sequence.
ACCESSION   DAOMIV010000431 DAOMIV010000000
VERSION     DAOMIV010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67651_length_1942_cov_3.965554"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="mreC"
                     /locus_tag="PKV59_08775"
     CDS             complement(<1..546)
                     /gene="mreC"
                     /locus_tag="PKV59_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQP46881.1"
                     /translation="MIIFVTVIIVFTLIMIAYNLKYDTGKSFLRKIVLETVSPVYEVL
                     SGSIRSVQDAWLRYILLVGIQEENRNLKKKINELKAVLVSYQEGYQEAQRLKKLLAIT
                     DDDRYRFVTAKVIGREQAALSRTILINKGSSDGLKNGMPVIAYPGLIGRLTDVSWHVS
                     RVLLFIDENSNVDAIVQRNRTQ"
     gene            complement(776..1813)
                     /locus_tag="PKV59_08780"
     CDS             complement(776..1813)
                     /locus_tag="PKV59_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQP46882.1"
                     /translation="MFFDYVLGKFSNDLAIDLGTANTIVYVKDKGIVLNEPSVVAVHQ
                     DARGMKKVLAVGNEAKNMLGRTPGNIVAIRPMRDGVIADFDITEAMLKHFILSVHNRK
                     SLVRPRIIISVPSGITQVERRAVRETVESAGAREIYLIEEPMAAAIGAGLPVAEPTSS
                     MVVDIGGGTTEVAVISLAGIVYSKSVRVAGDKIDEAIAQYMKRKHNLLIGERTAEQIK
                     MEIGCAYPFDEIKVGSIKGRDLISGIPKITETNSEEIREAINEQVTFIVDAIKDTLEN
                     SPPELAGDIVDRGIVLTGGGALLANIDILIREETGLPITITDDPLSAVARGAGMALDQ
                     INVLKDIALEV"
BASE COUNT          532 a          495 c          363 g          552 t
ORIGIN      
        1 ctgggtccgg ttgcgctgga caatcgcgtc aacattactg ttttcatcaa taaataaaag
       61 cacacgggaa acatgccatg aaacatccgt cagacggccg atcaatcccg gataggcaat
      121 aaccggcatg ccgtttttca aaccatcgga cgaccctttg ttgatcaaaa ttgttctcga
      181 aagcgcggct tgttccctgc cgatgacttt cgccgtaaca aacctgtatc gatcatcatc
      241 cgtaatggcc agaagttttt tcagtctctg tgcttcctga taaccctcct gatatgaaac
      301 cagaacggct ttcaattcat tgatcttctt ttttagattc ctgttttcct cctgaattcc
      361 aacgagcaga atatagcgaa gccacgcatc ctgaacgctc cggatggaac cgctcagaac
      421 ttcgtatacc ggggaaactg tttctaaaac tatttttctc aaaaaacttt tacccgtgtc
      481 atatttcagg ttatacgcaa tcataatcag ggtgaaaaca atgataaccg taacaaagat
      541 aatcatttta tattttctta ataaaaacat tttttacctg acaaaaatta ttaagaagca
      601 ccgaatgcca acggtcagag tgaaagattg acaaatcttt tttcaaacat gttgcgataa
      661 atatgattca gtgaaaaatg ccttttgccg atttgctctc caccacaaag atccatcaaa
      721 aataaacgaa ccaccggaga ggaattcctg caaacagcat caagaccgga atggtttaaa
      781 cctcaagagc gatatcttta agcacgttta tttgatcaag agccatcccc gcgcctctgg
      841 cgaccgccga aagagggtca tccgtaatgg taatcggcag tccggtttct tcccggatca
      901 gtatgtcaat attggccagc agcgctccgc cgccggtcag gacaatacca cggtcaacaa
      961 tatcgccggc tagttccggg ggtgaatttt ccagagtatc tttaatcgcg tcaacaataa
     1021 atgttacctg ttcgttgatg gcctcccgga tttcttccga attggtttcc gttattttgg
     1081 gaatgcccga tattaaatcc cgtcctttta tggaacccac tttgatttca tcaaaaggat
     1141 acgcgcagcc tatttccatt tttatctgtt ccgctgttct ttctccgata agcaggttat
     1201 gttttctttt catgtattgg gcaatcgctt cgtctatctt atctccggca accctcaccg
     1261 attttgaata aacgattcca gccagggata taacggcgac ttccgttgtc ccgccaccga
     1321 tatccacaac catggaactg gtcggttcgg caacaggcaa cccggccccg atggcggcag
     1381 ccatcggttc ctcaatcagg taaatctccc gggctcctgc cgattcaacc gtctcgcgca
     1441 ccgcgcgtct ttccacctgc gtaatgcccg acggtacgga aataataatt ctcggccgca
     1501 ccagagattt gcgattatgg acactcagga taaaatgttt cagcatggcc tccgtaatat
     1561 caaaatcagc gatgacacca tcacgcatcg gcctgatcgc aacaatattt cccggggtgc
     1621 gtcccaacat gtttttcgct tcattaccga cggccagcac cttcttcatc cccctggcat
     1681 cctgatgaac ggccacaaca gagggttcgt tcaaaacaat tcctttatcc ttgacataga
     1741 caatcgtatt ggccgtacca agatcaatgg ccagatcgtt ggaaaatttc cctaatacgt
     1801 aatcaaaaaa catttatcct cctgtatttt cacaaattaa tataatgact ttcaggcttt
     1861 aatggttttt cataccgtat ttcattatcc taatcaactc atttttaaat tatatttgca
     1921 ttttgtaaat gactataata at
//
LOCUS       DAOMIV010000432         1939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_67805_length_1939_cov_3.628981, whole genome shotgun sequence.
ACCESSION   DAOMIV010000432 DAOMIV010000000
VERSION     DAOMIV010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67805_length_1939_cov_3.628981"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PKV59_08785"
     CDS             complement(<1..907)
                     /locus_tag="PKV59_08785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HQP46883.1"
                     /translation="MTETVQTEIRPVEKKKGSKLWLISLLLLVVLNFVVVKFYKPVEP
                     EINIATDSLLFHMEDGVAVRDPWFTIPFIGDVYLTNTMPAIALVFILLLLLAAAVHQQ
                     TKDGQLKSHGVVLLLEIIFSALTGMTDSAVEEKWRDRIYPFFLTVFMYVLIANLTKLL
                     PFFETFGLVYPTNENGFIAQKWGSWFYAIQSTAAEAGQQGYRLVSLFRGASTDLNFTV
                     AIAIVTVILIQVIGVKAHGFSYFHKFIDIKGMITNPKKGLINGFVGILELISEIAKIA
                     SFGFRLFGNMFAGMVLLLFLGYMVPW"
     gene            complement(900..1208)
                     /locus_tag="PKV59_08790"
     CDS             complement(900..1208)
                     /locus_tag="PKV59_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HQP46884.1"
                     /translation="MSIEKEIKKPKNIFGSDSRLLKKAFSLFLIIGIITLIIAFLSLW
                     GGRKLDSLYGTSPWIMIGLGIGGFLLVALIIVKMTKRVLRELNNSIKTSNKTGEDQND
                     "
     gene            complement(1300..>1939)
                     /locus_tag="PKV59_08795"
     CDS             complement(1300..>1939)
                     /locus_tag="PKV59_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46885.1"
                     /translation="LIALLFTLVWFMGFLWLKQANKEVTETHKTRAVTIPIELQDKSC
                     VLTIPACELELRTPEKISLANRANIDLKINPSENLYWDCNSEIPKNLRVQLFARIEFP
                     AAKIIPADQMGQEFPAGHEASFHWTIIPNQSGKNIQGQLWLKAEIMAVQTDSNQKLEE
                     WSLFSNEVKMTVIDIGKIPTDLLKRFLDGILLFGCGSWVIFILQKTFRKRRK"
BASE COUNT          593 a          440 c          347 g          559 t
ORIGIN      
        1 gccagggaac catatatccg agaaacaata aaagaaccat ccctgcaaac atatttccaa
       61 aaagccgaaa tccgaacgag gcgatttttg cgatttcgct gattaattcg agaattccta
      121 caaaaccgtt gatcaaaccc tttttcggat tggtaatcat cccttttata tcgataaatt
      181 tatggaaata gctgaatccg tgtgccttta caccgatcac ctgaatcaaa atgaccgtaa
      241 cgatggcaat ggccactgta aaatttaaat cagtagacgc accgcggaac aggctgacca
      301 aacgatatcc ctgctgtccg gcctctgcgg cggtagattg aatcgcataa aaccaacttc
      361 cccatttctg tgcgataaaa ccgttttcgt tcgtcggata gaccaatccg aatgtttcga
      421 aaaacggcaa taatttggtc aagtttgcga ttaatacata cataaatacg gtcaggaaaa
      481 aaggataaat tcgatctcgc cacttttctt ctacagcgct atccgtcatc cccgtcaacg
      541 ccgaaaaaat gatttcaaga agcaacacca cgccatgaga ttttaattgt ccgtctttcg
      601 tctgttgatg aaccgctgcc gccaacagaa gtaatagaat gaaaaccaga gcaattgccg
      661 gcattgtgtt tgtcaaatag acatccccga tgaaagggat cgtaaaccaa ggatctcgga
      721 ccgctacgcc atcttccata tgaaataata agctgtccgt cgctatgtta atttccggct
      781 caacgggttt atagaatttg acaacaacaa aattcagaac aaccaacagc aaaagggaaa
      841 taagccaaag ttttgatccc ttcttctttt caaccggacg aatctcagtt tgaaccgtct
      901 cagtcatttt gatcttcccc cgtcttattc gaagttttaa tagagttatt caattcccgc
      961 aaaactcttt ttgtcatttt tacaatgatc aaagcaacca ataaaaaacc accgattcca
     1021 agcccgatca tgatccaagg gctcgtccca tataaagaat ccagttttct tccaccccaa
     1081 aggcttaaaa aagcgatgat tagagtaata attccgataa ttaggaataa tgagaatgct
     1141 tttttgagaa gccgtgaatc actgccaaaa atattctttg gtttttttat ttccttctct
     1201 atgctcatac cgcacctttt cttactccta aatcaatcgt tattatatca gccatgcgat
     1261 ttcgtgattt acgtcacaga aagaattggt acaacgtact tattttcttc tttttcgaaa
     1321 agttttttgc aagataaata tcacccatga cccgcaaccg aatagtagaa ttccatccag
     1381 aaatctcttc aagagatcgg tcggaatttt cccgatatca atcaccgtca tttttacttc
     1441 gttagaaaac aaactccact cttccagttt ttgattcgaa tcggtttgta ccgccataat
     1501 ttcggctttc agccaaagct gcccttgaat attttttccg gattggttgg gaatgattgt
     1561 ccaatgaaaa gacgcctcat gaccggctgg gaattcctgt cccatctgat ctgcgggaat
     1621 gattttagca gccggaaatt caatccgtgc gaataattga actctgagat ttttaggaat
     1681 ttcggaatta caatcccaat acaaattttc agaaggattg attttcagat caatattcgc
     1741 tcgattagcg agggaaattt tttcgggggt tcggagctca agttcacacg cgggaattgt
     1801 aagaacacag gatttatctt gtaattcaat cggtatcgta acagcgcggg ttttatgagt
     1861 ctccgttact tctttattcg cttgcttcaa ccatagaaaa cccataaacc atacgagggt
     1921 aaacagcagc gcaatcaga
//
LOCUS       DAOMIV010000433         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_67911_length_1937_cov_3.486716, whole genome shotgun sequence.
ACCESSION   DAOMIV010000433 DAOMIV010000000
VERSION     DAOMIV010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67911_length_1937_cov_3.486716"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PKV59_08800"
     CDS             <1..636
                     /locus_tag="PKV59_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46886.1"
                     /translation="NTIFKFYYQVWILFAIASAYACSRIFEKLSSRKKWLIALPMFFV
                     TAACLIYPFQGITDNLEIVHRKVNETPSELTLDGSQFLKVSRPDDWAGIEWLKKAKPG
                     VIVEKVGASYTSDNAISTFSGHPTILGPTNHESQWRGGYKEIGTREADVQTIYESRNW
                     TTVKKLLQGYQVRYIFIGSAEQTAYKIQEQKFRDNLETVFQSGNCRIYQVY"
     gene            complement(667..1236)
                     /locus_tag="PKV59_08805"
     CDS             complement(667..1236)
                     /locus_tag="PKV59_08805"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQP46887.1"
                     /translation="MADLWTLFFMFFKIGITSFGGGYGMMPMIMDEGQRLVGLTEAQF
                     TDMAALDLITSGPVAVNSATYIGYIKGGYAGSLVATIASILPSILICIIVLIFLDRFY
                     NNKVVKGLFAGITPACGGLMFFTTATLAKSVYFNAETFTEVFNSTVTPTIIGMIVLTI
                     LTVIADIKFKVNPIYLTLAGAVLGIFFLA"
     gene            complement(1229..1849)
                     /locus_tag="PKV59_08810"
     CDS             complement(1229..1849)
                     /locus_tag="PKV59_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQP46888.1"
                     /translation="MQSDNIPVNLMTKKQKGFLIFKIFFSAMVFAFTGGSAATPTLIY
                     QVVKKYKLMSEDEYMEIVALSHSLPGIIGINNSMLSGYKVCGIFGSFMAVLGTALPAF
                     VSMLLVAVIFQELPQSRVVRGAINGIRAISIGILFDTGLRILIRNHKNIFGIAMIILA
                     MCLPLFTKISAFYTVLLSGLIGVVYYLTKNHNNLPAPIENKEEIDG"
BASE COUNT          597 a          421 c          401 g          518 t
ORIGIN      
        1 aatacgattt tcaagtttta ttatcaggtt tggatattgt tcgctatcgc ttccgcttac
       61 gcctgcagcc gaatttttga aaaattatct tcgaggaaaa aatggctgat cgctctccct
      121 atgttcttcg tcacagctgc ctgcttgatt tatcctttcc aaggaattac ggataatctg
      181 gaaatcgttc accggaaagt aaacgagact ccttcggagt taacattgga cggatcgcag
      241 tttctgaagg tctcacgacc ggatgattgg gcagggatcg agtggcttaa aaaagcgaaa
      301 cccggtgtga ttgtggaaaa agtaggtgcg agttatacct ctgataatgc cataagtact
      361 ttttccggtc atccgacaat tttaggtccc accaatcatg agagtcaatg gcgcggcggg
      421 tataaagaaa tcggaacccg ggaagcggat gttcaaacaa tatacgaatc aagaaattgg
      481 acaacggtaa aaaaactgtt gcagggctat caagttcgat atatttttat cggatcggcc
      541 gaacaaaccg catacaaaat acaggaacaa aaattccggg ataatttaga aacggtcttt
      601 caatccggga attgcagaat ttatcaggtt tattaaggtt ttcgtgttca agcgggcttg
      661 gacgaatcac gcgagaaaga aaattcctaa aacagctccg gcaagagtca gataaatagg
      721 attcactttg aatttaatat ccgctatgac agttaatatc gtcaaaacga tcatcccgat
      781 aatcgtcggt gtgacggttg aattgaacac ctccgtaaaa gtttccgcat tgaaatagac
      841 ggatttggcg agtgtggctg tcgtaaaaaa catcaacccg ccgcatgccg gtgtgattcc
      901 cgcgaaaaga ccttttacaa ccttattgtt ataaaacctg tccaggaaaa ttaggacaat
      961 aatacagatc aatattgacg gcaaaatcga ggcaatcgtc gcgaccagcg agccggcata
     1021 accgcctttg atatagccga tataagttgc ggaattgaca gccaccggtc cggaagtgat
     1081 taaatccaag gccgccatat cggtaaactg agcttcggtc agccccacca agcgttgtcc
     1141 ttcatccata atcatcggca tcatcccata tccgccgccg aaagaagtga tccctatttt
     1201 gaagaacata aagaaaagcg tccaaagatc agccatcgat ttcctcctta ttttcaatcg
     1261 gagccgggag attgttatga ttttttgtca ggtaatagac caccccgatc agcccgctca
     1321 gcagaaccgt ataaaaagca ctgatttttg taaataaagg caaacacatt gccagaataa
     1381 tcatggcgat tccgaaaatg ttcttatgat tccgaatcaa tattcgaaga ccggtatcaa
     1441 ataagatccc gatcgaaatg gctcggatac cgttaatcgc acctctgacg actctcgatt
     1501 gaggaagttc ttgaaagata accgccacta aaagcattga cacgaaggcc ggtaaagcgg
     1561 ttcccagaac cgccataaaa gatccgaata tgccacagac tttataaccg gataacatac
     1621 tgttgttgat tccgataatt ccgggcaacg aatggctgag tgcgacgatt tccatatatt
     1681 catcttcgct catcagttta tattttttaa caacctgata aattaaggtg ggggtagctg
     1741 cgcttcctcc cgtaaaagcg aataccatcg cgctgaaaaa aattttgaaa atcaggaatc
     1801 ccttttgctt ttttgtcatt aaatttacgg gaatattatc tgattgcatg aattaccccc
     1861 aatttctatc ttttggtcga accgattata acattccgaa cataattttc ttttttcaac
     1921 tccaattaca ggacata
//
LOCUS       DAOMIV010000434         1935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_68024_length_1935_cov_3.037234, whole genome shotgun sequence.
ACCESSION   DAOMIV010000434 DAOMIV010000000
VERSION     DAOMIV010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68024_length_1935_cov_3.037234"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PKV59_08815"
     CDS             <1..278
                     /locus_tag="PKV59_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46889.1"
                     /translation="PDDIFISHIMGMDTMALGLRMADKLIQDGRIDQFVEDRYASYKS
                     GIGKKIVDGTVTIQELEKYALEMGDVKTNKSGRQEYLEGIVNQILFG"
     gene            285..1808
                     /gene="xylB"
                     /locus_tag="PKV59_08820"
     CDS             285..1808
                     /gene="xylB"
                     /locus_tag="PKV59_08820"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HQP46890.1"
                     /translation="MSYLIGIDLGTSGTKTVLFDENGLVHGSETIEYPLYQEHNGWGE
                     QEPDDWWHAACDSIRSVIAQSKINPSDIKGLGISGQMHGLVMLDKDGKVLRRSIIWLD
                     QRTAKECEEITAKVGARRLIEITANPALTGFTASKILWVRNHQPEIYEKCAHILLPKD
                     YVRYKLTGEFATEVSDASGMQLLDVPQRKWSDEVLEKLDIDKNLLGTVYESQEITGVV
                     NARAATETGLKAGTPVVGGAGDNAAAAVGTGVVRDGLAFTTIGSSGVVFAHSSGIKID
                     PQGRVHTFCCAVPGAWHVMGVHQAAGLSLRWLRDNVCAEEVAEAEKRGIDPYDVMSEK
                     ADKITPGSDRLIYLPYLNGERTPHLDPNCRGVFFGLSGIHKRAHLIRAVMEGVSYSLR
                     DSISILREMDIETSEMTATGGGGRSFVWRQMLADNYHCPVKTIDNKEGPALGVAILAG
                     VGVGLFSSVPEACDHLIHDRSMQEPIQANSDVYDKYYPLYRKLYTDLKESFSELATL"
BASE COUNT          549 a          375 c          518 g          493 t
ORIGIN      
        1 agccggatga tatttttatc tcccatatta tgggcatgga tacgatggca ttggggttgc
       61 gaatggctga taagttaatt caggacggca gaatcgatca attcgtggaa gatcgttacg
      121 ccagttacaa gagtggcatc ggtaagaaaa ttgtcgacgg aacggtcaca attcaggaat
      181 tggaaaagta tgccttagaa atgggcgatg taaaaacaaa caagagcggc cgccaggaat
      241 atttggaagg cattgttaat caaattctgt ttggataaag ccatatgagt tatctgatcg
      301 gcattgatct tggaaccagt ggaacgaaga ctgttctatt tgatgagaac ggtctggttc
      361 atggttccga aaccatcgaa tacccacttt atcaggagca taacggttgg ggagaacagg
      421 aaccagatga ttggtggcac gctgcatgtg acagcattcg atctgttata gcgcaaagca
      481 aaataaatcc ttccgatatt aaaggcttgg gcatttcagg tcaaatgcat ggattggtca
      541 tgttggataa agacggcaaa gtactgcgtc gctccattat ttggctcgat caacggacag
      601 caaaggaatg tgaagaaatc actgcaaaag tgggggcgag acgtctcatc gagataaccg
      661 ctaacccggc actgacaggg tttactgcct caaagatatt atgggttcgg aatcatcagc
      721 cggaaattta tgaaaaatgt gcccatatcc ttcttccgaa agattatgtc cgttacaaac
      781 taaccggcga atttgccacc gaagtttcgg atgcctccgg aatgcagtta ttggatgttc
      841 ctcaacgaaa atggtccgat gaagttttgg aaaaattgga tattgataaa aatttactgg
      901 gaacagtcta tgaatcgcag gagatcaccg gtgttgtcaa tgcccgagcc gcaaccgaaa
      961 cagggttgaa agccggaaca cccgttgtcg gcggagcggg agataatgca gccgcagcgg
     1021 tcggaaccgg tgtcgtgcga gacgggttag catttacgac gatcggttct tctggtgtcg
     1081 tttttgccca cagttcagga atcaagatcg acccccaggg acgagttcat actttttgct
     1141 gcgcggtccc cggagcatgg catgtgatgg gggttcatca ggcagcgggg ctttcattga
     1201 gatggttacg cgataacgtc tgtgcggaag aagtcgcgga agctgaaaaa cgggggatcg
     1261 atccgtatga tgtgatgagt gaaaaggcag ataaaattac tcccggatcg gaccggctga
     1321 tttatttgcc ctatttgaac ggggaaagaa ccccgcattt ggatcccaat tgccgaggcg
     1381 tattcttcgg attatccggt attcataaac gggctcactt aatccgtgcg gtaatggaag
     1441 gtgtcagcta ttccttgcgg gatagtatca gtatcctgcg cgaaatggac atcgaaacca
     1501 gcgaaatgac cgcgaccggc ggaggcggca ggagctttgt ctggcgacag atgcttgccg
     1561 ataattacca ttgcccggtg aaaacaatcg ataacaagga aggtccggct ttaggtgtgg
     1621 cgatcctcgc cggagtcggt gtaggattgt tcagctcggt tccggaagcc tgtgatcatt
     1681 taattcatga tagatccatg caggaaccga ttcaagccaa ttcggatgtt tatgataagt
     1741 attatccgtt gtatcggaaa ctttatacgg atttgaaaga gtcattcagc gaattggcga
     1801 ctttatagga agggaaataa atctaaagta tttcgagaat aatgatagaa ttaatatcgt
     1861 ttatactttg gattttttcg aaaatcgttt tttatatcaa actggtaaat ccagcaaatt
     1921 aagaaggaga gatcg
//
LOCUS       DAOMIV010000435         1930 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_68259_length_1930_cov_4.433600, whole genome shotgun sequence.
ACCESSION   DAOMIV010000435 DAOMIV010000000
VERSION     DAOMIV010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68259_length_1930_cov_4.433600"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..760
                     /locus_tag="PKV59_08825"
     CDS             221..760
                     /locus_tag="PKV59_08825"
                     /inference="COORDINATES: protein motif:HMM:NF040142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46891.1"
                     /translation="MRQIFLTQTETILLDIVIWVIIHLSIGYWTSRIPVNVFNPESWL
                     FRTRKWEKDGMFYEKYFRVKTWKDHLPSGAALYEDAYEVKHIGDYSTENLRTWILESC
                     RSEFCHYLMIIPGFFFFLWNTVNVGWIMVAYAFANNIIPIIMQRYNRPRVKKLLRYME
                     RSKKYSRQQTVSSGEALAI"
     gene            complement(769..1488)
                     /gene="deoD"
                     /locus_tag="PKV59_08830"
     CDS             complement(769..1488)
                     /gene="deoD"
                     /locus_tag="PKV59_08830"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HQP46892.1"
                     /translation="MKKANLTPHIECSPEDFAKTVLLPGDPLRAKMIAETYLTDAELV
                     NHIRGANGYTGYYHGKRVSVMGSGMGMPSIGLYSYELYHFFNVSSIIRVGSAGAIAPD
                     LKLYDIILAQGACTDSNYFKSYHIPGKFAPIADFRLLRQAAEECEKNNISFQVGNILS
                     SDVFYREGGIAETLQWRKMGVLGVEMESAALYMNAARAGKKALTICTVSDLLESGEAA
                     SAEERQNAFNDMVKIALAIAE"
BASE COUNT          580 a          395 c          365 g          590 t
ORIGIN      
        1 ttcttgatgg tcacttttga gattatcttt tcggtataag atcaaaatgc tgcctgcaat
       61 taagccggat ttctaaatat cttgcgcttt tttttcgaga aaggatcatc cgattccatt
      121 aaccgaaaaa ttaatttggg cacttttcga aagaatatgg cacgttcatt caatgaaata
      181 attgaaagaa tcaaaatgtc gtgttaagat taatttaagt atgagacaga tatttctaac
      241 ccagaccgag acgattcttc ttgatattgt tatttgggta attattcatc tttcgatagg
      301 ttattggaca tctcgtatcc ccgtaaatgt ttttaatcct gaatcttggc ttttccgcac
      361 aagaaaatgg gaaaaagacg gcatgtttta tgagaaatat tttcgagtaa aaacttggaa
      421 agaccatctg ccttccggcg ctgcgcttta tgaagatgcc tatgaagtga agcatatcgg
      481 agattattcg accgaaaatc tgcgtacctg gatccttgaa agttgtcgtt cggaattctg
      541 tcactatctc atgatcatcc ccggcttttt cttttttctt tggaataccg ttaatgtcgg
      601 atggattatg gtagcttatg catttgcgaa caatataatt cccattatta tgcagcgcta
      661 caatcgtccg cgggtgaaga aacttttacg atatatggaa cgttctaaaa aatattcccg
      721 acaacaaacc gtcagcagcg gggaagccct cgccatataa atttttcgtt attctgcgat
      781 ggctaaagca attttcacca tgtcattaaa tgcattttga cgttcttcgg cacttgccgc
      841 ctcaccgctt tcaagcaaat ccgatacggt gcagatagtt aaagccttct tgccggcgcg
      901 tgccgcattc atatataagg ctgccgattc catttccaca cctaagacac ccatctttcg
      961 ccattggaga gtctccgcaa tgccgccttc ccggtaaaaa acatcagagg ataggatatt
     1021 tccgacttga aatgaaatat tatttttttc acattcttcg gcagcttgtc gtaataatcg
     1081 gaaatccgca atcggagcaa atttgcccgg gatatggtag cttttaaaat aattcgaatc
     1141 cgtacaagcg ccttgtgcaa gaatgatatc gtacagtttt aaatccggcg caatggcacc
     1201 tgccgatccg acccgaataa tggaactgac gttgaaaaaa tgataaagct cataggaata
     1261 gagtcctatg gaaggcattc ccatcccgct gcccataacc gaaacacgct tcccatggta
     1321 ataacccgta tatccgtttg ctccgcggat atggtttacc aattccgcat cggttaaata
     1381 agtctctgca atcatttttg cccgcagagg atcaccgggt aacaaaacgg tttttgcaaa
     1441 atcctcggga ctgcattcaa tatggggtgt aagattagct tttttcattg aacctccggc
     1501 gtcgaaatga gagtaaactc actggtataa tcatagtgaa aattataatt tagctgacaa
     1561 agtttcaaat agatcgcggc aatttaagtt cctgttttct gagaactcac ggctaaaaag
     1621 caaagaacag aatttttcac ttgagaccga ttttaccaaa taaattcaca ttgatagata
     1681 ctgattctcg gaacgaatct cctccgtttc ttttatgact aatcgaaata tctgatttca
     1741 aatctttatt tcagctaaaa catgtattta ataaggataa aatttttatg aatattttaa
     1801 tttttaattg tggcagttca tcacaggcat tcaaagttta ccaagtctcg gaaaatgatc
     1861 agccgaaaat gatcgcatcc ggaaaagcaa aaaatgttgc tacgcaaacg aaagcggatt
     1921 cattcataga
//
LOCUS       DAOMIV010000436         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_68610_length_1924_cov_4.205457, whole genome shotgun sequence.
ACCESSION   DAOMIV010000436 DAOMIV010000000
VERSION     DAOMIV010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68610_length_1924_cov_4.205457"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1169)
                     /gene="dnaJ"
                     /locus_tag="PKV59_08835"
     CDS             complement(105..1169)
                     /gene="dnaJ"
                     /locus_tag="PKV59_08835"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQP46893.1"
                     /translation="MTKKDYYEILNVSRTASDEEIKKSYRAIAMQCHPDRNPGDKKAE
                     EQFKEAAEAYEVLSDREKREIYDHYGHEGLNSTGFRGFSGFEDIFSHFSNIFDDVFGY
                     GNARGHSRASGMAGADLRYDLKISFLDAAFGLTTTIELEKLATCKQCNGTGAAPGSSP
                     ETCRTCKGRGQVIQSSGFFTISSTCPHCNGYGKFISKPCNNCHGTGKQPITKKVQLKI
                     PAGVETGSRLRLRGEGEAGSKGGPDGDLYVFIHVEEHEYFSRSGDDIICRVLVSFVNA
                     TLGGTIEVPTLQGKEKLKIPRGTQNGKIFRLKGKGIPHIRGFGHGDQIIEVSVEIPTS
                     LSKKQEELLIEFEKLNSLNK"
     gene            complement(1284..>1924)
                     /locus_tag="PKV59_08840"
     CDS             complement(1284..>1924)
                     /locus_tag="PKV59_08840"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HQP46894.1"
                     /translation="AEYPAECSPYRESLEGRSMLVKKATPLPVECVVRGYLSGSGWKD
                     YQKSKTVSGIKLPDGLKDSSKLPEPIFTPSTKAPEGEHDAPINGKEMEDLIGAELTNK
                     VIKISLSVYKRAAKTSLKAGIIIADTKMEFGLIGNELILIDELLTPDSSRFWPADDYA
                     EGRAQKSFDKQFLRDYLLSIKWDQKPPAPKLPAEIIENTRKKYEEALKRLVG"
BASE COUNT          460 a          464 c          409 g          591 t
ORIGIN      
        1 tttcatatat cttcctccca tgatttgttt tttattttaa agcatatatt gccgatgatt
       61 gcaattgcta ttatggcaaa tccgcaattt atgaatggga aaaattattt attgagcgaa
      121 ttgagttttt cgaattcgat gagaagctct tcctgctttt tactcagcga tgttggaatt
      181 tccacggaca cttcaatgat ttgatcaccg tgaccaaaac ctctgatatg agggatgcct
      241 ttccccttca gtctgaatat ttttccgttc tgtgtgcctc tgggaatctt caacttctcc
      301 tttccttgga gagtcgggac ttcaatggtt ccccccagag ttgcgttgac aaaagaaacc
      361 agaacacggc aaatgatatc gtccccggat ctgctgaaat attcatgttc ttcaacatga
      421 atgaacacat ataaatcgcc gtccggtcct cccttgcttc cggcttcacc ttcgccgcgc
      481 agacgaagac gcgagcctgt ttccactccg gcagggattt tcaattgtac tttttttgtg
      541 atcggctgct tgccggtacc gtggcaatta ttgcatggct tggagatgaa ttttccgtaa
      601 ccgttacagt gcggacacgt ggaactgatg gtaaagaagc cgctggattg aatgacctgt
      661 cccctgcctt tgcaggtgcg gcatgtttcc ggcgaagatc cgggagccgc tccggtgccg
      721 ttacattgct tgcaagtggc taatttttcc aattcaatcg tggtggttaa tccgaaagcg
      781 gcatccagaa aggaaatttt caagtcataa cgcagatcag cgccggccat accggatgca
      841 cgcgaatgac ctcttgcgtt accataaccg aaaacatcat caaaaatgtt gctgaaatgg
      901 gaaaaaatat cttcgaagcc gctgaaaccc cggaaaccgg tgctgtttaa cccttcatga
      961 ccgtaatgat catatatttc tctcttttcc ctgtcgctta aaacctcata cgcttctgcc
     1021 gcttccttga attgttcttc agctttttta tcacccggat ttctatccgg atgacactgc
     1081 atcgctatcg cccggtaact tttttttatt tcttcatctg aagctgttcg ggaaacattc
     1141 aaaatttcgt aataatcttt cttcgtcata cgcttttcgg gtcaacctct ttgtgcattt
     1201 taaaaacggg aaataacgat ctgcacggta atctaacttt aatttaataa taaatcatca
     1261 ttcgtcaata tgaagggtat aagctacccc acaagacgtt tcagcgcttc ctcgtatttc
     1321 ttcctggtat tttctataat ttccgcggga agttttggcg ccggcggctt ctgatcccac
     1381 ttgatagaaa gcagataatc gcgcaggaac tgcttgtcaa aacttttctg cgcgcggcct
     1441 tccgcgtaat cgtcggcagg ccagaaacgg gacgagtcgg gcgtcagcaa ttcgtctatg
     1501 agaattaatt cattgccgat gaggccgaat tccatctttg tatcagcgat aatgattccc
     1561 gccttcagag aggtcttagc cgctctttta taaaccgaca aggaaatttt tataactttg
     1621 ttggtaagtt ccgcaccaat gagatcttcc atttcttttc cgttgatagg agcgtcatgt
     1681 tcaccttccg gcgctttggt gctgggtgta aaaatcggtt cgggtaattt ggatgaatct
     1741 ttcagcccgt ccggtaattt gatgccggaa acagtcttgc ttttctgata atctttccag
     1801 ccggagccac tcagatagcc tcgaacaaca cactccaccg gcaggggtgt ggctttttta
     1861 accagcatgc ttctgccctc cagactttca cggtagggag agcattctgc cggatattca
     1921 gctg
//
LOCUS       DAOMIV010000437         1909 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_69485_length_1909_cov_4.117044, whole genome shotgun sequence.
ACCESSION   DAOMIV010000437 DAOMIV010000000
VERSION     DAOMIV010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69485_length_1909_cov_4.117044"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PKV59_08845"
     CDS             <1..516
                     /locus_tag="PKV59_08845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQP46895.1"
                     /translation="PGTWLIVPGGSREFTNWSAPFITRENPAVASIYGPGACQSVMDG
                     PVGSGVFIWPTTETRISGYNYSEETNHRAIDIAGSTGNAVYASDAGVVVYAGWNDWGY
                     GNVVMIDHGNGWQTLYAHLDSYNVQCGYYVAAGDVIGAVGTTGNSSGPHLHFEMRYNG
                     IPQNPNNFFNK"
     gene            528..1703
                     /locus_tag="PKV59_08850"
     CDS             528..1703
                     /locus_tag="PKV59_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQP46896.1"
                     /translation="MEEKNHRWSNPIRYMALVGLILFAGAVVYLSHETLNLIIAVAIV
                     AYILSPVVNFFVRKLHMGRGFAVVLAYLILIILIIILLVLIVPVITGRIQGFLSTDWP
                     TVINSIDSWLEGMIRQLEVQKIQIGNVGVDLSVPLIELRGKLNSINVEDFNISSIIPD
                     ISSAWQSIISVSANLISRILGGLLTIVTSIMISIHLCRDGWKLKDWIVKQFPENYQIE
                     TDEFLNRLGNIWNSYFVGELKLMLIIGIVTFGVCTALGLHWALPLGIVAGFLEVIPNI
                     GPIISCIPAIFSAAIFGSYWLPLDNFLMVLVVIIAYILIQQIENIIVVPRVMSHALDI
                     HPVLIIIGILILNKRIGLFGALLAAPILGLLKVTLHFVTCKIRNEDPYPELYNPPSA"
BASE COUNT          472 a          400 c          465 g          572 t
ORIGIN      
        1 cccggaacat ggctgatcgt acccggagga tcccgtgaat tcacaaactg gtccgctccg
       61 tttattactc gggaaaatcc ggcggtagct tccatctatg gaccgggagc ctgtcagtct
      121 gtcatggacg gaccggtcgg cagcggtgta tttatctggc cgacgactga aacccgcata
      181 tcgggatata actattccga agaaaccaat catcgggcaa tcgatattgc cggttcgacc
      241 ggtaacgcgg tttatgcttc cgatgcgggc gtggtggtat atgccggttg gaatgattgg
      301 ggatacggaa atgttgttat gatcgatcat gggaacggat ggcagacttt gtatgcacat
      361 ttagattctt ataatgtaca atgcggttac tatgtggccg caggggatgt aatcggtgcc
      421 gtaggaacaa ccggtaactc atccggaccg catttgcatt ttgagatgcg atataacggc
      481 attcctcaaa acccgaataa cttttttaat aaatgacgga acaaaagatg gaagaaaaaa
      541 atcaccggtg gagtaatccg attcgataca tggctttggt cggcttgatt ctgtttgcgg
      601 gcgccgtcgt ttatctttcc catgaaactt tgaatctgat tattgcggtg gcgattgttg
      661 cgtatatcct gtcgccggtg gtaaatttct ttgtccgaaa attgcacatg ggaagagggt
      721 ttgcggtcgt actggcatac ctgattctga ttattcttat catcatttta ttggttctga
      781 tcgtcccggt tatcaccggc cggattcaag ggttcctttc caccgactgg ccgacagtta
      841 tcaatagtat cgattcctgg ttggaaggaa tgattcggca attggaagtc caaaaaatac
      901 agatcgggaa tgtcggagtg gatttatccg tcccgttgat cgagctgcgc ggaaagctga
      961 attccattaa tgtggaagat tttaatattt catcgatcat tccggacata tcttcggctt
     1021 ggcagtctat catatctgtt tccgcaaatt taatctccag aattctcggc gggcttttga
     1081 ccatcgttac ttctattatg atttccatcc atttatgcag ggacggttgg aaactgaaag
     1141 actggatcgt taagcaattt ccggagaatt atcagatcga aaccgatgaa ttccttaatc
     1201 ggttaggaaa tatttggaac agctattttg tgggtgaact gaaattgatg ctgattatcg
     1261 gtatcgtgac tttcggtgta tgcacggcat tggggctaca ttgggctttg ccgttaggga
     1321 tcgtagccgg atttttggaa gtcattccga atatcggtcc cattatctcc tgtattccgg
     1381 caattttttc agctgcgatt ttcggatctt attggctgcc gctggataat tttctgatgg
     1441 tgttggtcgt gattatcgct tatattctga ttcaacagat agaaaacatc atcgtcgttc
     1501 cgcgagtaat gagccatgcc ttggatatcc atccggttct gatcattatc ggcatattga
     1561 ttctaaataa aagaataggt ttattcggcg ctttgttggc ggcgcctatt ctcgggctgc
     1621 tgaaagtgac tttgcatttc gtgacttgta aaattcgaaa cgaagaccct tatccggagt
     1681 tatacaatcc tccgtccgcg tgatatagaa ttttaaaata aaagggctca ccgaaagaag
     1741 agcccttttt gtaaacaatt tatgatgaaa atgattaccc taaccgggct ctttcctcat
     1801 cgacgatcga tgtgtccaat ttcttaaaca gcggacaaac ctcaccgaat ttttgaccga
     1861 ccggcaattc ggccggttgc caagaaccga tcgcctcgga agcgtcgta
//
LOCUS       DAOMIV010000438         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_69547_length_1908_cov_4.370750, whole genome shotgun sequence.
ACCESSION   DAOMIV010000438 DAOMIV010000000
VERSION     DAOMIV010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69547_length_1908_cov_4.370750"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PKV59_08855"
     CDS             <1..232
                     /locus_tag="PKV59_08855"
                     /inference="COORDINATES: protein motif:HMM:NF037014.2"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-diphosphate and nicotinate from nicotinate
                     D-ribonucleotide and diphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HQP46897.1"
                     /translation="TNFHTKKLLVPIYEKGKLVYKLPTLQQIRRNCQKNVDELWEEIR
                     RLDNPHTYYVDLSQKLWDLKTQMIKEARMISQ"
     gene            277..1419
                     /locus_tag="PKV59_08860"
     CDS             277..1419
                     /locus_tag="PKV59_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP46898.1"
                     /translation="MDKIRTAVIGCGKVGATHALAYKTLPNSELCAVCDVSQKNANQF
                     AKDYNVRAYSDIKKMIRENGVQVVSVCTPHPLHAEPTCEAAEAGANVICEKPLASDLR
                     DCDRAITACEKAGVKLGVISQRRFYWPVIRMIQAIRDGKIGKPIIGTIEVMGWRSPEY
                     YQMDGWRGKWREEGGGVMVNQTPHQIDLLQWMMGDIDELFGYWDNFNHPEIEVEDTAL
                     AVIRFKNGGIGQILVSNSQKPGLWGRIHIHGSNGASVGAQVEGGSSFISGVTTSKVEP
                     AFNDIWTVPGEENMPEVWKKEDQIKSEGIDPMTYFHQIQIADFLDAVIHNRPPSVDGH
                     EGRKTVEIFTAVYRSQRDHRPIKFPLSAEDGSDFDGRLSYVPYSHR"
     gene            1438..>1908
                     /locus_tag="PKV59_08865"
     CDS             1438..>1908
                     /locus_tag="PKV59_08865"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HQP46899.1"
                     /translation="MKALVLTDYKKMEIQDVPVPIIAEDEVLIQVKACGICGSDIHGY
                     DGSSGRRQPPVIMGHEASGIIVETGAKVLGWKKGDRVTFDSTIYCGHCSYCREGLINL
                     CENRRVLGVSCDEYRRDGAFAEYIAVPQHILYALPDEVDFNQASLVEPLSVAVHA"
BASE COUNT          546 a          389 c          492 g          481 t
ORIGIN      
        1 tacaaacttt catacaaaga aacttctggt cccgatttac gagaaaggga aattggttta
       61 taagttaccg acgctccaac agattcgaag aaattgtcag aaaaatgtcg atgaattatg
      121 ggaagaaatt cgtcggttgg ataatccgca tacctattat gttgatcttt cacaaaagtt
      181 gtgggatttg aaaacccaaa tgataaaaga agcccgtatg atttctcaat aatttatgca
      241 ggtgaaactt aatttggata acggaaggga atgtaaatgg ataaaattcg aacggctgtg
      301 atcggatgcg gaaaagtggg agccactcat gcgcttgctt ataaaacgct tccaaattcc
      361 gagctctgtg cggtatgcga tgtctcacaa aagaatgcga atcagtttgc gaaagactac
      421 aatgttcggg catattccga tataaaaaaa atgattcgtg agaatggggt ccaagttgtt
      481 tcggtctgta ctccgcatcc tttgcatgcc gaaccgacct gcgaagcggc ggaagcgggt
      541 gcgaacgtga tctgtgaaaa accgttggcc tccgatcttc gggactgtga tcgggcaatt
      601 accgcatgcg aaaaagccgg tgtgaaattg ggagttatca gccagcgtcg tttttattgg
      661 ccggttatac gcatgattca agccatccgg gacggaaaaa tcggcaaacc aatcatcggg
      721 acaatagaag ttatgggttg gcgcagcccc gaatattacc agatggatgg ttggcgggga
      781 aaatggcgcg aagaaggcgg cggtgtaatg gtcaatcaaa cccctcatca gattgattta
      841 ttgcaatgga tgatgggtga catcgatgaa ttattcggct attgggataa tttcaaccac
      901 ccggaaatcg aagtggaaga tacggcgtta gccgtaatta gattcaaaaa cggcggaatt
      961 ggtcaaatac tcgtttcgaa ttctcaaaaa ccgggcttat ggggcagaat tcacatccat
     1021 ggttcaaacg gcgcttccgt gggagcacaa gtagagggag gttcttcttt tatttcaggc
     1081 gtaaccacct caaaagtgga accggctttt aatgatattt ggactgtccc gggggaagag
     1141 aacatgccgg aagtctggaa gaaagaggat caaatcaaat ccgaagggat cgatccgatg
     1201 acttatttcc atcagatcca aatcgccgat tttctggacg cagtcattca taatcgaccg
     1261 ccctccgttg acggacatga aggtcgaaaa acggttgaga tattcaccgc cgtttatcgt
     1321 tcccaacggg atcaccggcc gataaagttc ccgctctccg ccgaagatgg atctgatttt
     1381 gacggtcgct taagttacgt cccttattca caccgttgaa aggaaaagaa gaaaaaaatg
     1441 aaagccttag ttctgaccga ttataaaaaa atggaaattc aagatgtacc ggtgccgatc
     1501 atcgcagaag atgaagtgtt gatacaggta aaagcctgcg gaatttgcgg ttccgatatt
     1561 cacggatatg acggttcatc aggcaggcgg cagccgccgg tgattatggg gcatgaagca
     1621 agcggaatca ttgttgaaac cggagcaaaa gtccttgggt ggaaaaaagg ggatcgcgtt
     1681 acctttgatt ccacgattta ttgcggtcat tgttcctact gccgggaagg tctgattaat
     1741 ctgtgcgaaa atcgtcgtgt tttaggcgtg tcgtgtgacg aatatcgtcg ggatggtgcg
     1801 ttcgccgaat atattgccgt tcctcagcat atcctctatg cgttaccgga tgaagtcgat
     1861 tttaatcaag cctccctcgt agaaccgctt tccgtcgccg tgcatgcg
//
LOCUS       DAOMIV010000439         1908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_69555_length_1908_cov_3.650297, whole genome shotgun sequence.
ACCESSION   DAOMIV010000439 DAOMIV010000000
VERSION     DAOMIV010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69555_length_1908_cov_3.650297"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PKV59_08870"
     CDS             complement(<1..1205)
                     /locus_tag="PKV59_08870"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQP46900.1"
                     /translation="MSDQIKKVVLAYSGGLDTSVIIPWLIDTYHCEVICACADVGQEE
                     ELNGLEERAKASGASKLYVKDLTDELCENYIWPMIKSGAVYEKKYYLGTSIARPIVAK
                     WLVEIAEKEGADAVAHGCTGKGNDQVRFENTVAALNPNLKVIAPWRIWDFTSREDLIK
                     YLDSKHIPYPDSAKNPKLFSRDRNIWHISHEGGPIENIETEPDESFWVLTVSPEKAPD
                     KPEYVEIEFDAGAPVAVNGERMKPKDIVKTLNKIGGKNGIGRCDMVENRLVGMKSHGL
                     YETPGGTILNYAHSELESITLDRDSQHYKEVVASRYAELIYNGQWFSPLCSAMNKFFE
                     EIDKTTSGTVKLKLYKGNIIPAGRISKYSLYSEKMASFGDVSLYDHKDATGFIRCFTL
                     PNRVRAMME"
     gene            complement(1496..>1908)
                     /locus_tag="PKV59_08875"
     CDS             complement(1496..>1908)
                     /locus_tag="PKV59_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46901.1"
                     /translation="RSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQE
                     MLTVKSDDIQGRVSTYEAIVKGKPIQEPGLPASFRVLVKELQSLGLAVEALLDTGETV
                     RFGKEEERMKTPHMESGLLDAGKETPINLSDLLN"
BASE COUNT          481 a          465 c          398 g          564 t
ORIGIN      
        1 tgttccatca ttgcgcgaac cctgttggga agtgtaaagc aacgaatgaa tccggtggca
       61 tctttatgat cataaagtga gacatcgccg aaagacgcca ttttttcgct gtagagactg
      121 tatttcgata tccttccagc cggaatgatg ttgcccttat acagcttcag cttgactgta
      181 ccacttgttg ttttatcaat ctcttcgaaa aatttattca ttgcgctgca taggggggaa
      241 aaccattgtc cgttataaat cagttccgca taacgggagg cgaccacttc tttataatgc
      301 tgagaatcac gatccagagt gatggattct aattcggaat gagcatagtt tagaattgtc
      361 cccccgggtg tctcataaag cccgtgagat ttcattccga ccaaacggtt ttcaaccata
      421 tcgcagcggc cgataccatt tttcccgccg attttgttta gtgtctttac aatatccttc
      481 ggtttcattc tttcgccgtt gacggcaacc ggagcgccgg catcaaactc gatttctacg
      541 tattccggtt tgtccggtgc tttttccggt gagacggtca acacccaaaa cgattcatcc
      601 ggctccgttt cgatattttc gatcggtccg ccttcatgcg agatatgcca aatattccga
      661 tcccggctga agagcttcgg attttttgcg gaatccggat aagggatatg tttgctgtcg
      721 agatatttaa tcagatcttc ccgtgaggta aaatcccaaa tccgccaagg tgcgattact
      781 ttcaggtttg gattcagcgc agccacggta ttctcgaagc gtacctgatc atttccttta
      841 ccggtgcagc catgagcaac ggcgtcggca ccttcttttt cggcaatttc gaccaaccat
      901 tttgcgacaa tcgggcgggc tatagaagtc ccgaggtagt attttttctc ataaacagcg
      961 cccgacttga tcatcggcca aatgtagttt tcgcaaagtt catccgtgag atctttgaca
     1021 tataatttgg aagccccgct ggcttttgcc cgctcttcca gtccgtttaa ttcttcttcc
     1081 tgtcccacat ccgcacaagc gcaaataact tcgcaatgat aggtatcaat caaccaaggg
     1141 attattacgg aagtatctaa accgcctgaa taagctaaaa cgactttttt gatttgatct
     1201 gacatattgt ttctcctgaa cgaaataaat ttaatcaaaa aaaagacctt tgccgattca
     1261 gcagaggtct tttgtctatc cgatcgtatg atttaaccga aactttccgc tgaatacatt
     1321 cctgtaatgc ggcagagtcg tcggatatag aaaaaaaccg gttgctgact tttcatgacc
     1381 gttattataa atgaatccaa agtcggaaaa agacaaattt ataaaaatag tgattatttc
     1441 aaaaaagtcc atcgagattt ctttcgatgg actttcgaat tgttttctga ttaacttagt
     1501 tcagtaagtc ggacaaatta atcggcgttt cttttccggc atccagtaaa ccgctttcca
     1561 tatgaggagt cttcatccgt tcttcttcct tgccgaatcg gaccgtttca cctgtatcga
     1621 gaagggcttc aaccgccaag cccaagctct gcaactcttt gaccagaaca cggaaggaag
     1681 ccggcaaccc gggttcctga atcggtttgc ctttaacaat ggcttcatag gtgctgacac
     1741 gtccctgtat atcatcagac ttgaccgtca acatttcctg cagcgtatat gccgcaccat
     1801 aggcttccag tgcccagact tccatttcac cgaaacgttg tccgccgaat tgagcttttc
     1861 ctcccagcgg ttgctgcgtc accaaactgt atgggcctgt ggaacggg
//
LOCUS       DAOMIV010000440         1904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_69777_length_1904_cov_3.445646, whole genome shotgun sequence.
ACCESSION   DAOMIV010000440 DAOMIV010000000
VERSION     DAOMIV010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69777_length_1904_cov_3.445646"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..>1904
                     /locus_tag="PKV59_08880"
     CDS             97..>1904
                     /locus_tag="PKV59_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQP46902.1"
                     /translation="MTENGGAESRFSKDTAYLRGVIFCITVLITFALLTFQISVRKTS
                     YSLSVGDVVNQDIVAPRTITFESQILTEQARNDAQNMVDKIYLPADPTISRNQVQYLR
                     LSLQFISAIRSDQYATEAQKIEDIQKLIFVRLDEPTIKQILELDDSEWKLVQSECQRI
                     LEQVMQNSIRDDQVNSVIENIPTMISYSINPKTADLINSITRRFVVPNSLYSAELTEK
                     KRQEARDAVEPRERTFIANQTVVSRGQIITELIYEALDKMGLVHSKNDNRKILSSGLL
                     VLGLTVFALYFFLIDHRHHQLNLRECILIASLYLTFLFAARLLIPNRTILPFIFPIQT
                     LGLTISCLSGQAFGIVMSIIIGLLVPYDFPNALGYASFYIISSLTGILVLRKARQVTD
                     FLLAGLAAGSIGIPIIIAFQVAESPMDMIGVVTLAAASVGSGLLSTGFTLIFQYSFSK
                     FIGVASSLELLELIRPDSPLLQYILNTAPGTYQHSLMVANLSEQAAKEIDADPLLVRA
                     GSMYHDAGKALNPSFFIENQVNGSINLHEDISPKESAASIIQHVNDGVMLVEKYRLPE
                     VIKDFVREHHGTNVARYQYTEAVNQEGEEAIDINDFT"
BASE COUNT          546 a          410 c          400 g          548 t
ORIGIN      
        1 tatgggaaaa gttatgaaaa ccttgttgcc aaaagtgaaa ggtttatgtt ctaatgagaa
       61 agtcagtaag gcagtaaagg attatttagc aaacctatga cggaaaacgg aggagcagaa
      121 agcaggtttt caaaggacac agcctactta agaggagtga tcttttgtat taccgtttta
      181 atcacctttg cgctcctcac ttttcagatt tctgtcagaa aaacaagtta cagcctgagt
      241 gtcggagatg tcgtcaatca ggatatcgtc gcaccgcgga ccattacttt tgagagccaa
      301 attctcaccg aacaagcgag aaatgatgct caaaatatgg tggataaaat ctatcttcct
      361 gccgacccga cgatttctag gaatcaggtt caatatctcc gtttatcgct gcaattcata
      421 tctgcaatcc gcagcgatca atacgctacc gaagctcaaa aaattgagga tatccagaaa
      481 ctgatattcg ttcgtctgga tgaaccgaca attaagcaga tcctggaatt ggatgactcc
      541 gaatggaaat tggtacaatc tgaatgccaa cggattttag agcaggtcat gcagaattcc
      601 attcgtgatg atcaagtcaa ttcggtaatt gaaaacatcc cgacaatgat cagctattcg
      661 atcaacccta aaaccgccga tttaattaac agtattactc ggcgatttgt ggtaccaaac
      721 agcctttaca gtgcggaatt aaccgagaaa aaacgacagg aagcaaggga tgccgtcgaa
      781 ccgcgggaaa ggacttttat tgcaaatcag acggttgtat ccagaggtca gataattacc
      841 gaattaatct atgaagcatt agataaaatg gggctggtgc actctaaaaa tgataatcga
      901 aagattcttt cgtctgggct gcttgttctc ggtttaaccg tctttgctct ctattttttc
      961 ctgatcgatc atcgtcatca tcaattaaac ctacgcgagt gcatcttaat tgcgagtctc
     1021 tatttaacat ttctttttgc tgccaggtta ctcataccca atcggacaat tctgccgttc
     1081 atttttccga ttcaaacatt agggcttaca atttcttgtt taagcggaca agccttcgga
     1141 attgtgatgt ccattattat cggattgctc gtgccttatg actttcccaa cgctctcgga
     1201 tacgcttctt tttatatcat ttcatctttg accggtattt tagtccttcg aaaagcacga
     1261 caagtgacgg attttctgct tgcaggttta gctgccggct cgatcggaat cccaattatc
     1321 atcgcttttc aggttgccga gtctccgatg gatatgatcg gggttgtcac tctggcagct
     1381 gcatcggtag gcagcggttt gttgtccacc ggttttacgc ttatttttca atattccttc
     1441 tcaaaattta tcggggttgc ctcatcactt gaacttttag agctgattcg tccggattcc
     1501 ccgttgctgc aatacatctt aaataccgct cccggaacat atcaacattc attgatggtc
     1561 gcaaatcttt ctgagcaagc cgcaaaagag attgatgccg atcctcttct tgtacgtgcc
     1621 ggatcgatgt atcatgatgc cgggaaagca ttaaatccgt cttttttcat tgaaaatcag
     1681 gtaaacggat cgatcaactt acatgaagat atttcaccga aagaaagcgc ggcatccatc
     1741 atccaacatg tcaatgacgg agtgatgttg gtcgaaaaat atcgattacc cgaagtcatt
     1801 aaagattttg tccgcgaaca tcacgggacg aatgtcgcga gatatcaata caccgaagcg
     1861 gtaaaccagg aaggggaaga agcgatcgac ataaatgact ttac
//
LOCUS       DAOMIV010000441         1897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_70176_length_1897_cov_3.425081, whole genome shotgun sequence.
ACCESSION   DAOMIV010000441 DAOMIV010000000
VERSION     DAOMIV010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70176_length_1897_cov_3.425081"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1897
                     /locus_tag="PKV59_08885"
     CDS             <1..>1897
                     /locus_tag="PKV59_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46903.1"
                     /translation="QTTSEIYQKYAKIAEAIQAAGSDHIWTEEDEWTKEQQDAYWNMV
                     KEGWMNEVQKIIDYAYTNFAADYAQEILGKTPEEVGSDEGLKTTFGMAMWGFGDIADG
                     ILTTKSGAEFDLNAGNKPTIDDFYKETFAAYAGDADAFFSVEATDVKAKSIVATAKDT
                     FISEEGSKEPEMAAGIPNISGIKKVDDYTVEVKTYGYEAPAIYSILGIYVAPLHYYGD
                     PAQYDYENNRFGHPYGDLSIIEAKTSNPMGSGPYKFIKYDNRIVYFEANENFYLGKPK
                     TQFVQFKETADNEMVEGVSTGTIDAASISGSKSTFESIAEHNSNKEITGDKITTFKVD
                     NLGYGYIGINADTVKVGDDPASTESKNLRKALATVIAVYRDVAYDSYYGDAATVINYP
                     ISNTSWAAPQPTDPDYKVAFSEDVNGNPIYTSGMTPEEKYEAALEAAKGFLKAAGYTY
                     DEATGKFTAAPEGASLTYEIIIPAGGTGDHPSFALVTDAANALAKIGITLKINDPADT
                     NVLWDTLDAGKQNLWCAAWQATIDPDMYQIYHSSNIVGKGGSDSNHYHIADPELDKAI
                     IEARQSDDQAYRKEIYKTALDIIVDWAVEIPAYQRQNSTIFSTERVKIDTVTPDITTF
                     YDWNAEIQ"
BASE COUNT          597 a          407 c          444 g          449 t
ORIGIN      
        1 ccaaacgacc agtgaaatct atcagaaata cgcaaaaatt gccgaagcca ttcaagccgc
       61 cggctctgat catatttgga ccgaagagga cgaatggacc aaagagcagc aggacgctta
      121 ttggaatatg gtcaaagaag gctggatgaa cgaagtacaa aaaattatcg attatgctta
      181 taccaatttt gcggcggact atgcacaaga aatcttaggc aaaacacccg aggaagtcgg
      241 cagcgatgag ggtttaaaaa ccactttcgg tatggcaatg tggggattcg gcgatatagc
      301 agacggcatt ttaacaacga aatccggtgc ggaatttgac ttaaatgcgg ggaataaacc
      361 gacaatcgat gatttctaca aggaaacctt tgcagcttat gcgggagatg ccgatgcatt
      421 cttctctgtt gaagcgaccg atgtaaaagc caaatcgatt gtggcaaccg ctaaagatac
      481 attcatttcg gaagaaggtt caaaagaacc ggaaatggct gcggggattc cgaacatctc
      541 cggaattaag aaggtggacg attatacggt cgaagtaaaa acctacggat atgaagcccc
      601 ggctatttat tccattttgg gaatttatgt cgctccgtta cattattacg gagatccggc
      661 acaatatgat tatgaaaata atcgcttcgg acacccttat ggggacttaa gtatcatcga
      721 agcaaagacc tctaatccga tgggttcggg accgtacaag tttatcaaat atgataatcg
      781 aatcgtatat ttcgaagcca acgaaaattt ttatcttgga aaaccgaaaa ctcaattcgt
      841 tcaattcaaa gaaaccgccg ataatgagat ggtcgaaggc gtcagcacgg gcacaattga
      901 tgccgcctcg atctcgggat caaaatccac atttgaatca atcgccgaac ataacagcaa
      961 taaagagatt acgggagaca aaatcacgac tttcaaagtt gacaatctcg gctacggcta
     1021 tatcggaatc aatgcggata ccgtaaaagt cggggatgac ccggcttcaa ccgaatcaaa
     1081 gaatttacgg aaagcactgg caacggtaat tgccgtatat cgtgacgtag cttatgacag
     1141 ttattatggt gatgcggcaa ccgttattaa ttacccgatt tcaaatacct cgtgggcagc
     1201 acctcagcca acggatcccg attataaagt ggcattctcc gaagatgtaa acggtaaccc
     1261 gatttacacg tccggcatga ctccggagga gaaatatgaa gcagcccttg aagcggcaaa
     1321 aggctttctt aaagcagccg gttatactta cgatgaagcc accggaaaat ttacggcggc
     1381 tccggaaggt gccagcctga cctatgaaat tatcattccg gcaggcggga caggggacca
     1441 cccctctttt gcacttgtta cggatgccgc caatgccttg gcaaaaatcg ggatcacgtt
     1501 gaagattaac gatcccgctg atacaaatgt tttatgggat acattggatg ccggtaaaca
     1561 aaacttatgg tgcgcagctt ggcaggctac aattgatccc gatatgtatc agatttatca
     1621 cagttcaaat attgtcggta aaggcggttc ggattccaac cactatcaca ttgccgatcc
     1681 cgagttggat aaagcgatta tagaggctcg ccaaagcgat gaccaagctt atcggaagga
     1741 aatatataag acagccttgg atatcatcgt tgattgggcg gttgaaattc cggcttacca
     1801 gcgtcaaaat tctacaattt tcagtaccga acgggttaag attgacacgg taacaccgga
     1861 cattaccact ttctatgatt ggaatgccga aattcaa
//
LOCUS       DAOMIV010000442         1892 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_70463_length_1892_cov_3.059336, whole genome shotgun sequence.
ACCESSION   DAOMIV010000442 DAOMIV010000000
VERSION     DAOMIV010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70463_length_1892_cov_3.059336"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PKV59_08890"
     CDS             <1..230
                     /locus_tag="PKV59_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46904.1"
                     /translation="YEIIEAGRPFGMATFDDYIVNLYQKGLITQETAMAYASRKGITG
                     RGIDTVKSARGEATTDIDDLEIDRNYKNRIR"
     gene            283..930
                     /locus_tag="PKV59_08895"
     CDS             283..930
                     /locus_tag="PKV59_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HQP46905.1"
                     /translation="MDITCDKCQSKFKIADEKIPKGKTVTLRCPKCETRISVSSPPPD
                     AAAQTDIIGNVDNSTDESMESAFDFTEEEGKTALVCENNPQIREKIVNILKLMEYHVL
                     EAINARDALTKMRFHIFNLVAINETFDMSNIDNNSILIYLARLNMVIRRNIFVVLLSN
                     QYRTMDKMMAFNKSVNLIINLENIDDADRTLSRSIAENEIFYRVFKEALKKSGRV"
     gene            complement(939..1703)
                     /locus_tag="PKV59_08900"
     CDS             complement(939..1703)
                     /locus_tag="PKV59_08900"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQP46906.1"
                     /translation="MILVVDVGNSNTVFGVYIKDVLAETWRIQTGSHQTSDELGLTVC
                     GLMAADCIDYRDIQKTVIASVVPSLKKTWEGFCRKYLHDAPRWAEASNLSLMPVRCAN
                     PAEVGADRLVNAIAAYHRYQTGLIVIDFGTATTFDVVSEAGEFLGGAISPGIGISADA
                     LHQRTAKLPRLEVFSSPPSAIGRDTMESIRSGVVFGYAGLVDGMVRRIRAELASELKV
                     IATGGLAPAMQGVSETIAAVEPDLTLKGLKIIADRL"
     gene            1702..>1892
                     /locus_tag="PKV59_08905"
     CDS             1702..>1892
                     /locus_tag="PKV59_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46907.1"
                     /translation="MANFPEVFIRDPKRQNPGKKAENKFKSSAPDGLAGHFIHGDPNL
                     SVQKGRLRNHASARRSFSP"
BASE COUNT          526 a          499 c          448 g          419 t
ORIGIN      
        1 tttatgagat tattgaggcc ggaaggcctt ttggaatggc cactttcgac gattatatcg
       61 tgaaccttta ccagaaggga ctcattaccc aggaaaccgc tatggcctat gcgtcccgaa
      121 aaggcatcac ggggcgcggc atcgacaccg tcaagagtgc gcgcggcgaa gccaccacgg
      181 atatcgatga cttggaaatc gaccgaaact acaaaaatag aattcgttaa gttgacgccg
      241 gaaccgccat cgattcaacc atgaaaaact ggagccatca ttatggatat tacctgcgat
      301 aaatgccaga gcaaattcaa gatcgctgat gaaaaaattc caaaagggaa gactgtaacc
      361 ctccggtgtc cgaaatgcga aacccgcata tcggtcagct cacctccgcc ggacgctgcc
      421 gcacagaccg acataatcgg aaatgtcgac aattcaaccg acgagtcgat ggaaagcgca
      481 ttcgatttta cagaagaaga aggaaagacg gccctggttt gtgaaaacaa tccgcagatt
      541 cgtgaaaaaa tcgtcaacat cctgaagttg atggaatatc atgttctcga ggcgatcaac
      601 gcacgtgatg cgctcacaaa aatgcgtttt cacatcttca atcttgtagc gatcaacgaa
      661 acctttgaca tgtcgaatat cgacaacaac agcattctca tctatctggc cagactgaac
      721 atggttattc gccggaatat tttcgtggtt ctgctcagca atcaataccg aaccatggac
      781 aagatgatgg cctttaataa aagcgtaaac ctgatcatca accttgaaaa cattgatgac
      841 gcagatcgca ctctgagccg gagcattgcg gaaaatgaaa tattttaccg ggtctttaaa
      901 gaagccctta aaaaatccgg tcgcgtttga aaggcgcatt ataagcgatc ggcgataatc
      961 ttcaatccct tcagcgtcaa gtccggttcc acagcggcga tggtttccga tacgccctgc
     1021 attgccggcg caagaccgcc ggtggcaatc accttgagtt cggatgccaa ctctgcgcgg
     1081 atacgccgaa ccattccatc caccagaccc gcatatccaa aaacaacgcc ggaacgaatg
     1141 ctttccatcg tatcgcggcc gatagcgctt ggcggagaag aaaaaacttc gagccgggga
     1201 agtttggctg tacgctgatg cagcgcatct gccgagatcc cgatacccgg actgatggcg
     1261 ccgcccagaa attcaccggc ttcggatacg acgtcaaaag tcgtggcggt tccgaaatca
     1321 ataacgatca accccgtttg ataccgatga taggccgcta tggcattgac cagacggtcg
     1381 gcgcccactt cggcgggatt ggcgcaccgc accggcatca ggctcaggtt agaggcctcc
     1441 gcccagcggg gtgcatcatg caggtatttc cggcaaaatc cttcccaggt ttttttcagg
     1501 gacgggacca ccgaggcgat cactgttttt tggatatctc tataatcaat gcagtctgcg
     1561 gccatcaatc cgcataccgt caatccgagc tcatcagagg tttgatgact cccggtttga
     1621 atccgccagg tttccgcaag cacgtctttt atgtaaaccc caaagacggt gttggaattt
     1681 cccacatcaa cgaccagtat catggctaac tttcctgaag ttttcattcg tgacccgaaa
     1741 agacaaaatc ccggcaagaa agccgaaaat aaatttaaat cgagcgcgcc tgatgggctt
     1801 gccggacatt ttattcatgg cgatccgaac ctttccgtgc aaaagggtag actgcgcaac
     1861 catgcttcag cgcgtcgatc attctcaccg tt
//
LOCUS       DAOMIV010000443         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_70728_length_1887_cov_4.360808, whole genome shotgun sequence.
ACCESSION   DAOMIV010000443 DAOMIV010000000
VERSION     DAOMIV010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70728_length_1887_cov_4.360808"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /gene="araD"
                     /locus_tag="PKV59_08910"
     CDS             <1..577
                     /gene="araD"
                     /locus_tag="PKV59_08910"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HQP46908.1"
                     /translation="VTYDAMSVDDMVVIELKTGKIAEGRLNPSSDTPTHLILYQAFPE
                     IGGIVHTHSRWATIFAQMGQGIPPFGTTHADYFYGEIPCTRKMKNEEIQGAPGYYERE
                     TGNVIVERFKDIDPMMIPGVVVHSHGPFAWGENPQEAVHNAVVMEEVAFMAWHMMAVS
                     AKPIPIMQQELLDKHFLRKHGKDAYYGQKSG"
     gene            complement(579..1433)
                     /locus_tag="PKV59_08915"
     CDS             complement(579..1433)
                     /locus_tag="PKV59_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQP46909.1"
                     /translation="MEKRTWKTNLFIVAMLAPSLLISLGVLVYPLLNTVVQSFKSPDT
                     GKYTLENYVYLFTDEISTASIVYTFWITVVTVIVTIAISYLLALYLRFTDNKVSKFIG
                     TIYLLPRFIPSLVAVYAMTTIIRDSGLLNRISLLFGNNFKPGLLYNAKGIILMNIWFN
                     IPFATMIIVSALSGIQDSVIESARDVGANQIRVFFSMILPLSIKDVMIAMTFVFMGNI
                     SSFTTPYLIGPNHPLMLGVYLRRLFSIYHEYELAAATSVVIFLFSSVSAIIYLYTNLK
                     EREWEARN"
     gene            complement(1540..>1887)
                     /locus_tag="PKV59_08920"
     CDS             complement(1540..>1887)
                     /locus_tag="PKV59_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46910.1"
                     /translation="QTLSNLANGTLPDTVKIYQLEDYPPGGTDVVTAVTSIGADPEAC
                     YDFINYVISPEGQKECLELMYAVPVIDISTIQSDAAKMIEGLDVSSFKVFSIGSLGDV
                     INQRWDREIATLP"
BASE COUNT          564 a          405 c          427 g          491 t
ORIGIN      
        1 tgtgacttat gacgccatga gcgttgacga tatggttgta atcgaactca aaaccggaaa
       61 aatagcggaa ggaaggttga acccttcatc cgatacaccg actcatttaa ttctttatca
      121 agcatttccg gaaatcggcg gaatcgtcca tacgcacagt cgttgggcta cgatattcgc
      181 tcaaatgggg cagggaattc cgccgttcgg aacgactcac gccgattatt tttatggcga
      241 aattccctgt actcgaaaaa tgaagaatga agaaattcaa ggtgcgccgg gctattacga
      301 acgcgaaacc ggcaacgtaa ttgtcgaacg ttttaaagat atcgatccga tgatgattcc
      361 gggagtggtc gtacatagcc acggaccttt tgcatggggc gaaaacccgc aagaggctgt
      421 ccataacgct gttgtaatgg aagaagtggc ttttatggca tggcatatga tggcggtcag
      481 cgctaagcct atcccgatca tgcaacagga actgttggat aaacactttt tgcgtaagca
      541 cggtaaagat gcttactacg gtcaaaaatc aggttgagct aatttctcgc ttcccactcg
      601 cgttctttga gattggtgta gagataaatt attgccgaaa cggatgagaa caggaaaatg
      661 acgaccgacg tggcagcggc taattcatat tcgtgataga tggagaataa tcttcgtaaa
      721 taaacgccga gcatcaacgg gtgattcgga ccgattaagt agggcgttgt gaacgaactg
      781 atattgccca taaaaacgaa agtcatcgca atcatgacat ccttaatgga cagcggaaga
      841 atcattgaaa agaaaacccg aatttgattc gctcccacat ctctcgcact ctcgatgacc
      901 gaatcctgaa ttccagaaag cgcggagaca ataatcatgg tagcaaacgg aatattgaac
      961 cagatattca tcaggatgat tccttttgcg ttatagagca atcccggttt aaaattattt
     1021 ccgaataaga gtgatattct atttaacaac cccgaatcgc gaatgatcgt cgtcatggca
     1081 taaacggcga ccaaactggg gatgaatctg ggtaaaagat agattgtacc gataaactta
     1141 ctgactttat tgtctgtaaa gcgaagataa agtgctaaaa gataagaaat cgcaatcgtt
     1201 acgattacgg taactaccgt gatccaaaat gtgtaaacaa tgcttgcggt cgaaatttca
     1261 tccgtaaaga gataaacata attttccagc gtgtattttc cggtatcggg gcttttaaaa
     1321 ctttgaacaa ccgtattcaa taaaggataa accagcactc ccagcgaaat cagcaacgac
     1381 ggtgcgagca tcgcgacaat aaaaaggttg gttttccaag tacgtttttc catgttttat
     1441 tcccctctaa gaaattcttt gggtatggaa tcattctttc ctttgtcggg aatgatgatt
     1501 ccatacccgc ttcaatcgat tacagagtta gcaaatccgt tacggcagcg tcgcgatttc
     1561 acgatcccat ctttggttga taacatcacc cagactgccg atcgagaaga ccttaaagct
     1621 tgagacatcc aaaccctcga tcatttttgc ggcgtcggat tgaatcgtag aaatgtcgat
     1681 aaccggtacg gcgtacatca gttccaagca ttccttttga ccttccggac tgatcacgta
     1741 gttgataaag tcataacatg cttccggatc agcgccgatg gaagtcacag ctgtcactac
     1801 atccgtaccg ccgggaggat aatcttcgag ctgataaatc ttcacggtat cgggcagagt
     1861 tccgtttgcc aagttgctta atgtttg
//
LOCUS       DAOMIV010000444         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_70906_length_1884_cov_3.644068, whole genome shotgun sequence.
ACCESSION   DAOMIV010000444 DAOMIV010000000
VERSION     DAOMIV010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70906_length_1884_cov_3.644068"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..907
                     /locus_tag="PKV59_08925"
     CDS             152..907
                     /locus_tag="PKV59_08925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQP46911.1"
                     /translation="MNRDTIQKLSELNRDFYESFGKAFAATRQRIQPGVRRFLDEDCF
                     DGDWLDLGCGSGALAAAWAESGRTGSYTGVDFSQTLLEEARQLNENISDSRMKLSYFQ
                     ADLSDSSWVRLFENRKFDGICAFAVLHHMPDASARSRIFRQVYSLLKPGGCFCYSVWQ
                     FQNSEKMMRRILPWESIGIQSADVDPGDTLLDWRFSLPDADKKIGLRYVHLFTEGELQ
                     SLAEEAGFLLRKEYFSDGASQNMALYRVEQRKE"
     gene            complement(993..>1884)
                     /locus_tag="PKV59_08930"
     CDS             complement(993..>1884)
                     /locus_tag="PKV59_08930"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQP46912.1"
                     /translation="AQMVPESHLTQINAVDYILRGITGIAGPFLGAVLLRTTTINSIL
                     NVDLIAAAGGSIPLLLISFPKVNSIATHFSVNLVKQIGRSLSDLKSGFYYVRRTPGLF
                     MFLMYVSITNLMLMPAESLLPLVVSTKFHGTEISMSTIGIAFGIGSVLGGIILSIRHG
                     FRSRIKASLTGDLLYGFGIFLIGVASEKQFSLAVFGWALAGIGESMSLANVNALIQAH
                     TRPEMQGRVFSISTSLINISIPVAMVISGPIAEIFEIQFWYIFSGVGILLLAGGMMFV
                     PAMFRLEKPSPAKILDPSYE"
BASE COUNT          535 a          412 c          439 g          498 t
ORIGIN      
        1 tacagacggt tcgcatttag tgagttttga tcaggtagtg gatgtcatga tgcagaccgg
       61 cgtagatatg ccatctttat atcgtgaaac cagtgaaggc ggactggcga agttttttaa
      121 aacacccaaa aagaattcgt cggaataaag cgtgaaccga gataccatcc aaaaactatc
      181 ggagcttaat cgggattttt atgaatcgtt cgggaaagct tttgcggcta cccggcagcg
      241 aatacaaccg ggagttcggc gttttcttga tgaggattgt tttgacggtg attggcttga
      301 tttggggtgc ggatcgggcg ctttagccgc cgcatgggct gaatccggca gaacggggtc
      361 ttataccgga gtcgatttca gtcagacact actggaagaa gcccggcaat taaatgaaaa
      421 tatttccgat agccgcatga aattgtccta ttttcaggca gatttatcgg atagttcttg
      481 ggttcgatta tttgaaaaca gaaaatttga cggaatttgt gcctttgccg ttcttcatca
      541 tatgcccgat gcaagtgccc gaagccggat ttttcggcaa gtttattctt tattgaagcc
      601 cggcggttgc ttctgctatt cggtttggca atttcaaaac tccgaaaaaa tgatgagacg
      661 cattctcccc tgggaatcaa tcggaattca atccgccgat gtcgatcctg gcgatacgct
      721 gttggattgg cgtttttcac ttccggatgc ggataaaaaa atcggactgc ggtatgtcca
      781 cctttttacc gaaggtgaat tgcaatcgtt ggcggaggaa gccggatttc tgcttcggaa
      841 agaatatttc tcagacgggg caagccaaaa tatggctttg taccgagttg aacaacggaa
      901 agaataaatg gatccttttt gtttcggttg tggataaccc gatcgacttc cttcccaaat
      961 caaatcatca tattgccaag aattcaaata aatcattcat acgaaggatc aagaattttg
     1021 gcgggagacg gtttctccaa acgaaacatg gcaggcacga acatcattcc tccggctaat
     1081 aacagaattc ctactccgct gaaaatatac caaaattgaa tttcgaagat ttcggcgatc
     1141 ggtcccgaaa taaccattgc cacgggaata gaaatattga tcaagcttgt cgagatcgaa
     1201 aatactctcc cctgcatctc cgggcgggta tgagcctgta ttaaagcgtt cacattcgcc
     1261 agactcatcg attcaccgat tccggccaat gcccatccga agactgccaa cgaaaattgt
     1321 ttttcggaag ccaccccgat taaaaagatc ccgaaaccat acagcagatc tccggtaagg
     1381 ctcgctttga ttcggctgcg aaatccatgg cgtatgctca aaatgatgcc ccccaatacg
     1441 gaaccgatgc caaatgcaat accgatggta ctcatggata tttcggtacc gtgaaatttt
     1501 gtcgatacga ccaaaggcaa gagactctcg gcgggcatga gcatcaaatt ggtgatagaa
     1561 acatacatca aaaacataaa taagcccggt gttcttcgga catagtaaaa accgcttttc
     1621 agatccgata atgatctgcc gatttgtttt accaaattta ccgaaaaatg ggttgcgatt
     1681 gaatttactt tcgggaacga aatcaacaat aacggaatcg agcctcctgc cgccgcaatt
     1741 aagtcaacgt tcaggataga atttatcgtc gttgttcgta ataaaacagc gccgaggaac
     1801 gggccggcga ttccggttat tccgcgcaaa atataatcaa ccgcattgat ttgggttaaa
     1861 tgactttcgg ggaccatttg tgca
//
LOCUS       DAOMIV010000445         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_70918_length_1884_cov_3.045380, whole genome shotgun sequence.
ACCESSION   DAOMIV010000445 DAOMIV010000000
VERSION     DAOMIV010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70918_length_1884_cov_3.045380"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..1102)
                     /locus_tag="PKV59_08935"
     CDS             complement(23..1102)
                     /locus_tag="PKV59_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQP46913.1"
                     /translation="MKNLMNEHRFDAVALIPGSDFRWLTGTEKHLMERPTMILVTLDK
                     DPAMVAAGFEMNSFDRIGFNFRAFPFSDNPSEWGNAFRQAGEYLDLAGKTIAVDPLHF
                     RFFETKLLENAVTDCRIVSGESLFKKLRLQKTEEEISFMKKAAIIAETALEKTLKIVR
                     PGISEREIASELNAQLMRAGSDSTLPFPPIVAGGPNAADPHAAVSDRILESGDFLLFD
                     WGARYQGYCSDITRTFAVGDISEVQHEVYNTVLAANRAAKRAARPGIPCGAIDRAARD
                     EIEKAGYGSFFTHRLGHGLGMEAHEDPYIYAENSLPLSEGMVFTDEPGIYLPGKFGVR
                     IEDDLVITNEGCEQITSFPRELRIL"
     gene            complement(1205..>1884)
                     /locus_tag="PKV59_08940"
     CDS             complement(1205..>1884)
                     /locus_tag="PKV59_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46914.1"
                     /translation="AEEDYELFVPLHLIYHPDEDHIVISSDLLNQDTAKKADMKKIED
                     RIVREIYLRKDYSQILALNKPMMNSCLQVIDGRNPIYTPAQWSRISEIGSYSQIDKIL
                     TDADPHVPSSEFFGKEPDKNWCYFYEKMSLAQQSEDWEKVAEIAGEALSAGAHGEDPL
                     EWLPLVQAYAYLGRYSDAKPYGEILKTDEYLKFETCRYFRIAAGILNDPSPAKKALPW
                     LENEFCE"
BASE COUNT          480 a          473 c          406 g          525 t
ORIGIN      
        1 tcttcatatt gataagggtt gttcataata ttcttaactc ccgcggaaaa gatgtgattt
       61 gttcgcatcc ttcgtttgtt atgactaaat catcttcgat tctgacacca aacttgccgg
      121 gaagatagat acccggctca tcggtaaaaa ccattccttc gcttaacggt aaactgtttt
      181 cggcgtagat atacggatcc tcatgagctt ccattcccaa gccatgtccc agccggtgcg
      241 taaagaaaga cccatacccc gctttttcta tttcatcacg ggcagcccga tcaatcgctc
      301 cgcagggtat accgggacga gcggctcttt ttgcggcacg gttggcagcc aagacagtat
      361 tgtaaacctc gtgttgaact tcgctgatat cgccgacggc aaaggttctg gtaatgtccg
      421 aacaatagcc ttgataacgg gcaccccaat caaataagag aaaatcaccg ctctccaaaa
      481 tccgatcact gaccgctgcg tgcggatcgg cagcattagg tccgccggct acgatgggag
      541 gaaacgggag tgtcgaatca cttcccgctc gcatcaactg cgcattcaac tccgatgcga
      601 tttcgcgttc gctgatgccc ggtcgaacga ttttcaatgt cttctcaaga gctgtttcgg
      661 cgataatcgc cgcttttttc atgaaagata tttcttcttc cgtcttttgt aaccgtaatt
      721 ttttaaagag agattctccg gaaacaattc gacaatccgt aacggcattt tccaaaagct
      781 tggtttcaaa gaagcgaaaa tgaagcgggt cgacggcaat tgtttttccg gctagatcca
      841 ggtattcgcc cgcctgtcga aatgcgtttc cccactcgct cggattgtcc gaaaaaggaa
      901 atgctctgaa attaaatccg atccggtcaa aagaattcat ttcgaatccg gcggcaacca
      961 tagcgggatc cttatcaagc gtcaccaata tcatggtagg tcgctccatc agatgtttct
     1021 ccgttccggt caaccagcgg aaatcggatc cgggaatcag agcgacggca tcgaaccgat
     1081 gctcgttcat aagatttttc aatttttcta aattcgtttt tgtcaaggtt ttttccttta
     1141 aataatattg atcaaaaata actattgaaa gatgcttact tttcagccgg acaacgacac
     1201 ccttctattc acagaactca ttttcgagcc atggcagggc ttttttcgcc ggagacggat
     1261 cattcaagat accggcggcg attcgaaaat aacggcaggt ttcgaatttc agatactcat
     1321 ctgttttcag aatctcccca tagggtttgg catccgaata tctgccgagg taagcatacg
     1381 cctgtaccaa gggcagccat tccaacggat cttccccgtg agctccggct gacagcgctt
     1441 cgccggcgat ctccgcaact ttttcccaat cctccgattg ctgtgcgaga gacatctttt
     1501 catagaaata gcaccaattt ttatcgggtt ctttgccgaa gaattccgaa gacggaacat
     1561 gcggatccgc atcggtcaaa atcttatcta tctgagaata acttccgatt tccgaaatcc
     1621 gtgaccattg tgccggcgta tagatcgggt tccgtccgtc gataacctga agacaagagt
     1681 tcatcatcgg tttgttcagt gccagaattt gcgagtaatc cttgcgcaaa tagatttccc
     1741 tcacaattcg gtcttctatc tttttcatgt cggctttttt ggctgtatcc tgatttagta
     1801 aatccgaact gatcacgata tggtcctcgt ccggatgata aatcaaatga agcggaacaa
     1861 aaagttcata atcctcttcg gcgg
//
LOCUS       DAOMIV010000446         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_70949_length_1884_cov_1.835976, whole genome shotgun sequence.
ACCESSION   DAOMIV010000446 DAOMIV010000000
VERSION     DAOMIV010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70949_length_1884_cov_1.835976"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PKV59_08945"
     CDS             <1..1030
                     /locus_tag="PKV59_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HQP46915.1"
                     /translation="EEKGLVIGLLPTWGDKWNKKWGVGPEIFTPENAAVYGEILGKRY
                     KNFLNIIWILGGDRPIEKDEHREIVRAMASGLRKGDGGAHLITFHPMGGHGSAEYFHE
                     EEWLDFNMRQNGHTPNFTGSYDNTLKDYKRMPAKPVMDGEPLYEDHPIEFNAEKNGHS
                     VSADVRRPLYWDLFGGAFGHTYGHHSVWQMWHPDRDPINFPLMPWYEAVDQPGAWQMQ
                     FGRRLMESRPFLTRIPDDSVIVPDEVKTSVPGAGRYRFVATRDENGRYAMVYVPVGRP
                     FTVRMDVVSGKKVTAWWYNPRNGEAQKIGNFFNEGTRTFVPPQPGENLDWVLVLDDAA
                     AGFKAPGE"
     gene            complement(1210..>1884)
                     /locus_tag="PKV59_08950"
     CDS             complement(1210..>1884)
                     /locus_tag="PKV59_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46916.1"
                     /translation="YSRIIRGFEELGMVVQKIDKLSLRQDLDELLEVYSDRPFKTISM
                     MRLNQQILEVMNRHRMSVPSNMVMMLRTMSIAEAIGKQLEPDFDLFTLVQPFVRKVYL
                     NINSPQNWKNRSTIMIDESAQLIEHLPHNLSELLRKLRDGQFEVKLEHHNLENLTREM
                     KGSSNRISASLIIAALIVGSSLILQQGIGPLVSNISILGIIGYILAGILGLGLLVAIL
                     RSSRKK"
BASE COUNT          519 a          374 c          470 g          521 t
ORIGIN      
        1 ggaagaaaaa ggcctggtta tcggcctgtt gcccacttgg ggagataaat ggaacaagaa
       61 atggggcgtc ggccccgaga ttttcacccc ggaaaatgcg gcagtttatg gggaaattct
      121 cggcaaacgg tataagaatt ttctcaatat catttggatt ctcggcggag accgacccat
      181 tgaaaaggat gagcacagag aaatcgtcag agctatggct tcgggtttac gcaaaggcga
      241 cggtggtgct catttgatta cttttcatcc gatgggagga catggttcgg ccgagtattt
      301 tcacgaggaa gagtggctcg atttcaacat gcgtcagaac gggcatacgc cgaatttcac
      361 gggaagttat gacaatacgc tgaaagatta taagcgtatg cccgcaaaac cggtaatgga
      421 tggtgaaccg ctttatgaag atcatcccat tgaattcaat gcggaaaaaa acgggcattc
      481 tgtttctgcc gatgttcgac gtccgcttta ctgggacttg ttcggcggtg cattcggaca
      541 cacctatgga catcattccg tttggcagat gtggcatccg gaccgtgatc cgatcaattt
      601 tccgttaatg ccgtggtacg aagccgttga tcagccgggt gcatggcaga tgcagttcgg
      661 caggcggttg atggagtcga ggcctttcct cacacgtatt cccgatgatt cggtaattgt
      721 acctgatgag gtaaaaacct cggtgcccgg tgccggacgt tatcgttttg tagctacccg
      781 agatgaaaac ggccgttatg ccatggttta tgtgccggtg ggcaggccgt ttacggtaag
      841 gatggatgtt gtttcgggga aaaaagtaac agcatggtgg tataatccac gtaatggaga
      901 ggctcagaaa atcggaaatt ttttcaatga aggaactcgt acatttgtac cgccgcaacc
      961 gggtgaaaat ttagactggg tgttggttct cgacgatgca gcggccggtt ttaaagctcc
     1021 cggagaataa tttgacagag ttagctattt ttgaataaac ttcgacgctg tcgttatcga
     1081 tatgtgcgga ttgccgccat acttgttgaa atgactttga caaaaattaa gaatgaaaat
     1141 atctttgtga atatcagata attatatcga ttgtaatttt tatcatcggg caaaaaagga
     1201 gaaaagaatt tatttttttc gactcgatcg taaaatagca acgagaagac ccaatcccaa
     1261 gattcctgca agaatataac cgataatccc taaaatggaa atattcgaga ccaaaggacc
     1321 aattccttgt tgcaaaatca gggaagatcc tacgataagt gcagcaataa tcaggcttgc
     1381 cgaaatgcgg ttgcttgaac ctttcatctc acgggtaaga ttttccaggt tgtgatgctc
     1441 gagttttact tcaaattgtc catccctcag ttttctgagc aattcagata aattgtgtgg
     1501 cagatgttca ataagttgtg cactttcatc gatcatgatt gtgcttcgat ttttccagtt
     1561 ttgaggggag ttaatgttga gatatacttt tctaacgaaa ggttgtacga gggtaaacaa
     1621 atcgaaatcg ggctccaatt gtttaccgat tgcttcggca atcgacatgg tgcggagcat
     1681 cattaccata ttggaaggaa ctgacatccg gtgtcgattc ataacctcta aaatctgttg
     1741 attgagccgc atcatgctga tcgttttgaa aggacgatcg gaatatactt cgagtagttc
     1801 gtccaaatcc tgacggagcg atagcttatc gattttctgt acaaccattc ccaattcctc
     1861 aaatcccctg atgatgcggg aata
//
LOCUS       DAOMIV010000447         1877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_71283_length_1877_cov_4.546103, whole genome shotgun sequence.
ACCESSION   DAOMIV010000447 DAOMIV010000000
VERSION     DAOMIV010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71283_length_1877_cov_4.546103"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PKV59_08955"
     CDS             <1..974
                     /locus_tag="PKV59_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46917.1"
                     /translation="PWLAAVQPTLLPSNTASPSPILLLNNQTNSAPTETVFIPTDEPT
                     AIPVPPTEVLSAPIDTSNSPTETPLPFYRPVIVDPNPSGADWKSLPIIPESITETAKD
                     IYRYGVSKMGRRPNYFSKIGDCQSYPNVFMGEYDYVSGSYELSDDDLYLEDAINYFQE
                     SFGALSFAVANGLSAASLLTTIWSDPTYCQNGESALLCELRINKPSIVFVNLGTNWVS
                     TADVSLYEDYLDEIVYTIIQYGTLPILSSKADNVEGNNQINEITAAIAKKYGVPFFNF
                     WAAAQSLENHGLDPDRSNIYLSVDAWKVRSYWGLKMLYTVGHSLELF"
     gene            1021..1413
                     /locus_tag="PKV59_08960"
     CDS             1021..1413
                     /locus_tag="PKV59_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46918.1"
                     /translation="MKKIIYSLAMIILCVQLSGCGGNKKVSEESKVVESFYNAIVNQE
                     RDKIGSIVCSDWEKDGLREVDAFMGVKSDLKDFSCSVQKEEADNAEVTCTGSIAASYG
                     NEITEFPLKDRIHKVVKENGEWRLCGFE"
     gene            1537..>1877
                     /locus_tag="PKV59_08965"
     CDS             1537..>1877
                     /locus_tag="PKV59_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46919.1"
                     /translation="MLLSNILLLAGLFLLIRGIPQQRIRKYFLLALSIIFVFCLQPTL
                     SIRNFDFWLPLTTTGLTLVCWAFCCRSDTSRLSDNRSELLIIFGTVLFIALSRLFSFE
                     GIFTASRPPILS"
BASE COUNT          481 a          402 c          429 g          565 t
ORIGIN      
        1 atccttggct tgcagccgtt cagccgactc ttttaccgtc caatacagct tctccctcac
       61 ccatcctgtt gttaaataat caaacgaact cggctccgac cgaaacggtc tttattccaa
      121 ccgatgaacc caccgctatc ccggtaccgc cgacggaagt gctgtctgcc ccgatcgata
      181 catccaattc cccgactgaa actccgcttc ctttttatcg gccggtgatc gtcgatccga
      241 acccatcggg agcggattgg aagagcctgc ctatcattcc ggaatcgata acggagaccg
      301 cgaaagatat ttatcggtac ggagtgtcga agatgggaag gcgcccgaat tatttttcaa
      361 aaatcgggga ttgccagtca taccccaatg tattcatggg cgagtacgat tatgtttccg
      421 gctcttatga attaagtgat gatgaccttt atttggaaga tgccatcaat tattttcaag
      481 aatcgtttgg cgctttgagt ttcgcggtcg cgaacggtct gagcgccgct tctttgctga
      541 caaccatctg gtccgaccca acttactgtc agaacggtga atccgcattg ctttgcgagc
      601 tgcgaatcaa taaaccttcg attgtttttg ttaatttagg cacgaactgg gtatcgaccg
      661 cagatgtatc gttgtatgaa gattatttgg atgaaattgt ttatacgatt attcaatatg
      721 gaacgttgcc gattctttct tccaaagcgg ataatgtgga agggaataat cagatcaatg
      781 agatcacagc cgctatcgcc aaaaaatacg gtgtaccatt tttcaatttt tgggcggcag
      841 cccaaagttt ggaaaatcac ggcttggatc cggatcgttc caatatttat ctttcagtcg
      901 atgcatggaa agtccgcagc tattggggat tgaaaatgtt atatacggtc gggcattctt
      961 tggaactttt ttgatctggg gaagcgatcg aacgatttat aatagcaggg ggagaaagtt
     1021 atgaaaaaaa taatttattc gctcgcaatg attattctgt gtgttcagct ttccggatgt
     1081 ggcggaaata aaaaagtttc cgaagaatcc aaggtagtgg aatcttttta taatgcgatc
     1141 gtgaatcaag aacgggacaa gatcggatcg atcgtttgtt ccgactggga aaaagacggt
     1201 ttacgggaag tcgatgcttt catgggagtg aaatcggatt tgaaagattt cagctgctcc
     1261 gttcaaaagg aagaggctga caatgccgaa gtgacatgca cgggatcaat cgccgcttct
     1321 tatgggaatg aaataacgga atttccgctg aaagacagaa tccataaagt ggttaaagaa
     1381 aacggagaat ggcggctttg cgggttcgaa taggattttt cagtgagact gttcaatcgt
     1441 gaaacattaa tcgctttcct ttttgctctt ctcattatcg gcttaatcat ctttacagcg
     1501 gatgaagccc cacaatgggt ttatcaggga ttttaaatgc ttttgtcaaa tattctgtta
     1561 ttggcggggt tatttttatt aatccgcggc atccctcagc aaagaattcg aaagtatttt
     1621 ctgctggcgt tatcgatcat ttttgtattc tgccttcaac ctaccctttc catccgtaat
     1681 tttgatttct ggttacctct cacgaccacc ggcctaaccc ttgtatgttg ggcattttgt
     1741 tgcagaagtg ataccagccg actttccgat aaccgatcgg agcttttgat tattttcgga
     1801 acggtattgt ttatcgcttt atcgcgtcta ttcagttttg agggaatttt taccgcatcc
     1861 cgtcccccga tcctttc
//
LOCUS       DAOMIV010000448         1871 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_71644_length_1871_cov_5.157489, whole genome shotgun sequence.
ACCESSION   DAOMIV010000448 DAOMIV010000000
VERSION     DAOMIV010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71644_length_1871_cov_5.157489"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="PKV59_08970"
     CDS             <1..1231
                     /locus_tag="PKV59_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46920.1"
                     /translation="LILIFHRKDTGKRRFRNFVVFTSSILIVLAPALIRSVSLTGSVA
                     LEDMGIHNYELARRLSCDPNWQPTSISGESEAAFAERMKQEMIGFLIDHTGKIVQYTL
                     NHFVKDLVDSWLVLPVGIPPGFTVLNITDSAYQDVPGRLAGGSLVLFAVCAFLIASGI
                     ASLWKRHGIAGLLPLILCPLYMLTAAVGRYSGWRFILPADWMIYFYFCCGVFEFVFWF
                     RSRFIEIAVLESTEARNVTGILHRKKLLWNVLLILFILLGWAPPFSGIIPDQLKLNST
                     AENKALIQQIGEHYGGVYADLSTEVNQQDWDVINGRTIYPRWFTAGDGLTSANPWVVN
                     EIRDFNRLSFVLLNEKNQDVILPLRQPPEAVPHRADCFVVGSFTEEGWFEGKAVVFPD
                     TLDSSGRPIIILSNKRD"
     gene            complement(1375..>1871)
                     /locus_tag="PKV59_08975"
     CDS             complement(1375..>1871)
                     /locus_tag="PKV59_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQP46921.1"
                     /translation="FEVKPGLKIAILNTLGDTELVEACATEIARRLENVDYDVLVTAE
                     SKSIPFAHALSVRTKKPYVVCRKNYKSYMGRALESTSNSITTQLEQHLILDEKDIPLV
                     KGKKIVIVDDVISTGSTMHAIQTLMEMAGSDPNYTYAAICTEGNEHDWENIISLGHLP
                     LFEG"
BASE COUNT          458 a          404 c          450 g          559 t
ORIGIN      
        1 gttaatatta atttttcatc gaaaggacac agggaagcgg cggtttcgga atttcgtggt
       61 gtttacatct tcgattctga tcgttttggc tcctgccttg attcgcagcg tctcattaac
      121 cggaagtgta gctttggaag atatgggaat ccataattat gagctggcaa gacgattatc
      181 ctgtgatccg aattggcaac cgacctcaat atccggcgaa agtgaagcgg catttgccga
      241 acgtatgaag caggaaatga tcggctttct aattgatcat acggggaaaa ttgttcaata
      301 tacactgaat cattttgtga aagatttggt cgacagttgg ctggtgctgc cggtaggaat
      361 tccgccgggg ttcaccgttt tgaatatcac cgattcggca tatcaggatg ttccggggag
      421 acttgcagga ggatcgctcg ttctatttgc cgtctgtgca tttttgattg cgagtggaat
      481 tgcatctctt tggaaacgtc acggcatcgc cggtctgctc cctttgattc tttgtcctct
      541 atacatgctg acggctgcag tggggcgtta ttccggctgg cggtttatat taccggctga
      601 ttggatgatt tatttttatt tctgttgcgg agtattcgaa tttgtctttt ggttccgatc
      661 gagattcata gagattgccg ttctcgaatc gaccgaagcg agaaacgtaa cgggaatctt
      721 gcatcggaaa aagcttttat ggaacgtctt attgatcctg ttcattctcc tcggatgggc
      781 gccgccgttt agcggaatca ttccggatca gcttaaactc aattccaccg cggagaataa
      841 ggcgctcatt caacaaatcg gtgaacatta cggaggcgtt tacgcagatc tttctaccga
      901 ggtgaatcaa caagattggg atgtgattaa cggtcggacg atttatcctc gttggtttac
      961 agccggggac ggattaacca gtgccaaccc gtgggtggtt aatgaaattc gtgattttaa
     1021 tcggctgagc tttgtccttc tgaatgaaaa aaatcaggat gtgattttac ctctgcggca
     1081 gcctcccgaa gcggttcctc atcgggcgga ttgtttcgtc gtcggcagtt tcacggaaga
     1141 aggttggttc gagggaaaag cggtcgtttt ccccgatact ttggattcaa gcggcagacc
     1201 gataatcatt ctgagcaata aaagagatta aacaaatttg ctgtgttgaa agaagccggg
     1261 acgtcgattg gcgccccgct atgaataact cacttctata ttaattccct tcttttcgtt
     1321 cggaaatcac cttcgaaaac agatcgaacc aatcaggaaa tatttccgtt acgattagcc
     1381 ttcaaacaga ggaagatgac ccagggagat tatattttcc cagtcgtgct cattcccttc
     1441 ggtgcaaatc gcagcatacg tgtaattcgg atcgcttccg gccatttcca tcagtgtttg
     1501 aatggcatgc atggttgatc cggtgctgat gacatcatcc acaataacaa ttttcttccc
     1561 ctttaccaaa ggaatatctt tttcatccag aatcaaatgt tgttccagtt gggttgtaat
     1621 ggaattcgaa gtggattcaa gcgcccgtcc catataggat ttgtaatttt ttcggcagac
     1681 tacataaggt tttttcgtgc gaaccgataa tgcatgtgcg aatgggatgc tcttcgattc
     1741 cgcggtaaca agcacgtcat aatcaacatt ttccaaccgc cttgcgattt cggtcgcaca
     1801 agcttccacc aattccgtat ccccaagggt attcagaatc gcgattttta agccgggttt
     1861 cacttcaaat a
//
LOCUS       DAOMIV010000449         1842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_73405_length_1842_cov_3.726917, whole genome shotgun sequence.
ACCESSION   DAOMIV010000449 DAOMIV010000000
VERSION     DAOMIV010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73405_length_1842_cov_3.726917"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="PKV59_08980"
     CDS             <1..1632
                     /locus_tag="PKV59_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQP46922.1"
                     /translation="LFESAVGKFSGTEFIQSKQVNPKWFKKKRTGKVIVPDGRFIQEL
                     FVKQVRNSGNSPLASDQMRGTISYREAAIGIYILKKKFMKFKEPHIGLMFPASASFPV
                     IFLSVLFSGKIPVLVNWTTGSRSVVEMMDMLNVRSIITSRVFTEKLSSLGTDLSALND
                     RFIYAEDVASSVKLTTKISSAIKIRLFPGLLHGKSYPDTAAILFTSGSESIPKAVPLT
                     HENILSNIRDAINSFTLYNRDSFIGILPPFHSFGLTVTILLPALCGLKTCYHPNPTEG
                     RIIAKLINAYKTSVMIATPTFLDTIAQNAEPEDLEALRLVVAGAEKCPLRLYDYIENN
                     WPKLRIIEGYGITECSPVVSVNREDNPVRGSIGKLLPSFEYSIINPDTGEQVNEGESG
                     ILLLRGPSVFGGYLNHSGKSPFITFNNLQWYNSGDIVKEEHGSLFFTGRLKRFIKLGG
                     EMISLPLIEDILSDSFRGNEGEVVIAVDSNANDDNPEIILFHSIDLTRTEANSILREK
                     GLSPIYFIRKTIKIDEIPKLGSGKTDYRKLRSLVS"
     gene            complement(1612..>1842)
                     /locus_tag="PKV59_08985"
     CDS             complement(1612..>1842)
                     /locus_tag="PKV59_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46923.1"
                     /translation="RDKLRAAAPFSFVHFFWAKQKKWTNGFKRCGAPFDLSLNLGERM
                     ISKAKPLTTSSVTRQIGFKRCLHLLLSADKRP"
BASE COUNT          557 a          395 c          373 g          517 t
ORIGIN      
        1 ctatttgaat ccgcagtcgg gaaattttca gggacggaat ttattcaatc aaaacaggtt
       61 aatcctaaat ggttcaaaaa aaagcgcacc ggcaaagtaa ttgttccgga cggcagattc
      121 attcaggaac tttttgttaa acaggtacga aattccggaa acagtccctt ggcatcagac
      181 cagatgagag gaactatttc atacagagaa gcggcaatcg gaatttacat tctgaaaaaa
      241 aaattcatga aattcaaaga gccccatatc ggattgatgt ttccggcatc ggcttcattt
      301 ccggttattt tcctatcagt actcttctca ggcaaaatac ccgttcttgt caattggact
      361 acaggctccc gcagtgttgt tgaaatgatg gatatgctca atgtccggtc tataataact
      421 tcgcgtgtat ttactgaaaa attatcatca ttgggaacag atctttcggc acttaacgac
      481 cgtttcattt acgccgaaga tgttgcatcg tcagtaaaac ttacaacaaa aatatcatcc
      541 gcaataaaga taagattatt ccccggattg ctccacggta aaagctatcc cgatacggca
      601 gcaattcttt tcacaagcgg gtcggaatca attccgaagg ctgttccgct tacgcatgaa
      661 aacatacttt cgaatatccg tgacgcaata aactcattta ctctttataa tcgggattcc
      721 tttatcggaa tacttccgcc gtttcattct ttcggtctga cggttacgat tctgcttcct
      781 gcgctgtgcg gattaaaaac atgctatcat ccgaatccaa ctgaaggccg tataatagcc
      841 aaacttataa acgcatacaa gacttctgtc atgatagcaa ctccgacgtt tcttgatact
      901 atcgcacaaa atgcagaacc cgaggatctc gaagctttgc gcctcgttgt agcaggggca
      961 gaaaaatgtc cgttgcgcct gtacgactat atcgaaaaca actggccgaa acttagaatt
     1021 attgaaggat atggcataac agaatgttct ccggttgttt ccgtaaaccg tgaagataat
     1081 ccggtacgcg gaagcatcgg aaaacttttg ccgtcgtttg aatattcaat cattaaccct
     1141 gataccggcg aacaagtaaa cgaaggagaa tcgggaattc tgctcttaag aggtccaagc
     1201 gttttcggag gatatttaaa tcattcaggc aaatcgccgt ttataacttt caacaacctt
     1261 caatggtata attcaggaga tattgtaaaa gaagaacatg gttcgctttt ttttaccggc
     1321 aggctgaagc gcttcatcaa actcggcggc gaaatgattt cacttccgct tatagaagat
     1381 attctgtcgg attctttcag gggcaatgaa ggtgaagttg tcattgcggt tgattcaaat
     1441 gcaaatgatg ataatcctga aataattctt tttcatagta ttgacctgac acgcactgaa
     1501 gcaaattcaa tcctcagaga aaaaggactt tcaccgattt attttatccg aaaaacaatt
     1561 aaaattgatg aaataccgaa actcggcagc ggcaaaaccg attataggaa attacggtcg
     1621 cttgtcagct gataacaaaa ggtgcaagca ccttttaaat ccgatttgtc tggtcaccga
     1681 acttgttgtc aaaggcttcg cctttgatat cattctttcc ccgagattca gggataaatc
     1741 aaaaggtgct ccgcaccttt taaatccgtt tgtccatttc ttttgtttcg cccaaaagaa
     1801 atggacgaaa gaaaagggcg ccgctgcgcg gagcttgtcg cg
//
LOCUS       DAOMIV010000450         1836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_73803_length_1836_cov_4.484559, whole genome shotgun sequence.
ACCESSION   DAOMIV010000450 DAOMIV010000000
VERSION     DAOMIV010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73803_length_1836_cov_4.484559"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PKV59_08990"
     CDS             complement(<1..183)
                     /locus_tag="PKV59_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQP46924.1"
                     /translation="MKIYHSVTELIGHTPLLELSRYETVHNLSATILAKIESFNPTGS
                     VKDRPALAIINRAEADG"
     gene            complement(484..1437)
                     /locus_tag="PKV59_08995"
     CDS             complement(484..1437)
                     /locus_tag="PKV59_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46925.1"
                     /translation="MNVEAIIIDGMAFALPIFIMAIGGIYSEKSGITMLALEGLQGFG
                     AFTGAIAVLILSPVIGENSPKLIYIAIFAAMIGGMIYSMLHAVLSIHFLANQVISGVV
                     VNILATALTTFLTSLLNESLTGEASNKIQLGAAPRFTVPYLSSVPILGAFFKDVYIFV
                     PFIAFFALIAWYVLYKTPYGLRLRACGENPYSVDAAGGNVNGIRFSAVMICGFLSGLA
                     GIFFAYSTSANFSPSIYMGYGYLAIAALIFGNWKILPTLGVCVFFGLAKSGGYQLTLA
                     LGLSSKVSDLFLTIPYILTLLLLIFFSKHNHPPKAVGQPFD"
     gene            complement(1434..>1836)
                     /locus_tag="PKV59_09000"
     CDS             complement(1434..>1836)
                     /locus_tag="PKV59_09000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46926.1"
                     /translation="KVGRGLVRAMAISGMLAGLAGVTMYLGYYNTIVPKELSSMGYDS
                     IAVALLGNLSPSGCIFASFLITIFQKGAVYMSSSTGVAKEIASVITGILLLFSATGMY
                     IKYVAQRTRAKQSVLPTSNGNSQNGNGGNRI"
BASE COUNT          526 a          422 c          425 g          463 t
ORIGIN      
        1 accgtctgct tcagcccggt tgattatcgc cagagcaggg cgatctttga cgcttccggt
       61 gggattgaaa ctttcgattt tcgctaaaat tgtggcggac aagttgtgaa ctgtttcata
      121 acgggacagc tccaataacg gtgtatgacc gatcagttcc gtaacagagt gataaatttt
      181 catgtgtttt tctcctttca aataaaaaaa gcagcaggtt tttttattcc tgccgcttcc
      241 cgaaccccgt gattatttgt gttaatgaat atcaactctt ctttaacaga aaagcagcag
      301 ataagatccg aaacaacaac agatgcaatt gatgacgacc tgatttggaa ctcttcccgc
      361 tgatttcatt ttattttcct tcataattcc gttttgaata tatcatgcct gctatttcgt
      421 gtcaattctc ctctgaaaaa tcaggatatt tttcattcgg tttcgcagtg tttgatgcca
      481 ctcttaatca aacggttggc cgaccgcttt cggaggatgg ttatgttttg agaagaagat
      541 cagcaataac aaggtcagga tataagggat tgtcaagaac agatccgaaa ctttgctgga
      601 gagacccaat gccaaggtca gttggtaacc tccggatttt gccagaccga aaaacacgca
      661 aactcccaag gtcggtaata tcttccaatt tccgaagatc aacgccgcga tggcaaggta
      721 accgtatccc atgtaaatgc tgggcgaaaa atttgcggag gttgaatagg cgaaaaagat
      781 gcccgccaat ccggagagaa aaccgcagat catcaccgcg ctgaaacgga ttccgttgac
      841 gttcccgccg gcggcatcga cactgtatgg attctcaccg caggcacgta accgtaaacc
      901 gtaaggggtt ttatagagaa cataccaggc gataagcgca aagaacgcga tgaagggaac
      961 aaagatataa acatctttga aaaatgcccc caatatcggg acagaagaca ggtaaggaac
     1021 ggtaaaacgg ggtgcggctc ccaattgaat cttattcgaa gcttctccgg tcaatgattc
     1081 attcagcaga ctggttaaaa aggttgtcaa agcggtagcc agaatattga caaccacgcc
     1141 gctgatgact tgatttgcca gaaagtgaat gctcagcacg gcgtgaagca tggaatagat
     1201 catgcctccg atcatcgcgg cgaatatcgc gatataaatt aatttcggcg agttttctcc
     1261 gattaccgga gaaaggatca gcaccgctat tgcgccggta aatgcgccaa atccttgcaa
     1321 tccttcgaga gccaacatgg ttatgccgct tttttcactg taaatgccgc cgatcgccat
     1381 gataaaaatc ggtaatgcga atgccattcc gtcgatgatg atcgcttcaa cgttcatatg
     1441 cgatttcctc cgtttccgtt ttgactgttt ccgtttgagg taggaagaac ggattgtttt
     1501 gcccgggtac gctgcgcgac atatttaata tacattccgg tcgcactgaa aagcaaaaga
     1561 atgccggtaa taacagaggc gatttctttg gcaacgccgg tagatgagct catataaacc
     1621 gcccctttct gaaagatcgt gatcagaaat gaagcaaaga tacaaccgga agggctcagg
     1681 ttgcccaaaa gagcgacggc gatcgaatca tatcccatgg aagaaagctc tttcggaacg
     1741 atggtattgt aataacccag gtacatcgtg actcctgcca aaccggcgag catgccggaa
     1801 atcgccatcg ctcgaaccag tccccgaccg actttg
//
LOCUS       DAOMIV010000451         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_74072_length_1832_cov_3.079347, whole genome shotgun sequence.
ACCESSION   DAOMIV010000451 DAOMIV010000000
VERSION     DAOMIV010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74072_length_1832_cov_3.079347"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PKV59_09005"
     CDS             complement(<1..1345)
                     /locus_tag="PKV59_09005"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HQP46927.1"
                     /translation="MKGSAINQKTIPHRIYLTEDQIPTQWYNIRADMKNKPAPILNPG
                     TFKPLSKEELTPIFAEKLVDQELDNETRYIDIPDGIREIYKIYRPSPLIRAYNLEKAL
                     DTPAKIYYKYEGNNTSGSHKLNSAIAQAYYAKEQGLKGMTTETGAGQWGTALAEACSL
                     FGIPLIVYMVKVSYDQKPFRRTVMQTFGAEVHPSPTTQTQIGKKILAENPETTGSLGC
                     AISEAVETAVTHEGYRYFLGSCLNYVILHQSIIGIESKIAMESIGEYPDIVIGCAGGG
                     SNFGGLISEFVHDKLTGKSNPRLIAIEPASCPSFTRGKFAYDFADTGKITPLCKMYTV
                     GSQYIPPSDHAGGLRFHGMSPILSQLFHDGIIEAQAVTQRKVFEAAVLFAKTETILPA
                     PESAHAILGAINEALKCKESGEEKTILFGLTGTGYFDMTAYEAYLNGSMNDSSISE"
BASE COUNT          464 a          417 c          391 g          560 t
ORIGIN      
        1 cctcgctgat cgatgaatcg ttcatactgc cgttgagata agcttcataa gctgtcatat
       61 cgaaatagcc tgtgccggtc aagccgaaaa gaatcgtctt ttcctccccg gattctttgc
      121 atttcaaggc ttcattgatt gctcccaaaa tggcgtgagc ggattccggt gccggcaaga
      181 ttgtctctgt tttggcaaaa agaaccgcag cttcaaatac ctttctttgg gtgaccgctt
      241 gagcttctat aattccgtca tgaaataact gggaaaggat gggggacatt ccatgaaagc
      301 gtagtccgcc ggcatgatca gaaggcggaa tgtattggct gccgacggta tacattttac
      361 acagcggagt aattttaccg gtatcggcaa aatcatatgc gaattttcct cgggtaaaag
      421 aggggcaaga agccggttcg atcgcaatta accgcggatt tgactttccg gttaatttat
      481 catggacaaa ttccgaaatc aatccgccga agttggaacc tccgccggca catccgatga
      541 caatgtcggg atattcgccg atagattcca tcgcaatttt cgattcaatc ccgatgatcg
      601 attgatgcaa gatcacatag ttcagacacg aaccgagaaa atagcgatat ccttcatgtg
      661 tgacagctgt ttccaccgct tcactgattg cgcaacctaa actaccggtc gtttccggat
      721 tttcagctaa aattttcttt ccgatttgcg tttgagttgt cgggctggga tgaacttcgg
      781 caccaaaggt ttgcataact gtgcggcgaa agggtttttg atcatagctg actttaacca
      841 tatatacgat caaagggatt ccgaataaag agcaagcttc cgccaatgcc gttccccatt
      901 gtccggcacc ggtttctgta gtcatccctt tcaatccctg ttctttcgca taatatgctt
      961 gagcgatagc cgaatttaac ttatgacttc cggatgtatt attcccctca tatttgtaat
     1021 aaattttggc gggagtatca agagcttttt ccaaattgta agcccggatt aacggcgacg
     1081 ggcgatatat tttgtagatt tcccgaattc cgtccggaat atcgatatat cgggtttcgt
     1141 tatctaattc ctgatccacc aatttttcgg caaaaatcgg agtcaactct tctttggaaa
     1201 gcggtttgaa tgttcccggg tttaaaatcg gagcaggttt atttttcatg tctgctcgaa
     1261 tgttatacca ttgcgtcgga atttgatctt cggtaaggta gatacggtga ggaatagttt
     1321 tttgattaat tgctgagcct ttcattttat ttctcctttt gattcttacc ggggaaacaa
     1381 aaagaccttt gtccccggtc tgattaaacc tgggacaaag gtcgtaaaac tcaacctctg
     1441 cggtaccacc catcttgacg tgttctcacg cccgcttatt ctacgcacca tcatgcgtac
     1501 tcccttgata acgggtggag ttcccgtcag gcactacttg caaattgctt tcgtcctgcc
     1561 ctcctgagtc cattcagttc ggctgccggc tgttgctctc tcaccattta tgcaactctc
     1621 tgtatgctgc gttgctgaac ctactattct caatcaacgg ttatttctta ttgattgcag
     1681 gcattttaaa ttccggatga agttatgtca ataccgaatt cggtaaaaat atgacaaatc
     1741 cagaaataac ttataatcca tggtatggaa aatgaagttt atcgtttcga aacaaaatta
     1801 ccggtttctg tctatatttt atccggagaa cc
//
LOCUS       DAOMIV010000452         1825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_74468_length_1825_cov_4.333898, whole genome shotgun sequence.
ACCESSION   DAOMIV010000452 DAOMIV010000000
VERSION     DAOMIV010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74468_length_1825_cov_4.333898"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PKV59_09010"
     CDS             <1..1367
                     /locus_tag="PKV59_09010"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQP46928.1"
                     /translation="REEDIICTSKRHPATMRYRAAIKDNRIAAIQADVVYNCGAYTTL
                     SPVVLQRGLIGATGVYTFDNIRVEGKGVKTNTTPNGAFRGFGGPQTFFAIEMFMNHLA
                     KEVGSDPLPFKRSYLSKQGDTTATNGKYHFYIPMPEMLEKILKASDYERKYESYKRQT
                     GRYRKGIGLSMVYHGCGFTGSGERDMIKGVVKLHKNVNNTVEILTAGTDMGQGLKTTF
                     SKIVAKRLEIPIDQIIIRNPDTDRVPNSGPTAASRSLMVVGRLLDKAALKLKECWVDE
                     EEQEITERYVHPDFVIPWDLNTFQGDAYPDNAWSVNVVEVEVDTLTAVSKVIGAWGVY
                     DVGTPIDMAIIQGQMQGGFLQGIGYGSIELMDVNEKGIIRNNTLSDYIIPTAMDVPHL
                     ETDVVNYPYIEGPFGGKGAGELPAVGGAPAYVSAMESALGKNLYKTPFSQEDAMKVLE
                     EIKK"
     gene            1364..>1825
                     /locus_tag="PKV59_09015"
     CDS             1364..>1825
                     /locus_tag="PKV59_09015"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQP46929.1"
                     /translation="MSNGISFILNGEKVVSKGKSGDRLLDILRDEFNLTGTKCGCKEG
                     ECGACSVLIDGKLANSCMVALGSIAGSEVVTIEGYSKTKRFQALSDAYAEFSAVQCGF
                     CIPGMVLASEALLSKNPHPSESEIREGISGNLCRCTGYNAIVHAIQTASEKG"
BASE COUNT          527 a          367 c          487 g          444 t
ORIGIN      
        1 aacgggaaga agatattatt tgcacctcta aacgccatcc cgccaccatg cgttatcggg
       61 cggcaatcaa ggataatcga attgcggcaa tacaagccga tgttgtttat aactgcggcg
      121 cctatacaac tctctctccg gtggttttgc aacgtggttt gatcggtgcg accggagttt
      181 acactttcga taatattcga gtcgaaggaa aaggtgtgaa aaccaatacc acgccaaacg
      241 gcgcatttcg cggtttcggc ggtccccaga catttttcgc aattgaaatg tttatgaatc
      301 atcttgcaaa agaagtggga agcgatccgt tgccgttcaa acgatcgtac ctttccaaac
      361 aaggtgatac aaccgccaca aacggaaaat atcatttcta tattccgatg ccggagatgt
      421 tggaaaaaat tctgaaagcc agtgattatg aacggaaata tgaaagttat aagcggcaaa
      481 ccggcagata tcggaaagga atcgggttgt ccatggtgta ccacggctgc gggtttacgg
      541 gaagcggcga acgtgacatg attaaagggg tcgtaaaact tcataaaaat gtcaataaca
      601 cggttgaaat cctgaccgcg ggaacggata tgggacaggg gttgaagacg actttcagca
      661 agatcgtggc aaaaaggctc gaaattccga ttgatcagat cattattcgg aacccggata
      721 ccgatcgggt tcccaattcc ggaccgaccg ccgccagtcg ctctttaatg gtagttggca
      781 ggctgttgga taaagccgcg ctcaaattaa aggaatgttg ggttgatgaa gaagaacagg
      841 aaataaccga acgttatgtc catccggatt ttgtgattcc gtgggatttg aatacatttc
      901 agggagatgc ttacccggat aatgcttggt cggtaaacgt tgttgaagtt gaagtcgaca
      961 ctttaaccgc ggtcagtaaa gtaatcggag cttggggcgt ttatgatgtc ggaacaccca
     1021 ttgacatggc aataatccag ggacagatgc agggcgggtt cctccaggga atcgggtatg
     1081 gttccatcga attgatggat gtcaatgaaa agggaattat ccgcaataat acattgagcg
     1141 actatattat tcccacggca atggatgtcc cccatttgga aacggatgtg gttaactatc
     1201 cttatatcga aggtccgttc ggcggaaaag gagcggggga gctccctgcg gtaggaggtg
     1261 cgccggctta tgtatcggca atggagagtg cgttgggaaa gaatctttat aaaactccat
     1321 tctcccagga agacgcaatg aaggtattgg aggaaataaa gaaatgagca acgggatttc
     1381 atttatctta aacggagaaa aagttgtatc caagggaaaa tccggtgatc gattgctgga
     1441 tatccttcgt gatgaattca atttaaccgg aacaaaatgc ggttgtaaag agggggaatg
     1501 cggagcttgt tccgtgttga tcgacgggaa actcgcaaat tcctgtatgg tggcgcttgg
     1561 aagcattgcc ggcagcgaag ttgtcaccat tgaagggtac agcaaaacaa agcgttttca
     1621 ggcattatcg gatgcctatg cggaattcag tgccgttcaa tgcggatttt gcatccccgg
     1681 aatggtttta gcttccgaag cattgctttc aaagaatccg catcccagcg aaagcgaaat
     1741 cagagaaggt atctcaggca atttgtgccg ctgtaccgga tacaacgcga ttgttcatgc
     1801 tattcaaaca gcatcggaaa aggga
//
LOCUS       DAOMIV010000453         1818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_74911_length_1818_cov_4.609189, whole genome shotgun sequence.
ACCESSION   DAOMIV010000453 DAOMIV010000000
VERSION     DAOMIV010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74911_length_1818_cov_4.609189"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PKV59_09020"
     CDS             complement(<1..988)
                     /locus_tag="PKV59_09020"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQP46930.1"
                     /translation="MSFRFDKKPDRRDFLKFVGLGAGALAFRPYYSETEFIPSGAKTV
                     RVAIANLSVYKEPWDESEILYQKFRDDLLNVYYEVNSEHGPGYNPIWYRVWGGYVHSG
                     HTQIVETRINEVDYSVNSTPLPGKIGEVTVPFTQSRLHNSNGSWEDVFRLYYQSVHWV
                     VGVQEGLDGRPWYRIKDEIFDADRLDYYVPAEHIRIVSDAEISPISPEVPWKEKRIEI
                     SIGMQQLIAYEYDRPVLKTKVSTGIPSRKIEGQISTSTPTGTFNIQDKMPSKHMGDGK
                     MTADLEAYELPGVPWVCFFEPLTGVATHGTYWHTNFSMTMSHGCVNMRNEEAK"
     gene            complement(990..1271)
                     /locus_tag="PKV59_09025"
     CDS             complement(990..1271)
                     /locus_tag="PKV59_09025"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HQP46931.1"
                     /translation="MTDHPQSEDSKGFTFPCEMPIRIIGENTEAFEEEVIEILSHYLP
                     EVAPIDFRTRMSRANNYCSIAYTFYAESREQVEGIYRELSLSKHVKWVL"
     gene            1378..>1818
                     /locus_tag="PKV59_09030"
     CDS             1378..>1818
                     /locus_tag="PKV59_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HQP46932.1"
                     /translation="MKNKLFILSFLFLFLRSFQPAVANCTNPPDKQHFQIFSESLNKN
                     IDFSVYVPPCYDKRLVKGYPVLYLLHGQNMDESVWDNLHLSEDLQAGWKDLTLPYFLV
                     ITIREEENLQDLFYSDFDRAILESVIPWVDQTYHTCTDPSCRAIA"
BASE COUNT          490 a          438 c          360 g          530 t
ORIGIN      
        1 atttggcttc ctcatttcgc atattgacac agccgtggct catagtcata ctaaaattgg
       61 tatgccaata tgttccgtga gtcgctacgc cggtaagcgg ttcaaaaaag catacccatg
      121 ggacaccggg aagttcatat gcttccagat cggcagtcat ttttccgtca cccatatgtt
      181 tggacggcat cttatcctga atattaaaag tgcctgtcgg tgtcgaggtt gaaatttgtc
      241 cttcaatttt tctggacgga atgccggttg aaacttttgt ctttaaaacc ggccgatcat
      301 attcataagc gatcaattgc tgcatcccga tcgaaatctc aatccttttt tctttccagg
      361 gaacttccgg agaaatcggt gatatttccg catccgaaac aatccgaata tgttccgccg
      421 gcacataata atccaaccga tcggcatcaa atatttcatc cttaatccga taccaaggcc
      481 ttccgtcaag cccctcctgt acaccgacta cccaatggac ggattgataa tataaccgga
      541 agacatcttc ccaacttccg ttgctgttgt gtaacctgga ttgggtaaac ggaacggtca
      601 cttcgccgat cttcccggga agcggcgtgg aattaacgga ataatccact tcattgattc
      661 gggtctccac aatttgggta tggccggaat gtacataacc gccccacacc cgataccaaa
      721 tgggattata gccggggcca tgttccgaat ttacttcata ataaacgttg agcaaatcgt
      781 ctctgaattt ttgatagaga atttcgcttt catcccacgg ttctttgtaa acactcaggt
      841 tggcaatcgc cacccgcacc gtcttcgctc ccgagggaat aaactccgtt tcgctgtaat
      901 acggacgaaa agcaagcgca ccggctccca atccgacaaa tttcagaaaa tcacgcctgt
      961 cgggcttttt atcaaatcga aaactcatgc tataaaaccc atttcacgtg cttgcttaag
     1021 gacagttcac gataaatccc ctcgacctgt tcacgggatt cggcataaaa agtataagcg
     1081 attgagcaat aattattggc gcgactcata cgggttctga aatcgatcgg ggctacctcc
     1141 ggcaaatagt gtgataaaat ctcgataacc tcttcttcaa aagcctctgt attttcgccg
     1201 ataattcgaa tcggcatctc acagggaaat gtaaaacctt ttgaatcttc tgattgtgga
     1261 tgatccgtca taaacaataa cctctttgtc gaagtatacc atgcagcaat tttgattgat
     1321 attgattaat gaaaccgcaa atgataggtc agtatggatg ggtataatta gtcgctgatg
     1381 aaaaataaat tattcattct ttctttttta tttcttttcc tgcgatcttt ccaacccgct
     1441 gtcgcgaatt gcacaaatcc accggataaa caacactttc aaattttttc cgaatcctta
     1501 aataaaaata tcgatttttc ggtttatgtt cccccctgtt acgataaacg ccttgtcaag
     1561 gggtatccgg tcctttatct gcttcacgga caaaatatgg atgaatccgt ctgggataat
     1621 ctgcatttgt ccgaggattt gcaagccgga tggaaggatc tgactctacc gtattttctg
     1681 gtcatcacaa ttcgggagga agaaaactta caggatcttt tttattccga ttttgatcgt
     1741 gcgattttgg aatctgtcat cccatgggtt gatcaaacgt atcacacctg caccgaccca
     1801 tcctgtcggg cgatcgcc
//
LOCUS       DAOMIV010000454         1817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_74977_length_1817_cov_3.348468, whole genome shotgun sequence.
ACCESSION   DAOMIV010000454 DAOMIV010000000
VERSION     DAOMIV010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74977_length_1817_cov_3.348468"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1451
                     /locus_tag="PKV59_09035"
     CDS             273..1451
                     /locus_tag="PKV59_09035"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQP46933.1"
                     /translation="MSITETPSKIEKILQDAAVNRLLPASQICIVRDSEVILSESFGY
                     VPVGSKKIKTQKLTLFDLASVTKLFTATAFMRLVDLNLISLEDAVSKILPEFSGERPI
                     APYEDPLNPGKLISVDHGFTGMVDAGEITFRQLLRHSSGLPAWRPLFTQPTVSAAKQM
                     VFDTFFSYKPDTDVVYSDLGFMLVGWAIEKICGMGLDQAIDHFVLKPLQLTETGFRPL
                     SRDFNTPLPLPAEGIAPTGICAVRHKILVGEVNDENCASLGGISGHAGLFATAEELAA
                     FGQSFLDESSILTSESMNEMKKVQAVSRDGLVRRGIGFQLWSADPNASYAALSPDSFG
                     HTGFTGTCLWVDPQRQMVIAFLTNEVSNGRKNRKILPLRAQILTEAAAICPVSADIPG
                     "
     gene            complement(1518..>1817)
                     /locus_tag="PKV59_09040"
     CDS             complement(1518..>1817)
                     /locus_tag="PKV59_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HQP46934.1"
                     /translation="TPIQPETGTVIPVQNDSENRIDLNPRLIMFGSTPTAEATEILVV
                     IGKNVGTEVAVTQSTALPKTGVADKWNIPMMILVVMVLLALILSVRLLRNKHSIK"
BASE COUNT          486 a          370 c          413 g          548 t
ORIGIN      
        1 gcaaatatga atttgttttt gatttcctaa gaggagatct gcgggagcaa aaccgtccca
       61 aaagtgaatc ggaaactgca atttcgaata agtttttaaa tatcagaata aatgtcacgt
      121 tataaagggg aaggttttta tttgacagaa ataaaatttt ctattacgct aaaaaacagt
      181 ctttaattaa actacttttg tttagtaaat ataatctttt atgttgatat ttttcggact
      241 gaagtatggc gattaagaaa gcgatgagat aattgagtat cactgaaact ccttcgaaaa
      301 ttgagaagat cctgcaagac gccgccgtaa acagattgtt acccgcttcg caaatttgta
      361 ttgttcgcga ctccgaagta atcctttccg aatctttcgg gtatgtgccg gtcggttcca
      421 aaaagataaa gactcagaag ctgactttat tcgatttggc ctcggtgaca aaactcttta
      481 ctgccacggc ttttatgcga ttggtagatt tgaacctcat ttctttggaa gatgcggttt
      541 caaaaatcct tccggaattc agcggggaac ggccgattgc tccatatgaa gatccgctca
      601 atccgggaaa gttaatatcg gtcgatcatg gttttacggg aatggtcgat gctggagaaa
      661 ttacgtttcg tcaactgctt cgccattcat cggggttacc cgcctggcgt ccgttgttta
      721 cccagcccac tgtttccgcc gcaaaacaaa tggtttttga tacttttttc agttataaac
      781 cggataccga tgtagtatat tccgatctcg gttttatgtt agttgggtgg gcaatcgaaa
      841 aaatttgcgg tatgggtctg gatcaggcga ttgatcattt tgttttgaaa ccgcttcaat
      901 tgactgaaac gggttttcgc cccttatcac gtgatttcaa tacaccctta ccgcttccgg
      961 cggaaggaat tgctccgacc ggtatttgtg cggttcggca taagatcttg gtcggcgaag
     1021 tgaatgatga gaattgtgcc agtttgggag gaatatccgg gcatgccggt ttgttcgcta
     1081 ccgcagaaga gctggctgct ttcggacaat ctttcttgga tgaatcttct atcctgacat
     1141 cggaaagcat gaatgaaatg aaaaaagttc aagccgtcag ccgggatggc ttggtccgac
     1201 gaggaatcgg ctttcaatta tggtcggctg atccgaatgc cagttatgcg gctttaagtc
     1261 cggattcttt tgggcataca ggttttaccg gaacatgctt atgggtcgat ccccagcgtc
     1321 aaatggttat tgcattctta acaaacgaag tttccaacgg aagaaagaac cgaaaaattc
     1381 ttccgttgcg agctcaaatt ctcactgaag cggcggcaat ttgtccggtt tccgccgata
     1441 ttcccggcta aaaaatcaat cgaattttcg aatcaaaaaa gccgatattt gtaatcggct
     1501 ttttttgatt tgtatttcta ttttatcgaa tgcttattcc taaggagtct gacactgaga
     1561 atgagcgcca aaagaaccat caccactaaa atcatcatcg gaatattcca tttgtcggcg
     1621 acacctgttt ttggcaaggc ggtggattga gttaccgcaa cttccgtccc gacatttttt
     1681 ccgataacga caagtatttc ggtagcttcg gcggtaggag tactcccaaa catgatcaac
     1741 cgcggattca aatcgattcg attttcagaa tcattttgaa ccggaatgac ggtgcccgtt
     1801 tccggttgaa tcggtgt
//
LOCUS       DAOMIV010000455         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_75537_length_1808_cov_2.933257, whole genome shotgun sequence.
ACCESSION   DAOMIV010000455 DAOMIV010000000
VERSION     DAOMIV010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75537_length_1808_cov_2.933257"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PKV59_09045"
     CDS             complement(<1..434)
                     /locus_tag="PKV59_09045"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQP46935.1"
                     /translation="MNKAKYYSSYLDINLDAIQHNCRICADKFRVPIMAVVKADAYGF
                     GALPVAKACAEAGVESFAVARIQEGIELREGGIRQDILVFSLQDEDEMRAATRYGLTI
                     SLNRFDQIEPIAKLKRESGISPKAHLKIDTGMSRFGFLPEELP"
     gene            841..>1808
                     /locus_tag="PKV59_09050"
     CDS             841..>1808
                     /locus_tag="PKV59_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46936.1"
                     /translation="MSRKVSILFVLTALLLAVVPVLAQDFQIPEIVEGKWNVAFVLIG
                     PINDGGWSQAHYDGAKYVSDNVDNVNVVYVENIPEGTESEQVFRSLAAKGFNVIFGTS
                     FGFMDPMEAVAEDFPDSYFIHIAGYKTNNTNFGNLMGAMEHMKFLAGYLSGQRCKMDG
                     EDTVGYMYTFPIPEELRLGNAFMMGVKKTCPECKMIVTPINSWHDPVVEQEAAKALFD
                     AGAYIVFTGADTPAPAIVAESYKEGKGKWGIPYDWKGSCTSKRCLTLPYWNWGPVYAS
                     IVEGLRDGTYTPGRHYFDGDEKGLGLYGFMEGEELTEGLKDMPEEVL"
BASE COUNT          522 a          378 c          390 g          518 t
ORIGIN      
        1 ggcaattctt ccggtaaaaa gccaaaacga ctcatccccg tatcaatttt taaatgcgcc
       61 ttcggagaaa tacccgattc tcttttgagt ttcgcaattg gttcaatctg atcgaaacgg
      121 ttgagagaaa tcgtcagccc gtaacgggta gcagcccgca tttcatcttc atcttggagg
      181 gagaatacca aaatatcctg ccgaatgccg ccttccctca attcgattcc ttcttgaatt
      241 ctggcgaccg caaaagattc aacacccgcc tcggcacaag ctttcgctac cggcaatgca
      301 ccgaatccat aagcatccgc ttttacaacc gccataatcg gaaccctgaa cttatctgcg
      361 caaattcgac agttatgttg gatggcatct aaattaatat caagatatga agaatagtat
      421 tttgccttgt tcatggaacg attcccccgc aatttattgt caataaggaa gcaactgttt
      481 ctatttcgtt aatacttaat taataagtat taattgtgca tacatatttc cccgacaaac
      541 tcctgaataa tttaatattt tatttgcaaa tagcatgttt aaaataagac gaattacaca
      601 taattggttt tattgtctga cattttattg ctttttttga ggtcatttta tataatcaga
      661 ttatgagact gaaatactat tcatggttta cgaggagaga attccgcttg atggaattct
      721 ctcattcagt catcttaagt tttgcggttt cggaacctaa atactttata atataaatag
      781 tgttgttcat acagtctcta tgttggaaga atcaaactat atttctgtaa aggagaaaaa
      841 atgtctcgaa aagtatctat attattcgta ttgacagctt tactgcttgc tgtcgtcccg
      901 gtacttgccc aggatttcca gattccggaa atcgtcgaag gcaaatggaa tgtcgcattt
      961 gttttgatcg gcccgatcaa tgatggaggt tggtcacaag cgcattatga cggtgccaaa
     1021 tacgtcagtg ataacgtcga caacgtaaat gtggtttatg tcgagaatat ccctgaagga
     1081 acagaatcgg aacaggtctt ccgcagcctt gcagccaaag gcttcaatgt gatctttggt
     1141 acatccttcg gatttatgga cccaatggaa gctgttgccg aagactttcc ggacagctat
     1201 tttattcata ttgccggcta taagaccaac aacaccaatt ttggtaatct gatgggtgcg
     1261 atggaacata tgaaattcct tgcgggatat ttgagtggcc aacgatgcaa gatggatgga
     1321 gaagacaccg tcggttatat gtatacattc ccgattccgg aagagcttcg cttagggaac
     1381 gctttcatga tgggcgtgaa aaaaacctgc ccagaatgca agatgattgt caccccgatc
     1441 aacagttggc atgatccggt tgttgaacag gaagccgcaa aagcattgtt tgatgccggt
     1501 gcctatattg tattcaccgg cgctgacaca ccggctccag caatcgttgc cgaatcctat
     1561 aaagagggaa aaggaaaatg gggaatccca tatgattgga aaggctcctg cacatccaaa
     1621 cgatgcctca ctctgccata ttggaactgg ggtccggttt atgcctccat tgtcgaaggg
     1681 ttacgcgacg gtacttatac cccgggacgc cactatttcg atggagatga gaaaggtcta
     1741 ggactctatg gattcatgga aggtgaggaa ttaacagaag gcttaaagga catgccggaa
     1801 gaagtact
//
LOCUS       DAOMIV010000456         1792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_76567_length_1792_cov_2.598158, whole genome shotgun sequence.
ACCESSION   DAOMIV010000456 DAOMIV010000000
VERSION     DAOMIV010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76567_length_1792_cov_2.598158"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PKV59_09055"
     CDS             complement(<1..633)
                     /locus_tag="PKV59_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQP46937.1"
                     /translation="MSTLKDVAVLANVSPITVSRVINTPDLVKPGTRLKVQKAMDQLQ
                     YSPNIPAKNLVTKRSGIINVYIPENMDLSNPFSMHFIAGVSQGLGENMYSFLLLRDRK
                     IETACDGYIVTGLEHREIYEFDDFARKRNRPIVLFGHTDIEAIDCIDIDNVAGAFMAT
                     EYLIRNGHRKIAMINVNENRDYVTDRLNGYRKALDAYDIPYRKKRVIYAEN"
     gene            complement(762..1370)
                     /locus_tag="PKV59_09060"
     CDS             complement(762..1370)
                     /locus_tag="PKV59_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HQP46938.1"
                     /translation="MDSRTDEMISGIALHWYSGDHFESLDLLQKDFPDKKLIISESCL
                     EYYKFGANLSDHSTQNLAHDLAGNMNHGLNAFYDWNLLLDQHGGPNHAGNYCDAPMLY
                     DTSSKQMNKRPSMVYYWHFSHFIHPGAVRIAFSRFTDDIDVTAWKNPDKSIAIVLLNR
                     NGVSTACNIRIHEKITKLTLPPYAIGTLVLRSGVSDCSPELL"
     gene            complement(1370..>1792)
                     /locus_tag="PKV59_09065"
     CDS             complement(1370..>1792)
                     /locus_tag="PKV59_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46939.1"
                     /translation="SFSFARTEKYIVPMLKAAQQTARRPLKLMLTPWTPPAFMKTNHD
                     RCHGGCLKPEYRSMWAKYICRYITEFRMRGWQVNRISIQNEPHAIQKWESCLFSAEEE
                     RTFLRDFLYPELLKQNLADIEIYIGITTKNGRMSGLKR"
BASE COUNT          528 a          385 c          337 g          542 t
ORIGIN      
        1 attttccgca taaatcactc ttttttttcg atatggaata tcatatgcat caagcgcctt
       61 acgatagccg tttaaacgat cagtcacata atcgcgattc tcgttgacat ttatcatggc
      121 aattttacga tgaccattcc gtattaaata ttcggttgcc ataaaagcac ctgccacatt
      181 atcaatatcg atgcagtcta ttgcttcgat gtcggtatga ccgaaaagaa caattggccg
      241 gtttcttttg cgtgcaaaat cgtcgaattc atatatttct cgatgctcta accctgtcac
      301 gatatatccg tcgcatgcgg tttcaatctt tcggtcacgc aataaaagaa aagaatacat
      361 attctcccct aagccctgac tgaccccggc aataaagtgc atcgagaacg gattacttaa
      421 atccatgttt tccggaatat atacattaat aattccggat cgtttggtaa cgagattctt
      481 tgcgggaatg tttggcgaat attgcagttg atccatcgct ttttgaacct tcaagcgggt
      541 gcccggcttc accaaatccg gcgtatttat aacacgggat acggtgatcg gagaaacatt
      601 ggccagcact gcgacatctt ttaaagtcga catattaatc catcctcgct aatgataacg
      661 ctatcatttt ttatagtaaa aatttttgtt cgatcttgct taatgataac gctatcattt
      721 ttatatcaca caattcttgg ttttgcaata cctgaaaata cttacaataa ttcaggactg
      781 caatccgata ccccgctccg taaaaccaga gtgccgattg catagggagg aagcgttagt
      841 tttgtaattt tctcgtgaat tcgaatgtta caagcagttg atacaccatt gcgatttaat
      901 aacacaatcg caatcgattt gtcgggattt ttccaagcgg taacgtcaat atcatctgta
      961 aatcgggaaa atgcaattcg gacagcgccg ggatgaataa aatgagaaaa atgccaataa
     1021 tagaccatag acggtcgttt gttcatttgc tttgatgagg tatcatacaa catgggagca
     1081 tcacaataat tccccgcatg attaggccct ccatgttgat ctaacaataa attccaatca
     1141 tagaaagcat ttaacccatg gttcatatta ccggccaggt catgggcaag attttgagtg
     1201 gaatgatcag aaagatttgc cccaaactta taatattcga gacaactttc cgaaataatc
     1261 aatttcttat ctggaaaatc tttttgcaat aaatccaatg attcaaaatg atcaccactg
     1321 taccaatgaa gggcgatacc gctgatcatc tcatctgtcc gagagtcaat taccgcttta
     1381 gcccgctcat acgcccgttc tttgttgtga tcccaatata tatttcgata tccgcaagat
     1441 tttgcttgag aagttccgga tacagaaaat cacggagaaa agttctttct tcttcagccg
     1501 agaacaagca cgattcccat ttttgaatgg catgcggttc attctgaatc gaaatccggt
     1561 ttacttgcca acctcgcatc cgaaattcgg ttatatagcg acaaatatat ttagcccaca
     1621 ttgaacgata ttccggtttc aaacagcctc catgacaacg atcatgattt gtcttcataa
     1681 atgccggcgg tgtccaaggt gtcaacatca gtttcaaagg tcggcgagcg gtttgttggg
     1741 ctgcttttaa catagggacg atatattttt cggttctggc gaacgaaaat ga
//
LOCUS       DAOMIV010000457         1787 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_76893_length_1787_cov_3.465358, whole genome shotgun sequence.
ACCESSION   DAOMIV010000457 DAOMIV010000000
VERSION     DAOMIV010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76893_length_1787_cov_3.465358"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1668
                     /locus_tag="PKV59_09070"
     CDS             172..1668
                     /locus_tag="PKV59_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46940.1"
                     /translation="MSKKKNSERSGNISPRKEAFDTAWEKFEPLIPEEELPALKACIQ
                     FTPDPAIRLNPQKASISDIGKLRERYHWDTEPVAFCPDGYKLKDTHGIAPGQTNEHRC
                     GAFYIQDSASMLPVTLFDQQKILNKPLILDMAASPGGKTTHLSAASRDSGLILANDSS
                     ASRITILKKVMKNWGSIHHLVTVFPGEFFGQWYPNTFDLVLLDAPCSMQSLVSIASHP
                     MRPISEREENALAQRQLSLLDSALKAVKPGGQVVYSTCTLSPAEDELVINGILKKYGA
                     QITLLDAQQKLPYPAPGLVSVEGSEPNPSLERTIRLWPHRYQTTGFFAALIQKNDTFG
                     FSEQIEKPFRRSREELRFIEIDPRQDHHPIERMNEIFGFNLTEFAEQRECCFRMRGNE
                     LWAVPTVYFDEFVDWPPCKSCGMRIAAQTASGWLPDFDWVSMIFDCITKNHYPLDETL
                     TEAWVRGEDLPISLPEYSKGTILFMTDSWNVFIGCGYVSGNRIRNLTK"
BASE COUNT          495 a          429 c          424 g          439 t
ORIGIN      
        1 aacaactttc ggtaaaatgg attgtggtca ctctgccggc agaaaacctg accggccggc
       61 atcaaatgcg tgatcgcgaa cgtatgctga tagagaaaac aatcaccgat ttggattggg
      121 aaatcagcga aacggaagtg gggacggaaa tgattttctt tatcaggaaa tatgagtaaa
      181 aaaaagaatt cggaaagaag cggaaacatc tcccctcgaa aagaggcttt tgacaccgcc
      241 tgggaaaaat tcgaaccgct gattcccgaa gaggaattgc cggcattaaa ggcatgtatt
      301 caatttacgc cggacccggc gattcgatta aacccgcaaa aagcaagcat atcggatatc
      361 ggaaaattac gggagcgata ccattgggac accgaaccgg tcgctttttg tcccgacggt
      421 tataaattaa aagataccca cggaatcgca cccggccaaa caaatgaaca tcgctgcgga
      481 gcattctata ttcaggacag cgcctccatg ttgccggtta cactttttga tcagcaaaag
      541 attctgaaca agccgttgat tttggacatg gcagcttcgc ccggcggcaa aacaacccat
      601 ctttccgcgg caagccggga ttcgggactg attcttgcca acgattccag cgcctcccgc
      661 atcacgatat tgaaaaaagt gatgaaaaat tgggggagta ttcatcatct ggtcaccgtt
      721 tttcccggtg aatttttcgg acaatggtat ccgaatacat ttgatctggt gttattggat
      781 gcgccctgca gcatgcagag tttggtttcc atcgcttctc atccgatgcg cccgatcagc
      841 gaacgggaag aaaacgcact ggcgcaacgc cagctgtcgc tgctcgacag cgccttgaaa
      901 gccgtcaaac ccggcggtca ggttgtttat tcaacctgca ccctttcacc ggcggaagat
      961 gaattggtga tcaacgggat tctgaaaaaa tacggtgcgc aaatcacttt gcttgacgct
     1021 caacagaagc tcccatatcc ggcgccgggt ctggtatcgg ttgaaggatc agagccgaat
     1081 ccgtctttgg aaagaacaat ccggttgtgg ccgcatcgtt atcagaccac cggatttttt
     1141 gccgcattga ttcaaaagaa tgatactttc ggattttcgg aacaaataga aaaacccttc
     1201 cgtcgatcac gcgaagaact tcgattcatt gaaatcgatc ccagacaaga tcatcatccg
     1261 atcgagcgca tgaacgagat tttcggattc aatcttaccg agttcgcgga acaaagagaa
     1321 tgttgttttc ggatgcgcgg caatgaactt tgggctgtcc caaccgtata ttttgacgaa
     1381 ttcgtcgatt ggccgccttg caaatcctgc ggtatgcgca tcgccgctca gacggcctcc
     1441 ggatggctcc cagattttga ttgggtcagc atgatctttg attgtattac caaaaaccac
     1501 tacccattgg acgaaacgct gacggaggcg tgggttcggg gagaagattt gcccatatcc
     1561 ctgcctgaat attccaaggg aacaatcctt ttcatgacgg attcctggaa tgtattcatt
     1621 ggttgcggct atgtcagcgg gaatcggatc cgcaacctga caaaataaaa gacagagatt
     1681 ttttctctgt ctttttctta atctaaatcc cagtaaaaac gatctcttct ataaaacatt
     1741 ccatggattt acgtatccac ctccgtatcg cacttcgaaa tgaaggt
//
LOCUS       DAOMIV010000458         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_77170_length_1783_cov_2.950231, whole genome shotgun sequence.
ACCESSION   DAOMIV010000458 DAOMIV010000000
VERSION     DAOMIV010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77170_length_1783_cov_2.950231"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PKV59_09075"
     CDS             <1..573
                     /locus_tag="PKV59_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46941.1"
                     /translation="KTPRFPSVSIPLLADIPFIGPIVFYNSLFVYGMILLMIILQFAL
                     FRTRWGLRLRSVGEHPKAADTLGINVYKTQYTAVILGGLMAGFAGAYFSLGSVGRFDE
                     GMTAGKGYIGLAAMIFGGWNPFNAMMAGILFGFADAMASSVSILGSSIPGQFINMLPY
                     VVTMIVLAGLVGGHSTGPAASGVPYEKEST"
     gene            complement(700..1041)
                     /locus_tag="PKV59_09080"
     CDS             complement(700..1041)
                     /locus_tag="PKV59_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HQP46942.1"
                     /translation="MIDFDNRPWGRWEGYIFEPNYRVKRIIVFPGKRLSLQLHHLRSE
                     IWVIVQGSGKMTLGDKIFPIHEGDIVQIPKGQAHRVENDSDQPLVLIETQLGICPEDD
                     IVRLEDDFGRV"
     gene            complement(1052..>1783)
                     /locus_tag="PKV59_09085"
     CDS             complement(1052..>1783)
                     /locus_tag="PKV59_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQP46943.1"
                     /translation="AGENGQLQATFDLLNIHYTGTGFAGCVLAMDKHLAKEVMVANDL
                     PTAPWVYLDRYDQLNWDQIDSLGYPCVIKPPRGGSSIGVSIPQNKEEFLRGIQAAIAS
                     AEDRQPDLLVEKYLAGREFCIGILEGISLPAIEIIPNEGWFDYEKKYQSGLTREVCPA
                     EIPEELSIRLQDTALKIHRALKLGYYSRIDFKVDGDGNIYCLEANTLPGMTPTSLFPQ
                     EAAAVGIGYADLCDKIVRNTERKEE"
BASE COUNT          428 a          439 c          411 g          505 t
ORIGIN      
        1 aaaacaccgc ggtttccgag tgtctccatc ccgttgcttg ccgatattcc ctttatcggt
       61 ccgattgttt tttataacag cctttttgtt tacgggatga ttttgttgat gattattctg
      121 caatttgctc tctttcgaac ccggtgggga cttcgtctgc gatcggtggg tgaacatccg
      181 aaagctgcgg atacattggg cataaatgtc tataaaaccc aatatacggc tgttattttg
      241 ggtggtttaa tggcgggatt tgccggcgct tacttttcat tgggttcggt gggcaggttt
      301 gacgaaggca tgacagcggg caaaggatac atcggcttag ctgcgatgat cttcggcggt
      361 tggaacccct ttaatgcgat gatggccgga atattgttcg gatttgcgga tgccatggca
      421 tcatcggtct cgatcttggg atcttccatc ccggggcaat tcattaatat gcttccgtat
      481 gtcgttacga tgattgtgtt agccggattg gtcggcgggc acagcaccgg accggctgcc
      541 agcggcgtcc cttatgaaaa ggaaagcact tgatttacat gaatcattcg atatattaaa
      601 cagaaacagg agaaaacccg aaaagttttc tcctgttttt atatgctgtc gatcaaagaa
      661 cgatcagtct gccgagaaaa ggcagatata gttgaagagc taaacccgtc cgaagtcatc
      721 ctcgagccgg acaatatcat cctccggaca gatccctaat tgcgtttcga tcaaaaccaa
      781 aggttgatcc gagtcgtttt caacccgatg tgcctgccct tttgggattt ggacgatatc
      841 tccctcgtga atcgggaata ttttatcccc caaggtcatt tttccggaac cctgaacgat
      901 gacccatatt tcactcctca gatgatgaag ttgcaaagat aatcgtttcc cggggaagac
      961 gataatcctt ttcacccgat aattcggttc gaaaatatat ccttcccagc gaccccaagg
     1021 cctgttatcg aaatcgatca ttctttctcc tttattcttc cttgcgctct gtatttctta
     1081 caattttatc gcacaagtcc gcataaccga taccgactgc cgcggcttcc tgtggaaaca
     1141 ggctggtcgg tgtcattccc ggcaatgtat ttgcttccaa acaatagata tttccgtctc
     1201 cgtccacttt aaaatcgatc cgggagtaat aaccgagctt taacgcccgg tgaattttca
     1261 acgccgtatc ctgtaaacgg attgataact cttccggaat ttccgccgga cagacttccc
     1321 gcgttaatcc ggattgatac tttttttcat aatcaaacca accttcgttt gggatgattt
     1381 cgattgccgg tagggaaatg ccctcgagaa ttccgataca aaactcccgc ccggctaagt
     1441 acttttccac aagtaaatcc ggttgacggt cctcggcgga ggcgatggcg gcttgaattc
     1501 cgcgtaaaaa ttcctctttg ttttgcggaa tactcactcc gatcgacgaa ccaccacggg
     1561 gaggttttat cacgcaggga tatcccagag aatcgatttg atcccaattc agttgatcat
     1621 atcgatccag ataaacccag ggagcggtgg gaaggtcgtt agccaccatt acttccttcg
     1681 ccaaatgttt atccatggca aggacgcagc ccgcaaaacc cgtaccggta taatggatat
     1741 tcagcagatc gaacgttgcc tgcagctggc cgttttcgcc ggc
//
LOCUS       DAOMIV010000459         1781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_77301_length_1781_cov_3.679606, whole genome shotgun sequence.
ACCESSION   DAOMIV010000459 DAOMIV010000000
VERSION     DAOMIV010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77301_length_1781_cov_3.679606"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1030)
                     /locus_tag="PKV59_09090"
     CDS             complement(50..1030)
                     /locus_tag="PKV59_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQP46944.1"
                     /translation="MTKPEYALALDFGGTKLATAVVSVPDGRLFGYQKARTPADAGAQ
                     ASLELILNTAGKSLKESGIPQEKITRGGISFGGPVNPDGKHVIRSMHIKGWENYPLAE
                     VAADRFGFPFRMGNDGNVAALGEWFYGSQGKPHYFMYIQISTGIGGGFILNGEILQGQ
                     GMAGEVGHQKVQYVGEAAFPCACGSKGCIEAVASGWALARDAKALIESPEACSVFREQ
                     VSKMGEISAKPLIEAARRGDSSASEIVDYAFRCFALSLSNAIVLFDLEEIVIGGGVAV
                     NSWDLLEPILTHSIPEFLPTEMRGKTKIRMSHLKGTETLLGAAKLSELAG"
     gene            complement(1027..1620)
                     /locus_tag="PKV59_09095"
     CDS             complement(1027..1620)
                     /locus_tag="PKV59_09095"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQP46945.1"
                     /translation="MNSTKQEWVNEYIGLLNQAIDKMPVDQIVRAAEMIHSARLERKM
                     IFTMGNGGSSATASHMACDFSKNTRVPYLPAIRTMCLSDSTPTLTAFANDEGYEKVFS
                     EPLKALAEPGDILIAISGSGNSANILRGLETAREIGLKTIGLSGFKGGQMISMVDLPI
                     NIDTDSIERVEDLHMVCDHLLTMVLREIAMHEPEGKR"
BASE COUNT          416 a          480 c          391 g          494 t
ORIGIN      
        1 gaagggattg ttcggcgcgc atttgaattc ttcggcgaat aagtcttttt cagccggcaa
       61 gttcggataa ttttgcggct cccaacaaag tttcggtccc tttgaggtga gacattcgaa
      121 ttttggtctt ccctcgcatt tcggtgggga gaaattcggg tatagagtga gtcaaaattg
      181 gttccagtaa atcccagcta ttcaccgcca ctccgccgcc gatgacaatc tcttccaaat
      241 cgaataaaac aatggcattc gaaagcgata aggcaaaaca tctgaacgca taatcgacga
      301 tttcggatgc ggaagagtct cctcttctgg ccgcttcgat caacggcttt gccgatattt
      361 cgcccatctt cgagacttgt tcacgaaaca cagagcatgc ttccggactt tcgatcaacg
      421 ctttggcatc ccgtgccaaa gcccagccgg atgcaacagc ttcgatacat cctttgctgc
      481 cgcaggcaca ggggaacgcg gcttcaccca catactgcac tttctggtga ccgacttctc
      541 cggccattcc ctgtccttga aggatttctc cgttcaagat gaagccaccc ccgattccgg
      601 tgctgatctg aatatacatg aaataatggg gttttccttg gctgccatag aaccattcgc
      661 ccaatgcagc cacattcccg tcatttccca ttcgaaaagg gaacccgaac cggtcggcgg
      721 caacttccgc cagcgggtaa ttctcccagc ctttaatgtg catagaccgg attacatgct
      781 tgccgtcggg atttaccggt ccgccgaaac tgattcctcc tcgggtaatt ttttcctgcg
      841 gaataccgga ttcttttaga ctcttacccg ccgtattcag aatcagctct aaactggctt
      901 gtgcacccgc atccgcgggc gtcctcgcct tttggtaacc aaacagcctg ccgtcgggaa
      961 cagagactac cgcggttgcc agtttggtcc cgccaaagtc gagcgccaga gcatattcgg
     1021 gttttgtcat cgctttcctt ccggttcgtg catcgctatt tcacgcagga ccatggtcag
     1081 taaatgatca cataccatat gtaagtcttc gacccgctca atgctatccg tatcaatgtt
     1141 gatcggtaaa tccaccatgg agatcatttg tcctcccttg aatccggata aaccgattgt
     1201 ttttaagccg atttctcttg cggtttccaa accgcgaagg atattggcag agttaccgct
     1261 tccgctgatg gcaatcaaaa tatcgcccgg ctcggccaga gctttcaacg gttctgaaaa
     1321 tactttttca taaccttcat catttgcaaa agccgttaaa gtcggagtgc tatcgctgag
     1381 acacattgtg cgaatcgccg gaaggtatgg aacacgagtg tttttcgaaa aatcgcacgc
     1441 catatggctg gcggttgccg aactgccgcc gtttcccatc gtaaaaatca tcttccgttc
     1501 caatcgagcc gaatgaatca tttcggcggc tcgtacgatt tgatcgaccg gcattttatc
     1561 aatcgcctga ttcaaaaggc caatatattc attgacccat tcttgttttg tgctgttcat
     1621 aattatttct catcctctta ttgacatcaa aatttcattc ggctttccgg tttccgggag
     1681 cggatttatt cctgcccgaa atgccgctcc aacattttgc ataaggtatg atagatcaga
     1741 atatgcaggt tctgaatttc atccgtctgc gattgattca c
//
LOCUS       DAOMIV010000460         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_77653_length_1776_cov_4.489831, whole genome shotgun sequence.
ACCESSION   DAOMIV010000460 DAOMIV010000000
VERSION     DAOMIV010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77653_length_1776_cov_4.489831"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..>1776
                     /locus_tag="PKV59_09100"
     CDS             92..>1776
                     /locus_tag="PKV59_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQP46946.1"
                     /translation="MKFLKTLHARPSDIARFRQEYDLIKNLDIKGIIKTFDVLHYKEG
                     FLLCQEDFAGIPIKNYLDKKESFDLKSFLEISAHIAETLGNIHNHGIIHRAIKPHNVL
                     INPKTEDIKITNFGISTIITHEHDEAYNPDFIRGSLAYMSPEQTGRMNRDVDYRTDFY
                     SFGITLYEILTGILPFQTNDPMELIHAHIAIMPEPPDKINSKIPGVVADMIMRLLAKN
                     PEARYQNGFGLAADFRTCIKQLEKNKKIESFELGKTDISNRFIIPQKLFGREKEIEKL
                     IASFEEVANREKGVSVMVVTGAPGIGKSVLVNEIHKPIVAKRGYFISGKYEQFRRDKP
                     YSAIIQAFQMLVKQLLSESGDRITLWKDNLLKALGDNGKVITDVIPEVELIIGKQPDL
                     PSLGPEESRNRFNFVFEKFIKVFPQKEHPVTLFLDDLQWADLASLQLMKLMMSGDIQH
                     LFLIFSYRDNEVDELHPVTDLLKDALKNKIKVDEIMLGPLKQKEVTDLIVHFLRCSEE
                     RGFGLAELVQKNTGGNPFFVNQFLHTLYHEKMIIHKGAEGWQWDIGEISRMKVA"
BASE COUNT          561 a          369 c          390 g          456 t
ORIGIN      
        1 cggcgattat atacttcaaa agaaaattca cgagaccaga aattccatta cgtatcgtgg
       61 gcataaaaaa aatgaatctc aatcgttcat catcaaattc ttgaaaaccc tccatgcaag
      121 accgtcggac atcgcccgtt tccgtcagga atatgatctg attaaaaatc ttgatatcaa
      181 aggcatcatc aaaacctttg atgttcttca ttataaagaa gggtttttgt tgtgccagga
      241 agattttgcc ggtatcccca taaaaaacta cctggataaa aaagaaagct ttgatttaaa
      301 gtcatttttg gaaatttctg cccatattgc cgagacgctt ggaaacatcc acaaccatgg
      361 aatcattcac agagcgataa aaccgcacaa cgttttaatc aatccgaaaa cggaagatat
      421 taaaataacg aatttcggta tatcaacgat cattacccat gaacatgacg aagcttataa
      481 tcctgatttc atcagaggct ctttagccta tatgtcgcct gaacagaccg gaagaatgaa
      541 ccgggatgtt gattacagga cagattttta ttcattcgga atcacgctct atgagatcct
      601 gacaggaatt cttcctttcc agaccaatga tccgatggaa ctcattcatg cacatattgc
      661 tatcatgccg gaaccgcccg ataaaattaa ttccaaaatc ccgggcgttg tcgcggatat
      721 gattatgaga ctgctcgcca aaaatccgga ggcacgttac cagaacggct tcggcctggc
      781 agccgatttc agaacatgca tcaaacaact ggaaaagaac aaaaagatag aatcgtttga
      841 actggggaag actgatatat ccaacagatt catcataccg caaaaacttt ttggtcggga
      901 aaaagaaata gaaaaattga tagcaagttt cgaagaagtc gccaaccggg aaaagggcgt
      961 ttccgtgatg gttgtgaccg gagcgccggg catcggcaaa tcggtactgg ttaatgaaat
     1021 tcataagccg atcgtggcaa aacgcggata ctttatttcc ggcaagtatg agcaattcag
     1081 aagagacaag ccctacagcg caatcatcca ggctttccag atgctggtca agcaactgtt
     1141 gtcggaaagc ggtgacagaa taacgctttg gaaagataat cttttaaaag cgttgggtga
     1201 caatggaaaa gtcatcaccg atgtgattcc tgaagtagaa ctgatcatcg gaaagcagcc
     1261 ggatttgcct tctctcggtc ccgaggaatc cagaaacaga tttaattttg tttttgaaaa
     1321 attcataaaa gtatttccgc aaaaggagca tccggttact ctttttcttg acgatctgca
     1381 atgggcggat ctggccagcc ttcagctcat gaagctgatg atgtccggcg atatacagca
     1441 tctctttctg atattttctt acagggacaa cgaggttgat gagctgcatc ccgtgaccga
     1501 tctgcttaag gacgccctga agaacaaaat aaaagttgac gaaataatgc tcggcccttt
     1561 gaagcagaaa gaggtcacgg atctcatcgt tcattttttg cggtgttcgg aagaaagggg
     1621 gtttggcctt gccgaactgg tacaaaaaaa tacaggcggc aatcccttct ttgtcaatca
     1681 gtttcttcat actctttatc atgaaaagat gattattcat aaaggcgcgg agggctggca
     1741 gtgggatatt ggtgaaatca gccggatgaa agtggc
//
LOCUS       DAOMIV010000461         1776 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_77655_length_1776_cov_4.112144, whole genome shotgun sequence.
ACCESSION   DAOMIV010000461 DAOMIV010000000
VERSION     DAOMIV010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77655_length_1776_cov_4.112144"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /gene="ygeW"
                     /locus_tag="PKV59_09105"
     CDS             complement(<1..1074)
                     /gene="ygeW"
                     /locus_tag="PKV59_09105"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HQP46947.1"
                     /translation="MKKSKKLLEELKKLDFHKMYNEDFLLTWEKSDDEIAATFKVADI
                     LRDIRENNISPRLFDSGLAVSLFRDNSTRTRFSYASAANLLGLEVQDLDEGKSQVAHG
                     ETVRETANMISFMADVIGIRDDMYIGKGNAYMREVSKAVEQGYKDGILEQRPTLVNLQ
                     CDIDHPTQSMSDMLHIINTFGGVENLKGKKIAMSWAYSPSYGKPLSVPQGIIGLMTRF
                     GMEVVLAHPQGYEVMPEVEKVAKKNAKASGGSFKVTNSMAEAFENADIVYPKSWAPFN
                     AMEKRTELYGQGDFDGIKALEKELLAQNAEHKDWECTESLMKTTANGNALYMHCLPAD
                     ISGVSCKEGEVESSVFDRYRTPLY"
     gene            complement(1111..>1776)
                     /locus_tag="PKV59_09110"
     CDS             complement(1111..>1776)
                     /locus_tag="PKV59_09110"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HQP46948.1"
                     /translation="RGRMEIKVHTHGISCHGSAPERGDNAIYKMAQIIEEVRALNENL
                     HYDPFLGKGTVTISEIFCTTPSRCAVADGCTISLDRRLTNGETWEGALQEVRNLPGVK
                     MADAEVSLYTYERPSWTGLTYPTECYFPTWVIPEDHPTTKTLVEAYEGLYEKEPVVDK
                     WTFSTNGVAIMGRYGIPCIGFGPGAEEEAHAPNEKTWTSHLVQAAAMYAAVPKLYVNK
                     YAK"
BASE COUNT          452 a          471 c          349 g          504 t
ORIGIN      
        1 ataaagagga gtccgataac gatcaaacac cgaactttcc acttcacctt ccttacagct
       61 caccccagaa atatcagccg gcagacagtg catatataag gcatttccgt tggcggtagt
      121 cttcatcaga ctttctgtgc attcccaatc cttatgctcg gcattttgtg ccaacagttc
      181 tttttccagc gctttaattc cgtcaaaatc accttgcccg tacagctcgg tgcgtttttc
      241 catcgcattg aaaggtgccc agctcttcgg atagacgata tccgcatttt cgaaagcttc
      301 cgccatcgag ttggtcactt taaagctgcc cccgctcgct ttggcatttt tcttcgcaac
      361 cttttccact tcgggcatca cttcataacc ctgcggatga gccagaacga cttccatgcc
      421 gaaacgggtc attaacccga taattccctg aggaaccgaa agcggcttcc cgtagctcgg
      481 tgaataagcc caagacatgg caattttctt tccctttaaa ttttcgactc ctccgaaagt
      541 attaataata tgtaacatat ccgacatact ttgagtcgga tgatcgatat cgcactgcaa
      601 attgaccaac gtcggccgtt gttccaaaat accgtcttta tatccctgct cgacagcctt
      661 gctgacttca cgcatatagg catttccctt gccgatatac atgtcatccc ggatcccgat
      721 tacgtcagcc ataaaggaaa tcatattggc tgtttcccgc accgtttctc catgagcgac
      781 ctgcgatttt ccttcatcta aatcctgaac ttcaagtccc agtaaatttg cggcgctggc
      841 ataactgaaa cgagtacggg tggagttgtc acggaataaa gaaacggcca atccgctgtc
      901 aaataatcga ggggagatat tattttcgcg tatatcgcgc aaaatatccg caactttaaa
      961 agttgccgca atttcatcat ccgatttttc ccatgtcagc aaaaaatctt cgttatacat
     1021 cttgtgaaaa tcaagctttt tcagctcttc cagtaatttt tttgactttt tcattgatca
     1081 ttatcctccg aataacaaat aatcaaaatt ttattttgca tacttattga cgtatagctt
     1141 cgggaccgcc gcatacatcg ccgccgcctg aaccaaatga ctggtccaag ttttttcatt
     1201 cggtgcatga gcttcctctt ccgcccccgg gccaaatccg atacagggaa tgccgtaacg
     1261 tcccataatt gcgacaccgt ttgtcgaaaa cgtccattta tcgaccaccg gttccttctc
     1321 atataatcct tcgtaagctt caacgagagt tttggtggtc ggatggtctt cgggaatcac
     1381 ccacgttggg aaataacatt cggtcggata ggtcaaaccg gtccaagacg gccgttcata
     1441 agtatataac gaaacttccg catcagccat ttttacgccc ggaagatttc gaacttcttg
     1501 gagtgcgcct tcccaagttt ccccgttggt caacctgcga tcgagagaaa tagtacagcc
     1561 atccgcaacc gcgcaacgtg agggggtcgt gcaaaagatt tcggaaatgg taaccgtccc
     1621 ttttcccaag aagggatcat agtgtaaatt ctcgttgagc gctcggactt cttcaataat
     1681 ctgagccatt ttatagatcg cattatcacc gcgttcgggt gcggaaccgt gacagctaat
     1741 accatgagta tgaactttta tttccattcg accgcg
//
LOCUS       DAOMIV010000462         1771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_77998_length_1771_cov_3.795455, whole genome shotgun sequence.
ACCESSION   DAOMIV010000462 DAOMIV010000000
VERSION     DAOMIV010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77998_length_1771_cov_3.795455"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..971)
                     /locus_tag="PKV59_09115"
     CDS             complement(129..971)
                     /locus_tag="PKV59_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HQP46949.1"
                     /translation="MDVIRPGSLQDCLRILKTEHTIPIAGGTDLMVNLALNKISPDRI
                     IDITGLQELKEISLSDNFLKIGALVTFSRLVGKEIAQYPCCNALREAAEKMGSPQIRN
                     RATIGGNLVTGSSAGDGSVAILALDAQVELTGLDGIRKVPLSLFYSGYHQTAIHSDEL
                     LTAVYIPIRPGTSSFQKVGKRNALAIAVASLAIYSYIRDSHFEQIYIAIGSVAPTPIR
                     AVKTDLFLQGQKITGGVIKEAARIIQDEIAPITDIRGTADYRRKLVANLLTDQLQQLS
                     ESKN"
     gene            complement(971..>1771)
                     /locus_tag="PKV59_09120"
     CDS             complement(971..>1771)
                     /locus_tag="PKV59_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQP46950.1"
                     /translation="KGVPDSAKVEIDREPNGDFTVRVGLVDIGQGNATALAAIAGEAL
                     KVPLEKIRLIMADTAQTFDCGSTAGSRSVFIAGNAILAAVRDYFSHPETGRGFAETEF
                     PQSKTDLNVIGFPHAMYTFIAQAVKLKLDPISGQPQLAGIFAATEAGKVINRLSMDGQ
                     IQGGIAMSIGYTLGENMNYRNGIPDNQRFTVYILPTAEDIPPIQNVSVELYEATGPYG
                     AKGAAEVATVVIAPAITSAIESIRDLKINRLPLDFEQILDAKEKGSVC"
BASE COUNT          460 a          425 c          389 g          497 t
ORIGIN      
        1 tgcacccttc tttaacaccg gttagattca tttcctcacg cagaaaatca attaaacgca
       61 tatcggatgg tatttcggaa tggatttctt cgccgtttat tttacaggaa agcatcactt
      121 tactcatctc aatttttaga ctccgacagt tgttgtaatt gatccgttaa aagattagca
      181 accagctttc ttcgataatc ggcggttccc cggatatcgg taatcggtgc gatttcatcc
      241 tgaatgattc gtgccgcttc tttaatgact ccgccggtaa ttttttgacc ctgaagaaaa
      301 agatctgttt tgacagcccg aatcggggtc ggagccacag agccgattgc gatgtaaatc
      361 tgttcgaaat gcgaatcacg tatatatgaa taaatagcca aactggcaac cgcaattgca
      421 agtgcattgc gtttcccgac tttttgaaag gatgaagtcc cgggacggat gggaatatat
      481 accgcggtca gcagctcatc agaatggata gcggtctgat gataaccgct gtaaaaaaga
      541 gataacggaa cttttcgaat tccgtccaaa ccggttaatt ctacctgggc atccaacgca
      601 aggattgcca ctgaaccgtc accggccgat gaacccgtga ccaaattccc gccgatcgtt
      661 gctcggttgc gaatttgcgg tgaccccatt ttttctgcag cttctcgtag agcgttgcag
      721 catgggtatt gtgcgatttc ctttcccaca agtctcgaaa aagtgaccaa tgcgcctatt
      781 ttcagaaaat tatctgaaag agatatttct ttcaattctt gtaacccagt gatatcaatg
      841 attctatccg gagaaatttt attcaacgcc aggtttacca tcaggtcagt tccgcccgca
      901 atgggtatgg tatgttcggt tttcaagatc cgtaaacagt cctgcaagct gccggggcga
      961 attacatcca tcagcaaacc gagcctttct ctttcgcatc cagaatttgt tcgaaatcta
     1021 ggggtaaccg attgattttt aaatcccgaa tcgattctat agcggatgtt attgccggtg
     1081 cgatgacaac tgtcgcaact tcagccgctc ccttggcacc ataaggaccg gttgcctcat
     1141 aaagttcaac ggaaacattt tgaatcggag ggatatcttc ggctgtcggc agaatatata
     1201 cggtaaatct ttgattatcc ggaatcccat tccgataatt catattctcg ccgagagtat
     1261 agcctatgga catcgcaatt ccaccttgta tttgcccgtc cattgagagt cggttaatta
     1321 cctttcccgc ttccgtagct gcaaaaatac ccgctaattg cggttgcccg ctgatgggat
     1381 ccaattttaa tttgacagcc tgtgcgataa aggtatacat ggcgtgagga aaaccgatga
     1441 cattcaaatc ggttttcgac tgtggaaatt cagtttcagc gaatcctctc ccagtttccg
     1501 gatgggaaaa ataatctcga acagcagcaa gaattgcatt cccggcgata aagaccgatc
     1561 gggaaccggc tgtggatccg caatcgaaag tctgtgctgt atcagccata atcaggcgga
     1621 ttttttctaa ggggactttc aatgcttcgc cggcaatcgc agccaaagcg gttgcgttgc
     1681 cctgtccgat gtcaaccaat ccgactcgaa cggtaaaatc tccgttcggt tctcgatcaa
     1741 tctccacttt tgccgaatcg ggaacgcctt t
//
LOCUS       DAOMIV010000463         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_78064_length_1770_cov_5.306122, whole genome shotgun sequence.
ACCESSION   DAOMIV010000463 DAOMIV010000000
VERSION     DAOMIV010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78064_length_1770_cov_5.306122"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1301
                     /locus_tag="PKV59_09125"
     CDS             168..1301
                     /locus_tag="PKV59_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46951.1"
                     /translation="MVEIRKVDTADKKQTGQFVQFHYDLYKGDPNWVPPFRSDIYMMM
                     NRKKHPFYEHSDAEFFTAWKDGKMVGRIAGLNNNSYNNFHHLKTVNFFLYDSIDDQEV
                     CNALFDAVAEWGRGKGEDTLVGSKGFSLYDGYGILVEGYEYRQMMNMSSYNYPYYKTL
                     LENYGFEKMNDFVSMNFTLPDFNLPEKVYKAAEIAKKRGYLHVYTFKNKKEIMGSALK
                     IAQAYAKSFENNWEYFPLSEREFISLVDNVIKVVDPKMVKFIYNDKEELVGFLLGFPD
                     ISAAMQRHNGNLNPALILDLLREMKKTKYLSLNGMGILEEYRKKGGNALLYTAILDLL
                     KEHPNIYGMEATQMAESAKEIQVEMLTMGLKPTKRHRVFSKSI"
     gene            1359..>1770
                     /locus_tag="PKV59_09130"
     CDS             1359..>1770
                     /locus_tag="PKV59_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46952.1"
                     /translation="MEIRPLKRDYGISSRFILGILLLVLIAIGGNVYVVLHTDLGAGF
                     YSYWLSSRVLFAQGENPYGSDIFEQIIRRFPEDRYLSGFTLPLYAVIPVFLFSFIDNF
                     EIALIIWMTILEALLVGIGMKTISAFQIDRRTFTP"
BASE COUNT          523 a          327 c          376 g          544 t
ORIGIN      
        1 ctattttctt tcccttaaat ctgtcatcga atcctatttt ctgtaaaatt ttagtcagcc
       61 gtcatattta accttccggt taaggtaaaa tagtgtaaat agccaaaatt atcctctact
      121 attattatca tcattttatt ctttgaggtt aaaggagcct tcatgccatg gtagaaatac
      181 gaaaggtcga tactgcggat aaaaaacaaa caggccaatt tgttcaattc cattatgatc
      241 tatacaaagg agatccgaat tgggttcctc ctttccgatc cgatatttac atgatgatga
      301 atcgtaaaaa gcatcctttc tatgaacatt cggacgctga attttttacc gcatggaaag
      361 acggaaaaat ggtgggacga attgccggat taaataacaa cagctacaac aattttcatc
      421 atttgaaaac cgtcaacttc ttcttatatg attccatcga tgatcaggaa gtttgtaacg
      481 ccctttttga tgccgtggca gaatggggaa ggggaaaagg ggaggacacc ttagtcggtt
      541 cgaaaggatt ttccctgtat gacggttatg gaatattagt tgaaggctat gaatatcggc
      601 agatgatgaa tatgtcatct tataactatc cttactataa aactttattg gaaaattatg
      661 gctttgaaaa aatgaatgat tttgtttcca tgaattttac gcttccggat ttcaacctgc
      721 ctgaaaaagt atataaagcg gctgaaattg ccaaaaagcg cggttatctt catgtctaca
      781 cgttcaaaaa taagaaagaa atcatgggaa gtgctttgaa aatcgcccag gcttatgcca
      841 aatctttcga aaataactgg gaatattttc cgctttcaga gagagaattc atcagtttag
      901 tggataatgt catcaaagtc gtcgatccga aaatggtaaa gtttatttat aacgataaag
      961 aagaactggt tggctttctg ctgggatttc cggatatatc cgccgcgatg caacggcaca
     1021 acggcaatct gaatccggca ctgattctcg atctgttacg agaaatgaag aagacgaaat
     1081 atttgtcgtt gaacgggatg ggaattctgg aagaataccg aaagaagggg gggaatgccc
     1141 tcctttatac ggcaatcttg gatcttttaa aggaacatcc caatatttat ggcatggagg
     1201 cgactcaaat ggctgagagt gccaaagaaa ttcaggtaga gatgcttacc atgggattaa
     1261 aaccgacaaa acggcatcga gttttttcaa aatcgatcta atgaggttcg tcatctgaaa
     1321 aaacttcctg ccggcttttt aaaaaagaga aggaatcaat ggaaatccgt cccttaaaac
     1381 gcgattacgg tatttcatcg agatttattc tcggaatcct tcttttagtt ttgatagcca
     1441 tcggcggaaa tgtttatgtt gtgctccata ccgacttggg agccgggttt tacagttact
     1501 ggctttcgag tcgggttttg tttgctcagg gggagaaccc ctacgggtcc gatatttttg
     1561 aacagattat ccggcgtttt ccggaagacc ggtatcttag cggtttcact ttacctctct
     1621 atgccgtcat cccggttttt ttgttttctt ttatcgataa tttcgaaatc gcattgatta
     1681 tttggatgac gattttggaa gcgttgttag tcggcatcgg aatgaaaaca atctcggctt
     1741 ttcaaatcga tcgccgtacc tttacgccgt
//
LOCUS       DAOMIV010000464         1763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_78533_length_1763_cov_4.364754, whole genome shotgun sequence.
ACCESSION   DAOMIV010000464 DAOMIV010000000
VERSION     DAOMIV010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78533_length_1763_cov_4.364754"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1172
                     /locus_tag="PKV59_09135"
     CDS             102..1172
                     /locus_tag="PKV59_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQP46953.1"
                     /translation="MINLTRGVPPVEVFPIEELIKASEKNLREDGKRVLQYLHAPGYP
                     PLIEYLAKKHGVRKEEVVLGNSSLELLQFVTLTEMKPGDCAYIESPSYDRANTLLKRS
                     GADVVGIPLEIDGVNLDILEKELKKRPPKLFYIIPDFQNPMGTTTCAEKRKQIAKWAE
                     EYDFLVVEDAPYRELRYHGTAVPSIRSFAPDHVVTLSSFSKTLAPGLRTGYLYGREDI
                     VKRITAWEVDTYIGSVTPTQGQVYEYLNQGFFEPNLKKLCDLYRPRLDTLLNSLDKYL
                     PNAVYPRPEGGFFVGVTLPEGNSMEKLLPAAADAGFKLTDGRGFYLNPQDGDRFLRIP
                     FCSLSPEEISNAIQSLAKIIVH"
     gene            1226..>1763
                     /locus_tag="PKV59_09140"
     CDS             1226..>1763
                     /locus_tag="PKV59_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQP46954.1"
                     /translation="MTTSFRNLRPLLNPKSIAIVGASEKHGSAGRIAIENLHYLGYQG
                     EIFAINPKNESVLGHPCYKDLASVGKDIDMVAILIGAPQVKSVLEQMHSLNIPACWCL
                     ASGFSESGPEGKKLQDEIFKYCEENNILLCGPNCIGVANIPDKYAAFSVAIKPNIKAG
                     NISAVMQSGAILMGLANSA"
BASE COUNT          524 a          368 c          397 g          474 t
ORIGIN      
        1 ggttgcatca tagcaaagtc ggacgctttt catttcctcc ggctaaagaa ataaaataaa
       61 taatttttat agatttaaat cttattgaca gaaaggatta tatgatcaat ttaactcgtg
      121 gcgttcctcc ggttgaagta ttcccgattg aagaactgat aaaagcatcc gaaaaaaatc
      181 tgagagaaga cggaaaacgt gttctccaat atctgcatgc gcccggctat ccacccctga
      241 ttgagtattt ggcaaaaaaa cacggagtaa ggaaagaaga ggttgtctta ggaaacagtt
      301 cgttagagtt gctccaattc gtgacactca ccgaaatgaa accgggagat tgcgcctata
      361 tcgaatcacc ttcttatgac cgagccaata cacttctgaa acgctcaggt gccgatgttg
      421 tcggaatacc gcttgaaatc gatggggtta atctggacat tttagaaaag gaactgaaaa
      481 aacgcccccc aaagttattc tatatcattc ccgattttca aaatccgatg gggacgacta
      541 cctgtgcgga aaaaaggaag caaattgcca aatgggcgga agaatacgat tttctcgtcg
      601 tagaggatgc gccttaccgt gagttgcgtt atcatgggac agcagttcct tccatccggt
      661 ctttcgctcc ggatcacgtt gtgacccttt cttcgttttc gaaaacgctc gcaccggggc
      721 ttcggaccgg atatttatat gggcgagaag atatagtaaa acgaatcacg gcatgggagg
      781 ttgacactta tatcggttcg gttactccga cacagggaca ggtttatgaa tatctgaatc
      841 aaggattctt tgaaccaaac ttaaaaaaat tgtgtgattt atatcgcccg cgtttagata
      901 ctttactgaa ttcgctggat aagtatcttc cgaacgccgt gtacccccgt cccgaagggg
      961 gattctttgt cggtgtcaca cttccggaag gaaattcaat ggagaaattg ttaccagcag
     1021 ccgctgatgc cgggtttaaa ttaaccgacg gccgtggttt ttatttgaat ccgcaggatg
     1081 gtgatcgatt cttaagaatt cccttctgca gcctttctcc ggaagaaata agcaatgcca
     1141 tccaaagttt ggcaaagatc atcgtccatt aatttataaa atgaatcaag gggaagaatc
     1201 ctcttcccct tgattaggaa aaattatgac cacttctttt cgcaacctac gccctttatt
     1261 gaatcccaaa agcatagcca ttgtcggagc ttccgaaaaa cacggaagtg ccggacgaat
     1321 tgcaatcgaa aatttacact atttgggtta tcaaggtgaa atttttgcaa ttaatccgaa
     1381 aaatgagtct gtattgggac acccgtgtta taaggatctc gcatccgtcg ggaaagatat
     1441 cgatatggta gcaattctga tcggtgcccc acaagtaaaa tctgttttgg aacagatgca
     1501 ttcgctgaat atcccggcat gctggtgcct ggcaagcgga ttttcggaat ccgggcccga
     1561 aggcaagaaa ctccaagatg aaattttcaa atattgtgag gaaaacaata ttttgctttg
     1621 cggaccaaac tgcatcggcg tcgcgaatat tccggataaa tatgcggctt ttagtgtagc
     1681 gattaaacca aatatcaaag ccggaaatat atcggctgtc atgcaaagcg gagcaatttt
     1741 gatgggattg gcaaattcag cca
//
LOCUS       DAOMIV010000465         1756 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_79011_length_1756_cov_3.806584, whole genome shotgun sequence.
ACCESSION   DAOMIV010000465 DAOMIV010000000
VERSION     DAOMIV010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79011_length_1756_cov_3.806584"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PKV59_09145"
     CDS             complement(<1..882)
                     /locus_tag="PKV59_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQP46955.1"
                     /translation="MDNSIDLKQKFLLDPAIHFLNHGSFGACPKEVIETFFSWTLRME
                     KQPVLFLGREYHELLKEARIILANYLNTSADSVVYIPNATYGVNIMARSINLKPGDEV
                     VTGNQEYGACENVWIDCCQKSGAILKKADIRLPYGSDQELIDCLWEQVTPRTRVIYLS
                     HLTSPTALTFPIREICRKAHEEGIITCIDGAHAPGQLPVDLLSLDCDFYTGNCHKWMC
                     APKGSAFLYVSEQWIQKVAPLIISWGHGPFLENAVGNPLIDFNQWTGTNSPCAALSVP
                     AAIRFMEANHWDQVRRAC"
     gene            1474..1725
                     /locus_tag="PKV59_09150"
     CDS             1474..1725
                     /locus_tag="PKV59_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46956.1"
                     /translation="MREPIEDSEKGILYWQKTLYLSDHPEKYIGEPVEVLGFIYRREE
                     DPKEVFFVGRLLLSCCYDDALPQGLGVYLPDNEKWKNDQ"
BASE COUNT          510 a          364 c          381 g          501 t
ORIGIN      
        1 acaagctctc cgaacttgat cccaatgatt ggcttccatg aatcggatgg cagctgggac
       61 gcttaaggcg gcgcatggac tgtttgttcc cgtccattga ttgaaatcaa tcagggggtt
      121 accgaccgca ttttccagaa aaggaccatg tccccaactg ataatcaagg gtgcaacttt
      181 ttgaatccac tgttcactga cgtacaggaa agccgagcct ttcggcgcac acatccattt
      241 atgacagttc ccggtgtaaa aatcacaatc aagcgaaaga agatccacag gcagctgccc
      301 cggcgcatga gctccgtcga tacaggtgat aatgccttct tcatgtgctt ttctgcatat
      361 ttcacgaatc ggaaaagtca gggcggtcgg agaagtcaaa tggctgagat agattacccg
      421 agtccgagga gttacctgtt cccataggca atctattaat tcttgatcgc tgccatacgg
      481 aagacgaatg tccgcttttt ttaggatagc cccgcttttt tggcagcaat ctatccaaac
      541 attttcgcag gcaccgtatt cctgattgcc tgtgacaacc tcatcgcccg gtttcagatt
      601 gatgctgcgg gccatgatgt tgacgccgta agtggcattg ggaatatata caacagaatc
      661 ggctgaggtg ttcaaatagt tcgctaaaat gattctggct tcttttaata attcgtgata
      721 ttcgcggcct aaaaataaga ccggttgttt ttccatccgc aacgtccatg aaaaaaaggt
      781 ctcgataact tctttcggac aagctccgaa agatccgtga ttcaggaaat gaatagcagg
      841 gtccagcaag aatttttgtt ttaaatcaat tgaattgtcc ataatgctcc ttcgttatca
      901 gtgaataatt atatgttacc gatcattcaa aaacgggaaa gcaagtcggt aaagggtaat
      961 ccggcagcaa acaatcgatc gaatcgcctt ccgaaattcg ttaaccgccg gcaaatcaac
     1021 ttcgagaatt gacttttccg atggctatcc cctttcaagc aaaataactt tgcaatttta
     1081 atgctttttc ctgtggattt tattgtttta taaatttaca aatcgacaac ataactaaga
     1141 aaaaacaacc atatcaaaaa agatcgagcc ggttacaaat ttactcatcc gaagtccgga
     1201 tttcaaaagg cttccatttt tataatttgt cacactgaag atgaactgtt aaatcgatca
     1261 ttaacgataa aatattactg ttgcccgaat aatcggcttc gatcgtatta tttcgaacga
     1321 aagagcggaa attttatgag gttttatcga tttcttatag tctgtatatt gggtttcact
     1381 tttttgataa ccggttgtga atctgctcaa tcgactcttc cgactgaaac tttaatccgg
     1441 gaagagatcg aacctttgtt gcttccggaa agtatgagag aaccaatcga ggattcggaa
     1501 aaagggatcc tttattggca aaaaacgctc tatttatccg atcatccgga aaaatatatc
     1561 ggggaaccgg ttgaagtact gggatttatc taccgccggg aagaggatcc gaaggaagta
     1621 tttttcgtgg gaagattatt gttatcatgc tgttatgatg atgcgctgcc ccagggattg
     1681 ggtgtctatc tgccggataa tgaaaaatgg aagaatgatc aatgagtaac ggtcaaaggg
     1741 cattgggatc gtattc
//
LOCUS       DAOMIV010000466         1751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_79362_length_1751_cov_3.653302, whole genome shotgun sequence.
ACCESSION   DAOMIV010000466 DAOMIV010000000
VERSION     DAOMIV010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79362_length_1751_cov_3.653302"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="PKV59_09155"
     CDS             complement(<1..1225)
                     /locus_tag="PKV59_09155"
                     /inference="COORDINATES: protein motif:HMM:NF014948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HQP46957.1"
                     /translation="MKKITLAVLFGSREFFPGDLVIDARKQMFKALDLAGINYIALDE
                     KVGVHGAVTTYAHAKECAALFKQHMDEIDGILVSLPNFGDEKGIVDTIKLSGLRKPIL
                     VQAFPDDLDKFDVANRRDSFCGKISVCNNLRQYGYNFSLTTDHCVYPYEESFKEDLKK
                     FAGVCRVVNGLKHARLGAIGARPNAFNTVRYSEKLLENAGISVLTADLSEIIGPVYRL
                     SDSDPAVKKALEAIDAYIPHEGVPSEALVKMAKFKAVLDQWIRDNEITGTAIQCWNSV
                     QENLGVNVCTLMSMMSENLIPSACETDITGLLSMYTLQLAGDAPAILVDWNNNYAAKK
                     DECVLFHCGNFAKSYVPNACMGNAKILATTMGTENTMGTIEGRVVGDKMTYARVSTDD
                     VNGMIRAYTGEGKFVD"
     gene            complement(1375..>1751)
                     /locus_tag="PKV59_09160"
     CDS             complement(1375..>1751)
                     /locus_tag="PKV59_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQP46958.1"
                     /translation="SKMETLLQANPDVKGVICGNDTMAMGAYAALVAAGKPDVIVVGF
                     DGSDDVLQSIMDGGIKATGLQPVAAISIAAVDQADYYLTHGEFQKEEKQSIDCVLITI
                     ENACMYKTFAPVEGVTSCEKKQ"
BASE COUNT          471 a          463 c          344 g          473 t
ORIGIN      
        1 cgtccacaaa tttcccttct ccagtataag cacgaatcat accgttaaca tcgtccgttg
       61 aaacacgggc ataggtcatt ttatctccga caacccgtcc ttcgatcgtc cccatcgtat
      121 tttcggttcc cattgttgtc gccagaattt tagcattgcc catgcaggca ttcggcacat
      181 aactcttcgc aaagttcccg caatggaaca agacgcattc atctttctta gcggcataat
      241 tattattcca atccaccaag attgccggtg catctccggc taattgcaaa gtgtacatcg
      301 aaagcagacc ggtgatatcc gtttcgcaag cggaaggaat caagttttcg ctcatcatgc
      361 tcatcagtgt gcagacattg acacccaggt tctcttgaac agaattccag cattgaatgg
      421 ctgtgcccgt tatctcatta tcccgaatcc attgatccaa aacagcctta aattttgcca
      481 ttttgaccaa cgcttcactc gggactccct catgcgggat ataagcgtca atcgcctcaa
      541 gcgctttttt gaccgccgga tcactatcgg aaagccgata gacgggtccg ataatttccg
      601 ataaatcagc cgtcaaaaca gaaataccgg cattctccaa caatttttcg ctgtatcgaa
      661 cggtattaaa tgcattcggg cgagcgccga tcgcccccag tcgggcatgt ttcaaaccgt
      721 tcaccacgcg gcaaactccg gcaaattttt tcagatcctc tttgaaactt tcctcgtacg
      781 gataaacgca atgatcggta gtcagagaaa aattatagcc gtactggcgc aaattattgc
      841 aaaccgaaat ctttccgcag aaagaatccc ggcgatttgc cacatcgaat ttatccaaat
      901 cgtccgggaa agcctgtacc aaaatcggct tccgcaggcc ggacaatttg atggtgtcaa
      961 caatcccctt ttcatcgccg aaattgggca gactgaccaa aattccgtcg atttcatcca
     1021 tatgttgttt aaataaagcg gcgcattctt tggcatgggc ataagttgtc acagcgccgt
     1081 gaacacccac tttttcatcc agcgcaatat agttgattcc cgccaaatcc aacgctttaa
     1141 acatttgttt gcgtgcatcg attacaagat ctccggggaa aaactctcga gatccaaata
     1201 agacagccaa ggtaattttt ttcatttgtt ctcctcacaa ggacatgcga attattctat
     1261 tgaatatatt ctaattttaa ccaaatattt ttcaccagcc ttattttcaa aatgaaagct
     1321 ggtgaaaaat atcagggacg gcttttctca agccgtccct gaattgccaa tcaactactg
     1381 cttcttctcg caggaagtga caccttccac cggagcaaat gtcttgtaca tgcaggcatt
     1441 ctcgatggtg atcaatacac agtcgataga ctgtttttct tccttctgga attcgccgtg
     1501 ggtcagataa taatcagcct ggtcgacagc ggcgatggaa atagcggcaa ccggttgaag
     1561 acccgttgct ttgattcctc cgtccataat ggactgcaga acatcatccg aaccgtcgaa
     1621 accgacaacg atcacatccg gttttccggc agcaaccaat gcggcatatg cgcccatcgc
     1681 catggtatca tttccgcaaa tcacgccttt gacatccgga tttgcctgga gcaatgtttc
     1741 catcttggag a
//
LOCUS       DAOMIV010000467         1748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_79554_length_1748_cov_4.370939, whole genome shotgun sequence.
ACCESSION   DAOMIV010000467 DAOMIV010000000
VERSION     DAOMIV010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79554_length_1748_cov_4.370939"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            246..>1748
                     /locus_tag="PKV59_09165"
     CDS             246..>1748
                     /locus_tag="PKV59_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HQP46959.1"
                     /translation="MKLFRVLKDVFISSLPLAAIIIIVCVFISPLDNQYDYIKLLIGY
                     ISVVFGQVFFLVGIDISILPIGKLVGQSLIKLKKASYIIFFGFLFGLLTTVAEPALSV
                     LARQAHMIFDVVHETTFLWIMGSGIGIFVGFALFRILNDLDIKVVFAILYLFIFIMAI
                     FVPDEFVGLAFDGSGATTGDVSVPFILSLGMGVSATMSRRKTNDDIFGIIGLASVGPI
                     LTLFLYGIFIKMKFGGNIPVSGIYNPGAAEGYKQIIISNLGSVALALFPIIVIFLPFQ
                     FFWIKLPKSELIKMLLGTIPVYIGLLIFLSGIDFGFAFAGKYIGEVFLDPNRPEWFRL
                     ILLAVGFILGVSITLSEPAVTVLGEQLEEITNGHIKKNTIRMTLAIGIGFASVISILK
                     ILLQADILWFLAPLYLIALILMKFTSPLFVGLAFDSGGVSGGALTSAFLTPLTLGVAQ
                     AVANAEGLGAQSVLINGFGIIAFISVTPLITVQILGIIYNLRLKKAQKITA"
BASE COUNT          433 a          289 c          375 g          651 t
ORIGIN      
        1 ataaaaataa ggaaattttt taattttgtt ttaatcgtga tttagacctt gttgttcatt
       61 cggctgattg cttgcttgaa catggaaatt tttatccgat agccatatta tcggataaca
      121 aagtttttga ggtcgcatat ttcgaaagaa taaaaaattg attatgtgga acggaatttg
      181 atgagttcac tttacgactc aacggttgtc gttcaattct atttttgaaa ataaagttgg
      241 gaagaatgaa actttttaga gtattaaaag acgtttttat atcatcatta cctttagctg
      301 cgattattat tattgtctgt gtctttattt ctcctcttga taatcaatat gattatatta
      361 aattacttat cggttacatc tctgttgttt tcggacaggt cttttttctg gttggaatcg
      421 atatcagcat tcttccgatc ggcaaattag tcggtcagtc actgattaag ctgaaaaaag
      481 catcttatat catttttttc ggatttttat tcggtctcct tacgaccgtt gcggaaccgg
      541 cgttatcggt tttagccaga caagcacaca tgatatttga cgtggtacat gaaactactt
      601 ttttatggat catgggatcg ggaatcggta tttttgtcgg ttttgcttta tttcgaattc
      661 tcaatgatct tgatattaaa gttgtttttg cgattctata tctgtttatt tttatcatgg
      721 cgatttttgt cccggacgaa tttgtcggtt tggcatttga cggaagcgga gcgactacgg
      781 gtgatgtttc cgtcccattc attttatctc tcggtatggg tgtgtcagcg actatgtcca
      841 gacgcaagac gaatgatgac attttcggaa ttatcggttt agcatctgta gggccgatcc
      901 tgacgctgtt cttatatggt atttttataa aaatgaaatt tggcggcaat attccggtat
      961 ccggaattta taatccgggg gctgcggaag ggtacaaaca aattatcata tcgaatctcg
     1021 gaagtgtcgc tttagcgctt tttccgatta ttgttatttt tcttccgttt caattttttt
     1081 ggatcaaact tccgaagagt gaattaatca aaatgttact cggaacgatt ccggtttata
     1141 tcggactttt aatttttctt tcgggaattg atttcggttt tgcttttgcg ggaaaatata
     1201 tcggtgaagt attcttggat ccgaatcgtc ccgaatggtt tcgtttgatt ttactggcgg
     1261 tcgggtttat tctcggggta tccataacac tctcggagcc ggctgttact gttttaggcg
     1321 aacaacttga agaaataacc aacggccata ttaaaaagaa tacaattcgg atgacattgg
     1381 cgatcggaat cggttttgca tcggttattt caattttgaa gattctattg caagcggata
     1441 tattatggtt tttagctcct ctttatctta tagctttgat attgatgaaa tttacttcgc
     1501 ctttatttgt gggactggct tttgattccg gcggagtttc gggcggtgca ttgacttcgg
     1561 catttctaac tcctcttact ttgggggtag ctcaagctgt cgcaaatgcg gaaggattgg
     1621 gagctcagtc ggttttgata aacggttttg ggataatcgc ttttatttcg gtgacacctc
     1681 ttatcacggt tcagattctt ggtatcattt ataacctgcg cttgaaaaag gcacagaaaa
     1741 ttacggcg
//
LOCUS       DAOMIV010000468         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_79693_length_1746_cov_3.500887, whole genome shotgun sequence.
ACCESSION   DAOMIV010000468 DAOMIV010000000
VERSION     DAOMIV010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79693_length_1746_cov_3.500887"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKV59_09170"
     CDS             complement(<1..340)
                     /locus_tag="PKV59_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HQP46960.1"
                     /translation="MKIVVIDGQGGSIGKTIIEQLKKNSFSDPIIAIGTNSIATSTML
                     KAGADFAATGENPILVACEDADILIGPMGIIAANSLLGEITPAIAVAVGKSKAEKILI
                     PSNKCKITVAG"
     gene            complement(441..>1746)
                     /locus_tag="PKV59_09175"
     CDS             complement(441..>1746)
                     /locus_tag="PKV59_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46961.1"
                     /translation="TKDDPWERLERFRDEMGGGDKIQSLLRGQNLFAYSPYPDDLLAA
                     FIRQSIESGSKNMRIFDAMNDVRNLWMAILATKAYGGKVEAAMCYTTSPIHTTQYFVD
                     YGKRLLEEGVDRIAIKDMAGLLHPKDAVVLFPALRKEINVPIVLHTHTTTGVALVNLA
                     LGMKYGLDQFDTCISPFAGGPSHSPIEVMAVVAERMGFEHDLDKDAISKAQDVLRVIF
                     EELKGSIPSFGKSRRKPVYFDDVPVDKVDKMVEAIMKTPEDLDTAIAVSREILADLDY
                     PGYDDKVFITQIPGGMISNLQSQMKEMGVTGDMMDAVMEEFPKVRKDVGYVPLVTPTS
                     QIVGSQAAFNVLAGERYAFVSNEFKMILRGEFGKTPGPIDEEFRKKILGDDVEVKKYR
                     PASYLMPVLEDEVNLPYIKSKKDLLLHHVFGNAADEFLKSRK"
BASE COUNT          480 a          447 c          310 g          509 t
ORIGIN      
        1 tcccggcgac ggtaatcttg catttattac tgggaattaa tattttttca gctttacttt
       61 tcccaaccgc aacggcaatc gcgggcgtga tctctcccaa taaggaattc gccgcgataa
      121 tccccatcgg accgatcaaa atatccgcat cttcacacgc gactaagata gggttttcac
      181 cggttgcggc aaaatccgca ccggctttta acattgtcga cgtcgcaata ctattcgttc
      241 cgattgcgat aatcggatcc gaaaatgaat tcttttttaa ttgttcgata attgttttcc
      301 cgatactgcc gccctgcccg tcaatcacca caattttcat cgatcatttc ctttccgtct
      361 attattacgg accatttgaa caaaaagcag gcttgtcttc ttgaatacag gcctgctttt
      421 attcgtcaac ttttataaaa ttactttctt gactttaaga actcgtccgc tgcatttccg
      481 aacacatgat gcaagagtaa gtcctttttg ctcttgatat acggcaaatt gacttcatct
      541 tccaaaaccg gcatcagata agaagcgggg cgatatttct taacttcaac atcatctccc
      601 aaaatcttct ttctgaattc ctcgtcaatc ggacccggcg tttttccgaa ttcgcctctt
      661 aagatcattt tgaactcatt ggaaacaaat gcatacctct ctccggcgag aacattgaat
      721 gcggcttgag atccgacaat ctggctggtg ggcgtaacca aaggaacata accgacatcc
      781 tttcgaactt tcggaaactc ttccataaca gcgtccatca tgtctcccgt aacacccatt
      841 tctttcattt ggctctgcaa atttgaaatc ataccgcccg gtatctgggt aataaaaact
      901 ttatcatcat aacctggata atccaaatcc gccaaaattt ctctgcttac cgcaatagcg
      961 gtatccaaat cttccggagt cttcattatg gcttcaacca ttttatcgac tttatccacc
     1021 ggaacatcat caaagtaaac cggttttctt ctcgatttgc caaaagaagg gatggatcct
     1081 ttcaattctt caaaaataac tcgcaataca tcttgagcct ttgaaatcgc atctttatcc
     1141 aagtcatgtt caaaacccat tcgttcggca acaaccgcca tcacttcaat cggcgaatgg
     1201 gagggaccac ccgcaaacgg ggaaatacag gtatcaaatt gatcaagtcc gtatttcatt
     1261 cccaaagcaa ggttaaccag cgcaacaccg gtggttgtat gtgtatgcag aacaatcggg
     1321 acattaattt ctttccttaa agccgggaac aacacgacgg catccttcgg atggagtaaa
     1381 ccggccatat ctttaatagc aattcgatcg accccttctt ccagaagacg ctttccgtaa
     1441 tcaacgaaat actgagtggt atgaatcggg cttgtcgtat agcacatcgc cgcttcgact
     1501 tttccgccgt atgctttggt cgcaagaatt gccatccaaa gattacgaac atcattcatc
     1561 gcatcaaaaa ttcgcatatt cttactaccg ctttcgatac tttgtcgaat gaatgcggct
     1621 aataaatcat ccggataagg actatacgca aataagtttt ggccgcggag aagagattga
     1681 attttatccc ctccacccat ttcatcacga aatcgttcaa gtcgttccca cgggtcatct
     1741 ttggtg
//
LOCUS       DAOMIV010000469         1743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_79921_length_1743_cov_4.421801, whole genome shotgun sequence.
ACCESSION   DAOMIV010000469 DAOMIV010000000
VERSION     DAOMIV010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79921_length_1743_cov_4.421801"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PKV59_09180"
     CDS             complement(<1..429)
                     /locus_tag="PKV59_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HQP46962.1"
                     /translation="MPNQGIDVQWFISVILLIIGGYLLGSIPSAYLAGKWIRGIDLRE
                     YGSGTVSGSMVWEHVAKWAVVPVGLFDIFKGFLPTWMTLKFGLGESAAVMVGLVAVIG
                     HNWPIYLNFHGGRGLSPFLGNLLALFPLGCFIMLLSLGIGC"
     gene            complement(473..1339)
                     /locus_tag="PKV59_09185"
     CDS             complement(473..1339)
                     /locus_tag="PKV59_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQP46963.1"
                     /translation="MMRKSVVVTDSAAQVPKEAIDKYGIEVIPLLISFDNKNYLDGID
                     ISAQELYQKMRNDKIVPKTSAPTIMQFYQIFEKHIKEGVSEILFVSLSSKLSSTYNAA
                     LDATKMIREKYHDCEIVIYDSLMATIAQGFLAIEAAQRFEEGMSIKNVVTFLDGARKR
                     TGIAAALDTLEYLARGGRIGKAQYVLGTAIQILPILGIDQEGIVAPVANLRGKRKVIP
                     TMVSLIKKQIGAYHKARFAVMEADAHERAEALQKAAMEAFPKNEFPIVEFTPVMGAHT
                     GPGLIGLAYYWE"
     gene            complement(1339..>1743)
                     /locus_tag="PKV59_09190"
     CDS             complement(1339..>1743)
                     /locus_tag="PKV59_09190"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQP46964.1"
                     /translation="LSVIDVQLEKTIVKAPVDGIILARNIEMGETIAPNAVVLTVGQL
                     ENVNLTVYIPETEYGKVKLNEKVSIQVDSFPDETFTGTVVYISDQAEFTPRNVQTIEG
                     RRTTVYAVKIDVPNPDLKLKPGMPADVTFEEK"
BASE COUNT          500 a          450 c          298 g          495 t
ORIGIN      
        1 acaaccaatt cccagtgaaa gaagcataat aaaacaacct aaaggaaaaa gagctaatag
       61 atttcctaaa aaaggactta acccacggcc accgtgaaaa ttgagataga taggccaatt
      121 atgccctatg acagctacta atccgaccat aactgcggca gattcaccca aaccgaattt
      181 caaagtcatc caagttggca gaaagccttt gaaaatatca aaaagcccta ccggtaccac
      241 cgcccattta gcgacatgtt cccaaaccat cgaaccgctg actgttccgc taccgtattc
      301 ccgtaaatca attccacgta tccatttacc ggcaagatat gcagacggaa tcgatcccag
      361 caagtaacca ccgattatca gaaggataac cgaaataaac cactgcacat ctattccctg
      421 atttggcata atatgacctt tccattaatt taatattttt tctcaatccc catcattccc
      481 aataatacgc caaaccgatc agccccggtc cggtgtgagc tcccataacc ggtgtaaatt
      541 ccactatcgg gaattcattc ttcgggaaag cttccattgc ggctttctgc agagcctccg
      601 ccctttcatg cgcatcggct tccatcacag caaagcgagc cttatgatat gcaccgattt
      661 gcttttttat caaagaaacc attgtgggaa taacctttcg tttcccacga agattagcta
      721 ccggggcgac aatcccttct tgatcgattc cgagaatcgg cagaatctga atagctgtcc
      781 ccaaaacata ttgggctttc ccgatccttc cgccgcgagc aagatattcc aacgtatcca
      841 aggcagccgc tatcccggtc ctttttctgg ctccgtccaa aaaagttacc acatttttga
      901 tcgacattcc ttcttcgaaa cgttgcgctg cttcgattgc caaaaaacct tgtgcaatcg
      961 ttgccattag gctgtcataa attacaattt cacagtcatg gtatttctca cgaatcattt
     1021 tggtcgcatc taacgctgca ttgtaagtgc tgcttaattt gcttgaaagt gaaacaaata
     1081 agatctccga aacgccttct tttatatgtt tttcaaagat ttgataaaat tgcataatcg
     1141 tcggcgcgct tgtcttcgga acgatcttat cattccgcat cttctgatat aactcttgtg
     1201 ccgaaatatc aatcccatcc aaataattct tattatcgaa ggaaattaat aacggaatca
     1261 cctcgatacc atatttgtct atagcttctt taggaacttg agccgcacta tctgtaacga
     1321 ccaccgattt tctcatcatc atttctcctc aaaggtaaca tccgcaggca ttcccggttt
     1381 cagtttcaaa tcgggattcg gtacatcaat ttttacagca taaacggtcg ttcttcgtcc
     1441 ttctatcgtc tgcacattcc gcggagtaaa ttctgcctga tcggaaatat atacaacagt
     1501 gccagtgaaa gtctcgtccg ggaacgaatc aacctgaatg gatacctttt cattcagctt
     1561 tacttttccg tattccgttt ccggaatata aactgttaaa ttcacattct ctaattgacc
     1621 gaccgttaat acaaccgcgt tgggagcgat tgtttcaccc atttcaatgt ttcgagcaag
     1681 aattattccg tcaacgggag cctttacgat agtcttctcc agttgaacat caattaccga
     1741 gag
//
LOCUS       DAOMIV010000470         1742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_80025_length_1742_cov_2.362774, whole genome shotgun sequence.
ACCESSION   DAOMIV010000470 DAOMIV010000000
VERSION     DAOMIV010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80025_length_1742_cov_2.362774"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..348)
                     /gene="ssb"
                     /locus_tag="PKV59_09195"
     CDS             complement(10..348)
                     /gene="ssb"
                     /locus_tag="PKV59_09195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQP46965.1"
                     /translation="MTTLQNKVRLIGNVGQQPEIRTFDSGAKLARLSLATVDRRKNFT
                     GEYVSETTWHNLVAWGRHAELIEQYVVKGKEIMIGGTLVNKSYMDKNGDKRYLSEIRI
                     SEIKLLGSNA"
     gene            834..1010
                     /locus_tag="PKV59_09200"
     CDS             834..1010
                     /locus_tag="PKV59_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46966.1"
                     /translation="MDFFHDDEFINTLSTDDRLEIFRYILVGNSDFTRELLEEVLKDY
                     GVGRLAGRWVLGEN"
BASE COUNT          485 a          314 c          395 g          548 t
ORIGIN      
        1 tccgatctat catgcattgc tgcccagcag cttgatttcg gagatccgga tttctgacag
       61 atagcgctta tcgccatttt tatccatata ggacttgttg accagggtgc cgccgatcat
      121 aatttcttta cccttgacga catattgttc gatcaattcg gcatgtcggc cccaggcgac
      181 gaggttgtgc caggtcgtct ccgaaacgta ttcgccggtg aagtttttac ggcgatcgac
      241 tgtagcaaga ctcagcctgg caagtttcgc gccggagtca aatgttctga tttctggttg
      301 ctgacctacg tttccgatca gccggacttt gttttgtagc gttgtcatga tgtaacgatt
      361 taaaaatgtt atcgatgttg ataaaaatct gttcgaaacg gattcgattc gggaagaatc
      421 tcccaaagtc gatcattccg ttcgacaagg caaaggtgaa aacgctgtca atgaatattc
      481 ggtaattaag cgtttgtagt cgtttgtaag cggttacaat tacttacaat cggatattgg
      541 gccgggaaaa tgccgccgga tagctatatt ggaggaaaag tcccggatat attttttgtt
      601 acagcgaaaa ggcatctgtg caagggtgtc attcgattgc tgaagagcag ctgatatagt
      661 tgtattcaac catatcaagg ataaaggatc atgaaatttc tttcctcaaa atattatccg
      721 ccacggacag cttttgtcct ctgcgacgat taccttcacc ggatgattgt ataaccgaat
      781 taataatgga ataaacgatg cgccttaagg agcaaataac atgcgacggc ttcatggact
      841 tctttcatga tgatgaattt atcaacacgc tatcgacgga cgaccgactg gagatcttcc
      901 gctacatttt ggttggcaat agtgatttta cgcgggaatt gttggaagag gtgttgaagg
      961 attatggtgt aggcaggctg gcggggagat gggttttggg ggagaattga acaaggaagt
     1021 aatattttgt cggcatttga tccaaagaat aggttagatt aagcggtcgc ctatgatttt
     1081 gattaggtat atttatggtc aaattaaagc agacatttga ttatttgaga aatatcggtt
     1141 tatctgagat ttaattcttt ttacttcatg aagaagttat tttgatctta tgcaattcct
     1201 taatatggcc agcagaataa aatatcgcag gatcagatta ttgtctcttg gtagaaaggc
     1261 attggctata ttcagcgtat taaagcggca aaataaagtg caagtaactg atttatgaat
     1321 tattttcttg ttatgatttt gataatatca atttcatctt gcgattgaat aatgaatctt
     1381 tggggcatcg attcactttc cgagcctacc gagccactat tcaaatgaag atcgccagat
     1441 atcaatgggg ttgagcgaca atcaaatctt cccaataatt tctttgataa attcaaattt
     1501 cttttatatt tgtttacaaa taaaaaatct tttggatatg gataatgatt ttaaccctca
     1561 aaattcaggt ggaaataatt caggtggtcc cggttttcca ccggaaaatt acttggtttg
     1621 ggccatctta agtacgatct tttgctgtct gccattgggt gtcgtatcga ttatcaaatc
     1681 taccgaagtc aattccaagt ggcaaagagg tgactacgac ggtgccatga ggtcatccgc
     1741 cg
//
LOCUS       DAOMIV010000471         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_80071_length_1741_cov_3.568209, whole genome shotgun sequence.
ACCESSION   DAOMIV010000471 DAOMIV010000000
VERSION     DAOMIV010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80071_length_1741_cov_3.568209"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..>1741)
                     /locus_tag="PKV59_09205"
     CDS             complement(132..>1741)
                     /locus_tag="PKV59_09205"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HQP46967.1"
                     /translation="SVRDFINAAAEELGMQIQFNATVEKAWNYGRNKGSFENLQDRFY
                     ALLYGKNLEPVLLFDERVAFNFITSLAGYIDEPLEQPSITLQGSEVIITPGKSGRSVD
                     RLMTMTLLQILGRNLQTAEIDIPVIQLEPTASNLAEQKQILESLLGQDFVLYAKENDG
                     NQEVDRISADLLAGWINFQPVITGNEVTIEMIPKRDPFYNRLVTVSVKLYKKPQNARF
                     MFNDQTGLIEPIAEAVVGQELDIEATLSNITKAIQSGEHEAEAVLTFTQPKVLSTANG
                     ADLGITELVQSQYTYFFGSSEERVQNIQASAASFYGLLIEPGETFSMAENIGDIDINN
                     GYAEAPVIVGGETVQGIGGGVCQVSTTLFRAAFNYGLPIVERHQHAYRVFYYERTANG
                     NIDPSLAGLDASVYIPVLDLKFTNDTPYWILMETYVNPAASSIQWKFYSTNVNRYVDW
                     TTSGLTNVTKPDPPLYRENPELAPGEIKQVDWEVDGADVEIIRTVYENGAVHLQDRFI
                     TNYEPWQAVFEYGPGTPNMPPEETKEP"
BASE COUNT          448 a          417 c          396 g          480 t
ORIGIN      
        1 gtgccttgaa aaagagactc gtttttcgaa agatgatttt gatattcggc cggatggaaa
       61 agtgagatgg taactgagag ggcttccctc tcaactccct tttacaagag aatcgatggc
      121 agagaaacga gctatggctc cttggtttct tccggcggca tattgggagt tccgggaccg
      181 tattcgaaaa ccgcttgcca tggttcgtaa tttgtaataa agcgatcctg caaatgaaca
      241 gctccgtttt cgtaaacggt gcgaatgatt tcgacatccg cgccgtccac ttcccaatcc
      301 acctgtttga tttcaccggg agccaattcc gggttttctc gataaagggg aggatcgggt
      361 ttggtcacat ttgtcagacc gctggttgtc cagtccacat agcgattgac attggtactg
      421 taaaatttcc attgaatact ggatgcggca gggttgacgt aagtttccat caaaatccaa
      481 taaggcgtgt cattggtgaa tttcaaatcg agcaccggga tatagaccga agcatccaac
      541 ccggcaagtg agggatcgat gttgccatta gcggttcttt cataatagaa aacccgataa
      601 gcatgttgat gtcgttcgac aatcggcaat ccgtaattga aagcggcacg gaacaaagtg
      661 gtactcactt ggcaaacacc gcctccgatt ccttggacag tctctccgcc cacaataacc
      721 ggggcttcgg cataaccgtt atttatatcg atatcgccga tattctccgc catcgaaaaa
      781 gtttcaccgg gttcaatcag taagccataa aaactggccg cacttgcttg gatgttttgc
      841 accctttctt ccgaagagcc gaaaaaatag gtatactgcg actgaaccaa ttcggtgatc
      901 cccaaatccg ccccgtttgc cgtactcaga actttcggtt gagtgaaggt taaaaccgcc
      961 tccgcttcgt gttccccgga ttgaattgct tttgtgatat tgctcaaggt cgcttcgata
     1021 tcaagttcct gaccgacgac cgcttcggca atcggctcaa taagaccggt ttgatcattg
     1081 aacatgaaac gcgcattttg cggcttttta tataatttca cactgaccgt taccaaacgg
     1141 ttataaaagg gatcccgttt gggaatcatt tcaatggtta cttcatttcc cgtgataacc
     1201 ggttgaaaat taatccagcc ggcaagtaag tccgcagaaa ttctgtctac ctcttgattt
     1261 ccatcgtttt ctttggcgta aaggacaaaa tcctgtccca aaagagattc aaggatttgc
     1321 ttttgctctg ccagattgga cgcggtcggt tccagttgaa tcacggggat atcgatctca
     1381 gctgtctgca gatttctccc caaaatctgc aataacgtca tcgtcattaa tcggtcgacg
     1441 gaacgcccgc ttttcccggg agtgatgatg acttccgaac cctgtaatgt gatggaaggc
     1501 tgttccaagg gctcatcgat atatcctgct aaggaagtga tgaagttgaa tgcaacccgt
     1561 tcgtcaaata gcagcaccgg ctccaaattc ttgccataaa gcaaagcgta gaatcgatct
     1621 tgaagatttt cgaagctccc tttattcctt ccgtaattcc aagccttctc gacggtagca
     1681 ttaaattgga tttgcattcc caattcttca gcggcggcgt taatgaaatc gcggacactg
     1741 t
//
LOCUS       DAOMIV010000472         1741 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_80116_length_1741_cov_2.140569, whole genome shotgun sequence.
ACCESSION   DAOMIV010000472 DAOMIV010000000
VERSION     DAOMIV010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80116_length_1741_cov_2.140569"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKV59_09210"
     CDS             complement(<1..334)
                     /locus_tag="PKV59_09210"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQP46968.1"
                     /translation="MKSDDLELMKEFTPEQIEKLDGIISKYKGKPGGLIPVLEEAQIT
                     LEYLPVSVQKRVAQGLNLPLSRVYGVVTFYSFFTMEPRGKHTVRVCLGTACYVRGGKA
                     IAETLEKTL"
     gene            complement(365..>799)
                     /locus_tag="PKV59_09215"
     CDS             complement(365..>799)
                     /locus_tag="PKV59_09215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQP46969.1"
                     /translation="EVKDDGSGMSNQELQKVLDPFYTTKKVRRVGLGLPMLADAAERT
                     GGKLVLTSEKGHGTCLRVVFGLSHIDRQPLGNIEGTMVTLIAGNPQIDFIYRHQRDSK
                     AFILDTKDIKKEIDDIPINHVDILNFLKKNIFEGLKEIGAQA"
     assembly_gap    800..809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(942..1595)
                     /locus_tag="PKV59_09220"
     CDS             complement(942..1595)
                     /locus_tag="PKV59_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HQP46970.1"
                     /translation="MQRFIEEKLDVVAICDHNASENVPYLMRLSEGKSVTILPGMEIT
                     SIEEVHILAIFDNYDKLNGLQHIIYDYLTGKNDESVFGCQVIVNEWDEVEGFNDRLLI
                     GATELSLDAIIEYIHSFGGLAIASHIDRESFSVLSQLGFVDPASPFDALEVSSALGLR
                     KARVKYPELFSYPMIESSDAHFTKDIGSAFTVMYLEEPSTAEIKLAFEKKLGRYVEE"
BASE COUNT          495 a          399 c          315 g          522 t
ORIGIN      
        1 caagtgtttt ctccaatgtt tcggctatcg ctttaccgcc tctgacatag caagccgttc
       61 ccagacaaac gcgaacggtg tgttttccgc gaggctccat tgtaaaaaat gaatagaagg
      121 taacaacacc atagacgcgg ctcaacggta gattcaaccc ttgggcaact cttttctgga
      181 cggatacggg cagatattcc agcgttatct gggcttcttc caaaacaggg atcagtccgc
      241 ctggcttccc cttatacttg ctgatgatgc catccagttt ttcaatttgt tccggtgtaa
      301 attctttcat taattcaaga tcatccgact tcatacttat tgctcctcct tcaacgttat
      361 acagtcaagc ttgcgctccg atctctttta atccttcgaa aatgttcttt tttagaaaat
      421 ttaaaatatc aacatgattt atggggatgt catcaatttc cttttttata tcttttgtat
      481 ctaaaatgaa tgccttgcta tcccgctggt gccgatatat aaaatcaatt tgcggattcc
      541 ccgcgattaa tgtgaccatt gttccctcaa tattgcccag aggctgacgg tcaatgtgac
      601 ttaatccaaa tacaactctt aagcaggttc catggccttt ttccgatgtt aaaaccaatt
      661 ttccgcctgt tctttcggct gcatcagcaa gcataggaag ccccaatccg acacggcgga
      721 ctttttttgt tgtgtaaaag gggtcaagaa ctttttgcaa ttcctggtta gacattcccg
      781 aaccgtcatc tttgacctcn nnnnnnnnna ttaaagtaat tgaagtctcc gaaagaacta
      841 ttgaaagata tcttcaacaa cttcgatcag aacgtttttt gcaccggcac gcgtagaatt
      901 ctctgctatg tctaagatat gcagagacaa ctctaacata tctattcctc aacatatcgg
      961 ccaagctttt tttcgaaagc cagttttatt tccgctgtgg atggctcttc taaatacatg
     1021 actgtaaagg cacttccaat atctttcgta aagtgtgcat cagacgattc tatcattgga
     1081 tagctaaaca attccgggta tttaaccctt gcttttctaa gaccaagtgc agatgaaacc
     1141 tccagcgcgt cgaacggtga cgccggatca acaaatccga gttggcttaa aacactgaaa
     1201 ctttctcgat cgatatggga tgctatggcc agtccgccaa aagaatggat atattcaatg
     1261 attgcatcca aagataattc cgtagcgccg atgagcaatc tatcgttaaa cccctctact
     1321 tcatcccatt cattgacgat gacttggcaa ccgaacacgg actcgtcatt tttaccggtg
     1381 agatagtcat agatgatatg ttgcagacca tttaacttat cataattatc aaagatcgct
     1441 aaaatatgca cttcctcgat acttgtgatt tccataccgg gtaagatagt tacggattta
     1501 ccctctgata atctcatcaa atacgggaca ttttcagaag cattatgatc gcatatggca
     1561 acgacatcga gtttttcctc tataaaccgt tgcataagag ccctcggata catatccaaa
     1621 tctgcacaag gggataaaca ggtatggaca tgaagatcac atctgaacgc cttggacata
     1681 tataaacggc tttctcaaaa aacaaaaaaa agaccaaaga ttggtctttt atggacatta
     1741 t
//
LOCUS       DAOMIV010000473         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_80333_length_1737_cov_3.991082, whole genome shotgun sequence.
ACCESSION   DAOMIV010000473 DAOMIV010000000
VERSION     DAOMIV010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80333_length_1737_cov_3.991082"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1033)
                     /locus_tag="PKV59_09225"
     CDS             complement(173..1033)
                     /locus_tag="PKV59_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQP46971.1"
                     /translation="MCAQRKGGLGRGLGALLPVSDNEISDESVRMVDVSVIQSNPHQP
                     RVDFNEDELKELTDSILEHGVIQPLIVMPEDDGKYTLIAGERRLRAAIAAGLSMVPVI
                     TRSATDQDLLEIALIENVQREDLSPLETAEAYRSLEVNFNLTHDEIAKKVGKNRVSVT
                     NTIRLLKLPEKVRNALSNRMISEGHARALLALNTEVQQLSALQTILLHNLNVRQTEEL
                     VRRMSGEKTGKEDKKKENSPEIKSIEEQLMQSIGTRVSLHPGKKGGTIHIHYYSDEEL
                     EDLVDRFTGR"
     gene            complement(1054..>1737)
                     /locus_tag="PKV59_09230"
     CDS             complement(1054..>1737)
                     /locus_tag="PKV59_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQP46972.1"
                     /translation="ALGKKVLVIDIDPQANATSSLGMDKNEVRYGTYQVLIGSVPITP
                     HIIENRDFHLSILPASPALSGAEIELIDLPNRESMLKNALKPIQEKFDYILIDCPPSL
                     SLLTINALVASKDGVIIPVQCEYLALEGLGQLTNTINQVRKSFPEVNVRGVLLTMYDG
                     RTRLALDVVNEVRKFYPDKVFESIIPRSIRVAEAPSYGQPINYYSKDSSASKAYDALA
                     REILTQDQK"
BASE COUNT          454 a          398 c          340 g          545 t
ORIGIN      
        1 tgccggcaat cggaatatca ttttccagaa gcgaataaca taaaccatcg gaaatgatat
       61 cttccggctc aagccaatca aaatgtttat gaaaataaat tgctctttta tttgattctc
      121 ttaaagcgat cgcttgatgg ggcaagatct gggttatttt caatgtcatg gatcatcttc
      181 cggtaaaacg atccactaaa tcttccagtt cctcatccga ataataatga atatgaattg
      241 ttccgccttt tttcccgggg tggagtgaga cacgcgttcc gatcgactgc atcaactgtt
      301 cctcaatcga cttgatttcg ggtgagtttt ctttcttttt atcttcttta ccggtctttt
      361 ctccgctcat tcttctcact aattcttcag tctgtcggac attcaaatta tgaagaagaa
      421 tggtctgtaa ggcggatagt tgttgtactt cggtattaag ggcaagaaga gcccgtgcat
      481 gcccttcact gatcattcga ttggaaagag catttctgac tttttccggg agttttagca
      541 aacgaatggt attcgtaacg gaaacacggt tttttccgac tttttttgca atctcgtcat
      601 gtgttaaatt gaaattgact tcgaggctac gatatgcttc cgctgtttca agcggagata
      661 ggtcttctcg ctgaacattc tctattaaag cgatttccaa cagatcctgg tcggtagcgg
      721 aacgtgtgat gacgggtacc atacttaatc cggcggcaat cgcggctcgc aatcgtcttt
      781 ctccggcaat taatgtatat tttccgtcat cctccggcat cacaatcaac ggttgtatta
      841 caccgtgttc caaaatagag tcggttagtt cttttaactc atcttcattg aaatcgaccc
      901 gcggctgatg aggatttgac tgaattaccg aaacatcaac cattctgacc gattcatcgg
      961 aaatctcatt atcagagaca ggtaataaag ctcctaagcc gcgccctaaa ccaccttttc
     1021 gttgagcaca cattttcatt ccctccctga tttttatttc tgatcctgcg tcaaaatttc
     1081 tcttgccaat gcgtcatatg ccttactcgc agagctgtct ttcgaataat agttaatcgg
     1141 ctgcccgtaa gacggggctt ccgctactct gatgcttctc ggaataatgc tttcgaaaac
     1201 cttatccgga taaaactttc ttacttcatt taccacatcc agagcgagcc ttgtccgtcc
     1261 atcatacatt gtcaaaagaa cccccctgac attaacctcc ggaaaagatt tacgtacctg
     1321 attgatcgtg ttcgtgagtt gtcctaatcc ttccaaggcc aagtattcgc attgtaccgg
     1381 aataataaca ccgtcttttg atgcgaccag cgcattgatc gtcaataggc tcaatgatgg
     1441 gggacaatcg attaatatat aatcaaattt ctcttgaatc ggttttaaag catttttcag
     1501 catgctttcc cgattgggaa gatcgattaa ctctatttcc gctcccgata atgccggtga
     1561 agcgggaagg atagaaaggt gaaaatctcg attttcgatt atatgagggg ttatgggaac
     1621 agaaccgatc aaaacctggt aagtcccata acgaacttca ttcttatcca tacccaagga
     1681 agatgtagca tttgcttgcg ggtcaatatc gatcaccagt actttttttc ctaatgc
//
LOCUS       DAOMIV010000474         1736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_80391_length_1736_cov_5.026175, whole genome shotgun sequence.
ACCESSION   DAOMIV010000474 DAOMIV010000000
VERSION     DAOMIV010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80391_length_1736_cov_5.026175"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="PKV59_09235"
     CDS             <1..973
                     /locus_tag="PKV59_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQP46973.1"
                     /translation="LAEYGFRLPSALDNRPLTFEEFEKTMGYTIYTSATPGPYELEHS
                     QQIAEQIIRPTGLVDPQVEVRPTKGQIDDLIGEIRKRTAANQRVLVTTLTKRMAEDLS
                     EYLKDNDIKVHYLHSEVDTLERISILRDMRTGVYDVIVGINLLREGLDLPEVSLVAIL
                     DADKEGFLRSGVSLIQTIGRAARNVDGKVILYADKMTDSMKYAIDETNRRRKKQQAYN
                     EAHHITPMTIVKGIYDLSERLTVSNAIADEKADYKTTRKNLPKNELKHILADLEREMK
                     EAAKNLDFERAAELRDQVYELRGLVADVSDASPWQRAKMLAGEIDLK"
     gene            complement(998..1675)
                     /locus_tag="PKV59_09240"
     CDS             complement(998..1675)
                     /locus_tag="PKV59_09240"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HQP46974.1"
                     /translation="MDVKITWLGHSAFLIDFDHYKVLLDPFLTGNSNAAISAKKAEAD
                     LILVSHGHSDHVGDAIEISNRCHAPILCNFELSNWFAKHGAVNTMPIYVSGTRAFPFG
                     TVRATIAVHGSGTPDGTYGGLALGFILATHDQQKKIYFAGDTGVFSEMSLIGEDAVDV
                     ALLPIGDIFVMGLSESIRALKMIRPKNVIPMHYDPGLNLEGWKREVIETSGAKPIVLR
                     SGESFDL"
BASE COUNT          496 a          422 c          392 g          426 t
ORIGIN      
        1 ccttgccgaa tatggcttcc gactgccttc cgctttggat aaccgcccgc tgacttttga
       61 agaatttgaa aaaacgatgg gatacaccat ttacaccagt gccacaccgg gaccctatga
      121 attggaacat tcccaacaaa tcgccgaaca aatcatccgt ccgaccgggt tggttgatcc
      181 gcaggttgaa gttcgaccga caaagggcca aattgatgac ctaattggcg aaattcggaa
      241 gcggaccgct gccaatcaac gtgttttggt aacgacatta accaagcgca tggcggaaga
      301 tttatccgaa tatctgaagg acaatgacat aaaagtgcat tatttacatt ccgaagtgga
      361 taccttggaa cggatttcca tcctgcgaga tatgcgtacc ggcgtctatg atgtgattgt
      421 cggaattaac ttgctgcgcg aagggttgga tttgccggaa gtttcactgg tggcgattct
      481 ggatgcggat aaagaaggct ttttacgatc tggcgtctca ctgattcaaa ccatcggcag
      541 ggcagcccgt aacgtggacg gtaaagtcat tctttacgcg gataaaatga cggattcaat
      601 gaaatatgcc atcgacgaga ccaatcgccg ccgcaaaaaa caacaggcat ataatgaggc
      661 acatcatatt acacccatga caattgtcaa aggaatatat gacctttcag agcgattaac
      721 cgtttccaac gcgatagcgg atgagaaggc ggattataaa accactcgta agaatttgcc
      781 gaagaacgaa ctgaaacaca tcttggcaga cctggaaaga gaaatgaaag aggcagccaa
      841 gaatctggac ttcgaacggg cggcggaatt gcgcgatcag gtttatgaac tgcgcggact
      901 ggttgccgat gtttccgacg cttccccctg gcagcgcgca aagatgcttg ccggcgaaat
      961 cgatctgaaa taatcgtttt acaatcggat ttttcggcta taaatcgaaa ctttctccgc
     1021 ttctcagaac aatcggtttg gctccggacg tttcaataac ttcccgcttc caaccttcga
     1081 ggttcaatcc gggatcgtaa tgcatgggaa tcacattttt aggccggatc attttcaatg
     1141 cccgaatcga ttcagataag cccatcacga aaatatctcc gatcggcagt aaagcgacat
     1201 ccaccgcatc ttcaccgatc aaagacattt cgctgaaaac acccgtatct ccggcgaagt
     1261 aaatcttttt ttgctgatcg tgggttgcta aaataaaccc caatgccaac ccgccatacg
     1321 ttccgtccgg agttcccgaa ccgtgcacag cgatcgtcgc acgcaccgtt ccgaagggga
     1381 atgcacgcgt tccgctgaca tagataggca tcgtattgac cgcgccgtgt ttcgcgaacc
     1441 aattcgataa ttcaaaattg cacaaaatcg gggcatgaca gcgattggaa atttcaatcg
     1501 cgtctccgac atggtcgctg tgcccgtgac tgactaaaat aagatccgct tccgcttttt
     1561 ttgccgatat ggcggcattc gaatttccgg ttaaaaaagg atccaacaga actttataat
     1621 gatcgaaatc gatcagaaag gctgaatgcc ccaaccaggt aatttttaca tccatgacat
     1681 ttcctcactt tctttatcaa ttaattgtaa cgtcagttta aatataaaac attccg
//
LOCUS       DAOMIV010000475         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_80767_length_1731_cov_4.249403, whole genome shotgun sequence.
ACCESSION   DAOMIV010000475 DAOMIV010000000
VERSION     DAOMIV010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80767_length_1731_cov_4.249403"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="PKV59_09245"
     CDS             complement(<1..1082)
                     /locus_tag="PKV59_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQP46975.1"
                     /translation="MEKSKVYFTNLRTITMVNNRQSKLEKLIRTAGIGQIDFDHKYAA
                     IKMHFGEPGNLSYLRPNYAKTVADVVKSLGGKPFLVDCNTLYVGRRKNALDHIDSAYE
                     NGFSPFSTGCHILIGDGLKGTDETLVPINGDYVKEAKIGTAVMDADIFISLTHFKGHE
                     NAGYGGTLKNIGMGCGSRAGKMEMHSSGKPFVDQEKCIGCGKCVKICAWGAPKVTNKK
                     SQIDHDKCVGCGRCIAVCPVDAIEPEWGQSNIILDRKIAEYAYAVVKDRPSFHISLVI
                     DVSPNCDCHDENDLAIIPDVGMFASFDPVALDQACIDAVNQQPPNPGSWLAEAHHTGD
                     HFTDIHPDTNWKECITHAEKIGLGHA"
     gene            complement(1125..>1731)
                     /locus_tag="PKV59_09250"
     CDS             complement(1125..>1731)
                     /locus_tag="PKV59_09250"
                     /inference="COORDINATES: protein motif:HMM:NF019303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQP46976.1"
                     /translation="EPYTERKYTLKGTVCIVDSQLFLEYNEIFTPVQNQIAASNFILI
                     NKTDLVDPARLEKIHETVKKINPHAYLYDTQYGILPIEILKEHLIASDFIGETSNHDW
                     NRPVNYLISSDKTVEQDELIDFLETIKEKILRLKGFLKAPDGYWHIDMVEDFIQLTKL
                     QNHAQLEPELVVIGKDQRDFQSLLKETWERTFKHTPNILPN"
BASE COUNT          467 a          398 c          322 g          544 t
ORIGIN      
        1 tccgcgtgac ccaagccgat tttctcagca tgtgtgatac attctttcca attcgtatcg
       61 ggatgaatgt cggtgaaatg atccccggta tggtgggctt ccgccaacca actgcccggg
      121 ttaggcggct gttgattcac ggcatcgata catgcctgat caagtgccac tggatcgaat
      181 gaagcgaaca ttccgacatc cggaataatt gccaggtcgt tttcatcatg acaatcacaa
      241 ttcggtgaga catctattac aagactgata tgaaaggaag gccgatcttt aacaaccgcg
      301 taagcatatt cggctatttt acgatccaga ataatattcg attgtcccca ttccggttcg
      361 atggcatcca cggggcatac cgcaatacag cgcccgcatc cgacacattt atcatgatcg
      421 atttgcgatt ttttattggt gactttcggc gctccccacg cacaaatttt gacacatttt
      481 ccgcagccga tacatttttc ctgatcaaca aacggtttac cgctgctatg catttccatt
      541 tttccggctc tggaaccaca tcccataccg atatttttta atgtaccgcc ataaccggca
      601 ttttcatgcc ctttaaaatg agtcaaggaa ataaaaatat cggcatccat tacagctgta
      661 ccgattttcg cttccttcac ataatcgccg ttaatgggaa ccaatgtttc atctgttcct
      721 tttaatccgt ctccgatcaa aatatggcaa ccggtgctga acggtgaaaa accgttttca
      781 taggctgaat caatatggtc gagagcattt tttcgtcgcc cgacataaag tgtattgcag
      841 tcgactaaaa atggctttcc tcccaggctc ttcacgacat ccgctacggt ttttgcataa
      901 tttggtctta aataagaaag atttcccggt tcgccgaaat gcattttaat tgccgcatat
      961 ttatggtcga aatcaatttg tccgattccg gcggtgcgga tcaatttttc caatttgctc
     1021 tgacgattgt tgaccatcgt aatcgtccgt aaatttgtaa agtacacttt tgatttttcc
     1081 atagatttat atcctcaatt gtctttctaa gttcttccgg atgattaatt cggaaggata
     1141 ttcggagtat gtttaaaagt tcgctcccaa gtttctttaa gaagcgattg aaaatcccgc
     1201 tgatcttttc cgataacaac cagttccggc tctaattgag cgtgattctg caattttgtc
     1261 aattgaataa aatcctctac catatcgata tgccaataac cgtccggagc ttttaagaac
     1321 cccttcaaac gaaggatttt ttccttaata gtctcaagga aatcaatcaa ttcatcttgt
     1381 tctactgttt tatccgaaga gatcaaataa ttcacaggac gattccaatc atgattgctg
     1441 gtctccccta tgaagtcgct cgctattaaa tgctccttta gaatctctat cgggagaatt
     1501 ccatattgtg tatcatacaa atacgcatga gggttaattt tctttaccgt ttcatgaatc
     1561 ttttccaaac gggctggatc aacaaggtcc gttttattaa tcagaataaa attgctggct
     1621 gcaatctgat tttgaaccgg agtaaaaatc tcgttgtatt ccaaaaacaa ttgactgtca
     1681 acgatacaaa ccgtaccctt taaggtatat ttgcgttcgg tatatggctc a
//
LOCUS       DAOMIV010000476         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_80782_length_1731_cov_3.309069, whole genome shotgun sequence.
ACCESSION   DAOMIV010000476 DAOMIV010000000
VERSION     DAOMIV010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80782_length_1731_cov_3.309069"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PKV59_09255"
     CDS             complement(<1..652)
                     /locus_tag="PKV59_09255"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46977.1"
                     /translation="MASRFKGSIRNRFIFLADLILIVVCVFCSYILRLELGYTFYKYL
                     PSAYWMIGVSLVIKPTVYYFFGLYRRLWNYASMNEMKLILIAVTCASAVVSAVMVILA
                     TANVFFGFPRLVLIIDWILSMIAVGGVRFSVRIMYDLDRKNSIKSREKMKRALIIGAG
                     DAGALVLKEIQKNPQLNVHPVGFIDDDPKKMNQSIYGVKVIGTVNDIRKIIDRYAIT"
     gene            complement(656..>1731)
                     /locus_tag="PKV59_09260"
     CDS             complement(656..>1731)
                     /locus_tag="PKV59_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HQP46978.1"
                     /translation="PRRGVASFLISCAVLSILWRLFYIRKFTLPKYLINTLIVGAGKA
                     GCRIAEIIRGIDPSPFSVAGFIDDDPEKIGKEFFGYPVLGDSNSFYKIVEEYQIGQVI
                     MAISNRMNPELFDALTMAEEKGLIVKTMPNVYEELLKRIPVYLLGTDWMVRDFYDQAH
                     ASIFFEITKRLIDIFGGFIGTVFFAILFPFISIAILIDSGRPIFYSQERLGLWGTPFN
                     ILKFRTMVQDAEADGIPRPASYEDNRITRVGHFLRKSHLDEMPQFLNVLRGDLSLVGP
                     RAERKEIIEELQKEIPFYRGRLLVRPGITGWAQINYGYAAGAEQNAIKLEYDLYYIKN
                     RNLLLDFSILLQTFKTIFGLKGR"
BASE COUNT          500 a          408 c          388 g          435 t
ORIGIN      
        1 cagtgatggc gtaccggtcg atgattttcc gaatgtcgtt gacggttccg atgactttga
       61 caccatagat ggattgattc atttttttgg gatcatcgtc gataaagccg accgggtgaa
      121 cgtttaactg cgggttcttt tgtatttcct tcaggaccaa tgcgcccgca tctcccgctc
      181 cgataatcag tgcgcgcttc attttttctc tggatttaat cgaattcttc cggtccaaat
      241 catacatgat tctgaccgaa aaacggacgc cgccgactgc gatcatggag aggatccagt
      301 cgatgatcag aaccaatcgc gggaatccga agaagacgtt ggctgtcgcc aggatcacca
      361 tgaccgccga aacaaccgcc gatgcacagg tgaccgcaat cagaatcagt ttcatctcat
      421 tcatgctggc gtaattccaa agcctgcgat acagcccgaa gaagtaatac accgtcggct
      481 tgataaccag agaaacgccg atcatccaat aagcggaagg caagtattta taaaaggtgt
      541 aacctaactc taagcgcagg atgtaactgc agaatacgca tacaacaatt aaaatcagat
      601 ctgccagaaa aataaaacgg ttgcgaatgc tgcctttaaa gcgggatgcc aaagattatc
      661 ttcctttcaa accgaaaatc gtcttaaaag tctgcagaag gatggaaaag tccagtaaca
      721 ggttgcgatt tttaatataa tacaaatcat attccagttt aatcgcattt tgttcggctc
      781 cggcggcata cccgtaatta atctgcgccc aaccggtgat gccgggtcga accagcagtc
      841 tgccccgata aaaaggaatt tctttttgta attcttcgat aatttccttt cgttcggcac
      901 gcggtcctac caggctgagg tcacctctca aaacattcag gaattgcggc atttcatcca
      961 gatgactttt ccggaggaaa tgacccaccc ttgtaatgcg attatcctca taagaagccg
     1021 gccgcggaat gccgtctgct tcggcatcct gaaccatagt acgaaatttc agaatattga
     1081 acggtgtgcc ccacagcccg agcctttcct gagaataaaa aatgggtctg ccggaatcga
     1141 tcaaaatggc aatggagata aacggaaaca gaattgcaaa aaaaaccgtg ccgatgaatc
     1201 cgccaaagat atcgattagg cgcttggtaa tttcgaaaaa aatgctggcg tgtgcctgat
     1261 cataaaaatc acgtaccatc cagtcggttc cgagcagata aaccggtatc cttttgagca
     1321 gttcttcata aacattgggc atagttttga cgatcaaccc tttttcttcc gccatcgtca
     1381 aggcatcaaa taattcggga ttcattcggt tgctgatcgc catgattacc tggccgatct
     1441 gatattcttc cacgatttta taaaaggaat ttgagtcccc caaaacgggg tatccaaaga
     1501 attcctttcc aattttttcg gggtcatcgt cgataaaacc ggcgaccgaa aatggagatg
     1561 gatcgatccc tctgatgatt tctgcgatgc ggcagccggc ttttccggca ccgacaatca
     1621 aagtattgat cagatatttg ggtaaggtaa atttccgaat ataaaaaagt cgccaaagga
     1681 tggaaagaac ggcacaactg atgaggaaag atgcgactcc gcggcggggc a
//
LOCUS       DAOMIV010000477         1731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_80788_length_1731_cov_3.141408, whole genome shotgun sequence.
ACCESSION   DAOMIV010000477 DAOMIV010000000
VERSION     DAOMIV010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80788_length_1731_cov_3.141408"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1191
                     /locus_tag="PKV59_09265"
     CDS             208..1191
                     /locus_tag="PKV59_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQP46979.1"
                     /translation="MIQRNQRIKLIALTGILILCLFLSIFAGRYPELGFLSPATLKED
                     TTARNIFYNLRIPRIILGLLLGMALGSSGLFFQTVFNNPLVDSGMLGVTQGASFGAAI
                     GIVFFHSNPWIVQLLAIGLGMSGLFLSVVSARKIHYGGWILRLILSGSVISALFGAGL
                     SLLKVLADSRNQLHAIEFWLMGSLGFTIWQGLPLVIVITCVCLFILWKYRWRMNLLAL
                     DSEVASSLGVRIRMEQMLLMTAAVFAVSAATSLSGIIGWVGLIVPHLSRRLFGSDTQY
                     SLPGSMLIGAILILACDNGARILSPSEIPLGIITSFVGVIIFILLMGGKKK"
     gene            1188..>1731
                     /locus_tag="PKV59_09270"
     CDS             1188..>1731
                     /locus_tag="PKV59_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQP46980.1"
                     /translation="MSPKETVLELRDIQFSYDKSDSFSLSSINIKMHSGQIVVILGPN
                     GSGKTTILKIANGRLQPDSGGVYLEGKPLQSFSRREIGQRIGFVPQAEKISFEYTNLE
                     YVTMGRTPWLNPLERPSEKDIEISREAIREVGMGKFMDLPVTKLSGGQSQLILLARAL
                     AQETEILLLDEATSQLDLANK"
BASE COUNT          421 a          346 c          418 g          546 t
ORIGIN      
        1 cattccggca ataggcggtt tttccggcgc cgcgcgatcc ggttattata agaatttctt
       61 ttttatctgc cataagtttt ataaatccac tcgactcgct ctatcagaga attctattct
      121 tccgtctgaa ttttgattat acgtcttttc gaaaatgtgg cataatttga atattcataa
      181 attgattatt taagaatcgg ttatggcatg atacaacgaa accaacggat aaaattgatc
      241 gctttgacgg gaattttgat tttatgtttg tttctctcca tatttgccgg ccgttatccc
      301 gagcttgggt tcctttcgcc agcgacttta aaagaagaca ctaccgcacg gaacattttt
      361 tataatttgc gtattccgcg tattatttta ggtctgttat tgggaatggc attgggatca
      421 tccggtttat tttttcaaac ggtgtttaat aatcctttgg ttgattccgg aatgttaggc
      481 gtgacacagg gtgcctcatt cggtgcggca atcggaattg ttttttttca ctcaaatcct
      541 tggatcgttc aattgttggc gatcggtttg ggaatgtccg gcttgttcct ttcggtcgta
      601 tcagcccgga aaattcatta tggtggttgg atcttacgct tgattttatc cggaagcgtt
      661 atttctgcgc ttttcggagc tggattatct ctgttgaagg ttctcgccga ttcgcgaaat
      721 caactgcatg caatcgaatt ttggctgatg ggaagtttgg gcttcacgat atggcagggg
      781 cttcccttgg tgattgttat tacgtgtgtc tgtcttttca ttctgtggaa ataccggtgg
      841 cggatgaatc ttttggcttt agattcggaa gtggcatcct ctttgggagt tcgaataagg
      901 atggagcaga tgctcctgat gaccgccgcc gtattcgcag tttcggcggc aacctcgtta
      961 agcggaatta tcggatgggt tgggttgatc gtcccccatc tttcccgaag gttattcggt
     1021 tctgacactc aatattctct tcccggaagt atgttaatcg gagctattct gatcttagcc
     1081 tgtgataacg gagcacgaat attaagcccc tcggagatcc ctttggggat cataacttct
     1141 tttgtcggcg taattatttt tattttgttg atgggaggaa aaaagaaatg agcccgaaag
     1201 agacggtact tgagttacgg gatattcaat tttcgtatga taaatccgat tcattttcac
     1261 tttcctcgat caatatcaaa atgcattcgg gacaaatcgt cgttatttta ggtcctaacg
     1321 gttccgggaa gacgaccatc ctgaaaattg cgaacggaag attgcagccg gacagcggcg
     1381 gggtctacct ggaaggaaag ccgttacaaa gcttcagcag acgggaaatc ggccaacgaa
     1441 tcggttttgt cccgcaagcc gaaaaaattt cctttgaata taccaatttg gaatatgtca
     1501 caatggggag aaccccttgg ctgaatcctt tagagagacc ttccgaaaag gatattgaga
     1561 tttcgcggga ggcgatccgg gaagtgggga tgggaaaatt tatggatttg ccggtcacaa
     1621 aactcagcgg cggacagtcc caattaatct tattagccag agcactcgca caagagactg
     1681 agatcctctt attggatgaa gcgacttcac aattggacct ggcgaataag c
//
LOCUS       DAOMIV010000478         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_82408_length_1709_cov_2.895405, whole genome shotgun sequence.
ACCESSION   DAOMIV010000478 DAOMIV010000000
VERSION     DAOMIV010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82408_length_1709_cov_2.895405"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..931
                     /locus_tag="PKV59_09275"
     CDS             137..931
                     /locus_tag="PKV59_09275"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HQP46981.1"
                     /translation="MENKAQIVYFSHGGGPLPILGEPNHRAMIDFMKNLPSRLNRPEA
                     ILVISAHWEEQHPTVLGASHPSMFYDYYGFPEKAYQIQYPAPGDPELAKRIVGMLEKN
                     HIQARIDLNRGFDHGLFIPLELMYPEANIPSIQLSLIRGLDSAAHVSLGKALRDLVSE
                     NILILGSGFSFHNQLAFFDSQSEYADPENDAFQNWLIETCTGNFSQEEREQRLIEWEE
                     APYARYCHPREEHLLPLMVCQGLANKPAKLIFDDAILRKRCIAFEW"
     gene            1029..>1709
                     /locus_tag="PKV59_09280"
     CDS             1029..>1709
                     /locus_tag="PKV59_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQP46982.1"
                     /translation="MTRVMFNRMVTDEQKSRLTKDFPEVDFVFTDSVADPKLLASEGA
                     KSDVLVGVESPDLLNAILSVPNQVRWLHSWFAGVNHLPLDLLEEHHVWLTRAGGVAVN
                     PIVETTICLMIMLTRNFRKYGKQQDAHQWIQLEWPDEVHGKTIGILGMGIIGKGIAKM
                     AKAFDMEVWGLQQFPQPNEYTDHIVGNDELDKLLNAADYVVNILPLTAATKYLMNEER
                     FNQMKKGAY"
BASE COUNT          492 a          318 c          415 g          484 t
ORIGIN      
        1 tcataaataa ccgttatgcg atcgtcggag ctcaacccta tgatagtttt cttcaagtta
       61 ttcggaaaat cgatttgcct cataatcaag agtcaattca atgaaatata ggactttcga
      121 tagggaacga aaataaatgg aaaataaagc tcaaatcgtc tacttctccc atggtggagg
      181 acctctgcca attttaggag aaccgaatca tcgggcgatg atcgatttta tgaaaaactt
      241 accgtcccgg ttgaacaggc cggaggcgat attggtgatc agcgctcatt gggaagagca
      301 gcaccccact gttttaggag catcgcatcc gtccatgttc tatgattatt acggttttcc
      361 ggaaaaagca tatcagatcc aatatcctgc gccgggggat ccggaactcg caaaacggat
      421 tgtcggaatg ctggaaaaaa atcatattca agcaagaatt gatttgaatc gcggatttga
      481 tcacggattg tttatcccgt tggaattaat gtatccggag gcaaatattc cctcgattca
      541 actttctttg attcgtggat tggattcggc tgctcatgtt tctttgggaa aagcgctccg
      601 tgatttggtt tccgagaata tcttgatcct cggttccgga ttttcttttc acaatcaact
      661 tgcttttttt gattctcaat cggaatatgc ggatccggaa aatgatgctt tccaaaattg
      721 gctgatcgaa acctgcaccg gtaacttctc tcaggaagaa cgcgaacaaa gattaatcga
      781 atgggaagaa gcgccttatg ccaggtattg tcatccgcgc gaagaacatt tacttccttt
      841 gatggtttgt caggggctgg caaacaaacc cgcaaaatta atcttcgatg atgcgattct
      901 gagaaaacga tgcattgcct ttgagtggta atttgacaaa tcatatataa taaaattaaa
      961 gaatagcgga gccgaatcgt aaagtattcg aaataataag gtcttatgaa ggaagagagg
     1021 agggaaaaat gacacgtgtc atgtttaata ggatggttac cgatgaacag aaaagtcgtt
     1081 taacaaaaga ttttccggaa gttgattttg tctttaccga ttctgtcgcc gatccgaagt
     1141 tattggcttc ggaaggtgcc aaaagtgatg ttttggtcgg agtggagtcc cccgatttat
     1201 tgaatgctat tttatcggtt cccaatcaag tccggtggct gcattcttgg tttgcgggag
     1261 tcaatcattt gccgctggat cttttggaag agcatcatgt ttggttaacc cgagcgggtg
     1321 gagtggcggt gaatccgatt gtggaaacaa ccatttgcct gatgattatg ctgactcgaa
     1381 attttcgtaa gtatgggaag caacaggatg ctcaccaatg gattcaattg gaatggccgg
     1441 atgaggttca cggaaagaca atcggaattc tgggcatggg aatcatcggt aaaggtatcg
     1501 cgaaaatggc aaaggctttc gatatggaag tttggggttt acaacagttt ccgcagccga
     1561 atgaatatac cgatcatatt gtcgggaatg acgaattgga caagctgctg aatgcggcgg
     1621 attatgttgt taatattttg cccctcacgg cagcgacgaa atatctgatg aatgaagaac
     1681 gctttaatca aatgaagaaa ggtgcctat
//
LOCUS       DAOMIV010000479         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_82886_length_1703_cov_2.792476, whole genome shotgun sequence.
ACCESSION   DAOMIV010000479 DAOMIV010000000
VERSION     DAOMIV010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82886_length_1703_cov_2.792476"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1302
                     /locus_tag="PKV59_09285"
     CDS             211..1302
                     /locus_tag="PKV59_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQP46983.1"
                     /translation="MSKKLFSVMLAIAVVFSIFGIVAAEDAPIKVVLLINGSLGDKGF
                     YDSAASGVRRMEKELGAQTKIIEMGRDETAYEGHYLDVSEQDWDLIISGTWSVKELAQ
                     NIAVQFPEKHYIFFDGNVDRTVVTTGNMMGISYLDNEGSFMAGALAALMLDSGAEKID
                     PDKRILGFVGSMDTANINNFLVGYIEGIKYIDPSIKLLTSYVGSFEDVPKALEMTTQL
                     YNQGAQIVFAPASQSILGAVTASSNVDKYFIGCDMDTWAQLQDTDANLARNIISSALK
                     NVGDSLVTAVKGYMDGTMDTSHDYSLGLSTGAVGLAKNDNYKALVPEEIQKKLDEIEK
                     KIIDGEIVVGSAFGMKSEDVAALRDAMKP"
     gene            1470..>1703
                     /locus_tag="PKV59_09290"
     CDS             1470..>1703
                     /locus_tag="PKV59_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQP46984.1"
                     /translation="MEKTAEALRLQNITKVYPNGVIANNGVTFSVNYGEIHALSGENG
                     AGKTTLMKILFGEEEATDGEIYIDGEKVRITSPL"
BASE COUNT          519 a          296 c          412 g          476 t
ORIGIN      
        1 atgaaaatga ctgttcccaa aaatgatgtc gttcttttct tagaagagat tatgcgggat
       61 gaaaaagacc aacctatcgg atacgcaaga tattatctta ggagcagttg ttatcgaatt
      121 ccgatcaata gacgagccgt aatgtcatat taattattta tttgtcagaa gcggggaaaa
      181 cctgacccgt ttgaagaaaa aggaggaatt atgagtaaaa aattgttttc agtaatgttg
      241 gcaattgccg tggtattttc catattcgga attgtcgcgg cagaagacgc accgatcaag
      301 gtcgttttgc ttattaatgg cagtttgggt gataagggct tttatgattc ggcagccagc
      361 ggtgtccggc gaatggaaaa agaactcggc gcccaaacga aaatcatcga aatgggacgc
      421 gatgaaactg cttatgaggg ccattatttg gatgtgtctg aacaagattg ggatctgatt
      481 atttccggta catggtctgt gaaagagctt gcacagaata tcgctgttca attccccgaa
      541 aaacattata ttttctttga cggtaatgtg gatcggacag ttgtcacgac gggaaatatg
      601 atgggaattt cctatcttga taatgaagga tctttcatgg cgggtgcact ggcagctttg
      661 atgcttgatt caggtgccga aaaaatcgat cccgataaac gaatactcgg tttcgtcggt
      721 tcgatggaca ccgcaaatat taataacttc ctggttgggt atatcgaagg aatcaaatat
      781 attgatccga gcattaaatt gcttacttcc tacgtcggat cttttgagga tgtcccgaaa
      841 gccctcgaaa tgaccaccca actttataac cagggagcac agatcgtatt tgcgccggct
      901 tctcaaagca tcctgggtgc tgtaactgct tcaagcaatg ttgataaata ttttatcggt
      961 tgcgatatgg atacttgggc acaacttcag gatacagatg cgaatctggc cagaaacatt
     1021 atttcttccg ctttgaaaaa tgtgggagat tcgctcgtta cagcggttaa aggttatatg
     1081 gacgggacga tggatacgag tcatgattat tctctcggtc tgagcacagg tgctgtcggt
     1141 ttagcaaaga acgataatta caaagcactg gtaccggaag aaattcagaa aaaactggat
     1201 gaaattgaga agaaaattat tgatggcgaa atcgtcgtcg gttcagcctt cggaatgaaa
     1261 agtgaagatg tcgcggctct tcgtgatgcg atgaaaccgt aacaaccatt cgaaaaatga
     1321 gagtatggat ttatttccat actctcatgt tttatttcat ttaaattttg aattaatgag
     1381 gaaagatgaa tatgaagtat cgaatgccgg agagattact gtttgttgtt ctccaacgtc
     1441 atatttcaaa ggttaaaaag aggtagtcga tggaaaaaac agctgaggca ttaaggctgc
     1501 aaaatatcac caaagtatat ccgaatggcg tcattgcaaa taatggtgtg acatttagtg
     1561 ttaactatgg tgaaatccat gcgctttcgg gtgaaaacgg cgctgggaaa acgacgctga
     1621 tgaaaattct tttcggcgaa gaagaagcta ccgatggtga gatctatatt gacggagaaa
     1681 aagtccggat aacatccccg ctg
//
LOCUS       DAOMIV010000480         1702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_82949_length_1702_cov_3.374621, whole genome shotgun sequence.
ACCESSION   DAOMIV010000480 DAOMIV010000000
VERSION     DAOMIV010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82949_length_1702_cov_3.374621"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1001)
                     /locus_tag="PKV59_09295"
     CDS             complement(153..1001)
                     /locus_tag="PKV59_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HQP46985.1"
                     /translation="MIGIITDSTCDIPESLIKQYGIVVLPQMIIWDNQQYRDRIDMEP
                     EEFYQRLISDPERPTSSQVGIPDFKQAYEKLAEQGASEIIVLTVSGAMSGTYQNAVQA
                     AKLVKIPVHVVDSKGPTMSLGWQVLAAARAREEGMDIKGILNRINQVRERLVQYISLE
                     SLEYLQKGGRIGGAIKWVGSKLQVKPLVSVNLQSGLVEAVGLARTHRHVVEMLYKKFF
                     DGLKSYSNLHIAVLHGNVATEAEALAKKIQDEFHPVELIVNITGPVLGLNTGPGALAL
                     CGYAEE"
BASE COUNT          492 a          378 c          326 g          506 t
ORIGIN      
        1 acgacccgcc atgtagatat tcttaccaac gcaaaagttt attttatcac atcattaatt
       61 ctgctgcaat ttttatctga aatttcatga tgtgacagat ccgttttctt tttgcctgat
      121 aacgaaaaac agatattaat tattcgttga tattactctt ccgcatagcc gcacaaagcc
      181 aatgcgccgg gaccggtgtt caatcctaaa accggaccgg taatatttac aatcaattca
      241 acaggatgaa actcatcctg aatttttttt gccagagctt ccgcttccgt ggctacgttg
      301 ccgtgtaaaa ccgcaatgtg gaggttgcta taacttttta atccgtcaaa gaatttctta
      361 tacagcattt caactacgtg tcggtgggtg cgtgccaatc cgaccgcctc cacaagaccg
      421 gattgaaggt tgactgaaac caatggtttt acctgcagct tgctgccgac ccatttgatt
      481 gctccgccga tccgcccgcc tttttggaga tactcaagcg actccaggct gatatactga
      541 accaatcgtt ctcttacctg attaatccgg ttgagaattc ctttgatatc cattccttcc
      601 tcacgagcac gagccgctgc cagcacttgc cagcccaaag acatcgtcgg acctttggaa
      661 tctacgacat gaacgggaat ttttaccaac tttgctgctt gcacggcatt ttgataagta
      721 ccgctcatgg ctccgctgac ggtaagcaca atgatttctg atgctccctg ttccgcgagt
      781 ttttcatacg cttgtttaaa atcgggaatc cccacttgag aagaagtggg acgttccgga
      841 tccgagatca accgttggta aaattcctca ggctccatat ctattcgatc acgatattgt
      901 tgattgtccc aaatgatcat ctgaggaaga acgacgattc catattgttt tatcagcgat
      961 tcgggaatat cgcatgtact atccgtaata attccgatca attttttact cctgaaacaa
     1021 aatattttta ttccttattg atttatttta cttttattcc aatcataaaa cgataaatca
     1081 gtatgtggat ctgcatttca aatttccatt gtcaagggaa aatgacaaaa aaggcaggtc
     1141 ctaaaaattc gtttttacaa ttcaaaacaa agaaaggaac ttccaatgac aaaagaggcc
     1201 aagataagga taaccatcga aatcggaaat agccaaaaga tatcagctga attcgtgatt
     1261 caatcgggat tttttttggg caacgaatgt cgggaaatcc gttgcaattc ggatgaaaac
     1321 ttgaggctgg cggtcgagat cccttgcgga tactatcctt tcccatatca cggaattggc
     1381 tgatcatccc ttcaggcaca aagagatcaa agcaaaagat caggtaaaat ctcgtgaatc
     1441 aagaaggaag aaatcgaaag tcgctgagtc ttgcgggcag caggcttctt ttctcattct
     1501 gaacgccgga aaagcttccg ttccatattt cgatctttac aggaatataa gttactcaga
     1561 aactatttcc taaattattt ccatgggaat ttgaaacaga tactggtgaa agccacaaat
     1621 caagagtcaa atccctttgt tgttatcggt tttcatcagg aaatcgattg tttttggaaa
     1681 tgctcatttt aatcagcatt tc
//
LOCUS       DAOMIV010000481         1700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_83083_length_1700_cov_3.462614, whole genome shotgun sequence.
ACCESSION   DAOMIV010000481 DAOMIV010000000
VERSION     DAOMIV010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83083_length_1700_cov_3.462614"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKV59_09300"
     CDS             <1..202
                     /locus_tag="PKV59_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46986.1"
                     /translation="PLIGYAAGAALVKEAVEKKVPIRRLAHEKAEAGLLKRSDNGQPI
                     SPAEIDHAFSDLLRMTQGGFVR"
     gene            complement(227..589)
                     /locus_tag="PKV59_09305"
     CDS             complement(227..589)
                     /locus_tag="PKV59_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQP46987.1"
                     /translation="MPLYDYFCKNCGKKFEIRISYEDYGKMDVICPFCRSTFVERAIQ
                     PVSVVQSDQFRMVQEADSDSFNEPYDDPRELGKMMRSFKDQSGEPMAPEFDEVAERLE
                     RGDSIDSIDQDFADMDID"
     gene            822..1499
                     /gene="gmk"
                     /locus_tag="PKV59_09310"
     CDS             822..1499
                     /gene="gmk"
                     /locus_tag="PKV59_09310"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQP46988.1"
                     /translation="MEWFWDELAKNHQDIADMELSLPSFKILKSDPMIVIISGLSGSG
                     KDSVINALRNNSKLNFHFVVTCNTRKQRDDEIDGVHYHFISREKFLDMIRKNEMIEYS
                     PVYNDYKGVPKFEIEKGIAKGQDMILRLDPQGMKKVKQVYPNAVTIFIVPPDPETWLE
                     RLIKRGSDTDEDLRVRIETAREEIKSIPEFDYIVVNDQLDKAVEDVLTILRAEHQRTT
                     RIKVEHN"
     gene            1502..>1700
                     /locus_tag="PKV59_09315"
     CDS             1502..>1700
                     /locus_tag="PKV59_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46989.1"
                     /translation="MNRLAVDSAAFPEEHRFNSDLFNSEPEKIPAEPDTLSEEDRQEA
                     SRRVSVRIPNAKPIVTWILCGV"
BASE COUNT          506 a          376 c          368 g          450 t
ORIGIN      
        1 tccgttaatc ggttatgcag ccggcgcggc tttggtaaaa gaagctgttg aaaagaaggt
       61 gccgattcgc cggttagccc atgagaaagc cgaagccgga ctgttaaaac gctcggacaa
      121 cggacagccc atctcccccg ccgaaatcga tcacgctttt tcggacttat tgcggatgac
      181 acaaggcgga ttcgtccggt aaacgaccgg aaaataggga caaaaattaa tcgatatcca
      241 tatcggcgaa atcttgatcg atcgagtcga ttgaatcgcc tctttccaat cgctcagcca
      301 cttcatcaaa ttcaggtgcc atcggctccc cgctctgatc tttgaacgag cgcatcattt
      361 tacccaattc gcgaggatca tcatacggtt cgttgaaact gtcgctatcc gcttcctgaa
      421 ccattcggaa ctggtcgctt tgtacgacag acaccggctg aatggcacgt tcgacgaatg
      481 tcgatcggca aaatgggcaa atcacatcca tctttccgta atcctcatag ctgattcgaa
      541 tttcaaattt ttttccgcaa ttcttacaaa aatagtcata aagcggcata ggatcgtcct
      601 ctgatcaatt ttttcaaaaa aaattggttt tcatcgcata tcagtcatca gaaataaaac
      661 aggattttca tcaaattctt ctttcaatta taatcttgta agaaacatga aaatattcga
      721 caaaaatgtt tctgacggtt ccggatactc tcatatccgg aaaaattttt tccgattgtc
      781 ggaaaggttt ttcttaatcg agaaaaaaat aatggaaaac tatggaatgg ttttgggatg
      841 aattagcaaa aaatcatcag gacattgcag acatggaatt atcactgcca tcattcaaaa
      901 tcttaaaatc cgatccgatg attgtgatta tttccggtct ctccggatcc gggaaagatt
      961 cggttatcaa tgcccttcgc aataattcaa aattgaattt tcattttgtc gtgacctgca
     1021 atactcgaaa acaacgcgat gatgaaattg acggagttca ttatcatttc atcagccgcg
     1081 aaaagttctt ggatatgatt cgtaagaatg agatgattga gtattcgcct gtatataatg
     1141 actataaagg ggtccctaag tttgaaatag aaaagggcat tgccaaaggg caggatatga
     1201 tattacgctt ggatccgcag ggcatgaaaa aagtcaaaca ggtttatccg aatgccgtga
     1261 ccatattcat cgtccctccc gatcccgaaa catggttaga gaggctcatc aagcggggaa
     1321 gcgataccga tgaagatctc cgagtccgta ttgagactgc gcgcgaggag attaaatcga
     1381 ttccggaatt cgattatatc gttgtcaatg accagttgga taaagcggtc gaagatgttc
     1441 taaccatctt gcgtgcggag catcaacgaa caacccgaat caaagtggag cataactgat
     1501 tgtgaataga ctcgccgtcg attccgccgc atttcccgaa gagcatcgat ttaactcgga
     1561 cctgttcaac tccgaacccg aaaagattcc ggcggaaccg gatactcttt cggaagaaga
     1621 cagacaggaa gcatcccgaa gagtttccgt tcgcatcccg aatgcaaagc cgatcgttac
     1681 ttggatttta tgcggtgtga
//
LOCUS       DAOMIV010000482         1699 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_83152_length_1699_cov_3.926399, whole genome shotgun sequence.
ACCESSION   DAOMIV010000482 DAOMIV010000000
VERSION     DAOMIV010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83152_length_1699_cov_3.926399"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="PKV59_09320"
     CDS             complement(<1..1686)
                     /locus_tag="PKV59_09320"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HQP46990.1"
                     /translation="MGTEDFFGLISNTAGGYCFYRDARLRRLTRYRYNNVPADSGGRY
                     LYLRDRQTGDYWSPTWQPVRSTLDHYSCRHGLGYTVIASEKNGIGFSGCYFIPIGENL
                     EIWDITITNRRKEEADLSLFSFIEFCLWDAQDDATNFQRNYNIAEVEVEGSTIFHKTE
                     YRERRNHFAFFHCSDPIAGFDTQREAFLGRYRGFDQPQAVEQGVSFNSIATSWQPIGS
                     HQVQFHLSTGECRRIIFLLGYHENPRGQKFDPPESNTLNKKTVYPIIREWEHPERVDR
                     AFSDLKSYWKGILQKFSVRTPVNHVDRMINTWNAYQCMITFNLSRSASYFESGIGRGM
                     GFRDSNQDLLGFMHMDPDRARQRILDLAATQMENGGAYHQYQPLTKRGNNDIGSGFND
                     DPLWLVLSTSAYLRETGDYSILEESIPFENRPGSEQPLSEHLDRCISYTIERIGPHGL
                     PLIGRADWNDCLNLNCFSDSPGQSFQTTTNREGKNAESVFIAGLFVLAANAYADILDI
                     LQQTEKSSRIRSYANEMKKVADTAGWDGRWFLRAYDDQGAAVGSHRCTEGKIFI"
BASE COUNT          481 a          412 c          348 g          458 t
ORIGIN      
        1 gataaagatt tttccttcgg tacaacgatg agagccgact gcagcgcctt gatcgtcata
       61 cgcccgtaaa aaccaccgac catcccatcc cgccgtatcc gcaacttttt tcatctcatt
      121 cgcataacta cgaatccgag atgatttttc tgtttgttgt aatatatcca aaatatccgc
      181 gtaagcattg gcagccaaaa caaataaacc ggcaataaat accgattcgg catttttacc
      241 ttcccgattg gtggtggtct gaaaagattg cccgggcgaa tctgaaaagc aattcagatt
      301 caaacagtca ttccaatccg ctctgccaat aagagggagt ccgtggggac ctatccgttc
      361 tatcgtataa gaaatgcagc ggtctaaatg ctccgacaga ggttgttccg atccaggtcg
      421 gttctcaaac ggtatggatt cttccaatat agaataatca ccggtttcgc gcaagtatgc
      481 ggatgtactc aataccaacc ataatggatc gtcattaaag cctgatccga tatcattatt
      541 tcctcgcttg gtaagcggtt gatattgatg atatgcccct ccgttttcca tttgcgtcgc
      601 ggcaagatca agtatccgtt gccgagcgcg gtcaggatcc atatgcataa atcccaatag
      661 atcttgattt gaatcacgaa aacccattcc ccgcccgatc ccgctttcaa aataggatgc
      721 cgagcgagac aggttgaatg tgatcataca ctgatatgca ttccaggtat ttatcatacg
      781 atctacatgg ttcaccggtg tccggacaga aaacttttgc agaatacctt tccaatagga
      841 tttaaggtcc gaaaatgccc gatcaacccg ctccggatgt tcccattcac gaataatggg
      901 ataaacggtt tttttattga gcgtattcga ttcgggagga tcaaattttt gacctcgggg
      961 attttcatga tatcccagca aaaaaatgat ccggcggcat tcaccggtgc tgagatggaa
     1021 ttgtacttgg tgagagccga tcggctgcca ggacgtcgca atcgaattga aagaaactcc
     1081 ttgttcaacc gcctgaggtt gatcaaagcc acgatagcgt ccgagaaacg cttctcgttg
     1141 cgtgtcaaaa cccgctatcg gatccgaaca gtggaaaaat gcaaaatgat tccgccgttc
     1201 tcgatattcg gttttatgga aaatcgtact cccttcaact tccacttccg cgatattgta
     1261 attacgctgg aaattggttg catcatcttg tgcatcccat aagcagaatt ctataaatga
     1321 aaataaagat aaatccgctt cttcttttcg ccgatttgtg atcgtaatgt cccagatttc
     1381 aagattttca ccgatcggaa taaaatagca tcccgaaaag ccaattccgt ttttctctga
     1441 agcgataacg gtataaccca atccgtgccg gcagctgtag tgatccaaag ttgatcgaac
     1501 cggttgccaa gtcggcgacc aatagtcgcc tgtttgtcga tcccgcagat aaagatatct
     1561 tcctccgcta tccgcaggaa cattattata gcgataacgg gtcagtcgac gtaaacgtgc
     1621 atctcgataa aaacagtatc caccggccgt atttgaaatc aatccaaaaa aatcttctgt
     1681 ccccaaataa ttaatccaa
//
LOCUS       DAOMIV010000483         1696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_83377_length_1696_cov_3.200488, whole genome shotgun sequence.
ACCESSION   DAOMIV010000483 DAOMIV010000000
VERSION     DAOMIV010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83377_length_1696_cov_3.200488"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PKV59_09325"
     CDS             complement(<1..622)
                     /locus_tag="PKV59_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQP46991.1"
                     /translation="MKKLLVTGASGLLGMNLALGLSKKYQIFGVANHLTLKGIPFEMI
                     QRDFFRKDVAAELIDTVQPDAVIHCAAMANVDACEKNPEAAMELNGEFPGRLAGETAK
                     RHIPMIHISTDAVFDGKERKENGYTENDLPHPINVYARSKLLGEQKVFEANPDAVVAR
                     VNFYGWSVSGNRSLSEFFYANLKSGQRVFGFTDVFFCPLYVWDLAEI"
     gene            complement(625..1629)
                     /gene="pseB"
                     /locus_tag="PKV59_09330"
     CDS             complement(625..1629)
                     /gene="pseB"
                     /locus_tag="PKV59_09330"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HQP46992.1"
                     /translation="MDWTDKVVLITGGTGSFGKKFIQVMLKEYHPEKIIIYSRDELKQ
                     YEMQNSGINDPRLRYFIGDVRDLDRMKRAFQGVDIVVHAAALKQVPACEYNPFEAVKT
                     NILGTENVIDAALDMGVKKVLAISTDKAVNPVNLYGVTKLAAEKLVIQSNSYAGGRET
                     RLSCVRYGNVVGSRGSVVPVFLKQRATGRLTVTDERMTRFWISLEQGVHFVIRCIEQM
                     HGGEVFVPKIPSMKVKDLAAAVAPEAEITYTGIRPGEKLHEVLISEDEARNTVEFDDM
                     FIVQPSGTTWFGRDWEKLGKKLPDDYHYSSNNNTEWLDLEGIKKMIAPFMESYDQEEF
                     "
BASE COUNT          439 a          447 c          341 g          469 t
ORIGIN      
        1 atatttccgc caaatcccaa acatagagag gacagaaaaa gacatcggta aacccgaaaa
       61 cccgctgtcc ggatttgaga tttgcataaa aaaactcgct taagctgcga tttccggaaa
      121 cactccagcc gtaaaaattg acacgagcga cgacggcatc ggggttcgcc tcgaaaacct
      181 tttgttctcc caataatttg gaccgggcat aaacattgat cggatgagga agatcgtttt
      241 cggtatatcc gttttccttt cgctctttgc cgtcgaaaac cgcatccgtg gaaatatgaa
      301 tcatcggaat atgccgtttt gcggtttctc cggccaatct gccgggaaat tccccattca
      361 gttccattgc tgcttccgga ttcttttcac aggcgtccac attggccatc gcagcacaat
      421 gaatgacggc atcgggttga acggtatcga tcaattcagc ggcaacatct ttccgaaaaa
      481 aatccctttg aatcatttcg aagggaattc ctttcagcgt caaatgattc gcaactccga
      541 aaatctgata ttttttgctg agccccaacg ccagattcat ccccagcaag ccggatgctc
      601 ccgtaaccaa caattttttc atcgctaaaa ttcttcttga tcgtaagatt ccataaaggg
      661 cgcgatcatt tttttaatcc cttccaaatc gagccattcg gtattgttat tactggaata
      721 atgatagtca tccggcaatt ttttgcccaa tttttcccaa tccctgccga accaagtcgt
      781 gcccgaaggt tggacgataa acatatcatc gaattcaacc gtatttcgtg cttcgtcttc
      841 ggaaatcaaa acctcatgaa gtttttctcc gggacgaatg ccggtgtaag taatttcggc
      901 ttccggagcg acggcggcgg ctaaatcttt taccttcata ctgggaattt tcggcacgaa
      961 cacttccccg ccgtgcattt gttcgataca tcggatgaca aaatgaacgc cttgttccag
     1021 agaaatccaa aaccgggtca tccgttcatc ggtaaccgtt aatcgtcccg ttgcccgctg
     1081 tttcaggaaa accggcacaa ccgaaccgcg gctgccgacc acatttccgt atcggacaca
     1141 agaaaggcgc gtttcccgcc cgccggcata tgagttgctt tgaatgacca atttttcggc
     1201 ggctaatttt gtcacgccat aaaggttaac cggattcacc gctttgtccg tactgatcgc
     1261 caggactttt tttacgccca tatccaatgc ggcatcaatc acattttccg tacccaatat
     1321 atttgttttg accgcctcaa acggattgta ttcgcatgcc gggacctgtt tcaaagcggc
     1381 ggcatgaacg actatatcaa ccccttgaaa agcccgtttc atacgatcca aatcccgaac
     1441 atcaccgata aaatatcgca agcgcgggtc attgatcccg gaattctgca tttcatactg
     1501 tttaagttca tcccggctgt aaataatgat tttttccgga tgatattcct tcagcatgac
     1561 ctggatgaat ttctttccga aagatccggt ccctccggta atcagtacaa ctttatctgt
     1621 ccaatccatg aatttcctcc gcgggaaaaa tcttttgccg agaaagcctc ggataatttt
     1681 cccttttagg ggcgct
//
LOCUS       DAOMIV010000484         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_83916_length_1689_cov_3.569155, whole genome shotgun sequence.
ACCESSION   DAOMIV010000484 DAOMIV010000000
VERSION     DAOMIV010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83916_length_1689_cov_3.569155"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PKV59_09335"
     CDS             <1..1001
                     /locus_tag="PKV59_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HQP46993.1"
                     /translation="PKCQNELIEEIAKVNPNTVVLLHNGSPVEMPWADHVPAILECYL
                     GGQAVGGAEVDVLFGKVNPSGKLAESFPHQLEDNPSYLNFPGEGDVVEYREGVYVGYR
                     YYDKKKMAVRFPFGHGLSYTTFEYSNLKLSQKHLNQGESLDVSVNITNNGKVKGKEAV
                     QLYVQSTHTGVPRPIKELKEFAKVELEPGETKTIHFTLPPRAFAYYEVKIKNWYVEGG
                     SYDILIAASSRDIRLSDSIEIAAGDPIPFKVTPDTLIMDLLKLPKAAELIKPFMEAAA
                     GHLSANAESNELGDATKAMRESMIMGLPLHGIRSWIGDTFTDEAMWQIINTLNQEE"
     gene            1098..>1689
                     /locus_tag="PKV59_09340"
     CDS             1098..>1689
                     /locus_tag="PKV59_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQP46994.1"
                     /translation="MKKFALLFTLVLIFTAVFTVAAEDFPREESLTTNGQQWGAPANF
                     NPYALSNIAWPVADTRRIPIYESLYMYNMLTNENEPLLADGPIVWEDDYNIIVKIKPV
                     AHWSDGEPLTADDVVFTFNSLDPDVIGYQTQWSDVWSYLEKVEKVDDLTVRFTIPAEP
                     YNVHMLPSYLADTRIFPKHIWEPLFEGELKETTAKQE"
BASE COUNT          507 a          355 c          390 g          437 t
ORIGIN      
        1 tgccgaaatg ccagaatgaa ttaattgaag aaattgcaaa agtcaacccc aatacagtgg
       61 tgcttttaca taatggttcg ccggtcgaaa tgccatgggc tgaccatgta ccagcaattt
      121 tagaatgcta tttgggaggt caggcagttg gaggagcgga agtggatgtt ctattcggta
      181 aggttaatcc gagcggaaaa ttagctgaat cctttccaca tcagttagaa gataacccgt
      241 cctacctgaa tttcccaggc gaaggagacg tcgtagaata tcgcgaaggc gtctatgtcg
      301 gataccgata ctatgacaag aaaaaaatgg cggtacgatt tccgtttggt cacggtttaa
      361 gctataccac ttttgaatat tcgaatctga agctcagcca aaagcacctc aatcaaggag
      421 aatctctcga tgtttcggtg aatatcacga ataacggaaa agtgaaaggc aaggaagcgg
      481 ttcagctcta cgtgcaaagc acacataccg gcgttccacg cccgataaaa gaattgaaag
      541 agtttgcaaa ggtcgaattg gaaccagggg aaacaaaaac aattcatttc accttgcccc
      601 cccgagcctt cgcttattat gaagtcaaaa ttaagaattg gtatgtcgaa ggcggatcct
      661 atgatattct gatcgcagca tcttctcggg atattcgctt atcggattcg attgaaattg
      721 ctgccgggga tcctattccg tttaaagtta cccccgatac tcttattatg gatttactca
      781 aactgccaaa agcagccgaa ttaattaaac cgtttatgga agccgcagcc ggtcacctca
      841 gtgcaaacgc ggaatcgaat gagttgggtg atgcgacaaa agcaatgcgg gaaagtatga
      901 ttatgggatt accattgcat gggattcgat cctggatcgg tgacactttc accgacgaag
      961 caatgtggca aattatcaat accttgaacc aagaagaata ggaggaagcc aatcagagaa
     1021 acacagtata tctttttacg gaggcaggga atttatcctg cgatattcag cttactaatt
     1081 tataaggaga gattgaaatg aaaaaatttg ctttattgtt tacgctcgtg ctaatcttta
     1141 cagccgtatt caccgtcgct gcagaagatt ttccgcgcga ggaatcttta acgacaaatg
     1201 gccagcaatg gggagcaccc gcgaacttta acccgtacgc tttgtccaac attgcctggc
     1261 cagttgccga taccagacga atccccattt atgaatcatt gtatatgtac aatatgctga
     1321 caaatgagaa tgaaccatta ttagcggatg gtcctatcgt ctgggaagat gattataaca
     1381 ttattgttaa gatcaaaccc gtcgcacatt ggtcagatgg tgagccatta accgcggatg
     1441 atgtggtgtt tactttcaat tcgcttgatc ccgatgtgat cggatatcag actcaatggt
     1501 ccgatgtatg gtcctatctt gaaaaagtgg aaaaagtgga tgatctgacg gttcgcttta
     1561 caatccccgc cgagccatac aatgtgcata tgctccccag ctatttggcg gatacccgta
     1621 ttttcccgaa gcacatttgg gagcccttat ttgaaggtga actgaaagaa accaccgcga
     1681 aacaggaat
//
LOCUS       DAOMIV010000485         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_84249_length_1685_cov_3.458282, whole genome shotgun sequence.
ACCESSION   DAOMIV010000485 DAOMIV010000000
VERSION     DAOMIV010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84249_length_1685_cov_3.458282"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..1639)
                     /locus_tag="PKV59_09345"
     CDS             complement(422..1639)
                     /locus_tag="PKV59_09345"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HQP46995.1"
                     /translation="MVREQFQVKINSFTLNVTQTAVDAVRKKQITKTGCRVYSDGYIG
                     ISGTFGEADEQTWLDAEKNLMKKIPYPYPPSAGKTRIRNTKKACGSQTDFLKQSEEFL
                     ARIRKDFPDFICSNKLMSVESEVSIHNDLGLDYCNSDQSIVFGLVVKHIKSINVFDTI
                     YSFEDRDPDFESVYEDLSSQLTAFGNPMEQKISGKIPVVIGFQDISGKFIESLNGEAL
                     GRGVSIFGDKIGKKVFNDDFSLIVDRGIEPIHASFFDVEGSVLESDQTALIEKGQIRC
                     GYADKKTADKYGMRNTAAAGGEYNSVPALSVPPLSVLPSQKTLAEILNGRDAIFAAVL
                     SGGDFTNEGQFASPVQMAYSIRDGKLVGKLPELTISGNIYDLFGNDYFGQSGDKPFCG
                     ERYLVLNMDVNQY"
BASE COUNT          535 a          355 c          291 g          504 t
ORIGIN      
        1 acataaatat ttcaaaagtt gttatagccc gaaaaataaa aagagttgta ccaaattgat
       61 tataccttta tcaacgaaac agaaagaatg gcttggaatc acataccctc gggaataaat
      121 gcttacgatt taatgacccg agggatcgtt agaaattttt acgaaagttc tattattgct
      181 gattattaat ctcaggaatg aggcaaaaac gagaaaaaaa gatagggaat aagctaaacc
      241 ttaatttgac ataaaagcaa acaacaataa aaaacgcaaa tctatagaat cttctttatt
      301 gcagcaaaaa taatcgttta ataaaaattt gagggaatcg caattgccga tagatctgta
      361 aaaaaaaaca aaaaacaggc taagcacttt aacctgtttt tatattttcg gttcattttt
      421 attaatattg attaacatcc atatttaata ccagataacg ttcgccgcaa aaaggtttat
      481 caccactttg gccgaaataa tcatttccga ataaatcata gatattcccg gaaattgtta
      541 attcgggtaa tttcccgact aatttaccgt cccgaatgga atatgccatt tggacaggtg
      601 aagcaaattg tccttcgttg gtaaagtctc ctccgcttaa aaccgctgca aagatggcgt
      661 ctcgaccatt caatatctca gccaaagttt tctgtgacgg taaaaccgaa agagggggta
      721 cactcaatgc cgggacactg ttatattcgc cccccgccgc agctgtattc cgcattccgt
      781 atttatcggc agtcttttta tcggcatatc cacatcgtat ttgtcccttc tcaatcagag
      841 ccgtttgatc cgactcaagg acgctacctt caacatcaaa aaacgaagca tggatcggtt
      901 caattccccg gtcaactatt aaagaaaaat catcgttaaa aacctttttc ccgattttat
      961 cgccaaatat ggatactcct cttccgagtg cctcaccgtt gagtgattcg ataaatttac
     1021 cgctgatgtc ttgaaaaccg attacaaccg ggatttttcc gctgattttt tgctccatcg
     1081 gattcccaaa agcggtcaat tgagaagata gatcttcata gacggattca aaatcggggt
     1141 cgcgatcttc aaacgaataa atcgtatcaa atacattaat cgattttata tgttttacca
     1201 ccaagccgaa aacaatcgat tgatccgaat tgcaataatc caatccgaga tcattatgaa
     1261 ttgagacttc ggattcaacc gacattaact tattactgca gataaaatcc ggaaaatcct
     1321 ttcgtattcg cgctaaaaat tcttcactct gtttgagaaa atccgtctga gaaccgcaag
     1381 cttttttggt atttcgaatt cgtgtctttc cggctgaggg aggatatgga taagggattt
     1441 tcttcatcaa gtttttctcg gcatcaagcc aagtttgctc atcggcttcc ccgaaagtac
     1501 ccgagatacc aatgtaaccg tccgaataga ctctgcaacc ggtttttgta atttgtttct
     1561 ttcgcaccgc atccacagcg gtttgagtga catttaaggt aaaactgttg attttcacct
     1621 gaaattgttc gcgtaccata tcttttcctt tttactgaac attaattcca ttgacccgga
     1681 catac
//
LOCUS       DAOMIV010000486         1685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_84263_length_1685_cov_2.979141, whole genome shotgun sequence.
ACCESSION   DAOMIV010000486 DAOMIV010000000
VERSION     DAOMIV010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84263_length_1685_cov_2.979141"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PKV59_09350"
     CDS             complement(<1..260)
                     /locus_tag="PKV59_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQP46996.1"
                     /translation="MLNVGIIGCGKIAEVRHAPEYLENPSCNIVGFFDEIPARAESYA
                     EKFGGKAFNTIQELLNSGVDAVSVCTSNTTHAEISIQALNAGV"
     gene            complement(275..1264)
                     /locus_tag="PKV59_09355"
     CDS             complement(275..1264)
                     /locus_tag="PKV59_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQP46997.1"
                     /translation="MTSVIQKNSLKSFLKKLVTQYSYWLSFFMLLILAVAVNTSFLRW
                     SNISNIFVQTATTGIIALGMTMIISAGQIDISVGAQVAIISGLGIVVLNRTNNILIMF
                     LFCMGLGFIIGLCNGLLVSKGRMPAMIATLATQSICRSLINQLGQGGPFTVSDVNYGS
                     FRKVAAGYVPLGNFRIPYPMLMFIGFSLLFGLIMSRTKLGKHIYAVGSNQTSARLAGV
                     NVEKVQNLVFIITGLLCGFSGWLYASRVMAVAAASAGNGYEMEVIASVAIGGTAMTGG
                     RGQVIGTFLGALMFKIINNILTMANVPTFLNGAISGLIIIVAVLMQNVQNKSK"
     gene            complement(1233..>1685)
                     /locus_tag="PKV59_09360"
     CDS             complement(1233..>1685)
                     /locus_tag="PKV59_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP46998.1"
                     /translation="HKGLLIQPKDQKERFKRQQESLSIKTDDPKNPITSLSGGNQQKI
                     ILGRWLEADAEIILFDNPTQGIDVGTKFEIYHLILNLAKQGKAIIVFSSEFPEIFKVA
                     DSCIVLFKGNINARLSREEITERNIMYYSTGANLEAEKNDKCDSKKLS"
BASE COUNT          453 a          402 c          366 g          464 t
ORIGIN      
        1 acaccggcat tgagcgcctg gatggaaatt tccgcatgtg tggtattgga agtgcatacg
       61 ctcaccgcat ccacgccgct gtttaacaac tcttggatcg tattaaaggc ttttccgccg
      121 aatttttccg catagctttc cgcacgcgca ggaatttcgt caaaaaagcc gacgatattg
      181 caactggggt tttccaaata ttccggtgcg tgacgaactt cggctatttt tccgcatccg
      241 ataatcccga catttaacat atggattatt cctcctattt gcttttgttc tgaacgttct
      301 gcatcagaac cgctacgata atgatcaaac cgctgattgc gccgttcagg aaagtaggta
      361 catttgccat ggtcaaaatg ttattgatga ttttaaacat caatgcaccc aaaaatgttc
      421 cgatcacctg tccgcgtccg ccggtcattg ccgttccgcc gatggcgacc gaagcgatta
      481 cttccatttc atatccgttg cctgcgctgg ctgccgccac cgccatcacg cgggatgcgt
      541 aaagccagcc cgaaaagcca caaagaagac cggttatgat aaataccaga ttctggactt
      601 tttccacgtt gacgccggcc aacctggccg aagtctgatt actgcctacg gcataaatat
      661 gtttgcccag ttttgtccgg ctcatgatta atccgaacag cagcgaaaag ccgatgaaca
      721 ttaacatggg ataaggaatc cgaaaattgc ccagcggaac atagccggcg gcgacttttc
      781 gaaaacttcc gtaattgaca tcggacaccg tgaaaggtcc gccctgacca agctggttaa
      841 tcaacgaacg gcagatgctt tgtgtcgcca gtgtggcgat catagccggc atacgacctt
      901 ttgaaaccaa taaaccgttg cacagaccga tgatgaagcc caatcccata caaaatagga
      961 acatgataag aatattattg gtcctgttta aaacgacgat gcccaaaccg gagataatcg
     1021 cgacctgagc gcctacggag atatcaatct ggccggcaga aataatcatc gtcatcccca
     1081 atgcaatgat tccggttgtc gctgtttgaa cgaaaatgtt gctgatattg ctccaccgca
     1141 ggaagctggt atttaccgcg accgccagaa tcaagagcat gaagaaagac aaccagtagc
     1201 tgtattgggt aacgagtttc ttcaggaaag atttaagaga gtttttttga atcacacttg
     1261 tcattttttt ctgcctccaa atttgctccg gttgaatagt acataatatt tctttcggtt
     1321 atttcttcac gtgaaagacg ggcattgata tttcctttaa aaagaacgat acaggaatcg
     1381 gccactttga aaatctcggg aaactccgaa ctgaatacga taattgcttt cccttgtttc
     1441 gctaaattga gtatcaagtg atatatttcg aattttgtcc cgacatcgat cccttgcgtc
     1501 ggattatcaa aaaggatgat ttctgcatca gcttccaacc aacgtccgag aatgatcttt
     1561 tgctgatttc ccccagaaag agaggtgatc ggatttttgg gatcatccgt tttaatggat
     1621 aatgattcct gttggcgttt aaaccgttcc ttttggtctt tgggttggat caatagccct
     1681 ttatg
//
LOCUS       DAOMIV010000487         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_86061_length_1663_cov_3.714552, whole genome shotgun sequence.
ACCESSION   DAOMIV010000487 DAOMIV010000000
VERSION     DAOMIV010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86061_length_1663_cov_3.714552"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PKV59_09365"
     CDS             complement(<1..930)
                     /locus_tag="PKV59_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQP46999.1"
                     /translation="MNKEVLDGLFRPKSVAVIGASGTPGKIGYSVVEALINGKYEGKI
                     YPINLKLTEILGRKAYPTIGDVPEEVDLAVITIPAKFVLGAVEECGKKGVKGISIITS
                     GFSEIGDHETENAIVETARRYGMRILGPNIIGYISNSDKLNASFAPMLPLPGNASLIS
                     QSGALLIALEAGTYLRGVGFDKLFSLGNMADIDFADVIEWLNEDENTKCISLYIEGLK
                     DGRRFMEKCKESTKPIIVLKSGVSAHGAAAAASHTGSLAGAAKVYSAAFEQSGIIQAA
                     NLDNLFDLTEAFELQPPMLGDHLLVITNGGGVGV"
     gene            complement(1072..>1663)
                     /locus_tag="PKV59_09370"
     CDS             complement(1072..>1663)
                     /locus_tag="PKV59_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47000.1"
                     /translation="DIETARNSPHIEMLKKDGLDVLLMVDPFDPFVVTELEKYQDFPL
                     VNAATEKAEAKKDDKDQEEKTSEEPKEPSAGEYADLIKMFKEELGDKVSDVRVTGRLV
                     ESPARMVNAEGALSPEMQKMYNLMQQDFQKPKKILEINPKHPLIAKLEKLEDKQLANQ
                     IIDQIYDGAMLMDGEKTDQYQMLNRIQNILINLLNK"
BASE COUNT          442 a          384 c          312 g          525 t
ORIGIN      
        1 aacgcccaca ccgccgccgt tggtgataac taacaggtga tcaccgagca tggggggttg
       61 caattcaaaa gcttctgtga ggtcaaacag attatccaaa ttggcagcct gaataatgcc
      121 cgattgttca aatgccgcac tgtagacttt tgcggcgccg gcaagcgacc ccgtatgaga
      181 ggctgcggct gcggcaccat gtgcggaaac gccggatttc aaaacgatga tcggttttgt
      241 ggattctttg catttttcca tgaatcgacg gccgtctttt aatccttcga tatagagaga
      301 aatgcatttc gtattttcat cctcattcaa ccattcgatc acatcagcga aatcaatatc
      361 cgccatattt ccgagggaga ataatttatc aaaaccgact ccccgtaaat atgtcccggc
      421 ttccaaagca attaaaagcg ctcccgattg ggaaattaat gaagcgttac ccggcagtgg
      481 taacatcgga gcaaaagaag cgttcagttt atccgaatta gaaatatagc cgataatatt
      541 cggacccaaa attcgcattc catatcgacg ggcagtttct acgatggcat tttccgtctc
      601 atgatcgccg atttcactga aacctgaagt gatgatactg atccctttta cacctttttt
      661 accgcattct tcaaccgctc ccaaaacaaa tttggccgga atggtgataa cagcaagatc
      721 aacttcttcc ggaacatcgc cgattgtcgg atatgctttc cgtccgagaa tttcagtcaa
      781 ttttagattg atcggataaa tcttgccttc atacttacca ttaattaagg cttcgacaac
      841 ggaatatcca atttttccgg gagttccgga agcccctatg acagcaacac tttttggtct
      901 gaagaggccg tccaacactt ctttattcac tttttacctc gcttgaattt aaaaaaatta
      961 gattattatg aaaattcgcc ataatttcag attttctgaa ttattttatc acatatataa
     1021 gattaaaaac aaaaaaggag aaaattcttt ctcctttttt atcaatcgat tctatttatt
     1081 caataaattt atcagaatgt tctgaatcct gtttaacatt tgatattgat ctgttttttc
     1141 accgtccatc aacatcgctc cgtcataaat ttggtcaata atttgattgg cgagttgttt
     1201 atcttccaat ttctccaact tggctatcaa aggatgtttc ggattaattt caagtatttt
     1261 tttcggtttt tggaaatcct gctgcatcag gttatacatt ttttgcattt ccggagaaag
     1321 tgcaccctcg gcgttgacca tccgagccgg tgactccaca agtctgccgg taactcgcac
     1381 atccgagact ttgtcgccga gctcctcctt gaacatcttg attaaatccg catattcgcc
     1441 ggcggaaggt tccttaggtt cttccgatgt tttttcttct tggtctttgt catctttttt
     1501 cgcctccgct ttctctgtgg cggcatttac cagcggaaag tcctgatatt tttccaactc
     1561 ggtgacaaca aacggatcaa agggatctac cattaaaagt acatccaagc catctttttt
     1621 gagcatttca atatgagggc tgttccttgc cgtttcgata tct
//
LOCUS       DAOMIV010000488         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_86071_length_1663_cov_3.208333, whole genome shotgun sequence.
ACCESSION   DAOMIV010000488 DAOMIV010000000
VERSION     DAOMIV010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86071_length_1663_cov_3.208333"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PKV59_09375"
     CDS             <1..1267
                     /locus_tag="PKV59_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQP47001.1"
                     /translation="FMGLGHVLYLKQYVKGILYALIEVLFLLFTPTVYKKIVGLITLG
                     DPHPELPIKQRDHSMFMMIDGILILAVVFIFILLYYLSVQNALSDYKDYCATGIKAKN
                     KNIFAELGNKSFPIVGLMPMILLVLFFVVVPLIFSACVAFTNYSAPKNIPPNNTVDWV
                     GLDNFRALFGGDATWSGALGRVASWTLVWAFFATLTCYAGGMIMAVILQESKIKIKPF
                     FRSIFILPYAVPTVVSMLVWQNLLNGSFGTVNRSLIALGFLDKSTVIPWLSDVTLARF
                     VVILINLWAGFPYFMLLTMGTMTSISNEQYEAAGMDGCTKTQAFRYITLPQVLYQTMP
                     LIIMSFTYNINNFGAIFFLTGGNPIVADSTITSARGTDIMVTWIYKLTITLLKYNYAS
                     VLAVCIFVVLAPFAIYNFRRTKSYKEGEL"
     gene            1267..>1663
                     /locus_tag="PKV59_09380"
     CDS             1267..>1663
                     /locus_tag="PKV59_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47002.1"
                     /translation="MVETQFDKKMKRERIISKINTGLVLIFLILVTLIVLYPLVYLVS
                     AAFSPGNSISSLNIIPFGDGFTIEHYRRLFRETNYLNWFKNTLIIAAGTSISTVAVCA
                     IAAYVFSRFRFPFKKGMMLLLLILQIFPSF"
BASE COUNT          403 a          366 c          359 g          535 t
ORIGIN      
        1 ttttatggga ttgggacatg ttctgtattt gaaacagtat gtcaaaggaa ttctctatgc
       61 actgatagaa gttctttttt tgctttttac cccgaccgtt tataaaaaga ttgtcggctt
      121 aatcacgctg ggagatcctc atccggaatt gccgattaaa cagcgcgatc attccatgtt
      181 tatgatgatc gatggcattc tgattctggc ggtcgtattt atttttattc tgctctatta
      241 cctatccgtc caaaatgcat tatccgatta caaagattat tgcgccactg gcatcaaagc
      301 gaaaaataag aatatctttg ccgaattggg aaataaatct tttccgatcg tcggtttgat
      361 gccgatgatc ctgcttgtct tattctttgt cgtcgttccg ttgatctttt cagcctgtgt
      421 ggcttttaca aattattctg ccccgaaaaa cattccgccc aataataccg ttgattgggt
      481 gggactcgat aattttcgtg cccttttcgg cggggatgcc acctggtcag gagcgctcgg
      541 tcgggttgcc tcatggacat tagtttgggc attttttgca acgttaacct gttacgccgg
      601 cggaatgatc atggcggtga tattacagga atcaaagatt aaaatcaaac ctttttttcg
      661 ttccattttt attttacctt acgcggttcc gacggttgtc agcatgctgg tctggcaaaa
      721 cctgttgaat ggatcgttcg ggacagtaaa tcggtcctta atcgccttgg gctttctgga
      781 taaatcgacg gtcatcccct ggttgagcga cgtgaccctc gctcgctttg tggttatctt
      841 gatcaatctt tgggcgggtt tcccttattt tatgctgctg acaatgggaa caatgacatc
      901 catcagcaat gaacaatacg aagcggccgg catggacggc tgcaccaaaa cacaggcttt
      961 tcgttacatt acgctgccgc aggttttgta tcaaaccatg ccactcataa ttatgtcatt
     1021 tacctacaat atcaacaatt ttggggctat cttcttcttg accggaggaa acccaatcgt
     1081 ggccgattcc accatcacaa gcgcccgtgg cacagatatt atggtgacct ggatctacaa
     1141 attaacaatt accttactga aatacaatta cgcttccgtg ttggcggtat gcatttttgt
     1201 ggtgctcgct ccttttgcaa tttataactt cagaaggaca aaatcataca aggaaggaga
     1261 gctgtgatgg tagagactca atttgacaag aaaatgaaac gggaacggat aatcagtaaa
     1321 atcaataccg gcttggtatt aatcttcctg attctggtga cattaatcgt gctgtatcct
     1381 ctggtttatt tggtcagtgc ggcgttctca ccgggaaatt ccatctcgtc cctcaatatt
     1441 atcccgttcg gagacgggtt tacgatcgaa cactatcgcc ggctcttccg ggaaaccaat
     1501 tacctgaatt ggtttaaaaa tacactcatt atcgccgccg gaacttcaat ttcaacagtc
     1561 gcggtttgtg ccattgctgc ctatgtcttt tcacggtttc gttttccgtt taaaaaaggg
     1621 atgatgctgt tactgttgat tttgcagatt tttccgtctt ttg
//
LOCUS       DAOMIV010000489         1656 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_86614_length_1656_cov_3.796377, whole genome shotgun sequence.
ACCESSION   DAOMIV010000489 DAOMIV010000000
VERSION     DAOMIV010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86614_length_1656_cov_3.796377"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PKV59_09385"
     CDS             <1..493
                     /locus_tag="PKV59_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HQP47003.1"
                     /translation="LIDPIADYCKKSKEKGWTILALSDTHDPQDIEMKSFPSHCLAGS
                     DESEVVDEIKPYCDFILPKFTTGGFFEIQEASQENKSLDLRRYEEIHVIGCCTDLCVF
                     NFCLITQKHMEYEYHKKRISRIPDIIIHKDLVDTYDSEGHEADNLNRFFWEHMKLNGI
                     KVE"
     gene            493..>1656
                     /locus_tag="PKV59_09390"
     CDS             493..>1656
                     /locus_tag="PKV59_09390"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HQP47004.1"
                     /translation="MTAHNLREKYDSRNLSMLFDFYEMTMGNGYFKQGMKDKIVIFDL
                     FFRRIPDDGGFAIICGLEQVVQYIENLHFTEDELAYLTNKKLFDPDFIDYLRHFEFHC
                     DVWAIPEGTPVFPNEPLVRVRGPIIEAQLIETMLLVTINHQSLIATKTNRIVRTAKGK
                     TVLEFGTRRAQGYDAANYGARAAYIAGVAGSANNWADIHLGVPASGTMAHSWILSFDS
                     EYEAFKAYAKSYPDNCNLLVDTYNTLKSGVPNAIRVAHEVLEPMGKRLVGIRLDSGDI
                     AYLSKKARKMLDDEGLKDCKIFASNSLDEYIIRSLNVQGARVDVYGVGERLITASSEA
                     IFGGVYKLVGIYDENGNLIPKIKISDTFEKITNPSFKEIWRLYEIGDNHPLADV"
BASE COUNT          485 a          311 c          391 g          469 t
ORIGIN      
        1 tctgatcgat ccgatagccg attattgtaa aaaatcaaaa gaaaaaggtt ggaccattct
       61 tgctctttcg gatacgcatg atccacagga tattgaaatg aaatcgtttc cgagccattg
      121 tttggccggc agtgatgaat cggaagtggt cgatgaaatt aaaccttatt gtgattttat
      181 ccttccgaaa tttacgaccg gtggattttt tgaaatacag gaagcatctc aagaaaacaa
      241 atcgctggat ttacgtcgtt atgaggaaat tcatgtgatc gggtgttgta ccgacctttg
      301 tgtcttcaat ttttgtttga ttactcaaaa acacatggaa tacgaatatc ataaaaagcg
      361 gatctcaaga ataccggaca taatcattca caaggatttg gtcgatacct atgattcgga
      421 aggccacgag gcggataatc tgaatcggtt tttctgggaa catatgaaat taaatggaat
      481 aaaggttgaa taatgacagc acataatctt cgtgaaaaat acgattcaag aaatctgagt
      541 atgctttttg acttttatga aatgacaatg ggcaacggtt atttcaagca agggatgaaa
      601 gataaaatcg ttattttcga tttgtttttc cggcgaatac cggatgacgg aggctttgcc
      661 attatatgcg gcttggaaca agtcgtccaa tatattgaaa atttgcattt taccgaagat
      721 gagctggcat atctgacgaa taaaaagctc tttgatccgg attttattga ttatttgcgt
      781 catttcgaat ttcattgcga tgtgtgggcg atacccgaag gcacgcctgt tttccccaac
      841 gagcctttag tccgggttcg gggtccgata atcgaggctc aattaattga aacaatgtta
      901 ttggtaacga tcaatcatca atccttgatc gcgacaaaaa cgaatcggat tgttcgaact
      961 gccaagggga aaaccgttct tgaattcggc actcgccgag cccagggata tgatgccgcc
     1021 aattacggtg cgcgtgccgc ttatatcgcc ggcgtggcag gctcagcaaa taactgggcg
     1081 gatattcacc tcggcgttcc ggcaagcgga acgatggcac attcctggat tttgtctttc
     1141 gattcggaat atgaggcttt taaagcttat gcgaaaagct atccggataa ctgcaacctt
     1201 ttggtcgaca cctacaatac gctgaaatcg ggcgttccga atgccattcg tgtggcacat
     1261 gaagtactcg agccgatggg caaaaggttg gtcggaattc gcttggacag cggtgacatc
     1321 gcttatttgt ccaaaaaagc cagaaagatg ctggatgatg agggactgaa agattgtaaa
     1381 atatttgcca gcaattcgct ggacgaatat attatccgat cattaaatgt tcagggtgcc
     1441 cgtgtggatg tttatggtgt cggagaaaga ttgataaccg ccagcagcga agcgattttc
     1501 ggcggtgtct ataagctggt gggtatttat gacgaaaatg gaaacttgat tccaaaaatt
     1561 aaaatttccg atacttttga aaagattaca aacccttcct ttaaagagat atggcgctta
     1621 tacgaaatcg gcgataacca tcctttggcg gatgtc
//
LOCUS       DAOMIV010000490         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_86884_length_1653_cov_2.720275, whole genome shotgun sequence.
ACCESSION   DAOMIV010000490 DAOMIV010000000
VERSION     DAOMIV010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86884_length_1653_cov_2.720275"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1653
                     /gene="leuS"
                     /locus_tag="PKV59_09395"
     CDS             12..>1653
                     /gene="leuS"
                     /locus_tag="PKV59_09395"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQP47005.1"
                     /translation="MVNKLTKPEQAESVKAYIEQAKKQSDIQRESVDREKTGVFTGSY
                     AINPVNGARIPIWVADYVLMGYGTGAIMAVPAHDQRDFEFARKFGLQVIAVVQPDDMA
                     PLDGDTMKEAVPAHGRLINSGEMTGVSGEEAFDRVVDWLEKNHLGHKTVTYRLRDWLI
                     SRQRYWGAPIPMIHCPKCGVVPVPEDQLPVLLPDDVEWKPTGESPLKLHPTWKYTTCP
                     VCGGKAERDTDTMDTFMCSSWYHLGYLSPYYPTWPFDQKEYDYWMPVDVYTGGIEHAT
                     MHLIYTRFFHKACRDMGITKGNEPMLKLRNQGSVLGEDNEKMSKSRGNVVDPDKLVAT
                     YGADTVRAYLMFFARWELGAPWNSSGIEGSYRWLRRMWSMFLEPEEKFSEIDPEVLKK
                     LRRKTYQTLRSVTGDFEEFQFNTIVSSLMELLNEIAKAKQAGAWGTNEWNESVDVYLK
                     MAAPVVPHITEEIWHLLGHSDSIHLQKWPKMDEEAAKEEEITLVVQVNGKLRDRITLP
                     SDYSEEEAKELVFKRQAVQAAMGDKPVQKIIMVPKKLIN"
BASE COUNT          505 a          344 c          400 g          404 t
ORIGIN      
        1 ctgagcatcc tttggtcaac aaattaacca aaccggaaca agccgaaagt gtcaaagcat
       61 atatcgaaca agcaaaaaag caatcggata tacagcgaga atcagttgac agagaaaaaa
      121 ccggtgtttt taccggatca tatgcaatca atccggtaaa cggagccaga attccgattt
      181 gggttgccga ttatgtatta atgggctacg gaacgggcgc gattatggcg gttcccgctc
      241 atgatcaacg tgattttgaa tttgcgcgaa aatttggttt acaagtgatc gctgtcgttc
      301 aaccggatga tatggctccg ctggacggtg atacaatgaa agaagccgtc cccgctcatg
      361 gcagattaat caattctgga gagatgacag gcgtttccgg tgaggaagcg tttgaccgcg
      421 tcgtcgattg gctcgaaaaa aatcacttgg ggcataaaac cgttacttat cggttgcgtg
      481 attggctgat ttcccgtcag cgttattggg gagcacccat tccgatgatc cattgtccaa
      541 aatgcggcgt ggttccggta cccgaagatc aactccctgt tttattaccc gacgatgtcg
      601 agtggaaacc caccggagaa agcccattaa agcttcatcc tacatggaaa tataccacct
      661 gtcccgtttg cggcgggaaa gccgaacggg ataccgatac gatggatacc tttatgtgtt
      721 ccagttggta tcatttgggt tacctcagtc cgtattatcc aacatggcct tttgatcaga
      781 aagaatatga ttattggatg ccggttgacg tatacacagg cggaatcgag catgcaacca
      841 tgcacctgat ttatacacgc ttcttccata aggcctgccg tgatatgggc ataaccaaag
      901 ggaatgaacc aatgctcaag ctccgaaatc aagggagtgt tttaggtgaa gacaatgaaa
      961 aaatgtcaaa aagtcgcgga aatgtggttg atccggataa gctcgttgcc acttatggcg
     1021 cagatacggt tcgggcatat ttaatgtttt ttgcccgctg ggagcttggt gcgccatgga
     1081 acagttccgg aatcgaagga tcttatcgct ggcttcgaag aatgtggtcc atgttccttg
     1141 aaccggaaga aaaattttcc gaaatcgatc cggaagtttt gaagaaactt cgcagaaaaa
     1201 catatcaaac attgcgatcg gtaaccggag attttgaaga attccaattt aatacgattg
     1261 tttcaagttt aatggaacta ttaaacgaaa tcgccaaagc gaaacaagcc ggagcatggg
     1321 gaacaaatga atggaacgag tcggtagatg tctatttgaa aatggccgca ccggttgtcc
     1381 cccatatcac cgaagaaatc tggcacttac tcggacattc ggattcaatc catttacaga
     1441 aatggccaaa aatggatgag gaagctgcta aggaagagga aataaccctt gttgtacagg
     1501 tcaacggcaa attacgggat cggataactt taccttcaga ttactctgaa gaagaagcaa
     1561 aagagctcgt attcaaaagg caagcggttc aagcagccat gggagataaa ccggtacaaa
     1621 aaatcatcat ggttccaaaa aaattaatca ata
//
LOCUS       DAOMIV010000491         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_87808_length_1641_cov_4.967844, whole genome shotgun sequence.
ACCESSION   DAOMIV010000491 DAOMIV010000000
VERSION     DAOMIV010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87808_length_1641_cov_4.967844"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PKV59_09400"
     CDS             <1..391
                     /locus_tag="PKV59_09400"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQP47006.1"
                     /translation="ALKAIAHGATTILGEMVDYDLNKMTPEVVFNAALKGDEIAKNIY
                     ETAGYYLGIAIGNSIVQLEPERIVIGGGIAQAGEILFAPMRRAIQEHVHLVPLERIQI
                     VPAQLGNNAGVIGTSMWAEYMVRRKAL"
     gene            complement(598..1293)
                     /locus_tag="PKV59_09405"
     CDS             complement(598..1293)
                     /locus_tag="PKV59_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQP47007.1"
                     /translation="MKKEIPTKKHLEKLAEYNLQTIPPEKWMSVRYQPGEAITREGVE
                     IENLALVIGGMAKVYRTAPNGRNLILCYYLSDGVIGEIELLTRRKRATSTVTAISEFE
                     CIAIDSEICAAELKTNIPFSNKLGTTLAEKLERSTDNYVLSALCSAEERLCTYILQNA
                     PTGLFRDVLTDVACSVGTSYRHVLRLLSELCSEGILRKSESGYEMMNREELIRRSRAT
                     MNQKSGIVKKELR"
     gene            1404..>1641
                     /locus_tag="PKV59_09410"
     CDS             1404..>1641
                     /locus_tag="PKV59_09410"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQP47008.1"
                     /translation="MVQIAALLTGITLAVMISINGHLTAEFGIFPAAAIIHAVGSLFA
                     MILCAFQKPKKPLWGHHPLWIYLGGAIGVFTTVFN"
BASE COUNT          386 a          417 c          400 g          438 t
ORIGIN      
        1 cgctttgaaa gcgatcgcac acggcgctac gacgattttg ggagagatgg tcgattacga
       61 cttaaacaag atgacaccgg aagtggtctt caacgcagcg ttgaagggtg atgaaatcgc
      121 gaaaaacatc tatgaaacag ccggatatta cctcgggatc gcaatcggaa attccatcgt
      181 ccaactggag ccggaacgga tcgtaatcgg gggcggcatc gcgcaggccg gcgaaatcct
      241 gtttgcgccc atgcgcagag cgatccaaga acatgtccat ttggtcccac tcgaaaggat
      301 tcagattgtt cccgcccaat tggggaacaa cgccggagtg atcggcacat ccatgtgggc
      361 ggaatacatg gtccgccgca aagcgctctg agttttcctc aaaccgccgt tcgggtataa
      421 aaaatcccgg ctgttcgaat cacaacaacc gtcttcgatc gggttcattt tctttttccg
      481 ccttcggcga ggggattttt tgcgggtttc gatcatccga ttcgggggaa aaaacctttt
      541 cagacagaac ttgcgatatt tccggaattt tgaacggcac cttcgttcgg ctgccggtta
      601 gcggagttct ttcttcacaa ttccgctttt ttgattcatc gttgcccgag aacggcggat
      661 cagctcttcg cggttcatca tttcatatcc gctctcggat ttccgtaaga tcccttcgct
      721 acaaagctcc gacagaagac gaaggacatg acgataactt gtaccgacgg aacaggctac
      781 atcggtcaat acatcgcgaa acaagccggt gggagcgttc tgcagaatat aggtacaaag
      841 acgttcttcg gcagagcaca acgccgacaa cacataatta tcggtgcttc gttccaactt
      901 ttccgccaac gttgtcccaa gcttgttcga aaacgggata tttgttttca gttccgcggc
      961 acagatttca ctgtcaatcg cgatgcattc aaattcggag atagcggtaa cggtgcttgt
     1021 ggcacgtttc cgccgggtga gtaattcgat ttccccgatc actccgtcgg aaaggtaata
     1081 acaaagaatc agattcctgc cgttgggtgc cgtgcgataa actttcgcca ttccgccgat
     1141 gaccagtgcc aggttttcaa tttcaacacc ttcccgggtg atggcttcac ccggctgata
     1201 gcgtacactc atccactttt ccggcgggat cgtttgcagg ttatattccg cgagtttctc
     1261 cagatgtttt ttggtcggga tctctttttt catatctttt cgcctttctc tctattagat
     1321 cagaaaacgc aaaaaacacc atgacaaatg tcctactccc ttcgctgctt atttgatatg
     1381 ctttcgatta gggacacaga aaaatggttc agatagcggc tcttttaacc ggcattacgc
     1441 ttgcggtcat gatatccata aacgggcatt tgaccgcaga attcgggata tttccggcgg
     1501 cggcaattat ccatgcggtc gggtctctgt ttgccatgat cctttgcgct tttcagaaac
     1561 ccaaaaagcc gctctggggg catcatccgc tttggattta tttgggcgga gcgatcggtg
     1621 tctttaccac ggtgttcaat a
//
LOCUS       DAOMIV010000492         1636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_88259_length_1636_cov_3.768501, whole genome shotgun sequence.
ACCESSION   DAOMIV010000492 DAOMIV010000000
VERSION     DAOMIV010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88259_length_1636_cov_3.768501"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1629)
                     /gene="dnaE"
                     /locus_tag="PKV59_09415"
     CDS             complement(<1..1629)
                     /gene="dnaE"
                     /locus_tag="PKV59_09415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQP47009.1"
                     /translation="MTDSNRFRMSDKSYYLRSPEEMEKLFGYIPGAIENTLAIAERCN
                     VDLSSKGHHLPIFPLETGETAESELRKLCEIGLKERYGDRVDREPVIRERIEKELGII
                     HQMGFDAYFLIVQDLTRHAREENIWYNVRGSGAGSMVAYVLGITPVDPLKFKLIFERF
                     LNPERISMPDIDLDIQDDQRYKMLQYCSDKYGADRVSQIITFNTLGAKGAIRDVGRVM
                     DIPLSEVDRICKLIPAGIKMPVSGKSITLNNCLEEIPEFSDAVRADPRLTELVTTASE
                     MEGVTRNVGTHAAGVIITDVPIVEYAPLHRPTSGSDDNPIKSVAQFEMNYVDKMGLLK
                     IDFLGLATLTVMQKCCAMIEKRHGVHLDLQNIPTDDKKTFTYLSQGHTAGVFQLEGSG
                     MTRYIKEMKPKSLDNIIAMVALYRPGPMQFIPDYIACMHGEKEPFYRHERLKPIFAET
                     YGIPVYQEQIMSAVMELAGYTAAESDSFRKAISKKKKAEIEEHREKFVSGCVKNGIEK
                     TTAEEIFKDWEQFANYGFNKSHAADYGVLAVQTGY"
BASE COUNT          420 a          436 c          322 g          458 t
ORIGIN      
        1 ataccccgtt tgaacagcta aaactccata atcagccgca tgacttttgt tgaaaccgta
       61 attcgcaaat tgttcccaat ctttgaaaat ctcttcggcg gttgtttttt caatcccgtt
      121 tttaacgcag ccggaaacaa atttctctcg gtgctcttcg atttcagcct ttttcttctt
      181 ggaaatagct ttgcgaaaac tgtccgattc ggcagcggta tagcctgcca attccatcac
      241 cgcagacata atctgttctt gatataccgg aatcccgtac gtttccgcaa aaatcggttt
      301 caaccgttca tgccgataaa agggttcctt ttcaccgtgc atacaggcga tataatccgg
      361 aataaactgc atcggtcccg ggcgataaag cgctaccatc gcaatgatgt tatccagaga
      421 tttcggcttc atctctttga tataccgggt catacccgaa ccttccagtt ggaacactcc
      481 ggcagtatga ccctgactca agtaagtaaa agtcttttta tcgtcggtcg gaatattctg
      541 cagatctaga tgaaccccat gccgtttttc gatcatggcg cagcatttct gcattactgt
      601 gagcgtggct aaccctaaaa agtcgatttt cagcagcccc attttgtcga cataattcat
      661 ttcaaattga gctacggatt ttatcgggtt atcatccgat ccggatgtcg gtcgatgaag
      721 aggggcatat tcgacgatgg gtacatcggt aatgattaca cccgccgcat gtgtgccgac
      781 attccgcgtc acaccttcca tctcggaagc cgtcgttacc aactccgtaa gcctgggatc
      841 agcccgcacc gcatcgctga attcgggaat ttcttccaga caattattca acgtaatgga
      901 ttttccgctc accggcattt taattcccgc cggaatcagt ttgcagatac ggtctacttc
      961 cgacaagggg atatccatca cccgcccgac atcgcgtatg gcacctttcg cacccagtgt
     1021 gttgaaagta ataatctgtg aaacccgatc ggcaccgtac ttatccgaac aatattgcag
     1081 cattttatat cgttgatcat cctgaatatc cagatcgata tcgggcatcg aaattctttc
     1141 cggattcaaa aaccgttcga aaatcaactt aaatttcaac ggatcgaccg gtgtaattcc
     1201 caatacataa gctaccatcg aaccggctcc ggaaccgcga acgttatacc aaatattctc
     1261 ctctcgagca tgccgcgtca aatcctgaac aatcaaaaaa taggcatcga atcccatttg
     1321 atgaataatg ccgagttcct tttcaatccg ttcacgaatg accggttccc gatcgacacg
     1381 atcaccgtat ctttctttca agccgatttc acataatttc cgcaactcgg attcggcggt
     1441 ttcacccgtt tccaacggaa aaatcggcag atgatgccct ttgctgctca aatcgacatt
     1501 gcagcgttcc gcgatcgcca atgtgttttc aatcgcgccc gggatatacc cgaaaagttt
     1561 ctccatttct tccggagaac gcagataata ggacttatct gacattctga atcggttgct
     1621 gtccgtcaat ttcgca
//
LOCUS       DAOMIV010000493         1633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_88483_length_1633_cov_3.107098, whole genome shotgun sequence.
ACCESSION   DAOMIV010000493 DAOMIV010000000
VERSION     DAOMIV010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88483_length_1633_cov_3.107098"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1448)
                     /locus_tag="PKV59_09420"
     CDS             complement(123..1448)
                     /locus_tag="PKV59_09420"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HQP47010.1"
                     /translation="MAQTLTTCPRCQHQMTANIQQIFDLHEDPSDKEKLLTGQVNMAV
                     CPSCGYQSAIGVPLVYHDPEKELLLTYFPSELGLPINEQEKILGPLINKVVERLPLEE
                     RKGYLLRPQAMLTYDTLIEKILEADGITKEMIQNEEKKVNLLRQLLTCSEESIPEIIK
                     QEEAQIDQSFFTLFTNIQASAVQSADDATRKRIKAIQDVLLTETAYGRELKKKAESTQ
                     KAIKDLQDLGDKLNRDTLLELVLNSPDETYLQTLAGLARDGMDYQFFTNLSGRIEKAS
                     SDEDKKKYTEIREKLLEMVQQIDAAIAGQKQLRKQVFDEIMKEEDLEQGIARYARAID
                     DSFLEIAQSELAAARKNGDFIRSGKIQKLIDTIEKMSEMPPEVEFLEELLQKTDVAEL
                     TATLDANKEKVTDDFKKILDSLIAQLQQTPDQQKELLEKLKLIQKIINL"
BASE COUNT          404 a          365 c          334 g          530 t
ORIGIN      
        1 atgccatcat caccgatgat cccaaattag gattgattta caatctcaat cagtcctgat
       61 tcagtaaatt ttcatttaaa caaaacgggg aaggcatctt ccctgttttg ttttcaacga
      121 tatcacagat taattatttt ttgtatcaat tttagtttct ccagcagttc cttctgttga
      181 tctggagttt gctgcaactg cgcaattaat gaatccagaa ttttcttgaa atcgtcggtc
      241 actttttctt tattggcatc cagcgtcgca gtcaattccg ccacatcggt tttttgaagc
      301 aattcttcca aaaactcgac ttcgggaggc atttcgctca ttttttcgat cgtgtcgatt
      361 agtttctgaa tcttcccact tcgaataaaa tcgccgtttt tccgagcggc agccaattca
      421 ctttgcgcga tctccaagaa ggaatcatca attgcacggg catatcgggc gatcccctgc
      481 tcaagatctt cctctttcat gatttcgtcg aatacctgtt tgcgcagctg tttttgacca
      541 gcaatcgctg cgtcaatctg ttgaaccatc tccagtaatt tctctcgaat ttcggtatat
      601 ttcttcttgt cttcatcgga agaagctttt tcgatccgac cggataaatt ggtgaagaat
      661 tgataatcca tcccatcgcg ggccaaacct gccaatgttt gcagataggt ttcatccggc
      721 gagttaagca ccagttcgag cagcgtatcc cgatttagtt tatcgcccaa atcctgaaga
      781 tctttgatag ctttctgcgt gctttccgcc ttctttttca attctcgacc gtaggctgtc
      841 tccgtcaaca agacatcctg tatggctttg attcgtttac gggttgcatc atcggcggat
      901 tgaacggcgc ttgcttgaat atttgtaaaa agtgtaaaga aagattggtc aatctgcgcc
      961 tcttcctgtt tgatgatttc gggaatggat tcttcggagc aggttaataa ttgccttaat
     1021 aggttaactt ttttctcctc gttttgaatc atctctttgg taatgccgtc cgcttcgagg
     1081 attttttcga tcaacgtatc ataggtaagc atcgcttgcg gtcgcagcag atatcctttt
     1141 ctttcttcca acggtaagcg ctccactact ttgttaatca acgggcccaa aattttctct
     1201 tgttcgttga tcggcagccc gagttcggaa ggaaagtagg tcaaaagtaa ttctttttcg
     1261 ggatcatgat aaaccaaagg gacgccgatt gcactctgat agccgcaaga cgggcaaaca
     1321 gccatattta cctggcctgt cagaagtttt tccttgtccg agggatcttc atgaagatca
     1381 aaaatctgtt gaatattcgc cgtcatttga tgctgacagc gtgggcatgt tgtcaaagtt
     1441 tgagccataa tagctccctt cctgttttaa tatcctaaaa attataccaa attcggataa
     1501 acattttatt aacacggctg tgaatgaggg aaaaatctga caaaatggcg gaaggtttcc
     1561 gttttcaacg gatagattca aaatggagcc ggactttttg caagcctacc ggagagagat
     1621 atgagagggc ttc
//
LOCUS       DAOMIV010000494         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_88732_length_1630_cov_4.613968, whole genome shotgun sequence.
ACCESSION   DAOMIV010000494 DAOMIV010000000
VERSION     DAOMIV010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88732_length_1630_cov_4.613968"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PKV59_09425"
     CDS             complement(<1..769)
                     /locus_tag="PKV59_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HQP47011.1"
                     /translation="MSQPLSSFLLLVVLIIINGIFSMTEMAIVSVRKARLQHEMEKGN
                     RKARTALELYENPNDFFSTIQIFITLVGVITGAVGASAFSGPVANLLAKVPLFSSIAN
                     TLALLLVSIVITYFSLVIGELIPKRLAISFPEKISLNMSGIMRVLSRITKPLVSFLSW
                     STEIGIKILGVKKTEDDSVSEDEVKVMIEQGTLSGVFDETEQDIVESVFRMSDKTVDG
                     MMTPRTELCWIDLDEPLEESLAEIKESDDSYFPLVRGT"
     gene            complement(813..1496)
                     /locus_tag="PKV59_09430"
     CDS             complement(813..1496)
                     /locus_tag="PKV59_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47012.1"
                     /translation="MAHYSITGALFSEFQMAALGQEVLLPEGVMKDRRISYTALGHIH
                     KFQDLNAGNQPPVVYPGSIERVNFGECNDKKGFVIAQIDNRHSSYTFRELEGRNFFSN
                     EITIQNAETFQQEALAALPEADQIPDAMIRLIIHYPREWENFLDERELRKAAESALAF
                     HLVLRPISSPRIRLSEDAAVSSLSHLELLERYCESNKYDKNALPGLLNLAKQIFNDVD
                     MGISEGSET"
BASE COUNT          465 a          385 c          334 g          446 t
ORIGIN      
        1 ttgttccccg aaccaaagga aaataactgt catccgattc cttaatttcc gccaagcttt
       61 cttccaacgg ttcgtccaaa tcgatccagc ataattcggt tcggggagtc atcatcccgt
      121 caaccgtttt atcactcatt cggaatacac tctcgacaat atcctgttcg gtttcatcaa
      181 aaacaccgga gagagttccc tgctcgatca ttactttgac ttcatcttcc gaaacagaat
      241 cgtcttcggt tttcttgaca cccaaaattt tgataccgat ttcggtcgac caacttaaga
      301 agctgaccag cggcttagtg attcgggaca gcacacgcat tatcccgctc atgttcagag
      361 agatcttttc ggggaaagaa attgcgagcc gtttcggaat caattcaccg attaccaaac
      421 tgaaataagt gatcacgatc gaaacaagca aaagcgctaa ggtatttgct atggaggaga
      481 aaaggggaac ctttgccagt aaattggcta cgggaccgga aaaggcggag gcgccgaccg
      541 caccggtgat aacaccgacc aatgtgatga aaatctgaat ggttgagaaa aagtcattcg
      601 gattttcata caactccaag gctgtccttg cttttcggtt tcctttctcc atttcatgct
      661 ggaggcgggc ttttcgaacc gaaacaatcg ccatttccgt cattgaaaaa attccgttaa
      721 taataatcag aacaactaat aataaaaaac tgctaagtgg ttgtgacata acctttcaaa
      781 tccatcattt ttaaaaaacc ggtaccctat tttcaggttt cagaaccttc agaaatcccc
      841 atatccacat cattaaatat ttgctttgca aggttcagca atccgggaag cgcatttttg
      901 tcatatttat ttgattcaca ataacgttcc agcagctcta aatggctcag gctgcttaca
      961 gcggcatctt ccgaaaggcg gatccgcgga gatgaaatag gcctcaatac caaatgaaaa
     1021 gccagtgcgc tttccgcggc ttttcgtaat tctctttcat ccaaaaaatt ctcccattcg
     1081 cgcgggtaat gaatgattaa acggatcatc gcatccggaa tttgatcagc ctccggcaaa
     1141 gcagccaaag cttcctgttg aaaagtctcg gcattttgaa tagtgatctc gttggagaaa
     1201 aaattcctgc cttctaactc acgaaaagta taagaggaat gccggttatc gatttgagcg
     1261 atgacaaacc cttttttatc attgcattcc ccgaaattaa ctctttcgat tgaaccgggg
     1321 taaaccacag ggggttgatt cccggcattc aagtcttgaa atttatgaat atgtcctaaa
     1381 gcagtatagg atatccgccg atctttcatc actccctcgg ggagtagcac ctcctgtccc
     1441 aaagcagcca tttgaaattc ggagaacaga gcaccggtta tggaataatg tgccatcaga
     1501 ataatcggga gggatggatc cgtttcatca agaatacgat tgatttccgt cgatatttga
     1561 tatgcgagat cctgtccggt actttcggtt tctttgccaa ttgactaggt tgccgccacc
     1621 agacgggagc
//
LOCUS       DAOMIV010000495         1630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_88771_length_1630_cov_2.727619, whole genome shotgun sequence.
ACCESSION   DAOMIV010000495 DAOMIV010000000
VERSION     DAOMIV010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88771_length_1630_cov_2.727619"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1381)
                     /locus_tag="PKV59_09435"
     CDS             complement(<1..1381)
                     /locus_tag="PKV59_09435"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQP47013.1"
                     /translation="MEVQLADGRIIPVESHRIKIVQPTKFLPIDERLTAIRNAGFNTF
                     LLQNHDIFLDMLTDSGTNAMSDTQQSAMLRSDDAYAGSESFIRLQEAVKDIFRMDYVI
                     PAHQGRACEHLLTKVFVKPGDVVITNYHFTTTKAHIVNNGGQVIELIYKEGRNTNSDN
                     PFKGNMDLEALERVIAEKGADKISFVRMEATTNLIGGQPFSMENLKAVSEIAHKNHLL
                     LVIDCSLISENAFFIKTREAGFSEKSIPEIIQEMLQQADLIYMSGRKSTMARGGMICT
                     NDEKIYHEILPLVPVFEGFITYGGMSTKEIEAMAVGLREMVDFNVAGSGAEMINFFTN
                     RLINLGVPVVTPPGGLGCHVDAIKFLPHLSQTEYPAGALAAAFYIVSGCRGMERGTLS
                     MDRDKNGKEEVSDMELVRLAVPRRSFTMSQIEFAADRLAWLYQHRDLVHGLKWIEEPP
                     VLRFFLGKLT"
BASE COUNT          457 a          355 c          307 g          511 t
ORIGIN      
        1 aagttaattt tcccaaaaag aaacgtaata caggcggctc ttcaatccat ttcaacccat
       61 gaacgagatc tctatgttga taaagccatg ccaagcgatc agcggcaaat tcaatttggg
      121 acattgtaaa agatcgtcgc gggactgcca gacgcacaag ttccatatcc gatacttctt
      181 ctttaccgtt tttatctcga tccatagata atgttccccg ttccatccca cgacatccgg
      241 agacaatata aaaagcggct gctaaagcac cggcgggata ttcggtttgt gaaaggtgcg
      301 gaaggaattt tattgcgtcg acatgacatc ccaagccgcc tggaggagtc accacgggga
      361 cgcctaaatt gattaagcgg tttgtgaaaa agttaatcat ttccgccccg cttccggcaa
      421 cgttaaagtc aaccatttct cttaacccca ctgccattgc ttcgatttct ttggtagaca
      481 taccaccgta cgtgataaag ccttcgaaca cgggtaccaa tggcaatatt tcatgataga
      541 ttttttcgtc gtttgtgcaa atcatacctc cgcgcgccat tgtgcttttt cttccggaca
      601 tataaattaa atcggcttgt tgtaacattt cttgtataat ttccggaatg gatttttcgg
      661 aaaaccctgc ttctcgagtc tttataaaga aggcattttc agaaatcaga ctgcaatcta
      721 ttacaagaag aagatgattt ttatgagcaa tttcgctgac agcctttaaa ttttccattg
      781 aaaaaggttg tccgccgatt aggttcgtgg ttgcctccat tcgaacaaaa gaaattttgt
      841 ctgctccttt ttccgctatc accctttcta aagcttcaag atccatattt cctttaaaag
      901 ggttatcact gtttgtgttt cttccttcct tataaattaa ttcaatgact tggcctccgt
      961 tatttacgat atgggctttt gtcgttgtga aatgataatt cgtaataacg acatctcccg
     1021 gctttacaaa cacctttgtc agtaaatgtt cacatgctcg cccctgatga gccgggataa
     1081 cataatccat ccgaaatata tctttcaccg cttcctgtag gcgaataaaa ctctctgatc
     1141 cggcgtaggc atcatctgag cgtaacattg cactttgttg ggtatcactc attgcattcg
     1201 tgccactgtc ggttaacata tccagaaaaa tatcatgatt ctgcagaaga aaagtgttaa
     1261 aacctgcatt ccgaatagct gttaaacgtt catcgatcgg taaaaatttg gttggttgga
     1321 caatcttaat tcgatgcgac tcaaccggaa taatccgacc atctgctaat tgaacttcca
     1381 tattgatcct ccgttgcttt tcgcttgtaa taaataaaaa aaaaagcgat cccggccggg
     1441 atcgcttttt aattgactat ggttaattct tacgcaaaaa ctccggcggg tttaaatcgg
     1501 ctgccgtaat aataatatgc gtagaaatta ctgaataatt gcatgtgttc tattgtaaag
     1561 tcgagaatgg agttatgtca aggagtttta taattttatg atccgaatac catttcctat
     1621 ttttatggat
//
LOCUS       DAOMIV010000496         1627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_89013_length_1627_cov_3.714377, whole genome shotgun sequence.
ACCESSION   DAOMIV010000496 DAOMIV010000000
VERSION     DAOMIV010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89013_length_1627_cov_3.714377"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="gcvPB"
                     /locus_tag="PKV59_09440"
     CDS             <1..931
                     /gene="gcvPB"
                     /locus_tag="PKV59_09440"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQP47014.1"
                     /translation="VMSGFECLAVRTDERGNIDLNDLTSKCNEQLAGIMITNPNTLGL
                     FDENIVEVIRIVHQAGGLVYGDGANMNALLGIVKPAELGFDVMHFNLHKTFATPHGGG
                     GPGAGAICCTEQLKAYLPGPIADILEEATEEVPPLYGYRMPEKSIGMLRSFWGHFGII
                     VRAYCYCMMLGAEGFRQVAEYATLNANYLMEALKQDYPLPFDRTCMHEFVLDVKWPDA
                     PGIRALDVAKRLMDYKFHPPTNYFPLIVHEALMIEPTETESKETLDDFIKAMKAIAKE
                     ARENPELLHRAPAETPFGRMDEVKAARDLKIAE"
     gene            1087..>1627
                     /locus_tag="PKV59_09445"
     CDS             1087..>1627
                     /locus_tag="PKV59_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HQP47015.1"
                     /translation="MRDIIDTKTDIEIKTSPKIPAGAIRLQIVSGAKSELLKEKGDSV
                     VEKEKTKTTVSFYTGEKINADTYRSLGGIIAKWIKKNGIKTAALQIPADLNDKDHKAL
                     LEGLLLGSFSFDKYKKKEDPVDSVIYLDPSEEILLQTCKTVCDAVNMAREWAHEPAAI
                     INPQTLSERAQKFAKQFGLN"
BASE COUNT          487 a          358 c          399 g          383 t
ORIGIN      
        1 cgtcatgagc ggctttgaat gccttgcggt ccgtaccgat gaacgcggca atatcgattt
       61 aaatgatctc acatccaaat gcaacgagca gctggccgga atcatgatta ccaatcccaa
      121 taccctcgga ttgtttgatg aaaatattgt cgaagtgatc cgtatcgttc atcaagccgg
      181 cggactggtt tacggtgacg gcgccaacat gaatgccttg ttggggatcg ttaaaccggc
      241 cgaactggga ttcgacgtca tgcattttaa tctgcataaa acttttgcca ccccgcacgg
      301 cggcggcgga ccgggcgccg gtgccatctg ctgtacggag cagttgaagg catatctgcc
      361 gggtccgatc gcggatattt tggaagaagc gaccgaagaa gtacccccgc tttacggata
      421 ccgtatgccc gaaaaaagta tcggaatgct gcgatctttc tggggtcatt tcggaattat
      481 tgtccgggct tattgctatt gtatgatgtt gggggcggaa ggttttcgac aggtcgccga
      541 atatgctact ttaaacgcca attacctgat ggaagcattg aaacaggatt acccgttgcc
      601 gtttgatcgc acctgcatgc atgaatttgt tctggatgta aaatggccgg atgcacccgg
      661 aatacgggca ttggatgttg ccaaacgtct gatggactac aaatttcatc ctccgacaaa
      721 ctatttccca ttaatcgttc acgaagcttt gatgatcgaa ccgacggaaa ccgaaagcaa
      781 agagacgctg gatgatttca taaaagccat gaaagcgatt gccaaagagg cacgtgaaaa
      841 tcccgaatta ttgcatcggg ctccggcgga aacgcccttc ggacgaatgg acgaagtgaa
      901 agcagccaga gatttgaaaa tagccgagta atggaataaa aacaaaccgt tgtcattccg
      961 cgggcctttt cgaaagaagg aggggatttt ttttgaaacc ggcaaaaacc ttacccatcc
     1021 gattgaaagg ttccccgcgg aatgacgatt tgtaatcaat taaccgatat gatataaaga
     1081 ggaaaaatga gagacataat tgacacgaaa accgatattg agattaaaac atccccaaaa
     1141 attcccgccg gagcaatccg gctgcagatc gtcagcggag caaaaagtga attattaaag
     1201 gaaaaagggg attcggttgt cgaaaaggaa aaaacaaaaa caaccgtttc tttctacacc
     1261 ggtgaaaaaa tcaatgccga tacataccgc tccctcggag gaatcatcgc gaaatggatc
     1321 aagaagaacg gcatcaaaac cgcggcgctt caaatccccg ccgatctgaa cgacaaagat
     1381 cataaagctt tgttggaagg gttgttgttg ggttcgtttt cctttgacaa gtacaagaaa
     1441 aaagaggatc ccgtcgattc cgtcatttat cttgatcctt cggaagaaat attgttgcaa
     1501 acatgtaaga cggtttgtga tgcggtgaat atggcacggg aatgggcaca tgaaccggct
     1561 gcaatcatta atcctcagac actttccgaa cgggcacaaa aatttgcaaa acaattcggc
     1621 ttaaatt
//
LOCUS       DAOMIV010000497         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_89667_length_1619_cov_4.903453, whole genome shotgun sequence.
ACCESSION   DAOMIV010000497 DAOMIV010000000
VERSION     DAOMIV010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89667_length_1619_cov_4.903453"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PKV59_09450"
     CDS             complement(<1..487)
                     /locus_tag="PKV59_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQP47016.1"
                     /translation="MTFEIEYAILSEYLSDLFFRSLTVEGDCKIMQRERISENVYWFQ
                     SEIYAQVTAGVIIGPQWAVVVDTLALPEEAQEIRKFVTETIGVPIRYLVNTHFHADHA
                     WGNCFFPGATIIAHTLCAKYLKEIGIPALEETKKQNPSFLNSHIVLPHLTFSHGELIL
                     KI"
     gene            complement(647..719)
                     /locus_tag="PKV59_09455"
     tRNA            complement(647..719)
                     /locus_tag="PKV59_09455"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(683..685),aa:Glu,seq:ttc)
     gene            complement(895..1572)
                     /gene="rsmI"
                     /locus_tag="PKV59_09460"
     CDS             complement(895..1572)
                     /gene="rsmI"
                     /locus_tag="PKV59_09460"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQP47017.1"
                     /translation="MRKNNALYVVATPIGNDDDITLRAINTLKSADALICEEYRNGNR
                     LLRKLNIENKEIISLNEHNEPAQIENILIRLAQGQSLALVSDCGTPLFSDPGHKLISE
                     VSAAGFRVVPVPGASALTAAISVAPLHLKKFYFAGFLPPASEGRLAELRKIKSRQENI
                     PVILMDTPYRLAAVLKDVCNIFGKEHKITLACDLTTEDERILTGSCAEIMKEIGAKKA
                     EFMLIIH"
BASE COUNT          472 a          326 c          324 g          497 t
ORIGIN      
        1 cgattttcaa gattaattca ccgtgagaga aggtcaaatg agggagaacg atatgagaat
       61 tcagaaaaga cgggttctgt ttttttgttt cttccaaagc cggaattccg atttctttta
      121 agtacttggc acacagcgta tgtgcgataa tcgtcgctcc cgggaaaaaa caatttcccc
      181 aagcgtgatc cgcgtgaaaa tgagtattga ccaaatatct tatcggaact ccgatagtct
      241 cggtgacaaa tttcctgatt tcctgtgctt cttccggaag tgccagagtg tcgaccacaa
      301 cagcccattg aggtcctata atgacacctg cggtaacctg ggcatagatt tcactttgga
      361 accaataaac attttcagaa atacgttcac gctgcataat tttacagtcc ccttcgacag
      421 tcaatgagcg gaaaaataaa tcgctcagat attctgatag aatagcatat tcaatttcaa
      481 aagtcaaaat atcactgatt tttgtcttga aaatgctata ttccctcaat attaatggat
      541 attgagtgta tcatttttcc gcagggaaat attaaatttt gatagaaata tgatcattat
      601 tatccgtacg gataaaaaaa gatatccgac tcggatatct tttcagcacc tccagtgaga
      661 ttcgaactca cgttttaacc ttgaaagggt tgcgtcctgg tcccctagac gatggaggct
      721 tgttgaataa cgttgctatt ttagcagttg tttttctatc cgtcaagata agtctgaaac
      781 ttgtgttgtt attctccaaa ttgtcatcct gaaaaaaggg taagaaaaat gagtttttga
      841 tagaattcga tcaaatttgc ggattttcga acaaagacct atttttcgac ttctttaatg
      901 aattatcagc ataaattcgg ctttctttgc cccgatttct ttcatgatct ctgcacacga
      961 tccggttaaa atccgctcat cttcggtggt taaatcacaa gcaagcgtaa ttttatgttc
     1021 tttcccaaag atattacaaa catcttttaa tacagctgcc aagcgataag gcgtatccat
     1081 caaaatgacc ggaatatttt cttgtctcga tttaattttg cgaagttcgg caagtcttcc
     1141 ttcggaagcg ggcggaagaa atccggcaaa atagaatttc ttcaaatgaa gcggcgcaac
     1201 ggaaatagct gctgtcagag cagatgctcc cggaacggga accacacgaa aaccggctgc
     1261 ggaaacctcg gaaatcaatt tatgcccggg atcggaaaat aaaggcgttc cgcaatcgga
     1321 aacaagtgca aggctttgtc cctgcgctaa acgaatgaga atattttcga tttgtgccgg
     1381 ttcgttatgc tcatttaatg aaatgatttc tttgttttca atgttcagct ttcgcaacaa
     1441 ccggtttccg tttcgatatt cttcgcatat caatgcatcg gccgatttga gagtgttaat
     1501 tgctcgcaaa gtgatgtcat catcattccc gatgggcgtt gccacaacat aaagggcgtt
     1561 attttttctc atagctgaat tctatcagga tttttttggg agaaattatg atccggaac
//
LOCUS       DAOMIV010000498         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_89676_length_1619_cov_3.842072, whole genome shotgun sequence.
ACCESSION   DAOMIV010000498 DAOMIV010000000
VERSION     DAOMIV010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89676_length_1619_cov_3.842072"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PKV59_09465"
     CDS             <1..1120
                     /locus_tag="PKV59_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47018.1"
                     /translation="LKQLNDRVILAAAEKKDFYFLTALLDRKVSDTKVQLFRRSNQDF
                     KTRLLQCWNDAYYELNEVISDIRHALSDGRLLKTVFTKSKAFQYLKENIPGPHGFDQL
                     LVCLKVMRDKYNMDATVKISDLLSGATYEKVLKQYLINIPGVKHKIANWALTNITGHW
                     FVIDEPNIKPMIKKDLADKIPSHLAIESANADAIFEYWFGKLDEMKREYDRFSQEKFV
                     DAFPDFPPTACEYLPFIATQYLWFYGKKINMAKSRPKAIWAVAKEYEFVVPVIPKSNI
                     NGSIFNPCNIDWQKIQSIEDLITALPADIKPKTLWKIVPERLKEEIVTKARYLSEARS
                     FAAPAEWDGSLKQALLLMANERLPNGFKYWILKITAGH"
     gene            1186..>1619
                     /locus_tag="PKV59_09470"
     CDS             1186..>1619
                     /locus_tag="PKV59_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47019.1"
                     /translation="MRRLTIQQEQIRIHTFSGIAFDLLNPQPEMILIEDIAHSLALIN
                     RFNGAAIFPYSVAQHSLYVAGLLPPKLKLHGLLHDAAEAYVGDMVSPLKKIMPEYKEV
                     EAKISKVVADVFGLSYPAPAEVKKADLAVLSAEREQVLNPSYG"
BASE COUNT          509 a          314 c          351 g          445 t
ORIGIN      
        1 attaaagcaa cttaatgacc gggtcattct tgctgcagcg gagaaaaaag atttctattt
       61 tttgacagcg ttgctggata gaaaagtatc agatacaaaa gttcaattgt ttcgtcgctc
      121 gaaccaggat tttaaaactc gtcttctgca gtgctggaac gatgcttact atgaacttaa
      181 cgaggttatt agtgatatca ggcatgcact ttccgacggc cgacttttaa aaacggtatt
      241 cacgaagagc aaagcatttc aatatctaaa agaaaatatt cctggcccac atggcttcga
      301 tcaacttttg gtttgtctaa aagttatgcg agacaaatac aatatggatg caactgtcaa
      361 gataagcgat ttgctcagcg gagccacata tgagaaagtg ttgaagcaat atttaataaa
      421 tatccctggc gtaaagcaca aaatcgccaa ctgggcattg actaatataa caggtcactg
      481 gtttgtgata gatgaaccaa acatcaaacc catgatcaag aaggacctgg cggataaaat
      541 accatcacat ctggcaattg agtcagctaa tgccgatgca atatttgagt attggttcgg
      601 taaattagat gaaatgaaga gagaatatga cagattttca caggagaaat ttgtggatgc
      661 tttccctgat tttccgccga cggcttgtga atatcttcct tttattgcga cccagtatct
      721 ttggttttat ggaaaaaaaa taaatatggc aaaatcaaga cccaaagcaa tctgggctgt
      781 ggccaaagaa tatgaattcg tggttcccgt cattccaaaa tcaaatatta acggatcgat
      841 atttaatcct tgtaatattg attggcaaaa aatccaatcc attgaagatt taattactgc
      901 tcttccggcg gatatcaaac cgaaaacgct atggaaaatt gttcctgaac gactgaaaga
      961 ggagattgtt acaaaggcac gctatttatc agaggcaaga agttttgctg cacccgcaga
     1021 atgggatggc agtctcaaac aggctctttt attaatggca aacgagagat taccgaatgg
     1081 atttaaatat tggattctaa aaataacggc cggtcattga cgagaaaggg tgcctataag
     1141 atcgataaag gaatgaagaa ttaatttggt tttgagaata aagacatgag acgcttaact
     1201 atccagcaag agcaaattcg tattcacaca ttttccggca ttgcttttga tttgctgaac
     1261 ccgcaacccg aaatgattct tatagaagac atagctcatt cgcttgccct cattaatcgt
     1321 ttcaatggcg ctgcaatttt tccatatagc gttgcgcaac actcgctata cgtcgccggc
     1381 ctgttgcctc ccaaactcaa gcttcacggg cttttgcatg atgcagcaga ggcgtatgtc
     1441 ggtgatatgg ttagtcccct taagaaaatc atgcccgaat acaaagaagt cgaagcaaaa
     1501 atatccaagg ttgtggcgga tgtatttggt ttgtcgtatc cggcgccggc ggaggtaaaa
     1561 aaggctgacc tggccgtttt gtccgccgaa cgggagcagg ttctgaatcc ttcctatgg
//
LOCUS       DAOMIV010000499         1617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_89868_length_1617_cov_4.447503, whole genome shotgun sequence.
ACCESSION   DAOMIV010000499 DAOMIV010000000
VERSION     DAOMIV010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89868_length_1617_cov_4.447503"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /gene="lexA"
                     /locus_tag="PKV59_09475"
     CDS             <1..658
                     /gene="lexA"
                     /locus_tag="PKV59_09475"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQP47020.1"
                     /translation="KIMLFLEEYQKNNQFSPSIREIGNAIGVNSTSLVDYYLRQLAQM
                     GYLERNDRVSRSIRILKPFTEESQTAKNMISYSRQEVLRYPILGRIVASEPIPVPGSD
                     LAYFDPESYIEIPKKMIPASEKETELFVLEVSGDSMVDAMINDGDKVVFRQANQASNG
                     DMVAVWLSNNDETTLKYFYREGSRVRLQPANPTMGPIYIDDPEQVHIMGKVVMVIRHL
                     "
     gene            704..>1617
                     /locus_tag="PKV59_09480"
     CDS             704..>1617
                     /locus_tag="PKV59_09480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP47021.1"
                     /translation="MNNQAQQKKIAIISSLYMPHLGGVEKYSQSLAQTLTADYEVHVF
                     CMNTESVPAESRDGDIFIHALPCGGFFKGRLPVPTPKAIRRTKQIFRETGFDFGIIQT
                     RLYPFNLQAAKILKEFQIPFFLIEHGSAYVTFGNSLINRLWEYYERHQANILKRYCSD
                     FYAVSQASLDWLNHFGIQGKGIIHNSIDPQEFEGIPSGWRKSVGIPSEAILLTFAGRI
                     IQKKGIVTLLTAAERIEEERLHIIIAGDGDETLLSPWKQNPRIHFLGKVPHRVLLEIL
                     KDSQLFCLPTTYPEGLPTVILEAGYFGVP"
BASE COUNT          463 a          364 c          348 g          442 t
ORIGIN      
        1 aaaaattatg ctattcctgg aagaatatca aaagaataat caattttcgc catccattcg
       61 ggaaatcgga aatgcgatcg gcgtaaattc tacctcctta gtagattatt atttacggca
      121 attagctcaa atgggttatc tggaacgcaa tgatcgggtc tctcgcagca ttcgaatttt
      181 gaaaccattt acagaagaat cgcagaccgc aaaaaacatg atttcatatt cccggcagga
      241 agtactccgc tacccgatct tgggaagaat cgttgcgagc gagccgattc cggtgcctgg
      301 ttccgatttg gcatatttcg atccggaaag ttatattgaa atcccgaaga aaatgattcc
      361 cgcaagtgaa aaagagacgg agctcttcgt cttggaagtc tccggtgatt caatggttga
      421 cgccatgatc aacgatggag acaaagttgt ttttcgtcag gcaaatcagg cttcgaatgg
      481 cgatatggtg gctgtctggc tatccaacaa tgatgaaacc acactgaaat atttttatcg
      541 ggaaggatca agggttcgcc tccaaccggc aaatcccaca atgggaccga tttatattga
      601 tgatccggaa caagtccata ttatgggaaa ggtcgtgatg gttattcgtc atctataaac
      661 cgcttcaacc gatcggttta gattcttcag gtacaatcgc aaattgaaca atcaagctca
      721 gcaaaagaaa attgccatca tctcatcttt atacatgcct catctcggag gcgttgaaaa
      781 atattcccag tctctggctc aaacactgac agcggattat gaagtccatg ttttttgtat
      841 gaatacggaa tctgttcccg cggaaagtcg tgacggggat attttcattc atgcattgcc
      901 ttgcggcggt tttttcaaag gccggttacc cgttcccacc ccaaaagcca tccgccggac
      961 caaacaaatt tttcgagaga ccggatttga cttcggaatc atccaaactc gcctttatcc
     1021 tttcaattta caggcggcaa aaatattgaa agaattccaa attccttttt tccttatcga
     1081 acacggctca gcttatgtaa ctttcggtaa ctctctgatc aatcgcttat gggaatatta
     1141 cgaacgccat caagcaaata tattgaaacg ttactgctcg gatttttatg cggtatctca
     1201 ggcgagtctc gattggttga atcatttcgg aatccaaggg aaggggatca tccacaacag
     1261 catcgatccg caggaattcg aaggaattcc ttccggttgg cgcaaatcgg tcggaatccc
     1321 ttcggaagcg attctcctta cgtttgccgg gcgaatcatt caaaaaaaag gaattgtcac
     1381 tttattaacc gcagccgagc ggattgaaga agagaggctt cacatcatta tcgccggaga
     1441 cggagatgaa acgcttttaa gcccttggaa acaaaatcct cgaatccatt ttctcggcaa
     1501 agttcctcat cgtgtattgc tggaaatact caaagacagt caacttttct gtttaccgac
     1561 aacatatccg gaagggctgc cgaccgttat tttagaagcc ggttatttcg gtgtccc
//
LOCUS       DAOMIV010000500         1601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_91315_length_1601_cov_4.602846, whole genome shotgun sequence.
ACCESSION   DAOMIV010000500 DAOMIV010000000
VERSION     DAOMIV010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91315_length_1601_cov_4.602846"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKV59_09485"
     CDS             <1..194
                     /locus_tag="PKV59_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47022.1"
                     /translation="SKEDIFDIAERIERDSVLFTQEMIDLFPDLDPADFKVVLQEEKK
                     HLAMVLDKRMDSQLKSLRL"
     gene            372..866
                     /locus_tag="PKV59_09490"
     CDS             372..866
                     /locus_tag="PKV59_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQP47023.1"
                     /translation="MTKKWDGAEFFRLMAANEQAVGDWYRTLANDAKFGGKFFEKMAK
                     DEDRHYQIYTALLKKYEGTQALTVEVSEDHEAYLNALMKDHIESDADNLKAKAAKAST
                     KDDIYAIAEQVERDSVLFVEELIELYPDLKEEDFKVVLQEEKNHLAMVLTRRMDSQLN
                     TLRL"
     gene            complement(942..1376)
                     /locus_tag="PKV59_09495"
     CDS             complement(942..1376)
                     /locus_tag="PKV59_09495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ohr family peroxiredoxin"
                     /protein_id="HQP47024.1"
                     /translation="MGTITKYEAAVTNTGGRDGIVFSPDHSFSMGIEPPDKSGEKHTG
                     ASNPEQLFAAGYSACFNNALQSSLRRDKVEFESSTVTVFVALLYDPDEKGHRIGARIE
                     ASVKGISLDEAKKYVAIAHTRCPYSKAIQGNVDVKIDVVEAE"
BASE COUNT          435 a          307 c          404 g          455 t
ORIGIN      
        1 agagtaagga agatatcttt gatattgccg aacgaatcga aagagattcg gtgctgttta
       61 ctcaggaaat gatcgatctg ttccctgatt tggatccagc tgatttcaaa gttgttctgc
      121 aggaagagaa aaaacatctg gcgatggtgt tggataaaag aatggatagc caattgaaga
      181 gcctgcgatt ataaatttgt ttgagtttgc ttggtaagct gcctgttcat tccgtttggg
      241 ttgatcaggc ggctttttgt tttatgaaaa gtcggtaaat cttgactatt taacggttga
      301 ccgtaataat aaaaaaaggt ttgttttttt cgctaaaaaa tcgcaaagga aatatcgatt
      361 ggaggagtaa tatgactaaa aaatgggatg gtgcggagtt tttccgtctg atggcagcca
      421 atgaacaagc tgtgggagac tggtatcgaa cattggcgaa tgatgcaaaa ttcggcggga
      481 aattctttga aaaaatggcg aaggatgaag atcgtcacta tcagatctat acggctcttt
      541 tgaaaaagta tgaaggcact caggcattga cagttgaagt gtcggaagac catgaggctt
      601 atctgaatgc gctgatgaaa gaccatatcg aaagcgatgc cgataatctg aaagcaaagg
      661 ctgccaaagc ctccacgaaa gatgatatct acgcgattgc ggaacaagtc gaacgcgatt
      721 cggtactgtt cgtcgaggaa ttgattgagc tttatccgga tttgaaagaa gaagatttta
      781 aggttgtgct tcaggaagag aaaaaccact tggcaatggt ccttactcgc cgaatggata
      841 gtcagttgaa tacgctcaga ttgtaatatc gtatcgaaaa aattcgaaaa acaggctgta
      901 cccgaattac gatccggaac aacctgtttt tcgattaatt attattccgc ttcgactaca
      961 tcgattttta catctacatt tccctgaatc gctttggaat atgggcatct ggtatgagcg
     1021 atggccacgt atttcttagc ttcatccagg ctgattcctt tgacggatgc ctcaatgcga
     1081 gctccgattc ggtgtccctt ttcatcggga tcatataaca gcgcgacaaa taccgtgacg
     1141 gtactggact caaactccac tttatcccgg cgtaaagatg attgcagcgc attattaaag
     1201 caggcgctgt aaccggctgc gaacaactgt tccgggttgc ttgctccggt gtgtttttcg
     1261 ccggatttat ccggcggttc gatacccatt gaaaaagaat ggtccgggga gaaaacgatc
     1321 ccgtcccgac ctccggtatt cgtaactgct gcttcatatt ttgtaattgt gcccatcgtt
     1381 ttttcctttc ggttcgcggt ttagcgcttt tcttaaattt atcattattt cgggaaacta
     1441 ccctcgcgga tgttcgaccg tttgacatga tacgtatggg cgcatttccc ctcgtcctgt
     1501 gggagaggat tgtaggggac ggcgaaacca aaaaccctcg cccattggga gagggtaggg
     1561 tgagggaaaa atggagttgt gcaggctgtg tgtcgggtgt c
//
LOCUS       DAOMIV010000501         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_91564_length_1598_cov_4.298121, whole genome shotgun sequence.
ACCESSION   DAOMIV010000501 DAOMIV010000000
VERSION     DAOMIV010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91564_length_1598_cov_4.298121"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PKV59_09500"
     CDS             complement(<1..184)
                     /locus_tag="PKV59_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47025.1"
                     /translation="MKLVVLDGYTLNPGDIGWDKLEKFGDLTVYDRTNTGEVSDRIRD
                     ADIVFTNKTYVSRESIY"
     gene            complement(236..1003)
                     /locus_tag="PKV59_09505"
     CDS             complement(236..1003)
                     /locus_tag="PKV59_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HQP47026.1"
                     /translation="MFVEERQREILEFLHQNGKVLVKDLSERYQVTPDLIRKDLAKLE
                     KEGLLKRTYGGAIPLIQNSVPQPADDSHENNIEVKQRIAEKAETLIRNGDTVFLDLSS
                     INVEIARQLVRKNRNVTIVTNMINVLEEIPGESRVKLIFIGGKMNKLREGFTGAATIE
                     WMSRFQFDLSFIGVVGIDLVRNSATTYEIDDALTKTGAIKISNKSYIVAESSKFNSTG
                     NCRSASLNSFSGIITDAGLDPEIVEKLTQIGLQVLLV"
     gene            1169..1453
                     /locus_tag="PKV59_09510"
     CDS             1169..1453
                     /locus_tag="PKV59_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47027.1"
                     /translation="MIRYTRPLEDGNNYFIPEDRIVRIPAGYTGEPINRLAAYENLQQ
                     DLLNDLEKISQEMAGLREEGKIKSVHFKELMSQKMIDTRIISLFQSYGLE"
BASE COUNT          469 a          346 c          313 g          470 t
ORIGIN      
        1 catagattga ctcgcgcgac acataggttt tgttggtaaa aacaatgtcg gcatcccgaa
       61 tacggtctga cacctcaccg gtattggtgc gatcataaac cgtcaaatca ccgaatttct
      121 ccagtttatc ccaaccgata tcccccggat tcagggtata gccgtctaaa acaacaagtt
      181 tcatacattc catcctgctt tgacaaaaga taattccgaa aaatacagcc gtttttcaga
      241 ccagcaaaac ttgcagacca atttgcgtta atttttcgac gatttccgga tcgaggccgg
      301 catccgtaat aatcccggaa aaactattta aagaagccga tcgacagttt cccgtcgaat
      361 tgaatttgga cgattccgcc acaatataac ttttattact gatttttatc gcaccggttt
      421 tggttaatgc gtcatctatt tcataggtag tagcgctgtt tcgaacaaga tcgattccca
      481 caacaccgat aaaggacaga tcaaactgga accttgacat ccattcgatg gtcgccgcac
      541 cggtaaaccc ttctcgaagc ttattcattt ttccgccgat aaaaatcaac tttacgcggc
      601 tctcaccggg tatttcttcc aaaacattga tcatgttggt cacaatggtg acgtttctgt
      661 ttttccgaac caactggcgg gcaatttcga cattgatgga agagagatcc agaaagacgg
      721 tatcgccgtt tcgaatcagt gtctcggctt tttccgcgat gcgttgtttg acttctatat
      781 tattttcatg ggaatcgtct gccggttggg gaaccgaatt ttgaattaac ggaattgctc
      841 ctccgtaagt tcttttcagt aagccttctt tctcaagttt tgcaagatct tttcgaatga
      901 gatccggcgt tacctgatat ctttcgctca aatcctttac caaaaccttt ccgttttgat
      961 gaaggaattc aaggatctcc cgttgtcgtt cctcaacaaa catattcacc tcaagacggt
     1021 cctatgaatt taattgttat tgaatattgt atcatttttg gcgtaaacgt tgtctccccc
     1081 aacaatttct cccattccaa agatcgattt cgttttttaa tgggtaaaat taaatccgta
     1141 tgcagcccat cgggaaagga gttcgaaaat gatcagatat acccgaccgc ttgaggacgg
     1201 aaacaactat tttataccgg aggatcggat tgttcgcatt cccgccggat acaccggcga
     1261 accgatcaac cgtttggctg cttatgaaaa tttacaacaa gatctattga acgatcttga
     1321 gaaaatttcg caagaaatgg caggacttcg agaagaaggg aagataaaat cggttcattt
     1381 caaagaactg atgtctcaaa aaatgatcga cactcggatc atctcacttt ttcaaagcta
     1441 tggattggaa tgagatgaag ggatgaaaaa atccgggaag aaagaatatc gccattcaaa
     1501 taaagaaagc tgtttgcttg atgttataag cttttccgtt tatttggaat aagatcagga
     1561 gaaataaatg gttaatcata catatactga aggaaatt
//
LOCUS       DAOMIV010000502         1588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_92476_length_1588_cov_3.084149, whole genome shotgun sequence.
ACCESSION   DAOMIV010000502 DAOMIV010000000
VERSION     DAOMIV010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92476_length_1588_cov_3.084149"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1469
                     /locus_tag="PKV59_09515"
     CDS             156..1469
                     /locus_tag="PKV59_09515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQP47028.1"
                     /translation="MKWFRFDYKIIIAVLVIAALAWFFFGNSIEIVKPLIKLDQDVKA
                     IGRQKTIGFNFSDSSSGLSRITIEIIQDNKGQILTDKIISPKGKSQETLSLTINSGEL
                     RLHDGPATLKVTAADNSLFRNQAVIIKEIKIDTVPPLINLVKNINYINQGGTGFVAYQ
                     PSKALSSTGVYINDYLSPAHATLIDNKPSYFTYFALPMNADKTSTKIMIFARDEAGNE
                     NTMGVPCVIKEKKFRSDKMNLSENFLQQKMPEFQTMVPALQGKTPLEVFIYVNSQMRK
                     DNFLSIQNICKKSSPKELWEGTFLRMKDAAPMALFGDKRSYIYNGKPVAESTHEGVDL
                     ASNARSPIEAANRGIVAFTGAMGIYGNTVIIDHGLGLFTLYGHLSSIDTTVGKNVAKG
                     EKIGLSGMSGLAGGDHLHFSMIVGGKFVDPKEWWDPHWIKDNITK"
BASE COUNT          489 a          345 c          310 g          444 t
ORIGIN      
        1 agcgctttat cacaatatca aggcataatt attggcatat tcccatgata tttcatggtg
       61 attgtaagat attttccttg caaaagccta taaaagttat tataagccgc gcagtcattc
      121 cgatattaca acatttacaa cattaggaga ttattatgaa atggtttcgt tttgattata
      181 aaattatcat tgcggtttta gtcattgccg ctctagcctg gttttttttc ggcaattcta
      241 ttgaaatagt aaagcccctc atcaaattgg atcaggacgt taaagccatt ggaagacaaa
      301 aaacaatcgg atttaatttt tccgattcca gtagcggtct ttcccggatc acgatagaaa
      361 taatccagga taacaaaggc cagattttga ccgataaaat aatctctccc aaaggcaaat
      421 ctcaggaaac actatcgctc acaattaact ccggagaatt gagacttcat gatggtccgg
      481 ctacacttaa agtcacggcg gcggataatt ctctttttag aaaccaggct gttataataa
      541 aggaaataaa aatagacact gttccgccac tcattaatct tgtaaaaaat attaattaca
      601 tcaatcaggg aggcaccggt tttgttgcct accaaccctc caaagcactt tcttccaccg
      661 gcgtgtatat taacgattac ttatctcctg ctcacgccac gttgattgac aataaacctt
      721 cttatttcac ttatttcgct ttaccgatga atgcagataa aacctcaacc aaaataatga
      781 tcttcgcccg tgacgaggcg ggtaatgaaa acaccatggg cgttccctgt gtcatcaaag
      841 aaaagaaatt ccgctcggat aaaatgaatt taagcgaaaa cttcctgcag cagaaaatgc
      901 ccgaatttca gaccatggtt cctgcactgc aaggtaagac cccgctggaa gttttcatct
      961 atgtaaattc tcaaatgcgt aaagataatt ttttatcgat acaaaacatt tgcaaaaaat
     1021 catccccgaa agaactttgg gaaggcactt ttctcaggat gaaagatgcc gcccccatgg
     1081 ctcttttcgg agacaagcgg agttatattt ataacggaaa acctgtcgct gagtccactc
     1141 atgaaggtgt cgatctagcc tccaatgccc gcagtcccat tgaagcggct aacagaggaa
     1201 ttgttgcttt taccggtgcc atgggcattt acggtaatac cgtaattatt gatcatggac
     1261 tgggactgtt taccctttac ggacatttgt catccattga taccaccgtc ggcaaaaatg
     1321 tggcgaaagg tgaaaaaata ggtctttccg gaatgtccgg cctggcgggc ggagatcatt
     1381 tgcatttcag tatgatcgta ggcggaaaat ttgttgatcc caaggaatgg tgggatccgc
     1441 actggattaa agataatata accaaataaa agaatatcat gggcatgccc cccaaggcaa
     1501 gccataaacc ataattccgc cccaaggggc ggtgtattat accctccgct tttagcggga
     1561 ttgttcgact tacaaatttc aacgcact
//
LOCUS       DAOMIV010000503         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_93464_length_1577_cov_4.722733, whole genome shotgun sequence.
ACCESSION   DAOMIV010000503 DAOMIV010000000
VERSION     DAOMIV010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93464_length_1577_cov_4.722733"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..>1577
                     /locus_tag="PKV59_09520"
     CDS             287..>1577
                     /locus_tag="PKV59_09520"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQP47029.1"
                     /translation="MDDLLVQLVEISKTFPGVKALDHVSLDLRPGEVHALIGENGSGK
                     STLMKILSGVYIKDEGKVIVNGKEMNFQKPRDAQDAGIAIIHQELNLCRHLTVAENIF
                     LGREFQNGIFLDKKHQIEEAQKILDRLEVNLDPNSRVSELTVSKQQMVEIAKAISANA
                     NVVIMDEPTSALTIKEIDDLFSIIKVLRKQGKGIIYITHRLEELNAITDRITIIRDGQ
                     HIITTDFKNLSADDIIRYMVGRELKEKFPRIQMPVGKTIFEVKNLSAGKAVRDVSFTV
                     REGEILGIAGLMGAGRTETVRAIFGADKKDSGQLILNGQEIRIDKPQEAIETGIFCVP
                     EDRKRDGLCISMPVYQNATLPNMEKVTHRGIVNTKKEIMIAENMVKQLTIHTPSVFQI
                     VKNLSGGNQQKIVVGKWLVKPAHVILFDEPTRGIDVGA"
BASE COUNT          504 a          288 c          367 g          418 t
ORIGIN      
        1 ttttcctatc ctgtaagtgg ttcggaatgc tccaattacc ggcgacttct tcagtaaaaa
       61 aaatattcta ccggcaggga gcttagggtc gaaagagcaa acaagcgaaa atactttgat
      121 aaaaagaatt tacatagtta tcgaattatg atatgataaa aatcctgaga tgaaatgaca
      181 aatttcttta agaacagatt tgtcggtaca actgacaaat tttagtttat actcacaaac
      241 tagtcttttg aatatcgact tcaattcctg aaaggggaaa ccggttatgg atgatctctt
      301 agttcagtta gtggaaatat ccaaaacatt cccgggcgta aaagcgcttg atcatgtcag
      361 tttggattta agacccggtg aagtacatgc actgatcggt gaaaacggtt ccggcaaatc
      421 aaccctgatg aagattcttt ctggcgttta tattaaagat gaaggaaagg tgattgtcaa
      481 cggaaaggaa atgaattttc aaaagccgcg cgatgctcag gatgccggaa ttgcgatcat
      541 ccatcaggaa ctgaatttat gtcgtcatct gactgtggct gagaatatct ttttaggcag
      601 ggaatttcaa aacgggattt ttctcgataa aaaacatcaa attgaagagg cacaaaagat
      661 tttagaccgg ttggaagtca atctggatcc gaattctcgt gtctccgaat tgactgtctc
      721 caaacaacag atggtggaaa ttgccaaagc tatttccgca aatgcgaatg tagtgatcat
      781 ggatgagccg acttccgctt tgacgatcaa agaaatcgat gatttattca gtattatcaa
      841 agttttgaga aagcaaggga agggaatcat ctatattacc catcgtttgg aagaactcaa
      901 tgcgattacc gatcgcatta cgatcatccg ggacgggcaa catatcatta caacggattt
      961 caagaatctt tccgccgatg atattatccg ttatatggtc ggccgggaat taaaagaaaa
     1021 attcccgcgg attcaaatgc cggtcggaaa aaccattttt gaagttaaaa atctctctgc
     1081 ggggaaagct gtccgagatg tcagtttcac tgttcgggaa ggcgaaattt tggggattgc
     1141 cggtttgatg ggtgcgggcc gaacagagac ggttcgggca attttcggtg cagataaaaa
     1201 agattccggt cagttgattc taaacggtca agaaatcagg atcgataaac ctcaagaagc
     1261 aatcgaaacg ggaattttct gcgttccgga agaccgaaaa cgggacggac tatgcatttc
     1321 catgccggtc tatcaaaatg ctacgctgcc gaatatggaa aaggttacac atcgcggcat
     1381 tgtcaataca aaaaaagaaa taatgattgc tgaaaatatg gttaaacaat tgaccattca
     1441 cactccttcg gtatttcaga tcgttaagaa tctcagcggc ggaaatcagc aaaaaatagt
     1501 ggtcggaaaa tggctggtga aaccggctca tgtgattttg tttgacgaac cgacgcgggg
     1561 gattgatgtc ggagcga
//
LOCUS       DAOMIV010000504         1576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_93584_length_1576_cov_3.188692, whole genome shotgun sequence.
ACCESSION   DAOMIV010000504 DAOMIV010000000
VERSION     DAOMIV010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93584_length_1576_cov_3.188692"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            302..766
                     /locus_tag="PKV59_09525"
     CDS             302..766
                     /locus_tag="PKV59_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HQP47030.1"
                     /translation="MIIDHISHIEKYGSFGPGFESGIRFLLQHQNDPAEPVARYKLDE
                     NVDVMVNQYETKYPEESNFEAHNKYIDVQFMLKGDELVGWAPREKLSEISAAPEKDYY
                     ELFGESDYFPLSEGYFMIFFPEDAHQPGLVKDHPEQVTKIVLKVRVEGNLPL"
     gene            917..1129
                     /locus_tag="PKV59_09530"
     CDS             917..1129
                     /locus_tag="PKV59_09530"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQP47031.1"
                     /translation="MTDSVYKIIELVGTSTESWEKAAKNAIETAEKTLEDLRVAEVEK
                     MDIQLYEGSFVYRTKLKISFKYHSKE"
     gene            1287..>1576
                     /locus_tag="PKV59_09535"
     CDS             1287..>1576
                     /locus_tag="PKV59_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47032.1"
                     /translation="MCDFYLSSNLHGSVQTDQSKILFSPENNEENEIINIYPDVCFQT
                     FEGFGGAITDSSGYVFSLMNSIQKEQLLHQYFSAREMNYQFVRIPIDSCDFSV"
BASE COUNT          520 a          252 c          327 g          477 t
ORIGIN      
        1 cgaatgttca tattcagatt tccgggcaat tggtggaacg gcacagtaca agaacatacc
       61 aagctgattg aaaatcgaag gacttttgac tcgaatctgt ccttttcatc cggatcgttt
      121 gaagatgaat atttcaatag gtttgttgga tgaagaattt ttattttcaa cagatttatt
      181 cccattctta ttttttatac acaaataaaa aaaatcatgg aaagaattgt ccgtgggtta
      241 taatttttaa actctctaaa ttgaatgagg agagtccatt cacggaacga aggaggcatt
      301 catgattatt gatcatatta gccatatcga aaaatacgga tcttttggac ccgggtttga
      361 aagcggtatt cgtttcttat tacagcacca aaatgatccg gcagaaccgg ttgcccgtta
      421 taaattggat gaaaatgtcg atgtcatggt gaatcaatat gagacaaaat atccggaaga
      481 aagtaatttt gaagcccata acaaatatat tgatgttcag tttatgctga aaggagatga
      541 attggtcgga tgggcacctc gggagaaact ctcggaaatc agtgcggcac cggaaaaaga
      601 ttattatgaa ttatttggcg aaagtgatta tttcccgttg agtgagggat attttatgat
      661 ctttttcccg gaagatgcac atcaacccgg gctggtcaag gatcatcccg aacaagtgac
      721 taagatcgtt ctaaaagtac gggtggaagg gaatttgcct ttataaaaaa cggcatttga
      781 gttgcttatt gtaataaatt ctttttcatg tatgataaag atatgaaacg ataattttgt
      841 tgaaaatcgg agaggctttc atatcaaacg ggatcaattc ctcaaaaaga gggaaaacga
      901 aaggagtgta actcaaatga ccgacagtgt ctataaaatc atcgaattag ttggaaccag
      961 caccgaatcc tgggaaaaag cggcgaaaaa tgcaatcgaa acggctgaaa aaaccttaga
     1021 ggatttgcgg gtggcggaag tcgaaaaaat ggatatacag ctctatgaag gttcttttgt
     1081 ttatcgcacg aaactaaaaa tctctttcaa atatcacagc aaagaataat gtttttgtcg
     1141 caaaaaatga ttattaaacc ccatcagcaa tgatggggtt tctttctgct cggatttgag
     1201 tgaaaaattg tttcggatat caaatgatat aatcagccag tcaatcttga atgcaaaaaa
     1261 taattcgggg gtgaaaatat tttcatatgt gtgattttta tttatcaagc aaccttcacg
     1321 gcagtgttca aacagatcaa tctaaaatcc ttttttctcc cgaaaataac gaggaaaatg
     1381 agattatcaa tatttatccc gatgtttgtt ttcaaacatt tgagggcttt ggcggtgcaa
     1441 taacagattc atccggatat gtcttttctt tgatgaattc gatacagaaa gaacagctgt
     1501 tgcatcaata cttttctgct cgagaaatga attatcaatt tgttcggatt cctattgaca
     1561 gctgtgactt ttcggt
//
LOCUS       DAOMIV010000505         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_93724_length_1575_cov_2.240789, whole genome shotgun sequence.
ACCESSION   DAOMIV010000505 DAOMIV010000000
VERSION     DAOMIV010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93724_length_1575_cov_2.240789"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            330..1067
                     /locus_tag="PKV59_09540"
     CDS             330..1067
                     /locus_tag="PKV59_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47033.1"
                     /translation="MFHKCKAVFFISLCLIIACLSSMGPAFYTDESEDSDLIVDGVFE
                     EKFQDDFWDRGWQKLILFGNPEGNYYSLNRKRLSLELPSTQTAVVLTNPNVSFSDVLV
                     EATFENILSNVASYAVICRYSEDGWYELRVNVSGPEAGSYKLLKYDFYRKEQYKNPYV
                     LLHKGMDRIFSVDIKLGLNVKNKLGLLCEGDEIKAYINDKEQFPIKNGKITDDQFEEG
                     SVGFSAQTYGEGLAKIDIVKFSAKEVQ"
     gene            1353..>1575
                     /locus_tag="PKV59_09545"
     CDS             1353..>1575
                     /locus_tag="PKV59_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQP47034.1"
                     /translation="MIDVNELRKGVTFEIDGELLKVLDYEHNKSGRGNASIKVKARNL
                     RTGATYERTFTSGNRVQDVQLDFETVQFLY"
BASE COUNT          485 a          284 c          331 g          475 t
ORIGIN      
        1 tctccaaacc ggagactgtc tattgtgctt ccattccatt ggggaaatat aaagcgatgg
       61 atcacgtcaa tttggattta cctccctacg gaattatttc catgacatta accgcttcga
      121 atcggtaagc ttttaacctc gaaaaccctc ttcgtatact caagcggatt tttcggctat
      181 tcggtgcctt tgattgttct acattttcaa tcgctccgcc gaatatctgg acaactgttg
      241 aaaaaatggt ataaattatt tacctttata tttaacttcc ttcagaagtt tgtaacgtca
      301 atcgaatcgt tttagagata agaggggata tgtttcataa atgtaaagcc gtatttttta
      361 tatcgctttg cctgatcatt gcctgcttgt cctcaatggg cccggcattt tatacggatg
      421 aatccgaaga ttccgatctg attgtcgacg gggtatttga ggagaaattt caggatgatt
      481 tttgggatcg cggttggcag aaattaatcc tcttcggaaa tccggaggga aattactata
      541 gtttgaatcg aaaacgatta tcgttggagc ttccatccac acaaaccgct gtggtactta
      601 ccaatccgaa cgtgagtttt tccgatgtac tggtggaagc cacttttgaa aatattcttt
      661 ccaatgtagc ctcctatgcc gtaatttgtc gatattcgga agatggatgg tatgaattac
      721 gcgtgaatgt ctctggcccg gaagccggtt catataaatt attgaaatat gatttttatc
      781 gaaaagaaca atataaaaat ccatacgttt tattacataa gggcatggac cgtatttttt
      841 cggtggatat taaactgggc ttgaatgtaa aaaacaaatt gggattactt tgcgaagggg
      901 atgaaataaa agcatatatt aatgacaagg aacaattccc gattaaaaac ggcaaaatta
      961 ccgatgatca atttgaggaa gggtcggtcg gcttttcggc tcaaacctac ggcgaaggat
     1021 tggcaaagat tgatattgta aaattttccg cgaaggaagt tcagtaagat cttccgttaa
     1081 gttacacata agatacacaa aaaagagtgc aaaaattttt gtgctctttt gctaagaaaa
     1141 cacgttatcc aatcatatat agtgctttgg aggaaataaa ctgtcctccg aaagcgaaaa
     1201 gttctttatt ccggttcgcc gcaaggacag cattcattca tgtttcaagc ggctgatcgt
     1261 cgattatgcc aatttactga tataatctaa aagctacgga aggaaaaagt tctttcaagc
     1321 ataaaagaga ctgaatacag aaggattttt ttatgatcga tgtaaatgaa ttacgaaaag
     1381 gtgtcacatt tgaaattgac ggcgaattac tgaaggttct ggattacgaa cacaataagt
     1441 cgggtcgcgg gaatgcaagc attaaagtaa aagctcggaa cctgcgcacc ggcgccacat
     1501 atgaaagaac attcacttca gggaaccgag ttcaggatgt acagcttgat tttgaaaccg
     1561 ttcaatttct ttatg
//
LOCUS       DAOMIV010000506         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_93965_length_1572_cov_3.234674, whole genome shotgun sequence.
ACCESSION   DAOMIV010000506 DAOMIV010000000
VERSION     DAOMIV010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93965_length_1572_cov_3.234674"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..>1572)
                     /locus_tag="PKV59_09550"
     CDS             complement(379..>1572)
                     /locus_tag="PKV59_09550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQP47035.1"
                     /translation="ILAGIVYLCCGPILQAHVYGLSEAFSLIFMLSALWMLFDFSEKK
                     RSFPSWLFLGLWIGLLTLVRYAGASILVTAILFCLVSAADMRKRLSRIGGMLLGFLIP
                     FGGWWLRNSQYNESAVNRSLSIHLPGNDVINEGIRTLTEFFIPEAFGIVQKAIRFWKI
                     LWIILLLLLFVWLLRQFIAYLTGKKQELSGIFLFALAAGVYLLVLIGVSVFIDGSTVF
                     DNRMLLPFFICIAGLILSTVGYLIRQKKISSVFGILVIVVFSVFLFEDESDLIKTFHK
                     DGQGFAGSVWTESETAQAAEKLPQGKTFYSNRQTYLWLMQDMPAYILPILRDAALQQD
                     NEDFEKEKEQMQQEIKNGEAYAIVFNYQDMLQDSSDKEWLDSLFEGVPIYGSYADGVI
                     FGIKN"
BASE COUNT          535 a          341 c          295 g          401 t
ORIGIN      
        1 ccatcaatcc gccggttcgc agaatgtaag ggatatcatg gttaaccgca ccgttattga
       61 taatttgagc gactaaaaat ggcaggacaa tgtctcctgt cgcttcgatc accataaata
      121 tagggccaaa caaaaaatat tttttataag gttttgcgta ttttagaaaa ctattcaact
      181 gaaatgatct ccgacttttt gattctaaac caatttcagc tatttttgct tgaaattagt
      241 tattaatggc ggcttatcag gaacagaaaa gaaaataatt gatcaatcaa acacgataag
      301 aaaacaataa agataatgcg aagcatttct taagaataag atttaaatag cacgaataat
      361 ctcataagac aaaaagttct aattttttat accgaatata accccatcgg cataactgcc
      421 gtaaatcgga acaccttcga aaaggctgtc aagccattcc ttatccgaac tgtcttgcag
      481 catatcctga tagttgaaga caatcgcata tgcttccccg ttttttattt cttgctgcat
      541 ttgttctttt tctttttcaa aatcctcgtt atcctgttgc aatgccgcat cccgaaggat
      601 cggaagaata tacgccggca tatcttgcat caaccaaaga taagtttggc gattgctata
      661 aaaggttttt ccttggggta gtttttcggc ggcttgagcg gtttccgatt ctgtccacac
      721 cgatccggca aatccctgtc cgtctttatg aaaagtctta attaggtcgc tttcatcttc
      781 aaacaagaat accgaaaaaa cgacgattac caggatacca aagacagatg aaatcttttt
      841 ttggcgaata aggtatccca ccgttgatag aattaaaccg gcgatacaaa taaaaaacgg
      901 taaaagcatc cgattatcaa atacggtcga accgtcgata aaaacggaaa ccccgattaa
      961 taccaaaaga taaacgccgg ctgccaacgc aaacagaaaa atgcctgaaa gttcttgttt
     1021 ttttcctgtc agataagcga tgaattggcg caacaaccag acaaagagca agagcagaag
     1081 gatgatccaa agaattttcc aaaaacgaat cgctttttga acgatgccga aagcttccgg
     1141 gataaagaac tctgtcaacg ttcgaatccc ctcgttaatt acatcgttac cgggcaaatg
     1201 gattgacaaa gaccggttga cagcgctttc gttatattga gaatttcgca gccaccatcc
     1261 gccgaaagga ataagaaatc ccagcagcat ccctccgata cgagaaagcc gttttctcat
     1321 atccgcagcg gaaaccaaac aaaacagaat ggctgttacc aagatcgatg ccccggcata
     1381 acgaaccagt gttagaagtc cgatccaaag accgagaaac agccaagatg ggaaagagcg
     1441 ttttttctca ctgaaatcaa agagcatcca taaggccgat aacataaaaa tcagagaaaa
     1501 agcttcactc aaaccgtata cgtgagcttg taaaatcggt ccgcagcaaa gatagacaat
     1561 gccggccaaa at
//
LOCUS       DAOMIV010000507         1568 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_94352_length_1568_cov_2.489095, whole genome shotgun sequence.
ACCESSION   DAOMIV010000507 DAOMIV010000000
VERSION     DAOMIV010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94352_length_1568_cov_2.489095"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKV59_09555"
     CDS             <1..480
                     /locus_tag="PKV59_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQP47036.1"
                     /translation="HKHYAKVAYKLSLKKGEKTGTQIVRHRVLLLVSGVHQGTLKALR
                     YARTISDDITAVHVAVDPEEAEKVMKGWQEWGDGYRITFLESPYRVLIEPLVGYIESL
                     LKKLNPNELITIVVPQFVSRDPAVSILHARAADTLRKALIHYQNVVIIEVPYNIDEL"
     gene            511..1182
                     /locus_tag="PKV59_09560"
     CDS             511..1182
                     /locus_tag="PKV59_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HQP47037.1"
                     /translation="MNVIVIGCGKFGTSIASELEHAGYDVVVVDKDYQSFADLPDSFH
                     GRRINGDAMDLEVLERAKIKDADAVILTSQDDTTNLVLAHMFKNHFNIDNVISLNTDP
                     EFRFYYDMTGNNTISMVSWGTLKIEEILMKKSKPSDDSIETTPIEIFNVVLPDIGRPY
                     SAIELQKVPGSIIFAITHDHITSKFLDKTEVKPGDILHMSSTMEGLRNFYDYLDQAAQ
                     KEKSE"
     gene            1179..>1568
                     /locus_tag="PKV59_09565"
     CDS             1179..>1568
                     /locus_tag="PKV59_09565"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HQP47038.1"
                     /translation="MNILIASAGRTSSQLAKVLLKQNHNVSVIESRPEVLQYVHKELP
                     TDVIFEGEILDPDTLEHAGIKDANVLVASTDDDKVNLLLCYIAKERYNVPRTIARVNN
                     SKHAWLFDENFHVDYALDQATIFSMMIE"
BASE COUNT          456 a          304 c          364 g          444 t
ORIGIN      
        1 cataagcatt atgccaaagt agcttataaa ctctctctta aaaaaggaga aaaaacaggt
       61 actcaaattg ttcgccatcg agtcttatta ttggtaagcg gtgtccatca aggaactttg
      121 aaagccctgc gatatgctcg gacgatttcg gacgacatca ctgcggttca tgtcgcagtg
      181 gatccggaag aagccgaaaa agtcatgaaa ggttggcagg aatggggaga cggatatcgg
      241 attaccttcc tggaatcacc ttatcgggtc ttgattgagc cgttggtcgg gtacattgaa
      301 tctctgttga aaaagctgaa tcccaacgaa ttaattacga ttgttgttcc gcaatttgtc
      361 agtcgcgatc cggcggtttc catcttgcat gcccgagcgg cggatactct tcgaaaagct
      421 ttgatccatt atcaaaatgt ggtgattatc gaagtacctt ataatattga tgaactatag
      481 attattcctt cggaggagag agattttttc atgaatgtca ttgtgatcgg ctgcggaaaa
      541 tttgggacaa gcattgcctc ggaactggag catgcgggtt atgacgttgt agtcgtcgat
      601 aaggattatc aaagttttgc ggatttgccg gattcctttc acggacggcg gattaatggc
      661 gatgccatgg atctcgaggt cttggaacgg gcaaaaataa aagatgccga tgccgtgatt
      721 ctgacttcgc aggacgatac aacgaatttg gtcttggctc acatgtttaa aaaccatttt
      781 aatatcgata atgtcatctc cctgaatacc gatcccgagt ttcgtttcta ctatgatatg
      841 acgggaaata acaccatttc aatggtatcg tggggaactc tcaagatcga agaaattctg
      901 atgaaaaaat ccaaaccgtc cgatgattcg attgaaacca cgccgattga aatttttaat
      961 gttgttttgc ccgatatcgg aaggccatat tcagcgatcg agctgcaaaa agttcccgga
     1021 agcatcattt ttgcgattac acatgatcat attacgtcga aatttttaga taagaccgaa
     1081 gtaaaaccgg gagatattct tcatatgagc tccaccatgg agggattgcg taatttctat
     1141 gattatcttg atcaggcggc tcaaaaggag aaatccgaat gaatatatta atcgcttcag
     1201 ccggcaggac ctcatctcaa ttggcgaaag tgctattaaa acaaaatcac aacgtatctg
     1261 taattgaatc acggccagaa gtcttgcaat atgttcataa agaattgccc accgatgtca
     1321 tttttgaagg ggagatttta gatccggata cgcttgaaca tgccggaatc aaggatgcga
     1381 atgttttggt ggccagtacg gatgatgata aagtcaattt gttgctttgc tacatagcga
     1441 aggaacgtta taacgttcct cggaccattg cgcgagtaaa taattccaaa catgcctggt
     1501 tgttcgacga aaattttcat gtcgattatg ccttggatca ggcgaccatt ttctccatga
     1561 tgatagag
//
LOCUS       DAOMIV010000508         1565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_94608_length_1565_cov_3.129139, whole genome shotgun sequence.
ACCESSION   DAOMIV010000508 DAOMIV010000000
VERSION     DAOMIV010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94608_length_1565_cov_3.129139"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKV59_09570"
     CDS             <1..430
                     /locus_tag="PKV59_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HQP47039.1"
                     /translation="LAAVIGHNWPIYLNFHGGRGLSPFLGNLLALFPLGCFIMLLSLG
                     IGCLLTFAPLPLITILLMPVFANIFNGPNAILGLSLGMLLITVVKRLEANRRPLPKDP
                     EERKKVIFLRIFYDRDIPDQKEWIRREPTNQKSVPQHKEP"
     gene            431..673
                     /locus_tag="PKV59_09575"
     CDS             431..673
                     /locus_tag="PKV59_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HQP47040.1"
                     /translation="MKSLPKTGSQYVFGSCGLKADDVVWQTLPNGRIEIRYTFQLFQE
                     GPKGYVHGGASAALMDEALGIAVWESGFHGATVILN"
     gene            863..1540
                     /locus_tag="PKV59_09580"
     CDS             863..1540
                     /locus_tag="PKV59_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47041.1"
                     /translation="MKLSGKITWVGIYAIAMAFLETAVVVYLRRIYGITDLMLSVPPF
                     DPQIAAVELGRELATLVMLLAIGWVAGERIQSKLSFALIAFGIWDIFYYIWLALVISW
                     PRSIFDTDLLFLIPLPWWGPVIAPVLISLLMIIGGILIVRREDQGIIVRLRISDGIFL
                     GLGILIMLYVFMQDAISILPATSEELSSLKPSSFNWFVFMIGYILSAITVWRIVKKKP
                     SHLEKNI"
BASE COUNT          382 a          292 c          383 g          508 t
ORIGIN      
        1 attagcagca gttatagggc ataattggcc tatctatctt aattttcacg gtggtcgtgg
       61 gctgagtcct tttttaggaa atctattagc tctttttcct ttaggttgtt tcattatgct
      121 tctctcactg ggaatcggtt gtctgttgac ttttgcgccg cttccattaa ttacgattct
      181 tttgatgcct gtttttgcaa acatttttaa tgggccgaat gctatcttgg ggttgtcgtt
      241 gggaatgttg ctgattaccg tcgttaaacg tcttgaagcc aaccggcgcc cgctgccgaa
      301 agatccggaa gagcggaaaa aagtgatttt tcttcgtatt ttttacgatc gcgatattcc
      361 cgatcaaaaa gagtggattc gcagagaacc tacgaatcag aaatctgttc ctcaacataa
      421 ggagccataa gtgaagagtt taccgaaaac cggatcacaa tatgtctttg ggtcttgcgg
      481 tttgaaagcg gatgatgttg tctggcaaac acttccgaat ggacggatcg aaattcgcta
      541 tacttttcaa cttttccaag aaggtccaaa aggttatgtt cacggaggtg cctcagcggc
      601 tttgatggat gaagcgttgg gcatcgcggt ttgggagagc ggttttcacg gagcaacggt
      661 tatcttgaat tgaattatcg taaacctatt ccgctcgggg aagaagttcg tgtcgaatgc
      721 cggataacct ctgaagggac tcattcgaga aatgcggagg gcattattta tcgatcggac
      781 ggatcaatag cagttgaagg aaaagggaaa tttgcattag cgcctcaatt atttggaatt
      841 tcatcataaa ggcaggaagc tcatgaaatt atccggaaaa ataacttggg tcggaatcta
      901 tgcaattgca atggcttttt tagagaccgc cgtcgttgtc tatttaagac gtatttatgg
      961 gataaccgat ttaatgctca gtgttcctcc ttttgatccg cagatcgctg cggttgaatt
     1021 gggcagagaa ctggcaacgc ttgtcatgct tttggctatc ggttgggttg ccggtgaacg
     1081 aattcaatca aaactgagtt ttgcgttgat cgcattcggc atttgggata tcttttacta
     1141 tatttggctg gcactggtta tatcatggcc gcgatcgatt tttgataccg atcttctgtt
     1201 tctgattccg ttgccgtggt gggggccggt aatcgcgccg gtgttgattt cgttgctgat
     1261 gattatcgga ggaattttaa ttgtccgaag ggaagaccag gggataatcg ttcgtctccg
     1321 aatctcagac ggcatttttc tcggtttggg aatcttgatc atgctttatg tatttatgca
     1381 ggatgccatt tccattcttc cagctacttc cgaagagctc agttcattaa aaccttcttc
     1441 atttaattgg tttgtattta tgatcggata tattctgagt gcaattaccg tttggcggat
     1501 cgtaaagaag aaaccatctc atttggagaa gaatatataa gtatttttga gatcatctca
     1561 gtgta
//
LOCUS       DAOMIV010000509         1561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_94970_length_1561_cov_3.972776, whole genome shotgun sequence.
ACCESSION   DAOMIV010000509 DAOMIV010000000
VERSION     DAOMIV010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94970_length_1561_cov_3.972776"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PKV59_09585"
     CDS             complement(<1..1093)
                     /locus_tag="PKV59_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HQP47042.1"
                     /translation="MFLPTTKEELNALGWEKADVILINGDSYIDSPYSGIALLGRILE
                     KAGYKVGIIAQPDTSNLNDISRLGEPALFWGVSAGAVDSMVSNYTALKKPRSRDDFTP
                     GGINNRRPDRASIVYTNLIRRAFKNTKPILLGGIEASLRRISHYDYWTDKIRGSILLD
                     AKADYLLYGMADKSILQFAEALKNGQSPQNIRGLVFASHELPENALVIPSLEEVRSDQ
                     KQFIKAFRIFYENCDPITAHRIAQKHDERYVIQNPPAKTLSQQELDEIYSLPFTREQH
                     PYYEAQGPVKALETTRFSVVTHRGCYGECNFCAIAVHEGRTVQWRSEKSILAEIETIS
                     KLPDFKGIITDLSGPTANMYGFECSKKLAQ"
     gene            complement(1252..1322)
                     /locus_tag="PKV59_09590"
     tRNA            complement(1252..1322)
                     /locus_tag="PKV59_09590"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1288..1290),aa:Gln,seq:ttg)
BASE COUNT          452 a          324 c          319 g          466 t
ORIGIN      
        1 cctgagccag tttcttcgaa cattcaaaac catacatatt ggctgtcggt ccgcttaaat
       61 cggtgatgat tcctttgaaa tcaggaagtt tactgattgt ctctatttca gctaagatgg
      121 atttctcgga acgccattga acagttcgcc cttcatgaac tgcaatcgca caaaaattgc
      181 attctccgta acaaccgcga tgagtcacga cagaaaatcg cgtggtttct aaggctttga
      241 ccggtccttg agcttcataa taggggtgtt gttcccgagt gaacggcaaa gaatatattt
      301 catccaactc ttgttgtgaa agcgtttttg cgggcggatt ttgaataaca tagcgctcat
      361 catgcttttg agcaattcga tgagcggtta tcggatcaca attttcataa aaaatgcgga
      421 aagctttaat aaactgcttt tgatccgacc gaacttcttc caacgaaggg atgacaagag
      481 cattctccgg taattcatga gaagcaaaga ccaatccccg aatattttgt ggtgattgtc
      541 cgttttttag agcctcagcg aactggagaa tcgatttgtc cgccattcca tataaaaggt
      601 agtccgcttt cgcatccaac agaatagaac ctctaatctt atcagtccaa taatcatagt
      661 gagaaatacg acgcagactg gcttcgatac ctcccaaaag aatgggtttg gtatttttaa
      721 aagctctgcg tatcaaattt gtataaacga ttgatgctcg atccggcctg cgattgttta
      781 ttccgcccgg agtgaagtca tcgcggctgc gcggcttttt caaagccgta taattggaga
      841 ccatcgaatc aacggcgccc gcagaaactc cccaaaacaa ggcgggttcg ccgagacggg
      901 agatatcatt taaattactt gtgtcgggct gagcaataat tccgacttta tagccggctt
      961 tttcgagaat acgaccgagc agcgcaattc cgctgtaggg tgaatcgatg tatgagtctc
     1021 cgttgatgag gatgacatcc gctttttccc atccgagcgc gttcaattct tctttggttg
     1081 tgggtaagaa cattcaaact gaacctttcg gtttcttata tataaaaatt attcttctat
     1141 ttgattttat cagtcaaagg aaataaggac agccgttttt tcagaaaaac ggacaatctt
     1201 cccaatcgat tatgaacgtt aattcaacat ttctttctgt ggaaataaaa gctgggatac
     1261 ctggattcga accaagatcc acagattcaa agtctggtgt gctgccgttg caccatatcc
     1321 caatgcttga ttgatataac aagcgaagaa cattttatca tgaaacatta aattcgacaa
     1381 aaagagagtg tcaaaggact tcgtttcaaa tttccatgga aatttgaaac agataccttt
     1441 cgaaaaataa ttcagggttt tcctgtttag tcgctgaaat ttgccgattt tgacatagga
     1501 caatttgaat ttcgctgcta taataaacaa gtttgtttgc tttctacatc aaaatatttt
     1561 a
//
LOCUS       DAOMIV010000510         1549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_96124_length_1549_cov_4.883534, whole genome shotgun sequence.
ACCESSION   DAOMIV010000510 DAOMIV010000000
VERSION     DAOMIV010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96124_length_1549_cov_4.883534"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKV59_09595"
     CDS             <1..443
                     /locus_tag="PKV59_09595"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HQP47043.1"
                     /translation="SNPINLQVFWEIVPNYCNMTLATPLLDQKLLTILGIENQSGTSK
                     KLYGPKSINNTIENMYSLNAYMGGALVYYALFLELGEQKFDKAMQTIVQRYAWDVIDT
                     QEFIAVFSEVAGYDLSGLVNKWLVYDVIPEMPGFPTYEEILKSY"
     gene            complement(496..>1549)
                     /locus_tag="PKV59_09600"
     CDS             complement(496..>1549)
                     /locus_tag="PKV59_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQP47044.1"
                     /translation="VTSLSVLYHHFIGTEQIKPAEPMKITQAIPEVLTDFKDIQGQEH
                     VKRALEIAAAGSHNILMVGPPGTGKTLMARALPSILPALTVQEALDVTRIYSIADQLP
                     SDRPLIQSRPFRSPHHTISNVGLVGGGNHPRPGEISMAHHGVLFLDEFPEFGQRVLEV
                     LRQPLEDKVVTISRASGSVTFPANFMLVAAMNPCPCGFYGDPVHPCHCSNLAISNYQK
                     RISGPLMDRIDIHVDVPRIEFQKISSQKDQEPSAAIRARVEKAREIQRQRFSESKNIS
                     CNAEMRPAEIKKFCQPDDEGNRLMRLAMDRYELSARVFHRLLKLARTIADLDGSENIQ
                     SNHIAEAIQYRPRQAE"
BASE COUNT          425 a          366 c          345 g          413 t
ORIGIN      
        1 cttcaaaccc gataaacctt caggtatttt gggaaatagt cccgaattat tgcaatatga
       61 ctctggccac tccccttctg gatcaaaaac tcctgacgat tttgggtatt gaaaaccaat
      121 ccggcacatc aaaaaagcta tatgggccga aatcgattaa taatacgata gagaatatgt
      181 atagtttaaa tgcctatatg ggtggagcgc tggtttatta tgcactcttt ttggaactgg
      241 gtgagcagaa attcgacaaa gcgatgcaga cgattgtcca acgctatgca tgggatgtaa
      301 tcgataccca agagtttatc gcggtattca gcgaagtcgc gggttatgat ttatccggcc
      361 tggtgaataa atggctggtt tatgatgtga ttccggaaat gccggggttc ccgacctatg
      421 aagaaattct aaaatcatat taatgtttgg gattccgtat caatgatttt tataatgaca
      481 aatttattcg ccaacctatt cagcctgccg gggacgatat tgaatcgctt ccgcaatatg
      541 gttggattga atattttccg atccgtcaag gtccgctata gtccttgcca actttaataa
      601 tcgatgaaac acccgagcgg atagttcata ccgatccatt gccagccgca tcagccggtt
      661 cccttcatca tccggttggc aaaacttttt tatttcagcc gggcgcattt cggcgttgca
      721 actgatattc ttactttccg aaaaacgttg ccgctgaatt tcacgggctt tctctacccg
      781 cgcccgaatc gccgccgatg gttcctgatc tttttgactg ctgatttttt gaaattcaat
      841 tcgagggaca tctacatgaa tatcgattcg atccatcaac ggaccggaaa ttcgtttttg
      901 atagtttgaa atcgccaaat tagaacaatg acagggatgg accggatctc cataaaaccc
      961 gcatgggcac ggattcatcg cagccaccaa cataaaatta gccggaaaag ttacggaccc
     1021 cgaagcccgc gaaatggtaa cgactttgtc ttccaacggt tgccgtaaaa cttccaaaac
     1081 gcgctgaccg aattccggaa attcgtccaa aaaaagaaca ccatgatgcg ccatcgaaat
     1141 ctcccccggt ctcgggtgat tcccgccgcc gaccaatccg acgttactga tagtatgatg
     1201 cggggaacgg aacggccggc tttggatcaa aggccggtcg gaaggcaatt gatcggcgat
     1261 agaataaatc cgggtaacgt ccaaagcttc ctgaacggtt aaggccggca agatagaagg
     1321 cagagcccga gccatcagag tttttccggt tccgggaggt cctaccatta aaatattatg
     1381 cgaaccggcg gccgctattt ccagcgcccg tttgacatgc tcctgccctt gaatgtcttt
     1441 aaaatcagtt aatacctccg gaatcgcctg cgtaattttc atcggttcag ccggttttat
     1501 ttgttctgtc ccaataaaat gatgatacaa aacagacaaa gatgttacc
//
LOCUS       DAOMIV010000511         1542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_96838_length_1542_cov_3.418964, whole genome shotgun sequence.
ACCESSION   DAOMIV010000511 DAOMIV010000000
VERSION     DAOMIV010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96838_length_1542_cov_3.418964"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="PKV59_09605"
     CDS             complement(<1..1298)
                     /locus_tag="PKV59_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47045.1"
                     /translation="MNSKNKVLSNHDPDEQRQSIPMEGKPLRDGTAFDILAITAGEGN
                     GWSFSPDVLRAAVPLFDQTQCFIDHIPPKSGTNHSVRDLAGILTAPLWDESVQGIRCR
                     LMPLGPAADLLIETGRQILTAVDTDPKVGFSADLSFRSNGQNVEQIHRIFSVDLVVDP
                     ARGGAFLKAVNAVQKREDLAAESADGRDERITLGNDEILQPDDSQKKLQNYLLETALA
                     SSQLPQPLQAHIRRDFQQRSFEPAELETAIDEARTIVSSLVGGNSVSGISGRVEAMVS
                     SSDQVNAAVHDLLGARRPDELRSVRTQKLSGIRELYTMMTGDLDFHGGYNPQRASLSE
                     SGDLPSVLKNVLNKLVAQRWEELEASGYHWWEKIVTIEHFNSLQNITGILVGEISLLP
                     SVNEGAAYTELGVSDSAETGTWEKYGGYLGLTIEMFERDDT"
BASE COUNT          370 a          429 c          348 g          395 t
ORIGIN      
        1 gtatcgtccc gttcaaacat ttcaatcgtc agtcccaaat agccaccgta cttctcccaa
       61 gtgcccgtct cggcggaatc gctgacacct aattcggtat aagcggcgcc ctcattaacc
      121 gagggcagca ggctgatttc ccccaccaga attccggtaa tattttggag actgttaaaa
      181 tgttcgatcg tcacaatttt ttcccaccaa tgatatccgc tggcttccaa ttcctcccag
      241 cgctgtgcaa ccagcttgtt caaaacattt ttcaaaacag acggcaaatc accgctctcc
      301 gaaagcgatg cccgctgcgg gttatatccg ccgtgaaagt ccagatcgcc ggtcatcatg
      361 gtatacaatt cccgaattcc gctcaacttt tgtgtccgaa ccgaacgtaa ttcatcggga
      421 cgtcgtgctc ccagcaagtc atgaacggct gcgtttacct gatcggaact cgataccatc
      481 gcttccaccc gaccgctgat cccgctgacc gaatttcctc cgaccaatga gctgacgatc
      541 gtccgcgctt catcgatggc tgtttccaat tcggcaggtt caaacgaacg ttgttgaaag
      601 tcccgccgaa tgtgagcttg caaaggctgc ggcagctgag atgaggctaa tgccgtttcc
      661 aataaataat tctgtaactt tttctgtgaa tcgtccggtt gtaagatttc atcattcccc
      721 aacgtaatcc gttcgtcacg cccgtcggcc gattcggcag ccagatcttc ccgtttttga
      781 acggcattca ctgccttcag aaaagcgccg ccgcgagccg gatccaccac caaatcgacc
      841 gaaaagatcc gatgaatttg ctcgacattt tgaccgttgc tgcgaaatga caaatcggcc
      901 gaaaatccga ctttcggatc tgtatccacc gctgtaagaa tctgccttcc ggtttcgatc
      961 aataaatccg cggcaggtcc caacggcatc agccggcagc gaattccctg taccgattca
     1021 tcccagaggg gcgcggttaa gattccggcc aaatcccgca cgctatgatt tgtgccggat
     1081 ttcggaggaa tatgatcgat aaagcattgt gtctgatcaa aaagaggcac agctgctcgc
     1141 aatacatccg ggctgaatga ccaaccgttc ccttcaccgg cagtgatcgc caggatatca
     1201 aaggcagtgc cgtcccgtaa cggtttgcct tccatgggga tggattgacg ctgttcatcc
     1261 ggatcatggt tcgagagtac tttgttcttg gaattcatat ttggttttcc tttcttatat
     1321 agtacatatg ttctatataa gcataaacgg aaccgatcca cgtgcaactg tctttttagg
     1381 gaggatgcca tacggaagga aggttacgca ctgaatgaga aaagagtccc tcaccctgac
     1441 cctctcccgg aggacgaggg ttttgttttc gccgtccctt ttttgagaaa attaatgtag
     1501 gggtcggttc caaaccgacc cttctttgta accatgcccg aa
//
LOCUS       DAOMIV010000512         1536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_97404_length_1536_cov_3.805537, whole genome shotgun sequence.
ACCESSION   DAOMIV010000512 DAOMIV010000000
VERSION     DAOMIV010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97404_length_1536_cov_3.805537"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKV59_09610"
     CDS             <1..357
                     /locus_tag="PKV59_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47046.1"
                     /translation="ADGILNCIERGRSGECYILSNRYYSVPQLLEIFRQVTGKKAVNR
                     YLPMSFAKATAPLAERYYKHLKQPPLYTPYSLHVISANADFSHEKATRELGYTTRPME
                     RTVADTVEWLKQANRI"
     gene            601..1281
                     /locus_tag="PKV59_09615"
     CDS             601..1281
                     /locus_tag="PKV59_09615"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HQP47047.1"
                     /translation="MEENNYCVLIGDIIKSRKYSNRNEIQKKLNAILSETNKKYSDDL
                     AAEFIITLGDEFQGVLNKPDRIFQIIDNIRFSMRPVKIRVGIGIGKITTEIDRSKAIS
                     ADGPAYYAAREMIDDIRKKEKGWKSLSQDIKISLGTDNLEIDLINANLGLTSLLESSW
                     TKKQFEAVRLLLYSDSLQKEAAQKIGITESSMHYRLKSAHYFDYKHSRELISKYLIHL
                     WEEKCSPN"
     gene            1266..>1536
                     /locus_tag="PKV59_09620"
     CDS             1266..>1536
                     /locus_tag="PKV59_09620"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HQP47048.1"
                     /translation="MLTQLILTAFFAHLIGDYYLQSEKTVQSSRTNFRSLLKHSLIYC
                     IPFIVVYFLAVPSLQLMIYFGSLGLVHFLIAGIAYILNRFPAKNIL"
BASE COUNT          461 a          309 c          312 g          454 t
ORIGIN      
        1 gcggatggaa tcctgaattg tatcgaaaga ggccgcagcg gagaatgtta tatcctttcc
       61 aaccggtatt attccgttcc gcagttgctg gagatattcc gtcaagtcac cggcaaaaaa
      121 gcggtaaaca gatatcttcc gatgagtttc gccaaagcca cggctccctt ggctgaaagg
      181 tattataaac atttgaagca accgccgctt tatacgcctt attctttaca tgttatctcc
      241 gctaatgccg acttttctca tgaaaaagcc acccgtgagc tcggctatac aaccagaccg
      301 atggagagaa ccgtcgccga tacggttgaa tggttgaaac aagcaaatcg aatttaaaga
      361 tagattcgta aaccgatcaa tttcggactg tttaaatgat tcgctccgga aataaatttg
      421 cagttaattc ttcaagttta taaaatagca gtaaatccta caatttttca aaattagatt
      481 tattcctata agttttcaga atagtatgtt atactctgat tttagagaag aatgaccggt
      541 atctcattcg aggatgcgga cggaaattct tgttcaagtc tttttggagg gaaaaattcg
      601 atggaagaaa ataattactg cgttttgatc ggagacatca tcaaatcgcg aaagtattcc
      661 aacaggaatg aaattcaaaa aaaattgaat gcgatccttt ccgaaacaaa caaaaaatat
      721 tcggacgatt tagccgccga atttattatc accttgggag atgaatttca gggtgttttg
      781 aacaaacctg accgaatttt ccaaattatc gacaatatcc gattttcgat gcggccggta
      841 aaaatccgtg tcgggatcgg gatcggtaaa attaccacgg aaatcgatcg atcgaaggcg
      901 atcagcgccg acggccccgc ctattatgcc gcaagagaaa tgatcgacga tattcgcaag
      961 aaagaaaaag ggtggaaaag tctttctcag gatattaaga tttccttggg tacggacaat
     1021 cttgagattg atctgatcaa tgccaattta ggactgacat ctctattgga atcttcttgg
     1081 accaaaaagc aatttgaggc ggtccgatta ttgctttatt ccgattctct gcaaaaagaa
     1141 gcagcccaaa aaatcgggat taccgaatcc agtatgcact atcgtttgaa atcggcacat
     1201 tattttgact ataaacattc acgggagctg atctcaaagt atctgattca tttgtgggag
     1261 gaaaaatgct cacccaattg atcttgacgg cttttttcgc tcaccttatc ggcgactatt
     1321 atcttcagtc ggaaaaaact gtgcagtcaa gtcgcacaaa tttccgttca ttgttgaagc
     1381 attccttgat ctattgtata ccgtttattg ttgtatattt tctcgctgta ccgtctttgc
     1441 aattaatgat ctatttcggc tcattgggtc ttgttcattt cctcatcgca gggatcgctt
     1501 atattttgaa tcgtttcccg gcgaaaaata ttctca
//
LOCUS       DAOMIV010000513         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_97784_length_1532_cov_3.280298, whole genome shotgun sequence.
ACCESSION   DAOMIV010000513 DAOMIV010000000
VERSION     DAOMIV010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97784_length_1532_cov_3.280298"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1532)
                     /locus_tag="PKV59_09625"
     CDS             complement(<1..>1532)
                     /locus_tag="PKV59_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47049.1"
                     /translation="IRKRTIAFALLLFGALIGAVGLLLLKYVSPESGADFRQYLGMGL
                     LAIGALLVIFDIISVIRQFGWQIIRKSPAFNLIVFIGALILPLLTAFPVRLMGWDPLD
                     YSSAGIIHTGIVMIVLFVISFLIGIWWNRNVFLKSLIAFYLIFITFYTTLFSNGHGFF
                     TGMVGSLGYWLTQQDVQRGSQPLYYYAAILIPIYEYVAVAGSILGIFFAAKRHLFWAM
                     PTDNLYELTEAKELPETNYAAPVSEGGEIRADSFGDDENMDLHQTLSIDFIDNLESLH
                     FEIEDNEPIEKITEIDELSSVQRQSTVLHQAPSFLLFLFWGLTALAAYSFAGEKMPWL
                     TVHITLPLSLAAAWGIGFLLEKAPWQKIASAKGLIALAALICGTFALSGLLSSLFGPN
                     PAFAGKTLDQLKATNQFIFSAIAFLLVLFVLFRTWKGWSGKDILNSVVIYFVALLCIL
                     EARTAYTASFIHYDYANELLVYAHAAPGPKIVLRQIEEIGARTGEGKSIKVAYDNDAL
                     YP"
BASE COUNT          481 a          352 c          342 g          357 t
ORIGIN      
        1 taaggataca gcgcatcgtt atcgtatgcc acttttatgg attttccctc tccggttcgc
       61 gcaccgattt cctcgatttg cctcaaaaca attttcggac cgggtgcggc atgggcataa
      121 accagcagtt cattggcata atcgtaatgg ataaatgacg ccgtataagc ggttctcgct
      181 tcgagaatgc aaagcaatgc gacaaaataa ataacgacgg aattcagaat atctttaccg
      241 ctccaacctt tccaagttct gaacaggaca aaaagaacaa gcagaaaagc tatcgccgaa
      301 aaaatgaatt gatttgtggc ctttaattga tccaatgttt tcccggcgaa agccggattc
      361 ggtccgaata aagatgagag gagcccgctc aatgcaaagg ttccgcaaat taaagctgcc
      421 aaggcaatca gaccttttgc ggaagcgatt ttctgccaag gagctttttc caacaagaag
      481 ccgattcccc aagcagcggc aagcgacaga ggaagggtga tatgaaccgt gagccacggc
      541 attttttcac ctgcaaaaga ataagccgcc aacgcagtca gtccccagaa aaggaataat
      601 aggaatgacg gggcttgatg caagacggtt gattgtcttt ggacgctgct tagttcgtcg
      661 atttccgtta ttttttcaat aggttcattg tcttcgattt cgaaatgcag cgattccaaa
      721 ttatcgatga aatcgatgct cagagtctga tgtaaatcca tattctcatc gtcgccgaaa
      781 gaatctgccc gtatttcacc cccttccgaa accggagcgg cataattcgt ttccggcaat
      841 tcttttgctt ccgtcagttc ataaagatta tcggtgggca ttgcccaaaa caaatgacgc
      901 ttggcagcga agaaaatccc taaaatactg cctgcaaccg cgacatattc gtaaatcgga
      961 atcaaaatcg ccgcataata gtaaaggggt tgactgcctc tttgaacatc ttgttgtgtc
     1021 agccaatatc ctaacgaacc gaccattccg gtaaaaaagc cgtgaccgtt ggaaaacaga
     1081 gtcgtataga atgtgataaa aatcaaataa aaagcgatca atgattttag aaaaacgttc
     1141 cgattccacc agatcccgat caggaaagaa ataacaaaaa gaacgatcat tacgattccc
     1201 gtgtggataa tacccgcgga ggaataatcc agcggatccc agcccatcaa tcggaccggg
     1261 aaagcggtta ataacggcag aattaaggca ccgatgaaaa cgattaagtt aaaagcgggc
     1321 gacttacgga tgatttgcca accgaattga cgaatgacgg aaatgatatc gaaaatcacg
     1381 agcaaagcgc cgatcgccag aagccccatc ccgagatatt gccggaaatc ggcgccggat
     1441 tccggtgaaa catatttcag cagtagaagg ccgaccgcgc cgattaatgc tccgaaaagt
     1501 aataaagcga aagcaatcgt tctttttcgg at
//
LOCUS       DAOMIV010000514         1531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_97859_length_1531_cov_6.489160, whole genome shotgun sequence.
ACCESSION   DAOMIV010000514 DAOMIV010000000
VERSION     DAOMIV010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97859_length_1531_cov_6.489160"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PKV59_09630"
     CDS             complement(<1..828)
                     /locus_tag="PKV59_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HQP47050.1"
                     /translation="MSFEIKKAAVLGAGVMGANIAAHLTNAGIECYLLDIIPPGGLTE
                     DDKKKGLTEKSPAWRNRFAANGLAGATKSKPASFYAKSNASMVTIGNFEDNLNMLADC
                     DWVCEVVVENLKIKQELFAKVEKIVKPTCIVSTNTSGLPIKDISAKFSKDLKKRFLGT
                     HFFNPPRYMKLMEIIPGVETDKAVTDFMIKFSEEILGKGVVVCKDVPNFIGNRVGVYD
                     LSNAGRVMVEKGMSVEEVDAIVGKVVGRPGTAVFGTMDLVGLDIGVHVAKNLYDAVPK
                     "
     gene            complement(897..>1531)
                     /locus_tag="PKV59_09635"
     CDS             complement(897..>1531)
                     /locus_tag="PKV59_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQP47051.1"
                     /translation="VGLLERRVKEKDSRKKLILKSARSLFFKKGFNQVTVDEIAKASE
                     LGKGSIYLYFDSKEEIYAQILLNDIDNFNNQVAVLLDKKSSPTDLLTEFSYIYIDFFF
                     NDGERFRILMTYMLQPAKMNLPEALNAQILNANARSIDVIGKILQLGVDSKEFSADIN
                     IKQNQNALWGLLNGIISLYIFSGVQAKRKERIYSSTKLALEIFIRGLKKA"
BASE COUNT          422 a          363 c          282 g          464 t
ORIGIN      
        1 tttgggaaca gcatcataaa ggttcttggc aacatgaacc ccgatgtcaa gacccaccag
       61 atccatggtt ccaaagacgg ctgtcccggg acggcctacg accttgccga caatggcgtc
      121 gacttcttca acactcattc ctttttccac catgaccctg ccagcattcg acaaatcata
      181 aaccccgacg cgattaccga tgaagttagg aacgtcctta catacgacaa cacccttgcc
      241 gagaatttct tcgctgaatt ttatcatgaa atctgtgaca gctttgtccg tttccacacc
      301 gggaatgatt tccataagtt tcatgtaacg gggaggatta aaaaaatggg tccccagaaa
      361 acgctttttc aaatctttac tgaatttggc agaaatgtct ttaatgggaa gaccggatgt
      421 gttcgtggaa acgatacagg tcggtttgac aatcttctcc accttcgcaa aaagttcctg
      481 cttgattttc aggttttcga caacaacttc gcaaacccag tcacaatcgg ccagcatatt
      541 caaattatct tcaaagttac cgatggtaac catcgacgca ttgctcttgg cataaaaact
      601 ggccggttta gacttcgttg cgccggctaa tccgtttgca gcgaaacgat tgcgccaagc
      661 cggacttttc tccgttaatc ccttcttctt atcatcctcg gttagtcccc ccgggggaat
      721 gatatccagc agataacact cgatgccggc attcgttaaa tgggccgcaa tgttcgcacc
      781 cataactccc gcgcctaaaa cagctgcttt tttgatctca aatgacatgt ctttcctcct
      841 ttgccttatc ttgcttttaa acttttggct gattataaaa atcctttaaa tattcatcac
      901 gcttttttca aaccccggat gaatatctcc aatgctaatt ttgttgaaga ataaatcctt
      961 tcctttcttt tggcctgcac gccggaaaaa atatagagtg aaataattcc attaagcaga
     1021 ccccataacg cgttttggtt ttgtttgata ttaatgtcag cagaaaattc tttggaatca
     1081 actcccaact gtaaaatctt tccgatgaca tcgattgacc tggcattcgc gttaagaatt
     1141 tgagcattaa gcgcctccgg taaattcatt ttcgcgggct gcagcatata ggtcattaat
     1201 attctgaacc gttcaccgtc attgaaaaag aagtcgatat aaatatatga aaattcagtc
     1261 agtaaatcag tgggagagct ttttttatca agaagtaccg caacctgatt gttgaaatta
     1321 tcaatatcat ttaacagaat ctgagcataa atttcttctt tgctgtcaaa gtagaggtaa
     1381 atggatcctt tccccaattc ggaagctttc gctatctcat cgacggttac ttgattaaaa
     1441 cctttcttga agaataaaga ccgcgctgac ttgagaatta attttttgcg gctgtctttt
     1501 tcttttactc ttcgttccag aagtcccaca g
//
LOCUS       DAOMIV010000515         1530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_97973_length_1530_cov_4.117288, whole genome shotgun sequence.
ACCESSION   DAOMIV010000515 DAOMIV010000000
VERSION     DAOMIV010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97973_length_1530_cov_4.117288"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="PKV59_09640"
     CDS             complement(<1..889)
                     /locus_tag="PKV59_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQP47052.1"
                     /translation="MENYQPEKRKPANKPFGISKTLWITIAVILLLVLFFITFKPVYI
                     MVGLGQQEMGVKIRAGEIIDIVGPGGIYSDFGLYVKMERYNIEGVQFQVSDDEVFTKD
                     MQPVYVEIRGQVFRPALNSVKIIGSDGTVQYFSKDEITKNFVNYRQIYTDNAALETQM
                     DGFSRQAMKVCVGQNDLRGNAVAEGRDILRNCIEAELEKLTRDIGLYVNNIVVTDVKA
                     SEQAMVVINETNQYLLDAEKAKAQSQKLEEEGKANAVQKEAEVRVQQAAAQETARQKV
                     VLAQLQEQELIANQSVLEAE"
     gene            complement(994..>1530)
                     /gene="hpt"
                     /locus_tag="PKV59_09645"
     CDS             complement(994..>1530)
                     /gene="hpt"
                     /locus_tag="PKV59_09645"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQP47053.1"
                     /translation="EENDIAEVLFSEEAIQKRVKELAAQLTKDYEGLDPLMVCVLKGA
                     SFFYADLCRQIKCRIFMDFMAVSSYENESKTTGVVRIIKDLDKNITDRHVVIVDDIID
                     SGLTLKYLKDLLSARKPASIKTISLIEKQLGLKDRIVPDYNGFTMGDHFVVGYGLDYS
                     DYYRNLPYIGILKPETYQ"
BASE COUNT          396 a          359 c          300 g          475 t
ORIGIN      
        1 tttccgcttc caaaacggat tgattggcaa tcaattcttg ttcctgaagt tgtgcaagaa
       61 caactttttg ccgagcggtc tcttgagcgg cggcttgttg aacccttacc tccgcttcct
      121 tttggacggc attggctttt ccttcctctt ctaatttttg cgattgggct ttggcttttt
      181 cggcatcaag cagatactga ttggtctcat ttatgacgac catcgcctgt tcggaagcct
      241 tcacatcggt cacaacaatg ttattgacat ataatccgat atctcgggtt aatttttcca
      301 attcagcttc gatgcagttg cgcaaaatgt cccgcccttc cgcaacggca ttccctctca
      361 agtcattttg tccgacacag accttcatcg cttgccggga gaatccgtcc atctgagttt
      421 cgagcgccgc gttatcggta taaatttgcc ggtagttcac aaagtttttc gttatttcat
      481 ccttgctgaa atattggact gtaccgtcac ttccgataat ctttacactg ttcagtgccg
      541 gacggaagac ctgaccgcgg atttcgacat aaacgggttg catgtctttg gtaaagacct
      601 cgtcatccga tacctgaaac tggacacctt cgatgttgta gcgttccatt ttcacataca
      661 aaccaaaatc actgtagata ccgccgggtc cgacaatatc gataatttca cccgctcgga
      721 ttttgacacc catttcctgc tgaccgaggc cgaccataat ataaacaggt ttaaatgtta
      781 taaaaaataa aacaagaagc aagatcacgg cgatcgtgat ccacaacgtt ttactgatcc
      841 cgaaaggttt attcgccggt ttcctttttt cgggctggta attttccatt ttcatctcca
      901 tgtttttata ttccggtctc aaaacggaca gaactaattt cggaccggtt ggtggaaaaa
      961 aatattaagg caatcaattt atatacgaaa attctactga tatgtttcgg gtttcaaaat
     1021 gccgatatac ggaaggttcc gataatagtc ggaataatcc aagccgtatc ccaccacaaa
     1081 atgatcgccc atcgtgaatc cgttataatc cggaacgatt ctgtctttta aaccgagttg
     1141 tttttcgatt aagctgatcg ttttaataga agccggcttg cgggcgctca ataaatcttt
     1201 taagtatttc aacgtcaaac ccgaatcgat aatgtcatct acgatgacca catggcgatc
     1261 ggtaatgttt ttgtccaaat ctttgatgat acgaacgacg ccggtggttt ttgattcgtt
     1321 ttcgtaactg gaaacagcca taaaatccat aaatatgcgg catttgattt ggcgacagag
     1381 atcggcatag aaaaatgagg cgcctttcag cacacaaacc atcagaggat ctaatccttc
     1441 ataatccttt gtgagttgag ctgccaattc ttttaccctt ttttgaatcg cttcttcaga
     1501 gaaaagcact tccgcgatgt cattttcttc
//
LOCUS       DAOMIV010000516         1526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_98381_length_1526_cov_5.609109, whole genome shotgun sequence.
ACCESSION   DAOMIV010000516 DAOMIV010000000
VERSION     DAOMIV010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98381_length_1526_cov_5.609109"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PKV59_09650"
     CDS             complement(<1..797)
                     /locus_tag="PKV59_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HQP47054.1"
                     /translation="MRKSWWKECVFYQIYPRSFKDSNGDGIGDLNGITEKLDYLKWLG
                     VGAVWLNPVYDSPNDDMGYDIRNYEKIMDEFGTMADFDRLLSEMHRRGIRLIMDLVVN
                     HTSDEHAWFVESGKSRNNPYRDYYIWRPGRNGKEPNNWASFFTPSAWKYDAATDEWYL
                     HLFSEKQPDLNWENQKVRNEIFAMMNRWFDKGIDGFRMDVINLIAKKPGLPDSDKKPN
                     AQGYVMADDCFANQPKVHDYLKEMRKTCFDGRDCMCVGETPFVTKQIA"
     gene            complement(794..>1526)
                     /locus_tag="PKV59_09655"
     CDS             complement(794..>1526)
                     /locus_tag="PKV59_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQP47055.1"
                     /translation="RKNGYHVMISGTNADEKYLKLAQQRNLDGIIIIGMYSHESYADL
                     TKANIPLVLVDSYVDDHHFHTIKINDRYGGYIATRYLIEHGHRKIAFLSGALRESSVN
                     RQRLAGYYDALKEYGIPICKDYVLTDQINFHAGMHLAQTVKNSTDVTAVFCTADVLAL
                     GVIKELNKNERVVPEQISVMGFDNLEIAEFCVPGLTTVGQDIYQKGKTAVELVLNHIK
                     NPNIGKQEISLPVSIVERESVRTIS"
BASE COUNT          415 a          388 c          282 g          441 t
ORIGIN      
        1 gcaatctgtt tcgttacaaa gggtgtttcg cccacacaca tgcaatcccg accgtcaaaa
       61 caggttttcc gcatttcctt taaataatca tgaactttcg gctgatttgc aaaacaatcg
      121 tccgccatga catatccttg agcattcggc tttttatcgc tgtcaggtaa tccgggcttt
      181 ttggcgatga gattaattac atccatccga aaaccgtcaa ttcccttatc aaaccaacgg
      241 ttcatcatgg caaagatctc atttctcacc ttttggttct cccaatttag atccggttgc
      301 ttttcggaaa acaaatgtaa ataccattca tctgtcgcgg catcatattt ccaagccgac
      361 ggagtgaaga aagacgccca gttattcggt tctttcccgt ttcgtcccgg acgccaaatg
      421 tagtaatccc gatatggatt attccggctt tttccggatt caacaaacca agcatgctca
      481 tccgaggtat gattcacgac taaatccatg atcagccgaa taccgcgtcg atgcatctca
      541 gataagagcc gatcaaaatc ggccatcgta ccgaattcat ccataatttt ttcataattc
      601 cggatgtcat accccatatc atcattcggg gaatcataaa ccgggttcag ccagactgct
      661 ccgactccca accacttcag atagtccaac ttctccgtta ttccgttcag atctccgatc
      721 ccgtctccat tactgtcctt aaaactacgg ggataaattt gataaaacac gcattctttc
      781 caccaagatt ttctcatgat attgtcctga ccgattccct ttccacgata gaaaccggaa
      841 gggagatctc ttgtttaccg atattcggat ttttaatatg attcaaaacc aactccacag
      901 ccgttttccc tttttggtaa atatcctgtc cgacagtggt cagcccgggg acgcaaaact
      961 cggcaatttc caggttatca aatcccatca cggaaatttg ttccggaaca acacgctcat
     1021 ttttgttgag ctctttaatg acacccaaag ccagtacatc cgccgtacag aaaacagcgg
     1081 taacgtccgt cgaattctta acggtttgag caagatgcat accggcatga aaattgatct
     1141 gatcggtaag cacataatct ttgcagatcg ggatcccgta ttcttttaat gcatcataat
     1201 aacccgccag acgttgccga ttgacactgc tttcacgcaa agcaccggaa agaaaagcga
     1261 ttttccgatg cccatgttcg atcaaataac gagtggcaat gtatccgccg tatcgatcgt
     1321 tgattttgat tgtatgaaaa tgatgatcat ctacgtagct atcgaccaat accaatggaa
     1381 tattcgcttt ggttaaatcc gcataagatt catgagagta catcccgatg atgatgatcc
     1441 cgtccaggtt gcgttgttgt gccagtttca agtatttttc atccgcattc gtaccggata
     1501 tcatcacatg atagccgttt ttacgg
//
LOCUS       DAOMIV010000517         1524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_98612_length_1524_cov_3.268210, whole genome shotgun sequence.
ACCESSION   DAOMIV010000517 DAOMIV010000000
VERSION     DAOMIV010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98612_length_1524_cov_3.268210"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKV59_09660"
     CDS             complement(<1..562)
                     /locus_tag="PKV59_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQP47056.1"
                     /translation="MLAKVYSCTVTDLDGDIIEVEVDNSSYGLPQTVIVGLADTAVQE
                     SRERVRSAIRNTGLEYPRKKVTINLAPADLRKEGPFFDLPIAIGILAANRIIPLTAFE
                     NSLIIGELSLDGSVRHVRGILPITAKAKESGFSRIYVPACDAREASLIPDIDVFPVTS
                     LSVLYHHFIGTEQIKPAGPMKITQAIP"
     gene            763..>1524
                     /gene="wecB"
                     /locus_tag="PKV59_09665"
     CDS             763..>1524
                     /gene="wecB"
                     /locus_tag="PKV59_09665"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQP47057.1"
                     /translation="MNNRLKVMVVVGTRPEAIKMAPVIKCLQAESHIETIVCLTAQHR
                     EMLDQVLQIFSIPPDIDLNLMTENQTLSDITASILIQMNKVLLERKPDWILVQGDTTT
                     VMAAALAAYYNQMKVGHVEAGLRSWNKWAPFPEEINRKIAGVIADLHFAPTEMARQNL
                     LREGIPPEICHVTGNTVIDAQQLALKIPFEEKGTPLEGIPFGEKEIITVTAHRRENHG
                     KPLVDICNAILKISQIYADRVHIVYPVHLNPIVRKT"
BASE COUNT          466 a          345 c          318 g          395 t
ORIGIN      
        1 ccggaattgc ctgtgtaatt ttcatcggtc cagccggctt tatttgttct gtcccaataa
       61 aatgatgata caaaacagac aaagatgtta ccggaaaaac atcgatatcg ggaattaagg
      121 aagcttcccg tgcgtcacaa gccggaacat aaatccgaga aaatccggat tctttagctt
      181 tggctgtgat cggtaagata ccgcggacat gccgaaccga gccatccaaa gatagttcac
      241 cgataatcaa gctgttttca aaagcggtta atggaataat gcgattggcg gctaaaattc
      301 cgatcgcgat cggcagatca aagaagggtc cttcttttct caaatctgcg ggagcgagat
      361 taatggtgac ctttttccgc ggatattcca agcctgtgtt tcggatcgca gatcttaccc
      421 gctccctgct ttcctgaacc gccgtatccg ctaatcctac aattactgtc tgaggtaaac
      481 cataagaaga attatcgacc tcgacttcga taatatcgcc atccagatct gtaactgtac
      541 aggaatatac ttttgccagc attagggact cccccaaaaa tatgtaaatc tttaaactgt
      601 ctctctttat accatagata agattcgctc aacgatatta tttctcaacc aaaccggtct
      661 ttttatcttt ctcttaaaac ttaaatcatg ataacatttc atttagcaat tcggatcaac
      721 tgataagatt gaaaaaaaga acaggatggt caaataacca ttatgaacaa caggctgaaa
      781 gtgatggtgg tagtaggcac ccgtccggaa gcgattaaaa tggcacccgt cattaaatgt
      841 cttcaagcgg aatcgcatat cgaaacgatc gtctgcctca cggctcaaca tcgagaaatg
      901 cttgatcaag tgcttcagat tttcagcatt cctccggata tcgatttgaa cctgatgaca
      961 gaaaatcaga cactgtcgga tatcacggct tcgattctca tacaaatgaa taaagtactt
     1021 ctggaaagaa aaccggattg gatactggtt cagggggata cgacaaccgt gatggccgca
     1081 gccttggctg cctattataa tcaaatgaaa gtcggtcacg tggaagccgg ccttcgaagc
     1141 tggaataaat gggcgccttt tccggaagaa atcaaccgga aaatcgccgg cgttatcgct
     1201 gatttgcact tcgcccctac tgaaatggca cgccaaaatt tgctccgtga gggaatacct
     1261 ccagaaatct gccatgttac cggaaatacg gtaattgacg ctcagcaact agcattaaaa
     1321 atcccgtttg aagaaaaagg tactccttta gaagggatcc ctttcggaga aaaagaaatt
     1381 ataaccgtta cggcacatcg tagggaaaac catggaaagc cgctagtcga tatatgtaat
     1441 gcaatcttga aaatcagtca aatttatgcg gatcgtgttc atattgttta cccggtccac
     1501 ctgaatccga tcgtccgcaa aacg
//
LOCUS       DAOMIV010000518         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_98797_length_1522_cov_4.609407, whole genome shotgun sequence.
ACCESSION   DAOMIV010000518 DAOMIV010000000
VERSION     DAOMIV010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98797_length_1522_cov_4.609407"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PKV59_09670"
     CDS             complement(<1..1241)
                     /locus_tag="PKV59_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47058.1"
                     /translation="MEKNQRTTCGFIILIGLVYFCLLIPAILTGAADPEMLSVFEVDE
                     YAQYPHVIQMLTPGDTLYHSIRNFFIYQHYFYGFPFYFLSAISVFPLKLLSPNWPENT
                     RLIVCWLRMTINVLPMIFAIGIFTWLITRFKNRLISLSLFVFLLTIPAVLLNNFWWHP
                     DSLSLFFISLVFLFLDLDEFRFGKYFSLAAAACGYTVGIKYQGLYFALAIPLLLIYAI
                     LRKRLSWKKAINKAIIFVVIMMVFFIFSNPLLLLPQERAEIIRTQQLQFEQTGVGIFT
                     IHSNALRNSLLLPSDIRKYYAETWFFILAIIGLLMGFKGEEKQRIQSLLFLSYLMVAF
                     YITAYAATNRLHYFLPVAVPLLGFIADFYFIGKRDFPAIDLKFGTNIRKVMYGIFTLL
                     IAFQFSLNIRLDHSLYRDQLS"
     gene            complement(1334..>1522)
                     /locus_tag="PKV59_09675"
     CDS             complement(1334..>1522)
                     /locus_tag="PKV59_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47059.1"
                     /translation="DYESLWKFSVANYYAGSGCVQRAIASTYLYGYPLIWDTVKNYFG
                     YGCEKTVEYVDRVYSLWG"
BASE COUNT          547 a          303 c          279 g          393 t
ORIGIN      
        1 cttgagagtt gatcccgata caatgaatga tccaatcgaa tgttcagact aaattggaat
       61 gctatcaaaa gggtgaatat tccatacata actttcctaa tatttgtacc gaacttaaga
      121 tcgattgccg ggaaatctct ttttcctata aaataaaaat cggcaataaa ccccaataag
      181 ggtacagcaa cgggaagaaa ataatgtaac cggtttgtcg cagcataggc ggtgatataa
      241 aaagcaacca tcaaatacga taaaaataat agagattgaa tcctttgttt ttcttctcct
      301 ttgaatccca tcaataaacc gattattgcc aatatgaaaa accaagtctc cgcataatac
      361 ttccgaatat cagatggcaa taacaaactg ttacgaagtg cattcgaatg aatcgtaaat
      421 atcccgacac cggtttgctc aaattgtaat tgttgggtgc gaatgatttc cgctctttcc
      481 tgaggcagca acagcagcgg gttggaaaat atgaaaaaaa ccatcatgat cacaacaaat
      541 attattgcct tatttattgc ctttttccat gaaagtctct tccgtaagat ggcgtaaatt
      601 aataaaagag gaattgccag tgcaaaatat aacccctgat attttatacc gactgtatat
      661 ccgcaagccg cggcggcaag cgaaaaatat ttcccgaaac ggaattcatc cagatctaaa
      721 aataagaata ccaaactgat gaaaaacaac gacagactat ccggatgcca ccagaaatta
      781 ttcaacaaaa ccgccggaat cgtcagcaga aaaacaaaca agcttagact gattaatcga
      841 ttcttaaatc gagttatcag ccaagtaaat atcccgatcg caaaaatcat cggtaatacg
      901 ttaatcgtca ttcgcaacca gcaaacaata aggcgggtgt tctcgggcca attcggactc
      961 aacaatttta agggaaagac ggatattgcc gagaggaaat aaaatgggaa tccgtaaaaa
     1021 taatgctgat aaatgaaaaa attacggatc gagtgataaa gagtatcacc gggagttaac
     1081 atctgaatga catgcggata ttgggcatat tcatctacct caaataccga caacatttcc
     1141 gggtctgctg ctccggtcag gattgcggga atcagtaaac aaaaatagac caatccgatc
     1201 agaatgatga atccacaggt ggtacgctga tttttttcca tagctggtac taattataga
     1261 cgaatgattt ttcaaatttt ggaatattca aaacaaattt gggaattatt aaatatcggg
     1321 attagaaatg aaatcatccc cataaagaat agacccgatc gacatactcg actgttttct
     1381 cacaaccgta tccgaaatag tttttaacag tatcccaaat caaagggtat ccgtaaagat
     1441 aggtgcttgc gattgcccgt tggacacatc cggaaccggc ataataattg gcaaccgaaa
     1501 atttccataa actctcgtaa tc
//
LOCUS       DAOMIV010000519         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_98802_length_1522_cov_4.194956, whole genome shotgun sequence.
ACCESSION   DAOMIV010000519 DAOMIV010000000
VERSION     DAOMIV010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98802_length_1522_cov_4.194956"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKV59_09680"
     CDS             complement(<1..202)
                     /locus_tag="PKV59_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47060.1"
                     /translation="MLYLKENHQELDAVVFDLGNVLLDYDPPRFMKDIGVPDQYHELM
                     GNILFNNKNIWPKWDLGVLNREG"
     gene            complement(257..529)
                     /locus_tag="PKV59_09685"
     CDS             complement(257..529)
                     /locus_tag="PKV59_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47061.1"
                     /translation="MAEHNSKKTSSRIEKEASEKPDELSQPATGLSDFIGIVLIAAGF
                     FNVPSLLKKPETQSDWMMLVIVLVFLISGFILLFLPRFLQKKRNKN"
     gene            complement(541..1173)
                     /locus_tag="PKV59_09690"
     CDS             complement(541..1173)
                     /locus_tag="PKV59_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQP47062.1"
                     /translation="MQEEKEIDRDNLEICPNCLEPNEKDLANCAYCGMPLRKQTETAG
                     EALSSESNEIPDSDNSDESAKPNPPKAPAEEKKPNRGMAYAMRGMGIYLIFYAVTEIP
                     RSLKVEDPKNRMLAIVSDIIYMVAGVMMAWPLFKDYQKKRKEKKEKESAELIDSQTPD
                     NIGEETVHSSEIIDGTFKDNLNNAQEQADDSKALEYEKEAVSQRDDHPSD"
     gene            complement(1177..>1522)
                     /locus_tag="PKV59_09695"
     CDS             complement(1177..>1522)
                     /locus_tag="PKV59_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQP47063.1"
                     /translation="LKLGYRGLYLRTAKIDRLNRLGRAAPSNLAAWEDLMIYQPSYKV
                     SVAGTTGAGDACIAGLLAAFLRDTSPDDAVKAANGAGACCCEKPDAFSGIQSWEQMCD
                     RIDNGWEIVQEI"
BASE COUNT          390 a          378 c          283 g          471 t
ORIGIN      
        1 ttccctctct gttcaggacg cccaaatccc acttcggcca tatattttta ttattgaaaa
       61 ggatgtttcc catcagttca tgatattgat cgggaacgcc gatatccttc ataaatctgg
      121 gaggatcata gtccaacaga acattcccga gatcaaatac aacggcatcc agttcctgat
      181 gattttcttt caaatacaac atgtgatttt tataacctca tcttcgaatt acttttcaaa
      241 caatcattac cgcgtcttaa tttttattcc tcttcttttg caaaaaacga ggcaaaaaca
      301 ggaggataaa acccgatatt aagaatacca gaacaatgac aagcatcatc caatcgcttt
      361 gtgtctcagg ttttttcaac aatgagggaa cattgaaaaa accggcggcg attaagacaa
      421 ttccgataaa atcagaaagt ccggtagccg gttgagagag ctcatccggc ttttcggaag
      481 cttccttttc aatccttgag ctcgtctttt ttgaattgtg ttcagccatt ttataaaaat
      541 ttaatccgac ggatgatcat ctctctgaga aacagcctct ttttcatact caagtgcctt
      601 tgaatcatcc gcctgctctt gcgcattgtt taagttgtcc ttgaacgttc catcaatgat
      661 ttcggaagaa tgaaccgttt cttcgcctat gttatccggc gtttgcgaat cgatgagctc
      721 tgccgattcc ttctctttct tctcttttct tttcttctga taatctttga ataaaggcca
      781 tgccatcata acgcccgcga ccatataaat aatgtcgctg acaatcgcaa gcattctgtt
      841 cttaggatct tccactttta aagatcgggg aatttcggta accgcataaa aaatcaaata
      901 aatacccatc ccccgcatgg cataagccat cccccggttg ggttttttct cttccgccgg
      961 agccttggga ggattgggtt ttgccgattc atccgaatta tccgaatccg gtatttcatt
     1021 tgattcggat gaaagggctt caccggcggt ttccgtctgt tttcttaatg gcataccgca
     1081 ataagcacaa tttgccagat ccttttcatt cggttccaga caattcggac agatttcgag
     1141 attatcgcgg tctatttctt tttcttcttg cataggttat atctcctgaa caatttccca
     1201 gccgttatcg atccgatcac acatttgttc ccaactttgt attccgctga acgcatccgg
     1261 tttttcgcaa caacatgcac ctgctccgtt tgcggctttt actgcatcgt cgggagacgt
     1321 atcccgcaga aaagcggcta aaagacccgc aatgcacgca tctccggcac cggttgttcc
     1381 cgcaacggac actttataag acggttgata aatcatcagg tcttcccacg cagccaaatt
     1441 ggagggagct gcccgcccca atcggtttaa tcggtctatc ttggctgtcc gtaagtataa
     1501 tccacgatag cctaatttga gc
//
LOCUS       DAOMIV010000520         1522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_98804_length_1522_cov_4.000682, whole genome shotgun sequence.
ACCESSION   DAOMIV010000520 DAOMIV010000000
VERSION     DAOMIV010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98804_length_1522_cov_4.000682"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..>1522
                     /locus_tag="PKV59_09700"
     CDS             50..>1522
                     /locus_tag="PKV59_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQP47064.1"
                     /translation="MDDHNLMEEDNLFHKKSDYKPSRSITTILIGKPLETAEQEHQTI
                     NKIIGLAVFASDALSSMAYSTQEMMLILAAAGTAAFGYVIPLTLCIVLLLVILTISYE
                     QTIHAYPNGGGAYIVARDNLGDYPAMTAAAALLTDYILTVAVSISSGVAQFTSVFPNL
                     YPNRVIIAVCLVLLVMVINLRGVKESGITFAIPTYIFLVSMGVMVIIGLIQYFTGSLG
                     QVVNPPALQHDGPFVAISTFLILKAFASGTTALTGVEAISNGITAFKEPRTKNAGQAL
                     MMMSVILGTLLMGVSFLTVKTGVIPSEMEANISQVARTIYHSKGIPYLITVASTMIIL
                     VMAANTAFADYPRLSAMLGRDGFLPRRFAWRGSRLVFSTGIMFLAGMAILLIVVFQAS
                     VNRLIPLYAVGVFVSFTLSQFGMAKRWHRAGKLKEGEELKSHGSTISYDKNWHFKMFA
                     NAIGAFCTLVISLIFGITKFTSGAWFVLVLTPFMVFIFSRI"
BASE COUNT          357 a          326 c          369 g          470 t
ORIGIN      
        1 attcaaatga agaatgattt gtttttaact tatttgagga attaaaagca tggatgatca
       61 taatcttatg gaagaagata atttattcca taaaaaatcc gattacaaac cttcccgttc
      121 catcaccaca attttgatcg ggaaacctct cgaaacagct gaacaggaac accaaaccat
      181 taataaaatc atcggattgg cggttttcgc ttcggatgct ctgtcatcaa tggcatacag
      241 cactcaagag atgatgttaa ttctcgccgc agccggtacg gcagctttcg gctatgtgat
      301 tccgttgaca ctctgtattg ttttgttatt ggtgatcttg acgatttcct atgagcaaac
      361 gattcatgct tatccgaacg gcggcggagc ctatattgtg gcgcgtgaca atttaggcga
      421 ctatccggcg atgaccgcgg cagcggctct gttgaccgat tacattctta cggtagcggt
      481 ttccatttct tccggggttg ctcagtttac ttcggtattt ccgaatcttt acccgaaccg
      541 cgtaattatc gcggtttgct tggtactttt ggtgatggta atcaatttgc gcggggttaa
      601 ggaatcgggg attacttttg cgatcccgac gtatatattc ctggtttcga tgggcgttat
      661 ggtgatcatc ggattaatcc agtattttac cggttctctg ggtcaggttg tgaaccctcc
      721 cgccttacag catgacggtc cgtttgttgc gatttcgact ttcctgattt taaaagcttt
      781 cgcgagcgga acaaccgctt tgaccggtgt ggaagcgatt tcgaacggta ttacagcgtt
      841 taaagaaccg cggacgaaga acgccggcca agcattaatg atgatgtctg tcattttggg
      901 aacactcctg atgggagtct cctttttgac cgtcaaaacc ggtgttattc cctccgaaat
      961 ggaagcaaat atctctcagg ttgcaaggac gatctatcat tcaaaaggaa tcccttacct
     1021 gataacggtc gcttcgacga tgattattct ggtaatggct gccaataccg cttttgcgga
     1081 ttatccgaga ctcagcgcca tgctgggacg ggatggtttt ttgccgcggc ggtttgcatg
     1141 gcgcggatcc cgcctggttt tttccacggg cattatgttt ttggcgggaa tggcgatttt
     1201 attgattgtg gtattccagg ccagtgtgaa ccggttgatc ccgctttatg cggtcggtgt
     1261 ttttgtttcc tttacacttt cgcaatttgg gatggcgaaa cggtggcaca gagccggaaa
     1321 attgaaagag ggcgaagagc tgaaaagtca cggttcgacg atcagctacg ataaaaattg
     1381 gcatttcaaa atgtttgcca acgccatcgg agcattctgc acactggtga tttcattaat
     1441 tttcggaatt acaaaattta ccagcggcgc ttggttcgtt ttggtcttaa cccctttcat
     1501 ggtttttatt ttttccagaa tt
//
LOCUS       DAOMIV010000521         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_99037_length_1520_cov_3.281911, whole genome shotgun sequence.
ACCESSION   DAOMIV010000521 DAOMIV010000000
VERSION     DAOMIV010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99037_length_1520_cov_3.281911"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PKV59_09705"
     CDS             complement(<1..1171)
                     /locus_tag="PKV59_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQP47065.1"
                     /translation="MASLIINGKIKRVEPDETIETAILIDKGRILQIADPDRINLKIY
                     DRTEIIDAKGAVILPGFIDAHNHFTMMGTQRSELDLSGIRNREDIIEKIRQENAKLDP
                     QMPIIGFNYEFDFIHQDHRLCAADLEAVAPDRMIQISDRGGHLSVTTPSTLKKAGILL
                     NSREYADCIFPCGGNFGGFTGEICGVANSLLSDYLRIQFRDDQTLKRAWKQASNIATE
                     HGVTSIHALVVEEEFEKLIQFQNQLPIKLKIYTETKNCKAVKTAGLKQIGGCGRVMVD
                     GDTGPYTAAFLEPYRNRPNTKGLLYYSDEELEDYIWNAHSAGLQVALHCIGDAASEQF
                     LNAVENAQQRNPRLIRHRIEHFEFGTSEQITRAKKIGVAISLQPTFNHYWPHTTYF"
     gene            complement(1199..>1520)
                     /locus_tag="PKV59_09710"
     CDS             complement(1199..>1520)
                     /locus_tag="PKV59_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQP47066.1"
                     /translation="AARTIGCTDAQILFRHILPNILRPLIVLATLGIGGAILEGASLS
                     YLGLGAQPMTPEWGSMLSNGRAYLGNAWWISVFPGIGIFLAILSINMIGEGFNEVLDP
                     SNRQ"
BASE COUNT          386 a          344 c          339 g          451 t
ORIGIN      
        1 aaaagtaagt tgtatgtggc caataatgat taaatgtagg ctggagagaa atagcaacgc
       61 ctattttttt tgcccgagtg atctgttctg aggttccgaa ttcaaaatgt tcgattcgat
      121 gtctgatcag acgcggattt cgctgttgtg cattttcgac ggcattcaga aattgttccg
      181 aagccgcatc gccgatacaa tgcaatgcga cttgtaaacc ggcggaatgg gcattccaga
      241 tgtaatcttc gagttcttcg tctgaatagt aaagcaagcc tttggtattc ggtcggtttc
      301 ggtacggttc taagaatgcc gcggtatacg gtccggtatc gccgtcgacc atcacccttc
      361 cgcaaccgcc gatctgtttc agtccggcgg ttttaaccgc tttgcaattt ttggtctcgg
      421 tatatatttt taatttaatc ggtaactgat tttgaaattg aatcagtttt tcaaattctt
      481 cctctacaac caaggcatgg attgaggtca ctccatgttc cgtcgcgata ttcgatgcct
      541 gtttccaagc tcttttcaag gtttgatcat ctctgaattg aatgcgcaga taatcgctta
      601 aaagcgaatt agccacaccg cagatttcgc cggtaaaacc gccgaaattt cctccgcagg
      661 gaaaaatgca atccgcatat tcacgcgaat tcaaaagaat tccggctttt tttaacgttg
      721 atggtgttgt gacggacaaa tgtccgccgc ggtcactgat ttgaatcatc cgatcgggag
      781 caacggcttc caaatcagcg gcgcaaaggc gatgatcctg gtggatgaaa tcaaattcat
      841 agttgaatcc gataataggc atttgagggt cgagttttgc attttcctgc cgtatttttt
      901 cgataatatc ttctcgattt cggataccgc ttaaatccaa ttcagatcgc tgggttccca
      961 tcatcgtgaa atgattgtga gcatcgataa aaccgggtaa aatcaccgca ccttttgcat
     1021 cgatgatttc cgttcgatca taaatcttca aattgattcg atcgggatcg gcaatttgaa
     1081 gaatacgacc tttatcgatt aagatcgccg tttcgattgt ctcatccggt tcgacccttt
     1141 ttatttttcc gttgataatc agacttgcca ttttccgacg cgctttatcg tttgagtatt
     1201 attgcctgtt tgaaggatct aaaacttcat tgaatccttc gccgatcata ttgatgctta
     1261 aaatggcaag gaaaatcccg attcccggaa acacactgat ccaccacgca ttgccgaggt
     1321 atgctctgcc gtttgaaagc atggagcccc attccggcgt catcggttga gcgcccaatc
     1381 ccaagtagct caaagatgca ccttccaaga ttgcgccgcc gatccccaaa gtggccagga
     1441 caattaaagg gcgtaaaata ttcggtaaga tatgtctgaa aaggatttga gcgtcggtgc
     1501 agccgatggt gcgtgctgca
//
LOCUS       DAOMIV010000522         1517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_99320_length_1517_cov_3.525308, whole genome shotgun sequence.
ACCESSION   DAOMIV010000522 DAOMIV010000000
VERSION     DAOMIV010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99320_length_1517_cov_3.525308"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..>1517
                     /locus_tag="PKV59_09715"
     CDS             160..>1517
                     /locus_tag="PKV59_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47067.1"
                     /translation="MTKSGQTEKLLKKNKWSFDDRWIPLVLLFLSVLAFVPLIRSVGF
                     YWDDWPMLWFEITQGPQGFTQAFTSDRPFLGFLYQITGAIVGTDPLNWQIVTVLFRWL
                     VSCAFWWCLRLLWPKEKDKTFWIAALLAVYPGFKQMPIAYVWGNAFIMLFSYVVSYGL
                     MLKAIASAENGNRKAYWLWTIAGVITYAFCTVCTEYYTGLDVVRGIIIWIFLLQYTEF
                     SDRKVSKKIRKTIWYWLPYLAVLILFMFWRIFIFQFHNYQPVLLSRFAANPIQTTGEL
                     FARVIEDAYTATWGAWTEYFSFPTRANLQTASDKMFWIAVVVGFLFIFAALILFRKKS
                     VQNKSDISDATEKKSWIRNAIILGSAAVVFPGIPYLVTSLSPSLDFPRDRWLTAYMFG
                     SCVFLVGLIDSFIRDGIKQKIIVSLFIAMAVGGNLLNTNTYRRDWEAQQDFINQLTTR
                     IP"
BASE COUNT          382 a          310 c          326 g          499 t
ORIGIN      
        1 caaagggatc aattttgtgc aatataaaat aactttgttt cttttagatg tatagtttaa
       61 tttcggatat tatttttcag tattcctcta tttcccttcg aaatgagcac gtgtagaaca
      121 atggtaaaat catgggagta aaatggaaca atacgaaata tgacgaaatc aggtcaaaca
      181 gagaaattgc ttaaaaaaaa taaatggtca tttgatgatc gttggatccc gctggtctta
      241 ctgtttcttt ccgttctcgc ttttgtgccc ttaatccgct cggtcggctt ttattgggac
      301 gattggccga tgttatggtt tgaaattact cagggtcctc agggctttac ccaagctttc
      361 acctcggatc gcccgtttct cggcttcctt tatcaaatta caggcgcaat cgtcggaacc
      421 gatccgttga attggcagat tgttactgtt ttatttcgat ggctggtttc ctgcgcattt
      481 tggtggtgtc tgagattact ttggccgaag gaaaaagata aaactttttg gatagcagcg
      541 ttgctcgccg tttatccggg atttaagcaa atgcccattg cttatgtctg gggaaatgct
      601 tttatcatgt tgttctcgta cgttgtttca tacggattga tgcttaaagc aatcgcatcc
      661 gcagaaaacg gtaaccgcaa agcatactgg ttatggacaa tcgcgggtgt gataacatat
      721 gctttctgca ccgtctgcac ggaatattat acggggttgg atgttgttcg cggcataatt
      781 atttggatat ttctccttca atataccgaa ttctccgacc ggaaagtttc aaaaaagatt
      841 cggaaaacaa tttggtattg gcttccctat ctcgccgtac tgattctgtt tatgttttgg
      901 cggatattta tatttcagtt tcacaattat cagccggtcc ttttatctcg cttcgcagcc
      961 aatccgatcc aaaccaccgg cgaattattt gccagagtca tcgaagatgc gtatacagct
     1021 acttggggtg catggacaga atatttctcg ttcccgaccc gtgcgaatct tcaaaccgct
     1081 tcggataaaa tgttttggat agcggtcgtc gtcggatttc tgtttatttt tgccgcttta
     1141 atccttttcc ggaaaaaatc agtccaaaac aaatcggata tatccgatgc cactgagaaa
     1201 aaaagttgga ttcgaaacgc gatcatttta ggatcggctg ctgtcgtatt tcccggcatt
     1261 ccctatttag tcacctcact ttcgccttcg ttggattttc cgcgcgatcg ttggttgacg
     1321 gcttatatgt tcggaagttg tgttttttta gtcggtttga ttgacagttt tattcgagac
     1381 ggaataaaac aaaaaattat tgtcagcctt ttcattgcca tggctgtcgg cggaaatctc
     1441 ctcaatacca atacttatcg aagggattgg gaggctcaac aggattttat taaccaattg
     1501 acaacccgca tcccgga
//
LOCUS       DAOMIV010000523         1514 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_99601_length_1514_cov_3.704592, whole genome shotgun sequence.
ACCESSION   DAOMIV010000523 DAOMIV010000000
VERSION     DAOMIV010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99601_length_1514_cov_3.704592"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKV59_09720"
     CDS             <1..227
                     /locus_tag="PKV59_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47068.1"
                     /translation="MAMLDCIWTMQQFPLVWMTTGGGPIHATEMLSTYTYKLAFSKYE
                     FSLASASAVIILIFSMVLASVYVRRQKASV"
     gene            229..1071
                     /locus_tag="PKV59_09725"
     CDS             229..1071
                     /locus_tag="PKV59_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HQP47069.1"
                     /translation="MLSKKAKHNFAKTFALVLLILGAIYAGAPIIWMFLCSFKPNTEI
                     FAYPPTFISKTFSLTAYKKILTDSVKVRFFLNSYFVSTAVTLLTLIVSIFAAYSFSRF
                     EFKMKKTLNMIIISVQAVPPITLLIPYFGLIVTLKLYNTYWALILTYMVFTLPYAILM
                     MTGYFNTLPRELDEAVMIDGGSRSVALWRVLFPISIPGIISVGMYTFMQAWNEYLFAL
                     TLTKTVNMRTVPIGIQQLMGQHSYEWNEMMAMSILGCMPVLILFLFFQRYFIGGMTAG
                     SVKS"
     gene            1133..>1514
                     /locus_tag="PKV59_09730"
     CDS             1133..>1514
                     /locus_tag="PKV59_09730"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HQP47070.1"
                     /translation="MLMNMADLLAVANKNYFAVPAFNISDYSMLNGIFEASEEKNAPL
                     IIAIHPDELKFIGVEMVKAIIEKAYKTKLPVCIHLDHGADFNQVILAIQNGFTSVMID
                     GSALPFEENIAICKKVVEAAHAVNV"
BASE COUNT          430 a          283 c          308 g          493 t
ORIGIN      
        1 gtatggcgat gctggattgt atttggacga tgcagcaatt cccgttggta tggatgacga
       61 ccggcggcgg cccgattcat gccaccgaaa tgttaagcac atatacctat aaactggcat
      121 tcagtaaata tgaattttca ttggcgtcag cgagcgccgt tattattttg attttttcta
      181 tggtcctcgc atccgtttac gttcgaagac agaaagcgag cgtttaacat gttgtccaaa
      241 aaagccaaac ataattttgc taaaacgttt gctctcgttc ttttaattct cggtgcgatt
      301 tatgcaggcg ctccgataat ttggatgttt ttatgctctt tcaaacccaa caccgaaatc
      361 tttgcatatc cgccgacttt tatctcgaaa accttttctc ttaccgcata taagaaaatt
      421 cttaccgatt cggtaaaggt acgattcttt ttaaacagtt atttcgtttc gaccgccgta
      481 acactgctca ctttaatcgt ttcaattttc gccgcataca gtttcagccg gttcgaattt
      541 aaaatgaaaa agactctaaa tatgatcatc atcagtgttc aggctgttcc tccgatcacg
      601 ctcttgatcc cttattttgg tcttatcgtt actttaaaac tctataatac ctattgggct
      661 ttgattctga cctatatggt gtttacgctt ccttatgcaa ttttgatgat gaccggatat
      721 ttcaatacgt tgccgaggga actggatgag gctgtcatga tcgacggcgg aagtcgttcg
      781 gtagcgttgt ggagagtact tttcccgatt tctattccgg ggatcatttc ggtcggaatg
      841 tatactttta tgcaagcttg gaatgaatat ttgttcgcat taacactgac aaaaacagta
      901 aatatgcgaa ccgtgccgat cggaattcaa caattgatgg gccagcattc ttatgaatgg
      961 aacgagatga tggctatgag tattttaggc tgtatgccgg ttttgatttt attcctgttc
     1021 ttccaaagat attttatcgg cggaatgacg gccggatcgg taaaaagtta aaatagataa
     1081 agatagaaaa taaaaatact aaaatttata aaatattaga ggataataca atatgttaat
     1141 gaatatggct gacttactgg cggttgcgaa taaaaattat tttgccgttc ctgctttcaa
     1201 tattagtgat tattcgatgc taaacgggat atttgaagct tcggaagaaa aaaatgcacc
     1261 tttaattatt gcgattcatc ccgatgaatt gaaattcatc ggtgtggaaa tggttaaagc
     1321 gattattgaa aaagcatata aaacaaaact gccggtatgt atccatttgg atcacggagc
     1381 tgattttaac caggttattc ttgccattca aaacggcttt acttctgtaa tgatcgacgg
     1441 atccgctctt ccttttgaag agaacattgc gatttgcaaa aaagtagtcg aagcagcgca
     1501 tgccgtaaat gttt
//
LOCUS       DAOMIV010000524         1513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_99703_length_1513_cov_3.497942, whole genome shotgun sequence.
ACCESSION   DAOMIV010000524 DAOMIV010000000
VERSION     DAOMIV010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99703_length_1513_cov_3.497942"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="PKV59_09735"
     CDS             complement(<1..865)
                     /locus_tag="PKV59_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQP47071.1"
                     /translation="MQNKEAVQSPETNQNKDHPQSQAGLLAIHSAVLLFGLSGVIGKS
                     LDLPAVIITFARVFISAIFLFLILTSKKEPVRIRSLRNFSPFLAAGLIMAIHWTTFLQ
                     SIRTSTVAIGTLTFSTFPLFVTFLEPYFFREKLKAGAVLEALGMLLGVFILVPEFEIG
                     NRMTQGILWGMAGSISYAFLSLINRRLAEKYSGEQISFYEQFVATILLLPSLWLIPFK
                     LSGKDIAGLLILGIVCTGIGQALFITGLKNVRVQTAGIISGLESVYGIIFAFLLLGEA
                     PGIREICGGLII"
     gene            947..1489
                     /locus_tag="PKV59_09740"
     CDS             947..1489
                     /locus_tag="PKV59_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQP47072.1"
                     /translation="MNDLEFIKSKKNQNLNGGNMGNLFNGAEFFEMMAANEKAVGDLY
                     RFLAKDAKFGGKFFENMAADEDRHYGIYTSLLKKYQGKKDLMVELAEDRKSYMDLLIK
                     NNYLSDPEKLKERAANAKSKEDIFDIAERIERDSVMFTQEMIDLFPDLDPADFKVVLQ
                     EEKKHLAMVLEVVGAITKVW"
BASE COUNT          481 a          309 c          355 g          368 t
ORIGIN      
        1 gaatgatcaa ccccccgcaa atctcacgga ttcccggcgc ttcccccaac agcagaaaag
       61 caaaaatgat tccgtaaacg gattccaaac cggagataat tcccgccgtc tgtacgcgga
      121 cattttttaa ccccgtaata aacaaggctt gcccgatacc cgtacagaca atccctaaga
      181 taagaagccc ggcgatatct tttccggata atttgaaagg aatcaaccat aacgaaggca
      241 gcaagagaat tgtcgcgacg aattgttcat aaaaactgat ctgttcgccg gaatattttt
      301 cggcgagccg gcggttgatc aacgaaagga acgcataact gatgctgccc gccatgcccc
      361 acaggattcc ctgcgtcatc cggttgccga tctcaaattc cgggaccagg ataaatacgc
      421 ccagcagcat tccgagcgct tcaagaacgg cgccggcttt cagcttttct cggaagaaat
      481 acggttcaag aaatgtgaca aaaagcggaa aagtggaaaa agtgagtgtc ccgatggcta
      541 ccgtggatgt gcgaatggat tgcagaaaag tcgtccaatg gatggccata atcaaaccgg
      601 ctgccaaaaa gggggaaaag tttctcaaag accgtattcg aacgggttct tttttcgaag
      661 ttaaaataag aaacaaaaaa atcgccgaga tgaaaacacg ggcaaaagta atgataacgg
      721 ccggcaagtc aagcgatttt ccgatgacgc cggaaagccc gaataagagt acagccgagt
      781 gaatcgccaa caatcccgct tgactctgtg ggtgatcttt gttttgattt gtttccggcg
      841 attgaacggc ttctttgttt tgcattgtga agacttttct tgcggaaatc ttccctattg
      901 aaggatacta ttacaaccat tataataaat ttatatcgaa agtctgatga atgatcttga
      961 attcatcaaa tcaaaaaaga atcaaaattt gaacggagga aatatgggca acctttttaa
     1021 tggcgctgag ttttttgaaa tgatggcagc taacgaaaaa gctgtcggcg atctgtaccg
     1081 atttctggcc aaagatgcaa agttcggcgg aaaattcttt gaaaacatgg cggcagatga
     1141 agaccgccat tatgggatat atacttcctt acttaagaaa tatcaaggga aaaaagattt
     1201 aatggttgaa ttggcggaag atcgaaagag ctatatggat cttttaataa aaaataatta
     1261 tctttctgat cccgaaaaac ttaaggaaag agcggcgaat gccaagagta aggaagatat
     1321 ctttgatatc gctgaacgaa tcgaaaggga ttcggtgatg tttactcagg aaatgattga
     1381 tttgttccct gatttggatc cggctgattt caaagtcgtt ctgcaggaag agaaaaagca
     1441 tttggcgatg gtgttggaag tggtcggcgc catcacgaag gtgtggtaat agcccttgta
     1501 aaacaagccc acc
//
LOCUS       DAOMIV010000525         1512 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_99769_length_1512_cov_6.217570, whole genome shotgun sequence.
ACCESSION   DAOMIV010000525 DAOMIV010000000
VERSION     DAOMIV010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99769_length_1512_cov_6.217570"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..>1512)
                     /locus_tag="PKV59_09745"
     CDS             complement(275..>1512)
                     /locus_tag="PKV59_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQP47073.1"
                     /translation="MKKIWIVNYYTTPPDYVSNPRHLEFAHYLRKAGYDVTIFSAGYL
                     RDENVDFVPDSRKYIRADYGGQKFIHIKVRHYTGNGMDRMFSIFQFAWRLFRFRKHFE
                     KPDIILHNIHPPFDYPVYWCARKLKVKYIVEAWDLWPDSFIRFGLIGKNNPIAQAAYA
                     VERRLYEKGDKIIFSFEGGPDYLRDRKWTRESGGKIDPAKIFYINNGVNLEKFDQDIQ
                     RHRLSDQDLEDDGYFKVIYLGSIRLVNNIKQLIDAAGILKTHTHIKFLIYGNGSDREY
                     LEQYCKDKKIDNVLFKEKWIPLRYVPYVLSRSSLNILNYQKDFGIYGVSSGKLFQYLA
                     SGKPICANIKMKYCLIERHHLGVAQDMETPQEYANAILSIASLDQLSYQSMCDRAKEV
                     AMAFDFQVLSDQLMSCLEK"
BASE COUNT          444 a          330 c          303 g          435 t
ORIGIN      
        1 tcctgaaggg ttttccgccc aagccaggtc tttgctgact aaataagacg ggagaaccca
       61 aattcaagcg gacgagaaac gcgataaaga taaaaacggg caataatata caaaccgccg
      121 gaagggttaa caccagatca aagatccgct tgccaaaggc aagagatatt tgccacattt
      181 ttttatctat tgatttaacg aaagtcactg acgtttaggc cttttctatt ctatcaaaat
      241 tatttctctt gtcctgtccg ttttgaaaaa ccattcattt ttctaagcac gacatcagct
      301 gatcagacaa aacctgaaaa tcaaaagcca ttgccacctc tttggccctg tcacacatgg
      361 attgatagga tagctggtcc agagatgcaa tagaaaggat agcatttgca tactcctgag
      421 gagtctccat atcttgagca actcctaaat gatgcctctc aatcaggcaa tatttcatct
      481 tgatatttgc gcaaatcggc tttccggatg ccagatactg aaacagtttt ccggaactga
      541 cgccgtagat gccaaaatct ttctgataat tcagaatatt gagagaactg cgagacaaaa
      601 cataaggaac atatcgtagc ggaatccatt tttccttaaa caggacgttg tctattttct
      661 tatctttgca gtattgctca agatattctc tgtcgctgcc gtttccgtaa atcaggaatt
      721 tgatgtgcgt atgagttttc agaatgccgg ctgcgtcaat cagttgtttg atattgttga
      781 ccagacggat ggatcccagg taaatcactt taaaatagcc atcatcttca aggtcttgat
      841 cggaaagccg gtgccgttga atatcctgat caaacttttc cagattcacg ccattgttga
      901 tgtagaagat tttagcggga tcgatcttgc caccggattc tctggtccac tttctgtccc
      961 ttagatagtc cggacctcct tcaaaagaaa aaatgatttt gtcgcctttt tcgtaaagtc
     1021 ttcgttccac tgcgtaggct gcttgggcaa tgggattatt cttgccgatt aatccaaagc
     1081 ggataaagga atcaggccat aaatcccacg cttccacaat atattttact ttaagcttcc
     1141 tggcacacca ataaaccggg taatcaaaag gtggatgaat attatgaaga atgatatccg
     1201 gcttttcaaa atgcttccgg aatctgaaaa ggcgccaggc aaattggaag atggaaaaca
     1261 tccggtccat tccgttgccc gtgtaatgcc tgactttgat atgaataaat ttctgcccgc
     1321 catagtccgc gcggatatat ttcctggaat ccggaacaaa atccacattt tcgtctctta
     1381 aatagccggc ggaaaaaatc gtaacatcat atcccgcttt tcgcaaataa tgtgcaaatt
     1441 ccagatgacg cggattggaa acatagtccg gaggcgtggt ataataattt acaatccaga
     1501 tttttttcat gg
//
LOCUS       DAOMIV010000526         1507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_100297_length_1507_cov_3.272727, whole genome shotgun
            sequence.
ACCESSION   DAOMIV010000526 DAOMIV010000000
VERSION     DAOMIV010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100297_length_1507_cov_3.272727"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            624..>1507
                     /locus_tag="PKV59_09750"
     CDS             624..>1507
                     /locus_tag="PKV59_09750"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HQP47074.1"
                     /translation="MCFMIAVGKKASADGSTMVARNCDSNSTEAQRIIAYPRKKHQPH
                     ELIRIPDSNNVEIPQVEETFAFIAITRYVDGDDIGMVAGGVNEFQLSAGASTGGWLKK
                     EVEDISPMPETVIGDYRMQLILERCKTAREAIEWLGQNVAEYGARTDNYIIADPNEVW
                     LYEEYQDHHWAAVRVPDDSFVVEGNSFRIADFFPEDTANYLCDPDLIPFAIQHGLWDP
                     NSGKPFNVSRAYGTNNRNRPRGKHGEFEQPYYSLHRIWKSISLLNTDLNLDEYEPTQE
                     YPLFVKPSKSITPEMLIHV"
BASE COUNT          520 a          260 c          294 g          433 t
ORIGIN      
        1 cgcagaaaat ctaacgatta ccgattcgct gacgccgctt cttttagcaa cctcagtaat
       61 ggacataaag gcaacttctt cgtaattaga acaaataaaa tcagctacct ttttaaaact
      121 cttactaaat ccgtcttgct ctttttttat gcgctcgaaa atatccatcc cttgcatcgt
      181 ccttacttat aatatattta agtgttactt cgattcaaaa ttatattata tttatcgaga
      241 aaaaggctta attatgaaat aagaagtttt ataataaaaa aattcttcat aaattatcaa
      301 aaaacatata atgaataaaa atgaattttc gaagatctta caagagagat ttatgttaca
      361 atatttgaac aaaagacaaa aaaatttctt aaaattataa taatttaaga attgagaaat
      421 aaattaacaa caagaatatg tgggcatttg gcagcctgat ccattcaggc ctatgaaaca
      481 gccaaaaaga atgaccgggg tacttaagaa tttgagcgaa agagtttgaa tatttctatt
      541 catttgaaaa aatattgtca cataaaggga caaataattc aataaacaat aagaatgaac
      601 taatacaaag agaggaaaaa attatgtgtt tcatgatagc agtgggaaag aaagcgagtg
      661 ccgacggatc aacaatggtt gctcggaatt gtgattccaa ttcaaccgaa gcccaacgaa
      721 ttattgcgta tccacgaaaa aaacatcaac cgcacgaatt gattcgcatc cccgattcaa
      781 ataatgttga gattccgcaa gtagaggaaa catttgcatt tatagctata acccgatatg
      841 ttgacggaga tgatatcgga atggttgccg gaggcgtcaa cgagtttcaa ttaagcgccg
      901 gtgcaagtac gggcggttgg ttgaaaaaag aagttgaaga tatttcgccg atgccggaaa
      961 ccgtgatcgg cgactaccgt atgcaattaa ttttggaacg atgcaaaaca gctcgcgaag
     1021 cgatcgaatg gctcggacaa aatgtagcgg aatatggagc caggaccgac aattatatta
     1081 ttgcggatcc gaatgaggtt tggttgtatg aagaatatca agatcatcat tgggctgctg
     1141 ttcgtgtacc tgatgacagt tttgttgttg aaggtaattc atttagaatc gctgattttt
     1201 tcccggaaga taccgcgaat tatttatgtg atccggattt gattcctttt gcgattcaac
     1261 acggtttatg ggatccgaat tccggaaagc cttttaatgt atctcgtgca tatgggacca
     1321 ataacaggaa tcgtccgcgc gggaaacatg gcgaattcga acagccttat tatagtcttc
     1381 atcgaatttg gaaaagcatc agtttattga acacggattt gaatttggat gaatacgaac
     1441 ctactcaaga gtatccgctt tttgtcaaac cttccaaatc cataactccc gaaatgctga
     1501 ttcatgt
//
LOCUS       DAOMIV010000527         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_100569_length_1504_cov_4.693582, whole genome shotgun
            sequence.
ACCESSION   DAOMIV010000527 DAOMIV010000000
VERSION     DAOMIV010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100569_length_1504_cov_4.693582"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="PKV59_09755"
     CDS             complement(<1..486)
                     /locus_tag="PKV59_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47075.1"
                     /translation="MDQIKKIPGWEKYLELFKRKDRKTDFSSSGLIRAARYPVAAAVY
                     QELNRPVLYITDKPSHATLAFDELGFWLGEDSIMLFPAPDPLFYEQGNWDPATRSSRL
                     RTLLTLAERAIPSASAPANVPFIVTSVRGLMTRTMEKRNFVKSIRTLRINQQITQEEL
                     VR"
     gene            complement(496..1146)
                     /gene="pth"
                     /locus_tag="PKV59_09760"
     CDS             complement(496..1146)
                     /gene="pth"
                     /locus_tag="PKV59_09760"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQP47076.1"
                     /translation="MTRPMEHFFETIKKQREPVVESVGGEPYLLVGLGNPGREYRESR
                     HNFGFMVIDRIAETLRTEFKKSQMKALISVQAYEGKKVILSKPQTYMNCSGQAVIPLL
                     NYYKIPREHLLVIHDDMDLPFGTLRMRPGGGSGGQKGLESIIQLLGTQDFARLRCGIG
                     HPPGQMDVTDYVLNRFSKEESKLLPSVLENATKAALSFVSVGIQEAMTRFNGVVEG"
     gene            complement(1204..1371)
                     /locus_tag="PKV59_09765"
     CDS             complement(1204..1371)
                     /locus_tag="PKV59_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQP47077.1"
                     /translation="MESAILTEVPPEGLRNPATVDLMSLGLKVFLGIVFVILIFFIVK
                     IIHDLRSYRYR"
BASE COUNT          412 a          386 c          301 g          405 t
ORIGIN      
        1 acgaaccagt tcttcctgag ttatctgttg attaattcgc agtgtccgaa tggatttaac
       61 gaagttcctt ttttccatgg ttcttgtcat caatccgcgg accgatgtca cgataaacgg
      121 aacattcgca ggtgcggaag ccgacggaat cgcccgttcg gcgagtgtta aaagtgttct
      181 caagcgcgaa gaacgggtag ccggatccca atttccctgt tcgtaaaata acggatcggg
      241 cgccggaaac aacattattg aatcttcccc gagccaaaag ccgagttcat cgaacgccag
      301 agtcgcatgc gacggtttat ccgtaatata gaggaccggt cggtttaatt cctgataaac
      361 cgcagccgcc accgggtaac gggcagcccg aatgagaccg gaagaactga aatcggtttt
      421 ccgatccttc cgtttgaata gttccagata tttttcccaa ccggggattt tcttaatttg
      481 atccaacaac ctgctttatc cttccacaac cccgttgaat cgggtcatcg cttcctgaat
      541 tccgacggat acaaaagaaa gcgctgcttt ggtcgcattc tccaaaacag atggcaagag
      601 tttcgattcc tctttcgaaa accgattcaa cacataatcc gtcacatcca tttggcccgg
      661 cggatgtccg atgccgcaac gtaagcgagc aaaatcctga gtccccaaaa gttgaataat
      721 cgattctaag cctttttgtc ctccggaacc gcctcccgga cgcattcgta aggttccgaa
      781 cggcagatcc atatcatcgt gaatgaccag taaatgttct ctgggaattt tgtaatagtt
      841 caataacggg atgactgcct gacccgaaca attcatatac gtttgcggct ttgataaaat
      901 tacttttttc ccctcataag cttgaacgga aatcaaagct ttcatctggc ttttcttgaa
      961 ttccgtccgg agcgtttccg caattcgatc gatcaccata aatccgaaat tatggcgcga
     1021 ttcacgatat tcgcgtcccg ggttgcccaa tccgaccagc aaataaggtt cgcccccgac
     1081 cgattcaaca acaggctccc gttgtttttt tattgtttca aagaaatgtt ccataggtct
     1141 cgtcattttc tgtaatcttt gccataaccc gaagaggcag ggagttcgga atgccgtaag
     1201 atttcatcga tatcgataac ttcgcagatc atgaataatc ttcacaataa agaaaattag
     1261 gattacgaag acaatcccta aaaatacctt caatcccaaa ctcatcaagt ccaccgtcgc
     1321 cgggtttctt aacccttccg gtggaacttc cgtcaatatc gccgattcca caaacacttc
     1381 ccgcctttcg tccgattgtc atccgttatc ataacaaccg taacatttta gtttaacatg
     1441 agcaagccga agctgcaaat tttaccgaaa aaaccatgat ttattgatca atatctcgag
     1501 aaat
//
LOCUS       DAOMIV010000528         1504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Flexilinea sp. isolate AMR_MDS_4915
            NODE_100588_length_1504_cov_3.118703, whole genome shotgun
            sequence.
ACCESSION   DAOMIV010000528 DAOMIV010000000
VERSION     DAOMIV010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867074
            Sequence Read Archive: SRR5168926
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Flexilinea sp. (wastewater metagenome)
  ORGANISM  Flexilinea sp.
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae; Flexilinea.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 18:06:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,954
            CDSs (total)                      :: 1,929
            Genes (coding)                    :: 1,920
            CDSs (with protein)               :: 1,920
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 8 of 9
            Pseudo Genes (internal stop)      :: 1 of 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Flexilinea sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100588_length_1504_cov_3.118703"
                     /isolate="AMR_MDS_4915"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2017390"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..>1504
                     /locus_tag="PKV59_09770"
     CDS             339..>1504
                     /locus_tag="PKV59_09770"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF018571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HQP47078.1"
                     /translation="MEKYTEEPTLDLKYLDLLAEKYPNIQEASTEIINYQALLQLPKG
                     TEHFMSDLHGEYEAFIHIMNNASGEIRKKVDSLYGKMLTSVERAQLSTLIYYPEEKLK
                     EIKNNQEININEWYMITIHQLIEIAHLVSIKYSRNKVRKSLPPEYAFIINELINAENT
                     SREEYYESIISTIIEIGKAEDFIVAFSTVIKRLIVDQLHIVGDIFDRGPRPDIIIDSL
                     RAHHSVDIQWGNHDVLWMGAASGSAACIATVLSNSIKYGNLDTLETAYGINLRPLALF
                     AQQQYKGSKRYELKSNDEIGNEDLRLLSKMYEAISVILFKLEGQIILRHPEYNMDDCL
                     LLNHINRKKGTIWIAGKTYPIKIDDLFPMDEESPYQLTSDEQSVLDQLFTTFKN"
BASE COUNT          497 a          297 c          290 g          420 t
ORIGIN      
        1 aagaatattt cttttccgac gatttgctct atgtttgata agggttttct aaaacaataa
       61 attcccgcaa cggatcatca aaggaaaacc gattgaatca cccggaacaa atagggtcgg
      121 ttccggttgc tttacatcgc cccattcttt tcatggagtg tctgtatttt caatcaaata
      181 cccctaataa atagagattt atcttcgaag atgatattga ttcatccgcc aaaatgtgtt
      241 tgcattccgc tttttgctgt ttgagaaata agaaatcaga gattacaata cacttacgta
      301 catcaaattg accatcggaa gaaacaggat aatcaaaaat ggaaaaatac actgaggaac
      361 cgaccttgga tctgaaatat ttggatttgt tggctgaaaa atatccgaat atacaagaag
      421 cctcgactga aatcatcaac taccaggcac tgctccaatt gcccaaggga acggaacatt
      481 ttatgagtga cctgcacgga gaatatgaag catttattca tataatgaat aatgcatcgg
      541 gtgaaattcg caaaaaagtg gattcactct atgggaaaat gctgacctcc gtagaaagag
      601 cccaactttc tacgctgatt tattatcccg aagagaagct caaggaaata aaaaacaatc
      661 aagagatcaa tattaatgaa tggtatatga tcacgattca tcagctgatt gaaattgccc
      721 atttggtatc gattaaatat tcacgcaaca aagtccgtaa atctctgcca ccggaatatg
      781 cctttattat taacgaactg atcaatgccg aaaatacatc ccgcgaagaa tactatgaaa
      841 gtattatttc gaccattatc gaaatcggta aagcggaaga tttcattgtc gctttttcga
      901 ccgttataaa acgtcttatc gtggatcagc tccatatcgt tggcgatatt tttgatcgcg
      961 gaccgcgacc ggatatcatt attgattcgt tgcgtgccca ccactccgtc gatattcagt
     1021 ggggaaatca tgatgtttta tggatgggag ccgcatccgg ttccgccgcc tgtatcgcaa
     1081 ctgttctgag taattcaatc aaatacggaa atttggatac cttggagaca gcttacggca
     1141 tcaatctgcg cccactggca ttatttgctc agcagcaata taaaggatca aaacgctacg
     1201 aattgaaaag caacgacgaa atcgggaatg aagacttacg gcttctatcc aaaatgtacg
     1261 aagcgatttc agttatttta ttcaaattgg aaggtcagat catcctcagg catccggaat
     1321 acaacatgga tgattgtctg ttgttaaacc atataaatcg aaagaaaggg acaatttgga
     1381 tcgccggaaa aacgtatccg atcaaaatcg atgatctttt cccaatggat gaagaaagtc
     1441 cttatcaatt gacttctgac gagcaatcag tcttagatca attgtttaca acatttaaaa
     1501 atag
//
